Hepatic cysteine dioxygenase activity and sulfur amino acid metabolism in rats: possible indicators in the evaluation of protein quality.
Experiments were carried out to examine the possibility that the sulfur amino acid metabolism of rats may be an indicator of the nutritional value of dietary protein. Rats were fed diets containing 8, 16 or 24% of gluten, soy protein or casein for 3 wk. Hepatic cysteine dioxygenase activity, hepatic concentration of glutathione, cysteine and taurine and urinary taurine were examined. In addition, the sulfur amino acid metabolism of rats fed these diets fortified with the appropriate first limiting amino acid for 7 d was also examined. High urinary taurine excretion was observed in the three gluten groups, whereas very low urinary taurine excretion was observed with up to 24% soy protein or up to 16% casein. The hepatic hepatic cysteine dioxygenase activities of the gluten diet groups were higher than those of corresponding soy protein or casein diet groups, except that of rats fed the 24% casein diet. The hepatic concentrations of both glutathione and cysteine in gluten diet groups were also higher than those of corresponding soy protein or casein diet groups, except 24% soy protein and 16 and 24% casein diet groups. In rats fed the casein or soy protein diets urinary taurine excretion and hepatic cysteine dioxygenase activity increased with increasing methionine supplementation, the first limiting amino acid. Conversely, in rats fed the gluten diet both urinary taurine excretion and hepatic cysteine dioxygenase activity decreased with increasing lysine supplementation, the first limiting amino acid. These findings suggest that urinary taurine excretion and hepatic cysteine dioxygenase activity may be useful as sensitive indicators of the nutritional value of dietary protein.
Hosokawa
Y
Y
Division of Maternal and Child Nutrition, National Institute of Nutrition, Tokyo, Japan.
Niizeki
S
S
Tojo
H
H
Sato
I
I
Yamaguchi
K
K
D000603:Amino Acids, Sulfur / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D003545:Cysteine / Q000378:metabolism; D050558:Cysteine Dioxygenase; D004044:Dietary Proteins / Q000378:metabolism*; D049308:Dioxygenases; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000378:metabolism; D009753:Nutritive Value; D010105:Oxygenases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013654:Taurine / Q000652:urine
D000603:Amino Acids, Sulfur; D004044:Dietary Proteins; D013654:Taurine; D008715:Methionine; D010105:Oxygenases; D049308:Dioxygenases; D050558:Cysteine Dioxygenase; D003545:Cysteine