title: DNA-nitrosourea interactions. High-performance liquid chromatography of cross-linked dinucleosides and substituted deoxynucleosides.
abstract: A reconstituted mixture of five cross-linked dinucleosides possibly involved in DNA-nitrosourea interactions, and of their degradation products (nucleobases, deoxynucleosides and mono- or disubstituted deoxynucleosides), was analysed by reversed-phase high-performance liquid chromatography using C18 columns and an diode-array detector. The chromatographic conditions for separating the twenty-one investigated compounds were optimized, and the compounds were identified by both their retention times and their UV spectra. A structure-retention time relationship was observed under suitable conditions and is discussed. Its validity was confirmed by the prediction of the retention time of a new cross-linked dinucleoside synthesized for this purpose.
authors: Laboratoire de Chimie Bio-Organique, Université des Sciences, Montpellier, France.
authors: A F
authors: AF
authors: Maggio
authors: A
authors: A
authors: Pompon
authors: M
authors: M
authors: Lucas
authors: J L
authors: JL
authors: Barascut
authors: J L
authors: JL
authors: Imbach
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009607:Nitrosourea Compounds / Q000032:analysis*; D009705:Nucleosides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009607:Nitrosourea Compounds; D009705:Nucleosides; D004247:DNA