Chronic granulomatous disease with neutrophil membrane cytochrome b deficiency: demonstration by immunochemical staining with monoclonal antibody.
Cytochrome b deficiency in the peripheral granulocytes of two male patients with chronic granulomatous disease was demonstrated by an immunocytochemical assay using a monoclonal antibody, 7D5, against human neutrophil cytochrome b. A mosaic of cytochrome b positive and negative neutrophils, indicating a carrier state in an X-linked trait, was found in the mother of patient 1 but not in the mother of patient 2.
Minegishi
N
N
Department of Pediatrics, Tohoku University, Sendai.
Nakamura
M
M
Suzaki
K
K
Terasawa
M
M
Minegishi
M
M
Tsuchiya
S
S
Konno
T
T
D000911:Antibodies, Monoclonal; D002648:Child; D003573:Cytochrome b Group / Q000097:blood / Q000172:deficiency* / Q000276:immunology; D005260:Female; D008040:Genetic Linkage; D006105:Granulomatous Disease, Chronic / Q000097:blood* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D014960:X Chromosome
D000911:Antibodies, Monoclonal; D003573:Cytochrome b Group