Sciatic nerve neuropathy target esterase. Methods of assay, proximo-distal distribution and regeneration.
Some organophosphorus compounds (OP) induce a delayed polyneuropathy (OPIDP) which is initiated by the phosphorylation of the so-called neuropathy target esterase (NTE). In this work some aspects of hen sciatic nerve NTE are studied. The assay method is reported and modifications are discussed and a combined method proposed. Proximo-distal distribution showed a significant difference from proximal (100 +/- 10%) to distal (69 +/- 9%) fragments, in accordance with reported data. The time course of in vivo regeneration after a single TOCP dose (200 mg/kg, post oral) showed some differences when compared with hen brain NTE. Sciatic nerve NTE showed a delay of 2-3 days before regeneration but then regenerated faster (74% activity at day 7) than brain NTE (50% activity at day 7). A slower rate of regeneration of distal than proximal segments has been suggested to explain higher sensitivity of distal segments [3], however in this work no significant differences were observed in the rate of regeneration when comparing proximal and distal fragments.
Barril
J B
JB
Departamento de Neuroquímica, Universidad de Alicante, Spain.
Vilanova
E
E
Pellin
M C
MC
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000378:metabolism*; D002645:Chickens; D005260:Female; D007531:Isoflurophate / Q000031:analogs & derivatives / Q000633:toxicity; D009416:Nerve Regeneration / Q000187:drug effects*; D009943:Organophosphorus Compounds / Q000633:toxicity*; D010261:Paraoxon / Q000633:toxicity; D012584:Sciatic Nerve / Q000187:drug effects / Q000201:enzymology*; D013450:Sulfones / Q000633:toxicity
D009943:Organophosphorus Compounds; D013450:Sulfones; D007531:Isoflurophate; C005238:mipafox; C056032:phenylsulfonylfluoride; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase; D010261:Paraoxon