Separation of the pore-forming and cytotoxic activities from natural killer cell cytotoxic factor.
The influence of Ca2+ on the cytotoxic activity of natural killer cell cytotoxic factor (NKCF) was analyzed. When the natural killer susceptible cell line K562 was exposed to NKCF in the presence of 5 mM Ca2+, two peaks of cell damage were found. The first peak was observed after 30-40 min of incubation as a result of pore formation on the surface of target cells. The second was a peak of cytolytic activity which appeared after 24 h of incubation. Upon dilution of the NKCF preparation, only the first peak was observed. Therefore, NKCF produced by large granular lymphocytes in response to K562 consists of different proteins and represents pore-forming and cytolytic activities.
Sashchenko
L P
LP
Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Gnuchev
N V
NV
Kirillova
M A
MA
Lukjanova
T I
TI
Rebizova
T V
TV
Revazova
E S
ES
Lukanidin
E M
EM
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D055646:Killer Factors, Yeast; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D011506:Proteins / Q000276:immunology*
D055646:Killer Factors, Yeast; D011506:Proteins; D002118:Calcium