title: Effect of hypertension on lysosomal enzyme activities in aortic endothelial cells.
abstract: In order to obtain information about the changes in lysosomal enzyme activities in arterial endothelial cells under hypertensive conditions, a biochemical study was performed on 5 lysosomal enzymes, acid phosphatase, N-acetyl-beta-glucosaminidase (NAGase), cathepsin B, cathepsin D and beta-glucuronidase, in endothelial cells isolated by an enzymatic technique from the aorta of spontaneously and renal hypertensive rats, and normotensive control rats. The aortic endothelial cells in the old spontaneously and the renal hypertensive rats showed increased activities of enzymes examined in comparison with those in the age-matched control rats. Endothelial cells in young spontaneously hypertensive rats did not show any elevated enzyme activities compared with those in the controls, and the enzyme activities tended to increase with aging. From this, it is deduced that hypertension activates lysosomal enzyme activities in aortic endothelial cells. The differences in the activities of NAGase, cathepsin B and cathepsin D between hypertensive and control animals increased markedly with advancing age. These activated lysosomal enzymes seem to be involved in the developmental mechanism of arterial endothelial cell injury in hypertension and in further development of hypertensive vascular changes.
authors: Department of Pathology, Shiga University of Medical Science, Japan.
authors: M
authors: M
authors: Sasahara
authors: F
authors: F
authors: Hazama
authors: S
authors: S
authors: Amano
authors: Y
authors: Y
authors: Hayase
authors: N
authors: N
authors: Yukioka
authors: J
authors: J
authors: Kawai
authors: H
authors: H
authors: Kataoka
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001011:Aorta; D002401:Cathepsin B / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D002469:Cell Separation; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D005966:Glucuronidase / Q000378:metabolism; D006973:Hypertension / Q000201:enzymology* / Q000503:physiopathology; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D000135:Acid Phosphatase; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D002401:Cathepsin B; D002402:Cathepsin D