Murine c-myc retroviruses alter the growth requirements of myeloid cell lines.
Recombinant retroviruses encoding a murine c-myc gene were utilised to explore the consequences of constitutive c-myc expression for interleukin 3-dependent murine myeloid cell lines. The c-myc virus-infected cells exhibited a diminished requirement for growth factor and serum when grown in soft agar, but no factor-independent cells could be isolated and the cells were not tumorigenic. Thus deregulated expression of a c-myc gene at physiological levels apparently renders myeloid cells more responsive to growth factors but cannot abrogate this requirement or render the cells malignant.
Cory
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S
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Bernard
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O
Bowtell
D
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Schrader
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S
Schrader
J W
JW
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002460:Cell Line; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D007377:Interleukin-3 / Q000502:physiology*; D008407:Mast Cells / Q000502:physiology; D008533:Megakaryocytes / Q000502:physiology; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D012190:Retroviridae / Q000235:genetics
D004274:DNA, Recombinant; D007377:Interleukin-3; D011518:Proto-Oncogene Proteins