title: Electron microscopic autoradiography of D-[3H]aspartate uptake sites in mouse cerebellar slices shows poor labelling of mossy fibre terminals.
abstract: Recent evidence indicates that mossy fibres in the cerebellum may use glutamate as their transmitter. To determine if mossy fibre terminals accordingly express a high-affinity uptake mechanism for excitatory amino acids, mouse cerebellar slices were incubated for 3 h with 1 microM D-[3H]aspartate and examined by electron microscopic autoradiography. In the granule cell layer, granule cell bodies were clearly labelled but the majority of mossy fibre terminals were without silver grains.
authors: Department of Veterinary Physiology and Pharmacology, University of Liverpool, U.K.
authors: G
authors: G
authors: Garthwaite
authors: J
authors: J
authors: Garthwaite
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D001345:Autoradiography; D002531:Cerebellum / Q000378:metabolism* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D014316:Tritium
chemical_list: D014316:Tritium; D001224:Aspartic Acid