Osteoclast-like cells grow in cultures of multipotent hematopoietic progenitors in thrombocytopenia and absent radii (TAR) syndrome.
Cultures of bone marrow multipotent hematopoietic progenitors were performed in a case of TAR syndrome and normal bone marrow. It was found that clones of large multinucleated cells formed after 2 weeks of cultures from the TAR bone marrow but not in that of control subjects of the same age. Those cells contained a tartrate-resistant acid phosphatase activity and were negative for both monocytes and megakaryocytic markers. The data suggest that the multinucleated cells have several characteristics of osteoclasts. It was also found that growth of multipotent and megakaryocytic colonies was reduced when compared with normal control subjects (36 and 24%, respectively). This new finding of osteoclast or osteoclast-like cells derived from cultures of bone marrow hematopoietic precursors in TAR syndrome is discussed in relation to the lack of megakaryocytic characteristics in this disorder.
Michalevicz
R
R
Institute of Hematology, Ichilov Hospital, Tel Aviv., Israel.
Baron
S
S
Burstein
Y
Y
D000015:Abnormalities, Multiple / Q000503:physiopathology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006801:Humans; D007223:Infant; D008264:Macrophages / Q000502:physiology; D008533:Megakaryocytes / Q000502:physiology; D010010:Osteoclasts / Q000502:physiology*; D011884:Radius / Q000002:abnormalities; D013234:Stem Cells / Q000502:physiology*; D013577:Syndrome; D013921:Thrombocytopenia / Q000503:physiopathology*