title: Inhibition of prothrombin activation by factor X and factor IX Gla-peptides.
abstract: In the present study, human factor X and factor IX were each digested with chymotrypsin, and the Gla-peptide from each protein was purified by QAE-Sephadex chromatography. The effect of each Gla-peptide on the activation of human prothrombin by a complex of factor Xa, phospholipid, and calcium was studied using an amidolytic assay for generated thrombin. Prothrombin activation was half-maximally inhibited by factor X Gla-peptide at a concentration of 0.7 microM. Factor IX Gla-peptide was markedly less inhibitory and inhibited this reaction half-maximally at a concentration of 3.7 microM. Kinetic analyses revealed that the factor X Gla-peptide inhibited this reaction in an apparent competitive manner, whereas the factor IX Gla-peptide yielded an exponential Dixon plot. Heat decarboxylation experiments revealed that 3-4 gamma-carboxyglutamic acid residues are critical for the expression of inhibitory activity in each peptide. These studies indicate that, in spite of their structural homology, the ability of each of these Gla-peptides to act as a prothrombinase inhibitor is markedly different.
authors: Department of Pathology, University of New Mexico, School of Medicine, Albuquerque 87131.
authors: P
authors: P
authors: Wildgoose
authors: W
authors: W
authors: Kisiel
mesh_terms: D005164:Factor IX / Q000494:pharmacology*; D005170:Factor X / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D011516:Prothrombin / Q000378:metabolism*; D011517:Prothrombin Time; D013925:Thromboplastin / Q000037:antagonists & inhibitors*
chemical_list: C055980:Factor IX GLA-peptide; C055985:Factor X GLA-peptide; D010446:Peptide Fragments; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X; D013925:Thromboplastin