Monoclonal antibody recognition of two subtype forms of protein kinase C in human platelets.
Using a hydroxylapatite column chromatographic technique, we obtained the evidence for two subtype forms of protein kinase C in human platelets. These subtypes had a similar chromatographic property to Type II, Type III protein kinase C from the rabbit brain. In addition, in monoclonal antibodies (MC-1a, 2a, 3a) (1) which reacted with specifically Type I, II, III rabbit brain protein kinase C, respectively, only MC-2a and MC-3a reacted with human platelet protein kinase C. All these brain and platelet subtypes have a similar Km value for ATP, the range being from 8.0 to 20.0 microM and a similar IC50 value with regard to the effect of the protein kinase C inhibitor, H-7. Thus, the possibility that specific functions of platelet may be derived from a deficiency of Type I protein kinase C warrants attention.
Watanabe
M
M
Department of Molecular and Cellular Pharmacology, Mie University School of Medicine, Japan.
Hagiwara
M
M
Onoda
K
K
Hidaka
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification; D000937:Antigen-Antibody Reactions; D001792:Blood Platelets / Q000276:immunology*; D001921:Brain / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011493:Protein Kinase C / Q000097:blood / Q000145:classification* / Q000276:immunology; D011817:Rabbits
D000911:Antibodies, Monoclonal; D011493:Protein Kinase C