Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato.
The nucleotide sequence of a 4.5-kilobase copper resistance determinant from Pseudomonas syringae pv. tomato revealed four open reading frames (ORFs) in the same orientation. Deletion and site-specific mutational analyses indicated that the first two ORFs were essential for copper resistance; the last two ORFs were required for full resistance, but low-level resistance could be conferred in their absence. Five highly conserved, direct 24-base repeats were found near the beginning of the second ORF, and a similar, but less conserved, repeated region was found in the middle of the first ORF.
Mellano
M A
MA
Department of Plant Pathology, University of California, Riverside 92521.
Cooksey
D A
DA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D003300:Copper / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics*
D003300:Copper