Expression of c-myc during differentiation of the human teratocarcinoma cell line Tera-2.
The quantity of c-myc mRNA was measured during the retinoic-acid-induced differentiation of the pluripotent human teratoma cell line, Tera-2 cl. 13. As the cells were exposed to retinoic acid for longer periods of time the duration of the cell cycle progressively increased (measured by the rate of S phase entry) until the cells were effectively quiescent and expressed characteristic differentiation markers. Under these circumstances steady-state levels of c-myc expression increased by up to 1.6-fold with respect to rapidly growing undifferentiated cells. Southern blot analysis of the c-myc genes in Tera-2 indicated no major rearrangement or amplification in the cell line.
Schofield
P N
PN
Department of Zoology, University of Oxford, UK.
Engstrom
W
W
Lee
A J
AJ
Biddle
C
C
Graham
C F
CF
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D014212:Tretinoin