Monoclonal antibodies reacting with gliadin as tools for assessing antigenic structure responsible for exacerbation of celiac disease.
Monoclonal antibodies reactive with gliadin were prepared by fusion of spleen cells isolated from gliadin-immunized BALB/c mice with myeloma cells. The reactivity of mAbs with different preparations of gliadin and their enzymatic digest were measured using ELISA method. The mAb produced by GL 1 clone was shown to react preferentially with alpha-gliadin and its enzymatic digest.
Stoyanov
S
S
Institute of Molecular Genetics, Czechoslovak Academy of Science, Prague.
Tlaskalová-Hogenová
H
H
Kocna
P
P
Kristofová
H
H
Fric
P
P
Hekkens
W T
WT
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002446:Celiac Disease / Q000209:etiology*; D005903:Gliadin / Q000009:adverse effects / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D010940:Plant Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D010940:Plant Proteins; D005903:Gliadin