Detection of N-acetyl derivative of 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) in Glu-P-1-injected rats.
In order to confirm in vivo N-acetylation of 2-amino-6-methyl-dipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) in rats, we developed a new high-performance liquid chromatography (HPLC) method for detecting the N-acetyl derivative of Glu-P-1 in animal organs. Using this method, N-acetyl-Glu-P-1 was detected in rat liver, kidney and intestinal contents 6 h after intraperitoneal injection of Glu-P-1. This fact provides evidence to support in vivo N-acetylation of Glu-P-1 in rats.
Kanai
Y
Y
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Manabe
S
S
Wada
O
O
D000107:Acetylation; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007093:Imidazoles / Q000032:analysis* / Q000633:toxicity*; D008297:Male; D013058:Mass Spectrometry; D009153:Mutagens / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D007093:Imidazoles; D009153:Mutagens; C023785:2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole