title: Interaction of embryonic chick calvarial bone cells with collagen substrata; attachment characteristics and growth behaviour.
abstract: Freshly isolated bone cells from embryonic chick calvariae were found to attach to films of native monomeric type I or II collagen, to gels of native type I collagen and, to a lesser extent, to air-dried films of these collagens, in a fibronectin-independent manner, and with no requirement for collagen synthesis. Adhesion to type III collagen was relatively poor. Films of native monomeric type I or II collagen were preferred to the corresponding air-dried films as substrata for proliferation. Cis-hydroxyproline inhibited growth upon both plastic and type I and type II collagenous substrata, but this effect could largely be overcome by providing bone cell-conditioned medium, suggesting the possibility of a requirement in the growth process for synthesis of a collagen other than type I, the major form in bone matrix.
authors: Strangeways Research Laboratory, Worts' Causeway, Cambridge, England.
authors: P M
authors: PM
authors: Royce
authors: M J
authors: MJ
authors: Barnes
mesh_terms: D000818:Animals; D001769:Blood; D001846:Bone Development; D001842:Bone and Bones / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis / Q000378:metabolism*; D005353:Fibronectins / Q000502:physiology; D006909:Hydroxyproline / Q000494:pharmacology
chemical_list: D005353:Fibronectins; D003094:Collagen; D006909:Hydroxyproline