title: Elevated DNA polymerase beta activity in a cis-diamminedichloroplatinum(II) resistant P388 murine leukemia cell line.
abstract: The activity of DNA polymerase beta, which is an enzyme involved in repair of DNA damage, was assessed in P388 murine leukemia cell lines sensitive and resistant to cis-diamminedichloroplatinum(II) (cis-Pt). The resistant line was selected with cis-Pt and showed cross-resistance to a number of alkylating agents, but not to an anthracycline. The DNA polymerase beta activity was found to be elevated 5-fold in the resistant line based upon activity per mg cell protein and elevated 4-fold based upon activity per 10(7) cells. The characterization of elevated activity of an enzyme active in DNA repair in a cell line resistant to DNA damaging agents describes a possible mechanism of resistance in addition to those previously found.
authors.affiliation: Department of Chemotherapy, Warner-Lambert/Parke-Davis Pharmaceutical Research, Ann Arbor, MI 48105.
authors.forename: A J
authors.initials: AJ
authors.lastname: Kraker
authors.forename: C W
authors.initials: CW
authors.lastname: Moore
mesh_terms: D000818:Animals; D002945:Cisplatin / Q000494:pharmacology*; D004256:DNA Polymerase I / Q000032:analysis*; D004260:DNA Repair; D004351:Drug Resistance; D007941:Leukemia P388 / Q000201:enzymology*; D007942:Leukemia, Experimental / Q000201:enzymology*; D051379:Mice; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D004256:DNA Polymerase I; D002945:Cisplatin