Time-resolved immunofluorometric assay of sex-hormone binding globulin.
A time-resolved immunofluorometric assay (trlFMA) for human sex-hormone binding globulin (SHBG) is described in which antibody-coated tubes or microliter strip-wells and a europium (Eu) chelate-labeled monoclonal antibody are used. The trlFMA sensitivity is similar to that of other SHBG immunoassays, and other analytical variables compare favorably with an SHBG immunoradiometric assay (IRMA) kit and a steroid binding capacity assay: the interassay coefficient of variation (CV) is less than 8% and the intra-assay CV is less than 6% for concentrations between 6 and 200 nmol/L. The reference intervals (means +/- SD) for SHBG concentrations (nmol/L) in serum from 10 men, 10 women, and 10 pregnant women were 23 +/- 12, 65 +/- 39, and 439 +/- 122, respectively. In 14 hirsute women the mean +/- SD serum SHBG concentration (37 +/- 21 nmol/L) was significantly lower (P less than 0.01) than the mean for an age-matched, nonhirsute female comparison group. The trlFMA is technically simple, requires no centrifugation or separation reagent, and takes a counting time of only 1 s. In addition, the Eu-label is nontoxic, presents no waste-disposal problems, and has a long shelf life.
Niemi
S
S
Farmos Diagnostica, Oulunsalo, Finland.
Mäentausta
O
O
Bolton
N J
NJ
Hammond
G L
GL
D000328:Adult; D005063:Europium; D005260:Female; D005456:Fluorescent Dyes; D005470:Fluorometry; D006461:Hemolysis; D006801:Humans; D006949:Hyperlipidemias; D007118:Immunoassay; D008297:Male; D011247:Pregnancy; D011786:Quality Control; D012016:Reference Values; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*
D005456:Fluorescent Dyes; D012738:Sex Hormone-Binding Globulin; D005063:Europium