The distinction of bone and liver isoenzymes of alkaline phosphatase in serum using a monoclonal antibody.
Out of 31 mouse monoclonal antibodies to human liver and bone ALP, one antibody coded BAP 1/9, showing a preference for binding the bone isoenzyme, and one antibody coded LAP 1/5, showing no preference, were selected to study binding of the serum forms of ALP. Using a plate capture assay format BAP 1/9 showed a 1.8-fold preference for binding purified bone versus liver ALP in the presence of 10% ALP-free serum compared with 2.1- to 2.25-fold preference in buffered salt solution. BAP 1/9 showed a preference for binding ALP from serum samples containing predominantly bone ALP compared to those containing predominantly liver ALP. Using predefined mixtures of serum forms of bone and liver ALP a linear relationship between ALP bound to BAP 1/9 and the fraction of bone ALP was obtained.
Seabrook
R N
RN
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.
Bailyes
E M
EM
Price
C P
CP
Siddle
K
K
Luzio
J P
JP
D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D000911:Antibodies, Monoclonal; D001842:Bone and Bones / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008099:Liver / Q000201:enzymology*; D051379:Mice
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D000469:Alkaline Phosphatase