Scintillation counting of 14C-labeled soluble and insoluble compounds in plant tissue.
A method is described for the liquid scintillation counting of 14C in plant tissues. Samples are fixed, in the scintillation vial, in a solution of ethanol and acetic acid (3:1) and decolorized with commercial bleach before the addition of scintillation liquid. The method was compared to other techniques of tissue oxidation or digestion and found to be equally effective at least with thin tissue samples. The technique is simple, rapid, and inexpensive and does not result in loss of 14C.
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Division of Biological Sciences, Cornell University, Ithaca, New York 14853.
Wimmers
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Turgeon
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D002250:Carbon Radioisotopes; D007553:Isotope Labeling; D010944:Plants / Q000032:analysis*; D012588:Scintillation Counting; D012995:Solubility
D002250:Carbon Radioisotopes