Stereoselective inhibition of chloride transport by loop diuretics in pancreatic beta-cells.
The effects of loop diuretics on 36Cl- uptake was tested in isolated beta-cell-rich pancreatic islets. Bumetanide reduced the 36Cl- influx and the levorotatory form of ozolinone reduced both the influx and equilibrium content of 36Cl- in the islets, whereas the dextrorotatory form was largely inactive. The data suggest that the beta-cells are equipped with a loop diuretic-sensitive system for 36Cl- uptake and that this system is confined to a sterically well-defined structure.
Sandström
P E
PE
Department of Histology and Cell Biology, University of Umeå, Sweden.
Sehlin
J
J
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002034:Bumetanide / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D004232:Diuretics / Q000494:pharmacology*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008820:Mice, Obese; D013844:Thiazoles / Q000494:pharmacology
D002712:Chlorides; D004232:Diuretics; D013844:Thiazoles; D002034:Bumetanide; C018121:ozolinone