Inhibitory activity of (E)-5-(2-bromovinyl)-2'-deoxyuridine on the salmonid herpesviruses, Oncorhynchus masou virus (OMV) and Herpesvirus salmonis.
The highly potent and selective anti-herpesvirus agent, (E)-5-(2-bromovinyl)-2'deoxyuridine (BVdU), was examined for its inhibitory effect on the salmonid herpesviruses Oncorhynchus masou virus (OMV) and Herpesvirus salmonis (H. salmonis). Minimum inhibitory concentrations (MIC) of BVdU for OMV and H. salmonis were 1.25 and 3.0 micrograms/ml, respectively; these values were equal to or higher than those obtained for acyclovir or cytarabine. OMV DNA polymerase activity was reduced in a dose-dependent fashion by BVdU 5'-triphosphate (BVdUTP) within the concentration range of 3 to 30 microM. However, BVdUTP could also be substituted for the natural substrate, TTP, in the OMV DNA polymerase assay. It is postulated that the inhibitory action of BVdU on the salmonid herpesviruses is more or less similar to that on other herpesviruses and resides with respect to the inhibition of the virus DNA polymerase activity as well as incorporation of BVdU into the viral DNA.
Kimura
T
T
Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University.
Nishizawa
T
T
Yoshimizu
M
M
De Clercq
E
E
D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D006066:Gonads / Q000166:cytology / Q000187:drug effects / Q000382:microbiology*; D006564:Herpesviridae / Q000187:drug effects*; D012487:Salmonidae / Q000382:microbiology*; D014740:Vidarabine / Q000494:pharmacology; D014779:Virus Replication
D000998:Antiviral Agents; C020235:brivudine; D004259:DNA-Directed DNA Polymerase; D014740:Vidarabine; D001973:Bromodeoxyuridine; D000212:Acyclovir