Establishment and characterization of a new human melanoma cell line (HU 214) with a high growth potential and stable properties.
A human melanoma cell line (HU 214) with high growth potential was established from a lymph node metastasis of a patient with advanced cutaneous melanoma. The cells of this line were able to grow in monolayer (according to the Rosenblum technique) and in agar (according to the Courtenay-Mills method), and formed tumors when injected in nude mice. The line has been maintained in culture for more than 47 passages. The cell cultures were periodically characterized (every 6-8 passages) by immunohistochemistry using a panel of monoclonal antibodies (MoAbs) including MoAbs against tumor-associated antigens (antimelanoma, antiglioma and anti-LLA), against vimentin, and against major histocompatibility antigens, and including also Ki 67, a MoAb which reacts with a nuclear antigen associated with cell proliferation. The results of this characterization indicate that we have established a human melanoma cell line with a stable antigenic phenotype during subculturing, poorly differentiated cells, and a high growth potential.
Molino
A
A
Department of Medicine, University of Verona.
Stevanoni
G
G
Lorenzi
P
P
Dipasquale
B
B
Colombari
R
R
Chilosi
M
M
Cetto
G L
GL
Forni
G
G
Gerosa
M
M
Tridente
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D008545:Melanoma; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012878:Skin Neoplasms; D014746:Vimentin / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006680:HLA Antigens; D014746:Vimentin