Induction of S 100 protein by 5-bromo-2'-deoxyuridine in human neuroblastoma cell lines.
The simultaneous effect of 5-bromo-2'-deoxyuridine (BrdUrd) on cell growth, morphological changes, and cellular contents of S 100 (S 100ao and S 100b) protein and neuron specific enolase was investigated in human neuroblastoma cells in culture. Among four cell lines (NCG, SK-N-DZ, GOTO, NB-1), GOTO was the most affected. With 5 micrograms/ml BrdUrd, the growth of this cell line was significantly inhibited to 14.5% of the control on day 6, in association with morphological changes into flat-type cells and an increase of S 100 protein. S 100ao protein was increased from 37 to 211,000 pg/mg protein (5,600-fold) and S 100b protein from less than 25 to 623 pg/mg protein. The induction of S 100 protein was also seen in SK-N-DZ but not in NCG and NB-1. In GOTO the induction of S 100 protein occurred in a dose- and time-dependent manner by the treatment with BrdUrd. On the other hand, after exposure to BrdUrd, neuron specific enolase decreased in NB-1 and SK-N-DZ and increased in GOTO. These results suggest that although heterogeneous certain neuroblastoma cell lines could be differentiated into S 100 protein-positive cells that may represent glial or Schwann cells and that the effect of BrdUrd is exerted bidirectionally in neuroblastoma differentiation.
Tsunamoto
K
K
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
Todo
S
S
Imashuku
S
S
Kato
K
K
D001973:Bromodeoxyuridine / Q000378:metabolism / Q000494:pharmacology*; D002454:Cell Differentiation; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D009418:S100 Proteins / Q000096:biosynthesis*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism
D009418:S100 Proteins; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine