Pharmacology and molecular identification of secretin receptors in rat gastric glands.
The structure of the secretin receptor in purified plasma membranes isolated from the antral and fundic parts of the rat gastric mucosa was probed, using the cross linking reagent dithiobis succinimidyl propionate (DSP) and HPLC-purified [125I] secretin. [125I] secretin binding sites were preferentially located in rat antrum and displayed the pharmacological properties expected for specific secretin receptors: secretin greater than helodermin greater than rhGRF greater than rPHI. SDS gel electrophoresis of the solubilized receptor allowed identification of two radiolabeled peptides of 62 and 33 KDa connected by disulfide bonds. According to the sensitivity of the 62 KDa component to low doses of secretin and to GTP, it constitutes the membrane domain involved in the physiological regulation of adenylate cyclase by secretin in rat gastric glands.
Bawab
W
W
INSERM U. 55, Unité de Recherche sur les Peptides Neurodigestifs et le Diabète, Hôpital Saint-Antoine, Paris, France.
Gespach
C
C
Marie
J C
JC
Chastre
E
E
Rosselin
G
G
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D005753:Gastric Mucosa / Q000032:analysis* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D043562:Receptors, G-Protein-Coupled; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis* / Q000378:metabolism; D012633:Secretin / Q000378:metabolism*; D013388:Succinimides / Q000494:pharmacology
D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D043562:Receptors, G-Protein-Coupled; D011964:Receptors, Gastrointestinal Hormone; D013388:Succinimides; C029937:secretin receptor; D012633:Secretin; D006160:Guanosine Triphosphate; C011240:dithiobis(succinimidylpropionate)