Increased ocular blood flow and 125I-albumin permeation in galactose-fed rats: inhibition by sorbinil.
125I-Albumin permeation and blood flow (assessed with 15 micron, 85Sr-labelled microspheres) were determined in the retina, choroid, anterior uvea, and brain of male Sprague-Dawley rats fed diets containing 50% dextrin (control) or 50% galactose. Blood flow was increased in the retina, choroid, and anterior uvea but not in the brain of rats fed galactose for 3 weeks and 3 months versus controls, and was normalized by sorbinil (an inhibitor of aldose reductase) in the 3-week group. After 8 months of galactose feeding, blood flow was reduced to normal levels in the retina and was slightly below normal in the choroid; blood flow remained elevated in the anterior uvea but was significantly lower than that observed at 3 weeks and at 3 months. In rats fed galactose for 8 months, sorbinil completely normalized blood flow in the choroid, and decreased, but did not normalize, blood flow in the anterior uvea. 125I-Albumin permeation was increased in the retina, choroid, and anterior uvea of rats fed 50% galactose for 3 weeks, 3 months, and 8 months versus controls, but was unchanged in the brain. Sorbinil normalized 125I-albumin permeation in all three ocular tissues in 8-month galactose-fed rats. Polyol levels were increased significantly in all three ocular tissues of 3-week galactose-fed rats; sorbinil markedly decreased, but did not normalize, polyol levels in all three tissues.(ABSTRACT TRUNCATED AT 250 WORDS)
Tilton
R G
RG
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110.
Chang
K
K
Weigel
C
C
Eades
D
D
Sherman
W R
WR
Kilo
C
C
Williamson
J R
JR
D000818:Animals; D001921:Brain / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects; D004032:Diet; D005123:Eye / Q000098:blood supply* / Q000378:metabolism; D005690:Galactose / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D048289:Imidazolidines; D007457:Iodine Radioisotopes; D010539:Permeability; D011108:Polymers / Q000378:metabolism; D012039:Regional Blood Flow / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000037:antagonists & inhibitors / Q000378:metabolism*
D007093:Imidazoles; D048289:Imidazolidines; D007457:Iodine Radioisotopes; D011108:Polymers; C024617:polyol; D012710:Serum Albumin, Bovine; C026411:sorbinil; D005690:Galactose