Lysis of a lung carcinoma by poly I:C-induced natural killer cells is independent of the expression of class I histocompatibility antigens.
Cells from the line 1 murine carcinoma express little if any H-2d when grown in normal medium. These cells are susceptible to splenic cell populations with NK activity, stimulated by prior injection of poly I:C, but are not lysed by NK-deficient splenocytes from homozygous beige mice treated with anti-asialo GM1. Incubation of line 1 cells in medium containing DMSO leads to a dramatic stimulation of H-2d expression but no change in lytic susceptibility to splenic NK cells. Transfection of H-2Dp into line 1 leads to a constitutive and DMSO-inducible expression of H-2Dp at functionally significant levels, but this expression appears to have no influence on NK cytolytic susceptibility.
Dennert
G
G
University of Southern California Comprehensive Cancer Center, Los Angeles 90033.
Landon
C
C
Lord
E M
EM
Bahler
D W
DW
Frelinger
J G
JG
D000818:Animals; D002277:Carcinoma / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011070:Poly I-C / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D006183:H-2 Antigens; D011070:Poly I-C