The use of amniotic fluid and serum with propanediol in freezing of murine 2-cell embryos.
Human and mouse embryos have been cultured in amniotic fluid (AF). Human AF and human serum (HS) are used in the freeze-thaw of 2-cell mouse embryos. Two hundred seventy-five 2-cell embryos were collected into phosphate-buffered saline with 20% HS and 20% AF and into 100% HS and AF. The embryos were cooled with propanediol as cryoprotectant at a controlled rate. After thaw, they were cultured in T6 with 3 mg/ml bovine serum albumin. Blastocyst formation post-thaw was 56/79, 44/70, 51/61, and 56/79 of intact embryos from 20% HS, 20% AF, pure HS, and pure AF (NS). But blastocyst hatching was better from embryos frozen in pure HS (22/61, compared with 16/79 for 20% HS; P less than 0.05). Hence there is no advantage in using AF in freeze-thaw, but pure HS may be of use.
Ng
S C
SC
Department of Obstetrics and Gynaecology, National University Hospital, Singapore.
Sathananthan
H
H
Bongso
A
A
Lee
M N
MN
Mok
H
H
Wong
P C
PC
Ratnam
S S
SS
D000653:Amniotic Fluid; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D001769:Blood; D046508:Culture Techniques; D005260:Female; D005615:Freezing; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011409:Propylene Glycols; D012710:Serum Albumin, Bovine; D014021:Tissue Preservation
D011409:Propylene Glycols; D012710:Serum Albumin, Bovine