Occurrence of 3 beta-hydroxy-5-cholestenoic acid, 3 beta,7 alpha-dihydroxy-5-cholestenoic acid, and 7 alpha-hydroxy-3-oxo-4-cholestenoic acid as normal constituents in human blood.
Three unconjugated C27 bile acids were found in plasma from healthy humans. They were isolated by liquid-solid extraction and anion-exchange chromatography and were identified by gas-liquid chromatography-mass spectrometry, microchemical reactions, and ultraviolet spectroscopy as 3 beta-hydroxy-5-cholestenoic, 3 beta,7 alpha-dihydroxy-5-cholestenoic, and 7 alpha-hydroxy-3-oxo-4-cholestenoic acids. Their levels often exceeded those of the unconjugated C24 bile acids and the variations between individuals were smaller than for the C24 acids. The concentrations in plasma from 11 healthy subjects were 67.2 +/- 27.9 ng/ml (mean +/- SD) for 3 beta-hydroxy-5-cholestenoic acid, 38.9 +/- 25.6 ng/ml for 3 beta,7 alpha-dihydroxy-5-cholestenoic acid, and 81.7 +/- 27.9 ng/ml for 7 alpha-hydroxy-3-oxo-4-cholestenoic acid. The levels of the individual acids were positively correlated to each other and not to the levels of the C24 acids. The cholestenoic acids were below the detection limit (20-50 ng/ml) in bile and C27 bile acids present in bile were not detected in plasma.
Axelson
M
M
Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden.
Mörk
B
B
Sjövall
J
J
D000328:Adult; D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis; D005591:Chemical Fractionation; D002777:Cholestanols; D002782:Cholestenes / Q000097:blood*; D002783:Cholestenones / Q000097:blood*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000097:blood; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
C000986:3,7-dihydroxycholestan-26-oic acid; D001647:Bile Acids and Salts; D002777:Cholestanols; D002782:Cholestenes; D002783:Cholestenones; C056891:7 alpha-hydroxy-3-oxo-4-cholestenoic acid; C029435:3-hydroxy-5-cholestenoic acid; D002784:Cholesterol