Effect of exercise training on insulin binding and glucose metabolism in mouse soleus muscle.
Training stimulates glucose uptake and metabolism by muscles independent of a rise in serum glucose. Whether this increased insulin action is associated with enhanced insulin binding in muscles is unknown. We studied the effect of 6 weeks of treadmill running on insulin binding, uptake of 2-deoxy-D-glucose, glycolysis, and glycogenesis by the soleus muscle of Swiss Webster mice. Training was progressively increased. The in vitro studies using intact soleus preparations were done 48 h after the last exercise bout. Training increased insulin binding, insulin-stimulated uptake of 2-deoxy-D-glucose, and glycogenesis but not glycolysis in the soleus. Our data suggest that the enhanced glucose uptake and metabolism in muscles induced by exercise training are associated with an increase in insulin binding.
Tan
M H
MH
Department of Medicine, Dalhousie University, Halifax, N.S., Canada.
Bonen
A
A
D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000096:biosynthesis; D006019:Glycolysis; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D008297:Male; D051379:Mice; D009132:Muscles / Q000378:metabolism*; D005082:Physical Exertion
D007328:Insulin; D006003:Glycogen; D003847:Deoxyglucose; D005947:Glucose