Effects of non-ionic and anionic detergents on lipid-associated tissue ferritin.
Lipid-associated ferritin from homogenates of guinea pig liver is released from its conjugate(s) by incubation with the non-ionic detergents Triton X-100 and Nonidet P-40 but not by incubation with the anionic detergent deoxycholate. The amount of lipid-associated ferritin released from its conjugate(s) depends on the concentration of the non-ionic detergents. At a final non-ionic detergent concentration of about 20 g/L, all lipid-associated ferritin is released from its conjugate(s) in a liver homogenate. The amount released is identical with the amount of the lipid-associated ferritin obtained by extraction of the same liver homogenate with a mixture of butanol and diisopropyl ether.
Cham
B E
BE
Department of Medicine, University of Queensland, Royal Brisbane Hospital, Australia.
Roeser
P
P
Nikles
A
A
D000818:Animals; D000838:Anions; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D005293:Ferritins / Q000378:metabolism*; D006168:Guinea Pigs; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology*
D000838:Anions; D003902:Detergents; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; D005293:Ferritins; C010615:Nonidet P-40