title: Regional differences in cerebrovascular cholinergic innervation in goats.
abstract: The presence and distribution of a cerebrovascular cholinergic system were studied in goats. Regional cerebral blood flow was measured in the parietal cerebral cortex, caudate nucleus, and white matter by the hydrogen clearance technique in unanesthetized goats. Intravenous low doses of physostigmine, but not of neostigmine, significantly increased regional blood flow without changing mean arterial blood pressure or behavior. Increases of blood flow were greater in cerebral cortex and caudate nucleus than in white matter although the vasodilation induced by hypercapnia was similar in the three regions. Intracerebral microvessels were isolated from cerebral cortex, caudate nucleus, and white matter to evaluate choline acetyltransferase activity as a marker for perivascular cholinergic nerves. The enzyme level was higher in vessels from cerebral cortex and caudate nucleus than in vessels from white matter, which is in accordance with the functional data. These results suggest the presence of a cholinergic perivascular innervation system in intracerebral microvessels. Such innervation has a nonhomogeneous distribution throughout the brain and might be implicated in the local regulation of cerebral blood flow.
authors: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma, Madrid, Spain.
authors: D
authors: D
authors: Triguero
authors: A L
authors: AL
authors: López de Pablo
authors: B
authors: B
authors: Gómez
authors: C
authors: C
authors: Estrada
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000502:physiology; D002536:Cerebral Arteries / Q000378:metabolism; D002560:Cerebrovascular Circulation / Q000187:drug effects; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D005260:Female; D006041:Goats; D010830:Physostigmine / Q000494:pharmacology
chemical_list: D010830:Physostigmine; D002795:Choline O-Acetyltransferase