Characterization of 26K globular domain of a new basement membrane collagen.
In continuing our earlier studies (Biochem. Biophys. Res. Comm. 130, 1-8, 1985) on a new collagenous component (Type XIII) from lens capsule basement membrane, we report here the isolation and characterization of a 26K protein from the 4.5 M guanidine-HCl extracts of lens capsules. The 26K protein was purified by molecular sieve and DEAE-cellulose chromatography. The protein has been characterized by amino acid composition, NaDodSO4-polyacrylamide gel electrophoresis and by immunochemical analyses. On rotary shadowing, the 26K protein appears as a globule. The available information suggests its location at the terminal end of the collagenous component. The presented data show clearly that the 26K protein is a distinct new protein, different from the earlier reported NC-1 domain of type IV collagen.
Dixit
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Department of Oral Biology, Northwestern University, Chicago, Illinois.
Harrison
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Dixit
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003094:Collagen / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007903:Lens Capsule, Crystalline / Q000032:analysis*; D007908:Lens, Crystalline / Q000032:analysis*; D046911:Macromolecular Substances; D008970:Molecular Weight
D000596:Amino Acids; D046911:Macromolecular Substances; D003094:Collagen