Comparative activity in different media of ketoconazole, miconazole and amphotericin B against Candida lusitaniae and sucrose-negative Candida tropicalis.
This study evaluates the susceptibility of sucrose-negative Candida tropicalis and Candida lusitaniae strains to amphotericin B (AMB), miconazole (MCZ) and ketoconazole (KTZ). The susceptibility tests were carried out in different media: Antibiotic Medium 3 (AM-3m) and Earle Minimum Essential Medium (E-MEM) for AMB: Yeast Nitrogen Base (YNB) and E-MEM for imidazole compounds. The minimal fungicidal concentrations (MFCs) of AMB were slightly higher than minimal inhibitory concentration (MICs) except against Candida lusitaniae strains; whereas the MFCs of MCZ and KTZ were higher than the MICs by almost two-fold for all strains tested. AMB was more efficacious against sucrose-negative Candida tropicalis and the MICs were very definite; on the contrary, the MICs with KTZ were difficult to read. The MICs of AMB in E-MEM were essentially the same as those in AM-3m; whereas for KTZ and MCZ determined in YNB the MICs were generally higher than those obtained in E-MEM.
Mignini
F
F
Department of Cellular Biology, Faculty of Pharmacy, University of Camerino, Italy.
Prenna
M
M
Oliveri
S
S
Cammarata
E
E
Falcioni
E
E
Dainelli
B
B
Ripa
S
S
D000666:Amphotericin B / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media; D007654:Ketoconazole / Q000494:pharmacology*; D008825:Miconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D013395:Sucrose; D008825:Miconazole; D000666:Amphotericin B; D007654:Ketoconazole