Generation and characterization of a murine monoclonal antibody specific for the human T1-CD5 molecule.
Murine monoclonal antibodies (MoAbs) have found widespread applications in the characterization of the molecular and functional features of lymphocyte differentiation antigens. The present paper summarizes the results of our work dealing with the production and selection of a murine MoAb recognizing a molecule expressed during the whole differentiative life of T lymphocytes. The MoAb CB01 resulted to be specific for an apparently unique epitope of the T-cell specific membrane glycoprotein T1-CD5.
Genzano
F
F
Dipartimento di Genetica, Biologia e Chimica Medica, Università di Torino, Italy.
Funaro
A
A
Alessio
M
M
De Monte
L B
LB
Bellone
G
G
Peruzzi
L
L
Zaccolo
M
M
Caligari-Cappino
F
F
Malavasi
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte