Occurrence of glucose polymer in undifferentiated PC12 cells.
A large glucose polymer was found, following pronase digestion, in PC12 pheochromocytoma cells metabolically labeled with [1-3H]galactose. The polymer was excluded from a Bio-Gel A-0.5 m column and adsorbed by immobilized concanavalin A-Sepharose from which it was eluted with 10 mM alpha-methylmannoside. Glucose was found to be the sole component monosaccharide. Except for those capable of degrading glycogen, no exo- or endo-glycosidases cleaved the polymer. This is the first report on the occurrence of a glucose polymer in undifferentiated PC12 cells.
Rasilo
M L
ML
Laboratory of Glycoconjugate Research, Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Yamagata
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D000310:Adrenal Gland Neoplasms / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D005690:Galactose / Q000378:metabolism; D005936:Glucans / Q000032:analysis* / Q000096:biosynthesis; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D006003:Glycogen / Q000032:analysis; D008358:Mannose / Q000378:metabolism; D010673:Pheochromocytoma / Q000032:analysis*; D051381:Rats; D014316:Tritium
D005936:Glucans; D014316:Tritium; D006003:Glycogen; D005947:Glucose; D005944:Glucosamine; D008358:Mannose; D005690:Galactose