Liquid-chromatographic determination of free and total 3,4-dihydroxyphenylglycol and 3-methoxy-4-hydroxyphenylglycol in urine.
We describe the conjoint measurement in urine of the alcoholic catecholamine metabolites 3,4-dihydroxyphenylglycol (DHPG) and 3-methoxy-4-hydroxyphenylglycol (MHPG). The method involves extraction with ethyl acetate and back-extraction into acetic acid (MHPG), followed by adsorption onto alumina (DHPG). The separation is performed by reversed-phase liquid chromatography with column switching and amperometric detection. Total (free plus conjugated) compounds are determined after a 24-h enzymatic hydrolysis. Twelve-hour excretion values for normal and hypertensive subjects are given, together with results obtained for patients with pheochromocytoma, chemodectoma, and congenital dopamine-beta-hydroxylase deficiency. The assay is sensitive, reproducible, and free of interferences, and may lead to a better understanding of overall catecholamine metabolism in health and disease.
Julien
C
C
Department of Physiology and Clinical Pharmacology, (UA CNRS 606), Faculty of Pharmacy, Lyon, France.
Rodriguez
C
C
Sacquet
J
J
Cuisinaud
G
G
Sassard
J
J
D000310:Adrenal Gland Neoplasms / Q000652:urine; D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods; D002940:Circadian Rhythm; D004299:Dopamine beta-Hydroxylase / Q000172:deficiency; D005260:Female; D006018:Glycols / Q000652:urine*; D006801:Humans; D006973:Hypertension / Q000652:urine; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000652:urine*; D010673:Pheochromocytoma / Q000652:urine; D012016:Reference Values
D006018:Glycols; D008734:Methoxyhydroxyphenylglycol; D004299:Dopamine beta-Hydroxylase; C010117:3,4-dihydroxyphenylglycol