Genotyping of human class I alcohol dehydrogenase. Analysis of enzymatically amplified DNA with allele-specific oligonucleotides.
Large inter-individual differences are noted in the susceptibility to alcohol-related problems. Part of this variation may be due to the different isoenzyme patterns of the alcohol-metabolizing enzymes and, consequently, different pharmacokinetics of alcohol degradation. We have used the polymerase chain reaction and oligonucleotide hybridization to amplify and analyze class I alcohol dehydrogenase isoenzyme-specific genomic DNA. The method unambiguously distinguishes between different allelic variants and thus provides a new means of elucidating the alcohol dehydrogenase isoenzyme pattern of humans.
Gennari
K
K
Institut für Biochemie und Molekularbiologie der Universität Bern, Switzerland.
Wermuth
B
B
Muellener
D
D
Ehrig
T
T
von Wartburg
J P
JP
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011089:Polydeoxyribonucleotides / Q000235:genetics*
D004274:DNA, Recombinant; D007527:Isoenzymes; D011089:Polydeoxyribonucleotides; D004247:DNA; D000426:Alcohol Dehydrogenase; D004259:DNA-Directed DNA Polymerase