Structural variability of the neutral carbohydrate moiety of cow colostrum kappa-casein as a function of time after parturition. Identification of a tetrasaccharide with blood group I specificity.
New neutral oligosaccharides from cow colostrum kappa-casein were identified and characterized by 500-MHz 1H-NMR spectroscopy. Their structures are Gal beta(1----3)GalNAc-ol, Gal beta(1----3)[GlcNAc beta(1----6)]GalNAc-ol, Gal beta(1----3)[Gal beta(1----4)GlcNAc beta(1----6)]GalNAc-ol, Gal beta(1----3)[Fuc alpha(1----3)[Gal beta(1----4)]GlcNAc beta(1----6)]GalNAc-ol. The tetrasaccharide and the cow colostrum kappa-caseinoglycopeptide which contains this oligosaccharide inhibit the hemagglutination of blood group I human erythrocytes. In cow mature milk only the disaccharide is characterized. The variability of these neutral oligosaccharides in cow kappa-casein as a function of time after calving is studied.
Fiat
A M
AM
Laboratoire des Protéines, Université de Paris V, France.
Chevan
J
J
Jollès
P
P
De Waard
P
P
Vliegenthart
J F
JF
Piller
F
F
Cartron
J P
JP
D000818:Animals; D001789:Blood Group Antigens; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D002364:Caseins / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003126:Colostrum / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006020:Glycopeptides / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007048:I Blood-Group System; D007518:Isoantibodies / Q000032:analysis; D007743:Labor, Obstetric; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D011247:Pregnancy; D013402:Sugar Alcohols / Q000032:analysis; D013997:Time Factors
D001789:Blood Group Antigens; D002241:Carbohydrates; D002364:Caseins; D006020:Glycopeptides; D007048:I Blood-Group System; D007518:Isoantibodies; D009844:Oligosaccharides; D013402:Sugar Alcohols; C020251:kappa-casein glycomacropeptide