Investigation of a Providencia rettgeri strain that has enterobacterial common antigen in the immunogenic state.
Antigenic material obtained by phenol-water extraction from Providencia rettgeri strains, Escherichia coli O:14 strains, and mutants of the E. coli O:14 strain were examined by the passive (indirect) hemagglutination technique, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and by immune blotting (lipopolysaccharide (LPS) blotting). Providencia rettgeri 965, like E. coli O:14, was demonstrated to have an enterobacterial common antigen (ECA) in the immunogenic form but, unlike E. coli O:14, it possessed characteristics of a smooth strain. Two populations of molecules were observed to occur in P. rettgeri 965 phenol-water extracts: one consisting of LPS identifiable with specific O antisera and the other of ECA molecules identifiable with E. coli O:14 antiserum or with a monoclonal antibody against ECA.
Quigley
R C
RC
Department of Microbiology, Faculty of Medicine, University of Toronto, Ont., Canada.
Penner
J L
JL
D000942:Antigens, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000276:immunology*; D006384:Hemagglutination; D011511:Proteus / Q000276:immunology*; D011532:Providencia / Q000276:immunology*; D013045:Species Specificity
D000942:Antigens, Bacterial; C017286:enterobacterial common antigen