title: The distinction of bone and liver isoenzymes of alkaline phosphatase in serum using a monoclonal antibody.
abstract: Out of 31 mouse monoclonal antibodies to human liver and bone ALP, one antibody coded BAP 1/9, showing a preference for binding the bone isoenzyme, and one antibody coded LAP 1/5, showing no preference, were selected to study binding of the serum forms of ALP. Using a plate capture assay format BAP 1/9 showed a 1.8-fold preference for binding purified bone versus liver ALP in the presence of 10% ALP-free serum compared with 2.1- to 2.25-fold preference in buffered salt solution. BAP 1/9 showed a preference for binding ALP from serum samples containing predominantly bone ALP compared to those containing predominantly liver ALP. Using predefined mixtures of serum forms of bone and liver ALP a linear relationship between ALP bound to BAP 1/9 and the fraction of bone ALP was obtained.
authors: Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.
authors: R N
authors: RN
authors: Seabrook
authors: E M
authors: EM
authors: Bailyes
authors: C P
authors: CP
authors: Price
authors: K
authors: K
authors: Siddle
authors: J P
authors: JP
authors: Luzio
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D000911:Antibodies, Monoclonal; D001842:Bone and Bones / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008099:Liver / Q000201:enzymology*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D000469:Alkaline Phosphatase