Periodic acid Schiff--p phenylenediamine staining of glycogen in chondrocytes. A new combination which improves both cellular detail and glycogen identification.
This study reports a method whereby glycogen is identified in the chondrocytes of the secondary center of ossification prior to mineralization. The use of new fuchsin rather than basic fuchsin on one micron Spurr sections of femoral head cartilage fixed with potassium ferrocyanide-reduced osmium produced excellent identification of glycogen and when followed by p phenylenediamine, intensified cellular detail.
Shepard
N
N
E.M. Unit, Shriners Hospital, Montreal, Quebec, Canada.
Mitchell
N
N
D000818:Animals; D002356:Cartilage / Q000032:analysis* / Q000648:ultrastructure; D006003:Glycogen / Q000032:analysis*; D006651:Histocytochemistry; D010503:Periodic Acid-Schiff Reaction; D010655:Phenylenediamines; D051381:Rats
D010655:Phenylenediamines; D006003:Glycogen; C029728:4-phenylenediamine