Variations in excision repair of UVB-induced pyrimidine dimers in DNA of human skin in situ.
The excision repair kinetics of UVB (280-320 nm)*-induced pyrimidine dimers in DNA of human skin in situ was determined for seventeen volunteers using a dimer-specific endonuclease from Micrococcus luteus in conjunction with agarose gel electrophoresis. Removal of pyrimidine dimers from human skin could be detected within 6 h after irradiation and the average half-life for removal of pyrimidine dimers was 11.0 h (+/- 4.3 h). However, there was significant inter-individual variability of repair as indicated by a half-life coefficient of variation of 38%.
Freeman
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Division of Biomedical Research, Lovelace Medical Foundation, Albuquerque, New Mexico 87108.
D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000378:metabolism; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008837:Micrococcus / Q000201:enzymology; D008875:Middle Aged; D011740:Pyrimidine Dimers / Q000378:metabolism*; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D011740:Pyrimidine Dimers; D004247:DNA; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer)