A stable colorimetric assay to measure toxin elevation of inorganic phosphate in bile.
A modified assay for the measurement of nanomole amounts of Pi in 50 microliters of bile is described. The assay is based on the formation of a complex between malachite green dye and phosphomolybdate under acidic conditions. Only three simple steps are required to produce a colored complex which remains stable for at least 3 h; these steps are precipitation with acid, addition of color reagent containing dye and surfactant, and incubation at 37 degrees C. Analysis of bile samples collected from a rat given the toxin 1,1-dichloroethylene demonstrated that the assay is well suited to routine assays of biliary Pi as an endogenous indicator of aberrant hepatobiliary function.
Moslen
M T
MT
Department of Pathology, University of Texas Medical Branch, Galveston.
Kanz
M F
MF
Ferguson
A E
AE
D000818:Animals; D001646:Bile / Q000032:analysis*; D004000:Dichloroethylenes / Q000494:pharmacology; D010710:Phosphates / Q000032:analysis*; D010743:Phospholipids / Q000494:pharmacology; D051381:Rats; D012394:Rosaniline Dyes; D013053:Spectrophotometry; D014118:Toxins, Biological / Q000494:pharmacology*
D004000:Dichloroethylenes; D010710:Phosphates; D010743:Phospholipids; D012394:Rosaniline Dyes; D014118:Toxins, Biological; C005095:malachite green