The three-dimensional structures of S-layers of two novel Eubacterium species isolated from inflammatory human processes.
The three-dimensional structures of the crystalline surface layers of two species of Eubacteria have been determined by electron microscopy and computerized image processing. The S-layer of Eubacterium sp. ES4C has tetragonal symmetry, with a unit cell spacing of 10.6 nm and a thickness of 9.5 nm, while that of Eubacterium sp. AHN 990 has hexagonal symmetry a = b = 15.7 nm and a thickness of 13 nm. The resolutions in the reconstructions were 2.5 nm and 1.8 nm, respectively. The reconstruction of the S-layer of strain ES4C reveals a distinct domain structure: a major tetramer, arms connecting adjacent unit cells, and a minor tetramer. The S-layer of strain AHN 990, on the other hand, has a rather complex arrangement, centred around the six-fold axis.
Sjögren
A
A
Department of Structural Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
Wang
D N
DN
Hovmöller
S
S
Haapasalo
M
M
Ranta
H
H
Kerosuo
E
E
Jousimies-Somer
H
H
Lounatmaa
K
K
D005051:Eubacterium / Q000302:isolation & purification / Q000648:ultrastructure*; D005613:Freeze Etching; D006801:Humans; D007249:Inflammation / Q000382:microbiology; D008854:Microscopy, Electron; D008961:Models, Structural