title: Lysosomal alterations during coronary atherosclerosis in the pigeon: correlative cytochemical and three-dimensional HVEM/IVEM observations.
abstract: Lysosomal changes have been implicated as one of the major factors contributing to the progression and complications of atherosclerosis, and recently foam cell formation has been correlated with increases in several acid hydrolases. To explore at the subcellular level relationships among lesion progression, cellular lipid accumulation, and lysosomal change, atherosclerotic lesions from hypercholesterolemic White Carneau pigeons have been studied through combined ultrastructural cytochemistry and stereo (three-dimensional) high-voltage electron microscopy. Lysosomal enzyme activity in the prelesion intima and in foam cells of early lesions was in discrete lysosomes of macrophage foam cells. Foam cell lipid at the early stages was primarily (72%) in cytoplasmic droplets, which formed a three-dimensional network with the small (0.25-0.8 microM in diameter), reaction-positive lysosomes suspended at the vertices of a cytoplasmic lattice that delineated individual lipid pools. Concomitant with lesion progression and increasing complexity, foam cell lysosome number, size, and complexity increased. The complexity was characterized by lysosome lipid accumulation (60% of cell lipid) and the fusion of lysosomes to form multilobulated organelles in which the acid phosphatase reaction product typically was circumferential to the lysosomal lipid core. The involvement of lysosomes climaxed in the more advanced region of lesions with foam cells in which the bulk of cytoplasmic volume was occupied by large (15-20 microM in diameter), multicompartmental, lipid-containing lysosomes. It is suggested that this progressive involvement of lysosomes is responsible for cell and tissue necroses characteristic of advanced lesions.
authors.affiliation: Department of Pathology, Wake Forest University, Bowman Gray School of Medicine, Winston-Salem, NC 27103.
authors.forename: J C
authors.initials: JC
authors.lastname: Lewis
authors.forename: R G
authors.initials: RG
authors.lastname: Taylor
authors.forename: K
authors.initials: K
authors.lastname: Ohta
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001161:Arteriosclerosis / Q000473:pathology*; D010856:Columbidae; D003327:Coronary Disease / Q000473:pathology*; D003331:Coronary Vessels / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure*; D004036:Diet, Atherogenic; D008055:Lipids / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods
chemical_list: D008055:Lipids; D000135:Acid Phosphatase