Structure of tumour necrosis factor by X-ray solution scattering and preliminary studies by single crystal X-ray diffraction.
The structure of tumour necrosis factor has been investigated by X-ray small-angle scattering and X-ray diffraction using synchrotron radiation. The overall radius of gyration is 25.5 A. A plausible model accounting for the scattering curves consists of an elongated trimer with an axial ratio of 3 to 4 and a maximal chord with a lower limit of 80 A. Tumour necrosis factor has been crystallized in a trigonal space group. Our results are in favour of a single trimer in the asymmetric unit. The diffraction extends to 3.5 A.
Lewit-Bentley
A
A
LURE, Université Paris-Sud, Orsay, France.
Fourme
R
R
Kahn
R
R
Prangé
T
T
Vachette
P
P
Tavernier
J
J
Hauquier
G
G
Niers
W
W
D006801:Humans; D008954:Models, Biological; D014409:Tumor Necrosis Factor-alpha; D014961:X-Ray Diffraction
D014409:Tumor Necrosis Factor-alpha