Tumorigenicity of six clones of a cultured neoplastic cell line derived from a spontaneous murine astrocytoma: morphology and immunocytochemistry of tumours.
Six clones obtained from the neoplastic, astrocytic murine cell line VMDk P497 were injected intracerebrally into syngeneic hosts and the tumorigenicity of each clone was established. Five of the 6 clones produced tumours with incidences ranging from 25% to 100% and mean latencies of 43-100 days, according to the clone injected. Histological, immunocytochemical and electron microscopical examination of the resulting tumours revealed differences in the degree of invasiveness, but otherwise only slight variations in phenotype between the clones. Generally, the tumours were glioblastoma-like, showing a pleomorphic histoarchitectural pattern; the predominant cell types throughout were poorly differentiated, and lacked both antigenic and morphological characteristics, particularly the presence of intermediate filaments, of mature astrocytes. The basal lamina proteins, fibronectin and laminin, were, however, expressed in all tumours examined. This phenotypic change on cloning and syngeneic transplantation may be of considerable significance in future therapeutic studies using this glioma model.
Koppel
H
H
Department of Neuropathology, Institute of Psychiatry, Denmark Hill, London, U.K.
Pilkington
G J
GJ
Lantos
P L
PL
D000818:Animals; D001254:Astrocytoma / Q000032:analysis / Q000473:pathology*; D001932:Brain Neoplasms / Q000032:analysis / Q000209:etiology* / Q000473:pathology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005260:Female; D005353:Fibronectins / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009479:Neuropeptides / Q000032:analysis; D013997:Time Factors
D005353:Fibronectins; D005904:Glial Fibrillary Acidic Protein; D009479:Neuropeptides