Purification of the LSTRA tyrosine protein kinase (p56lck).
p56lck, a p60src related tyrosine protein kinase was recently described in a murine lymphoma cell line (LSTRA), in several human lymphomas and in normal peripheral lymphocytes. We have purified p56lck to homogeneity by electrofocusing followed by two-step SDS/polyacrylamide gel electrophoresis. The pure protein was identical to the p56 characterized in LSTRA cells and used to raise antibodies in rabbits. This simple procedure is applicable for the rapid purification of minor proteins.
Van Tan
H
H
INSERM U 15, Institut de Pathologie Moléculaire, Paris, France.
Fischer
S
S
Fagard
R
R
D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007942:Leukemia, Experimental / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008223:Lymphoma / Q000201:enzymology; D051379:Mice; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000302:isolation & purification*
D000596:Amino Acids; D011505:Protein-Tyrosine Kinases