New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork.
A new enrichment medium for the recovery of pathogenic Yersinia enterocolitica serogroup O:3 from naturally infected meat products based on three selective agents, Irgasan, ticarcillin, and potassium chlorate (ITC), was compared with several other one- or two-step enrichments. Y. enterocolitica serogroup O:3 was recovered from 96.5% of 29 pork tongues, 24% of 50 ground pork samples, 16% of 25 masseter muscle samples, and 61% of tonsils. ITC was by far the most sensitive method for the recovery of Y. enterocolitica O:3, especially from ground meat and masseter muscles, while cold and two-step enrichments yielded better results for nonpathogenic strains. Plating of ITC enrichments onto SS-deoxycholate-calcium agar gave overall better results than plating onto cefsulodin-Irgasan-novobiocin agar for serogroup O:3.
Wauters
G
G
Microbiology Unit, University of Louvain, Brussels, Belgium.
Goossens
V
V
Janssens
M
M
Vandepitte
J
J
D000818:Animals; D005516:Food Microbiology; D008460:Meat; D009132:Muscles / Q000382:microbiology; D014066:Palatine Tonsil / Q000382:microbiology; D013552:Swine; D015008:Yersinia enterocolitica / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity