Renotropic activity of lutropin: direct stimulation of DNA synthesis of cultured rat renal cortical cells.
Two differently purified ovine lutropin (LH) preparations were studied to see if they stimulated [3H] thymidine incorporation into cultured rat renal cortical cells. Both preparations showed a dose-dependent (0.11-1 ng/ml), time-dependent (peaking at 18 h), serum-dependent (7.5% of fetal calf serum or 10% castrated-hypo-physectomized rat serum) renotropic effect. Ovine TSH and FSH failed to mimic this specific, renotropic effect. We concluded that LH directly stimulates renal DNA synthesis in cooperation with other serum factor's.
Nomura
K
K
Department of Medicine, Tokyo Women's Medical College, Japan.
Horiba
N
N
Sato
Y
Y
Ujihara
M
M
Demura
H
H
Shizume
K
K
D000818:Animals; D002369:Castration; D002417:Cattle; D004247:DNA / Q000096:biosynthesis*; D005312:Fetal Blood; D007016:Hypophysectomy; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007986:Luteinizing Hormone; D004247:DNA