Felodipine-induced inhibition of polymorphonuclear leukocyte functions.
Felodipine inhibits fMet-Leu-Phe or ionophore A23187-induced exocytosis in rabbit peritoneal polymorphonuclear leukocytes (PMNs), in the concentration range 1-50 microM. Activation of the metabolic burst, and migration of PMNs towards fMet-Leu-Phe are equally inhibited by felodipine in the same concentration range. The effect is not due to blocking of calcium channels in the plasma membrane, because the degree of inhibition remains the same when Ca2+ is omitted from the medium. Felodipine interferes with ionophore A23187-induced association of 45Ca with the PMN but this interference occurs at lower concentrations than the inhibition of exocytosis. Hypotonic hemolysis of erythrocytes is inhibited by felodipine; maximal protection against hemolysis occurs at a concentration of 50 microM felodipine. It is suggested that at least a part of the inhibiting effect on PMN functions might be due to an anesthetic-like membrane effect of felodipine.
Elferink
J G
JG
Department of Medical Biochemistry, University of Leiden, Sylvius Laboratories, The Netherlands.
Deierkauf
M
M
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004912:Erythrocytes / Q000502:physiology; D005089:Exocytosis / Q000187:drug effects; D015736:Felodipine; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009568:Nitrendipine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009580:Nitroblue Tetrazolium; D011299:Prenylamine / Q000494:pharmacology; D011817:Rabbits
D002121:Calcium Channel Blockers; D009580:Nitroblue Tetrazolium; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009568:Nitrendipine; D007770:L-Lactate Dehydrogenase; D009113:Muramidase; D011299:Prenylamine; D015736:Felodipine; D002118:Calcium