Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae. IV. Influence of DNA replication and excision repair on REV2 dependent UV-mutagenesis and repair.
A double mutant being thermoconditionally defective in mutation induction as well as in repair of pre-lethal UV-induced DNA damage (rev2ts) and deficient in excision repair (rad3-2) was studied in temperature-shift experiments. The influence of inhibitors of DNA replication (hydroxyurea, aphidicolin) was determined. Additionally, an analysis of the dose-response pattern of mutation induction ("mutation kinetics") at several ochre alleles was carried out. It was concluded that the UV-inducible REV2 dependent mutagenic repair process is not induced in excision-deficient cells. In excision-deficient cells, REV2 dependent mutation fixation is slow and mostly post-replicative though not dependent on DNA replication. The REV2 mediated mutagenic process could be separated from the repair function.
Siede
W
W
Gesellschaft für Strahlen- und Umweltforschung, Institut für Strahlenbiologie, Neuherberg, Federal Republic of Germany.
Eckardt
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F
D004260:DNA Repair / Q000528:radiation effects; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005800:Genes, Fungal; D006918:Hydroxyurea / Q000494:pharmacology; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D013696:Temperature; D014466:Ultraviolet Rays
D006918:Hydroxyurea