Photodiode array detection of peptide-nucleoside antibiotics.
The discrimination within fermentation matrices of some structurally related peptide-nucleosides is vastly simplified by coupling photodiode array detection to high-performance liquid chromatography (HPLC). Blasticidin S analogues and other closely related nucleosides can easily be discriminated with on-line photodiode array spectral acquisition (PDA). From the combined HPLC-PDA evaluation of the fermentation filtrate from culture broth 83-2245, two blasticidin S type compounds, Sch 36605 and Sch 36606 could be detected. Mildiomycin, a structurally related nucleoside produced from ATCC culture 31120, could be detected during preliminary isolation without an authentic reference for comparison. Fast atom bombardment mass spectroscopic evaluations and mass-analysed ion kinetic energy spectra of these nucleosides confirmed their respective identities.
Mierzwa
R
R
Pharmaceutical Research Division, Schering-Plough Corporation, Bloomfield, NJ 07003.
Cooper
R
R
Pramanik
B
B
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D005285:Fermentation; D013058:Mass Spectrometry; D009705:Nucleosides / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D010777:Photochemistry; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D009705:Nucleosides; D010455:Peptides; C004500:blasticidin S; D003596:Cytosine; C017539:mildiomycin