title: Cytotoxicity of ketoconazole in malignant cell lines.
abstract: The cytotoxic effects of ketoconazole, an antifungal agent known to have some activity against human prostate cancer, adrenal cancer, and male metastatic breast cancer, were evaluated using colony-growth and clonogenic assays in eight malignant cell lines. The cytotoxicity of ketoconazole showed a dose- and time-dependent pattern, with the following concentrations inhibiting 90% of the growing colonies (IC90): MCF 7 (human breast cancer) 7.25 micrograms/ml, T 47 D (human breast cancer) 9.0 micrograms/ml, MiaPaCa (human pancreatic carcinoma) 10.0 micrograms/ml, COLO 357 (human pancreatic carcinoma), 9.5 micrograms/ml, HCT 8 (human colonic adenocarcinoma) 27.1 micrograms/ml, DU 145 (human prostatic cancer) 40.0 micrograms/ml, AR 42 J (rat pancreatic carcinoma) 9.0 micrograms/ml, and L1210 (murine leukemia) 8.6 micrograms/ml. Since a concentration of 10 micrograms/ml can be achieved in humans, the use of ketoconazole in human malignancies might be worthy of clinical evaluation.
authors: University of California San Francisco, Cancer Research Institute 94143.
authors: C F
authors: CF
authors: Rochlitz
authors: L E
authors: LE
authors: Damon
authors: M B
authors: MB
authors: Russi
authors: A
authors: A
authors: Geddes
authors: E C
authors: EC
authors: Cadman
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007654:Ketoconazole / Q000494:pharmacology*; D051379:Mice; D009369:Neoplasms / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D007654:Ketoconazole