Sequence variability and function of measles virus 3' and 5' ends and intercistronic regions.
Sequences critical for the activity of the measles virus (MV) RNA polymerase in transcription and replication were analyzed using a MV genomic cDNA library containing overlapping clones encompassing the entire MV genome. Clones corresponding to the 3' and 5' ends of the MV genome were identified and sequenced, and these sequences were confirmed by primer extension experiments. Neither (+) nor (-) strand leader RNAs were detected in MV-infected cell extracts, using high specific activity riboprobes made form these clones. Clones representing each of the MV gene boundaries were also sequenced, and variations including point mutations, insertions, and deletions were noted. Together with the sequence of the MV L gene region, this report completes the sequence determination of the MV genome.
Crowley
J C
JC
Neurology Service, East Orange VA Medical Center, New Jersey 07019.
Dowling
P C
PC
Menonna
J
J
Silverman
J I
JI
Schuback
D
D
Cook
S D
SD
Blumberg
B M
BM
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D006860:Hydrogen Bonding; D008459:Measles virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000235:genetics*
D012367:RNA, Viral; D004247:DNA