Comparative studies between counterimmunoelectrophoresis and microprecipitation method of identification of human minute bloodstains.
A minute bloodstain on a thread 2 mm in length was tested to identify human origin by counterimmunoelectrophoresis (CIE), modified CIE, and microprecipitation method (MPM), using anti-human HbA serum. The detection limits expressed as the highest dilution of human blood on the thread in positive reaction were 1:160, 1:320, and 1:320 in CIE, modified CIE, and MPM, respectively. About 1 h was required to obtain the results. In CIE and modified CIE, the detection limits diminished according to the reduction of the samples from one half to one-eighth of the thread, but not in MPM.
Tajima
M
M
Department of Legal Medicine, School of Medicine, Iwate Medical University, Morioka, Japan.
Tokiwa
K
K
Katsura
S
S
D000328:Adult; D001801:Blood Stains; D011232:Chemical Precipitation; D003378:Counterimmunoelectrophoresis; D006441:Hemoglobin A / Q000032:analysis; D006801:Humans; D007122:Immunoelectrophoresis
D006441:Hemoglobin A