Human anti-alpha-galactosyl IgG reduces the lung colonization by murine MO4 cells.
The lung colonization of MO4 cells, a highly malignant murine cell line, is strongly reduced in syngeneic C3H/He mice by a prior incubation with anti-alpha-galactosyl antibody (alpha-Gal Ab), a natural IgG antibody present in high titers in all normal human sera and specifically recognizing Gal alpha(1----3) structures (alpha-D-galactopyranosyl; alpha-D-Galp). The protective effect is due to a binding of alpha-Gal Ab to alpha-D-Galp end groups of MO4 cells, inducing both an increase in their sequestration into the liver and the spleen and a decrease in their sequestration into the lung. The F(ab')2 fragments of this antibody also exhibit a protective effect by inhibiting the homing of MO4 cells into the lung, without modifying their accumulation into the spleen and the liver. Since both the antibody and the alpha-galactosidase pretreatments of MO4 cells block their subsequent attachment to murine laminin in vitro, we suggest that, in this model, the lung colonization may be dependent on the alpha-D-Galp end groups exposed on the surface of MO4 cells.
Castronovo
V
V
Department of Obstetrics and Gynecology, University of Liège, Belgium.
Foidart
J M
JM
Li Vecchi
M
M
Foidart
J B
JB
Bracke
M
M
Mareel
M
M
Mahieu
P
P
D000818:Animals; D005455:Fluorescent Antibody Technique; D005690:Galactose / Q000276:immunology*; D007074:Immunoglobulin G / Q000008:administration & dosage*; D007167:Immunotherapy; D007797:Laminin / Q000378:metabolism; D008175:Lung Neoplasms / Q000276:immunology / Q000517:prevention & control / Q000556:secondary*; D051379:Mice; D008809:Mice, Inbred C3H; D008856:Microscopy, Fluorescence; D009368:Neoplasm Transplantation
D007074:Immunoglobulin G; D007797:Laminin; D005690:Galactose