Enzymatic synthesis of delta sleep-inducing peptide.
The delta sleep-inducing peptide was assembled enzymatically from three tripeptide fragments. All the peptide bonds were prepared by either papain- or alpha-chymotrypsin-mediated synthesis. Secondary hydrolysis was suppressed by introducing N alpha-protected amino acid or peptide esters as carboxyl components and using an alkaline pH. The protected nonapeptide was oxidized with ferric chloride to deprotect the C-terminal phenylhydrazide and then hydrogenated. The homogeneous peptide was obtained by reversed phase high-performance liquid chromatography. Comparison of enzymatic and chemical preparations showed no obvious differences.
Sakina
K
K
Kyoto Research Laboratories, Toho Pharmaceutical Ind. Co. Ltd., Kyoto, Japan.
Kawazura
K
K
Morihara
K
K
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000378:metabolism*; D003701:Delta Sleep-Inducing Peptide / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D010206:Papain / Q000378:metabolism*
D007202:Indicators and Reagents; D003701:Delta Sleep-Inducing Peptide; D002918:Chymotrypsin; D010206:Papain