Isolation and genetic analysis of operator-constitutive mutants of the H1 operon in Salmonella typhimurium.
In phase-2 cells of diphasic Salmonella strains, expression of H1 is repressor, coded for by the rh1 gene. A procedure for the isolation of operator-constitutive (H1-Oc) mutants of the H1 operon is described. Using three-factor crosses between an H1-Oc H1 strain and H1-O+ H1 strains, where motility recovery via H1-phase (or phase 1) flagellation was used as the selected marker and the H1-O character was the unselected marker, the relative position of the H1-Oc site to the H1 gene was determined. A diphasic H1-Oc strain produced, in phase 2, copolymer filaments composed of H1 and H2 flagellin.
Fujita
H
H
Department of Biology, School of Education, Waseda University, Tokyo, Japan.
Yamaguchi
S
S
Taira
T
T
Hirano
T
T
Iino
T
T
D005407:Flagella / Q000648:ultrastructure; D005798:Genes, Bacterial; D008854:Microscopy, Electron; D009154:Mutation; D009876:Operon; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic