Metabolism of the Z and E isomers of N-nitroso-N-methyl-(2-oxopropyl)amine by rat hepatocytes.
The metabolism of N-nitroso-N-methyl-N-(2-oxopropyl)amine was examined using freshly isolated hepatocytes from Fischer 344 rats. As determined by high performance liquid chromatography, it was found that the E isomer was preferentially metabolized when the parent mixture was used. When the two isomers were studied separately, the E isomer was efficiently metabolized in the hepatocytic system, whereas the Z isomer was not. The kinetics of disappearance of the Z isomer during metabolism was identical to that for the reequilibration of the Z isomer to the mixture of isomers in the absence of a metabolizing system.
Farrelly
J G
JG
NCI-Frederick Cancer Research Facility, Basic Research Program, Maryland 21701.
Stewart
M L
ML
Farnsworth
D W
DW
Saavedra
J E
JE
D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism*; D051381:Rats; D013237:Stereoisomerism
D002273:Carcinogens; D009602:Nitrosamines; C028133:N-nitrosomethyl-2-oxopropylamine