title: Stimulation of mono (ADP-ribosyl)ation by reduced extracellular calcium levels in human fibroblasts.
abstract: Lowering extracellular calcium in cultures of human diploid fibroblast-like cells caused a rapid depletion of NAD pools. This loss of NAD was reversed by restoring extracellular Ca2+ and was inhibited by 3-aminobenzamide, an inhibitor of ADP-ribosyl transfer reactions. The concentrations of 3-aminobenzamide needed to inhibit the loss of NAD were consistent with those required to inhibit cellular mono(ADP-ribosyl) rather than poly(ADP-ribosyl) reactions. Calcium depletion did not inhibit the biosynthesis of NAD. These results suggest that mono(ADP-ribosyl)ation is involved in the regulation of cellular Ca2+ levels.
authors.affiliation: Samuel Roberts Noble Foundation, Inc., Ardmore, Oklahoma 73402.
authors.forename: M R
authors.initials: MR
authors.lastname: Duncan
authors.forename: P R
authors.initials: PR
authors.lastname: Rankin
authors.forename: R L
authors.initials: RL
authors.lastname: King
authors.forename: M K
authors.initials: MK
authors.lastname: Jacobson
authors.forename: R T
authors.initials: RT
authors.lastname: Dell'Orco
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005110:Extracellular Space / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D008297:Male; D009243:NAD / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013268:Stimulation, Chemical
chemical_list: D009243:NAD; D000246:Adenosine Diphosphate Ribose; D002118:Calcium