Clones from two different genomic regions complement the cdc25 start mutation of Saccharomyces cerevisiae.
We have cloned the CDC25 gene of Saccharomyces cerevisiae whose product is required for traversing the Go phase of the cell cycle. A preliminary physical characterization of the CDC25 gene region is presented. In addition, we show that another gene, when cloned in a high-copy number plasmid, is able to partially suppress growth thermosensitivity of a strain carrying the cdc25 mutation. We briefly discuss the possible interaction of these gene products with adenylate cyclase encoded by the CDC35 gene.
Daniel
J
J
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Simchen
G
G
D000483:Alleles; D002453:Cell Cycle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005800:Genes, Fungal; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D013489:Suppression, Genetic