The role of rat liver microsomal enzymes in the metabolism of the fungal metabolite fusarin C.
The metabolic activation of fusarin C by a rat liver microsomal monooxygenase resulted in the formation of a water-soluble mutagenic metabolite. However, fusarin C incubated in the presence of a microsomal preparation, but in the absence of an NADPH-generating system, led to the formation of fusarin PM1, a highly water-soluble compound which, like fusarin C, requires metabolic activation to be mutagenic. Enzyme studies using as substrates fusarins A and D, compounds structurally related to fusarin C, together with structural studies of fusarin PM1 indicated that fusarin PM1 was formed by the action of carboxylesterase which hydrolyses the C-20 methyl ester group to a free carboxylic acid.
Gelderblom
W C
WC
Research Institute for Nutritional Diseases, South African Medical Research Council, Tygerberg.
Thiel
P G
PG
van der Merwe
K J
KJ
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000502:physiology*; D004851:Epoxide Hydrolases / Q000502:physiology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology* / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D011090:Polyenes / Q000378:metabolism*; D051381:Rats
D009153:Mutagens; D011090:Polyenes; C041403:fusarin C; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D004851:Epoxide Hydrolases