[Cell wall permeability and export of proteins into the culture broth of Saccharomyces cerevisiae].
The upper limit of Saccharomyces cerevisiae cell wall permeability is such that only molecules with a mass not greater than 2-3 kDa can pass across the cell wall. However, proteins with a much higher molecular mass are exported into the cultural broth when the organism is cultivated in a liquid medium with peptone. Under certain conditions, exogenous proteins interact with the cytoplasmic membrane. These as well as some other findings imply that the cell wall may have microplots with an anomalous permeability, via which the regulated exchange of macromolecules is realized between the intracellular and outer media.
Tsiomenko
A B
AB
Lupashin
V V
VV
Dmitriev
V V
VV
Kulaev
I S
IS
D000135:Acid Phosphatase / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005656:Fungal Proteins / Q000378:metabolism*; D006651:Histocytochemistry; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D003470:Culture Media; D005656:Fungal Proteins; D046911:Macromolecular Substances; D003208:Concanavalin A; D000135:Acid Phosphatase
Pronitsaemost' kletochnoĭ stenki i éksport belkov v kul'tural'nuiu zhidkost' u Saccharomyces cerevisiae.