title: Solid-phase radioimmunoassay of human complement fragment C5a.
abstract: In this competitive RIA for determining concentrations of human C5a in biological fluids and in buffers, labeled C5a and sample are allowed to compete for binding to a limited amount of goat antibody to human C5a in solution. Free and bound tracer are then separated by a second antibody (rabbit anti-goat IgG) immobilized on paramagnetic particles. Total incubation time for this assay is 70 min. Sensitivity, precision, and analytical recovery of this assay compare well with those of a reference method.
authors: Ciba-Corning Diagnostics, E. Walpole, MA 02032.
authors: S
authors: S
authors: Chang
authors: A
authors: A
authors: Leo-Mensah
authors: J
authors: J
authors: Campbell
authors: M
authors: M
authors: Stastny
authors: R A
authors: RA
authors: Patrick
mesh_terms: D001667:Binding, Competitive; D003182:Complement C5 / Q000032:analysis*; D015936:Complement C5a; D004355:Drug Stability; D006358:Hot Temperature; D006801:Humans; D011863:Radioimmunoassay / Q000592:standards; D011933:Reagent Kits, Diagnostic
chemical_list: D003182:Complement C5; D011933:Reagent Kits, Diagnostic; D015936:Complement C5a