Enzymatic synthesis of p-nitrophenyl N,N',N'',N'',N''''-pentaacetyl-beta-chitopentaoside in water-methanol system; significance as a substrate for lysozyme assay.
Transchitooligosylation from (GlcNAc)5 to the 4-position of PNP-GlcNAc was efficiently induced through lysozyme catalysis in an aqueous solution containing methanol with a high concentration. Use of the aqueous methanol system in this reaction not only guaranteed solubility of PNP-GlcNAc substrate, but also resulted in a remarkable increase in PNP-(GlcNAc)5 production. PNP-(GlcNAc)5 was substrate for lysozyme assay compared with PNP-(GlcNAc)4.
Usui
T
T
Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Hayashi
Y
Y
Nanjo
F
F
Ishido
Y
Y
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000138:chemical synthesis / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol; D009113:Muramidase / Q000032:analysis / Q000378:metabolism*; D009844:Oligosaccharides; D010047:Ovalbumin / Q000032:analysis; D013379:Substrate Specificity; D014867:Water
D005960:Glucosides; D006027:Glycosides; D009844:Oligosaccharides; D014867:Water; C055288:4-nitrophenyl-N,N',N'',N''',N''''-pentaacetyl-beta-chitopentaoside; C014124:4-nitrophenyl-N-acetyl-2-deoxyglucopyranoside; D010047:Ovalbumin; D009113:Muramidase; D000117:Acetylglucosamine; D000432:Methanol