4-Methoxyindole derivatives as nitrosable precursors of mutagens in Chinese cabbage.
Two indole compounds isolated from fresh Chinese cabbage were shown to be mutagen precursors that yielded direct-acting mutagens on treatment with nitrite. They were identified as 4-methoxyindole-3-acetonitrile and 4-methoxy-indole-3-aldehyde. When these compounds were treated with 50 mM nitrite at pH 3.0 for 1 h at 37 degrees C, 4-methoxyindole-3-acetonitrile induced 31,800 and 10,000 revertants of Salmonella typhimurium TA100 and TA98, respectively, per mg of mutagen precursor in the absence of S9 mix, and 4-methoxyindole-3-aldehyde induced 156,900 revertants of TA100 and 26,800 revertants of TA98 per mg in the absence of S9 mix.
Wakabayashi
K
K
National Cancer Center Research Institute, Tokyo, Japan.
Nagao
M
M
Tahira
T
T
Yamaizumi
Z
Z
Katayama
M
M
Marumo
S
S
Sugimura
T
T
D000097:Acetonitriles / Q000302:isolation & purification / Q000633:toxicity*; D000447:Aldehydes / Q000302:isolation & purification / Q000633:toxicity*; D001937:Brassica / Q000032:analysis*; D007211:Indoles / Q000302:isolation & purification / Q000633:toxicity*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009152:Mutagenicity Tests; D009153:Mutagens / Q000302:isolation & purification*; D012977:Sodium Nitrite; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D000447:Aldehydes; D007211:Indoles; D009153:Mutagens; C057065:4-methoxyindole-3-acetonitrile; C057064:4-methoxyindole-3-aldehyde; D012977:Sodium Nitrite