Retinal wholemounts: a simple method for precise mapping.
A simple and inexpensive method to analyse retinal wholemounts is described. It allows the definition of regularly spaced sites to map regional specializations of the retina under light microscopy. After attaching a piece of translucent millimetre graph paper behind the slide holding the preparation, the two grid patterns of the graph paper and of the eye-piece grid are used in combination for the definition of sites for analysis and the return to specific areas as small as 50-100 microns2. The present method is simpler and offers several advantages when compared to others based on X-Y movements of the microscope stage or on photographic montage.
Giorgi
P P
PP
Neuroembryology Laboratory, School of Anatomy, University of Queensland, Brisbane, Australia.
Graydon
M L
ML
D005069:Evaluation Studies as Topic; D006652:Histological Techniques / Q000592:standards; D012160:Retina / Q000033:anatomy & histology* / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000166:cytology