Neurophysin in the large luteal cell of the nonpregnant water buffalo (Bubalus bubalis): immunohistochemical localization.
Light microscopy immunohistochemistry was used to localize neurophysin in the corpus luteum of the mid-luteal phase of the estrous cycle of the water buffalo (Bubalus bubalis). Corpora lutea weighing 0.39-0.65 g from a recent ovulation showed no staining. Corpora lutea identified with the late luteal phase showed only weak evidence of staining. The neurophysin staining was confined to a specific region of large oval-shaped cells (20-30 microns diameter), which had a very eosinophilic cytoplasm. The intense localization of staining to a distinct area of the cytoplasm was previously only observed in the corpus luteum of the cow. Corpora lutea obtained from all quadrants of pregnancy did not stain. Controls in which the neurophysin antiserum was substituted with serum from an unimmunized rabbit (normal rabbit serum) or neurophysin antiserum preabsorbed with bovine oxytocin-associated neurophysin I also did not stain. These data indicate the neurophysin is present in the mature corpus luteum of the nonpregnant water buffalo as it is in other nonpregnant ruminants, the ewe and cow.
Fields
P A
PA
Department of Anatomy, University of South Alabama, Mobile 36688.
Dubois
W
W
Shalash
M R
MR
Fields
M J
MJ
D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D003338:Corpus Luteum / Q000032:analysis*; D004971:Estrus / Q000378:metabolism; D005260:Female; D007124:Immunoenzyme Techniques; D008184:Luteal Cells / Q000032:analysis*; D009481:Neurophysins / Q000032:analysis*; D011247:Pregnancy
D009481:Neurophysins