title: Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response.
abstract: We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both stationary and exponential phase cells. These and other observations indicate that the mechanisms of repair of alkylation damage and mutagenesis differ markedly between S. cerevisiae and Escherichia coli.
authors.affiliation: Department of Radiation Biology and Biophysics, University of Rochester School of Medicine, NY 14642.
authors.forename: R
authors.initials: R
authors.lastname: Polakowska
authors.forename: G
authors.initials: G
authors.lastname: Perozzi
authors.forename: L
authors.initials: L
authors.lastname: Prakash
mesh_terms: D000222:Adaptation, Physiological; D000478:Alkylation; D002453:Cell Cycle; D003513:Cycloheximide / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects; D005020:Ethyl Methanesulfonate; D005800:Genes, Fungal; D008769:Methylnitronitrosoguanidine; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics*
chemical_list: D008769:Methylnitronitrosoguanidine; D003513:Cycloheximide; D005020:Ethyl Methanesulfonate