Electrophoretic pattern of NADPH-dependent oxidoreductive activities in K 562 and HL 60 leukemic cell lines.
Morphological and functional differentiation of hemopoietic cells is accompanied by the expression of lineage-specific protein markers. NADPH-oxidoreductive enzymatic activities in HL 60 and K 562 leukemic cell lines, compared with granulocytes and erythrocytes, show a NADPH-oxidizing and a NADPH-diaphorase activity. The oxidizing activity, absent in erythrocytes, has the same electrophoretic migration in HL 60 cells and granulocytes while it is different in K 562 cells. The diaphorase, absent in HL 60 cells and granulocytes, has the same migration in erythrocytes and K 562 cells, although with a slightly different quantitative expression. K 562 cells induced to differentiation with arabinofuranosylcytosine show the appearance of a band of NADPH-oxidizing activity of granulocytic type, together with the major band found in these cells.
Ravazzolo
R
R
Department of Biology, I.S.M.I., University of Genoa, Italy.
Barresi
R
R
Garrè
C
C
Bianchi-Scarrà
G
G
Ajmar
F
F
D002454:Cell Differentiation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology; D006098:Granulocytes / Q000166:cytology / Q000201:enzymology; D006801:Humans; D066298:In Vitro Techniques; D007942:Leukemia, Experimental / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000201:enzymology*
D009247:NADH, NADPH Oxidoreductases