Determination of aztreonam and L-arginine combination in parenteral formulations.
A rapid, sensitive and precise liquid chromatographic method is presented for the determination of aztreonam alone, in the presence of its degradation product, and in a parenteral formulation containing l-arginine. A reverse phase column and 0.2M phosphate buffer (pH 6)-methanol (95 + 5) with mobile phase at a flow rate of 2 mL/min is used. The method is sensitive for the range of 10-50 micrograms/mL with a relative standard deviation of less than 2%. The method has been applied to a parenteral formulation containing aztreonam and l-arginine. L-Arginine is also determined by a nonaqueous titrimetric method.
Wahbi
A A
AA
King Saud University, College of Pharmacy, Pharmaceutical Chemistry Department, Riyadh, Saudi Arabia.
Abounassif
M A
MA
Gad-Kariem
E R
ER
Ibrahim
M E
ME
Aboul-Enein
H Y
HY
D001120:Arginine / Q000008:administration & dosage / Q000032:analysis*; D001398:Aztreonam / Q000008:administration & dosage / Q000032:analysis*; D002853:Chromatography, Liquid; D006868:Hydrolysis; D007202:Indicators and Reagents; D007263:Infusions, Parenteral; D012997:Solvents
D007202:Indicators and Reagents; D012997:Solvents; D001120:Arginine; D001398:Aztreonam