Decrease in intensity of DNA fluorescence caused by interaction between DNA and platinum complexes.
The decrease of DNA fluorescence caused by an impaired capacity of ethidium bromide to intercalate into the DNA reflected structural changes caused in the DNA molecule by its interaction with platinum complexes. This fall in DNA fluorescence was proportional to the length of exposure of DNA to the platinum complexes, and depended on the environment in which the interaction took place. The therapeutically active cisplatinum (cis-DDP) was more efficient to inhibit fluorescence in a solution of 4 X 10(-3) mol NaCl than its therapeutically inactive trans-isomer (trans-DDP). For comparison, the inhibition of DNA fluorescence was also studied in a solution of 10(-2) mol NaClO4. The inhibitory effect was elicited more rapidly, but no difference was found between the two isomers. We concluded that the larger effect of cis-DDP on DNA was induced by the 4 X 10(-3) mol concentration of NaCl. Since also the intracellular concentration of chloride ions is 4 X 10(-3) mol, it cannot be ruled out that the interaction between DNA and cis-DDP and trans-DDP in vivo might be influenced by the intracellular environment.
Rauko
P
P
Cancer Research Institute, Slovak Academy of Sciences, Bratislava; Czechoslovakia.
Povazanová
K
K
Reichelová
V
V
D002712:Chlorides; D002945:Cisplatin; D004247:DNA; D007536:Isomerism; D009994:Osmolar Concentration; D012965:Sodium Chloride; D013050:Spectrometry, Fluorescence
D002712:Chlorides; C034697:transplatin; D012965:Sodium Chloride; D004247:DNA; D002945:Cisplatin