[An improved chemico-enzymatic method of synthesizing long DNA segments].
A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.
Kobets
M L
ML
Rivkin
M I
MI
Bogachev
V S
VS
Kumarev
V P
VP
D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D011088:DNA Ligases; D004256:DNA Polymerase I; D004277:DNA, Single-Stranded / Q000032:analysis / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005813:Genes, Synthetic; D006801:Humans; D007370:Interferon Type I / Q000235:genetics; D011089:Polydeoxyribonucleotides / Q000032:analysis / Q000096:biosynthesis*; D011118:Polynucleotide Ligases
D004277:DNA, Single-Stranded; D007370:Interferon Type I; D011089:Polydeoxyribonucleotides; D004247:DNA; D004256:DNA Polymerase I; D011088:DNA Ligases; D011118:Polynucleotide Ligases
Usovershenstvovannyĭ khimiko-fermentativnyĭ metod sinteza protiazhennykh fragmentov DNK.