Novobiocin induces positive supercoiling of small plasmids from halophilic archaebacteria in vivo.
The halophilic archaebacterium Halobacterium strain GRB harbours a multicopy plasmid of 1.7 kb which is negatively supercoiled. After addition of novobiocin to culture medium all 1.7 kb plasmid molecules become positively supercoiled. Positive supercoiling occurs at the same dose of novobiocin inhibiting the eubacterial DNA gyrase in vitro. Novobiocin also induces positive supercoiling of pHV2, a 6.3 kb plasmid from Halobacterium volcanii. These results indicate the existence of a mechanism producing positive superturns in halobacteria. The 1.7 kb plasmid from Halobacterium GRB could be used to produce high amounts of pure positively supercoiled DNA for biophysical and biochemical studies.
Sioud
M
M
Laboratoire de Biologie Moléculaire de la Réplication ER 272, CNRS, Villejuif, France.
Baldacci
G
G
de Recondo
A M
AM
Forterre
P
P
D004278:DNA, Superhelical / Q000187:drug effects; D006217:Halobacterium / Q000235:genetics*; D009675:Novobiocin / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D009994:Osmolar Concentration; D010957:Plasmids / Q000187:drug effects*
D004278:DNA, Superhelical; D009675:Novobiocin