Lateral motion of fluorescent molecules embedded into cell membranes of clonal myogenic cells, L6, changes upon cell maturation.
The lateral motion of fluorescent molecules embedded into cell membranes of myogenic cell line, L6, was measured. The motion of S-F-ConA became faster at cell fusion stage, and became slower after fusion. On the other hand, the motion of lipid analog, F18, was not changed at cell fusion stage. However, after fusion when myotubes were formed, the motion of F18 became slower. At cell fusion stage, there was a large variation in the motion of S-F-ConA. This means that at this stage the properties of myoblasts change drastically and rapidly.
Kawasaki
Y
Y
Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Wakayama
N
N
Seto-Ohshima
A
A
D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D003208:Concanavalin A; D005456:Fluorescent Dyes / Q000378:metabolism*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism*; D009132:Muscles / Q000166:cytology* / Q000378:metabolism
D005456:Fluorescent Dyes; D008563:Membrane Lipids; D003208:Concanavalin A