title: Control of leucine transport in yeast by periplasmic binding proteins.
abstract: The concentrative inward transport of leucine in Saccharomyces carlsbergensis involves two transport systems (S1 and S2); S1 is a system of high affinity and low translocation velocity, and S2 is a system of low affinity and high translocation velocity. The inward transport process of the amino acid is discriminated into two kinetically defined steps: first, binding to periplasmic proteins and second, translocation across the plasmalemma. When cells were incubated with glucose to increase the metabolic energy charge, we observed that JTmax (maximum flux that each system can exhibit for the translocation step) increased for both systems. This increase in JTmax is due to variations in the parameters defining the initial step (Ks (apparent dissociation constant) and N (concentration of binding sites)): for S1, N1 increases and for S2, KS2 diminishes. Dissipation of the electrochemical proton gradient produced an increase of KS1 and a decrease of N2, resulting in a decrease of JTmax in both systems. Instead, osmotic shock decreases N1 and N2, which suggests that periplasmic components were removed, resulting also in a decrease of JTmax in both systems. These results are consistent with the proposition that the total unidirectional flux of the amino acid proceeds by means of a system of multiple components, with the simultaneous operation of two independent transport processes. We propose that the initial interaction of leucine with components of the cellular envelope might be the essential step for the subsequent translocation of the amino acid across the permeability barrier.
authors: Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
authors: S R
authors: SR
authors: Wainer
authors: A
authors: A
authors: Boveris
authors: E H
authors: EH
authors: Ramos
mesh_terms: D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism*; D004563:Electrochemistry; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D009997:Osmotic Pressure; D033902:Periplasmic Binding Proteins; D012440:Saccharomyces / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; C024913:LivK protein, E coli; D033902:Periplasmic Binding Proteins; D007930:Leucine