title: Effect of a platelet release fraction on glycosaminoglycan synthesis by cultured dermal fibroblasts from patients with progressive systemic sclerosis.
abstract: Confluent cultures of dermal fibroblasts from patients with progressive systemic sclerosis (PSS) and healthy controls were investigated for the synthesis of glycosaminoglycans (GAG) in response to a platelet release fraction (PRF) obtained by treatment of pooled platelets from normal individuals with adenosine 5'-diphosphate (ADP). PRF, at concentrations of 1 to 50 micrograms protein/ml, produced a linear increase in GAG synthesis that was always greater in cultures of PSS fibroblasts than in cultures of normal fibroblasts (P less than 0.001). A partial inhibition of GAG synthesis was observed with 100 micrograms/ml of PRF. The increased GAG synthesis in cultures incubated with PRF was not due to ADP. These findings demonstrate a difference in response to PRF between PSS and normal fibroblasts and may reflect an increased responsiveness of PSS fibroblasts to platelet factors.
authors: Department of Dermatology and Cutaneous Surgery, University of Miami, School of Medicine, Florida 33101.
authors: V
authors: V
authors: Falanga
authors: S P
authors: SP
authors: Alstadt
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D008875:Middle Aged; D012595:Scleroderma, Systemic / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D000244:Adenosine Diphosphate