Purification of proteins from polyacrylamide gels, free of detergent or dye.
A two step procedure recovers proteins from sodium dodecyl sulfate polyacrylamide gels. The proteins are eluted by electrophoretic dialysis. The eluent is then passed through an Amberlite CG-400 anion-exchange resin. The recovery of protein is nearly total. The recovered proteins have no detectable sodium dodecyl sulfate contamination. With gels that have been stained with Coomassie Brilliant Blue R, the procedure recovers the proteins free of the dye. We have used this procedure successfully during the purification of epidermal glycoproteins.
Brysk
M M
MM
Department of Dermatology, University of Texas Medical Branch, Galveston 77550.
Barlow
E
E
Bell
T
T
Rajaraman
S
S
Stach
R W
RW
D002852:Chromatography, Ion Exchange; D004396:Coloring Agents; D003902:Detergents; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000302:isolation & purification*
D004396:Coloring Agents; D003902:Detergents; D011506:Proteins