Glycine potentiates N-methyl-D-aspartate-induced [3H]TCP binding to rat cortical membranes.
In extensively washed preparations of rat cortical membranes, N-methyl-D-aspartate (NMDA) increases the specific binding of [3H]TCP by over 4-fold in a concentration dependent manner (EC50 = 3.1 microM). Glycine (1 microM) potentiates the maximal effect of NMDA by a factor of 1.7. The effect of glycine is concentration dependent (EC50 = 380 nM) and strychnine insensitive. These data are discussed with reference to the recently reported effects of glycine on the NMDA operated cation channel and the relationship between the PCP and NMDA receptors.
Snell
L D
LD
University of Texas Medical Branch, Department of Pharmacology and Toxicology, Galveston 77550.
Morter
R S
RS
Johnson
K M
KM
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D004357:Drug Synergism; D004558:Electric Stimulation; D005998:Glycine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D016202:N-Methylaspartate; D010622:Phencyclidine / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D001224:Aspartic Acid; D016202:N-Methylaspartate; C012058:tenocyclidine; D010622:Phencyclidine; D005998:Glycine