title: Improved sodium hydroxide digestion method without homogenization for extraction of gentamicin from renal tissue.
abstract: Recovery of gentamicin from renal cortical tissue (assayed by fluorescence polarization immunoassay) was highest (P less than 0.05) when using a sodium hydroxide (NaOH) digestion procedure without homogenization (90.0 +/- 5.4%), followed by homogenization with NaOH digestion (85.8 +/- 7.7%) and homogenization with trichloroacetic acid precipitation (84.4 +/- 3.3%). Simple homogenization recovered the least gentamicin (59.0 +/- 5.2%; P less than 0.05).
authors: Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A & M University, College Station 77843.
authors: S A
authors: SA
authors: Brown
authors: K
authors: K
authors: Sugimoto
authors: G G
authors: GG
authors: Smith
authors: F B
authors: FB
authors: Garry
mesh_terms: D000818:Animals; D002417:Cattle; D005454:Fluorescence Polarization; D005839:Gentamicins / Q000302:isolation & purification*; D007668:Kidney / Q000032:analysis*; D012756:Sheep; D012972:Sodium Hydroxide
chemical_list: D005839:Gentamicins; D012972:Sodium Hydroxide