Interaction of bilirubin with the synaptosomal plasma membrane.
The interaction of the neurotoxic pigment bilirubin with synaptosomal plasma membrane vesicles (SPMV) isolated from rat brain was investigated. The interaction seems to involve three steps: (a) a rapid formation of an electrostatic complex between bilirubin and polar lipid head groups; (b) a slow inclusion of the pigment into the hydrophobic core of the membrane; and (c) a SPMV-induced bilirubin aggregation, observed when membrane capacity for bilirubin is exceeded. The association constant of the initial complex increased markedly when pH was lowered below 7.4, particularly in SPMV isolated from newborn rats. A preferential binding of bilirubin to pure gangliosides and sphingomyelin was observed, thus suggesting a role for these lipids as first targets of the pigment in the synaptic membrane. The inclusion of bilirubin into the membranes was gradually enhanced when decreasing the pH or the age of the rats from which SPMV were isolated. In addition, membranes from 2-day-old rats have a higher capacity for bilirubin incorporation compared to those from adult rats. Experiments with reconstituted liposomes of varying protein and cholesterol contents suggest that the effect of age may be related to changes in synaptosomal membrane fluidity during development. Our results support the hypothesis that the interaction of bilirubin with the synaptic membrane plays an important role in the molecular mechanisms of bilirubin neurotoxicity.
Vázquez
J
J
Departamento de Bioquímica y Biología Molecular, Universidad Autónoma de Madrid, Spain.
García-Calvo
M
M
Valdivieso
F
F
Mayor
F
F
Mayor
F
F
D000465:Algorithms; D000818:Animals; D000831:Animals, Newborn; D001663:Bilirubin / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008027:Light; D008958:Models, Molecular; D051381:Rats; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013570:Synaptic Membranes / Q000378:metabolism*; D013997:Time Factors
D001663:Bilirubin