Beta-fibrinogenase from the venom of Agkistrodon p. piscivorus.
1. Beta-fibrinogenase was isolated from the venom of Agkistrodon p. piscivorus by column chromatography on Sephadex G-100, DEAE-Sephacel and by chromatofocusing, with a yield of 2.5 mg of purified enzyme from 1 g of crude venom. 2. The enzyme was homogeneous by SDS and non-SDS disc electrophoresis on polyacrylamide gel at pH 8.3. 3. Beta-fibrinogenase is a glycoprotein possessing both TAME hydrolase and kinin-releasing activities. 4. A mol. wt of approximately 33,500 and an isoelectric point 4.5 was determined. 5. The enzyme is stable to heat treatment and to a pH range of 2-10. 6. Beta-fibrinogenase activity is inactivated by DFP, suggesting that serine is involved in the enzymatic activity. 7. The Michaelis constant (Km) of this enzyme for TAME and inhibition constant (Ki) for DFP were found to be 7.04 X 10(-3) and 4.13 X 10(-3) M, respectively.
Nikai
T
T
Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Katano
E
E
Komori
Y
Y
Sugihara
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003435:Crotalid Venoms / Q000032:analysis*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009113:Muramidase; D013379:Substrate Specificity; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D014106:Tosylarginine Methyl Ester
D003435:Crotalid Venoms; D014106:Tosylarginine Methyl Ester; D009113:Muramidase; D013917:Thrombin