Characterization of adsorption on the stationary phase using high-performance immunoaffinity chromatography.
Low-level adsorption on the stationary phase has been studied using immunochemical reagents. An immunoaffinity column has been evaluated using affinity-purified radioisotope-labeled monoclonal antibodies. Recovery experiments including continuous immunosorbent monitoring have been performed. Proper characterization of an immunoaffinity separation can result in the recovery of immunologically active material in high yield.
Nielsen
R G
RG
Department of Chemistry, University of Arizona, Tucson 85721.
Wilson
G S
GS
D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000032:analysis; D002021:Buffers; D002712:Chlorides / Q000032:analysis; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000032:analysis; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000032:analysis; D007202:Indicators and Reagents; D007454:Iodides / Q000032:analysis; D007457:Iodine Radioisotopes
D000911:Antibodies, Monoclonal; D002021:Buffers; D002712:Chlorides; D007074:Immunoglobulin G; D007164:Immunosorbents; D007202:Indicators and Reagents; D007454:Iodides; D007457:Iodine Radioisotopes; C009813:iodine monochloride