title: Actions of vanadate on vascular tension and sodium pump activity in cat isolated cerebral and femoral arteries.
abstract: 1. The mechanisms involved in the responses induced by sodium vanadate (Va3 VO4) on cat cerebral and femoral arteries were studied. The possibility that these responses were due to Na+, K+-ATPase inhibition was investigated by measuring the effect of vanadate on [3H]-ouabain binding to arterial membrane fractions, K+-induced vasodilatation and ouabain-sensitive 86Rb+ uptake. 2. The vanadium compounds (Na3VO4, VOSO4, VCl3 and O5V3) induced similar, concentration-dependent contractions in each kind of artery, the cerebral vessels being the most sensitive to these compounds. 3. Exposure of the arteries to a low-Na+ (25 mM) solution suppressed the contraction caused by vanadate in femoral but not in cerebral arteries. 4. Vanadate-induced contractions were reduced in Ca2+-free medium but remained unaffected by 3 x 10(-6) M phentolamine, reserpine pretreatment or 3 x 10(-6) M verapamil in both kinds of artery. 5. The addition of 7.5 mM K+ to the arteries immersed in a K+-free solution induced vasodilatation, which was not modified by 10(-3) M vanadate. 6. The consecutive administration of ouabain (10(-4) M) and vanadate (10(-3) M) (or vice versa), or the simultaneous administration of both agents (10(-8) to 10(-3) M) appeared to produce an additive contraction in both types of artery. 7. Vanadate (10(-7) to 10(-3) M) did not displace the [3H]-ouabain binding to arterial membrane fractions of these arteries, whereas 10(-4) M ouabain did. 8. In both kinds of artery, total 86Rb+ uptake was reduced by ouabain (10(-8) to 10(-3) M), in a concentration-dependent manner, whereas it was not modified by vanadate (10(-8)-10(-3) M). 9. These results suggest that vanadate induces contraction in both types of artery by a mechanism unrelated to Na+, K+-ATPase inhibition. Such a mechanism is likely to be related to inhibition of the Ca2-ATPase of the cell membrane and/or the sarcoplasmic reticulum.
authors.affiliation: Departmento de Farmacologia y Terapéutica, Facultad de Medicina, Universidad Autónoma, Madrid, Spain.
authors.forename: C F
authors.initials: CF
authors.lastname: Sánchez-Ferrer
authors.forename: J
authors.initials: J
authors.lastname: Marín
authors.forename: M
authors.initials: M
authors.lastname: Lluch
authors.forename: A
authors.initials: A
authors.lastname: Valverde
authors.forename: M
authors.initials: M
authors.lastname: Salaices
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002415:Cats; D002536:Cerebral Arteries / Q000187:drug effects / Q000294:innervation; D005260:Female; D005263:Femoral Artery / Q000187:drug effects / Q000294:innervation; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D010042:Ouabain / Q000378:metabolism; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D012414:Rubidium Radioisotopes; D014638:Vanadates; D010042:Ouabain; D012964:Sodium