[Activation of the production of cytotoxic factors by mouse spleen cells exposed to the combined action of lipopolysaccharide and muramyl dipeptide in vitro].
Lipopolysaccharide (LPS) and muramyl dipeptide (MDP) stimulated murine splenocytes in vitro to produce cytotoxic factors (CF) that killed target cells L-929. This effect was synergic at LPS dose of 10 ng/ml and MDP dose of 10 micrograms/ml. CF production started 2 hours after spleen cell activation and was maximum in 6 hours. CF were produced by macrophages as well as by lymphocytes stimulated by LPS, MDP or their combination. However, synergic effect of immunomodulators was registered only if nonfractionated spleen cells were stimulated during 24 hours. Lymphocytes depleted on T cells did not lack the ability to generate CF upon activation. In addition, LPS and MDP activated synergically the production of interleukin-I by spleen cells in vitro.
Rakhmilevich
A L
AL
Rakhimova
M S
MS
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000008:administration & dosage*; D000818:Animals; D003603:Cytotoxins / Q000096:biosynthesis*; D004357:Drug Synergism; D005260:Female; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D013268:Stimulation, Chemical
D003603:Cytotoxins; D008070:Lipopolysaccharides; D000119:Acetylmuramyl-Alanyl-Isoglutamine
Aktivatsiia produktsii tsitotoksicheskikh faktorov kletkami selezenki mysheĭ pri sochetannom deĭstvii lipopolisakharida i muramildipeptida in vitro.