Factors affecting the rates at which nucleotides bind to Dowex 1 resin.
The relative rates at which adenine nucleotides bind to Dowex 1 anion-exchange resin are determined by diffusion through the immobile fluid film surrounding the resin particles and thus are unaffected by how tightly the nucleotides bind. By using a fine resin and stirring efficiently, the time needed for equilibration can be reduced from 4 h to less than 3 min.
Jenkins
W T
WT
Department of Chemistry, Indiana University Bloomington 47405.
Schweers
L A
LA
D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate; D000327:Adsorption; D000837:Anion Exchange Resins; D001665:Binding Sites; D007475:Ion Exchange Resins; D007700:Kinetics; D010316:Particle Size; D012117:Resins, Synthetic
D000227:Adenine Nucleotides; D000837:Anion Exchange Resins; D007475:Ion Exchange Resins; D012117:Resins, Synthetic; C027972:Dowex; D000255:Adenosine Triphosphate