Detection of human somatic cell structural gene mutations by two-dimensional electrophoresis.
The feasibility of detecting human somatic structural gene mutations by two dimensional electrophoresis has been investigated. A lymphoblastoid cell line was grown as a mass culture in the presence of ethylnitrosourea, after which cells were regrown as single cell clones. A total of 257 polypeptide spots were analyzed in gels derived from 186 clones. Four structural mutations were detected by visual analysis of the gels. Computer analysis of gels corresponding to the mutant clones was also undertaken. At a spot size threshold of 200 spots to be matched using a computer algorithm, all four mutant polypeptides were detected. These results indicate the usefulness of the two-dimensional approach for mutagenesis studies at the protein level.
Hanash
S M
SM
Department of Pediatrics, University of Michigan, Ann Arbor 48109.
Chu
E H
EH
Kuick
R
R
Skolnick
M
M
Neel
J
J
Strahler
J
J
Pivirotto
S
S
Niezgoda
W
W
D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D005821:Genetic Techniques; D006801:Humans; D008214:Lymphocytes; D008432:Mathematical Computing; D009154:Mutation; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification
D011506:Proteins