title: Extended storage of single-donor apheresis platelets in CLX blood bags: effect of storage on platelet morphology, viability and in vitro function.
abstract: A CLX (Cutter Laboratories, Berkeley, Calif.) bag system was evaluated for storage of single-donor apheresis platelets collected with the Haemonetics V-50 blood processor. Concentrates (n = 21) containing 3.9-5.2 x 10(11) platelets in 292 (+/- 41.8) ml were stored in two 1-liter bags for 7 days at 22 degrees C. pH was well maintained, declining from an initial pH of 7.0 (+/- 0.04) to 6.92 (+/- 0.20) after 7 days. Platelet morphology, response to a hypotonic stimulus and aggregation induced by paired agonists (epinephrine and ADP, or collagen) were also well-preserved. Concentrates with a wide variation of platelet yields (2.0 greater than or equal to 5.2 x 10(11), n = 43) also maintained pH (6.96 +/- 0.26), morphology and aggregation when stored for 7 days. All platelet concentrates (n = 64) were sterile at collection. Single-donor apheresis platelets may be stored in this bag system for up to 7 days.
authors.affiliation: Center for Blood Research, Boston, Mass.
authors.forename: D M
authors.initials: DM
authors.lastname: Kenney
authors.forename: J J
authors.initials: JJ
authors.lastname: Peterson
authors.forename: J W
authors.initials: JW
authors.lastname: Smith
mesh_terms: D001781:Blood Component Removal; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology; D001793:Blood Preservation / Q000295:instrumentation*; D002470:Cell Survival; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008855:Microscopy, Electron, Scanning; D010974:Platelet Aggregation; D010976:Platelet Count; D010983:Plateletpheresis