title: Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation.
abstract: A monoclonal antibody of the IgM class, reacting with the CD9 (p24) antigen is described. The antibody (FMC27) is cytotoxic against cells of the common type of acute lymphoblastic leukaemia (c-ALL), giving killing at higher dilutions than an IgG antibody (FMC8) against the same antigen. FMC27 and FMC8 recognise different epitopes, and FMC27 may thus be used in a cocktail together with FMC8 and an antibody against the c-ALL antigen, WM21. Furthermore, the IgM antibody can be coated directly onto magnetic microparticles for magnetic purging, unlike the IgG antibody which must be used in a two-layer procedure.
authors.affiliation: Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, Australia.
authors.forename: H
authors.initials: H
authors.lastname: Zola
authors.forename: A
authors.initials: A
authors.lastname: Potter
authors.forename: S H
authors.initials: SH
authors.lastname: Neoh
authors.forename: C A
authors.initials: CA
authors.lastname: Juttner
authors.forename: D N
authors.initials: DN
authors.lastname: Haylock
authors.forename: A M
authors.initials: AM
authors.lastname: Rice
authors.forename: E J
authors.initials: EJ
authors.lastname: Favaloro
authors.forename: A
authors.initials: A
authors.lastname: Kabral
authors.forename: K F
authors.initials: KF
authors.lastname: Bradstock
mesh_terms: D000911:Antibodies, Monoclonal / Q000627:therapeutic use* / Q000633:toxicity; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000276:immunology; D000961:Antilymphocyte Serum / Q000633:toxicity; D001853:Bone Marrow / Q000276:immunology*; D016026:Bone Marrow Transplantation; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000627:therapeutic use*; D015470:Leukemia, Myeloid, Acute / Q000276:immunology / Q000473:pathology / Q000628:therapy; D008212:Lymphocyte Depletion; D008562:Membrane Glycoproteins; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D060245:Tetraspanin 29; D014182:Transplantation, Autologous / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000961:Antilymphocyte Serum; C554869:CD9 protein, human; D007075:Immunoglobulin M; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29