title: Tumorigenicity and oncogene expression in pediatric cancers.
abstract: Cytogenetic and epidemiological studies of pediatric cancers have implicated a loss of genetic information in the development of these tumors. In contrast, other studies have shown that activation of endogenous oncogenes is a common event in these cancer cells. The technique of somatic cell hybridization provides a model for investigating the interaction between loss of genetic elements and oncogene activation in pediatric cancers. A variety of human-human cell hybrids were formed between a tumorigenic adult carcinoma and representative tumorigenic pediatric cell lines. All hybrid cells were completely suppressed for tumor-forming ability when assayed in nu/nu (nude) mice. When the expression of the N-myc, c-myc, and sis oncogenes and tumorigenicity were examined in the same hybrid cells, no correlation was found, suggesting that the expression of these oncogenes in these hybrid cells did not appear to be controlled by putative "tumor suppressor" genes. Thus, tumorigenicity behaves as a recessive genetic trait in pediatric cancers. Furthermore, different genetic elements may be lost during tumor development of adult cancers as opposed to pediatric cancers.
authors: Division of Hematology-Oncology, Children's Hospital of Los Angeles, California.
authors: S R
authors: SR
authors: Pasquale
authors: G R
authors: GR
authors: Jones
authors: C J
authors: CJ
authors: Doersen
authors: B E
authors: BE
authors: Weissman
mesh_terms: D002648:Child; D005808:Genes, Recessive; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D009369:Neoplasms / Q000235:genetics*; D009857:Oncogenes; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger