[Improvement of a chromosomal technic for trophoblast biopsy. Apropos of 200 cases].
The authors report their experience of 200 cases of prenatal diagnosis by biopsy of chorionic villi. Because they could not consistently obtain good quality chromosome banding, they propose a modification of the conventional direct method. The addition of methanol in sufficient proportions to the nuclear suspension makes it possible to obtain spontaneous spreading on slides heated to 65 degrees C and eliminates the problems of mechanical spreading.
Saura
R
R
Laboratoire de Cytogénétique, Maternité Pellegrin, Bordeaux.
Longy
M
M
Horowitz
J
J
Vergnaud
A
A
D000328:Adult; D001706:Biopsy / Q000379:methods*; D002824:Chorionic Villi / Q000648:ultrastructure*; D002871:Chromosome Banding / Q000379:methods*; D005260:Female; D006801:Humans; D000432:Methanol; D011296:Prenatal Diagnosis
D000432:Methanol
Biopsie de trophoblaste amélioration de la technique chromosomique. A propos de 200 cas.