title: Two distinct succinate thiokinases in both bloodstream and procyclic forms of Trypanosoma brucei.
abstract: Two succinate thiokinase activities specific for either adenine or guanine nucleotides have been found in Trypanosoma brucei. Key glycolytic and citric acid cycle enzymes were measured to show repression of glycolysis and derepression of the citric acid cycle in the procyclic form, relative to the bloodstream form. A marked rise in adenine-linked succinate thiokinase activity accompanied a rise in activity of citric acid cycle enzymes. However, guanine-linked succinate thiokinase was found to increase only slightly in activity. These results implicate the adenine-linked enzyme as an essential component of the citric acid cycle, whereas the guanine-linked enzyme appears to be under separate control. This communication also reports for the first time the occurrence of citrate synthase activity in the bloodstream (long slender) form of T. brucei.
authors.affiliation: Department of Biochemistry, University of Bath, U.K.
authors.forename: T M
authors.initials: TM
authors.lastname: Jenkins
authors.forename: R
authors.initials: R
authors.lastname: Eisenthal
authors.forename: P D
authors.initials: PD
authors.lastname: Weitzman
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D002952:Citric Acid Cycle; D003066:Coenzyme A Ligases / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006031:Glycosylation; D006150:Guanine Nucleotides / Q000378:metabolism; D013379:Substrate Specificity; D013389:Succinate-CoA Ligases / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000254:growth & development
chemical_list: D000227:Adenine Nucleotides; D006150:Guanine Nucleotides; D002950:Citrate (si)-Synthase; D003066:Coenzyme A Ligases; D013389:Succinate-CoA Ligases; D005947:Glucose