Endogenous synthesis of taurine and GABA in rat ocular tissues.
The endogenous production of taurine and gamma-aminobutyric acid (GABA) in rat ocular tissues was investigated. The activities of taurine-producing enzyme, cysteine sulfinic acid decarboxylase (CSAD), and GABA-synthesizing enzyme, glutamic acid decarboxylase (GAD), were observed in the retina, lens, iris-ciliary body and cornea. The highest specific activity of CSAD was in the cornea and that of GAD in the retina. The discrepancy between CSAD activity and taurine content within the ocular tissues indicates that intra- or extraocular transport processes may regulate the concentration of taurine in the rat eye. The GAD activity and the content of GABA were distributed in parallel within the rat ocular tissues. The quantitative results suggest that the GAD/GABA system has functional significance only in the retina of the rat eye.
Heinämäki
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AA
Department of Biochemistry, University of Oulu, Finland.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005123:Eye / Q000201:enzymology / Q000378:metabolism*; D005136:Eye Proteins / Q000032:analysis; D066298:In Vitro Techniques; D051381:Rats; D011916:Rats, Inbred F344; D013654:Taurine / Q000096:biosynthesis*; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
D000596:Amino Acids; D005136:Eye Proteins; D013654:Taurine; D005680:gamma-Aminobutyric Acid