A turbidometric assay for measuring proteins in culture media.
An immunoturbidometric assay was developed for the measurement of proteins in culture fluids of hepatocytes. The assay is simple to perform and avoids the biohazards associated with radioimmunoassays. The limit of detection of this assay exemplified by hemopexin and transferrin is 5 ng/ml protein. This degree of sensitivity is attained by incorporating into the procedure the addition of polyethylene glycol to enhance formation of primary antigen-antibody complexes and of a second antibody to further increase the immune complex size, which favors the ratio of specific to background light scattering.
Wojciechowicz
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D
New York Hospital-Cornell University Medical College, Department of Pediatrics 10021.
De Falco
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M
Muller-Eberhard
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U
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D002460:Cell Line; D003470:Culture Media / Q000032:analysis*; D006466:Hemopexin; D007118:Immunoassay / Q000379:methods*; D007202:Indicators and Reagents; D008114:Liver Neoplasms, Experimental; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D014168:Transferrin
D000936:Antigen-Antibody Complex; D003470:Culture Media; D007202:Indicators and Reagents; D011506:Proteins; D014168:Transferrin; D006466:Hemopexin