Purging breast cancer cells in preparation for autologous bone marrow transplantation.
Mixtures of the T-47D human breast cancer cell line and normal human bone marrow cells were used for studying a new approach for purging epithelial tumor cells for autologous bone marrow transplantation (BMT) in breast cancer. Breast cancer cell line T-47D cells were shown to bind soybean agglutinin (SBA) in a specific fashion that could be blocked by D-galactose. Tumor cells were effectively purged by both SBA agglutination and depletion of cells bound to magnetic beads (0.7-5.0 micron) covalently linked to SBA. A depletion of 3-4 orders of magnitude of tumor cells was consistently accomplished by combining one step of agglutination followed by one cycle of SBA-magnetic bead depletion. Neither procedure affects stem cell recovery. We suggest that effective purging of breast cancer cells can be accomplished using SBA for autologous BMT in patients with advanced breast cancer.
Morecki
S
S
Department of Bone Marrow Transplantation and Immunobiology Research, Hadassah University Hospital, Jerusalem, Israel.
Pavlotzky
F
F
Margel
S
S
Slavin
S
S
D000371:Agglutination; D014408:Biomarkers, Tumor / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D016026:Bone Marrow Transplantation; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008863:Microspheres; D009362:Neoplasm Metastasis / Q000473:pathology*; D011975:Receptors, Mitogen / Q000032:analysis; D013025:Glycine max / Q000378:metabolism; D013535:Suspensions
D014408:Biomarkers, Tumor; D011975:Receptors, Mitogen; D013535:Suspensions