title: Dogfish alpha-crystallin sequences. Comparison with small heat shock proteins and Schistosoma egg antigen.
abstract: The amino acid sequences of the alpha-crystallin A and B chains of the dogfish, Squalus acanthias, have been determined. Comparison with alpha-crystallins from other species reveals that charged amino acid replacements have been strongly avoided in the evolution of this lens protein. The homology of alpha-crystallins with the small heat shock proteins is pronounced throughout the major part of the proteins, starting from the position of the first intron in the alpha-crystallin genes, but is also detectable in the amino-terminal sequences of human, Xenopus, and Drosophila small heat shock proteins. In addition, a remarkable short sequence similarity is present only in the amino termini of dogfish alpha B and Drosophila HSP22. The Schistosoma egg antigen p40 turns out to have a tandemly repeated region of homology with the common sequence domain of alpha-crystallins and small heat shock proteins. Comparison of hydropathy profiles indicates the conservation of conformation of the common domains in these three families of proteins. Construction of phylogenetic trees suggests that the alpha A and alpha B genes apparently originated from a single ancestral small heat shock protein gene and indicates that introns have been lost during the evolution of the heat shock protein genes.
authors: Department of Biochemistry, University of Nijmegen, The Netherlands.
authors: W W
authors: WW
authors: de Jong
authors: J A
authors: JA
authors: Leunissen
authors: P J
authors: PJ
authors: Leenen
authors: A
authors: A
authors: Zweers
authors: M
authors: M
authors: Versteeg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002417:Cattle; D003459:Crystallins / Q000032:analysis*; D004284:Dogfish; D004330:Drosophila; D006360:Heat-Shock Proteins / Q000032:analysis*; D015801:Helminth Proteins; D006801:Humans; D008969:Molecular Sequence Data; D014981:Xenopus
chemical_list: D000947:Antigens, Helminth; D003459:Crystallins; D006360:Heat-Shock Proteins; D015801:Helminth Proteins; C055560:p40 egg antigen, Schistosoma