Effect of cell substrate on antioxidant enzyme activities in cultured renal glomerular epithelium.
The activities of three antioxidant enzymes, superoxide dismutase, catalase, and glutathione peroxidase, were monitored in isolated guinea pig glomeruli and primary or subcultured glomerular epithelial cells. Cell injury was assessed by morphologic studies and by measurement of cellular lipid peroxidation (levels of malondialdehyde). Antioxidant enzyme activities were very different in cultured cells than in parent glomeruli. The possible effect of culture substrates (tissue culture plastic, bovine corneal endothelial [BCE] cell basement membrane, and PF-HR-9 endodermal cell basement membrane) on antioxidant enzyme status, cell morphology, and lipid peroxidation was also assessed. Glomerular epithelial cells cultured on the BCE cell basement membrane substrate survived longer and showed less lipid peroxidation than cells cultured on plastic or the HR-9 substrate. Cells cultured on a plastic substrate had substantially less glutathione peroxidase activity than cells cultured on either BCE or HR-9 basement membranes.
Yang
A H
AH
Department of Pathology, University of Wisconsin School of Medicine, Madison.
Oberley
T D
TD
Oberley
L W
LW
Ramanathan
R
R
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D004848:Epithelium / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000378:metabolism; D006168:Guinea Pigs; D007678:Kidney Glomerulus / Q000166:cytology / Q000201:enzymology*; D008054:Lipid Peroxides / Q000378:metabolism; D008854:Microscopy, Electron; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D003470:Culture Media; D008054:Lipid Peroxides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase