A screening method for the simultaneous determination of putrescine, cadaverine, histamine, spermidine and spermine in fish by means of high pressure liquid chromatography of their 5-dimethylaminonaphthalene-1-sulphonyl derivatives.
A simple and rapid method is described for the extraction, derivatization and subsequent determination by means of high pressure liquid chromatography of putrescine, cadaverine, histamine, spermidine and spermine as their 5-dimethylaminonaphthalene-1-sulphonyl derivatives. The amines are extracted from the fish material by an aqueous trichloroacetic acid solution and derivatized by means of 5-dimethylaminonaphthalene-1-sulphonylchloride (dansyl chloride). The reaction mixture is then injected on a RP-8 column using gradient elution to separate the amine derivatives. Use of the method results in a considerable saving with respect to both time and costs.
Rosier
J
J
Pharmaceutical Institute, State University of Ghent, Belgium.
Van Peteghem
C
C
D000818:Animals; D002103:Cadaverine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003619:Dansyl Compounds; D003959:Diamines / Q000032:analysis*; D005399:Fishes; D006632:Histamine / Q000032:analysis*; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D011700:Putrescine / Q000032:analysis*; D013095:Spermidine / Q000032:analysis*; D013096:Spermine / Q000032:analysis*
D003619:Dansyl Compounds; D003959:Diamines; D007202:Indicators and Reagents; D013096:Spermine; D006632:Histamine; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine