Inhibitors of sterol synthesis. Reverse phase high performance liquid chromatography for the separation of cholesterol, 5 alpha-cholest-8(14)-en-3 beta-ol-15-one, and their fatty acid esters.
A relatively simple and rapid method was required for the separation of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one, a potent inhibitor of sterol synthesis, from its major metabolites. Conditions have been determined which permit the resolution of the 15-ketosterol and cholesterol and fatty acid esters of the two sterols by reverse phase high performance chromatography. This methodology also permits the resolution of the major esters of the 15-ketosterol and of cholesterol.
Chu
A
A
Department of Biochemistry, Rice University, Houston, TX 77251.
Schroepfer
G J
GJ
D002782:Cholestenes / Q000302:isolation & purification; D002783:Cholestenones / Q000302:isolation & purification; D002784:Cholesterol; D002788:Cholesterol Esters / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D010168:Palmitates / Q000302:isolation & purification; D014304:Triolein / Q000302:isolation & purification
D002782:Cholestenes; D002783:Cholestenones; D002788:Cholesterol Esters; D010168:Palmitates; D014304:Triolein; C014409:cholesteryl oleate; C015073:cholest-8(14)-en-3-ol-15-one; D002784:Cholesterol