A tissue culture assay for tetrodotoxin, saxitoxin and related toxins.
In the presence of ouabain, veratridine enhances sodium influx in the mouse neuroblastoma cell line Neuro-2A (ATCC, CCL131), causing cellular swelling and subsequent death. Tetrodotoxin (puffer fish toxin) or saxitoxin (paralytic shellfish poison), both of which block the sodium channel of excitable membranes, antagonize this effect, enabling cell growth to continue. This phenomenon was used as the basis of a new assay for these toxins. It is also possible to estimate the quantity of TTX from the relationship between TTX concentration and percentage of living cells. This new method is simple, inexpensive, and sensitive, and may replace the conventional mouse bioassay.
Kogure
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Center of Marine Biotechnology, University of Maryland, Baltimore 21202.
Tamplin
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Simidu
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U
Colwell
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D000818:Animals; D001681:Biological Assay / Q000379:methods; D002460:Cell Line; D008387:Marine Toxins / Q000032:analysis* / Q000494:pharmacology; D051379:Mice; D010042:Ouabain / Q000494:pharmacology; D012530:Saxitoxin / Q000032:analysis* / Q000494:pharmacology; D013779:Tetrodotoxin / Q000032:analysis* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects; D014701:Veratridine / Q000494:pharmacology
D008387:Marine Toxins; D012530:Saxitoxin; D013779:Tetrodotoxin; D010042:Ouabain; D014701:Veratridine