A pyruvate kinase variant in different mouse transplanted tumors.
Mouse transplanted tumors, in contrast to normal tissues, contain a pyruvate kinase (PK) variant sensitive to the inhibitory action of L-cysteine and less sensitive to saturated fatty acids than the normal enzyme. In selected normal and tumor materials two fractions of PK were separated. Fraction A (20-30% (NH4)2SO4 saturation) dominated in normal liver, and fraction B (50-60% (NH4)2SO4 saturation) in skeletal muscles and Ehrlich ascites tumor. Only this fraction B from tumor material was sensitive to L-cysteine, and seems to contain a tumor-specific PK variant which might be considered as a marker of neoplastic transformation in a broad spectrum of mouse experimental tumors.
Marchut
E
E
Institute of Medical Biochemistry, Nicolaus Copernicus Academy of Medicine, Cracow, Poland.
Gumińska
M
M
Kedryna
T
T
Radzikowski
C
C
Kuśnierczyk
H
H
D000818:Animals; D014408:Biomarkers, Tumor; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D003545:Cysteine / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D014644:Genetic Variation; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000201:enzymology*; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
D014408:Biomarkers, Tumor; D005227:Fatty Acids; D011770:Pyruvate Kinase; D003545:Cysteine