Two different adenylyl cyclases in brain distinguished by monoclonal antibodies.
Four monoclonal antibodies, raised against the 115-kDa adenylyl cyclase from bovine brain [Pfeuffer, E. et al. (1985) EMBO J. 4, 3675-3679] have been selected and designated BBC-1 to BBC-4. BBC-1 and BBC-3 are highly specific for the 115-kDa enzyme from bovine brain. The two other antibodies, BBC-2 and BBC-4, recognize an additional 150-kDa adenylyl cyclase in bovine brain, but also in brain tissue from other species. In membranes from lung and myocardium (bovine and rabbit) only the 150-kDa species is detected by the crossreacting antibodies BBC-2 and BBC-4. The two adenylyl cyclases from brain can be separated by calmodulin-Sepharose: only the enzyme of 115 kDa but not that of 150 kDa was retained by the affinity resin and could be stimulated by Ca2+/calmodulin. The data obtained with these antibodies of defined specificity provide for the first time direct evidence for the presence of two distinct adenylyl cyclase species in brain tissue.
Mollner
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S
Physiologisch-Chemisches Institut der Universität Würzburg, Federal Republic of Germany.
Pfeuffer
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T
D000262:Adenylyl Cyclases / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001921:Brain / Q000201:enzymology*; D002118:Calcium / Q000502:physiology; D002417:Cattle; D003429:Cross Reactions; D008168:Lung / Q000201:enzymology; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology; D011817:Rabbits; D013045:Species Specificity; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000262:Adenylyl Cyclases; D002118:Calcium