Collisional spectroscopy as a screening procedure for the determination of 2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole from acid hydrolysis of B-poly(L-lysine) and B-albumin.
The direct determination of 2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole (FFI), present in the acid hydrolysis products of B-poly(L-lysine) and B-albumin and which appears to be a key intermediate in the physicochemical changes occurring during the incubation of protein with glucose, has been carried out by collisional spectroscopy, using a commercial double-focusing, reverse-geometry mass spectrometer and without any sample derivatization and chromatographic separation procedures.
Pelli
B
B
CNR, Area di Ricerca, Padova, Italy.
Sturaro
A
A
Traldi
P
P
Lapolla
A
A
Poli
T
T
Fedele
D
D
Crepaldi
G
G
D000418:Albumins; D003116:Color; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007093:Imidazoles / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D011107:Polylysine
D000418:Albumins; D007093:Imidazoles; D011107:Polylysine; C041306:2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole