Periodic cleavage of poly(dA) by oligothymidylates covalently linked to the 1,10-phenanthroline-copper complex.
1,10-Phenanthroline (OP) was covalently attached to the 3'-terminus of two oligothymidylates via different linkers [abbreviated as T8-(OP) and T6-(OP)]. In the presence of Cu2+ and 3-mercaptopropionic acid (MPA), these reagents induce a hybridization-dependent cleavage of poly(dA) and of a 27 nucleotide long oligodeoxynucleotide containing an A8 sequence. The principal cleavage sites on the 27-mer span four residues located near the 3'-terminal phosphate group of T8-(OP). When poly(dA) was degraded by T6-(OP) and T8-(OP), a series of bands were obtained corresponding to a repeat unit of six and eight nucleotides, respectively. This periodicity reflects the cooperative binding of oligothymidylate-OP to the polynucleotide matrix and the localized nicking sites.
François
J C
JC
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U.201, CNRS UA.481, Paris, France.
Saison-Behmoaras
T
T
Chassignol
M
M
Thuong
N T
NT
Sun
J S
JS
Hélène
C
C
D001483:Base Sequence; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010618:Phenanthrolines; D011061:Poly A
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010618:Phenanthrolines; C027903:oligo (dT); D011061:Poly A; C015465:poly(dA); C025205:1,10-phenanthroline