title: Monoclonal antibodies reacting with gliadin as tools for assessing antigenic structure responsible for exacerbation of celiac disease.
abstract: Monoclonal antibodies reactive with gliadin were prepared by fusion of spleen cells isolated from gliadin-immunized BALB/c mice with myeloma cells. The reactivity of mAbs with different preparations of gliadin and their enzymatic digest were measured using ELISA method. The mAb produced by GL 1 clone was shown to react preferentially with alpha-gliadin and its enzymatic digest.
authors: Institute of Molecular Genetics, Czechoslovak Academy of Science, Prague.
authors: S
authors: S
authors: Stoyanov
authors: H
authors: H
authors: Tlaskalová-Hogenová
authors: P
authors: P
authors: Kocna
authors: H
authors: H
authors: Kristofová
authors: P
authors: P
authors: Fric
authors: W T
authors: WT
authors: Hekkens
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002446:Celiac Disease / Q000209:etiology*; D005903:Gliadin / Q000009:adverse effects / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D010940:Plant Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D010940:Plant Proteins; D005903:Gliadin