Design of the immunogen and label for use in a fluoroimmunoassay for paracetamol.
The design of immunogens for raising antisera containing antibodies specifically directed against paracetamol is discussed. A derivative of the drug with minimal structural alteration was synthesised, coupled to the carrier protein key-hole limpet haemocyanin, and used to immunise sheep. The evaluation of the resulting antisera is described. Preparation of a fluorescent label with reduced bridge-binding then allowed the development of a polarisation fluoroimmunoassay for paracetamol in buffer, suitable for conversion to a direct serum assay.
Gallacher
G
G
Department of Chemical Pathology, St Bartholomews Hospital, West Smithfield, London, UK.
Coxon
R
R
Landon
J
J
Rae
C J
CJ
Abukinesha
R
R
D000082:Acetaminophen / Q000031:analogs & derivatives / Q000097:blood* / Q000276:immunology; D000627:Aminophenols / Q000276:immunology; D005452:Fluoresceins; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods
D000627:Aminophenols; D005452:Fluoresceins; C055466:acetaminophen hemisuccinate; D000082:Acetaminophen; C055465:4-caprylamidophenol; D006433:Hemocyanins