title: Amino acid sequence of porcine heart fumarase.
abstract: The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.
authors: Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110.
authors: J C
authors: JC
authors: Sacchettini
authors: M W
authors: MW
authors: Frazier
authors: D C
authors: DC
authors: Chiara
authors: L J
authors: LJ
authors: Banaszak
authors: G A
authors: GA
authors: Grant
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005649:Fumarate Hydratase / Q000032:analysis*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D010449:Peptide Mapping; D011487:Protein Conformation; D013552:Swine
chemical_list: D005649:Fumarate Hydratase