[Assessment of the response of human bladder cancer cell lines to radiation using colony formation assay and [3H] thymidine incorporation assay].
The effect of radiation on the human bladder cancer cell lines, KK47 and J82, was studied using colony formation assay and [3H] thymidine incorporation assay. The response to radiation was dose-related for both assays, but the curve for the colony formation assay was steeper than that for [3H] thymidine incorporation assay. However, the steepness of the curve for [3H] thymidine incorporation assay increased to be approximately the same as that for the colony formation assay as the incubation period increased. [3H] Thymidine incorporation assay can provide a rapid assessment of radiation sensitivity comparable to that obtained by the colony formation assay to select a radiosensitive bladder cancer patient for definitive or adjunctive radiotherapy.
Kyu
L N
LN
Department of Urology, Faculty of Medicine, Kyoto University.
Kanamaru
H
H
Yoshida
O
O
D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D011836:Radiation Tolerance; D014410:Tumor Stem Cell Assay; D001749:Urinary Bladder Neoplasms / Q000503:physiopathology*
D004273:DNA, Neoplasm