Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.
When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.
Shieh
C C
CC
Department of Medicine, Veterans General Hospital, Taipei, Taiwan R.O.C.
Chang
S C
SC
Tzeng
C R
CR
Huang
J J
JJ
Nir
W J
WJ
Hong
C Y
CY
D000328:Adult; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D012463:Saliva / Q000032:analysis*; D012661:Semen / Q000032:analysis*; D013739:Testosterone / Q000032:analysis* / Q000097:blood
D013739:Testosterone