A DNA sequence which shows genomic variation in a, alpha and HO strains of Saccharomyces cerevisiae.
HindIII digested DNA from various mutant strains of Saccharomyces cerevisiae probed with a 340 bp nucleotide sequence in M13mp8 derived from a mouse liver cDNA clone p1581 showed strong hybridization to a 4.1 kb DNA fragment class. This was limited to the DNA of cells of alpha mating type but the fragments concerned apparently do not originate from chromosome III. The pattern of hybridization was modified in strains carrying the HO gene consistent with there being extra copies of the Bkm-homologous sequence in these cells. Northern analysis of RNA from cells synchronised in various stages of the mitotic and meiotic cell cycle probed with M13mp8/p1581 indicated related transcripts in meiotic cells.
Gupta
N J
NJ
Genetics Department, University of Edinburgh, UK.
Jones
K W
KW
D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005796:Genes; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D014644:Genetic Variation; D005838:Genotype; D000072235:Mating Factor; D009154:Mutation; D010455:Peptides / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D004271:DNA, Fungal; D010455:Peptides; D000072235:Mating Factor