Use of the frequencies of micronuclei as quantitative indicators of X-ray-induced chromosomal aberrations in human peripheral blood lymphocytes: comparison of two methods.
The yield of radiation-induced micronuclei in human lymphocytes was assessed by two methods, i.e., by incorporating bromodeoxyuridine or by inhibiting cytokinesis by cytochalasin for identification of cells which have undergone one cell division. The cytochalasin block method was found to be more efficient with a capacity to detect between 60 and 90% of the induced fragments. Dose-response characteristics and the results of fractionation experiments indicate that the yield of micronuclei reflects both classes of acentric fragments, i.e., those associated and independent of exchange type of aberrations.
Ramalho
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Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
Sunjevaric
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Natarajan
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AT
D001973:Bromodeoxyuridine / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000528:radiation effects*; D002869:Chromosome Aberrations; D003571:Cytochalasin B / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D009152:Mutagenicity Tests / Q000379:methods*
D003571:Cytochalasin B; D001973:Bromodeoxyuridine