Influence of single and double immunomagnetic depletion on the hemopoietic capacity of marrow in patients with advanced neuroblastoma submitted to autologous bone marrow transplantation.
Eighteen marrows from 16 patients with advanced neuroblastoma were submitted to a single or double immunomagnetic depletion procedure to purge them of tumor cells. Analysis of results showed that the CFU-GM recoveries were 59% +/- 16 (SE) and 33% +/- 16 after one or two cycles of incubation with microspheres. This led to yields of 7.8 x 10(4) and 4.1 x 10(4) CFU-GM available per kg body weight, respectively. Successful engraftment was demonstrated in all evaluable patients. However, some patients had delayed hematologic recoveries, particularly for platelets.
Lopez
M
M
INSERM U 76, CNTS Institut, Paris, France.
Zucker
J M
JM
Urresola
R
R
Douay
L
L
Quintana
E
E
Kemshead
J
J
Gorin
N C
NC
Vilcoq
J R
JR
D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002469:Cell Separation / Q000379:methods; D003114:Colony-Forming Units Assay; D006098:Granulocytes; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D007158:Immunologic Techniques; D007963:Leukocytes, Mononuclear; D008264:Macrophages; D008280:Magnetics; D009447:Neuroblastoma / Q000473:pathology / Q000628:therapy*; D014182:Transplantation, Autologous
D000911:Antibodies, Monoclonal