Effects of human alpha-interferon on granulocyte-macrophage progenitor cells (CFU-GM) in vitro.
Two preparations of human interferon (IFN)-alpha were assessed for their influence on granulocyte-macrophage progenitor cells (CFU-GM) in vitro. Both highly purified human IFN-alpha Ly and recombinant IFN-alpha 2a suppressed CFU-GM colony formation in a dose-dependent manner using low-density bone-marrow target cells. Suppression of CFU-GM colony formation was accompanied by an increase in clusters. However, depletion of monocytes, T lymphocytes and B lymphocytes from low-density bone-marrow cells resulted in insensitivity of progenitor cells to IFN-alpha. These results demonstrate that the effects of human IFN-alpha on myeloid progenitor cells (CFU-GM) are mediated by accessory cells within the bone marrow.
Schwamborn
J S
JS
Medizinische Klinik I, Universitäts- und Polikliniken, Homburg/Saar, Federal Republic of Germany.
Nickels
R M
RM
Katthagen
B D
BD
Pees
H W
HW
Scheurlen
P G
PG
D002455:Cell Division / Q000187:drug effects; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology
D007370:Interferon Type I; D011994:Recombinant Proteins