[Molecular and hormone-binding properties of a partially purified preparation of androgen receptors from the liver of male rats].
Liver cytosol of mature male rats was found to contain androgen receptors (AR). These AR were purified 7--10-fold, and their molecular and hormone-binding properties were investigated. It was found that the AR molecules have a sedimentation coefficient of 9.3 +/- 0.15 and 4.7 +/- 0.11 S, Stokes radius of 6.5 +/- 0.25 and 3.2 +/- 0.08 nm, Mr of 263000 and 65700 Da and friction coefficient ratio of 1.55 and 1.22 for low and high ionic strength media, respectively. The values of rate constants of [3H]methyltrienolone (R1881) association with AR and for the dissociation of the complexes formed are equal to (0.66 +/- 0.29). .10(5) M-1 s-1 and to (0.68 +/- 0.08).10(-5) s-1, respectively, whereas those of equilibrium association constant--to (1.4 +/- 0.3).10(9) M-1 at 0-4 degrees C. It was shown that R1881, 5 alpha-dihydrotestosterone and testosterone strongly compete with 3H-R1881 for the binding with AR (as can be judged from the relative competitive activity of 35 nonlabeled hormonal agents); 19-nortestosterone and delta1-testosterone are more weak, whereas 5 alpha-androstandiols, some hydroxy derivatives of testosterone, cyproterone acetate, estradiol and progesterone are moderate competitors. Other natural testosterone, estradiol and progesterone metabolites as well as corticosteroids do not compete or weakly compete with 3H-R1881 for the binding to AR. It is concluded that the properties of AR are similar to those of classical type AR and that they can intermediate most of the direct effects of androgens on the liver.
El'chaninova
S A
SA
Smirnova
O V
OV
Rozen
V B
VB
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D007700:Kinetics; D008024:Ligands; D008099:Liver / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D011869:Radioligand Assay; D051381:Rats; D011944:Receptors, Androgen / Q000302:isolation & purification / Q000378:metabolism*
D000728:Androgens; D008024:Ligands; D011944:Receptors, Androgen
Molekuliarnye i gormonsviazyvaiushchie svoĭstva chastichno ochishchennogo preparata retseptorov androgenov pecheni samtsov krys.