Loosening of the phage structure in a low ionic strength environment.
Structural parameters of phage T7 were compared in two frequently used Tris buffers of high and low ionic strength, in order to explain the different biological activity and drug-binding characteristics. Characteristics of the whole phage geometry were obtained by viscosimetry, static and quasi-elastic light-scattering and small-angle X-ray scattering. The latter method revealed dissimilarities in the intraphage DNA compactness, consistent with the findings of the optical absorption melting studies. Alterations in the particle dimensions determined in the same sample by different methods are discussed, and a model is constructed to explain the structural modifications that occur on lowering the ionic strength.
Rontó
G
G
Institute of Biophysics, Semmelweis Medical University, Budapest, Hungary.
Tóth
K
K
Csik
G
G
Feigin
L A
LA
Svergun
D T
DT
Dembo
A T
AT
Shtikova
E V
EV
D008027:Light; D009994:Osmolar Concentration; D012542:Scattering, Radiation; D012965:Sodium Chloride; D013604:T-Phages / Q000648:ultrastructure*; D014965:X-Rays
D012965:Sodium Chloride