Nasal eosinophilia in children: its use in the nasal allergen provocation test.
This pediatric cytological and clinical study aimed at assessing the value of nasal eosinophilia during nasal provocation tests for identifying an offending allergen. The population studied comprised 50 children aged from 4 to 18 yr; 39 of these had well-characterized allergic rhinitis, which in 21 cases was combined with asthma, and the remaining 11 had nonatopic chronic rhinitis. Nasal secretions, collected by nose blowing, were stained with May-Grünwald-Giemsa or Wright stain. The percentage of nasal eosinophils was obtained by examining the cells in the whole slides. Counts were carried out on secretions collected before challenge, after insufflation of saline solution (for verification), and 40 min after insufflation into each nostril of an allergen (housedust mite extract). The nasal provocation test was considered positive when insufflation of the allergen increased nasal eosinophilia by more than 10%, provided that the prechallenge proportion of eosinophils was less than 50%. No increase in specific bronchial resistance was noted. These results indicate that nasal provocation tests are safe, even in asthmatic children.
Jean
R
R
Service de Pneumo-Allergologie Infantile, Hôpital des Enfant Malades, Paris, France.
Lellouch-Tubiana
A
A
Brunet-Langot
D
D
Scheinmann
P
P
Pfister
A
A
D000293:Adolescent; D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000276:immunology / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D003581:Cytodiagnosis; D004391:Dust; D004804:Eosinophils / Q000473:pathology*; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000473:pathology*; D007958:Leukocyte Count; D008297:Male; D008925:Mites / Q000276:immunology; D009297:Nasal Mucosa / Q000378:metabolism; D009299:Nasal Provocation Tests; D012220:Rhinitis / Q000276:immunology / Q000473:pathology
D000485:Allergens; D004391:Dust