[Biological significance of the endogenous secretion of insulin in response to breakfast in diabetic children].
In 20 insulin dependent diabetics and in eight age-matched healthy children, we have measured basally HbA1 and the plasma lipid profile; and both basally and after a standard breakfast of 400 cal, blood glucose, C-peptide (CPR), immunoreactive glucagon (IRG), growth hormone and nonesterified fatty acids (NEFA). No difference was found in the lipid profile of diabetic and non diabetic children. In diabetics basal blood glucose was significantly correlated to both basal C-peptide (r = 0.5332, p less than 0.05) and to the ratio C-peptide/glucagon (r = 0.8563, p less than 0.01). The C-peptide response to the breakfast was accompanied of a significant increase in IRG and NEFA in diabetics, while in non-diabetics there was no change in IRG and a significant decrease in NEFA levels. Diabetic children with basal C-peptide levels higher than 0.6 ng/ml had lower blood glucose levels (187 +/- 43 vs 315 +/- 20 mg/dl, p less than 0.02), but no difference in blood glucose or any other parameter was observed as a function of the increase in CPR after breakfast. From these results we conclude that the evaluation of residual B-cell function in diabetic children by measuring the C-peptide response to a provocative stimulus is not more informative than the single basal measurement of C-peptide and does not have an additional biological significance, at least in hyperglycaemic insulin dependent diabetic children.
Gualda
P
P
Departamento de Fisiología y Bioquímica, Hospital Clínico Universitario, Facultad de Medicina, Universidad de Granada, España.
Fernández
J M
JM
Rubio
R
R
Osuna
J I
JI
Molina-Font
J A
JA
Castillo
M
M
D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000097:blood*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005502:Food; D005934:Glucagon / Q000097:blood; D006442:Glycated Hemoglobin / Q000378:metabolism; D013006:Growth Hormone / Q000097:blood*; D006801:Humans; D007328:Insulin / Q000502:physiology*; D008297:Male
D001786:Blood Glucose; D002096:C-Peptide; D005230:Fatty Acids, Nonesterified; D005934:Glucagon; D006442:Glycated Hemoglobin; D013006:Growth Hormone; D007328:Insulin
Significación biológica de la secreción endógena de insulina, en respuesta al desayuno en niños diabéticos.