Calibration of thin-layer chromatography with flame ionization detection for the analysis of natural lipid samples.
Thin-layer chromatography coupled with flame ionization detection has been used to develop a method to quantitate fish lipids. Quantitation of fractionated lipid classes is usually accomplished through calibration with standards. Our work shows that various standards within a class, such as triglycerides or free fatty acids give substantially different responses to the detector. A method has been developed in which a composite sample of salmon lipid is prepared with an internal standard. This technique eliminates the variable detector response observed for compounds within a class and provides more accurate quantitation of oils from which the calibration samples were prepared. The lipid classes quantitated were triglycerides, free fatty acids, phosphatidylethanolamine and phosphatidylcholine.
Whitsett
J F
JF
Department of Chemistry, University of Alaska-Anchorage 99508.
Kennish
J M
JM
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer / Q000592:standards; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D009132:Muscles / Q000032:analysis; D012474:Salmon / Q000378:metabolism
D005227:Fatty Acids; D008055:Lipids