Simultaneous demonstrations of neuropeptide Y gene expression and peptide storage in single neurons of the human brain.
A combination of in situ hybridization for neuropeptide Y mRNA that used a 32P-labeled complementary RNA probe and immunocytochemistry with polyclonal antibodies against neuropeptide Y were applied to human cortical brain samples to simultaneously localize neuropeptide Y and its mRNA. These two techniques allowed simultaneous identification of neuropeptide Y gene expression and peptide storage in single neurons of the human brain.
Chan-Palay
V
V
Department of Neurology, University of Hospital, Zürich, Switzerland.
Yasargil
G
G
Hamid
Q
Q
Polak
J M
JM
Palay
S L
SL
D001921:Brain / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis
D009478:Neuropeptide Y; D012333:RNA, Messenger