Nucleotide binding to the rod outer segment rim protein.
The rod photoreceptor outer segment maintains a remarkable morphology. Two of the proteins which have been implicated in the maintenance of this structure are the 240 kDa spectrin-like protein, and the 220 kDa glycoprotein often referred to as the rim protein. We have probed rat rod outer segment proteins with light-activated (azido-labeled) radioactive nucleotides and found a nucleotide binding site(s) on the rim protein which has a preference for guanine nucleotides. Binding to this site is stimulated by the divalent cations zinc, manganese and magnesium, but not calcium. This site is under investigation and may play a role in stabilizing protein structure.
Shuster
T A
TA
Jules Stein Eye Institute, University of California, Los Angeles 90024.
Nagy
A K
AK
Farber
D B
DB
D018528:ATP-Binding Cassette Transporters; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000378:metabolism*; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010786:Photoreceptor Cells / Q000378:metabolism*; D051381:Rats; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
C021961:8-azidoguanosine triphosphate; D018528:ATP-Binding Cassette Transporters; C512840:Abca4 protein, rat; D001386:Azides; D005136:Eye Proteins; D008565:Membrane Proteins; D008345:Manganese; C017040:8-azidoadenosine 5'-triphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D008274:Magnesium; D015032:Zinc