title: Expression of cartilage-matrix genes and localization of their translation products in the embryonic chick eye.
abstract: The presence of cartilage-matrix molecules in the embryonic chick eye was shown by means of measuring RNA expression and immunostaining studies. The molecules examined were proteoglycan core protein (PG-core), link protein (LP), cartilage-matrix protein (CMP) and type II collagen. The genes encoding these proteins are expressed in 8- to 11-day embryonic chick eyes at constant steady-state levels. Immunopositive stained tissues include sclera, choroid, cornea, lens capsule (PG-core, LP, CMP), lens epithelium, lens fibers (LP, CMP) and the membranes in the retina (LP). In addition, data from comparative studies employing the eyes from the proteoglycan core-protein-deficient mutant, nanomelia, indicate that levels of mRNA for core protein are almost absent and that CMP is reduced in the above-mentioned tissues.
authors: La Jolla Cancer Research Foundation, Cancer Research Center, CA 92037.
authors: P A
authors: PA
authors: Tsonis
authors: P F
authors: PF
authors: Goetinck
mesh_terms: D053674:Aggrecans; D000818:Animals; D002356:Cartilage / Q000032:analysis*; D002642:Chick Embryo; D004587:Electrophoresis, Agar Gel; D005109:Extracellular Matrix / Q000032:analysis*; D016326:Extracellular Matrix Proteins; D005136:Eye Proteins / Q000032:analysis*; D005796:Genes; D006023:Glycoproteins / Q000032:analysis; D037181:Lectins, C-Type; D009154:Mutation; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D012333:RNA, Messenger; D000099249:Proteoglycan Link Protein
chemical_list: D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D005136:Eye Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D011506:Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D000099249:Proteoglycan Link Protein