[Tyrosyl-tRNA synthetase from the bovine liver. Isolation and physico-chemical properties].
Tyrosyl-tRNA synthetase of beef liver has been isolated and its properties have been studied. Tyrosyl-tRNA synthetase is a structural dimer of alpha 2 type. Mr of the enzyme subunit is about 59 kDa. Km values for substrates have been determined and compared with kinetic properties of tyrosyl-tRNA synthetases from different sources. The polymorphism of tyrosyl-tRNA synthetase was studied. The enzyme was separated into two different forms by chromatography on phosphocellulose P 11. P1-form is active only in the amino acid activation reaction. This form is not due to the phosphorylation of the enzyme. The low molecular weight form (38 kDa) was also isolated. This form appeared due to the limited endogenic proteolysis of the main form and retained full activity in the aminoacylation reaction. Tyrosyl-tRNA synthetase from beef liver has non-specific affinity to rRNA-sepharose.
Korneliuk
A I
AI
Kurochkin
I V
IV
Matsuka
G Kh
GKh
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D014448:Tyrosine-tRNA Ligase / Q000032:analysis / Q000302:isolation & purification*
D046911:Macromolecular Substances; D000604:Amino Acyl-tRNA Synthetases; D014448:Tyrosine-tRNA Ligase
Tirozil-tRNK-sintetaza iz pecheni byka. Vydelenie i fiziko-khimicheskie svoĭstva.