New plate medium for screening and presumptive identification of gram-negative urinary tract pathogens.
A new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is described. The medium combines adonitol fermentation, phenylalanine deaminase, and beta-glucuronidase tests and allows the indole and cytochrome oxidase tests to be performed directly from the plates. High-level agreement with individual conventional tests was recorded in comparative studies with 504 cultures of gram-negative rods. There was 100% agreement, except for the Providencia spp. indole spot test (61.6% agreement). Adonitol fermentation by Providencia species could not be determined. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa were identified with a high efficiency (100, 85.7, 83.5, and 100% agreement, respectively) without further testing. There was 96% overall agreement for the 267 infected urine samples tested.
Thaller
M C
MC
Instituto di Microbiologia, Università, La Sapienza, Rome, Italy.
Berlutti
F
F
Dainelli
B
B
Pezzi
R
R
D001437:Bacteriuria / Q000382:microbiology; D003470:Culture Media; D004755:Enterobacteriaceae / Q000254:growth & development / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D014552:Urinary Tract Infections / Q000382:microbiology*
D003470:Culture Media