title: Phosphorus nuclear magnetic resonance in isolated perfused rat pancreas.
abstract: Phosphorus nuclear magnetic resonance spectroscopy was applied to measure phosphorus energy metabolites in isolated perfused rat pancreas. The gland was perfused with a modified Krebs-Henseleit solution at room temperature (25 degrees C). 31P resonances of creatine phosphate (PCr), ATP, ADP, inorganic phosphate (Pi), and phosphomonoesters (PMEs) were observed in all the preparations of pancreas. In different individual preparations, the resonance of PCr varied, but those of ATP were almost the same. The initial levels of PCr and ATP in individual preparations, however, remained almost unchanged during perfusion with the standard solution for 2 h. When the perfusion was stopped, the levels of ATP and PCr decreased, while the levels of PME and Pi increased. At that time, the Pi resonance shifted to a higher magnetic field, indicating that the tissue pH decreased. On reperfusion, the tissue levels of phosphorus compounds and the tissue pH were restored to their initial resting levels. Continuous infusion of 0.1 microM acetylcholine caused marked and sustained increases in the flow of pancreatic juice and protein output. During the stimulation the tissue levels of phosphorus compounds remained unchanged, while the tissue pH was decreased slightly (0.05 pH unit).
authors: Department of Physiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors: T
authors: T
authors: Matsumoto
authors: T
authors: T
authors: Kanno
authors: Y
authors: Y
authors: Seo
authors: M
authors: M
authors: Murakami
authors: H
authors: H
authors: Watari
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D009943:Organophosphorus Compounds / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism*; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000227:Adenine Nucleotides; D009943:Organophosphorus Compounds; D010710:Phosphates; D010758:Phosphorus