Markers of viral replication in patients with chronic hepatitis B virus infection.
Serum samples from 56 patients with biopsy-proven chronic B viral hepatitis without superimposed delta hepatitis were analyzed for the various markers of viral replication, including serum hepatitis B e Ag (HBeAg), hepatitis B virus deoxyribonucleic acid (HBV-DNA), and hepatitis B core antigen (HBcAg) in the liver tissues. Twenty-seven patients had persistent viral hepatitis (PH) and 29 patients had chronic active hepatitis (CAH) with or without cirrhosis. HBV-DNA was identified in the sera of 81% of patients with PH and 60% of patients with CAH. Significantly higher levels of HBV-DNA were found in patients with PH than in those with CAH. Both HBeAg in serum and HBcAg in liver correlated positively with serum HBV-DNA. Nine patients had serum HBV-DNA in the absence of HBeAg (four had anti-HBe), and seven of these nine patients had stainable HBcAg in the liver (two did not have staining). None of these patients had hepatic HBcAg in the absence of serum HBV-DNA. When these patients were stratified according to their epidemiologic background, serum HBV-DNA was present in a significantly higher number of male homosexuals than in any other groups. This was unrelated to their status of human immunodeficiency viral serology.
Govindarajan
S
S
University of Southern California Liver Unit, Department of Pathology and Medicine, Rancho Los Amigos Medical Center, Downey 90242.
Fong
T L
TL
Valinluck
B
B
Edwards
V
V
Redeker
A G
AG
D002908:Chronic Disease; D004247:DNA / Q000097:blood*; D006679:HIV Seropositivity / Q000150:complications; D006509:Hepatitis B / Q000097:blood / Q000150:complications / Q000276:immunology / Q000382:microbiology*; D006512:Hepatitis B Core Antigens / Q000032:analysis*; D006513:Hepatitis B e Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000276:immunology; D008297:Male; D014779:Virus Replication
D006512:Hepatitis B Core Antigens; D006513:Hepatitis B e Antigens; D004247:DNA