Induction of a latency period in the time-course of phospholipase A2 action on dipalmitoylphosphatidylcholine liposomes in the gel phase.
The hydrolysis of dipalmitoylphosphatidylcholine liposomes by porcine pancreatic phospholipase A2 was studied at 31 degrees C, i.e., with the substrate in the gel phase. Addition of delipidated bovine serum albumin to the assay medium induces the appearance of a latency phase in the time course of the enzymatic action. The lag period can be abolished by addition of free palmitic acid whereas no reversal by lysolecithin is found. The generation of a latency period by albumin appears to be due to its ability to sequester the palmitic acid newly released by the phospholipase A2 catalysis. Thus, the nascent fatty acid seems to be an essential activator of the enzymatic process.
González-Martínez
M T
MT
Dept. Bioquimica, Centro de Investigación y de Estudios Avanzados del I.P.N., México D.F., Mexico.
Fernández
M S
MS
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D005782:Gels; D006868:Hydrolysis; D007700:Kinetics; D008081:Liposomes; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013552:Swine
D005782:Gels; D008081:Liposomes; D010169:Palmitic Acids; D015060:1,2-Dipalmitoylphosphatidylcholine; D019308:Palmitic Acid; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2