Description of the molecular mechanism of cooperativity in human hemoglobin cannot be limited to a first-order free energy coupling concept.
Studies of the linkage between ligand binding and subunit assembly of oligomeric proteins have extensively used the concept of free energy coupling. The "order" of these free energy couplings was introduced [Weber, G. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 7098-7102] as the number of subunits that must be liganded to alter specific intersubunit interactions. This concept dictates that the ligation of fewer subunits has no effect, but once the order number of subunits becomes ligated, the specific intersubunit interaction energy between those particular subunits is completely eliminated. Weber's report claims that the free energy coupling between oxygen binding and the dimer-tetramer subunit assembly in stripped human hemoglobin A is "first order". This conclusion is based on the analysis of a set of previously published equilibrium constants [Mills, F. C., Johnson, M. L., & Ackers, G. K. (1976) Biochemistry 15, 5350-5362]. I subsequently reported that the original experimental data, from which the equilibrium constants were derived, are consistent with both the first-order and "second-order" free energy coupling concepts [Johnson, M. L. (1986) Biochemistry 25, 791-797]. I also demonstrated that more precise recent experimental data [Chu, A. H., Turner, B. W., & Ackers, G. K. (1984) Biochemistry, 23, 604-617] are consistent with both the first-order and second-order free energy coupling concepts. A recent article [Weber, G. (1987) Biochemistry 26, 331-332] disagrees that the oxygen-binding data for human hemoglobin A are consistent with a second-order model.(ABSTRACT TRUNCATED AT 250 WORDS)
Johnson
M L
ML
Department of Pharmacology, University of Virginia School of Medicine, Charlottesville 22908.
D006441:Hemoglobin A / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D046911:Macromolecular Substances; D008962:Models, Theoretical; D010108:Oxyhemoglobins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D013816:Thermodynamics
D008024:Ligands; D046911:Macromolecular Substances; D010108:Oxyhemoglobins; D011506:Proteins; D006441:Hemoglobin A; C029774:oxyhemoglobin A