Erythroid colony formation by human CFU-E is stimulated by compatible human serum but impaired by blood group antibodies.
Normal human serum was shown to stimulate the proliferation of human CFU-E when added into in vitro cultures of bone marrow cells. However, this was observed only when donors of serum and bone marrow cells were compatible on the basis of their blood groups. Absorption of incompatible sera removed the inhibition. Monoclonal anti-blood group antibodies exhibited the same pattern of CFU-E inhibition. The inhibition was complement-independent. Using suspension bone marrow cultures, it was shown that this blockage took place at an earlier differentiation step than the CFU-E level. This effect should be taken into account in studies of the inhibiting activities present in human anaemic sera.
Arnaud
S
S
Laboratoire de Biologie Cellulaire, Université Claude Bernard Lyon I, Villeurbanne, France.
Mouchiroud
G
G
Blanchet
J P
JP
D000017:ABO Blood-Group System / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000502:physiology; D001787:Blood Group Incompatibility / Q000097:blood*; D001790:Blood Physiological Phenomena; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000502:physiology*; D004920:Erythropoiesis; D006801:Humans; D007518:Isoantibodies / Q000502:physiology*
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007518:Isoantibodies