The primary structure of human platelet profilin: reinvestigation of the calf spleen profilin sequence.
The primary structure of human platelet profilin was determined by aligning the sequences of its tryptic peptides to the previously determined calf spleen profilin sequence [(1979) FEBS Lett. 101, 161-165]. Comparison of the peptide fingerprints of the two proteins suggested a higher homology than that found by direct sequence comparison. We therefore reinvestigated the sequences of the peptides from calf spleen profilin. We identified four incorrect charge assignments and a deletion of three residues. The similarity between the two vertebrate profilins amounts to 95%.
Ampe
C
C
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Markey
F
F
Lindberg
U
U
Vandekerckhove
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D002417:Cattle; D003285:Contractile Proteins; D004590:Electrophoresis, Paper; D006801:Humans; D008840:Microfilament Proteins / Q000032:analysis; D051304:Profilins; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000032:analysis*
D003285:Contractile Proteins; D008840:Microfilament Proteins; C495738:PFN1 protein, human; D051304:Profilins