Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles.
DNA enriched for supercoiled plasmids prepared from the 3 micron plasmid-enriched, [psi+], [2 micron degrees] strain 6-1G-P188 and from the [2 micron+] [psi+] strain LL20 can be used to transform a psi- recipient strain to psi+. Fractionation of the former preparation by electrophoresis showed that the 3 micron plasmid band contained the transforming activity.
Dai
H
H
Institute of Botany, Academia Sinica, Taipei, Republic of China.
Tsay
S H
SH
Lund
P M
PM
Cox
B S
BS
D004271:DNA, Fungal / Q000235:genetics; D004278:DNA, Superhelical / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal; D004278:DNA, Superhelical