Dimethyl sulfoxide interferes with in vitro differentiation of chick embryo endochondral chondrocytes.
Dedifferentiated chondrocytes derived from 6-day-old chick embryo tibiae when transferred on agarose, revert to the chondrocytic phenotype and mature to hypertrophic, type X collagen-producing chondrocytes (Castagnola et al. (1986). J. Cell Biol. 102, 2310-2317). The continuous presence of 180 mM dimethyl sulfoxide (DMSO) during the culture specifically inhibited synthesis of type X collagen and accumulation of its mRNA. The synthesis of the cartilage-specific type II collagen and the level of its mRNA were essentially unchanged in treated and control untreated cells.
Manduca
P
P
I.S.T., Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Castagnola
P
P
Cancedda
R
R
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D003094:Collagen; D004121:Dimethyl Sulfoxide