title: Simultaneous determination of chloroquine and metronidazole in human biological fluid by high pressure liquid chromatography.
abstract: Chloroquine (CQ) and metronidazole (MZ) were measured in human urine and plasma by HPLC with UV detection. This method was used to analyse plasma levels in 4 African volunteers after an oral dose of 1000 mg CQ and 750 mg MZ, in a European on weekly prophylaxis of 500 mg CQ, and on 50 hospital urine samples. In the Africans peak plasma levels were over 1 microgram/ml and peak time was 1 1/2-2 hr. In the European plasma levels ranged from 0.58 to 0.36 microgram/ml. Over 80% of the urine samples contained CQ, MZ or both. The assay system was found flexible and economical for the therapeutic monitoring of these two important tropical drugs.
authors.affiliation: Department of Pharmacology and Therapeutics, College of Medicine, University of Nigeria Enugu Campus.
authors.forename: P O
authors.initials: PO
authors.lastname: Okonkwo
authors.forename: E I
authors.initials: EI
authors.lastname: Eta
mesh_terms: D000328:Adult; D002738:Chloroquine / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000032:analysis* / Q000097:blood / Q000652:urine; D009549:Nigeria
chemical_list: D008795:Metronidazole; D002738:Chloroquine