Dot-immunobinding assay as an accurate and versatile technique for the quantification of human IgG subclasses.
A dot-immunobinding assay on nitrocellulose membranes has been developed for the quantification of human IgG subclasses using subclass-specific monoclonal antibodies. The advantages of this technique can be summarized as follows: (1) possibility of rapid semi-quantitative evaluation and/or precise quantitation from the same dot-pattern; (2) simple procedure with very good reproducibility; (3) sensitivity for nanogram concentrations of individual subclasses, therefore applicable not only to serum but also to other body fluids with a low content of IgG; (4) very small amounts of test material needed; (5) very good correlation of results with other techniques (ELISA, radial immunodiffusion) but without some of the inherent problems of the latter methods.
Jol-Van der Zijde
C M
CM
Department of Pediatrics, University Hospital, Leiden, The Netherlands.
Labadie
J
J
Vlug
A
A
Radl
J
J
Vossen
J M
JM
Van Tol
M J
MJ
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003101:Collodion; D003720:Densitometry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification / Q000592:standards; D010209:Paper; D011863:Radioimmunoassay; D012015:Reference Standards
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D003101:Collodion