Mapping of the Saccharomyces cerevisiae CDC3, CDC25, and CDC42 genes to chromosome XII by chromosome blotting and tetrad analysis.
CDC3, CDC25 and CDC42 were localized to chromosome XII by hybridizing the cloned genes to Southern blots of chromosomes separated by orthogonal-field-alternation gel electrophoresis. Meiotic tetrad analyses further localized these genes to the region distal to the RDN1 locus on the right arm of the chromosome. The STE11 gene, which had previously been mapped to chromosome XII (Chaleff and Tatchell, 1985), was found to be tightly linked to ILV5. The data suggest a map order of CEN12-RDN1-CDC42-(CDC25-CDC3)-(ILV5- STE11)-URA4. Certain oddities of the data set raise the possibility that there may be constraints on the patterns of recombination in this region of chromosome XII.
Johnson
D I
DI
Department of Biology, University of Michigan, Ann Arbor 48109.
Jacobs
C W
CW
Pringle
J R
JR
Robinson
L C
LC
Carle
G F
GF
Olson
M V
MV
D015139:Blotting, Southern; D002453:Cell Cycle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D008040:Genetic Linkage; D009693:Nucleic Acid Hybridization; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D015342:DNA Probes; D004271:DNA, Fungal