Distribution of acceptors for beta-bungarotoxin in the central nervous system of the rat.
High-affinity acceptors for 125I-beta-bungarotoxin have been identified on rat brain cryostat sections and mapped quantitatively using 3H-sensitive sheet film autoradiography. A unique distribution of acceptors has thus been observed; the toxin sites are particularly enriched in grey matter areas and synaptic regions, consistent with the pharmacological action of beta-bungarotoxin. As the binding was abolished by dendrotoxin, a related polypeptide known to inhibit fast-activating K+ conductances, the occurrence of beta-bungarotoxin acceptors may indicate the location of certain voltage-sensitive K+ channels. The overall distribution is, however, distinct from that of any other ion channel described.
Pelchen-Matthews
A
A
Department of Biochemistry, Imperial College of Science and Technology, London, U.K.
Dolly
J O
JO
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D002038:Bungarotoxins / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007700:Kinetics; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D002038:Bungarotoxins; D007457:Iodine Radioisotopes; D014316:Tritium