title: Phenotypic suppression and nuclear accommodation of the mit- oxi1-V25 mutation in isolated yeast mitochondria.
abstract: Phenotypic suppression by the antibiotic, paromomycin, of the mitochondrial oxi1- -V25 mutation, a mutation which arrests by premature ochre codon the synthesis of the cox II subunit, was studied in isolated yeast mitochondria competent in translation. This antibiotic is known to suppress the mutation in vivo (Dujardin et al. 1984) and allowed in vitro, at concentrations of 20-1100 micrograms per ml. the synthesis of the cox II subunit. This strongly suggests that phenotypic suppression of mit- mutations is due to the direct action of paromomycin on mitochondrial ribosomes. The effect of paromomycin bears a resemblance to the function of the omnipotent nuclear suppressor mutation R705. The nuclear suppression was expressed in isolated mitochondria; suppressor mutation influenced the structure of the mitoribosome. Therefore, it appears that mitoribosomes are indeed the common target in the phenotypical and genetic nuclear suppression of the oxi1-V25 mutation.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
authors.forename: W
authors.initials: W
authors.lastname: Zagórski
authors.forename: M
authors.initials: M
authors.lastname: Boguta
authors.forename: M
authors.initials: M
authors.lastname: Mieszczak
authors.forename: M
authors.initials: M
authors.lastname: Claisse
authors.forename: B
authors.initials: B
authors.lastname: Guiard
authors.forename: A
authors.initials: A
authors.lastname: Spyridakis
authors.forename: P P
authors.initials: PP
authors.lastname: Slonimski
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005796:Genes; D005800:Genes, Fungal; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D012269:Ribosomal Proteins; D008715:Methionine