Intestinal-type glycosphingolipids in urine from patients with enterocutaneous urinary diversions.
Ureteroenterocutaneous stoma is a surgical procedure for urinary diversion by which an intestinal segment is used as a conduit or reservoir for the urine. The intestinal mucosa continues to produce a mucous gel containing desquamated epithelial cells. This mucous gel is easily obtainable from the urine. Glycosphingolipids were isolated from such gels and analyzed with immunological methods using a thin-layer chromatography overlay assay and mass spectrometry. The glycosphingolipids obtained mainly belong to the lacto-type I series comprising ABH, Lea, and Leb determinants. These observations support the intestinal origin of the glycosphingolipids. A regional distribution of the ABH and Lewis antigens, which is in accordance with previous immunohistochemical findings, was observed.
Mårtensson
S
S
Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Hansson
G C
GC
Lundblad
A
A
D002855:Chromatography, Thin Layer; D003106:Colon / Q000601:surgery; D012809:Colon, Sigmoid / Q000601:surgery; D008401:Gas Chromatography-Mass Spectrometry; D006028:Glycosphingolipids / Q000652:urine*; D006801:Humans; D007082:Ileum / Q000601:surgery; D014547:Urinary Diversion
D006028:Glycosphingolipids