Excystation in vitro of Echinostoma liei and E. revolutum (Trematoda) metacercariae.
Metacercariae of Echinostoma liei and E. revolutum were excysted in an alkaline bile-trypsin medium at 41 C in the absence of acid-pepsin pretreatment. After 60 min at a pH of 7.8 or 8.0, excystation of E. liei reached 98%; optimal excystation of E. revolutum occurred at pH 8.2 and was 70% after 60 min. The rate of excystation was very rapid in E. liei, reaching 91% at 30 min, and less rapid in E. revolutum reaching 40% at 30 min. Almost 100% of the E. liei cysts stored for 5.5 mo at 4 C in Locke's 1:1 excysted in the medium, compared to 40% for E. revolutum treated identically.
Fried
B
B
Department of Biology, Lafayette College, Easton, Pennsylvania 18042.
Emili
S
S
D000818:Animals; D003470:Culture Media; D004449:Echinostoma / Q000033:anatomy & histology / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D011309:Preservation, Biological
D003470:Culture Media