Suprachiasmatic nucleus neurons immunoreactive for vasoactive intestinal polypeptide have synaptic contacts with axons immunoreactive for neuropeptide Y: an immunoelectron microscopic study in the rat.
An electron microscopic study showed by using a dual immunolabeling technique that in the suprachiasmatic nucleus of the rat, axon terminals immunoreactive for neuropeptide Y (NPY) made synaptic contacts upon neurons immunoreactive for vasoactive intestinal polypeptide (VIP). Diaminobenzidine (DAB)-labeled NPY axon terminals made synaptic contacts on silver-gold-labeled VIP perikarya and dendritic processes. The presynaptic NPY terminals contained many small clear vesicles and a few cored vesicles labeled with DAB chromogen. At the synaptic portion, a symmetrical thickening of the pre- and post-synaptic membranes was evident.
Hisano
S
S
Department of Anatomy, School of Medicine, University of Tokushima, Japan.
Chikamori-Aoyama
M
M
Katoh
S
S
Kagotani
Y
Y
Daikoku
S
S
Chihara
K
K
D000818:Animals; D001369:Axons / Q000032:analysis* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009478:Neuropeptide Y / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013493:Suprachiasmatic Nucleus / Q000032:analysis* / Q000648:ultrastructure; D013569:Synapses / Q000032:analysis* / Q000648:ultrastructure; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide