Comparative study on the main membrane-surface sialoglycopeptides released from young and old human erythrocytes with trypsin.
1. The amount of sialoglycopeptide (SGP) mixture released from young (Y) erythrocytes by trypsin treatment was significantly larger than that from old (O) ones. 2. The two main membrane-surface sialoglycopeptides (Y-SGP and O-SGP) were isolated in yields of about 45% and 42% from the mixtures by a combination of Sephadex G-50 superfine gel filtration with DEAE-cellulose chromatography, respectively. Molecular weights of 12,000 and 12,100, respectively, were found for the Y-SGP and the O-SGP. 3. The Y-SGP showed a greater proportion of sialic acid than the O-SGP. The contents of galactose, N-acetylglucosamine and N-acetylgalactosamine of the Y-SGP were significantly lower than those of the O-SGP. 4. Both the SGPs exhibited the same reactivities against the MN reagents, but the reactivities against the lectins of Limulus polyphemus and Phaseolus vulgaris (E) were stronger in the Y-SGP than in the O-SGP. Neither the Y-SGP nor the O-SGP showed the reactivity to Arachis hypogaea (anti-T) lectin.
Shinozuka
T
T
Department of Legal Medicine, School of Medicine, Keio University, Japan.
Takei
S
S
Yanagida
J
J
Watanabe
H
H
Ohkuma
S
S
D000596:Amino Acids / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000032:analysis*; D006801:Humans; D012795:Sialoglycoproteins / Q000097:blood*; D014357:Trypsin / Q000378:metabolism*
D000596:Amino Acids; D012795:Sialoglycoproteins; D014357:Trypsin