Hybridoma cultivation in defined serum-free media: growth-supporting substances. II. Insulin, other hormones, and growth factors.
The effects of insulin and hydrocortisone on growth of hybridomas in serum-free medium were studied. The optimum growth-stimulating concentration of insulin was 10 micrograms/ml. Omission of insulin resulted in substantially decreased growth (23-53% of the cells growing 3 days with insulin). The optimum growth-supporting concentration of hydrocortisone was 2 ng/ml. Hybridoma cells growing 3 days without hydrocortisone represented about 80% of the cells growing with hydrocortisone. Hormones testosterone, triiodothyronine, and prostaglandin F2 alpha as well as growth factors EGF and FGF had no growth-supporting effect.
Kovár
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D015237:Dinoprost / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D014284:Triiodothyronine; D013739:Testosterone; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D015237:Dinoprost; D006854:Hydrocortisone