Interaction of type I collagen with phosphatidylcholine vesicles.
Type I collagen interacts with phosphatidylcholine vesicles. This conclusion has been obtained after ultracentrifugation, fluorescence polarization, circular dichroism and differential scanning calorimetry studies. The protein conformation is not modified by the presence of phospholipids. Collagen modifies both the enthalpy change and cooperativity of the phosphatidylcholine phase transition. All these effects exhibit a saturating behavior. The obtained results are interpreted in terms of a peripheral interaction between collagen and the phospholipid vesicles.
Martinez del Pozo
A
A
Departamento de Bioquimica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
Oñaderra
M
M
Laynez
J
J
Gavilanes
J G
JG
D000818:Animals; D002151:Calorimetry; D002942:Circular Dichroism; D003094:Collagen / Q000032:analysis*; D010713:Phosphatidylcholines / Q000032:analysis*; D011487:Protein Conformation; D051381:Rats; D013050:Spectrometry, Fluorescence; D014461:Ultracentrifugation; D014783:Viscosity
D010713:Phosphatidylcholines; D003094:Collagen