Analysis of cellular expression of gangliosides by gene transfection. I: GD3 expression in myc-transfected and transformed 3Y1 correlates with anchorage-independent growth activity.
Transfection of c-myc DNA into rat fibroblastic 3Y1 cell line resulted in the neosynthesis of GD3 ganglioside, as has been previously shown to occur after transfection of 3Y1 cells with adeno El (Nakakuma, H., Sanai, Y., Shiroki, K., and Nagai, Y. (1984) J. Biochem. 96, 1471-1480); in both cases, the products are expressed intranuclearly. Moreover, a clear correlation was identified between levels of GD3 expression and colony-forming activity (in soft agar) of myc-transformed 3Y1 cells, implying that GD3 plays some specific role in myc-induced transformation of 3Y1 cell line.
Nakaishi
H
H
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
Sanai
Y
Y
Shiroki
K
K
Nagai
Y
Y
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004622:Embryo, Mammalian; D005732:Gangliosides / Q000235:genetics* / Q000302:isolation & purification; D009857:Oncogenes; D051381:Rats; D014162:Transfection
D005732:Gangliosides; C026226:ganglioside, GD3