A myeloid-related sequence that localizes to human chromosome 8q21.1-22.
A myeloid-related sequence (mrs) has previously been identified that is highly expressed in selected subpopulations of myeloid leukocytes. Nucleotide sequence analysis indicates that mrs encodes what is apparently a unique 93-amino acid protein that includes an 18-amino acid leader sequence. Hybridization of an mrs cDNA probe to a Southern blot made from somatic cell hybrid DNAs shows 100% concordance with human chromosome 8, thus indicating that mrs localizes to this chromosome. In situ hybridization to metaphase chromosomes further sublocalizes mrs to bands 8q21.1-23 as 58% of the grains displayed on chromosome 8 were clustered in this region. This area encompasses the translocation breakpoint 8q22, which is rearranged in an estimated 18% of patients diagnosed with the M2 subclassification of acute nonlymphocytic leukemia (M2-ANLL). When Southern blot hybridization was performed by using somatic cell hybrid DNAs harboring either a single 8q- or a single 21q+ chromosome from two different patients with M2-ANLL, a signal was only detected in the hybrid containing the 8q-chromosome.
Mars
W M
WM
Department of Biochemistry and Molecular Biology, University of Texas, M.D. Anderson Hospital and Tumor Institute, Houston 77030.
van Tuinen
P
P
Drabkin
H A
HA
White
J W
JW
Saunders
G F
GF
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D004247:DNA / Q000235:genetics; D005796:Genes; D006801:Humans; D007962:Leukocytes / Q000502:physiology*; D014178:Translocation, Genetic
D004247:DNA