[Elimination of immune complex deposits from the kidneys of autoimmune NZB/N mice administered a xeno-spleen perfusate].
54 autoimmune NZB/N mice, 6 and 10 months of age, were intravenously injected 0.2 ml solution prepared during perfusion of the isolated sheep spleen with a buffer solution. Perfusion solution was injected 8 times with 2-3-day intervals. The control group of 43 mice received intravenously 0.2 ml of a buffer solution in the similar manner. After the treatment the levels of anti-DNA antibodies and circulating immune complexes were significantly decreased in the sera of mice which received the perfusion solution, as compared with the levels of control groups. Immunofluorescent studies showed a marked decrease in the number of glomerular immune complexes deposits in mice treated with perfusion solution. Six- and ten-month old mice exhibited a similar effect. The perfusion solution may be capable of eliminating the immune complexes from the blood and kidneys of autoimmune mice.
Tsypin
A B
AB
Nasonova
V A
VA
Vedernikova
L A
LA
Pleskovskaia
G N
GN
Beletskaia
L V
LV
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D007668:Kidney / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000628:therapy*; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D010477:Perfusion; D013154:Spleen / Q000276:immunology / Q000637:transplantation*; D014183:Transplantation, Heterologous
D000936:Antigen-Antibody Complex
Eliminatsiia otlozheniĭ immunnykh kompleksov iz pochek autoimmunnykh mysheĭ NZB/N pri vvedenii perfuzata ksenoselezenki.