A biotin-avidin sandwich enzyme-linked immunosorbent assay of growth hormone in bovine plasma.
Purified pituitary bovine growth hormone (bGH) has been used to develop a homologous sandwich enzyme-linked immunosorbent assay in which affinity-purified antibodies are immobilized on microtiter plates. Bovine GH bound to specific antibody is then revealed with a second anti-bGH antibody labeled with biotin and peroxidase-conjugated avidin. The method requires only 48 h, including the coating step, and has a sensitivity as low as 0.25 ng/ml of bGH. Statistical analyses (test of parallelism, cross-reactivity among bGH and GH of various species and bovine prolactin, recovery test, within- and between-assay variation, comparison with radioimmunoassay) confirm the high specificity and reproducibility of the method.
Secchi
C
C
Institute of Veterinary Physiology and Biochemistry, University of Milan, Italy.
Biondi
P A
PA
Berrini
A
A
Simonic
T
T
Ronchi
S
S
D000818:Animals; D001360:Avidin; D001710:Biotin; D002417:Cattle / Q000097:blood*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D013006:Growth Hormone / Q000097:blood* / Q000276:immunology; D007106:Immune Sera; D008322:Mammals / Q000276:immunology; D013045:Species Specificity
D007106:Immune Sera; D001360:Avidin; D001710:Biotin; D013006:Growth Hormone