Quantification of soluble serum HLA class I antigens in healthy volunteers and AIDS patients.
A solid-phase enzyme immunoassay (ELISA) has been used to quantify human soluble Class I histocompatibility antigens in serum samples from voluntary blood donors and AIDS patients. Statistical analysis of the results showed significantly raised levels (p less than 0.01) of free HLA Class I in sera from AIDS patients (2.95 +/- 1.80 micrograms/ml) when compared with the blood donors (1.06 +/- 0.6 micrograms/ml). The assay is specific, reproducible and easy to perform. Potential uses of this determination are discussed.
Ferreira
A
A
Unidad de Immunología, Ciudad Sanitaria La Paz, Madrid, Spain.
Villar
M L
ML
Alvarez Cermeño
J C
JC
Revilla
Y
Y
García Rodriguez
M C
MC
Fontán
G
G
González-Porqué
P
P
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans
D006680:HLA Antigens