Asymptomatic association of hemoglobin Dunn (alpha 6[A4]Asp----Asn) and hemoglobin O-Arab (beta 121[GH4]Glu----Lys) in a Moroccan man.
We report on the association of Hb Dunn (alpha 6[A4]Asp----Asn) and Hb O-Arab (beta 121 [GH4]Glu----Lys) in a healthy Moroccan man. Hb Dunn had the same electrophoretic properties as Hb G-Philadelphia, but its percentage was lower. Its identification was based on sequence determination of the alpha T1 peptide. Bgl II and Eco RI mapping showed the presence of four alpha-genes. Hb O-Arab was easily recognized through its electrophoretic properties and was confirmed by the suppression of the Eco RI site located in exon 3 of the beta-gene. The percentages of the various hemoglobins showed that the doubly mutated hemoglobin Dunn/O-Arab has a normal stability and suggested that the Dunn mutation is carried by the alpha 1-gene. In cord blood [propositus's son], the output of the alpha Dunn gene was found equivalent to that existing in the adult.
Baklouti
F
F
CNRS UA 1171, Faculté de Médecine Grange-Blanche, Lyon, France.
Francina
A
A
Dorléac
E
E
Baudin-Chich
V
V
Gombaud-Saintonge
G
G
Plauchu
H
H
Wajcman
H
H
Delaunay
J
J
Godet
J
J
D002851:Chromatography, High Pressure Liquid; D006455:Hemoglobins, Abnormal / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D009018:Morocco; D010446:Peptide Fragments / Q000302:isolation & purification; D014357:Trypsin
D006455:Hemoglobins, Abnormal; D010446:Peptide Fragments; C040493:hemoglobin Dunn; C032031:hemoglobin Arab; D014357:Trypsin