title: Comparative study of the mucin-type sugar chains of human chorionic gonadotropin present in the urine of patients with trophoblastic diseases and healthy pregnant women.
abstract: Human chorionic gonadotropins (hCGs) highly purified from the urine of patients with trophoblastic diseases and of healthy pregnant women contain approximately four mucin-type sugar chains in one molecule. The structures of these sugar chains were studied comparatively by using a new sensitive method to obtain mucin-type sugar chains quantitatively as radioactive oligosaccharides from a small amount of glycoproteins. The mucin-type sugar chains of all hCGs include sialylated and nonsialylated Gal beta 1----3GalNAc and Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAc. In the case of normal hCG and hydatidiform mole hCG, oligosaccharides containing the tetrasaccharide core occupy approximately 10% of the total mucin-type sugar chains. The ratio of the tetrasaccharide containing oligosaccharides is increased prominently to approximately 60% in choriocarcinoma hCG. The proportion in invasive mole hCG was also increased, but less than the proportion of choriocarcinoma hCG.
authors.affiliation: Department of Biochemistry, University of Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Amano
authors.forename: R
authors.initials: R
authors.lastname: Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Mochizuki
authors.forename: A
authors.initials: A
authors.lastname: Kobata
mesh_terms: D000107:Acetylation; D002236:Carbohydrate Conformation; D002822:Choriocarcinoma / Q000652:urine*; D006063:Chorionic Gonadotropin / Q000652:urine*; D002854:Chromatography, Paper; D004590:Electrophoresis, Paper; D005260:Female; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D006828:Hydatidiform Mole / Q000652:urine*; D009077:Mucins / Q000032:analysis*; D009439:Neuraminidase / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis; D011247:Pregnancy / Q000652:urine*; D014594:Uterine Neoplasms / Q000652:urine*
chemical_list: D006063:Chorionic Gonadotropin; D009077:Mucins; D009844:Oligosaccharides; D006026:Glycoside Hydrolases; D009439:Neuraminidase