A chicken embryo-specific myosin light chain (L23) appears to be identical with one of brain myosin light chains.
A novel embryo-specific myosin light chain of 23 kDa molecular weight (L23) was found previously in embryonic chicken skeletal, cardiac, and smooth muscles (Takano-Ohmuro et al. (1985) J. Cell Biol. 100, 2025-2030). When we examined myosin in embryonic and adult brain by two-dimensional electrophoresis, 23 kDa myosin light chain present in brain (Burridge & Bray (1975) J. Mol. Biol. 99, 1-14) comigrated with L23. Two monoclonal antibodies, EL-64 and MT-185d, were applied to clarify the identity of the brain 23 kDa myosin light chain and the chicken embryonic muscle L23. The two antibodies recognize different antigenic determinants in the L23 molecule; the former antibody is specific for L23, whereas the latter recognizes the sequence common to fast skeletal muscle myosin light chains 1 and 3, and also L23. The immunoblots combined with two-dimensional gel electrophoresis showed that both EL-64 and MT-185d can bind to the brain 23 kDa myosin light chain as well as the chicken embryonic muscle L23. These results indicate that chicken brain and chicken embryonic muscles contain a common myosin light chain of 23 kDa molecular weight.
Takano-Ohmuro
H
H
Medical Engineering Section, Tokyo Metropolitan Institute of Medical Science.
Ogasawara
Y
Y
Obinata
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry; D002642:Chick Embryo; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005895:Gizzard, Avian / Q000032:analysis / Q000196:embryology; D006651:Histocytochemistry; D007118:Immunoassay; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000032:analysis / Q000196:embryology*; D009218:Myosins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D009218:Myosins