Tunicamycin-treated rat heart cell cultures synthesize an inactive nonreleasable lipoprotein lipase.
Cells isolated from newborn rat hearts were cultured in the presence of 100 mM Hepes (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). Lipoprotein lipase activity was present in an intracellular and heparin-releasable pool and was also secreted into the culture medium. Treatment of the cultures with 5 micrograms/ml tunicamycin caused almost complete loss of lipoprotein lipase activity in all three compartments. In control cultures, immunoblotting of lipoprotein lipase derived from all three pools revealed a single band of lipoprotein lipase with an apparent Mr of 56,000. In the tunicamycin-treated cultures, the enzyme appeared only intracellularly and had an apparent Mr of 49,000. No immunoreactive enzyme was found in the medium. Thus, glycosylation of lipoprotein lipase in heart cell cultures is mandatory for enzyme activity and translocation from an intracellular to the heparin-releasable pool and for secretion into the medium.
Friedman
G
G
Department of Medicine B, Hadassah University Hospital, Jerusalem, Israel.
Chajek-Shaul
T
T
Etienne
J
J
Stein
O
O
Stein
Y
Y
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004789:Enzyme Activation; D008071:Lipoprotein Lipase / Q000096:biosynthesis*; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D014415:Tunicamycin / Q000494:pharmacology*
D003470:Culture Media; D014415:Tunicamycin; D008071:Lipoprotein Lipase