Analysis of amino acids in brain and plasma samples by sensitive gas chromatography-mass spectrometry.
Gas chromatography-mass spectrometry-selected-ion monitoring provided a simple and sensitive method for analyzing amino acids in plasma and brain samples. Although the sensitivities of chemical ionization and electron-impact ionization were similar chemical ionization produced higher-mass ions, which might increase the selectivity of the assay. Both chemical and electron-impact ionization distinguished the natural amino acids from the 15N-labelled amino acids. The recovery of amino acids from plasma and brain samples was ca. 75%. The amino acid levels determined by gas chromatography-mass spectrometry were comparable with the amino acid levels determined by high-performance liquid chromatography or amino acid analyzer.
Singh
A K
AK
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Ashraf
M
M
D000596:Amino Acids / Q000032:analysis* / Q000097:blood; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D051381:Rats; D009764:o-Phthalaldehyde
D000596:Amino Acids; D009764:o-Phthalaldehyde