Activation of a well-behaved cell cycle in araC-treated V79 cells by caffeine.
.3-1.0 microM araC (cytosine arabinoside) treatment of V79 cells produced inhibition of multiplication of cells which was accompanied by a large increase of cell size. In presence of 1-2 mM caffeine the inhibition of cell proliferation due to araC treatment was substantially reduced and cell-size increase was prevented; caffeine did not influence the uptake of araC by V79 cells. Flow microfluorometric analysis showed that caffeine induced a wave of cell cycle progression in 0.3 microM araC-treated cells. The cell cycle activated by caffeine in 0.3 microM araC-treated cells was largely well behaved; this was indicated by the fact that (1) prior to cell division cells achieved a tetraploid DNA content and (2) following cell division they had diploid DNA content as a result of which DNA homeostasis was maintained. At 1.0 microM araC concentration, however, extreme micronucleation was observed which gave rise to a substantial fraction of micronuclei with less than G1 DNA content.
Das
S K
SK
Department of Pathology, University of Washington, Seattle 98195.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D003561:Cytarabine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects; D008168:Lung; D008297:Male
D003561:Cytarabine; D002110:Caffeine; D004247:DNA