title: Polewards chromosome movement driven by microtubule depolymerization in vitro.
abstract: We constructed complexes between isolated chromosomes and microtubules made from purified tubulin to study the movement of chromosomes towards the 'minus' end of microtubules in vitro, a process analogous to the movement of chromosomes towards the pole of the spindle at anaphase of mitosis. Our results show that the energy for this movement is derived solely from microtubule depolymerization, and indicate that anaphase movement of chromosomes is both powered and regulated by microtubule depolymerization at the kinetochore.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
authors.forename: D E
authors.initials: DE
authors.lastname: Koshland
authors.forename: T J
authors.initials: TJ
authors.lastname: Mitchison
authors.forename: M W
authors.initials: MW
authors.lastname: Kirschner
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000705:Anaphase; D001710:Biotin; D002875:Chromosomes / Q000502:physiology*; D003432:Cross-Linking Reagents; D004734:Energy Metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D008870:Microtubules / Q000502:physiology*; D009068:Movement; D011108:Polymers; D008941:Spindle Apparatus / Q000502:physiology; D014404:Tubulin / Q000502:physiology
chemical_list: D003432:Cross-Linking Reagents; D011108:Polymers; D014404:Tubulin; D001710:Biotin; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate