Immunological detection of high molecular weight proteins by gel and blot overlay.
The sensitivity and specificity of the gel overlay and western blot methods of immunodetection are compared for spectrins, typical high molecular weight proteins. The gel overlay method is more sensitive and specific for the immunodetection of brain spectrin (240/235) and rbc spectrin. As the western blot technique will remain the method of choice for many applications because of its relative speed, we discuss methods for optimizing its sensitivity and selectivity.
Riederer
B M
BM
Department of Physiology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Goodman
S R
SR
D000818:Animals; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis; D007158:Immunologic Techniques; D051379:Mice; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis; D013049:Spectrin / Q000032:analysis*
D009419:Nerve Tissue Proteins; D013049:Spectrin