In vivo sister chromatid exchange and micronucleus induction studies with 1,3-butadiene in B6C3F1 mice and Sprague-Dawley rats.
Male B6C3F1 mice and Sprague-Dawley rats were exposed for 2 days, 6 h/day to 1,3-butadiene (BD) by inhalation (nose only) and their bone marrow cells were evaluated for the induction of micronuclei (MN) and sister chromatid exchanges (SCEs). A significant dose-dependent increase in MN induction was observed in mice. At 100 p.p.m., the frequency of micronucleated polychromatic erythrocytes was 6-fold above control with a maximal induction of 38-fold at 10,000 p.p.m. A significant increase in SCEs was also observed in mouse bone marrow cells starting at 100 p.p.m. with a 4-fold increase over the control evident at 10,000 p.p.m. The highest tested no observed effect level for both endpoints was 50 p.p.m. In contrast, rat bone marrow cells did not exhibit significant increases in micronucleated polychromatic erythrocytes or SCEs. These results indicate that BD is genotoxic in the bone marrow of the mouse but not the rat. This paralleled the chronic bioassays which showed mice to be more susceptible than rats to BD carcinogenicity.
Cunningham
M J
MJ
Haskell Laboratory for Toxicology and Industrial Medicine, E.I.Du Pont de Nemours and Co., Newark, DE 19714.
Choy
W N
WN
Arce
G T
GT
Rickard
L B
LB
Vlachos
D A
DA
Kinney
L A
LA
Sarrif
A M
AM
D000280:Administration, Inhalation; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D002070:Butadienes / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013045:Species Specificity
D002070:Butadienes; D009153:Mutagens; C031763:1,3-butadiene