MicroELISA detection of thymosin alpha 1 released in thymic organ cultures.
Using a polyclonal specific rabbit anti-thymosin alpha 1 a highly sensitive enzyme-linked immunosorbent assay (ELISA) has been developed to measure thymosin alpha 1. Production of thymosin alpha 1 was detected in both thymic organ cultures and in mouse serum. The method is rapid (5 h), reproducible and easy to perform.
Sinopoli
N T
NT
Department of Experimental Medicine, 1st Rome University, Italy.
Pesce
A
A
Baldassarre
A M
AM
Trivers
G E
GE
Frati
L
L
Rinaldi-Garaci
C
C
D000818:Animals; D000937:Antigen-Antibody Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D013154:Spleen / Q000378:metabolism; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*
D013947:Thymosin; D000077596:Thymalfasin