title: Positive correlation between cytosolic free calcium and surfactant secretion in cultured rat alveolar type II cells.
abstract: To determine whether increases in the cytosolic free Ca2+ concentration ([Ca2+]i) accompany agonist-stimulated surfactant secretion by cultured alveolar type II cells, we measured the [Ca2+]i of quin2-loaded cells isolated from adult rats before and after cells were stimulated with ionomycin, terbutaline or tetradecanoylphorbol acetate (TPA). To determine whether increases in [Ca2+]i are necessary for stimulated surfactant secretion to occur, we measured secretion in cells after [Ca2+]i had been reduced by loading cells with quin2 in medium containing low [Ca2+]. Ionomycin increased [Ca2+]i and stimulated surfactant secretion in a dose-dependent manner. Reductions in [Ca2+]i correlated with reductions in secretion stimulated by ionomycin, terbutaline or TPA. Ionomycin-stimulated secretion was most sensitive to reductions in [Ca2+]i; terbutaline-stimulated secretion was more sensitive than TPA-stimulated secretion. When [Ca2+]i was less than 65 nM, all stimulated secretion was blocked. Restoration of [Ca2+]i to greater than 100 nM restored ionomycin-stimulated secretion. We conclude that ionomycin increases [Ca2+]i and stimulates surfactant secretion in cultured alveolar type II cells, and that increased [Ca2+]i appears to be necessary for ionomycin-stimulated secretion to occur. Terbutaline-stimulated surfactant secretion seems to be more easily inhibited by a reduction in [Ca2+]i than does TPA-stimulated secretion.
authors: Cardiovascular Research Institute, University of California, San Francisco 94143.
authors: M S
authors: MS
authors: Pian
authors: L G
authors: LG
authors: Dobbs
authors: N
authors: N
authors: Düzgünes
mesh_terms: D000634:Aminoquinolines / Q000378:metabolism; D000818:Animals; D001572:Benzofurans / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D004987:Ethers / Q000494:pharmacology; D016257:Fura-2 / Q000031:analogs & derivatives*; D015759:Ionomycin; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013726:Terbutaline / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000634:Aminoquinolines; D001572:Benzofurans; D004987:Ethers; D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; C049925:fura-2-am; D015759:Ionomycin; D013726:Terbutaline; D013755:Tetradecanoylphorbol Acetate; C035739:Quin2; D002118:Calcium; D016257:Fura-2