Immunohistological diagnosis of primary brain lymphoma using monoclonal antibodies: confirmation of B-cell origin.
The antigenic characteristics of 20 primary cerebral lymphomas have been defined by their reactivity with a panel of monoclonal antibodies recognizing differentiation antigens of lymphocytes and other cell types. In 7 out of 20 cases (35%), immunohistological results were diagnostically crucial and this approach appeared almost to double the detection rate of brain lymphomas over a 10-year period. All 20 tumours were confirmed as B-cell neoplasms by the use of a monoclonal antibody (B-1) specific for B-lymphocytes, rather than by the demonstration of immunoglobulin production. Further immunophenotyping with antibody FMC7 indicated that the neoplastic B-cells had been 'arrested' at a relatively mature stage of differentiation. The importance of monoclonal antibody markers in the accurate diagnosis and characterization of primary cerebral lymphomas has now been established.
Garson
J A
JA
Imperial Cancer Research Fund Brain Tumour Group, Frenchay Hospital, Bristol.
Bourne
S P
SP
Allan
P M
PM
Leather
C
C
Brownell
D B
DB
Coakham
H B
HB
D000368:Aged; D000911:Antibodies, Monoclonal; D001932:Brain Neoplasms / Q000175:diagnosis / Q000000981:diagnostic imaging / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000175:diagnosis / Q000000981:diagnostic imaging / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011859:Radiography
D000911:Antibodies, Monoclonal