Characterization of recombinant DNA derived-human luteinizing hormone in vitro and in vivo. Efficacy in ovulation induction and corpus luteum support.
The present data are the first, to our knowledge, to demonstrate the production feasibility of a commercially available medication of pure human luteinizing hormone from recombinant DNA technology (rechLH). The rechLH preparation achieved ovulation induction and corpus luteum support in the primate menstrual cycle. The observations described herein indicate the opportunity for significant improvement in the treatment of infertile women and men who require gonadal stimulation. Recombinant DNA-derived gonadotropin products, rechLH in this case, will have several therapeutic advantages compared with current medications extracted from urine. These advantages include (1) better reliability of an available supply of hormone and (2) improved treatment flexibility in determining the optimal dose ratio of follicle-stimulating hormone and luteinizing hormone or avoidance of the long-acting effects of human chorionic gonadotropin, as the needs of individual patients may dictate.
Simon
J A
JA
Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23510.
Danforth
D R
DR
Hutchison
J S
JS
Hodgen
G D
GD
D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects*; D005260:Female; D066298:In Vitro Techniques; D007246:Infertility / Q000628:therapy; D007986:Luteinizing Hormone / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008297:Male; D008597:Menstrual Cycle / Q000187:drug effects; D051379:Mice; D010062:Ovulation Induction / Q000379:methods*; D011374:Progesterone / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D013739:Testosterone / Q000096:biosynthesis
D011994:Recombinant Proteins; D013739:Testosterone; D011374:Progesterone; D007986:Luteinizing Hormone