Preliminary profiles of 13,14-dihydroprostaglandin F2 alpha synthetase catalyzing conversion of 13,14-dihydro-15-ketoprostaglandin F2 alpha to 13,14-dihydroprostaglandin F2 alpha in rat ovary.
In the rat ovary 13,14-dihydroprostaglandin F2 alpha (13,14H2-PGF2 alpha) synthetase required NADPH much more than NADH as a co-factor. 13,14H2-PGF2 alpha synthetase activity was NADPH concentration-dependent and was significantly inhibited by NADP, in the presence of NADPH, by indomethacin and by oxidized glutathione.
Inazu
N
N
Department of Pharmacology and Toxicology, Tokyo College of Pharmacy, Japan.
Harasawa
Y
Y
Satoh
T
T
D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives*; D005260:Female; D005978:Glutathione / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D011460:Prostaglandins F / Q000096:biosynthesis* / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011460:Prostaglandins F; C026527:13,14-dihydroprostaglandin F2alpha; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D009249:NADP; D015237:Dinoprost; D005978:Glutathione; D007213:Indomethacin