A unique mRNA species for a regulatory subunit of cAMP-dependent protein kinase is specifically induced in haploid germ cells.
Cyclic AMP (cAMP) and its action by way of cAMP-dependent protein kinase is important for sperm motility. Previous studies on germ cells have demonstrated a selective decrease in the amount of type I cAMP-dependent protein kinase during spermatid development, and that type II was the major form present in elongating spermatids and in mature sperm. This would indicate activation of a gene in haploid germ cells, encoding a regulatory subunit of type II protein kinase. However, haploid expression of such a gene has so far not been shown. In the present study we demonstrate high-levelled expression of a unique mRNA species for a specific regulatory subunit of type II cAMP-dependent protein kinase at late stages of spermatogenesis, i.e. during spermatid elongation.
Oyen
O
O
Institute of Pathology, Rikshospitalet, Oslo, Norway.
Scott
J D
JD
Cadd
G G
GG
McKnight
G S
GS
Krebs
E G
EG
Hansson
V
V
Jahnsen
T
T
D000375:Aging; D000818:Animals; D006238:Haploidy; D046911:Macromolecular Substances; D008297:Male; D011494:Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013087:Spermatids / Q000201:enzymology; D013090:Spermatocytes / Q000201:enzymology; D013091:Spermatogenesis; D013737:Testis / Q000201:enzymology / Q000254:growth & development*
D046911:Macromolecular Substances; D012333:RNA, Messenger; D011494:Protein Kinases