A kidney perfusion method for metabolism studies with chickens using propachlor as a model.
1. A procedure for directly perfusing chicken kidneys in situ with xenobiotics is described. 2. When propachlor (2-chloro-N-isopropylacetanilide) or the glutathione, cysteine and mercapturic acid conjugates of propachlor were individually perfused through chicken kidneys, propachlor mercapturate was isolated from the urine and identified as an end product, indicating that all enzymes necessary for mercapturic acid formation were present in chicken kidney.
Davison
K L
KL
Metabolism and Radiation Research Laboratory, U.S. Department of Agriculture, Fargo, ND 58105.
Bakke
J E
JE
Larsen
G L
GL
D000083:Acetanilides / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D006041:Goats; D007668:Kidney / Q000201:enzymology / Q000378:metabolism*; D008954:Models, Biological; D010477:Perfusion; D051381:Rats; D012756:Sheep; D013045:Species Specificity
D000083:Acetanilides; C011088:propachlor