title: Ultrastructure of mucous blanket in otitis media with effusion.
abstract: We used transmission electron microscopy to study the mucous blanket of the promontory from children with otitis media with effusion. The vast majority of the epithelial cells were secretory, and the rest were ciliated. The mucous blanket consisted of the electron-lucent periciliary fluid and the mucous layer. In the mucous layer, two layers were identified: an inner layer with migrating cells, and an outer layer with specks. Moreover, there was a lucent zone over the nonciliated surface that was as high as the microvilli. The thickness of the periciliary layer was predominantly as great as that of the ciliary tips, which just make contact with the mucous layer; however, the mucous layer occasionally penetrated into the periciliary space. These findings indicated that there is a mucociliary dysfunction in the middle ear caused by a decrease in the number of ciliated cells, and an abnormal interaction between cilia and mucus that would interfere with ciliary movement. Thus, such a system would fail to transport the mucous blanket.
authors: Department of Otorhinolarygology, Mie University School of Medicine, Tsu, Japan.
authors: M
authors: M
authors: Inagaki
authors: Y
authors: Y
authors: Sakakura
authors: T
authors: T
authors: Shimizu
authors: Y
authors: Y
authors: Majima
authors: K
authors: K
authors: Ukai
mesh_terms: D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D002923:Cilia / Q000648:ultrastructure; D002925:Ciliary Motility Disorders / Q000473:pathology; D004432:Ear, Middle / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D009092:Mucous Membrane / Q000648:ultrastructure; D009093:Mucus / Q000166:cytology; D010034:Otitis Media with Effusion / Q000473:pathology*