Rapid equilibrium radioimmunoassay for the amino-terminal propeptide of human type III procollagen.
This is an equilibrium-type radioimmunoassay for the amino-terminal propeptide of type III procollagen (PIIINP), which overcomes the problem of nonparallelism between the standard and human serum samples encountered with earlier assays. Proper selection of antiserum and reaction conditions diminishes interference from degradation products of the propeptide in serum. Because a rapid solid-phase-bound second-antibody step is included, the assay takes only 3 h. The intra-assay and the interassay CVs are both about 5%. In infants and children the concentration of PIIINP in serum closely parallels the growth-velocity curve. For 88 presumably healthy adults, the PIIINP concentration was 1.7-4.2 micrograms/L, about a third that measured with the previously available commercial assay. This is because of lack of inhibition by small Col 1 domain-related degradation products.
Risteli
J
J
Collagen Research Unit, Biocenter, Oulu, Finland.
Niemi
S
S
Trivedi
P
P
Mäentausta
O
O
Mowat
A P
AP
Risteli
L
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis*; D011347:Procollagen / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values
D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide