Dopamine inhibits calcium flux in the 7315a prolactin-secreting pituitary tumour.
Cells of the 7315a prolactin-secreting tumour express biochemically normal cell-surface receptors for dopamine. However, dopamine inhibits prolactin release from these cells only when the basal rate of prolactin release is augmented by increasing the intracellular and/or extracellular calcium concentration of the tumour cells. This suggests that dopaminergic modulation of calcium ion flux could have a central physiological role in these neoplastic cells. In 7315a cells we examined the ability of dopamine to regulate 45Ca2+ influx and fractional 45Ca2+ efflux under conditions of enhanced calcium flux using the calcium channel activator, maitotoxin. It was observed that unidirectional calcium influx stimulated by maitotoxin was significantly inhibited by dopamine. Maitotoxin stimulated fractional efflux and prolactin release from the tumour cells and dopamine simultaneously inhibited both processes by a haloperidol-reversible mechanism. Therefore, in 7315a cells dopamine receptor activation is coupled to inhibition of calcium flux as at least one component in the regulation of prolactin release. These cells may provide further opportunity to study intracellular signalling mechanisms that are modulated by dopamine receptor activity.
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IS
Department of Neurology, University of Virginia School of Medicine, Charlottesville.
Judd
A M
AM
Macleod
R M
RM
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004298:Dopamine / Q000494:pharmacology*; D008387:Marine Toxins / Q000494:pharmacology*; D035964:Oxocins; D010911:Pituitary Neoplasms / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D000095702:Polyether Toxins
D002118:Calcium; D004298:Dopamine; D008387:Marine Toxins; D035964:Oxocins; D011388:Prolactin; C020394:maitotoxin; D000095702:Polyether Toxins