Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
Transcription of protein-encoding genes by human RNA polymerase II requires multiple ancillary proteins (transcription factors). Interactions between these proteins and the promoter DNA of a viral class II gene (the major late transcription unit of adenovirus) were investigated by enzymatic and chemical footprinting. The experiments indicated that the assembly of functionally active RNA polymerase II-containing transcription preinitiation complexes requires a complete set of transcription factors, and that both specific protein-DNA and protein-protein interactions are involved. This allows individual steps along the transcription reaction pathway to be tested directly, thus providing a basis for understanding basic transcription initiation mechanisms as well as the regulatory processes that act on them.
Van Dyke
M W
MW
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021.
Roeder
R G
RG
Sawadogo
M
M
D000260:Adenoviruses, Human / Q000235:genetics; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000502:physiology; D003851:Deoxyribonucleases / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II; D003851:Deoxyribonucleases