Use of filter paper discs as substrate for collection and storage of blood samples for screening of anti-tetanus toxoid antibodies.
A sensitive and specific enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to tetanus in dried blood samples spotted on filter paper discs has been developed. A good correlation was found between the two methods, filter paper disc assay and direct blood assay (r = 0.91). The specificity of the test was determined by a competitive inhibition assay using goat anti-tetanus-toxoid antibodies coupled to horseradish peroxidase. The interassay coefficient of variation is 10-12%. The antibodies were measurable until 4 months of storage of filter paper discs at room temperature (25 degrees C). This procedure is useful for mass screening of anti-tetanus antibodies using dried blood samples on filter paper discs.
Ganju
L
L
National Institute of Immunology, JNU Complex, Shahid Jeet Singh Marg, New Delhi, India.
Gaur
A
A
Talwar
G P
GP
D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D001800:Blood Specimen Collection / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D013745:Tetanus Toxoid / Q000276:immunology*
D000907:Antibodies, Bacterial; D013745:Tetanus Toxoid