Immunoreactivity of aqueous extracts of rat and mouse tissue with anti-thymosin alpha 1, anti-bovine thymopoietin and anti-thymulin antibodies. Studies using immunoblotting.
Anti-thymosin alpha 1 monoclonal antibodies recognized, on immunoblots, 1 to 2 bands corresponding to molecules of 34 and 35 Kd when using aqueous extracts of thymus, spleen, kidney, liver, brain, pituitary and adrenal glands from rats or mice. Anti-bovine thymopoietin polyclonal antibodies, in the same conditions, labelled analogous 34, 35 and 35.5 Kd molecules in brain and thymus extracts but also a 40 Kd molecules in thymus and a 90 Kd in brain extracts. Anti-synthetic thymulin monoclonal antibodies recognized irregularly and poorly a 52 Kd molecule from thymus and brain extracts. These results suggest that thymopoietin, thymulin and specially Thymosin alpha 1 are first synthesized in large precursors. Finally, other organs seem capable of synthesizing thymosin alpha 1 and probably thymopoietin, but for thymulin, the results are too irregular to conclude.
Monier
J C
JC
Laboratoire d'Immunologie, U.F.R. de Médecine, Lyon, France.
Auger
C
C
Corvee
N
N
Stahli
C
C
Fabien
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D011919:Rats, Inbred Strains; D000077596:Thymalfasin; D013935:Thymic Factor, Circulating / Q000276:immunology*; D013946:Thymopoietins / Q000276:immunology*; D013947:Thymosin / Q000031:analogs & derivatives* / Q000276:immunology; D013950:Thymus Gland / Q000378:metabolism; D013951:Thymus Hormones / Q000276:immunology*; D014020:Tissue Extracts / Q000276:immunology*
D000911:Antibodies, Monoclonal; D013946:Thymopoietins; D013951:Thymus Hormones; D014020:Tissue Extracts; D013947:Thymosin; D003101:Collodion; D013935:Thymic Factor, Circulating; D000077596:Thymalfasin