title: Identification of ADP in the iron-sulfur flavoprotein trimethylamine dehydrogenase.
abstract: Analysis of the 2.4-A resolution electron density map of trimethylamine dehydrogenase has revealed the unexpected presence of one molecule of ADP/subunit. This binding has been confirmed chemically. The binding site is located at the analogous position of the ADP moiety of FAD in glutathione reductase, the FAD and NADPH binding domains of which resemble two of the domains of trimethylamine dehydrogenase. Comparison of the environments of the ADP moieties in the two proteins indicates that 32 residues in 6 peptides are in equivalent positions with a root mean square deviation for C alpha positions of 1.11 A. Twelve of these amino acids are identical, based on the electron density-derived "x-ray" sequence of trimethylamine dehydrogenase. Detailed analysis of the environment of the ADP moiety indicates that most of the conserved residues are not in direct contact with the cofactor. Some of them probably represent the "fingerprint" of the beta alpha beta binding fold found in dinucleotide binding proteins, but the remaining conserved residues may indicate a closer evolutionary relationship between these two proteins.
authors.affiliation: Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: L W
authors.initials: LW
authors.lastname: Lim
authors.forename: F S
authors.initials: FS
authors.lastname: Mathews
authors.forename: D J
authors.initials: DJ
authors.lastname: Steenkamp
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis* / Q000378:metabolism; D001665:Binding Sites; D002855:Chromatography, Thin Layer; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006860:Hydrogen Bonding; D009249:NADP / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000032:analysis* / Q000378:metabolism; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005182:Flavin-Adenine Dinucleotide; D009249:NADP; D000244:Adenosine Diphosphate; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase; D005980:Glutathione Reductase