Two methods that facilitate autoradiography of small 32P-labeled DNA fragments following electrophoresis in agarose gels.
Two methods which permit detection by autoradiography of small 32P-labeled DNA fragments resolved by agarose gel electrophoresis are described. Agarose gel electrophoresis poses problems for autoradiography as (i) the gels are normally too thick to allow autoradiography without being dried first, and (ii) fragments of DNA of 1000 bp or less in length are readily lost during drying. In this study DNA fragments as small as 121 bp have been retained in agarose gels upon drying. This has been achieved by either (i) first fixing the DNA with the cationic detergent cetyltrimethylammonium bromide, or (ii) drying the agarose gels onto Zeta-Probe charge-modified membranes.
Cockerill
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PN
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
D001345:Autoradiography; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D010761:Phosphorus Radioisotopes
D002593:Cetrimonium Compounds; D010761:Phosphorus Radioisotopes; D004247:DNA; D000077286:Cetrimonium