In vitro cultivation of a Babesia isolated from a white-tailed deer (Odocoileus virginianus).
Pyriforms and ring forms of Babesia odocoilei were detected in thin blood smears obtained from a white-tailed deer killed by a hunter in Anderson County, Texas. Erythrocytes from the deer were cultured and the parasites maintained through 8 serial subcultures during 1 mo. The parasite was successfully established in culture using Medium 199 supplemented with either 20% deer serum or 40% normal adult bovine serum. The highest parasitemia observed was 30% and more than 4 parasites per erythrocyte were often observed. Cultured B. odocoilei remained infective for a susceptible white-tailed deer.
Holman
P J
PJ
Department of Veterinary Microbiology and Parasitology, Texas A&M University, College Station 77843.
Waldrup
K A
KA
Wagner
G G
GG
D000818:Animals; D001403:Babesia / Q000254:growth & development* / Q000472:pathogenicity; D001404:Babesiosis / Q000469:parasitology*; D003470:Culture Media; D003670:Deer / Q000469:parasitology*; D005260:Female; D005615:Freezing; D008297:Male; D011309:Preservation, Biological
D003470:Culture Media