Energy metabolism of sea urchin spermatozoa, with phosphatidylcholine as the preferred substrate.
Phosphatidylcholine content in the spermatozoa of the sea urchin, Hemicentrotus pulcherrimus, decreased rapidly during incubation with sea water. Sea urchin sperm contained approx. 85% phospholipid in total lipid. Phosphatidylcholine (PC) was the principal lipid. Other phospholipids, however, remained at constant levels during incubation. Although the free fatty acid content gradually increased following dilution of dry sperm in sea water, the amounts of triacylglycerol and cholesterol ester were extremely low. Analysis by gas-liquid chromatography indicated most of fatty acid moieties in PC to be polyenoic. PC composed in part of unsaturated fatty acids was consumed to a greater extent during incubation than that consisting of saturated fatty acids. Furthermore, 1-palmitoyl-2-[1-14C]linoleoylphosphatidylcholine was transformed to 14C-labelled free fatty acid in a subcellular system. Thus, possibly, phospholipase A2 is present in sea urchin sperm. Also, [1-14C]oleic acid was immediately oxidized to 14CO2 by sperm. It is thus concluded that sea urchin sperm use phosphatidylcholine as a substrate for energy metabolism.
Mita
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M
Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan.
Ueta
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N
D000818:Animals; D004734:Energy Metabolism; D005227:Fatty Acids / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000032:analysis; D010740:Phospholipases / Q000378:metabolism; D012016:Reference Values; D012617:Sea Urchins; D013094:Spermatozoa / Q000378:metabolism*
D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D009829:Oleic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D019301:Oleic Acid; D010740:Phospholipases