Selectively enhanced procollagen gene expression in sclerosing (morphea-like) basal cell carcinoma as reflected by elevated pro alpha 1(I) and pro alpha 1(III) procollagen messenger RNA steady-state levels.
Sclerosing or morphea-like variant of basal cell carcinoma (BCC) is characterized by an extensive connective tissue stroma, and histopathology has suggested that the extracellular matrix is largely composed of collagen. In addition, fibronectin deposition has been proposed to modulate tumor growth in BCC. In this study, we examined the expression of genes coding for type I, III, and IV procollagens, as well as for fibronectin, in tissue from 10 patients with sclerosing BCC. For comparison, tissues from 5 patients with nodular BCC and 4 controls were examined. Total RNA was isolated by CsCl density gradient centrifugation, and messenger RNA (mRNA) steady-state levels were determined by slot-blot hybridizations with human sequence specific complementary DNAs (cDNAs). The abundance of type I procollagen mRNA in sclerosing BCC tissue was increased to 233.6 +/- 36.7% of the controls (mean +/- SEM). The corresponding value for type III procollagen mRNA in sclerosing BCC was 281.8 +/- 54.8% of the controls. Consequently, the steady-state ratio of type I/III procollagen mRNAs in sclerosing BCCs (5.0 +/- 1.2; mean +/- SD) was within the control range. Thus, there is a coordinate increase in type I and type III procollagen mRNA levels in sclerosing BCC. In contrast, the values for type I and type III procollagen mRNAs in nodular BCC were not different from the controls. In addition, type IV procollagen and fibronectin mRNA levels were not different from the controls either in sclerosing or nodular BCCs, attesting to the selectivity of the increase in type I and III procollagen mRNA levels in sclerosing BCC. These observations may relate to the excessive deposition of the extracellular matrix stroma surrounding the tumor cells in sclerosing BCC.
Moy
R L
RL
Department of Dermatology, University of Pittsburgh, Pennsylvania.
Moy
L S
LS
Matsuoka
L Y
LY
Bennett
R G
RG
Uitto
J
J
D002280:Basal Cell Carcinoma / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure; D003094:Collagen / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics; D005786:Gene Expression Regulation; D006706:Homeostasis; D006801:Humans; D008854:Microscopy, Electron; D011347:Procollagen / Q000145:classification / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D012016:Reference Values; D012594:Scleroderma, Localized / Q000473:pathology*; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure
D005353:Fibronectins; D011347:Procollagen; D012333:RNA, Messenger; D003094:Collagen