Vancomycin serum protein binding determination by ultrafiltration.
Sixty-two serum concentrations were obtained from 12 infected patients enrolled in a vancomycin pharmacokinetic study. Both unbound and total serum vancomycin concentrations were measured using ultrafiltration and a commercial fluorescent polarization immunoassay. Ultrafiltrates were obtained by centrifugation at 1000 X g for ten minutes at room temperature and their assay indicated a range in protein binding from 7.9 to 71 percent. The mean protein binding (mean +/- SD) was 41.95 +/- 14.15 percent. No measurable adsorption of vancomycin onto the ultrafiltration membrane was noted. Orthogonal regression of unbound versus total vancomycin concentrations was described by the equation y = 0.597x-0.362 with a correlation coefficient of 0.948.
Ackerman
B H
BH
Department of Pharmacy Practice, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock 72205.
Taylor
E H
EH
Olsen
K M
KM
Abdel-Malak
W
W
Pappas
A A
AA
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000378:metabolism*; D003404:Creatinine / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D014462:Ultrafiltration; D014640:Vancomycin / Q000097:blood* / Q000493:pharmacokinetics
D001798:Blood Proteins; D012709:Serum Albumin; D014640:Vancomycin; D003404:Creatinine