Heteroclitic antibodies: differences in fine specificities between monoclonal antibodies directed against dinitrophenyl and trinitrophenyl haptens.
The inhibition of binding of monoclonal antibodies by different haptens was studied using the 50% antibody binding assay. The binding of antidinitrophenyl and antitrinitrophenyl antibodies to dinitrophenylated or trinitrophenylated bovine serum albumin could be inhibited by monovalent dinitrophenyl or trinitrophenyl epsilon-aminocaproic acid. Some of the antibodies could be inhibited to a greater degree with the cross-reacting haptens than with the haptens homologous to the immunizing antigen, therefore these antibodies were heteroclitic.
Furusawa
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S
New York University Medical School, Department of Pathology, N.Y.
Ovary
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Z
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D003429:Cross Reactions; D004136:Dinitrobenzenes / Q000276:immunology*; D005260:Female; D006241:Haptens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009578:Nitrobenzenes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D004136:Dinitrobenzenes; D006241:Haptens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes