title: Immunohistochemical localization of creatine kinase BB in primary breast cancer: correlation with estrogen receptor content.
abstract: The brain-type (BB) isoenzyme of creatine kinase (CK) has recently been shown to be estrogen-regulated in human breast cancer cells in vitro. In this study we have correlated the presence of CK-BB, evaluated by immunoperoxidase procedures, with the estrogen receptor (ER) content in 35 primary breast cancers. Of 35 tumors examined, 66% revealed moderate to strong CK-BB immunoreactivity. Staining was exclusively located in the cytoplasm of neoplastic cells. A positive relationship was observed between CK-BB positivity and ER content with ER-rich tumors (greater than 50 fmoles/mg protein) showing a more intense immunoreactivity than ER-poor ones. The results indicate that CK-BB is estrogen-regulated in human breast cancer and suggest that evaluation of this enzyme may be of potential value for the detection of hormone responsive tumors.
authors.affiliation: Istituto di Clinica Ginecologica e Ostetrica, Università Cattolica, Roma, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Scambia
authors.forename: G
authors.initials: G
authors.lastname: Santeusanio
authors.forename: P B
authors.initials: PB
authors.lastname: Panici
authors.forename: S
authors.initials: S
authors.lastname: Iacobelli
authors.forename: S
authors.initials: S
authors.lastname: Mancuso
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000032:analysis*; D003402:Creatine Kinase / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes; D011960:Receptors, Estrogen / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D007527:Isoenzymes; D011960:Receptors, Estrogen; D003402:Creatine Kinase