Synthesis of [gamma-32P]thiamine triphosphate.
We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%.
Grandfils
C
C
Laboratoire de Biochimie Générale et Comparée, Université de Liège, Belgium.
Bettendorff
L
L
de Rycker
C
C
Schoffeniels
E
E
D002852:Chromatography, Ion Exchange; D007553:Isotope Labeling / Q000379:methods*; D010761:Phosphorus Radioisotopes; D013831:Thiamine / Q000031:analogs & derivatives*; D013838:Thiamine Triphosphate / Q000138:chemical synthesis* / Q000302:isolation & purification
D010761:Phosphorus Radioisotopes; D013838:Thiamine Triphosphate; D013831:Thiamine