Characterisation of protein co-extracted together with LPS in Escherichia coli, Salmonella minnesota and Yersinia enterocolitica.
The porin proteins of Escherichia coli, Yersinia enterocolitica, and Salmonella minnesota were found to co-extract by the phenol-chloroform-petroleum ether method together with the R lipopolysaccharide of these strains. Lipopolysaccharide free protein recovered from the phenolic residue of the phenol-chloroform-petroleum ether extract migrated as a Mr 36-37,000 protein. We could demonstrate that the protein was extracted from bacteria as a high molecular weight protein-lipopolysaccharide complex. Once exposed to phenolic conditions, the protein was no longer soluble in the phenol-chloroform-petroleum ether extraction mixture, indicating a highly specific lipopolysaccharide-protein association.
Strittmatter
W
W
Max-Planck-Institut für Immunbiologie, Freiburg, Federal Republic of Germany.
Galanos
C
C
D000596:Amino Acids / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D004926:Escherichia coli / Q000032:analysis* / Q000276:immunology; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008970:Molecular Weight; D012475:Salmonella / Q000032:analysis* / Q000276:immunology; D013045:Species Specificity; D015008:Yersinia enterocolitica / Q000032:analysis* / Q000276:immunology
D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides