title: Standardization of rye-grass pollen (Lolium perenne) extract. An immunochemical and physicochemical assessment of six candidate international reference preparations.
abstract: Six candidate extracts of Lolium perenne (rye-grass) pollen have been studied in 6 laboratories using a variety of immunochemical and physicochemical techniques. Radioallergosorbent test inhibition, crossed immunoelectrophoresis, crossed radio-immunoelectrophoresis, sodium dodecyl sulphate-polyacrylamide gel electrophoresis combined with immunoblot, thin-layer isoelectric focusing and enzyme-linked immunosorbent assay inhibition were used to evaluate each of the coded extracts. The source materials were also studied for identity and possible contamination by light microscopy. On the basis of these data, the Rye-Grass Working Party recommended to the Steering Committee of the Allergen Standardization Subcommittee of the International Union of Immunological Societies that the extract coded C be chosen as the candidate international reference preparation.
authors: Clinical Immunology Research Unit, Princess Margaret Hospital, Perth, Australia.
authors: G A
authors: GA
authors: Stewart
authors: K J
authors: KJ
authors: Turner
authors: B A
authors: BA
authors: Baldo
authors: A W
authors: AW
authors: Cripps
authors: A
authors: A
authors: Ford
authors: V
authors: V
authors: Seagroatt
authors: H
authors: H
authors: Lowenstein
authors: A K
authors: AK
authors: Ekramoddoullah
mesh_terms: D000485:Allergens / Q000592:standards*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005860:Germany, West; D007123:Immunoelectrophoresis, Two-Dimensional; D007391:International Cooperation; D007525:Isoelectric Focusing; D007558:Italy; D008129:Lolium / Q000276:immunology*; D009426:Netherlands; D010936:Plant Extracts / Q000592:standards; D006109:Poaceae / Q000276:immunology*; D011852:Radioallergosorbent Test; D012015:Reference Standards; D013548:Sweden; D006113:United Kingdom; D014481:United States
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D010936:Plant Extracts; D003101:Collodion