title: Binding of fibronectin, fibrinogen and type II collagen to streptococci isolated from bovine mastitis.
abstract: Binding of 125I-labelled fibronectin, fibrinogen and type II collagen to group B (S. agalactiae), group C (S. dysgalactiae and S zooepidemicus), group E (S. uberis) and nontypable streptococci isolated from bovine mastitis was studied. S. agalactiae and S. uberis were found to bind low levels of all three proteins, while S. zooepidemicus bound high levels. Binding of the proteins to S. dysgalactiae varied, i.e. fibronectin was high, fibrinogen moderate and collagen low. Nontypable strains showed moderate or low binding of all proteins. Both hydrophobic and hydrophilic strains were found to bind fibronectin. For S. dysgalactiae the specific fibronectin binding ranged from 70% to 10% and for S. zooepidemicus it was more than 80% and this binding was sensitive to papain treatment. The binding of 29K-fibronectin fragment to one S. dysgalactiae strain showed an affinity of KD = 2.6 x 10(-8) M and the number of binding sites per colony forming unit (CFU) was calculated at 11,000.
authors.affiliation: Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: W
authors.initials: W
authors.lastname: Mamo
authors.forename: G
authors.initials: G
authors.lastname: Fröman
authors.forename: A
authors.initials: A
authors.lastname: Sundås
authors.forename: T
authors.initials: T
authors.lastname: Wadström
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D007700:Kinetics; D008414:Mastitis, Bovine / Q000382:microbiology*; D011485:Protein Binding; D013290:Streptococcal Infections / Q000662:veterinary*; D013291:Streptococcus / Q000302:isolation & purification / Q000378:metabolism*; D013292:Streptococcus agalactiae / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D005353:Fibronectins; D005340:Fibrinogen; D003094:Collagen