Validation of viscosity measurements for canine hepatic bile.
This study developed the validated standards for a known viscosity method by obtaining consistent viscosity measurements with defined precision, reproducibility and sensitivity limits for canine hepatic bile. Size 75 Cannon-Manning semimicro viscometers yielded the most precise viscosity measurements. With the development of proper handling methods for fresh bile samples, a precision defined by a coefficient of variation of less than or equal to +/- 0.3% was obtained. To maintain viscometer constancy, the viscometers must be cleaned with chromic acid after each bile test and pass reproducibility tests using defined distilled water viscosity tests.
Wilson
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Department of Pharmacology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20307-5100.
Lowensohn
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D000818:Animals; D001646:Bile; D002138:Calibration; D002627:Chemistry, Physical / Q000295:instrumentation; D004285:Dogs; D012016:Reference Values; D014783:Viscosity