3-Azi-1-methoxybutyl beta-D-galactopyranoside, a photoaffinity label for monoclonal antigalactan antibodies of the VH GAL 39.1/55.1 gene-family.
3-Azi-1-methoxybutyl beta-D-galactopyranoside, prepared from 2,3,4,6-tetra-O-acetyl-alpha-D-galactopyranosyl bromide in four steps, had an affinity constant for antigalactans IgA (Fab') J539 and X24 of 1550 and 1730 M-1, respectively. 3-Azi-1-methoxybutyl beta-D-(4-3H)galactopyranoside, when photolysed in the presence of IgA X24, specifically labelled the galactan-binding area of the immunoglobulin.
Jung
G L
GL
National Institutes of Health, Bethesda, MD 20892.
Holme
K R
KR
Glaudemans
C P
CP
Lehmann
J
J
Lay
H
H
D000345:Affinity Labels / Q000138:chemical synthesis*; D000911:Antibodies, Monoclonal; D005685:Galactans / Q000235:genetics* / Q000276:immunology; D005697:Galactosides / Q000138:chemical synthesis*; D006027:Glycosides / Q000138:chemical synthesis*; D007070:Immunoglobulin A; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D009904:Optical Rotation; D013050:Spectrometry, Fluorescence
D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D005685:Galactans; D005697:Galactosides; D006027:Glycosides; D007070:Immunoglobulin A; D008024:Ligands; C056090:3-azi-1-methoxybutylgalactopyranoside