Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase.
There may be a causal relationship between expression of the G2-specific gene nimA and mitotic regulation in Aspergillus. To test this relationship we have introduced extra inducible copies of nimA into Aspergillus and determined the effect of nimA overproduction on mitotic regulation. The results show that nimA overexpression causes mitotic induction in less than a cell cycle and maintains chromatin in a condensed state. These effects occur even if cells are first blocked in S phase. Sequence analysis shows that the nimA gene encodes a potential protein kinase. These data indicate that there is indeed a causal relationship between expression of nimA and the regulation of mitosis and further implicate protein phosphorylation in mitotic control.
Osmani
S A
SA
Department of Pharmacology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
Pu
R T
RT
Morris
N R
NR
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D005796:Genes; D005804:Genes, Lethal; D005809:Genes, Regulator; D005822:Genetic Vectors; D007399:Interphase; D008938:Mitosis; D008969:Molecular Sequence Data; D010957:Plasmids; D011494:Protein Kinases / Q000235:genetics*
D011494:Protein Kinases