Regulation of reductive production of succinate under anaerobic conditions in baker's yeast.
When baker's yeast grown aerobically on ethanol as a carbon source was anaerobically cultured in a medium containing glucose, the activity of a cytoplasmic fumarate reductase irreversibly catalyzing the conversion of fumarate to succinate increased, reaching about 3 times the original activity after 12 h, while the activity of succinate dehydrogenase was almost lost after 10 h. These results indicate that the citrate cycle is partially modified to become a reductive pathway leading to succinate during the anaerobic cultivation. In non-proliferating cells grown anaerobically on glucose, the rates of accumulating succinate and pyruvate were decreased and increased, respectively, with increasing concentrations of L-aspartate or NH4Cl in the medium containing glucose as a substrate. These changes were accompanied with increase in the cellular content of aspartate, an inhibitor of pyruvate carboxylase that is involved in supplying the intermediates of the citrate cycle, and pyruvate, a substrate of the enzyme. The aminotransferase inhibitor, aminooxyacetate, prevented the changes in succinate accumulation and cellular aspartate following the addition of NH4Cl. The addition of L-glutamate caused a marked increase in the rate of succinate accumulation without changing the cellular content of aspartate. Neither L-glutamate nor L-aspartate had the ability to produce succinate. The rate of glucose consumption was not changed upon adding these nitrogen compounds. Similar findings were also observed in experiments using proliferating cells. This report presents evidence that in cells containing a large amount of the fumarate reductase, the production of succinate from glucose is regulated by the cellular level of aspartate through the pyruvate carboxylase reaction and that glutamate regulates the succinate production by a mechanism distinct from that involved in the regulation by L-aspartate.
Muratsubaki
H
H
Department of Clinical Biochemistry, Faculty of Health Science, Kyorin University, Tokyo.
D000596:Amino Acids / Q000502:physiology; D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000643:Ammonium Chloride / Q000494:pharmacology; D000693:Anaerobiosis; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013386:Succinates / Q000378:metabolism*
D000596:Amino Acids; D013386:Succinates; D000643:Ammonium Chloride; D000625:Aminooxyacetic Acid; D013385:Succinate Dehydrogenase