Identification of colony-forming activities of various hemopoietic cells in the serum-free culture medium of a human embryo fibroblast cell strain.
Ralph et al. reported that human cell lines for the production of colony stimulating factor cannot secrete the factor in serum-free media (Blood 68, 633-639, 1986). However, we found various colony-forming activities to be present in the serum-free culture medium of a human embryo fibroblast cell strain (YH-1). When a soy bean trypsin inhibitor was added to the serum-free media, semisolid cultures of mouse and human bone marrow cells exhibited a considerable degree of colony formation by macrophages and to a lesser extent by other cells. A difference in apparent molecular weights in macrophage colony-forming activities by YH-1 cells from serum-free and serum-containing cultures was observed. The physiological significance of these findings is discussed in terms of regulation of hemopoietic cell differentiation by human embryo fibroblasts.
Okai
Y
Y
Tokyo Research Laboratory, Kyowa Hakko Kogyo Co. Ltd., Japan.
Nakahata
T
T
D001769:Blood; D001853:Bone Marrow / Q000196:embryology; D001854:Bone Marrow Cells; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000166:cytology; D009504:Neutrophils / Q000166:cytology
D003470:Culture Media