Color analysis method for studying oxygen transport in hemoglobin solutions using an image-input and -processing system.
A method for quantitative analysis of hemoglobin color to estimate the oxygen saturation was developed. The method uses an image-input and -processing system composed of a 3-tube video camera and a digital image analyzer. Using the system connected to a microscope, facilitated diffusion of oxygen in hemoglobin solutions was observed and analyzed in a position-sensitive way. The results confirmed its applicability to this study and gave information about the diffusion mechanism expressed by the empirical formula J = kY, where J is the flux of oxygen, Y is the oxygen saturation of hemoglobin, and k is a constant.
Hashimoto
M
M
Department of Physico-chemical Physiology, Osaka University Medical School, Japan.
Hata
R
R
Tyuma
I
I
Isomoto
A
A
Uozumi
M
M
D003124:Colorimetry / Q000295:instrumentation / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008838:Microcomputers; D008954:Models, Biological; D010108:Oxyhemoglobins / Q000378:metabolism*; D012996:Solutions
D010108:Oxyhemoglobins; D012996:Solutions