title: Promoters of Mycoplasma capricolum ribosomal RNA operons: identical activities but different regulation in homologous and heterologous cells.
abstract: The 5' region of the rRNA operon, rrnA, of M. capricolum was cloned. Sequence analysis revealed two tRNA genes, tRNA(leu) and tRNA(lys), upstream to the promoter of the rRNA operon. The in vivo transcription start sites of the rRNA operon and of the tRNA genes were mapped. The same promoters used by M. capricolum RNA polymerase are also recognized by E. coli RNA polymerase both in vivo and in vitro. We find that high levels of ppGpp in E. coli, resulting from amino acid starvation or from spoT mutation, activate rather than repress the transcription of the mycoplasma rrnA operon.
authors.affiliation: Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Gafny
authors.forename: H C
authors.initials: HC
authors.lastname: Hyman
authors.forename: S
authors.initials: S
authors.lastname: Razin
authors.forename: G
authors.initials: G
authors.lastname: Glaser
mesh_terms: D001483:Base Sequence; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D009876:Operon; D012335:RNA, Ribosomal / Q000235:genetics*; D012356:RNA, Transfer, Leu / Q000235:genetics; D012357:RNA, Transfer, Lys / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012335:RNA, Ribosomal; D012356:RNA, Transfer, Leu; D012357:RNA, Transfer, Lys