Long-term treatment with cortisol: influence on proliferation, aging and glycosaminoglycan synthesis of cultured human diploid fibroblasts (WI-38).
Long-term treatment (several weeks and months) of cultured human diploid fibroblasts (WI-38) with cortisol (1.4 x 10(-7) M) stimulated proliferative activity and cellular glycosaminoglycan synthesis, thus counteracting the normal in vitro aging process. Characterization of the individual glycosaminoglycan types revealed an increased portion of cellular hyaluronic acid in cells treated with cortisol. Elevated synthesis of total glycosaminoglycans and, especially, of hyaluronic acid was found in the percellular pool (as determined by the amount liberated from the cells by trypsin treatment).
Sluke
G
G
Institut für Physiologische Chemie, Philipps-Universität, Lahnberge, Marburg, FRG.
Schachtschabel
D O
DO
D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004334:Drug Administration Schedule; D005347:Fibroblasts / Q000187:drug effects; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*
D006025:Glycosaminoglycans; D006854:Hydrocortisone