Regen: program for designing gene assembly.
One of the main problems in constructing synthetic genes is the incorrect hybridisation between the oligonucleotides. The problem is resolved if the sequence uniquely defines the position of the oligonucleotide in the assembled gene. This can be accomplished through the wise partition of dsDNA sequence in the fragments. We describe a program for use in designing such gene assembly. For a given DNA sequence and the approximate location of oligonucleotide boundary it generates all sets of protruding ends that share the smallest homology.
Jerala
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Department of Biochemistry, Jozef Stefan Institute, Ljubljana, Yugoslavia.
Turk
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D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D015891:Cystatins; D005813:Genes, Synthetic; D008838:Microcomputers; D011506:Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012984:Software / Q000379:methods*
D015891:Cystatins; D011506:Proteins; C100158:cystatin, egg-white