Elicitor-induced tyrosine decarboxylase in berberine-synthesizing suspension cultures of Thalictrum rugosum.
Tyrosine decarboxylase (EC 4.1.1.25) was induced in suspension cultures of Thalictrum rugosum by treatment with a yeast glucan elicitor. Maximum induction was observed at a carbohydrate concentration of 0.4 mg/g fresh weight of cells and maximum enzyme activity was reached 20 h after addition of elicitor. The enzyme was inducible in late exponential and early stationary growth phases. A good correlation between induced tyrosine decarboxylase activity and berberine biosynthesis has been established. It is suggested that tyrosine decarboxylase may be a key enzyme between primary and secondary metabolisms in the biosynthesis of norlaudanosoline-derived alkaloids.
Gügler
K
K
Institute of Biotechnology, ETH-Hönggerberg, Zürich, Switzerland.
Funk
C
C
Brodelius
P
P
D001599:Berberine / Q000096:biosynthesis*; D001600:Berberine Alkaloids / Q000096:biosynthesis*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D007700:Kinetics; D010944:Plants / Q000201:enzymology*; D014445:Tyrosine Decarboxylase / Q000096:biosynthesis*
D001600:Berberine Alkaloids; D001599:Berberine; D014445:Tyrosine Decarboxylase