Triiodothyronine (T3) induces neurite formation and increases synthesis of a protein related to MAP 1B in cultured cells of neuronal origin.
Neuroblastoma (N2A) cells were found to develop axon-like neurite extensions when grown in the presence of triiodothyronine (T3), while C6 cells (of glial origin) did not. Analysis of radiolabelled protein synthesis showed that, in N2A only, T3 increased the synthesis of a polypeptide corresponding in electrophoretic mobility to the microtubule-associated protein MAP 1B. Immunoblotting of total cell proteins with a monoclonal antibody confirmed that this polypeptide was immunologically related to MAP 1B. Further studies using indirect immunofluorescence with monoclonal antibodies against both tubulin and MAP 1B showed that both antigens were present in neurites. Taken together, these results suggest that T3 may control maturation of neural tissue via effects on the microtubule-associated proteins in cells of neuronal origin.
Hargreaves
A
A
Centro de Biologia Molecular, U.A.M., Spain.
Yusta
B
B
Aranda
A
A
Avila
J
J
Pascual
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000276:immunology; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D051381:Rats; D014284:Triiodothyronine / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D014404:Tubulin; D014284:Triiodothyronine