Effect of methylene blue on the absorbance of solutions of haemoglobin.
The effect of the i.v. administration of methylene blue dye on haemoglobin oxygen saturation, measured by a pulse oximeter was investigated. The absorption spectra of various concentrations of haemoglobin, oxyhaemoglobin and methylene blue were compared. The amount of 660-nm light absorbed by methylene blue was sufficient to cause a factitious haemoglobin desaturation as measured by the pulse oximeter.
Gorman
E S
ES
Department of Anesthesia, Harvard Medical School, Boston, MA.
Shnider
M R
MR
D000368:Aged; D005260:Female; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008751:Methylene Blue / Q000494:pharmacology*; D010092:Oximetry; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000032:analysis; D013053:Spectrophotometry
D006454:Hemoglobins; D010108:Oxyhemoglobins; D010100:Oxygen; D008751:Methylene Blue