title: Role of spectrin in cross bonding of the red cell membrane.
abstract: Membrane cross bonding--an adhesion between opposing areas of the cytoplasmic face of the red cell membrane--was achieved by treating red cells with heat, diamide, N-ethymaleimide, urea, or by ATP depletion in conjunction with cell shrinking. Membrane cross bonding could be recognized by the shape of the cells upon swelling. Quantitated by the percentage of cross-bonded red cells the effectivity of the treatments decreased in the order given above. Cross bonding was hardly reversible by reducing the diamide-induced S-S bonds with dithioerythritol. The effect of heat and urea treatment as well as ATP depletion was partly reversible. Transmission electron micrographs of the cross-bonded region showed basically parallel membranes. The distance between the respective phospholipid bilayers varied between 40 and 120 nm from cell to cell. Hb-free ghosts prepared from diamide-treated red cells could also be cross bonded. The following conclusions are drawn: spectrin provides the molecular cross link in membrane cross bonding. Aggregation and enrichment of spectrin in the cross-bonded region are probably involved in membrane cross bonding.
authors: Abt. Physiologie, Medizinische Fakultät, Rheinisch-Westfälische Technische Hochschule, Aachen, FRG.
authors: T M
authors: TM
authors: Fischer
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001952:Bridged-Ring Compounds; D004910:Erythrocyte Membrane / Q000502:physiology* / Q000648:ultrastructure; D006358:Hot Temperature; D006801:Humans; D008854:Microscopy, Electron; D011489:Protein Denaturation; D013049:Spectrin / Q000502:physiology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
chemical_list: D001952:Bridged-Ring Compounds; D013438:Sulfhydryl Compounds; D013049:Spectrin; D000255:Adenosine Triphosphate; D014508:Urea