title: Presynaptic M1 muscarinic receptor modulates spontaneous release of acetylcholine from rat basal forebrain slices.
abstract: Spontaneous release of acetylcholine (ACh) from rat basal forebrain slices in the presence of cholinesterase inhibitor was directly determined using a specific radioimmunoassay for ACh. The release was calcium dependent. A consistent amount of ACh release was observed throughout the experiment. Atropine (10(-8) to 10(-5) M) and pirenzepine (10(-7) to 10(-5) M) enhanced spontaneous ACh release. These findings indicate the presence of an M1 muscarinic autoreceptor that modulates spontaneous release of ACh in the rat basal forebrain.
authors.affiliation: Department of Pharmacology, Kyoritsu College of Pharmacy, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
authors.forename: H
authors.initials: H
authors.lastname: Oohata
authors.forename: K
authors.initials: K
authors.lastname: Kawashima
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D004027:Diencephalon / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010890:Pirenzepine / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011976:Receptors, Muscarinic / Q000502:physiology*; D013450:Sulfones / Q000494:pharmacology; D013569:Synapses / Q000502:physiology*; D013687:Telencephalon / Q000378:metabolism*
chemical_list: D011976:Receptors, Muscarinic; D013450:Sulfones; D010890:Pirenzepine; D001285:Atropine; C005109:methanesulfonyl fluoride; D000109:Acetylcholine; D002118:Calcium