Production of monoclonal antibodies against phloem-limited prokaryotes of plants: a general procedure using extracts from infected periwinkles as immunogen.
Using Spiroplasma citri-infected periwinkle extracts as the source of antigens, together with monoclonal antibody technology, we determined the conditions for mouse immunization and developed a screening assay to detect those hybridomas which produce immunoglobulins specifically directed against the pathogen.
Martin-Gros
G
G
Laboratoire de Biologie cellulaire et moléculaire, INRA, Pont-de-la-Maye, France.
Iskra
M L
ML
Garnier
M
M
Gandar
J
J
Bové
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000941:Antigens / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010935:Plant Diseases; D010936:Plant Extracts / Q000276:immunology*; D013149:Spiroplasma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D000942:Antigens, Bacterial; D010936:Plant Extracts