title: Interaction of plasma proteins with negatively charged sites on the pulmonary capillary endothelium of the rat.
abstract: The endothelial glycocalyx, a polyanionic structure which may regulate the passage of solutes and water through the endothelium, readily binds cationic ferritin (CF). In normal, nonexchange-transfused rats, however, only 7.5% and 6.0% of the luminal plasma membrane and 7.5% and 5.0% of vesicle diaphragms on the thick and thin side of pulmonary capillaries, respectively, bound cationic ferritin. With the graded removal of circulating proteins by exchange transfusion with fluorocarbon emulsion, up to 89 and 82% of the luminal surface, and 76 and 73% of vesicle diaphragms on the thick and thin sides, respectively, bound CF. Although the extent of binding on the thin side was consistently less than on the thick side, the difference was not statistically significant. The extensive binding of CF to the glycocalyx in totally exchange-transfused rats was completely reversible upon addition of lyophilized rat serum protein to the perfusate. These data suggest that in vivo anionic sites of the endothelial glycocalyx are partially masked by adsorbed plasma proteins.
authors.affiliation: Department of Pathology, Massachusetts General Hospital, Boston 02114.
authors.forename: E E
authors.initials: EE
authors.lastname: Schneeberger
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002196:Capillaries / Q000378:metabolism / Q000648:ultrastructure; D004727:Endothelium / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D005293:Ferritins / Q000378:metabolism; D005466:Fluorocarbons; D006023:Glycoproteins / Q000378:metabolism*; D008168:Lung / Q000098:blood supply*; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000838:Anions; D001798:Blood Proteins; D005466:Fluorocarbons; D006023:Glycoproteins; D011134:Polysaccharides; C010297:polycationic ferritin; D005293:Ferritins