title: Human growth hormone in urine: development of an ultrasensitive radiometric assay.
abstract: An immunoradiometric assay for human growth hormone (HGH) has been developed which has a detection limit of 1 ng/l and can measure HGH in unextracted urine from normal children and adults. The assay is based on a two-step procedure, using a solid-phase goat-anti-HGH immunosorbent for immunoextraction and [125I]-labeled monoclonal HGH-antibody for detection and quantification. The assay is not affected by urea, NaCl or changes of pH from 5-8. The mean urine HGH concentration in normal children is 6.78 +/- 7.6 (SD) pg/ml, in patients with HGH-deficiency 1.3 +/- 0.9 pg/ml which increases to 11.7 +/- 13.4 pg/ml on the day of growth hormone injection.
authors.affiliation: University Children's Hospital, Basel, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Erb
authors.forename: G
authors.initials: G
authors.lastname: Karolyi
authors.forename: A
authors.initials: A
authors.lastname: Pampalone
authors.forename: A N
authors.initials: AN
authors.lastname: Eberle
authors.forename: J B
authors.initials: JB
authors.lastname: Baumann
authors.forename: E
authors.initials: E
authors.lastname: Bürgisser
authors.forename: J
authors.initials: J
authors.lastname: Girard
mesh_terms: D005069:Evaluation Studies as Topic; D013006:Growth Hormone / Q000652:urine*; D006801:Humans; D009994:Osmolar Concentration; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards
chemical_list: D013006:Growth Hormone