Temperature-sensitive v-sea transformed erythroblasts: a model system to study gene expression during erythroid differentiation.
The isolation and characterization of a temperature-sensitive mutant (ts1 S13) of the avian erythroblastosis virus, S13, is described. The temperature-sensitive lesion in ts1 S13 was identified as affecting the tyrosine kinase activity but not the plasma membrane localization of the ts1 S13 v-sea gene product. Erythroblasts transformed by ts1 S13 can be induced to synchronously differentiate into erythrocytes in an erythropoietin (EPO)-dependent fashion. Analysis of erythrocyte-specific gene expression in ts1 S13 erythroblasts reveals that the transformed, self-renewing erythroblasts obtained at permissive temperature already express all erythrocyte genes tested for, although at a low level. Upon differentiation induction, expression of erythrocyte-specific genes is not coordinately regulated but rather involves complex regulatory mechanisms that appear to be specific for the individual genes.
Knight
J
J
State University of New York, Department of Microbiology, Stony Brook 11790.
Zenke
M
M
Disela
C
C
Kowenz
E
E
Vogt
P
P
Engel
J D
JD
Hayman
M J
MJ
Beug
H
H
D001355:Alpharetrovirus / Q000235:genetics; D000818:Animals; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D004900:Erythroblasts / Q000378:metabolism*; D004920:Erythropoiesis; D005786:Gene Expression Regulation; D008954:Models, Biological; D009154:Mutation; D009857:Oncogenes; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D013696:Temperature
D011505:Protein-Tyrosine Kinases