[Effect of the synthetic endogenous opioid analog dalargin on the natural cytotoxicity of human lymphocytes].
The effect of synthetic opioid peptide analogue dalargin on the natural cytotoxicity of lymphocytes from the peripheral human blood has been studied in respect to target cells K-562. Natural cytotoxicity was determined by assessing the cytotoxicity of individual effector cells in agarose. It has been shown that preincubation of lymphocytes with 10(-14)--10(-8) M of dalargin enhances the binding of NK-cells with targets and the formation of conjugates with killed targets and increases the percentage of active NK-cells. In some cases bimodal kinetics of a stimulating dalargin effect on the natural cytotoxicity has been observed, with two peaks of the peptide activity being in the range of 10(14)--10(-12) and 10(-10)--10(-6) M. Naloxone at a concentration of 10(-6) M acted as an agonist of opioid receptors, enhancing the formation of effector-target conjugates and NK-cell lysis abilities. A stimulating effect of dalargin on the natural cytotoxicity was more marked than that of recombinant human alpha-interferon. The possible use of dalargin in pathological conditions characterized by disturbed NK-cell function is discussed.
Aleksandrova
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EN
Nasonov
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EL
Vinogradov
V A
VA
Titov
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MI
D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*
D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C040996:enkephalin-Leu, Ala(2)-Arg(6)-
Vliianie sinteticheskogo analoga éndogennykh opioidov dalargina na estestvennuiu tsitotoksichnost' limfotsitov cheloveka.