Cloning and sequencing of cDNA that encodes goat growth hormone.
The cDNA that encodes goat growth hormone (gGH) was isolated from a goat pituitary cDNA library. The cDNA, about 880 base pairs long, had a coding sequence, 5'- and 3'-untranslated regions and a poly(A) chain. The cDNA could encode a polypeptide of 217 amino acids. The amino acid sequence homology between gGH and the sequences of bovine GH, rat GH and human GH was 99, 83 and 66%, respectively. By Northern blot hybridization, we found that the possible gGH gene is transcribed in the goat pituitary.
Yamano
Y
Y
Department of Agricultural Chemistry, Kyoto University, Japan.
Oyabayashi
K
K
Okuno
M
M
Yato
M
M
Kioka
N
N
Manabe
E
E
Hashi
H
H
Sakai
H
H
Komano
T
T
Utsumi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D006041:Goats / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010902:Pituitary Gland / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D012333:RNA, Messenger; D013006:Growth Hormone; D004247:DNA