A rapid and simple method for electron microscopy of paraffin-embedded tissue.
A new rapid and simple method for electron microscopy of paraffin-embedded material is described. The paraffin-embedded tissue is deparaffinized in xylene, stained in a 0.01% toluidine blue/absolute ethanol solution, infiltrated in a propylene oxide/resin mixture, and embedded in epoxy resin. This quick method, which excludes rehydration, postosmification, and dehydration, takes about 3 h and produces results equal to previously described more laborious re-embedding techniques.
Widéhn
S
S
Department of Pathology, Sahlgren Hospital, University of Göteborg, Sweden.
Kindblom
L G
LG
D006801:Humans; D007890:Leiomyosarcoma / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D010232:Paraffin; D012208:Rhabdomyosarcoma / Q000648:ultrastructure; D012983:Soft Tissue Neoplasms / Q000648:ultrastructure; D013048:Specimen Handling / Q000379:methods*
D010232:Paraffin