title: Fertilization of human oocytes by sperm from infertile males after zona pellucida drilling.
abstract: Infertile couples who had failed to achieve fertilization of oocytes in previous trials of in vitro fertilization (IVF) were treated by IVF with zona pellucida drilling. Zona drilling entails use of micromanipulation to introduce a gap in the zona pellucida either mechanically or by localized application of a zona solvent from a microneedle. Ten couples were treated, from whom 63 oocytes were recovered for manipulation. Sixteen eggs were denuded of the cumulus oophorus only, and the remaining 47 eggs were subjected to zona drilling. Of the 16 eggs denuded but not drilled, 4 (25%) were fertilized. Of the 47 oocytes drilled, 31 survived (67%) and 10 of the surviving eggs (32%) were fertilized. The polyspermy rate for drilled eggs that fertilized was high (5/10, 50%), and polyspermic eggs were often penetrated by more than two spermatozoa. The remaining five eggs fertilized after drilling were diploid fertilizations, and in three cases cleavage was followed by embryo transfer, although pregnancies were not obtained. These data indicate that zona drilling has the potential for establishing pregnancies in instances where treatment by standard IVF would fail. In addition, results indicate that the block to polyspermy in human eggs occurs at the level of the zona pellucida.
authors: Department of Obstetrics, Gynecology and Reproductive Science, Mt. Sinai School of Medicine, New York, New York 10029.
authors: J W
authors: JW
authors: Gordon
authors: L
authors: L
authors: Grunfeld
authors: G J
authors: GJ
authors: Garrisi
authors: B E
authors: BE
authors: Talansky
authors: C
authors: C
authors: Richards
authors: N
authors: N
authors: Laufer
mesh_terms: D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007246:Infertility / Q000503:physiopathology*; D008297:Male; D010063:Ovum; D015044:Zona Pellucida