[Inactivation of O6-alkylguanine-DNA alkyltransferase in the liver of rats during administration of methylated DNA].
Intraperitoneal administration of 6.5 mg of methylated DNA, O6-methylguanine concentration being 11.5% that of guanine, to 3 month-old noninbred male rats was followed by a 5-fold decrease in the activity of O6-alkylguanine-DNA alkyltransferase (AT) within the first hour. The said enzyme is responsible for DNA O6-alkylguanine repair and plays a key role in mutagenic, carcinogenic and cytostatic effects of some alkylating agents. Within 24 hours, AT activity returned to normal to be followed by a significant increase. A correlation between the degree of inhibition of AT activity and dose of methylated DNA was established. Untreated DNA did not produce an inhibitory effect.
Likhachev
A Ia
AIa
Anisimov
V N
VN
Zhukovskaia
N V
NV
Petrov
A S
AS
D000818:Animals; D004247:DNA / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000201:enzymology*; D008297:Male; D008780:Methyltransferases / Q000037:antagonists & inhibitors*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D051381:Rats
D004247:DNA; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase
Inaktivirovanie O6-alkilguanin-DNK alkiltransferazy v pecheni krys pri vvedenii metilirovannoĭ DNK.