Characterization and hormonal regulation of protein synthesis by the murine epididymis.
Protein synthesis and secretion were examined in vitro by incubating minced tissue with [35S]methionine. The incorporation of label into tissue plus medium was linear for the 5 h of incubation. The percentage of available label incorporated into protein increased with the weight of tissue used. Approximately 13% of the label incorporated appeared in the medium after 5 h of incubation. Release of radioactive protein into the medium was characterized by an initial slow release (1-2 h) followed by a more rapid linear release between 3 and 5 h. Polyacrylamide gel electrophoresis revealed that the pattern of radioactive proteins present in the medium was different from and less complex than the tissue proteins. Substantial differences in protein patterns from different epididymal regions could be detected. The caput epididymidis was particularly active in secreting proteins characteristic of this region, whereas the corpus and cauda synthesized and secreted similar proteins. At least one of these proteins characteristic of the caput is stabilized by disulphide bonds. Short-term (9 day) castration resulted in reduced synthesis and secretion of several of these epididymal proteins. Testosterone administered after 9 days of castration reinitiated synthesis of some but not all of these epididymal proteins.
Holland
M K
MK
Department of Obstetrics & Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Orgebin-Crist
M C
MC
D000728:Androgens / Q000502:physiology*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009919:Orchiectomy; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology
D000728:Androgens; D011506:Proteins; D013739:Testosterone; D008715:Methionine