Biosynthesis of sialosyllactotetraosylceramide in human colorectal carcinoma cells.
A monosialoganglioside, IV3-NeuNAcLcOse4Cer, has recently been detected in colorectal carcinoma cells, small cell lung carcinoma cells, embryonal carcinoma cells and in human brain extracts. We report here the presence of a CMP-sialic: LcOse4Cer sialyl transferase activity in subcellular membrane fractions of the human colorectal carcinoma. SW1116, which recognizes the non-reducing terminal galactosyl moiety of lactotetraosylceramide. A convenient method for structural analysis of picomolar quantities of the radioactive enzymatic product(s) using bacterial endoglycoceramidase, sialidase and a viral sialidase is presented.
Jolif
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A
IRSC-CNRS, ER 277, Villejuif, France.
Liepkans
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V
D000951:Antigens, Neoplasm; D003110:Colonic Neoplasms / Q000378:metabolism*; D005915:Globosides / Q000096:biosynthesis*; D006028:Glycosphingolipids / Q000096:biosynthesis*; D006801:Humans; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism; D012004:Rectal Neoplasms / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism; D012799:Sialyltransferases / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D005915:Globosides; D006028:Glycosphingolipids; D012794:Sialic Acids; C037790:sialosylgloboside; D012799:Sialyltransferases; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid