Enzymic properties of intestinal aminopeptidase P: a new continuous assay.
A continuous photometric assay of aminopeptidase P activity was developed which is based on a coupled enzymic assay with the substrate Gly-Pro-Pro-pNA and DPP IV as auxiliary enzyme. This assay was used to evaluate the kinetic parameters and inhibitory profile of intestinal brush border aminopeptidase P.
Lasch
J
J
Institute of Biochemistry, Medical Faculty, Martin-Luther-University Halle, GDR.
Koelsch
R
R
Steinmetzer
T
T
Neumann
U
U
Demuth
H U
HU
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D007202:Indicators and Reagents; D007700:Kinetics; D009842:Oligopeptides / Q000138:chemical synthesis; D013053:Spectrophotometry / Q000379:methods; D013379:Substrate Specificity
D007202:Indicators and Reagents; D009842:Oligopeptides; D000626:Aminopeptidases; C022750:X-Pro aminopeptidase