title: The chromosomal normality of in vitro-fertilized rabbit oocytes.
abstract: The chromosomal normality of rabbit oocytes fertilized in vitro was examined. Ovum donors were superovulated with pregnant mare's serum gonadotropin and human chorionic gonadotropin (hCG). Follicular oocytes were collected laparoscopically 9-10 h after hCG treatment and incubated in vitro with spermatozoa capacitated in vivo. Of 267 aspirated oocytes, 191 (71.4%) were fertilized in vitro and developed to the 2- to 8-cell stage 24-48 h after insemination. In the chromosomal studies, 121 (63.4%) were examined. Of these, 94 (77.7%) had a normal diploid complement of chromosomes (2n = 44) and 6 (5%) showed aneuploidy. Of the remaining 21, 20 were triploid and one was tetraploid. The incidence of triploid oocytes after in vitro fertilization was higher than the rate in vivo (16.5% vs. 9.0%, respectively). These triploid oocytes were suspected to be the result of polyspermic fertilization in vitro. In addition, at the Metaphase II stage, 62 (89.9%) of 69 induced, preovulatory oocytes had a normal number of chromosomes.
authors: Department of Obstetrics and Gynecology, Asahikawa Medical School, Japan.
authors: T
authors: T
authors: Asakawa
authors: M
authors: M
authors: Ishikawa
authors: T
authors: T
authors: Shimizu
authors: W R
authors: WR
authors: Dukelow
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D004171:Diploidy; D005307:Fertilization in Vitro; D009865:Oocytes / Q000648:ultrastructure*; D010451:Peptide PHI / Q000494:pharmacology; D011123:Polyploidy; D011817:Rabbits; D012730:Sex Chromosomes; D015053:Zygote / Q000648:ultrastructure*
chemical_list: D010451:Peptide PHI