Separation and quantitative analysis of some carotenoid fatty acid esters of fruits by liquid chromatography.
The adsorption and partition properties of several fatty acid esters of capsanthin, capsorubin, beta-cryptoxanthin, lutein, violaxanthin and beta-citraurin isolated from fruits were studied by normal-phase (silica) and reversed-phase (octadecylsilane) liquid chromatography using Sudan 1 as internal standard. The separation on a normal phase was based on the functional group of the carotenoids and individual esters of the same carotenoid did not resolve. The separation on a reversed phase was more dependent on the number of acyl carbons than the functional group, and individual esters of the same carotenoid differing only two acyl carbons were separated with a resolution of 3. There was a linear relationship between number of acyl carbons and retention times of the same carotenoid on reversed phase. The separation on a normal phase was the reverse of that on a reversed phase, and a combination of normal-phase followed by reversed-phase chromatography was used for the separation of esters with the same or close retention times.
Philip
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Department of Home Economics, California State University, Northridge 91330.
Chen
T S
TS
D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis*; D005638:Fruit / Q000032:analysis*; D007202:Indicators and Reagents
D005227:Fatty Acids; D007202:Indicators and Reagents; D002338:Carotenoids