Release of NPY in pig pancreas: dual parasympathetic and sympathetic regulation.
Several lines of evidence have connected neuropeptide Y (NPY), a 36-residue polypeptide, to the sympathetic division of the autonomic nervous system. We studied the localization, the molecular characteristics, and the release of NPY and norepinephrine (NE) in the porcine pancreas. Immunohistochemical investigations revealed that NPY nerves around blood vessels were likely to be of adrenergic nature, whereas NPY-immunoreactive fibers close to exocrine and endocrine cells may originate from local ganglia also containing VIP (vasoactive intestinal peptide) and PHI (peptide histidine isoleucine). Electrical stimulation of the splanchnic nerve supply to the isolated perfused pig pancreas resulted in a corelease of NPY and NE into the venous effluent. Stimulation of the vagal nerves caused a sevenfold larger release of NPY without affecting the NE secretion. Characterization of the NPY immunoreactivity in the pancreatic tissue and in the venous effluent by gel filtration, high-performance liquid chromatography, and isoelectric focusing showed that the immunoreactive NPY was indistinguishable from synthetic porcine NPY. It is concluded that, although NPY is associated with sympathetic perivascular neurons, the majority of the pancreatic NPY-containing nerve fibers are likely to belong to the parasympathetic division of the autonomic nervous system.
Sheikh
S P
SP
Laboratory of Molecular Endocrinology, University Department of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark.
Holst
J J
JJ
Skak-Nielsen
T
T
Knigge
U
U
Warberg
J
J
Theodorsson-Norheim
E
E
Hökfelt
T
T
Lundberg
J M
JM
Schwartz
T W
TW
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004558:Electric Stimulation; D004594:Electrophysiology; D007150:Immunohistochemistry; D009478:Neuropeptide Y / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D010179:Pancreas / Q000294:innervation / Q000378:metabolism*; D010191:Pancreatic Polypeptide / Q000378:metabolism; D010275:Parasympathetic Nervous System / Q000502:physiology*; D013153:Splanchnic Nerves / Q000502:physiology; D013552:Swine; D013564:Sympathetic Nervous System / Q000502:physiology*; D014630:Vagus Nerve / Q000502:physiology
D009478:Neuropeptide Y; D010191:Pancreatic Polypeptide; D009638:Norepinephrine