title: Synthesis of diadenosine 5',5'''-P1,P4-tetraphosphate by organellar and cytoplasmic phenylalanyl-tRNA synthetases of Euglena gracilis.
abstract: Purified phenylalanyl-tRNA synthetases present in chloroplasts, mitochondria and cytoplasm of green and bleached Euglena gracilis strains, respectively, are able to synthesize diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A). Ap4A synthesis is strictly dependent on zinc ions. This is the first evidence that chloroplasts should be able to synthesize Ap4A. Synthesis of Ap4A by phenylalanyl-tRNA synthetases of the three compartments of a plant cell or by other enzymes such as Ap4A phosphorylase is discussed.
authors.affiliation: Institut für Biochemie der Pflanzen der AdW der DDR, Halle/Saale.
authors.forename: R
authors.initials: R
authors.lastname: Krauspe
authors.forename: B
authors.initials: B
authors.lastname: Parthier
authors.forename: C
authors.initials: C
authors.lastname: Wasternack
mesh_terms: D000227:Adenine Nucleotides / Q000096:biosynthesis*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002736:Chloroplasts / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D015226:Dinucleoside Phosphates; D005055:Euglena / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D013431:Sulfates / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology; D019287:Zinc Sulfate
chemical_list: D000227:Adenine Nucleotides; D015226:Dinucleoside Phosphates; D013431:Sulfates; C020733:diadenosine tetraphosphate; D019287:Zinc Sulfate; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase; D015032:Zinc