title: Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells.
abstract: We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells.
authors.affiliation: Cetus Corporation, Emeryville, California 94608.
authors.forename: T R
authors.initials: TR
authors.lastname: Chiang
authors.forename: L
authors.initials: L
authors.lastname: McConlogue
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005784:Gene Amplification; D005796:Genes; D005822:Genetic Vectors; D009955:Ornithine Decarboxylase / Q000235:genetics*; D010053:Ovary; D010957:Plasmids; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D009955:Ornithine Decarboxylase