Role of unique consecutive glutamine repeats in active murine interleukin-2 molecule.
Murine interleukin-2 (MIL-2) cDNA deleted of 11 repeats of a CAG sequence, and that encoded from 33Met to 149Gln were inserted into an expression vector carrying an Escherichia coli tryptophan promoter and were expressed in E. coli, respectively. Recombinant MIL-2 deleted of 11 glutamine repeats (MIL-2(-Gln] supported the growth of murine CTLL-2 cells but recombinant MIL-2 initiated from 34Asp (34Asp-MIL-2) did not. The growth of human T-cell blasts was not supported by MIL-2(-Gln) or 34Asp-MIL-2. MIL-2(-Gln) had identical biological and immunological activities with intact MIL-2, but 34Asp-MIL-2 did not. These results suggest that the consecutive glutamine repeats do not play a role in the biological and immunological activities of MIL-2, but that the peptide sequence around them does, and the species hierarchy that MIL-2 does not act on human lymphocyte is not due to the presence of glutamine repeats in MIL-2.
Kashima
N
N
Central Research Laboratories, Ajinomoto Co., Inc., Kanagawa.
Matsui
H
H
Yamasaki
S
S
Kondo
N
N
Hamuro
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D004926:Escherichia coli / Q000235:genetics; D005973:Glutamine; D007376:Interleukin-2 / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011994:Recombinant Proteins; D005973:Glutamine