Linearization of two ligand-one binding site scatchard plot and the "IC50" competitive inhibition plot: application to the simplified graphical determination of equilibrium constants.
A simple procedure for linearizing the curved, two ligand-one binding site Scatchard plot resulting from the presence of a constant concentration of competitive inhibitor is proposed; the same procedure may also be applied to the analysis of data derived from the "IC50" competitive inhibition experimental design. Furthermore, a useful generalization of the Cheng-Prusoff correction is presented.
Gray
H E
HE
Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610.
Luttge
W G
WG
D001665:Binding Sites; D001667:Binding, Competitive; D008024:Ligands / Q000378:metabolism*; D008433:Mathematics
D008024:Ligands