title: Identification of [hydroxyproline3]-lysyl-bradykinin released from human kininogens by human urinary kallikrein.
abstract: The types of kinins released from purified native, single chain human high and low molecular mass kininogens (HMMKs and LMMKs, respectively) by purified human urinary kallikrein were separated by reverse-phase HPLC and quantitated by the rat uterus bioassay. [Hyp3]-lysyl-bradykinin, a recently discovered kinin, represented up to 58% of the biological activity released from 4 individual HMMK preparations purified from 4 different healthy volunteers. In contrast, the majority of the biological activity released from LMMKs purified from pooled plasma was identified as Lys-bradykinin and [Hyp3]-lysyl-bradykinin represented only 6.4 +/- 3.8%. These findings indicate posttranslation hydroxylation of human kininogens and suggest a preference of HMMKs for this modification.
authors: Department of Medical Physiology, University of Vienna, Austria.
authors: M
authors: M
authors: Maier
authors: G
authors: G
authors: Reissert
authors: I
authors: I
authors: Jerabek
authors: F
authors: F
authors: Lottspeich
authors: B R
authors: BR
authors: Binder
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007609:Kallidin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D007610:Kallikreins / Q000652:urine*; D007704:Kininogens / Q000097:blood*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects
chemical_list: D007704:Kininogens; C054550:bradykinin, (hydroxy-Pro)(3)-Lys-; D007609:Kallidin; D007610:Kallikreins