title: Amino acid sequence of bovine spleen cathepsin B.
abstract: The complete amino acid sequence of bovine spleen cathepsin B was determined by manual and automatic Edman degradation of fragments prepared by proteolytic or chemical digestion of the enzyme. The single-chain form of the enzyme consists of 253 amino acid residues and its Mr is 27,468 (carbohydrate moiety not included). The light chain (residues 1-47) and the heavy chain (residues 50-253) of the enzyme are linked by the sequence -Gly-Arg (residues 48 and 49) in the single-chain form. Bovine spleen cathepsin B shows 80% sequence homology with cathepsins B from other species. An outstanding feature of bovine spleen cathepsin B not observed with the other cathepsins B is the presence of two additional half-cystine residues.
authors: Department of Biochemistry, Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Science, Prague.
authors: B
authors: B
authors: Meloun
authors: M
authors: M
authors: Baudys
authors: J
authors: J
authors: Pohl
authors: M
authors: M
authors: Pavlík
authors: V
authors: V
authors: Kostka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002401:Cathepsin B / Q000302:isolation & purification; D002417:Cattle; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology*
chemical_list: D002401:Cathepsin B