Effect of fluororotic acid on the sialylation of the chicken liver, hepatoma MC-29 and serum glycoconjugates.
Fluororotic acid, an orotic acid analogue which interferes with the nucleotide and RNA biosynthesis, was tested to determine its effect on: a) 14C-glucosamine and 14C-N-acetylmannosamine incorporation into acid-soluble nucleotide-sugars and into b) glycoconjugates from chicken liver and hepatoma induced by the avian leukosis strain Mc-29. In vivo metabolism studies indicated that this agent alters the nucleotide biosynthesis in both tissues investigated and causes a decrease in the sialylation rate of liver, hepatoma and serum glycolipids and glycoproteins.
Ivanov
S
S
Institute of General and Comparative Pathology, Bulgarian Academy of Sciences, Sofia.
Gavazova
E
E
Antonova
M
M
Chelibonova-Lorer
H
H
D000818:Animals; D002250:Carbon Radioisotopes; D002645:Chickens; D005944:Glucosamine / Q000378:metabolism; D006001:Glycoconjugates / Q000096:biosynthesis* / Q000097:blood; D006595:Hexosamines / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012794:Sialic Acids / Q000097:blood / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006001:Glycoconjugates; D006595:Hexosamines; D012794:Sialic Acids; D009963:Orotic Acid; C001242:5-fluoroorotic acid; D005944:Glucosamine; C002022:N-acetylmannosamine