title: 6-Nitrocholesterol inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase and tumor cell growth.
abstract: 6-Nitrocholesterol has been shown to cause a 50% reduction in the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in animal cells in culture at 1.9 microM and it has relative binding affinity for the cytosolic oxysterol binding protein of 357 nM in cell-free extracts from the same cell line. In addition, significant cytotoxicity was observed when this sterol was incubated with hepatoma and lymphoma cells in culture.
authors: Department of Chemistry, Auburn University, Alabama 36849.
authors: E J
authors: EJ
authors: Parish
authors: K L
authors: KL
authors: Dixon
authors: S
authors: S
authors: Chitrakorn
authors: F R
authors: FR
authors: Taylor
authors: B
authors: B
authors: Luu
authors: G
authors: G
authors: Ourisson
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D051379:Mice; D051381:Rats; D011987:Receptors, Steroid / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000098825:Oxysterol Binding Proteins
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins; C056245:6-nitrocholesterol; D002784:Cholesterol