Schistosomin: a pronase-sensitive agent in the hemolymph of Trichobilharzia ocellata-infected Lymnaea stagnalis inhibits the activity of albumen glands in vitro.
The schistosome parasite, Trichobilharzia ocellata, nearly completely inhibits the reproductive activity of its intermediate host, Lymnaea stagnalis. The synthetic activity of albumen glands of infected snails at day 35 postinfection (p.i.) is only 1% of the control value. The parasite acts by humoral means. We tested the hypothesis that (a) specific humoral agent(s) is (are) involved and refer to this (these) agent(s) as schistosomin. The presence of schistosomin in the hemolymph of infected snails was investigated by using galactogen synthesis in albumen glands as an in vitro bioassay. The synthetic activity of albumen glands of noninfected snails decreased by about 50% during a 1-h incubation in the hemolymph of infected snails. This inhibition is attributed to schistosomin. Based on these results, with the present bioassay schistosomin appears in the hemolymph between days 28-36 p.i. onwards. Schistosomin is heat-stable (100 degrees C) and pronase-sensitive, and therefore it might have a peptide nature. Schistosomin suppresses the stimulating action of the female, gonadotrophic dorsal body hormone at relatively low doses, which suggests that it may compete with this hormone for the same receptors. The development of two other bioassays for schistosomin in our laboratory is discussed.
Joosse
J
J
Department of Biology, Vrije Universiteit, Amsterdam, The Netherlands.
van Elk
R
R
Mosselman
S
S
Wortelboer
H
H
van Diepen
J C
JC
D000818:Animals; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005088:Exocrine Glands / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000187:drug effects; D005685:Galactans / Q000096:biosynthesis; D006458:Hemolymph / Q000032:analysis*; D006790:Host-Parasite Interactions; D006863:Hydrogen-Ion Concentration; D007301:Insect Hormones / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D008195:Lymnaea / Q000235:genetics / Q000469:parasitology* / Q000502:physiology; D009929:Organ Size; D010455:Peptides / Q000097:blood* / Q000494:pharmacology; D011402:Pronase / Q000378:metabolism; D012551:Schistosomatidae / Q000378:metabolism*; D013696:Temperature
D005685:Galactans; D007301:Insect Hormones; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C054640:schistosomin; C007812:galactogen; D004247:DNA; D011402:Pronase