Selective decrease in low-Mr HMG proteins HMG I and HMG Y during differentiation of mouse teratocarcinoma cells.
We have studied the presence of high-mobility-group (HMG) chromatin proteins in undifferentiated F9 mouse teratocarcinoma cells and F9 cells, which were induced to differentiate by treatment with retinoic acid and dibutyryl-cAMP for 5 days. Acetic acid/urea-polyacrylamide gel electrophoresis and reversed-phase HPLC revealed that the induced F9 cells contained 77 and 62% less HMG I and HMG Y, respectively, than their untreated counterparts. The relative amounts of two other low-Mr HMG proteins HMG 14 and HMG 17 remained essentially unchanged and only a minor decrease was observed in the content of one of the high-Mr HMG proteins, HMG 2. The identity of the low-Mr HMG proteins was verified by amino acid analysis or partial sequencing. These results suggest that HMG I and HMG Y are HMG proteins specific for undifferentiated cells.
Vartiainen
E
E
Department of Biochemistry, University of Kuopio, Finland.
Palvimo
J
J
Mahonen
A
A
Linnala-Kankkunen
A
A
Mäenpää
P H
PH
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins / Q000032:analysis*; D051379:Mice; D012995:Solubility; D013724:Teratoma / Q000032:analysis*; D013820:Thermolysin / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis
D006609:High Mobility Group Proteins; D013820:Thermolysin