Loss of antigenic properties of acetylcholine receptors in rat skeletal muscle after birth.
Antibodies in a myasthenic serum were used to follow the changes in antigenic properties of acetylcholine receptors in rat skeletal muscle during development. In binding assays at saturating concentrations of antigen or antibody, the antibodies reacted with extrajunctional receptors of fetal and denervated adult muscle but showed little binding to junctional receptors of adult rat muscle. They did, however, bind to junctional receptors of adult chicken muscle which, unlike rat receptors, do not appear to undergo a change in their channel properties during development. Binding studies with acetylcholine receptors of developing rat muscle carried out at saturating concentrations of antibody showed that the loss of antigenic determinant(s) begins at 1-2 days after birth.
O'Malley
J P
JP
Department of Physiology, University of Otago Medical School, Dunedin, New Zealand.
Wilson
S J
SJ
Bray
J J
JJ
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D002645:Chickens / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D024510:Muscle Development; D009132:Muscles / Q000196:embryology / Q000378:metabolism*; D009135:Muscular Diseases / Q000276:immunology; D051381:Rats / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D000941:Antigens; D011950:Receptors, Cholinergic