[A histofluorimetric method of evaluating the insulin level of pancreatic islets].
Up to now results of the histochemical luminiscent reaction to insulin in pancreatic islet beta cells (the pseudoisocyanin test) were assessed by a visual method which permitted establishing the difference only in substantial variations of fluorescence intensity. The authors employed a quantitative method for assessment of the results of the test using a microfluorimetric attachment designed by them. They also provided data on the measurement of the insulin content in pancreatic islet beta cells of rats in different experimental exposures using this method.
Meĭramov
G G
GG
Tusupbekova
G T
GT
Meĭramova
R G
RG
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D005470:Fluorometry; D066298:In Vitro Techniques; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000032:analysis* / Q000473:pathology; D008856:Microscopy, Fluorescence; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin
Gistofliuorimetricheskiĭ metod otsenki soderzhaniia insulina v pankreaticheskikh ostrovkakh.