title: Transformation of single myeloid precursor cells by the malignant histiocytosis sarcoma virus (MHSV): generation of growth-factor-independent myeloid colonies and permanent cell lines.
abstract: Direct single-cell assays for oncogenic transformation are available for fibroblasts but not for other cell types. Using malignant histiocytosis sarcoma virus (MHSV), a member of the ras family of retroviruses, in vivo-infected granulocyte/macrophage and macrophage precursor cells lost the requirement for externally added hematopoietic growth factors. Factor-independent growth was demonstrated by colony-transfer experiments. More than 25% of the independent colonies were established as permanent macrophage cell lines following a phase of adaptation to tissue culture conditions. Factor-independent colony growth was also obtained by in vitro infection of single cells. As many as 50% of all myeloid precursor cells were target cells for MHSV as measured by this assay. About 2 x 10(-3) of these colony-forming cells acquired growth factor independence and immortality after in vitro infection. Cell lines derived from these colonies did not require adaptation to tissue culture conditions.
authors: Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
authors: K
authors: K
authors: Klingler
authors: G R
authors: GR
authors: Johnson
authors: N A
authors: NA
authors: Nicola
authors: G
authors: G
authors: Arman
authors: N
authors: N
authors: Kluge
authors: W
authors: W
authors: Ostertag
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D005472:Fluorouracil / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D006378:Helper Viruses / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D012190:Retroviridae / Q000235:genetics*; D013154:Spleen / Q000166:cytology
chemical_list: D006133:Growth Substances; D005472:Fluorouracil