Type IV collagen-degrading enzyme activity in human serum.
Type IV collagen-degrading enzyme activity was detected in human serum. Serum was preincubated with 4-aminophenylmercuric acetate and trypsin to activate the enzyme prior to assay. Type IV collagen, purified from human placentas and radiolabeled with [1-14C] acetic anhydride, was used as the substrate. The enzyme activity was measured at pH 7.5 and inhibited by treatment with ethylenediaminetetraacetic acid or heat. The assay of type IV collagen-degrading enzyme in human serum might be useful for estimating the degradation of type IV collagen.
Hashimoto
N
N
First Department of Internal Medicine, Okayama University Medical School, Japan.
Kobayashi
M
M
Watanabe
A
A
Higashi
T
T
Tsuji
T
T
D001798:Blood Proteins / Q000032:analysis; D003094:Collagen / Q000378:metabolism*; D006801:Humans; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D001798:Blood Proteins; C010024:4-aminophenylmercuriacetate; D003094:Collagen; D014357:Trypsin; D010662:Phenylmercuric Acetate