Nonpermissivity of human peripheral blood lymphocytes to adenovirus type 2 infection.
The capacity of freshly explanted human peripheral blood lymphocytes (PBL) to support the replication of human adenovirus type 2 (Ad2) was investigated. Unlike other types of human cells, PBL were found to be highly nonpermissive. Ad2 adsorbed 30 to 40% of both T and non-T cells. Virus uncoating was very slow and inefficient, resulting in a 40-fold reduction compared with HEp-2 cells. On a population basis, viral DNA synthesis was reduced 460-fold and infectious virus production was reduced 10(6)-fold. Only 0.35% of PBL produced infectious centers, yielding 0.8 PFU per infected cell. Phytohemagglutinin stimulation increased DNA synthesis 23-fold, infectious centers 11-fold, and virus yield 14-fold. We conclude that resting human PBL are highly nonpermissive to Ad2 infection and that phytohemagglutinin can only marginally lift this nonpermissiveness.
Horvath
J
J
Département de Microbiologie, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
Weber
J M
JM
D000260:Adenoviruses, Human / Q000254:growth & development*; D002213:Capsid / Q000378:metabolism; D002451:Cell Compartmentation; D002467:Cell Nucleus; D004279:DNA, Viral / Q000096:biosynthesis; D008214:Lymphocytes / Q000382:microbiology*; D009024:Morphogenesis; D010948:Viral Plaque Assay; D014779:Virus Replication
D004279:DNA, Viral