The complete sequence of a frog alpha-tubulin gene and its regulated expression in mouse L-cells.
A frog alpha-tubulin cDNA and a frog alpha-tubulin gene, closely related to the cDNA, were cloned and sequenced and the structure of the gene deduced. The gene was introduced into mouse L-cells in order to investigate the transcription and regulation of the gene. The gene was transcribed and there was processing of the transcripts. Furthermore, the gene displayed the correct autoregulatory feedback control.
Smith
D J
DJ
MRC Laboratory of Molecular Biology, Cambridge, U.K.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005796:Genes; D006706:Homeostasis; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D014158:Transcription, Genetic; D014162:Transfection; D014404:Tubulin / Q000235:genetics*; D014982:Xenopus laevis
D014404:Tubulin; D004247:DNA