title: Effect of rhein on electrogenic chloride secretion in rabbit distal colon.
abstract: The effect of the laxative component rhein on electrical properties and Cl transport was investigated in partially stripped epithelial sheets of rabbit distal colon under short-circuit conditions. Whereas mucosal rhein had no effect, serosally applied rhein stimulated electrogenic Cl secretion in a dose-dependent manner with a half-maximal stimulation at 0.05 mmol/l. The stimulation was partially reversible by serosal bumetanide. Indomethacin preincubation inhibited the effect of rhein, but did not prevent theophylline-induced Cl secretion. The results suggest a prostaglandin-mediated effect of rhein on the apical Cl conductance of the colonic cells.
authors: Institut für Veterinärphysiologie, Freie Universität Berlin, BRD.
authors: W
authors: W
authors: Clauss
authors: G
authors: G
authors: Domokos
authors: E
authors: E
authors: Leng-Peschlow
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D002400:Cathartics / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D003106:Colon / Q000187:drug effects* / Q000502:physiology; D004553:Electric Conductivity; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D011453:Prostaglandins / Q000096:biosynthesis; D011817:Rabbits
chemical_list: D000880:Anthraquinones; D002400:Cathartics; D002712:Chlorides; D011453:Prostaglandins; D007213:Indomethacin; C020491:rhein