Autoantibody induced by experimental Onchocerca infection. Effect of different routes of administration of microfilariae and of treatment with diethylcarbamazine citrate and ivermectin.
Hartley guinea pigs were injected with microfilariae (Mf) of Onchocerca lienalis as a model for acute inflammatory responses to Mf in human Onchocerca volvulus infection. IgG autoantibody reactive with a 3 M KCl extract of guinea pig cornea was detected by ELISA in the serum of guinea pigs injected with O. lienalis Mf three or more times sub-conjunctivally, or two or more times subcutaneously. Administration of the microfilaricides diethylcarbamazine citrate and ivermectin did not alter the proportion of animals expressing autoantibody or the mean autoantibody titer. The severity of acute corneal inflammatory reactions to Mf was similar in animals with and without circulating autoantibody. Although autoantibody responses did not correlate with acute corneal inflammatory reactions to dead Mf, the ability of Mf to induce formation of an antibody reactive with a component of autologous cornea suggests that autoimmune mechanisms might participate in chronic onchocercal lesions in the cornea, eg, sclerosing keratitis.
Donnelly
J J
JJ
Department of Ophthalmology, Scheie Eye Institute, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Xi
M S
MS
Haldar
J P
JP
Hill
D E
DE
Lok
J B
JB
Khatami
M
M
Rockey
J H
JH
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D003316:Corneal Diseases / Q000188:drug therapy / Q000276:immunology* / Q000469:parasitology; D004049:Diethylcarbamazine / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000276:immunology; D007559:Ivermectin / Q000627:therapeutic use; D007634:Keratitis / Q000276:immunology; D008297:Male; D008842:Microfilariae / Q000276:immunology; D009854:Onchocerca / Q000276:immunology; D009855:Onchocerciasis / Q000188:drug therapy / Q000276:immunology*
D000909:Antibodies, Helminth; D001323:Autoantibodies; D007074:Immunoglobulin G; D007559:Ivermectin; D004049:Diethylcarbamazine