title: Trichinella pseudospiralis larvae express natural killer (NK) cell-associated asialo-GM1 antigen and stimulate pulmonary NK activity.
abstract: Natural killer (NK) cell function was evaluated in mice infected with either Trichinella pseudospiralis or T. spiralis larvae. T. pseudospiralis-infected mice consistently demonstrated augmented pulmonary NK cell-mediated clearance of YAC-1 tumor cells in vivo but failed to display enhanced splenic NK cell-mediated lysis of the same tumor cells in vitro. Attempts to alter NK cell function in vivo by the injection of anti-asialo-GM1 antibody resulted in anaphylaxis and death of the hosts infected with T. pseudospiralis but not of mice infected with T. spiralis. Immunofluorescence studies demonstrated that larvae of both species bound large quantities of anti-asialo-GM1 antibody but did not react with another NK-associated antibody, anti-NK-1.1. Even though anti-asialo-GM1 antibody reacted with larvae of both species, intravenous injection of antibody elicited anaphylaxis only in hosts that were infected with T. pseudospiralis.
authors: Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
authors: J Y
authors: JY
authors: Niederkorn
authors: G L
authors: GL
authors: Stewart
authors: S
authors: S
authors: Ghazizadeh
authors: E
authors: E
authors: Mayhew
authors: J
authors: J
authors: Ross
authors: B
authors: B
authors: Fischer
mesh_terms: D000305:Adrenal Cortex Hormones / Q000097:blood; D000707:Anaphylaxis / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000276:immunology*; D007111:Immunity, Cellular; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology*; D008168:Lung / Q000276:immunology; D051379:Mice; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000276:immunology*
chemical_list: D000305:Adrenal Cortex Hormones; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000954:Antigens, Surface; D006028:Glycosphingolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside