Influence of rosmarinic acid on opsonization and intracellular killing of Escherichia coli and Staphylococcus aureus by porcine and human polymorphonuclear leucocytes.
The influence of rosmarinic acid on the function of porcine and human polymorphonuclear leucocytes was tested. Rosmarinic acid inhibited the chemiluminescence of PMNL, induced by preopsonized Zymosan or phorbol myristate acetate. The killing of Escherichia coli was inhibited by rosmarinic acid at a concentration of 2 mM, but not that of Staphylococcus aureus. The inhibition of the killing was due to an impaired opsonization, caused by an adverse influence of rosmarinic acid on complement activation. Direct effects of rosmarinic acid on the killing mechanisms of PMNL were not observed.
Verweij-van Vught
A M
AM
Department of Medical Microbiology, School of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
Appelmelk
B J
BJ
Groeneveld
A B
AB
Sparrius
M
M
Thijs
L G
LG
MacLaren
D M
DM
D000818:Animals; D002934:Cinnamates / Q000494:pharmacology*; D003167:Complement Activation / Q000187:drug effects; D053630:Depsides; D004926:Escherichia coli; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D009895:Opsonin Proteins / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects*; D013211:Staphylococcus aureus; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology; D000097123:Rosmarinic Acid
D002934:Cinnamates; D053630:Depsides; D009895:Opsonin Proteins; D010100:Oxygen; D013755:Tetradecanoylphorbol Acetate; D015054:Zymosan; D000097123:Rosmarinic Acid