Qualitative evidence for expression of Klebsiella pneumoniae nif in Pseudomonas putida.
Pseudomonas putida MT20-3 carrying the Klebsiella pneumoniae nif plasmids pRD1 or pMF250 showed highly O2-sensitive aerobic acetylene reduction on low-N pyruvate or glucose agar. This finding confirms unequivocally that K. pneumoniae nif can be expressed in an obligate aerobe.
Postgate
J R
JR
AFRC Unit of Nitrogen Fixation, University of Sussex, UK.
Kent
H M
HM
D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics*; D009586:Nitrogen Fixation / Q000235:genetics*; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*