Purification and some properties of low-molecular-weight proteins of Aspidelaps scutatus (shield or shield-nose snake) venom.
1. Fourteen low-molecular-weight proteins from Aspidelaps scutatus venom were purified by gel filtration and ion exchange chromatography. 2. The proteins (toxins) have i.v. LD50 values ranging from 0.12 to 54 micrograms/g mouse. 3. The amino-terminal sequences of the proteins show that 12 of the 14 proteins were pure. 4. The proteins were preliminarily assigned as phospholipases A2, long neurotoxins and cytotoxin homologues.
Joubert
F J
FJ
National Chemical Research Laboratory, Council for Scientific and Industrial Research, Pretoria, Republic of South Africa.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004546:Elapid Venoms / Q000633:toxicity*; D051379:Mice; D008970:Molecular Weight; D010741:Phospholipases A / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D000596:Amino Acids; C055052:Aspidelaps scutatus venom; D004546:Elapid Venoms; D011506:Proteins; D010741:Phospholipases A