Detection of short-chain carbonyl products of lipid peroxidation from malaria-parasite (Plasmodium vinckei)-infected red blood cells exposed to oxidative stress.
Reversed-phase h.p.l.c. was used to detect 2,4-dinitrophenylhydrazine-reactive carbonyl products, which excludes malonaldehyde, in malaria-parasite (Plasmodium vinckei)-infected murine red blood cells (RBCs). A number of alkanals, 4-hydroxyalk-2-enals and alka-2,4-dienals were tentatively identified by comparison with authentic standards. The formation of 4-hydroxynon-2-enal, deca-2,4-dienal and hexanal was greater in P. vinckei-infected RBCs than in their uninfected counterparts and was increased by the presence of t-butyl hydroperoxide. Several of these aldehydes have previously been shown to be toxic to various types of cells, including P. falciparum, in vitro. The iron chelator desferrioxamine and the free-radical scavenger butylated hydroxyanisole inhibited the formation of these aldehydes. These experiments demonstrate that products of lipid peroxidation other than malonaldehyde are formed during the exposure of malaria-infected RBCs in vitro to drugs that generate reactive oxygen species and have anti-parasitic activity. The formation of products of this type during the natural course of malaria infection may have implications for the mechanisms underlying intra-RBC parasite death and the tissue damage associated with the disease.
Buffinton
G D
GD
Department of Experimental Pathology, John Curtin School of Medical Research, Canberra, Australia.
Hunt
N H
NH
Cowden
W B
WB
Clark
I A
IA
D000447:Aldehydes / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism* / Q000469:parasitology; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000097:blood*; D008288:Malaria / Q000097:blood* / Q000469:parasitology; D051379:Mice; D008808:Mice, Inbred CBA; D010084:Oxidation-Reduction; D010545:Peroxides / Q000494:pharmacology; D010659:Phenylhydrazines; D020122:tert-Butylhydroperoxide
D000447:Aldehydes; D008054:Lipid Peroxides; D010545:Peroxides; D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine; D020122:tert-Butylhydroperoxide