title: Pulmonary intravascular macrophages and hemodynamic effects of liposomes in sheep.
abstract: We studied the effects of liposomes on the pulmonary circulation of sheep and found a close correlation between liposome retention in the lung and the intravascular macrophages. A test dose of liposomes (5.5 mumol of total lipids) injected intravenously transiently increased pulmonary arterial pressure from 24 +/- 2 to 55 +/- 16 (SD) cmH2O. The pulmonary arterial pressure responses were dose dependent and reproducible. The rise in pulmonary arterial pressure was blocked completely by indomethacin and 75% by a thromboxane synthase inhibitor. Systemic arterial thromboxane B2 concentration increased from a base-line level of less than 50 pg/ml to 250 +/- 130 pg/ml at the peak of the pressor response. Larger doses of liposomes (220 mumol of total lipids) infused intravenously over 1 h increased pulmonary arterial pressure maximally within the first 15 min. Lymph flow increased and lymph protein concentration decreased, suggesting venoconstriction. Over half (62.4 +/- 15.7%) of 111In-labeled liposomes remained in the lung after 2 h. Fluorescence and transmission electron microscopy showed that greater than 90% of the liposomes were associated with mononuclear cells in the lumen of the alveolar wall microvessels. We conclude that liposomes affect pulmonary arterial pressure transiently by a mechanism involving the arachidonate cascade, principally thromboxane. Our observations suggest that a population of pulmonary intravascular macrophages is likely to be the source of the thromboxane and the pulmonary hemodynamic and lymph dynamic changes that occur in a dose-dependent fashion, although interactions between liposomes, leukocytes, or endothelial cells, in addition to the macrophages, have not been completely ruled out. We believe this is the first demonstration that pulmonary intravascular macrophages may be the source of the arachidonate metabolites rather than endothelial cells, neutrophils, or perivascular interstitial cells.
authors: Department of Physiology, University of California, San Francisco 94143-0130.
authors: K
authors: K
authors: Miyamoto
authors: E
authors: E
authors: Schultz
authors: T
authors: T
authors: Heath
authors: M D
authors: MD
authors: Mitchell
authors: K H
authors: KH
authors: Albertine
authors: N C
authors: NC
authors: Staub
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002302:Cardiac Output; D002448:Cell Adhesion; D004305:Dose-Response Relationship, Drug; D007213:Indomethacin / Q000494:pharmacology; D008081:Liposomes / Q000494:pharmacology*; D008168:Lung / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000502:physiology* / Q000648:ultrastructure; D008833:Microcirculation; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011651:Pulmonary Artery / Q000502:physiology; D011652:Pulmonary Circulation; D012756:Sheep; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors
chemical_list: D008081:Liposomes; D013930:Thromboxane-A Synthase; D007213:Indomethacin