title: Hemolytic activity of a cyclic peptide Ro09-0198 isolated from Streptoverticillium.
abstract: Ro09-0198, a cyclic peptide isolated from culture filtrates of Streptoverticillium griseoverticillatum, induced lysis of erythrocytes. Preincubation of the peptide with phosphatidylethanolamine reduced the hemolytic activity, whereas other phospholipids present in erythrocytes in nature had no effect. A study of the structural requirements on phosphatidylethanolamine necessary for interaction with the peptide indicates that Ro09-0198 recognizes strictly a particular chemical structure of phosphatidylethanolamine: dialkylphosphoethanolamine as well as 1-acylglycerophosphoethanolamine showed the same inhibitory effect on hemolysis induced by Ro09-0198 as diacylphosphatidylethanolamine, whereas phosphoethanolamine gave no inhibitory effect. Neither phosphatidyl-N-monomethylethanolamine nor alkylphosphopropanolamine had an inhibitory effect. Consequently, the hydrophobic chain is necessary for the interaction and the phosphoethanolamine moiety is exactly recognized by the peptide. Ro-09-0198-induced hemolysis was temperature-dependent and the sensitivity of hemolysis differed greatly among animal species.
authors: Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors: S Y
authors: SY
authors: Choung
authors: T
authors: T
authors: Kobayashi
authors: J
authors: J
authors: Inoue
authors: K
authors: K
authors: Takemoto
authors: H
authors: H
authors: Ishitsuka
authors: K
authors: K
authors: Inoue
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents; D002855:Chromatography, Thin Layer; D003958:Diamide / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism; D010455:Peptides; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D010714:Phosphatidylethanolamines / Q000378:metabolism / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D006460:Hemolysin Proteins; D008081:Liposomes; D010455:Peptides; D010456:Peptides, Cyclic; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D003958:Diamide; C055996:lanthiopeptin; D014357:Trypsin