Spontaneous regeneration of older dystrophic muscle does not reflect its regenerative capacity.
Young dystrophic (dy) murine muscle is capable of "spontaneous" regeneration (i.e., regeneration in the absence of external trauma); however, by the time the mice are 8 weeks old, this regeneration ceases. It has been suggested that the cessation of regeneration in dystrophic muscle may be due to exhaustion of the mitotic capability of myosatellite cells during the early stages of the disease. To test this hypothesis, orthotopic transplantation of bupivacaine treated, whole extensor digitorum longus muscles has been performed on 14 to 16-week-old 129 ReJ/++ and 129 ReJ/dydy mice. The grafted dystrophic muscle is able to produce and maintain for 100 days post-transplantation 356 +/- 22 myofibers, a number similar to that found in age-matched dystrophic muscle. The ability of old dystrophic muscle to regenerate subsequent to extreme trauma indicates that the cessation of "spontaneous" regeneration is due to factor(s) other than the exhaustion of mitotic capability of myosatellite cells. Moreover, there is no significant difference in myosatellite cell frequencies between grafted normal and dystrophic muscles (100 days post-transplantation). Myosatellite cell frequencies in grafted muscles are similar to those in age-matched, untraumatized muscles. While grafting of young dystrophic muscle modifies the phenotypic expression of histopathological changes usually associated with murine dystrophy, grafts of older dystrophic muscle show extensive connective-tissue infiltration and significantly fewer myofibers than do grafts of age-matched normal muscle. As early as 14 days post-transplantation, it is possible to distinguish between grafts of old, normal and dystrophic muscles. It is suggested that the connective tissue stroma, present in the dystrophic muscle at the time of transplantation, may survive the grafting procedure.
Bourke
D L
DL
Department of Neurobiology, Anatomy and Cell Science, University of Pittsburgh, School of Medicine, Pennsylvania 15261.
Ontell
M
M
Taylor
F
F
D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D051379:Mice; D008854:Microscopy, Electron; D008938:Mitosis; D009132:Muscles / Q000503:physiopathology* / Q000637:transplantation; D009137:Muscular Dystrophy, Animal / Q000503:physiopathology*; D009210:Myofibrils / Q000473:pathology; D012038:Regeneration; D014184:Transplantation, Homologous