Cloning, nucleotide sequence, and taxonomic implications of the flagellin gene of Roseburia cecicola.
The gene coding for the flagellin protein of Roseburia cecicola, an oxygen-intolerant, gram-negative, anaerobic bacterium indigenous to the murine cecum, has been cloned and sequenced. NH2-terminal amino acid sequence data from the flagellin protein were used as a basis for the synthesis of two mixed-sequence deoxyoligonucleotides. The oligonucleotides were used to identify and clone the flagellin structural gene. DNA sequence analysis of M13mp8 and mp9 subclones revealed a protein with a length of 293 amino acids and a molecular weight of 31,370. Comparisons with the sequences of flagellins of other species revealed conserved regions and suggested that although R. cecicola has structural characteristics of a gram-negative bacterium, it may be most closely related to the gram-positive bacteria.
Martin
J H
JH
Department of Microbiology, University of Illinois, Urbana 61801.
Savage
D C
DC
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005408:Flagellin / Q000235:genetics*; D005796:Genes; D006090:Gram-Negative Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data
D001426:Bacterial Proteins; D005408:Flagellin