Quantitation of human chorionic gonadotrophin by the planimetry of latex agglutination-inhibition results on microtiter plates.
A new type of heavy latex particle (LP) coated with the conjugates of human chorionic gonadotrophin (hCG) and bovine serum albumin (BSA) was used as indicator for the microtiter agglutination-inhibition assay of hCG using a monoclonal antibody (McAb) to hCG. The precipitation patterns of LP were measured by an automatic planimetry system. The detection limit of this method was 4 to 8 IU/l. Human luteinizing hormone (hLH) and follicle-stimulating hormone (FSH) caused hardly any cross-reaction in the presence of 3,350 IU/l and 1,500 IU/l, respectively. This planimetric immunoassay (PMIA) method provides a potentially useful method for hormone assay.
Matsuzawa
S
S
Department of Forensic Medicine, School of Medicine, Juntendo University, Tokyo, Japan.
Kimura
H
H
Suzuki
H
H
D000372:Agglutination Tests; D000911:Antibodies, Monoclonal / Q000276:immunology; D006063:Chorionic Gonadotropin / Q000032:analysis* / Q000276:immunology; D003201:Computers; D004797:Enzyme-Linked Immunosorbent Assay; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin