The adenovirus tripartite leader sequence can alter nuclear and cytoplasmic metabolism of a non-adenovirus mRNA within infected cells.
All mRNAs encoded by the adenovirus major late transcription unit share a common 5' noncoding region, 200 nucleotides in length, termed the tripartite leader sequence. To assess function of the tripartite leader, recombinant viruses were prepared which carried either a bona fide herpes simplex virus thymidine kinase gene or a modified thymidine kinase gene whose normal 5' noncoding domain was replaced with the adenovirus leader sequence. The tripartite leader simultaneously decreased the nuclear half-life and increased the cytoplasmic half-life of the thymidine kinase-specific mRNA. The tripartite leader stabilized the non-adenovirus mRNA only within the environment of an adenovirus-infected cell during the late phase of the infectious cycle.
Moore
M A
MA
Department of Molecular Biology, Princeton University, NJ 08544.
Shenk
T
T
D000260:Adenoviruses, Human / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013937:Thymidine Kinase / Q000235:genetics*
D004274:DNA, Recombinant; D012333:RNA, Messenger; D012367:RNA, Viral; D013937:Thymidine Kinase