Meal-stimulated release of methionine-enkephalin into the canine jejunal lumen.
Application of enkephalins to the luminal surface of the bowel augments intestinal absorption. However, to date, endogenous enkephalins have not been demonstrated within intestinal luminal fluid. To determine whether enkephalins are present in the intestinal lumen, five adult dogs had 25-cm chronic jejunal Thiry-Vella loops constructed. Dogs were studied in the awake, fasted state. Jejunal loops were perfused with isoosmotic, neutral Krebs buffer containing protease inhibitors. After basal sampling, the dogs received a high fat meat meal. Collections were made during the meal and for 60 min postprandially. Luminal met-enkephalin levels were determined by radioimmunoassay and confirmed by HPLC. HPLC separation of luminal samples demonstrated two immunoreactive peaks which co-eluted with pure met-enkephalin and met-enkephalin-sulfoxide. Basal met-enkephalin outputs averaged 52 +/- 13 ng/min. The meal significantly increased mean luminal met-enkephalin output to 137 +/- 71 ng/min. During the initial 20-min postprandial period, output remained elevated (180 +/- 73 ng/min), after which it returned to basal levels. We conclude that met-enkephalin is present in the jejunal lumen, and that luminal release of this opioid is augmented by a meal.
Money
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Department of Surgery, State University of New York Health Science Center, Brooklyn 11203.
Petroianu
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A
Gintzler
A R
AR
Jaffe
B M
BM
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D004285:Dogs; D004744:Enkephalin, Methionine / Q000032:analysis / Q000378:metabolism*; D005260:Female; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism*; D010477:Perfusion; D011863:Radioimmunoassay; D013997:Time Factors
D004744:Enkephalin, Methionine