Dose fractionation and hepatocyte clonogens: alpha/beta congruent to 1-2 Gy, and beta decreases with increasing delay before assay.
The sensitivity to X-ray dose fractionation was assessed for hepatocytes forming colonies in fat pads in mice. When the cells were assayed 1 day after the last irradiation the alpha/beta ratio was 1.0-1.6 depending on the method of analysis. The alpha/beta ratio describing the shape of the single-dose survival curve was much higher, and it did not predict the response to fractionation. When the assay was delayed for 10 months after the fractionated irradiation, the alpha/beta ratio was 1.9-2.1, and the beta component showed the greatest change with time. It is concluded that hepatocytes respond to dose fractionation in a manner expected of a late-responding tissue, even when the cells are assayed as early as 1 day after the last dose.
Fisher
D R
DR
Radiobiology Department, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, United Kingdom.
Hendry
J H
JH
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D008099:Liver / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D051379:Mice; D011829:Radiation Dosage; D013234:Stem Cells / Q000528:radiation effects*