Uptake and processing of [3H]retinoids in rat liver studied by electron microscopic autoradiography.
The role of rat liver cell organelles in retinoid uptake and processing was studied by electron microscopic autoradiography. [3H]Retinoids were administered either orally, to make an inventory of the cell organelles involved, or intravenously as chylomicron remnant constituents to study retinoid processing by the liver with time. No qualitative differences were observed between the two routes of administration. Time-related changes in the distribution of grains were studied using chylomicron remnant [3H]retinoids. The percentages of grains observed over cells and the space of Disse at 5 and 30 min after administration were, respectively: parenchymal cells, 72.6 and 70.4%; fat-storing cells, 5.0 and 18.1%, and the space of Disse, 14.4 and 8.9%. Low numbers of grains were observed over endothelial and Kupffer cells. The percentages of grains observed over parenchymal cell organelles were, respectively: sinusoidal area, 59.6 and 34.4%; smooth endoplasmic reticulum associated with glycogen, 13.8 and 13.4%; mitochondria, 5.4 and 13.6%; rough endoplasmic reticulum, 4.2 and 7.3%, and rough endoplasmic reticulum associated with mitochondria, 3.7 and 6.5%. It is concluded that chylomicron remnant [3H]retinoids in combination with electron microscopic autoradiography provide a good system to study the liver processing of retinoids in vivo. These results, obtained in the intact liver under physiological conditions, further substantiate that retinoids are processed through parenchymal cells before storage occurs in fat-storing cell lipid droplets, that retinoid uptake is not mediated through lysosomes and that the endoplasmic reticulum is a major organelle in retinoid processing.
Hendriks
H F
HF
TNO Institute for Experimental Gerontology, HV Rijswijk, The Netherlands.
Elhanany
E
E
Brouwer
A
A
de Leeuw
A M
AM
Knook
D L
DL
D000284:Administration, Oral; D000818:Animals; D001345:Autoradiography; D002914:Chylomicrons / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012176:Retinoids / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D014316:Tritium
D002914:Chylomicrons; D012176:Retinoids; D014316:Tritium