Isolation and characterisation of discoid granules from the tegument of adult Schistosoma mansoni.
Discoid granules were isolated from the tegument of adult Schistosoma mansoni by differential centrifugation of freeze-thaw extracts. Comparison of proteins and glycoproteins from discoid granules, surface membranes and the soluble fraction implied that discoid granules and the surface membrane were functionally linked. The results are discussed in terms of discoid granule function and surface membrane organisation.
MacGregor
A N
AN
Department of Biology, University of York, Heslington, UK.
Kusel
J R
JR
Wilson
R A
RA
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005615:Freezing; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000032:analysis / Q000302:isolation & purification*; D008854:Microscopy, Electron; D012550:Schistosoma mansoni / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure
D002241:Carbohydrates; D006023:Glycoproteins; D008565:Membrane Proteins; D000469:Alkaline Phosphatase