Determination of oxidizable inorganic anions by high-performance liquid chromatography with fluorescence detection and application to the determination of salivary nitrite and thiocyanate and serum thiocyanate.
A sensitive method for the simultaneous determination of oxidizable inorganic anions (sulphide, thiocyanate, thiosulphate and nitrite) was developed by use of high-performance liquid chromatography and fluorimetric detection based on the formation of fluorescent cerium(III) by a redox reaction with cerium(IV). The detection limits are 0.1 nmol for both thiocyanate and nitrite, 0.3 nmol for thiosulphate and 0.8 nmol for sulphide per 10-microliters injection volume. This system can be utilized for the determination of salivary thiocyanate and nitrite and serum thiocyanate.
Tanabe
S
S
Meiji College of Pharmacy, Tokyo, Japan.
Kitahara
M
M
Nawata
M
M
Kawanabe
K
K
D000838:Anions / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D009573:Nitrites / Q000032:analysis*; D012463:Saliva / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000032:analysis; D013696:Temperature; D013861:Thiocyanates / Q000032:analysis* / Q000097:blood
D000838:Anions; D007202:Indicators and Reagents; D009573:Nitrites; D013440:Sulfides; D013861:Thiocyanates