Oligosaccharide portion of GM1 enhances process formation by S20Y neuroblastoma cells.
The oligosaccharide portion of ganglioside GM1 was found to enhance neuritogenesis by S20Y murine neuroblastoma cells grown in vitro. The average length of the neurites produced by cells grown in the presence of the oligosaccharide portion of GM1 was comparable to that of cells grown in the presence of intact GM1. The processes of these cells were significantly longer (p less than 0.005, pooled t test) than those of cells grown in the presence of comparable concentrations of sialic acid, lactose, sialyllactose, GD1a, or the oligosaccharide moiety of GD1a. These results suggest that it is the oligosaccharide portion of GM1 that is responsible for the ability of GM1 to enhance process outgrowth by S20Y neuroblastoma cells.
Schengrund
C L
CL
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Prouty
C
C
D000818:Animals; D001369:Axons / Q000473:pathology; D002772:Cholera Toxin / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005677:G(M1) Ganglioside / Q000032:analysis / Q000494:pharmacology*; D005732:Gangliosides / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D012118:Resorcinols; D014407:Tumor Cells, Cultured
D005732:Gangliosides; D009844:Oligosaccharides; D012118:Resorcinols; C021298:ganglioside, GD1a; D005677:G(M1) Ganglioside; D002772:Cholera Toxin; C031389:resorcinol