The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth.
The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.
Kupiec
M
M
Department of Genetics, Hebrew University, Jerusalem, Israel.
D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004260:DNA Repair; D005800:Genes, Fungal; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development