title: 3-Azi-1-methoxybutyl beta-D-galactopyranoside, a photoaffinity label for monoclonal antigalactan antibodies of the VH GAL 39.1/55.1 gene-family.
abstract: 3-Azi-1-methoxybutyl beta-D-galactopyranoside, prepared from 2,3,4,6-tetra-O-acetyl-alpha-D-galactopyranosyl bromide in four steps, had an affinity constant for antigalactans IgA (Fab') J539 and X24 of 1550 and 1730 M-1, respectively. 3-Azi-1-methoxybutyl beta-D-(4-3H)galactopyranoside, when photolysed in the presence of IgA X24, specifically labelled the galactan-binding area of the immunoglobulin.
authors: National Institutes of Health, Bethesda, MD 20892.
authors: G L
authors: GL
authors: Jung
authors: K R
authors: KR
authors: Holme
authors: C P
authors: CP
authors: Glaudemans
authors: J
authors: J
authors: Lehmann
authors: H
authors: H
authors: Lay
mesh_terms: D000345:Affinity Labels / Q000138:chemical synthesis*; D000911:Antibodies, Monoclonal; D005685:Galactans / Q000235:genetics* / Q000276:immunology; D005697:Galactosides / Q000138:chemical synthesis*; D006027:Glycosides / Q000138:chemical synthesis*; D007070:Immunoglobulin A; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D009904:Optical Rotation; D013050:Spectrometry, Fluorescence
chemical_list: D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D005685:Galactans; D005697:Galactosides; D006027:Glycosides; D007070:Immunoglobulin A; D008024:Ligands; C056090:3-azi-1-methoxybutylgalactopyranoside