Metabolism of chloral hydrate in the anoxic perfused liver.
The metabolism of chloral hydrate (CH) under anoxic conditions was investigated in the non-recirculating, hemoglobin-free liver perfusion system. CH uptake in the anoxic liver decreased to about 80% of that in the oxygen-supplied liver. The reduction of CH to trichloroethanol (TCE) increased and the oxidation of CH to trichloroacetic acid (TCA) decreased. The TCE/TCA ratio increased; however, the total trichloro compounds, that is TCE and TCA, were not significantly altered by anoxia. Though approximate 14% of the CH infused into the oxygen-supplied liver was changed to substances other than TCE or TCA, the unknown part was a very small portion in the anoxic liver. The decrease in CH uptake, by the anoxic liver, is thought to be equivalent to the decrease of the unknown metabolites. The TCE/TCA ratio under anoxia was also altered by pyruvate or lactate infusion.
Kawamoto
T
T
Department of Public Health, Yamaguchi University School of Medicine, Ube, Japan.
Hobara
T
T
Aoki
T
T
Kobayashi
H
H
Iwamoto
S
S
Sakai
T
T
Takano
T
T
Miyazaki
Y
Y
Imamura
A
A
Ogino
K
K
D000818:Animals; D002697:Chloral Hydrate / Q000378:metabolism*; D007773:Lactates / Q000494:pharmacology; D019344:Lactic Acid; D008099:Liver / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000032:analysis*; D010477:Perfusion; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains
D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D002697:Chloral Hydrate; D019289:Pyruvic Acid; D010100:Oxygen