title: Identification of T-kinin-Leu(T-kinin-containing peptide) released from T-kininogen by cathepsin D of granulomatous tissues in rats.
abstract: Acid proteinases of granulomatous tissues in rats with carrageenin-induced inflammation released kinin from T-kininogen. By column chromatography on pepstatin-Sepharose 4B, two types of acid proteinase seems to be responsible for kinin release. One of the acid proteinase was identified as cathepsin D from SDS-polyacrylamide gel electrophoresis and Western-blot analysis, using anti-rat liver cathepsin D IgG. Cathepsin D alone could not release T-kinin, but T-kinin-containing peptides. The T-kinin-containing peptides were separated into two peptides by reverse-phase high-performance liquid chromatography. From determination of its amino acid composition and its immunoreactivity toward anti-bradykinin antiserum, one of the T-kinin-containing peptides was identified as T-kinin-Leu.
authors.affiliation: Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
authors.forename: W
authors.initials: W
authors.lastname: Sakamoto
authors.forename: F
authors.initials: F
authors.lastname: Satoh
authors.forename: S
authors.initials: S
authors.lastname: Nagasawa
authors.forename: H
authors.initials: H
authors.lastname: Handa
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002351:Carrageenan; D002402:Cathepsin D / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006099:Granuloma / Q000209:etiology / Q000378:metabolism*; D007704:Kininogens / Q000378:metabolism*; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects
chemical_list: D000596:Amino Acids; D007704:Kininogens; C054879:bradykinin, Leu-Ile-Ser-; C039065:T-kinin; D002351:Carrageenan; D002402:Cathepsin D; D001920:Bradykinin