Measurement of cholesterol in serum by gas chromatography/mass spectrometry at moderate mass resolution, with a nonendogenous cholesterol isomer as internal standard.
We describe a gas chromatography/mass spectrometry method for the quantitative analysis of cholesterol in serum. A structural isomer of cholesterol, 7,(5 alpha)-cholesten-3 beta-ol, is used as an internal standard, its primary advantage being its lesser cost relative to that of a stable-isotope-labeled analog. Analysis of the National Bureau of Standards Certified Reference Serum (SRM 909) was used to validate the method. The results show this method to be highly accurate (bias = -0.6%) and precise (CV = 1.6% between-run, 1.2% within-run). The performance of this method is, therefore, sufficiently good to allow its use as a reference method for determinations of cholesterol in serum.
Kinter
M
M
Department of Pathology, University of Virginia Medical Center, Charlottesville 22908.
Herold
D A
DA
Hundley
J
J
Wills
M R
MR
Savory
J
J
D002784:Cholesterol / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011786:Quality Control; D012015:Reference Standards
C001521:lathosterol; D002784:Cholesterol