Giant polypeptides of skeletal muscle titin: sedimentation equilibrium in guanidine hydrochloride.
Titin has been purified from rabbit skeletal myofibrils in guanidine hydrochloride by gel filtration on Sephacryl S-1000. The sedimentation coefficients of denatured titin in 6 M guanidine hydrochloride are highly concentration dependent and follow the equation: 1/S = 0.241 + 0.302 A280. Equilibrium sedimentation studies indicate that the average molecular weight of titin polypeptides is 2.4 to 2.6 X 10(6).
Kurzban
G P
GP
Clayton Foundation Biochemical Institute, Department of Chemistry, Austin, Texas.
Wang
K
K
D000818:Animals; D002850:Chromatography, Gel; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D019791:Guanidine; D006146:Guanidines; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification*; D009132:Muscles / Q000032:analysis*; D011489:Protein Denaturation; D011494:Protein Kinases; D011817:Rabbits; D014461:Ultracentrifugation
D064211:Connectin; D006146:Guanidines; D009124:Muscle Proteins; D011494:Protein Kinases; D019791:Guanidine