Latent infection with feline syncytial virus of cell cultures prepared from the kidneys of new-born kittens.
Feline syncytial virus infection was detected in 4 out of 30 secondary or tertiary cultures of kidney cells from new-born kittens. Such infection was not detected in any primary cell culture. The syncytia-forming virus was readily transmissible by infected cells. One strain was transmissible to heterologous (bovine and canine) secondary cells, but did not infect MDBK and PK-15 cell lines. Based on biochemical, untrastructural and serological characteristics, the virus was designated a member of the subfamily Spumavirinae of the family Retroviridae. As endogenous infection of primary cell cultures may not be detectable by cytopathic effect, it is suggested that only well-controlled secondary cat cells or cell lines should be used in work with feline viruses.
Kükedi
A
A
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.
Bartha
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A
Nagy
B
B
D000818:Animals; D000831:Animals, Newborn; D002415:Cats; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D007668:Kidney / Q000382:microbiology*; D009500:Neutralization Tests; D012190:Retroviridae / Q000254:growth & development* / Q000302:isolation & purification