Dynamics of functional maturation and inactivation of HN glycoprotein in NDV-infected chick embryo fibroblasts.
In avirulent NDV strain-infected chick embryo cells treated with cycloheximide at different intervals post infection a decrease of the level of hemagglutinating (HA) and neuraminidase (Nase) activities was observed. Studies on this system led to conclusion that the HA-Nase (HN) glycoprotein molecules are unstable and the actual amount of the functionally active (mature) HN entities is determined by a dynamic equilibrium between the antidromic processes of the HN functional maturation and inactivation. Kinetic studies on the actual intracellular levels of the HA and Nase activities using 5 min intervals of their detection after the cycloheximide treatment permitted to uncouple the processes of the HN maturation and inactivation. Analytical part of the studies made it possible to compute quantitative parameters of the involved processes: (a) pool size of the functionally nonactive HN precursors, (b) time needed for their functional maturation, and (c) rate of their inactivation.
Lipkind
M
M
Department of Biochemistry, Kimron Veterinary Institute, Beit Dagan, Israel.
Urbakh
V
V
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts; D015707:HN Protein; D006389:Hemagglutinins, Viral / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D009439:Neuraminidase / Q000378:metabolism*; D009522:Newcastle disease virus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D012044:Regression Analysis; D014759:Viral Envelope Proteins / Q000378:metabolism*
D015707:HN Protein; D006389:Hemagglutinins, Viral; D014759:Viral Envelope Proteins; D003513:Cycloheximide; D009439:Neuraminidase