title: Betaine aldehyde dehydrogenase polymorphism in spinach: genetic and biochemical characterization.
abstract: Spinach (Spinacia oleracea L.) has a major chloroplastic isozyme of betaine aldehyde dehydrogenase (BADH) and a minor cytosolic one. Among a diverse collection of spinach accessions, three electrophoretic banding patterns of chloroplastic BADH were found: two were single banded and one was triple banded. Genetic analysis of these patterns indicated that chloroplastic BADH is encoded by a single, nuclear gene with two alleles, designated slow (S) and fast (F), and that products of these alleles can hybridize to form either homodimers or a heterodimer. The S allele was by far the most common among the accessions examined. Native and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the SS and FF homodimers differ in charge but not molecular weight.
authors: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824.
authors: E A
authors: EA
authors: Weretilnyk
authors: A D
authors: AD
authors: Hanson
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000483:Alleles; D050728:Betaine-Aldehyde Dehydrogenase; D003600:Cytosol / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics*; D008970:Molecular Weight; D010641:Phenotype; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D007527:Isoenzymes; D000445:Aldehyde Oxidoreductases; D050728:Betaine-Aldehyde Dehydrogenase