Purification of galactose oxidase from Dactylium dendroides by affinity chromatography on melibiose-polyacrylamide.
Galactose oxidase is a fungal enzyme which is known to oxidize the C-6 hydroxymethyl of galactose and galactosamine to an aldehyde group. It has been widely used in glycoconjugate research, for example in the labeling of asialoglycoproteins. We have developed a simple affinity purification for galactose oxidase using melibiose-polyacrylamide. This affinity procedure was used to purify the enzyme from ammonium sulfate precipitates of culture filtrates of Dactylium dendroides. The material containing proteases and other contaminants is eluted in the buffer wash. The galactose oxidase is then specifically eluted from the column with buffer containing 0.1 M D-fucose or D-galactose. Using this procedure, the enzyme was also purified from commercial samples of galactose oxidase which contain high proteolytic activity.
Kelleher
F M
FM
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Dubbs
S B
SB
Bhavanandan
V P
VP
D000180:Acrylic Resins; D002846:Chromatography, Affinity; D005656:Fungal Proteins / Q000302:isolation & purification*; D005658:Fungi / Q000201:enzymology*; D005692:Galactose Oxidase / Q000302:isolation & purification*; D008553:Melibiose; D000099166:Polyacrylamides
D000180:Acrylic Resins; D005656:Fungal Proteins; D005692:Galactose Oxidase; D008553:Melibiose; D000099166:Polyacrylamides