Identification of T-kinin-Leu(T-kinin-containing peptide) released from T-kininogen by cathepsin D of granulomatous tissues in rats.
Acid proteinases of granulomatous tissues in rats with carrageenin-induced inflammation released kinin from T-kininogen. By column chromatography on pepstatin-Sepharose 4B, two types of acid proteinase seems to be responsible for kinin release. One of the acid proteinase was identified as cathepsin D from SDS-polyacrylamide gel electrophoresis and Western-blot analysis, using anti-rat liver cathepsin D IgG. Cathepsin D alone could not release T-kinin, but T-kinin-containing peptides. The T-kinin-containing peptides were separated into two peptides by reverse-phase high-performance liquid chromatography. From determination of its amino acid composition and its immunoreactivity toward anti-bradykinin antiserum, one of the T-kinin-containing peptides was identified as T-kinin-Leu.
Sakamoto
W
W
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
Satoh
F
F
Nagasawa
S
S
Handa
H
H
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002351:Carrageenan; D002402:Cathepsin D / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006099:Granuloma / Q000209:etiology / Q000378:metabolism*; D007704:Kininogens / Q000378:metabolism*; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects
D000596:Amino Acids; D007704:Kininogens; C054879:bradykinin, Leu-Ile-Ser-; C039065:T-kinin; D002351:Carrageenan; D002402:Cathepsin D; D001920:Bradykinin