[Characteristics of the cytolysin of Legionella pneumophila].
A thermolabile cytolysin was purified from liquid culture of L. pneumophila. Its homogenicity was determined by the Ouchterlony double immunodiffusion and SDS-electrophoresis in polyacrylamide gel. The molecular weight of cytolysin was ca. 37 kDa. Analysis of amino acid composition revealed a high proportion of aromatic, dicarbonic amino acids, and methionine. The minimal cytolytic concentration for CHO cells and erythrocytes was ca. 1 microgram/ml: Purified cytolysin in doses of 10-60 micrograms caused haemorrhage and necrosis when injected i. c. into guinea pigs.
Belyĭ
Iu F
IuF
Vertiev
Iu V
IuV
Tartakovskiĭ
I S
IS
Ezepchuk
Iu V
IuV
Prozorovskiĭ
S V
SV
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003603:Cytotoxins / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D007114:Immunization; D007875:Legionella / Q000472:pathogenicity*; D008970:Molecular Weight; D011817:Rabbits; D012882:Skin Tests
D000596:Amino Acids; D003603:Cytotoxins
Kharakteristika tsitolizina Legionella pneumophila.