title: The major allergen of the house dust mite, Dermatophagoides pteronyssinus, is synthesized and secreted into its alimentary canal.
abstract: The natural production of Der pl, the major faecal allergen from the house dust mite (Dermatophagoides pteronyssinus), was investigated. Mite cultures grown on radiolabelled substrate were examined at intervals over a period of 21 days and incorporation of radiolabel into the mite proteins measured over the time course. Mite proteins were extracted, purified by chromatofocussing and characterized by immunodiffusion, SDS-PAGE, electroblotting and autoradiography. The faecal allergen, Der pl, did not incorporate a significant level of label until the 14th day (p less than 0.05). The results suggest that Der pl is a protein synthesized and excreted by the house dust mite.
authors.affiliation: Department of Child Health, University of Bristol, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Thompson
authors.forename: F
authors.initials: F
authors.lastname: Carswell
mesh_terms: D000485:Allergens / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D039741:Antigens, Dermatophagoides; D004064:Digestive System / Q000276:immunology; D005243:Feces / Q000276:immunology; D008925:Mites / Q000276:immunology* / Q000378:metabolism
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides