A cloned rat thymic epithelial cell line established from serum-free selective culture.
A serum-free system has been developed for selective growth and long-term culture of rat thymic epithelial cells. The growth media is a modification of McKeehan's WAJC 404, plus insulin, cholera toxin, dexamethasone, and epidermal growth factor. Cultures have been continuously passaged and maintained for over 6 mo., and a cloned cell line, TEA3A1, has been established. These cells are epithelial, judging by morphology and ultrastructure, and are positive for A2B5 and thymosin alpha 1 markers for thymic endocrine cells.
Piltch
A
A
W. Alton Jones Cell Science Center, Inc., Lake Placid, New York 12946.
Naylor
P
P
Hayashi
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D004847:Epithelial Cells; D007365:Intercellular Junctions / Q000648:ultrastructure; D051381:Rats; D013947:Thymosin / Q000096:biosynthesis; D013950:Thymus Gland / Q000166:cytology*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D013947:Thymosin