6-Nitrocholesterol inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase and tumor cell growth.
6-Nitrocholesterol has been shown to cause a 50% reduction in the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in animal cells in culture at 1.9 microM and it has relative binding affinity for the cytosolic oxysterol binding protein of 357 nM in cell-free extracts from the same cell line. In addition, significant cytotoxicity was observed when this sterol was incubated with hepatoma and lymphoma cells in culture.
Parish
E J
EJ
Department of Chemistry, Auburn University, Alabama 36849.
Dixon
K L
KL
Chitrakorn
S
S
Taylor
F R
FR
Luu
B
B
Ourisson
G
G
D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D051379:Mice; D051381:Rats; D011987:Receptors, Steroid / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000098825:Oxysterol Binding Proteins
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins; C056245:6-nitrocholesterol; D002784:Cholesterol