N6-cyclohexyladenosine inhibits veratridine-stimulated 22Na uptake by rat brain synaptosomes.
The effect of the stable adenosine analogue, N6-cyclohexyladenosine, on 22Na uptake by rat brain synaptosomes stimulated by veratridine was investigated. In the presence of N6-cyclohexyladenosine, both the initial rate and the maximum sodium uptake were decreased. The inhibitory effect of N6-cyclohexyladenosine on sodium uptake by synaptosomes after 5 s of incubation with 22Na was concentration-dependent, antagonized by 1,3-dipropyl-8-p-sulfophenylxanthine, and attenuated by increasing the concentration of veratridine. The possibility that the adenosine analogue, by activating a xanthine-sensitive adenosine receptor, can operate inhibition of the voltage-dependent sodium channels is discussed.
Simões
A P
AP
Laboratory of Pharmacology, Gulbenkian Institute of Science, Oeiras, Portugal.
Oliveira
P C
PC
Sebastião
A M
AM
Ribeiro
J A
JA
D000241:Adenosine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D009994:Osmolar Concentration; D058914:Purinergic Antagonists; D051381:Rats; D012964:Sodium / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics*; D012979:Sodium Radioisotopes; D013574:Synaptosomes / Q000378:metabolism*; D013997:Time Factors; D014701:Veratridine / Q000494:pharmacology*; D014702:Veratrine / Q000031:analogs & derivatives*; D014970:Xanthines / Q000494:pharmacology
D058914:Purinergic Antagonists; D012979:Sodium Radioisotopes; D014970:Xanthines; C027513:N(6)-cyclohexyladenosine; D014701:Veratridine; C053896:1,3-dipropyl-8-(4-sulfophenyl)xanthine; D012964:Sodium; D014702:Veratrine; D000241:Adenosine