Affinity chromatography of a sequence-specific DNA binding protein using Teflon-linked oligonucleotides.
An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated on an automated DNA synthesizer, contains high levels of attached DNA, and has superior mechanical properties. It should be generally useful for affinity chromatography of DNA binding proteins.
Duncan
C H
CH
Division of Basic Science, Children's Hospital Research Foundation, Cincinnati, Ohio 45229.
Cavalier
S L
SL
D001483:Base Sequence; D002846:Chromatography, Affinity; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000138:chemical synthesis; D011138:Polytetrafluoroethylene
D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D011138:Polytetrafluoroethylene