The elastase: anti-elastase profile in lung lavage from sarcoidosis patients.
One feature of pulmonary sarcoidosis is an increase of lymphocyte numbers in bronchoalveolar lavage fluid (BALF). In some patients the number of polymorphonuclear neutrophils (PMN) also rises. It has been suggested that the prognosis for the latter subjects is likely to be worse than that for the former, but the damaging agent (or agents) released by the PMN have not yet been identified. Therefore, in the current study, the activity of one neutrophilic enzyme, elastase, which is known to damage the pulmonary interstitium, has been assessed in sarcoid BALF and compared to BALF from subjects matched for age, sex and smoking status. As BALF also contains locally-produced and serum-derived inhibitors of elastase, levels of which may change in subjects with sarcoidosis, the serine protease inhibitory capacity and the levels of three anti-elastases have been measured in the samples. Levels of the serum-derived antiproteases, alpha 1 proteinase inhibitor (alpha 1PI) and alpha 2 macroglobulin (alpha 2M) were found to be significantly increased. However, alpha 1PI/albumin and alpha 2M/albumin ratios were unchanged, suggesting that the increased levels were due to an increased permeability of the alveolar-capillary barrier. The total protease inhibitory capacity was elevated and this could be entirely explained by the raised levels of alpha 1PI and alpha 2M. Levels of the locally-produced inhibitor were unaltered. The elastolytic capacity of sarcoid BALF was unchanged. Thus, the elastase: anti-elastase balance was shifted against elastolytic activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Smith
S F
SF
Department of Medicine, Charing Cross and Westminster Medical School, London, U.K.
Guz
A
A
Burton
G H
GH
Heaton
R
R
Cooke
N T
NT
Tetley
T D
TD
D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000032:analysis* / Q000037:antagonists & inhibitors; D011480:Protease Inhibitors / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D012507:Sarcoidosis / Q000201:enzymology* / Q000473:pathology; D012907:Smoking
D011480:Protease Inhibitors; D011506:Proteins; D010196:Pancreatic Elastase