A well-characterized plasma membrane fraction obtained from mouse peritoneal macrophages.
A new method was developed to isolate a plasma membrane fraction from lipopolysaccharide-stimulated mouse peritoneal macrophages. Colchicine treatment was followed by sucrose density-gradient centrifugation. Total yield of Na,K-ATPase, a marker of plasma membrane, was 60 +/- 1% with the specific activity of 37 +/- 3 mumol of Pi/mg of protein/h. The preparation contained 1 +/- 1% pinosomes, 2 +/- 1% lysosomes, 17 +/- 2% endoplasmic reticulum, 6 +/-1% mitochondria, and a negligible number of nuclei, as judged by distribution of markers.
Tomita
M
M
Department of Biochemistry, Saitama Medical School, Japan.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000648:ultrastructure*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000379:methods; D005260:Female; D008070:Lipopolysaccharides; D008264:Macrophages / Q000648:ultrastructure*; D051379:Mice; D008809:Mice, Inbred C3H; D013395:Sucrose
D008070:Lipopolysaccharides; D013395:Sucrose