Analysis of the Klebsiella pneumoniae ntrB gene by site-directed in vitro mutagenesis.
A number of in-frame insertion and deletion mutations have been constructed in vitro in the Klebsiella pneumoniae ntrB gene and the effects of each mutant NtrB protein on NtrC activity have been assessed after reintroduction of the ntrB mutation into the glnA ntrBC operon. These experiments suggest that the phosphorylation of NtrC catalysed by NtrB not only makes NtrC competent as a transcriptional activator but also improves the DNA-binding properties and hence the negative control functions of NtrC. The variety of NtrB phenotypes obtained suggest a structure/function model for the protein.
MacFarlane
S A
SA
AFRC Unit of Nitrogen Fixation, University of Sussex, Brighton, UK.
Merrick
M J
MJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D005796:Genes; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010749:Phosphoprotein Phosphatases; D010957:Plasmids; D011494:Protein Kinases; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D011494:Protein Kinases; C055699:protein kinase-phosphatase NTRB; D010749:Phosphoprotein Phosphatases; D009584:Nitrogen