Extra- and intracellular hydrogen ion-selective microelectrode based on neutral carriers with extended pH response range in acid media.
A series of new neutral hydrogen ion carriers suitable for application in H+-selective microelectrodes is presented. One carrier (ETH 1907) proves to be superior to tridodecylamine currently very much in use. Microelectrodes based on ETH 1907 in an optimized membrane composition exhibit a linear dynamic response function from pH 2 to 9 extended into the acidic range, a response time less than or equal to 5 s, and a resistance of about 35 G omega for a tip diameter of about 1 micron. This makes the electrode suitable for measurements at normal physiological intracellular pH as well as in acid physiological media. Measurements using this microelectrode in proximal tubule cells of isolated perfused frog kidney are presented.
Chao
P
P
Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule Zürich (ETH), Switzerland.
Ammann
D
D
Oesch
U
U
Simon
W
W
Lang
F
F
D000818:Animals; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D008722:Methods; D008839:Microelectrodes; D011898:Ranidae
D007202:Indicators and Reagents