Anion activation of dopamine beta-hydroxylase: a kinetic model.
1. A theoretical analysis has been made of the mechanism of anion activation of dopamine beta-hydroxylase on the basis of accumulated experimental data. A model is presented that accounts for the numerous different effects of activating anions on the enzyme kinetics. This model has a general validity, since it holds for any of the kinetic mechanisms thus far proposed for dopamine beta-hydroxylase. 2. It has been shown that the results of this analysis have direct implications for the experimental conditions to be used in the study of the dopamine beta-hydroxylase reaction. 3. The present analysis has proved that, under appropriate assumptions, theoretical treatment of nonessential activation, so far limited to the single-substrate case, can be easily extended to steady-state multireactant enzymes.
Palatini
P
P
Department of Pharmacology, University of Padova, Italy.
D000465:Algorithms; D000838:Anions / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D004789:Enzyme Activation; D007700:Kinetics; D008954:Models, Biological
D000838:Anions; D004299:Dopamine beta-Hydroxylase; D001205:Ascorbic Acid