title: 14C-labeling of synthetic peptides.
abstract: Two methods are described for the labeling of synthetic peptides using iodo[14C]acetic acid. The first procedure may be employed when the synthetic fragment contains a cysteine with a free sulfhydryl group. Alternatively, a commercial amino-protected cysteine may be carboxymethylated using radioactive iodoacetic acid. This derivative can be added to the growing peptide chain in the manual or automatic solid-phase synthesis of the fragment.
authors: Istituto Regina Elena, Laboratory of Biochemistry, Rome, Italy.
authors: A
authors: A
authors: Chersi
authors: M L
authors: ML
authors: Trinca
authors: M
authors: M
authors: Camera
mesh_terms: D000595:Amino Acid Sequence; D002233:Carbocysteine / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D011869:Radioligand Assay
chemical_list: D002250:Carbon Radioisotopes; D005136:Eye Proteins; D007202:Indicators and Reagents; D010446:Peptide Fragments; D010455:Peptides; C052615:peptide M, retinal S antigen; D002233:Carbocysteine