title: Gas chromatographic analysis of bromophenylmercapturic acids in urine.
abstract: A gas chromatographic assay specific for the analysis of bromophenylmercapturic acids in urine is described. The mercapturic acids are first hydrolyzed to form bromothiophenols, which are acetylated, extracted into dichloromethane, and analyzed by gas chromatography with a flame ionization detector. Using samples from rats and mice treated with bromobenzene, the authors make a comparison between this specific method and a nonspecific assay for the determination of total thioethers in urines.
authors.affiliation: Département de médecine, Faculté de médecine, Université de Montréal, Québec, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Tardif
authors.forename: D
authors.initials: D
authors.lastname: Talbot
authors.forename: M
authors.initials: M
authors.lastname: Gérin
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D000818:Animals; D002849:Chromatography, Gas; D003124:Colorimetry; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008968:Molecular Conformation; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards
chemical_list: D007202:Indicators and Reagents; C055229:S-(2-bromophenyl)mercapturic acid; C055230:S-(3-bromophenyl)mercapturic acid; C053003:S-phenyl-N-acetylcysteine; C055228:S-(4-bromophenyl)mercapturic acid; D000111:Acetylcysteine