title: Biochemical and histopathologic comparison between blood and saline storage of canine veins.
abstract: Patency of vein grafts may be influenced by the medium in which they are stored temporarily. We compared saline solution vs blood on prostanoid production and maintenance of endothelium in canine veins after 1 hour of storage at 23 degrees C with 0.2 mg/ml of papaverine. Spontaneous and arachidonate-stimulated prostaglandin levels were measured by radioimmunoassay. Endothelial integrity was analyzed by light and electron microscopy. Prostaglandin production in blood vs that in saline solution was 1821 +/- 1264 and 1259 +/- 719 pg/cm2/min at control and 6705 +/- 3702 vs 6264 +/- 3409 pg/cm2/min, respectively, after stimulation. There were no statistically significant differences between the groups at any time point. Thromboxane levels were also indistinguishable between groups. Microscopy revealed 70% endothelial loss in blood vs 95% for saline solution. We conclude that endothelial preservation is enhanced by blood storage, that the medial layer produces substantial amounts of prostacyclin, and that additional storage solutions need to be investigated.
authors.affiliation: Department of Surgery, Brooklyn Veterans Administration Medical Center.
authors.forename: E L
authors.initials: EL
authors.lastname: Hoover
authors.forename: M
authors.initials: M
authors.lastname: Ross
authors.forename: K
authors.initials: K
authors.lastname: Fani
authors.forename: H
authors.initials: H
authors.lastname: Webb
authors.forename: D
authors.initials: D
authors.lastname: Kirshy
authors.forename: F
authors.initials: F
authors.lastname: DiMaio
authors.forename: A
authors.initials: A
authors.lastname: Ketosugbo
authors.forename: A F
authors.initials: AF
authors.lastname: Debons
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001769:Blood; D001807:Blood Vessel Prosthesis; D004285:Dogs; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure; D007601:Jugular Veins; D008297:Male; D012965:Sodium Chloride; D013929:Thromboxane B2 / Q000378:metabolism; D014021:Tissue Preservation / Q000379:methods*; D014654:Vascular Patency
chemical_list: D012965:Sodium Chloride; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha