title: [Effect of N4-methylcytosine and 5-methylcytosine on the stability of the DNA helix].
abstract: The thermodynamic parameters (delta H, delta S) of the helix-coil transition of self-complementary oligonucleotides d(CGCGCGCG), d(CG5mCGCGCG), d(CG4mCGCGCG), d(GGACCCGGGTCC), d(GGA5mCCCGGGTCC), and d(GGA4mCCCGGGTCC) were determined. The substitution of 4mC for C was found to decrease the melting temperature of the oligonucleotides. The destabilization effect of the two substitutions is equivalent to the change of A.T for G.C pair. The free energy decrease of the helix-coil transition due to the introduction of two 4mC into an octanucleotide was estimated to be 1,24 kcal/mol.
authors: A P
authors: AP
authors: Iurgaĭtis
authors: V V
authors: VV
authors: Butkus
authors: S I
authors: SI
authors: Klimashauskas
authors: A A
authors: AA
authors: Ianulaĭtis
mesh_terms: D044503:5-Methylcytosine; D003596:Cytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism; D008745:Methylation; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
chemical_list: D003854:Deoxyribonucleotides; C039052:N(4)-methylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA
vernacular_title: Vliianie N4-metiltsitozina i 5-metiltsitozina na stabil'nost' spirali DNK.