Reversible effects of glycerol on the metabolism of platelets kept at room temperature.
The effects of the addition and removal of glycerol on the metabolic activities of human platelets were studied. Platelet concentrates (PC) with 20 ml plasma were stored with 3-7% (v/w) glycerol in 150-ml polyvinylchloride plastic bags for 2 days at 22 degrees C with constant agitation. Incubation of glycerol with platelets produced a dose-dependent inhibition of oxygen consumption. The inhibitions of glucose utilization and lactate production had reached the plateau level at 3% glycerol. The rate of adenosine triphosphate (ATP) generation of control platelets was 9.8 nmol/min/10(9) platelets, in which over 90% ATP generation was derived from oxidative phosphorylation. There was a dose-dependent decrease (up to 20%) by glycerol in the rate of platelet ATP generation. Glycerol inhibited glycolysis more than oxidative phosphorylation. However, the inhibition potency diminished with increasing concentrations of glycerol. The energy metabolism of platelets after removal of 5% glycerol was examined. Deglycerolized platelets after 1 hr incubation facilitated energy metabolism more strongly than that of 24 hr incubation. The platelet aggregation response to collagen was not impaired by a cycle of the addition and removal of glycerol. The results indicate that glycerol lowered the rate of ATP generation of platelets stored at 22 degrees C. However, the removal of glycerol reversed the decreased energy metabolism.
Shimizu
T
T
Aichi Blood Center, Japanese Red Cross, Seto.
Kouketsu
K
K
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D005990:Glycerol / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010101:Oxygen Consumption; D010976:Platelet Count; D013696:Temperature
D005990:Glycerol