Inhibition of mutagenicity by glycyrrhiza extract and glycyrrhizin.
The effects of Glycyrrhiza extract and one of its components, glycyrrhizin, on the mutagenicities of several mutagens were investigated by means of a modification of the Ames' test. Both inhibited the mutagenicities of 3-amino-1,4-dimethyl-5 H-pyrido[4,3-b]-indole (Trp-P-1) and 3-amino-1-methyl-5 H-pyrido[4,3-b]indole. Since the Glycyrrhiza extract and glycyrrhizin inhibited the mutagenicity of activated Trp-P-1, it was clear that their inhibitory effects were not due to inhibition of the enzyme activity of the S9 fraction. Both Glycyrrhiza extract and glycyrrhizin also inhibited the mutagenicities of benzo[a]pyrene, 3-methylcholanthrene, 2-naphthylamine, 2-amino-6-methyldipyrido[1,2-a:3',2'-d]-imidazole, dimethylnitrosoamine and dimethylaminoazobenzene. The mutagenicity of 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) was inhibited by the Glycyrrhiza extract but not by glycyrrhizin. This suggested that a component different from glycyrrhizin, present in the Glycyrrhiza extract, inhibited the mutagenicity of AF-2.
Tanaka
M
M
School of Pharmaceutical Science, Toho University, Chiba, Japan.
Mano
N
N
Akazai
E
E
Narui
Y
Y
Kato
F
F
Koyama
Y
Y
D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006035:Glycyrrhiza; D019695:Glycyrrhizic Acid; D009152:Mutagenicity Tests; D009154:Mutation; D010946:Plants, Medicinal; D012486:Salmonella typhimurium / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid