Cooperation between nerve growth factor and laminin or fibronectin in promoting sensory neuron survival and neurite outgrowth.
Chick embryo dorsal root ganglion (DRG) neurons were purified by differential adhesion to plastic. The purified neurons were used to study the cooperation between nerve growth factor (NGF) and laminin or fibronectin in promoting neuron survival and neurite outgrowth. NGF alone supported the survival of only 20% embryonic day 10 (E10) cells, of which only 40-50% had neurites. Treatment of the substrate with fibronectin or laminin increased survival in the presence of NGF up to 80% of the seeded neurons, all of which showed extensive neurite outgrowth. Survival and neurite outgrowth were also enhanced by the combined effects of elevated potassium and laminin. In contrast to E8-10 cells, 85% of E16 neurons survived in the basal culture conditions, i.e. without additional NGF, fibronectin or laminin, although neurite outgrowth was enhanced by all 3 proteins. Antisera to NGF, laminin and fibronectin, each independently decreased survival and neurite outgrowth of DRG neurons, totally with E9 and partially with E16 cells. The results suggest that the cooperative actions of extracellular matrix proteins and NGF are essential for survival and neurite outgrowth of embryonic DRG neurons and that these neuronal requirements change during development.
Millaruelo
A I
AI
Department of Psychobiology, University of California, Irvine 92717.
Nieto-Sampedro
M
M
Cotman
C W
CW
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D003712:Dendrites / Q000187:drug effects / Q000502:physiology*; D005353:Fibronectins / Q000276:immunology / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D007106:Immune Sera; D007797:Laminin / Q000276:immunology / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000276:immunology / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D011189:Potassium Chloride / Q000276:immunology / Q000494:pharmacology; D012965:Sodium Chloride / Q000276:immunology / Q000494:pharmacology
D005353:Fibronectins; D007106:Immune Sera; D007797:Laminin; D009414:Nerve Growth Factors; D012965:Sodium Chloride; D011189:Potassium Chloride