Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties.
Arachidonate 15-lipoxygenase was purified from human eosinophil-enriched leukocytes after showing that 15-lipoxygenase activity was 100-fold greater in eosinophils than in neutrophils. Partial purification was achieved using ammonium sulfate precipitation, cation-exchange and hydrophobic-interaction chromatography. New evidence is presented suggesting that 15-lipoxygenase has electrostatic and hydrophobic properties distinct from 5-lipoxygenase. In addition, ATP is shown to inhibit, and phosphatidylcholine is shown to stimulate, 15-lipoxygenase, suggesting a regulatory role for these compounds in the lipoxygenation of arachidonic acid.
Sigal
E
E
Department of Medicine, University of California Medical Center, San Francisco 94143.
Grunberger
D
D
Cashman
J R
JR
Craik
C S
CS
Caughey
G H
GH
Nadel
J A
JA
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001093:Arachidonate 15-Lipoxygenase / Q000097:blood*; D001094:Arachidonate 5-Lipoxygenase / Q000097:blood; D001091:Arachidonate Lipoxygenases / Q000097:blood*; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D004804:Eosinophils / Q000201:enzymology*; D005590:Fractional Precipitation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D007962:Leukocytes / Q000201:enzymology*; D016859:Lipoxygenase Inhibitors; D009504:Neutrophils / Q000201:enzymology; D010713:Phosphatidylcholines / Q000494:pharmacology
D006893:Hydroxyeicosatetraenoic Acids; D016859:Lipoxygenase Inhibitors; D010713:Phosphatidylcholines; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D000255:Adenosine Triphosphate; D001091:Arachidonate Lipoxygenases; D001093:Arachidonate 15-Lipoxygenase; D001094:Arachidonate 5-Lipoxygenase