Immunological and molecular studies in a case of follicular lymphoma with an extra chromosome 12 and t(2;8) translocation.
Two sequential lymph node biopsies taken from a non-Hodgkin lymphoma patient revealed two karyotype abnormalities peculiar to B cell neoplasias: trisomy 12 and t(2;8)(p12;q24) translocation. The first was documented in all cells analyzed, while the second was present in 20% of the metaphases from the first biopsy and in 100% from the second. This suggests that the t(2;8) translocation arose as a secondary karyotypic change. In addition, although immunological characterization of the neoplastic cells disclosed a monoclonal B cell population that expressed immunoglobulin kappa light chains, as usually found in Burkitt's lymphoma with t(2;8) translocation, Southern blot analysis provided evidence of rearrangement in only one kappa chain allele.
Donti
E
E
First Institute of Internal Medicine, University of Perugia, Italy.
Falini
B
B
Giuseppe Pelicci
P
P
Venti Donti
G
G
Rosetti
A
A
Martelli
M
M
Grignani
F
F
D002869:Chromosome Aberrations / Q000150:complications*; D025063:Chromosome Disorders; D002881:Chromosomes, Human, Pair 12; D006801:Humans; D007621:Karyotyping; D008223:Lymphoma / Q000150:complications / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008967:Molecular Biology; D014178:Translocation, Genetic