title: Expression of human pancreatic secretory trypsin inhibitor in Saccharomyces cerevisiae.
abstract: Human pancreatic secretory trypsin inhibitor (PSTI) cDNA was expressed in Saccharomyces cerevisiae using the yeast acid phosphatase PHO5 promoter. The product encoded by the PSTI-coding cDNA was correctly processed in yeast cells, and the PSTI molecules were efficiently secreted into the medium. The amino acid composition and the N-terminal amino acid sequence of the secreted PSTI molecules were identical to those of the authentic PSTI polypeptides from human pancreas, and the product exhibited trypsin-inhibitory activity.
authors.affiliation: Institute for Molecular and Cellular Biology, Osaka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Izumoto
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Yoshida
authors.forename: N
authors.initials: N
authors.lastname: Kikuchi
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Matsubara
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D005800:Genes, Fungal; D006801:Humans; D010189:Pancreatic Juice / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000235:genetics*; D014361:Trypsin Inhibitors / Q000235:genetics*
chemical_list: D014361:Trypsin Inhibitors; D014359:Trypsin Inhibitor, Kazal Pancreatic; D000135:Acid Phosphatase