Characterization of neurophysin in the anterior pituitary gland of sheep.
Portions of sheep anterior pituitary lobe tissue were extracted under acid conditions and assayed for the two neurophysins (oN-III and oN-I/II) by radioimmunoassay. In all tissues examined, oN-III and oN-I/II immunoreactivities were detected. Using a combination of isoelectric focusing and polyacrylamide gel electrophoresis, the neurophysins of the anterior pituitary gland behaved like oN-III and oN-I/II. oN-III of the anterior pituitary was purified by affinity chromatography and high-performance liquid chromatography. This corticotroph oN possessed an amino acid analysis similar to that of oN-III and an N-terminal amino acid sequence, including residues 1-24, identical to that of authentic oN-III. These findings support the work of others who have identified neurohypophysial hormones in the anterior pituitary gland.
Watkins
W B
WB
Postgraduate School of Obstetrics and Gynecology, National Women's Hospital, Auckland, New Zealand.
Moore
L G
LG
Spiess
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D005260:Female; D009481:Neurophysins / Q000032:analysis*; D010121:Oxytocin / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D012756:Sheep
D009481:Neurophysins; D001127:Arginine Vasopressin; D010121:Oxytocin