Progesterone and antiprogesterone (RU 38486) modulation of estrogen-inducible glycoprotein (USP-1) synthesis and secretion in rat uterine epithelial cells.
Previous study in this laboratory revealed that estrogen stimulates synthesis and secretion of 97K glycoprotein [uterine secretory protein-1 (USP-1)] in rat uterine epithelial cells. In the present communication, the effects of progesterone and antiprogesterone (RU 38486) on estrogen-stimulated USP-1 biosynthesis were investigated. Ovariectomized rats were treated with estrogen for 4 days to induce USP-1 synthesis and secretion. Then, progesterone and RU 38486 were injected sc. After 6 and 12 h of treatment, uterine luminal fluid proteins were labeled for 6 h by direct administration of [35S]methionine into uterine lumen. By radioimmune precipitation assay using specific antibody against USP-1, it was shown that progesterone can reduce the USP-1 synthesis and secretion, which were prestimulated by estrogen. This antagonistic effect of progesterone on estrogen-induced USP-1 biosynthesis was also evident by the decreased staining intensity of USP-1 in uterine epithelial cells, as revealed by the immunohistochemical method. Although RU 38486 itself did not manifest any effect on estrogen-stimulated USP-1 biosynthesis, it completely inhibited the progesterone-induced decrease in synthesis and secretion. This system is believed to provide a useful model to analyze the progesterone and antiprogesterone actions on rat uterine epithelial cells.
Takeda
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Aichi Cancer Center Research Institute, Nagoya, Japan.
D000818:Animals; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D004963:Estrenes / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins; D007150:Immunohistochemistry; D008715:Methionine / Q000378:metabolism; D015735:Mifepristone; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D051381:Rats; D015843:Serpins; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D004963:Estrenes; D006023:Glycoproteins; D011506:Proteins; D015843:Serpins; C029708:uterine luminal fluid proteins; D015735:Mifepristone; D011374:Progesterone; D008715:Methionine