Assay of 2-hydroxyputrescine in various regions of rat brain by gas chromatography-mass spectrometry.
2-Hydroxyputrescine in seven regions of single rat brains was measured with a sensitive, specific assay by gas chromatography-mass spectrometry. The regions were the cerebral cortex, cerebellum, medulla oblongata, hypothalamus, striatum, hippocampus, and midbrain. The level of 2-hydroxyputrescine was very high in the cerebral cortex and cerebellum, high in the medulla oblongata, hypothalamus, and hippocampus, and low in the striatum and midbrain. The level of 2-hydroxyputrescine in the cerebellum was significantly higher than in the striatum and midbrain.
Noto
T
T
Department of Psychiatry, Kyoto Prefectural University of Medicine, Japan.
Hasegawa
T
T
Hashimoto
H
H
Nakajima
T
T
D000818:Animals; D001923:Brain Chemistry; D002531:Cerebellum / Q000032:analysis; D002540:Cerebral Cortex / Q000032:analysis; D003342:Corpus Striatum / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006624:Hippocampus / Q000032:analysis; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D008526:Medulla Oblongata / Q000032:analysis; D008636:Mesencephalon / Q000032:analysis; D011700:Putrescine / Q000031:analogs & derivatives* / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
C002952:2-hydroxyputrescine; D011700:Putrescine