Microcalorimetric studies of hybridoma cells.
In the present study, overall metabolism has been estimated in hybridoma cells by microcalorimetric measurement. Heat production rate was found to be 30-50 pW/cell at cell concentrations 0.65-4.5 x 10(5)/ml. High cell concentrations (1 x 10(6) cells/ml) caused unstable power-curves with an initial high peak and a rapid declining phase, whereas low cell concentrations (0.6-4.5 x 10(5) cells/ml) produced steady-state power-curves. Oxygen consumption was found to range between 1.5-6.1 x 10(-5) mol 02/cell/min, corresponding to about 80% of the total metabolic activity. The metabolic inhibitors sodium fluoride (50 nM), sodium azide (160 mM) and rotenone (0.1 mM) caused a reduction in overall cell metabolism of 60, 55 and 40% respectively.
Nässberger
L
L
Research Department 1; University Hospital of Lund, Sweden.
Truedsson
L
L
Monti
M
M
D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001386:Azides / Q000494:pharmacology; D002151:Calorimetry; D002452:Cell Count; D002470:Cell Survival; D006825:Hybridomas / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption; D012402:Rotenone / Q000494:pharmacology; D019810:Sodium Azide; D012969:Sodium Fluoride / Q000494:pharmacology
D001386:Azides; D012402:Rotenone; D012969:Sodium Fluoride; D019810:Sodium Azide