title: Protein phosphorylation and activation of human platelets by sodium fluoride.
abstract: The ability of sodium fluoride (NaF) and thrombin to stimulate aggregation and protein phosphorylation in intact human platelets was measured and compared. When platelets were stimulated by NaF, phosphorylation of the 20 KDa protein was transient and after 5-10 min returned to the same level as that of unstimulated cells. On the other hand, 47 KDa protein was slowly phosphorylated without obvious dephosphorylation. The slow activation of the 47 KDa protein phosphorylation correlated well with the time required for the aggregation and secretion. Phosphoamino acid analysis showed that the phosphorylated amino acids of the 47 KDa protein from platelets activated by NaF and thrombin were slightly different. These results suggest that different stimuli may lead to the same protein phosphorylation by different biochemical mechanisms of action.
authors.affiliation: Department of Biochemistry, Fukui Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Yanagi
authors.forename: H
authors.initials: H
authors.lastname: Yamamura
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D004590:Electrophoresis, Paper; D006801:Humans; D010750:Phosphoproteins; D010766:Phosphorylation; D010974:Platelet Aggregation / Q000187:drug effects; D012969:Sodium Fluoride / Q000494:pharmacology*; D013917:Thrombin / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D001798:Blood Proteins; D010750:Phosphoproteins; C039077:platelet protein P47; D012969:Sodium Fluoride; D013917:Thrombin