title: Glucocorticoid binding sites in human temporal cortex.
abstract: This work describes the presence of glucocorticoid binding sites in human temporal cortex obtained following partial lobectomy in two epileptic patients. Using [3H]dexamethasone as radioligand and cold cortisol or RU 28362 as competitor we found an apparent Kd of approximately 2.8 nM with a Bmax of approximately 34 fmol/mg protein. The order of potency of various unlabeled steroids to compete for [3H]dexamethasone binding was as follows: RU 28362 = RU 38486 = cortisol = dexamethasone greater than progesterone greater than spironolactone greater than estradiol. These data provide evidence for an intracellular mechanism by which circulating glucocorticoids might regulate neuronal function in the human cortex.
authors: Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montreal, Canada.
authors: A
authors: A
authors: Sarrieau
authors: M
authors: M
authors: Dussaillant
authors: R M
authors: RM
authors: Sapolsky
authors: D H
authors: DH
authors: Aitken
authors: A
authors: A
authors: Olivier
authors: S
authors: S
authors: Lal
authors: W H
authors: WH
authors: Rostène
authors: R
authors: R
authors: Quirion
authors: M J
authors: MJ
authors: Meaney
mesh_terms: D000328:Adult; D000732:Androstanols / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D003907:Dexamethasone / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013702:Temporal Lobe / Q000378:metabolism*
chemical_list: D000732:Androstanols; D011965:Receptors, Glucocorticoid; C049154:11,17-dihydroxy-6-methyl-17-(1-propynyl)androsta-1,4,6-triene-3-one; D003907:Dexamethasone