Enzyme-linked immunosorbent assay for the detection of antibodies to the alcelaphine herpesvirus of malignant catarrhal fever in exotic ruminants.
An ELISA for antibodies to the alcelaphine herpesvirus-1 of malignant catarrhal fever was developed. Of sera that represented 42 exotic ruminant species, 216 were evaluated by the ELISA and a virus-neutralization test. A significant correlation (r = 0.564, P less than 0.001, n = 216) between the ELISA and virus-neutralization test results was found. Of the sera having positive test results by virus neutralization, 86.1% also had positive results by the ELISA, and of the sera having negative test results by virus neutralization, 83.9% also had negative results by the ELISA. The presence of antibody, as measured by the ELISA, correlated with clinical signs of malignant catarrhal fever and the isolation of herpesvirus.
Wan
S K
SK
Oklahoma Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
Castro
A E
AE
Heuschele
W P
WP
Ramsay
E C
EC
D000818:Animals; D000836:Animals, Zoo / Q000276:immunology*; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D003670:Deer / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006564:Herpesviridae / Q000276:immunology*; D008304:Malignant Catarrh / Q000175:diagnosis* / Q000276:immunology; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D012418:Ruminants / Q000276:immunology*
D000914:Antibodies, Viral