Glomeruli from ischemic rat kidneys produce increased amounts of platelet activating factor.
The production of Platelet Activating Factor (PAF-acether) by glomeruli isolated from rats subjected to a 60 min renal ischemia has been studied. PAF-acether has been purified by Sep-Pak columns and measured by its ability to release serotonin from previously loaded rabbit platelets. Glomeruli from ischemic kidneys had higher amounts of PAF-acether activity than glomeruli from sham operated rats. These data suggest a role for PAF-acether in the renal functional alterations induced by renal ischemia.
López-Farré
A
A
Laboratory of Renal Physiopathology, Fundación Jiménez Diaz-CSIC, Madrid, Spain.
Torralbo
M
M
López-Novoa
J M
JM
D000818:Animals; D066298:In Vitro Techniques; D007511:Ischemia / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism*; D007700:Kinetics; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D051381:Rats; D012016:Reference Values; D012079:Renal Circulation; D013929:Thromboxane B2 / Q000096:biosynthesis
D010972:Platelet Activating Factor; D013929:Thromboxane B2