title: Mechanism of the decrease in hexose transport by mouse mammary epithelial cells caused by fasting.
abstract: The basal carrier-mediated uptake of 0.5 mM-3-O-methylglucose by mammary epithelial cells from lactating mice was calculated to be 227 +/- 9 pmol/min per microgram of DNA (mean +/- S.E.M., n = 11). Fasting the mice for 16 h overnight resulted in a decrease in this rate to 65 +/- 4 pmol/min per microgram of DNA (n = 10). Refeeding the fasted mouse for 3 h before isolation of the cells restored the transport activity to 230 +/- 12 pmol/min per microgram of DNA (n = 12). The Vmax. for equilibrium exchange entry of 3-O-methylglucose by intact cells was decreased from 6.6 +/- 0.4 to 0.9 +/- 0.2 nmol/min per microgram of DNA (mean +/- S.E.M., n = 3) by fasting. The number of D-glucose-inhibitable cytochalasin-B-binding sites in a plasma-membrane-enriched fraction of the cells was also decreased from 5.7 +/- 1.5 to 1.7 +/- 0.1 pmol/mg of membrane protein (mean +/- S.E.M., n = 3). Again, refeeding the fasted mouse for 3 h reversed both these effects. These results are consistent with a decrease in the number of functional glucose carriers in the plasma membrane of the mammary epithelial cells. Since the restoration of transporter activity after refeeding does not appear to require the synthesis of new protein, the effect of fasting probably involves not a loss of transporters, but a change in their orientation within the plasma membrane or a redistribution within the cell.
authors.affiliation: Laboratory of Biochemistry and Metabolism, National Institutes of Health, Bethesda, MD 20892.
authors.forename: C G
authors.initials: CG
authors.lastname: Prosser
mesh_terms: D019325:3-O-Methylglucose; D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D003571:Cytochalasin B / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005215:Fasting; D005260:Female; D005502:Food; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008757:Methylglucosides / Q000493:pharmacokinetics*; D008759:Methylglycosides / Q000493:pharmacokinetics*; D051379:Mice; D008809:Mice, Inbred C3H; D011247:Pregnancy; D011485:Protein Binding
chemical_list: D008565:Membrane Proteins; D008757:Methylglucosides; D008759:Methylglycosides; D019325:3-O-Methylglucose; D003571:Cytochalasin B