DMSO-induced changes in the procoagulant and fibrinolytic activity of B16 melanoma cells: influence on lung colony formation.
In this study DMSO (dimethylsulphoxide) was used as a tool to test the significance of in vitro modifications of procoagulant and fibrinolytic activity of tumor cells for their in vivo metastatic ability. B16 melanoma cells were chosen as the experimental model. After four days' treatment DMSO increased both the procoagulant and fibrinolytic (plasminogen activator) activity of B16 melanoma cells in a dose-related manner. DMSO treated cells showed significantly greater lung colonizing ability than untreated cells. Our results indicate that DMSO treatment in vitro can modulate procoagulant and fibrinolytic activity and the metastatic ability of B16 melanoma cells; however a direct causal relationship between these in vitro and in vivo effects remains to be established.
Casali
B
B
Istituto di Recerche Farmacologiche Mario Negri, Milano, Italy.
Lampugnani
M G
MG
Riganti
M
M
Niewiarowska
A
A
Alessio
G
G
Mussoni
L
L
Semeraro
N
N
Donati
M B
MB
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002460:Cell Line / Q000187:drug effects; D003546:Cysteine Endopeptidases / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005342:Fibrinolysis / Q000187:drug effects*; D008175:Lung Neoplasms / Q000556:secondary*; D008545:Melanoma / Q000503:physiopathology*; D051379:Mice; D009363:Neoplasm Proteins; D010960:Plasminogen Activators / Q000032:analysis; D013925:Thromboplastin / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000187:drug effects
D009363:Neoplasm Proteins; D013925:Thromboplastin; D010960:Plasminogen Activators; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant; D004121:Dimethyl Sulfoxide