Kinetic properties from bass liver 6-phosphogluconolactonase.
By specific enzymic synthesis of the substrate 6-phosphogluconolactone "in situ", the Km for bass liver 6-phosphogluconolactonase was found to be 90 microM. This value is compatible with the kinetic parameters of both glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from bass liver, and hence with the flux through the oxidative phase of the pentose phosphate pathway.
Medina-Puerta
M M
MM
Departamento de Bioquímica de la Facultad de Veterinaria de la Universidad Complutense, Spain.
Gallego-Iniesta
M
M
Garrido-Pertierra
A
A
D000818:Animals; D001492:Bass; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism
D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D002265:Carboxylic Ester Hydrolases; C022363:6-phosphogluconolactonase