The antiproliferative action of deoxyspergualin is different from that induced by amine oxidase.
The amine oxidase activities contained in calf serum and human serum were detected at levels of 90.8 and less than 0.1 nmol O2/minute/ml serum, respectively, by measuring oxygen consumption coupled with spermidine oxidation. Deoxyspergualin (NKT-01) and spergualin (SGL) containing spermidine in their structure were also oxidized in calf serum at the rate of 3.6 and 11.6 nmol O2/minute/ml serum, respectively. To investigate whether amine oxidase is essential for NKT-01 and SGL to exhibit their antiproliferative activities or not, the in vitro activities of NKT-01, SGL and polyamines against L1210 cells were examined in the presence of calf or human serum. Polyamines exhibited antiproliferative activity only in the presence of calf serum, while NKT-01 and SGL inhibited cell growth in the presence of both calf and human serum. In the presence of calf serum the activity of NKT-01 was inhibited by aminoguanidine, an amine oxidase inhibitor. Aminoguanidine did not inhibit the activity of NKT-01 in the presence of human serum. The activity of NKT-01 was shown at much lower concentrations in the presence of human serum than that in the presence of calf serum, and was strongly dependent on incubation time. The in vivo activities of NKT-01, SGL and SGL derivatives correlated with their in vitro activities in the presence of human serum. These results suggest that the in vivo antitumor activities of NKT-01, SGL and SGL derivatives may be attributed to a mechanism different from those of amine oxidase-oxidized product and represent a novel growth inhibitory action.
Kuramochi
H
H
Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd., Tokyo, Japan.
Hiratsuka
M
M
Nagamine
S
S
Takahashi
K
K
Nakamura
T
T
Takeuchi
T
T
Umezawa
H
H
D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006146:Guanidines / Q000037:antagonists & inhibitors / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use; D007939:Leukemia L1210 / Q000188:drug therapy*; D051379:Mice; D008811:Mice, Inbred DBA; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000502:physiology*; D010101:Oxygen Consumption; D013045:Species Specificity
D006146:Guanidines; C033797:spergualin; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors; C004479:pimagedine; C037258:gusperimus