Stability of T-2, HT-2, and T-2 tetraol in biological fluids.
The stabilities of tritium-labeled T-2, HT-2, and T-2 tetraol were studied in blood and urine at -70 degrees, 4 degrees, and 23 degrees C for 6 months in the presence of EDTA or NaF. Samples were counted with a radiochromatographic scanner and results indicated the stability of T-2 tetraol greater than T-2 greater than HT-2. Toxins were most stable when stored at -70 degrees C, in the presence of NaF, and in urine (pH 6). They were less stable in saline (control, pH 7) and least stable in blood (pH 8). These results suggest that urine and T-2 tetraol are the biological fluid and metabolite of choice for diagnostic purposes.
Pace
J G
JG
U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21701-5011.
Matson
C F
CF
D000328:Adult; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D012717:Sesquiterpenes / Q000032:analysis*; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine
D012717:Sesquiterpenes; C046700:T-2 tetraol; D013605:T-2 Toxin; C012351:HT-2 toxin