The two transcription units of the autonomous parvovirus minute virus of mice are transcribed in a temporal order.
Using quantitative RNase protection assays, we have monitored the appearance of mRNAs generated during lytic infection of tightly synchronized murine cells by the autonomous parvovirus minute virus of mice. Our results demonstrate that transcripts from the P4 promoter can be detected prior to those from the P39 promoter, providing direct evidence for a temporal order of expression between the two parvovirus promoters.
Clemens
K E
KE
Department of Microbiology, School of Medicine, University of Missouri-Columbia 65212.
Pintel
D J
DJ
D000818:Animals; D002460:Cell Line; D005814:Genes, Viral; D008915:Minute Virus of Mice / Q000235:genetics*; D010321:Parvoviridae / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012260:Ribonucleases; D014158:Transcription, Genetic
D012333:RNA, Messenger; D012367:RNA, Viral; D012260:Ribonucleases