High-performance liquid chromatography of biogenic amines, derivatized with 9-fluorenylmethyl chloroformate.
A procedure was elaborated for the analysis of three biogenic amines posing a considerable health hazard. The method takes advantage of the characteristics of the 9-fluorenylmethyl chloroformate (FMOC) derivative, namely specificity, stability and compatibility for either fluorescence or UV-absorbance detection. The FMOC-tyramine derivative was probably adsorbed to labware when acetone served as the solvent for FMOC. Methanol, substituted for acetone, removed this problem. Excellent linearity was obtained with standard solutions of tyramine, tryptamine and phenylethylamine. Meat samples, spiked with the mentioned amines, also showed good linearity. Perchloric acid was chosen for deproteinization, as potassium perchlorate may be eliminated on neutralization. Histamine failed to react with FMOC or was not detected under the test conditions.
Harduf
Z
Z
Department of Poultry Science, Volcani Center, Bet Dagan, Israel.
Nir
T
T
Juven
B J
BJ
D000818:Animals; D001679:Biogenic Amines / Q000302:isolation & purification*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes; D008460:Meat / Q000032:analysis*
D001679:Biogenic Amines; D005449:Fluorenes; C054007:1-(9-fluorenyl)methyl chloroformate