Protective value of the plasmid-coded outer membrane protein of enteroinvasive Escherichia coli.
O-antigenically not related enteroinvasive Escherichia coli strains and rabbit sera prepared with them were used to study the role of plasmid-coded outer membrane proteins in protective immunity. Active immunization experiments were performed using a mouse model based on a long-lasting symptomless carriership after elimination of the bowel flora by streptomycin. Preliminary histological studies showed adhesion, penetration, intraepithelial multiplication, and epithelial desquamation after infection. In active immunization experiments only massive oral doses evoked protective immunity. Seroconversion against the plasmid-coded antigens was not observed in mice. Passive immunization was carried out in chick embryos with unabsorbed sera. A high level of protectivity was reached by serogroup-specific sera and a very low but significant protection was yielded by antibodies against the plasmid-coded protein antigens.
Kétyi
I
I
Institute of Microbiology, University Medical School, Pécs, Hungary.
Pál
T
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002642:Chick Embryo; D004195:Disease Models, Animal; D004405:Dysentery, Bacillary / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D004751:Enteritis / Q000276:immunology / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D007110:Immunity, Active; D007116:Immunization, Passive; D051379:Mice; D010957:Plasmids; D014774:Virulence
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins