title: The alpha-mannosidase from Canavalia ensiformis seeds: chemical and kinetic properties and action on animal lymphocytes.
abstract: The alpha-mannosidase from Canavalia ensiformis was characterized with respect to molecular mass, glycoprotein nature, amino-acid composition, enzymic properties and action on animal cells. The enzyme is composed of two pairs of subunits (molecular mass 44 and 66 kDa) which form a tetramer (220 kDa). The larger subunit is glycosylated, the smaller one is not. Both subunits have similar amino-acid compositions. The larger subunit contains a surplus of alanine, aspartic acid/asparagine, histidine, phenylalanine and tyrosine, the smaller one a surplus of glutamic acid/glutamine, serine and threonine. The enzyme is subject to product inhibition by mannose. It stimulates the proliferation of B-lymphocytes from nude mice.
authors: Institut für Pharmazie und Lebensmittelchemie der Universität Würzburg.
authors: W
authors: W
authors: Einhoff
authors: H
authors: H
authors: Rüdiger
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D046911:Macromolecular Substances; D008361:Mannosidases / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude; D008934:Mitogens; D008970:Molecular Weight; D012639:Seeds / Q000201:enzymology*; D043323:alpha-Mannosidase
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D008934:Mitogens; D008361:Mannosidases; D043323:alpha-Mannosidase