Effects of estrone and estradiol-17 beta on 25-hydroxycholecalciferol hydroxylase activities in female Japanese quail.
Equimolar amounts of estrone and estradiol-17 beta injected into sexually immature female Japanese quail caused comparable increases and decreases in the renal activity of 25-hydroxycholecalciferol (25HCC) 1 alpha-hydroxylase and 25-hydroxycholecalciferol 24-hydroxylase, respectively. Peak activity of the former enzyme was induced by both estrogens within 6 hr and had declined by 24 hr. Plasma concentrations of estrone and estradiol following the injection were maximal within 3-6 hr of injecting the steroid i.m. Following [3H]estradiol injection, 25% of the radioactivity was located with estrone in the plasma following separation by TLC. Conversely, following [3H]estrone injection 30% of the radioactivity in plasma was located with estradiol. Thus, both estrone and estradiol may have physiological effects in quail, which in part may be due to their in vivo interconversion. Evidence that prolactin may be mediating the estrogenic stimulation of the 25HCC 1 alpha-hydroxylase is also presented.
Williams
P J
PJ
School of Biochemical and Physiological Sciences, University of Southampton, England.
Brain
P C
PC
Peddie
M J
MJ
Taylor
T G
TG
D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000097:blood; D003370:Coturnix / Q000378:metabolism* / Q000502:physiology; D003577:Cytochrome P-450 Enzyme System; D004958:Estradiol / Q000097:blood / Q000494:pharmacology*; D004970:Estrone / Q000097:blood / Q000494:pharmacology*; D005260:Female; D007668:Kidney / Q000201:enzymology*; D008297:Male; D011388:Prolactin / Q000378:metabolism; D011784:Quail / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*; D065668:Vitamin D3 24-Hydroxylase
D004970:Estrone; D004958:Estradiol; D011388:Prolactin; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D065668:Vitamin D3 24-Hydroxylase; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D002118:Calcium