MP17, a fiber-specific intrinsic membrane protein from mammalian eye lens.
A major protein with a molecular weight of 17,000, designated as MP17, has been identified in mammalian eye lens plasma membranes. Hydrophobic photolabeling experiments revealed that MP17 is a genuine intrinsic membrane protein. By using monoclonal antibodies we demonstrated that MP17 is not detectable in liver, heart, muscle, spleen and kidney, and thus can be considered, like MP26, as a lens-specific membrane protein. Furthermore, we showed that MP17 is a substrate for cAMP-dependent protein kinase and that it is a calmodulin-binding protein.
Mulders
J W
JW
Department of Biochemistry, University of Nijmegen, The Netherlands.
Voorter
C E
CE
Lamers
C
C
de Haard-Hoekman
W A
WA
Montecucco
C
C
van de Ven
W J
WJ
Bloemendal
H
H
de Jong
W W
WW
D000818:Animals; D000911:Antibodies, Monoclonal; D002147:Calmodulin / Q000378:metabolism; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003459:Crystallins / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D002147:Calmodulin; D003459:Crystallins; D005136:Eye Proteins; D008565:Membrane Proteins; C055948:lens intrinsic protein MP 17; D011494:Protein Kinases