Seroprevalence and association with abortion of leptospirosis in cattle in Ontario.
Sera were collected using a systematic random sampling from 348 cattle herds in Ontario, in proportion to the cattle population in different areas. One cow in five from 296 dairy herds and one in three from 52 beef herds were sampled. The sera were analyzed for prevalence of antibodies to Leptospira interrogans serovar grippotyphosa, hardjo, icterohaemorhagiae and pomona using the microscopic agglutination test. Herd seroprevalence (one or more animals with titer greater than or equal to 80) in beef and dairy herds combined was grippotyphosa 2%, hardjo 13.8%, icterohaemorrhagiae 10.1% and pomona 25.8%; 39% of all herds showed evidence of leptospiral infection with one or more serovars; 44.2% of 52 beef herds had serological evidence of infection with serovar hardjo compared to 8.4% of 296 dairy herds (P less than 0.0001). Seroprevalence of other serovars was not significantly different between beef and dairy herds. The proportion of beef animals seropositive for hardjo and for pomona increased with age, particularly for hardjo; 26.5% of beef animals aged nine years or over were seropositive for hardjo. Dairy animals showed a significant rise of hardjo but not pomona titers with age. The seroprevalence of pomona infection was significantly higher in dairy cattle in eastern Ontario than in other regions. Thirty-four (6.1%) of 553 aborted bovine fetuses had leptospires detected by immunofluorescence techniques. Sixty-five percent of these fetuses were from submissions made between November and January. Leptospires were identified as serovar hardjo by specific immunofluorescence. There appeared, however, to be a paradoxical serological response in that eight aborting cows had antibody titers to pomona rather than hardjo.(ABSTRACT TRUNCATED AT 250 WORDS)
Prescott
J F
JF
Department of Veterinary Micribiology and Immunology, Ontario Veterinary College, University of Guelph.
Miller
R B
RB
Nicholson
V M
VM
Martin
S W
SW
Lesnick
T
T
D000034:Abortion, Veterinary / Q000453:epidemiology* / Q000276:immunology / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000276:immunology / Q000382:microbiology; D005260:Female; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology*; D007922:Leptospirosis / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D009864:Ontario; D011247:Pregnancy; D012703:Serotyping / Q000662:veterinary
D000907:Antibodies, Bacterial