title: Induction of histidine decarboxylase of rat basophilic leukemia (2H3) cells stimulated by higher oligomeric IgE or phorbol myristate acetate.
abstract: When rat basophilic leukemia (2H3) cells were stimulated by higher oligomer, the chemically cross-linked oligomers of IgE, in the presence of calcium the activity of histidine decarboxylase (HDC, L-histidine carboxylase, E.C.4.1.1.22), a histamine-forming enzyme, was increased by 1 hr, reaching maximum activity by 2 hr, and returning to the original level by 8 hr. A similar increase in enzyme activity was observed in cells treated with phorbol myristate acetate (PMA) or oleoyl-acetylglycerol (OAG), which are known activators of protein kinase C. Removal of calcium from medium abolished the increase in HDC activity in response to higher oligomer but not that induced by PMA or OAG, suggesting that the increase in HDC activity may be mediated by protein kinase C. The increase in the HDC activity probably required induction of enzyme synthesis, because it was prevented by cycloheximide.
authors: Department of Pharmacology I, Tohoku University School of Medicine, Miyagi, Japan.
authors: K
authors: K
authors: Maeyama
authors: Y
authors: Y
authors: Taguchi
authors: M
authors: M
authors: Sasaki
authors: H
authors: H
authors: Wada
authors: M A
authors: MA
authors: Beaven
authors: T
authors: T
authors: Watanabe
mesh_terms: D000818:Animals; D001491:Basophils; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D004790:Enzyme Induction; D005989:Glycerides / Q000494:pharmacology; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D007073:Immunoglobulin E / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000201:enzymology*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
chemical_list: D005989:Glycerides; D007073:Immunoglobulin E; C005953:monoolein; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase; D013755:Tetradecanoylphorbol Acetate