title: Increased DNA replication in the arterial adventitia after aortic ligation.
abstract: After microsurgical dissection of the thoracic aorta of normotensive rats, biochemical and morphological comparisons were performed between the intima-media and adventitia. The DNA content, wet weight, and dry defatted weight of the adventitia were half that of the intima-media. Collagen was the main component of the adventitia (collagen greater than nonfibrous protein greater than elastin) whereas elastin was the main protein in the intima-media (elastin greater than nonfibrous protein greater than collagen), and the results correlated with morphological observations. Hypertension induced by aortic ligation between the renal arteries resulted in rapid elevations in circulating humoral factors and blood pressure. A sixfold increase in DNA synthesis was observed in the adventitia (p less than 0.001), resulting in a significant increase in DNA content as early as 6 days after aortic ligation (75% increase; p less than 0.001). Increased DNA replication was accompanied by elevations in nonfibrous protein and elastin contents. Autoradiograms showed labeled adventitial fibroblasts throughout the thickness of the adventitia and along the entire length of the aorta and smaller vessels. DNA synthesis and content and labeled smooth muscle cells were increased in the intima-media. These studies indicate that the adventitia participates in the development of vascular hypertrophy and arterial disease produced by aortic ligation.
authors.affiliation: Research Department, CIBA-GEIGY Corporation, Summit, New Jersey 07901.
authors.forename: R E
authors.initials: RE
authors.lastname: Chatelain
authors.forename: B N
authors.initials: BN
authors.lastname: Dardik
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D001794:Blood Pressure; D002455:Cell Division; D003094:Collagen / Q000032:analysis; D004247:DNA / Q000032:analysis; D004261:DNA Replication; D004549:Elastin / Q000032:analysis; D006973:Hypertension / Q000209:etiology* / Q000378:metabolism; D008026:Ligation; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003094:Collagen; D004247:DNA; D004549:Elastin