Fluorescent inhibitors of a cell surface protease used to locate leukaemia cells in kidney sections.
Guanidinobenzoatase is a trypsin-like protease on the surface of cells capable of migration, for example leukaemia cells. We have used a number of fluorescent probes that are competitive inhibitors of guanidinobenzoatase to locate leukaemia cells in resin sections of kidney tissue obtained from leukaemic rats. We have demonstrated how this competitive inhibition system can be used to direct desired molecules (such as cytotoxic drugs) to these cells and to monitor the arrival of such compounds at the active site of guanidinobenzoatase. The principles developed in this study could equally well be applied to other enzymes on other cells provided suitable competitive inhibitors were designed. The presence of an enzyme on the surface of a cell can be used to direct molecules to that cell provided that these molecules contain a functional group that acts as an inhibitor for the chosen enzyme.
Steven
F S
FS
Department of Biochemistry, University of Manchester, UK.
Griffin
M M
MM
Wong
T L
TL
Jackson
H
H
Barnett
F
F
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002462:Cell Membrane / Q000201:enzymology*; D010450:Endopeptidases; D005456:Fluorescent Dyes; D007668:Kidney / Q000473:pathology*; D007942:Leukemia, Experimental / Q000201:enzymology / Q000473:pathology*; D010447:Peptide Hydrolases / Q000032:analysis*; D011480:Protease Inhibitors; D051381:Rats
D015415:Biomarkers; D005456:Fluorescent Dyes; D011480:Protease Inhibitors; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases; D010447:Peptide Hydrolases