Flow cytometric evaluation of the effects of 2.3 MeV neutrons and 240 kV X rays on mouse spermatogenic S-phase cells using bromodeoxyuridine incorporation.
A sensitive flow cytometric assay has been developed to assess the effect of X rays or neutrons on the DNA-synthesizing ability of mouse testis cells. The dose to reduce the labelling index (LI) to 50% (ED50) was 25 cGy for X rays and 5 cGy for neutrons, giving a relative biological effectiveness (RBE) of 5.0. A significant reduction of the LI was seen at X-ray doses as low as 10 cGy. Neutron treatment resulted in an exponential decrease in functional S-phase cells while the X-ray response was biphasic. The sensitivity and rapidity of the assay would be suitable for use in biological dosimetry.
Gasinska
A
A
Cancer Research Campaign Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex.
Wilson
G D
GD
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D004247:DNA / Q000096:biosynthesis; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry / Q000379:methods*; D007399:Interphase / Q000528:radiation effects; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009502:Neutrons; D011839:Radiation, Ionizing; D013091:Spermatogenesis / Q000528:radiation effects*; D013737:Testis / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D014965:X-Rays
D004247:DNA; D001973:Bromodeoxyuridine