Molecular cloning and nucleotide sequence of the cDNA for human liver glutamate dehydrogenase precursor.
Two cDNA clones (lambda GDHh1 and lambda GDHn61) for glutamate dehydrogenase (GDH) were isolated from a human liver cDNA library in lambda gt11. The clone, lambda GDHh1, was isolated from the library using a synthetic 45mer oligodeoxy-ribonucleotide, the sequence of which was derived from the known amino acid sequence near the NH2-terminus of human liver GDH. Subsequently, lambda GDHn61 was isolated from the same library using lambda GDHh1 as a probe. The inserts of both clones contained an overlapping cDNA sequence for human liver GDH, consisting of a 5'-untranslated region of 70 bp, an open reading frame of 1677 bp, a 3'-untranslated region of 1262 bp and a 15 base poly(A) tract. The predicted amino acid sequence revealed that the human liver GDH precursor consisted of a total of 558 amino acid residues including the NH2-terminal presequence of 53 amino acids. The sequence deduced for the mature enzyme showed 94% homology to the previously reported amino acid sequence of human liver GDH.
Amuro
N
N
Department of Biochemistry, Nippon Medical School, Tokyo, Japan.
Yamaura
M
M
Goto
Y
Y
Okazaki
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004792:Enzyme Precursors / Q000235:genetics*; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data
D004792:Enzyme Precursors; D004247:DNA; D005969:Glutamate Dehydrogenase