Biochemical mapping of peptidyl-glycine alpha-amidation activity in the rat CNS.
Peptidyl-glycine alpha-amidation enzyme activity has been measured in 36 nuclei or areas in the rat CNS and pituitary using D-Tyr-Phe-Gly as the substrate. The distribution of this enzyme is highly uneven, with highest activity levels (greater than 30 pmol/mg of protein/h) in hypothalamic nuclei, substantia grisea centralis, and nucleus ruber; moderate activity levels (10-30 pmol/mg of protein/h) in globus pallidus, septum, midbrain, pons, medulla oblongata, and cervical spinal cord; and low activity levels (1-10 pmol/mg of protein/h) in other telencephalic and thalamic structures. Almost no alpha-amidation activity (less than 0.5 pmol/mg of protein/h) was detected in cerebellar cortex. The Km values in several brain regions are of the same order.
Meng
F
F
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Tsou
K
K
D000818:Animals; D001921:Brain / Q000201:enzymology*; D007031:Hypothalamus / Q000201:enzymology; D007700:Kinetics; D008297:Male; D008526:Medulla Oblongata / Q000201:enzymology; D008636:Mesencephalon / Q000201:enzymology; D006899:Mixed Function Oxygenases; D009097:Multienzyme Complexes; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D010902:Pituitary Gland / Q000201:enzymology; D011149:Pons / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000201:enzymology*; D013687:Telencephalon / Q000201:enzymology; D013788:Thalamus / Q000201:enzymology; D014018:Tissue Distribution
D009097:Multienzyme Complexes; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D000587:Oxidoreductases Acting on CH-NH Group Donors