Method for stable intracellular recordings of spinal alpha-motoneurons during treadmill walking in awake, intact cats.
A method is described for recording from spinal alpha-motoneurons, which are identified according to muscle innervated, with an intracellular microelectrode during treadmill walking in awake, normally respiring, intact, adult cats. The procedure involves the implantation of an orthopedic-style spinal unit, the training of the subject to locomote on the treadmill with the lumbar region restrained, and the micropipette recording of motoneurons through a small laminotomy.
Glenn
L L
LL
Department of Physiology, Ohio College of Podiatric Medicine, Cleveland 44106-3082.
Whitney
J F
JF
Rewitzer
J S
JS
Salamone
J A
JA
Mariash
S A
SA
D000818:Animals; D002415:Cats; D005260:Female; D008297:Male; D008564:Membrane Potentials; D009046:Motor Neurons / Q000502:physiology*; D009132:Muscles / Q000502:physiology; D005082:Physical Exertion; D012016:Reference Values; D013116:Spinal Cord / Q000502:physiology*