title: The neurohormonal thymic microenvironment: immunocytochemical evidence that thymic nurse cells are neuroendocrine cells.
abstract: Thymic neuroendocrine cells were identified by immunofluorescence in the murine thymus through the use of monoclonal antibody A2B5, and specific polyclonal antisera against neurophysin (NP), oxytocin (OT) and arginine vasopressin (AVP). Two reactive regions were clearly identified: the subcapsular cortex and the medulla. A close correspondence was observed between A2B5-reactive and NP-immunoreactive cells in the medulla. An important epithelial population of the subcapsular cortex, the thymic nurse cells (TNCs), were found to be A2B5-positive and to contain immunoreactive NP, OT and AVP. The neuroendocrine nature of TNCs was further substantiated by their high reactivity with an antiserum against neuron-specific enolase. These observations demonstrate the presence in the thymus gland of an original neuroendocrine microenvironment which could be of functional importance in the mediation of central influences upon T lymphocyte differentiation.
authors: Laboratory of Radioimmunology, University of Liège-Sart Tilman, Belgium.
authors: V
authors: V
authors: Geenen
authors: M P
authors: MP
authors: Defresne
authors: F
authors: F
authors: Robert
authors: J J
authors: JJ
authors: Legros
authors: P
authors: P
authors: Franchimont
authors: J
authors: J
authors: Boniver
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001127:Arginine Vasopressin / Q000032:analysis*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009481:Neurophysins / Q000032:analysis*; D009490:Neurosecretory Systems / Q000032:analysis / Q000166:cytology; D010121:Oxytocin / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis* / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D009481:Neurophysins; D001127:Arginine Vasopressin; D010121:Oxytocin