The incorporation of monomethylethanolamine and dimethylethanolamine in fetal brain aggregating cell culture.
Fetal rat brain aggregating cell cultures were exposed to varying concentrations of [3H]monomethylethanolamine (MME) and [3H] dimethylethanolamine (DME). The rate of labeling of water-soluble compounds was more rapid and the amount of radioactivity present was greater than in the lipids. After a 72 hour incubation in the presence of millimolar concentrations of these nitrogenous bases, the major water-soluble products were the phosphorylated form of the bases. Little label was associated with the free bases or their cytidyl derivative. In the phospholipids, 97% of the radioactivity was recovered in phosphatidylmonomethylethanolamine (PMME) and 3% in phosphatidyldimethylethanolamine (PDME) or 95% in PDME and 5% in phosphatidylcholine (PC) after growth in presence of [3H]MME and [3H]DME respectively. The rate of formation of the radioactive products increased as function of the concentration of the nitrogenous base added up to 4 mM, the highest concentration employed. There was no significant difference in the pattern of labeling with cells grown in media devoid of methionine or choline. The turnover of the water-soluble metabolites was more rapid than in the phospholipids where an apparent half-life of 24 hours was calculated.
Dainous
F
F
Department of Biochemistry, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Kanfer
J N
JN
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D004983:Ethanolamines / Q000378:metabolism*; D005333:Fetus; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014316:Tritium
D003470:Culture Media; D004983:Ethanolamines; D010743:Phospholipids; D014316:Tritium; C017500:N-methylaminoethanol