Klebsiella pneumoniae C3 lipopolysaccharide mutants obtained by resistance to bacteriophage FC3-9.
Bacteriophage FC3-9 is one of the several bacteriophages of Klebsiella pneumoniae C3 isolated in our laboratory. Mutants resistant to this bacteriophage were isolated and found to be devoid of lipopolysaccharide O antigen, modified in outer-membrane protein composition and sensitive to complement killing (serum-sensitive), unlike the wild-type strain. Serum-resistant mutants were isolated from these strains. They regained the lipopolysaccharide O antigen and the wild-type outer membrane protein composition and became sensitive to bacteriophage FC3-9.
Benedí
V J
VJ
Departamento de Microbiología Facultad de Biología, Universidad de Barcelona, Spain.
Jofre
J
J
Tomás
J M
JM
D000942:Antigens, Bacterial / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001435:Bacteriophages / Q000276:immunology*; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D008070:Lipopolysaccharides / Q000235:genetics* / Q000276:immunology; D009154:Mutation
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides