Partial characterization of a soluble mitogenic factor from medulloblastoma.
To determine how medulloblastoma cells might influence the proliferation and phenotype of normal stromal cells, normal human leptomeningeal cells were treated in culture with medulloblastoma-conditioned medium; their ability to incorporate tritiated thymidine and synthesize collagen was measured. The treated leptomeningeal cells had a significantly greater uptake of tritiated thymidine and grew faster than control leptomeningeal cells. Immunofluorescence studies demonstrated a greater intensity of staining for procollagen type III in the cell layer of the treated cultures than in control cultures; diethylaminoethyl (DEAE)-cellulose chromatography of the medium showed that the treated cells synthesized predominantly type III collagen, whereas control cells synthesized type I collagen. Analysis of the medulloblastoma-conditioned medium revealed that the soluble factor responsible for these effects in an acid- and heat-stable protein. The increased proliferation and altered collagen synthesis induced in leptomeningeal cell cultures by a soluble factor from a medulloblastoma are examples of how tumor and stromal elements interact, and may be related to the process of desmoplasia often observed in medulloblastomas in vivo.
Rutka
J T
JT
Department of Neurological Surgery, School of Medicine, University of California, San Francisco.
Hall
J
J
Giblin
J R
JR
Dougherty
D V
DV
Edwards
M S
MS
Stern
R
R
Rosenblum
M L
ML
D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D003094:Collagen / Q000096:biosynthesis*; D003470:Culture Media; D005260:Female; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D008297:Male; D008527:Medulloblastoma / Q000503:physiopathology*; D008578:Meninges / Q000166:cytology / Q000378:metabolism / Q000503:physiopathology*; D008875:Middle Aged
D003470:Culture Media; D006133:Growth Substances; D003094:Collagen