Protein synthesis by neuroblastoma cells is enhanced by exposure to burst-mode ultrasound cavitation.
We observed enhancement of protein synthesis, compared to control cells, 24 h after exposing C1300 neuroblastoma cells (N2A) in rotating tubes at 37 degrees C to 1-MHz ultrasound tone bursts [1:1; durations from 6 to 600 ms; 3.4 W/cm2 spatial-peak burst-average intensity; 5 min total treatment duration (on + off periods)]. Protein synthesis was measured by uptake of 3H-leucine and normalized for cell proliferation by measured uptake of 14C thymidine. The similarity between results for enhanced cavitation-stimulated iodine-release reported by Ciaravino and our results for cellular protein synthesis suggests that cavitation causes this biological effect.
Edmonds
P D
PD
Bioengineering Research Laboratory, SRI International, Menlo Park, CA 94025.
Ross
P
P
D000162:Acoustics; D000818:Animals; D002460:Cell Line; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009447:Neuroblastoma / Q000378:metabolism*; D014465:Ultrasonics
D009363:Neoplasm Proteins