title: Adenosine effects on hormone-stimulated steroidogenesis in isolated bovine adrenal zona fasciculata cells.
abstract: In isolated bovine adrenal zona fasciculata cells, the use of adenosine deaminase to remove endogenous adenosine had no effect on basal or angiotensin II-stimulated steroidogenesis but enhanced ACTH1-24-stimulated steroidogenesis over the entire dose response range without appreciable change in potency of ACTH1-24. 8-Phenyl-theophylline, an adenosine antagonist, mimicked all of the actions of adenosine deaminase. High concentrations (greater than 1 microM) of N6-phenylisopropyl-adenosine (PIA) increased basal, angiotensin II and cyclic AMP-stimulated steroidogenesis, whilst inhibiting the ACTH1-24-stimulated condition. PIA also increased the potency of angiotensin II approx 20-fold. These observations are consistent with the possibility that adenosine exerts effects on two different signalling systems within zona fasciculata cells.
authors.affiliation: Department of Biochemistry, University of Newcastle-upon-Tyne, England.
authors.forename: W J
authors.initials: WJ
authors.lastname: Koper
authors.forename: S J
authors.initials: SJ
authors.lastname: Yeaman
authors.forename: R C
authors.initials: RC
authors.lastname: Honnor
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000243:Adenosine Deaminase / Q000494:pharmacology; D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003366:Cosyntropin / Q000494:pharmacology*; D006854:Hydrocortisone / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007700:Kinetics; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D003366:Cosyntropin; D010660:Phenylisopropyladenosine; D013806:Theophylline; C028322:8-phenyltheophylline; D000243:Adenosine Deaminase; D000241:Adenosine; D006854:Hydrocortisone