Hormonal effects of lead acetate in the male rat: mechanism of action.
Environmental exposure to toxic levels of lead occurs in a number of industries with potential adverse effects on the reproductive capacity of exposed men. Using a rat model, we previously reported that dietary exposure to lead resulted in suppressed spermatogenesis and testosterone levels without significant changes in luteinizing hormone (LH). In this study, to identify more specifically the site of lead's toxic actions on the hypothalamic-pituitary-testicular axis, the response of lead-treated male rats as compared to control animals to naloxone, gonadotropin-releasing hormone (GnRH), and LH stimulation was studied. Three groups of 52-day-old Wistar rats were allowed access to either deionized distilled water containing no lead acetate or a 0.3% lead acetate solution for 30 days. In each study group, 10 control and 10 lead-treated animals were anesthetized prior to cardiac puncture and collection of serum for the measurement of lead level and baseline LH (Groups I and II) or testosterone levels (Group III). In Group I, 20 min after an i.p. injection of naloxone (1.5 mg/kg/BW), the animals were killed by decapitation, and serum was collected for LH measurement. Thirty minutes after an i.p. injection of GnRH (100 ng/100 gm BW), Group II animals were killed by decapitation, and serum was collected for LH. Sixty minutes after an injection of LH (100 mg/100 mg BW), serum was collected from Group III animals for testosterone measurement. All control animals and lead-treated animals consumed similar volumes of water. Control animals had undetectable levels of lead in their blood. Lead-treated animals had mean blood lead values of 30 micrograms/dl +/- 5 micrograms/dl.(ABSTRACT TRUNCATED AT 250 WORDS)
Sokol
R Z
RZ
Department of Medicine, Harbor-UCLA Medical Center, Torrance 90509.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects*; D007854:Lead / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood / Q000494:pharmacology; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D009942:Organometallic Compounds / Q000633:toxicity*; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000187:drug effects*; D013739:Testosterone / Q000097:blood
D009942:Organometallic Compounds; D010906:Pituitary Hormone-Releasing Hormones; D007854:Lead; D009270:Naloxone; D013739:Testosterone; D007986:Luteinizing Hormone; C008261:lead acetate