Flow-cytometric assessment of DNA index and percent S phase cells in bronchogenic epidermoid carcinoma.
The DNA index and percent S phase cells were assessed in 45 bronchogenic epidermoid carcinomas from paraffin-embedded retrieved material by flow cytometry. Previously, a comparison between flow cytometric findings from fresh and from paraffin-embedded material was made. The DNA index and the percent S phase cells from fresh and paraffin-embedded material were strongly correlated. Only 3 of the 45 cases were diploid. The DNA index was significantly lower in grade 1 than in grade 2-3 tumors. No correlations were found between DNA index and presence of lymph node metastases, but the percent S phase cells was significantly higher in lymph-node-positive tumors.
Leoncini
L
L
Institute of Pathological Anatomy and Histology, University of Siena, Italy.
Sforza
V
V
Lavarini
E
E
Nuti
S
S
Gotti
G
G
Tosi
P
P
D002283:Carcinoma, Bronchogenic / Q000032:analysis / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008175:Lung Neoplasms / Q000032:analysis / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis
D004273:DNA, Neoplasm