[Isolation and properties of genetic engineered human leukocyte interferons alphaI1 and alphaN].
Using controlled pore glass chromatography and immunoaffinity chromatography on monoclonal antibodies NK-2 immobilized on Sepharose 4B, the electrophoretically homogeneous interferons alpha N and alpha I1 were isolated from the biomass of gene-engineered Pseudomonas sp. strains. In terms of specific activity on human fibroblast diploid cells, interferon alpha I1 does not differ from interferon alpha A, whereas the specific antiviral activity of interferon alpha N is as low as 2.10(7) JU/mg. The procedures for immunometric assay of interferons alpha N and alpha I1 have been elaborated. Various monoclonal antibodies to interferon alpha A and to natural leucocyte interferon were analyzed; among those the antibodies specifically interacting with interferons alpha N and alpha I1 (but not with interferon alpha A) were identified.
Eremashvili
M R
MR
Borukhov
S I
SI
Bakhutashvili
V I
VI
Naroditskaia
V A
VA
Chistoserdov
A Iu
AIu
D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007370:Interferon Type I / Q000302:isolation & purification* / Q000494:pharmacology; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D007370:Interferon Type I; D011994:Recombinant Proteins
Vydelenie i svoĭstva genno-inzhenernykh chelovecheskikh leĭkotsitarnykh interferonov alphaI1 i alphaN.