title: Differential sensitivity to natural cell-mediated cytotoxicity of two rat colon adenocarcinoma variants differing in their tumorigenicity: identification of the effector cells as natural killer cells.
abstract: DHD/K12 TRb (PROb) and DHD/K12 TSb (REGb) are two cancer cell variants originating from the same rat colon adenocarcinoma. They differ in their tumorigenicity: when inoculated into syngeneic BDIX rats, PROb cells induce progressive tumors whereas REGb cells induce tumors which always regress. As previously described, there is an inverse relation between their tumorigenicity and their susceptibility to NCMC mediated by syngeneic spleen or peripheral blood lymphocytes: PROb cells are significantly less sensitive to NCMC than REGb cells. This suggests a role for NCMC in the regression of REGb tumors. In this work the BDIX NCMC effector cells active in vitro against REGb cells were identified as NK cells according to four criteria: (1) efficacy in a 4-h 51Cr release assay, (2) sensitivity to anti-asGM1 antibody plus complement, (3) LGL morphology, and (4) ability to bind with the same affinity REGb and YAC-1 cells. In spleen, these NK cells were heterogeneous with respect to their asGM1 surface density and their morphology. PROb cells were not lysed by these NK cells in a short-term cytotoxicity assay, but only in a 16-h assay. It was shown that PROb and REGb cells were bound with the same affinity by NK cells, thus they certainly differ in their ability to resist to NK lytic mechanisms. This difference could play a role in the different tumorigenicity of the two variants.
authors: Groupe de Recherche sur les Tumeurs Digestives, INSERM U.252, Dijon, France.
authors: H
authors: H
authors: Pelletier
authors: N O
authors: NO
authors: Olsson
authors: C
authors: C
authors: Fady
authors: D
authors: D
authors: Reisser
authors: P
authors: P
authors: Lagadec
authors: J F
authors: JF
authors: Jeannin
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001667:Binding, Competitive; D003110:Colonic Neoplasms / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D009365:Neoplasm Regression, Spontaneous; D009374:Neoplasms, Experimental / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D006028:Glycosphingolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D003165:Complement System Proteins