Cell to cell adhesion systems in Xenopus laevis, the South African clawed toad. II: Monoclonal antibody against a novel Ca2+-dependent cell-cell adhesion glycoprotein on amphibian cells.
We isolated a mouse monoclonal antibody that disrupts Ca2+-dependent cell-cell adhesion of amphibian (Xenopus laevis) cells. When added to culture medium, the monoclonal antibody completely disrupted cell-cell adhesion of amphibian cells in monolayer culture and specifically inhibited Ca2+-dependent cell-cell adhesion of dissociated cells in reaggregation experiments. The monoclonal antibody recognized a 140 kDa cell surface glycoprotein antigenically different from the previously reported Ca2+-dependent cell-cell adhesion molecules (cadherins).
Nomura
K
K
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Tajima
T
T
Nomura
H
H
Shiraishi
H
H
Uchida
M
M
Yamana
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002118:Calcium / Q000378:metabolism*; D002448:Cell Adhesion; D002478:Cells, Cultured; D006023:Glycoproteins / Q000378:metabolism*; D006825:Hybridomas / Q000276:immunology; D007120:Immunochemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006023:Glycoproteins; D002118:Calcium