Nucleotide sequence of a cDNA clone encoding mouse transition protein 1.
We have determined the nucleotide sequence of cDNA clones encoding mouse transition protein 1 (TP1), a basic nuclear protein involved in nuclear condensation during spermiogenesis. The nucleotide sequence predicts that transition protein 1 in rats and mice differs by only one amino acid. The rate of substitution of nucleotides in the coding region of mouse and rat transition protein 1 mRNA is close to the average of many proteins in rats and mice, and the usage of degenerate codons is typical of the mouse. The identification of this cDNA clone, in conjunction with previous work (Kleene et al. (1983) Dev. Biol. 98, 455-464; Hecht et al. (1986) Exp. Cell Res. 164, 183-190), demonstrates that the mRNA for mouse transition protein 1 accumulates during the haploid phase of spermatogenesis.
Kleene
K C
KC
Department of Biology, University of Massachusetts, Boston 02125.
Borzorgzadeh
A
A
Flynn
J F
JF
Yelick
P C
PC
Hecht
N B
NB
D000818:Animals; D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D008297:Male; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013091:Spermatogenesis
D002868:Chromosomal Proteins, Non-Histone; D003062:Codon; D009687:Nuclear Proteins; C053886:spermatid transition proteins