Separation by high-performance liquid chromatography of alpha- and beta-amino acids: application to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase.
Several pairs of alpha- and beta-amino acids were well separated as the ortho-phthalaldehyde derivatives by reversed phase HPLC. These included alpha- and beta-lysine, leucine, tyrosine, serine, aminoisobutyric acid, and beta-hydroxyvaline and its positional isomer. The separation was applicable to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase. However, for unknown reasons, no leucine 2,3-aminomutase activity could be found in rat liver homogenate.
Aberhart
D J
DJ
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
D000591:Amino Acid Isomerases / Q000032:analysis*; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D019751:Intramolecular Transferases; D007536:Isomerism; D008099:Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D000591:Amino Acid Isomerases; D019751:Intramolecular Transferases; C021518:lysine 2,3-aminomutase; C021507:leucine 2,3-aminomutase