Identification of [hydroxyproline3]-lysyl-bradykinin released from human kininogens by human urinary kallikrein.
The types of kinins released from purified native, single chain human high and low molecular mass kininogens (HMMKs and LMMKs, respectively) by purified human urinary kallikrein were separated by reverse-phase HPLC and quantitated by the rat uterus bioassay. [Hyp3]-lysyl-bradykinin, a recently discovered kinin, represented up to 58% of the biological activity released from 4 individual HMMK preparations purified from 4 different healthy volunteers. In contrast, the majority of the biological activity released from LMMKs purified from pooled plasma was identified as Lys-bradykinin and [Hyp3]-lysyl-bradykinin represented only 6.4 +/- 3.8%. These findings indicate posttranslation hydroxylation of human kininogens and suggest a preference of HMMKs for this modification.
Maier
M
M
Department of Medical Physiology, University of Vienna, Austria.
Reissert
G
G
Jerabek
I
I
Lottspeich
F
F
Binder
B R
BR
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007609:Kallidin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D007610:Kallikreins / Q000652:urine*; D007704:Kininogens / Q000097:blood*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects
D007704:Kininogens; C054550:bradykinin, (hydroxy-Pro)(3)-Lys-; D007609:Kallidin; D007610:Kallikreins