title: [Determination of glycosylated hemoglobin HbA1c by liquid phase chromatography].
abstract: Glycated hemoglobin A1c assay is performed using HPLC on cation exchange TSK SP 5 PW column. Separation is obtained in 11 minutes using graduated elution with sodium chloride and Bis-Tris buffer. HBA1c is resolved from the other hemoglobins (A1a, A1b, Ao, F, S, C). Reproducibility of method is quite good (cv less than 3%) and correlated with classic low pressure chromatographic analysis using cation exchange resin.
authors: Laboràtoire de Biochimie-Toxicologie, Hôtel-Dieu, Paris.
authors: F
authors: F
authors: Guyon
authors: M
authors: M
authors: Potier
authors: M C
authors: MC
authors: Coignet
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans
chemical_list: D006442:Glycated Hemoglobin
vernacular_title: Dosage de l'hémoglobine glycosylée HbA1c par chromatographie en phase liquide.