Electron microscopic visualization of fluorescent microspheres used as a neuronal tracer.
A method is described to visualize rhodamine-labelled microspheres (RLM) at the electron microscopic level using potassium permanganate for negative contrast. Retrogradely labelled nerve cells in the thalamus of the rat and in tissue culture are examined. In addition, it is demonstrated that single beads as small as 50 nm in diameter can be identified with the fluorescent microscope.
Egensperger
R
R
Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, F.R.G.
Holländer
H
H
D000818:Animals; D001931:Brain Mapping; D005456:Fluorescent Dyes; D008854:Microscopy, Electron / Q000379:methods*; D008863:Microspheres; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D014793:Visual Cortex / Q000033:anatomy & histology* / Q000648:ultrastructure
D005456:Fluorescent Dyes