A rapid and sensitive method for the determination of paraquat in plasma and urine by thin-layer chromatography with flame ionization detection.
A new method for the determination of paraquat in biological fluids has been developed. It involves thin-layer chromatography with flame ionization detection (TLC/FID) and simple solid-phase extraction with a disposable octadecylsilane column. This procedure allows direct loading of the samples on the disposable column. When Chromarod SII is used as the stationary phase and methanol-2N hydrochloric acid (2:3, v/v) as the mobile phase, recovery levels of paraquat in spiked materials are from 87.5 to 98.0%. This method can be used for samples of paraquat at levels as low as 50 ng.
Ikebuchi
J
J
Department of Legal Medicine, Tottori University School of Medicine, Yonago, Japan.
Yuasa
I
I
Kotoku
S
S
D000328:Adult; D002855:Chromatography, Thin Layer; D005260:Female; D005410:Flame Ionization; D006801:Humans; D008297:Male; D008832:Microchemistry; D008875:Middle Aged; D010269:Paraquat / Q000032:analysis*
D010269:Paraquat