High-performance liquid chromatography with electrochemical detection for the determination of nicotine in plasma.
Nicotine in plasma can be determined by high-performance liquid chromatography with electrochemical detection to upper picogram levels. A simple one-step extraction, which requires only 0.1 mL of plasma and is able to recover greater than 85% of nicotine in plasma, is described. The precision and nicotine recovery of the method, and the application of this assay in nicotine-infused animal studies, are presented.
Chien
C Y
CY
Tobacco and Health Research Institute, University of Kentucky, Lexington 40546-0236.
Diana
J N
JN
Crooks
P A
PA
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004563:Electrochemistry; D009538:Nicotine / Q000097:blood* / Q000493:pharmacokinetics
D009538:Nicotine