[In vitro study of the action of DOTA and DTPA-gadolinium complexes on platelet functions: aggregation and calcium excretion].
Interaction of 2 complexes of Gadolinium (DOTA Gd meglumin and DTPA Gd meglumin) was studied in vitro with regard to platelet function: aggregation and excretion of ATP and of ionised calcium in response to collagen (2.5 Ig/ml). Excretory functions were measured simultaneously with aggregation by bioluminescent techniques using luciferin for the measurements of ATP and aequorin for measurements of ionised calcium. Platelet aggregation and excretion functions, investigated in vitro, were moderately inhibited in the presence of gadolinium complexes. The greatest effects were seen with DTPA Gd meglumin. Complexation of extra-cellular ionised calcium by NMR products in partially responsible for the inhibition of platelet aggregation.
Corot
C
C
INSERM U 37, Bron.
Paul
J
J
Belleville
J
J
Eloy
R
R
D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D005260:Female; D005682:Gadolinium / Q000494:pharmacology*; D019786:Gadolinium DTPA; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009942:Organometallic Compounds / Q000494:pharmacology*; D004369:Pentetic Acid / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*
D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D004369:Pentetic Acid; D000255:Adenosine Triphosphate; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; D005682:Gadolinium; D019786:Gadolinium DTPA; D002118:Calcium
Etude in vitro de l'action des complexes DOTA, DTPA-gadolinium sur les fonctions plaquettaires: agrégation et excrétion calcique.