[Synthesis of peptides catalyzed by papain in organic solvents with minimal water content].
Synthesis of a model peptide, Z-Ala-Val-OBut, catalyzed by papain in acetonitrile containing 0-5% water was studied. Z-Ala-OCH2CONH2 was used as a carboxyl component. The optimal concentration of water was found to be 0.3-0.5%. Under these conditions the reaction rate is 50 to 100 times lower than in water but the yield of the peptide can be as high as 99.5%. Effect of reagent concentrations and temperature on the reaction was also studied.
Mitin
Iu V
IuV
Schellenberger
F
F
D000097:Acetonitriles; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D010206:Papain; D010455:Peptides / Q000138:chemical synthesis*; D012997:Solvents; D014867:Water
D000097:Acetonitriles; D010455:Peptides; D012997:Solvents; D014867:Water; D010206:Papain; C032159:acetonitrile
Sintez peptidov, kataliziruemyĭ papainom, v organicheskikh rastvoriteliakh s minimal'nym soderzhaniem vody.