[Use of fermenter dialysis culture technic to grow clinically important bacteria which carry capsules. I. Escherichia coli K1].
For mass culture of Escherichia coli K1 a fermenter dialysis culture technique based on an artificial hollowfiber kidney and substrate feeding is described and compared with conventional shaking culture and fermenter batch culture techniques. Important growth parameters as bacterial dry weight, optical density and live cell counts demonstrate the advantage of this new method. On the basis of bacterial yields, effectivity had increased about 100 fold. The synthesis of K1 capsular polysaccharides, which is influenced by a number of factors, proved to be good. Thus it was possible to obtain the quantities of native capsular substance necessary for bed-side diagnostics and the investigation of this virulence marker.
Starke
R
R
Institut für Medizinische Mikrobiologie des Bereiches Medizin (Charité), Humboldt-Universität zu Berlin.
Mehl
M
M
Buddenberg
S
S
Presber
W
W
Jacobi
H D
HD
D000942:Antigens, Bacterial; D016667:Bacterial Capsules; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000254:growth & development* / Q000472:pathogenicity; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D014774:Virulence
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1
Einsatz der Fermenter-Dialyse-Kultur zur Züchtung kapseltragender Bakterien mit klinischer Bedeutung. I. Escherichia coli K1.