An improved purification method for cytosolic malate dehydrogenase from several sources.
A new purification method for cytosolic malate dehydrogenases from several sources has been developed. The procedure, employing chromatographies on 5'AMP-Sepharose, DEAE-Sephacel and Blue-Sepharose, allows for a rapid isolation of the enzyme (approximately 40 hours), in large quantities, with good yields (45-54%). The specific activity of final preparations were around 1300 I.U./mg and were judged homogeneous by polyacrylamide gradient gel and sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance size exclusion chromatography and isoelectric focusing.
Domènech
C
C
Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.
Abante
J J
JJ
Bozal
F X
FX
Mazo
A
A
Cortés
A
A
Bozal
J
J
D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D007525:Isoelectric Focusing; D008099:Liver / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000302:isolation & purification*; D008970:Molecular Weight; D013848:Thigh / Q000201:enzymology
D008291:Malate Dehydrogenase