Genetic control of NADH dehydrogenase-1 and aromatic alcohol dehydrogenase-2 in hexaploid wheat.
The genetic control of NADH dehydrogenase-1 (NDH-1) and aromatic alcohol dehydrogenase-2 (AADH-2) was investigated in Triticum aestivum cv. Chinese Spring. Evidence was obtained that NDH-1 is active as a monomer and is encoded by genes located in the p arms of the homoeologous group 4 chromosomes. The NDH-1 gene loci located in 4Ap, 4Bp, and 4Dp were designated Ndh-A1, Ndh-B1, and Ndh-D1, respectively. Aadh-A2 was previously reported to be located in 6Aq; in this study, Aadh-B2 and Aadh-D2 were localized in 6Bq and 6Dq, respectively. Alcohol dehydrogenase-1 is expressed on AADH-2 zymograms; the presence of a contaminating aliphatic alcohol in one or more reagents is suggested as the probable cause of this phenomenon.
Hart
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GE
Soil and Crop Sciences Department, Texas A&M University, College Station 77843.
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D003579:Cytochrome Reductases / Q000235:genetics*; D005796:Genes; D007527:Isoenzymes / Q000235:genetics*; D009245:NADH Dehydrogenase / Q000235:genetics*; D010641:Phenotype; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011123:Polyploidy; D014908:Triticum / Q000201:enzymology / Q000235:genetics
D007527:Isoenzymes; D000429:Alcohol Oxidoreductases; C055882:aryl-alcohol dehydrogenase; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase