Hepatic lipid peroxidation in CoCl2-treated rats, evidenced by elevated concentrations of thiobarbituric acid chromogens.
Parenteral administration of cobalt chloride (CoCl2) to male Fischer-344 rats caused hepatic lipid peroxidation, as evidenced by the thiobarbituric acid (TBA) reaction for malondialdehyde (MDA) and related chromogens in fresh liver homogenates. After a single sc injection of CoCl2 (300 mumol/kg, body wt.), hepatic concentrations of TBA-chromogens became significantly increased by 4 h and reached peak levels at 24-72 h post-injection. For example, in rats killed 48 h post-injection, hepatic TBA-chromogens (mean +/- SD, N = 7) averaged 607 +/- 141 nmol/g of tissue, wet wt., (P less than 0.01 versus 245 +/- 73 nmol/g in 19 controls). A dose-effect relationship was observed between CoCl2 dosages (100 to 450 mumol/kg, body wt.) and hepatic concentrations of TBA-chromogens in rats killed 24 h post-injection. This study indicates that hepatic lipid peroxidation develops as a consequence of acute CoCl2 toxicity in rats.
Sunderman
F W
FW
Department of Laboratory Medicine, University of Connecticut Medical School, Farmington 06032.
Zaharia
O
O
D000818:Animals; D002863:Chromogenic Compounds; D003035:Cobalt / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D013858:Thiobarbiturates
D002863:Chromogenic Compounds; D008054:Lipid Peroxides; D013858:Thiobarbiturates; D003035:Cobalt; C018021:cobaltous chloride; C029684:thiobarbituric acid