Morphogenetic effects of glucose on mouse islet-cell re-aggregation in culture.
The re-aggregation of dispersed islet cells from non-inbred ob/ob-mice was studied by light and electron microscopy. After 3 days of culture, spontaneously formed aggregates with more than 95% beta-cells were up to 0.5 mm in diameter and exhibited a high degree of viability on dye exclusion tests. In comparison with cultures at 1 or 3 mM D-glucose, or 1 mM D-glucose in combination with 19 mM 3-0-methyl-D-glucose, aggregates formed in 20 mM D-glucose were more closely packed, had a smoother circumference with elongated peripheral beta-cells, and exhibited well developed micro-villi in localized intercellular widenings. A stereological analysis of electron micrographs showed that beta-cells aggregated at 20 mM D-glucose exhibited the same individual profile area but a significantly lower form factor, and a significant reduction in granule volume density as compared with aggregates at 3 mM D-glucose. It is concluded that D-glucose has morphogenetic effects on both the cellular and the micro-anatomical level of pseudo-islet structure in culture.
Norlund
R
R
Department of Histology and Cell Biology, University of Umeå, Sweden.
Norlund
L
L
Täljedal
I B
IB
D000818:Animals; D002449:Cell Aggregation; D002478:Cells, Cultured; D005947:Glucose / Q000008:administration & dosage / Q000502:physiology*; D007515:Islets of Langerhans / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008820:Mice, Obese; D009024:Morphogenesis
D005947:Glucose