title: The solution structure of concanavalin A probed by FT-IR spectroscopy.
abstract: The secondary structural properties of various forms of concanavalin A in solution were investigated by Fourier-transform infrared spectroscopy in the Amide I region. As in the crystal, the solution structure of the native protein consists mainly of antiparallel beta-sheet. Carbohydrate binding does not produce major changes in the overall secondary structure of concanavalin A, but affects infrared bands due to loops and beta-turns. Upon demetallization, the spectrum of concanavalin A shows only a small change in the Amide I band, indicating that whereas the beta-sheet structure is conserved, the tertiary properties may be altered. There are also changes in the bands from the tyrosine residues which are compatible with local changes in structure. Confirming tertiary structural differences, the cation-depleted apoprotein is much less stable, denaturing around 63 degrees C, while the native protein denatures only at temperatures around 85 degrees C. Tetramerization proceeds without significant secondary structural change. However, aggregation of the tetramers leads to a significant decrease of the bands corresponding to beta-sheet structure, and changes in the tyrosine bands.
authors.affiliation: Department of Biochemistry, University of the Basque Country, Bilbao, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Arrondo
authors.forename: N M
authors.initials: NM
authors.lastname: Young
authors.forename: H H
authors.initials: HH
authors.lastname: Mantsch
mesh_terms: D002118:Calcium / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003208:Concanavalin A / Q000032:analysis; D005583:Fourier Analysis; D046911:Macromolecular Substances; D008345:Manganese / Q000378:metabolism; D011487:Protein Conformation; D011952:Receptors, Concanavalin A / Q000032:analysis; D012996:Solutions; D013055:Spectrophotometry, Infrared; D014443:Tyrosine / Q000032:analysis
chemical_list: D046911:Macromolecular Substances; D011952:Receptors, Concanavalin A; D012996:Solutions; D003208:Concanavalin A; D014443:Tyrosine; D008345:Manganese; D002118:Calcium