title: The primary structure of human platelet profilin: reinvestigation of the calf spleen profilin sequence.
abstract: The primary structure of human platelet profilin was determined by aligning the sequences of its tryptic peptides to the previously determined calf spleen profilin sequence [(1979) FEBS Lett. 101, 161-165]. Comparison of the peptide fingerprints of the two proteins suggested a higher homology than that found by direct sequence comparison. We therefore reinvestigated the sequences of the peptides from calf spleen profilin. We identified four incorrect charge assignments and a deletion of three residues. The similarity between the two vertebrate profilins amounts to 95%.
authors.affiliation: Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Ampe
authors.forename: F
authors.initials: F
authors.lastname: Markey
authors.forename: U
authors.initials: U
authors.lastname: Lindberg
authors.forename: J
authors.initials: J
authors.lastname: Vandekerckhove
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D002417:Cattle; D003285:Contractile Proteins; D004590:Electrophoresis, Paper; D006801:Humans; D008840:Microfilament Proteins / Q000032:analysis; D051304:Profilins; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000032:analysis*
chemical_list: D003285:Contractile Proteins; D008840:Microfilament Proteins; C495738:PFN1 protein, human; D051304:Profilins