title: [Identification of pro-opiomelanocortin and its C-terminal fragments in the mammalian thymus].
abstract: Immunoreactive alpha-, beta-, gamma-endorphins and beta-lipotropin were detected in perfused calf thymus extracts at the following concentrations (fmol/mg) tissue, M +/- m): 1.32 +/- 0.08, 1.53 +/- 0.45, 0.0186 +/- 0.0022 and 0.741 +/- 0.157, respectively. It was demonstrated for all ligands tested that the synthetic peptide and increasing amounts of the extract cause a similar displacement of the corresponding 125I-peptide from its complex with specific antiserum. Using the immunoblotting technique with a highly specific antiserum to bovine beta-lipotropin, the extracts of calf thymus, rat thymocytes and bovine hypophysis were found to contain two polypeptides with Mr of 32 and 14 kD, whose mobility corresponds to that of proopiomelanocortin and beta-lipotropin.
authors: S F
authors: SF
authors: Pshenichkin
authors: A A
authors: AA
authors: Zozulia
authors: E A
authors: EA
authors: Kizim
authors: A D
authors: AD
authors: Dmitriev
mesh_terms: D000818:Animals; D002417:Cattle; D004723:Endorphins / Q000032:analysis; D008297:Male; D010446:Peptide Fragments / Q000032:analysis*; D011333:Pro-Opiomelanocortin / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000032:analysis*; D008083:beta-Lipotropin / Q000032:analysis
chemical_list: D004723:Endorphins; D010446:Peptide Fragments; D011333:Pro-Opiomelanocortin; D008083:beta-Lipotropin
vernacular_title: Identifikatsiia proopiomelanokortina i ego C-kontsevykh fragmentov v timuse mlekopitaiushchikh.