Evaluation of a single-color-reading method for determining fructosamine.
A commercial kit for determining fructosamine was evaluated. The reference interval (determined from data on 183 nondiabetic subjects) was 1.67 to 2.85 (mean 2.17) mmol/L. Serum and plasma (EDTA- or heparin-anticoagulated) gave equivalent results; plasma treated with fluoride/oxalate gave slightly lower values. The between-run CV was less than 4%. Fructosamine values were similar by the present method and the kinetic method. The standard curve was linear in the range of 1.3 to 8.5 mmol/L. None of several constituents of blood that we tested appreciably interfered. Fructosamine values were increased in some lipemic samples from non-diabetics, and were significantly correlated with glycated hemoglobin as measured by affinity chromatography. This kit evidently is a suitable alternative to the kinetic method for determining fructosamine.
Rosenthal
M A
MA
Isolab, Inc., Akron, OH 44321.
Olenick
L J
LJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001322:Autoanalysis; D001663:Bilirubin / Q000097:blood; D005260:Female; D019270:Fructosamine; D006442:Glycated Hemoglobin / Q000378:metabolism; D006461:Hemolysis; D006595:Hexosamines / Q000097:blood*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000032:analysis; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D013053:Spectrophotometry
D001663:Bilirubin; D019270:Fructosamine; D006442:Glycated Hemoglobin; D006595:Hexosamines; D011933:Reagent Kits, Diagnostic