Stable isotope dilution assay for prostaglandin E metabolite: 24-hour urinary output in healthy male subjects.
A stable isotope dilution assay for the major urinary metabolite of prostaglandins E1 and E2 (PGE-M) was developed. After extraction and purification, the compound was converted to the dimethyl ester bis(O-methyloxime)-trimethylsilyl ether derivative and quantified with the 13C-labeled dimethyl ester analog as internal standard. The interassay coefficient of variation was 0.8%, and the intraassay coefficient of variation ranged from 0.9 to 1.7%. The recovery of authentic, underivatized PGE-M added to urine was 99.5 +/- 3.2% (mean +/- SE, N = 9). The equation of the regression curve correlating the amounts added and recovered was Y = 1.05X - 5.84, with R = 0.998. The level of excreted metabolite in healthy male subjects was 13.86 +/- 1.46 micrograms/24 h (N = 24), in good agreement with data from other sources.
Ferretti
A
A
United States Department of Agriculture, Beltsville Human Nutrition Research Center, Lipid Nutrition Laboratory, Maryland 20705.
Flanagan
V P
VP
Reeves
V B
VB
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007201:Indicator Dilution Techniques; D008297:Male; D008875:Middle Aged; D011458:Prostaglandins E / Q000378:metabolism / Q000652:urine*
D011458:Prostaglandins E