[Effect of cycloheximide on the morphological and biochemical changes in chromatin in non-irradiated and irradiated rat thymocytes].
The level of chromatin degradation was studied and the method of electron-microscopy was used to estimate the changes in the ultrastructure of irradiated and nonirradiated thymocytes of rats treated with cycloheximide. The latter was found to decrease the degree of derangement of nuclear ultrastructure and the level of chromatin degradation in exposed animals and to increase the yield of these damages in thymocytes of nonirradiated animals. The electronmicroscopic determinations showed that the percentage of thymocytes with the impaired nucleus structure is twice as high as that of degraded chromatin. The causes of the quantitative disagreement between the morphological and biochemical indices of the interphase thymocyte death are discussed.
Aleksandrova
S E
SE
Ivannik
B P
BP
Gulaev
V A
VA
Riabchenko
N I
NI
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002843:Chromatin / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D003037:Cobalt Radioisotopes; D003513:Cycloheximide / Q000494:pharmacology*; D005720:Gamma Rays; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D013950:Thymus Gland / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure
D002843:Chromatin; D003037:Cobalt Radioisotopes; D003513:Cycloheximide
Vliianie tsiklogeksimida na morfologicheskie i biokhimicheskie izmeneniia khromatina v neobluchennykh i obluchennykh timotsitov krys.