title: Activation of a brain-specific protein kinase C subspecies in the presence of phosphatidylethanol.
abstract: Protein kinase C (PKC) is normally activated by diacylglycerol in the presence of Ca2+ and phosphatidylserine. At physiological concentrations of Ca2+, however, phosphatidylethanol, a product of the phospholipase D-catalyzed transphosphatidylation reaction between membrane phospholipids and ethanol, can replace phosphatidylserine, and activate PKC. This mode of activation is most effective for the gamma-subspecies, which is expressed only in central nervous tissue. Phosphatidylmethanol is also effective to some extent. Consideration of these results suggests the possibility that ethanol may exert some effect on signal transduction in this tissue via changes in protein phosphorylation.
authors: Department of Biochemistry, Kobe University School of Medicine, Japan.
authors: Y
authors: Y
authors: Asaoka
authors: U
authors: U
authors: Kikkawa
authors: K
authors: K
authors: Sekiguchi
authors: M S
authors: MS
authors: Shearman
authors: Y
authors: Y
authors: Kosaka
authors: Y
authors: Y
authors: Nakano
authors: T
authors: T
authors: Satoh
authors: Y
authors: Y
authors: Nishizuka
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation; D020404:Glycerophospholipids; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity; D010712:Phosphatidic Acids / Q000494:pharmacology*; D010743:Phospholipids / Q000494:pharmacology; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats
chemical_list: D004075:Diglycerides; D020404:Glycerophospholipids; D007527:Isoenzymes; D010712:Phosphatidic Acids; D010743:Phospholipids; C051521:phosphatidylethanol; D011493:Protein Kinase C; C013965:diolein