title: Immunohistochemical localization of glutamate dehydrogenase in rat liver: plasticity of distribution during development and with hormone treatment.
abstract: In adult rat liver, glutamate dehydrogenase is present in high concentrations around the terminal portal (zone 1) and hepatic (zone 3) veins, whereas its concentration is low in the intermediate zone. Although the size and staining intensity of the periportal glutamate dehydrogenase-positive compartment are less than those of the pericentral compartment, it can expand under appropriate endocrine conditions, leading to a homogeneous distribution. At birth, glutamate dehydrogenase is also homogeneously distributed. Glutamate dehydrogenase disappears from the periportal compartment during the first postnatal week and reappears in that compartment after weaning. These observations indicate an independent regulation of glutamate dehydrogenase levels in the periportal and pericentral zone. The size of the periportal glutamate dehydrogenase-containing zone is appreciably smaller than that of carbamoylphosphate synthetase, whereas the pericentral glutamate dehydrogenase-containing zone is appreciably larger than that of glutamine synthetase. The heterogeneous distribution of glutamate dehydrogenase suggests the possibility that, under normal conditions, deamination of glutamate prevails in the periportal compartment and amination of glutamate in the pericentral compartment.
authors.affiliation: Department of Anatomy and Embryology, University of Amsterdam AMC, The Netherlands.
authors.forename: W H
authors.initials: WH
authors.lastname: Lamers
authors.forename: J W
authors.initials: JW
authors.lastname: Janzen
authors.forename: A F
authors.initials: AF
authors.lastname: Moorman
authors.forename: R
authors.initials: R
authors.lastname: Charles
authors.forename: E
authors.initials: E
authors.lastname: Knecht
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Ramón
authors.forename: J
authors.initials: J
authors.lastname: Hernández-Yago
authors.forename: S
authors.initials: S
authors.lastname: Grisolía
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D005404:Fixatives; D005938:Glucocorticoids / Q000494:pharmacology; D005969:Glutamate Dehydrogenase / Q000032:analysis* / Q000378:metabolism; D006503:Hepatic Veins; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D011169:Portal Vein; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D005404:Fixatives; D005938:Glucocorticoids; D005969:Glutamate Dehydrogenase; D002222:Carbamoyl-Phosphate Synthase (Ammonia)