Oltipraz-induced reduction in schistosomal glucose utilization rates.
The rate of phosphorylation of 2-deoxyglucose (2DG) was determined by sequential pulsing of schistosomes (male and female Schistosoma mansoni) with [3H- and 14C]-2-deoxy-D-glucose. The relative phosphorylation rate of 2-[3H]-2DG to 1-[14C]-D-glucose (i.e. the phosphorylation coefficient) was also measured in male and female schistosomes. Even though 2DG is taken up more rapidly than glucose, it is phosphorylated at a much slower rate in S. mansoni. Mated schistosomes phosphorylate 2DG and glucose at a greater rate than do unmated worms. In contrast, the phosphorylation coefficient is greater in separated than mated schistosomes. In schistosomes exposed to oltipraz for short time periods (6 min, at a concentration of 10 micrograms/ml) glucose utilization rates were significantly reduced in (both mated and separated) female S. mansoni and by a similar magnitude (not significant) in males.
Cornford
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EM
Southwest-Regional VA Epilepsy Center, Veterans Administration Wadsworth Medical Center, Los Angeles, California 90073.
D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D011719:Pyrazines / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000378:metabolism; D012556:Schistosomicides / Q000494:pharmacology*; D013871:Thiones; D013876:Thiophenes
D011719:Pyrazines; D012556:Schistosomicides; D013871:Thiones; D013876:Thiophenes; C026209:oltipraz; D003847:Deoxyglucose; D005947:Glucose