Isolation of an ompC-like outer membrane protein gene from Salmonella typhi.
We have isolated the structural gene for an outer membrane protein of Salmonella typhi, from a genomic library constructed in bacteriophage lambda 1059, using the Escherichia coli ompC gene as a heterologous probe. E. coli ompC codes for an outer membrane pore protein (porin) that is induced preferentially at high osmolarity and high temperature. The S. typhi ompC-like gene was subcloned in pBR322 and introduced into E. coli HB101 and into P678-54, a minicell-producing strain. In both strains it expressed a 38.5-kDa protein, which was incorporated into the outer membrane envelope and comigrated with an S. typhi outer membrane protein which was expressed both at low and high osmolarity in vivo.
Puente
J L
JL
Departamento de Biología Molecular, Universidad Nacional Autónoma de México.
Flores
V
V
Fernández
M
M
Fuchs
Y
Y
Calva
E
E
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D012485:Salmonella typhi / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins