Influenza virus ISCOMs: biochemical characterization.
Immunostimulating complexes (ISCOMs) have been prepared from influenza A virus envelope glycoproteins, i.e. haemagglutinin (HA) and neuraminidase (NA). An ISCOM consists of a matrix, which is the micellar form of the glycoside, Quil A, in hydrophobic interaction with both the envelope glycoproteins (HA/NA). The Quil A bound to the ISCOM amounted to 50 micrograms mg-1 (5%) of ISCOM protein. ISCOMs were morphologically identified as symmetrical cage-like structures of approximately equal to 40 nm in diameter with hexagonal or pentagonal subunits of approximately equal to 12 nm. The sedimentation coefficient was approximately equal to 19 S as compared to 30 S for the glycoprotein micelles. The biological activities of the HA and NA are preserved in both ISCOMs and micelles.
Sundquist
B
B
National Veterinary Institute, Division of Vaccine Research, Uppsala, Sweden.
Lövgren
K
K
Höglund
S
S
Morein
B
B
D000276:Adjuvants, Immunologic / Q000032:analysis*; D002498:Centrifugation; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis; D006386:Hemagglutination Tests; D008823:Micelles; D008854:Microscopy, Electron; D009975:Orthomyxoviridae / Q000276:immunology* / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology*
D000276:Adjuvants, Immunologic; D006023:Glycoproteins; D008823:Micelles; D014759:Viral Envelope Proteins