Molecular size variations in an immunoprotective protein complex among isolates of Anaplasma marginale.
A major surface protein complex from the Florida isolate of Anaplasma marginale has been previously shown to induce protection in immunized cattle and has been proposed as the basis of a subunit vaccine against anaplasmosis. This complex in the Florida isolate is composed of two noncovalently associated polypeptides with molecular masses of 105 and 100 kilodaltons (kDa). The analogous protein complex from four geographically different isolates of A. marginale was immunoprecipitated and compared with the protein complex of the Florida isolate. The polypeptides of the complex varied in apparent molecular mass among the isolates. By using antibodies recognizing epitopes on each polypeptide of the Florida isolate, the antigenic identity of the polypeptides in the analogous complexes was determined. The polypeptides recognized by the neutralizing monoclonal antibody 22B1, which recognizes a 105-kDa polypeptide in the Florida isolate, ranged from 70 to 100 kDa in the other isolates. Those polypeptides recognized by rabbit antiserum R911, which recognizes a 100-kDa polypeptide in the Florida isolate, ranged from 97 to 100 kDa. The surface-exposed peptides in the complexes were compared by limited enzymatic digestion to assess structural homology among isolates. Despite the marked variations in molecular weight, there were conserved peptides between the 22B1-reactive polypeptides and between the R911-reactive peptides. Determination of the role of the conserved peptides in inducing immunity will be critical in the application of these polypeptides as the basis of a subunit vaccine for bovine anaplasmosis.
Oberle
S M
SM
Department of Infectious Diseases, University of Florida, Gainesville 32610.
Palmer
G H
GH
Barbet
A F
AF
McGuire
T C
TC
D000709:Anaplasma / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology; D011110:Polymorphism, Genetic; D011233:Precipitin Tests
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins; D010455:Peptides