Chemiluminescence immunoassay for progesterone in plasma incorporating acridinium ester labelled antigen.
A sensitive, solid-phase chemiluminescence immunoassay suitable for determining progesterone concentrations in plasma has been developed. The solid-phase antiserum was prepared by coupling a monoclonal progesterone-antibody, raised against a progesterone-11 alpha-hemisuccinyl/bovine serum albumin conjugate, to cyanogen bromide activated cellulose. An 11 alpha-progesteryl-2-carboxymethyltyramine-4-(10-methyl)-a cri dinium-9-carboxylate conjugate was used as the chemiluminescent label. The assay had a lower limit of sensitivity of 3 pg/assay tube and satisfied accepted validation criteria. Progesterone concentrations determined by chemiluminescence assay were in good agreement not only with a radioimmunoassay in routine use but also with a gas chromatography-mass spectrometry procedure.
Miller
S A
SA
Tenovus Institute for Cancer Research, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Morton
M S
MS
Turkes
A
A
D000166:Acridines; D000911:Antibodies, Monoclonal; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D011374:Progesterone / Q000031:analogs & derivatives* / Q000097:blood* / Q000276:immunology; D011863:Radioimmunoassay
D000166:Acridines; D000911:Antibodies, Monoclonal; C055464:11-progesteryl-2-carboxymethyltyramine-4-(10-methyl)acridinium-9-carboxylate; D011374:Progesterone