Effects of culture substrates and normal hepatic sinusoidal cells on in vitro hepatocyte synthesis of Apo-SAA.
Primary hepatocyte cultures synthesize apo-SAA upon stimulation with supernatant from lipopolysaccharide (LPS)-treated macrophages. The matrices on which the hepatocytes were grown influence their basal apo-SAA synthetic capability. Fibronectin was superior. Coculturing hepatocytes with hepatic sinusoidal cells did not adversely affect the ability of hepatocytes to synthesize and secrete apo-SAA into the culture medium. In 72 h, clear islands of endothelial cells nestled in layers of hepatocytes. Both apo-SAA and apo-SAA were made in considerable quantities but no evidence could be obtained that the apo-SAA were free of apo-A-1. The coculturing of hepatocytes with liver sinusoidal cells, the site of ultimate AA deposition, is a first step in establishing an in vitro system for AA amyloidogenesis.
Subrahmanyan
L
L
Department of Pathology, Queen's University, Kingston, Ontario, Canada.
Kisilevsky
R
R
D000818:Animals; D001053:Apolipoproteins / Q000096:biosynthesis*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007728:Kupffer Cells / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA
D001053:Apolipoproteins; D003470:Culture Media; D006133:Growth Substances; D008075:Lipoproteins, HDL; C035165:apolipoprotein SAA