title: Specific and stable labeling of antibodies with technetium-99m with a diamide dithiolate chelating agent.
abstract: Technetium-99m labeling of antibodies has been suboptimal because of low affinity adventitious binding, nonspecific labeling, and loss of immunoreactivity. The diamide dithiolate ligand system (N2S2) forms highly stable, well-defined tetradentate complexes with Tc(V). Antibodies and their fragments have been labeled by conjugation of preformed 99mTc-4,5-bis(thioacetamido)pentanoate active ester to protein amine groups to give a chemically known 99mTc-N2S2 complex covalently linked to antibody. Evaluations of the 99mTc-N2S2-bound antibodies and their fragments have shown high stability and retained immunoreactivity.
authors: NeoRx Corporation, Seattle, WA 98119.
authors: A R
authors: AR
authors: Fritzberg
authors: P G
authors: PG
authors: Abrams
authors: P L
authors: PL
authors: Beaumier
authors: S
authors: S
authors: Kasina
authors: A C
authors: AC
authors: Morgan
authors: T N
authors: TN
authors: Rao
authors: J M
authors: JM
authors: Reno
authors: J A
authors: JA
authors: Sanderson
authors: A
authors: A
authors: Srinivasan
authors: D S
authors: DS
authors: Wilbur
mesh_terms: D000818:Animals; D000906:Antibodies; D000912:Antibodies, Neoplasm; D001391:Azo Compounds; D002614:Chelating Agents; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003958:Diamide; D007140:Immunoglobulin Fab Fragments; D008024:Ligands; D008546:Melanoma, Experimental / Q000000981:diagnostic imaging; D051379:Mice; D011877:Radionuclide Imaging / Q000379:methods*; D013667:Technetium
chemical_list: D000906:Antibodies; D000912:Antibodies, Neoplasm; D001391:Azo Compounds; D002614:Chelating Agents; D007140:Immunoglobulin Fab Fragments; D008024:Ligands; D003958:Diamide; D013667:Technetium