Inhibition by the mushroom toxins alpha-amanitin and phalloidin of hepatopoietin-induced 3H-thymidine incorporation into rat liver DNA and of plasma protein production in hepatocyte cultures.
In primary cultures of rat hepatocytes the induction of 3H-thymidine uptake into DNA of liver cells by the liver cell proliferation factor hepatopoietin demonstrates that this factor is active not only in vivo but also in vitro. Addition of the mushroom toxins alpha-amanitin or phalloidin to liver cell culture decreased the uptake of 3H-thymidine into hepatocytes (in the absence or presence of hepatopoietin) as well as the attachment of the hepatocyte cultures. Mushroom toxins also inhibited the production of plasma proteins in hepatocyte cultures. The inhibition, observed at toxin concentrations from 10(-5) to 10(-7) M, was dose-dependent. At low concentrations of phalloidin the inhibition appears to be selective for certain proteins.
Fouad
F M
FM
Department of Food Science and Agricultural Chemistry, McGill University, Ste Anne de Bellevue, Quebec, Canada.
Marshall
W D
WD
Farrell
P G
PG
Goldberg
M
M
Ruhenstroth-Bauer
G
G
D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D006133:Growth Substances / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors; D007122:Immunoelectrophoresis; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000494:pharmacology*; D010590:Phalloidine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism
D000546:Amanitins; D001798:Blood Proteins; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D009842:Oligopeptides; D010590:Phalloidine; D004247:DNA; D013936:Thymidine