Diacylglycerol acyltransferase in maturing safflower seeds: its influences on the fatty acid composition of triacylglycerol and on the rate of triacylglycerol synthesis.
Diacylglycerol acyltransferase in a particulate preparation from maturing safflower seeds showed no strict selectivity for acyl-CoA when acyl-CoA substrates were administered as mixtures. This suggested that the fatty acid composition of position 3 in safflower triacyl-sn-glycerol exclusively depends on the acyl-CoA composition in the cell. The specific activity of the acylation was approximately 3 nmol/min per mg protein of the preparation under the optimum assay conditions. This low activity and other data appeared to indicate that the diacylglycerol acyltransferase reaction may be the rate-limiting step in triacylglycerol synthesis in vivo.
Ichihara
K
K
Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.
Takahashi
T
T
Fujii
S
S
D000217:Acyltransferases / Q000378:metabolism*; D051048:Diacylglycerol O-Acyltransferase; D005227:Fatty Acids / Q000032:analysis; D012639:Seeds / Q000201:enzymology*; D013379:Substrate Specificity; D014280:Triglycerides / Q000096:biosynthesis*
D005227:Fatty Acids; D014280:Triglycerides; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase