title: Parasite antigens are present in breast milk of women infected with Onchocerca volvulus.
abstract: We used a noncompetitive two-site ELISA with 5 monoclonal antibodies to determine whether parasite antigens are present in breast milk from women infected with Onchocerca volvulus. Seven out of 13 available milk samples contained significant amounts of filarial antigens. Antigen indices in milk correlated with levels of microfilarodermia (Rs = 0.74, P less than 0.005). Antigen-containing milk samples markedly inhibited mitogen-induced proliferation of human mononuclear cells and activated cells within this population that suppressed the proliferative response of autologous lymphocytes to mitogens and antigens. These findings indicate that parasite products are present in breast milk of O. volvulus-infected women and suggest that these may induce immune tolerance and/or suppression in infants born of infected mothers.
authors: Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.
authors: I
authors: I
authors: Petralanda
authors: L
authors: L
authors: Yarzabal
authors: W F
authors: WF
authors: Piessens
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D008213:Lymphocyte Activation; D008895:Milk, Human / Q000276:immunology*; D009854:Onchocerca / Q000276:immunology*; D009855:Onchocerciasis / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth