title: Role of group-specific component (vitamin D binding protein) in clearance of actin from the circulation in the rabbit.
abstract: The possible role of group specific component (Gc) (vitamin D-binding protein) in the clearance of cellular actin entering the circulation was examined with 125I-labeled Gc and actin injected into a rabbit model. Although filamentous F-actin is depolymerized primarily by plasma gelsolin, greater than or equal to 90% 125I-actin injected in either monomeric G- or F-form became complexed eventually with Gc (1:1 molar ratio). Clearance of Gc complexes was much faster (greater than 90% within 5 h) than that of native Gc (t1/2 = 17.2 h). Nephrectomy did not significantly alter the clearance of either Gc or actin. Since Gc complexes are dramatically increased in situations of tissue necrosis such as in fulminant hepatic failure, the current results suggest a crucial role for Gc in sequestration and clearance of released cellular actin.
authors: Department of Microbiology, Medical University of South Carolina, Charleston 29425.
authors: P J
authors: PJ
authors: Goldschmidt-Clermont
authors: H
authors: H
authors: Van Baelen
authors: R
authors: R
authors: Bouillon
authors: T E
authors: TE
authors: Shook
authors: M H
authors: MH
authors: Williams
authors: A E
authors: AE
authors: Nel
authors: R M
authors: RM
authors: Galbraith
mesh_terms: D000199:Actins / Q000097:blood*; D000818:Animals; D001345:Autoradiography; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005110:Extracellular Space / Q000378:metabolism; D007118:Immunoassay; D007525:Isoelectric Focusing; D011817:Rabbits; D014809:Vitamin D-Binding Protein / Q000097:blood / Q000502:physiology*
chemical_list: D000199:Actins; D014809:Vitamin D-Binding Protein