Genotoxicity of two fecal steroids in murine colonic epithelium assessed by the sister chromatid exchange technique.
Two components of human feces are known to induce nuclear anomalies in mice when applied intrarectally, but to be nonmutagenic in Salmonella. We have tested these two compounds for their ability to induce sister chromatid exchanges in the colonic epithelium of mice, the same tissue in which they induce nuclear anomalies when administered by the same route. One, 4-cholesten-3-one, induced sister chromatid exchanges whereas the other, 5-alpha-cholestan-3-one did not, even at the maximum feasible dose. The results suggest that 4-cholesten-3-one is more likely to be a significant factor in human colon cancer than the 5-alpha analog.
Kaul
H K
HK
Department of Biology, York University, Ontario, Canada.
Couch
D B
DB
Gingerich
J D
JD
Bruce
W R
WR
Heddle
J A
JA
D000818:Animals; D002776:Cholestanes / Q000633:toxicity*; D002782:Cholestenes / Q000633:toxicity*; D002783:Cholestenones / Q000633:toxicity*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005243:Feces / Q000032:analysis; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009152:Mutagenicity Tests; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D002776:Cholestanes; D002782:Cholestenes; D002783:Cholestenones; C000475:cholest-4-en-3-one; C007123:coprostanone