[Immunocytological distribution of HP1 group protamines in human testes and ejaculated spermatozoa].
Specific antisera have been obtained against the purified human sperm protamine HP1b. The specificity of antibodies was assessed in dot-blot and western-blot assays. Antibodies showed high binding to protamine HP1 group. Nevertheless cross-reactivity between HP1 and the other protamines (HP2 - HP3 - HP4) could not be excluded. Immunohistochemical studies revealed that protamines HP1 were detected in nuclei of ejaculated sperm after chromatin decondensation. In testis, they appeared in nuclei of elongating spermatids.
Roux
C
C
Laboratoire d'Histologie-Embryologie, Faculté de Médecine Broussais Hôtel-Dieu, Paris.
Mathieson
J
J
Dadoune
J P
JP
D004542:Ejaculation; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D011479:Protamines / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*; D013737:Testis / Q000032:analysis*
D007106:Immune Sera; D011479:Protamines; C046533:protamine P1
Localisation immunocytologique des protamines du groupe HP1 dans le testicule humain et le spermatozoïde éjaculé.