A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue.
A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.
Knights
K M
KM
Department of Clinical Pharmacology, Flinders University of South Australia.
Drew
R
R
D000818:Animals; D002250:Carbon Radioisotopes; D003065:Coenzyme A / Q000032:analysis*; D003066:Coenzyme A Ligases; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D008832:Microchemistry; D008930:Mitochondria, Liver / Q000201:enzymology; D017830:Octoxynol; D019308:Palmitic Acid; D010169:Palmitic Acids; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D010169:Palmitic Acids; D019308:Palmitic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; D003066:Coenzyme A Ligases; D003065:Coenzyme A