Evidence for the occurrence of LDL receptors in extracts of schistosomula of Schistosoma mansoni.
Schistosomula of Schistosoma mansoni were shown to contain proteins on their surface membranes which bind iodinated human low density lipoproteins (125I-LDL). Treatment of the parasites with trypsin decreased the binding in comparison with untreated controls. Membrane-bound, acetone-insoluble proteins were extracted from the schistosomula with Triton X-100 and the extract in liposome form was incubated with 125I-LDL at room temperature. After incubation a complex was formed between the proteins present in the extract and 125I-LDL, as shown by a filter binding assay. 125I-LDL binding to filters was proportional to the amount of protein in the extract; it was inhibited by unlabelled LDL and VLDL and by EDTA. Binding of 125I-LDL to proteins present in the liposome suspension containing the Triton X-100 extract followed saturation kinetics, indicating the occurrence of receptors for lipoproteins in the extract.
Rumjanek
F D
FD
Departamento de Bioquímica, Universidade Federal de Minas Gerais, Pampulha, Belo Horizonte, Brazil.
Campos
E G
EG
Afonso
L C
LC
D000818:Animals; D007700:Kinetics; D011973:Receptors, LDL / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D012550:Schistosoma mansoni / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
D011973:Receptors, LDL; D014357:Trypsin