title: Phospholipase A2 hydrolysis of membrane phospholipids causes structural alteration of the nicotinic acetylcholine receptor.
abstract: Thermal perturbation techniques have been used to probe structural alteration of the nicotinic acetylcholine receptor as a function of perturbations of its native membrane environment. Differential scanning calorimetry and a technique involving heat inactivation of the alpha-bungarotoxin-binding sites on the receptor protein reveal that there is a profound destabilization of the acetylcholine receptor structure when receptor-containing membranes are exposed to phospholipase A2. The characteristic calorimetric transition assigned to irreversible denaturation of the receptor protein and the heat inactivation profile of alpha-bungarotoxin-binding sites are shifted to lower temperatures by approx. 7 and 5 C degrees, respectively, upon exposure to phospholipase A2 at a phospholipase/neurotoxin binding site molar ratio of about 1:100. The effects of phospholipase A2 on receptor structure can be (i) reversed by using bovine serum albumin as a scavenger of phospholipase hydrolysis products of membrane phospholipids, and (ii) stimulated by incorporation into the membranes of free, polyunsaturated fatty acids. In particular, linolenic acid (18:3(n-3] causes detectable destabilization of the alpha-bungarotoxin binding sites on the receptor at free fatty acid/receptor molar ratios as low as 10:1. Furthermore, alteration of receptor structure by added phospholipase occurs very rapidly, which is consistent with the observation of rapid in situ phospholipase A2 hydrolysis of membrane phospholipids, particularly highly unsaturated phosphatidylethanolamine and phosphatidylserine. Based on previously published data on the inhibition of acetylcholine receptor cation-gating activity caused by the presence of either phospholipase A2 or free fatty acids (Andreasen T.J. and McNamee M.G. (1980) Biochemistry 19, 4719), we interpret our data as indicative of a correlation between structural and functional alterations of the membrane-bound acetylcholine receptor induced by phospholipase A2 hydrolysis products.
authors.affiliation: Department of Biology (School of Sciences), University of the Balearic Islands, Palma de Mallorca, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Villar
authors.forename: A
authors.initials: A
authors.lastname: Artigues
authors.forename: J A
authors.initials: JA
authors.lastname: Ferragut
authors.forename: J M
authors.initials: JM
authors.lastname: Gonzalez-Ros
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002462:Cell Membrane / Q000378:metabolism; D004546:Elapid Venoms; D004557:Electric Organ / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008563:Membrane Lipids / Q000502:physiology*; D010740:Phospholipases / Q000494:pharmacology*; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D010743:Phospholipids / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000378:metabolism*; D014101:Torpedo
chemical_list: D004546:Elapid Venoms; D008563:Membrane Lipids; D010743:Phospholipids; D011978:Receptors, Nicotinic; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2