One-step, high-yield purification of human prostatic acid phosphatase from seminal fluid by gel-filtration HPLC under nondenaturing conditions.
This is a fast, efficient method for purification to homogeneity of human prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2], from seminal fluid. Use of a "high-pressure" liquid-chromatographic gel-filtration column permits high-yield recovery of the purified enzyme with most of its enzymatic and immunological activity retained.
Rusciano
D
D
Cellular Biology Laboratory, Sclavo Research Center, Siena, Italy.
Agostini
E
E
Ceccarini
C
C
Terrana
B
B
D000135:Acid Phosphatase / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D011467:Prostate / Q000201:enzymology*; D012661:Semen / Q000201:enzymology*
D000135:Acid Phosphatase