Isolation and properties of the pituitary gonadotropin from silver carp (Hypophthalmichthys molitrix).
The gonadotropin of silver carp (scGTH) was purified from the pituitary glands in a yield of 8.4 mg/g through extraction with 6% ammonium acetate (pH 5.1)-40% ethanol followed by 80% ethanol precipitation, DEAE-cellulose chromatography and gel filtration. The molecular weight of scGTH is about 32,000 as estimated by gel filtration. Leucine and serine were the two N-termini of scGTH. The amino acid composition of scGTH is rich in aspartic acid/asparagine, glutamic acid/glutamine, valine, proline and half cystine. Its carbohydrate content is 22.2%. Biologically, scGTH significantly stimulated the steroidogenesis in carp testis and ovary in vitro and induced the ovulation in loaches. It also stimulated the 32P-phosphate uptake into 1-day-old chicken testis. Immunologically, scGTH could cross-react with the antiserum against cGTH and vice versa. In both cases parallel displacement curves were displayed.
Chang
Y S
YS
Institute of Biological Chemistry, Academia Sinica, National Taiwan University, Taipei, R.O.C.
Huang
F L
FL
Chen
C T
CT
Lo
T B
TB
D000596:Amino Acids / Q000032:analysis; D000728:Androgens / Q000096:biosynthesis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002347:Carps / Q000502:physiology*; D003530:Cyprinidae / Q000502:physiology*; D003531:Cypriniformes; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D006065:Gonadotropins, Pituitary / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism; D010060:Ovulation / Q000187:drug effects; D010902:Pituitary Gland / Q000032:analysis; D013737:Testis / Q000187:drug effects / Q000378:metabolism
D000596:Amino Acids; D000728:Androgens; D002241:Carbohydrates; D006065:Gonadotropins, Pituitary; D004958:Estradiol