title: Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae. IV. Influence of DNA replication and excision repair on REV2 dependent UV-mutagenesis and repair.
abstract: A double mutant being thermoconditionally defective in mutation induction as well as in repair of pre-lethal UV-induced DNA damage (rev2ts) and deficient in excision repair (rad3-2) was studied in temperature-shift experiments. The influence of inhibitors of DNA replication (hydroxyurea, aphidicolin) was determined. Additionally, an analysis of the dose-response pattern of mutation induction ("mutation kinetics") at several ochre alleles was carried out. It was concluded that the UV-inducible REV2 dependent mutagenic repair process is not induced in excision-deficient cells. In excision-deficient cells, REV2 dependent mutation fixation is slow and mostly post-replicative though not dependent on DNA replication. The REV2 mediated mutagenic process could be separated from the repair function.
authors.affiliation: Gesellschaft für Strahlen- und Umweltforschung, Institut für Strahlenbiologie, Neuherberg, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Siede
authors.forename: F
authors.initials: F
authors.lastname: Eckardt
mesh_terms: D004260:DNA Repair / Q000528:radiation effects; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005800:Genes, Fungal; D006918:Hydroxyurea / Q000494:pharmacology; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D006918:Hydroxyurea