Determination of boldenone sulfoconjugate and related steroid sulfates in equine urine by high-performance liquid chromatography/tandem mass spectrometry.
Sulfoconjugated anabolic steroids were separated by micro-bore high-performance liquid chromatography. The eluent was introduced into the atmospheric pressure ion source of the triple-quadrupole mass spectrometer via an ion spray liquid chromatograph/mass spectrometer interface operated in the negative ion mode. The limit of detection was 10 pg on-column by selected ion monitoring of the molecular ion and the response increased linearly over a concentration range of 2.4 orders of magnitude. Following work-up by a liquid-solid extraction procedure of equine urine samples, full-scan daughter ion spectra of boldenone sulfate could be obtained up to 17 days after a therapeutic dose of boldenone undecylenate to a horse.
Weidolf
L O
LO
Equine Drug Testing and Toxicology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14850.
Lee
E D
ED
Henion
J D
JD
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006736:Horses; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D013739:Testosterone / Q000031:analogs & derivatives* / Q000652:urine
D013739:Testosterone; C013915:boldenone