Improved sodium hydroxide digestion method without homogenization for extraction of gentamicin from renal tissue.
Recovery of gentamicin from renal cortical tissue (assayed by fluorescence polarization immunoassay) was highest (P less than 0.05) when using a sodium hydroxide (NaOH) digestion procedure without homogenization (90.0 +/- 5.4%), followed by homogenization with NaOH digestion (85.8 +/- 7.7%) and homogenization with trichloroacetic acid precipitation (84.4 +/- 3.3%). Simple homogenization recovered the least gentamicin (59.0 +/- 5.2%; P less than 0.05).
Brown
S A
SA
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A & M University, College Station 77843.
Sugimoto
K
K
Smith
G G
GG
Garry
F B
FB
D000818:Animals; D002417:Cattle; D005454:Fluorescence Polarization; D005839:Gentamicins / Q000302:isolation & purification*; D007668:Kidney / Q000032:analysis*; D012756:Sheep; D012972:Sodium Hydroxide
D005839:Gentamicins; D012972:Sodium Hydroxide