Establishment and characterization of a human urinary bladder carcinoma cell line (TSGH-8301).
A cell line derived from a well-differentiated human transitional cell carcinoma of the urinary bladder, designated TSGH-8301, was established in vitro. The cultured epithelioid cells exhibited monolayer growth and loss of contact inhibition. The tumorigenicity of TSGH-8301 had been shown by growth in soft agar and tumor induction in athymic nude mice. A reverse ratio of lactate dehydrogenase (LHD) isoenzyme in the cell line and nude mouse-grown tumors was seen predominantly with LDH-V. Chromosomal analysis revealed a heterodiploid stem line with a modal number of 50. Sera of urinary bladder cancer patients reacted with membrane antigens of the TSGH-8301 cells, suggesting the existence of tumor-associated antigens in the cells. In vitro chemosensitivity tests of these cells may provide data valuable in the selection of proper anticancer drugs for the TSGH-8301 donor patient.
Yeh
M Y
MY
Department of Surgery, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Yü
D S
DS
Chen
S C
SC
Lin
M S
MS
Chang
S Y
SY
Ma
C P
CP
Han
S H
SH
D000818:Animals; D002277:Carcinoma / Q000473:pathology* / Q000648:ultrastructure; D002869:Chromosome Aberrations; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000473:pathology* / Q000648:ultrastructure
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase