Immunoscintigraphic localization of inflammatory lesions: concept, radiolabelling and in vitro testing of a granulocyte specific antibody.
Current nuclear medicine techniques for the localization of inflammatory processes are based on injection of 111In labelled autologous granulocytes which need to be isolated and radiolabelled in vitro before reinjection. A new technique is presented here that obviates the need for cell isolation by the direct intravenous injection of a granulocyte specific 123I labelled monoclonal antibody. In this publication the basic parameters of the antibody granulocyte interaction are described. Antibody binding does not inhibit vital functions of the granulocytes, such as chemotaxis and superoxide generation. Scatchard analysis of binding data reveals an apparent affinity of the antibody for granulocytes of 6.8 X 10(9) l/mol and approximately 7.1 X 10(4) binding sites per cell. Due to the high specificity of the antibody, the only expected interference is from CEA producing tumors.
Andres
R Y
RY
Swiss Federal Institute for Reactor Research, Würenlingen.
Schubiger
P A
PA
Tiefenauer
L
L
Seybold
K
K
Locher
J T
JT
Mach
J P
JP
Buchegger
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000915:Antibody Affinity; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D005260:Female; D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000000981:diagnostic imaging*; D007457:Iodine Radioisotopes; D008722:Methods; D011877:Radionuclide Imaging; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007457:Iodine Radioisotopes