The relationship of embryotoxicity to disposition of 2-methoxyethanol in mice.
Paw development of CD-1 mice is uniquely sensitive to 2-methoxyethanol (ME) given by gavage (po) on gestation day (gd) 11 (copulation plug day = gd 0). The relation between induction of paw dysmorphogenesis and disposition of po ME (3.3 or 4.6 mmol/kg) in the maternal and conceptus compartments was investigated. The expression of digit malformations depends on metabolism of ME to methoxyacetic acid (MAA). ME and MAA were equipotent in causing teratogenicity. Alcohol dehydrogenase (ADH) catalyzes the initial rate-limiting oxidation that leads to embryotoxicity. The ADH inhibitor 4-methylpyrazole (0.12 or 1.2 mmol/kg) or ethanol (43.3 mmol/kg, single dose concomitant with ME or additional ethanol 5 and 10 hr later) reduced the incidence of malformations 60-100%, depending on the dosing regimen. Elimination of 14C from 1,2-14C-ME occurred predominantly via urine where 80% of a teratogenic dose was excreted and 6% appeared in CO2. Oxidation of ME to MAA was nearly complete after 1 hr when approximately 90% of 14C in maternal plasma and conceptus coeluted with authentic 14C-MAA upon HPLC. 14C-MAA levels in embryos were 1.2 X those in plasma 1 and 6 hr after dosing, although by 6 hr concentrations had declined to approximately 50% of 1-hr values. Concomitant ethanol did not affect 14C kinetics as measured in maternal blood after oral 14C-ME, but retarded ME conversion to MAA by about 2 hr. Furthermore, embryo 14C-MAA levels then reached only 50% of the peak in embryos from dams dosed with ME alone, an effect that coincided with less 14C incorporation into macromolecules synthesized by the embryo within 6 hr. These data imply that the attenuation of digit malformations by concomitant ethanol may be explained by changes in MAA disposition. However, delayed ethanol (5 and 10 hr after 3.3 mmol ME/kg) reduced teratogenicity by 25%, although MAA was present in the embryo up to 5 hr. Dams given 14C-MAA by iv injection had higher 14C blood levels than after MAA po but their offspring had fewer digit malformations. Peak and steady-state plasma levels of MAA as well as embryo concentrations of the chemical do not appear to determine the embryotoxic outcome whereas further metabolism of MAA does.
Sleet
R B
RB
Department of Cellular and Molecular Toxicology, Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709.
Greene
J A
JA
Welsch
F
F
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000085:Acetates / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity; D000444:Aldehyde Dehydrogenase / Q000494:pharmacology; D000818:Animals; D002250:Carbon Radioisotopes; D004622:Embryo, Mammalian / Q000187:drug effects*; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology; D005026:Ethylene Glycols / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D000077604:Fomepizole; D008297:Male; D051379:Mice; D010920:Placenta / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology
D000085:Acetates; D002250:Carbon Radioisotopes; D005026:Ethylene Glycols; D011720:Pyrazoles; D000431:Ethanol; D000077604:Fomepizole; D000444:Aldehyde Dehydrogenase; C005219:methyl cellosolve; C013598:methoxyacetic acid