Synthesis of cyclic oligonucleotides by a modified phosphotriester approach.
Evidence will be presented to show that the allyl group is suitable for the protection of a 3'-terminal phosphodiester function. The latter will be demonstrated by the synthesis, via a phosphotriester approach, of two cyclic tetraribonucleotides [r(AAAA) and r(UAMe2UAMe2)], two cyclic hexadeoxyribonucleotides [d(CGCGCG) and d(TAAAAA)] and a cyclic octadeoxyribonucleotide [d(CGTGCGTG)].
de Vroom
E
E
Gorlaeus Laboratory, Leiden, The Netherlands.
Broxterman
H J
HJ
Sliedregt
L A
LA
van der Marel
G A
GA
van Boom
J H
JH
D004355:Drug Stability; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009712:Nucleotides, Cyclic / Q000138:chemical synthesis*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010710:Phosphates
D007202:Indicators and Reagents; D009712:Nucleotides, Cyclic; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D010710:Phosphates