Scanning microfluorometric measurement of immunofluorescently labelled microtubules in cultured cells. Dependence of microtubule content on cell density.
A method for evaluation of microtubule content in cultured cells has been developed. The method is based on scanning microfluorometric measurement of immunofluorescently labelled microtubules. The method has been applied to the comparison of microtubule content in epithelial XTH-2 cells grown in culture at various cell densities. The results have shown that the microtubule content in the cells is not dependent on their proliferative state rather than it depends on cellular contacts.
Kajstura
J
J
Arbeitsgruppe Kinematische Zellforschung, Johann-Wolfgang-Goethe Universität, Frankfurt am Main, Federal Republic of Germany.
Bereiter-Hahn
J
J
D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D004727:Endothelium / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000648:ultrastructure*; D009206:Myocardium / Q000648:ultrastructure; D014981:Xenopus