[Effect of prolactin on the enzymatic activity of thyroid follicular cells during stress due to surgery and subsequent subcutaneous inflammation].
In order to reveal prolactin (Prl) effect on the enzymatic activity of the thyroid follicular cells at a subcutaneous inflammation, 2 series of experiments have been performed on 100 non-inbred male rats with body mass of 150-170 g. In order to produce the inflammation, under ether narcosis celloidin globules are introduced subcutaneously in the abdominal wall. The test animals are given 0.125 mg of "Lactin" (the Soviet preparation of bovine Prl) in 0.5 ml of isotonic solution once every day, beginning immediately after the operation. At the same time the control animals are given 0.5 ml of isotonic solution. The time of observations are--2, 12 h, 1, 2, 3, 5, 10, 15 days after the operation. As a result of quantitative cytochemical investigation of the enzymatic activity in the thyroid follicular cell, multiphasic dynamics of SDG, LDG, G-6-PhDG, NADH-DG, NADPhH-DG, MAO activity and changes in carbohydrate metabolism type at various time of the subcutaneous inflammation are revealed. Daily injections of Prl against the background of the inflammation reduce amplitude of fluctuations of the enzymatic activity in thyrocytes. At late stages of inflammation (up to 2 days) Prl slows down the decrease of SDG, G-6-PhDG, NADH-DG, NADPhH-DG activity and accelerates decrease of LDH activity. At early stages of inflammation (3-15) Prl slows down decrease of NADPhH-DG, NADH-DG activity and decreases LDH and NADH-DG activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Garstukova
L G
LG
Vasil'tsov
M K
MK
D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects; D006651:Histocytochemistry; D007249:Inflammation / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D008297:Male; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats; D013312:Stress, Physiological / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D013961:Thyroid Gland / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D013997:Time Factors
D011388:Prolactin
Vliianie prolaktina na fermentativnuiu aktivnost' follikuliarnykh kletok shchitovidnoĭ zhelezy pri stresse, vyzvannom operatsieĭ i posleduiushchim podkozhnym vospaleniem.