A galactose specific agglutinin from the hemolymph of the snail Achatina fulica: purification and characterization.
A galactose specific agglutinin has been detected in the hemolymph of the snail Achatina fulica fulica Bowdich. The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purified agglutinin has a native molecular weight of 210,000 and comprises of non covalently linked identical subunits of molecular weight 15,000. The pI of the agglutinin was found to be 8.1. The activity of the agglutinin is independent of divalent cations. The inhibition of hemagglutination data reveals that 2-deoxy-D-galactose is the most potent inhibitor of the agglutinin followed by galactose and lactose.
Mitra
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D
Indian Institute of Chemical Biology, Ja davpur, Calcutta.
Sarkar
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M
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D037161:Galectins; D006385:Hemagglutination Inhibition Tests; D006388:Hemagglutinins / Q000302:isolation & purification*; D006458:Hemolymph / Q000276:immunology*; D007526:Isoelectric Point; D008970:Molecular Weight; D011487:Protein Conformation; D012908:Snails / Q000276:immunology*
D037161:Galectins; D006388:Hemagglutinins