title: Induction of intercellular communications in epithelial cell cultures.
abstract: A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxically it is the standard fluorescent microscopy itself (that is, blue light of 320- to 480-nm wavelength) which induces rapid morphological alterations of cell culture followed by the transfer of fluorescent dye from one cell to another. Thus monitoring cell-cell dye coupling by fluorescent microscopy may itself induce the dye coupling in previously uncoupled epithelial cells.
authors: Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
authors: Sharovskaja YuYu
authors: L M
authors: LM
authors: Chailakhjan
authors: L B
authors: LB
authors: Margolis
mesh_terms: D000818:Animals; D002450:Cell Communication; D002460:Cell Line; D004848:Epithelium; D005456:Fluorescent Dyes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence
chemical_list: D005456:Fluorescent Dyes