title: Vasopressin is the only component of serum-free medium that stimulates phosphatidylcholine hydrolysis and accumulation of diacylglycerol in cultured REF52 cells.
abstract: Vasopressin stimulates phosphatidylcholine hydrolysis in REF52 cells, and this phosphatidylcholine hydrolysis results in increases in choline containing metabolites in the culture medium (2.3 x control levels) and accumulation of cellular diacylglycerol (6.5 x control levels). Vasopressin is the only component of a 6-component mixture of the serum-free medium for REF52 cells that induces the phosphatidylcholine hydrolysis response. The effect of vasopressin is both time- and concentration-dependent. Maximal levels of both phosphatidyl-choline hydrolysis and accumulation of diacylglycerol are observed between 10 and 20 min after treatment with vasopressin. Effects are maximal at vasopressin concentrations of 100 ng/ml; the ED50 for vasopressin-stimulated phosphatidyl-choline hydrolysis is approximately 0.7 ng/ml. The evolution of diacylglycerol occurs in a time frame that is consistent with the diacylglycerol activating protein kinase C in a "second phase" agonist response.
authors: Lipid Biochemistry Laboratory, W. Alton Jones Cell Science Center, Inc., Lake Placid, NY 12946.
authors: C J
authors: CJ
authors: Welsh
authors: H T
authors: HT
authors: Cao
authors: H
authors: H
authors: Chabbott
authors: M C
authors: MC
authors: Cabot
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D002460:Cell Line; D003470:Culture Media / Q000032:analysis*; D004075:Diglycerides / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D005989:Glycerides / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D014667:Vasopressins / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D004075:Diglycerides; D005989:Glycerides; D010713:Phosphatidylcholines; D014667:Vasopressins