Utilization of mevalonate by aorta for the synthesis of medium-chain n-fatty acids (C8, C10) and acylglycerols.
[2-14C]Mevalonate was incorporated into acylglycerols by bovine aorta. The majority of the radioactivity was found in diacylglycerols. Hydrolysis, followed by analysis by thin-layer chromatography, paper chromatography, gas chromatography, and gas chromatography-mass spectrometry showed that the radioactivity was greatest in the medium-chain n-fatty acids, caprylic (C8) and capric (C10) acids. Triacylglycerols and diacylglycerols have important functions in the maintenance of the metabolic integrity of organs and their constituent cells. Therefore, medium-chain fatty acids may play significant roles, such as solubilization and transport of cholesterol and regulation of metabolism, as constituents of diacylglycerols. The results suggest that the trans-methylglutaconate shunt of mevalonate metabolism functions in the aorta.
Schwabauer
R A
RA
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Li
C H
CH
Adams
G S
GS
Gamble
W
W
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002417:Cattle; D005227:Fatty Acids / Q000096:biosynthesis*; D008401:Gas Chromatography-Mass Spectrometry; D005989:Glycerides / Q000096:biosynthesis*; D008798:Mevalonic Acid / Q000378:metabolism*
D005227:Fatty Acids; D005989:Glycerides; D008798:Mevalonic Acid