Biosynthesis of gangliosides from asialogangliosides in rat liver Golgi vesicles.
Biosynthesis of glycolipids GA2, GA1, GM1b, and GD1c was studied in Golgi vesicles isolated from rat liver. Sequential addition of N-acetylgalactosamine, galactose and two sialic acid residues to lactosylceramide led to the endproduct GD1c. Activities of the corresponding glycosyltransferases were shown to be present in isolated Golgi vesicles and their respective kinetic data were determined. The products of each reaction were characterized by their mobility on thin-layer chromatography, by enzymic degradation to their respective precursors, and in case of GM1b by FAB mass spectrometry.
Pohlentz
G
G
Institut für Organische Chemie und Biochemie, Universität Bonn.
Klein
D
D
Schmitz
D
D
Schwarzmann
G
G
Peter-Katalinić
J
J
Sandhoff
K
K
D000818:Animals; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006028:Glycosphingolipids / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D013058:Mass Spectrometry; D051381:Rats
D005732:Gangliosides; D006028:Glycosphingolipids; D006602:Hexosyltransferases; D006026:Glycoside Hydrolases