Topological distribution of choline phospholipid fatty acids in trout intestinal brush-border membrane.
The transbilayer distribution of choline phospholipids in trout intestinal brush-border membrane has been investigated using phospholipase C (from Clostridium welchii). In the middle intestine, 84% of phosphatidylcholine (PC) and 60% of sphingomyelin (SP) are located in the outer membrane leaflet. In the posterior intestine, 89% of PC and 52% of SP are located in the outer membrane leaflet. The externally located PC molecular species are (n - 3) fatty acid-rich in both parts of the intestine. While the sphingomyelin molecular species containing 24:1(n - 9) are exclusively located in the outer leaflet in the middle intestine, those containing 14:0 are more abundant in the same leaflet but in the posterior intestine. This strongly asymmetric distribution of both choline phospholipids may have numerous consequences on the brush-border membrane characteristics.
Pelletier
X
X
Centre de Neurochimie du CNRS, Strasbourg, France.
Freysz
L
L
Leray
C
C
D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D008871:Microvilli / Q000648:ultrastructure; D010713:Phosphatidylcholines / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism*; D014337:Trout
D005227:Fatty Acids; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins; D010740:Phospholipases