Differential localization of glycoconjugates having affinity for concanavalin A on the surface of the sperm head in the testis, the epididymis, and the ejaculate of the ram.
The localization of Con A receptors on the surface of the head of ram spermatozoa originating from the rete testis, from three regions of the epididymis, or from the ejaculate was investigated using a gold-Con A labelling technique. Electron microscopic observation revealed three major localizations, each being characteristic of the origin of the spermatozoa: periacrosomal in the rete testis, postacrosomal in the epididymis, on the entire surface of the sperm head in the ejaculate.
Delpech
S
S
I.N.R.A. Reproductive Physiology, Nouzilly, France.
Hamamah
S
S
Pisselet
C
C
Courot
M
M
D000818:Animals; D003208:Concanavalin A; D004542:Ejaculation; D004822:Epididymis; D006001:Glycoconjugates / Q000032:analysis*; D008297:Male; D008358:Mannose / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis*; D008854:Microscopy, Electron; D011952:Receptors, Concanavalin A / Q000032:analysis*; D012756:Sheep; D013077:Sperm Head / Q000276:immunology* / Q000648:ultrastructure; D013094:Spermatozoa / Q000276:immunology*; D013737:Testis
D006001:Glycoconjugates; D008562:Membrane Glycoproteins; D011952:Receptors, Concanavalin A; D003208:Concanavalin A; D008358:Mannose