Quantitative studies of enkephalin's coexistence with gamma-aminobutyric acid, glycine and neurotensin in amacrine cells of the chicken retina.
Previous double-label studies demonstrate that enkephalin coexists with gamma-aminobutyric acid, glycine or neurotensin in amacrine cells of the chicken retina. The present study utilizes double- and triple-label paradigms to quantitatively analyze these coexisting relationships. Twenty-eight percent of enkephalin-like immunoreactive amacrine cells were found to exhibit high-affinity uptake of [3H]GABA, while 53% of enkephalin-amacrine cells specifically accumulate [3H]glycine. Moreover, the present study predicts that at least 26% of enkephalin-amacrine cells which accumulate [3H]glycine should also be immunoreactive for neurotensin.
Watt
C B
CB
Center for Biotechnology, Baylor College of Medicine, Woodlands, TX 77381.
Li
T
T
Lam
D M
DM
Wu
S M
SM
D000818:Animals; D001345:Autoradiography; D002645:Chickens; D004745:Enkephalins / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism*; D007150:Immunohistochemistry; D009496:Neurotensin / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D004745:Enkephalins; D009496:Neurotensin; D005680:gamma-Aminobutyric Acid; D005998:Glycine