title: Changes in fetal organ flow during intrauterine mechanical ventilation with or without oxygen.
abstract: To investigate whether the changes in circulation at birth are due to lung ventilation, changes in PaO2 or both we mechanically ventilated in utero the lungs of 10 fetal sheep (120-127 days of gestational age) five days after instrumentation under general anaesthesia. Electrocortical activity (ECoG), eye movements (EOG), electromyographic activity from diaphragm and posterior neck activity (EMG) and electrocardiogram (ECG) were recorded. Fetal catheters (artery and vein of the hindlimb, arteries of both forelimbs which in three occasions were advanced into the left ventricle, fetal trachea and amniotic cavity), and an endotracheal tube were placed. After recovery radioactive 15 mu microspheres (I125, Ce141, Sr85 and Sc46) were injected into the inferior vena cava or left ventricle during high voltage electrocortical activity before and after lung expansion with N2 and after expansion with O2 for two levels of PaO2. PaCO2 did not change. The percentage of spheres trapped in the lungs increased from 9.6% to 44% after expanding the lungs with N2 and to 90% when fetal PaO2 increased (P less than 0.001). Blood flow to different organs did not change during normoxic expansion but it decreased significantly to the brain (91 +/- 25 to 27 +/- 8 ml/min per 100g, [mean +/- SD]) placenta (160 +/- 57 to 54 +/- 33 ml/min/100g) and coronaries (239 +/- 91 to 117 +/- 60 ml/min per 100g) when PaO2 was increased. In conclusion fetal circulation responds to raised levels of PaO2 well before birth probably by a direct action of oxygen on the vessels.
authors.affiliation: Department of Pediatrics, University Hospital, Maastricht, The Netherlands.
authors.forename: C E
authors.initials: CE
authors.lastname: Blanco
authors.forename: C B
authors.initials: CB
authors.lastname: Martin
authors.forename: J
authors.initials: J
authors.lastname: Rankin
authors.forename: M
authors.initials: M
authors.lastname: Landauer
authors.forename: T
authors.initials: T
authors.lastname: Phernetton
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D001794:Blood Pressure; D001921:Brain / Q000098:blood supply; D003326:Coronary Circulation; D005314:Embryonic and Fetal Development; D005260:Female; D006340:Heart Rate, Fetal; D000860:Hypoxia / Q000503:physiopathology*; D010100:Oxygen / Q000502:physiology*; D011247:Pregnancy; D012039:Regional Blood Flow; D012121:Respiration, Artificial; D012756:Sheep; D014655:Vascular Resistance
chemical_list: D010100:Oxygen