Human immune response to allergens of house dust mite, Dermatophagoides pteronyssinus. III. Cross-reactivity of bystander idiotopes on allergen-specific IgE antibodies.
Rabbits were inoculated with affinity-purified human antibodies to the common house dust mite Dermatophagoides pteronyssinus (DPT) to produce anti-idiotypic (Id) antibodies. The Id carried by human IgE antibodies were studied in a radioimmunoassay in which specific anti-DPT IgE were first insolubilized on rabbit F(ab')2 fragments against human Fc epsilon. In a single individual specific anti-DPT IgE antibodies shared bystander idiotopes (i.e. idiotopes located outside the antigen-binding site) with IgG antibodies of the same specificity, since the adsorption on insolubilized anti-DPT IgG almost completely eliminated the binding of anti-Id antibodies to IgE. This differed from our previous observations (J.-M. R. Saint-Remy et al., Eur. J. Immunol. 1986. 16: 575) wherein distinct paratope-associated idiotopes were identified on IgG and IgE anti-DPT antibodies. The frequency of cross-reactions between idiotopes of anti-DPT IgE antibodies observed in ten unrelated individuals was less than 10% for bystander idiotopes. This finding confirmed the private character of the above idiotopes that we found earlier on anti-DPT IgG antibodies. However, one anti-Id antibody preparation was found to react with bystander idiotopes of anti-DPT IgE of most individuals, thereby suggesting the existence of a recurrent idiotope. The low frequency of inter-individual bystander idiotope cross-reactivity contrasted with the public character of paratope-associated idiotopes.
Saint-Remy
J M
JM
Institute of Cellular and Molecular Pathology, Université Catholique de Louvain, Brussels, Belgium.
Lebecque
S J
SJ
Lebrun
P M
PM
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D039741:Antigens, Dermatophagoides; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D004391:Dust; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D008925:Mites / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity
D000485:Allergens; D039741:Antigens, Dermatophagoides; D004391:Dust; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007073:Immunoglobulin E