Simultaneous determination of D- and L-malic acids in apple juice by gas chromatography.
A gas chromatographic (GC) method was developed for the simultaneous determination of D- and L-malic acids in apple juice. D-Malic-(R)-(-)-2-butyl ester and L-malic-(R)-(-)-2-butyl ester are prepared with (R)-(-)-2-butanol. These diastereomers are separated and quantitated by gas chromatography on a Carbowax 20M (30 m x 0.25 mm) fused silica capillary column. This procedure does not involve any extraction or cleanup step.
Agarwal
V K
VK
Connecticut Agricultural Experiment Station, New Haven 06504.
D001628:Beverages / Q000032:analysis*; D002849:Chromatography, Gas; D005638:Fruit / Q000032:analysis*; D007202:Indicators and Reagents; D008293:Malates / Q000032:analysis*; D012997:Solvents; D013237:Stereoisomerism
D007202:Indicators and Reagents; D008293:Malates; D012997:Solvents; C030298:malic acid