title: Cloning of human androgen receptor complementary DNA and localization to the X chromosome.
abstract: The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using a consensus nucleotide sequence from the DNA-binding domain of the family of nuclear receptors. The AR gene was localized on the human X chromosome between the centromere and q13. Cloned complementary DNA, selected with an AR-specific oligonucleotide probe, was expressed in monkey kidney (COS) cells and yielded a high-affinity androgen-binding protein with steroid-binding specificity corresponding to that of native AR. A predominant messenger RNA species of 9.6 kilobases was identified in human, rat, and mouse tissues known to contain AR and was undetectable in tissues lacking AR androgen-binding activity, including kidney and liver from androgen-insensitive mice. The deduced amino acid sequence of AR within the DNA-binding domain has highest sequence identity with the progesterone receptor.
authors.affiliation: Department of Pediatrics, University of North Carolina, Chapel Hill 27599.
authors.forename: D B
authors.initials: DB
authors.lastname: Lubahn
authors.forename: D R
authors.initials: DR
authors.lastname: Joseph
authors.forename: P M
authors.initials: PM
authors.lastname: Sullivan
authors.forename: H F
authors.initials: HF
authors.lastname: Willard
authors.forename: F S
authors.initials: FS
authors.lastname: French
authors.forename: E M
authors.initials: EM
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003062:Codon; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D011944:Receptors, Androgen / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
chemical_list: D003062:Codon; D011944:Receptors, Androgen