title: Measurement of ferritin in serum by an indirect competitive enzyme-linked immunosorbant assay.
abstract: This indirect competitive enzyme-linked immunosorbant assay (ELISA) for ferritin, unlike other currently available ELISAS, does not require use of an anti-ferritin antibody-enzyme conjugate. Designed for use on microtiter plates, the method has a precision and sensitivity similar to those of other immunoassays. The detection limit is 20 pg of ferritin per test (corresponding to 2.0 micrograms/L in serum samples). Comparison of results obtained on serum from 57 patients by this method with those from a conventional radioimmunoassay gave a correlation coefficient of 0.92.
authors: Liver Unit, King's College Hospital, London, U.K.
authors: R J
authors: RJ
authors: Polson
authors: J G
authors: JG
authors: Kenna
authors: I P
authors: IP
authors: Shears
authors: A
authors: A
authors: Bomford
authors: R
authors: R
authors: Williams
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005293:Ferritins / Q000097:blood*; D006801:Humans; D011863:Radioimmunoassay
chemical_list: D005293:Ferritins