Characteristics of 125I-Bolton-Hunter labelled cholecystokinin binding in human brain.
The high affinity binding of 125I-Bolton-Hunter labelled CCK-8 (125I-BHCCK) was studied in human brain using ligand binding and autoradiographic techniques. High levels of 125I-BHCCK binding were observed in cortex, striatum and cerebellum. In cerebellar membranes 125I-BHCCK binding was inhibited by CCK analogues with an order of potency, caerulein greater than CCK-8 greater than CCK-33 greater than gastrin 1-17 = CCK-8 non-sulphated. CCK-4 was inactive. It is suggested that in human brain 125I-BHCCK labels a population of CCK receptors similar to those observed in guinea pig brain. The properties and distribution of these receptors differ from those of rat brain.
Cross
A J
AJ
Department of Physiology, University of Manchester.
Slater
P
P
Skan
W
W
D000368:Aged; D001921:Brain / Q000378:metabolism*; D002766:Cholecystokinin / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D011949:Receptors, Cholecystokinin / Q000378:metabolism; D012844:Sincalide / Q000031:analogs & derivatives / Q000378:metabolism; D013388:Succinimides / Q000378:metabolism; D014018:Tissue Distribution
C044758:Bolton Hunter-cholecystokinin octapeptide; D011949:Receptors, Cholecystokinin; D013388:Succinimides; D002766:Cholecystokinin; D012844:Sincalide