Monoclonal antibodies to human factor VII: a one step immunoradiometric assay for VII:Ag.
Three mouse monoclonal antibodies (RFF-VII/1, RFF-VII/2, and RFF-VII/3) which bind specifically to different epitopes on human factor VII antigen were raised. Two of the antibodies, RFF-VII/1 and RFF-VII/2, bound strongly to factor VII antigen (VII:Ag), but only RFF-VII/1 and RFF-VII/3 were potent inhibitors of factor VII coagulation activity (VII:C). RFF-VII/1 and RFF-VII/2 were used in a one step, double monoclonal immunoradiometric assay for VII:Ag. This was highly reproducible and detected as little as 0.05U/dl VII:Ag. Values for VII:Ag obtained for plasma samples from normal subjects (n = 20), patients with liver disease (n = 20), patients treated with warfarin (n = 20), and those congenitally deficient in factor VII (n = 7) correlated very well (r = 0.96) with data obtained in a radioimmunoassay using polyclonal rabbit antiserum to factor VII. This simple and sensitive monoclonal antibody based assay offers a convenient method for the detection of VII:Ag in various disease states.
Takase
T
T
Academic Department of Haematology, Royal Free Hospital, London.
Tuddenham
E G
EG
Chand
S
S
Goodall
A H
AH
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D005167:Factor VII / Q000276:immunology*; D005168:Factor VII Deficiency / Q000276:immunology; D006801:Humans; D008107:Liver Diseases / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay / Q000379:methods*; D014859:Warfarin / Q000627:therapeutic use
D000911:Antibodies, Monoclonal; D000941:Antigens; D014859:Warfarin; D005167:Factor VII