Automated protein crystallization and a new crystal form of a subtilisin:eglin complex.
A procedure is described for automating labour-intensive steps of the 'hanging drop' protein crystallization method. An automatic sample changer is employed to fill the wells in a multi-well plate so that concentration gradients in various components are obtained. The sample changer is also used for preparing droplets on a second multi-well plate. Subsequently, this second plate is manually turned around and placed on top of the first multi-well plate such that a large number of chambers with different conditions is obtained simultaneously. During initial trials a new crystal form of a subtilisin:eglin complex was obtained. The crystals have space group P2(1), contain two enzyme inhibitor complexes per asymmetric unit and diffract beyond 2.2 A.
Kelders
H A
HA
Laboratory of Chemical Physics, University of Groningen, The Netherlands.
Kalk
K H
KH
Gros
P
P
Hol
W G
WG
D001331:Automation; D003460:Crystallization; D011506:Proteins; D015843:Serpins; D013381:Subtilisins
D011506:Proteins; D015843:Serpins; C028656:eglin proteinase inhibitors; D013381:Subtilisins