title: Evidence for the occurrence of LDL receptors in extracts of schistosomula of Schistosoma mansoni.
abstract: Schistosomula of Schistosoma mansoni were shown to contain proteins on their surface membranes which bind iodinated human low density lipoproteins (125I-LDL). Treatment of the parasites with trypsin decreased the binding in comparison with untreated controls. Membrane-bound, acetone-insoluble proteins were extracted from the schistosomula with Triton X-100 and the extract in liposome form was incubated with 125I-LDL at room temperature. After incubation a complex was formed between the proteins present in the extract and 125I-LDL, as shown by a filter binding assay. 125I-LDL binding to filters was proportional to the amount of protein in the extract; it was inhibited by unlabelled LDL and VLDL and by EDTA. Binding of 125I-LDL to proteins present in the liposome suspension containing the Triton X-100 extract followed saturation kinetics, indicating the occurrence of receptors for lipoproteins in the extract.
authors: Departamento de Bioquímica, Universidade Federal de Minas Gerais, Pampulha, Belo Horizonte, Brazil.
authors: F D
authors: FD
authors: Rumjanek
authors: E G
authors: EG
authors: Campos
authors: L C
authors: LC
authors: Afonso
mesh_terms: D000818:Animals; D007700:Kinetics; D011973:Receptors, LDL / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D012550:Schistosoma mansoni / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D011973:Receptors, LDL; D014357:Trypsin