cDNA cloning and sequencing of a new gene intensely expressed in early differentiation stages of embryonal carcinoma cells and in mid-gestation period of mouse embryogenesis.
By the differential hybridization technique, we isolated a cDNA clone, MK1, whose RNA level increased in early stages of retinoic acid-induced differentiation of embryonal carcinoma cells. The amount of MK1 RNA progressively decreased in the later stages of the differentiation. In mouse embryos, MK1 RNA was abundant in mid-gestation stages (Day 8 to Day 11) and decreased thereafter. The corresponding RNA was 1.0 killobase in size. From the nucleotide sequence, MK1 gene was predicted to code a polypeptide of molecular weight 9,971, which was rich in basic amino acids.
Kadomatsu
K
K
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
Tomomura
M
M
Muramatsu
T
T
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005314:Embryonic and Fetal Development; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013724:Teratoma / Q000235:genetics* / Q000473:pathology
D004247:DNA