Suppression of gastric ulcer induced by stress and HCL-ethanol by intravenously administered metallothionein-II.
A metallothionein isoform metallothionein-II was isolated from the livers of zinc acetate-treated rats. Metallothionein-II, which showed a single band on polyacrylamide gel electrophoresis, was subjected to two kinds of anti-ulcer screening systems. It was shown that intravenously administered metallothionein-II suppressed the formation of rat water-immersion stress- and HCl-ethanol-induced gastric ulcer significantly. The effect may partly be derived from the zinc contained in the metallothionein-II. However, the effect of metallothionein-II was much stronger than that of an equivalent mole of zinc. Apparently, metallothionein-II had an anti-ulcerogenic activity not based on the effect of intrinsic zinc.
Mimura
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Tsujikawa
K
K
Yasuda
N
N
Nakajima
H
H
Haruyama
M
M
Ohmura
T
T
Okabe
M
M
D000085:Acetates / Q000627:therapeutic use; D019342:Acetic Acid; D000818:Animals; D002927:Cimetidine / Q000627:therapeutic use; D000431:Ethanol; D006851:Hydrochloric Acid; D008297:Male; D008668:Metallothionein / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control* / Q000523:psychology; D013315:Stress, Psychological / Q000503:physiopathology*
D000085:Acetates; D000431:Ethanol; D002927:Cimetidine; D008668:Metallothionein; D019342:Acetic Acid; D006851:Hydrochloric Acid