Isolation of the major O-glycosidically linked oligosaccharides obtained by alkaline borohydride degradation of human meconium glycoproteins.
Neutral and acidic oligosaccharides derived from human meconium glycoproteins by alkaline borohydride degradation have been separated by high-performance liquid chromatography on a Micro-Pak anion-exchange column. In each class, oligosaccharides were purified by normal-phase (neutral and acidic oligosaccharides) and reversed-phase (neutral oligosaccharides) chromatography. Effective separations of neutral oligosaccharides and acidic oligosaccharides were achieved.
Capon
C
C
Laboratoire de Chimie Biologique, Unité Associée au CNRS No. 217, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Cache
P
P
Leroy
Y
Y
Strecker
G
G
Montreuil
J
J
Fournet
B
B
D001894:Borohydrides; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D008470:Meconium / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*
D001894:Borohydrides; D002241:Carbohydrates; D006023:Glycoproteins; D009844:Oligosaccharides