Human growth hormone in urine: development of an ultrasensitive radiometric assay.
An immunoradiometric assay for human growth hormone (HGH) has been developed which has a detection limit of 1 ng/l and can measure HGH in unextracted urine from normal children and adults. The assay is based on a two-step procedure, using a solid-phase goat-anti-HGH immunosorbent for immunoextraction and [125I]-labeled monoclonal HGH-antibody for detection and quantification. The assay is not affected by urea, NaCl or changes of pH from 5-8. The mean urine HGH concentration in normal children is 6.78 +/- 7.6 (SD) pg/ml, in patients with HGH-deficiency 1.3 +/- 0.9 pg/ml which increases to 11.7 +/- 13.4 pg/ml on the day of growth hormone injection.
Erb
T
T
University Children's Hospital, Basel, Switzerland.
Karolyi
G
G
Pampalone
A
A
Eberle
A N
AN
Baumann
J B
JB
Bürgisser
E
E
Girard
J
J
D005069:Evaluation Studies as Topic; D013006:Growth Hormone / Q000652:urine*; D006801:Humans; D009994:Osmolar Concentration; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards
D013006:Growth Hormone