Paper-disc method for Campylobacter hippurate-hydrolysis test. A note.
Paper discs were impregnated with sodium hippurate dissolved in 1/15 phosphate buffer pH 7.2. The discs, each containing 2 mg substrate, were stored in dry state at room temperature. After adding a disc to bacteria suspended in distilled water, and incubated at 37 degrees C for 2 h, one drop of Skirrow and Benjamin's ninhydrin reagent was added and the reaction was read after re-incubation at 37 degrees C for 10 min. 169 campylobacter strains were tested. The results for a total of 169 strains were identical with those obtained with the modified rapid method of Skirrow and Benjamin. Of 92 campylobacter strains isolated from human faecal samples 59.8%, of 36 strains isolated from slaughtered poultry 41.7% proved to be Campylobacter jejuni.
Kotsis
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IH
National Institute of Hygiene, Budapest, Hungary.
Adám
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M
D000818:Animals; D001431:Bacteriological Techniques; D002168:Campylobacter fetus / Q000145:classification / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D006626:Hippurates; D006801:Humans; D006868:Hydrolysis; D012703:Serotyping
D006626:Hippurates; C030514:hippuric acid