title: [Submerged-phase gel electrophoresis in the analysis of plasmids for epidemiologic purposes].
abstract: The validity of the submarine agarose gel electrophoresis as routine method for plasmidic epidemiology was considered. Using standard plasmids, the efficiency of a performed protocol conditions (5 V/cm for 3 hours, 0.8% agarose) in screening plasmids of different size was studied. The results provide sharp profile resolution and an accurate estimate of molecular weights in the range from 1.2 to 112 megadaltons.
authors.affiliation: Dipartimento di Igiene Università, Perugia.
authors.forename: G
authors.initials: G
authors.lastname: Caldini
authors.forename: G
authors.initials: G
authors.lastname: Cenci
authors.forename: G
authors.initials: G
authors.lastname: Morozzi
mesh_terms: D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005069:Evaluation Studies as Topic; D008970:Molecular Weight; D010957:Plasmids; D012015:Reference Standards
vernacular_title: Gel elettroforesi in fase sommersa nell'analisi plasmidica a fini epidemiologici.