Nanophthalmic sclera. Fibronectin studies.
The authors performed fibronectin studies on scleral specimens derived from a patient with nanophthalmos. Immunohistochemical staining with antifibronectin was conducted using both formaldehyde-fixed, paraffin-embedded tissue sections and unfixed tissue-cultured scleral cells. In each case, the nanophthalmic samples exhibited fibronectin staining stronger than that obtained from normal human subjects. Results from an enzyme-linked immunosorbent assay (ELISA) confirmed the histologic findings that, in tissue culture, the patient's scleral cells contained and secreted a higher amount of fibronectin than did the normal control cells. The elevated fibronectin level may be related to the development of nanophthalmos.
Yue
B Y
BY
Department of Ophthalmology, University of Illinois at Chicago, Lions of Illinois Eye Research Institute 60612.
Kurosawa
A
A
Duvall
J
J
Goldberg
M F
MF
Tso
M O
MO
Sugar
J
J
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005128:Eye Diseases / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D012590:Sclera / Q000378:metabolism / Q000473:pathology
D005353:Fibronectins