Brain arginine vasotocin concentrations related to sexual behaviors and hydromineral balance in an amphibian.
Arginine vasotocin (AVT), a potent stimulator of sexual behaviors and regulator of hydromineral balance in male rough-skinned newts (Taricha granulosa), was measured in 11 brain areas using microdissection and radioimmunoassay procedures. A 60-min test for sexual behaviors was used to segregate males into two groups: sexually responsive (initiated amplectic clasping behaviors) and sexually unresponsive (exhibited no sexual behaviors). Compared to sexually unresponsive males, sexually responsive males had significantly higher concentrations of immunoreactive (ir) AVT in the dorsal preoptic area, optic tectum, ventral infundibular nucleus, and cerebrospinal fluid. These results provide evidence for a behavioral action of endogenous AVT in T. granulosa. In another study, irAVT was measured in normal males (control newts maintained in water) and males that were dehydrated for 6 hr. Compared to normal males, dehydrated males had significantly lower concentrations of irAVT in the ventral preoptic area, but not in the other 10 areas of the brain. That different brain areas are associated with sexual behaviors and hydromineral balance suggests that there are some neuroanatomical separations between the behavioral and hydromineral aspects of the vasotocinergic system in this amphibian.
Zoeller
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Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland 20892.
Moore
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D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000032:analysis; D001923:Brain Chemistry; D003345:Corticosterone / Q000097:blood; D003681:Dehydration / Q000503:physiopathology; D008297:Male; D011301:Preoptic Area / Q000032:analysis; D012455:Salamandridae; D012726:Sexual Behavior, Animal / Q000502:physiology*; D013477:Superior Colliculi / Q000032:analysis; D014668:Vasotocin / Q000032:analysis* / Q000134:cerebrospinal fluid
D014668:Vasotocin; D003345:Corticosterone