Metabolism of benz[j]aceanthrylene (cholanthrylene) and benz[l]aceanthrylene by induced rat liver S9.
The metabolites of benz[j]aceanthrylene (B[j]A) produced by incubation with liver S9 proteins from rats induced with Aroclor-1254 and phenobarbital have been identified as: trans-B[j]A-1,2-dihydrodiol, B[j]A-9,10-dihydrodiol, B[j]A-11,12-dihydrodiol, and 10-hydroxy-B[j]A. The major metabolite formed (58-60%) by both induced S9 preparations was trans-B[j]A-1,2-dihydrodiol, the cyclopenta-ring dihydrodiol while oxidation at the k-region or the proximal-bay region was minor. There were no statistical differences in individual or total B[j]A metabolite rates between the 2 induced S9 preparations. B[l]A was metabolized by Aroclor-1254 and phenobarbital induced rat liver S9 preparations to trans-B[l]A-1,2-dihydrodiol, B[l]A-7,8-dihydrodiol, and B[l]A-4,5-dihydrodiol. The major B[l]A metabolite formed (28-40%) by both induced S9 preparations was B[l]A-7,8-dihydrodiol, the k-region dihydrodiol. Cyclopenta-ring oxidation to trans-B[l]A-1,2-dihydrodiol was approximately 50% of that observed for k-region oxidation. Both induced S9s produced similar rates of B[l]A metabolites except for B[l]A-7,8-dihydrodiol formation which was higher for Aroclor-1254-induced S9.
Nesnow
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Carcinogenesis and Metabolism Branch, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
Easterling
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Ellis
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Watts
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Ross
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D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001551:Benz(a)Anthracenes / Q000378:metabolism*; D020111:Chlorodiphenyl (54% Chlorine); D004789:Enzyme Activation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008748:Methylcholanthrene / Q000031:analogs & derivatives* / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats
D001140:Aroclors; D001551:Benz(a)Anthracenes; D020111:Chlorodiphenyl (54% Chlorine); D008748:Methylcholanthrene; C041193:benz(l)aceanthrylene; C037411:benz(j)aceanthrylene; D010634:Phenobarbital