Androgen receptor-deficient Tfm cells in the mosaic epididymis of sex-reversed mice heterozygous for Tfm: an autoradiographic study with [3H]-dihydrotestosterone and [3H]-estradiol.
Testicular feminization (Tfm) in the mouse is characterized by androgen insensitivity of the target cells. We describe the presence of androgen-insensitive Tfm cells in the epididymis of mosaic mice produced by converting female carriers of the Tfm mutation (XTfm/X+) to males via the sex reversal factor (Sxr). The mosaic arises by random X-inactivation. In the epididymal duct, flat undifferentiated Tfm cells are interspersed between high columnar wild-type cells. By thaw-mount autoradiography we show that after injection of [3H]dihydrotestosterone, radioactivity is concentrated in the nuclei of high columnar wild-type cells, whereas the nuclei of the low cuboid Tfm cells remain free of nuclear radioactivity. After injection of [3H]estradiol, both Tfm and wild-type cells show nuclear labeling. Our observations demonstrate that Tfm cells in the mosaic epididymis selectively lack nuclear dihydrotestosterone-binding sites, whereas estradiol-binding sites are intact.
Drews
U
U
Anatomisches Institut, Universität Tübingen, Federal Republic of Germany.
Schleicher
G
G
Stumpf
W E
WE
Thiedemann
K U
KU
D013734:Androgen-Insensitivity Syndrome / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D013196:Dihydrotestosterone / Q000378:metabolism; D012734:Disorders of Sex Development; D004822:Epididymis / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006579:Heterozygote; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009030:Mosaicism; D011944:Receptors, Androgen / Q000378:metabolism*
D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D004958:Estradiol