Actin and calmodulin coexist in the equatorial segment of ejaculated boar sperm.
The direct overlay technique with iodinated calmodulin on boar sperm fractions evidenced a strong bond in the 45,000 molecular weight, which is the region recognized by anti-actin antibodies. This result and the close codistribution of the two staining patterns for calmodulin and actin with immunofluorescence and immunoelectronmicroscopy suggest a possible interaction between calmodulin and actin in boar sperm.
Camatini
M
M
Dipartimento di Biologia, Università di Milano, Italy.
Casale
A
A
D000199:Actins / Q000032:analysis*; D000818:Animals; D002147:Calmodulin / Q000032:analysis*; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D004542:Ejaculation; D005455:Fluorescent Antibody Technique; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D013094:Spermatozoa / Q000032:analysis* / Q000166:cytology / Q000648:ultrastructure; D013552:Swine
D000199:Actins; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins