Toxicity of n-C9 to n-C13 alkanes in the rat on short term inhalation.
Male Sprague Dawley rats were exposed to inhalation of n-C9 to n-C13 alkanes close to air saturation at 20 degrees (4438, 1369, 442, 142 and 41 p.p.m., respectively) for 8 hours and observed for the following 14 days. In addition, exposure to higher and lower concentrations of n-C9 was performed. The concentration of alkane in the brain after exposure exceeded that of blood for the lower alkanes, while the higher alkanes possessed a brain/blood ratio equal to or less than unity. Gross ataxia, general and focal seizure and spasms were observed in animals exposed to n-C9 in the range from 5280 to 3560 p.p.m. No toxic effects were observed in animals exposed to 2414 p.p.m. of n-C9 or to the other alkanes. An LC50 value for n-C9 of 4467 +/- 189 p.p.m. was estimated. Despite the clinical improvement in animals surviving the n-C9 exposure of 4438 p.p.m. (6/10), severe cerebellar damages were found at autopsy at the end of the observation period, with a loss of Purkinje cells as the most prominent feature. Immediate post mortem examination (4/10) showed marked vascular congestion of the liver as well as slight fatty degeneration but no cerebellar damage. No abnormalities were observed in animals exposed to the other alkanes. The significant distribution in the brain of the n-C9 alkane, the clinical signs of cerebellar dysfunction and the damage of cerebellar neurons would suggest CNS to be a possible target organ for the toxic effects of the n-C9 alkane.
Nilsen
O G
OG
Department of Pharmacology and Toxicology, University of Trondheim, Norway.
Haugen
O A
OA
Zahlsen
K
K
Halgunset
J
J
Helseth
A
A
Aarset
H
H
Eide
I
I
D000280:Administration, Inhalation; D000473:Alkanes / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000473:pathology; D007928:Lethal Dose 50; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000473:Alkanes