[Multiple forms of cytochrome P-450. Characteristics of isolated aminopyrine-N-demethylase (cytochrome P-450AP)].
A previously unidentified cytochrome P-450AP possessing the highest aminopyrine-N-demethylase activity has been isolated from liver microsomes of 4-isopropylaminoantipyrine-induced rats, using affinity chromatography in combination with ion-exchange chromatography with subsequent separation on hydroxyl apatite. Using radioisotope techniques, it was found that 4-isopropylaminoantipyrine induces cytochrome P-450AP synthesis de novo. The isolated cytochrome P-450AP has the following characteristics: Mr = 49,000 Da. CO-peak maximum at 450.5 mm, rate of aminopyrine demethylation in a reconstituted system-20 nmol HCHO/min/nmol of cytochrome P-450, benzphetamine-15. The hemoprotein synthesis is paralleled with the synthesis of a protein with Mr of 51,000 Da. Immunochemical analysis permitted to identify the latter protein as cytochrome P-450b. It was demonstrated that cytochrome P-450AP does not interact with the antibodies to the major phenobarbital-induced form, i.e., with cytochrome P-450b.
Gerasimov
K E
KE
Guliaeva
L F
LF
Mishin
V M
VM
Tsyrlov
I B
IB
D000633:Aminopyrine N-Demethylase / Q000096:biosynthesis / Q000302:isolation & purification*; D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000302:isolation & purification*; D004790:Enzyme Induction; D007527:Isoenzymes / Q000096:biosynthesis / Q000302:isolation & purification*; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; D000633:Aminopyrine N-Demethylase; D010634:Phenobarbital
Mnozhestvennye formy tsitokhroma P-450. Kharakteristiki izolirovannoĭ aminopirin-N-demetilazy (tsitokhroma P-450AP).