title: The generation of human lymphokine-activated killer cells in various serum-free media.
abstract: Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).
authors: Cellular Immunology Section, Biotherapeutics Inc., Franklin, TN 37064.
authors: M R
authors: MR
authors: Jadus
authors: G B
authors: GB
authors: Thurman
authors: A
authors: A
authors: Mrowca-Bastin
authors: J R
authors: JR
authors: Yannelli
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007116:Immunization, Passive; D007167:Immunotherapy; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008222:Lymphokines / Q000494:pharmacology
chemical_list: D003470:Culture Media; D008222:Lymphokines