Immunocytochemistry of a vasopressin (AVP) receptor with anti-idiotype antibody: inhibition of staining with a peptide (PVA) encoded by an RNA that is complementary to AVP mRNA.
Immunocytochemical staining of putative presynaptic (auto-) receptors associated with vasopressin (AVP) neurons by anti-idiotype antibody can be markedly reduced or abolished by preincubation of the antibody with peptide PVA. This peptide, Ser-Ser-Trp-Ala-Val-Leu-Glu-Val-Ala, represents amino acids encoded by a nucleotide sequence complementary to the mRNA code of AVP. These results suggest that PVA may have some binding characteristics similar to the AVP autoreceptor.
Knigge
K M
KM
Neuroendocrine Unit, University of Rochester, School of Medicine and Dentistry, NY 14642.
Piekut
D T
DT
Berlove
D
D
D000818:Animals; D007130:Immunoglobulin Idiotypes; D007150:Immunohistochemistry; D008297:Male; D009842:Oligopeptides / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000276:immunology* / Q000378:metabolism; D017483:Receptors, Vasopressin
D007130:Immunoglobulin Idiotypes; D009842:Oligopeptides; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; C056203:peptide PVA