Effect of chronic ethanol feeding on the hepatic secretion of very-low-density lipoproteins.
Using Triton WR-1339 to inhibit plasma metabolism of VLDL, hepatic secretion of VLDL was measured in vivo in control and alcohol-fed rats. The composition of VLDL secreted from livers of chronically alcohol-fed rats was compared with pair-fed controls. The results show that the cholesterol, esterified and nonesterified, as well as the triacylglycerol content of VLDL secreted by the alcohol-fed rats was significantly reduced; protein and phospholipid constituents of the VLDL were unaltered. The apoprotein components of the VLDL showed no apparent difference between the two groups. However, fatty-acid composition of VLDL triacylglycerol and phospholipids show a significant reduction in 20:4 and an increase in 18:2 fatty acids in the alcohol-fed rats. VLDL secretion rates by chronic alcohol-fed rats was reduced significantly compared to the control animals, and this observation has been implicated in the pathogenesis of alcoholic fatty liver.
Venkatesan
S
S
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, U.K.
Ward
R J
RJ
Peters
T J
TJ
D000818:Animals; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D000431:Ethanol / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D008079:Lipoproteins, VLDL / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000032:analysis
D002788:Cholesterol Esters; D005227:Fatty Acids; D008079:Lipoproteins, VLDL; D010743:Phospholipids; D011506:Proteins; D014280:Triglycerides; C030106:very low density lipoprotein triglyceride; D000431:Ethanol; D002784:Cholesterol