[Nickel chloride and alloxan. I. Determination of glucose, insulin and superoxide dismutase in blood and pancreas of rats].
Copper-zinc superoxide-dismutase (SOD-E.C. 1.15.1.1.) is present in high concentration in the beta-cells of pancreatic islets, and its specific activity correlates with maintenance of beta-cell function. In this paper the authors studied the effect of nickel chloride (s.c.) on alloxan toxicity. It was found that alloxan (100 mg x kg-1) inhibited insulin release of rats islets and thus, induced hyperglycemic response. The activity of erythrocytes and pancreatic SOD enzymes was partially inhibited upon alloxan treatment. It was found that nickel chloride (s.c. 10 mg x kg-1) produced stimulation of insulin release in rats treated by subcutaneous (s.c.) alloxan injection. The potential of NiCl2 to prevent alloxan induced diabetes was shown by the observed SOD specific activity increase in rats. In conclusion, our experiments show that nickel chloride prevented alloxan induced toxicity in rats.
Novelli
E L
EL
Departamento de Bioquímica, Instituto Básico de Biologia Médica e Agrícola, São Paulo, Brasil.
Rodrigues
N L
NL
Ribas Ozonas
B
B
D000496:Alloxan / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced* / Q000517:prevention & control; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009532:Nickel / Q000494:pharmacology*; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D013268:Stimulation, Chemical; D013482:Superoxide Dismutase / Q000378:metabolism*
D001786:Blood Glucose; D007328:Insulin; C022838:nickel chloride; D000496:Alloxan; D009532:Nickel; D013482:Superoxide Dismutase
Cloreto de níquel e aloxana. I. Determinação de glicose, insulina e superóxido-dismutase em sangue e pâncreas de ratos.