In vivo and solid state 13C nuclear magnetic resonance studies of tyrosine metabolism during insect cuticle formation.
1. Tyrosine metabolism during pupation can be followed in living Heliothis virescens larvae using nuclear magnetic resonance spectroscopy. 2. Loss of 13C signals from a label at the C-3 position of tyrosine during pupation indicates uptake of tyrosine into solid cuticle. 3. Solids 13C NMR spectroscopy of cuticle formed by insects injected with 3-13C labelled tyrosine indicates that little change in chemical shift occurs during cuticle hardening, indicating that the side chain is probably not involved in protein cross-linking.
Williams
H J
HJ
Department of Chemistry, Texas A&M University, College Station 77843.
Scott
A I
AI
Woolfenden
W R
WR
Grant
D M
DM
Vinison
S B
SB
Elzen
G W
GW
Baehrecke
E H
EH
D000818:Animals; D007814:Larva / Q000378:metabolism; D007915:Lepidoptera / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D012016:Reference Values; D014443:Tyrosine / Q000378:metabolism*
D014443:Tyrosine