Expression of epidermal growth factor receptor proto-oncogene mRNA in regenerating rat liver.
The expression of EGF receptor mRNAs in regenerating rat liver was measured using two nonoverlapping cDNA probes for the human gene from a highly conserved region. These probes (pE7 and pE62) both hybridized to RNA species of 10 and 6 kb. The 10 and 6 kb RNA species were shown to decrease in the first 12 hours after partial hepatectomy. However, significant increases above control levels were noted at 24h and 72h. The level of alpha-actin mRNA increased as has been previously reported. These results suggest that a transcriptional and/or a posttranscriptional regulatory mechanism exists in regenerating rat liver with respect to EGF receptor gene expression.
Johnson
A C
AC
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Garfield
S H
SH
Merlino
G T
GT
Pastan
I
I
D000199:Actins / Q000235:genetics; D000818:Animals; D004247:DNA / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics*; D006498:Hepatectomy; D007700:Kinetics; D008115:Liver Regeneration; D008297:Male; D009693:Nucleic Acid Hybridization; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats
D000199:Actins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D004247:DNA; D066246:ErbB Receptors