CAMP-dependent protein kinase of sea urchin sperm phosphorylates sperm histone H1 on a single site.
The phosphorylation of sperm specific histone H1 in the sea urchin Strongylocentrotus purpuratus occurs both in vivo and in vitro on a single serine site in the sequence Arg-Lys-Gly-Ser(P)-Ser-Asn-Ala-Arg. This is a preferred sequence for cAMP-dependent protein kinase. The in vitro phosphorylation is completely dependent on cAMP and is inhibited by the peptide protein kinase inhibitor. The protein kinase inhibitor H-8 blocks the in vivo phosphorylation of H1 without damaging motility, the acrosome reaction or the ability of sperm to fuse with and activate eggs.
Porter
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Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093.
Moy
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Vacquier
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VD
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D006657:Histones / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D011494:Protein Kinases / Q000032:analysis / Q000378:metabolism*; D012617:Sea Urchins; D013045:Species Specificity; D013094:Spermatozoa / Q000201:enzymology*
D006657:Histones; D011494:Protein Kinases