Preparation and characterization of biotinylated psoralen.
Biotinylated psoralen (BPsor), a psoralen derivative containing a biotin moiety attached via a long-chain, positively charged linker, has been synthesized and its interactions with DNA and avidin have been studied. As do other psoralen derivatives, BPsor photoreacts with DNA to form interstrand crosslinks. The biotin binds to streptavidin after the reaction of BPsor with DNA, and this property has been used to measure low levels of BPsor modified DNA by ELISA with streptavidin and biotinylated alkaline phosphatase. In addition, BPsor retains the biological activity of psoralen, as shown by its ability to inhibit lymphocyte proliferation at a level of 10 ng/ml.
Saffran
W A
WA
Department of Genetics, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Welsh
J T
JT
Knobler
R M
RM
Gasparro
F P
FP
Cantor
C R
CR
Edelson
R L
RL
D001710:Biotin; D055598:Chemical Phenomena; D002621:Chemistry; D003432:Cross-Linking Reagents; D004247:DNA / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D010777:Photochemistry; D013057:Spectrum Analysis
D003432:Cross-Linking Reagents; D011564:Furocoumarins; D001710:Biotin; D004247:DNA