Melatonin and prolactin concentrations in blood of cattle exposed to 8, 16 or 24 hours of daily light.
In two experiments, 17-wk-old Holstein bulls exposed to 16 (Exp. 1) or 24 h (Exp. 2) of light daily were compared with bulls given 8 h of light. Blood was sampled at 30-min or 120-min intervals for 48 h at the beginning and again after 4 wk of light treatment. Melatonin concentrations varied episodically in serum, and means were 1.6-fold to 5.1-fold greater during darkness than during light periods. Continuous lighting abolished the nocturnal increase in concentrations of melatonin in three of four calves. Prolactin (PRL) was greater (P less than .05) in calves receiving 16 h (30.9 ng/ml of serum) than in calves receiving 8 h (7.0 ng/ml) of light daily. Prolactin was not different between calves receiving 24 or 8 h of light daily. In a third experiment, one pinealectomized (PX) and two sham PX (SPX) calves were exposed to continuous lighting and infused with melatonin for 16 h/d for 5 wk, and one PX and two SPX calves were infused for 8 h daily. Melatonin infusion increased average concentrations of melatonin in serum 7.2-fold to 18-fold above baseline concentrations. Duration of melatonin infusion did not affect PRL (21.0 vs 20.8 ng/ml of serum). Also, surgical treatment did not affect PRL concentrations. Similarly, in a fourth experiment, PRL in postpubertal heifers fed melatonin to mimic and 8L:16D photoperiod averaged 27.1 ng/ml of serum, which was not different from PRL in heifers receiving 16L:8D and fed vehicle (32.6 ng/ml). We conclude that PRL and melatonin are each affected by photoperiod but are not casually related in cattle.
Stanisiewski
E P
EP
Dept. of Anim. Sci., Michigan State University, East Lansing 48824.
Chapin
L T
LT
Ames
N K
NK
Zinn
S A
SA
Tucker
H A
HA
D000818:Animals; D002417:Cattle / Q000097:blood*; D008027:Light; D008297:Male; D008550:Melatonin / Q000097:blood*; D010507:Periodicity; D011388:Prolactin / Q000097:blood*
D011388:Prolactin; D008550:Melatonin