Agrobacterium-mediated infectivity of cloned digitaria streak virus DNA.
A monomeric clone of double-stranded DNA synthesized in vitro DNA of the geminivirus Digitaria streak (DSV) was subcloned as a tandem dimeric unit into a binary vector of Agrobacterium tumefaciens, creating a plasmid pDS2. Inoculation of digitaria sanguinalis with A. tumefaciens carrying pDS2 resulted in viral infection. The symptoms, virus particles, and DNA forms obtained were indistinguishable from those of a natural DSV infection of D. sanguinalis. Inoculations have also induced infections in Zea mays and Avena sativa. The sequence of the Agrobacterium-mediated infectious clone of DSV has been determined.
Donson
J
J
John Innes Institute, AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, United Kingdom.
Gunn
H V
HV
Woolston
C J
CJ
Pinner
M S
MS
Boulton
M I
MI
Mullineaux
P M
PM
Davies
J W
JW
D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors; D010942:Plant Viruses / Q000235:genetics* / Q000502:physiology; D010944:Plants / Q000382:microbiology*; D012231:Rhizobium / Q000235:genetics
D004279:DNA, Viral