title: Rat hepatocyte lipogenesis and insulin-stimulated lipogenesis: comparison of metabolite effects and methods of measurement.
abstract: This study compared the effects of certain metabolites (either singly or in various combinations) and the methods of measuring lipogenesis (using either 14C-acetate or 3H2O incorporation into lipids) on total lipid synthesis and insulin-stimulated total lipid synthesis in the isolated rat hepatocyte. There were quantitative and qualitative differences between 14C-acetate and 3H2O incorporation into lipids; metabolites acutely affected both lipogenesis and insulin-stimulated lipogenesis with either isotope; and insulin's effect on lipogenesis was greater when measured by 14C-acetate incorporation. It is suggested that a particular choice of incubation media and isotope may inadvertently bias a study of insulin-stimulated lipogenesis and that metabolite supply plays a major role in regulating insulin-stimulated lipogenesis.
authors.affiliation: Department of Clinical Biochemistry, Queen Elizabeth II Medical Centre, Nedlands, Western Australia.
authors.forename: S
authors.initials: S
authors.lastname: Bottomley
authors.forename: P
authors.initials: P
authors.lastname: Garcia-Webb
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D008297:Male; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D011920:Rats, Inbred WF
chemical_list: D000596:Amino Acids; D003470:Culture Media; D007328:Insulin; D007773:Lactates; D008055:Lipids; D011773:Pyruvates; D005947:Glucose