[Determination of glycosylated hemoglobin HbA1c by liquid phase chromatography].
Glycated hemoglobin A1c assay is performed using HPLC on cation exchange TSK SP 5 PW column. Separation is obtained in 11 minutes using graduated elution with sodium chloride and Bis-Tris buffer. HBA1c is resolved from the other hemoglobins (A1a, A1b, Ao, F, S, C). Reproducibility of method is quite good (cv less than 3%) and correlated with classic low pressure chromatographic analysis using cation exchange resin.
Guyon
F
F
Laboràtoire de Biochimie-Toxicologie, Hôtel-Dieu, Paris.
Potier
M
M
Coignet
M C
MC
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans
D006442:Glycated Hemoglobin
Dosage de l'hémoglobine glycosylée HbA1c par chromatographie en phase liquide.