A comparison of two ELISAs for detecting HBe markers.
A new enzyme-linked immunosorbent assay (ELISA) kit (Wellcozyme HBeAg/anti-HBe; Wellcome Diagnostics) for detecting hepatitis Be antigen and antibody, the markers by which the infectivity of persons with hepatitis B surface antigenaemia may be inferred, has been compared with the ELISA obtained from the Division of Microbiological Reagents and Quality Control (Public Health Laboratory Service, London U.K.) currently used in this laboratory. The new test may be completed within 2 h compared with about 19 h for the current test. There was agreement between the two assay kits with 106/109 (97%) samples tested. The new test is acceptably sensitive and specific as well as being straightforward to perform.
Sutehall
G M
GM
Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, U.K.
Wreghitt
T G
TG
D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006509:Hepatitis B / Q000175:diagnosis / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006513:Hepatitis B e Antigens / Q000032:analysis*; D006801:Humans
D006510:Hepatitis B Antibodies; D006513:Hepatitis B e Antigens