title: Heterogenous inositol tetrakisphosphate binding sites in the adrenal cortex.
abstract: Bovine adrenal cortical microsomes possess high (Kd = 3.68 +/- 1.02 X 10(-9) M) and lower (Kd = 9.20 +/- 1.71 X 10(-8) M) affinity binding sites for inositol 1,3,4,5-tetrakisphosphate. The binding to these sites is rapid, saturable (reaches equilibrium by 15 min at 0 degrees C), and reversible. Competition studies with other inositol phosphate analogs indicate that the high affinity binding sites are clearly distinct from the inositol 1,4,5-trisphosphate receptors which are, however, responsible for a fraction of the lower affinity binding. The characteristics of the inositol 1,3,4,5-tetrakisphosphate binding sites described are compatible with their possible receptor function.
authors: Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
authors: P
authors: P
authors: Enyedi
authors: G H
authors: GH
authors: Williams
mesh_terms: D000302:Adrenal Cortex / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D007295:Inositol Phosphates / Q000378:metabolism*; D007536:Isomerism; D007700:Kinetics; D051381:Rats; D013403:Sugar Phosphates / Q000378:metabolism*
chemical_list: D007295:Inositol Phosphates; D013403:Sugar Phosphates; C081655:inositol-1,3,4,5-tetrakisphosphate