title: Pyruvic-acid-containing polysaccharide in the cell wall of Bacillus polymyxa AHU 1385.
abstract: Three acidic polymer fractions with molecular masses of about 16 kDa, 35 kDa and 70 kDa were isolated from lysozyme digests of N-acetylated cell walls of Bacillus polymyxa AHU 1385 by ion-exchange chromatography and gel chromatography. These fractions, containing mannosamine, glucosamine and pyruvic acid in a molar ratio of about 1:1:1 together with glycopeptide components, were characterized as polysaccharide-linked glycopeptides with one, two and more polysaccharide chains. On the other hand, treatment of the cell walls with glycine/HC1 buffer, pH 2.5, at 100 degrees C for 10 min followed by separation of water-soluble products on ion-exchange chromatography gave three polysaccharide fractions, PS-I-III, which contained different amounts of pyruvic acid (0,0.6 and 0.9 residue/mannosamine residue) along with equimolar amounts of mannosamine and glucosamine. Pyruvate-free polysaccharides similar to PS-I were also obtained from PS-II, PS-III and polysaccharide-linked glycopeptides by treatment with 10 mM HC1 at 100 degrees C for 1 h. Results of analyses of these polysaccharide preparations by 1H-NMR and 13C-NMR measurement and methylation, together with data from characterization of fragments obtained by hydrogen fluoride hydrolysis, lead to the most likely structure, ----3)[4,6-O-(1-carboxyethylidene)]ManNAc(beta 1----4)GlcNac(beta 1----, for the acidic polysaccharide of this strain.
authors: Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Japan.
authors: N
authors: N
authors: Kojima
authors: S
authors: S
authors: Kaya
authors: Y
authors: Y
authors: Araki
authors: E
authors: E
authors: Ito
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis; D001407:Bacillus / Q000032:analysis*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D006595:Hexosamines / Q000032:analysis; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011773:Pyruvates / Q000032:analysis*; D019289:Pyruvic Acid
chemical_list: D006595:Hexosamines; D011135:Polysaccharides, Bacterial; D011773:Pyruvates; D019289:Pyruvic Acid; D000117:Acetylglucosamine; C002022:N-acetylmannosamine