title: An analysis of structural aberrations in human sperm chromosomes.
abstract: We have analyzed structural aberrations in 5,000 sperm chromosome complements obtained from 20 men over a 5-yr period by fusion of human sperm with hamster eggs. Detailed data are presented on 366 abnormal cells with 379 analyzable breakpoints. The frequency of cells with structural aberrations ranged from 1.9% to 14.5% among donors; this interindividual variability was statistically significant (p less than 0.0001). In contrast, repeat samples from individual men showed no significant variation over time. The number of sperm chromosome sets processed per hamster egg had no effect on the frequency with which structural aberrations occurred, nor were sperm chromosome abnormalities altered by varying capacitation or culture conditions. The spectrum of structural aberrations observed in human sperm chromosomes and a chi-square analysis of breakpoints based on DNA content are presented. Although human sperm chromosome abnormalities were visualized with a cross-species system, we believe that they represent an inherent, biologically significant phenomenon.
authors: Lawrence Livermore National Laboratory, University of California 94550.
authors: B F
authors: BF
authors: Brandriff
authors: L A
authors: LA
authors: Gordon
authors: D
authors: D
authors: Moore
authors: A V
authors: AV
authors: Carrano
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D006224:Cricetinae; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D009865:Oocytes; D013091:Spermatogenesis; D013094:Spermatozoa / Q000648:ultrastructure*