Serosurvey of Coxiella burnetii infection in dairy goat herds in Ontario. A comparison of two methods of enzyme-linked immunosorbent assay.
Two technical variations of enzyme-linked immunosorbent assay for the detection of antibodies to Coxiella burnetii were compared in this serosurvey on 20 Ontario dairy goat herds. Both a trichloracetic acid extract and a coctoantigen of purified coxiellas were used to sensitize the microtitration plates. Technical differences related to coating pH, serum dilutions tested and interpretation of results. Results agreed in 98.6% of sera examined, the differing sera were in the low titer borderline range. Only 20% of the herds had seroreactors.
Lang
G H
GH
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D003381:Coxiella / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006041:Goats; D006863:Hydrogen-Ion Concentration; D009864:Ontario; D011237:Predictive Value of Tests; D011778:Q Fever / Q000453:epidemiology / Q000662:veterinary*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial