title: Lectin-gold labeling of glycoconjugates in normal and Brattleboro rat papilla: effect of vasopressin.
abstract: Some reports suggest that the plasma membrane glycocalyx of collecting duct epithelial cells, as well as interstitial glycoconjugates, may be involved in vasopressin action and urinary concentration. In view of this, we have used the lectin-gold technique to map and quantify Helix pomatia lectin (HPL)-binding sites in the inner medulla of kidneys from normal Long-Evans rats, vasopressin-deficient Brattleboro rats, and Brattleboro rats treated for up to 5 wk with exogenous vasopressin. The results show that the labeling of epithelial cell plasma membranes from collecting ducts and thin limbs of Henle is not different between normal and Brattleboro rats, and the labeling is not modified by chronic vasopressin treatment. In contrast, the heavy interstitial labeling seen in normal rats is virtually absent from Brattleboro rats, but it is progressively restored by chronic vasopressin treatment of Brattleboro rats. These results show that vasopressin does not modify HPL-binding glycoconjugates on epithelial cell plasma membranes, but that vasopressin treatment has a major effect on HPL-binding glycoconjugates in the medullary interstitium.
authors.affiliation: Institute of Histology and Embryology, University Medical Centre, Geneva, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Weyer
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: L
authors.initials: L
authors.lastname: Orci
mesh_terms: D000818:Animals; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006046:Gold; D007679:Kidney Medulla / Q000378:metabolism* / Q000648:ultrastructure; D007685:Kidney Tubules, Collecting / Q000378:metabolism / Q000648:ultrastructure; D037102:Lectins; D008138:Loop of Henle / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011910:Rats, Brattleboro / Q000378:metabolism*; D011919:Rats, Inbred Strains; D011922:Rats, Mutant Strains / Q000378:metabolism*; D012016:Reference Values; D014667:Vasopressins / Q000494:pharmacology*
chemical_list: D006023:Glycoproteins; C038095:Helix lectin; D037102:Lectins; D014667:Vasopressins; D006046:Gold