In vitro synthesis of infectious retroviral RNA.
A plasmid was constructed in which a T7 RNA polymerase promoter was placed upstream of a recombinant amphotropic retrovirus genome containing a selectable neomycin resistance gene. To test the infectivity of the RNA produced by T7 RNA polymerase in vitro, the RNA was microinjected into the nuclei of psi 2 packaging cells. Infectious particles conferring G418 resistance were released.
Flamant
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Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.
Sorge
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JA
D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D008845:Microinjections; D009355:Neomycin / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000096:biosynthesis*; D012190:Retroviridae / Q000235:genetics*; D014771:Virion / Q000032:analysis
D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D009355:Neomycin