Lanthanide ions as luminescent chromophores for the liquid chromatographic detection of polynucleotides and nucleic acids.
Europium(III) and terbium(III) can be used as luminescent chromophores for the liquid chromatographic detection of certain nucleotides and nucleic acids. The method is dependent upon an energy transfer from the nucleic acid to the lanthanide ion. Of the base moieties, only xanthine, guanine, and thiouridine have appropriate excited state energy levels for efficient energy transfer. The lanthanide ion can be added in a pre- or post-column mode. The applicability of the method was demonstrated for the detection of homologous polynucleotides such as poly X and poly G. The method was also used to detect transfer RNA from Escherichia coli.
Wenzel
T J
TJ
Department of Chemistry, Bates College, Lewiston, ME 04240.
Collette
L M
LM
D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D007476:Ionophores; D008163:Luminescent Measurements; D008674:Metals, Rare Earth; D009696:Nucleic Acids / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis; D011119:Polynucleotides / Q000032:analysis*; D013053:Spectrophotometry; D013725:Terbium
D007202:Indicators and Reagents; D007476:Ionophores; D008674:Metals, Rare Earth; D009696:Nucleic Acids; D009711:Nucleotides; D011119:Polynucleotides; D013725:Terbium