The turnover number for band 3-mediated sulfate transport in phosphatidylcholine bilayers.
The anion transport system of the human erythrocyte membrane was reconstituted in unilamellar phosphatidylcholine vesicles, and a vesicle subpopulation of a narrow size distribution was isolated from the sample by gel filtration. In this subpopulation, the turnover number of the transport protein (the band 3 protein) for sulfate transport was determined. It was found that, in the reconstituted system, the protein transports sulfate 5-10 times faster than in the human erythrocyte membrane.
Scheuring
U
U
Max-Planck-Institut für Biophysik, Frankfurt am Main, FRG.
Lindenthal
S
S
Grieshaber
G
G
Haase
W
W
Schubert
D
D
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D000838:Anions; D001692:Biological Transport; D002850:Chromatography, Gel; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008854:Microscopy, Electron; D010713:Phosphatidylcholines / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*
D001457:Anion Exchange Protein 1, Erythrocyte; D000838:Anions; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D013431:Sulfates