Expression of the proenkephalin gene in human neuroblastoma cell lines.
Several human tumour cell lines were screened for secretion of proenkephalin-derived peptides with an antiserum directed to its N-terminus, Met-enkephalin-Arg6,Phe7 and for proopiomelanocortin-derived peptides with an antiserum to beta-endorphin. The neuroblastoma SK-N-MC cell line secreted Met-enkephalin-Arg6,Phe7-immunoreactive peptides in relatively high amounts into the culture medium, although processing was not complete and there was no evidence for free Met-enkephalin-Arg6,Phe7. Gene expression was confirmed by the presence of proenkephalin mRNA and proenkephalin-derived polypeptides in extracts of the SK-N-MC cells and also in the neuroblastoma SH-SY5Y cell line. In the latter cells, however, the expression was approximately 3 times lower, there was less processing of proenkephalin and no evidence for secretion.
Folkesson
R
R
Department of Pharmacology, Uppsala University, Sweden.
Monstein
H J
HJ
Geijer
T
T
Påhlman
S
S
Nilsson
K
K
Terenius
L
L
D000310:Adrenal Gland Neoplasms; D002460:Cell Line; D004745:Enkephalins / Q000235:genetics*; D005796:Genes; D006801:Humans; D009447:Neuroblastoma; D009693:Nucleic Acid Hybridization; D010673:Pheochromocytoma; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; C029992:proenkephalin