title: Modification of collagen and noncollagenous proteins in radiation-induced muscular fibrosis.
abstract: Six months after acute local gamma irradiation of the pig skin and adjacent muscle, the muscular tissue is replaced by a large mutilating and proliferative fibrosis deliminated by a perifibrotic inflammatory zone. The content and biosynthesis of collagen and noncollagenous proteins were studied in both fibrotic and perifibrotic zones after incubation of the biopsies with [14C]proline or [35S]methionine for 24 hr. Cells of perifibrotic and fibrotic regions synthesize about 10 times more proteins than those in the nonirradiated muscle. When compared to normal muscle tissue, our results indicate an important increase in collagen content and biosynthesis in fibrotic tissue. The increase in collagen biosynthesis in the irradiated tissue is more pronounced for type III collagen than for type I collagen. Biosynthesis of type III and type I collagens increases 20- and 10-fold, respectively, compared to the normal muscle. Type I to III collagen ratio in irradiated tissue decreases from 2.3 in normal tissue to 1.1 in fibrotic tissue. Histological examination of the biopsies as well as the protein pattern by polyacrylamide gel electrophoresis show striking differences in the perifibrotic and fibrotic areas as compared to the normal muscular tissue with a progressive disappearance of the myotubes replaced by a dense sclerotic tissue. The results indicate that the perifibrotic inflammatory area is engaged in a remodeling process and that the fibrotic tissue remains active in the neosynthesis of the extracellular matrix macromolecules with a high proportion of type III collagen. This high biosynthetic activity of the irradiated tissue may explain the pseudosarcomatous character of the radiation-induced lesions.
authors: Laboratoire de Biochimie du tissu Conjonctif, UA CNRS 1174, Faculté de Médecine, Université Paris Val de Marne, Créteil, France.
authors: J
authors: J
authors: Wegrowski
authors: C
authors: C
authors: Lafuma
authors: J L
authors: JL
authors: Lefaix
authors: F
authors: F
authors: Daburon
authors: L
authors: L
authors: Robert
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D005355:Fibrosis; D005720:Gamma Rays / Q000009:adverse effects; D007249:Inflammation; D008297:Male; D009135:Muscular Diseases / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D011506:Proteins / Q000378:metabolism*; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D013552:Swine
chemical_list: D011506:Proteins; D003094:Collagen