The effect of inosine on the post ischemic heart as bio-energy recovering factor in 31P-MRS.
Perfused guinea-pig hearts, which were analyzed by 31P-MRS, were subjected to 30 and 60 minute ischemia and reperfused using two perfusates, one containing 200 microM inosine, and the other without inosine. After 4 hour reperfusion with inosine, ATP levels increased to 95.5% of preischemic value (30 minute ischemia) and 76.2% (60 minute ischemia). However, after 4 hour reperfusion without inosine, ATP levels increased only to 72.2% (30 minute ischemia) and to 48.2% (60 minute ischemia). In 60 minute ischemic hearts reperfused with inosine, left ventricular maximal positive dp/dt (LV dp/dt) was improved significantly to 82.4% after 6 hour reperfusion in contrast to hearts reperfused without inosine (43.1%). Administration of inosine was very useful for increasing myocardial gross energy product and improving cardiac performance.
Yoshiyama
M
M
First Department of Internal Medicine, Osaka City University Medical School, Japan.
Sakai
H
H
Teragaki
M
M
Takeuchi
K
K
Takeda
T
T
Ikata
M
M
Ishikawa
M
M
Miura
I
I
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003327:Coronary Disease / Q000503:physiopathology*; D004734:Energy Metabolism / Q000187:drug effects*; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007288:Inosine / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D013997:Time Factors
D010725:Phosphocreatine; D007288:Inosine; D000255:Adenosine Triphosphate