title: Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.
abstract: We have analyzed the coding capacity of B19 parvovirus transcripts by in vitro translation using the negative hybrid selection technique. Five different antisense oligonucleotides (18-mers) corresponding to different portions of the B19 genome were hybridized to RNA samples extracted from human erythroid bone marrow cells infected with B19 parvovirus in vitro, and RNase H was added to cleave specific B19 RNA molecules at selected sites. B19-specific translation products of these RNA samples were determined by immunoprecipitation. We localized the B19 nonstructural protein to the left-side transcript and the two capsid proteins to overlapping transcripts from the right side of the genome.
authors: Cell Biology Section, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.
authors: K
authors: K
authors: Ozawa
authors: J
authors: J
authors: Ayub
authors: N
authors: N
authors: Young
mesh_terms: D004900:Erythroblasts / Q000378:metabolism; D005814:Genes, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D010321:Parvoviridae / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics
chemical_list: D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D014764:Viral Proteins