A component of Mycobacterium leprae as immunomodulating agent for immune deficient cells of leprosy patients.
The delipidified component of the insoluble portion which presumably is the cell wall of Mycobacterium leprae (DCW) was able to induce lymphocyte proliferation in the leucocyte culture from the peripheral blood of lepromatous leprosy patients. Normally these cells show no lymphocyte proliferation in response to M. leprae or their sonicated extract. The delipidified component (DCW) appears to be proteinaceous and able to induce antibodies in rabbit. The DCW has affinity to the sera from lepromatous leprosy patients but not sera from normal healthy individuals or tuberculoid leprosy patients. The ability to induce lymphocyte proliferation is blocked by agglutination of DCW with patient sera, heat treatment of DCW or protease treatment of the component. Along with lymphocyte proliferation, DCW also induces ability in the macrophages to render phagocytosed M. leprae non viable. Thus it is proposed that DCW of M. leprae could be a potent immunomodulator for immunedeficient cells of leprosy patients. The efficacy of DCW as a probable immunoprotector for M. leprae infection in mice has already been demonstrated earlier.
Robinson
P
P
Foundation for Medical Research, Bombay, India.
Mahadevan
P R
PR
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D001428:Bacterial Vaccines / Q000302:isolation & purification; D002473:Cell Wall / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007918:Leprosy / Q000276:immunology* / Q000517:prevention & control; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D009166:Mycobacterium leprae / Q000276:immunology*
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; C063154:delipified cell component, M leprae