Formaldehyde adducts of glutathione. Structure elucidation by two-dimensional n.m.r. spectroscopy and fast-atom-bombardment tandem mass spectrometry.
Aqueous mixtures of formaldehyde and glutathione react to form a variety of cyclized adducts in addition to S-hydroxymethylglutathione. The adducts are in labile equilibrium with each other and are not readily separated. The structures of two of the other major adducts were determined by concerted application of 13C-1H two-dimensional chemical-shift correlation, fast-atom-bombardment mass spectrometry and tandem mass spectrometry to the adduct mixtures in aqueous solution.
Naylor
S
S
University of Cambridge Chemical Laboratory, U.K.
Mason
R P
RP
Sanders
J K
JK
Williams
D H
DH
Moneti
G
G
D055598:Chemical Phenomena; D002621:Chemistry; D005557:Formaldehyde; D005978:Glutathione; D007477:Ions; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008970:Molecular Weight
D007477:Ions; D005557:Formaldehyde; D005978:Glutathione