Development of standard materials for use with the hematofluorometer.
Two methods for preparing erythrocytes were evaluated for the purpose of developing stable, blood-based standard materials for the hematofluorometer. Erythrocytes washed with citrate-phosphate-dextrose solution and reconstituted with platelet-free plasma were stable for 12 weeks. Plasma-free erythrocytes washed with isotonic saline and resuspended in citrate-glycerol solution were stable for 18 weeks when stored at 4 degrees C. Consequently, plasma-free erythrocytes were successfully used as proficiency test specimens in the New York State Department of Health's Clinical Laboratory Evaluation Program for hematofluorometer users.
Parsons
P J
PJ
Laboratory of Inorganic and Nuclear Chemistry, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
Meola
J R
JR
Mitchell
D G
DG
D000818:Animals; D002648:Child; D004912:Erythrocytes / Q000032:analysis*; D005260:Female; D005470:Fluorometry; D006041:Goats / Q000097:blood; D006400:Hematocrit; D006801:Humans; D007854:Lead / Q000097:blood; D008297:Male; D011166:Porphyrins / Q000097:blood*; D011524:Protoporphyrins / Q000097:blood*; D011786:Quality Control; D012016:Reference Values
D011166:Porphyrins; D011524:Protoporphyrins; D007854:Lead