[Demonstration of thrombocyte membrane proteins with monoclonal antibodies by a flow cytometry bioassay].
Formation of a hemostatic plug is triggered by platelets. Platelet function (e.g. adhesion, aggregation) depends essentially on membrane bound receptor proteins. Conventional chromatographic analysis of these glycoprotein macromolecules is difficult and not appropriate for diagnostic routine. In combination of cytoflowmetric single cell analysis with monoclonal staining we developed a bio-assay for qualitative and semi-quantitative analysis of glycoprotein IB and IIB/IIIA on vital fixed platelets. The expression of these molecules was evaluated in 20 healthy volunteers. The assay offers for the first time the possibility of screening the expression of receptor proteins on platelet membranes, which are related to indicate either a functional lack in bleeding disorders or a prethrombotic state due to an enhanced functional potential in high risk patients.
Tschöpe
D
D
Diabetes-Forschungsinstitut, Universität Düsseldorf.
Schauseil
S
S
Roesen
P
P
Kaufmann
L
L
Gries
F A
FA
D000911:Antibodies, Monoclonal; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence; D010976:Platelet Count; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D012016:Reference Values
D000911:Antibodies, Monoclonal; D010980:Platelet Membrane Glycoproteins
Darstellung von Thrombozytenmembranproteinen mit monoklonalen Antikörpern im durchflusszytometrischen Bio-Assay.