title: Distinct patterns of granulocyte luminol-dependent chemiluminescence response to lectins WGA and RCA-I.
abstract: THe chemiluminescence response was measured in human polymorphonuclear leukocytes (PMNLs) after stimulation with different concentrations of the lectins Triticum vulgaris agglutinin (wheat germ agglutinin, WGA) and Ricinus communis agglutinin I (RCA-I). The two lectins achieved distinct patterns of chemiluminescence; RCA-I evoked a dose-dependent response (0-15 micrograms/ml) with respect to the initial rate of light emission, but the peak value was reached after different lengths of time. A maximum response was obtained after about 25 min with a concentration of 2-4 micrograms/ml. By contrast, WGA caused a bimodal reaction after stimulus addition. In this case the first peak occurred after 15-20 min, and the second peak after around 60 min with maximum effect for 0.5 and 0.25-0.50 micrograms/ml WGA, respectively. The PMNL response is discussed in relation to the regulation of the production of oxygen metabolites and in relation to the pathophysiologic consequences of lectin-mediated activation of phagocytic cells in intestinal epithelium.
authors: Department of Medical Microbiology, Faculty of Health Sciences, University of Linköping, Sweden.
authors: K E
authors: KE
authors: Magnusson
authors: C
authors: C
authors: Dahlgren
authors: A
authors: A
authors: Sjölander
mesh_terms: D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology*; D010084:Oxidation-Reduction; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins / Q000494:pharmacology*
chemical_list: D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014909:Wheat Germ Agglutinins; D008165:Luminol