1,25-Dihydroxyvitamin D3 enhancement of concanavalin-A induced bovine lymphocyte proliferation: requirement of monocytes.
The effect of 1,25-(OH)2D3 on Concanavalin-A (ConA) mediated bovine lymphocyte proliferation was dependent on the magnitude of the proliferative response. Lymphocytes from cows with high inherent proliferative responses (134,904 +/- 15,488 cpm/well) were unaffected by 1,25-(OH)2D3; whereas, lymphocytes from cows with low inherent proliferative responses (46,317 +/- 6000 cpm/well) were stimulated 75% (P less than 0.01) by 10(-10)M 1,25-(OH)2D3. At low initial proliferation rates, seen with low density cultures enriched for lymphocytes, the effect of 1,25-(OH)2D3 on proliferation changed from inhibition in the absence of added monocytes to stimulation at 18-23% monocyte addition. These results suggest that 1,25-(OH)2D3 possesses both stimulatory and inhibitory effects on ConA-induced proliferation that are monocyte dependent.
Hustmyer
F G
FG
National Animal Disease Center, U.S. Department of Agriculture, Ames, IA.
Nonnecke
B J
BJ
Beitz
D C
DC
Horst
R L
RL
Reinhardt
T A
TA
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007958:Leukocyte Count; D008213:Lymphocyte Activation / Q000187:drug effects*; D009000:Monocytes / Q000276:immunology*
D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D003208:Concanavalin A; D002117:Calcitriol