Production of an active urokinase by leukemia cells: a novel distinction from cell lines of solid tumors.
A new screening test is described which enabled rapid determination of the proportion of single-chain and two-chain urokinase produced in the culture supernatants of 18 human cell lines. A clear distinction was found between two groups of cell lines: cells derived from ten solid tumors produced almost exclusively single-chain proenzyme, while the majority of the enzyme found in cultures of eight leukemia cell lines was in the active, two-chain form.
Stephens
R
R
Department of Virology, University of Helsinki, Finland.
Alitalo
R
R
Tapiovaara
H
H
Vaheri
A
A
D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis*; D007942:Leukemia, Experimental / Q000201:enzymology; D014407:Tumor Cells, Cultured / Q000201:enzymology*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis*
D007527:Isoenzymes; D014568:Urokinase-Type Plasminogen Activator