Parameters affecting the maximum cell concentration of Tetrahymena.
In cultures with efficient aeration a maximum cell concentration (MCC) of 6 X 10(5) cells/ml (defined medium) and 5.5 X 10(6) cells/ml (broth) can be reached. By culturing within Millicells with excess supply of medium and efficient removal of waste products a physical limit for MCC of about 13 X 10(6) cells/ml is reached.
Hellung-Larsen
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Department of Biochemistry B, University of Copenhagen, Denmark.
D000818:Animals; D002241:Carbohydrates; D002452:Cell Count; D002455:Cell Division; D003470:Culture Media; D005285:Fermentation; D013768:Tetrahymena / Q000166:cytology / Q000254:growth & development*
D002241:Carbohydrates; D003470:Culture Media