Repair of UV-induced DNA damage in aplastic anaemia: changes after treatment with antilymphocyte globulin (ALG).
The extent of DNA-repair induced by UV-C irradiation was measured in peripheral unstimulated lymphocytes of 24 patients with aplastic anaemia at different stages of disease and compared with the results obtained in 92 controls. As parameter of the DNA-repair synthesis, the incorporation of (3H)thymidine in the presence of 2 mmol/l hydroxyurea (HU) was taken. Of 19 patients tested after treatment with antilymphocyte globulin (ALG), 5 were in complete autologous haemopoietic remission, defined as greater than 1000 granulocytes/mm3, greater than 100,000 platelets/mm3 and a nontransfused haemoglobin value greater than 10 g%. 14 patients were in partial remission, defined as improvement of haemopoietic function, not meeting the criteria for complete remission. 4/5 patients in complete remission had normal DNA-repair synthesis, compared to 4/14 patients in partial remission. In 92 controls, a normal level was found in 70 cases. In 4/5 patients examined at diagnosis and at various intervals after ALG-treatment, DNA-repair synthesis was low at diagnosis. It increased after therapy and paralleled improvement of haemopoietic function to some extent. It is suggested that in aplastic anaemia there are different populations of lymphocytes with differing DNA-repair capacity; ALG treatment seems to favour expansion of the normal population, which is associated with improvement of haemopoietic function.
Kovacs
E
E
Department of Research, University Clinic Kantonsspital, Basel, Switzerland.
Nissen
C
C
Speck
B
B
Signer
E
E
D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000097:blood / Q000235:genetics* / Q000628:therapy; D000961:Antilymphocyte Serum / Q000627:therapeutic use*; D002470:Cell Survival / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013936:Thymidine / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000961:Antilymphocyte Serum; D004247:DNA; D013936:Thymidine