Cell-cycle dependent transformation competence in Dictyostelium discoideum.
We describe a modification of the transformation procedure for Dictyostelium which allows for a more exact estimate of transformation efficiency and the isolation of primary transformants. Investigations of transformation competence revealed a negative correlation to cell density and a distinct distribution during the cell-cycle. In synchronized cells, transformation efficiency is 2-3 fold higher during mitosis when compared to unsynchronized cells.
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Max-Planck-Institut für Biochemie, Martinsried, FRG.
Saur
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U
D002453:Cell Cycle; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D007700:Kinetics; D014170:Transformation, Genetic