title: Induction of yeast DNA ligase genes in exponential and stationary phase cultures in response to DNA damaging agents.
abstract: UV-irradiation of stationary phase cells of Saccharomyces cerevisiae and Schizosaccharomyces pombe leads to a 9-fold and 90-fold increase in transcript levels from the respective DNA ligase genes CDC9 and CDC17, whereas exponential cells show only 3-fold and 2-fold increases. Induction of CDC9 after MMS treatment and gamma-irradiation was also observed by using a CDC9-lacZ translational fusion and assaying for beta-galactosidase. Surprisingly, irradiation of S. cerevisiae induces only a 50% increase in DNA ligase itself, probably reflecting the extremely high in vivo stability of the enzyme. The UV-induction of ligase may be part of a "fail-safe" mechanism which, together with the enzyme stability, ensures adequate supplies of this essential enzyme.
authors.affiliation: Laboratory of Cell Propagation, National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: A L
authors.initials: AL
authors.lastname: Johnson
authors.forename: D G
authors.initials: DG
authors.lastname: Barker
authors.forename: L H
authors.initials: LH
authors.lastname: Johnston
mesh_terms: D004249:DNA Damage; D011088:DNA Ligases / Q000096:biosynthesis / Q000235:genetics*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D005720:Gamma Rays; D005796:Genes / Q000187:drug effects / Q000528:radiation effects; D005800:Genes, Fungal / Q000187:drug effects / Q000528:radiation effects; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D011118:Polynucleotide Ligases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014466:Ultraviolet Rays
chemical_list: D008741:Methyl Methanesulfonate; D011088:DNA Ligases; D011118:Polynucleotide Ligases