Appearance of influenza A virus antigenic variants after treatment of infected MDCK cells with human leukocytes.
Exudation of polymorphonuclear leukocytes (PMN) from the infected mucosa is a characteristic feature of influenza virus infection. Since reactive oxygen species generated by PMN can be strong mutagens, the possibility of production of antigenic variants of the virus by virus-PMN interaction was investigated. Cloned influenza A NWS (H1N1) virus multiplying in Madin-Darby canine kidney cells was treated with human peripheral PMN. Assays in the presence and absence of monoclonal antibody to the cloned virus showed a seven- to ten-fold increase in the frequency of variants in the presence of PMN. The mutagenic effect was abolished by addition of superoxide dismutase to the culture.
Murakami
T
T
Department of Virology, Osaka City Institute of Public Health and Environmental Sciences, Japan.
Matsuyama
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D009154:Mutation; D009504:Neutrophils / Q000276:immunology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate