Autoradiographic studies on glucose utilization in individual zones of the rat thymus.
Wistar rats were injected intraperitoneally with 2-(14C)deoxyglucose and their thymuses were processed for thaw-mount autoradiography after 5, 10 or 35 min. Highest levels of radioactivity were demonstrated in the thymic medulla (5-fold higher than in the cortex). Scanning of autoradiograms for regional differences in grain densities indicated particularly intense glucose utilization in the cortico-medullary zone. Differences in glucose utilization between individual thymic zones seem to reflect differences in cellular composition, i.e., ratio of stroma cells to thymocytes.
Warchol
J B
JB
Department of Histology and Embryology, Medical Academy, Poznań, Poland.
Pilgrim
C
C
Ginda
W J
WJ
Brelińska
R
R
D000818:Animals; D001345:Autoradiography; D003847:Deoxyglucose / Q000493:pharmacokinetics; D005947:Glucose / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000033:anatomy & histology / Q000378:metabolism*
D003847:Deoxyglucose; D005947:Glucose