Polyploidization of the BTC-32 cell line from Triatoma infestans (Hemiptera, Reduviidae).
The cytophotometric evaluation of the Feulgen-DNA content of the BTC-32 cells at passage 160 after 6 days of growth demonstrated that polyploidy is relatively frequent in this cell line. 4C values were assumed to pertain to diploid nuclei at the S or G2 phases but also to polyploid nuclei at the G1 phase. Polyploidy in 4C nuclei is assumed to be attained by endomitosis. However, there are morphological indications that polyploidization in cells with an 8C-128C Feulgen-DNA content could result from cellular and nuclear fusions, possibly mediated by viral particles present in the cell culture. Micronucleation was also frequent; it was assumed to be promoted by viral action or deficiency in some culture medium nutrient. These nuclear characteristics should be considered when using the BTC-32 cell line for monitoring the action of infective agents or their products.
Mello
M L
ML
Dept. of Cell Biology, UNICAMP, Campinas (SP), Brazil.
Pudney
M
M
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002871:Chromosome Banding; D004247:DNA / Q000032:analysis; D011123:Polyploidy; D014224:Triatoma / Q000235:genetics*; D014225:Triatominae / Q000235:genetics*
D004247:DNA