Hormone-sensitive lipase in brown adipose tissue: identification and effect of cold exposure.
Hormone-sensitive lipase (HSL) in brown adipose tissue from mice was identified through immunoprecipitation with a polyclonal antibody (anti-HSL) towards rat white fat HSL and Western blotting. An 82 kDa polypeptide, slightly smaller than the rat white fat HSL 84 kDa subunit, was detected and its identity as HSL verified by inhibition properties. The HSL concentration per g tissue was several-fold higher in the mouse brown adipose tissue than in the rat white adipose tissue, but the specific activities per mg protein were similar. Cold-exposure (4 degrees C) of the mice for 24 h approximately doubled the HSL concentration but this increase parallelled the overall protein increase and did not reflect a specific effect on the HSL.
Holm
C
C
Department of Medical and Physiological Chemistry, University of Lund, Sweden.
Fredrikson
G
G
Cannon
B
B
Belfrage
P
P
D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D003080:Cold Temperature; D004075:Diglycerides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D007531:Isoflurophate / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D012969:Sodium Fluoride / Q000494:pharmacology; D002787:Sterol Esterase / Q000032:analysis* / Q000276:immunology; D014280:Triglycerides / Q000378:metabolism
D000936:Antigen-Antibody Complex; D004075:Diglycerides; D014280:Triglycerides; D007531:Isoflurophate; D012969:Sodium Fluoride; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase