Progression of rearrangements at T cell receptor beta and gamma gene loci during athymic differentiation of bone marrow cells in vitro.
A previously described in vitro system that supports T cell differentiation from bone marrow (BM) precursors was analyzed for T cell receptor gene rearrangement and expression. Cultured populations from days (d.) 0, 3, 6, and 12-13 were fused with BW5147. Only hybridomas from d.6 and d.12-13 bore BM-derived rearrangements. The rearrangement complexity was higher on d.12 than d.6 in that patterns consistent with D beta 1-J beta 1 and D beta 2-germ-line configurations decreased while V-D-J beta 2 and V gamma 2-J gamma 1 joining became dominant. Northern blots of d.13 BM cultures revealed gamma, alpha, and beta (1.0 and 1.3 kb) transcripts. Parallels between patterns in BM cultures and thymus ontogeny were recognized.
Hurwitz
J L
JL
Basel Institute for Immunology, Switzerland.
Samaridis
J
J
Pelkonen
J
J
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002459:Cell Fusion; D002478:Cells, Cultured; D005796:Genes; D006412:Hematopoietic Stem Cells / Q000166:cytology; D046911:Macromolecular Substances; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell