title: Increase in mitochondrial GOT (m-GOT) activity after chronic alcohol consumption: clinical and experimental observations.
abstract: In order to clarify the origin and the mechanism of increased serum activity of glutamic oxalacetic transaminase (GOT) in chronic alcoholics, clinical and experimental investigations were carried out. Mitochondrial (m-GOT) and cytosolic GOT (c-GOT) isoenzymes were separated chromatographically by using a mini-column packed with Sephadex A50. Sixty percent of 63 alcoholics had elevated serum GOT. The m-GOT activity in alcoholics with total serum GOT activity of over 50 Karmen Units was 17.2 +/- 1.6 K.U. and the m-GOT/GOT ratio was the highest when compared to those in non-alcoholic liver diseases. In in vitro study, six hours of incubation of isolated hepatocytes from rats fed ethanol chronically resulted in an increased leakage of m-GOT into the incubation medium and also showed a tendency of a higher m-GOT/GOT ratio than that from control rats. The m-GOT activity thus released into the medium showed a highly significant inverse correlation with the viability of hepatocytes. These data suggest that m-GOT substantially contributes to an increased serum GOT often observed in chronic alcoholics.
authors: Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors: F
authors: F
authors: Okuno
authors: H
authors: H
authors: Ishii
authors: K
authors: K
authors: Kashiwazaki
authors: S
authors: S
authors: Takagi
authors: Y
authors: Y
authors: Shigeta
authors: M
authors: M
authors: Arai
authors: T
authors: T
authors: Takagi
authors: Y
authors: Y
authors: Ebihara
authors: M
authors: M
authors: Tsuchiya
mesh_terms: D000437:Alcoholism / Q000201:enzymology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood*; D003600:Cytosol / Q000201:enzymology; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008099:Liver / Q000201:enzymology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007527:Isoenzymes; D001219:Aspartate Aminotransferases