Detection and use of salivary hemagglutinins for forensic blood grouping.
A sensitive method for the detection of anti-A and anti-B hemagglutinins in fresh saliva has been developed. The method utilizes a bromelin treated erythrocyte suspension as indicator cells and includes a simple procedure to concentrate these hemagglutinins. Antiserum directed against immunoglobulin A enhances the hemagglutination assay. We find that these salivary hemagglutinins are present in over 90% of the population and that their titer remains stable over a period of two months. These hemagglutinins can be used to blood type the donor of a saliva sample and can be used in a confirmatory test that complements the commonly used absorption-inhibition test which is used to detect salivary blood group agglutinogens. In preliminary studies we have determined that hemagglutinins can be successfully isolated and analyzed from dried saliva stains.
Kobilinsky
L
L
John Jay College of Criminal Justice, City University of New York, New York.
Harrington
J J
JJ
D000372:Agglutination Tests / Q000379:methods; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D006388:Hemagglutinins / Q000032:analysis*; D006801:Humans; D012463:Saliva / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis; D013696:Temperature
D006388:Hemagglutinins; D012471:Salivary Proteins and Peptides