High-resolution 13C NMR study of pressure effects on the main phase transition in L-alpha-dipalmitoyl phosphatidylcholine vesicles.
The effects of pressure on the liquid-crystalline to gel transition in vesicles of L-alpha-dipalmitoyl phosphatidylcholine were investigated by high-resolution proton-decoupled natural-abundance 13C NMR spectroscopy. The linewidths of several 13C resonances, including the choline methyl groups, carbonyl carbons, and choline methylene groups and the palmitoyl methyl groups are reported as a function of pressure at 52.7 degrees C. These preliminary NMR experiments clearly demonstrate that high-pressure, high-resolution proton-decoupled natural-abundance 13C NMR spectra are a promising tool to study the phase-transition behavior and the dynamics of model membrane systems.
Jonas
J
J
Department of Chemistry, School of Chemical Sciences, University of Illinois, Urbana 61801.
Xie
C L
CL
Jonas
A
A
Grandinetti
P J
PJ
Campbell
D
D
Driscoll
D
D
D015060:1,2-Dipalmitoylphosphatidylcholine; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008954:Models, Biological; D008968:Molecular Conformation; D011312:Pressure
D002247:Carbon Isotopes; D015060:1,2-Dipalmitoylphosphatidylcholine