[Isolation and detection of antibodies against nucleotide sequences. A study with oligonucleotide pApCpApC].
New methods for preparation and detection of antibodies to nucleotide sequences were developed. An efficient method for conjugation of oligonucleotides to proteins based on the activation of 5'-terminal oligonucleotide phosphate with dimethylaminopyridine was employed. Oligonucleotide pApCpApC was conjugated to bovine serum albumin with a 90% yield. Rabbit antibodies to the oligonucleotide were raised and characterized in terms of their specificity to nucleotide sequences. Affinity labeling with alkylating oligonucleotide derivatives was proposed as a method for detecting low affinity antibodies to nucleic acids.
Brosalina
E B
EB
Vlasov
V V
VV
Ivanova
E M
EM
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D009838:Oligodeoxyribonucleotides
Poluchenie i opredelenie antitel k nukleotidnym posledovatel'nostiam. Issledovanie na primere oligonukleotida pApCpApC.