title: Localization of angiotensin II receptor binding in rabbit brain by in vitro autoradiography.
abstract: Binding of 125I-[Sar1,Ile8] angiotensin II (AII) to sections of brains from both wild and laboratory rabbits was determined by in vitro autoradiography. In the forebrain, specific high density binding was observed in the olfactory bulb, organum vasculosum of the lamina terminalis (OVLT), subfornical organ, median eminence, lateral septum, median preoptic nucleus and hypothalamic paraventricular, supraoptic and arcuate nuclei. In the midbrain, binding of the radioligand was observed in the interpeduncular and parabrachial nuclei, in the locus coeruleus, and ventrolateral pons. In the hind brain, there was dense binding of 125I-[Sar1,Ile8] AII to the nucleus of the solitary tract (NTS) and to both rostral and caudal parts of the reticular formation of the ventrolateral medulla oblongata. Weaker specific binding of the radioligand to the molecular layer of the cerebellum, to the nucleus of the spinal trigeminal tract, dorsal motor nucleus of the vagus, area postema, and to a band of tissue connecting the NTS to the ventrolateral medulla was also observed. Binding of the ligand to circumventricular organs such as the OVLT, subfornical organ, and median eminence suggests that these are sites in the brain of the rabbit at which blood-borne AII may exert influences on the central regulation of fluid balance and pituitary hormone secretion, although AII of neuronal origin could also act at these sites. Binding of the radioligand in several other brain regions suggests that angiotensin II of cerebral origin may be involved in a number of different aspects of brain function in the rabbit. The finding of dense binding in the NTS and ventrolateral medulla, which are involved in autonomic activity and are also sites of catecholamine-containing neurons, raises the possibility of angiotensin interaction with these neurons and involvement in autonomic function.
authors: Department of Medicine, Austin Hospital, University of Melbourne, Heidelberg, Australia.
authors: F A
authors: FA
authors: Mendelsohn
authors: A M
authors: AM
authors: Allen
authors: J
authors: J
authors: Clevers
authors: D A
authors: DA
authors: Denton
authors: E
authors: E
authors: Tarjan
authors: M J
authors: MJ
authors: McKinley
mesh_terms: D000804:Angiotensin II; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D066298:In Vitro Techniques; D008526:Medulla Oblongata / Q000378:metabolism; D008636:Mesencephalon / Q000378:metabolism; D009830:Olfactory Bulb / Q000378:metabolism; D011817:Rabbits / Q000378:metabolism*; D011945:Receptors, Angiotensin / Q000378:metabolism*; D012688:Septum Pellucidum / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D011945:Receptors, Angiotensin; D000804:Angiotensin II