Identification of proteins of the 40 S ribosomal subunit involved in interaction with initiation factor eIF-2 in the quaternary initiation complex by means of monospecific antibodies.
Monospecific polyclonal antibodies against seven proteins of the 40 S subunit of rat liver ribosomes were used to identify ribosomal proteins involved in interaction with initiation factor eIF-2 in the quaternary initiation complex [eIF-2 X GMPPCP X [3H]Met-tRNAf X 40 S ribosomal subunit]. Dimeric immune complexes of 40 S subunits mediated by antibodies against ribosomal proteins S3a, S13/16, S19 and S24 were found to be unable to bind the ternary initiation complex [eIF-2 X GMPPCP X [3H]Met-tRNAf]. In contrast, 40 S dimers mediated by antibodies against proteins S2, S3 and S17 were found to bind the ternary complex. Therefore, from the ribosomal proteins tested, only proteins S3a, S13/16, S19 and S24 are concluded to be involved in eIF-2 binding to the 40 S subunit.
Bommer
U A
UA
Academy of Sciences of the GDR, Department of Cell Physiology, Berlin-Buch.
Stahl
J
J
Henske
A
A
Lutsch
G
G
Bielka
H
H
D000818:Animals; D002499:Centrifugation, Density Gradient; D003432:Cross-Linking Reagents; D015852:Eukaryotic Initiation Factor-2; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D007118:Immunoassay; D008099:Liver / Q000648:ultrastructure*; D046911:Macromolecular Substances; D010448:Peptide Initiation Factors / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012358:RNA, Transfer, Met / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D003432:Cross-Linking Reagents; D015852:Eukaryotic Initiation Factor-2; D046911:Macromolecular Substances; D010448:Peptide Initiation Factors; D011506:Proteins; D012358:RNA, Transfer, Met; D012269:Ribosomal Proteins; C004805:5'-guanylylmethylenebisphosphonate; D006160:Guanosine Triphosphate