Demonstration of negative tissue charges by means of polyethyleneimine-metal complexes.
Three polyethyleneimine-metal complexes were synthesized and cytochemically tested for demonstration of negatively charged sites. For this purpose rat cerebral cortex synaptosomes were used. It was established that both types of polyethyleneimine-copper complexes labeled the synaptosomal membrane and synaptic vesicles with electron-dense granules, whereas the polyethyleneimine-lead complex marked the anionic sites with amorphous electron-dense material.
Pfüller
U
U
Staatliches Institut für Immunpräparate und Nährmedien, Berlin, German Democratic Republic.
Ichev
K
K
Ovtscharoff
W
W
Franz
H
H
D000818:Animals; D000838:Anions / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism / Q000648:ultrastructure; D003300:Copper; D006651:Histocytochemistry; D007854:Lead; D011094:Polyethyleneimine; D011095:Polyethylenes; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism / Q000648:ultrastructure
D000838:Anions; D011095:Polyethylenes; D007854:Lead; D003300:Copper; D011094:Polyethyleneimine