title: The biological importance of each amino acid residue of the troponin I inhibitory sequence 104-115 in the interaction with troponin C and tropomyosin-actin.
abstract: To systematically evaluate the contribution of each amino acid residue of the troponin I (TnI) inhibitory region (104-115), 14 synthetic analogs were synthesized by the solid-phase method. The analogs consisted of either single glycine or multiglycine replacements. The importance of the substituted amino acid(s) was determined from the extent of inhibition of the acto-S1 ATPase activity and the strength of binding to a troponin C (TnC) high pressure liquid chromatography affinity column of each synthetic analog. Every residue of the TnI sequence (104-115) is necessary to achieve maximum inhibition of the ATPase activity. However, the analogs quantitatively differed in the amount of inhibition induced. The TnI analogs bound less tightly to the TnC affinity column than the native synthetic peptide indicating that all residues in the TnI sequence contribute to the binding of TnC in the presence of Mg2+ or Ca2+. In the presence of Ca2+, there is a definite increase in the strength of the interaction between most analogs and TnC. This is accompanied with a shift toward a more specific interaction with the C terminus of the TnI inhibitory sequence.
authors.affiliation: Medical Research Council Group in Protein Structure and Function, University of Alberta, Edmonton, Canada.
authors.forename: J E
authors.initials: JE
authors.lastname: Van Eyk
authors.forename: R S
authors.initials: RS
authors.lastname: Hodges
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D011817:Rabbits; D014335:Tropomyosin / Q000378:metabolism*; D014336:Troponin / Q000032:analysis* / Q000378:metabolism*; D019209:Troponin C; D019210:Troponin I
chemical_list: D000199:Actins; D010446:Peptide Fragments; D014335:Tropomyosin; D014336:Troponin; D019209:Troponin C; D019210:Troponin I; C033025:actin subfragments