Hexamminecobaltic chloride provides a simple method for marking neurones for electron microscopy.
Intracellular injection of hexamminecobaltic chloride into a neurone causes characteristic changes to the structure of its mitochondria. This provides a simple technique to label neuronal profiles for examination of their ultrastructure. Synaptic structures are not affected by hexamminecobaltic ions.
Littlewood
P M
PM
Department of Biology, University of Newcastle upon Tyne, U.K.
Simmons
P J
PJ
D000818:Animals; D002712:Chlorides; D003035:Cobalt; D006110:Grasshoppers; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure*; D011073:Polyamines; D013569:Synapses / Q000648:ultrastructure
D002712:Chlorides; D007202:Indicators and Reagents; D011073:Polyamines; C007936:hexaminecobalt (III) trichloride; D003035:Cobalt