Metabolism of mono-(2-ethylhexyl)phthalate in fetal, neonatal and adult rat liver.
The aim of the investigation was to study to what extent maturity influences the metabolism of mono-(2-ethylhexyl)phthalate (MEHP), the primary metabolite of the plasticizer di-(2-ethylhexyl)phthalate. The conversion of MEHP to its (omega-1)-hydroxylated product was determined in liver microsomes from fetal, neonatal (1- and 5-day-old) and adult rats. Product formation was determined by gas chromatography-mass spectrometry. The results show that fetal and neonatal as well as adult rat livers are capable of metabolizing MEHP by (omega-1)-hydroxylation. Preparations from 1- and 5-day-old rats were much more efficient than those from fetal rats. The transition into adult life gave no further increase in hydroxylase activity as compared to that in 5-day-old rats. The work shows that there is a rapid postnatal development of MEHP (omega-1)-hydroxylase activity in the rat.
Sjöberg
P
P
Department of Drugs, National Board of Health and Welfare, Uppsala, Sweden.
Bondesson
U
U
Gustafsson
J
J
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D042926:Cytochrome P-450 CYP4A; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives / Q000378:metabolism* / Q000493:pharmacokinetics; D005260:Female; D005333:Fetus / Q000201:enzymology*; D008401:Gas Chromatography-Mass Spectrometry; D008658:Inactivation, Metabolic; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010795:Phthalic Acids / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D010795:Phthalic Acids; D004051:Diethylhexyl Phthalate; D006899:Mixed Function Oxygenases; D042926:Cytochrome P-450 CYP4A; C016599:mono-(2-ethylhexyl)phthalate