Interactions in human casein systems: self-association of nonphosphorylated human beta-casein.
Since caseins were originally defined as phosphoproteins, nonphosphorylated beta-casein, comprising nearly 5% of the total beta-casein in the isoelectric precipitate from human milk, appears to be unique. Despite the relatively small amount present, its properties suggest that it may play an important role in micelle formation and structure. It has a partial specific volume, v, of 0.749 +/- 0.008 and an absorbance, E1% 1 cm,280 nm of 6.2 +/- 0.2. Sedimentation and viscosity data yield a solvation of 3 g H2O/g protein and an axial ratio of about 5 for the monomer. This would be consistent with a prolate ellipsoid of 10 nm length and 2 nm width. Equilibrium in the system is attained quite slowly and the temperature-dependent polymerization was found to be reversible. With calcium, the solubility behavior reflects an increased hydrophobicity and lower electrostatic repulsion in the molecule. There is essentially no strong calcium binding to this protein but there is evidence which strongly suggests that calcium binds to nonphosphate groups at higher concentrations. Increasing the temperature from 4 to 37 degrees C causes an apparent conformational change and an increase in protein aggregation which is further increased by addition of NaCl at 37 degrees C until a limiting size is reached at about 0.1 M NaCl. This limiting size polymer contains about 75 monomers and is nearly spherical with a radius of about 12 nm and a solvation of 1.5 g H2O/g protein. Laser light scattering measurements on the solution in 0.25 M NaCl revealed a relatively homogeneous particle size with a corrected diffusion coefficient, D20,w, of 2.8 X 10(-7) cm2/s.
Sood
S M
SM
Department of Biochemistry, Loma Linda University School of Medicine, California 92350.
Chang
P
P
Slattery
C W
CW
D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002364:Caseins / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D046911:Macromolecular Substances; D008895:Milk, Human / Q000032:analysis; D008970:Molecular Weight; D009994:Osmolar Concentration; D010766:Phosphorylation; D011506:Proteins; D012965:Sodium Chloride; D012995:Solubility; D012996:Solutions; D013053:Spectrophotometry; D013696:Temperature; D014461:Ultracentrifugation; D014783:Viscosity
D002364:Caseins; D046911:Macromolecular Substances; D011506:Proteins; D012996:Solutions; D012965:Sodium Chloride; D002118:Calcium