title: Organotypic culture of central histamine neurons.
abstract: Organotypic cultures of histaminergic tuberomammillary (TM) neurons were grown using explants obtained from newborn rats. The cultures were examined after immunohistochemical localization of the histamine synthetic enzyme, L-histidine decarboxylase (HDC). The morphological properties of the somata, dendrites and axons of HDC-immunoreactive TM neurons in organotypic culture were virtually indistinguishable from those seen in situ. Extensive plexuses of HDC-immunopositive axons, including growth cones, were seen within the hypothalamus, the plasma surrounding the explant and co-cultured hippocampus. Organotypic cultures of TM histamine neurons, and co-cultures with their targets, provide a useful model system for studying several aspects of central histaminergic neurobiology.
authors: Department of Neurosurgery, University of Zürich, Switzerland.
authors: P B
authors: PB
authors: Reiner
authors: B
authors: B
authors: Heimrich
authors: F
authors: F
authors: Keller
authors: H L
authors: HL
authors: Haas
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000032:analysis; D002478:Cells, Cultured; D006624:Hippocampus / Q000032:analysis / Q000166:cytology*; D006632:Histamine / Q000032:analysis*; D006640:Histidine Decarboxylase / Q000032:analysis; D007031:Hypothalamus / Q000032:analysis / Q000166:cytology*; D007150:Immunohistochemistry; D051381:Rats
chemical_list: D006632:Histamine; D006640:Histidine Decarboxylase