Influence of 2-chloroadenosine on the nucleotide content of isolated rat hepatocytes.
2-Chloroadenosine is presumably a non-metabolizable analogue of adenosine; however, this compound induced an increase in the enzymatically measured nucleotide content of isolated rat hepatocytes. HPLC separation and spectral analysis of the peaks showed that this increase may be related to the formation of 2-chloro nucleotides and that the 2-chloro nucleotides appeared in the first minutes of the incubation period. These results demonstrate that 2-chloroadenosine may be metabolized by phosphorylation in rat liver cells.
Claeyssens
S
S
Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, UER Médecine et Pharmacie de Rouen, Saint Etienne du Rouvray, France.
Hamet
M
M
Chedeville
A
A
Basuyau
J P
JP
Lavoinne
A
A
D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000248:Adenosine Kinase / Q000037:antagonists & inhibitors; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009711:Nucleotides / Q000378:metabolism*; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry; D014372:Tubercidin / Q000031:analogs & derivatives / Q000494:pharmacology
D009711:Nucleotides; D010879:Piperazines; D015762:2-Chloroadenosine; C022297:2-chloro-ADP; C034214:5-iodotubercidin; C006979:2-chloro-ATP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C041518:mioflazine; D000248:Adenosine Kinase; D000241:Adenosine; D014372:Tubercidin