Enhancement of anti-phospholipid antibody activity by Tween 20.
Addition of the non-ionic detergent Tween 20 to the serum diluent enhances anti-cardiolipin binding reactivity in an ELISA system. Maximal enhancement was obtained using a concentration of 0.05% Tween 20 in the diluent. Non-specific interactions were also considerably reduced.
Ming
C H
CH
Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur.
Fan
Y S
YS
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002308:Cardiolipins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011136:Polysorbates / Q000494:pharmacology*
D000906:Antibodies; D002308:Cardiolipins; D011136:Polysorbates