title: Determination of deoxyspergualin by gas chromatography-selected-ion monitoring.
abstract: A gas chromatographic-selected-ion monitoring method was developed for the sensitive and specific determination of deoxyspergualin in dog plasma using a capillary column and a C8-amide homologue of deoxyspergualin as an internal standard. Extraction and purification of deoxyspergualin from its constituents, 7-guanidinoheptanamide and glyoxylylspermidine, and its possible metabolites was achieved by using CM-Sephadex C-25 column chromatography with stepwise elution with sodium chloride solution. Treatment of deoxyspergualin with acetylacetone resulted in the formation of the volatile pyrimidine derivative of 7-guanidinoheptanamide accompanying hydrolytic cleavage in the alpha-hydroxyglycine moiety of deoxyspergualin. Deoxyspergualin could be determined in a concentration of 10 ng/ml in plasma. The method was applied to the determination of deoxyspergualin in plasma during and after intravenous infusion into dogs.
authors: Research Laboratory, Nippon Kayaku Co., Ltd., Tokyo, Japan.
authors: K
authors: K
authors: Yamashita
authors: K
authors: K
authors: Watanabe
authors: R
authors: R
authors: Koga
authors: S
authors: S
authors: Mizuguchi
authors: Y
authors: Y
authors: Hashimoto
authors: T
authors: T
authors: Nakamura
authors: H
authors: H
authors: Umezawa
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry; D006146:Guanidines / Q000097:blood / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D007262:Infusions, Intravenous
chemical_list: D006146:Guanidines; D007202:Indicators and Reagents; C037258:gusperimus