The synthesis of heat-shock proteins after a decrease in translational capacity in Escherichia coli.
Various conditions which decrease translational capacity and enhance the synthesis of ribosomal components were analysed with respect to the synthesis of heat-shock proteins in Escherichia coli: (a) deprivation of streptomycin from a streptomycin-dependent mutant, (b) addition of tetracycline to a partially tetracycline-resistant strain, and (c) nutritional shift-up conditions. In all cases, the rate of synthesis of the heat-shock proteins DnaK, GroEL and C62.5 decreased while the synthesis of ribosomal components increased. Thus inhibition of ribosome formation or a decrease in translational capacity do not induce the stress proteins, but have the opposite effect.
Schnier
J
J
Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, Berlin, FRG.
D001345:Autoradiography; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis / Q000187:drug effects; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance
D006360:Heat-Shock Proteins; D013752:Tetracycline; D013307:Streptomycin