Isolation of non-pigmented epithelial cells from rabbit ciliary body.
We describe a new technique for the separation and isolation of nonpigmented ciliary body epithelial cells from rabbit. Excised ciliary body is incubated in a medium buffered with EGTA to a free-Ca2+ concentration of 10(-8) M, and the nonpigmented cell layer is separated from the pigmented layer by microdissection under direct visualization. This technique yields intact cells which are greater than 99% nonpigmented. It can be used to produce preparations of nonpigmented cell plasma membrane and of viable whole cells, which may be useful for biochemistry, pharmacology, cell transport studies and tissue culture.
Fain
G L
GL
Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024-1771.
Cilluffo
M C
MC
Fain
M J
MJ
Lee
D A
DA
D000818:Animals; D002452:Cell Count; D002462:Cell Membrane; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002924:Ciliary Body / Q000166:cytology*; D003470:Culture Media; D046508:Culture Techniques; D004847:Epithelial Cells; D011817:Rabbits
D003470:Culture Media