title: Isolation and partial characterization of neutrophil elastase inhibitors from bovine vitreous and aorta.
abstract: Bovine vitreous body and aorta contain extractable leukocyte elastase inhibitors, which were purified by gel filtration and affinity chromatography on agarose-pancreatic elastase. The purified inhibitor preparation from aorta was resolved by polyacrylamide gel electrophoresis into a main band migrating slightly faster than commercial Trasylol and a more weakly stained band migrating close to chymotrypsinogen. The purified inhibitor preparation from both sources inhibited, in a competitive fashion, purified human leukocyte elastase and was ineffective against bovine trypsin and leukocyte cathepsin G or collagenase. These inhibitors from vitreous body and aorta were distinguishable by several criteria from serum inhibitors.
authors: Department of Biochemistry, Rush Medical College Chicago, IL 60612.
authors: C
authors: C
authors: Arsenis
authors: K E
authors: KE
authors: Kuettner
authors: R
authors: R
authors: Eisenstein
mesh_terms: D000818:Animals; D001011:Aorta / Q000032:analysis*; D002417:Cattle; D007962:Leukocytes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D011506:Proteins / Q000302:isolation & purification*; D015843:Serpins; D014822:Vitreous Body / Q000032:analysis*
chemical_list: D011506:Proteins; D015843:Serpins; D010196:Pancreatic Elastase