Endogenous immunoreactive glia maturation factor-like molecule in cultured rat Schwann cells.
Using the monoclonal antibody G2-09 raised against bovine glia maturation factor (GMF), we demonstrated that cultured rat Schwann cells and Schwannoma cells, but not their conditioned media, possessed endogenous GMF-like immunoreactivity. The presence of immunoreactive GMF correlated well with GMF bioactivity. The GMF-like factor in Schwann cells was characterized by immunodotting, immunofluorescence, immunoadsorption and immunoblotting. Immunofluorescence confirmed the intracellular location of GMF. Immunoadsorption completely eliminated the GMF-like bioactivity from the cell extracts. Immunoblotting identified a protein band with a molecular weight of 14,000. Thus, the evidence strongly supports the argument that the GMF-like factor in rat Schwann cells is identical with GMF from the bovine brain. The GMF-like molecule in Schwannoma cells showed properties similar to those in Schwann cells, but for unknown reasons was not detectable by immunofluorescence. The presence of GMF in cultured rat Schwann cells suggests that the factor may play a role in the peripheral nervous system.
Lim
R
R
Department of Neurology (Division of Neurochemistry and Neurobiology), University of Iowa College of Medicine, Iowa City.
Hicklin
D J
DJ
Ryken
T C
TC
Miller
J F
JF
Bosch
E P
EP
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D020904:Glia Maturation Factor; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009442:Neurilemmoma; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D020904:Glia Maturation Factor; D009419:Nerve Tissue Proteins