Simultaneous electroelution and concentration of DNA fragments from agarose gels.
A rapid and inexpensive method for the electroelution of DNA fragments from agarose gels is described. DNA fragments were separated by agarose gel electrophoresis and visualized by staining with ethidium bromide. Selected DNA fragments were placed into electroeluter tubes capped with dialysis membrane and electroeluted into a small volume of buffer using a conventional horizontal gel electrophoresis apparatus. The method successfully eluted and concentrated DNA fragments with molecular weights ranging from 2.7 to 13.9 MDa in 3 h.
Stroop
W G
WG
Neurovirology Research Laboratory, Veterans Administration Medical Center, Salt Lake City, Utah 84148.
D004247:DNA / Q000302:isolation & purification*; D004563:Electrochemistry / Q000295:instrumentation; D004587:Electrophoresis, Agar Gel / Q000379:methods
D004247:DNA