Changes of lymphocyte membrane fluidity in rheumatoid arthritis: a fluorescence polarisation study.
Fluorescence polarisation of 1,6-diphenyl-1,3,5-hexatriene was used to study the lymphocyte membrane in rheumatoid arthritis. The increase of polarisation value in the patients (n = 27) compared with healthy controls (n = 32) suggests a decrease of membrane fluidity. Moreover, erythrocyte sedimentation rate (ESR) and plasma fibrinogen concentrations were positively correlated with lymphocyte fluorescence polarisation values (r = 0.66 and r = 0.76 respectively). The results suggest that the changes in lymphocyte membrane fluidity could be involved in the pathogenetic mechanism of rheumatoid arthritis.
Beccerica
E
E
Institute of Rheumatology, Medical Faculty, University of Ancona, Italy.
Piergiacomi
G
G
Curatola
G
G
Ferretti
G
G
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D004161:Diphenylhexatriene; D005260:Female; D005454:Fluorescence Polarization; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D008560:Membrane Fluidity; D008875:Middle Aged
D004161:Diphenylhexatriene