Amino acid sequence of porcine heart fumarase.
The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.
Sacchettini
J C
JC
Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110.
Frazier
M W
MW
Chiara
D C
DC
Banaszak
L J
LJ
Grant
G A
GA
D000595:Amino Acid Sequence; D000818:Animals; D005649:Fumarate Hydratase / Q000032:analysis*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D010449:Peptide Mapping; D011487:Protein Conformation; D013552:Swine
D005649:Fumarate Hydratase