Therapeutic application of phenylalanine immunoadsorbent with on-line regeneration.
To improve the adsorption capacity of the artificial reticuloendothelial system containing phenylalanine as a ligand, a regeneration method for the adsorbent during plasma perfusion was developed. The adsorbed rheumatoid factor, immunoglobulins, and complements were demonstrated to be elutable from the adsorbent with a 5% glucose solution in vitro. The regeneration method was applied to the treatment of a patient with rheumatoid arthritis. During each plasma perfusion, the adsorbent was regenerated, usually twice, with a 5% glucose solution at a flow rate of 50 ml/min. After each regeneration, the adsorption capacity of the adsorbent was found to be improved by determining the pre- and post-column plasma titers of a rheumatoid arthritis hemagglutination test.
Niwa
T
T
Department of Internal Medicine, Nagoya University Branch Hospital, Japan.
Nomura
T
T
Ozawa
Y
Y
Maeda
K
K
Shibata
M
M
Yamada
K
K
Oda
O
O
D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000628:therapy*; D001798:Blood Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D012157:Mononuclear Phagocyte System / Q000187:drug effects*; D010649:Phenylalanine / Q000627:therapeutic use*; D012217:Rheumatoid Factor / Q000032:analysis
D001798:Blood Proteins; D010649:Phenylalanine; D012217:Rheumatoid Factor