title: HPLC separation of corticosterone metabolites and its use in following corticosterone metabolism and transport in the isolated rat hepatocyte.
abstract: Incubating isolated rat hepatocytes with tritium labelled and unlabelled corticosterone at 37 degrees C resulted in the rapid appearance of at least nine corticosterone metabolites. A quick, relatively easy, and quantitative high performance liquid chromatographic (HPLC) method was used to separate these metabolites and follow their rate of appearance both intra- and extracellularly. We found different intra- and extracellular amounts of each metabolite at a particular time and this suggested that some metabolites were more available for transport than others.
authors.affiliation: Department of Clinical Biochemistry, Queen Elizabeth II Medical Centre, Nedlands, Perth, Western Australia.
authors.forename: S
authors.initials: S
authors.lastname: Bottomley
authors.forename: C
authors.initials: C
authors.lastname: Lovell-Smith
authors.forename: P
authors.initials: P
authors.lastname: Garcia-Webb
mesh_terms: D000818:Animals; D001692:Biological Transport; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000302:isolation & purification / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011920:Rats, Inbred WF; D014316:Tritium
chemical_list: D014316:Tritium; D003345:Corticosterone