Continuous-flow fast atom bombardment-mass spectrometry of permethylated oligosaccharides: a comparative study of direct mixture analysis with packed capillary column liquid chromatography-fast atom bombardment-mass spectrometry.
Conventional positive fast atom bombardment (FAB) and continuous-flow FAB analysis were carried out with permethylated lacto-N-tetraose. This latter method, a new approach, has been used to analyze a mixture of permethylated oligosaccharides by liquid chromatography-mass spectrometry (LC-MS) with a packed capillary fused-silica column and the continuous-flow probe as interface. Under these conditions, we found the continuous-flow probe to be superior to the conventional probe because its low matrix level increased the signal-to-noise ratio. The analysis of the mixture of permethylated oligosaccharide alditols obtained from hen ovomucoid by LC-MS using the continuous-flow probe as interface is described.
Boulenguer
P
P
Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres Artois (UA du CNRS No. 217), Villeneuve d'Ascq, France.
Leroy
Y
Y
Alonso
J M
JM
Montreuil
J
J
Ricart
G
G
Colbert
C
C
Duquet
D
D
Dewaele
C
C
Fournet
B
B
D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis*; D010059:Ovomucin / Q000032:analysis; D013402:Sugar Alcohols / Q000032:analysis
D009844:Oligosaccharides; D013402:Sugar Alcohols; D010059:Ovomucin