Radiation induction of micronuclei in human lymphocytes.
An examination of the cytokinesis-blocked micronucleus technique confirmed its potential usefulness as a method of biological dosimetry for radiation accidents. Several advantages and disadvantages of the system are discussed. It has been demonstrated that under the conditions of these experiments, the blocking agent, cytochalasin B does not induce micronuclei or unstable chromosome aberrations. The induction of sister-chromatid exchanges proved just significant. Analysis of the dose response for 250 kVp X-rays indicates that although the Y = alpha D + beta D2 model fits the data, the relationship does not correspond to that for total aberration induction as might have been expected. The background frequency of micronuclei and the value of the alpha coefficient are higher than for total aberrations and the beta term is lower. This indicates that simple incorporation of acentric chromosome fragments into micronuclei may not wholly account for the phenomenon.
Prosser
J S
JS
National Radiological Protection Board, Didcot, Oxon, Great Britain.
Moquet
J E
JE
Lloyd
D C
DC
Edwards
A A
AA
D000328:Adult; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003571:Cytochalasin B / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D012854:Sister Chromatid Exchange / Q000528:radiation effects; D014965:X-Rays
D003571:Cytochalasin B