Interaction of tRNA transcription factors with satellite I DNA from Xenopus laevis.
A cloned repeat of Xenopus laevis satellite I DNA was tested for the ability to form stable complexes with tRNA transcription factors in vitro. In template exclusion studies, the satellite I DNA competed efficiently with a tRNA gene for binding of yeast RNA polymerase III transcription factors. DNase I footprinting further showed that transcription factor TF IIIC alone bound to satellite I DNA at both the A block and B block consensus promoter sequences immediately downstream from the transcription start point. The strength and position of these associations indicate that satellite I DNA is a potential site for association of the same DNA-binding proteins that activate tRNA gene transcription.
Engelke
D R
DR
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109.
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D004276:DNA, Satellite / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012320:RNA Polymerase III / Q000378:metabolism; D012322:RNA Precursors / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D019474:Transcription Factors, TFIII; D014158:Transcription, Genetic; D014982:Xenopus laevis / Q000235:genetics*; D000098832:Transcription Factor TFIIIC
D004276:DNA, Satellite; D012322:RNA Precursors; D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; D012343:RNA, Transfer; D012320:RNA Polymerase III