Catalase-positive microperoxisomes in rat soleus and extensor digitorum longus muscle fiber types.
The size, distribution, and content of catalase-reactive microperoxisomes were studied cytochemically in slow-twitch oxidative (SO), fast-twitch oxidative glycolytic (FOG), and fast-twitch glycolytic (FG) fibers of soleus and extensor digitorum longus (EDL) rat muscles. Fiber types were classified on the basis of mitochondrial content and distribution, Z-band widths, and myofibril size and shape. Microperoxisomes were generally located between myofibrils at the I-bands. The absence of crystalloid inclusions prevented positive identification of microperoxisomes in nonreacted and aminotriazole-inhibited muscles. EDL and soleus SO fibers possessed the largest microperoxisomes, whereas FOG and FG fibers of the EDL contained small- to medium-sized microperoxisomes. Comparing either microperoxisome number per muscle fiber area or microperoxisome area per fiber area revealed significant differences between fiber types with this ranking: soleus SO greater than EDL SO greater than EDL FOG greater than EDL FG. The present observations demonstrate that the content of catalase-positive microperoxisomes is greatest in the oxidative muscle fiber types. These cytochemical findings account for the higher catalase activity in homogenates of soleus muscles as compared to that of EDL muscles, because the soleus contains more oxidative fibers than EDL.
Riley
D A
DA
Department of Anatomy & Cellular Biology, Medical College of Wisconsin, Milwaukee 53226.
Ellis
S
S
Bain
J L
JL
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002451:Cell Compartmentation; D006651:Histocytochemistry; D008297:Male; D008830:Microbodies / Q000201:enzymology*; D008854:Microscopy, Electron; D009132:Muscles / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats
D002374:Catalase