Synthesis of extracellular matrix glycoproteins by a differentiated thyroid epithelial cell line.
We examined the synthesis of extracellular matrix macromolecules by the differentiated rat thyroid epithelial cell line FRTL-5. As shown by electron microscopy, the extracellular material produced by these cells is deposited at the basolateral surface and focally organized in the form of a basement membrane. Biochemical and biosynthetic studies demonstrated that laminin, type IV collagen, and fibronectin are synthesized and deposited in the culture monolayer. Secretion of fibronectin into the culture medium also occurred. By immunofluorescence we observed some peculiarities in the distribution patterns of the basement membrane glycoproteins; while fibronectin and laminin had an almost superimposable distribution, type IV collagen displayed a rather different pattern. Type IV collagen and laminin localization at sites where extracellular material was detected was confirmed by immuno electronmicroscopy using the protein A-colloidal gold technique. The results indicate that under appropriate culture conditions the differentiated thyroid epithelial cell line FRTL-5 synthesizes, secretes and organizes an extracellular matrix where some basement membrane glycoproteins are present.
Garbi
C
C
Centro di Endocrinologia ed Oncologia Sperimentale del CNR, II Facoltà di Medicina, Napoli, Italy.
Zurzolo
C
C
Bifulco
M
M
Nitsch
L
L
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D005353:Fibronectins / Q000096:biosynthesis; D007797:Laminin / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008854:Microscopy, Electron; D013961:Thyroid Gland / Q000378:metabolism*
D005353:Fibronectins; D007797:Laminin; D008562:Membrane Glycoproteins; D003094:Collagen