Ultrastructural study on the meninx of the goldfish brain.
The cranial meninges of the goldfish were studied by means of transmission electron microscopy combined with the freeze-fracturing technique. The goldfish has three cranial meninges. The outer layer consists of flattened cells, which are stratified in 3 to 7 layers and are packed densely with many interdigitations of cell processes. The constituent cells in the outer layer have copious smooth endoplasmic reticulum and are joined by gap junctions but have no desmosomes. The intermediate layer is thin, continuous, and single cell. In the replicas, both the upper and the lower surfaces of the intermediate layer cells have numerous openings of pinocytotic vesicles, but the upper surface is characterized by round gap junctions, whereas the lower surface is identified by a linear continuation of a combination of tight junctions and gap junctions and by desmosomes. The lateral surface has a hexagonal network of tight junctions and gap junctions with internally located desmosomes, which functions as a barrier to intercellular movement of lanthanum. The inner layer consists of a meshwork of reticular cells and large intercellular spaces, in which fine granular material, capillaries, and different types of blood-derived free cells can be found. Cells in the inner layer contain rough endoplasmic reticulum stacked in lamellae and have irregular processes joined by desmosomes. The goldfish meninges are compared with the meninges of mammals.
Momose
Y
Y
Department of Anatomy, University of Tsukuba, Ibaraki, Japan.
Kohno
K
K
Ito
R
R
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D003530:Cyprinidae / Q000033:anatomy & histology*; D005614:Freeze Fracturing; D006054:Goldfish / Q000033:anatomy & histology* / Q000378:metabolism; D007811:Lanthanum / Q000378:metabolism; D008578:Meninges / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010539:Permeability
D007811:Lanthanum