title: Synthesis of enzymatically active D-7,8-dihydroneopterin-3'-triphosphate.
abstract: The first chemical synthesis of D-neopterin-3'-triphosphate and D-7,8-dihydroneopterin-3'-triphosphate is described. D-neopterin-3'-monophosphate was first 1'-2'-0-formylated with anhydrous formic acid, then activated with 1,1'-carbonyldiimidazole and phosphorylated with n-tributyl-ammonium pyrophosphate. The yield of 3'-NTP was 24%. D-7,8-dihydroneopterin-3'-triphosphate was obtained by chemical (hyposulfite) or catalytic (Pd:H2) reduction of 3'-NTP. Preparations from both reductions were fully active in two different enzymatic systems: synthesis of L-5,6,7,8-tetrahydrobiopterin and in the C-2'-epimerization reaction to L-7,8-dihydromonapterin-3'-triphosphate.
authors: Department of Pediatrics, University of Zurich, Switzerland.
authors: B
authors: B
authors: Zagalak
authors: F
authors: F
authors: Neuheiser
authors: U
authors: U
authors: Redweik
authors: R
authors: R
authors: Bosshard
authors: W
authors: W
authors: Leimbacher
mesh_terms: D001708:Biopterins / Q000031:analogs & derivatives / Q000138:chemical synthesis; D008722:Methods; D019798:Neopterin / Q000031:analogs & derivatives; D011621:Pteridines / Q000138:chemical synthesis*
chemical_list: D011621:Pteridines; C013490:dihydroneopterin triphosphate; D001708:Biopterins; C055979:neopterin-3'-triphosphate; D019798:Neopterin; C003402:sapropterin