title: Effect of some thiol reagents on erythrocyte adenosine deaminase (ADA) activity.
abstract: The effect of three thiol reagents on erythrocyte adenosine deaminase (ADA) activity has been studied. Oxidized glutathione and iodoacetate do not alter ADA activity, while the treatment with p-chloromercuribenzoate at similar concentrations results in a reduction of enzymatic activity which is statistically significant only for ADA 1, but not ADA 2-1 phenotype haemolysates.
authors.affiliation: Department of Genetics and Molecular Biology, La Sapienza University, Rome, Italy.
authors.forename: R M
authors.initials: RM
authors.lastname: Corbo
authors.forename: R
authors.initials: R
authors.lastname: Scacchi
authors.forename: E
authors.initials: E
authors.lastname: Mantuano
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood*; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D007527:Isoenzymes / Q000097:blood*; D007700:Kinetics; D009700:Nucleoside Deaminases / Q000097:blood*; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D020245:p-Chloromercuribenzoic Acid
chemical_list: D002729:Chloromercuribenzoates; D007461:Iodoacetates; D007527:Isoenzymes; D013439:Sulfhydryl Reagents; D020245:p-Chloromercuribenzoic Acid; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D005978:Glutathione; D019807:Iodoacetic Acid