Establishment of hamster blastocyst-derived embryonic stem (ES) cells.
The establishment of four ES cell lines from the Syrian "golden" hamster (Mesocricetus auratus) is described. The cells can be maintained in the undifferentiated state when grown on primary mouse embryonic fibroblast feeder layers. In suspension culture they spontaneously differentiate into embryoid bodies of increasing complexity which contain a variety of tissues including embryonic ectoderm and myocardium. All four lines--one female and three male--are karyotypically normal with 44 chromosomes. Hamster is the second species from which ES cells have been established. As in mouse, the cells should be useful for developmental and transgenic studies.
Doetschman
T
T
Medical Genetics Department, University of Wisconsin, Madison 53706.
Williams
P
P
Maeda
N
N
D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D006224:Cricetinae; D004475:Ectoderm / Q000166:cytology; D004707:Endoderm / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D007621:Karyotyping; D008297:Male; D008647:Mesocricetus; D051379:Mice; D013234:Stem Cells / Q000166:cytology*