Influence of ammonium sulfate and sodium chloride on ethyl methanesulfonate-induced chromosomal aberrations and HPRT mutations in V79 hamster cells.
V79 hamster cells were mutagenized with ethyl methanesulfonate (EMS) and immediately afterwards posttreated with hypertonic solutions of sodium chloride or ammonium sulfate. The posttreatment had a clear effect on chromosomal aberrations, but TGr mutations were only enhanced by ammonium sulfate, but not by sodium chloride posttreatment. It is suggested that hypertonic salt posttreatment leads to conformational changes in the DNA, resulting in an increase in TGr mutations and chromosomal aberrations.
Nowak
C
C
Institut für Genetik, Freie Universität Berlin, F.R.G.
D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004347:Drug Interactions; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006982:Hypertonic Solutions; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008168:Lung; D008297:Male; D009154:Mutation; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*
D006982:Hypertonic Solutions; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride; D005020:Ethyl Methanesulfonate; D007041:Hypoxanthine Phosphoribosyltransferase; D000645:Ammonium Sulfate