title: Interaction of parvalbumin of pike II with calcium and terbium ions.
abstract: Fluorimetric titrations of parvalbumin II (pI 4.2) of pike (Pike II) with Ca2+ and Tb3+ show the CD and EF binding sites to be non-equivalent. The intrinsic binding constants of the strong and the weak sites obtained for Ca2+ are: KsCa = 1.6.10(8) M-1; KwCa = 6.6.10(5) M-1. Differences of the order of 100% were encountered between the Tb3+ binding constants obtained with four different versions of titration. Their average values are: KsTb = 1.9.10(11) M-1; KwTb = 1.0.10(7) M-1. The distances of the strong and the weak sites from the singular Tyr-48, rs = 9.5 A and r2 = 11.5 A, were derived from Förster-type energy transfer and proved compatible with the X-ray structure of parvalbumin III (pI 4.2) of carp (CarpIII). From the distances, it is suggested that CD is the strong and EF the weak metal-binding site of PikeII. Tb3+ was shown by CD spectroscopy to have the same structural effect on PikeII as Ca2+. Removal of the metal ions from PikeII results in a decrease of helix content as monitored by CD spectroscopy. This decrease is larger than that in CarpIII. A concomitant decrease of the fluorescence quantum yield at nearly constant decay time is indicative of mainly static quenching, probably by the non-coordinating carboxylate groups. The maximum helix content is almost completely reestablished upon binding of the first metal ion. However, small changes of the energy transfer in PikeII with one terbium ion bound to the strong site indicate fine structural rearrangements of the strong binding site when Ca2+ is bound to the weak one.
authors.affiliation: Abteilung Physiologische Chemie, Rheinisch-Westfälische Technische Hochschule Aachen, F.R.G.
authors.forename: I
authors.initials: I
authors.lastname: Eberspach
authors.forename: W
authors.initials: W
authors.lastname: Strassburger
authors.forename: U
authors.initials: U
authors.lastname: Glatter
authors.forename: C
authors.initials: C
authors.lastname: Gerday
authors.forename: A
authors.initials: A
authors.lastname: Wollmer
mesh_terms: D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D009124:Muscle Proteins / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012487:Salmonidae / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000378:metabolism*
chemical_list: D008024:Ligands; D009124:Muscle Proteins; D010320:Parvalbumins; D013725:Terbium; D002118:Calcium