title: Detection of mononuclear cells expressing hepatitis B virus in peripheral blood from HBsAg positive and negative patients by in situ hybridisation.
abstract: In situ hybridisation was used to detect RNA of Hepatitis B virus (HBV) in peripheral mononuclear blood cells. Presence of HBV-RNA was detected in 10/17 HBsAg positive HBeAg positive patients, in 8/18 HBsAg positive HBeAg negative patients, in 1/3 anti-HBs positive subjects. Four control subjects were negative. The frequency of labelled cells varied from 1 to 10% of mononuclear cells. These results indicate that the DNA detected so far in mononuclear blood cells may be transcriptionally active.
authors.affiliation: Inserm U 56, Hôpital de Bicêtre, France.
authors.forename: M
authors.initials: M
authors.lastname: Hadchouel
authors.forename: C
authors.initials: C
authors.lastname: Pasquinelli
authors.forename: J G
authors.initials: JG
authors.lastname: Fournier
authors.forename: R N
authors.initials: RN
authors.lastname: Hugon
authors.forename: J
authors.initials: J
authors.lastname: Scotto
authors.forename: O
authors.initials: O
authors.lastname: Bernard
authors.forename: C
authors.initials: C
authors.lastname: Brechot
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004274:DNA, Recombinant; D004279:DNA, Viral; D006509:Hepatitis B / Q000382:microbiology*; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000032:analysis / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D012367:RNA, Viral