Immunohistochemical and immunoblot analyses of collagens in the developing fibrocartilage in the glans penis of the rat.
The distal segment of the os penis of rats is a fibrocartilage whose development is dependent on androgens. Electrophoretic and immunoblot analysis indicated that the fibrocartilage contained both type I and type II collagens. Immunohistochemically, type I collagen was detected in the fibrous matrix of the distal segment in all the stages examined. Type II collagen was detected first at 4 weeks in the extracellular matrix surrounding the mature chondrocytes distributed sparsely as single cells or small clusters among immature chondroblasts. The clusters reactive with anti-type II collagen serum increased in size and number at 6 weeks, and almost all the region of the distal segment became reactive with anti-type II collagen serum at 8 weeks.
Murakami
R
R
Biological Institute, Faculty of Science, Yamaguchi University, Japan.
D000818:Animals; D002356:Cartilage / Q000032:analysis*; D003094:Collagen / Q000032:analysis* / Q000145:classification; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D010413:Penis / Q000032:analysis*; D051381:Rats / Q000378:metabolism*; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains
D003094:Collagen