A high-speed liquid-chromatographic method for measuring urinary porphyrins.
We describe a rapid quantitative and qualitative "high-performance" liquid-chromatographic (HPLC) method for measuring porphyrins in urine. Direct injection of acidified, filtered urine onto a 3-micron (particle size) 3-cm-long reversed-phase column fully resolves uroporphyrin, hepta-, hexa-, and pentacarboxylic acid porphyrins, and coproporphyrin. Instrument response is linearly related to concentration over the range 25 to 300 nmol/L. The method provides data essential for the differential diagnosis of porphyric states, including porphyria variegata and porphyria cutanea tarda. This relatively inexpensive method requires a run time of only 8 min per sample, making it particularly suitable for routine use in the clinical chemistry laboratory.
Johnson
P M
PM
Division of Biochemistry, Ottawa Civic Hospital, Ontario, Canada.
Perkins
S L
SL
Kennedy
S W
SW
D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000652:urine; D005420:Flavoproteins; D006801:Humans; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000172:deficiency; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011164:Porphyrias / Q000652:urine*; D011166:Porphyrins / Q000652:urine*; D050768:Protoporphyrinogen Oxidase; D012871:Skin Diseases / Q000652:urine; D014575:Uroporphyrinogen Decarboxylase / Q000172:deficiency; D014578:Uroporphyrins / Q000652:urine
D003306:Coproporphyrins; D005420:Flavoproteins; D024101:Mitochondrial Proteins; D011166:Porphyrins; D014578:Uroporphyrins; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C493978:PPOX protein, human; D050768:Protoporphyrinogen Oxidase; D014575:Uroporphyrinogen Decarboxylase