title: Properties of component X of rat heart pyruvate dehydrogenase complex.
abstract: Pyruvate dehydrogenase complex was purified from rat heart. A new component(mol.wt; 52,000) was found in the purified complex in addition to well known three component enzymes. This component(referred to as component X) was acetylated with [2-14C] pyruvate in the absence of CoA as well as lipoate acetyltransferase. The anti-lipoate acetyltransferase antibody reacted with component X and lipoate acetyltransferase, suggesting that component X shows homology with lipoate acetyltransferase in protein structure. cDNA for lipoate acetyltransferase was isolated from rat liver cDNA library in lambda gt 11. cDNA for lipoate acetyltransferase recognized two kinds of mRNAs of 3.5 Kb and 2.5 Kb.
authors.affiliation: Department of Biology, Kanoya National Institute of Fitness and Sports, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matuda
authors.forename: K
authors.initials: K
authors.lastname: Nakano
authors.forename: I
authors.initials: I
authors.lastname: Tabata
authors.forename: S
authors.initials: S
authors.lastname: Matuo
authors.forename: T
authors.initials: T
authors.lastname: Saheki
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001482:Base Composition; D003001:Cloning, Molecular; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology*; D014176:Protein Biosynthesis; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051381:Rats
chemical_list: D000906:Antibodies; D011768:Pyruvate Dehydrogenase Complex; D000123:Acetyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase