Fatty acid specificity of the lysosomal acid cholesterol esterase in intact human arterial smooth muscle cells.
The fatty-acid specificity of the lysosomal cholesterol esterase was examined in cultured human arterial smooth muscle cells. The lysosomal compartment of cultured cells was enriched with cholesteryl esters by incubation of cells with 0.2 mg/ml low-density lipoprotein and 50 microM chloroquine for 24 h. The hydrolysis of cholesteryl esters was subsequently induced by incubating cells in a medium containing 5% lipoprotein-deficient serum without chloroquine. Cellular cholesteryl ester mass was markedly reduced after 23 h in the lipoprotein-deficient serum. Fatty-acid analysis of cholesteryl esters in cells before and after the 23 h incubation with lipoprotein-deficient serum revealed that polyunsaturated cholesteryl esters (linoleate and arachidonate) were preferentially hydrolyzed compared to cholesteryl oleate or saturated cholesteryl esters. An increase in the ratio of cholesteryl oleate to cholesteryl linoleate was observed even when the cellular activity of acyl-CoA:cholesterol acyltransferase was inhibited with Sandoz Compound 58-035. We conclude that, in human arterial smooth muscle cells, the lysosomal acid cholesterol esterase preferentially hydrolyzes polyunsaturated cholesteryl esters.
Slotte
J P
JP
Department of Medicine, University of Washington, Seattle.
Bierman
E L
EL
D001158:Arteries / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002478:Cells, Cultured; D002788:Cholesterol Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D008247:Lysosomes / Q000201:enzymology; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D002787:Sterol Esterase / Q000032:analysis*; D013379:Substrate Specificity
D002788:Cholesterol Esters; D005227:Fatty Acids; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase