[Interaction of tyrosine-phenol-lyase from Citrobacter intermedius with amino acids and their derivatives: factors determining the effectiveness of binding].
The inhibition by L-amino acids and their derivatives of tyrosine phenol-lyase is investigated. Tyramine, alpha-phenylethylamine and tryptamine have no detectable inhibition effect and hence are weakly bonded by an active site. The aromatic amino acid amides are competitive inhibitors but do not manifest an enzymatic isotope exchange of alpha-proton in D2O. Free amino acids however are competitive inhibitors and in the majority of cases exchange alpha-proton. The presence of COOH-group is therefore an important feature which determines the binding efficiency and causes the "active" conformation of the amino acid-PLP complex labelising alpha-proton. In the absence of functional and bulky groups in the amino acid side chain the hydrophobicity is found to be the main factor determining the binding efficiency. For these amino acids a correlation exists between-RTlnKi and side chain hydrophobicity. The amino acids bearing the bulky groups, i. e. valine, leucine and isoleucine have reduced binding efficiency. Lysine and arginine bearing positively charged functional groups possess no inhibition effect. Aspartic and glutamic acids are anomalously strong inhibitors taking into consideration low hydrophobicity of their side chains. One can assume that the electrophilic group able to interact with the terminal COO- -group of aspartic and glutamic acids is located in the active site of tyrosine phenollyase.
Faleev
N G
NG
Ruvinov
S B
SB
Demidkina
T V
TV
Miagkikh
I V
IV
Gololobov
M Iu
MIu
D000596:Amino Acids / Q000378:metabolism*; D001667:Binding, Competitive; D002954:Citrobacter / Q000201:enzymology*; D007700:Kinetics; D008190:Lyases / Q000037:antagonists & inhibitors*; D013379:Substrate Specificity; D014447:Tyrosine Phenol-Lyase / Q000037:antagonists & inhibitors*
D000596:Amino Acids; D008190:Lyases; D014447:Tyrosine Phenol-Lyase
Vzaimodeĭstvie tirozin-fenol-liazy iz Citrobacter intermedius s aminokislotami i ikh proizvodnymi: faktory, opredeliaiushchie éffektivnost' sviazyvaniia.