Type V collagen selectively inhibits human endothelial cell proliferation.
Type V collagen from human placenta remarkably inhibited human umbilical vein endothelial cell (HUVEC) proliferation in a dose-dependent manner when coated on the culture dishes. Other types of collagen (I, III, IV) and fibronectin enhanced HUVEC proliferation under the same conditions. The inhibitory activity of type V collagen was seen not only when it was coated on the dishes, but also when it was directly added into cell culture. The attachment effect of type V collagen did not differ from that of type I collagen. The inhibitory activity is a phenomenon selective for endothelial cells, since type V collagen did not affect the proliferation of human umbilical vein smooth muscle cells, aortic smooth muscle cells, or nasal mucosa fibroblasts.
Fukuda
K
K
Department of Otolaryngology, Kagoshima University, School of Medicine, Japan.
Koshihara
Y
Y
Oda
H
H
Ohyama
M
M
Ooyama
T
T
D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D014357:Trypsin / Q000378:metabolism
D003094:Collagen; D014357:Trypsin