Excretory/secretory antigen of Toxocara canis: recognition profiles of polyclonal and larvicidal monoclonal antibodies.
Five monoclonal antibodies against the excretory/secretory (E/S) antigen of Toxocara canis were obtained and characterized. Immunoprecipitating activity was demonstrated in an in-vitro microprecipitating assay using live T. canis larvae. Their capacity to kill larvae was also shown in an in-vitro assay. Two zones of reactivity were observed in 7.5 and 12.5% SDS-PAGE (177-77 kD, 43-15 kD) of immunoprecipitates of human and mouse positive polyclonal antisera. The murine monoclonal antibodies showed a common pattern of reactivity with the proteins in the 177-77 kD range.
del Aguila
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C
Departamento de Parasitología, Facultad de Farmacia, Universidad Computense de Madrid, Spain.
Cuellar
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C
Guillén
J L
JL
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins; D007118:Immunoassay; D007814:Larva / Q000276:immunology; D014119:Toxocara / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015801:Helminth Proteins; C045159:TES-26 protein, Toxocara canis