title: Co-existence of immunoreactivity to neuropeptide Y and vasoactive intestinal peptide in non-noradrenergic axons innervating guinea pig cerebral arteries after sympathectomy.
abstract: We have used double-labelling immunofluorescence to examine the coexistence of immunoreactivity (IR) to neuropeptide Y (NPY), tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP) in autonomic neurons innervating guinea pig cerebral arteries. In the rostral circle of Willis of control animals. NPY-IR was detected in 86% of axons with TH-IR (noradrenergic) and 18% of VIP-IR (non-noradrenergic) axons. No axons contained both VIP-IR and TH-IR. Ten to 12 days after bilateral removal of the superior cervical ganglia all TH-IR axons had disappeared. The density of VIP-IR axons was unchanged but now 70% of VIP-IR axons contained NPY-IR. These results show that NPY is not exclusively associated with noradrenergic axons in the cerebral vasculature. Furthermore, NPY levels in non-noradrenergic axons increased following sympathetic denervation.
authors: Department of Anatomy and Histology, School of Medicine, Flinders University of South Australia, Bedford Park.
authors: I L
authors: IL
authors: Gibbins
authors: J L
authors: JL
authors: Morris
mesh_terms: D000818:Animals; D001369:Axons / Q000276:immunology*; D002536:Cerebral Arteries / Q000294:innervation*; D002941:Circle of Willis / Q000294:innervation; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009478:Neuropeptide Y / Q000276:immunology*; D013562:Sympathectomy; D014660:Vasoactive Intestinal Peptide / Q000276:immunology*
chemical_list: D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide