Interaction of neocuproine, 1,10-phenanthroline and 2,2'-dipyridyl with human ceruloplasmin.
1. Neocuproine binding to ceruloplasmin markedly increases the chlorpromazine-ceruloplasmin-catalyzed oxidation of NADH. 2. 1,10-Phenanthroline and 2,2'-dipyridyl inhibit neocuproine activation in a competitive manner. 3. The order of enzyme chelator complex stability was: phenanthroline greater than dipyridyl greater than neocuproine.
Løvstad
R A
RA
Institute of Medical Biochemistry, University of Oslo, Norway.
D015082:2,2'-Dipyridyl / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000378:metabolism*; D002570:Ceruloplasmin / Q000378:metabolism*; D002746:Chlorpromazine / Q000378:metabolism; D006801:Humans; D007502:Iron Chelating Agents / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000378:metabolism*; D011485:Protein Binding; D011725:Pyridines / Q000378:metabolism*
D000970:Antineoplastic Agents; D007502:Iron Chelating Agents; D010618:Phenanthrolines; D011725:Pyridines; D009243:NAD; D015082:2,2'-Dipyridyl; C002701:neocuproine; D002570:Ceruloplasmin; D002746:Chlorpromazine; C025205:1,10-phenanthroline