title: A variance formula for gene mutation studies using agar plates.
abstract: Previous attempts to derive a formula for the variance of the estimated mutant fraction have assumed (without providing justification) that variance components due to treatment, growth and dilution, and plating, are additive. We have derived a variance formula that does not depend on this assumption. Our formula also includes variability attributable to imprecise counting of cells to be plated in the non-selective conditions.
authors: SRI International, Menlo Park, California 94025.
authors: H S
authors: HS
authors: Javitz
mesh_terms: D000362:Agar; D000704:Analysis of Variance; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D009154:Mutation
chemical_list: D000362:Agar