title: Synthetic beta-turn peptides as substrates for a tyrosine protein kinase.
abstract: An attempt has been made at defining the secondary structural requirement for phosphorylation of substrates of a protein tyrosine kinase from the leukemia virus-transformed LSTRA cell line. An examination of the sites of phosphorylation of substrates of protein tyrosine kinases indicated a relatively high probability of the beta-turn as the secondary structural feature at these sites. We have, therefore, synthesized three tyrosine peptides: Ala-Pro-Tyr-Gly-NHCH3, Leu-Pro-Tyr-Ala-NHCH3, and Pro-Gly-Ala-Tyr-NH2, of which the first two peptides, but not the third, would be expected to contain the tyrosine residue in a beta-turn. Circular dichroism and infrared spectral data on the peptides confirmed this expectation. Phosphorylation data on the peptides by the tyrosine kinase showed that the two beta-turn peptides were phosphorylated with Vmax and Km values comparable to those of the 13-residue-long arginine-containing synthetic peptide substrate having a sequence homologous to the autophosphorylation site of the LSTRA kinase. The peptides used here contain the shortest sequence length among the reported synthetic peptide substrates for protein tyrosine kinases. Their preference for the beta-turn indicated that this conformation may serve as the recognition site for tyrosine phosphorylation.
authors: Department of Pharmacology, University of Washington, Seattle 98195.
authors: D A
authors: DA
authors: Tinker
authors: E A
authors: EA
authors: Krebs
authors: I C
authors: IC
authors: Feltham
authors: S K
authors: SK
authors: Attah-Poku
authors: V S
authors: VS
authors: Ananthanarayanan
mesh_terms: D002472:Cell Transformation, Viral; D002942:Circular Dichroism; D007700:Kinetics; D008979:Moloney murine leukemia virus; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D010766:Phosphorylation; D011487:Protein Conformation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013055:Spectrophotometry, Infrared
chemical_list: D010455:Peptides; D011505:Protein-Tyrosine Kinases