A new method for cloning Giardia lamblia, with a discussion of the statistical considerations of limiting dilution.
This report describes a method of cloning Giardia lamblia by limiting dilution which is simpler than the previously described semisolid agar technique and which may also be applied as an assay of cell viability. A discussion of the basic statistics of limiting dilution, which is applicable to any cell type, and a method of statistically comparing colony-forming efficiencies from different cell populations are included. The colony-forming efficiency (CFE) of this method, when applied to late log-phase cultures, is 72.1 +/- 10.05%. When only cells adherent to the sides of culture vials are cloned, the CFE is 87.1 +/- 9.85%.
Baum
K F
KF
Department of Medicine, University of Colorado Health Sciences Center, Denver 80262.
Berens
R L
RL
Jones
R H
RH
Marr
J J
JJ
D000818:Animals; D003470:Culture Media; D005872:Giardia / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D010274:Parasitology / Q000379:methods*; D013223:Statistics as Topic
D003470:Culture Media