The N- and O-linked carbohydrate chains of human, bovine and porcine plasminogen. Species specificity in relation to sialylation and fucosylation patterns.
The structures of the N- and O-glycans of human, bovine and porcine plasminogen were determined by 500-MHz 1H-NMR spectroscopy. The N-glycans of all three species proved to be of the N-acetyllactosamine type differing from one another with respect to the sialylation and fucosylation patterns. In the N-glycan of human plasminogen the two antennae are sialylated with N-acetylneuraminic acid (NeuAc), whereas in the bovine counterpart both branches carry significant amounts of N-glycolylneuraminic acid (NeuGc). In porcine plasminogen the sialic acid is mainly NeuAc; the Man alpha 1----6 branch, however, is only partially sialylated. In addition, the porcine N-glycan is fucosylated to about 80% in alpha 1----6 linkage to the GlcNAc-1 residue. The O-glycans of the three species possess an identical Gal beta 1----3GalNAc core which is alpha 2----3 sialylated with NeuAc at Gal. The disialylated form, which is also present in all three species, has an additional NeuAc residue in alpha 2----6 linkage to GalNAc. Mono- and disialylated forms occur in different molar ratios in the different plasminogens: 80:20 in human, 70:30 in bovine and 50:50 in porcine. This study on the carbohydrate moiety of these three plasminogens reveals species specificity in terms of various types of microheterogeneities.
Marti
T
T
Institute of Biochemistry, University of Bern, Switzerland.
Schaller
J
J
Rickli
E E
EE
Schmid
K
K
Kamerling
J P
JP
Gerwig
G J
GJ
van Halbeek
H
H
Vliegenthart
J F
JF
D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D005643:Fucose / Q000378:metabolism*; D006801:Humans; D019158:N-Acetylneuraminic Acid; D009438:Neuraminic Acids; D010958:Plasminogen / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification; D012794:Sialic Acids / Q000378:metabolism*; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013552:Swine
D009438:Neuraminic Acids; D011134:Polysaccharides; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid; D005643:Fucose; D010958:Plasminogen; D019158:N-Acetylneuraminic Acid