Measurement of urinary lactic, 3-hydroxybutyric, pyruvic and acetoacetic acids in a single analysis using selected ion monitoring and stable isotope labelling techniques.
Sodium borodeuteride treatment of urine expected to have large concentrations of pyruvic and acetoacetic acids produces lactic and 3-hydroxybutyric acids labelled with deuterium. Mass fragmentographic techniques applied to the trimethylsilyated extract of the urine spiked with more extensively labelled analogues as internal standards permit the reliable determination of lactic, pyruvic, 3-hydroxybutyric and acetoacetic acids in a single analysis.
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McGill University Medical Research Council of Canada Biomedical Mass Spectrometry Unit, Montreal, Quebec, Canada.
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000097:blood / Q000652:urine*; D000328:Adult; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006885:Hydroxybutyrates / Q000097:blood / Q000138:chemical synthesis / Q000652:urine*; D007231:Infant, Newborn; D007553:Isotope Labeling; D007662:Ketosis / Q000652:urine; D007773:Lactates / Q000097:blood / Q000138:chemical synthesis / Q000652:urine*; D011773:Pyruvates / Q000097:blood / Q000652:urine*; D019289:Pyruvic Acid
D000090:Acetoacetates; D006885:Hydroxybutyrates; D007773:Lactates; D011773:Pyruvates; C016635:acetoacetic acid; D019289:Pyruvic Acid; D003903:Deuterium; D020155:3-Hydroxybutyric Acid