Protein kinase C activity in soluble fractions from glial cells in primary culture and subcultures.
Protein kinase C (calcium + phospholipid-dependent kinase) activity has been measured in soluble 100,000 g fractions from mixed glial cells in primary culture; in 12 day cultures the specific activity (mean +/- S.D.) was 184 +/- 10 pmol 32P incorporated/10 min/mg protein. In glial cell subcultures lacking protoplasmic astrocytes protein kinase C specific activity was lower. An inhibitor of protein kinase C in 100,000 g supernatants was removed by chromatography through DE-52 anion exchange resin increasing the specific activity of the calcium + phospholipid-dependent kinase about 20 times. Protein kinase C was also associated with membrane fractions from glial cells; the membrane-associated enzyme had a higher specific activity than in the cytoplasm.
Murphy
J A
JA
Department of Biology, University of York, U.K.
Chapman
J A
JA
Suckling
A J
AJ
Rumsby
M G
MG
D000818:Animals; D001253:Astrocytes / Q000201:enzymology / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D009457:Neuroglia / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
D011493:Protein Kinase C; D002118:Calcium