19F-nuclear magnetic resonance: measurements of [O2] and pH in biological systems.
19F-nuclear magnetic resonance (NMR) has been used to determine both intracellular pH and oxygen concentrations in cell suspensions. Oxygen concentrations in Paracoccus denitrificans and insulinoma cells, RINm5F, in the NMR probe can be monitored directly by 1/T1 measurements of perfluorotripropylamine (FTPA)/lecithin emulsion added to the suspensions. With FTPA oxygen monitoring, we investigated the relative aeration capabilities of two types of NMR chambers. Both normal and transformed eucaryotic cells can be maintained in either chamber for at least 1-2 h at cytocrits of up to 20-25%, with 30% oxygen saturation and cell viabilities of 90-95%. Similar concentrations of procaryotes were maintained aerobic with high FTPA concentrations in the more efficient of the two NMR chambers. A new precursor molecule for the 19F-NMR pH indicator difluoromethylalanine, the para-chlorophenyl ester, has been tested and used in RINm5F cells and P. denitrificans, neither of which hydrolyzes methyl esters.
Taylor
J
J
Department of Physiology, Univesity of Pennsylvania School of Medicine, Philadelphia 19104-6085.
Deutsch
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D000818:Animals; D002460:Cell Line; D005461:Fluorine; D005466:Fluorocarbons; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007340:Insulinoma; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption; D010190:Pancreatic Neoplasms; D010231:Paracoccus denitrificans / Q000032:analysis*; D051381:Rats
D005466:Fluorocarbons; D007202:Indicators and Reagents; D005461:Fluorine; C039422:perfluorotripropylamine; D010100:Oxygen