Fractionation of type V collagen from carcinomatous and dysplasic breast in the presence of alkaline potassium chloride.
A simple, one-step fractionation technique suitable for the finest quantitation of type V collagen from acidic mixtures of interstitial collagens is reported. The technique is based on the higher solubility of this collagen type in alkaline KCl-phosphate solution, and the purity of the sample has been monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electron microscopic analyses. The latter reveal that the majority of the molecules are endowed with a thread portion and a terminal knob, and show a low tendency to originate ordered and reproducible polymers at low temperature.
Luparello
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Dipartimento di Biologia Cellulare e dello Sviluppo, Università, Palermo, Italy.
Rizzo
C P
CP
Schillaci
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Pucci-Minafra
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I
D001943:Breast Neoplasms / Q000032:analysis*; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D003094:Collagen / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005348:Fibrocystic Breast Disease / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D011189:Potassium Chloride
D011189:Potassium Chloride; D003094:Collagen