Asymmetric transport of D-glucose anomers across the human erythrocyte membrane.
The anomeric preference in the influx and efflux of D-glucose across the human erythrocyte membrane was studied. beta-D-Glucose was transported 1.5 times faster than alpha-D-glucose into the cells, when washed cells were incubated at 20 degrees C in medium containing either alpha- or beta-D-glucose (100 mM). On the other hand, no difference between half-times of efflux of the two anomers was distinguishable. The result demonstrates the presence of influx-efflux asymmetry in anomeric preference in D-glucose transport across the human erythrocyte membrane, and is consistent with the view (Barnett et al., Biochem. J. 145, 417-429, 1975) that the C-1 hydroxyl group of D-glucose interacts with the D-glucose transport protein only in the influx, but not in the efflux.
Miwa
I
I
Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Fujii
H
H
Okuda
J
J
D004910:Erythrocyte Membrane / Q000378:metabolism*; D005947:Glucose / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D007536:Isomerism
D005947:Glucose