Characterization and macromolecular association of proteoglycans produced by pig arterial smooth muscle cells in culture.
1. Medium and cell-layer proteoglycans from pig aorta smooth muscle cells in culture were compared. In both compartments, the main proteoglycans contained chondroitin sulfate-dermatan sulfate chains of 40 kDalton. 2. However, cell-layer proteoglycans differed from those of the medium by the presence of: (a) some small-size proteoglycans; (b) a greater amount of heparan sulfate; (c) chondroitin sulfate-dermatan sulfate enriched in iduronate and in 4 sulfate- (instead of 6 sulfate-) residues. 3. During dissociation-reassociation assays of arterial proteoglycans with exogenous hyaluronate or "aggregate" proteoglycans, the in vitro formation of complexes appeared to involve inter-associations between proteoglycans molecules, in addition to aggregation with hyaluronate.
Deudon
E
E
Laboratoire de Biochimie, INSERM U.181, Faculté de Médecine Saint-Antoine, Paris, France.
Brahimi-Horn
M C
MC
Breton
M
M
Berrou
E
E
Picard
J
J
D000818:Animals; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D006025:Glycosaminoglycans / Q000032:analysis; D006820:Hyaluronic Acid / Q000494:pharmacology; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis*; D013552:Swine
D006025:Glycosaminoglycans; D011509:Proteoglycans; D006820:Hyaluronic Acid