Quantitative determination of phenelzine in human fluids by gas chromatography with nitrogen specific detection.
A quantitative gas chromatographic assay for the determination of phenelzine in plasma or other fluids is presented. A stable derivative is formed by cyclization with acetylacetone, and the derivative is separated, purified, and concentrated by liquid-liquid solvent extraction. Analysis of the extracts is by capillary gas chromatography with nitrogen specific detection. Detection limits of less than 1 ng/mL and CVs of 5% at the 10-ng/mL level are attainable with a 2-mL sample and benzylhydrazine as the internal standard.
Lichtenwalner
M
M
National Medical Services, Willow Grove, Pennsylvania 19090.
McMullin
M
M
Hardy
D
D
Rieders
F
F
D001826:Body Fluids / Q000032:analysis*; D002849:Chromatography, Gas; D003500:Cyclization; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009584:Nitrogen / Q000032:analysis; D010422:Pentanones; D010624:Phenelzine / Q000032:analysis* / Q000097:blood; D012997:Solvents
D010422:Pentanones; D012997:Solvents; C008790:acetylacetone; D009584:Nitrogen; D010624:Phenelzine