Stimulation of mono (ADP-ribosyl)ation by reduced extracellular calcium levels in human fibroblasts.
Lowering extracellular calcium in cultures of human diploid fibroblast-like cells caused a rapid depletion of NAD pools. This loss of NAD was reversed by restoring extracellular Ca2+ and was inhibited by 3-aminobenzamide, an inhibitor of ADP-ribosyl transfer reactions. The concentrations of 3-aminobenzamide needed to inhibit the loss of NAD were consistent with those required to inhibit cellular mono(ADP-ribosyl) rather than poly(ADP-ribosyl) reactions. Calcium depletion did not inhibit the biosynthesis of NAD. These results suggest that mono(ADP-ribosyl)ation is involved in the regulation of cellular Ca2+ levels.
Duncan
M R
MR
Samuel Roberts Noble Foundation, Inc., Ardmore, Oklahoma 73402.
Rankin
P R
PR
King
R L
RL
Jacobson
M K
MK
Dell'Orco
R T
RT
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005110:Extracellular Space / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D008297:Male; D009243:NAD / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013268:Stimulation, Chemical
D009243:NAD; D000246:Adenosine Diphosphate Ribose; D002118:Calcium