Number of tRNA binding sites on 80 S ribosomes and their subunits.
The ability of rabbit liver ribosomes and their subunits to form complexes with different forms of tRNAPhe (aminoacyl-, peptidyl- and deacylated) was studied using the nitrocellulose membrane filtration technique. The 80 S ribosomes were shown to have two binding sites for aminoacyl- or peptidyl-tRNA and three binding sites for deacylated tRNA. The number of tRNA binding sites on 80 S ribosomes or 40 S subunits is constant at different Mg2+ concentrations (5-20 mM). Double reciprocal or Scatchard plot analysis indicates that the binding of Ac-Phe-tRNAPhe to the ribosomal sites is a cooperative process. The third site on the 80 S ribosome is formed by its 60 S subunit, which was shown to have one codon-independent binding site specific for deacylated tRNA.
Rodnina
M V
MV
Institute of Molecular Biology and Genetics, USSR Academy of Sciences, Kiev.
El'skaya
A V
AV
Semenkov YuP
Kirillov
S V
SV
D000818:Animals; D007700:Kinetics; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism*; D011817:Rabbits; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure
D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe