Effect of pyruvate, octanoate and glucose on leucine degradation in skeletal muscle from fed and fasted chicks.
1. Pyruvate at 5 mM decreased the rate of leucine oxidative decarboxylation and increased the rate of 2-oxoisocaproate production in extensor digitorum communis (EDC) muscles from both fed and 24-hr fasted chicks. Pyruvate at 5 mM increased the net rate of leucine transamination in EDC muscle from fed chicks and had no effect in EDC muscles from 24-hr fasted chicks. 2. Octanoate at 0.2 and 1 mM markedly increased the rates of net leucine transamination, leucine oxidative decarboxylation and oxidation of decarboxylated leucine carbons 2-6 in EDC muscles from fed chicks, but had no effect on these parameters of leucine degradation in muscles from 24-hr fasted chicks. 3. Glucose at 5 and 12 mM decreased the rates of leucine oxidative decarboxylation and oxidation of decarboxylated leucine carbons 2-6, and increased the net rate of 2-oxoisocaproate production as compared to control (no glucose) group in muscles from fed chicks. Glucose had no effect on these parameters of leucine degradation in muscles from 24-hr fasted chicks.
Wu
G
G
Department of Animal Science, University of Alberta, Edmonton, Canada.
Thompson
J R
JR
D000818:Animals; D002210:Caprylates / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002645:Chickens; D004435:Eating; D005215:Fasting; D005947:Glucose / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011773:Pyruvates / Q000494:pharmacology*; D012016:Reference Values
D002210:Caprylates; D002250:Carbon Radioisotopes; D011773:Pyruvates; D007930:Leucine; D005947:Glucose