title: Clones from two different genomic regions complement the cdc25 start mutation of Saccharomyces cerevisiae.
abstract: We have cloned the CDC25 gene of Saccharomyces cerevisiae whose product is required for traversing the Go phase of the cell cycle. A preliminary physical characterization of the CDC25 gene region is presented. In addition, we show that another gene, when cloned in a high-copy number plasmid, is able to partially suppress growth thermosensitivity of a strain carrying the cdc25 mutation. We briefly discuss the possible interaction of these gene products with adenylate cyclase encoded by the CDC35 gene.
authors.affiliation: Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: J
authors.initials: J
authors.lastname: Daniel
authors.forename: G
authors.initials: G
authors.lastname: Simchen
mesh_terms: D000483:Alleles; D002453:Cell Cycle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005800:Genes, Fungal; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D013489:Suppression, Genetic