title: Field observations on the use of anti-sporozoite monoclonal antibodies for determination of infection rates in malaria vectors.
abstract: Samples of indoor-resting Anopheles gambiae s.1. from Mali and Burkina Faso (West Africa) were processed in order to compare Plasmodium falciparum sporozoite rates obtained by immunoradiometric assay (IRMA) with circumsporozoite (CS) monoclonal antibody and by microscope examination of salivary glands. The immunological method provided sporozoite rates always higher than those obtained by microscope examination. This result does not appear to be related to cross-reactions involving non-sporozoite antigens. A small fraction of IRMA-positive mosquitoes is necessarily negative by microscope, since these mosquitoes actually contain the CS antigen only in the abdomen, presumably in connection with the presence of fully mature oocysts. However, the frequency of these mosquitoes cannot explain in itself an average ratio of 1:2 between microscope and IRMA sporozoite rates. A more important source of difference appears to depend on the detection of positive mosquitoes with low sporozoite numbers which remain more frequently undetected by microscope examination. Failure of salivary gland penetration by sporozoites is also considered as a possible source of discrepancy between the two methods.
authors.affiliation: Dipartimento di Biologia Cellulare, Università di Camerino, Italia.
authors.forename: F
authors.initials: F
authors.lastname: Esposito
authors.forename: S
authors.initials: S
authors.lastname: Lombardi
authors.forename: Y T
authors.initials: YT
authors.lastname: Toure
authors.forename: F
authors.initials: F
authors.lastname: Zavala
authors.forename: M
authors.initials: M
authors.lastname: Coluzzi
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D007163:Immunosorbent Techniques; D007303:Insect Vectors / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal