Simultaneous preparation from human placenta of several enzymes of glucose metabolism.
A procedure for the simultaneous purification to homogeneity of hexokinase, phosphoglucomutase 1 and 2, aldolase, phosphoglucose isomerase and glucose-6-phosphate dehydrogenase from human origin has been developed. Human placenta homogenate was first chromatographed on DE-52 column which retains hexokinase and glucose-6-phosphate dehydrogenase while the other enzymes are recovered in the unabsorbed protein fraction. The other steps in the purification involve Matrex gel and specific affinity chromatography for the DE-52 retained enzymes and phosphocellulose and Matrex gel chromatography for the other enzymes. All the enzymes mentioned were obtained in one week, with recoveries from 14 percent for glucose-6-phosphate dehydrogenase to 75 percent for hexokinase. Thus, the procedures utilized seem to be useful in obtaining large amounts of enzymes in a a homogeneous form from an easily available human tissue.
Fazi
A
A
Istituto di Chimica Biologica, Università degli Studi di Urbino, Italy.
Magnani
M
M
Accorsi
A
A
Ninfali
P
P
Fornaini
G
G
D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005947:Glucose / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000302:isolation & purification; D006593:Hexokinase / Q000302:isolation & purification; D006801:Humans; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D005947:Glucose