Drug test chamber: a titanium implant for administration of biochemical agents to a standardized bone callus in situ.
A titanium implant in which a conduit is gradually filled with ingrowing bone (the Bone Harvest Chamber) has been modified to allow continuous local treatment of the conduit tissue with biochemical agents. Implants were inserted bilaterally in rabbit tibiae. The tissue content of the bone ingrowth conduits was studied with histology, 99mTc-MDP scintimetry and measurements of total calcium content. Bone was formed in the conduit by endochondral formation starting at both ends and continuing until fusion in the middle. After 2 weeks the bone had not yet met in the middle where fibrous tissue was seen. In eight animals 3H-proline was applied via one of the chambers, with the contralateral chamber as a saline-treated control. The collagen of the harvested tissue from the 3H-proline treated side had a 3H-hydroxyproline content 1000 times greater than had the control side. The 'drug test chamber' makes possible the study of local effects of drugs on healing of mature bone in vivo.
Aspenberg
P
P
Department of Orthopaedics, University of Lund, Sweden.
Albrektsson
T
T
Lohmander
L S
LS
Thorngren
K G
KG
D000818:Animals; D002146:Bony Callus / Q000033:anatomy & histology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D007260:Infusion Pumps; D011392:Proline / Q000008:administration & dosage / Q000378:metabolism; D019736:Prostheses and Implants; D011817:Rabbits; D014025:Titanium
D011392:Proline; D014025:Titanium; D006909:Hydroxyproline; D002118:Calcium