Polymerized actin in lymphoid cells: functional interpretation.
Nitrobenzoxadiazol (NBD) phallacidin, an active fluorescent derivative of the actin-binding mushroom toxin phallacidin provides a convenient actin-specific fluorescent label for cellular cytoskeleton structures. Topographical fluorescent microscopy images of lymphoid cells obtained with NBD-phallacidin staining reveal that the major feature of the cellular cytoskeleton characterized by actin are mainly associated with cell membrane, a pattern that correlates strikingly with their DNAse 1 inhibition. Such actin pools may thus be involved in a membrane-associated protein network controlling membrane viscoplastic deformation and cell motility.
Philip
P J
PJ
Laboratory de Pathologie Cellulaire, Faculté de Médecine, Nice, France.
Mely-Goubert
B
B
D000199:Actins / Q000032:analysis*; D000546:Amanitins; D002462:Cell Membrane / Q000032:analysis; D005347:Fibroblasts / Q000032:analysis; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D008214:Lymphocytes / Q000032:analysis*; D011108:Polymers
D000199:Actins; D000546:Amanitins; D005456:Fluorescent Dyes; D011108:Polymers; C028877:7-nitrobenz-2-oxa-1,3-diazole-phallacidin