Resistance of KP and CBA mice to cadmium chloride assessed on the basis of the bone marrow stromal cell growth in vitro.
Using a culture of bone marrow stromal cells, resistance of two inbred mice strains, KP and CBA, to cadmium chloride was assessed. Mice were administered with a single dose of CdCl2 (12 mumoles (kg b. wt) and bone marrow cells were examined after 7 days of culture. In KP strain, a decrease in the number of fibroblasts and proliferation of adipocytes was observed, as compared with control animals. In CBA strain, cadmium did not influence the number of fibroblasts and caused only an insignificant increase in the amount of adipocytes. Red blood cell count, hematocrit and Fe2+ level were unchanged both strains after cadmium administration. The observed differences in populations of cultured stromal cells confirmed the sensitivity of the KP mice to cadmium and indicated that the stromal cells can be regarded as useful indicator of cadmium intoxication.
Godowicz
B
B
Department of Genetics and Evolution, Jagellonian University, Cracow, Poland.
Miszta
H
H
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001854:Bone Marrow Cells; D002104:Cadmium / Q000494:pharmacology* / Q000633:toxicity; D019256:Cadmium Chloride; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004351:Drug Resistance; D005347:Fibroblasts / Q000166:cytology; D008264:Macrophages / Q000166:cytology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains
D002104:Cadmium; D019256:Cadmium Chloride