title: Modulation of red cell band 4.1 function by cAMP-dependent kinase and protein kinase C phosphorylation.
abstract: Human erythrocyte protein 4.1 is phosphorylated in vivo by several protein kinases including protein kinase C and cAMP-dependent kinase. We have used cAMP-dependent kinase purified from red cells and protein kinase C purified from brain to test the effects of phosphorylation on band 4.1 function. In solution, each kinase catalyzed the incorporation of 1-4 mol of PO4/mol of band 4.1. Phosphorylation of band 4.1 by each kinase resulted in a significant (50-80%) reduction in the ability of band 4.1 to promote spectrin binding to F-actin. Direct measurement of spectrin-band 4.1 binding showed that phosphorylation by each kinase also caused dramatic reduction in this association. Phosphorylation of band 4.1 by each kinase for increasing time periods enabled us to demonstrate an approximately linear inverse relationship between PO4 incorporation into band 4.1 and spectrin binding. These results show that phosphorylation of band 4.1 by cAMP-dependent kinase and protein kinase C may be central to the regulation of red cell cytoskeletal organization and membrane mechanical properties.
authors.affiliation: Department of Biomedical Research, St. Elizabeth's Hospital, Boston, Massachusetts 02135.
authors.forename: E
authors.initials: E
authors.lastname: Ling
authors.forename: Y N
authors.initials: YN
authors.lastname: Danilov
authors.forename: C M
authors.initials: CM
authors.lastname: Cohen
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D006801:Humans; D008565:Membrane Proteins; D008970:Molecular Weight; D009479:Neuropeptides; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013049:Spectrin / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1; D013049:Spectrin; D011494:Protein Kinases; D011493:Protein Kinase C