title: 80% of muscarinic receptors expressed by the NB-OK 1 human neuroblastoma cell line show high affinity for pirenzepine and are comparable to rat hippocampus M1 receptors.
abstract: The NB-OK 1 human neuroblastoma cell line expressed muscarinic cholinergic receptors that could be labeled with N-[3H]methylscopolamine (a nonselective antagonist). 80% of these receptors showed high affinity for pirenzepine, i.e. belonged to the M 1 subtype found in neuronal tissues. Their binding properties were identical to those of rat hippocampus M 1 receptors, and differed from those of rat pancreas and heart muscarinic receptors. The remaining (20%) muscarinic receptors showed low affinity for pirenzepine and AF-DX 116, being therefore of an M2 beta (or B) subtype, and were similar to rat pancreatic receptors.
authors.affiliation: Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Waelbroeck
authors.forename: J
authors.initials: J
authors.lastname: Camus
authors.forename: M
authors.initials: M
authors.lastname: Tastenoy
authors.forename: J
authors.initials: J
authors.lastname: Christophe
mesh_terms: D000818:Animals; D002460:Cell Line; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009447:Neuroblastoma / Q000378:metabolism*; D010890:Pirenzepine / Q000378:metabolism*; D051381:Rats; D011976:Receptors, Muscarinic / Q000378:metabolism*
chemical_list: D011976:Receptors, Muscarinic; D010890:Pirenzepine