title: Structure of tumour necrosis factor by X-ray solution scattering and preliminary studies by single crystal X-ray diffraction.
abstract: The structure of tumour necrosis factor has been investigated by X-ray small-angle scattering and X-ray diffraction using synchrotron radiation. The overall radius of gyration is 25.5 A. A plausible model accounting for the scattering curves consists of an elongated trimer with an axial ratio of 3 to 4 and a maximal chord with a lower limit of 80 A. Tumour necrosis factor has been crystallized in a trigonal space group. Our results are in favour of a single trimer in the asymmetric unit. The diffraction extends to 3.5 A.
authors.affiliation: LURE, Université Paris-Sud, Orsay, France.
authors.forename: A
authors.initials: A
authors.lastname: Lewit-Bentley
authors.forename: R
authors.initials: R
authors.lastname: Fourme
authors.forename: R
authors.initials: R
authors.lastname: Kahn
authors.forename: T
authors.initials: T
authors.lastname: Prangé
authors.forename: P
authors.initials: P
authors.lastname: Vachette
authors.forename: J
authors.initials: J
authors.lastname: Tavernier
authors.forename: G
authors.initials: G
authors.lastname: Hauquier
authors.forename: W
authors.initials: W
authors.lastname: Niers
mesh_terms: D006801:Humans; D008954:Models, Biological; D014409:Tumor Necrosis Factor-alpha; D014961:X-Ray Diffraction
chemical_list: D014409:Tumor Necrosis Factor-alpha