[Electrophysiology and analysis of the image of the development of Purkinje cells in culture and in the intact animal].
The electrophysiological and morphological properties of the Purkinje cells (P cells) grown in organotypic cultures were studied in HRP intracellulary labelled neurons and compared to Golgi stained P cells from kitten of different ages. The effects of afferent fiber depletions on the final dendritic topological parameters were investigated by 3-dimensional reconstructions and computerized methods. In culture, the P cell dendritic trees always appeared reduced in size when compared to their in vivo counterparts. A discriminant analysis permitted the recognition of 3 populations of cultured P cells according to the type of deafferentation. In intact animals, the dendritic organization appeared biplanar around day 13. These results demonstrate that the presence of all normal inputs is required to achieve the full elaboration and the monoplanar disposition of the P cell dendrites.
Calvet
M C
MC
INSERM U 249, CNRS LP8402, EPHE, Institut de Biologie, Montpellier, France.
Calvet
J
J
D000375:Aging / Q000502:physiology; D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D004594:Electrophysiology; D006056:Golgi Apparatus / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D008746:Methylazoxymethanol Acetate; D011689:Purkinje Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D013238:Stereotaxic Techniques
D008746:Methylazoxymethanol Acetate
Electrophysiologie et analyse d'image du développement des cellules de Purkinje en culture et sur l'animal entier.