Separations of flavonoids and alkaloids in medicinal herbs by high-speed counter-current chromatography.
Counter-current chromatography is a new liquid-liquid partition chromatography without using solid support. Recently, the technique has been remarkably improved in both partition efficiency and separation time. In this paper the capability of this high-speed counter-current chromatography was demonstrated on separation of two sets of samples obtained from medicinal herbs: a synthetic mixture of 3'-hydroxygenkwanin, luteolin and apigenin was separated on a two-phase solvent system composed of chloroform-methanol-water (4:3:2, v/v/v) and a crude ethanol extract from Anisodus tangulicus (Maxin) Pasch on chloroform-0.07 M sodium phosphate (pH 6.4) (1:1, v/v). In the light of chromatograms obtained from these samples, advantages of high-speed counter-current chromatography over other chromatographic methods were discussed in terms of partition efficiency, peak resolution, separation time, sample loading capacity, etc.
Zhang
T Y
TY
Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
Cai
D G
DG
Ito
Y
Y
D000470:Alkaloids / Q000302:isolation & purification*; D001285:Atropine / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000302:isolation & purification*; D019684:Magnoliopsida / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D010946:Plants, Medicinal / Q000032:analysis*; D012601:Scopolamine / Q000302:isolation & purification; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D005419:Flavonoids; D010936:Plant Extracts; D012997:Solvents; D001285:Atropine; D012601:Scopolamine