Sandwich-type ELISA for free and bound secretory component in human biological fluids.
Three sandwich-type enzyme-linked immunosorbent assays (ELISA) are described for the measurement of free secretory component (SC) and SC bound to IgA (S-IgA) or IgM (S-IgM). These assays do not require preliminary fractionation of the biological fluids to be tested. The specificity of the assays is achieved with monoclonal antibodies specific for free SC (855 SC) and for SC bound to IgA or IgM (8545 SA). The amount of the three SC molecules in various biological fluids is reported. We demonstrate the presence of low levels of free SC in most of these fluids, including normal serum. Moreover our results suggest that S-IgM in serum may result from a non-covalent association between serum IgM and free SC.
Vincent
C
C
Laboratoire d'Immunologie, INSERM U80, CNRS UA 1177, UCLB, Hôpital E. Herriot, Lyon, France.
Revillard
J P
JP
D000328:Adult; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007128:Immunoglobulin Fragments / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D012635:Secretory Component / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory; D007128:Immunoglobulin Fragments; D007075:Immunoglobulin M; D012635:Secretory Component