Comparison of features of carcinogen-transformed human cells in vitro with sarcoma-derived cells.
To define characteristics of chemically transformed phenotypes during and after progression to neoplasia and to assess their relationship to those phenotypes expressed by surgically removed sarcoma lesions, we compared the characteristics in the following manner. We investigated: (1) alterations in growth patterns; (2) anchorage-independent growth; (3) reactivity with monoclonal antibodies directed against surface antigen; (4) invasiveness in embryonic chick skin; (5) tumorigenicity in nude mice; and (6) karyology. Fifty different sarcoma cell lines were examined which exhibited different rates and absolute numbers of population doublings. With one exception, all sarcoma cell lines exhibited a finite life span ranging from 60 to 100 population doublings. Populations of these cells that exhibited anchorage-independent growth in soft agar also reacted positively with a monoclonal antibody (MoAb) 345.134S directed against a 115K-GP cell surface glycoprotein. Similarly, chemically transformed cells that grew in soft agar also reacted with the MoAb 345.134S, whereas cells with an inability to grow in soft agar did not. Cell lines established from human sarcoma and from chemically transformed human fibroblasts that reacted positively with the MoAb 345.134S were invasive for embryonic chick skin and formed tumors in nude mice. The selection medium used during culture of the carcinogen-treated cells resulted in the appearance of an altered phenotype that after at least 16 population doublings exhibited characteristics common to those cells derived from human sarcomas.
Milo
G E
GE
Department of Physiological Chemistry and Comprehensive Cancer Center, Ohio State University, Columbus 43210.
Casto
B
B
Ferrone
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012509:Sarcoma / Q000473:pathology*; D012867:Skin / Q000166:cytology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface