Sulfated glycosaminoglycans in rat bronchopulmonary lavage: detection after in vivo labelling.
Sulfated macromolecules occurring in bronchoalveolar lavage fluid supernatant were radiolabelled in vivo by 35SO4, that was insufflated via trachea. DEAE-Sephacel chromatography dissociated sulfated glycoproteins, presumably of tracheobronchial mucus origin, from a minor, but heavily labelled component. Degradative analysis and acetyl cellulose electrophoresis identified this component as a mixture of heparan sulfate and chondroitin sulfate proteoglycans.
Vilim
V
V
Institute of Hygiene and Epidemiology, Centre of Industrial Hygiene and Occupational Diseases, Praha, Czechoslovakia.
Hurych
J
J
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000302:isolation & purification*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D007507:Therapeutic Irrigation
D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes