Erythrocyte D-glucose transport activity in reconstituted model membranes of different lipid composition.
The stereospecific influx of D-glucose into liposomes formed on sonication of different glyco- and phospholipids with transport proteins from human erythrocyte ghosts solubilized with Triton x-100 was measured as an index of their total D-glucose transport activity. Specific D-glucose transport increased when acidic phospho- and glycolipids (especially sulfatide) were added to the phosphatidylcholine bilayers of the model membranes while cholesterol strongly inhibited the process. The modulation of D-glucose transport activity and its possible correlation with the lipid composition and the chemico-physical state of the erythrocytes is discussed.
Cestaro
B
B
Dipartimento di Chimica e Biochimica Medica, Facoltà di Medicina e Chirurgia, Milano, Italy.
Cervato
G
G
Carandente
O
O
Girardi
A M
AM
Pozza
G
G
D002554:Cerebrosides / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D004912:Erythrocytes / Q000378:metabolism*; D005732:Gangliosides / Q000032:analysis; D005947:Glucose / Q000493:pharmacokinetics*; D008081:Liposomes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D010713:Phosphatidylcholines / Q000032:analysis; D013433:Sulfoglycosphingolipids / Q000032:analysis
D002554:Cerebrosides; D005732:Gangliosides; D008081:Liposomes; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D013433:Sulfoglycosphingolipids; D002784:Cholesterol; D005947:Glucose