title: Thermolabile methylenetetrahydrofolate reductase in patients with coronary artery disease.
abstract: Thermostability of lymphocyte methylenetetrahydrofolate reductase (MTHFR) was determined in 21 patients aged less than 50 years with proven coronary artery disease, and in 21 age- and sex-matched controls without clinical evidence of vascular disease. The mean +/- SD of residual activity after heat inactivation at 46 degrees C for five minutes was 37.6% +/- 5.6% in the controls. In contrast, patients with coronary artery disease could be divided into two subgroups. Fifteen of them had 38.1 +/- 5.9% residual activity which was similar to that of the controls. In six of them the mean +/- SD residual activity after heat inactivation was 13.6% +/- 5.1% which was below 2 SD of the normal mean. These observations suggested that thermolabile MTHFR was associated with development of coronary artery disease.
authors.affiliation: Department of Pediatrics, Rush Medical College, Chicago, IL 60612.
authors.forename: S S
authors.initials: SS
authors.lastname: Kang
authors.forename: P W
authors.initials: PW
authors.lastname: Wong
authors.forename: J M
authors.initials: JM
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Sora
authors.forename: M
authors.initials: M
authors.lastname: Lessick
authors.forename: N
authors.initials: N
authors.lastname: Ruggie
authors.forename: G
authors.initials: G
authors.lastname: Grcevich
mesh_terms: D000328:Adult; D000367:Age Factors; D003327:Coronary Disease / Q000201:enzymology*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D008875:Middle Aged; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood*
chemical_list: D000587:Oxidoreductases Acting on CH-NH Group Donors; D042965:Methylenetetrahydrofolate Reductase (NADPH2)