The Saccharomyces cerevisiae MET3 gene: nucleotide sequence and relationship of the 5' non-coding region to that of MET25.
In Saccharomyces cerevisiae, the expression of several genes implicated in methionine biosynthesis is co-regulated by a specific negative control. To elucidate the molecular basis of this regulation, we have cloned two of these genes, MET3 and MET25. The sequence of MET25 has already been determined (Kerjan et al. 1986). Here, we report the nucleotide sequence of the MET3 gene along with its 5' and 3' flanking regions. Plasmids bearing different deletions upstream of the transcribed region of MET3 were constructed. They were introduced into yeast cells and tested for their ability to complement met3 mutations and to respond to regulation by exogenous methionine. The regulatory region was located within a 100 bp region. The sequence of this regulatory region was compared with that of MET25. A short common sequence which occurs 250-280 bp upstream of the translation initiation codon of the gene was found. This sequence is a good candidate for the cis-acting regulatory element.
Cherest
H
H
Laboratoire d'Enzymologie du C.N.R.S., Gif-sur-Yvette, France.
Kerjan
P
P
Surdin-Kerjan
Y
Y
D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D003062:Codon / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D005809:Genes, Regulator; D008715:Methionine / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013430:Sulfate Adenylyltransferase / Q000235:genetics
D003062:Codon; D004271:DNA, Fungal; D008715:Methionine; D013430:Sulfate Adenylyltransferase