H1-histaminergic activation of catecholamine release by chromaffin cells.
Bovine adrenal medullary chromaffin cells, prelabeled with [3H]norepinephrine, released a large proportion of cellular 3H-labeled catecholamines (CAs) when stimulated with nicotine, K+, histamine, gamma-aminobutyric acid (GABA) and several peptidic hormones [bradykinin, angiotensin II, thyrotropin releasing hormone (TRH) and neurotensin]. The histamine-induced response was dose dependent and occurred through H1 histaminergic receptors. Quantitatively and temporally the histamine- and nicotine-induced responses differed. Nicotine, during the first minutes, induced a large increase of [3H]CAs, but this response was desensitized rapidly. In contrast, histamine initially provoked a smaller release of [3H]CAs than nicotine but, with prolonged exposure (hours), a much greater response was found with histamine. Moreover, little desensitization was observed with histamine even during extended stimulation. External Ca2+ was obligatory for the histamine response, and both inorganic (Co2+ and Ni2+) and organic (verapamil, nifedipine and D-600) Ca2+ channel blockers significantly reduced release of [3H]CAs. These studies suggest that histamine as well as certain other neuroactive substances could play an important role in the physiology and biochemistry of adrenal medullary chromaffin cells.
Noble
E P
EP
Department of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles 90024.
Bommer
M
M
Liebisch
D
D
Herz
A
A
D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D002927:Cimetidine / Q000494:pharmacology; D002974:Clemastine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D007700:Kinetics; D009116:Muscarine / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism; D011899:Ranitidine / Q000494:pharmacology; D011968:Receptors, Histamine / Q000502:physiology*; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000502:physiology*
D002395:Catecholamines; D006728:Hormones; D011968:Receptors, Histamine; D011969:Receptors, Histamine H1; D009538:Nicotine; D009116:Muscarine; D002927:Cimetidine; D006632:Histamine; D011899:Ranitidine; D002974:Clemastine; D002118:Calcium; D009638:Norepinephrine