[Electron microscopic study of Lassa virus].
Ultrafiltration through hollow fibrous filters followed by purification in interrupted and linear urografin gradients yielded a Lassa virus suspension of high concentration. The use of gamma-irradiation for inactivation of the frozen virus suspension (-70 degrees C) caused no apparent structural changes of virions and made it possible to examine Lassa virus in electron microscope by negative staining. The observed virus particles in their morphology and sizes did not differ from previously described particles of other members of the Arenaviridae family. In ultrathin sections of Lassa virus-infected Vero cells, atypical virions were sometimes visible alongside with typical particles. Within one type of such particles no ribosome-like granules could be detected. Such "hollow" particles may possibly be defective virions. Another kind of atypical particles contained homogeneous electron-dense core and resembled mycoplasma. Of greatest interest are the particles with heterogeneous core in which "sandy" granules can be distinguished. The presence of greater amounts of uranophilic material than usually may be explained by getting into the virion in the process of its formation of a greater amount of genetic material than that present in typical virions.
Il'kevich
Iu G
IuG
Lemeshko
N N
NN
Mar'iankova
R F
RF
Lukashevich
I S
IS
Klimenko
S M
SM
D000818:Animals; D001116:Arenaviridae / Q000648:ultrastructure*; D005720:Gamma Rays; D007836:Lassa virus / Q000302:isolation & purification / Q000528:radiation effects / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods; D013997:Time Factors; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods; D014709:Vero Cells; D014771:Virion / Q000302:isolation & purification / Q000528:radiation effects / Q000648:ultrastructure; D014775:Virus Activation / Q000528:radiation effects; D014776:Virus Cultivation
Elektronno-mikroskopicheskoe izuchenie virusa Lassa.