title: Further inhibition studies on guanidinobenzoatase, a trypsin-like enzyme associated with tumour cells.
abstract: Guanidinobenzoatase is a proteolytic enzyme capable of degrading fibronectin and is a tumour associated enzyme. Guanidinobenzoatase has been shown to be an arginine selective protease and is distinct from trypsin, plasminogen activator, plasmin, thrombin and a newly described tumour associated enzyme specific for guanidino phenylalanine residues. These conclusions have been derived from inhibition studies employing 4-methyl-p-guanidinobenzoate as substrate. Three active site titrants for trypsin have been shown to be good substrates for guanidinobenzoatase. A new active site titrant for trypsin, rhodamine bisguanidinobenzoate, can also be used to assay guanidinobenzoatase in a stoichiometric manner. This active site titrant can be employed to label guanidinobenzoate on the surface of leukaemia cells.
authors.affiliation: Department of Biochemistry, University of Manchester, UK.
authors.forename: F S
authors.initials: FS
authors.lastname: Steven
authors.forename: M M
authors.initials: MM
authors.lastname: Griffin
authors.forename: T L
authors.initials: TL
authors.lastname: Wong
authors.forename: R
authors.initials: R
authors.lastname: Yasmin
authors.forename: W F
authors.initials: WF
authors.lastname: Mangel
mesh_terms: D000818:Animals; D001665:Binding Sites; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D010450:Endopeptidases; D007700:Kinetics; D051379:Mice; D011480:Protease Inhibitors / Q000494:pharmacology*; D014357:Trypsin
chemical_list: D011480:Protease Inhibitors; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases; D014357:Trypsin