In vitro adhesion of K88ab-, K88ac- and K88ad-positive Escherichia coli to intestinal villi, to buccal cells and to erythrocytes of weaned piglets.
The phenotype of 21 weaned piglets, concerning adhesion of Escherichia coli possessing K88ab, K88ac or K88ad fimbriae to pig cells, was determined in an in vitro assay. Comparison was made with adhesion of these three K88 variant strains to buccal mucosal epithelial cells and to erythrocytes (haemagglutination) in the same piglets. Whereas adhesion of the three K88 variant strains to intestinal villi was piglet specific, buccal cell adhesion (BCA) and haemagglutination (HA) were not. The K88ab strain was weakly adhesive or non-adhesive in the BCA and negative in the HA test. K88ac strains consistently gave negative and K88ad consistently gave positive results in both assays. After washing the bacteria with phosphate-buffered saline, the K88ab strain revealed a positive HA test. Neither the BCA, nor HA test can be used to determine the pig intestinal adhesive phenotype.
Cox
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E
University of Antwerp, State University Centre of Antwerp, Belgium.
Houvenaghel
A
A
D000818:Animals; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D004912:Erythrocytes / Q000382:microbiology*; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D029968:Escherichia coli Proteins; D005260:Female; D036781:Fimbriae Proteins; D006386:Hemagglutination Tests; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000648:ultrastructure; D008871:Microvilli / Q000382:microbiology; D009061:Mouth Mucosa / Q000382:microbiology*; D010641:Phenotype; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D036781:Fimbriae Proteins