title: In vivo red blood cell compatibility testing using indium-113m tropolone-labeled red blood cells.
abstract: In vivo radionuclide crossmatch is a method for identifying compatible blood for transfusion when allo- or autoantibodies preclude the use of conventional crossmatching techniques. A technique for labeling small volumes of donor red blood cells with [113mIn]tropolone is reported. The use of 113mIn minimizes the accumulation of background radioactivity and the radiation dose especially so when multiple crossmatches are performed. Labeling red cells with [113mIn]tropolone is faster and easier to perform than with other radionuclides. Consistently high labeling efficiencies are obtained and minimal 113mIn activity elutes from the labeled red blood cells. A case study involving 22 crossmatches is presented to demonstrate the technique. The radiation dose equivalent from 113mIn is significantly less than with other radionuclides that may be used to label red cells.
authors: Department of Nuclear Medicine, Victoria Hospital, London, Ont., Canada.
authors: G J
authors: GJ
authors: Morrissey
authors: D
authors: D
authors: Gravelle
authors: G
authors: G
authors: Dietz
authors: A A
authors: AA
authors: Driedger
authors: M
authors: M
authors: King
authors: T D
authors: TD
authors: Cradduck
mesh_terms: D000328:Adult; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D003508:Cycloheptanes; D004912:Erythrocytes; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D009942:Organometallic Compounds; D014334:Tropolone / Q000031:analogs & derivatives
chemical_list: D003508:Cycloheptanes; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C043895:indium tris(tropolonate); D014334:Tropolone