Monoclonal antibodies against calcitonin. Characterization and application in light and electron microscopy immunocytochemistry.
Twenty-seven monoclonal antibodies (MAbs) to synthetic human calcitonin (CT) were characterized for their reactivities with human CT peptide fragments by dot-blot analysis on nitrocellulose paper. Most of the antibodies bound to the C-terminus and fewer to the mid-region of CT. We have studied thyroid tissue specimens from several animal species after fixation in paraformaldehyde-, glutaraldehyde- or picric acid-containing mixtures and cryostat sectioning or embedment in paraffin or plastic (Epon 812 or Lowicryl 4KM) using this panel of MAbs. The site of antigen-antibody reaction was revealed either by immunoperoxidase, immunoalkaline phosphatase or by silver-enhanced immunogold staining methods. All MAbs were able to localize CT in human, rat and mouse thyroid C cells. Nineteen MAbs recognizing synthetic salmon CT and synthetic [Asu1,7]-eel CT by dot-blot, reacted with chicken ultimobranchial body C cells. One MAb recognizing native porcine CT by dot-blot, stained C cells in hog thyroid. Immunopositivity was confined to the cytoplasm and ultrastructural immunogold labelling demonstrated that cytoplasmic secretory granules were stained. Surgical specimens from human medullary thyroid carcinoma were also analysed for the presence of CT and a variable number of positive cells was found. Furthermore, Congo red-positive areas were shown to react with the MAbs. All conventional staining and immunoabsorption controls were negative. Hence, these MAbs may be suitable for use in routine immunopathological diagnosis of CT-producing tumors and for immunocytochemical localization of the three major CT variants in different animal species.
Scopsi
L
L
Unit of Histochemistry, University Institute of Pathological Anatomy, Copenhagen, Denmark.
Arias
J
J
Houen
G
G
Racchetti
G
G
Fossati
G L
GL
Galante
Y M
YM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002116:Calcitonin / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D006801:Humans; D007118:Immunoassay; D007150:Immunohistochemistry; D051379:Mice; D008853:Microscopy; D008854:Microscopy, Electron; D051381:Rats; D013961:Thyroid Gland / Q000032:analysis*; D013964:Thyroid Neoplasms / Q000032:analysis*
D000911:Antibodies, Monoclonal; D002116:Calcitonin