title: Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.
abstract: When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.
authors.affiliation: Department of Medicine, Veterans General Hospital, Taipei, Taiwan R.O.C.
authors.forename: C C
authors.initials: CC
authors.lastname: Shieh
authors.forename: S C
authors.initials: SC
authors.lastname: Chang
authors.forename: C R
authors.initials: CR
authors.lastname: Tzeng
authors.forename: J J
authors.initials: JJ
authors.lastname: Huang
authors.forename: W J
authors.initials: WJ
authors.lastname: Nir
authors.forename: C Y
authors.initials: CY
authors.lastname: Hong
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D012463:Saliva / Q000032:analysis*; D012661:Semen / Q000032:analysis*; D013739:Testosterone / Q000032:analysis* / Q000097:blood
chemical_list: D013739:Testosterone