Iodine-125 cholesteryl iopanoate for measuring extent of atherosclerosis in rabbits.
Rabbits rendered atherosclerotic by mechanical aortic de-endothelialization and 6 wk of cholesterol feeding were administered estradiol-17 beta-cypionate, an anti-atherogenic agent in rabbits. These animals were compared to a similar, untreated group and control animals fed a regular non-atherogenic diet. Iodine-125 cholesteryl iopanoate ([125I]Cl), a nonhydrolyzable cholesteryl ester derivative, was administered intravenously at regular intervals throughout the study. Six days after the last dose of [125I]Cl, the animals were scanned with a gamma camera. After animals were killed, tissue distribution of the 125I radioactivity showed a significant decrease of [125I]CI accumulation in the aorta of estrogen-treated as compared to untreated, cholesterol-fed animals. However, the accumulation of [125I]CI in the aortas was insufficient to accurately define the presence of atheroma by gamma camera scintigraphy.
DeGalan
M R
MR
Department of Pharmacology, University of Michigan Medical School, Ann Arbor 48109-0626.
Schwendner
S W
SW
Skinner
R W
RW
Longino
M A
MA
Gross
M
M
Counsell
R E
RE
D000818:Animals; D001011:Aorta / Q000000981:diagnostic imaging; D001161:Arteriosclerosis / Q000097:blood / Q000000981:diagnostic imaging* / Q000188:drug therapy; D001853:Bone Marrow / Q000000981:diagnostic imaging; D002788:Cholesterol Esters; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000031:analogs & derivatives / Q000627:therapeutic use; D005260:Female; D007457:Iodine Radioisotopes; D008055:Lipids / Q000097:blood; D011817:Rabbits; D011877:Radionuclide Imaging; D013154:Spleen / Q000000981:diagnostic imaging; D013961:Thyroid Gland / Q000000981:diagnostic imaging
D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D007457:Iodine Radioisotopes; D008055:Lipids; D004958:Estradiol; C007630:estradiol 17 beta-cypionate; C039133:cholesteryl iopanoate