Intestinal absorption of dietary fat from a liquid diet perfused in rats at a submaximum level.
The small intestine of rats was perfused in vivo for 2 h with a nutritionally complete liquid diet (68% calories from fat as corn oil). As the perfusion increased from 106 mg/2 h, the intestinal disappearance of the 14C-triolein marker remained proportional to the load up to 2,359 mg fat/2 h. Despite a decrease in absorption from 70 to 17%, this represents a very large fat intake. Fat absorption improved when medium-chain triglycerides or octanoic acid replaced corn oil (both p less than 0.01). Linoleic acid was absorbed from the diet less than corn oil (p less than 0.01). Dry ox bile reduced fat absorption (p less than 0.05); lipase and an antacid had no effect. Corn oil perfused alone was absorbed better than from the diet (p less than 0.01). Data with 14C-triolein was confirmed by dry-weight disappearance of the diet and by net intestinal water balance. Usual feeding underutilizes a large reserve for fat absorption. This reserve should be considered in therapeutic nutrition.
Simko
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Department of Medicine, University of Cincinnati, OH.
Kelley
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RE
D000818:Animals; D000863:Antacids / Q000494:pharmacology; D001646:Bile / Q000502:physiology; D002250:Carbon Radioisotopes; D004041:Dietary Fats / Q000008:administration & dosage* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects; D008049:Lipase / Q000494:pharmacology; D008297:Male; D010477:Perfusion / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions
D000863:Antacids; D002250:Carbon Radioisotopes; D004041:Dietary Fats; D012996:Solutions; D000431:Ethanol; D008049:Lipase