title: Local effect of neurotensin on canine ileal blood flow, and its release by luminal lipid.
abstract: This study was performed to determine whether the level of neurotensin in mesenteric venous blood after lipid perfusion is sufficient to establish neurotensin as a mediator of lipid-induced mesenteric vasodilation. In anesthetized dogs, arterial flow to segments of the ileum was recorded, and blood was collected for measurement of neurotensin-like immunoreactivity, and neurotensin and metabolites. Perfusion of the lumen with micellar lipid resulted in an increase in blood blow from 37.7 +/- 4.1 to 44.5 +/- 3.9 ml/min/100 g (p less than 0.01; n = 8); flow to a control segment did not change. Venous plasma neurotensin-like immunoreactivity doubled, and neurotensin also increased (to 11.3 +/- 3.9 fmol/ml; p less than 0.05). Close intra-arterial infusion of neurotensin at 5 pmol/min increased blood flow to 44.3 +/- 3.4 ml/min/100 g (p less than 0.025; n = 5); flow to a control segment did not change. Neurotensin-like immunoreactivity increased to the same extent as with lipid perfusion, and neurotensin increased to 28.6 +/- 6.1 fmol/ml (p less than 0.05). No accumulation of metabolites was detected in either experiment. Thus, infused neurotensin caused increased ileal blood flow at a level in venous plasma comparable to that present after lipid perfusion, suggesting that neurotensin may have a role in the local regulation of ileal blood flow.
authors: Dept. of Medicine, Northwestern University Medical School Chicago, Illinois.
authors: R A
authors: RA
authors: Hammer
authors: B K
authors: BK
authors: Matsumoto
authors: A T
authors: AT
authors: Blei
authors: G
authors: G
authors: Pearl
authors: H
authors: H
authors: Ingram
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D007082:Ileum / Q000098:blood supply*; D007261:Infusions, Intra-Arterial; D008055:Lipids / Q000494:pharmacology*; D008297:Male; D009479:Neuropeptides / Q000097:blood; D009496:Neurotensin / Q000378:metabolism / Q000494:pharmacology*; D010477:Perfusion; D011863:Radioimmunoassay; D012039:Regional Blood Flow / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D008055:Lipids; D009479:Neuropeptides; C031644:neurotensin-like immunoreactivity; D009496:Neurotensin