Purification of Par j I, the major allergen of Parietaria judaica pollen.
A component of Parietaria judaica pollen extract, previously identified as the major allergen, then reported as Pj10 and hereafter denominated Par j I has been isolated by a combination of 65% ammonium sulphate salt precipitation and gel filtration and an Ultrogel AcA54 column. The purified allergen appeared essentially homogeneous on gel filtration HPLC. The mol. wt of Par j I was estimated by electrophoretic and chromatographic techniques. All results gave values in a range from 13 K to 25 K. Analysis in SDS-PAGE under reducing conditions revealed a broad band corresponding to a mol. wt of 10 K, which retained allergenicity when tested with a patients serum pool. CIE and CRIE patterns of the isolated Par j I displayed the two precipitating lines already reported as those exhibiting the highest IgE-binding ability. Par j I showed a specific allergenic activity about 10-fold higher than that of the whole extract and was demonstrated to be the major allergen of Parietaria judaica as assessed in 25 sensitive human sera.
Ayuso
R
R
Departamento de Investigación Biológica, Alergia e Inmunologia Abelló S.A., Madrid, Spain.
Polo
F
F
Carreira
J
J
D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D000645:Ammonium Sulfate; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007123:Immunoelectrophoresis, Two-Dimensional; D007073:Immunoglobulin E / Q000378:metabolism; D008970:Molecular Weight; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test
D000485:Allergens; D007073:Immunoglobulin E; D000645:Ammonium Sulfate