A combined recording and microiontophoresis technique for input-output analysis of single neurons in the mammalian CNS.
A method is described for producing electrolytically sharpened and lacquer-insulated steel microelectrodes and for their attachment to a multibarrel micropipet assembly by light-curing bonding compound. This electrode complex is specially designed for recordings from deep brain structures. It permits the analysis of the input signals to and the output signals from a single neuron recorded simultaneously from an extracellular electrode position, termed juxtacellular recording. Furthermore it provides the ability to manipulate pharmacologically the input-output ratio expressed by the neuron under varying sequences of natural stimulation.
Gottschaldt
K M
KM
Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, F.R.G.
Hicks
T P
TP
Vahle-Hinz
C
C
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D005071:Evoked Potentials / Q000187:drug effects; D007478:Iontophoresis / Q000295:instrumentation* / Q000379:methods; D008839:Microelectrodes