Complement activation by eggs and microfilariae of filarial parasites.
The complement of fresh normal rat serum was activated by filarial eggs and microfilariae (mf). C3 was deposited on the surface of Litomosoides carinii, Brugia pahangi, Brugia malayi and Dipetalonema viteae as seen by immunofluorescence. Intra-uterine and in vitro-derived mf did not bind C3. In contrast, C3 bound to the blood-derived mf of B. pahangi and B. malayi as well as exsheathed mf of L. carinii and B. malayi. Significant consumption of complement was observed with eggs of all filarial species, as well as sheathed mf of B. pahangi, B. malayi and exsheathed mf of L. carinii and B. malayi. These experiments indicated that complement was activated by filarial parasites via the alternative pathway. The bound complement promoted neutrophil-mediated adherence and cytotoxicity.
Rao
U R
UR
Research Centre, Pharma Division, Hindustan CIBA-Geigy Limited, Goregaon, Bombay, India.
Chandrashekar
R
R
Subrahmanyam
D
D
D000818:Animals; D002009:Brugia / Q000276:immunology; D002448:Cell Adhesion; D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism; D003170:Complement Pathway, Alternative; D003602:Cytotoxicity, Immunologic; D004153:Dipetalonema / Q000276:immunology; D005370:Filarioidea / Q000276:immunology*; D066298:In Vitro Techniques; D016177:Macrophage-1 Antigen; D008842:Microfilariae / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010063:Ovum / Q000276:immunology; D051381:Rats; D011951:Receptors, Complement / Q000276:immunology
D003176:Complement C3; D016177:Macrophage-1 Antigen; D011951:Receptors, Complement