title: Agrobacterium-mediated infectivity of cloned digitaria streak virus DNA.
abstract: A monomeric clone of double-stranded DNA synthesized in vitro DNA of the geminivirus Digitaria streak (DSV) was subcloned as a tandem dimeric unit into a binary vector of Agrobacterium tumefaciens, creating a plasmid pDS2. Inoculation of digitaria sanguinalis with A. tumefaciens carrying pDS2 resulted in viral infection. The symptoms, virus particles, and DNA forms obtained were indistinguishable from those of a natural DSV infection of D. sanguinalis. Inoculations have also induced infections in Zea mays and Avena sativa. The sequence of the Agrobacterium-mediated infectious clone of DSV has been determined.
authors.affiliation: John Innes Institute, AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Donson
authors.forename: H V
authors.initials: HV
authors.lastname: Gunn
authors.forename: C J
authors.initials: CJ
authors.lastname: Woolston
authors.forename: M S
authors.initials: MS
authors.lastname: Pinner
authors.forename: M I
authors.initials: MI
authors.lastname: Boulton
authors.forename: P M
authors.initials: PM
authors.lastname: Mullineaux
authors.forename: J W
authors.initials: JW
authors.lastname: Davies
mesh_terms: D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors; D010942:Plant Viruses / Q000235:genetics* / Q000502:physiology; D010944:Plants / Q000382:microbiology*; D012231:Rhizobium / Q000235:genetics
chemical_list: D004279:DNA, Viral