title: Role of proteoglycan in the binding of low-density lipoprotein to cultured arterial smooth muscle cells.
abstract: The role of proteoglycans in the binding of 125I-labeled low-density lipoproteins (LDL) to cultured arterial smooth muscle cells was examined. About 60% of cell bound 125I-labeled LDL could be released by unlabeled LDL, heparin, dextran sulfate or proteoglycan. Binding of 125I-labeled LDL decreased by about 50% when incubated in the presence of exogenous arterial proteoglycans. Exposure of cell cultures to rho-nitrophenyl-beta-D xyloside resulted in a 40% decrease in both the amount of 35S-labeled proteoglycan in the cell layer and the 125I-labeled LDL binding, without modifying significantly the cell number and amount of cell layer protein. These data suggest that cell surface and/or cell matrix proteoglycans may influence binding of LDL to either specific receptor or non-receptor sites and thereby play a role in the intracellular deposition of lipid in the arterial wall.
authors: C.S.I.R.O., Division of Protein Chemistry, Parkville, Victoria, Australia.
authors: M C
authors: MC
authors: Horn-Brahimi
authors: M
authors: M
authors: Breton
authors: E
authors: E
authors: Berrou
authors: E
authors: E
authors: Deudon
authors: J
authors: J
authors: Picard
mesh_terms: D001011:Aorta / Q000166:cytology / Q000378:metabolism; D001665:Binding Sites; D002478:Cells, Cultured; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D011509:Proteoglycans / Q000494:pharmacology / Q000502:physiology*
chemical_list: D008077:Lipoproteins, LDL; D011509:Proteoglycans