The search for an 111In labeled agent for the solid component of gastric emptying.
111In-labeled solid meal was prepared by chelation of 111In with Chelex resin bead. The effect of grinding of normal Chelex bead on 111In chelation and retention in solid meal was evaluated in an in vitro system. The Chelex resin beads were ground in a mortar-pestle to form ground Chelex resin beads. Fine particles were removed by resuspension in distilled water and centrifugation (1000 g). One hundred to 150 microCi of 111In chloride was diluted with 0.1 N HCl and mixed with 1 g of Chelex resin beads. Unbound 111In was removed by centrifugation (1000 g). The 111In-labeled Chelex resin beads were mixed with fresh egg and 111In-labeled solid meal was prepared by heating until solid. The meals were digested with HCl-pepsin (1.2 mg/mL of pepsin in 0.1 N HCl) for 4 h in a stirrer-bath (37 degrees C). Aliquots were collected at intervals for determination of 111In loss from 111In-labeled solid meal. These results suggest that 111In Chelex resin beads were retained in solid meals at a higher level than normal Chelex resin beads and other 111In-tracers.
Dewanjee
M K
MK
Section of Diagnostic Nuclear Medicine, Mayo Clinic and Foundation, Rochester, MN 55905.
Brown
M L
ML
Chowdhury
S
S
Thomforde
G
G
Malagelada
J
J
D004063:Digestion; D005069:Evaluation Studies as Topic; D005746:Gastric Emptying; D066298:In Vitro Techniques; D007205:Indium Radioisotopes
D007205:Indium Radioisotopes