A simple apparatus for controlling nucleation and size in protein crystal growth.
A simple device is described for controlling vapor equilibrium in macromolecular crystallization as applied to the protein crystal growth technique commonly referred to as the "hanging drop" method. Crystal growth experiments with hen egg white lysozyme have demonstrated control of the nucleation rate. Nucleation rate and final crystal size have been found to be highly dependent upon the rate at which critical supersaturation is approached. Slower approaches show a marked decrease in the nucleation rate and an increase in crystal size.
Gernert
K M
KM
NASA/Marshall Space Flight Center, Huntsville, Alabama 35812.
Smith
R
R
Carter
D C
DC
D003460:Crystallization / Q000295:instrumentation*; D004867:Equipment Design; D010316:Particle Size; D011506:Proteins; D012965:Sodium Chloride; D014835:Volatilization
D011506:Proteins; D012965:Sodium Chloride