title: Characterization and comparison of two newly established Epstein-Barr virus-negative lymphoma B-cell lines. Surface markers, growth characteristics, cytogenetics, and transplantability.
abstract: Two Epstein-Barr virus (EBV)-negative lymphoma B-cell lines, HBL-1 and HBL-2, were established from a pleural effusion and a lymph node biopsy of two patients with diffuse large cell lymphoma. HBL-1 and HBL-2 showed the characteristics of activated B-cells in B-cell lineage, as did original lymphoma cells. Chromosome analyses revealed that HBL-1 exhibiting 14q+ marker-positive lymphoid cancer showed a new subclass of 14q32 translocation resulting from a translocation between chromosomes 14 and 16, which had been masked in a complex translocation involving five chromosomes, and that HBL-2 had a 14q+ marker chromosome, the result of an 11;14 translocation [t(11;14)(q13;32)]. Successful heterotransplantation into athymic nude mice demonstrated tumorigenicity of HBL-1 and HBL-2. The transplantability and tumor growth rate of HBL-2 were higher and more rapid than those of HBL-1. HBL-1 and HBL-2 appear useful for facilitating therapeutic investigations as well as immunologic and oncogenic studies in B-cell lymphomas.
authors.affiliation: Department of Pathology, Fukushima Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: Y
authors.initials: Y
authors.lastname: Nozawa
authors.forename: H
authors.initials: H
authors.lastname: Wakasa
authors.forename: H
authors.initials: H
authors.lastname: Ohno
authors.forename: S
authors.initials: S
authors.lastname: Fukuhara
mesh_terms: D000368:Aged; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D002051:Burkitt Lymphoma / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D002460:Cell Line; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D014178:Translocation, Genetic
chemical_list: D000954:Antigens, Surface