Phenobarbital, dexamethasone and benzanthracene induce several cytochrome P450 mRNAs in rat hepatoma cells.
Hepatoma cells derived from the Reuber H35 rat hepatoma express cytochrome P450 enzymes of two major families: polycyclic aromatic hydrocarbon-inducible forms are found in both differentiated and dedifferentiated cells while phenobarbital (PB)-inducible forms are found only in differentiated cells. We report here that (i) benzanthracene and PB induce P450 c mRNA in differentiated and dedifferentiated cells and (ii) dexamethasone and PB induce P450 b/e and/or P450 PB1 mRNAs in differentiated cells but not in dedifferentiated cells.
Corcos
L
L
Département de Biologie Moléculaire, Institut Pasteur, Paris, France.
Weiss
M C
MC
D000818:Animals; D001551:Benz(a)Anthracenes / Q000494:pharmacology*; D002454:Cell Differentiation; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008297:Male; D009693:Nucleic Acid Hybridization; D010634:Phenobarbital / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011912:Rats, Inbred ACI
D001551:Benz(a)Anthracenes; D012333:RNA, Messenger; D003907:Dexamethasone; D003577:Cytochrome P-450 Enzyme System; C030935:benz(a)anthracene; D010634:Phenobarbital