RNA-dependent RNA polymerase activity in two morphologically different white clover cryptic viruses.
RNA-dependent RNA polymerase activities were detected in purified particles of white clover cryptic viruses 1 and 2. The polymerases of the two viruses had different requirements for optimum activity. Enzyme activity was dependent upon the presence of virus particles, Mg2+, and the four ribonucleoside triphosphates, and was insensitive to actinomycin D, alpha-amanitin, and rifampicin. The labeled reaction products were dsRNAs as indicated by CF 11 column chromatography and by their ionic-strength-dependent sensitivity to hydrolysis by RNase A and resistance to S1 nuclease. The dsRNAs synthesized in vitro had the same electrophoretic mobilities as the corresponding viral templates.
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Istituto di Fitovirologia Applicata del CNR, Torino, Italy.
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D010942:Plant Viruses / Q000201:enzymology*; D012316:RNA Nucleotidyltransferases / Q000032:analysis*; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase