Assessment of L-ascorbic acid requirement for prolonged survival in ODS rats and their susceptibility to urinary bladder carcinogenesis by N-butyl-N-(4-hydroxybutyl)nitrosamine.
Assessment of L-ascorbic acid requirement for prolonged survival in ODS (genotype: od/od) rats and their susceptibility to urinary bladder carcinogenesis by N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) were examined. In ODS rats without L-ascorbic acid synthesizing ability, the 50 ppm dietary total ascorbic acid (TAA) was insufficient to survive for 4 weeks, the 250 ppm dietary TAA was sufficient to survive for 36 weeks. In examination of BBN treatment, ODS rats--although showing a lower availability of TAA than the heterozygotes (+/od) and normal (+/+) rats with L-ascorbic acid synthesizing ability--were equally susceptible to bladder carcinogenesis.
Mori
S
S
First Department of Pathology, Nagoya City University, Medical School, Japan.
Takeuchi
Y
Y
Toyama
M
M
Makino
S
S
Harauchi
T
T
Kurata
Y
Y
Fukushima
S
S
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage*; D001206:Ascorbic Acid Deficiency / Q000150:complications*; D002085:Butylhydroxybutylnitrosamine / Q000378:metabolism; D008297:Male; D051381:Rats; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced*
D002085:Butylhydroxybutylnitrosamine; D001205:Ascorbic Acid