Control and isotopic quantification of affinity of antithrombin III for heparin-like surfaces.
Heparin-like materials, characterized by a defined superficial density of functional groups which activate antithrombin III (AT III), when in contact with blood specifically inhibit thrombin as soon as it appears. This paper describes an isotopic method to estimate this density and to visualize the distribution of the affinity sites concerned, both directly with AT III labelled with 125Iodine and indirectly with an anti AT III monoclonal antibody labelled with 111Indium.
Caix
J
J
CNRS-GRECO no. 48 Polymères Hémocompatibles, Universitè de Bordeaux II, France.
Migonney
V
V
Baquey
A
A
Fougnot
C
C
Perrot Minnot
A
A
Beziade
A
A
Vuillemin
L
L
Baquey
C
C
Ducassou
D
D
D000327:Adsorption; D000911:Antibodies, Monoclonal; D000990:Antithrombin III; D001672:Biocompatible Materials; D006493:Heparin; D007118:Immunoassay; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D011137:Polystyrenes
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D011137:Polystyrenes; D000990:Antithrombin III; D006493:Heparin