[Surface charge of erythrocyte membrane during disorders of lipid metabolism from the data of microelectrophoresis, H+-titration and fluorescence studies].
The surface charges and the isoelectric points (pI) as well as changes in these values depending on the lipid composition are determined for erythrocyte membranes of rat, rabbit, bull and man under conditions of hypercholesterinemia, atherosclerosis, D-hypovitaminosis and in experiments in vitro using the techniques of microelectrophoresis and positive charged ligands (proton, probe astraphloxin) binding. The ionogenic groups forming charge and essential differences in their nature (pK) and quantity depending on the erythrocyte type are found. Inverse correlation between the content of cholesterol and cholesterol ethers and the value of erythrocyte membrane surface charges was established for each sample examined. A comparative evaluation of the methods used was conducted.
Bondar'
O P
OP
Kholodova
Iu D
IuD
Smirnova
I P
IP
Voziian
P A
PA
D000818:Animals; D001161:Arteriosclerosis / Q000097:blood; D002417:Cattle; D004036:Diet, Atherogenic; D004586:Electrophoresis; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005456:Fluorescent Dyes; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D007525:Isoelectric Focusing; D008563:Membrane Lipids / Q000032:analysis / Q000097:blood*; D008564:Membrane Potentials; D011817:Rabbits; D051381:Rats; D014808:Vitamin D Deficiency / Q000097:blood
D005456:Fluorescent Dyes; D008563:Membrane Lipids
Poverkhnostnyĭ zariad membran éritrotsitov pri narusheniiakh lipidnogo obmena po dannym mikroélektroforeza, H+-titrovaniia i fluorestsentnykh issledovaniĭ.