Presence of digitalis-like factor in mammalian plasma.
We attempted to purify a digitalis-like factor from volume expanded dog plasma using an inhibitory effect on the binding of [3H]ouabain to intact human erythrocytes to monitor digitalis-like activity. A highly polar [3H] ouabain displacing compound was purified to a high degree using a combination of chromatographic procedures including reverse phase and gel filtration high performance liquid chromatography. This compound, a reversible inhibitor of [3H]ouabain binding, closely resembles ouabain in its polarity and significantly increases during saline infusion. Its molecular weight was estimated to be 343Da. Moreover, similar compound was consistently detected in other mammalian plasma.
Goto
A
A
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Yamada
K
K
Ishii
M
M
Yoshioka
M
M
Ishiguro
T
T
Eguchi
C
C
Sugimoto
T
T
D001667:Binding, Competitive; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002298:Cardenolides; D004077:Digoxin; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010042:Ouabain / Q000378:metabolism; D011955:Receptors, Drug / Q000378:metabolism; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase
D001798:Blood Proteins; D002298:Cardenolides; D011955:Receptors, Drug; D012503:Saponins; C028996:cardiac glycoside receptors; C034504:digoxin-like factors; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase