title: Transcription factor OTF-1 is functionally identical to the DNA replication factor NF-III.
abstract: Octamer transcription factor-1 (OTF-1) and nuclear factor III (NF-III) are sequence-specific DNA binding proteins that activate transcription and DNA replication, respectively. It is shown here that OTF-1 is physically and biologically indistinguishable from NF-III. This conclusion is based on the following observations. First, the two proteins have identical mobilities by SDS-polyacrylamide gel electrophoresis. Second, OTF-1 binds to the adenovirus origin of DNA replication at the same site and with the same affinity as NF-III. Third, OTF-1 can substitute for NF-III in activating the initiation of adenovirus DNA replication in vitro. Fourth, the ability of OTF-1 to stimulate viral DNA replication is dependent on the presence of an intact NF-III binding site within the origin of replication. Fifth, NF-III can substitute for OTF-1 in activating in vitro transcription from the human histone H2b promoter. These data suggest the possibility that NF-III/OTF-1 is a protein that functions in both cellular DNA replication and transcription.
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
authors.forename: E A
authors.initials: EA
authors.lastname: O'Neill
authors.forename: C
authors.initials: C
authors.lastname: Fletcher
authors.forename: C R
authors.initials: CR
authors.lastname: Burrow
authors.forename: N
authors.initials: N
authors.lastname: Heintz
authors.forename: R G
authors.initials: RG
authors.lastname: Roeder
authors.forename: T J
authors.initials: TJ
authors.lastname: Kelly
mesh_terms: D000256:Adenoviridae / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006657:Histones / Q000235:genetics; D051863:Host Cell Factor C1; D006801:Humans; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism / Q000494:pharmacology*; D050811:Octamer Transcription Factor-1; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D006657:Histones; D051863:Host Cell Factor C1; D009687:Nuclear Proteins; D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human; D014157:Transcription Factors