[Elongation of oligonucleotides covalently bound to the active center of RNA polymerase].
Elongation (mediated by RNA polymerase and NTPs) of the primer oligonucleotide residues, covalently fixed near the active centre of RNA polymerase, has been studied. Hepta- and octanucleotide residues covalently attached to beta-subunit could not be elongated (evidently, because the translocation step is prevented), whereas the same residues attached to sigma-subunit were easily elongated. It was concluded that ease of the oligonucleotide residue elongation is due to the dissociation of sigma-subunit from the transcription complex. The mechanism of this dissociation is discussed.
Zaĭchikov
E F
EF
Tsarev
I G
IG
D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000235:genetics* / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
D009841:Oligonucleotides; D012321:DNA-Directed RNA Polymerases
Izuchenie élongatsii oligonukleotidov, kovalentno fiksirovannykh v aktivnom tsentre RNK-polimerazy.