Increased calmodulin synthesis in the pre-replicative phase of rat liver regeneration.
A cytosolic calmodulin wave has been described in the pre-replicative phase of rat liver regeneration. Here we demonstrate that this calmodulin increase is reduced by injection of cycloheximide (translation inhibitor) and actinomycin D (transcription inhibitor). In addition, we found that calmodulin mRNA levels are increased during the early pre-replicative period of liver regeneration, and this increase is affected by actinomycin D. Together, these results strongly indicate that the calmodulin wave observed in the pre-replicative phase of liver regeneration is due to de novo synthesis of this protein.
Piõl
M R
MR
Facultat de Medicina, Universitat de Barcelona, Departament de Biologia Cellular i Anatomia Patològica, Spain.
Berchtold
M W
MW
Bachs
O
O
Heizmann
C W
CW
D000818:Animals; D002147:Calmodulin / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007399:Interphase; D008115:Liver Regeneration; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D002147:Calmodulin; D012333:RNA, Messenger; D003609:Dactinomycin; D003513:Cycloheximide