[Rapid automated synthesis of polydeoxynucleotides].
A rapid automatic method of synthesis of deoxypolynucleotides from 5'-O-dimethoxytritylnucleoside-3'-H-phosphonates is described. An improved construction of synthesizer "Gene-2" adapted for this method has been developed. The modified scheme of synthesis included detritylation with trifluoroacetic acids in dichloromethane, washing with acetonitrile instead of pyridine--acetonitrile mixture and one-step oxidation with iodine solution in acetic acid and pyridine instead of two-step oxidation in the presence of amines. By means of this method, more then 160 polynucleotides containing 8 to 83 monomers were prepared for various biochemical goals including synthesis of promotor 9(260 bp) of the mouse metallothionein-I gene and of promotor and leader sequence (120 bp) of gene of the E. coli alkaline phosphatase.
Kumarev
V P
VP
Baranova
L V
LV
Kobzev
V F
VF
Kuznedelov
K D
KD
Sredin
Iu G
IuG
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis*
D011089:Polydeoxyribonucleotides
Bystryĭ avtomaticheskiĭ sintez polidezoksinukleotidov.