title: Correlation of DNA flow cytometric results and other prognostic factors in primary breast cancer.
abstract: The percentage of cells in S-phase and DNA-ploidy have been measured in 300 primary mammary carcinomas by means of DNA-flow cytometry (FCM). The data were compared with the age and menopausal status of the patients as well as with the size, regional lymph-node involvement, histologic type, grade and concentration of estrogen (ER) and progesterone (PR) receptors of the tumors. A DNA-diploid distribution of the G0/1-peak was found in 37.6% of the cases. The mean percentage of S-phase fractions was 4.83. DNA-aneuploid tumors had significantly higher amounts of S-phase fractions (6.12%) than DNA-diploid tumors (2.66%). There was also a significant correlation between the DNA measurement data (DNA-ploidy and S-phase fractions) and histologic grade, as well as the content of ER and PR, but not between DNA-ploidy, S-phase fractions, tumor size (T) and evidence of axillary lymph-node metastases. DNA-FCM gives a biological characterization of the tumor in addition to the histopathologic examination. The method can be used as a routine procedure because of the reliability and reproducibility of the results as well as the short time needed for the measurements.
authors: Institute of Comparative and Experimental Pathology, University of Heidelberg, FRG.
authors: G E
authors: GE
authors: Feichter
authors: A
authors: A
authors: Mueller
authors: M
authors: M
authors: Kaufmann
authors: D
authors: D
authors: Haag
authors: I A
authors: IA
authors: Born
authors: U
authors: U
authors: Abel
authors: K
authors: K
authors: Klinga
authors: F
authors: F
authors: Kubli
authors: K
authors: K
authors: Goerttler
mesh_terms: D000367:Age Factors; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008207:Lymphatic Metastasis; D008593:Menopause; D011379:Prognosis; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis
chemical_list: D004273:DNA, Neoplasm; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone