Pathogenesis of Campylobacter fetus infections. Failure of encapsulated Campylobacter fetus to bind C3b explains serum and phagocytosis resistance.
Campylobacter fetus ssp. fetus strains causing systemic infections in humans are highly resistant to normal and immune serum, which is due to the presence of high molecular weight (100,000, 127,000, or 149,000) surface (S-layer) proteins. Using serum-resistant parental strains (82-40 LP and 23D) containing the 100,000-mol wt protein and serum-sensitive mutants (82-40 HP and 23B) differing only in that they lack the 100,000-mol wt protein capsule, we examined complement binding and activation, and opsono-phagocytosis by polymorphonuclear leukocytes. C3 consumption was similar for all four strains but C3 was not efficiently bound to 82-40 LP or 23D even in the presence of immune serum, and the small amount of C3 bound was predominently the hemolytically inactive iC3b fragment. Consumption and binding of C5 and C9 was significantly greater for the unencapsulated than the encapsulated strains. Opsonization of 82-40 HP with heat-inactivated normal human serum caused greater than 99% killing by human PMN. Similar opsonization of 82-40 LP showed no kill, but use of immune serum restored killing. Findings in a PMN chemiluminescence assay showed parallel results. Association of 32P-labeled 82-40 HP with PMN in the presence of HINHS was 19-fold that for the 82-40 LP, and electron microscopy illustrated that the difference was in uptake rather than in binding. These results indicate that presence of the 100,000-mol wt protein capsule on the surface of C. fetus leads to impaired C3b binding, thus explaining serum resistance and defective opsonization in NHS, mechanisms that explain the capacity of this enteric organism to cause systemic infections.
Blaser
M J
MJ
Infectious Disease Section, Veterans Administration Medical Center, Denver, Colorado 80220.
Smith
P F
PF
Repine
J E
JE
Joiner
K A
KA
D001770:Blood Bactericidal Activity; D002169:Campylobacter Infections / Q000209:etiology* / Q000276:immunology; D002168:Campylobacter fetus / Q000276:immunology* / Q000472:pathogenicity / Q000648:ultrastructure; D003176:Complement C3 / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D003182:Complement C5 / Q000276:immunology / Q000378:metabolism; D003186:Complement C9 / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins; D010587:Phagocytosis; D014774:Virulence
D003176:Complement C3; D003182:Complement C5; D003186:Complement C9; D009895:Opsonin Proteins