Action of the nonsteroidal anti-inflammatory agent, flufenamic acid, on calcium movements in isolated mitochondria.
The anti-inflammatory agent flufenamic acid was found to inhibit calcium uptake in isolated mitochondria at low concentrations (IC50 = 7.2 microM). Similar concentrations were required to promote the release of calcium from mitochondria preloaded with the cation (EC50 = 3.5 microM). Identical actions were found with diflunisal, mefenanamic acid and 2,4-dinitrophenol. It was concluded that flufenamic acid was affecting calcium movements across the mitochondrial membrane by virtue of its ability to uncouple oxidative phosphorylation.
McDougall
P
P
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Sunderland Polytechnic, U.K.
Markham
A
A
Cameron
I
I
Sweetman
A J
AJ
D019297:2,4-Dinitrophenol; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005439:Flufenamic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats
D004140:Dinitrophenols; D005439:Flufenamic Acid; D019297:2,4-Dinitrophenol; D002118:Calcium