Increased levels of fibroblast growth factor-like activity in urine from patients with bladder or kidney cancer.
Growth factor activity was partially purified from human renal tumors and a human bladder cancer cell line by heparin-Sepharose chromatography. This activity stimulated bovine capillary endothelial cell proliferation and DNA synthesis in BALB/c 3T3 cells. Partially purified growth factor preparations from these tumors contained a protein with an approximate molecular weight of 17,000 which was recognized by a polyclonal antiserum raised against a peptide fragment of basic fibroblast growth factor (FGF). This growth factor activity appears to be related to basic fibroblast growth factor. Measurement of FGF-like activity in 50 urine samples from 32 adult males showed that 55% (6 of 11) of the urine samples from patients with bladder cancer and 100% (7 of 7) of the urine samples from patients with kidney cancer contained activity equivalent to more than 20 ng of basic FGF/h of urine production. In contrast, only 6% (2 of 32) of the urine samples from controls, patients with a benign disease, or patients with a history of bladder or kidney cancer contained this level of growth factor activity. These results suggest that patients with bladder or kidney cancer release an FGF-like factor into urine which may be used as a marker for these tumors.
Chodak
G W
GW
Department of Surgery/Urology, University of Chicago, Illinois 60637.
Hospelhorn
V
V
Judge
S M
SM
Mayforth
R
R
Koeppen
H
H
Sasse
J
J
D000230:Adenocarcinoma / Q000652:urine; D043925:Angiogenesis Inducing Agents / Q000032:analysis; D002277:Carcinoma / Q000652:urine; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors / Q000032:analysis / Q000276:immunology / Q000652:urine*; D006133:Growth Substances / Q000652:urine*; D006493:Heparin / Q000652:urine*; D006801:Humans; D007680:Kidney Neoplasms / Q000652:urine*; D008297:Male; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000652:urine*
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D006493:Heparin