Replication kinetics of X chromosomes in fibroblasts and lymphocytes.
The kinetics of replication for early and late replicating X chromosomes in karyotypically normal fibroblasts and lymphocytes was studied using terminal bromodeoxyuridine (BrdU) treatment followed by Hoechst/light/Giemsa staining. Although the order of band appearance differs between the two tissues, the programme (order and interval between band appearances) for early replicating bands (dark R-bands) is identical in the two homologues. This is probably also the case for later replicating bands (dark G-bands) though the criteria for determining mean band appearance times are less reliable for these bands when terminal BrdU treatment is used. This means that the late X has a delayed start but thereafter proceeds at the same pace as its early counterpart.
Reddy
K S
KS
MRC Radiobiology Unit, Chilton, Didcot, UK.
Savage
J R
JR
Papworth
D G
DG
D001973:Bromodeoxyuridine; D002453:Cell Cycle; D002478:Cells, Cultured; D002871:Chromosome Banding; D004261:DNA Replication; D005347:Fibroblasts / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000648:ultrastructure; D014960:X Chromosome
D001973:Bromodeoxyuridine