title: Characterization of the protein product of bcl-2, the gene involved in human follicular lymphoma.
abstract: A protein corresponding in size to the bcl-2 alpha protein obtained after in vitro transcription and in vitro translation in a rabbit reticulocyte lysate was specifically immunoprecipitated from a human B-cell extract using rabbit polyclonal antiserum raised against a beta-galactosidase/bcl-2 fusion protein. Peptide mapping with V8 protease confirmed the identity of this protein as human bcl-2 alpha. Subcellular fractionation of cellular protein followed by immunoprecipitation showed that the bcl-2 alpha protein is associated with the cell membrane.
authors.affiliation: Wistar Institute, Philadelphia 19104.
authors.forename: Y
authors.initials: Y
authors.lastname: Tsujimoto
authors.forename: N
authors.initials: N
authors.lastname: Ikegaki
authors.forename: C M
authors.initials: CM
authors.lastname: Croce
mesh_terms: D001402:B-Lymphocytes / Q000502:physiology*; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics*; D005796:Genes; D006801:Humans; D007158:Immunologic Techniques; D008223:Lymphoma / Q000235:genetics*; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D010449:Peptide Mapping; D011518:Proto-Oncogene Proteins / Q000302:isolation & purification*; D019253:Proto-Oncogene Proteins c-bcl-2; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014178:Translocation, Genetic
chemical_list: D004273:DNA, Neoplasm; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011993:Recombinant Fusion Proteins; C052617:Bcl2 protein, mouse