Similarity of the sugar moiety of human alkaline phosphatases between the kidney cortex and duodenum, or medulla and ileum.
Characteristics of human renal cortex and medulla alkaline phosphatase (ALP) were compared. Enzymatic and hydrophobic properties of both ALPs were almost similar. However, the results of concanavalin A affinity chromatography and wheat germ agglutinin affinity electrophoresis, exhibited that sugar chain structure(s) might be different between the cortex and medulla ALPs. In addition, the molecular mass and substrate specificity differed from each other, and these results of cortex and medulla ALPs were well accordant with those of human duodenal and ileal ALPs, respectively, as described previously (Clin Chim Acta 1987;163:279-287).
Koyama
I
I
Department of Biochemistry, Saitama Medical School, Tokyo, Japan.
Arai
Y
Y
Miura
M
M
Matsuzaki
H
H
Sakagishi
Y
Y
Komoda
T
T
D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002846:Chromatography, Affinity; D004386:Duodenum / Q000201:enzymology*; D004586:Electrophoresis; D006801:Humans; D007082:Ileum / Q000201:enzymology*; D007672:Kidney Cortex / Q000201:enzymology*; D007679:Kidney Medulla / Q000201:enzymology*
D002241:Carbohydrates; D000469:Alkaline Phosphatase