Association between biochemical and physiological properties in single motor units.
Motor units from the cat tibialis posterior muscle were examined for an association between physiological and biochemical properties. Functionally isolated motor units were categorized on the basis of their physiological properties. This was followed by quantitative microbiochemical analysis of single muscle fibers from each unit, identified in cross sections using the glycogen-depletion method. The activities of malate dehydrogenase and beta-hydroxyacyl-CoA dehydrogenase distinguished between fatigable (type FF) and fatigue-resistant (types FR and S) units. The activities of both lactate dehydrogenase and adenylokinase were higher in fast- than in slow-contracting units. Cluster analyses, based on both physiological and biochemical properties or on biochemical properties alone, produced groupings identical to types FF, FR, and S. The association between physiological and biochemical properties substantiates the idea that biochemically distinct groups of motor units correspond to physiologically identifiable groups.
Hamm
T M
TM
Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ 85013.
Nemeth
P M
PM
Solanki
L
L
Gordon
D A
DA
Reinking
R M
RM
Stuart
D G
DG
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000032:analysis; D000263:Adenylate Kinase / Q000032:analysis; D000818:Animals; D002415:Cats; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D009046:Motor Neurons / Q000201:enzymology / Q000502:physiology*; D009119:Muscle Contraction; D009132:Muscles / Q000294:innervation*
D015094:3-Hydroxyacyl CoA Dehydrogenases; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D000263:Adenylate Kinase