title: Regulation of actin polymerization by membrane fraction of platelets.
abstract: We studied the interaction between the purified membrane fraction of human platelets and the polymerization of skeletal actin. The viscosity of actin was measured by the falling ball method. The fraction suppressed the polymerization of actin in the presence of 20 mM KCl and 0.4 mM EGTA. The addition of calcium ion or thrombin to the fraction did not cause suppression. A DNase I affinity column bound the membrane fraction in the presence of calcium ion. The frozen membrane fraction and the vesicles reconstituted with lipids from the platelet membrane enhanced the polymerization of actin. Trypsinized membrane fraction and the membrane fraction treated with phospolipase A2 enhanced the polymerization of actin, but membrane fraction treated with phospholipase C had no effect. The reconstituted membrane vesicles mentioned above lowered the critical concentration for actin polymerization. These findings suggested that the polymerization of intracellular actin is enhanced not only by the mobilization of calcium ion, but also by biochemical changes in the membrane lipids.
authors: Department of Laboratory Medicine, Osaka City University Medical School, Japan.
authors: K
authors: K
authors: Hashimoto
authors: N
authors: N
authors: Tatsumi
mesh_terms: D000199:Actins / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002122:Calcium Chloride / Q000494:pharmacology; D002846:Chromatography, Affinity; D002855:Chromatography, Thin Layer; D006801:Humans; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000302:isolation & purification / Q000378:metabolism*; D008567:Membranes, Artificial; D010741:Phospholipases A / Q000494:pharmacology; D011108:Polymers; D013917:Thrombin / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000494:pharmacology
chemical_list: D000199:Actins; D008563:Membrane Lipids; D008567:Membranes, Artificial; D011108:Polymers; D010741:Phospholipases A; D010738:Type C Phospholipases; D014357:Trypsin; D013917:Thrombin; D002122:Calcium Chloride