title: Dot-immunobinding assay as an accurate and versatile technique for the quantification of human IgG subclasses.
abstract: A dot-immunobinding assay on nitrocellulose membranes has been developed for the quantification of human IgG subclasses using subclass-specific monoclonal antibodies. The advantages of this technique can be summarized as follows: (1) possibility of rapid semi-quantitative evaluation and/or precise quantitation from the same dot-pattern; (2) simple procedure with very good reproducibility; (3) sensitivity for nanogram concentrations of individual subclasses, therefore applicable not only to serum but also to other body fluids with a low content of IgG; (4) very small amounts of test material needed; (5) very good correlation of results with other techniques (ELISA, radial immunodiffusion) but without some of the inherent problems of the latter methods.
authors.affiliation: Department of Pediatrics, University Hospital, Leiden, The Netherlands.
authors.forename: C M
authors.initials: CM
authors.lastname: Jol-Van der Zijde
authors.forename: J
authors.initials: J
authors.lastname: Labadie
authors.forename: A
authors.initials: A
authors.lastname: Vlug
authors.forename: J
authors.initials: J
authors.lastname: Radl
authors.forename: J M
authors.initials: JM
authors.lastname: Vossen
authors.forename: M J
authors.initials: MJ
authors.lastname: Van Tol
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003101:Collodion; D003720:Densitometry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification / Q000592:standards; D010209:Paper; D011863:Radioimmunoassay; D012015:Reference Standards
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D003101:Collodion