High-resolution proton magnetic resonance spectroscopy of rabbit brain: regional metabolite levels and postmortem changes.
The changes in 16 cerebral metabolites produced by cardiac arrest and subsequent room temperature autolysis were studied using high-resolution proton nuclear magnetic resonance spectroscopy. Biopsies of rabbit cerebral cortex, cerebral white matter, and cerebellum were quantitatively analyzed for acetate, alanine, gamma-aminobutyric acid, creatine, glutamate, glycine, inositol, lactate, N-acetylaspartate, phosphocreatine, succinate, taurine, and threonine. Of these, N-acetylaspartate and the total creatine pool are the best candidates for use as concentration reference standards linking in vitro to in vivo 1H nuclear magnetic resonance measurements. Both changed little immediately after death, and they varied in a distinctive way among cortex, white matter, and cerebellum.
Petroff
O A
OA
Department of Neurology, Yale University, New Haven, Connecticut 06510.
Ogino
T
T
Alger
J R
JR
D020155:3-Hydroxybutyric Acid; D000085:Acetates / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001329:Autolysis; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D005260:Female; D006885:Hydroxybutyrates / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D010725:Phosphocreatine / Q000378:metabolism; D011180:Postmortem Changes; D011817:Rabbits; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D000085:Acetates; D000596:Amino Acids; D006885:Hydroxybutyrates; D007773:Lactates; D013386:Succinates; D010725:Phosphocreatine; D019344:Lactic Acid; D007294:Inositol; D019802:Succinic Acid; D003401:Creatine; D020155:3-Hydroxybutyric Acid