The half-lives of serum immunoglobulins in adult mice.
We determined the half-lives of several sets of murine monoclonal antibodies spanning all immunoglobulin isotypes in the serum. The antibodies in each set possess the same V region. With this approach, the differences in half-life observed between the different isotypes are independent of the V region carried by the monoclonal antibodies and therefore must relate to each other in the same way as the half-lives of each class of serum immunoglobulins. The half-life of a monoclonal antibody of the gamma 2a isotype is identical to the average half-life of serum IgG2a as previously determined (6-8 days; P. Vieira and K. Rajewsky, Eur. J. Immunol. 1986. 16:871). Therefore, the half-lives determined with monoclonal antibodies possessing the same V region represent the half-life of the serum immunoglobulins. In this way we calculated the half-life of IgM as 2 days, IgG3 and IgG1 as 6-8 days, IgG2b has a half-life of 4-6 days. IgE has a half-life of 12 h. A polymeric form of IgA was found to be eliminated from the serum with a half-life of 17-22 h.
Vieira
P
P
Institute for Genetics, University of Cologne, FRG.
Rajewsky
K
K
D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D005260:Female; D006207:Half-Life; D006241:Haptens / Q000276:immunology; D007074:Immunoglobulin G / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009596:Nitrophenols / Q000276:immunology; D010648:Phenylacetates
D000911:Antibodies, Monoclonal; D006241:Haptens; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes; D007136:Immunoglobulins; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid