Intermediates in plasmid pT181 DNA replication.
Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali denaturation. A model for pT181 replication is proposed to accommodate this apparent discrepancy.
Majumder
S
S
Department of Plasmid Biology, Public Health Research Institute, New York 10016.
Novick
R P
RP
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000302:isolation & purification* / Q000378:metabolism; D010957:Plasmids / Q000187:drug effects; D012093:Replicon / Q000187:drug effects; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C043650:RepC protein, Staphylococcus aureus