Deposition of endothelial fibronectin on polymeric surfaces.
Cellular fibronectin is deposited on tissue culture polystyrene during the adhesion and spreading of cultured human endothelial cells (HEC). Following the seeding of HEC upon this polymer, larger amounts of fibronectin are deposited as both cell density and incubation time increase. Our results indicate that the ability to deposit cellular fibronectin onto a polymeric surface is a condition for the spreading and proliferation of HEC.
van Wachem
P B
PB
Department of Chemical Technology, Twente University, Enschede, The Netherlands.
Beugeling
T
T
Mallens
B W
BW
Dekker
A
A
Feijen
J
J
Bantjes
A
A
van Aken
W G
WG
D000327:Adsorption; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011108:Polymers; D011137:Polystyrenes; D014471:Umbilical Veins
D001672:Biocompatible Materials; D003470:Culture Media; D005353:Fibronectins; D011108:Polymers; D011137:Polystyrenes