title: Assessment of aneuploidy in the human female by using cytogenetics of IVF failures.
abstract: The karyotype was determined for 201 unfertilized human oocytes recovered from 87 women participating in an in vitro fertilization program. Their mean age was 30.6 years (range 22-40 years). Thirteen oocytes did not exhibit the first polar body and were found to be in the first-metaphase stage. The remaining 188 eggs were assessed as mature and classified into two groups according to the supposed origin of fertilization failure. The overall incidence of aneuploidy was 18.6%. This rate was higher in the group of unexplained fertilization failure (22.5%) than in the group of fertilization failure due to sperm deficiency (10.2%). This may reflect a relationship between the genomic constitution of the oocyte and its fertilizability. On the other hand, no increase of aneuploidy was observed with maternal aging.
authors.affiliation: Cytogenetic and Reproductive Biology Laboratory, Grenoble University Medical School, La Tronche, France.
authors.forename: F
authors.initials: F
authors.lastname: Pellestor
authors.forename: B
authors.initials: B
authors.lastname: Sèle
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002871:Chromosome Banding; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007621:Karyotyping; D008423:Maternal Age; D009865:Oocytes / Q000648:ultrastructure*