The substrate determines the rate and pattern of neutral lipid synthesized by isolated human sebaceous glands.
Lipogenesis from different substrates was determined in isolated human sebaceous glands after 17-20 h in culture. Rates of total lipogenesis were 1003 +/- 141, 842 +/- 90, 481 +/- 57 pmol.h-1 gland-1 +/- SE from acetate, lactate and glucose, respectively, when present as sole substrates: the rate from glucose was significantly lower (P less than 0.01). Squalene synthesis was greatest from acetate at 479 +/- 44 pmol.h-1.gland-1; significantly higher than from lactate (281 +/- 45 pmol.h-1.gland-1) or glucose at 119 +/- 18 pmol.h-1.gland-1. Wax ester plus cholesterol ester synthesis showed similar dependence on substrate but triglyceride synthesis was unaffected. We conclude that the added substrate determines both the rate and pattern of non-polar lipid synthesized by isolated human sebaceous glands.
Middleton
B
B
Department of Biochemistry, University Medical School, Nottingham, England.
Birdi
I
I
Heffron
M
M
Marsden
J R
JR
D000085:Acetates / Q000378:metabolism; D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007532:Isoleucine / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D009924:Organ Culture Techniques; D012627:Sebaceous Glands / Q000378:metabolism*; D012867:Skin / Q000378:metabolism
D000085:Acetates; D002250:Carbon Radioisotopes; D007773:Lactates; D008055:Lipids; D007532:Isoleucine; D005947:Glucose