Differential expression of the cytochrome P-450c and P-450d genes in the rat ventral prostate and liver.
Complementary DNA clones specific for cytochromes P-450c and P-450d were used to study the expression of corresponding mRNAs in the rat ventral prostate. Following treatment with beta-naphthoflavone (BNF), an increase in total cellular levels of cytochrome P-450c mRNA was observed in this tissue. The induction kinetics were similar in both rat liver and prostate with regard to the expression of the cytochrome P-450c gene. The mRNA levels reached a maximum at 16 h and decreased to control levels at about 48 h. In contrast, mRNA specific for cytochrome P-450d was detectable only in the liver following BNF treatment (maximum at 24 h). The tissue-specific expression of cytochromes P-450c and P-450d was further investigated using measurements of nuclear transcription in vitro. RNA transcripts specific for cytochrome P-450c were detected in nuclei from both liver and prostate following BNF induction. The rate of cytochrome P-450c transcription was maximal at 4 h and 8-12 h in the liver and prostate, respectively. In the liver, induction by BNF of the rate of transcription of the cytochrome P-450d gene occurred at a slightly later time point as compared to cytochrome P-450c gene expression. No elongation of RNA specific for cytochrome P-450d could be detected in nuclei from the ventral prostate indicating a transcriptional control of cytochrome P-450d gene expression in this tissue.
Söderkvist
P
P
Department of Medical Nutrition, Karolinska Institutet, Huddinge, Sweden.
Poellinger
L
L
Toftgård
R
R
Gustafsson
J A
JA
D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D005796:Genes; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D010957:Plasmids; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D012333:RNA, Messenger; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System