Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
Expression of the c-myc gene in human breast lesions and in adjacent normal tissue was studied by immunohistochemical analysis. The previously described monoclonal antibody Myc1-9E10 (1) which recognizes the p62 c-myc protein was used in paraffin tissue sections. A total of 101 cases of breast disease examined included 38 simple and complex cystic disease, 18 simple and hyperplastic fibroadenomas, 36 ductal and lobular carcinomas and 9 in situ carcinomas. Whereas the adjacent normal tissue was slightly positive, 25 out of 38 cystic disease, 7 out of 18 fibroadenoma, 36 out of 36 carcinoma and 9 out of 9 in situ carcinoma specimens showed moderate to high levels of p62 c-myc expression as indicated by staining intensity. These results suggest that the c-myc protein may play a role in breast neoplasia.
Spandidos
D A
DA
Beatson Institute for Cancer Research, Bearsden, Glasgow, Scotland, U.K.
Pintzas
A
A
Kakkanas
A
A
Yiagnisis
M
M
Mahera
H
H
Patra
E
E
Agnantis
N J
NJ
D000230:Adenocarcinoma / Q000032:analysis / Q000235:genetics; D000911:Antibodies, Monoclonal; D001941:Breast Diseases / Q000235:genetics* / Q000378:metabolism; D001943:Breast Neoplasms / Q000032:analysis / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes
D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc