Effects of an aplastic anemia urinary extract on mouse erythroid progenitor cells in vivo.
We investigated the in vivo effects of a crude extract from the urine of aplastic anemia patients (AA urinary extract) on erythroid precursor cells in the femoral bone marrow and spleens of normal adult mice. A single intraperitoneal injection of AA urinary extract induced a significant increase in the number of splenic erythroid burst-forming units (BFU-e) and erythroid colony-forming units (CFU-e) within 24 h after injection. We then injected pure recombinant erythropoietin (Epo) equivalent to the amount present in the urinary extract. This addition increased the number of splenic CFU-e by almost the same degree as the amount induced by the AA urinary extract 24 h after injection, but failed to elicit any change in the number of splenic BFU-e. In other studies, mice were injected with the same amount of lipopolysaccharide (LPS) and/or pure Epo as that present in the AA urinary extract. Experiments with Limulus amebocyte lysate-adsorbed (endotoxin-depleted) or nonadsorbed (endotoxin-containing) AA urinary extracts showed that endotoxin contamination interfered with the increase in numbers of marrow CFU-e and enhanced the increase in splenic CFU-e numbers induced by pure Epo or Epo activity in the AA urinary extract. The number of splenic BFU-e, however, was not affected by administration of LPS and/or Epo or by adsorbed endotoxin. These data suggest that AA urinary extract contains a stimulating activity for mouse splenic BFU-e, and that this activity is not attributable to the Epo activity or endotoxin contamination within the urinary extract.
Kamamoto
T
T
Hipple Cancer Research Center, Dayton, Ohio 45439-2092.
Kuriya
S
S
Murphy
M J
MJ
D000327:Adsorption; D000741:Anemia, Aplastic / Q000652:urine*; D000818:Animals; D003114:Colony-Forming Units Assay; D004731:Endotoxins / Q000502:physiology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D004921:Erythropoietin / Q000008:administration & dosage* / Q000494:pharmacology / Q000502:physiology; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D006737:Horseshoe Crabs; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology
D004731:Endotoxins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D004921:Erythropoietin