Simultaneous liquid chromatographic screening of five coccidiostats in chicken liver.
A reverse-phase liquid chromatographic (LC) method is described for simultaneously determining 5 coccidiostats--aklomide, dinsed, ethopabate, nitromide, and zoalene in chicken liver. The method entails blender extraction of 10 g liver with ethyl acetate, column chromatography through Sephadex LH-20 and neutral alumina, and LC analysis on a C18 column with UV detection at 260 nm. The drugs were eluted from Sephadex with methanol-benzene (10 + 90), from alumina with methanol-dichloromethane (10 + 90), and from C18 with acetonitrile-water (linear gradient: 25% acetonitrile for 10 min, increasing to 55% over 15 min; flow rate 1 mL/min). Liquid chromatography was completed in 40 min and calculations were based on peak height measurements. Average recoveries of the coccidiostats from fortified liver ranged from 72 to 97%, except for dinsed, which showed a relatively constant average recovery of 57%. The detection limit for the standards was 2.5 ng on column. Levels as low as 50 ng/g were detected in fortified liver samples.
Gallicano
K D
KD
Can Test Ltd., Vancouver, Canada.
Park
H
H
Yee
J
J
Young
L M
LM
Saschenbrecker
P W
PW
D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008099:Liver / Q000032:analysis*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D003049:Coccidiostats; D012997:Solvents