Expression of two cross-reactive idiotypes on mouse antibodies against bromelain-treated mouse erythrocytes.
Two cross-reactive anti-idiotype (Id) antibodies were previously prepared from sera of rabbits immunized with mouse monoclonal antibodies against bromelain-treated mouse erythrocytes (BrMRBC). Most of the anti-BrMRBC plaque-forming cells (PFC) were suppressed by either of the two anti-Id antibodies. The Id profiles of anti-BrMRBC PFC were almost identical among various cell populations in a strain, but different among various mouse strains. Mouse sera contained both of the Id-bearing immunoglobulins Ig, and a significant part of the Id-bearing Ig were eliminated by absorption with BrMRBC. Nude BALB/c mice were almost equal to normal BALB/c mice in the Id patterns of anti-BrMRBC PFC and in the concentrations of the Id-bearing Ig. The injections of anti-Id antibodies into suckling mice suppressed, specifically, the development of the B cells to produce the homologous Id-bearing Ig, but the injection of Id-bearing monoclonal antibodies barely affected Id expression. It is suggested that the two Id are encoded in germ-line genes of mice, and are expressed independently of each other and Id-anti-Id regulations by T cells or B cells.
Kawaguchi
S
S
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001963:Bromelains / Q000276:immunology*; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains
D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D001963:Bromelains