N-methyl-N-nitrosourea-induced and spontaneous AKR/J thymic lymphomas express distinct differentiation antigen phenotypes.
Spontaneous and N-methyl-N-nitrosourea (MNU)-induced AKR/J thymic lymphomas were characterized for expression of several lymphocyte differentiation antigens. The majority (53%) of spontaneous lymphomas expressed both Lyt-2 and L3T4 antigens, similar to the predominant normal thymocyte subset. In contrast, 63% of the thymic lymphomas induced by the chemical carcinogen MNU, expressed an Lyt-2+ L3T4- antigenic profile. Although this profile suggested that MNU-induced lymphomas are phenotypically similar to a mature thymocyte subset, the presence of ThB antigen on Lyt-2+ L3T4- lymphomas did not support this notion. Diagonal gel electrophoresis of 125I-labeled membrane extracts and immunoprecipitates revealed that 17 of 29 Lyt-2+ L3T4-MNU-induced lymphomas expressed cell surface T-cell receptor heterodimer components. Northern blot analyses confirmed that the T-cell receptor material was composed of alpha and beta polypeptide chains. The results from this study indicate a distinct origin or differentiation potential of the target cells involved in viral and chemical induced lymphomagenesis of AKR/J mice.
Richie
E R
ER
University of Texas System Cancer Center, Science Park-Research Division, Smithville 78957.
D000818:Animals; D000943:Antigens, Differentiation / Q000302:isolation & purification*; D000950:Antigens, Ly; D014408:Biomarkers, Tumor / Q000032:analysis; D002472:Cell Transformation, Viral; D046911:Macromolecular Substances; D008770:Methylnitrosourea / Q000494:pharmacology*; D051379:Mice; D008806:Mice, Inbred AKR; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000302:isolation & purification; D012513:Sarcoma, Experimental / Q000139:chemically induced* / Q000145:classification / Q000209:etiology; D013953:Thymus Neoplasms / Q000139:chemically induced* / Q000145:classification / Q000209:etiology
D000943:Antigens, Differentiation; D000950:Antigens, Ly; D014408:Biomarkers, Tumor; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D008770:Methylnitrosourea