Do DNA polymerases delta and alpha act coordinately as leading and lagging strand replicases?
The activity ratio of DNA polymerases delta and alpha in calf thymus was found to be invariably 1:1, irrespective of extraction procedure (8 types) and subcellular localization (cytoplasm, nucleus and microsomes). This was established by separation of the two forms by hydroxyapatite chromatography and by their response to specific inhibitors and monoclonal antibodies. This finding supports the dimeric DNA polymerase model [(1980) J. Biol. Chem. 255, 4290-4303], which proposes that DNA polymerases delta and alpha act coordinately as leading and lagging strand enzymes, respectively, at the replication fork.
Focher
F
F
Department of Pharmacology and Biochemistry, University of Zürich-Irchel, Switzerland.
Ferrari
E
E
Spadari
S
S
Hübscher
U
U
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002850:Chromatography, Gel; D004257:DNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D004258:DNA Polymerase III / Q000302:isolation & purification / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003848:Deoxyguanine Nucleotides / Q000378:metabolism; D008957:Models, Genetic; D013950:Thymus Gland / Q000201:enzymology
D000911:Antibodies, Monoclonal; D003848:Deoxyguanine Nucleotides; C040364:N(2)-(4-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate; D000255:Adenosine Triphosphate; C047394:4-n-butylanilino dATP; C023140:DNA replicase; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase