The effects of cannabinoids and cannabispiro compounds on Escherichia coli adhesion to tissue culture cells and on leukocyte functions in vitro.
delta 9-Tetrahydrocannabinol, cannabidiol, cannabidiolic acid, tetrahydrocannabidiolic acid, cannabispirol, acetylcannabispirol, cannabispirone, and cannabispirenone in a low concentration did not affect the adhesion of Escherichia coli on cultured HEp-2 cells. Cannabinoids at 10(-6) M increased the chemiluminescence of human polymorphonuclear leukocytes, while the cannabispiro compounds failed to enhance the oxidative burst of leukocytes. In lymphocyte and granulocyte function tests (E- and EA-rosette formation, blast transformation of T-lymphocytes in the presence of phytohaemagglutinin and concanavalin-A, ADCC and phagocytosis) all compounds displayed immunosuppressive effect at 1.5 X 10(-5) M. Tetrahydrocannabidiolic acid exerted the weakest immunosuppression on human leukocyte functions.
Molnár
J
J
Institute of Microbiology, University Medical School, Szeged, Hungary.
Petri
I
I
Berek
I
I
Shoyama
Y
Y
Nishioka
I
I
D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D001422:Bacterial Adhesion / Q000187:drug effects*; D002186:Cannabinoids / Q000494:pharmacology*; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007165:Immunosuppression Therapy; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D008163:Luminescent Measurements; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D012397:Rosette Formation; D013141:Spiro Compounds / Q000494:pharmacology*
D002186:Cannabinoids; D013141:Spiro Compounds