A rapid microassay for dirofilarial antibodies by using antigen-coated nitrocellulose paper.
This study describes an enzyme (horseradish peroxidase)-linked immunosorbent assay using nitrocellulose (NC) paper (Dot-ELISA) for the detection of antibodies against Dirofilaria immitis in dogs. Dirofilarial antigens prepared were directly bound on NC paper set into a microfiltration apparatus to ensure their uniformity. Of the 23 infected dogs tested, 21 were determined positive by visual observation of brown-colored spots on NC paper. The positive and negative readings were further confirmed by using a densitometer. Sera from 15 non-infected and 17 other parasite-infected dogs were all negative, i.e. no false positive readings and low cross-reactivities were found using this technique. Also, a large number of samples could be assayed simultaneously within 1.5 h. The Dot-ELISA technique reported here may be useful to veterinarians and also may be a means of field surveying human filariasis.
Matsumura
K
K
Yamaguchi Prefectural Research Institute of Health, Japan.
Kazuta
Y
Y
Endo
R
R
Tanaka
K
K
Inoue
T
T
Matsuda
H
H
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology; D003429:Cross Reactions; D003720:Densitometry; D004183:Dirofilaria immitis / Q000276:immunology*; D004184:Dirofilariasis / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005370:Filarioidea / Q000276:immunology*; D011237:Predictive Value of Tests
D000909:Antibodies, Helminth; D000947:Antigens, Helminth