Dynamic continuity of cytoplasmic and membrane compartments between plant cells.
Fluorescence photobleaching was employed to examine the intercellular movement of fluorescein and carboxyfluorescein between contiguous soybean root cells (SB-1 cell line) growing in tissue culture. Results of these experiments demonstrated movement of these fluorescent probes between cytoplasmic (symplastic) compartments. This symplastic transport was inhibited with Ca2+ in the presence of ionophore A23187, and also with the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Both of these agents have previously been demonstrated to inhibit gap junction-mediated cell-cell communication in animal cells. In a companion experiment, a fluorescent phospholipid analogue, N-4-nitrobenzo-2-oxa-1,3-diazole phosphatidylcholine (NBD-PC), was incorporated into soybean cell membranes to examine whether dynamic membrane continuity existed between contacting cells, a transport route not existing between animal cells. Photobleaching single soybean cells growing in a filamentous strand demonstrated that phospholipid did exchange between contiguous cells.
Baron-Epel
O
O
Department of Biochemistry, Michigan State University, East Lansing 48824.
Hernandez
D
D
Jiang
L W
LW
Meiners
S
S
Schindler
M
M
D002118:Calcium / Q000494:pharmacology; D002450:Cell Communication; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D007834:Lasers; D008856:Microscopy, Fluorescence; D059828:Plant Cells; D010944:Plants / Q000648:ultrastructure; D013025:Glycine max; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D005452:Fluoresceins; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium