Lipid-induced aggregation of phospholipase A2: sucrose density gradient ultracentrifugation and crosslinking studies.
The aggregation behavior of cobra venom (Naja naja naja) phospholipase A2 in the presence of lipids and Ca2+ was examined using ultracentrifugation and crosslinking techniques. Velocity sedimentation experiments were performed in sucrose gradients. The sedimentation coefficients of the cobra phospholipase A2 and various controls, including bovine serum albumin (BSA), malate dehydrogenase, carbonic anhydrase and pancreatic phospholipase A2, were calculated both in the presence and absence of ligands. The monomeric phospholipid, diheptanoylphosphatidylcholine, and the phospholipid analogue, dodecylphosphocholine (DPC), increased the sedimentation coefficient of the cobra phospholipase A2 from 2.2 S to 2.9 S, a value that is consistent with the formation of an enzyme dimer. The control proteins were unaffected by the presence of phospholipid, except for BSA, which apparently binds large amounts of DPC. Crosslinking experiments with glutaraldehyde showed that in the presence of diheptanoylphosphatidylcholine or DPC, the amount of crosslinked enzyme increased. Ca2+ had no effect on the aggregation state of the enzyme as measured by either technique. Both the ultracentrifugation data and crosslinking data are consistent with the hypothesis that the cobra venom phospholipase A2 exists as a dimer or higher-order aggregate in the presence of lipid substrate, although it is yet to be determined whether the functional subunit is a monomer, dimer or higher-order oligomer.
Hazlett
T L
TL
Department of Chemistry, University of California, San Diego, La Jolla 92093.
Dennis
E A
EA
D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002499:Centrifugation, Density Gradient / Q000379:methods; D002794:Choline / Q000031:analogs & derivatives*; D003432:Cross-Linking Reagents; D004546:Elapid Venoms / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology
D003432:Cross-Linking Reagents; D004546:Elapid Venoms; D010713:Phosphatidylcholines; D010767:Phosphorylcholine; D001464:Barium; C038470:1,2-heptanoylphosphatidylcholine; C028810:dodecylphosphocholine; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002794:Choline; D002118:Calcium