The identification and characterization of a GABAergic system in the cholinergic neuroblastoma x glioma hybrid clone NG108-15.
gamma-Aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD) activity were identified and characterized in the cholinergic neuronal cell clone NG108-15. GAD activity is similar to form A in rat brain by being temperature sensitive and independent of pyridoxal 5'-phosphate in the assay mixture. Most of the NG108-15 GAD activity is inactivated by 1 mM amino-oxyacetic acid. In contrast to NG108-15 choline acetyltransferase, GAD activity is not enhanced when cells are grown with dibutyryl cyclic AMP. The GAD activity shows a cell-density dependent increase that does not correlate with changing level of endogenous GABA. The identification of GABAergic properties in the NG108-15 clone enhances its use as a neuronal model for studies of differentiation and neurotoxicity of drugs or chemicals.
Searles
C D
CD
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Singer
H S
HS
D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002799:Cholinergic Fibers / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005910:Glioma / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D006822:Hybrid Cells / Q000378:metabolism*; D007700:Kinetics; D009447:Neuroblastoma / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D004791:Enzyme Inhibitors; D000625:Aminooxyacetic Acid; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase