Characterization of commercially available plasminogen preparations in vitro: purity and reactivity.
Three different commercially available plasminogen preparations (Immuno-, Kabi-, and Behring-plasminogens) were examined regarding purity and reactivity to different activators (high molecular weight [HMW] or low molecular weight [LMW] two-chain urokinase type plasminogen activator [tcu-PA], single chain urokinase type plasminogen activator [scu-PA], tissue type plasminogen activator [t-PA], and streptokinase [SK]). The Immuno-preparation was a Lys-plasminogen, commercially available for therapeutical use, whereas the research reagents for KabiVitrum and Behringwerke were Glu-plasminogen. Activity data provided by the manufacturers correlated well with our findings. Also a good correlation of reactivity to activators measured with a chromogenic substrate and on fibrin plates could be observed. The Immuno-plasminogen showed a minimum contamination with plasmin which has to be taken into consideration for the interpretation for its apparently higher activation by plasminogen activators compared to the plasmin-free plasminogens. Further in vitro and in vivo research has to be performed to find out criteria for a practicable scheme of administration of fibrinolytic agents for therapeutical thrombolysis.
Miyashita
C
C
Department of Clinical Hemostaseology and Transfusion Medicine, University of the Saarland, Homburg, FRG.
Heiden
M
M
Leipnitz
G
G
Pindur
G
G
Wenzel
E
E
D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005342:Fibrinolysis; D006801:Humans; D007202:Indicators and Reagents / Q000302:isolation & purification / Q000592:standards; D010446:Peptide Fragments / Q000302:isolation & purification; D010958:Plasminogen / Q000302:isolation & purification* / Q000592:standards / Q000627:therapeutic use; D010960:Plasminogen Activators / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate
D007202:Indicators and Reagents; D010446:Peptide Fragments; C018998:lysyl-plasminogen; D012967:Sodium Dodecyl Sulfate; D010958:Plasminogen; D010960:Plasminogen Activators