Quantitative analysis of rat brain neurons developing in primary cultures. II. Changes in the distribution of N-CAM associated to neuronal cell surfaces.
Cultured rat fetal brain cells underwent morphological differentiation, as quantitatively described in the companion paper. In the same system, biochemical and immunolabeling studies were performed to analyze the developmental changes in neural cell adhesion molecule (N-CAM) distribution and quantity at the cell surface of neurons. The cell surface-associated N-CAM, related to the culture protein content, remained stable during the two-week period under study, as demonstrated by 125I-protein A binding assays. Immunogold labeling experiments, both in transmission and scanning electron microscopy, indicated a dramatic decrease in N-CAM site density in each membrane compartment, perikarya and neurites. This temporal variation of N-CAM distribution was not accompanied by differences in N-CAM site density between these two membrane compartments. On the other hand, individual perikarya, observed in scanning electron microscopy, showed various levels of labeling. In addition, immunoblot experiments demonstrated the absence of chemical modulation of N-CAM during the period under study, since the high molecular weight (embryonic) form remained dominant. Moreover, an increase in the total N-CAM amount was detected, contrasting with the stable quantity of cell surface-associated N-CAM. This suggested the existence of an N-CAM intracellular pool in cultured neurons. Finally, since the neurite membrane surface area increased 9-fold (companion paper) and since only a 5-fold decrease in N-CAM site density was observed in this compartment, N-CAM supply to neurite membranes was postulated.
Nègre-Aminou
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P
INSERM U. 172, C.N.R.S. UA-1179, Laboratoires de Biologie Cellulaire et de Biochimie, Faculté de Médecine-Nord, Marseille, France.
Massacrier
A
A
Hirn
M
M
Cau
P
P
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism* / Q000502:physiology; D001921:Brain / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D006046:Gold; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D013997:Time Factors
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D006046:Gold