title: The disulfide bridges of the immunoreactive forms of human pancreatic stone protein isolated from pancreatic juice.
abstract: Following the complete sequence elucidation of human pancreatic stone protein (immunoreactive form PSP S1 isolated from pancreatic juice) [(1987) Eur. J. Biochem. 168, 201-207], the location of the three S-S bridges of the protein was investigated. The cystine-containing peptides, detected after the separation of the peptic or chymotryptic digests on SP-Sephadex or Sephadex G-50, were submitted to Edman degradation and/or to oxidation. The cysteic peptides after separation on SP-Sephadex or Sephadex G-50 were characterized by their amino acid compositions. The pairing of the half-cystines: Cys 3-Cys 14, Cys 31-Cys 129 and Cys 104-Cys 121 was determined. The same experiments carried out with PSP S2-5 (other immunoreactive forms) gave an identical characterization.
authors.affiliation: Centre de Biochimie et de Biologie Moléculaire, CNRS, Marseille, France.
authors.forename: P
authors.initials: P
authors.lastname: Rouimi
authors.forename: J
authors.initials: J
authors.lastname: de Caro
authors.forename: J
authors.initials: J
authors.lastname: Bonicel
authors.forename: M
authors.initials: M
authors.lastname: Rovery
authors.forename: A
authors.initials: A
authors.lastname: de Caro
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis*; D004220:Disulfides / Q000032:analysis*; D006801:Humans; D051847:Lithostathine; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010084:Oxidation-Reduction; D010189:Pancreatic Juice / Q000032:analysis*
chemical_list: D000596:Amino Acids; D002135:Calcium-Binding Proteins; D004220:Disulfides; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human