title: Subchronic oral administration of gossypol-acetic acid (GAA) alters the distribution and utilization of radioiron in male rats.
abstract: Gossypol-acetic acid (GAA) has been shown to be a powerful chelator of iron. To determine how subchronic administration of GAA affects the distribution and utilization of radioiron, male Holtzman rats, which had received a daily oral dose of GAA (20 mg/kg body weight) for 16 weeks, were injected i.p. with [59Fe]citrate (activity = 16.5 muCi/kg body weight) and [59Fe] incorporation into circulating erythrocytes, bone marrow, liver, spleen, skeletal muscle, and urine was measured 6, 12, or 24 h after [59Fe] administration. Iron [59Fe] incorporation was enhanced in the spleens, livers, bone marrow, and blood of the GAA-treated rats, and increased as the time post-[59Fe] injection increased. Hematological parameters remained within normal limits throughout the 16-week treatment period, suggesting an erythropoietic compensation in the GAA-treated rats. Iron status was markedly different in the GAA-treated rats at the end of the 16 week treatment period, suggesting altered iron utilization. The data suggest that [59Fe] is preferentially distributed to the erythroid tissues of GAA-treated rats for utilization in erythropoiesis.
authors.affiliation: Department of Biological Sciences, Illinois State University, Normal 61761.
authors.forename: J M
authors.initials: JM
authors.lastname: Reynolds
authors.forename: J N
authors.initials: JN
authors.lastname: Tone
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001853:Bone Marrow / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D007501:Iron / Q000493:pharmacokinetics*; D007504:Iron Radioisotopes; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D013089:Spermatocidal Agents / Q000494:pharmacology*; D013154:Spleen / Q000378:metabolism
chemical_list: D007504:Iron Radioisotopes; D013089:Spermatocidal Agents; D007501:Iron; D006072:Gossypol; C028178:gossypol acetic acid