Dietary restriction, cell proliferation and carcinogenesis: a preliminary study.
Four groups of female Swiss Webster mice were given either laboratory chow or a purified (semi-synthetic) diet (AIN-76A) either ad libitum or at 75% of the ad libitum rate for about 30 days. Three tissues, the crypt cells of the jejunum, the dermis and the basal epithelial cells of the esophagus were investigated using [3H]thymidine labelling and by counting mitoses; four other tissues, the alveolar cells of the mammary gland, the crypt cells of the duodenum and colo-rectum, and the transitional cells of the urinary bladder were examined using [3H]thymidine labelling only. In each case dietary restriction led to a reduction of cellular proliferation assessed by these indices. The potential of the approach for the study of the effects of dietary modification on the induction of cancer is discussed.
Lok
E
E
Toxicology Research Division, Bureau of Chemical Safety, Health & Welfare, Canada, Ottawa, Ontario.
Nera
E A
EA
Iverson
F
F
Scott
F
F
So
Y
Y
Clayson
D B
DB
D000818:Animals; D002455:Cell Division; D004038:Diet, Reducing; D005260:Female; D051379:Mice; D009374:Neoplasms, Experimental / Q000209:etiology*; D013936:Thymidine / Q000378:metabolism
D013936:Thymidine