Measurement of ferritin in serum by an indirect competitive enzyme-linked immunosorbant assay.
This indirect competitive enzyme-linked immunosorbant assay (ELISA) for ferritin, unlike other currently available ELISAS, does not require use of an anti-ferritin antibody-enzyme conjugate. Designed for use on microtiter plates, the method has a precision and sensitivity similar to those of other immunoassays. The detection limit is 20 pg of ferritin per test (corresponding to 2.0 micrograms/L in serum samples). Comparison of results obtained on serum from 57 patients by this method with those from a conventional radioimmunoassay gave a correlation coefficient of 0.92.
Polson
R J
RJ
Liver Unit, King's College Hospital, London, U.K.
Kenna
J G
JG
Shears
I P
IP
Bomford
A
A
Williams
R
R
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005293:Ferritins / Q000097:blood*; D006801:Humans; D011863:Radioimmunoassay
D005293:Ferritins