Effect of oleate on the apparent Km of monoamine oxidase and the amount of membrane-bound hexokinase in isolated rat hepatocytes: further evidence for the controlling role of the surface charge in hexokinase binding.
Brief incubation of isolated rat hepatocytes in the presence of the oleate-bovine serum albumin complex resulted in a release to the cytosol of a portion of hexokinase (EC 2.7.1.1) normally bound to intracellular membranes. This was correlated with an increase of the negative surface potential of the outer mitochondrial membrane, as measured in situ by determining changes of Km of monoamine oxidase (EC 1.4.3.4). It is suggested that non-esterified fatty acids produce a partial release of bound hexokinase in the liver cell by changing the surface charge of intracellular membranes.
Wojtczak
L
L
Nencki Institute of Experimental Biology, Warsaw, Poland.
Adams
V
V
Brdiczka
D
D
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006593:Hexokinase / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000494:pharmacology
D009829:Oleic Acids; D012709:Serum Albumin; D019301:Oleic Acid; D007770:L-Lactate Dehydrogenase; D008995:Monoamine Oxidase; D006593:Hexokinase