Quantitation of indirect sandwich enzyme-linked immunosorbent assay parameters.
The optimization of data from the indirect sandwich enzyme-linked immunosorbent assay has been commonly accomplished by linear regression analysis, even though the data are often essentially sigmoid. A new microcomputer software program (LISACRV) that uses a nonlinear regression statistical model to analyze the data from enzyme-linked immunosorbent assay titration experiments was developed.
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Division of Agriculture, Arizona State University, Tempe 85287.
D004797:Enzyme-Linked Immunosorbent Assay; D008838:Microcomputers; D012984:Software