Qa-2 antigen encoded by Q7b is biochemically indistinguishable from Qa-2 expressed on the surface of C57BL/10 mouse spleen cells.
Qa-2 was immunoprecipitated from the surface of 125I-labeled C57BL/10 (B10) mouse spleen cells and compared with Qa-2 immunoprecipitated from the surface of R1.1 thymoma cells transfected with Q7b. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that Qa-2 glycoproteins from both of these sources have a relative molecular mass of approximately 37 kDa. After treatment with endoglycosidase F, the Qa-2 polypeptide chains derived from C57BL/10 spleen and Q7b-transfected R1.1 cells displayed identical mobilities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis because of removal of N-linked oligosaccharide residues. Furthermore, treatment of Qa-2 proteins from both sources with cyanogen bromide or alpha-chymotrypsin resulted in identical peptide fragmentation patterns. These results therefore provide a biochemical correlation between a cloned Qa-region gene produce expressed on the surface of transfected cells, and the Qa-2 glycoprotein on spleen cells that was described a decade ago by serologic methods.
Sherman
D H
DH
Biogen Research Corp., Cambridge, MA 02142.
Waneck
G L
GL
Flavell
R A
RA
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D002460:Cell Line; D005796:Genes; D005805:Genes, MHC Class I; D006023:Glycoproteins / Q000235:genetics; D006649:Histocompatibility Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D013154:Spleen / Q000276:immunology; D014162:Transfection
D000954:Antigens, Surface; D006023:Glycoproteins; D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments; C032907:Q surface antigens