A modified ELISA for the detection of toxic shock syndrome toxin (TSST-1) in staphylococcal isolates.
A modified ELISA for the detection of S. aureus strains producing toxic shock syndrome toxin (TSST-1) is described. Polystyrene balls are coated with specific sheep antibody and incubated with an over-night culture of suspected colonies. Biotinylated second antibody and an Avidin/biotinylated enzyme system are used to obtain an easily readable qualitative reaction.
Fey
H
H
Institut für Veterinär-Virologie der Universität, Bern, Switzerland.
Pfister
H
H
Boerlin
P
P
D001427:Bacterial Toxins; D004768:Enterotoxins / Q000032:analysis* / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012772:Shock, Septic / Q000175:diagnosis*; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism; D018089:Superantigens
D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal