Inhibition of granulocyte-mediated release of oxygen free radicals following glutathione depletion.
Treatment of granulocytes freshly isolated from the blood of healthy human adult donors with phorone (diisopropylidene acetone) resulted in a clear depletion of intracellular glutathione without affecting cell viability. Upon stimulation of the phagocytes with phorbol myristate acetate (PMA), however, the release of .O2- and H2O2 by glutathione-depleted granulocytes was inhibited by 50 or 95% as compared to control cells, respectively. These findings may be of toxicological importance, as many xenobiotics are known to interact with glutathione (GSH)-dependent cellular defense mechanisms and may thus influence the phagocytic activity of granulocytes.
Younes
M
M
Institute of Toxicology, Medical University of Lübeck, F.R.G.
Röbke
A
A
D000328:Adult; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007659:Ketones / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D010587:Phagocytosis
D005609:Free Radicals; D007659:Ketones; C018637:phorone; D005978:Glutathione; D010100:Oxygen; D004121:Dimethyl Sulfoxide