Suppressive effect of ipriflavone on bone depletion in the experimental diabetic rat: dose response of ipriflavone.
The dose dependent effect of ipriflavone (7-isopropoxy-isoflavone) on the femoral bone in streptozotocin-induced diabetic rats was studied by microdensitometric analysis. Diabetic rats showed severe hyperglycemia, glucosuria, hypoinsulinemia, associated with increased urinary calcium and hydroxyproline. Microdensitometric analysis revealed decreases in femoral length, bone width, and bone density. The dietary administration of ipriflavone (about 270 mg/kg/day) to the diabetic rats for 6 weeks prevented reduction of the cortical thickness index in the diaphysis and depletion of bone density in the distal metaphysis, and also reduced the inner diameter of the diaphysis; diabetic state was not improved. A simple correlation and linear regression analysis revealed that ipriflavone also significantly reduced the inner diameter in the diaphysis at a dose of 90 mg/kg/day, but not at one of 25 mg/kg/day. These results suggest that ipriflavone suppresses the depletion of the femoral bone through inhibition of bone resorption in a dose dependent fashion; its minimum effective dose is 90 mg/kg/day in experimental diabetes.
Shino
A
A
Biology Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
Tsukuda
R
R
Odaka
H
H
Kitazaki
T
T
Tsuda
M
M
Matsuo
T
T
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001862:Bone Resorption / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology* / Q000652:urine; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D005269:Femur / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011921:Rats, Inbred WKY
D005419:Flavonoids; D007529:Isoflavones; C018986:ipriflavone