KDN-glycoprotein: a novel deaminated neuraminic acid-rich glycoprotein isolated from vitelline envelope of rainbow trout eggs.
A new acidic glycoprotein containing deaminated neuraminic acid (KDN = 3-deoxy-D-glycero-D-galacto-nonulosonic acid; greater than 50%, w/w) was isolated from vitelline envelope of the unfertilized eggs of rainbow trout (Salmo gairdneri). This glycoprotein is designated as "KDN-glycoprotein" because it contains only KDN but no sialic acid as the acidic carbohydrate moieties. Other major carbohydrate components of KDN-glycoprotein were Gal and GalNAc. Thr and Ala accounted for 71% (mol/mol) of amino acid composition. A possible occurrence of KDN-KDN linkages, i.e. oligoKDN groups has been suggested in the carbohydrate chains presumably linked O-glycosidically to the core protein.
Inoue
S
S
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Kanamori
A
A
Kitajima
K
K
Inoue
Y
Y
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D013400:Sugar Acids / Q000032:analysis*; D014337:Trout; D014817:Vitelline Membrane / Q000032:analysis*
D000596:Amino Acids; D006023:Glycoproteins; D013400:Sugar Acids; C050008:3-deoxyglycero-galacto-nonulosonic acid