title: A peptide from the eel pancreas with structural similarity to human pancreatic secretory trypsin inhibitor.
abstract: The primary structure of a 61-amino-acid residue peptide from the pancreas of the European eel (Anguilla anguilla) has been established as E E K S G(5)L Y R K P(10)S C G E M(15)S A M H A(20)C P M N F(25)A P V C G(30)T D G N T(35)Y P N E C(40)S L C F Q(45)R Q N T K(50)T D I L I(55)T K D D R(60)C. There was no indication of microheterogeneity. This peptide shows structural similarity to pancreatic secretory trypsin inhibitors from several mammalian species and to a cholecystokinin-releasing peptide isolated from rat pancreatic juice. A comparison of the amino acid sequences of the peptides has identified a domain in the central region of the molecules that has been strongly conserved during evolution. In contrast, the amino acid sequence in the region corresponding to the reactive centre of the mammalian trypsin inhibitors is very poorly conserved in the eel peptide. The P1-P1' reactive site lysine-isoleucine (or arginine-isoleucine) bond in the mammalian trypsin inhibitors is replaced by a methionine-asparagine bond. This region does, however, show limited homology to the reactive centre of human alpha 1-protease inhibitor suggesting that the eel peptide may function as an inhibitor of other proteolytic enzymes in the pancreas.
authors: Clinical Research Group for Gastrointestinal Endocrinology of Max-Planck-Society, University of Göttingen, Federal Republic of Germany.
authors: J M
authors: JM
authors: Conlon
authors: L
authors: L
authors: Thim
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003545:Cysteine / Q000032:analysis; D004524:Eels; D006801:Humans; D008969:Molecular Sequence Data; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000032:analysis* / Q000302:isolation & purification; D014361:Trypsin Inhibitors / Q000032:analysis*; D014364:Tryptophan / Q000032:analysis
chemical_list: D000596:Amino Acids; D014361:Trypsin Inhibitors; D014359:Trypsin Inhibitor, Kazal Pancreatic; D014364:Tryptophan; D003545:Cysteine