Cocaine produces fine structural nuclear alterations in cultured neuroglioblastoma cells.
NG 108-15 neuroglioblastoma cells were grown in culture medium containing either 10(-3), 10(-6) or 10(-9) M cocaine for 1-3 days. Some cultures were assessed for viability while others were processed for electron microscopy. Following 1-3 days of cocaine, no cytoplasmic alterations were observed compared to control; however, numerous dense bodies were present in some cells cultured with the highest doses. After 2 days of treatment with 10(-3) and 10(-6) M cocaine, nuclear invaginations were found filled with vesicles, and the nuclear membrane surrounding the vesicles was disrupted. Following 3 days of treatment with 10(-3) and 10(-6) M cocaine, patches of vesicles and tubules were also seen in the nucleus, but without surrounding membranes. The vesicles ranged in size from 0.05 to 0.8 micron. Cell counts revealed a significant slowing in the rate of cell division after two days of exposure to 10(-3) M cocaine. All concentrations of cocaine caused a significant decrease in cell viability by the third day of treatment. These results suggest that cocaine may interfere with cell replication and also may have a neurotoxic effect.
Johnson
J E
JE
Neuropharmacology Laboratory, National Institute on Drug Abuse, Baltimore, MD 21224.
Weissman
A D
AD
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D003042:Cocaine / Q000494:pharmacology*; D005910:Glioma; D008854:Microscopy, Electron; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure
D003042:Cocaine