Preliminary report: immunodiagnosis of pre-type II ostertagiasis.
Ostertagia ostertagi soluble antigens were prepared by gel electrophoresis and electrophoretic transfer onto nitrocellulose for enzyme-linked immunosorbent assays with serum probes. Serologic responses to L3-derived antigen of approximately 32 kDa may be unique and diagnostic for cattle harboring inhibited larvae, or pre-Type II ostertagiasis. Specificity was evaluated by comparing sera from pre-Type II cattle to sera from Type I, uninfected, Fascioloides magna infected, Fasciola hepatica infected or Cooperia oncophora infected cattle.
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USDA, ARS, Animal Parasite Research Laboratory, Auburn, AL 36830.
Klesius
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PH
Williams
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JC
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay; D010028:Ostertagia / Q000276:immunology*; D010029:Ostertagiasis / Q000175:diagnosis / Q000662:veterinary*; D011237:Predictive Value of Tests; D014252:Trichostrongyloidiasis / Q000662:veterinary*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth