title: Oxygen limitation induces indirectly the synthesis of cytochrome P-450 mRNA in alkane-growing Candida maltosa.
abstract: Candida maltosa cells grown on hexadecane under oxygen limitation have an up to 6-fold higher cytochrome P-450 content in comparison to cells cultivated at oxygen saturation. We show by mRNA quantification using an in vitro translation system and subsequent specific immunoprecipitation that the cytochrome P-450 induction occurs mainly on the transcriptional level. The signal for induction may be the enhanced intracellular hexadecane concentration owing to a reduced hydroxylation capacity of the cytochrome P-450 at oxygen limitation.
authors.affiliation: Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch.
authors.forename: B
authors.initials: B
authors.lastname: Wiedmann
authors.forename: M
authors.initials: M
authors.lastname: Wiedmann
authors.forename: S
authors.initials: S
authors.lastname: Mauersberger
authors.forename: W H
authors.initials: WH
authors.lastname: Schunck
authors.forename: H G
authors.initials: HG
authors.lastname: Müller
mesh_terms: D000332:Aerobiosis; D000473:Alkanes / Q000378:metabolism*; D000693:Anaerobiosis; D002175:Candida / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D007700:Kinetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D000473:Alkanes; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System