Structure of the horseradish peroxidase isozyme C genes.
We have isolated, cloned and characterized three cDNAs and two genomic DNAs corresponding to the mRNAs and genes for the horseradish (Armoracia rusticana) peroxidase isoenzyme C (HPR C). The amino acid sequence of HRP C1, deduced from the nucleotide sequence of one of the cDNA clone, pSK1, contained the same primary sequence as that of the purified enzyme established by Welinder [FEBS Lett. 72, 19-23 (1976)] with additional sequences at the N and C terminal. All three inserts in the cDNA clones, pSK1, pSK2 and pSK3, coded the same size of peptide (308 amino acid residues) if these are processed in the same way, and the amino acid sequence were homologous to each other by 91-94%. Functional amino acids, including His40, His170, Tyr185 and Arg183 and S-S-bond-forming Cys, were conserved in the three isozymes, but a few N-glycosylation sites were not the same. Two HRP C isoenzyme genomic genes, prxC1 and prxC2, were tandem on the chromosomal DNA and each gene consisted of four exons and three introns. The positions in the exons interrupted by introns were the same in two genes. We observed a putative promoter sequence 5' upstream and a poly(A) signal 3' downstream in both genes. The gene product of prxC1 might be processed with a signal sequence of 30 amino acid residues at the N terminus and a peptide consisting of 15 amino acid residues at the C terminus.
Fujiyama
K
K
Department of Fermentation Technology, Osaka University, Japan.
Takemura
H
H
Shibayama
S
S
Kobayashi
K
K
Choi
J K
JK
Shinmyo
A
A
Takano
M
M
Yamada
Y
Y
Okada
H
H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D005796:Genes; D006735:Horseradish Peroxidase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification
D000596:Amino Acids; D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D006735:Horseradish Peroxidase; D010544:Peroxidases