[Cloning of the genes controlling the biosynthesis of bacitracin in Bacillus licheniformis].
The DNA fragment from bacitracin-producing Bacillus licheniformis strain is cloned on pMX39 vector plasmid in Bacillus subtilis cells. Bacillus subtilis cells carrying the cloned fragment inhibit the growth of bacitracin-sensitive tester strain. The observed inhibition of growth is due to the production by Bacillus subtilis of bacteriocin substance that is identified as bacitracin by TLC-chromatography. In contrast to the data published earlier it is shown that Bacillus subtilis can in fact produce the small amounts of bacitracin. Introduction of the cloned Bacillus licheniformis DNA into Bacillus subtilis cells stimulates this bacitracin production. The restriction site map of the Bacillus licheniformis chromosomal region bearing the cloned fragment is constructed.
Gaĭdenko
T A
TA
Khaĭkinson
M Ia
MIa
D001407:Bacillus / Q000235:genetics* / Q000378:metabolism; D001414:Bacitracin / Q000096:biosynthesis*; D002855:Chromatography, Thin Layer; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D001414:Bacitracin
Klonirovanie genov, kontroliruiushchikh biosintez batsitratsina u Bacillus licheniformis.