The organ distribution of F protein in the mouse.
F protein is of unknown function and found principally in the liver, with smaller amounts detectable by gel diffusion and immunostaining in kidney and brain. By taking advantage of a newly developed radioimmunoassay, capable of detecting down to 1 ng ml-1 of F protein, the organ distribution of this molecule in the mouse was re-examined. F protein was not confined to the previously known tissues and indeed was detected at different levels in every organ studied. The highest concentration (6-10 mg g-1 wet tissue) was found in the liver, but significant amounts (2-25 micrograms g-1) were also found in kidney, heart, and brain. Lower quantities were detectable in all other tissues studied. The presence of F protein in the thymus, and the lack of tolerance to this in bone marrow chimaeras between mice of different F protein types, places certain constraints on the amount and/or location of a protein required for tolerization.
Griffiths
J A
JA
Department of Zoology and Cell Biology, University College, London, UK.
Oliveira
D B
DB
D000818:Animals; D001666:Binding Sites, Antibody; D001923:Brain Chemistry; D007519:Isoantigens / Q000032:analysis* / Q000276:immunology; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000032:analysis; D011863:Radioimmunoassay; D014020:Tissue Extracts / Q000032:analysis* / Q000276:immunology
D007519:Isoantigens; D014020:Tissue Extracts; C030497:liver specific F antigen