Synthetic oligodeoxyribonucleotides showing abnormal mobilities on polyacrylamide gel electrophoresis.
We found that synthetic DNA fragments containing a GCGAAAGC sequence showed higher mobilities than oligonucleotides without the sequence on denaturing polyacrylamide gel electrophoresis. For example, the fragment, GCGAAAGCT (9mer), showed higher mobility than the corresponding 8mer (CGAAAGCT). In addition, on Maxam-Gilbert sequencing, a 21mer containing the GCGAAAGC sequence showed an abnormal pattern, which were similar to those due to compression observed on sequencing of DNAs with high GC contents, as recently reported. It was suggested that this compression was due to the increased mobilities of the specific fragments with the GCGAAAGC sequence and that these fragments took on abnormal conformations.
Hirao
I
I
Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
Naraoka
T
T
Kanamori
S
S
Nakamura
M
M
Miura
K
K
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000138:chemical synthesis
D009838:Oligodeoxyribonucleotides