Reactivation of creatine kinase activity by preincubation with buffered NAC diluent.
We have shown that several commercial control sera containing reversibly inactivated creatine kinase are reactivated by pre-incubation in N-acetyl cysteine assay diluent. The increase in activity can proceed for up to 4 h. This reactivation is not seen in patients' sera even after storage for 14 days at -20 degrees C, 7 days at 4 degrees C, or 2 days at 20 degrees C, during which time no loss of activity was evident. This study brings into question the validity of using consensus values to standardise enzyme assays.
Penny
A F
AF
Biochemistry Department, Seacroft Hospital, Leeds, UK.
Jennings
A
A
D000111:Acetylcysteine / Q000032:analysis*; D002021:Buffers; D003402:Creatine Kinase / Q000032:analysis*; D004793:Enzyme Reactivators / Q000032:analysis*; D006801:Humans; D013696:Temperature
D002021:Buffers; D004793:Enzyme Reactivators; D003402:Creatine Kinase; D000111:Acetylcysteine