Analysis of vinca alkaloids in plasma and urine using high-performance liquid chromatography with electrochemical detection.
The reversed-phase high-performance liquid chromatography with electrochemical detection was used to quantify plasma and urine levels of vinblastine, vincristine, vindesine and a metabolite of vinblastine, desacetylvinblastine. Sample clean-up consisted of solid-phase extraction with a Bond Elut CN column. The extracts were separated on a Hypersil ODS column. The mobile phase consisted of a mixture of methanol and 10 mM phosphate buffer (pH 7.0). The limit of sensitivity using electrochemical detection was 100 pg on-column for all compounds with a signal-to-noise ratio of 3. Quantification of the compounds in human plasma and urine was possible down to 1 ng/ml (ca. 1 pmol). Pharmacokinetic results show that the sensitivity of the method is adequate for drug monitoring in clinical research.
Vendrig
D E
DE
Department of Pharmaceutical Analysis, University of Utrecht, The Netherlands.
Teeuwsen
J
J
Holthuis
J J
JJ
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D014747:Vinblastine / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D014748:Vinca Alkaloids / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D014750:Vincristine / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine
D014748:Vinca Alkaloids; D014750:Vincristine; D014747:Vinblastine