Effect of selenium status on mRNA levels for glutathione peroxidase in rat liver.
To determine the effect of Se status on the level of mRNA for Se-dependent glutathione peroxidase (EC 1.11.1.9), rats were fed either a Se-deficient torula yeast diet (less than 0.02 mg Se/kg diet) or a Se-adequate diet (+0.2 mg Se/kg as Na2SeO3) for greater than 135 d. Liver glutathione peroxidase activity was 0.025 for Se-deficient versus 0.615 EU/mg protein for Se-adequate rats. Total liver RNA and polyadenylated RNA were isolated and subjected to Northern blot analysis using a 700 bp DNA probe from cloned murine glutathione peroxidase. Autoradiography showed that Se-deficient liver had 7-17% of the mRNA for glutathione peroxidase present in Se-adequate liver, suggesting that Se status may regulate the level of mRNA for this selenoenzyme.
Saedi
M S
MS
Department of Nutrition and Food Science, University of Arizona, Tucson 85721.
Smith
C G
CG
Frampton
J
J
Chambers
I
I
Harrison
P R
PR
Sunde
R A
RA
D000199:Actins / Q000235:genetics; D000818:Animals; D005786:Gene Expression Regulation; D005979:Glutathione Peroxidase / Q000235:genetics*; D008099:Liver / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012643:Selenium / Q000172:deficiency*
D000199:Actins; D012333:RNA, Messenger; D005979:Glutathione Peroxidase; D012643:Selenium