title: Heat sensitivity, thermotolerance, and profile of protein synthesis of human bone marrow progenitors.
abstract: Hyperthermic sensitivity, kinetics of thermotolerance induction and decay, and profile of heat shock protein synthesis were studied in human granulocyte-macrophage progenitors and nucleated bone marrow cells, respectively. The D0 of the heat survival curves of human granulocyte-macrophage colony-forming cells at 43 degrees C, 44 degrees C, and 45 degrees C were 23, 12, and 5 min, respectively. The kinetics of thermotolerance induction was measured with a triggering dose of 44 degrees C/20 min, 45 degrees C/10 min, 41 degrees C/2 h, and 42 degrees C/1 h and 4 h. The cells were then challenged with 44 degrees C or 45 degrees C after incubation for 2, 4, 6, 24, and 48 h at 37 degrees C. In all cases, except for 41 degrees C/2 h, thermotolerance was maximum at 2 to 6 h, began to decay at 24 h, and decayed completely by 48 h. At the 41 degrees C/2-h triggering dose, the thermotolerance decayed completely by 24 h. The heat shock protein synthesis was measured after a triggering dose of 44 degrees C/20 min or 45 degrees C/10 min. Synthesis of Mr 70,000 and 87,000 heat shock proteins in the total nucleated bone marrow cells was evident at least for 8 h after the initial heating.
authors: Department of Radiation Research, City of Hope National Medical Center, Duarte, California 91010.
authors: N F
authors: NF
authors: Mivechi
mesh_terms: D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002470:Cell Survival; D002478:Cells, Cultured; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D014176:Protein Biosynthesis
chemical_list: D006360:Heat-Shock Proteins