title: Brugia malayi: arachidonic acid uptake into lipid bodies of adult parasites.
abstract: Lipid bodies are non-membrane bound intracellular organelles, which have been recognized morphologically in a diversity of mammalian and nonmammalian cells, but are of uncertain function. In mammalian cells, in addition to serving as a storage site of cholesterol and triglyceride, lipid bodies can be a repository of esterified arachidonic acid. Adult worms of the human filarial parasite Brugia malayi have been found to esterify exogenous [3H]arachidonic acid into parasite phospholipids and neutral lipids. Electron microscopic autoradiography demonstrated that [3H]arachidonate was preferentially incorporated into filarial lipid bodies. The dominant incorporation of arachidonate into lipid bodies of a nematode establishes that lipid bodies are a site of arachidonic acid accumulation in nonmammalian, as well as mammalian, cells.
authors.affiliation: Department of Medicine, Beth Israel Hospital, Boston, Massachusetts.
authors.forename: D L
authors.initials: DL
authors.lastname: Longworth
authors.forename: R A
authors.initials: RA
authors.lastname: Monahan-Earley
authors.forename: A M
authors.initials: AM
authors.lastname: Dvorak
authors.forename: P F
authors.initials: PF
authors.lastname: Weller
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001345:Autoradiography; D002009:Brugia / Q000378:metabolism* / Q000648:ultrastructure; D004952:Esters; D005260:Female; D050356:Lipid Metabolism; D008297:Male; D008854:Microscopy, Electron; D009940:Organoids / Q000378:metabolism / Q000648:ultrastructure; D010743:Phospholipids / Q000378:metabolism
chemical_list: D001095:Arachidonic Acids; D004952:Esters; D010743:Phospholipids