Study of different phases in growth cycle of human bone marrow cells and their growth speed, using rat-human cell hybridization, premature chromosome condensation, and sister chromatid differentiation techniques.
The cytokinetic activity of bone marrow cells is an indication of hemopoietic status. In the routine examination of bone marrow smears, only M phase can be identified, whereas the different phases of the remainder of the cell cycle are not possible to determine. However, by using premature chromosome condensation and bromodeoxyuridine differential staining techniques, cell stage and cell kinetics can be easily determined. Ninety-eight samples of human bone marrow were collected, cells were hybridized with mitotic M3 cells, and 5016 randomly selected hybrid cells were analyzed for cell stage. Our data demonstrated that 80.37% of the cells were in G1 phase, 12.66% were in S phase, 5.29% were in G2 phase, and 1.68% were in M phase. In specimens that had been cultured for 48 h in the presence of bromodeoxyuridine, 57.46% had completed at least two rounds of DNA replication. In 72-h specimens, 69.27% of the cells had completed at least two S phases. In 96-h specimens, 2S and 2S+ cells occupied 74% of the cell population. The use of cell hybridization techniques, premature chromosome condensation, and bromodeoxyuridine-dependent differential staining revealed the phases of the cell cycle distinctly, as well as the kinetics of cell growth.
Zhang
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S
Cell Research Laboratory, PLA General Hospital, Beijing, China.
Zhang
S Y
SY
Wang
Y F
YF
Ma
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X
D000818:Animals; D001854:Bone Marrow Cells; D002453:Cell Cycle; D006801:Humans; D006824:Hybridization, Genetic; D007399:Interphase; D008677:Metaphase; D051381:Rats; D012854:Sister Chromatid Exchange