Mutual recognition between polymerized liposomes: enzyme and enzyme inhibitor system.
In order to examine the usefulness of polymerized liposomes as a model for cell membranes, a mutual recognition phenomenon between different liposomes on which complementary ligands were attached was examined. We used trypsin- and soybean trypsin inhibitor (STI)-carrying polymerized liposomes to attain high sensitivities. The STI which was immobilized on the polymerized mono-dienoylphosphatidylcholine liposome showed a definite inhibitory effect on the catalytic activity of the trypsin which was immobilized on another polymerized liposome, whereas the inhibitory effect of the STI which was immobilized on the di-dienoylphosphatidylcholine liposome was much smaller than that of the mono-dienoylphosphatidylcholine system because of the larger rigity of the di-dienoylphosphatidylcholine liposome. These results suggest that the mutual recognition between complementary ligands can be realized by using polymerized liposomes with a physical stability and moderate deformability as their carriers.
Kitano
H
H
Department of Polymer Chemistry, Kyoto University, Japan.
Kato
N
N
Tanaka
N
N
Ise
N
N
D002450:Cell Communication; D002462:Cell Membrane / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008024:Ligands; D008081:Liposomes; D008565:Membrane Proteins / Q000502:physiology*; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry; D011108:Polymers; D011485:Protein Binding; D013696:Temperature; D014357:Trypsin; D014361:Trypsin Inhibitors
D008024:Ligands; D008081:Liposomes; D008565:Membrane Proteins; D011108:Polymers; D014361:Trypsin Inhibitors; D014357:Trypsin