Vaccination of mice against Leishmania mexicana amazonensis with microsomal fraction associated with BCG.
Attempts to develop a satisfactory vaccine against New World cutaneous leishmaniasis have been made with varying success. We found that in mice, pretreated subcutaneously with 2 X 10(6) BCG organisms 2 weeks prior to immunization at the same site with as low as 10 micrograms of a microsomal preparation (Pol-F) of Leishmania mexicana amazonensis, a profound alteration was observed in the course of infection produced by inoculation of virulent amastigotes of the same strain. The BCG-Pol-F vaccine preparation was consistently shown to have a protective capacity associated with larger classical cellular immune response (skin test) and higher specific antibody titres in response to leishmanial challenge dose (1 X 10(6) amastigotes/mouse).
Goncalves da Costa
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Départemento de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Santos
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Lagrange
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D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed; D007116:Immunization, Passive; D007894:Leishmania mexicana / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008861:Microsomes / Q000276:immunology*; D012867:Skin / Q000473:pathology; D014611:Vaccination
D001500:BCG Vaccine