[The use of a radioimmunologic method of analysis of 11-deoxycortisol for the evaluation of the function of the pituitary-adrenal cortex system during the metopirone test].
A radioimmunoassay of II-deoxycortisol in human and animal blood plasma was developed. The antiserum used in the method was prepared against a conjugate of II-deoxycortisol-3(0-carboxymethyloxime) and bovine serum albumin. Specificity of the antiserum was determined in cross reactions using 32 steroids. High specificity of the antiserum allowed to determine II-deoxycortisol without preliminary chromatographic isolation. Sensitivity of the method enabled to detect 25 pg of II-deoxycortisol. The intra- and interassay variations were less than 10% and 15%, respectively. The method was used for evaluation of hypophysis-adrenal cortex system functions under the conditions of metopirone test.
Goncharov
N P
NP
Katsiia
G V
GV
Butnev
V Iu
VIu
Vorontsov
V I
VI
Khagundiukova
L B
LB
D015065:17-Hydroxycorticosteroids / Q000097:blood*; D000302:Adrenal Cortex / Q000502:physiology*; D000375:Aging / Q000502:physiology; D000818:Animals; D003350:Cortodoxone / Q000097:blood*; D005260:Female; D008297:Male; D008797:Metyrapone; D010215:Papio; D010903:Pituitary Gland, Anterior / Q000502:physiology*; D010913:Pituitary-Adrenal System / Q000502:physiology*; D011863:Radioimmunoassay
D015065:17-Hydroxycorticosteroids; D003350:Cortodoxone; D008797:Metyrapone
Primenenie radioimmunnogo metoda opredeleniia 11-dezoksikortizola dlia otsenki funktsii sistemy gipofiz-kora nadpochechnikov v usloviiakh metopironovogo testa.