Monoclonal antibodies specific for non-glycosylated porcine prolactin and for pituitary porcine prolactin.
Glycosylated and non-glycosylated forms of porcine pituitary prolactin were prepared using Concanavalin A Sepharose chromatography. Anti-prolactin monoclonal antibodies were screened for their ability to distinguish these two forms. One monoclonal antibody (17D9) exhibited high affinity binding for the non-glycosylated form of porcine prolactin, but little or no affinity for the glycosylated form. Using this antibody in conjunction with other monoclonals which equally recognize both forms, we developed immunoassays which can be used to determine the amount of the glycosylated vs. non-glycosylated prolactin in serum or other tissue samples.
Scott
M G
MG
Mallinckrodt, Inc., Diagnostic Products Division, St. Louis, MO 63134.
Lin
W H
WH
Lyle
L R
LR
Atkinson
P R
PR
Seely
J E
JE
Markoff
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D006031:Glycosylation; D051379:Mice; D008807:Mice, Inbred BALB C; D010902:Pituitary Gland / Q000032:analysis*; D011388:Prolactin / Q000276:immunology*; D011869:Radioligand Assay; D013552:Swine
D000911:Antibodies, Monoclonal; D011388:Prolactin