Regulation of expression of an auxin-induced soybean sequence by cadmium.
An auxin-regulated soybean sequence has been characterized and shown to be induced by the heavy metals cadmium, silver, and copper. Cadmium induces the accumulation of two size classes of mRNA: a 1-kilobase (kb) RNA class, which is the same size as the RNA class induced by auxin, silver, and copper, and a 1.4-kb RNA class. DNA sequence analysis of cDNA clones and a soybean genomic fragment has shown the presence of an intron in this gene. A restriction fragment probe isolated from the intron segment hybridizes specifically to the 1.4-kb mRNA. The transcription rate of this sequence is rapidly increased following exposure of soybean primary leaves to cadmium, as assayed by nuclear run-off transcription experiments. These results suggest that cadmium not only induces the transcription of a specific soybean sequence, but interferes with the processing of the precursor mRNA, resulting in the accumulation of the 1.4-kb mRNA precursor species.
Hagen
G
G
Department of Biochemistry, University of Missouri, Columbia 65211.
Uhrhammer
N
N
Guilfoyle
T J
TJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects*; D014158:Transcription, Genetic / Q000187:drug effects
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D002104:Cadmium; D004247:DNA