[Dynamics of amino acid and protein metabolism of laying hens after administration of 15N-labeled wheat protein. 6. 15N incorporation into the contents and tissue of segments of the gastrointestinal tract and into the pancreas].
12 colostomized laying hens received, together with a conventional feed ration, 15N labelled wheat with a 15N excess (15N') of 14.37 atom-% over 4 days. 3 animals each were butchered after 12 h, 36 h, 60 h and 108 h after the last 15N' application and, apart from various organs, the contents and the tissue of the gastro-intestinal tract of each hen was divided into 3 fractions. TCA precipitation was carried out with the contents and the tissue of the 3 fractions. Nitrogen and its atom-% 15N' were determined in the supernatant and the precipitate. The 15N' amount in the contents of the crop and the stomachs, the small and large intestines is still considerable 12 h after the last 15N wheat feeding and still clearly detectable 108 h after it. The TCA precipitable amounts of 14N and 15N' of the contents of crop and stomach and that of the small intestine agree well; they are 75% and 50% resp. of the total N. The amount of atom-% 15N' of the contents of the small and large intestines remains the same up to 36 h after the last 15N' application and is higher at the following measuring points in the contents of the large intestine. A close correlation could be ascertained between the atom-% 15N' in the contents and tissue of the small and large intestines. The TCA soluble N quotas of both 14N and 15N' in the pancreas are higher than 50%.
Gruhn
K
K
Sektion Tierproduktion und Veterinärmedizin der Karl-Marx-Universität Leipzig.
Kirchner
E
E
D000596:Amino Acids / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D003426:Crop, Avian / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism*; D004064:Digestive System / Q000378:metabolism*; D004947:Esophagus / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D007420:Intestine, Large / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism*; D014908:Triticum
D000596:Amino Acids; D004044:Dietary Proteins
Dynamik des Aminosäuren- und Proteinstoffwechsels der Legehennen nach Applikation von 15N-markiertem. Weizenprotein. 6. Mitteilung. 15-N-Inkorporation Inhalt und Gewebe von Segmenten des Gastrointestinaltraktes und in das Pankreas.