A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae and Escherichia coli.
A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae M5a1 and Escherichia coli K12 has been developed. The method, which uses a freeze-thaw cycle in the presence of CaCl2 to facilitate DNA uptake, is substantially more efficient for K. pneumoniae M5a1 than the conventional transformation procedure for E. coli. The simplicity and speed of the method makes it very attractive for routine transformation of K. pneumoniae M5a1 and E. coli K12.
Merrick
M J
MJ
AFRC Unit of Nitrogen Fixation, University of Sussex, Brighton, UK.
Gibbins
J R
JR
Postgate
J R
JR
D004926:Escherichia coli / Q000235:genetics*; D005615:Freezing; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008722:Methods; D010957:Plasmids; D013696:Temperature; D014169:Transformation, Bacterial