Temperature affects binding of murine monoclonal IgG antibodies to protein A.
Murine IgG subclasses show different affinities when they react with protein A. The temperature of application of the sample to protein A-Sepharose markedly influences the interaction of certain antibodies with this specific adsorbent. Elution of weakly bound mouse IgG could be carried out by mild temperature changes instead of lowering the pH, and this should be advantageous with certain immunoglobulins that are labile under acidic conditions.
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Center for Molecular Medicine and Immunology, University of Medicine and Dentistry of New Jersey, Newark 07103.
Primus
F J
FJ
Goldenberg
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000302:isolation & purification; D000912:Antibodies, Neoplasm / Q000032:analysis / Q000302:isolation & purification; D001666:Binding Sites, Antibody; D002272:Carcinoembryonic Antigen / Q000276:immunology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism*; D051379:Mice; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013696:Temperature
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A