Release of oligosaccharides possessing reducing-end N-acetylgalactosamine from mucus glycoprotein in Streptomyces sp. OH-11242 culture medium through action of endo-type glycosidase.
A crude enzyme preparation from a culture medium of Streptomyces sp. OH-11242 contained endo-alpha-N-acetylgalactosaminidase activity. The activity could be induced by the addition of purified porcine gastric mucin to the culture medium. Oligosaccharides corresponding to approximately 2-14 glucose units were detected in the culture medium and also in an incubated reaction mixture of crude enzyme preparation and mucus glycoprotein. The resulting product with N-acetylgalactosamine at the reducing terminal implied the presence of a new type of endo-glycosidase liberating not only Gal beta 1-3GalNAc but also other larger oligosaccharides by hydrolysis of the O-glycosidic linkage between GalNAc and Ser (Thr).
Iwase
H
H
Department of Biochemistry, School of Medicine, Kitasato University, Kanagawa, Japan.
Ishii
I
I
Ishihara
K
K
Tanaka
Y
Y
Omura
S
S
Hotta
K
K
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005753:Gastric Mucosa; D006023:Glycoproteins / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism*; D006868:Hydrolysis; D009077:Mucins / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology*; D013552:Swine; D048809:alpha-N-Acetylgalactosaminidase
D003470:Culture Media; D006023:Glycoproteins; D009077:Mucins; D009844:Oligosaccharides; C047874:gastric mucus glycoproteins; D006596:Hexosaminidases; D048809:alpha-N-Acetylgalactosaminidase