Expression of transfected human CuZn superoxide dismutase gene in mouse L cells and NS20Y neuroblastoma cells induces enhancement of glutathione peroxidase activity.
The human CuZn superoxide dismutase (superoxide dismutase 1) a key enzyme in the metabolism of oxygen free-radicals, is encoded by a gene located on chromosome 21 in the region 21 q 22.1 known to be involved in Down's syndrome. A gene dosage effect for this enzyme has been reported in trisomy 21. To assess the biological consequences of superoxide dismutase 1 overproduction within cells, the human superoxide dismutase 1 gene and a human superoxide dismutase 1 cDNA were introduced into mouse L cells and NS20Y neuroblastoma cells. Both cell types expressed elevated levels (up to 3-fold) of enzymatically active human superoxide dismutase 1. These human superoxide dismutase 1 overproducers, especially neuronal cell lines, showed an increased activity in the selenodependent glutathione peroxidase. These data are consistent with the possibility that gene dosage of superoxide dismutase 1 contributes to oxygen metabolism modifications previously described in Down's syndrome.
Ceballos
I
I
Laboratoire de Biochimie Génétique, Hôpital Necker, Paris, France.
Delabar
J M
JM
Nicole
A
A
Lynch
R E
RE
Hallewell
R A
RA
Kamoun
P
P
Sinet
P M
PM
D000818:Animals; D002460:Cell Line; D005796:Genes; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D007739:L Cells / Q000201:enzymology; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase