Centrifugal column chromatography for small sample purification.
We evaluated several gel filtration materials for suitability in spun column chromatography a rapid desalting method. The materials were evaluated for resistance to column cracking and to radial shrinkage for sample recovery and for salt separation. A series of graphs can be derived from these evaluations which relates the gel heights to sample volumes for maximum recovery and salt separation while maintaining low sample dilution.
Scouten
W H
WH
Department of Chemistry, Baylor University, Waco, TX 76798.
Elhardt
C E
CE
D002498:Centrifugation; D002850:Chromatography, Gel / Q000379:methods*