title: X chromosome duplications affect a region of the chromosome they do not duplicate in Caenorhabditis elegans.
abstract: X chromosome duplications have been used previously to vary the dose of specific regions of the X chromosome to study dosage compensation and sex determination in Caenorhabditis elegans. We show here that duplications suppress and X-linked hypomorphic mutation and elevate the level of activity of an X-linked enzyme, although these two genes are located in a region of the X chromosome that is not duplicated. The effects do not depend on the region of the X chromosome duplicated and is stronger in strains with two doses of a duplication than in strains with one dose. This is evidence for a general elevation of X-linked gene expression in strains carrying X-chromosome duplications, consistent with the hypothesis that the duplications titrate a repressor acting on many X-linked genes.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
authors.forename: P M
authors.initials: PM
authors.lastname: Meneely
authors.forename: K D
authors.initials: KD
authors.lastname: Nordstrom
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism; D000483:Alleles; D000818:Animals; D002107:Caenorhabditis / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D008297:Male; D009154:Mutation; D012732:Sex Determination Analysis; D014960:X Chromosome
chemical_list: D000110:Acetylcholinesterase