Differential localization of the high- and low-molecular weight variants of MAP2 in the developing retina.
Microtubule-associated protein 2 (MAP2) occurs in developing mammalian neuronal tissue as both high (280 kDa)- and low (70 kDa)-molecular weight forms with temporally regulated expression. We have studied the developing avian retina with a monoclonal antibody that recognizes both the high- and low-molecular weight forms of MAP2 and a second monoclonal antibody that recognizes only high-molecular weight MAP2. The developmentally regulated, low-molecular weight protein, MAP2c, has a more widespread distribution in the embryonic avian retina than high-molecular weight MAP2. Our results suggest that MAP2c is the first form of MAP2 to appear in differentiated embryonic retinal neurons, and that the high-molecular weight isoforms of MAP2 appear only later when they may confer stability to neuronal processes.
Tucker
R P
RP
Friedrich Miescher-Institute, Basel, Switzerland.
Binder
L I
LI
Matus
A I
AI
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D003370:Coturnix; D005314:Embryonic and Fetal Development; D008869:Microtubule-Associated Proteins / Q000378:metabolism* / Q000502:physiology; D008970:Molecular Weight; D012160:Retina / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D008869:Microtubule-Associated Proteins