Chicken antibodies: a tool to avoid false positive results by rheumatoid factor in latex fixation tests.
19 rheumatoid factor (RF)-positive sera (Waaler-Rose test) and ten control sera were tested in latex agglutination. All RF-positive sera agglutinated latex particles coated with the different mammalian IgGs (cow, horse, human, mouse, sheep and rabbit) and with chicken anti-human C3 (which was used as a positive control), but none of them with non-specific chicken IgG. The control sera were all negative except when tested with chicken anti-human C3. Chicken antibodies should therefore be useful in assays were RF could interfere and give false positive reactions (e.g., nephelometry, latex agglutination or ELISA).
Larsson
A
A
Department of Medical and Physiological Chemistry, Biomedical Centre, Uppsala, Sweden.
Sjöquist
J
J
D000818:Animals; D002417:Cattle; D002645:Chickens / Q000276:immunology*; D003176:Complement C3 / Q000276:immunology; D003429:Cross Reactions; D005189:False Positive Reactions; D006736:Horses; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007841:Latex Fixation Tests / Q000379:methods*; D051379:Mice; D011817:Rabbits; D012217:Rheumatoid Factor / Q000276:immunology*; D012756:Sheep; D013045:Species Specificity
D003176:Complement C3; D007074:Immunoglobulin G; D012217:Rheumatoid Factor