title: Low non-neuropathic tri-o-cresyl phosphate (TOCP) doses inhibit neuropathy target esterase near the neuropathic threshold in n-hexane pretreated hens.
abstract: Simultaneous intoxication with hexacarbon solvents and organophosphorus compounds has been considered a possible critical factor in some occupational neuropathies and their interactions proved to cause potentiation effects in hens [1-3]. A high degree of inhibition of neuropathy target esterase (NTE) is needed to develop organophosphorus induced polyneuropathy (OPIDP). In this work, the inhibition of NTE, BuChE and AChE by TOCP on control and n-hexane pretreated (7-15 days, 300 mg/kg per day) hens is studied. Using a single TOCP dose of 200 mg/kg, n-hexane pretreated hens showed synergistic effects, but no significant differences were observed in the inhibition of cholinesterases and NTE in brain or spinal cord. With lower TOCP dose (20 mg/kg) statistically significant differences were observed, which were not drastic but could be important because they involved an increase of inhibition up to critical threshold values (from 40-50% to 60-70% inhibition). However, no clinical effects were observed in these animals. Possible mechanisms of neurotoxic interaction are discussed.
authors: Departamento de Neuroquímica, Universidad de Alicante, Spain.
authors: M C
authors: MC
authors: Pellin
authors: E
authors: E
authors: Vilanova
authors: J
authors: J
authors: Barril
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002091:Butyrylcholinesterase / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002645:Chickens; D002802:Cholinesterases / Q000097:blood; D003408:Cresols / Q000633:toxicity*; D004347:Drug Interactions; D005260:Female; D006586:Hexanes / Q000633:toxicity*; D012584:Sciatic Nerve / Q000187:drug effects* / Q000201:enzymology; D013116:Spinal Cord / Q000187:drug effects* / Q000201:enzymology; D014317:Tritolyl Phosphates / Q000633:toxicity*
chemical_list: D003408:Cresols; D006586:Hexanes; D014317:Tritolyl Phosphates; C026385:n-hexane; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases; C025541:tri-o-cresyl phosphate