Characterization of a nonhemagglutinating mutant of canine parvovirus.
A nonhemagglutinating mutant of a 1978 isolate of canine parvovirus (CPV) was derived after repeated passages in the NLFK feline kidney cell line. The mutant CPV was antigenically indistinguishable from wild-type virus when tested with 82 monoclonal antibodies, and it replicated in cat and dog cell lines in culture. Sequences of the VP-1 and VP-2 genes revealed two nucleotide and two predicted amino acid sequence differences at 77 and 88 genome map units in the mutant compared to hemagglutinating viruses. One or both of those two mutations must determine the difference in the ability of the virus to agglutinate erythrocytes.
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James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
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D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002213:Capsid / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D004285:Dogs; D005814:Genes, Viral; D006387:Hemagglutination, Viral; D009154:Mutation; D010321:Parvoviridae / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins
D000956:Antigens, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins