Presence of benzodiazepine-like molecules in mammalian brain and milk.
From bovine cerebral cortex extracts, used to isolate n-butyl beta carboline-3-carboxylate (8), fractions active in displacing [3H] flunitrazepam binding were purified and shown to contain benzodiazepine-like molecules. These were recognized by UV spectra, retention time in HPLC, and interaction with a specific monoclonal antibody. Such molecules were localized in synaptic vesicles and cytosol of synaptosomes. Similar molecules were also found in cow milk. The possible dietary origin of these benzodiazepine-like molecules is discussed.
Medina
J H
JH
Instituto de Biología Celular, Facultad de Medicina, Buenos Aires, Argentina.
Peña
C
C
Piva
M
M
Paladini
A C
AC
De Robertis
E
E
D000818:Animals; D001569:Benzodiazepines / Q000302:isolation & purification*; D002417:Cattle; D002540:Cerebral Cortex / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D008892:Milk / Q000032:analysis*; D008970:Molecular Weight; D013572:Synaptic Vesicles / Q000032:analysis; D013574:Synaptosomes / Q000032:analysis
D001569:Benzodiazepines