Prevention of the TPA-mediated down-regulation of protein kinase C.
Protein kinase C activity in lymphocytes was down-regulated upon exposure of the cells to 12-0-tetradecanoylphorbol-13 acetate. This down-regulation was prevented by preincubating the cells with sphingosine, a reported protein kinase C inhibitor. Two other protein kinase C inhibitors, palmitoylcarnitine and phloretin, were ineffective in preventing down-regulation by the phorbol ester.
Grove
D S
DS
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Mastro
A M
AM
D000818:Animals; D002417:Cattle; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D010172:Palmitoylcarnitine / Q000494:pharmacology; D010693:Phloretin / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013110:Sphingosine / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010172:Palmitoylcarnitine; D011493:Protein Kinase C; D013110:Sphingosine; D013755:Tetradecanoylphorbol Acetate; D010693:Phloretin