Chronic ethanol consumption inhibits repair of dimethylnitrosamine-induced DNA alkylation.
Chronic ethanol consumption causes a DNA repair deficiency. This was demonstrated in Sprague-Dawley rats injected with 14C-labeled dimethylnitrosamine after being pair-fed isocaloric, ethanol, or carbohydrate control diets for 4 weeks. Hepatic DNA was isolated from rats killed at intervals over a 36 hour period after administration of the nitrosamine and concentrations of alkylated guanine derivatives were measured. While N7-methylguanine was lost at equivalent rates from the DNA of both diet groups, 06methylguanine, a promutagenic lesion, persisted at higher levels for longer periods of time in the DNA from the alcohol-fed animals.
Mufti
S I
SI
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.
Salvagnini
M
M
Lieber
C S
CS
Garro
A J
AJ
D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D004247:DNA / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D004128:Dimethylnitrosamine / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008745:Methylation; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D002250:Carbon Radioisotopes; D000431:Ethanol; D004247:DNA; D004128:Dimethylnitrosamine