Determination of phylloquinone and menaquinone in human milk using high performance liquid chromatography.
A high performance liquid chromatographic method for measuring vitamin K in human milk and cow milk is described. The K vitamins were extracted with n-pentane from enzymatic hydrolysate of milk, purified by semipreparative HPLC, and then analyzed by reversed-phase HPLC equipped with a dual electrochemical detector. The amount of phylloquinone and menaquinone-4 in human milk was 2.1 +/- .9 and 1.3 +/- 1 microgram/L, respectively (n = 23). A small amount of menaquinone-6 was detected in both human and cow milk.
Isshiki
H
H
Central Research Institute, Meiji Milk Products Co., Ltd., Tokyo, Japan.
Suzuki
Y
Y
Yonekubo
A
A
Hasegawa
H
H
Yamamoto
Y
Y
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005260:Female; D006801:Humans; D008892:Milk / Q000032:analysis; D008895:Milk, Human / Q000032:analysis*; D014812:Vitamin K / Q000032:analysis*; D010837:Vitamin K 1 / Q000032:analysis*
D014812:Vitamin K; D010837:Vitamin K 1