title: Velocity of the creatine kinase reaction in the neonatal rabbit heart: role of mitochondrial creatine kinase.
abstract: To examine the role of changes in the distribution of the creatine kinase (CK) isoenzymes [BB, MB, MM, and mitochondrial CK (mito-CK)] on the creatine kinase reaction velocity in the intact heart, we measured the creatine kinase reaction velocity and substrate concentrations in hearts from neonatal rabbits at different stages of development. Between 3 and 18 days postpartum, total creatine kinase activity did not change, but the isoenzyme distribution and total creatine content changed. Hearts containing 0, 4, or 9% mito-CK activity were studied at three levels of cardiac performance: KCl arrest and Langendorff and isovolumic beating. The creatine kinase reaction velocity in the direction of MgATP production was measured with 31P magnetization transfer under steady-state conditions. Substrate concentrations were measured with 31P NMR (ATP and creatine phosphate) and conventional biochemical analysis (creatine) or estimated (ADP) by assuming creatine kinase equilibrium. The rate of ATP synthesis by oxidative phosphorylation was estimated with oxygen consumption measurements. These results define three relationships. First, the creatine kinase reaction velocity increased as mito-CK activity increased, suggesting that isoenzyme localization can alter reaction velocity. Second, the reaction velocity increased as the rate of ATP synthesis increased. Third, as predicted by the rate equation, reaction velocity increased with the 3-fold increase in creatine and creatine phosphate contents that occurred during development.
authors: Harvard Medical School NMR Laboratory, Department of Cardiology, Boston, Massachusetts 02115.
authors: S B
authors: SB
authors: Perry
authors: J
authors: J
authors: McAuliffe
authors: J A
authors: JA
authors: Balschi
authors: P R
authors: PR
authors: Hickey
authors: J S
authors: JS
authors: Ingwall
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D003402:Creatine Kinase / Q000378:metabolism*; D006321:Heart / Q000187:drug effects / Q000254:growth & development*; D007527:Isoenzymes; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008929:Mitochondria, Heart / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D011189:Potassium Chloride / Q000494:pharmacology; D011817:Rabbits
chemical_list: D007527:Isoenzymes; D011189:Potassium Chloride; D003402:Creatine Kinase