Cell-mediated responses and protection elicited by a carbohydrate-lipid-containing fraction extracted from Leishmania major promastigotes.
Carbohydrate-lipid-containing fractions (CLF) extracted from Leishmania major promastigotes and recognized by sera from immune but not from normal human donors were evaluated for their capacity to elicit cell-mediated responses. It was found that one of these fractions, CLF-1, stimulated the in vitro response of lymphocytes from immune but not from normal human donors. A similarly extracted fraction from L. donovani parasites also elicited an in vitro response by cells from donors immune to L. major. The response was mediated by antigen-presenting cells, and specific Leu 3+ Leu 2- T cells from a human T-cell line responded to the antigen. In vivo, the CLF-1 elicited delayed-type hypersensitivity (DTH) response in L. major-immunized C3H mice, which was comparable to the DTH response elicited by freeze-thawed and sonicated L. major promastigotes. C3H mice were vaccinated with CLF-1 prior to challenge with live L. major promastigotes. Mice vaccinated with CLF-1-containing liposomes showed a significant degree of protection to challenge. These results suggest that the carbohydrate-lipid-containing fraction described here may represent a functional antigenic entity from Leishmania parasites.
Frankenburg
S
S
Department of Parasitology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Rosen
G
G
Londner
M V
MV
D000818:Animals; D006001:Glycoconjugates / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D007111:Immunity, Cellular; D007114:Immunization; D007893:Leishmania donovani / Q000276:immunology; D007895:Leishmania tropica / Q000276:immunology*; D008081:Liposomes; D008213:Lymphocyte Activation; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000276:immunology*; D014611:Vaccination
D006001:Glycoconjugates; D006017:Glycolipids; D006023:Glycoproteins; D008081:Liposomes; D011134:Polysaccharides