Binding site of the rat liver specific monoclonal antibody.
A rat liver-specific antigen (RLSA) lost its binding ability to the corresponding monoclonal antibody after treatment with N-glycanase or sialidase, which suggested that the specific binding site might be in a portion of the sugar chain containing sialic acid. The specific antigen reacted with wheat germ agglutinin, lentil lectin, erythroagglutinating phytohemagglutinin and Ricinus communis agglutinin, but not with concanavalin A or peanut agglutinin. These results suggest that the specific antigen has asparagine-linked complex-type sugar chains which might be the binding sites of the monoclonal antibody.
Sato
K
K
Research Institute, Daiichi Seiyaku Co., Ltd., Tokyo, Japan.
Ikeda
T
T
Osada
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000276:immunology*; D008970:Molecular Weight; D051381:Rats
D000911:Antibodies, Monoclonal