Monitoring of veterinary drug residues by a combination of continuous flow techniques and column-switching high-performance liquid chromatography. I. Sulphonamides in egg, meat and milk using post-column derivatization with dimethylaminobenzaldehyde.
By using a combination of a low-pressure continuous flow module and a column-switching high-performance liquid chromatographic system, procedures have been developed for the automated residue analysis of sulphonamides and selected other drugs in meat, egg and milk. Aqueous extracts are purified by on-line dialysis and subsequent trace enrichment on a short column containing silica-based or polymer material. After backflush to the analytical column, detection is performed either at 280 nm or, after post-column derivatization with p-dimethylaminobenzaldehyde, at 450 nm. With these procedures the fully automated determination of both polar and apolar sulphonamides and dapsone above 5-20 micrograms/kg is possible. Coefficients of variation are 4-10%, recoveries compared to standards are 85-90%. The methods developed were tested in routine veterinary drug control.
Aerts
M M
MM
State Institute for Quality Control of Agricultural Products, Wageningen, The Netherlands.
Beek
W M
WM
Brinkman
U A
UA
D000818:Animals; D001547:Benzaldehydes; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000032:analysis*
D001547:Benzaldehydes; D007202:Indicators and Reagents; D013449:Sulfonamides; C510241:p-dimethylaminobenzaldehyde