An adaptation of the Candida albicans phototoxicity test to demonstrate photosensitizer action spectra.
The Candida albicans assay is widely used for the testing of potential photosensitizers, especially furocoumarins. A novel adaptation of this assay is described which provides a rapid, inexpensive and easy-to-perform method of producing action spectra. The use of filter paper rectangles with central apertures instead of the usual discs, and monochromatic irradiation with a liquid light guide reduces some of the problems associated with the standard assay. Action spectra for 8-methoxypsoralen and a new pyrrolocoumarin derivative are presented as illustrative examples.
Gibbs
N K
NK
Department of Photobiology, Institute of Dermatology, UMDS, London, UK.
D002176:Candida albicans / Q000187:drug effects* / Q000528:radiation effects; D003374:Coumarins / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008730:Methoxsalen / Q000494:pharmacology; D010787:Photosensitivity Disorders / Q000139:chemically induced*
D003374:Coumarins; C055397:4-methyl-N-ethyl pyrrolo(3,2-g)coumarin; D008730:Methoxsalen