Agonist-induced changes of platelet tubulin phosphorylation.
Changes in the phosphorylation of platelet tubulin were analyzed as a function of platelet activation. Non-activated platelets incubated with [32P]-phosphate showed multiple peaks of radioactivity when solubilized platelet proteins were analyzed by SDS-polyacrylamide gradient gel electrophoresis. Both tubulin monomers were found to be phosphorylated. Agonistic stimulation (thrombin or 1,2-diacylglycerol) resulted in a lowering of the phosphate incorporation into alpha- and beta-tubulin. Such changes we believe are important in the modulation of the reversible polymerization-depolymerization of platelet tubulin that occurs in the course of the agonistic stimulation of platelets.
Berry
S
S
Division of Hematology/Oncology, Memorial Hospital of RI, Pawtucket.
Dawicki
D
D
Steiner
M
M
D001345:Autoradiography; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002846:Chromatography, Affinity; D004075:Diglycerides / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007784:Lactoperoxidase / Q000378:metabolism; D010766:Phosphorylation; D011108:Polymers; D013917:Thrombin / Q000494:pharmacology; D014404:Tubulin / Q000097:blood*
C011439:1,2-diacylglycerol; D004075:Diglycerides; D011108:Polymers; D014404:Tubulin; D007784:Lactoperoxidase; D013917:Thrombin