2-Nitropropane induces DNA repair synthesis in rat hepatocytes in vitro and in vivo.
The genotoxicity of 2-nitropropane (2-NP) and 1-nitropropane (1-NP) was investigated by measuring the induction of DNA repair synthesis in rat liver cells in vitro and in vivo. 2-NP strongly induced DNA repair synthesis in both cases. When applied in vivo, 2-NP was considerably more effective in hepatocytes from males than in those from females. 1-NP was not active in vitro or in vivo. 2-NP and 1-NP did not induce repair in cell lines of extrahepatic origin derived from rat, mouse, hamster and man. The results are consistent with the reported carcinogenicity of 2-NP in rat liver and suggest that the formation of hepatocarcinomas by 2-NP is due to the generation of a genotoxic metabolite from 2-NP by liver-specific metabolism.
Andrae
U
U
Institut für Toxikologie, Gesellschaft für Strahlen- und Umweltforschung, Neuherberg/München, FRG.
Homfeldt
H
H
Vogl
L
L
Lichtmannegger
J
J
Summer
K H
KH
D000473:Alkanes / Q000633:toxicity*; D000818:Animals; D001711:Biotransformation; D002460:Cell Line; D004260:DNA Repair / Q000187:drug effects*; D005260:Female; D008099:Liver / Q000187:drug effects*; D008297:Male; D009153:Mutagens; D009595:Nitroparaffins / Q000378:metabolism / Q000633:toxicity*; D011407:Propane / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains
D000473:Alkanes; D009153:Mutagens; D009595:Nitroparaffins; C035314:1-nitropropane; C016617:2-nitropropane; D011407:Propane