title: On the nature of IgG dimers. I. Dimers in human polyclonal IgG preparations: kinetic studies.
abstract: Human polyclonal IgG for therapeutic or prophylactic purposes is usually prepared from pooled plasmas taken from more than 1000 donors. After storage in solution under physiological conditions for a sufficiently long period, a considerable percentage of dimers (approx. 10-30% [w/w] at a protein concentration of approx. 160 mg/ml) represents the main component of aggregates in contrast to the essentially monomeric IgGs of monoclonal or single donor origin. Analysing the kinetics of monomer-dimer equilibration suggests assuming approx. 10(6) different antibody (ab) populations interacting independently and simultaneously with a specific partner of the reaction. Concerning the average apparent (functional) equilibrium constant of association, Kapp., a distinction could be made between two main populations characterized by values in the range if approx. 2.5-3.0 X 10(10) M-1 and 1.0 X 10(12) M-1, respectively. The results were obtained by computer simulation, taking an association rate constant, k+1, of 5 X 10(5) M-1 s-1 as a basis. Since the main part of the individual populations was found to interact Fc-independently via Fab-located binding sites, we suppose that the dimers are for the most part complexes of idiotypic (Ids) and anti-idiotypic abs (anti-Ids). Moreover, dimerization seems to be mainly a bivalent binding reaction, at least at the experimental concentrations. The results are in line with the concept of an idiotypic network regulation in man.
authors.affiliation: Research Laboratories of Behringwerke AG, Marburg, W. Germany.
authors.forename: P
authors.initials: P
authors.lastname: Gronski
authors.forename: R
authors.initials: R
authors.lastname: Bauer
authors.forename: L
authors.initials: L
authors.lastname: Bodenbender
authors.forename: E J
authors.initials: EJ
authors.lastname: Kanzy
authors.forename: K H
authors.initials: KH
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Zilg
authors.forename: F R
authors.initials: FR
authors.lastname: Seiler
mesh_terms: D000818:Animals; D001782:Blood Donors; D001797:Blood Protein Electrophoresis; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007700:Kinetics; D051379:Mice
chemical_list: D007074:Immunoglobulin G; C044411:polymeric IgG