Increased lethality and delay in the recovery of hemopoietic stem cells after irradiation in mice exposed to nitrous oxide.
Effects of N2O-exposure on the survival rate and growth kinetics of hemopoietic stem cells after irradiation were investigated. LD 50, 30 days after irradiation, was 300 rad for N2O exposed mice and over 550 rad for control mice. Recovery of the pluripotent hemopoietic stem cells (CFU-S) and granulocyte-macrophage progenitor cells (GM-CFC) was significantly delayed in the spleen of N2O-exposed mice after 150 rad irradiation, compared to that of control mice. However, in bone marrow, there was a significant but slight difference in the recovery of GM-CFC and no significant difference in the recovery of CFU-S between the two groups. These results suggest that N2O augments the damage of splenic hemopoiesis in irradiated mice, and this may be responsible for the increased hemopoietic death.
Konno
M
M
Department of Anesthesiology, School of Medicine, Iwate Medical University, Morioka, Japan.
Kirikae
T
T
Suzuki
K S
KS
Yoshida
M
M
Mori
K J
KJ
Wakusawa
R
R
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009609:Nitrous Oxide / Q000633:toxicity*
D003037:Cobalt Radioisotopes; D009609:Nitrous Oxide