Investigations on metabolism and carcinogenicity of 1,1,2-trichloroethane.
Two groups of male and female Sprague-Dawley rats (50 animals/group per sex) were treated with either 15.37 or 46.77 mumole of 1,1,2-TCE in DMSO/rat for 2 years. The animals were treated once a week by s.c. injection of test compound in the skin of neck. Two groups of controls received either DMSO or no treatment at all. The incidence of benign mesenchymal and epithelial tumors was not significant when compared with either DMSO-treated or untreated controls. The animals treated with 46.77 mumole 1,1,2-TCE significantly developed sarcomas when compared with the untreated controls. In a further experiment, either 40 mumole or 160 mumole 1,1,2-TCE was injected into male Wistar rats and the metabolites, TdGA and HEMA, were determined in 24-h urine samples. Comparative studies were carried out giving equimolar amounts of chloroethanol and 2-chloroacetaldehyde diethyl acetal. Analysis of the metabolites showed that no detectable HEMA was excreted in urine after treatment of rats with 1,1,2-TCE or chloroethanol. TdGA was excreted in urine much more among chloroacetaldehyde-treated animals than among 1,1,2-TCE- or chloroethanol-treated rats.
Norpoth
K
K
Institute of Hygiene and Occupational Medicine, University Medical Center, Essen University, Federal Republic of Germany.
Heger
M
M
Müller
G
G
Mohtashamipur
E
E
Kemena
A
A
Witting
C
C
D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D004247:DNA / Q000378:metabolism; D005260:Female; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced*; D051381:Rats; D011919:Rats, Inbred Strains; D012513:Sarcoma, Experimental / Q000139:chemically induced; D013864:Thioglycolates / Q000652:urine; D014240:Trichloroethanes / Q000378:metabolism / Q000633:toxicity*
D006843:Hydrocarbons, Chlorinated; D013864:Thioglycolates; D014240:Trichloroethanes; C024567:1,1,2-trichloroethane; C010518:thiodiacetic acid; D004247:DNA; C004656:chloroacetaldehyde; D000079:Acetaldehyde