Properties of odour-binding glycoproteins from rat olfactory epithelium.
The specific membrane glycoproteins with high affinity for camphor and decanal were isolated from rat olfactory epithelium. Antibodies to these glycoproteins inhibited both the electroolfactogram and the binding of odorants. The enzyme immunoassay has shown these glycoproteins to be present in the olfactory epithelium of rat, mouse, guinea-pig and hamster but not in that of frog and carp. The molecular mass of the odour-binding glycoproteins from rat olfactory epithelium solubilized by Triton X-100 was approx. 140 kDa. They consisted of two subunits (88 and 55 kDa). The 88 kDa subunit was capable of binding odorants. The data obtained suggest that the glycoproteins isolated have some properties that make them plausible candidates for olfactory receptor molecules.
Fesenko
E E
EE
Institute of Biological Physics, Academy of Sciences, Pushchino, Moscow Region, U.S.S.R.
Novoselov
V I
VI
Bystrova
M F
MF
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002164:Camphor / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000032:analysis; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008970:Molecular Weight; D009812:Odorants; D009831:Olfactory Mucosa / Q000032:analysis* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D011984:Sensory Receptor Cells / Q000032:analysis* / Q000378:metabolism; D013045:Species Specificity
D000447:Aldehydes; D000941:Antigens; D002352:Carrier Proteins; D006023:Glycoproteins; D007074:Immunoglobulin G; C021170:decanaldehyde; D002164:Camphor