Speciation studies of serum aluminum/citrate complex by liquid chromatography-furnace AAS.
We examined, in chronic-haemodialysis patients, the in vitro effect of citrate on aluminum binding to plasma proteins, desferrioxamine, and other constituents, by gel filtration chromatography. The elution profile of these complexes was studied by equilibrium and fast-protein liquid chromatography. The aluminum distribution was determined by graphite furnace atomic absorption spectrometry. We found that citrate mobilized aluminum from plasma binding sites to form an aluminum-citrate complex, possibly of colloidal nature.
Leung
F Y
FY
Department of Clinical Biochemistry, University Hospital, University of Western Ontario, London, Canada.
Niblock
A E
AE
Bradley
C
C
Henderson
A R
AR
D000535:Aluminum / Q000378:metabolism*; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D002951:Citrates / Q000097:blood*; D019343:Citric Acid; D003676:Deferoxamine / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014511:Uremia / Q000097:blood
D002951:Citrates; D019343:Citric Acid; D000535:Aluminum; D003676:Deferoxamine