Detection of genes with potential of suppressing transforming activity of the v-Ki-ras oncogene.
We have tried to detect genes which are expressed in normal cells and have the potential of suppressing transforming activity of the Kirsten sarcoma virus oncogene, v-Ki-ras. The experiments involved isolation and characterization of flat revertants from v-Ki-ras-transformed NIH/3T3 cells following either chemical mutagenesis or transfection of a cDNA expression library constructed from total mRNA of normal human fibroblasts. By these procedures multiple genes could be detected which, when transcriptionally activated, suppress different spectra of transformation-associated properties in spite of persistent expression of the v-Ki-ras gene product.
Noda
M
M
Laboratory of Molecular Oncology, Riken Tsukuba Life Science Center, Ibaraki, Japan.
Ikawa
Y
Y
D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000235:genetics; D011905:Genes, ras; D006801:Humans; D013489:Suppression, Genetic; D014162:Transfection
D004247:DNA