title: Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles.
abstract: DNA enriched for supercoiled plasmids prepared from the 3 micron plasmid-enriched, [psi+], [2 micron degrees] strain 6-1G-P188 and from the [2 micron+] [psi+] strain LL20 can be used to transform a psi- recipient strain to psi+. Fractionation of the former preparation by electrophoresis showed that the 3 micron plasmid band contained the transforming activity.
authors.affiliation: Institute of Botany, Academia Sinica, Taipei, Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Dai
authors.forename: S H
authors.initials: SH
authors.lastname: Tsay
authors.forename: P M
authors.initials: PM
authors.lastname: Lund
authors.forename: B S
authors.initials: BS
authors.lastname: Cox
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D004278:DNA, Superhelical / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D004278:DNA, Superhelical