title: Strain-specific serum antibody responses in infants undergoing primary infection with respiratory syncytial virus.
abstract: We sought to characterize the human respiratory syncytial virus (RSV) subgroup-specific antibody response to primary infection with RSV. RSV isolates from 43 infants and young children were typed as either subgroup A (Long strain-like) or B (18537 strain-like) based on reactivity with monoclonal antibodies. Acute-phase or preinfection and convalescent-phase sera were collected from the 43 subjects and tested in an enzyme-linked immunosorbent assay using purified F and G glycoproteins from RSV subgroups A and B and by neutralization assay against both subgroups of RSV. Twenty-six individuals had rises in titer of antibody to F glycoprotein, 27 to G glycoprotein, and 28 had rises in titer of neutralizing antibody. The antibody responses to homologous and heterologous F glycoproteins were not significantly different. In contrast, homologous vs. heterologous antibody responses to G glycoprotein were significantly different, with 7.3% relatedness between the G glycoproteins of subgroups A and B. Analysis of neutralizing antibody responses revealed 31% relatedness.
authors.affiliation: Division of Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892.
authors.forename: R M
authors.initials: RM
authors.lastname: Hendry
authors.forename: J C
authors.initials: JC
authors.lastname: Burns
authors.forename: E E
authors.initials: EE
authors.lastname: Walsh
authors.forename: B S
authors.initials: BS
authors.lastname: Graham
authors.forename: P F
authors.initials: PF
authors.lastname: Wright
authors.forename: V G
authors.initials: VG
authors.lastname: Hemming
authors.forename: W J
authors.initials: WJ
authors.lastname: Rodriguez
authors.forename: H W
authors.initials: HW
authors.lastname: Kim
authors.forename: G A
authors.initials: GA
authors.lastname: Prince
authors.forename: K
authors.initials: K
authors.lastname: McIntosh
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D003429:Cross Reactions; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007223:Infant; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D010253:Respirovirus Infections / Q000276:immunology*; D013045:Species Specificity; D013997:Time Factors; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D007074:Immunoglobulin G; D014764:Viral Proteins