[Role of Pseudomonas maltophilia and "Pseudomonas like bacteria group Va" in the etiology of Crohn disease].
In 1976, Parent and Mitchell isolated cell-wall defective bacteria of the species Pseudomonas maltophilia and Pseudomonas like bacteria group Va from resected bowel tissue of 8 consecutively operated patients with Crohn's disease. The control group, which included patients with ulcerative colitis, was negative in this respect. The isolated strains were available for our own investigations. New Zealand white rabbits were inoculated with killed bacteria to produce specific antisera. Frozen sections of affected tissue from 6 consecutively operated Crohn patients were incubated with the various antisera and investigated by indirect immunofluorescence. No positive reactions were demonstrable in repeated tests with different dilutions of antisera. Our results do not support the hypothesis that bacteria of the species Pseudomonas maltophilia and Pseudomonas like bacteria group Va are an etiologic factor in Crohn's disease.
Purrmann
J
J
Medizinische Klinik der Universität Düsseldorf, Abteilung für Gastroenterologie.
Stelzner
M
M
Brunner
H
H
Borchard
F
F
Ehms
H
H
Welters
B
B
Miller
B
B
Strohmeyer
G
G
D001431:Bacteriological Techniques; D003424:Crohn Disease / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008854:Microscopy, Electron; D011549:Pseudomonas / Q000648:ultrastructure; D011552:Pseudomonas Infections / Q000382:microbiology*
Untersuchungen zur Rolle von Pseudomonas maltophilia und "Pseudomonas like bacteria group Va" in der Atiologie des Morbus Crohn.