The osmotic permeability of isolated calf pulmonary artery endothelial cells.
Osmotic permeability coefficients, PF, for water in isolated calf pulmonary artery endothelial cells determined over the temperature range 41 to 20 degrees C are 311.10(-5) cm.s-1 at 37 degrees C and 159.10(-5) cm.s-1 at 20 degrees C. The value at 37 degrees C is close to that reported earlier for the diffusional permeability coefficient, PD. The PF/PD ratio is 1.0 at 37 degrees C. The PF values are within the range of values extrapolated for filtration permeability in pulmonary endothelium. The temperature dependence expressed as the activation energy is 7.2 kcal.mol-1. The product of hydraulic conductivity, Lp (or PF) and of viscosity changes in water is not constant from 37 to 20 degrees C. These results can be interpreted to indicate a similar pathway for water whether under diffusional or osmotic gradients.
Garrick
R A
RA
Division of Science and Mathematics, Fordham University, College at Lincoln Center, New York, NY.
DiRisio
D J
DJ
Giannuzzi
R
R
Cua
W O
WO
Ryan
U S
US
Chinard
F P
FP
D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D009994:Osmolar Concentration; D009995:Osmosis; D011651:Pulmonary Artery / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics; D014783:Viscosity; D014867:Water / Q000378:metabolism
D014867:Water