Application of 30-MHz acoustic scattering to the study of human red blood cells.
A technique for simultaneously measuring the scattering amplitude of individual particles at two angles is applied to human red blood cells. Using a Rayleigh scattering model, the density and compressibility of the cells may be determined given a priori knowledge of their volume. A calibration method relying on measurements of the bulk properties of particle suspensions is described. Red cell properties in hypotonic and hypertonic hosts are compared with a homogeneous mixture model, and a linear relation between hemoglobin content and scattering amplitude at a 90 deg scattering angle is established.
Roos
M S
MS
Applied Mechanics, Yale University, New Haven, Connecticut 06520.
Apfel
R E
RE
Wardlaw
S C
SC
D002138:Calibration; D004912:Erythrocytes / Q000378:metabolism / Q000502:physiology*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D008863:Microspheres; D008962:Models, Theoretical; D012542:Scattering, Radiation; D013016:Sound
D006454:Hemoglobins; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions