Two adjacent mutations at position 12 activate the K-ras2 oncogene of a human mammary tumor cell line.
Among 10 human mammary tumor cell lines analyzed for transforming genes by transfection of NIH 3T3 cells, one carcinoma cell line, H-466B, established from an ascitic effusion of a woman with an adenocarcinoma of the breast was scored as positive. The transforming gene was identified as the K-ras2 oncogene. Nucleotide sequencing of exons 1 and 2 of the activated gene revealed two adjacent G----T transversions at the first and second position in codon 12 leading to the replacement of the normally encoded glycine by a phenylalanine. Since the phenylalanine substitution had never been observed in any type of tumor, this raises the question about the frequency as well as the cell type specificity of this K-ras2 activation in mammary tumors.
Prosperi
M T
MT
Laboratoire d'Oncogenèse Moléculaire, Institut Curie, Paris, France.
Even
J
J
Calvo
F
F
Lebeau
J
J
Goubin
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003062:Codon; D005260:Female; D005786:Gene Expression Regulation; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D014162:Transfection
D003062:Codon