Reciprocal age-dependent pattern of two neuronal markers, tetanus toxin and neuron-specific enolase, in postnatal rat sensory and sympathetic neurons.
Tetanus toxin (TT) and antibodies to neuron-specific enolase (NSE) were used to study the developmentally regulated expression of two neuronal markers in isolated and short-term cultured rat sensory and sympathetic neurons at different postnatal ages. The results show a reciprocal pattern of TT binding and NSE-immunoreactive neurons during postnatal maturation: there is a gradual transition from TT-positive, NSE-negative to TT-negative, NSE-positive phenotypic expressions. We conclude that TT may serve as a neuronal marker for newborn, anti-NSE antibodies as markers for adult neurons, and a combination of both for adolescent neurons.
Grothe
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C
Department of Anatomy and Cell Biology, University of Marburg, F.R.G.
Unsicker
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D000320:Adrenergic Fibers / Q000201:enzymology / Q000502:physiology*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000201:enzymology / Q000254:growth & development*; D007150:Immunohistochemistry; D009475:Neurons, Afferent / Q000201:enzymology / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013744:Tetanus Toxin / Q000032:analysis / Q000502:physiology*
D013744:Tetanus Toxin; D010751:Phosphopyruvate Hydratase