Evidence for protein-catalyzed transfer of platelet activating factor by macrophage cytosol.
Platelet activating factor (PAF) is a potent, proinflammatory lipid. PAF is synthesized in response to stimuli and is rapidly destroyed by specific acetylhydrolases. In order to express its biological activity, PAF and its metabolites are transported among subcellular membranes by as yet unexplained mechanisms. We report here an assay system using methylcarbamyl-PAF (CPAF, 1-O-hexadecyl-2-O-(N-methylcarbamyl)-sn-glycero-3-phosphocholine) and a vesicle-extrusion technique for examining protein-catalyzed intermembrane transfer of CPAF, and demonstrate the presence of proteins catalyzing the separate transfer of CPAF and diacyl phosphatidylcholine in macrophage cytosol. The CPAF transfer activity is heat- and trypsin-sensitive and elutes from gel-filtration columns well separated from proteins catalyzing the transfer of phosphatidylcholine.
Banks
J B
JB
Biochemistry Group, Western Carolina University, Cullowhee, NC 28723.
Wykle
R L
RL
O'Flaherty
J T
JT
Lumb
R H
RH
D000818:Animals; D001692:Biological Transport; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism*; D009928:Organ Specificity; D010742:Phospholipid Ethers / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits
D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; D011506:Proteins; C053479:1-O-hexadecyl-2-N-methylcarbamylphosphatidylcholine