Anti-Ro (SS-A) antibodies from Ro (SS-A)-immunized mice.
Immunization of BALB/c mice with immunoaffinity-purified bovine or human Ro (SS-A) induces the production of antibodies reactive with Ro (SS-A). Fusion of spleen cells from the hyperimmunized mice to SP2/0 cells resulted in hybridoma cell lines that produced anti-Ro (SS-A) antibodies. Anti-Ro (SS-A) binding was established by solid-phase immunosorbent assay, immunoblotting, or RNA immunoprecipitation. Most of the anti-Ro (SS-A) antibodies bound to both human and bovine Ro (SS-A) in the solid phase, but only one of the monoclonal antibodies selectively bound to human Ro (SS-A); this suggests that there are species differences between the bovine and human Ro (SS-A) antigens. Indirect immunofluorescence studies demonstrated that most anti-Ro (SS-A) antibodies bound to cytoplasmic or nuclear HEp-2 cellular antigens, whereas others did not bind to fixed HEp-2 tissue culture cells. Nuclear staining of mouse substrates by one of the sera containing anti-Ro (SS-A) demonstrated that autoantibodies were induced by immunization with human Ro (SS-A).
Rosario
M O
MO
Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City.
Fox
O F
OF
Koren
E
E
Harley
J B
JB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000917:Antibody Formation; D001323:Autoantibodies / Q000276:immunology* / Q000378:metabolism; D001324:Autoantigens / Q000276:immunology*; D001666:Binding Sites, Antibody; D005260:Female; D006825:Hybridomas; D007114:Immunization; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human