Cytotoxic and mutagenic effects of emodin on cultured mouse carcinoma FM3A cells.
Employing a suspension culture of a mouse mammary carcinoma cell line, FM3A cells, the cytotoxicity and induced mutagenicity of emodin (EM) were examined and compared with those of 2-hydroxy-emodin (2-OH-EM), which was identified as an active form of EM in the Ames/microsomes assay. EM was cytotoxic to FM3A cells in concentrations of 1-10 micrograms/ml, and induced 6-thioguanine-resistant (6TGr) mutation. 2-OH-EM was a little more toxic than EM, but induced little mutation.
Morita
H
H
Kihara Institute for Biological Research, Yokohama City University, Japan.
Umeda
M
M
Masuda
T
T
Ueno
Y
Y
D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004642:Emodin / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D009152:Mutagenicity Tests; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000880:Anthraquinones; D004642:Emodin