[Submerged-phase gel electrophoresis in the analysis of plasmids for epidemiologic purposes].
The validity of the submarine agarose gel electrophoresis as routine method for plasmidic epidemiology was considered. Using standard plasmids, the efficiency of a performed protocol conditions (5 V/cm for 3 hours, 0.8% agarose) in screening plasmids of different size was studied. The results provide sharp profile resolution and an accurate estimate of molecular weights in the range from 1.2 to 112 megadaltons.
Caldini
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G
Dipartimento di Igiene Università, Perugia.
Cenci
G
G
Morozzi
G
G
D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005069:Evaluation Studies as Topic; D008970:Molecular Weight; D010957:Plasmids; D012015:Reference Standards
Gel elettroforesi in fase sommersa nell'analisi plasmidica a fini epidemiologici.