Effect of epidermal acylglucosylceramides and acylceramides on the morphology of liposomes prepared from stratum corneum lipids.
Epidermal acylglucosylceramides (AGC) and acylceramides (AC) cause aggregation and stacking of stratum corneum lipid liposomes formed from a lipid mixture containing epidermal ceramides (40%), cholesterol (25%), palmitic acid (25%), and cholesteryl sulfate (10%). This demonstrates the ability of these sphingolipids to hold adjacent bilayers in close apposition and their roles in the assembly of lamellar structures in the epidermis. However, AGC and AC in their hydrogenated form also caused aggregation and stacking of the stratum corneum lipid liposomes. This throws into doubt the proposed structural specificity of linoleate in the function of AGC and AC as molecular rivets in the assembly of the epidermal lamellar granules and the stratum corneum intercellular lamellae, respectively.
Abraham
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Marshall Dermatology Research Laboratories, Department of Dermatology, University of Iowa College of Medicine, Iowa City 52242.
Wertz
P W
PW
Downing
D T
DT
D000818:Animals; D002518:Ceramides; D002554:Cerebrosides / Q000494:pharmacology*; D002784:Cholesterol; D002788:Cholesterol Esters; D004817:Epidermis / Q000032:analysis*; D005614:Freeze Fracturing; D005963:Glucosylceramides / Q000494:pharmacology*; D008081:Liposomes; D008563:Membrane Lipids; D008854:Microscopy, Electron; D019308:Palmitic Acid; D010169:Palmitic Acids; D013107:Sphingolipids / Q000494:pharmacology*; D013552:Swine
D002518:Ceramides; D002554:Cerebrosides; D002788:Cholesterol Esters; D005963:Glucosylceramides; D008081:Liposomes; D008563:Membrane Lipids; D010169:Palmitic Acids; D013107:Sphingolipids; D019308:Palmitic Acid; D002784:Cholesterol; C007045:cholesteryl sulfate