title: Proteolytic activity of human tumor cell lines deriving from bronchial squamous cell carcinoma, pulmonary metastasis of rhabdomyosarcoma and pleural metastasis of mesothelioma.
abstract: The proteolytic activities of human tumor cell lines deriving from bronchial squamous cell carcinoma, a lung metastasis of an embryonal rhabdomyosarcoma and a pleural mesothelioma were measured by use of chromogenic substrates. N-acetyl-alanine aminopeptidase activity, plasminogen activator activity, H-D-Ile-Pro-Arg-p-NA splitting activity as well as plasmin-like activity, cathepsin G-like activity and plasma-kallikrein-like activity were found in cell lysates. The enzymatic activity of N-acetyl-alanine aminopeptidase, plasminogen activator and H-D-Ile-Pro-Arg-p-NA splitting activity changed during culturing. Plasminogen activator and H-D-Ile-Pro-Arg-p-NA splitting activity decreased to very low values, whereas N-acetyl-alanine aminopeptidase activity leveled at 1 x 10(-5) mU/cell. Unlike other proteolytic activities, plasminogen activator was released into the medium. Plasminogen activator activity could be measured in culture medium which contained no fetal calf serum.
authors.affiliation: Thoraxklinik Heidelberg-Rohrbach, Heidelberg, FRG.
authors.forename: O L
authors.initials: OL
authors.lastname: Schoenberger
authors.forename: S
authors.initials: S
authors.lastname: Beikirch
authors.forename: G
authors.initials: G
authors.lastname: Trefz
authors.forename: P
authors.initials: P
authors.lastname: Drings
authors.forename: W
authors.initials: W
authors.lastname: Ebert
mesh_terms: D002283:Carcinoma, Bronchogenic / Q000201:enzymology; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000556:secondary*; D008654:Mesothelioma / Q000201:enzymology; D009842:Oligopeptides / Q000378:metabolism; D010447:Peptide Hydrolases / Q000032:analysis / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism; D012208:Rhabdomyosarcoma / Q000201:enzymology; D014407:Tumor Cells, Cultured / Q000201:enzymology*
chemical_list: D009842:Oligopeptides; C041319:isoleucyl-prolyl-arginine-4-nitroanilide; D010447:Peptide Hydrolases; D010960:Plasminogen Activators