Interferon-alpha mimics effects of ovine trophoblast protein 1 on prostaglandin and protein secretion by ovine endometrial cells in vitro.
Ovine endometrial cells were isolated from ovariectomized oestrogen and progesterone-treated ewes and maintained in primary culture. In-vitro treatment with human interferon-alpha 2 (Roferon, Hoffman La Roche) (5, 50 IU/ml) or purified ovine trophoblast protein 1 (oTP-1, 30 ng/ml) significantly attenuated PGF2 alpha (25 +/- 17, 29 +/- 17, 28 +/- 9% +/- SEM of control [no in-vitro treatment = 100%] respectively, N = 4 ewes) and PGE (11 +/- 4, 16 +/- 4, 16 +/- 5% of control) release from the cultured cells. Fluorography of two dimensional polyacrylamide gel electrophoretic analyses of proteins secreted by the cells following 35S-methionine incorporation, revealed that synthesis and secretion of the same "pregnancy-related" proteins was stimulated by both interferon-alpha 2 and oTP-1. Thus, interferon-alpha 2 (which has sequence homology with oTP-1) acts on the ovine endometrium, eliciting similar biological responses to those of oTP-1.
Salamonsen
L A
LA
Medical Research Centre, Prince Henry's Hospital, Melbourne, Victoria, Australia.
Stuchbery
S J
SJ
O'Grady
C M
CM
Godkin
J D
JD
Findlay
J K
JK
D000818:Animals; D002478:Cells, Cultured; D004717:Endometrium / Q000187:drug effects*; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology*; D008186:Luteolytic Agents / Q000037:antagonists & inhibitors*; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012756:Sheep; D013997:Time Factors
D007370:Interferon Type I; D008186:Luteolytic Agents; D011257:Pregnancy Proteins; D011458:Prostaglandins E; D011506:Proteins; C020947:interferon tau