The effect of L-carnitine on the apolipoprotein pattern of rats fed a cholesterol-rich diet.
1. L-Carnitine was administered to hypercholesterolemic rats for eight days, after which the blood was collected by intracardiac puncture and lipoproteins were collected by preparative ultracentrifuge. 2. The chemical composition of lipoproteins of normal rats and hypercholesterolemic rats before and after L-carnitine treatment was determined. 3. The total serum B and E apoproteins were assayed by rocket electrophoresis, while apoproteins in VLDL, IDL, LDL and HDL were determined by densitometric scanning of SDS and TMU polyacrylamide disc gel electrophoresis. 4. The results obtained showed that the L-carnitine treatment tends to restore to normal values both the chemical composition of lipoproteins and the apoproteins pattern of rats fed on a diet enriched with cholesterol.
Mondola
P
P
Institute of Physiology, Medical Faculty, Naples University, Italy.
Belfiore
A
A
Santangelo
F
F
Santillo
M
M
D000818:Animals; D001053:Apolipoproteins / Q000097:blood*; D001055:Apolipoproteins B / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood; D002331:Carnitine / Q000494:pharmacology*; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D007123:Immunoelectrophoresis, Two-Dimensional; D008074:Lipoproteins / Q000097:blood; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001053:Apolipoproteins; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D002791:Cholesterol, Dietary; D008074:Lipoproteins; D002331:Carnitine