Trichobilharzia ocellata: interference with endocrine control of female reproduction of Lymnaea stagnalis.
Calfluxin (CaFl), one of the gonadotropic hormones of Lymnaea stagnalis, stimulates the influx of Ca2+ into the mitochondria of the cells of the albumen gland, one of the accessory sex organs of the snail. This effect is suppressed in glands of noninfected snails by an agent (schistosomin) present in the hemolymph of snails infected by Trichobilharzia ocellata as shown in in vitro experiments. The agent is present from 6 weeks postinfection onward. Ca2+ deposits in the mitochondria were demonstrated with the ultracytochemical antimonate precipitation technique. The percentage of Ca2+-positive mitochondria was taken as a measure for the effects of CaFl. This percentage appeared to be greatly reduced when glands were incubated in serum of infected snails (Sinf). The data showed that Ringer incubations can serve as controls for experiments with serum: no differences were found between Ringer incubations and incubations in either fresh or frozen serum of noninfected snails. Schistosomin was not affected by freezing, which enables cold storage of Sinf. The dose-response relationship of schistosomin shows that at a 1:2 dilution of Sinf with Ringer the response to CaFl was reduced more than 50%. Schistosomin is heat-stable and Pronase-labile, which indicates that it has a peptide nature. Probably schistosomin(s) is responsible for the reduction/cessation of fecundity in trematode-infected snails.
de Jong-Brink
M
M
Department of Biology, Vrije Universiteit, Amsterdam, The Netherlands.
Elsaadany
M M
MM
Boer
H H
HH
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005298:Fertility; D006458:Hemolymph / Q000032:analysis* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D007447:Invertebrate Hormones / Q000037:antagonists & inhibitors; D008195:Lymnaea / Q000469:parasitology* / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000032:analysis / Q000648:ultrastructure; D009479:Neuropeptides / Q000037:antagonists & inhibitors*; D010455:Peptides / Q000494:pharmacology*; D012551:Schistosomatidae / Q000502:physiology* / Q000648:ultrastructure
D036341:Intercellular Signaling Peptides and Proteins; D007447:Invertebrate Hormones; D009479:Neuropeptides; D010455:Peptides; C052144:calfluxin; C054640:schistosomin