Factors affecting the acceptance of deoxyguanosine-treated thymus allografts.
dGuo-treated mouse embryo thymus lobes depleted of lymphoid and dendritic cells are not rejected by normal allogeneic mice despite expression of class I and class II major histocompatibility (MHC) antigens on thymic epithelial cells. Our results suggest that host mice are not tolerant to donor antigens, but treated lobes survive because thymic epithelial cells are not immunogenic. Multiple grafts of dGuo-treated lobes are rejected perhaps because numbers of residual dendritic cells reach a threshold necessary for priming. Mice previously primed by injection of spleen cells reject dGuo-treated thymus lobes, and crossreactions between major and minor histocompatibility antigens can be demonstrated. The acceptance of dGuo-treated thymus allografts by normal mice provides a system for investigating the requirements for priming.
Buckley
G
G
Department of Anatomy, University of Birmingham Medical School, England.
Benson
M T
MT
Jenkinson
E J
EJ
Owen
J J
JJ
D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects; D003849:Deoxyguanosine / Q000494:pharmacology*; D004848:Epithelium / Q000276:immunology; D005260:Female; D006085:Graft Survival / Q000187:drug effects*; D006183:H-2 Antigens / Q000276:immunology; D008221:Lymphoid Tissue / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D009924:Organ Culture Techniques; D013154:Spleen / Q000637:transplantation; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology / Q000637:transplantation*; D014184:Transplantation, Homologous
D006183:H-2 Antigens; D003849:Deoxyguanosine