Genetic mapping of nuclear mucidin resistance mutations in Saccharomyces cerevisiae. A new pdr locus on chromosome II.
In the yeast Saccharomyces cerevisiae, two nuclear pleiotropic drug resistance mutations pdr3-1 (former designation mucPR) and pdr3-2 (former designation DRI9/T7) have been selected as resistant to mucidin and as resistant to chloramphenicol plus cycloheximide, respectively. The pdr3 mutations were found not to affect the plasma membrane ATPase activity measured in a crude membrane fraction. Meiotic mapping using strains with standard genetic markers revealed that mutation pdr3-1 is centromere linked on the left arm of chromosome II at a distance of 5.9 +/- 3.3 cM from its centromere and 11.6 +/- 3.1 cM from the marker pet9. The centromere linked pdr3-2 mutation exhibited also genetic linkage to pet9 with a map distance of 9.8 +/- 3.2 cM. These results indicate that pdr3-1 and pdr3-2 are alleles of the same pleiotropic drug resistance locus PDR3 which is involved in the control of the plasma membrane permeability in yeast.
Subik
J
J
Food Research Institute, Bratislava, Czechoslovakia.
Ulaszewski
S
S
Goffeau
A
A
D000475:Alkenes / Q000494:pharmacology; D000483:Alleles; D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial; D005231:Fatty Acids, Unsaturated; D005800:Genes, Fungal; D008040:Genetic Linkage; D008689:Methacrylates; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D000073739:Strobilurins
D000475:Alkenes; D005231:Fatty Acids, Unsaturated; D008689:Methacrylates; D000073739:Strobilurins; C008556:mucidin