Hydrogen-bonding effects and 13C-NMR of the DNA double helix.
13C-nmr chemical shifts of the nucleotides in DNA are sensitive to hydrogen bonding, especially for three of the carbons immediately bonded to exocyclic oxygen or nitrogen atoms acting as H-bond acceptors or donors. GuoC2, GuoC6 and ThdC4 are strongly deshielded (about 1 ppm) upon Watson-Crick pairing in oligodeoxynucleotide duplexes, regardless of the base sequence. Deshielding at these sites may be useful to distinguish bases involved in Watson-Crick pairs from unpaired bases.
Borer
P N
PN
NIH Research Resource, Syracuse University, NY 13244-1200.
LaPlante
S R
SR
Zanatta
N
N
Levy
G C
GC
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA; D006358:Hot Temperature; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy
D004247:DNA