title: Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
abstract: Expression of the c-myc gene in human breast lesions and in adjacent normal tissue was studied by immunohistochemical analysis. The previously described monoclonal antibody Myc1-9E10 (1) which recognizes the p62 c-myc protein was used in paraffin tissue sections. A total of 101 cases of breast disease examined included 38 simple and complex cystic disease, 18 simple and hyperplastic fibroadenomas, 36 ductal and lobular carcinomas and 9 in situ carcinomas. Whereas the adjacent normal tissue was slightly positive, 25 out of 38 cystic disease, 7 out of 18 fibroadenoma, 36 out of 36 carcinoma and 9 out of 9 in situ carcinoma specimens showed moderate to high levels of p62 c-myc expression as indicated by staining intensity. These results suggest that the c-myc protein may play a role in breast neoplasia.
authors.affiliation: Beatson Institute for Cancer Research, Bearsden, Glasgow, Scotland, U.K.
authors.forename: D A
authors.initials: DA
authors.lastname: Spandidos
authors.forename: A
authors.initials: A
authors.lastname: Pintzas
authors.forename: A
authors.initials: A
authors.lastname: Kakkanas
authors.forename: M
authors.initials: M
authors.lastname: Yiagnisis
authors.forename: H
authors.initials: H
authors.lastname: Mahera
authors.forename: E
authors.initials: E
authors.lastname: Patra
authors.forename: N J
authors.initials: NJ
authors.lastname: Agnantis
mesh_terms: D000230:Adenocarcinoma / Q000032:analysis / Q000235:genetics; D000911:Antibodies, Monoclonal; D001941:Breast Diseases / Q000235:genetics* / Q000378:metabolism; D001943:Breast Neoplasms / Q000032:analysis / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes
chemical_list: D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc