Distribution of chromogranin A and secretogranin I (chromogranin B) in neuroendocrine cells and tumors.
The distribution of chromogranin A and secretogranin I (chromogranin B) in normal and neoplastic human endocrine tissues was analyzed with two human monoclonal antibodies against chromogranin A, anti-bovine antiserum against chromogranin A, and an anti-rat antiserum against secretogranin I. Western blotting analyses showed both chromogranin A and secretogranin I in normal adrenals, pheochromocytomas, a pituitary adenoma, and in normal pituitary glands, but not in a bladder carcinoma. Rat adrenal medullary and anterior pituitary tissues reacted with the polyclonal chromogranin A and secretogranin I antisera, but not with the two monoclonal chromogranin A antibodies. All antibodies reacted with most of the neuroendocrine cells and tumors examined. Pituitary prolactinomas contained immunoreactive secretogranin I, but not chromogranin A. Analysis of the distribution of chromogranin A and secretogranin I in pancreatic islet cells showed that chromogranin A was found predominantly in the glucagon-producing A cells, whereas secretogranin I was present in less than 5% of islet cells. These results indicate that chromogranin A and secretogranin I are both useful in the characterization of some neuroendocrine cells and neoplasms.
Lloyd
R V
RV
Department of Pathology, University of Michigan, Ann Arbor 48109.
Cano
M
M
Rosa
P
P
Hille
A
A
Huttner
W B
WB
D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D053379:Chromogranin A; D053378:Chromogranin B; D002864:Chromogranins / Q000032:analysis*; D004702:Endocrine Glands / Q000032:analysis* / Q000166:cytology; D004700:Endocrine System Diseases / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D009369:Neoplasms / Q000032:analysis* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000032:analysis; D010911:Pituitary Neoplasms / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013964:Thyroid Neoplasms / Q000032:analysis
D000911:Antibodies, Monoclonal; C506150:CHGA protein, human; C506134:CHGB protein, human; D053379:Chromogranin A; D053378:Chromogranin B; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D011506:Proteins; C506153:chromogranin A, rat; C506140:chromogranin B, rat