Targeting of mycotoxins to reticuloendothelial system of mice with carrier erythrocytes.
The tricothecene mycotoxin, T-2 toxin, was encapsulated in bovine erythrocytes for in vivo delivery of T-2 toxin to macrophages. Intraperitoneal injection of bovine carrier erythrocytes (5 X 10(8) cells) containing T-2 toxin saturated mouse liver uptake of erythrocytes by 6 h postinjection. At 24 h postinjection, 20% of the injected carrier cells containing toxin were localized in the liver of mice. Saturation of the liver uptake of bovine carrier cells was independent of encapsulated or free T-2 toxin. A dose of T-2 toxin sufficient to inhibit 50% of the macrophage protein synthesis was targeted to the liver via the carrier erythrocytes. A methodology for delivery of highly toxic molecules to liver macrophages is described.
DeLoach
J R
JR
Veterinary Toxicology and Entomology Research Laboratory, U.S. Department of Agriculture, College Station, Texas 77841.
Andrews
K
K
Naqi
A
A
D000818:Animals; D002250:Carbon Radioisotopes; D004912:Erythrocytes / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D012157:Mononuclear Phagocyte System / Q000378:metabolism*; D012717:Sesquiterpenes / Q000493:pharmacokinetics*; D013154:Spleen / Q000378:metabolism; D013395:Sucrose / Q000493:pharmacokinetics; D013605:T-2 Toxin / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014316:Tritium
D002250:Carbon Radioisotopes; D012717:Sesquiterpenes; D014316:Tritium; D013395:Sucrose; D013605:T-2 Toxin