title: Effects of culture conditions on susceptibility of alveolar epithelial cell monolayers to NO2.
abstract: The effects of nitrogen dioxide (NO2) exposure on primary cultured monolayers of rat type II pneumocytes were investigated as a function of the isolation and culture conditions. Monolayers were cultured in Eagle's minimum essential medium (MEM) and in MEM supplemented with Ham's F-12; in some experiments, the initial cell suspension was also replated after 3 h. Both supplementation of the basal medium and replating increased the sensitivity of the monolayers to NO2, as measured by reduction in dome formation of plastic dishes 24 h post-exposure. These findings suggest that comparisons of in vitro toxicologic observations may be complicated by the effects of specific experimental conditions.
authors.affiliation: School of Public Health, University of California, Los Angeles.
authors.forename: J M
authors.initials: JM
authors.lastname: Cheek
authors.forename: E M
authors.initials: EM
authors.lastname: Postlethwait
authors.forename: E D
authors.initials: ED
authors.lastname: Crandall
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004848:Epithelium / Q000187:drug effects; D008297:Male; D009585:Nitrogen Dioxide / Q000633:toxicity*; D010084:Oxidation-Reduction; D011650:Pulmonary Alveoli / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003470:Culture Media; D009585:Nitrogen Dioxide