The interaction between native serum albumin and hepatitis B virus.
Purified hepatitis B virus particles were obtained from HBeAg positive serum by sucrose gradient ultracentrifugation and sephadex G-200 gel filtration. These virions formed a precipitation line in counterimmune electrophoresis with anti-albumin antibody, but the reaction could be inhibited by anti-HBs. After two months at 4 degrees C, another precipitating line was formed under the same condition which could not be inhibited by anti-HBs and was, thus, due to free albumin. When that sample was incubated at 37 degrees C overnight, the line of free albumin disappeared. The virion bound albumin was monomeric in non-denaturing gel electrophoresis. These results suggest that a reversible binding between virion and albumin may occur in vivo and does not require chemical modification or cross-linking.
Lu
X Y
XY
Clinical Immunology Research Unit, Jing An Central District Hospital, Shanghai, China.
Yao
G B
GB
Tian
Y F
YF
D003080:Cold Temperature; D003378:Counterimmunoelectrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D006510:Hepatitis B Antibodies / Q000276:immunology; D006515:Hepatitis B virus / Q000378:metabolism*; D006801:Humans; D011233:Precipitin Tests; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013997:Time Factors; D014771:Virion / Q000378:metabolism
D006510:Hepatitis B Antibodies; D012709:Serum Albumin