[Microphotometric system of analysis with automatic scanning and immunodetection: application to tissue sections (breast, endometrium, uterine cervix, ovary].
In the present study the immunodetection of various markers in a large series of tissue samples have been analysed through the computerized system of image analysis referred to as SAMBA 200. The immunodetection of estrogen and progesterone receptors, Laminin and type IV collagen, Ki 67, keratin, papilloma virus have been performed in frozen sections or in imprints and fine needle aspirates from tumorous and non tumorous disorders of human breast, endometrium, cervix and ovaries. Staining procedures varied with the type of the marker studied (PAP, ABC-P, ABC-GO, APAAP). The results obtained by the multiparametric SAMBA analysis were accurate, reproducible and reliable. Therefore, the SAMBA analysis providing a standardized and automatic analysis of immunodetection performed in tissue sections can be routinely used in pathology laboratories particularly for the prognostic evaluation and for the prediction of hormonotherapy and chemotherapy response of patients with solid malignant disorders.
Charpin
C
C
Laboratoire d'Anatomie Pathologique et de Neuropathologie, Secteur Nord, Faculté de Médecine, Marseille.
Martin
P M
PM
Andrac
L
L
Devictor
B
B
Vacheret
H
H
Lavaut
M N
MN
Jacquemier
J
J
Toga
M
M
D001943:Breast Neoplasms / Q000032:analysis; D003094:Collagen / Q000032:analysis; D004717:Endometrium / Q000032:analysis; D005260:Female; D005833:Genital Neoplasms, Female / Q000032:analysis*; D005836:Genitalia, Female / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000032:analysis; D010051:Ovarian Neoplasms / Q000032:analysis; D010784:Photomicrography; D011506:Proteins / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000032:analysis; D014594:Uterine Neoplasms / Q000032:analysis
D007797:Laminin; D011506:Proteins; D011980:Receptors, Progesterone; D003094:Collagen
Système d'analyse microphotométrique à balayage automatique (SAMBA) et immunodétection: applications sur coupes de tissu (sein, endomètre, col utérin, ovaire).