A bovine monoclonal antibody to oestrone/oestradiol prepared by a (murine x bovine) xbovine interspecies fusion.
A heterohybridoma was produced by the fusion of sensitized peripheral blood lymphocytes (PBLs) with a previously derived heteromyeloma, generated by the fusion of bovine PBLs with murine myeloma cells. The sensitized bovine PBLs were collected from a steer immunized with an oestradiol-ovalbumin conjugate. The cell lines resulting from the fusion were screened for the production of bovine antibodies to oestradiol. A stable heterohybridoma was isolated which secreted a bovine IgG1 to oestrone/oestradiol. The use of sensitized PBLs together with heteromyeloma fusion partners has proved to be a reliable and simple way of producing monoclonal antibodies against specific haptens.
Groves
D J
DJ
A.F.R.C. Antibody Development Group, Department of Biochemistry, University of Surrey, Guildford, Great Britain.
Clayton
J
J
Morris
B A
BA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D002459:Cell Fusion; D004958:Estradiol / Q000276:immunology*; D004970:Estrone / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D004970:Estrone; D004958:Estradiol