[Morphocytochemical reaction of the lymph nodes in dogs to the administration of lysozyme].
By means of morphological, morphometrical and histochemical methods pelvic and tracheobronchial lymph nodes have been studied in dogs and concentration of lysozyme has been estimated in blood serum, in lymph and the lymph nodes after a single intramuscular injection of lysozyme (2 mg/kg of body mass). In the material investigated total concentration of lysozyme reaches its maximal values in 6 h after injection, then it gradually decreases and in 48 h reaches its control level. Morphometrically changes in cell composition are revealed predominantly of immune-competent cells in T- and B-dependent zones of the lymph nodes. Thus, the volumetric part of lymphoblasts in the germinative centers of the lymphoid nodules reaches its maximal indices by 48 h after lysozyme injection, while plasmatization of the paracortical zone and of medullary cords increases up to the 7th day. By the 14th day the volumetric part of lymphoblasts, immunoblasts and plasmocytes decreases gradually, and in 21 days after injection of the drug contents of the blast forms of the cells in the structural-functional zones of the lymph nodes does not differ from that in the control. The data obtained demonstrate the immunomorphological rearrangement of the lymph nodes in response to the exogenic lysozyme administration.
Shcherbakova
E G
EG
Kruglova
I S
IS
Larin
B A
BA
Zhuravleva
T P
TP
Rastunova
G A
GA
D000818:Animals; D002555:Cerebrospinal Fluid / Q000032:analysis / Q000187:drug effects; D004285:Dogs; D006651:Histocytochemistry; D007273:Injections, Intramuscular; D008196:Lymph / Q000032:analysis / Q000187:drug effects; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D009113:Muramidase / Q000032:analysis / Q000493:pharmacokinetics / Q000494:pharmacology*; D013997:Time Factors; D014018:Tissue Distribution
D009113:Muramidase
Morfotsitokhimicheskaia reaktsiia limfaticheskikh uzlov sobak na vvedenie lizotsima.