Cleanup of biological extracts by a Pasteur pipette column and a comparison of in vivo-accumulated chlorinated pesticide residues with other clean up systems.
Biological samples are extracted with n-hexane/acetone (60:40) and 1 mL of the concentrated extract is eluted through a pasteur pipette column prepacked with alumina (0.3 g) and silicic acid (0.25 g) with 10 mL n-hexane (containing 4% acetone). The fat and other co-extractives are retained by the column and clear eluate is directly injected on a GLC column for determination on electron capture detector. A comparison of the Pasteur pipette cleanup with the modified method of Cole et al. (1967) on 41 samples of fish, One Step Method, (Ahmad and Marolt (1986] on 86 samples of fish and Maunder et al. (1964) on 10 type of wildlife (100 samples) was made. The Pasteur pipette method gives results which are significantly higher (p greater than 0.5) than the other methods except the One step method. The Pasteur pipette method has a detection limit of 0.01 microgram/g for DDT and its metabolites.
Ahmad
N
N
Chemistry Branch, Biological and Chemical Research Institute, NSW, Australia.
Marolt
R S
RS
Singh
G
G
D000096:Acetone; D000818:Animals; D001001:Anura / Q000378:metabolism; D001717:Birds / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D003634:DDT / Q000032:analysis; D004531:Eggs / Q000032:analysis; D005395:Fish Oils / Q000032:analysis; D005399:Fishes / Q000378:metabolism; D006586:Hexanes; D007306:Insecticides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*
D005395:Fish Oils; D006586:Hexanes; D007306:Insecticides; D010573:Pesticide Residues; D000096:Acetone; C026385:n-hexane; D003634:DDT