Production of antiserum against antitumor protein-bound polysaccharide preparation, PSK (Krestin) and its pharmacological application.
Antiserum against a protein-bound polysaccharide preparation (PSK) was produced by immunizing New Zealand White rabbits with PSK. The intestinal absorption of PSK in mice was visualized by indirect immunofluorescent staining with anti-PSK serum. The change in blood levels of 14C after oral administration of 14C-PSK and the recovery of 14C by antiserum were determined. The results indicated that antigenic epitopes in PSK are not completely destroyed during the process of digestion, absorption and distribution, but the changes of serum levels of 14C radioactivity differ from those of immunoreactive radioactivity. These results suggest that multiple processes are involved in the fate of PSK administered orally.
Endoh
H
H
Biomedical Research Laboratories, Kureha Chemical Industry Co. Ltd, Tokyo, Japan.
Matsunaga
K
K
Yoshikumi
C
C
Kawai
Y
Y
Suzuki
T
T
Nomoto
K
K
D000284:Administration, Oral; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000276:immunology*; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D007150:Immunohistochemistry; D007422:Intestines / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011509:Proteoglycans / Q000276:immunology* / Q000493:pharmacokinetics; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000903:Antibiotics, Antineoplastic; D007106:Immune Sera; D011509:Proteoglycans; C010770:polysaccharide-K