title: Comparative study on the main membrane-surface sialoglycopeptides released from young and old human erythrocytes with trypsin.
abstract: 1. The amount of sialoglycopeptide (SGP) mixture released from young (Y) erythrocytes by trypsin treatment was significantly larger than that from old (O) ones. 2. The two main membrane-surface sialoglycopeptides (Y-SGP and O-SGP) were isolated in yields of about 45% and 42% from the mixtures by a combination of Sephadex G-50 superfine gel filtration with DEAE-cellulose chromatography, respectively. Molecular weights of 12,000 and 12,100, respectively, were found for the Y-SGP and the O-SGP. 3. The Y-SGP showed a greater proportion of sialic acid than the O-SGP. The contents of galactose, N-acetylglucosamine and N-acetylgalactosamine of the Y-SGP were significantly lower than those of the O-SGP. 4. Both the SGPs exhibited the same reactivities against the MN reagents, but the reactivities against the lectins of Limulus polyphemus and Phaseolus vulgaris (E) were stronger in the Y-SGP than in the O-SGP. Neither the Y-SGP nor the O-SGP showed the reactivity to Arachis hypogaea (anti-T) lectin.
authors: Department of Legal Medicine, School of Medicine, Keio University, Japan.
authors: T
authors: T
authors: Shinozuka
authors: S
authors: S
authors: Takei
authors: J
authors: J
authors: Yanagida
authors: H
authors: H
authors: Watanabe
authors: S
authors: S
authors: Ohkuma
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000032:analysis*; D006801:Humans; D012795:Sialoglycoproteins / Q000097:blood*; D014357:Trypsin / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D012795:Sialoglycoproteins; D014357:Trypsin