title: The three-dimensional structures of S-layers of two novel Eubacterium species isolated from inflammatory human processes.
abstract: The three-dimensional structures of the crystalline surface layers of two species of Eubacteria have been determined by electron microscopy and computerized image processing. The S-layer of Eubacterium sp. ES4C has tetragonal symmetry, with a unit cell spacing of 10.6 nm and a thickness of 9.5 nm, while that of Eubacterium sp. AHN 990 has hexagonal symmetry a = b = 15.7 nm and a thickness of 13 nm. The resolutions in the reconstructions were 2.5 nm and 1.8 nm, respectively. The reconstruction of the S-layer of strain ES4C reveals a distinct domain structure: a major tetramer, arms connecting adjacent unit cells, and a minor tetramer. The S-layer of strain AHN 990, on the other hand, has a rather complex arrangement, centred around the six-fold axis.
authors: Department of Structural Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
authors: A
authors: A
authors: Sjögren
authors: D N
authors: DN
authors: Wang
authors: S
authors: S
authors: Hovmöller
authors: M
authors: M
authors: Haapasalo
authors: H
authors: H
authors: Ranta
authors: E
authors: E
authors: Kerosuo
authors: H
authors: H
authors: Jousimies-Somer
authors: K
authors: K
authors: Lounatmaa
mesh_terms: D005051:Eubacterium / Q000302:isolation & purification / Q000648:ultrastructure*; D005613:Freeze Etching; D006801:Humans; D007249:Inflammation / Q000382:microbiology; D008854:Microscopy, Electron; D008961:Models, Structural