Carbon tetrachloride-induced inhibition of protein kinase C in isolated rat hepatocytes.
Isolated rat hepatocytes exposed to CCl4 showed a dramatic decrease in [32P] incorporation into proteins which was evident as early as 5 min after the haloalkane addition. DEAE cellulose separation of protein kinases present in both particulated and cytosolic fractions of hepatocytes revealed that only the calcium and phospholipids dependent protein kinase C was affected by the treatment with CCl4, while kinases not requiring these factors for their activity were unmodified. Several 4-hydroxyunsaturated aldehydes known to be produced during CCl4-stimulated lipid peroxidation were found to inhibit protein kinase C at micromolar concentrations, suggesting the possibility that peroxidative events might be responsible for the impairment of protein kinase C during CCl4 intoxication.
Poli
G
G
Dipartimento di Medicina ed Oncologia Sperimentale, Universita' di Torino, Italy.
Albano
E
E
Dianzani
M U
MU
Melloni
E
E
Pontremoli
S
S
Marinari
U M
UM
Pronzato
M A
MA
Cottalasso
D
D
D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats
D000447:Aldehydes; D008054:Lipid Peroxides; D010750:Phosphoproteins; D011493:Protein Kinase C