Accumulation of platelets and eosinophils in baboon lung after paf-acether challenge. Inhibition by ketotifen.
Intratracheal administration of platelet-activating factor (paf-acether) induced transient bronchoconstriction in baboons. Using an automated isotopic monitoring system, we found that intratracheal administration of paf-acether also elicited transient accumulation of platelets labeled with 111In oxine within the pulmonary vasculature after the increase in maximal peak inspiratory pressure. Bronchoalveolar eosinophilia were inhibited by prophylactic administration of the antiasthma drug ketotifen but not by pyribenzamine, suggesting that the effects of ketotifen are unrelated to H-1 receptor antagonism. Platelet accumulation was not affected by ketotifen or pyribenzamine. This study suggests that paf-acether may be a mediator of the eosinophil recruitment in bronchial asthma and that inhibition of this phenomenon by ketotifen may contribute to the therapeutic efficacy of this drug.
Arnoux
B
B
INSERM U 200, Hôpital Antoine Béclére, Clamart, France.
Denjean
A
A
Page
C P
CP
Nolibe
D
D
Morley
J
J
Benveniste
J
J
D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D004804:Eosinophils / Q000166:cytology*; D007665:Ketotifen / Q000494:pharmacology*; D008168:Lung / Q000166:cytology* / Q000502:physiology; D010215:Papio; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011312:Pressure; D012119:Respiration
D010972:Platelet Activating Factor; D007665:Ketotifen