title: Inhibition of calcium release from skeletal muscle sarcoplasmic reticulum by calmodulin.
abstract: The effect of calmodulin on calcium release from heavy sarcoplasmic reticulum isolated from rabbit skeletal muscle was investigated with actively and passively calcium loaded sarcoplasmic reticulum vesicles and measured either spectrophotometrically with arsenazo III or by Millipore filtration technique. The transient calcium-, caffeine- and AMP-induced calcium release from actively loaded sarcoplasmic reticulum vesicles was reduced to 29%, 51% and 59% of the respective control value by 1 microM exogenous calmodulin. Stopped-flow measurements demonstrate that calmodulin reduces the apparent rate of caffeine-induced calcium release from actively loaded sarcoplasmic reticulum. The rate of calcium uptake measured in the presence of ruthenium red, which blocks the calcium release channel, was not affected by calmodulin or calmodulin-dependent phosphorylation of sarcoplasmic reticulum vesicles with ATP[S]. The rate of the calcium-, caffeine- and AMP-induced calcium release from passively loaded sarcoplasmic reticulum vesicles was reduced 1.4-2.0-fold by 1 microM exogenous calmodulin, i.e. the half-time of release was maximally increased by a factor of two, whilst calmodulin-dependent phosphorylation of a 57 kDa protein with ATP[S] had no effect. The data indicate that calmodulin itself regulates the calcium release channel of sarcoplasmic reticulum.
authors.affiliation: University of Vienna, Pharmakologisches Institut, Vienna, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Plank
authors.forename: W
authors.initials: W
authors.lastname: Wyskovsky
authors.forename: M
authors.initials: M
authors.lastname: Hohenegger
authors.forename: G
authors.initials: G
authors.lastname: Hellmann
authors.forename: J
authors.initials: J
authors.lastname: Suko
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000494:pharmacology*; D002458:Cell Fractionation; D007700:Kinetics; D009132:Muscles / Q000378:metabolism*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D002147:Calmodulin; D002110:Caffeine; D000249:Adenosine Monophosphate; D002118:Calcium