Differences in neuronal lipid composition between superior cervical ganglia and nodose ganglia of the rat.
The lipid content and composition of rat superior cervical ganglia containing sympathetic motor neurons and nodose ganglia containing parasympathetic sensory neurons were studied for the first time to elucidate the mechanism of the different effects of exogenous gangliosides on these neurons in the culture medium. The ganglioside content of the superior cervical ganglia was almost 3-times that of the nodose ganglia. Although both ganglia contained GM3, GD3, GD1b and GT1b as major gangliosides, the nodose ganglia additionally contained a significant amount of sialosyllactoneotetraosylceramide LM1 (10% of total sialic acids). Contrasting with nodose ganglia, vagus fiber and dorsal root ganglia of rats, superior cervical ganglia had a higher content of sulfatide than galactosylceramide. The phospholipid content was lower in superior cervical ganglia than in nodose ganglia. Superior cervical ganglia contained less ethanolamine plasmalogen and more phosphatidylcholine than nodose ganglia. Sphingomyelin in superior cervical ganglia contained mainly medium-chain fatty acids, while that in nodose ganglia contained mainly longer-chain fatty acids. Differences in the fatty acid composition of glycerophospholipids were also observed. The results indicate that the properties of neuronal cell membranes from superior cervical ganglia and nodose ganglia are quite different, and that the differences may reflect the physiological roles of these ganglia.
Hara
A
A
Department of Lipid Biochemistry, Shinshu University School of Medicine, Matsumoto, Japan.
Taketomi
T
T
Iwata
M
M
Ando
M
M
Nagata
Y
Y
D000818:Animals; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D003720:Densitometry; D005227:Fatty Acids / Q000032:analysis; D005727:Ganglia, Spinal / Q000032:analysis*; D005728:Ganglia, Sympathetic / Q000032:analysis*; D005732:Gangliosides / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D009474:Neurons / Q000032:analysis; D009620:Nodose Ganglion / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D014630:Vagus Nerve / Q000032:analysis*
D005227:Fatty Acids; D005732:Gangliosides; D008055:Lipids; D010743:Phospholipids