Primary structure of nonspecific crossreacting antigen (NCA), a member of carcinoembryonic antigen (CEA) gene family, deduced from cDNA sequence.
A cDNA containing the entire coding region for a member of carcinoembryonic antigen (CEA) gene family has been cloned from cDNA library of HLC-1 cells by immunochemical screening with the antibody specific to nonspecific crossreacting antigen (NCA). The cDNA encodes a precursor form of a polypeptide consisting of a 34-residue signal sequence, a 108-residue N-terminal (N-) domain, a 178-residue domain (NCA-I domain) and a 24-residue domain rich in hydrophobic amino acids (M-domain). Each domain has a distinct but homologous amino acid sequence to that of the corresponding domain of CEA. Unlike the coding sequences, the 3'-untranslated sequences differ markedly in the NCA and CEA cDNAs facilitating the preparation of probes that will discriminate between nucleotide sequences for CEA and NCA.
Tawaragi
Y
Y
Suntory Institute for Biomedical Research, Osaka, Japan.
Oikawa
S
S
Matsuoka
Y
Y
Kosaki
G
G
Nakazato
H
H
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics; D015815:Cell Adhesion Molecules; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007118:Immunoassay; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D012334:RNA, Neoplasm; D004247:DNA