Effect of detergents on aspartate ammonia-lyase activity in Escherichia alcalescens.
The effect of twelve detergents on aspartate ammonia-lyase activity of Escherichia alcalescens used for the production of L-aspartic acid was tested. Best permeabilization was found with Triton X-100, Slovafol 910 and Corona, a mixed commercial preparation. In contrast to Triton X-100 and Slovafol 910, a much narrower range of suitable concentrations was observed with Corona.
Malaník
V
V
Research Institute for Biofactors and Veterinary Drugs, Kourim, Czechoslovakia.
Malaníková
M
M
Psenicka
I
I
Sojková
I
I
Marek
M
M
D000642:Ammonia-Lyases / Q000378:metabolism*; D001220:Aspartate Ammonia-Lyase / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D001711:Biotransformation; D002384:Catalysis; D003902:Detergents / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004925:Escherichia / Q000201:enzymology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005650:Fumarates / Q000378:metabolism; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D005650:Fumarates; D013501:Surface-Active Agents; D001224:Aspartic Acid; D011092:Polyethylene Glycols; C032005:fumaric acid; D017830:Octoxynol; D000642:Ammonia-Lyases; D001220:Aspartate Ammonia-Lyase