Quantification of plasmalogen, alkylacyl and diacyl glycerophospholipids by micro-thin-layer chromatography.
A method for the determination of plasmalogen, alkylacyl and diacyl glycerophospholipids based on mild alkaline deacylation and acid hydrolysis of plasmalogens on plates with subsequent micro-thin-layer chromatography on silica gel is presented. It is effective in quantifying alkyl and alkenyl analogues of phosphatidylethanolamine and phosphatidylcholine at concentrations up to 0.1% (of the total of the forms in individual classes of phospholipids.
Dembitsky
V M
VM
Institute of Ecology of the Volga River Basin, U.S.S.R. Academy of Sciences, Togliatti.
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D004530:Egg Yolk / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D010955:Plasmalogens / Q000032:analysis*
D010743:Phospholipids; D010955:Plasmalogens