Single cell measurements in research on calcium-mobilising purinoceptors.
The Ca-sensitive photoprotein aequorin has been used to record repetitive free Ca transients in single rat liver cells responding to either ATP or ADP. The time-course of individual transients is longer when ATP is the agonist. If both agonists operate through the same receptor, then curtailment of the receptors' activity occurs more rapidly when ADP is the agonist, and we infer that an agonist-occupied receptor linked to a GTP-liganded G protein is the moiety responsible for activating a phosphatidylinositol bisphosphate-specific phospholipase C. The alternative explanation is that ATP and ADP act through different receptors.
Cobbold
P
P
Department of Human Anatomy and Cell Biology, University of Liverpool, U.K.
Woods
N
N
Wainwright
J
J
Cuthbertson
R
R
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000331:Aequorin / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008164:Luminescent Proteins / Q000494:pharmacology*; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism
D008164:Luminescent Proteins; D000331:Aequorin; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010738:Type C Phospholipases; D002118:Calcium