Identification of polypeptides of the carbon metabolism machinery on the two-dimensional protein map of Saccharomyces cerevisiae. Location of 23 additional polypeptides.
Using a modification of the basic two-dimensional polyacrylamide gel electrophoresis technique, we have undertaken a systematic identification of the polypeptides of the protein map of Saccharomyces cerevisiae corresponding to components of the carbon metabolism machinery. To the previous location of nine glycolytic enzyme polypeptides on the yeast protein map we add the location of 23 polypeptides. Ten of them were identified as corresponding to cytoplasmic enzymes of the carbon metabolism machinery and 13 were characterized as mitochondrial proteins. The criteria used to establish the identification of these polypeptides spots include migration with purified proteins, immunodetection, overproduction by plasmid-carrying strains and physiological behaviour.
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Laboratoire de Génétique, Allée des Facultés, Facultés des Sciences, Talence, France.
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D002244:Carbon / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis*; D008928:Mitochondria / Q000032:analysis; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D005656:Fungal Proteins; D010455:Peptides; D002244:Carbon