Biochemical and autoradiographic study of cerebral protein synthesis with [18F]-and [14C]fluorophenylalanine.
Fluorine-18-labeled ortho or para isomers of L-fluorophenylalanine were used in double-label experiments together with L-[3H]phenylalanine for amino acid incorporation into cerebral proteins of Mongolian gerbil brain. It was demonstrated by qualitative regional comparison of the 18F and 3H autoradiographic images that L-p-[18F]fluorophenylalanine is incorporated into proteins and exhibits a regional cerebral protein synthesis pattern. To a minor extent, L-p-fluorophenyl[3-14C]alanine and L-o-[18F]fluorophenylalanine are hydroxylated in vivo to form labeled tyrosine or tyrosine analogues that are incorporated into cerebral proteins as well. The advantage and validity of the application of L-p-[18F]fluorophenylalanine with positron emission tomography for noninvasive studies of cerebral protein synthesis in humans are evaluated on the basis of an experimental animal approach.
Bodsch
W
W
Max-Planck-Institut für Neurologische Forschung, Abteilung für Experimentelle Neurologie, Köln, F.R.G.
Coenen
H H
HH
Stöcklin
G
G
Takahashi
K
K
Hossmann
K A
KA
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes; D055598:Chemical Phenomena; D002621:Chemistry; D005462:Fluorine Radioisotopes; D005849:Gerbillinae; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D010135:p-Fluorophenylalanine / Q000378:metabolism*
D002250:Carbon Radioisotopes; D005462:Fluorine Radioisotopes; D009419:Nerve Tissue Proteins; D014443:Tyrosine; D010649:Phenylalanine; D010135:p-Fluorophenylalanine