Tissue specific opsonins for phagocytic cells and their different affinity for cholesterol-rich liposomes.
In accordance with the finding of our in vivo experiments reported earlier [(1983) Biochim. Biophys. Acta 761, 142-157; (1986) Biochim. Biophys. Acta 888, 184-190], the results of in vitro experiments show that Kupffer cells avidly take up cholesterol-poor but not cholesterol-rich liposomes, whereas splenic phagocytic cells take up preferentially cholesterol-rich rather than cholesterol-poor liposomes in the presence of serum. Evidence presented here suggests that serum contains opsonins specific for hepatic and splenic phagocytic cells and these opsonins have different affinities for cholesterol-rich and cholesterol-poor liposomes.
Moghimi
S M
SM
Department of Biochemistry, Charing Cross and Westminster Medical School, London, England.
Patel
H M
HM
D000327:Adsorption; D000818:Animals; D001769:Blood; D002784:Cholesterol / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004705:Endocytosis; D007728:Kupffer Cells / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D008099:Liver / Q000166:cytology; D009895:Opsonin Proteins / Q000502:physiology*; D010586:Phagocytes / Q000378:metabolism*; D051381:Rats; D013154:Spleen / Q000166:cytology
D008081:Liposomes; D009895:Opsonin Proteins; D002784:Cholesterol