title: Immunochemical differentiation of glucose phosphate isomerase (GPI) allozymes of the mouse.
abstract: Polyclonal xenoantisera against mouse GPI-1B and GPI-1C were produced in rabbits and analyzed for their ability to recognize allozyme-specific determinants. These studies showed a high degree of serological similarity among the three allozymes of mouse glucose phosphate isomerase (GPI). However, GPI-1B and GPI-1C could be differentiated from GPI-1A as well as GPI-1A and GPI-1B from GPI-1C using quantitative solid-phase immunobinding assays. In addition, polyclonal and monoclonal alloantibodies specific for GPI-1C were produced in BALB/c (Gpi-1a/Gpi-1a) mice. As indicated by immunoblotting data, the allozyme specificity of rabbit antisera and monoclonal alloantibodies against GPI-1C is dependent on the native structure of that allozyme.
authors.affiliation: Zentralinstitut für Genetik und Kulturpflanzenforschung der AdW der DDR, Gatersleben, German Democratic Republic.
authors.forename: U
authors.initials: U
authors.lastname: Conrad
authors.forename: G
authors.initials: G
authors.lastname: Walter
authors.forename: D
authors.initials: D
authors.lastname: Helbing
authors.forename: R
authors.initials: R
authors.lastname: Manteuffel
authors.forename: J
authors.initials: J
authors.lastname: Schöneich
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis / Q000235:genetics*; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits
chemical_list: D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase