Is the acetylcholine releasing protein mediatophore present in rat brain?
Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatophore activity with properties close to those described for the Torpedo protein (extractability, sensitivity to calcium, and effect of the drug cetiedil). The activity was more abundant in synaptosomal membranes than in mitochondrial or myelinic membranes and in cholinergic areas as compared to cerebellum.
Israël
M
M
Département de Neurochimie, CNRS, Gif-sur-Yvette, France.
Lesbats
B
B
Morel
N
N
Manaranche
R
R
Le Gal la Salle
G
G
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D008081:Liposomes; D008297:Male; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011510:Proteolipids; D051381:Rats; D011919:Rats, Inbred Strains; D013570:Synaptic Membranes / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure
D008081:Liposomes; D009419:Nerve Tissue Proteins; D011510:Proteolipids; C050299:mediatophore; C020485:proteoliposomes; D000109:Acetylcholine