Content of sitosterol, cholestanol, and cholesterol in very low density lipoproteins of rat liver perfusate.
Sterol specificity in synthesis of very low density lipoproteins in the rat liver was studied by liver perfusion after injection in vivo of sterols as double isotopes. The amounts of sterols recovered in the very low density lipoprotein fraction of the perfusate were compared with the microsomal contents, using both double isotope ratio technique and quantitation by gas chromatography. The secretion of sitosterol and cholestanol was 0.72 and 0.88 of that of cholesterol, respectively. Variations of microsomal sterol concentrations did not affect the principal results. Relative to cholesterol, one-third more of injected sitosterol was present in the liver after 24 h. Sitosterol was excreted in the bile at one-fourth the rate of cholesterol and to a larger extent as free sterol compared with cholesterol.
Boberg
K M
KM
Institute of Clinical Biochemistry, University of Oslo, Norway.
Skrede
S
S
D000818:Animals; D002777:Cholestanols / Q000008:administration & dosage / Q000032:analysis*; D002784:Cholesterol / Q000008:administration & dosage / Q000032:analysis*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008079:Lipoproteins, VLDL / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008862:Microsomes, Liver / Q000032:analysis; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012855:Sitosterols / Q000008:administration & dosage / Q000032:analysis*; D014461:Ultracentrifugation
D002777:Cholestanols; D008079:Lipoproteins, VLDL; D012855:Sitosterols; D002784:Cholesterol