Reversal of the metastatic phenotype in Lewis lung carcinoma cells after transfection with syngeneic H-2Kb gene.
High metastatic clones of the murine 3LL carcinoma express greatly reduced levels of H-2Kb major histocompatibility complex class I antigens, while low metastatic clones of the same tumor express high levels of H-2Kb. Induced expression of this antigen after transfection with the H-2Kb gene resulted in conversion of a metastatic to a non- or low-metastatic phenotype. Unlike the parental cells, transfected cells are potent inducers of H-2Kb-restricted syngeneic cytotoxic lymphocytes that kill the Kb-positive clones and cross-react with parental nontransfected cells. Preimmunization of mice with Kb-positive transfectants conferred protection against metastatic spread of malignant cells. Moreover, immunotherapy of metastasis was achieved by immunization with the H-2Kb-transfected cells of animals already carrying a growing local tumor of the parental cells.
Plaksin
D
D
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Gelber
C
C
Feldman
M
M
Eisenbach
L
L
D000818:Animals; D005805:Genes, MHC Class I; D006183:H-2 Antigens / Q000235:genetics*; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids; D014158:Transcription, Genetic; D014162:Transfection; D014185:Transplantation, Isogeneic
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse