[Obtaining immune sera to high-titre thyroxine for immunoassay].
A method of synthesis of L-thyroxine-protein conjugates was described. It included an additional step of acylation of thyroxine by succinic anhydride. The acylated derivative was activated by carbodiimide to produce a compound which could react with the protein amino-groups. Protein conjugates with a high thyroxine content (over 20 thyroxine residues per molecule) were synthesized with minimal protein cross-linking using this method. The excess of acylated thyroxine was easily dialized against alkaline buffer. The rabbits immunized monthly with such conjugates gave antisera with high titers (1:2000-1:4000 by the ELISA method) in 9-13 weeks after the first injection. The method can be applied to other thyroid hormones and some-molecular-weight antigens.
Pankovskiĭ
D B
DB
Stafeeva
O A
OA
Savitskiĭ
A P
AP
D000215:Acylation; D000818:Animals; D000937:Antigen-Antibody Reactions; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology; D013387:Succinic Anhydrides / Q000494:pharmacology; D013974:Thyroxine / Q000276:immunology*
D007106:Immune Sera; D012709:Serum Albumin; D013387:Succinic Anhydrides; C031801:succinic anhydride; D013974:Thyroxine
Poluchenie antisyvorotok k tiroksinu s vysokimi titrami dlia tseleĭ immunologicheskogo analiza.