title: Hydrogen-mediated mannose uptake in Azotobacter vinelandii.
abstract: Azotobacter vinelandii can grow mixotrophically with H2 plus mannose under N2-fixing conditions (T. Y. Wong and R. J. Maier, J. Bacteriol. 163:528-533, 1985). Mixotrophically grown cultures incubated in H2 transported mannose with a Vmax fourfold greater than that observed for cultures incubated in argon, but H2 did not change the apparent Km for mannose. Respiratory inhibitors, such as potassium cyanide, hydroxylamine, and p-chloromercuribenzoic acid, as well as the proton conductor carbonyl cyanide m-chlorophenyl-hydrazone inhibited mannose uptake. We suggest that one of the roles of H2 in mixotrophic metabolism is to supply energy that facilitates mannose transport.
authors.affiliation: Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
authors.forename: R J
authors.initials: RJ
authors.lastname: Maier
authors.forename: J
authors.initials: J
authors.lastname: Prosser
mesh_terms: D001395:Azotobacter / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002729:Chloromercuribenzoates / Q000494:pharmacology; D006859:Hydrogen / Q000378:metabolism*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism*; D011190:Potassium Cyanide / Q000494:pharmacology; D020245:p-Chloromercuribenzoic Acid
chemical_list: D002729:Chloromercuribenzoates; D006898:Hydroxylamines; D019811:Hydroxylamine; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D020245:p-Chloromercuribenzoic Acid; D006859:Hydrogen; D011190:Potassium Cyanide; D008358:Mannose