Inhibition of measles virus-induced cell-cell fusion with a monoclonal antibody directed against the haemagglutinin.
A neutralizing monoclonal antibody (C26-15) against the haemagglutinin (H protein) of measles virus was generated which caused cell-cell fusion inhibition in cultures of measles virus-infected cells. It was shown that this phenomenon coincided with a down-regulation of the expression of both the H protein and the fusion (F) protein. We also showed cell-cell fusion inhibition with a polyclonal rabbit serum directed against Tween-ether inactivated measles virus, which did not contain biologically active antibodies against the F protein. Cell-cell fusion inhibition caused by anti-H antibodies is distinct from cell-cell fusion inhibition induced by a direct interaction of anti-F antibody with the F protein in the membrane of infected cells. Since both mechanisms may also be involved in the in vivo situation, the exclusive role for the generation of anti-F antibody to prevent virus spread by cell-cell fusion in vivo is questioned. It is speculated that the observed down-regulation of both glycoproteins may lead to a less efficient killing of infected cells by cytotoxic T-lymphocytes, which may constitute an alternative explanation for the insufficient protection after vaccination with an inactivated measles vaccine.
de Vries
P
P
National Institute of Public Health and Environmental Hygiene, Bilthoven, The Netherlands.
Van Binnendijk
R S
RS
Van der Marel
P
P
Beuvery
E C
EC
Van Wezel
A L
AL
Voorma
H O
HO
Uytedehaag
F G
FG
Osterhaus
A D
AD
D000594:Amino Acid Oxidoreductases; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology; D002459:Cell Fusion; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D050961:Glycine Decarboxylase Complex H-Protein; D050841:Glycine Dehydrogenase (Decarboxylating); D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008459:Measles virus / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D009124:Muscle Proteins / Q000276:immunology; D010088:Oxidoreductases; D011506:Proteins; D014709:Vero Cells / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D050961:Glycine Decarboxylase Complex H-Protein; D006389:Hemagglutinins, Viral; C488911:Hpd protein, mouse; D009124:Muscle Proteins; D011506:Proteins; D010088:Oxidoreductases; D000594:Amino Acid Oxidoreductases; D050841:Glycine Dehydrogenase (Decarboxylating)