title: Genotoxicity of compounds from cooked beef in repair-deficient CHO cells versus Salmonella mutagenicity.
abstract: A series of compounds isolated on the basis of their mutagenicity in the Ames/Salmonella reversion assay were previously identified in fried beef and chemically synthesized for further evaluation. In this study three of these compounds were tested for genotoxic effects in the UV5 line of Chinese hamster ovary (CHO) cells, which is deficient in nucleotide excision repair. Both 2-amino-3,4-dimethyl-imidazo]4,5-f]quinoline (MeIQ) and 2-amino-3,8-dimethyl-imidazo[4,5-f]quinoxaline (MeIQx) gave very weak responses for cell killing, hprt mutation induction and sister chromatid exchange. These effects occurred at doses in the range of 100-800 micrograms/ml (approximately solubility limit), and dose-dependent increases were not observed. Induction of chromosomal aberrations did not occur with either compound. Nor did either of these compounds produce differential cytotoxicity in normal CHO cells versus UV5 cells, indicating that potentially repairable DNA damage was not responsible for the observed cell killing. In contrast to these results, 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP), which constitutes greater than 90% of the mass of bacterial mutagens in beef, was strongly positive for all endpoints at doses in the range 1-3 micrograms/ml. PhIP also gave marked differential cytotoxicity (ratio of 6) and cell survival curves that were strongly dependent on repair capacity. Because PhIP is 50- to 300-fold less mutagenic than MeIQ and MeIQx in Salmonella TA1538, these results point to major differences between the bacterial and mammalian assays in terms of the relative potency of these food-related compounds.
authors.affiliation: Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA 94550.
authors.forename: L H
authors.initials: LH
authors.lastname: Thompson
authors.forename: J D
authors.initials: JD
authors.lastname: Tucker
authors.forename: S A
authors.initials: SA
authors.lastname: Stewart
authors.forename: M L
authors.initials: ML
authors.lastname: Christensen
authors.forename: E P
authors.initials: EP
authors.lastname: Salazar
authors.forename: A V
authors.initials: AV
authors.lastname: Carrano
authors.forename: J S
authors.initials: JS
authors.lastname: Felton
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D005260:Female; D006358:Hot Temperature; D008460:Meat; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology* / Q000633:toxicity; D009154:Mutation; D010053:Ovary; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D009153:Mutagens