Liquid chromatographic determination of penicillins by postcolumn alkaline degradation using a hollow-fiber membrane reactor.
A high-performance liquid chromatographic method using a hollow-fiber membrane reactor is described for the determination of penicillins. This method involves separation of penicillins on a C18 column, postcolumn reaction with sodium hydroxide and mercury (II) chloride introduced into the main flow stream using sulfonated hollow-fiber membrane reactors immersed in each solution (4 M sodium hydroxide and 3 X 10(-2) M mercury (II) chloride plus 10(-2) M nitric acid), and detection at 290 nm based on the uv absorbance of the degradation products. At penicillin concentrations of 5 micrograms/ml, within- and between-run precisions (relative standard deviation) were 0.24-2.39 and 1.19-4.13%, respectively. The detection limits of the proposed method were 1-5 ng at a signal-to-noise ratio of 3. The method was applied to assays of ampicillin and its metabolites in human serum and urine.
Haginaka
J
J
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Wakai
J
J
D000667:Ampicillin / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004867:Equipment Design; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D008627:Mercuric Chloride; D010406:Penicillins / Q000032:analysis*; D012972:Sodium Hydroxide
D008567:Membranes, Artificial; D010406:Penicillins; D008627:Mercuric Chloride; D012972:Sodium Hydroxide; D000667:Ampicillin