Autologous antibodies to human bladder cancer.
The autologous serologic reactivity of 13 patients with bladder cancer was evaluated using cell lines derived from each individual's own tumor as targets. Protein A and immune adherence assays were employed to determine antibody binding to the tumor targets at varying passage numbers. Autologous reactivity was found in 6 of the 13 cell lines tested. However, the titer was usually low regardless of the passage number. Seven autologous serum/cell line combinations were tested using both low and high passage cells as targets. In six of these combinations, the degree of antibody binding was similar with both low and high passage target cells. The incidence of autologous reactivity in the 12 patients with urothelial tumors was 50%.
Grossman
H B
HB
Department of Surgery, University of Michigan Medical Center, Ann Arbor 48109.
Wedemeyer
G
G
Stein
J
J
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000096:biosynthesis; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000276:immunology*; D002295:Carcinoma, Transitional Cell / Q000276:immunology; D002460:Cell Line; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D012397:Rosette Formation / Q000379:methods; D013205:Staphylococcal Protein A; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D013205:Staphylococcal Protein A