Renal microsomal cytochrome P-450 and the oxidative metabolism of arachidonic acid.
Arachidonic acid is oxidized by the microsomal cytochrome P-450 monooxygenases of rabbit kidney cortex and medulla to products that include W and W-1 alcohols and several epoxyacids. In vivo administration of deoxycorticosterone acetate results in a time dependent and selective induction of the arachidonic acid W/W-1 oxygenase activity of cortex and medulla microsomal fractions (145% and 220% of controls, respectively). Initial segmental analysis shows the presence of an active epoxygenase activity in the isolated rabbit pars recta and its in vitro activation by p-aminohippuric acid.
Lapuerta
L
L
Department of Biochemistry, University of Texas Health Science Center, Dallas.
Chacos
N
N
Falck
J R
JR
Jacobson
H
H
Capdevila
J H
JH
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000494:pharmacology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology*; D009399:Nephrons / Q000201:enzymology; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism*; D011817:Rabbits
D001095:Arachidonic Acids; D003900:Desoxycorticosterone; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D000090762:Cytochrome P-450 CYP2J2