Evaluation of four methods of DNA distribution data analysis based on bromodeoxyuridine/DNA bivariate data.
Four published methods of DNA-content histogram analysis (those of Fried, Dean and Jett, simplified Dean, and Fox) were compared using a double labeling of different cell populations. Partially synchronized and asynchronous cell populations were incubated with bromodeoxyuridine (BrdUrd) and then stained with an anti-BrdUrd monoclonal antibody and propidium iodide (PI). The fractions of cells in the G1, S, and G2 + M phases were calculated by each method and compared with those derived from G1, S, and G2 + M areas plotted on BrdUrd/DNA bivariate histograms, taken as the "true" values. This procedure enabled an optimal choice of method for a given cell population.
Lacombe
F
F
Laboratoire d'Hématologie, Hôpital Cardiologique Haut-Lévque, Pessac, France.
Belloc
F
F
Bernard
P
P
Boisseau
M R
MR
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008214:Lymphocytes / Q000032:analysis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011419:Propidium
D000911:Antibodies, Monoclonal; D010835:Phytohemagglutinins; D011419:Propidium; D004247:DNA; D001973:Bromodeoxyuridine