Identification of cDNAs corresponding to mosquito ribosomal protein genes.
Sequences encoding mosquito (Aedes albopictus) ribosomal proteins L8, L14 and L31 were identified from a cDNA library made from size-selected polyadenylated mRNA. Candidate cDNAs corresponding to moderately abundant mRNAs were screened by translation of hybrid-selected transcripts in wheat-germ lysates. Translation products were extracted with acetic acid and analyzed by electrophoresis in two dimensions in the presence of unlabeled ribosomal proteins. The identity of translation products that coelectrophoresed with purified ribosomal protein standards was supported by peptide mapping. The cDNAs corresponding to L8 (pL8) and L31 (pL31) hybridized to cytoplasmic mRNAs of 1.4 and 0.9 kb, respectively. In Southern blots of genomic DNA digested with BamHI, HindIII or EcoRI, the cDNA inserts from both pL8 and pL31 gave simple hybridization patterns suggestive of a low copy number for mosquito ribosomal protein genes.
Durbin
J E
JE
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Piscataway.
Swerdel
M R
MR
Fallon
A M
AM
D000330:Aedes / Q000502:physiology*; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D005796:Genes; D010449:Peptide Mapping; D012269:Ribosomal Proteins / Q000235:genetics*
D012269:Ribosomal Proteins; D004247:DNA