Neuraminidase fluorescence test for the differentiation of Trypanosoma cruzi and Trypanosoma rangeli.
The cell free supernatants of 13 stocks of Trypanosoma rangeli/T. rangeli-like, 50 stocks of T. cruzi/T. cruzi-like and 3 stocks of T. conorhini grown in culture were examined by using the 2-(4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid) test. It could be demonstrated that only the supernatants of the T. rangeli/T. rangeli-like stocks showed a white-yellow fluorescence under UV light. In contrast, the cell free supernatants of the T. cruzi/T. cruzi-like and T. conorhini stocks showed an ash-grey colour under UV light undistinguishable from that of the control reactions. The 4-MUAc test could be a method of choice for the routine detection of T. rangeli/T. rangeli-like flagellates in culture.
Schottelius
J
J
Department of Protozoology, Bernhard-Nocht-Institute for Nautical and Tropical Medicine, Hamburg, Federal Republic of Germany.
D000818:Animals; D006801:Humans; D009439:Neuraminidase / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014345:Trypanosoma / Q000201:enzymology / Q000302:isolation & purification*; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000302:isolation & purification*; D014466:Ultraviolet Rays
D009439:Neuraminidase