title: Cartilage proteoglycan aggregate and fibronectin can modulate the expression of type X collagen by embryonic chick chondrocytes cultured in collagen gels.
abstract: Chick embryo sternal chondrocytes from the caudal and cephalic regions were cultured within type I collagen gels and type I collagen/proteoglycan aggregate composite gels in normal serum. Caudal region chondrocytes were also cultured within type I collagen gels in the presence of fibronectin-depleted serum. There was a marked stimulation of type X collagen synthesis by the caudal region chondrocytes after 9 days in the presence of fibronectin-depleted serum and after 14 days in the presence of proteoglycan aggregate. These results provide evidence for the ability of chondrocytes from a zone of permanent cartilage to synthesise type X collagen and for the involvement of extracellular matrix components in the control of type X collagen gene expression.
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Biological Sciences, University of Manchester.
authors.forename: J T
authors.initials: JT
authors.lastname: Thomas
authors.forename: M E
authors.initials: ME
authors.lastname: Grant
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000502:physiology*; D005782:Gels; D005786:Gene Expression Regulation; D011509:Proteoglycans / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D003470:Culture Media; D005353:Fibronectins; D005782:Gels; D011509:Proteoglycans; D003094:Collagen