Effect of recombinant human interferon-alpha A/D on in vivo murine tumor cell growth.
We investigated the effect of human recombinant interferon-alpha A/D A/D-IFN), which is known to delay the growth of murine tumor cells, on the growth of S1 and R1 subline cells of murine Meth A fibrosarcoma in the peritoneal cavity of mice. In vitro growth of S1 cells was sensitive to, and that of R1 cells was resistant to, the direct effect of A/D-IFN, as with murine natural IFN-alpha/beta, which was used originally to isolate these sublines. In vivo, however, the growth of not only S1 cells but also R1 cells was suppressed by the administration of A/D-IFN, and the survival time of tumor-bearing mice was prolonged. Although A/D-IFN had a direct effect on S1 cells in vivo, R1 cells were susceptible only to the indirect effect via the host cells. Macrophages (M phi) harvested from the peritoneal cavity of A/D-IFN-treated mice bearing ascitic R1 cells were very effective in suppressing the in vitro growth of R1 cells; those from non-R1-bearing A/D-IFN-treated mice were less effective. The results of in vitro experiments indicate that M phi are very probably activated by the synergism of A/D-IFN and M phi diameter-activating factor(s) produced by lymphoid cells in tumor-bearing mice.
Uno
K
K
Department of Zoology, Faculty of Science, Kyoto University, Japan.
Shimizu
S
S
Inaba
K
K
Kitaura
M
M
Nakahira
K
K
Kato
T
T
Yamaguchi
Y
Y
Muramatsu
S
S
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000473:pathology*; D010529:Peritoneal Cavity / Q000473:pathology
D007370:Interferon Type I