Corneal transmission in whole human eyes.
The transmission of blue-green argon laser light (lambda = 488 nm and lambda = 514.5 nm) through clear healthy corneae was measured by means of a photodiode implanted into whole human donor eyes. The mean corneal transmission in seven eyes (donor age: 32-84 y) was 93.2 +/- 3.2% S.D., range 87-98%. The time between death and measurement ranged from 3.5 to 16 hr. These results indicate that hardly any correction for light loss in a healthy cornea is required in fluorophotometric results obtained with the use of fluorescein (lambda max.exc. = 489 nm, lambda max.em. = 514 nm).
van Best
J A
JA
Department of Ophthalmology, Leiden University Medical Centre, The Netherlands.
Bollemeijer
J G
JG
Sterk
C C
CC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003315:Cornea / Q000502:physiology*; D005260:Female; D006801:Humans; D008027:Light; D008297:Male; D008875:Middle Aged; D055095:Optics and Photonics; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry