A simple fluorometric method for the determination of serum free carnitine.
A new fluorometric method for the determination of serum free carnitine is described. The addition of carnitine to a system containing carnitine acetyltransferase (EC 2.3.1.7) and acetyl-CoA gives rise to the formation of CoA. The system is coupled to N-(p-(2-benzimidazolyl)-phenyl)-maleimide (BIPM). A fluorescent agent, CoA-BIPM, is produced proportionally to concentration of carnitine. By measuring the fluorescence intensity of BIPM, the carnitine content of serum can be determined. The coefficients of variation, within-run and between-run, of the method were 5.2 and 2.6%, respectively. Recovery of carnitine added to serum was 98-113%. Comparison with a colorimetric method showed a good correlation (r greater than 0.90). The method has sufficient sensitivity to measure concentrations as low as 10 mumol/l.
Maehara
M
M
Department of Pediatrics, Nagoya University School of Medicine, Aichi, Japan.
Kinoshita
S
S
Watanabe
K
K
D000328:Adult; D002331:Carnitine / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D005470:Fluorometry; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008832:Microchemistry
D002331:Carnitine