Serum amino acid levels in leukemic mice after L-asparaginase treatment.
A rapid, reproducible HPLC method based on dansyl chloride derivatization has been developed for the determination of L-asparagine, L-aspartate, L-glutamine, and L-glutamate in mouse and human serum samples. This improved procedure has been designed for automation with an autoinjector system. Studies with mice bearing the sensitive and the asparaginase-resistant L5178Y leukemia show that this analytical method can be employed to monitor the effect of L-asparaginase on serum levels of these four amino acids. The method can be used to monitor serum amino acid levels in patients undergoing therapy with L-asparaginase.
Woodward
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Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103.
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Capizzi
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Modest
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EJ
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D001215:Asparaginase / Q000627:therapeutic use*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003619:Dansyl Compounds / Q000032:analysis; D007942:Leukemia, Experimental / Q000097:blood* / Q000188:drug therapy; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
D000596:Amino Acids; D003619:Dansyl Compounds; D001215:Asparaginase