A microelectrometric titration method for measurement of total intracellular Cl- concentration.
A microelectrometric titration method is described to measure picomole amounts of Cl- present in solutions at micromolar concentrations. This method was used to measure total intracellular chloride concentration ([Cl-]i) in leukocytes. Through the use of submicroliter samples, [Cl-] can be measured in the range of 5-500 microM. There is no measurable interference from other ions normally present in the cell and no intracellular ion is falsely measured as Cl-. [Cl-]i determined by the conventional coulometric titration and the new microelectrometric titration method was the same. Among the commonly used substituting anions, thiocyanate was the only one falsely measured as Cl- and should be avoided in experiments using this method. Because only picomole amounts of Cl- are required, measurements of intracellular pH and total concentrations of other intracellular ions can be done in the same lysate of as few as 5 X 10(5) cells. This feature should make it possible to study the time course of changes in [Cl-]i together with measurements of other intracellular ions following various physiological or experimental maneuvers.
Bomsztyk
K
K
Division of Nephrology, University of Washington, Seattle 98195.
Calalb
M B
MB
Smith
L
L
Stanton
T H
TH
D000818:Animals; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D051379:Mice; D008839:Microelectrodes; D009994:Osmolar Concentration; D051381:Rats
D002712:Chlorides