Determination of human fibroblasts metabolism in vitro by gas chromatography-mass spectrometry of cell-excreted metabolites.
In order to develop an approach to the study of cell metabolism in vitro, we undertook the determination of metabolites excreted by human skin diploid fibroblasts into culture medium using high-resolution gas chromatography in combination with mass spectrometry. Chromatographic and mass spectrometric characteristics of 29 metabolites have been obtained, and 11 of the metabolites have been identified. The excreted metabolites reflect the activity of certain metabolic processes in fibroblasts in vitro. A comparison of chromatographic and mass spectrometric characteristics of the cell metabolites and of those excreted from the body in urine showed most of the metabolites excreted by fibroblasts to be different from the urine metabolites. The possibility of secondary conversion of cell metabolites in the organism and the specificity of metabolism in cells of different tissues are discussed.
Antoshechkin
A G
AG
Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
Tatur VYu
Perevezentseva
O M
OM
Maximova
L A
LA
D000328:Adult; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003470:Culture Media / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D012867:Skin / Q000378:metabolism
D003470:Culture Media