Effects of antibodies against odorant binding proteins on electrophysiological responses to odorants.
Monoclonal antibodies against two olfactory mucosal proteins, one with affinity for anisole-like and the other for benzaldehyde-like compounds, were applied to mouse olfactory epithelium. Responses to three odorants (anisole, benzaldehyde and amyl acetate) were measured. Of 26 antibodies, three (12%) inhibited responses only to the odorant with affinity for the antigen, nine (35%) inhibited responses to all three odorants, and 14 (54%) were without effect. None reduced responses by as much as 50%. The data support the hypothesis that there is a class of related proteins in olfactory neuronal cell membranes that function as receptor molecules and that other mechanisms also mediate odorant stimulation.
Price
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S
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Willey
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A
D000818:Animals; D000840:Anisoles / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D000906:Antibodies / Q000502:physiology; D001547:Benzaldehydes / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D002352:Carrier Proteins / Q000276:immunology / Q000502:physiology*; D004285:Dogs; D004594:Electrophysiology; D008297:Male; D051379:Mice; D009812:Odorants; D009831:Olfactory Mucosa / Q000032:analysis / Q000187:drug effects / Q000502:physiology*; D000439:Pentanols / Q000494:pharmacology; D011984:Sensory Receptor Cells
D000840:Anisoles; D000906:Antibodies; D001547:Benzaldehydes; D002352:Carrier Proteins; D000439:Pentanols; C005716:amyl acetate; C032175:benzaldehyde