title: In vivo 31P nuclear magnetic resonance spectroscopy of subcutaneous 9L gliosarcoma: effects of tumor growth and treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea on tumor bioenergetics and histology.
abstract: In vivo 31P nuclear magnetic resonance spectroscopy was used to examine the bioenergetics of the rat 9L gliosarcoma during untreated growth and in response to chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea. Tumor growth was associated with a decline in the phosphocreatine and nucleoside triphosphate resonances, consistent with an increase in tumor hypoxia during untreated growth. Following chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea (10 mg/kg), tumor levels of phosphocreatine and nucleoside triphosphate rebounded while the level of inorganic phosphate in the tumor declined. Histological comparison of treated and untreated tumor sections 4 days posttreatment showed that the treated tumor had a lower proportion of necrotic cells, a higher proportion of viable cells, and a 5-fold higher level of interstitial space than the control tumor.
authors.affiliation: Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: R G
authors.initials: RG
authors.lastname: Steen
authors.forename: R J
authors.initials: RJ
authors.lastname: Tamargo
authors.forename: K A
authors.initials: KA
authors.lastname: McGovern
authors.forename: S S
authors.initials: SS
authors.lastname: Rajan
authors.forename: H
authors.initials: H
authors.lastname: Brem
authors.forename: J P
authors.initials: JP
authors.lastname: Wehrle
authors.forename: J D
authors.initials: JD
authors.lastname: Glickson
mesh_terms: D000818:Animals; D002330:Carmustine / Q000627:therapeutic use; D004734:Energy Metabolism; D005110:Extracellular Space / Q000473:pathology; D005910:Glioma / Q000098:blood supply / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D009336:Necrosis; D009711:Nucleotides / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats
chemical_list: D009711:Nucleotides; D010725:Phosphocreatine; D002330:Carmustine