Dominant cataract and recessive specific-locus mutations detected in offspring of procarbazine-treated male mice.
The induction of dominant cataract mutations by procarbazine was studied concomitantly with the induction of specific-locus mutations in treated male mice. The most effective dose in the specific-locus test, 600 mg/kg of procarbazine, and a fractionated dose of 5 X 200 mg/kg were used. The frequencies of dominant cataract mutations were higher, but not significantly different from the historical control. The ratio between the number of recovered specific-locus and dominant cataract mutations was in accordance with that found in our experiments with gamma-rays (Ehling et al., 1982; Kratochvilova, 1981) or in experiments with ethylnitrosourea (Favor, 1986). A total of 3 dominant cataract mutations were recovered in the offspring of procarbazine-treated spermatogonial stem cells. Two mutations had complete penetrance while the third exhibited a reduced penetrance of approximately 70%. The viability and fertility of the heterozygotes of all 3 mutations were not affected. Only 1 mutation was shown to be viable as a homozygote.
Kratochvilova
J
J
Institut für Säugetiergenetik, Gesellschaft für Strahlen- und Umweltforschung, Neuherberg, F.R.G.
Favor
J
J
Neuhäuser-Klaus
A
A
D000014:Abnormalities, Drug-Induced / Q000235:genetics*; D000818:Animals; D002386:Cataract / Q000139:chemically induced* / Q000151:congenital / Q000235:genetics; D004843:Epistasis, Genetic; D005298:Fertility / Q000528:radiation effects; D005799:Genes, Dominant / Q000187:drug effects*; D005808:Genes, Recessive / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011344:Procarbazine / Q000633:toxicity*; D013093:Spermatogonia / Q000187:drug effects
D011344:Procarbazine