title: Redistribution and internalization of antibodies to galactocerebroside by oligodendroglia.
abstract: Glial cultures from mouse brain were used to examine the direct interaction between oligodendroglia and antibodies to galactocerebroside (GalC). The external surface of oligodendroglial membrane sheets showed large GalC+ patches separated by a GalC- network after exposure to anti-GalC and fluoresceinated second antibody at 37 degrees C. However, the membrane sheets were evenly stained when cultures were fixed prior to staining or stained at 0 degrees C. Further, exposure to second antibody at 0 degrees C or to Fab fragments of second antibody at 37 degrees C also gave even staining. These results indicate that GalC is normally distributed evenly on the surface of oligodendroglial membrane sheets, but that redistribution or patching of GalC:anti-GalC complexes occurs by cross-linking with second antibody at 37 degrees C. Antibodies to GalC are internalized rapidly and specifically by oligodendroglia. This process is temperature-dependent, and, in contrast to patching, does not require the presence of second antibody. Internalized antibodies are seen within 1 min throughout oligodendroglial membrane sheets, then aggregate in the sheets and in large vein-like structures leading to the cell body. By 1 hr, the cell body is densely packed with vesicles containing anti-GalC. By 24 hr after a 10 min exposure to anti-GalC, internal antibody has disappeared. Anti-GalC is recycled to the cell surface within 5 min after exposure, and continues to appear on the surface for at least 4 hr after the pulse.
authors.affiliation: Department of Neurology, Wayne State University School of Medicine, Detroit, Michigan 48201.
authors.forename: C A
authors.initials: CA
authors.lastname: Dyer
authors.forename: J A
authors.initials: JA
authors.lastname: Benjamins
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002554:Cerebrosides / Q000276:immunology*; D005699:Galactosylceramides / Q000276:immunology*; D051379:Mice; D009457:Neuroglia / Q000276:immunology*; D009836:Oligodendroglia / Q000276:immunology*
chemical_list: D000906:Antibodies; D002554:Cerebrosides; D005699:Galactosylceramides