The purification of four respiratory syncytial virus proteins and their evaluation as protective agents against experimental infection in BALB/c mice.
The fusion (F) glycoprotein, large glyco- (G) protein, phospho- (P) protein and 22K protein of respiratory syncytial (RS) virus A2 strain were purified by a combination of immunoaffinity adsorption and preparative SDS-PAGE. All four proteins elicited serum antibody in mice after repeated inoculation in adjuvant, although the magnitude of the response as measured by ELISA varied from mouse to mouse. The F protein generated neutralizing antibodies in only 50% of the mice determined to be seropositive by ELISA. The G protein also induced neutralizing antibodies but in this instance neutralization tests and ELISA titres were more closely correlated. No neutralizing activity was detected in mice immunized with the P or 22K proteins although all produced antibody detectable by ELISA. Mice immunized with either the F or the G protein were found to be protected against subsequent RS virus challenge, whether they had developed neutralizing antibody or not. Mice inoculated with the P or 22K proteins were not protected.
Routledge
E G
EG
Department of Virology, University of Newcastle upon Tyne, U.K.
Willcocks
M M
MM
Samson
A C
AC
Morgan
L
L
Scott
R
R
Anderson
J J
JJ
Toms
G L
GL
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010750:Phosphoproteins / Q000276:immunology / Q000302:isolation & purification; D012136:Respiratory Syncytial Viruses / Q000032:analysis* / Q000276:immunology; D010253:Respirovirus Infections / Q000517:prevention & control*; D014759:Viral Envelope Proteins / Q000276:immunology / Q000302:isolation & purification; D014760:Viral Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D006023:Glycoproteins; D010750:Phosphoproteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D014765:Viral Vaccines