A novel gastrin-processing pathway in mammalian antrum.
An antiserum, L221, has been developed that is specific for the C-terminal region of the N-terminal tridecapeptide (i.e., 1-13) fragment of the acid-stimulating hormone, G17. In contrast to N-terminal G17 antisera previously used to estimate 1-13 G17, L221 does not cross-react with other N-terminal gastrin fragments or with C-terminal extensions of G17. Using L221 in conjunction with conventional gastrin antisera, and reversed-phase HPLC, it has been possible to identify in addition to 1-13 G17 a further, formerly unrecognised gastrin fragment, 1-11 G17, in stomach extracts. The production of 1-13 G17, 1-11 G17 and other gastrin forms such as the biologically active hexapeptide G6 which is known to occur naturally cannot be explained by tryptic cleavage of progastrin. Instead, their biosynthesis could be explained by the actions of an enzyme with an endopeptidase 24.11-like specificity. In porcine antrum, unsulphated and sulphated G17 are present in similar amounts, but unsulphated 1-13 G17 was about twice as abundant as sulphate 1-13 G17. This is consistent with previous in vitro findings that endopeptidase 24.11 has a higher affinity for the Ala-11-Tyr-12 and Gly-13-Trp-14 bonds in unsulphated G17, than in sulphated G17. The results suggest a novel albeit minor, processing pathway for gastrin biosynthesis in pig antrum involving an enzyme resembling endopeptidase 24.11.
Power
D M
DM
MRC Secretory Control Research Group, Physiological Laboratory, University of Liverpool, U.K.
Dimaline
R
R
Balaspiri
L
L
Dockray
G J
GJ
D000595:Amino Acid Sequence; D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D011706:Pyloric Antrum / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D013045:Species Specificity; D013552:Swine
D005755:Gastrins