Perturbation of intracellular pH by DIDS on endocytosis of transferrin and iron uptake in rabbit reticulocytes.
DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid) inhibited transferrin and iron uptake by rabbit reticulocytes in a dose-dependent manner. Measurement of intracellular pH shows that DIDS neutralized the pH in the intracellular vesicles and at the same time, rendered the cytoplasmic pH more acidic. The latter may be the result of a perturbance in the acid-extruding mechanism(s) by DIDS, a known anion exchange blocker. Examination of the single-cycle endocytosis of transferrin with pulse-chased technique suggests that DIDS retarded transferrin internalization, iron unloading and transferrin receptor recycling in the cells.
Chan
R Y
RY
Department of Physiology, Medical Faculty, University of Hong Kong.
Loh
T T
TT
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D014168:Transferrin / Q000378:metabolism*
D013267:Stilbenes; D014168:Transferrin; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D007501:Iron; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid