title: Immunoadsorbent purification of carcinoembryonic antigen using a monoclonal antibody: a direct comparison with a conventional method.
abstract: Carcinoembryonic antigen (CEA) has been extracted and purified by two different methods from a single aqueous homogenate of liver metastases from a primary adenocarcinoma of the colon, thus enabling direct comparison to be made between a conventional technique using gel filtration and a monoclonal antibody immunoadsorbent method. Yield, immunoreactivity and purity have been determined in both cases by direct weighing, enzyme-linked immunoadsorbent assay, radioimmunoassay and SDS-polyacrylamide gel electrophoresis. Reactivity of the antigen with monoclonal and polyclonal anti-CEA antibodies recognising different epitopes was analysed by Western blotting. There appears to be no significant difference in immunoreactivity or purity by these criteria, but the immunoadsorbent method gave a higher yield of CEA for far less expenditure of time and effort. A variant of CEA with a lower molecular weight was also identified in both preparations.
authors.affiliation: Oncology Research, Memorial University, St. John's, Newfoundland, Canada.
authors.forename: C H
authors.initials: CH
authors.lastname: Ford
authors.forename: F
authors.initials: F
authors.lastname: Macdonald
authors.forename: J A
authors.initials: JA
authors.lastname: Griffin
authors.forename: P
authors.initials: P
authors.lastname: Life
authors.forename: S E
authors.initials: SE
authors.lastname: Bartlett
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000556:secondary
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen