Investigations on the binding of erythrogenic toxin A of Streptococcus pyogenes on human peripheral blood lymphocytes. I. Light and electron microscopical demonstration of cell surface receptors using colloidal gold-labelled toxin.
Receptors for erythrogenic toxin A (ETA) of Streptococcus pyogenes (strain NY-5) were demonstrated on human peripheral blood lymphocytes by binding of ETA-gold conjugates to the cell surface. The specificity of the binding was proved in control experiments. The dark-red granules observed by light microscopy on unfixed cells were revealed by transmission electron microscopy to be patches of gold particles. By light microscopy on samples from 48 blood donors a mean value of 34 +/- 13% of ETA-receptor bearing lymphocytes was ascertained. The predominant part of the cells exhibited only a weak or moderate labelling. Transmission electron microscopy of prefixed cells revealed an attachment of single gold particles distributed over the whole cell surface. Counts of gold particles on serial sections yielded depending on prefixation values of 50-600 receptors/cell (1% glutaraldehyde) and 1000-7000 receptors/cell (0.26% glutaraldehyde), respectively. ETA cloned in Streptococcus sanguis as well as the toxoid of ETA exhibited a comparable binding as ETA (NY-5).
Scriba
S
S
Zentralinstitut für Mikrobiologie und experimentelle Therapie Jena der Akademie der Wissenschaften der DDR.
Wagner
B
B
Wagner
M
M
Gerlach
D
D
Köhler
W
W
D001426:Bacterial Proteins; D003102:Colloids; D005098:Exotoxins / Q000378:metabolism*; D006046:Gold; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D008565:Membrane Proteins; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011971:Receptors, Immunologic / Q000032:analysis* / Q000378:metabolism; D013297:Streptococcus pyogenes
D001426:Bacterial Proteins; D003102:Colloids; D005098:Exotoxins; D008565:Membrane Proteins; D011971:Receptors, Immunologic; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin; C049791:erythrogenic toxin A receptor; D006046:Gold