Nephrotoxicity of uranyl fluoride in uninephrectomized and sham-operated rats.
The aim of the present study was to determine whether the nephrotoxicity of the uranium-containing compound uranyl fluoride (UO2F2) is enhanced after unilateral nephrectomy. Unilaterally nephrectomized (NPX) and sham-operated (SO) rats were given single intravenous injections of UO2F2 at doses delivering 100 or 250 micrograms U/kg 16 days after surgery. Between the second and third day after the administration of either dose of UO2F2, the urinary excretion of the cellular enzymes lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) and the plasma solute albumin began to increase significantly in both the NPX and SO rats. The urinary excretion of the plasma solute glucose did not begin to increase significantly in the NPX and SO rats until 4 days after the administration of either dose of UO2F2. During the fifth day following the administration of either dose of UO2F2 (which was also the last day that urinary data were collected) the urinary excretion of LDH, AST, and glucose in the NPX and SO rats was greater than that during any previous day. The urinary excretion of these three compounds during this fifth day was greater in the SO rats than in the NPX rats. Also during the fifth day following the injection of either dose of UO2F2, the fractional excretion of glucose was higher in the SO rats than in the NPX rats. By the end of the fifth day, the level of histologically demonstrable cellular necrosis in the pars recta of proximal tubules in the renal cortex and outer medulla of the NPX and SO rats was statistically similar. Therefore, the nephropathy in rats induced by UO2F2 is not made more severe as a result of unilateral nephrectomy.
Zalups
R K
RK
Department of Pharmacology, University of Rochester Medical Center, New York 14642.
Gelein
R M
RM
Morrow
P E
PE
Diamond
G L
GL
D000410:Alanine Transaminase / Q000652:urine; D000419:Albuminuria; D000818:Animals; D003404:Creatinine / Q000493:pharmacokinetics; D005459:Fluorides; D005947:Glucose / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000652:urine; D008297:Male; D009392:Nephrectomy; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D014501:Uranium / Q000378:metabolism / Q000633:toxicity*; D017974:Uranium Compounds
D017974:Uranium Compounds; C036637:uranyl fluoride; D014501:Uranium; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase; D005947:Glucose; D005459:Fluorides