[Antibiotics and chemotaxis in agarose. The role of incubation conditions and application to several fluoroquinolones].
Nelson's agarose technique for the in vitro measurement of leukocyte chemotaxis proved to be well suited in the context of pharmaco-toxicological testings, despite several discrepancies in the literature, probably due to varied incubation conditions. Thus, doxycycline (25 micrograms/ml) when added to both the leukocyte suspension and the agarose was shown to exert a significantly more pronounced depressant effect. Using these conditions of improved sensitivity, three fluoroquinolones, i.e. norfloxacin, ofloxacin and pefloxacin (2, 10 or 25 micrograms/ml) failed to depress chemotaxis markedly.
Descotes
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Unité mixte INSERM U80/CNRS UA 1177/UCBL, Laboratoire de Pharmacologie, Faculté de Médecine Alexis Carrel, Lyon.
Lombard
J Y
JY
D000890:Anti-Infective Agents / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004318:Doxycycline / Q000494:pharmacology*; D006801:Humans; D009504:Neutrophils / Q000276:immunology; D009643:Norfloxacin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D015242:Ofloxacin; D010078:Oxazines / Q000494:pharmacology*; D015366:Pefloxacin; D012685:Sepharose
D000890:Anti-Infective Agents; D010078:Oxazines; D015366:Pefloxacin; D012685:Sepharose; D015242:Ofloxacin; D009643:Norfloxacin; D004318:Doxycycline
Antibiotiques et chimiotactisme en agarose. Rôle des conditions d'incubation et application à plusieurs fluoroquinolones.