title: Antigenic variation of wild and vaccine rabies strains of Egypt.
abstract: Nineteen street rabies virus strains, isolated in Egypt from humans (two), dogs (nine), cats (two), farm animals (two), gerbils (three), and a jackal were antigenically analyzed. The Pasteur strain used for the preparation of human rabies vaccine, the Flury high and low egg passage stains (HEP, LEP) used for animal vaccines, and the challenge virus standard (CVS) strain were also assayed. All were examined by the indirect fluorescent antibody test, using a panel of 20 monoclonal antibodies against the nucleocapsid of rabies and rabies-related viruses. The rabies isolates demonstrated patterns of reactivity with the antinucleocapsid panel different from those of the Pasteur, HEP, and CVS strains. Representative human, dog, and rodent isolates were analyzed by neutralization tests in mice, with a second panel of 19 monoclonal antibodies against rabies and Mokola envelope glycoproteins. With this panel, the isolates demonstrated patterns of reactivity different from the vaccine strains. These data indicate antigenic variation between wild virus and vaccine strains.
authors.affiliation: United States Naval Medical Research Unit No. 3, Cairo, Egypt.
authors.forename: B A
authors.initials: BA
authors.lastname: Botros
authors.forename: A W
authors.initials: AW
authors.lastname: Salib
authors.forename: P W
authors.initials: PW
authors.lastname: Mellick
authors.forename: J M
authors.initials: JM
authors.lastname: Linn
authors.forename: A K
authors.initials: AK
authors.lastname: Soliman
authors.forename: R M
authors.initials: RM
authors.lastname: Scott
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology*; D002415:Cats; D004285:Dogs; D006801:Humans; D009500:Neutralization Tests; D011819:Rabies Vaccines / Q000276:immunology; D011820:Rabies virus / Q000276:immunology* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D011819:Rabies Vaccines; D014759:Viral Envelope Proteins