title: Expression of c-myc during differentiation of the human teratocarcinoma cell line Tera-2.
abstract: The quantity of c-myc mRNA was measured during the retinoic-acid-induced differentiation of the pluripotent human teratoma cell line, Tera-2 cl. 13. As the cells were exposed to retinoic acid for longer periods of time the duration of the cell cycle progressively increased (measured by the rate of S phase entry) until the cells were effectively quiescent and expressed characteristic differentiation markers. Under these circumstances steady-state levels of c-myc expression increased by up to 1.6-fold with respect to rapidly growing undifferentiated cells. Southern blot analysis of the c-myc genes in Tera-2 indicated no major rearrangement or amplification in the cell line.
authors.affiliation: Department of Zoology, University of Oxford, UK.
authors.forename: P N
authors.initials: PN
authors.lastname: Schofield
authors.forename: W
authors.initials: W
authors.lastname: Engstrom
authors.forename: A J
authors.initials: AJ
authors.lastname: Lee
authors.forename: C
authors.initials: C
authors.lastname: Biddle
authors.forename: C F
authors.initials: CF
authors.lastname: Graham
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D014212:Tretinoin