Botulinum C2 toxin ADP-ribosylates cytoplasmic beta/gamma-actin in arginine 177.
Isolated cytoplasmic actin of human platelet and pig liver actin, but not rabbit skeletal muscle actin, was ADP-ribosylated by botulinum C2 toxin in the presence of [32P]NAD. Tryptic digestion of the [32P]ADP-ribosylated platelet actin generated two labeled peptides: a soluble peptide covering residues 174-183 and an insoluble fragment containing residues 148-183. Further digestion of these two peptides with thermolysin yielded the same radioactive peptide, which was in both cases peptide 175-177. Amino acid sequence analysis of peptides 174-183 and 175-177 located the ADP-ribosylation on Arg177.
Vandekerckhove
J
J
Laboratorium voor Genetika, Gent, Belgium.
Schering
B
B
Bärmann
M
M
Aktories
K
K
D000199:Actins / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine; D001905:Botulinum Toxins / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D010449:Peptide Mapping; D011817:Rabbits; D013552:Swine
D000199:Actins; D000246:Adenosine Diphosphate Ribose; D001120:Arginine; D001905:Botulinum Toxins