title: Comparison between bloodstream and procyclic form trypanosomes for serological diagnosis of African human trypanosomiasis.
abstract: Trypanosoma brucei brucei MiTat 1.2 bloodstream and corresponding procyclic forms, as well as procyclics of T. b. gambiense and T. b. rhodesiense, were fixed, in suspension, using a mixture of 80% acetone and 0.25% (v/v) formalin in saline and used as antigens for diagnosis of African human trypanosomiasis by the indirect immunofluorescent antibody test. T. b. brucei bloodstream forms detected 41/42 and 37/41 parasitologically diagnosed cases of T. b. rhodesiense and T. b. gambiense trypanosomiasis, respectively, while the procyclic stages detected the same number (41/42) of T. b. rhodesiense, but fewer (29/41) T. b. gambiense infections.
authors.affiliation: International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
authors.forename: J M
authors.initials: JM
authors.lastname: Katende
authors.forename: V M
authors.initials: VM
authors.lastname: Nantulya
authors.forename: A J
authors.initials: AJ
authors.lastname: Musoke
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008828:Microbiological Techniques; D014346:Trypanosoma brucei brucei / Q000276:immunology; D014347:Trypanosoma brucei gambiense / Q000276:immunology; D014353:Trypanosomiasis, African / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan