Isolation of neurosecretory granules containing vasopressin and MSEL-neurophysin from guinea pig neurointermediate pituitary.
Neurosecretory granules have been isolated from rat and guinea pig neurointermediate pituitaries and their contents have been analyzed by reverse-phase high-pressure liquid chromatography and polyacrylamide gel electrophoresis. Granule components have been compared with synthetic neurohypophysial hormones and chemically characterized neurophysins. In rat granules, oxytocin, arginine vasopressin and MSEL- and VLDV-neurophysins have been identified. In isolated guinea pig granules, only arginine vasopressin and mature MSEL-neurophysin have been found. From these results it can be concluded that both the "dibasic" cleavage between vasopressin and MSEL-neurophysin and the "monobasic" cleavage between MSEL-neurophysin and copeptin occur within the granule compartment. Previous isolation from frozen guinea pig glands of a partially processed precursor encompassing MSEL-neurophysin and copeptin suggests a two-step processing of the three-domain vasopressin precursor, each involving a distinct enzymic system.
Fielbal
A M
AM
Laboratory of Biological Chemistry, University of Paris, France.
Rouillé
Y
Y
Chauvet
J
J
Chauvet
M T
MT
Acher
R
R
D000818:Animals; D001127:Arginine Vasopressin / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000032:analysis; D006168:Guinea Pigs; D009481:Neurophysins / Q000302:isolation & purification*; D010121:Oxytocin / Q000302:isolation & purification; D010904:Pituitary Gland, Posterior / Q000032:analysis*; D011498:Protein Precursors / Q000302:isolation & purification*; D051381:Rats
D009481:Neurophysins; D011498:Protein Precursors; D001127:Arginine Vasopressin; D010121:Oxytocin; C055597:VLDV neurophysin