Isolation of Clostridium spiroforme from rabbits.
The isolation of Clostridium spiroforme from intestinal contents of rabbits was achieved by sampling the supernatant-pellet interphase of centrifuged specimens processed for routine toxin analysis. High-speed centrifugation at 20,000x for 15 minutes provided a rapid and effective means of separating this anaerobic pathogen from the majority of both indigenous and non-indigenous intestinal microbial flora. The unusual helically-coiled, semicircular shape of the microorganism is considered, at least in part, responsible for this phenomenon.
Holmes
H T
HT
Department of Microbiology, Oregon State University, Corvallis 97331.
Sonn
R J
RJ
Patton
N M
NM
D000818:Animals; D002498:Centrifugation; D003013:Clostridium / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D011817:Rabbits / Q000382:microbiology*