title: Platelet-associated immunoglobulins G, A and M are secreted during platelet activation: normal levels but defective secretion in grey platelet syndrome.
abstract: Platelet-associated (PA) IgG is known to be released from normal human platelets when they are stimulated by aggregating agents. We have studied whether PA-IgA and PA-IgM are also secreted during platelet activation or during blood collection and processing and whether their levels are related to those in serum. Processing of platelets from normal donors in the presence of secretion inhibitors prostaglandin E1 (PGE1) and theophylline increased levels of both surface and total PA-immunoglobulins (Ig) in intact and lysed platelets respectively, with increases being significant for surface PA-IgA and PA-IgM and total PA-IgM. About 50% of total PA-IgM, 40% PA-IgA and 20% PA-IgG was detectable on intact platelets. All three PA-Ig and PA-albumin were secreted in response to thrombin and this release was inhibited by PGE1. The platelet:serum ratio of each Ig and albumin were similar. In grey platelets deficient in alpha-granules, PA-Ig and PA-albumin levels were raised per platelet but when increased platelet size was taken into account PA-Ig were normal and PA-albumin just below normal. Although thrombin caused release of most of the small amounts of beta-thromboglobulin present, PA-Ig and PA-albumin were not released. This suggests that PA-Ig and albumin from plasma may enter a pool of secretory proteins in normal platelets, but in grey platelets they remain in some other site.
authors.affiliation: Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria, Australia.
authors.forename: S L
authors.initials: SL
authors.lastname: Pfueller
authors.forename: R
authors.initials: R
authors.lastname: David
mesh_terms: D000527:Alprostadil / Q000494:pharmacology; D001791:Blood Platelet Disorders / Q000097:blood / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D001798:Blood Proteins / Q000378:metabolism; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D066298:In Vitro Techniques; D013577:Syndrome; D013806:Theophylline / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
chemical_list: D001798:Blood Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D013806:Theophylline; D013917:Thrombin; D000527:Alprostadil