Determination of drug enantiomers in biological samples by coupled column liquid chromatography and liquid chromatography-mass spectrometry.
Liquid chromatography-mass spectrometry (LC-MS) and coupled column chromatography can be used to overcome problems likely to occur in direct separation and determination of drug enantiomers in biological samples. This is exemplified here with the direct separation and determination of terbutaline in human plasma at the nmol/l level. A beta-cyclodextrin column with an aqueous mobile phase was used for chiral separation. For coupled column chromatography, the concentration of each enantiomer was calculated from the enantiomeric area ratio and the racemate concentration. A deuterium-labelled internal standard was used in the LC-MS experiments.
Edholm
L E
LE
Bioanalysis, AB Draco, (Subsidiary of AB Astra), Lund, Sweden.
Lindberg
C
C
Paulson
J
J
Walhagen
A
A
D002849:Chromatography, Gas; D006801:Humans; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013237:Stereoisomerism; D013726:Terbutaline / Q000097:blood
D004364:Pharmaceutical Preparations; D013726:Terbutaline