title: Adherence of Haemophilus somnus to bovine embryos after in vitro exposure.
abstract: Preimplantation bovine embryos were exposed in vitro to Haemophilus somnus to determine whether the bacteria would adhere to zona pellucida-intact embryos or would adhere to or infect zona pellucida-free embryos. The effect of H somnus on in vitro embryonic development also was investigated. After exposure to H somnus and before washing, some of the zona pellucida-intact embryos were held in antibiotic-containing medium. Haemophilus somnus was isolated from 10 to 42 zona pellucida-intact embryos and none of the zona pellucida-free embryos. Haemophilus somnus was not recovered from any of the 32 antibiotic-treated embryos. The coculture system was not compatible with normal embryonic development, and all embryos had begun to degenerate by the end of the 18-hour exposure period.
authors: Department of Microbiology, College of Veterinary Medicine, Auburn University, AL 36849.
authors: M S
authors: MS
authors: Thomson
authors: D A
authors: DA
authors: Stringfellow
authors: L H
authors: LH
authors: Lauerman
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001755:Blastocyst / Q000382:microbiology*; D002417:Cattle / Q000196:embryology*; D006190:Haemophilus / Q000502:physiology*; D066298:In Vitro Techniques; D015044:Zona Pellucida / Q000502:physiology