title: Apical sodium-sugar transport in pulmonary epithelium in situ.
abstract: The presence of an apical sodium-coupled transport system for D-glucose in lung alveolar epithelial cells has been demonstrated in lungs instilled with Ringer's fluid and perfused with either blood or Ringer's fluid (Basset et al. (1987) J. Physiol. 384, 325-345). The direction of transport is from alveoli towards interstitium. The characteristics of the system were evaluated in similar preparations by use of sugar analogues such as alpha-methyl-glucopyranoside, 2-deoxyglucose, 3-O-methylglucose and L-glucose. The main finding was the presence of a transport system for alpha-methylglucopyranoside and 2-deoxyglucose in the apical cell membrane. This system was unaffected by phloretin. Both alpha-methylglucopyranoside and 2-deoxyglucose transports were inhibited by phloridzin and by the presence of glucose (10(-2) mol.l-1). Competition was demonstrated between D-glucose and alpha-methylglucopyranoside or 2-deoxyglucose, but not for 3-O-methylglucose or L-glucose. 3-O-Methylglucose was cleared as slowly as L-glucose. The results comply partly with those known from intestinal epithelium and kidney proximal tubular epithelium, but the handling of 3-O-methylglucose was different. The relative transport rates of Na+ and glucose are compatible with a Na+: glucose coupling ratio larger than one.
authors: INSERM U82, Faculté Xavier Bichat, Paris, France.
authors: G
authors: G
authors: Basset
authors: G
authors: G
authors: Saumon
authors: F
authors: F
authors: Bouchonnet
authors: C
authors: C
authors: Crone
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D004848:Epithelium / Q000378:metabolism; D005947:Glucose / Q000031:analogs & derivatives / Q000378:metabolism*; D008564:Membrane Potentials; D010227:Parabiosis; D010477:Perfusion; D011650:Pulmonary Alveoli / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism*
chemical_list: D012964:Sodium; D005947:Glucose