Alkylacylglycerol molecular species in the glycosylinositol phospholipid membrane anchor of bovine erythrocyte acetylcholinesterase.
Bovine erythrocyte acetylcholinesterase, a glycosylinositol phospholipid anchored membrane enzyme, was digested with phosphatidylinositol-specific phospholipase C and the released glycerol-containing moieties were identified and quantitated. About 96% of the total was alkylacylglycerol, of which sn-1-stearyl-2-stearoylglycerol, sn-1-stearyl-2-oleoylglycerol and sn-1-oleyl-2-stearoylglycerol accounted for 69%, 13% and 10%, respectively. These alkylacylglycerols are in marked contrast to the exclusively diacylglycerol species present in phosphatidylinositol from bovine erythrocyte membranes. This difference suggests that assembly of the membrane anchor of Ebo AChE involves a selected cellular pool of diradylglycerols.
Roberts
W L
WL
Department of Pharmacology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106.
Myher
J J
JJ
Kuksis
A
A
Rosenberry
T L
TL
D000110:Acetylcholinesterase / Q000097:blood*; D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005227:Fatty Acids / Q000097:blood; D005989:Glycerides / Q000097:blood*; D005995:Glyceryl Ethers / Q000097:blood; D008563:Membrane Lipids / Q000097:blood*; D010716:Phosphatidylinositols / Q000097:blood*; D010738:Type C Phospholipases / Q000378:metabolism
D005227:Fatty Acids; D005989:Glycerides; D005995:Glyceryl Ethers; D008563:Membrane Lipids; D010716:Phosphatidylinositols; D000110:Acetylcholinesterase; D010738:Type C Phospholipases