title: Immunohistochemical localization of chondroitin sulphate and dermatan sulphate proteoglycans in tumour tissues.
abstract: Immunohistochemical localization of chondroitin sulphate and dermatan sulphate proteoglycans (PGs) was observed in 70 tumour tissues, using monoclonal antibodies 9A-2 and 3B-3 raised against core molecules obtained from chondroitin sulphate PG by chondroitinase ABC-treatment. They recognize a stub of delta Di-4S and delta Di-6S binding to core protein via a linkage tetrasaccharide, respectively. The antibody 6B6 raised against dermatan sulphate PG obtained from an ovarian fibroma capsule in our laboratory was also used. The interstitial fibrous elements, so-called 'specific stroma' within the cancer cell nests contained chondroitin 4-sulphate PG as revealed with 9A-2, whereas the surrounding connective tissue and the preexisting fibrous connective tissue involved in the tumour growth consisted of dermatan sulphate PG with a considerable amount of chondroitin 4-sulphate PG. Chondroitin 6-sulphate PG as revealed with 3B-3 was located in the connective tissue proliferating from blood vessels and muscle tissue in association with the invasive growth of tumour cells. Chondroitin 6-sulphate PG was also observed in the basement membrane components of some tumours. In non-epithelial tumours (fibrogenic, chondrogenic, osteogenic and neurogenic tumours), chondroitin 4-sulphate was in fibrous portions. When collagenization and hyalinization progressed, dermatan sulphate PG was observed to increase in quantity.
authors.affiliation: Division of Pathology, Nagoya University Hospital, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukatsu
authors.forename: M
authors.initials: M
authors.lastname: Sobue
authors.forename: T
authors.initials: T
authors.lastname: Nagasaka
authors.forename: N
authors.initials: N
authors.lastname: Ohiwa
authors.forename: S
authors.initials: S
authors.lastname: Fukata
authors.forename: N
authors.initials: N
authors.lastname: Nakashima
authors.forename: J
authors.initials: J
authors.lastname: Takeuchi
mesh_terms: D053674:Aggrecans; D000911:Antibodies, Monoclonal / Q000276:immunology; D002807:Chondroitin / Q000031:analogs & derivatives*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D003238:Connective Tissue / Q000378:metabolism; D003871:Dermatan Sulfate / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*
chemical_list: D053674:Aggrecans; D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D009363:Neoplasm Proteins; D011509:Proteoglycans; C032649:dermatan sulfate proteoglycan; D003871:Dermatan Sulfate; D002807:Chondroitin