Midazolam in plasma from hospitalized patients as measured by gas-liquid chromatography with electron-capture detection.
The present assay was developed for quantifying midazolam in plasma of patients hospitalized in intensive-care units or undergoing anesthesia and receiving many other drugs as well. Plasma samples are alkalinized with NaOH and midazolam is extracted into n-hexane. The organic phase is evaporated and reconstituted in n-butyl acetate, and the midazolam is quantified by gas-liquid chromatography with electron-capture detection. The calibration graph for midazolam was linear in the ranges 5-200 and 200-800 micrograms/L. The CVs for precision and reproducibility of the assay were less than 8%. The method was very specific for midazolam; most of the drugs commonly used in anesthesia and in the intensive-care unit did not interfere with the assay. The lowest detectable concentration was 1 microgram/L. The method is adaptable for use with an automated chromatographic system.
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HR
Department of Anesthesia, University of Basle/Kantonsspital, Switzerland.
Funk
B
B
Maitre
P O
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Zbinden
A M
AM
Follath
F
F
Thomson
D A
DA
D000368:Aged; D000758:Anesthesia; D002849:Chromatography, Gas; D006760:Hospitalization; D006801:Humans; D007362:Intensive Care Units; D008874:Midazolam / Q000097:blood*
D008874:Midazolam