Surface sugar binding components of bovine spermatozoa as evidence by fluorescent neoglycoproteins.
In the present study, the topographical distribution of carbohydrate binding sites on the plasma membrane of bovine epididymal spermatozoa was investigated using 15 different fluorescent neoglycoproteins and asialoglycoproteins. With mannose-bovine serum albumin (BSA)-fluoresceinthiocarbamyl (FTC), mannose-6-phosphate-BSA-FTC, lactose-BSA-FTC, maltose-BSA-FTC, asialolactoferrin-FTC and asialotransferrin-FTC a marked fluorescence was observed in the postacrosomal area. These results further substantiate the concept that carbohydrate binding sites of the spermatozoan plasma membrane and corresponding carbohydrates of the zona pellucida play a significant role in gamete interactions.
Sinowatz
F
F
Institute for Veterinary Anatomy II, Ludwig-Maximilians-University Munich, Federal Republic of Germany.
Gabius
H J
HJ
Amselgruber
W
W
D000818:Animals; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D006023:Glycoproteins / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D012710:Serum Albumin, Bovine; D013094:Spermatozoa / Q000378:metabolism*
D005452:Fluoresceins; D006023:Glycoproteins; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate