Dose-time relationship of the development of preneoplastic liver lesions induced in rats with low doses of N-nitrosodiethanolamine.
The carcinogenic efficiency of very low doses of N-nitrosodiethanolamine (NDELA), an N-nitroso compound of environmental significance, was assessed by administering it to male Sprague-Dawley rats at five different dose levels: 0.2, 0.63, 1.5, 6 and 25 mg/kg b.w./day in the drinking water. Quantitation of the numbers and size of liver foci positive for glucose-6-phosphate dehydrogenase (G6PDH) by morphometric methods revealed a good correlation between the dose and duration of carcinogen treatment and the extent of G6PDH-positive foci development. Thus the area density increased proportional to time and dose. The dose-time relation for the induction of 1% G6PDH-positive liver tissues assessed as a double logarithmic plot gives a straight line with the same characteristics as that which results when the induction of liver tumors is evaluated.
Zerban
H
H
Institut für Experimentelle Pathologie, Deutsches Krebsforschungszentrum, Heidelberg, FRG.
Preussmann
R
R
Bannasch
P
P
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004052:Diethylnitrosamine / Q000031:analogs & derivatives* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D002273:Carcinogens; C014129:N-nitrosodiethanolamine; D004052:Diethylnitrosamine