title: Extracellular oxidation of D-glucose by some members of the Enterobacteriaceae.
abstract: Extracellular D-glucose oxidation by 5 enterobacterial species was studied with the purpose of selecting conditions useful for taxonomic studies. Extracellular production of gluconate from 14C-glucose by bacterial cells was evidenced by DEAE-cellulose paper chromatography. Escherichia coli oxidized glucose only when pyrroloquinoline quinone (PQQ) was added, whereas Serratia marcescens, Yersinia frederiksenii, Erwinia cypripedii and Cedecea lapagei oxidized D-glucose without added PQQ. 2-Deoxyglucose was found to be an excellent non-metabolized analogue of D-glucose in oxidation experiments. D-glucose oxidation was inhibited by KCN, p-chloromercuribenzoic acid and carbonyl cyanide m-chlorophenylhydrazone; and activated by p-benzoquinone. Iodoacetate had no action. Comparative cellulose thin-layer chromatography including 2-ketogluconate and 2,5-diketogluconate (produced by Janthinobacterium lividum) as standards, showed that gluconate was oxidized to 2-ketogluconate by S. marcescens and E. cypripedii, and 2-ketogluconate was oxidized to 2,5-diketogluconate by E. cypripedii. The diversity of D-glucose oxidation products in the Enterobacteriaceae could have some taxonomic applications.
authors: Unité des Entérobactéries, Unité INSERM 199, Institut Pasteur, Paris.
authors: O M
authors: OM
authors: Bouvet
authors: P A
authors: PA
authors: Grimont
mesh_terms: D002241:Carbohydrates / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D004755:Enterobacteriaceae / Q000378:metabolism*; D005942:Gluconates / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000037:antagonists & inhibitors / Q000378:metabolism; D007700:Kinetics; D010084:Oxidation-Reduction; D045542:PQQ Cofactor; D011804:Quinolines / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D005942:Gluconates; D011804:Quinolines; D045542:PQQ Cofactor; D003847:Deoxyglucose; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D005947:Glucose; C030691:gluconic acid