title: A resonance Raman study of the binding of ethanol and methanol to ferrihemoglobin.
abstract: The interactions of ethanol and methanol with ferrihemoglobin were examined using resonance Raman spectroscopy. After binding either alcohol, the low-frequency resonance Raman spectra of human ferrihemoglobin are almost identical to the unperturbed spectrum except for shifts in the 309 cm-1 band to higher frequency by as much as 8 cm-1. The ethanol-induced shift is greater than that with methanol even though complex formation was less for ethanol than methanol. The spectral changes imply a site-specific, similar binding of these alcohols to ferrihemoglobin which may involve steric interactions. Possible assignments of the 309 cm-1 band to structural features as well as potential mechanisms of the alcohol-induced spectral changes are discussed.
authors.affiliation: Department of Chemistry, Purdue University School of Science, Indiana University-Purdue University at Indianapolis 46223.
authors.forename: B B
authors.initials: BB
authors.lastname: Muhoberac
authors.forename: J A
authors.initials: JA
authors.lastname: Shelnutt
authors.forename: M R
authors.initials: MR
authors.lastname: Ondrias
mesh_terms: D000818:Animals; D001665:Binding Sites; D000431:Ethanol / Q000378:metabolism*; D006736:Horses; D006801:Humans; D000432:Methanol / Q000378:metabolism*; D008706:Methemoglobin / Q000378:metabolism*; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman
chemical_list: D000431:Ethanol; D008706:Methemoglobin; D000432:Methanol