Transplanted astrocytes reduce synaptic density in the neuropil of cerebellar cultures.
Cytosine arabinoside-treated neonatal mouse cerebellar cultures, devoid of granule cells and mature glia, demonstrate heterologous synapses between sprouted Purkinje cell recurrent axon collaterals and dendritic spines in the neuropil. Such cultures were transplanted with optic nerve as a source of glia, and the effect on neuropil synapses was investigated. There was a significant reduction in the number of synapses in the neuropil and an increase in the number of free dendritic spines. Many of these spines occurred in clusters, unapposed by glial processes. The effect on the synapse density was not due to a comparable increase in the area occupied by the added astrocytes or an increase in nerve terminal diameter. The results suggest that astrocytes alter the density of neuropil synapses and may also induce the sprouting of dendritic spines.
Meshul
C K
CK
Neurology Research, Veterans Administration Medical Center, Portland, OR.
Seil
F J
FJ
D000818:Animals; D001253:Astrocytes / Q000502:physiology* / Q000637:transplantation / Q000648:ultrastructure; D002478:Cells, Cultured; D002531:Cerebellum / Q000502:physiology* / Q000648:ultrastructure; D003561:Cytarabine / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron; D009900:Optic Nerve / Q000637:transplantation; D013569:Synapses / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
D003561:Cytarabine