title: A cytopreparatory method for cerebrospinal fluid in which the cell yield is high and the fluid is saved for chemical analysis.
abstract: A method for the concentration of cells from cerebrospinal fluid is described. An adaptation of a commercial cytochamber, consisting of a holder that fixes a disposable chamber directly on a microscope slide, was used. The cells were spun down in a conventional swing-out centrifuge, which was provided with a bucket for the cytochamber system. After removing most of the supernatant with a pipette, the remaining fluid was absorbed by means of a suction device consisting of a disposable pipette tip covered with a piece of Leukopor and filled with Sephadex G10 beads. The method gives a high recovery of cells (90%), together with a good preservation of cell morphology, and leaves about 80% of the fluid available for analysis of the soluble components.
authors: Department of Clinical Chemistry, Academic Medical Center, Amsterdam, The Netherlands.
authors: J A
authors: JA
authors: Tutuarima
authors: E A
authors: EA
authors: Hische
authors: R M
authors: RM
authors: Sylva-Steenland
authors: H J
authors: HJ
authors: van der Helm
mesh_terms: D002498:Centrifugation; D002555:Cerebrospinal Fluid / Q000032:analysis / Q000166:cytology*; D006801:Humans; D007958:Leukocyte Count