[Dependence of the proliferation of cell line 3T6 on a totally serum-free medium on epidermal growth factor].
The Eagle medium containing the epidermal growth factor (EGF), insulin, transferrin and a source of iron is able to support proliferation of 3T6 cells in the absence of serum. The formation of 3T6 cell clones in the similar medium supplemented with non-essential amino acids confirms the usefulness of such a medium for cell cultivation. It has been found that the exclusion of EGF rather than other above components from the medium inhibits most highly the increase in cell number.
Gudkova
D A
DA
Sorokin
A B
AB
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007106:Immune Sera / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D013997:Time Factors; D014168:Transferrin / Q000494:pharmacology
D000596:Amino Acids; D003470:Culture Media; D007106:Immune Sera; D007328:Insulin; D014168:Transferrin; D004815:Epidermal Growth Factor; D007501:Iron
Zavisimost' proliferatsii kletok linii 3T6 v pol'nost'iu bessyvorotochnoĭ srede ot épidermal'nogo faktora rosta.