title: Effects of transplantation and age on immunohemopoietic cell growth in the splenic microenvironment.
abstract: Intact spleens from young adult and aged mice were transplanted into young recipients to compare effects of age and effects of spleen transplantation on hemopoietic and immune functions. Hemopoietic functions of histocompatible spleen transplants were assessed by partial cures of genetically anemic WBB6F1-Sl/Sld recipients, and immune functions were measured as numbers of anti-SRBC PFC(sheep red blood cell plaque-forming cells) and responses to the mitogen PHA (phytohemagglutinin). Spleens from WCB6F1 and WBB6F1 donors at least 28 months old partially corrected anemias in 10 of 28 Sl/Sld recipients, whereas spleens from 5- to 10-month-old donors performed significantly better, partially correcting anemias in 22 of 31 Sl/Sld recipients. B6D2F1 spleens were transplanted from either old or young donors in B6D2F1 recipients to test their ability to support immune-responsive cells. These spleen grafts were much smaller than recipient spleens and contained few anti-SRBC PFC. In contrast WCB6F1-+/+ spleens transplanted in Sl/Sld recipients were much larger, weighing more than the intact spleens of the recipients. Nevertheless when these spleens were from young donors, they contained only about 10% as many anti-SRBC PFC and PHA-responsive cells as did recipient spleens, whereas old donor spleens contained even fewer. Use of splenectomized Sl/Sld recipients did not alter these results. Apparently the effect of transplantation was much more important than age in reducing the spleens' abilities to support immune-responsive cells.
authors.affiliation: Jackson Laboratory, Bar Harbor, ME 04609.
authors.forename: D E
authors.initials: DE
authors.lastname: Harrison
authors.forename: C M
authors.initials: CM
authors.lastname: Astle
authors.forename: J
authors.initials: J
authors.lastname: DeLaittre
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology*; D002455:Cell Division; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology* / Q000637:transplantation; D014182:Transplantation, Autologous / Q000009:adverse effects*