title: Glycoconjugates secreted by bovine tracheal serous cells in culture.
abstract: Glycoconjugates secreted by bovine tracheal gland serous cells in culture were characterized after incorporation of radioactive precursor [1-14C]glucosamine and stimulation with isoproterenol. Under dissociative conditions, glycoconjugates eluted in both the void and included volumes on Sepharose Cl-4B. Fractionated by anion-exchange chromatography, the high-molecular-weight (Sepharose Cl-4B; V0) glycoconjugates gave two acidic fractions eluting at 0.5 and 2.0 M NaCl; low-molecular-weight glycoconjugates of the included volumes gave a neutral fraction and two acidic fractions eluting at 0.5 and 2.0 M NaCl. Based on chemical analysis and specific enzymatic digestions, the material eluting in the void volume was shown to contain hyaluronic acid and chondroitin sulfate proteoglycan. In addition, the presence of small amounts of galactose, fucose, sialic acid, glucosamine, and galactosamine suggest the presence of O-glycosidically linked glycoproteins in the void volume. The identification of galactosaminitol in beta-eliminated oligosaccharides from this material confirms this notion. The material eluting in the included volume was shown to contain N-linked glycoproteins with glycans of complex type in the neutral fraction and chondroitin sulfate proteoglycans in the two acidic fractions. Significant N-sulfation of amino sugars was detected in the 0.5 M acidic fraction, indicating the presence of heparan sulfate. Hyaluronic acid and chondroitin sulfate proteoglycan have recently been identified in tracheal secretions; our results suggest that these components originate at least in part from tracheal gland serous cells.
authors.affiliation: INSERM U 181, Faculte de Medecine Saint-Antoine, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Paul
authors.forename: J
authors.initials: J
authors.lastname: Picard
authors.forename: M
authors.initials: M
authors.lastname: Mergey
authors.forename: D
authors.initials: D
authors.lastname: Veissiere
authors.forename: W E
authors.initials: WE
authors.lastname: Finkbeiner
authors.forename: C B
authors.initials: CB
authors.lastname: Basbaum
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000302:isolation & purification; D002417:Cattle; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005088:Exocrine Glands / Q000166:cytology / Q000378:metabolism*; D006001:Glycoconjugates / Q000302:isolation & purification / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D009092:Mucous Membrane / Q000166:cytology / Q000378:metabolism; D011509:Proteoglycans / Q000302:isolation & purification; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D006001:Glycoconjugates; D011509:Proteoglycans; D007545:Isoproterenol