A cytopreparatory method for cerebrospinal fluid in which the cell yield is high and the fluid is saved for chemical analysis.
A method for the concentration of cells from cerebrospinal fluid is described. An adaptation of a commercial cytochamber, consisting of a holder that fixes a disposable chamber directly on a microscope slide, was used. The cells were spun down in a conventional swing-out centrifuge, which was provided with a bucket for the cytochamber system. After removing most of the supernatant with a pipette, the remaining fluid was absorbed by means of a suction device consisting of a disposable pipette tip covered with a piece of Leukopor and filled with Sephadex G10 beads. The method gives a high recovery of cells (90%), together with a good preservation of cell morphology, and leaves about 80% of the fluid available for analysis of the soluble components.
Tutuarima
J A
JA
Department of Clinical Chemistry, Academic Medical Center, Amsterdam, The Netherlands.
Hische
E A
EA
Sylva-Steenland
R M
RM
van der Helm
H J
HJ
D002498:Centrifugation; D002555:Cerebrospinal Fluid / Q000032:analysis / Q000166:cytology*; D006801:Humans; D007958:Leukocyte Count