[Effect of estradiol on polyamine-dependent protein kinase activity of kidney tissue and hormone-dependent mammary gland tumors].
It was demonstrated that cell cytosol and nuclei of estradiol-dependent mammary tumours and kidney of the rat contain a polyamine-dependent protein kinase whose activity is stimulated 2-5 fold by 2 mM spermine and is inhibited by heparin (8-10 micrograms/ml). This protein kinase uses acid proteins (casein) as substrates and ATP and GTP as phosphate sources. The polyamine-dependent protein kinase is eluted from DEAE-cellulose with 0.22-0.24 M NaCl. In cell nuclei the enzyme activity is 3-5 times as high as that in the cytoplasm. In cell nuclei of hormone-dependent tumours and kidney estradiol increases the activity of the polyamine-dependent protein kinase 2-5 times within 30 minutes.
Levashova
Z B
ZB
Morozova
T M
TM
D000818:Animals; D002364:Caseins / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D004789:Enzyme Activation; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007668:Kidney / Q000201:enzymology*; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D009376:Neoplasms, Hormone-Dependent / Q000201:enzymology*; D010052:Ovariectomy; D010766:Phosphorylation; D011073:Polyamines / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002364:Caseins; D011073:Polyamines; D004958:Estradiol; D011494:Protein Kinases
Vliianie éstradiola na aktivnost' poliaminzavisimoĭ proteinkinazy v tkaniakh pochki i gormonzavisimykh opukholiakh molochnykh zhelez.