Effects of aminopeptidase inhibitors actinonin and amastatin on chemotactic and phagocytic responses of human neutrophils.
Actinonin and amastatin are low-molecular-weight inhibitors of aminopeptidases associated with cell surfaces. The purpose of this study was to determine their effects on human neutrophil functions such as chemotaxis and phagocytosis. Both actinonin and amastatin enhanced chemotaxis to the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine. On the other hand, the effects of both agents on neutrophil phagocytosis were varied. Bacterial attachment to neutrophils was slightly affected by these agents. However, actinonin enhanced the internalization of bacteria by neutrophils. Neutrophil leucine aminopeptidase activity was also determined and was found to be weakly inhibited by these agents.
Matsuda
N
N
Biochemical Research Laboratory, Sunstar Inc., Osaka, Japan.
Katsuragi
Y
Y
Saiga
Y
Y
Tanaka
T
T
Nakamura
M
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology; D010455:Peptides; D010587:Phagocytosis / Q000187:drug effects*; D012044:Regression Analysis
D000900:Anti-Bacterial Agents; D006877:Hydroxamic Acids; D009842:Oligopeptides; D010455:Peptides; C016977:amastatin; C010531:actinonin