Low [3H]cytochalasin B binding in the cerebral cortex of newborn rat.
The concentrations of glucose transporter in the cerebral cortex and brainstem of neonatal (4-7 days old) and adult rats were measured using [3H]cytochalasin B binding. There was significantly lower binding in neonatal cortex (1.9 +/- 0.7 pmol/mg protein) compared to adult (8.9 +/- 2.5 pmol/mg protein). Scatchard analysis indicates this difference is due to a lower Bmax (neonate, 9.7 pmol/mg protein; adult, 18.6 +/- 1.3 pmol/mg protein). Measurement of [3H]cytochalasin B binding in microvessels prepared from cortex of adult (28.1 +/- 3.5 pmol/mg protein) and neonate (12.8 +/- 1.9 pmol/mg protein) indicates a lower binding in the microvasculature of neonates, whereas no such difference was seen in the binding in microvessels prepared from adult and neonatal brainstem (adult, 11.8 +/- 2.3 pmol/mg protein; neonate, 9.4 +/- 2.7 pmol/mg protein). In both adult and neonate brain, there is an enrichment of glucose transporters in the microvasculature.
Morin
A M
AM
Neurology Research Laboratory, Veterans Administration Medical Center, Sepulveda, CA 91343.
Dwyer
B E
BE
Fujikawa
D G
DG
Wasterlain
C G
CG
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001933:Brain Stem / Q000098:blood supply / Q000378:metabolism; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism*; D003571:Cytochalasin B / Q000378:metabolism*; D005260:Female; D008297:Male; D008833:Microcirculation / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D010693:Phloretin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B; D010693:Phloretin