Effect of mouse interferon alpha/beta on the expression of H-2 (class I) antigens and on the levels of 2'-5' oligoadenylate synthetase activity in interferon-sensitive and interferon-resistant Friend leukemia cell tumors in mice.
DBA/2 mice were injected intraperitoneally (i.p.) with interferon-sensitive 745 or interferon-resistant 3C1-8 Friend erythroleukemia cells (FLC) and then injected i.p. with mouse interferon alpha/beta. Interferon enhanced the expression of histocompatibility (H-2) antigens on individual 745 FLC within the peritoneum, but did not alter the expression of H-2 antigens on individual 3C1-8 FLC. Likewise, interferon treatment resulted in an increase in the level of 2'-5' oligo-adenylate (2-5A) synthetase activity in 745 FLC, but did not affect the level of activity in 3C1-8 FLC. These results provide evidence that the phenotype of interferon sensitivity or resistance of FLC does not change within the peritoneum. An incidental finding was that the basal level of 2-5A synthetase activity of in vivo passaged 745 cells was greater than that of 3C1-8 FLC. The finding that injection of mice bearing 745 FLC with antibody to mouse interferon alpha/beta reduced the level of 2-5A synthetase activity in these cells, but did not alter the level of 2-5A activity in 3C1-8 FLC, suggests that endogenous interferon in the peritoneum may have been the responsible factor.
Locardi
C
C
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
Belardelli
F
F
Federico
M
M
Romeo
G
G
Affabris
E
E
Gresser
I
I
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005622:Friend murine leukemia virus; D006183:H-2 Antigens / Q000276:immunology*; D007370:Interferon Type I / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D008811:Mice, Inbred DBA
D000906:Antibodies; D006183:H-2 Antigens; D007370:Interferon Type I; D015088:2',5'-Oligoadenylate Synthetase