Relationship of 65Zn absorption kinetics to intestinal metallothionein in rats: effects of zinc depletion and fasting.
Intestinal 65Zn transport and metallothionein levels were examined in rats fed zinc-adequate and zinc-deficient diets and in rats subjected to an overnight fast. 65Zn uptake by intestines perfused with 1.5 microM 65Zn was greater in both zinc-deficient and fasted groups than in the control group. Mucosal retention of 65Zn was also greater in the zinc-deficient group but not in the fasted group. The greater 65Zn uptake in the fasted group was associated with a compartment that readily released 65Zn back into the lumen. Kinetic analysis of the rate of 65Zn transfer to the vascular space (absorption) showed that 65Zn absorption involved approximately 3% of mucosal 65Zn in a 40-min perfusion period. The half-life (t1/2) of this mucosal 65Zn rapid transport pool corresponded directly to changes in intestinal metallothionein levels. Both metallothionein and t1/2 were higher in the fasted group and lower in the zinc-deficient group than in controls. While the rate of 65Zn transport from this rapid transport pool decreased with increasing metallothionein level, the predicted pool size increased when the metallothionein level was elevated by fasting. These results indicate that the rate of zinc absorption is inversely related to intestinal metallothionein levels, but the portion of mucosal 65Zn available for absorption is directly related to intestinal metallothionein.
Hoadley
J E
JE
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611.
Leinart
A S
AS
Cousins
R J
RJ
D000284:Administration, Oral; D000818:Animals; D001692:Biological Transport; D005508:Food Deprivation; D006207:Half-Life; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000502:physiology*; D051381:Rats; D015032:Zinc / Q000008:administration & dosage / Q000172:deficiency / Q000493:pharmacokinetics*; D015037:Zinc Radioisotopes
D015037:Zinc Radioisotopes; D008668:Metallothionein; D015032:Zinc