Density gradients for isolation of mononuclear blood cells for magnesium analysis by electron probe X-ray microanalysis.
We present a mononuclear blood cell (MBC) isolation method for magnesium analysis by electron probe X-ray microanalysis. We compare the inorganic elemental composition of individual MBC isolated by either arabinogalactan (Stractan) or conventional Ficoll-Hypaque density gradients. We find that the MBC isolated with Stractan have the expected cellular element composition, but MBC isolated with Ficoll-Hypaque are contaminated with iodine and sodium. We discuss the source and significance of iodine and sodium contamination of Ficoll-Hypaque isolated MBC and recommend the Stractan separation method for electron probe X-ray microanalysis.
Hook
G R
GR
Surgical Neurology Branch, National Institutes of Neurological and Communicative Disorders and Stroke, Bethesda, MD.
Hosseini
J M
JM
Elin
R J
RJ
D000328:Adult; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D007455:Iodine / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000032:analysis*; D008274:Magnesium / Q000097:blood*; D008297:Male; D012964:Sodium / Q000097:blood; D014961:X-Ray Diffraction
D007455:Iodine; D012964:Sodium; D008274:Magnesium