[Antibiotics and hematopoiesis: toxicity of beta-lactam on in vitro granulopoiesis].
The neutropenia induced by beta-lactam antibiotics conduct us to study the in vitro effect of these antibiotics on human granulopoiesis. We use a semi-solid medium (agar) with placenta conditioned medium as support of granulo-monocytic progenitors growth. We tested the inhibitory effect of prolonged exposure of bone marrow cells to several classes of beta-lactam antibiotics. We found a dose-dependent inhibition in all cases, more pronounced for cephalosporins than for ureido-penicillins. A short (1 hour) exposure was less toxic and could explain the rare occurrence of neutropenia in patients treated with these drugs.
Marie
J P
JP
Laboratoire de Culture Cellulaire, Hôtel-Dieu, Paris.
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006098:Granulocytes / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D008297:Male; D013997:Time Factors; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams
Antibiotiques et hématopoïèse: toxicité des bêta-lactamines sur la granulopoïèse in vitro.