title: Liposomes as model for taste cells: receptor sites for bitter substances including N-C=S substances and mechanism of membrane potential changes.
abstract: Various bitter substances were found to depolarize liposomes. The results obtained are as follows: (1) Changes in the membrane potential of azolectin liposomes in response to various bitter substances were monitored by measuring changes in the fluorescence intensity of 3,3'-dipropylthiocarbocyanine iodide [diS-C3(5)]. All the bitter substances examined increased the fluorescence intensity of the liposome-dye suspension, which indicates that the substances depolarize the liposomes. There existed a good correlation between the minimum concentrations of the bitter substances to depolarize the liposomes and the taste thresholds in humans. (2) The effects of changed lipid composition of liposomes on the responses to various bitter substances vary greatly among bitter substances, suggesting that the receptor sites for bitter substances are multiple. The responses to N-C=S substances and sucrose octaacetate especially greatly depended on the lipid composition; these compounds depolarized only liposomes having certain lipid composition, while no or hyperpolarizing responses to these compounds were observed in other liposomes examined. This suggested that the difference in "taster" and "nontaster" for these substances can be explained in terms of difference in the lipid composition of taste receptor membranes. (3) It was confirmed that the membrane potential of the planar lipid bilayer is changed in response to bitter substances. The membrane potential changes in the planar lipid bilayer as well as in liposomes in response to the bitter substances occurred under the condition that there is no ion gradient across the membranes. These results suggested that the membrane potential changes in response to bitter substances stem from the phase boundary potential changes induced by adsorption of the substances on the hydrophobic region of the membranes.
authors: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors: T
authors: T
authors: Kumazawa
authors: T
authors: T
authors: Nomura
authors: K
authors: K
authors: Kurihara
mesh_terms: D052160:Benzothiazoles; D002232:Carbocyanines; D055598:Chemical Phenomena; D002621:Chemistry; D005456:Fluorescent Dyes; D008081:Liposomes; D008564:Membrane Potentials; D008954:Models, Biological; D011804:Quinolines; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013649:Taste; D013650:Taste Buds / Q000502:physiology*
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D008081:Liposomes; D011804:Quinolines; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine