title: Hepatic handling of a synthetic gamma-labeled bile acid (75SeHCAT).
abstract: 75Se-homocholic acid-taurine (75SeHCAT) is the first available gamma-labeled bile acid, and should therefore be handled more efficiently and specifically by the liver than previous hepatoscintigraphic agents. We have measured serum and hepatic kinetics for 75SeHCAT, and compared them with those for the conventional hepatobiliary scintigraphic agent 99mTc-hepatoiminodiacetic acid, and with serum kinetics for the corresponding natural bile acid, [14C]cholic acid-taurine. We used a dynamic scintigraphic technique and serial blood sampling in 8 subjects. Initial hepatic uptake rate was identical to initial serum disappearance rate (14% dose/min) for 75SeHCAT, but significantly lower for 99mTc-hepatoiminodiacetic acid (6% vs. 14% dose/min, p less than 0.001). Hepatic transit time was shorter for 75SeHCAT (13 min vs. 22 min, p less than 0.02), net hepatic excretory rate was more rapid (1.4% vs. 0.8% dose/min, p less than 0.001), and urinary excretion was lower (1.0% vs. 9.0% dose, p less than 0.001). Initial and late-plasma disappearance rates were significantly lower for 75SeHCAT (14.3% and 1.5% dose/min) than for [14C]cholic acid-taurine (21.3% and 2.8% dose/min, respectively), and plasma clearance was also lower (275 vs. 670 ml/min). In vitro, 75SeHCAT was bound to serum proteins more completely than [14C]cholic acid-taurine (90.4% vs. 86.5%, p less than 0.005). We conclude that 75SeHCAT provides a hepatoscintigraphic agent that is handled more efficiently and specifically by the liver than the conventionally used agent 99mTc-hepatoiminodiacetic acid. It is not cleared from the serum as rapidly as [14C]cholic acid-taurine, probably due to its stronger protein binding. The clinical value of 75SeHCAT in assessing liver disease should be investigated.
authors.affiliation: Department of Medicine, St. George's Hospital Medical School, London, United Kingdom.
authors.forename: G
authors.initials: G
authors.lastname: Galatola
authors.forename: R P
authors.initials: RP
authors.lastname: Jazrawi
authors.forename: C
authors.initials: C
authors.lastname: Bridges
authors.forename: A E
authors.initials: AE
authors.lastname: Joseph
authors.forename: T C
authors.initials: TC
authors.lastname: Northfield
mesh_terms: D000328:Adult; D000368:Aged; D002250:Carbon Radioisotopes; D005260:Female; D006801:Humans; D007098:Imino Acids / Q000097:blood / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009942:Organometallic Compounds / Q000097:blood / Q000493:pharmacokinetics; D013656:Taurocholic Acid / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D019780:Technetium Tc 99m Lidofenin
chemical_list: D002250:Carbon Radioisotopes; D007098:Imino Acids; D009942:Organometallic Compounds; D013656:Taurocholic Acid; D019780:Technetium Tc 99m Lidofenin; C033638:23-seleno-25-homotaurocholic acid