Evaluation of an enzyme immunoassay test for the diagnosis of Chlamydia psittaci infection in free-ranging koalas (Phascolarctos cinereus) in southeastern Queensland, Australia.
The IDEIA Chlamydia Test, a commercially available antigen-capture enzyme-linked immunosorbent assay (ELISA) test, based on a monoclonal antibody for the detection of chlamydia in clinical specimens, was evaluated in a population of 65 free-ranging koalas in southeastern Queensland determined to be infected with Chlamydia psittaci. Compared to isolation of the organism in tissue culture, the sensitivity of the IDEIA test ranged from 3 to 11%, and the specificity from 90 to 97%. The results indicated that the IDEIA test is unsuitable for use as a diagnostic screening test for C. psittaci in free-ranging koalas.
Weigler
B J
BJ
Department of Animal Sciences and Production, University of Queensland, St. Lucia, Australia.
Baldock
F C
FC
Girjes
A A
AA
Carrick
F N
FN
Lavin
M F
MF
D000818:Animals; D000835:Animals, Wild; D002690:Chlamydia Infections / Q000175:diagnosis / Q000662:veterinary*; D002691:Chlamydophila psittaci; D003229:Conjunctival Diseases / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D008394:Marsupialia / Q000382:microbiology*; D008403:Mass Screening; D011793:Queensland; D014570:Urologic Diseases / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary