title: N-terminal sequence analysis of human placental protein 14, purified in high yield from decidual cytosol.
abstract: Human placental protein 14 (PP14) has been purified in high yield from first trimester decidual cytosol. High-performance liquid chromatography on anion exchange, gel filtration and reverse-phase chromatography were used. The protein obtained is approximately 97% pure with an overall recovery of about 50% from the original tissue extract. The first 24 amino acids of the N-terminal were found to be Met-Asp-Ile-Pro-Gln-Thr-Lys-Gln-Asp-Leu-Glu-Leu-Pro-Lys-Leu-Ala-Gly-Thr-Glu-His - Glu-Met-Ala-Met. PP14 has been characterized in this study to be a dimeric glycoprotein of Mr 60,000, with homologous subunits having an Mr of 28,000.
authors: Department of Obstetrics & Gynaecology, Guy's Hospital, London, U.K.
authors: O M
authors: OM
authors: Westwood
authors: M G
authors: MG
authors: Chapman
authors: N
authors: N
authors: Totty
authors: R
authors: R
authors: Philp
authors: A E
authors: AE
authors: Bolton
authors: N R
authors: NR
authors: Lazarus
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000032:analysis; D003656:Decidua / Q000032:analysis*; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins; D006801:Humans; D008969:Molecular Sequence Data; D011257:Pregnancy Proteins / Q000302:isolation & purification*
chemical_list: D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins