title: CFU-GEMM correlate with neutrophil and platelet recovery in patients receiving autologous marrow transplantation after high-dose melphalan chemotherapy.
abstract: CFU-GEMM, multipotent progenitor cells, give rise to erythroid, granulocyte-macrophage and megakaryocytic cells. We have examined the utility of this assay for predicting hematologic recovery in patients treated with high-dose chemotherapy and autologous marrow rescue with cryopreserved marrow. In marrow samples from 50 patients CFU-E, BFU-E and CFU-GEMM significantly decreased following cryopreservation. The median value for CFU-E declined from 132,659 to 10,648, BFU-E decreased from 36,112 to 3345 and CFU-GEMM decreased from 3242 to 260 colonies per ml of marrow frozen. Once cryopreserved, the number of CFU-E, BFU-E and CFU-GEMM remained stable with prolonged storage in liquid nitrogen. In 48 patients uniformly treated with high-dose melphalan (180 mg/m2) and rescued with cryopreserved autologous marrow, univariate analyses showed that the time to platelet recovery (greater than 50 x 10(9)/l) was correlated with the number of CFU-E, BFU-E and CFU-GEMM infused (p less than 0.05). The time to neutrophil recovery was only correlated with the number of BFU-E and CFU-GEMM infused (p less than 0.01). However, by multivariate analyses, only the number of CFU-GEMM, and not CFU-E and BFU-E, infused correlated both with the time to neutrophil and platelet recovery. These data indicate that the CFU-GEMM assay may be useful for determining the repopulating ability of cryopreserved bone marrow.
authors.affiliation: Research Service, Veterans' Administration Hospital, San Antonio, TX 78284.
authors.forename: G D
authors.initials: GD
authors.lastname: Roodman
authors.forename: C F
authors.initials: CF
authors.lastname: LeMaistre
authors.forename: G M
authors.initials: GM
authors.lastname: Clark
authors.forename: C P
authors.initials: CP
authors.lastname: Page
authors.forename: T F
authors.initials: TF
authors.lastname: Newcomb
authors.forename: W A
authors.initials: WA
authors.lastname: Knight
mesh_terms: D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D005615:Freezing; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D008264:Macrophages / Q000166:cytology; D008533:Megakaryocytes / Q000166:cytology; D008558:Melphalan / Q000008:administration & dosage*; D009369:Neoplasms / Q000628:therapy*; D013997:Time Factors; D014021:Tissue Preservation; D014182:Transplantation, Autologous
chemical_list: D008558:Melphalan