Characterization of doxylamine and pyrilamine metabolites via thermospray/mass spectrometry and tandem mass spectrometry.
Analysis of doxylamine N-oxide and pyrilamine N-oxide as synthetic standards and biologically derived metabolites by thermospray mass spectrometry (TSP/MS) provided [M + H]+ ions for each metabolite. TSP/tandem mass spectrometry (TSP/MS/MS) of the [M + H]+ ions provided fragment ions characteristic of these metabolites. In addition, TSP mass spectrometry and TSP/MS/MS analysis of ring-hydroxylated N-desmethyldoxylamine, N-desmethylpyrilamine and O-dealkylated pyrilamine is also reported. A fragmentation pathway for analysis by MS/MS of pyrilamine and its metabolites is also proposed. The results demonstrate the utility of TSP/MS for biologically derived metabolites of pyrilamine and doxylamine.
Korfmacher
W A
WA
National Center for Toxicological Research, Jefferson, Arkansas 72079.
Holder
C L
CL
Betowski
L D
LD
Mitchum
R K
RK
D000631:Aminopyridines / Q000032:analysis*; D000818:Animals; D001711:Biotransformation; D004319:Doxylamine / Q000032:analysis* / Q000378:metabolism; D005260:Female; D008253:Macaca mulatta; D008297:Male; D013058:Mass Spectrometry; D011725:Pyridines / Q000032:analysis*; D011738:Pyrilamine / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D000631:Aminopyridines; D011725:Pyridines; D004319:Doxylamine; D011738:Pyrilamine