Efficacy of an immunoglobulin preparation from HIV carriers in preventing HIV replication in vitro.
Immunoglobulin samples (HIV-Ig) were prepared by cold ethanol fractionation of human plasma containing antibody against human immunodeficiency virus (HIV). The ability to prevent viral spreading was studied using either human T-cell leukemia virus type I (HTLV-I)-carrying MT-4 cells or in a coculture system using MOLT-4 cells and virus-producing MOLT-4/HIV HTLV-IIIB cells. Treatment of HIV-infected MT-4 cells with HIV-Ig effectively blocked the appearance of antigens of HIV and the virus-induced cytopathic effect. HIV-Ig blocked multinucleated giant cell formation in the MOLT-4 and MOLT-4/HIV HTLV-IIIB coculture system.
Tochikura
T S
TS
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
Nakashima
H
H
Uemura
Y
Y
Goto
T
T
Suyama
T
T
Kobayashi
N
N
Yamamoto
N
N
D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D006678:HIV / Q000254:growth & development*; D006679:HIV Seropositivity / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral