Phosphatidylserine increases in vivo the synaptosomal uptake of exogenous GABA in rats.
A sonicated liposome suspension of gamma-aminobutyric acid (GABA) and phosphatidylserine (liposome-entrapped GABA), intraperitoneally administered in rats, inhibited EEG epileptic activity induced by penicillin, whereas GABA did not. A significant increase (20.4%) in brain radioactivity accumulation occurred at 5 min after i.p. administration of [14C]GABA associated with phosphatidylserine in comparison with the administration of [14C]GABA; such an increase persisted after 20 min. However, the accumulation of radioactivity into brain synaptosomes demonstrated a 24.1% increase at 5 min and subsequently showed a 43.3% increase at 20 min after injection of liposome-entrapped GABA. The above findings suggest that phosphatidylserine stimulates exogenous GABA uptake into brain GABAergic nerve terminals.
Loeb
C
C
Institute of Neurology, University of Genova, Italy.
Marinari
U M
UM
Benassi
E
E
Besio
G
G
Cottalasso
D
D
Cupello
A
A
Maffini
M
M
Mainardi
P
P
Pronzato
M A
MA
Scotto
P A
PA
D000818:Animals; D001921:Brain / Q000378:metabolism; D008081:Liposomes / Q000008:administration & dosage; D008297:Male; D010718:Phosphatidylserines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000493:pharmacokinetics*
D008081:Liposomes; D010718:Phosphatidylserines; D005680:gamma-Aminobutyric Acid