Isolation and characterization of growth hormone from blue fox pituitary glands.
Growth hormone has been purified to homogeneity from blue fox pituitary glands. It has 191 amino acids with two disulfide bridges and a single tryptophan residue. The somatotropin activity is only 8% when compared with the bovine hormone in the receptor-binding assay. From radioimmunoassay data using baboon antisera to porcine or bovine growth hormone, the fox hormone has 14-17% immunoreactivity of bovine or porcine hormone.
Li
C H
CH
Laboratory of Molecular Endocrinology, University of California, San Francisco.
Chung
D
D
Izdebski
J
J
Majewski
T
T
Mowles
T F
TF
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000032:analysis; D005589:Foxes / Q000502:physiology*; D013006:Growth Hormone / Q000302:isolation & purification* / Q000378:metabolism; D007106:Immune Sera; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D010215:Papio; D010902:Pituitary Gland / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay; D011986:Receptors, Somatotropin / Q000378:metabolism; D014364:Tryptophan
D004220:Disulfides; D007106:Immune Sera; D011986:Receptors, Somatotropin; D014364:Tryptophan; D013006:Growth Hormone