High-performance liquid chromatographic method for the determination of alpha-amanitin and phalloidin in human plasma using the column-switching technique and its application in suspected cases of poisoning by the green species of amanita mushroom (Amanita phalloides).
A reversed-phase high-performance liquid chromatographic assay has been developed for the simultaneous determination of alpha-amanitin and phalloidin in human plasma. The procedure is based on the enrichment of the toxins on a pre-column, followed by the transfer of both compounds in a foreflush mode to the analytical column. alpha-Amanitin and phalloidin can be quantified reliably down to a minimum concentration of 10 ng/ml in plasma (relative standard deviation less than 10%). An alternative method is recommended for hepatic coma patients.
Rieck
W
W
Institut für Gerontologie, Universität Erlangen-Nürnberg, F.R.G.
Platt
D
D
D000363:Agaricales; D000545:Amanita; D000546:Amanitins / Q000097:blood*; D002648:Child; D006801:Humans; D007202:Indicators and Reagents; D009145:Mushroom Poisoning / Q000097:blood*; D009842:Oligopeptides / Q000097:blood*; D010590:Phalloidine / Q000097:blood*; D012997:Solvents
D000546:Amanitins; D007202:Indicators and Reagents; D009842:Oligopeptides; D012997:Solvents; D010590:Phalloidine