Rhodoquinone requirement of the Hymenolepis diminuta mitochondrial electron transport system.
The occurrence of rhodoquinone as a mitochondrial membrane component was demonstrated in adult Hymenolepis diminuta. Chromatographic separation of pentane extracts, from lyophilized mitochondrial membranes, coupled with spectral analyses of separated material demonstrated the presence of rhodoquinone. The presence of ubiquinone was not apparent. Rhodoquinone content of membranes was about 1.2 micrograms (mg protein)-1. The rhodoquinone requirement of the H. diminuta electron transport system was demonstrated both in terms of the less active NADH oxidase and the physiologically required, NADH-dependent fumarate reductase employing lyophilized mitochondrial membranes as the source of activities. Pentane extraction of membranes virtually abolished the oxidase and fumarate reductase systems. Supplementation of pentane-treated membranes with H. diminuta rhodoquinone restored oxidase and fumarate reductase activities to levels simulating those of lyophilized membranes. Ubiquinone did not substitute for rhodoquinone. The rhodoquinone-reconstituted membranes displayed rotenone sensitivity. These findings represent the first direct demonstration of the rhodoquinone requirement of helminth electron transport-coupled oxidase and fumarate reductase.
Fioravanti
C F
CF
Department of Biological Sciences, Bowling Green State University, OH 43403.
Kim
Y
Y
D000818:Animals; D004579:Electron Transport; D006926:Hymenolepis / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*; D007425:Intracellular Membranes / Q000032:analysis / Q000201:enzymology / Q000378:metabolism; D008928:Mitochondria / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010101:Oxygen Consumption; D010545:Peroxides / Q000378:metabolism; D013386:Succinates / Q000096:biosynthesis; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
D009097:Multienzyme Complexes; D010545:Peroxides; D013386:Succinates; D014451:Ubiquinone; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C020155:fumarate reductase (NADH); C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; C003304:rhodoquinone