Separation of two different populations of granulocytes of Nereis diversicolor (Annelida) by selective agglutination with lectins.
Methods for obtaining coelomocyte populations from Nereis diversicolor by selective agglutination have been developed. Two main types of granulocytes have been successfully separated and recovered using respectively galactose and fucose specific lectins. 85 to 90% of the separated cells were viable.
M'Beri
M
M
Laboratoire de Biologie Animale et UA 148 CNRS, Université de Lille Flandres Artois, Villeneuve d'Ascq.
Debray
H
H
Dhainaut
A
A
D000818:Animals; D000845:Annelida / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D005643:Fucose; D005690:Galactose; D006098:Granulocytes / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology*
D037102:Lectins; D005643:Fucose; D005690:Galactose