title: Analysis of a common inheritable idiotype in IgA-deficient sera using monoclonal antibodies.
abstract: In these studies we describe the production of three mAb raised to an idiotype on an IgG anticasein antibody isolated from the serum of one IgA-deficient blood donor. These are IgM kappa and block the binding of casein Ag to anticasein antibody. Sera of unrelated IgA-deficient donors were tested for the presence of the idiotype; 15 of 56 IgA-deficient sera (25%) contain the anticasein idiotype, whereas 1 of 45 normal sera was positive. Anticasein antibodies as a whole were predominantly of the IgG1 and IgG3 subclass; idiotype-positive anticaseins are predominantly of the IgG1 subclass. For IgA-deficient donors, the relative amount of idiotype-positive anticasein antibody was correlated with the level of anticasein present in the serum. Studies were done to investigate the potential inheritance of the idiotype in families; in three of four families the idiotype was inherited in an apparent autosomal dominant pattern. Our data show that a common cross-reactive idiotype can be detected in the sera of IgA-deficient individuals and their family members. This suggests that V region markers may be conserved in this humoral immunodeficiency disease.
authors: Department of Medicine, Mount Sinai School of Medicine, New York 10029.
authors: C
authors: C
authors: Cunningham-Rundles
authors: Z K
authors: ZK
authors: Feng
mesh_terms: D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002364:Caseins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D005260:Female; D006801:Humans; D017098:IgA Deficiency; D007070:Immunoglobulin A / Q000235:genetics / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008297:Male; D010375:Pedigree
chemical_list: D000911:Antibodies, Monoclonal; D002364:Caseins; D007070:Immunoglobulin A; D007130:Immunoglobulin Idiotypes