Effects of peptidic glycosaminoglycans complex on human chondrocytes cultivated in three dimensions.
Human chondrocytes from the pelvic joint were cultivated in suspension; under these conditions, after a few days, cells aggregated. These chondrocytes were morphologically differentiated (round shape, situated inside cavities and surrounded by a matrix synthesized during cultivation) and biosynthetically differentiated (synthesis of type II collagen and cartilage proteoglycans (PG) (Bassleer et al. In vitro 22, 115-120, 1986). In this work, we present the metabolic and cellular effects of a peptidic-glycosaminoglycan (P-GAG) complex isolated from calf cartilage and bone marrow. We analyzed the effects of P-GAG on DNA synthesis (appreciated by 3H-thymidine incorporation into DNA), on type II collagen and on PG synthesis analyzed by specific radioimmunoassays. According to its final concentration in culture medium, P-GAG was able to stimulate proliferation or to favor the production of specific components of cartilage matrix, type II collagen and PG.
Bassleer
C
C
Laboratory of Radioimmunology, University of Liege, Belgium.
Gysen
P
P
Bassleer
R
R
Franchimont
P
P
D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006801:Humans; D010455:Peptides / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis
D006025:Glycosaminoglycans; D010455:Peptides; D011509:Proteoglycans; D003094:Collagen; D004247:DNA