Composition of proteoglycans synthesized by rabbit aortic explants in culture and the effect of experimental atherosclerosis.
The synthesis of proteoglycans by aorta explants from rabbits with diet-induced atherosclerosis and controls was studied by 35S-incorporation. Proteoglycans were isolated under dissociative conditions from incubation medium and from arterial explants. Additionally, the tissue proteoglycans that were not extracted by 4 M guanidine-HCl were solubilized by digestion of the tissue by elastase in the presence of proteinase inhibitors. The residual tissue was hydrolyzed by papain and glycosaminoglycans were isolated. The atherosclerotic aorta tissue incorporated twice the amount of 35S into proteoglycans than observed for controls; in both groups about 70% of the label incorporated into the tissue was noted in the proteoglycans extracted by guanidine-HC;, while about 30% of the total 35S-labeled proteoglycans synthesized by the explants were found in the media. Atherosclerotic tissue incorporated 35S predominantly into chondroitin sulfate proteoglycans when compared to control tissue. The chondroitinase ABC-digestable proteoglycans that were extracted by guanidine-HCl from atherosclerotic tissues were of larger molecular size than those from control tissue, but the core proteins from these preparations were similar. The heparan sulfate proteoglycan that was obtained by dissociative extraction from atherosclerotic tissue had greater amounts of N-acetyl and lesser amounts of N-sulfate ester groups than the preparation from control tissue. Digestion of the tissue by elastase yielded heparan sulfate proteoglycan as the major constituent in both groups, although atherosclerotic tissue contained relatively small amounts of this proteoglycan. The residual tissue from both groups contained chondroitin sulfate and heparan sulfate as the major glycosaminoglycans with the latter showing a decrease with atherosclerosis. Atherosclerotic tissue secreted into the medium about two-fold more 35S-labeled proteoglycans with larger molecular size than control tissue; proteoglycans of the heparan sulfate and chondroitin sulfate types were the major constituents in the culture medium of both tissues. Thus, proteoglycans undergo both quantitative and qualitative changes in atherosclerosis, reflecting the enhanced smooth muscle cell activity. These changes are potentially important in modulating lipoprotein binding and hemostatic properties, as well as fibrillogenesis of the arterial wall.
Radhakrishnamurthy
B
B
Department of Medicine, Louisiana State University Medical Center, New Orleans 70112.
Srinivasan
S R
SR
Eberle
K
K
Ruiz
H
H
Dalferes
E R
ER
Sharma
C
C
Berenson
G S
GS
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism*; D002850:Chromatography, Gel; D004036:Diet, Atherogenic; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000302:isolation & purification; D019791:Guanidine; D006146:Guanidines; D008297:Male; D009924:Organ Culture Techniques; D010196:Pancreatic Elastase; D011509:Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D011817:Rabbits; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes
D006025:Glycosaminoglycans; D006146:Guanidines; D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D010196:Pancreatic Elastase; D019791:Guanidine