Effect of free fatty acids on the bioavailability of plasma testosterone and dihydrotestosterone.
Free fatty acids (FFA) are known to interfere with the binding of thyroid hormone and estrogens to circulating proteins, but their effect on androgen binding is unknown. The effect of linoleic, oleic and palmitic acids at physiological concentrations on the binding of testosterone (T) and dihydrotestosterone (DHT) to circulating proteins was evaluated in vitro, using equilibrium dialysis and ammonium sulfate precipitation techniques. The results indicate that FFA can inhibit T binding to albumin and SHBG. They also can inhibit DHT binding to albumin, whereas DHT binding to SHBG is not altered, suggesting that FFA at physiological concentrations may be important regulators of bioavailability of T to tissues.
Mooradian
A D
AD
Department of Medicine, Veterans Administration Medical Center, Sepulveda, CA 91343.
Pamplona
D M
DM
Viosca
S P
SP
Korenman
S G
SG
D001682:Biological Availability; D001798:Blood Proteins / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D013739:Testosterone / Q000097:blood*
D001798:Blood Proteins; D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D013196:Dihydrotestosterone; D019301:Oleic Acid; D019308:Palmitic Acid; D013739:Testosterone; D019787:Linoleic Acid