Evaluation of monoclonality of cell lines from sequential dilution assays. Part II.
We extend the analysis in Koziol et al. (J. Immunol. Methods (1987) 105, 139) for the determination of the probability of achieving monoclonality in limiting dilution assays to three or more cloning stages. We also provide a computer program which will carry out this analysis for an arbitrary number of stages.
Koziol
J A
JA
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, CA 92037.
D000911:Antibodies, Monoclonal; D002460:Cell Line; D006825:Hybridomas / Q000166:cytology*; D011336:Probability; D012984:Software
D000911:Antibodies, Monoclonal