Modulation of prolactin responses to gonadotropin releasing hormone by acute testosterone infusions in normal women.
The administration of gonadotropin releasing hormone (GnRH) has been shown to stimulate prolactin (PRL), suggesting its role as an inducer of PRL release. This study addresses whether testosterone may modulate the release of PRL with GnRH during the early follicular phase when this stimulatory effect is not usually observed. Chromatographically pure testosterone was administered intravenously to 13 women in 2 doses (100 micrograms and 1000 micrograms) over a 6-hour period. GnRH (100 micrograms) was administered as a bolus 2 hours before and 4 hours after beginning testosterone. In addition, 3 women received testosterone twice, 3 months apart, with testolactone pretreatment on the second occasion. Serum testosterone rose in all patients and achieved maximum steady-state levels by 120 minutes. Serum estradiol (E2) was increased in subjects receiving the larger dose of testosterone but was unchanged with the lower dose and with the addition of testolactone. PRL did not increase significantly after GnRH before testosterone infusion but showed a significant increase after testosterone as well as after testosterone with testolactone. This effect did not appear to be dose-related.
Serafini
P
P
Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles.
Paulson
R J
RJ
Francis
M M
MM
Lobo
R A
RA
D000293:Adolescent; D000328:Adult; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000097:blood; D005260:Female; D005498:Follicular Phase / Q000187:drug effects; D006801:Humans; D007262:Infusions, Intravenous; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology*; D011388:Prolactin / Q000097:blood*; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*
D010906:Pituitary Hormone-Releasing Hormones; D013739:Testosterone; D004958:Estradiol; D011388:Prolactin