Adrenergic and non-adrenergic neurons in the C1 and C3 areas project to locus coeruleus: a fluorescent double labeling study.
Following iontophoretic injections of the retrograde tracer Fluoro-gold into the rat locus coeruleus (LC), retrogradely labeled neurons were seen predominantly in the area of C1 adrenergic neurons in the ventrolateral medulla (nucleus paragigantocellularis; PGi) and in the area of C3 adrenergic neurons in the dorsomedial medulla (nucleus prepositus hypoglossi; PrH). Subsequent immunofluorescence for phenylethanolamine-N-methyltransferase indicated that adrenergic and non-adrenergic LC projecting neurons in both areas are interdigitated, and that 21% of LC afferent neurons in the PGi are adrenergic while only 4% of LC afferent neurons in the area of PrH are adrenergic.
Pieribone
V A
VA
Department of Biology, New York University, NY 10003.
Aston-Jones
G
G
Bohn
M C
MC
D000320:Adrenergic Fibers / Q000032:analysis / Q000166:cytology*; D000818:Animals; D005456:Fluorescent Dyes; D008125:Locus Coeruleus / Q000033:anatomy & histology* / Q000166:cytology; D008297:Male; D008526:Medulla Oblongata / Q000032:analysis / Q000033:anatomy & histology* / Q000166:cytology; D010625:Phenylethanolamine N-Methyltransferase / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012235:Rhodamines; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D012235:Rhodamines; D013266:Stilbamidines; C027755:rhodamine isothiocyanate; D010625:Phenylethanolamine N-Methyltransferase