Pertussis toxin inhibits angiotensin II-mediated phosphatidylinositol breakdown and ADP-ribosylates a 40 Kd protein in cultured smooth muscle cells.
Angiotensin II causes an increase of inositol phosphate production in cultured vascular smooth muscle cells from rat aorta. Pretreatment of the cells with pertussis toxin attenuates this effect. Pertussis toxin ADP-ribosylates a protein of about 40 kD in a crude membrane fraction. These data demonstrate the possible involvement of a GTP-binding protein (G-protein) in the angiotensin II-induced activation of phosphoinositidase in vascular smooth muscle cells.
Gaul
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Gödecke Research Institute, Freiburg, FRG.
Gierschik
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P
Marmé
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D
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D007294:Inositol / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D010716:Phosphatidylinositols; D011506:Proteins; D010566:Virulence Factors, Bordetella; D000804:Angiotensin II; D000246:Adenosine Diphosphate Ribose; D007294:Inositol; D037342:Pertussis Toxin