Solid-phase radioimmunoassay of human complement fragment C5a.
In this competitive RIA for determining concentrations of human C5a in biological fluids and in buffers, labeled C5a and sample are allowed to compete for binding to a limited amount of goat antibody to human C5a in solution. Free and bound tracer are then separated by a second antibody (rabbit anti-goat IgG) immobilized on paramagnetic particles. Total incubation time for this assay is 70 min. Sensitivity, precision, and analytical recovery of this assay compare well with those of a reference method.
Chang
S
S
Ciba-Corning Diagnostics, E. Walpole, MA 02032.
Leo-Mensah
A
A
Campbell
J
J
Stastny
M
M
Patrick
R A
RA
D001667:Binding, Competitive; D003182:Complement C5 / Q000032:analysis*; D015936:Complement C5a; D004355:Drug Stability; D006358:Hot Temperature; D006801:Humans; D011863:Radioimmunoassay / Q000592:standards; D011933:Reagent Kits, Diagnostic
D003182:Complement C5; D011933:Reagent Kits, Diagnostic; D015936:Complement C5a