title: Thyrotropic activity of salmon pituitary glycoprotein hormones in the Hawaiian parrotfish thyroid in vitro.
abstract: The thyrotropic activities of salmon pituitary extract, thyroid-stimulating hormone (TSH), gonadotropins (GTH), and glycoprotein fractions obtained during purification of salmon TSH and GTH were measured using the parrotfish thyroid culture system. Purified salmon TSH was approximately 1,000 times more potent than bovine TSH in stimulating thyroxine release into the culture medium. Most of the forms of salmon GTH had no thyrotropic activity. One of the forms of salmon GTH (GTH-F) and three chromatofocusing fractions (CF-B, -C, and -E) that were devoid of activity in the coho salmon in vivo had some thyrotropic activity in the parrotfish thyroid culture. Whether the activity of these fractions was due to contamination with TSH, less potent forms of TSH, or inherent thyrotropic activity of a form of GTH is discussed. These results indicate that the parrotfish thyroid culture system can be used to detect thyrotropic activity of fractions obtained during the purification of teleost TSH.
authors: Department of Zoology, University of Washington, Seattle 98195.
authors: P
authors: P
authors: Swanson
authors: E G
authors: EG
authors: Grau
authors: L M
authors: LM
authors: Helms
authors: W W
authors: WW
authors: Dickhoff
mesh_terms: D000818:Animals; D002417:Cattle; D005399:Fishes; D006065:Gonadotropins, Pituitary / Q000494:pharmacology; D009924:Organ Culture Techniques; D010907:Pituitary Hormones / Q000494:pharmacology*; D012474:Salmon; D013045:Species Specificity; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology*; D013974:Thyroxine / Q000378:metabolism
chemical_list: D006065:Gonadotropins, Pituitary; D010907:Pituitary Hormones; D013972:Thyrotropin; D013974:Thyroxine