Synthetic pentapeptide from the B1 chain of laminin promotes B16F10 melanoma cell migration.
Laminin is a basement membrane-specific glycoprotein that promotes cell adhesion, proliferation, differentiation, and tumor cell migration. Synthetic peptides from the amino acid sequence deduced from a cDNA clone of the B1 chain of laminin were tested for their ability to promote the migration of B16F10 melanoma cells. A peptide, CDPGYIGSR, that is able to mediate epithelial cell attachment to laminin was found to promote migration, and the constituent pentapeptide YIGSR was also active but to a lesser degree. This nine-amino acid peptide blocked migration of melanoma cells to laminin but had no effect on migration to fibronectin. These data suggest that the cell-binding site and migration site on laminin share a common sequence that is unique to laminin.
Iwamoto
Y
Y
Laboratory of Developmental Biology and Anomalies, NIDR, Bethesda, Maryland 20892.
Graf
J
J
Sasaki
M
M
Kleinman
H K
HK
Greatorex
D R
DR
Martin
G R
GR
Robey
F A
FA
Yamada
Y
Y
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D005353:Fibronectins / Q000494:pharmacology; D007797:Laminin / Q000494:pharmacology*; D008545:Melanoma / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D010449:Peptide Mapping; D014407:Tumor Cells, Cultured / Q000503:physiopathology*
D005353:Fibronectins; D007797:Laminin; D010446:Peptide Fragments