Effect of some thiol reagents on erythrocyte adenosine deaminase (ADA) activity.
The effect of three thiol reagents on erythrocyte adenosine deaminase (ADA) activity has been studied. Oxidized glutathione and iodoacetate do not alter ADA activity, while the treatment with p-chloromercuribenzoate at similar concentrations results in a reduction of enzymatic activity which is statistically significant only for ADA 1, but not ADA 2-1 phenotype haemolysates.
Corbo
R M
RM
Department of Genetics and Molecular Biology, La Sapienza University, Rome, Italy.
Scacchi
R
R
Mantuano
E
E
D000243:Adenosine Deaminase / Q000097:blood*; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D007527:Isoenzymes / Q000097:blood*; D007700:Kinetics; D009700:Nucleoside Deaminases / Q000097:blood*; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D007461:Iodoacetates; D007527:Isoenzymes; D013439:Sulfhydryl Reagents; D020245:p-Chloromercuribenzoic Acid; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D005978:Glutathione; D019807:Iodoacetic Acid