Streptococcus sanguis surface antigens and their interactions with saliva.
Saliva-binding molecules of Streptococcus sanguis and their receptors were investigated. Streptococcal cell surfaces were extracted with a barbital buffer and examined immunochemically. Strains G9B and Blackburn, which adhere specifically to saliva-coated hydroxyapatite via immunologically related adhesins, possess 80-, 62-, and 52-kilodalton (kDa), and 52-, 42-, and 29-kDa polypeptides, respectively, which correlate with adhesion to saliva-coated hydroxyapatite. Nonadherent strains Adh- and M-5 lack these antigens. In an immunoblot overlay, the putative adhesins bound to a 73-kDa receptor present in submandibular saliva but not in parotid saliva. G9B also contains a 160-kDa surface protein which bound to an unidentified receptor in both submandibular and parotid saliva samples. Blackburn barbital-extracted components bound to 78- and 70-kDa receptors in parotid saliva. These bacterial-salivary interactions may be important in the regulation of oral ecology.
Lamont
R J
RJ
Department of Microbiology, Dental School, University of Pennsylvania, Philadelphia 19104.
Rosan
B
B
Murphy
G M
GM
Baker
C T
CT
D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000032:analysis*; D001462:Barbital; D006801:Humans; D007118:Immunoassay; D008565:Membrane Proteins / Q000032:analysis; D011485:Protein Binding; D012463:Saliva / Q000378:metabolism / Q000382:microbiology*; D013298:Streptococcus sanguis / Q000276:immunology* / Q000378:metabolism
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins; C045564:saliva-interacting cell wall protein, Streptococcus; D001462:Barbital