Induction of sister-chromatid exchanges by procarcinogens in metabolically competent Chinese hamster epithelial liver cells.
An epithelial cell strain has been established from the livers of male Chinese hamsters (CHEL cells). These cells, which proliferate in culture and retain their metabolic enzymatic activities during several subcultures, were used in a sister-chromatid exchange assay to evaluate the effectiveness of polycyclic aromatic hydrocarbons (PAHs), aflatoxin B1 (AFB1) and cyclophosphamide (CP). The results obtained demonstrate that CHEL cells are metabolically competent to activate different classes of procarcinogens into biologically active metabolites. Moreover, they showed a selective capacity to discriminate chemical carcinogens from noncarcinogens. Thus, the CHEL cell system appears to be a promising alternative to the short-term tests that include cell-free rodent liver homogenate to evaluate new promutagens and/or procarcinogens.
De Salvia
R
R
Centro di Genetica Evoluzionistica C.N.R., Univ. La Sapienza, Rome, Italy.
Meschini
R
R
Fiore
M
M
Polani
S
S
Palitti
F
F
Carluccio
M A
MA
Turchi
G
G
D000818:Animals; D001711:Biotransformation; D002273:Carcinogens / Q000493:pharmacokinetics; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D012854:Sister Chromatid Exchange
D002273:Carcinogens; D009153:Mutagens