title: Cell attachment and fibrinogen binding properties of platelet and endothelial cell thrombospondin are not affected by structural differences in the 70 and 18 kDa protease-resistant domains.
abstract: Structural differences between platelet and endothelial cell thrombospondin (TBSP) were found in two protease-resistant domains (70 and 18 kDa). The 70 kDa fragment is involved in the binding of TBSP to fibrinogen and the 18 kDa fragment in the attachment to various cultured cells. Despite these structural differences, platelet and endothelial cell TBSP bound with the same affinity to fibrinogen and mediated the attachment of smooth muscle cells but not of endothelial cells.
authors.affiliation: INSERM U63, Laboratoire d'Hémobiologie, Faculté de Médecine A. Carrel, Lyon, France.
authors.forename: P
authors.initials: P
authors.lastname: Clezardin
authors.forename: M C
authors.initials: MC
authors.lastname: Bourdillon
authors.forename: N R
authors.initials: NR
authors.lastname: Hunter
authors.forename: J L
authors.initials: JL
authors.lastname: McGregor
mesh_terms: D001792:Blood Platelets / Q000032:analysis*; D002448:Cell Adhesion; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000032:analysis* / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism; D013820:Thermolysin / Q000378:metabolism; D019699:Thrombospondins
chemical_list: D006023:Glycoproteins; D010446:Peptide Fragments; D019699:Thrombospondins; D005340:Fibrinogen; D013820:Thermolysin