Immunochemical characterisation of the myofibrillar proteinase from cultured rat myocytes as chymase.
The proteinase extracted from the myofibrillar fraction of (a) primary rat myocytes and (b) the L-8 myogenic cell line, both maintained in culture, was identified by immunochemical analysis as chymase, the chymotrypsin-like serine proteinase of rat mast cells. Chymase would therefore appear to be an intrinsic protein in the rat myocyte also.
Heath
R
R
Dept. of Biochemistry, University College, Cardiff, Wales, U.K.
Kay
J
J
Kuehn
L
L
Dahlmann
B
B
Stauber
W T
WT
Mayer
M
M
D000818:Animals; D002478:Cells, Cultured; D053818:Chymases; D003429:Cross Reactions; D010450:Endopeptidases / Q000145:classification* / Q000276:immunology; D008407:Mast Cells / Q000201:enzymology; D009132:Muscles / Q000201:enzymology*; D051381:Rats; D012697:Serine Endopeptidases / Q000145:classification* / Q000276:immunology
D010450:Endopeptidases; D012697:Serine Endopeptidases; D053818:Chymases