Biologically active calcitonin analogs which have minimal interactions with phospholipids.
A number of peptide hormones have been shown to contain amphipathic helical segments capable of binding to phospholipids. This conformational feature has been associated with increased biological activity of these hormones. We demonstrate, however, that two calcitonin analogs, [Gly8,Ala16]-des-Leu19 salmon calcitonin and des-1-amino-[Ala1,7,Gly8]-des-Leu19 salmon calcitonin have minimal interactions with phospholipids. Neither of these peptides acquire any increased helical content in the presence of dimyristoylphosphatidylglycerol and these peptides have only weak effects in altering the phase transition properties of this lipid. Therefore, although the presence of a phospholipid-induced amphipathic helical sequence may enhance the biological activity, it is not required for activity.
Epand
R M
RM
Department of Biochemistry, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada.
Epand
R F
RF
Orlowski
R C
RC
D002116:Calcitonin / Q000031:analogs & derivatives*; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D010715:Phosphatidylglycerols; D011485:Protein Binding; D011487:Protein Conformation; D013329:Structure-Activity Relationship
D010715:Phosphatidylglycerols; D002116:Calcitonin; C002773:dimyristoylphosphatidylglycerol