Automated densitometry of polyacrylamide gel electrophoregrams.
A device is described which enables to convert a spectrophotometer in a high performance densitometer. Test samples for the evaluation of densitometric characteristics are presented. The estimations can be carried out in the UV-visible range without applying the common staining procedure, on either cylindrical or plane gel plates in absorption or fluorescence. Polyacrylamide gel migrated commercial protein mixtures as well as A(H1N1) influenza virus and Sendai virus polypeptides were used as biological samples.
Zaharia
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CN
Stefan S. Nicolau Institute of Virology, Bucharest, Romania.
D003720:Densitometry / Q000295:instrumentation* / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods; D014773:Virology / Q000295:instrumentation / Q000379:methods