DNA-nitrosourea interactions. High-performance liquid chromatography of cross-linked dinucleosides and substituted deoxynucleosides.
A reconstituted mixture of five cross-linked dinucleosides possibly involved in DNA-nitrosourea interactions, and of their degradation products (nucleobases, deoxynucleosides and mono- or disubstituted deoxynucleosides), was analysed by reversed-phase high-performance liquid chromatography using C18 columns and an diode-array detector. The chromatographic conditions for separating the twenty-one investigated compounds were optimized, and the compounds were identified by both their retention times and their UV spectra. A structure-retention time relationship was observed under suitable conditions and is discussed. Its validity was confirmed by the prediction of the retention time of a new cross-linked dinucleoside synthesized for this purpose.
Maggio
A F
AF
Laboratoire de Chimie Bio-Organique, Université des Sciences, Montpellier, France.
Pompon
A
A
Lucas
M
M
Barascut
J L
JL
Imbach
J L
JL
D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009607:Nitrosourea Compounds / Q000032:analysis*; D009705:Nucleosides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D009607:Nitrosourea Compounds; D009705:Nucleosides; D004247:DNA