Enzyme immunoassay for detection of antigen in acute Plasmodium falciparum malaria.
A monoclonal antibody specific for an epitope of a 50 kDa Plasmodium falciparum antigen was used in an enzyme immunoassay for detection of the corresponding exo-antigen in culture supernatant and in the sera of 31 patients suffering from acute malaria. The assay was specific for Plasmodium falciparum and did not appear to be strain restricted. A parasitaemia level below 0.001% could be detected.
Fortier
B
B
Unité 42 INSERM, Villeneuve d'Ascq, France.
Delplace
P
P
Dubremetz
J F
JF
Ajana
F
F
Vernes
A
A
D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008288:Malaria / Q000175:diagnosis*; D010963:Plasmodium falciparum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan