Spin-echo 1H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte.
Human erythrocytes were subjected to oxidative stress using arsenicals. The study is a model for thiol-related redox processes observed in the etiology of diseases such as rheumatoid arthritis. Spin-echo NMR spectroscopy of the living cell was used to monitor the cellular biochemistry. Oxidation of glutathione and the first demonstrated response of ergothioneine to a chemical stimulus in a cell were observed. The reversible nature of the ergothioneine response is interpreted as an environmental rather than a chemical change.
Reglinski
J
J
Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, Scotland.
Smith
W E
WE
Sturrock
R D
RD
D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D004880:Ergothioneine / Q000378:metabolism*; D004912:Erythrocytes / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D010100:Oxygen / Q000502:physiology*
D004880:Ergothioneine; D010100:Oxygen