title: A model for the hapten-induced enhancement of monoclonal antibody binding in ELISA.
abstract: The unusual binding properties of three anti-phosphocholine (PC) monoclonal antibodies (MAbs) in ELISA has been reported [Hall T. J. (1987) Molec. Immun. 24, 773-777]. These MAbs gave reproducible 2-4 fold increases in binding to PC-protein coated plates in the presence of PC (approximately 2 mM), nitrophenyl PC (approximately 10 micron) and other PC analogues. A model is proposed here in which the complementary and opposite binding of hapten and PC-protein in the combining sites of these MAbs explains the hapten enhanceable binding in ELISA.
authors.affiliation: Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201.
authors.forename: T J
authors.initials: TJ
authors.lastname: Hall
authors.forename: C Y
authors.initials: CY
authors.lastname: Kang
mesh_terms: D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens; D008956:Models, Chemical; D010767:Phosphorylcholine
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D010767:Phosphorylcholine