In vitro malignant transformation of in vivo ENU-induced rat ovarian Sertoli cell tumor (adenoma).
An N-ethyl-N-nitrosourea-induced rat ovarian Sertoli cell tumor was grown in tissue culture in Dulbecco's modified Eagle's medium (DMEM) supplemented with 25% horse serum (HS) and a hormone combination of 20 ng/ml each of hydrocortisone, insulin, and prolactin. This tissue culture derived from a nonsteroid hormone-producing tumor. Cytofluorometry and karyotyping of the nonhormone-producing tumor cell line (SCTL-1) revealed a diploid pattern for the early passage (P1), which became hyperdiploid (P10), and then aneuploid (P20). These cells had an epitheloid pattern, grew in a monolayer at early passages. After P10 the cells were transplanted into newborn rats and nude mice and resulted in high incidences of tumors (up to 100%). The cell line (SCTL-1) continued to grow in DMEM, 10% HS, and no hormone supplementation after P10. This study revealed that a benign rat ovarian Sertoli cell tumor after multiple passages in vitro underwent sequential genotypic and phenotypic changes and became highly malignant.
Stoica
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G
Texas A&M University, Department of Veterinary Pathology, College Station 77843.
O'Leary
M
M
D000236:Adenoma / Q000473:pathology*; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis; D005038:Ethylnitrosourea; D005260:Female; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D051381:Rats; D012707:Sertoli Cell Tumor / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D004247:DNA; D005038:Ethylnitrosourea