title: Stimulus-dependent myristoylation of a major substrate for protein kinase C.
abstract: Bacterial lipopolysaccharide (LPS), the major surface component of gram-negative bacteria, exerts a profound effect on the immune system by enhancing the release of proteins and arachidonic acid metabolites from macrophages (for review see ref. 1). The molecular mechanism(s) by which LPS induces these various secretory responses is unknown. We previously reported that LPS promotes the myristoylation of several macrophage proteins including one with a relative molecular mass (Mr) of 68K2. We have now found that by several criteria the 68K myristoylated protein is similar or identical to the 80/87K protein, a major specific substrate for protein kinase C (PKC) found in brain and fibroblasts (for review see refs 7,8). We have also found that the myristoylated PKC substrate is quantitatively associated with the membrane fraction. Myristoylation of the PKC substrate may target it to the membrane and constitute a transduction pathway for stimulus-response coupling.
authors.affiliation: Rockefeller University, New York, New York 10021.
authors.forename: A A
authors.initials: AA
authors.lastname: Aderem
authors.forename: K A
authors.initials: KA
authors.lastname: Albert
authors.forename: M M
authors.initials: MM
authors.lastname: Keum
authors.forename: J K
authors.initials: JK
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Greengard
authors.forename: Z A
authors.initials: ZA
authors.lastname: Cohn
mesh_terms: D000818:Animals; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D009227:Myristic Acids; D011506:Proteins; D019814:Myristic Acid; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate