Predominant association of adenovirus type 12 DNA with human chromosome 1 early in productive infection.
In human KB cells productively infected with human adenovirus type 12 (Ad12), viral DNA can recombine with cellular DNA. The functional significance of this covalent linkage is not understood. It is, however, conceivable that very early in the infection cycle integrated viral genes gain an advantage by being transcribed more effectively. In the present study, it has been shown that Ad12 DNA is associated predominantly with human chromosome number 1 and one chromosome in the CII group. This association has been detected as early as 2 hr after infection by in situ hybridization. Further work will be directed toward the analysis of viral transcription products within the first 2 hr of the infection cycle.
Rosahl
T
T
Institute of Genetics, University of Cologne, Germany.
Doerfler
W
W
D000260:Adenoviruses, Human / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D014779:Virus Replication
D004279:DNA, Viral