title: [The protoplasts of Acremonium chrysogenum. Biochemical and morphological studies].
abstract: A procedure for preparing stable A. chrysogenum protoplasts capable of 60 per cent regeneration was developed. Two morphogenetic types of the regeneration were detected. The variants isolated after the protoplast regeneration were characterized by wide ranges of morphological variation. Capacity for the antibiotic production varied from 60 to 160 per cent of the activity of the starting strain. A procedure for isolating functionally active mitochondria from protoplasts of A. chrysogenum was also developed. Their main bioenergetic parameters were studied. In the respiratory chain of the A. chrysogenum mitochondria there were detected three conjugation sites of oxidative phosphorylation.
authors: G N
authors: GN
authors: Telesnina
authors: Iu O
authors: IuO
authors: Sazykin
authors: R A
authors: RA
authors: Zviagil'skaia
authors: S V
authors: SV
authors: Dmitrieva
authors: I N
authors: IN
authors: Krakhmaleva
mesh_terms: D000164:Acremonium / Q000648:ultrastructure*; D000362:Agar; D002511:Cephalosporins / Q000096:biosynthesis; D003470:Culture Media; D004734:Energy Metabolism; D008928:Mitochondria / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D002511:Cephalosporins; D003470:Culture Media; C025163:cephalosporin C; D000362:Agar
vernacular_title: Protoplasty Acremonium chrysogenum. Biokhimicheskie i morfologicheskie issledovaniia.