title: Protective effect of glucan against experimentally induced staphylococcal mastitis in ewes.
abstract: Glucan, an immunostimulant, was evaluated for its ability to modify a staphylococcal mammary challenge in ewes. Glucan was administered subcutaneously to ewes prior to lactation or during lactation, and all ewes, including a control group, were subsequently challenged intramammarily with Staphylococcus haemolyticus 40 days after the mean lambing date. The glucan treatment was shown to modify the staphylococcal mammary infection as the milk bacterial counts from all of the glucan-treated groups were significantly reduced compared to controls. For the glucan-treated groups, the highest mean somatic cell counts were recorded 1 day post-challenge, while for the control group, the mean cell count rose more gradually to peak by 3 days post-challenge. Glucan did not increase serum lysozyme levels or blood neutrophil bactericidal activity. However, there was a negative correlation between the bactericidal activity of blood neutrophils collected from the glucan-treated ewes prior to challenge and their mean milk bacterial counts post-challenge. Glucan was observed to stimulate ovine mammary macrophages in vitro, while the addition of zymosan or opsonised killed Staphylococcus aureus to macrophage cultures had no effect. These studies indicate that, in sheep, glucan can enhance some elements of the immune system against staphylococcal infections.
authors.affiliation: Wallaceville Animal Research Centre, Ministry of Agriculture and Fisheries, Upper Hutt, New Zealand.
authors.forename: B M
authors.initials: BM
authors.lastname: Buddle
authors.forename: H D
authors.initials: HD
authors.lastname: Pulford
authors.forename: M
authors.initials: M
authors.lastname: Ralston
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005936:Glucans / Q000627:therapeutic use*; D008264:Macrophages / Q000276:immunology / Q000502:physiology; D008413:Mastitis / Q000276:immunology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*; D013203:Staphylococcal Infections / Q000276:immunology / Q000662:veterinary*; D013210:Staphylococcus / Q000276:immunology / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D005936:Glucans