title: Chemical-induced modulation of ATP and protein synthesis processed inside rat brain mitochondria.
abstract: Rates of ATP production and protein synthesis rates were simultaneously studied using the isolated rat brain mitochondria and different types of brain polysomes (total, free, microsomal and mitochondrial membrane-bound). Protein synthesis was measured using a special reticulocyte lysate cell-free incubation medium. Prior to incubation, the intact ("unbroken") mitochondria were pretreated by several xenobiotics and natural metabolic effectors. The mitochondria incubated without these additions were used as controls. Chemically-promoted inhibition of ATP systhesis correlated with a significant activation of protein synthesis directed by the mitochondrial membrane-bound polysomes. The induction of ATP synthesis leads to a marked suppression of mitochondrial translation. Application of the selective translation blockers do not influence the ATP formation inside the isolated mitochondria. In general, mitochondrial membrane-found polysomes isolated from the intact normal organella are least active as compared with other tested polysomal fractions while the releasing of polysomes from mitochondrial membranes by Triton X-100 leads to the essential increase of the activity of these separated translation mechanisms. The separation of polysomes from the microsomal membranes do not change the activity of the former. A possible molecular mechanism, interdependence and biological significance of the established phenomena are discussed.
authors: Laboratory of Toxicology, Moscow City Station for Sanitation and Epidemiology, USSR.
authors: D A
authors: DA
authors: Kuznetsov
authors: P I
authors: PI
authors: Musajev
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002474:Cell-Free System; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D011500:Protein Synthesis Inhibitors; D014475:Uncoupling Agents; D000255:Adenosine Triphosphate