Seeding with endothelial cells derived from the microvessels of the omentum and from the jugular vein: a comparative study.
Segments of an experimental polytetrafluoroethylene graft (9 cm long, 6 mm I.D.) of high porosity were implanted in 25 dogs as aortic interposition grafts. Nine grafts were seeded with a mean of 7 x 10(5) viable endothelial cells (ECs) derived from the jugular vein (group A) and eight were seeded with a mean of 7 x 10(5) viable ECs derived from the microvessels of the omentum (group B). Eight grafts were not seeded and they served as controls (group C). Animals were put to death 5 weeks after graft implantation. The thrombus-free area was measured at 81% +/- 10% in group A, 65% +/- 22% in group B, and 25% +/- 13% in group C (p less than 0.05, group A vs group B; p less than 0.05, group B vs group C). The thickness of the subendothelial layer was 151 +/- 60 microns in group A, 280 +/- 60 microns in group B, and 100 +/- 75 microns in group C (p less than 0.001, group B vs groups A and C). The production of 6-keto-PGF1 alpha in the presence of sodium arachidonate was higher in seeded grafts (p less than 0.05). Omentally derived microvessel ECs can be seeded in vascular grafts; refinements in the technique of EC procurement are required to minimize contamination and to obtain ECs with more effective biologic activity.
Sterpetti
A V
AV
Department of Surgery, Creighton University School of Medicine, Omaha, NE 68131.
Hunter
W J
WJ
Schultz
R D
RD
Sugimoto
J T
JT
Blair
E A
EA
Hacker
K
K
Chasan
P
P
Valentine
J
J
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000601:surgery; D001807:Blood Vessel Prosthesis / Q000009:adverse effects; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D007601:Jugular Veins / Q000166:cytology; D008833:Microcirculation / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D009852:Omentum / Q000098:blood supply; D011138:Polytetrafluoroethylene; D013927:Thrombosis / Q000517:prevention & control
D015121:6-Ketoprostaglandin F1 alpha; D011138:Polytetrafluoroethylene