Characterization of Staphylococcus epidermidis mutants sensitive to ultraviolet radiation.
Five UV-sensitive mutants obtained by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) treatment of the Staphylococcus epidermidis W5 strain were characterized phenotypically by assaying their UV- and MNNG-sensitivities, lysogenic inducibility, host-cell reactivation and Weigle reactivation capacities. The results were compared with those of well-characterized Escherichia coli strains, permitting the identification of: 2 mutants that behave as Uvr- Umu-; 1 mutant that appears analogous to Uvr-; 1 mutant that resembles LexA- and 1 mutant that exhibits a RecA- phenotype. The study of these mutants can contribute to the understanding of the repair mechanisms in S. epidermidis.
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Instituto de Biofisica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brasil.
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D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D007700:Kinetics; D008242:Lysogeny / Q000528:radiation effects; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009154:Mutation / Q000528:radiation effects*; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000528:radiation effects*; D014466:Ultraviolet Rays
D008769:Methylnitronitrosoguanidine