title: Release of oligosaccharides possessing reducing-end N-acetylgalactosamine from mucus glycoprotein in Streptomyces sp. OH-11242 culture medium through action of endo-type glycosidase.
abstract: A crude enzyme preparation from a culture medium of Streptomyces sp. OH-11242 contained endo-alpha-N-acetylgalactosaminidase activity. The activity could be induced by the addition of purified porcine gastric mucin to the culture medium. Oligosaccharides corresponding to approximately 2-14 glucose units were detected in the culture medium and also in an incubated reaction mixture of crude enzyme preparation and mucus glycoprotein. The resulting product with N-acetylgalactosamine at the reducing terminal implied the presence of a new type of endo-glycosidase liberating not only Gal beta 1-3GalNAc but also other larger oligosaccharides by hydrolysis of the O-glycosidic linkage between GalNAc and Ser (Thr).
authors.affiliation: Department of Biochemistry, School of Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iwase
authors.forename: I
authors.initials: I
authors.lastname: Ishii
authors.forename: K
authors.initials: K
authors.lastname: Ishihara
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Omura
authors.forename: K
authors.initials: K
authors.lastname: Hotta
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005753:Gastric Mucosa; D006023:Glycoproteins / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism*; D006868:Hydrolysis; D009077:Mucins / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology*; D013552:Swine; D048809:alpha-N-Acetylgalactosaminidase
chemical_list: D003470:Culture Media; D006023:Glycoproteins; D009077:Mucins; D009844:Oligosaccharides; C047874:gastric mucus glycoproteins; D006596:Hexosaminidases; D048809:alpha-N-Acetylgalactosaminidase