Cloning of human androgen receptor complementary DNA and localization to the X chromosome.
The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using a consensus nucleotide sequence from the DNA-binding domain of the family of nuclear receptors. The AR gene was localized on the human X chromosome between the centromere and q13. Cloned complementary DNA, selected with an AR-specific oligonucleotide probe, was expressed in monkey kidney (COS) cells and yielded a high-affinity androgen-binding protein with steroid-binding specificity corresponding to that of native AR. A predominant messenger RNA species of 9.6 kilobases was identified in human, rat, and mouse tissues known to contain AR and was undetectable in tissues lacking AR androgen-binding activity, including kidney and liver from androgen-insensitive mice. The deduced amino acid sequence of AR within the DNA-binding domain has highest sequence identity with the progesterone receptor.
Lubahn
D B
DB
Department of Pediatrics, University of North Carolina, Chapel Hill 27599.
Joseph
D R
DR
Sullivan
P M
PM
Willard
H F
HF
French
F S
FS
Wilson
E M
EM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003062:Codon; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D011944:Receptors, Androgen / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
D003062:Codon; D011944:Receptors, Androgen