Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase.
The genome of the lipid-containing bacteriophage phi 6 contains three segments of double-stranded RNA. We determined the nucleotide sequence of cDNA derived from the largest RNA segment (L). This segment specifies the procapsid proteins necessary for transcription and replication of the phi 6 genome. The coding sequences of the four proteins on this segment were identified on the basis of size and the correlation of predicted N-terminal amino acid sequences with those found through analysis of isolated proteins. This report completes the sequence analysis of phi 6. This constitutes the first complete sequence of a double-stranded RNA genome virus.
Mindich
L
L
Department of Microbiology, Public Health Research Institute of the City of New York, Inc., New York.
Nemhauser
I
I
Gottlieb
P
P
Romantschuk
M
M
Carton
J
J
Frucht
S
S
Strassman
J
J
Bamford
D H
DH
Kalkkinen
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D012317:RNA Phages / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D014158:Transcription, Genetic; D014779:Virus Replication
D011498:Protein Precursors; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D004247:DNA; D012324:RNA-Dependent RNA Polymerase; D012321:DNA-Directed RNA Polymerases