A fragile site in the human U2 small nuclear RNA gene cluster is revealed by adenovirus type 12 infection.
Using in situ hybridization, we found that the U2 small nuclear RNA gene cluster mapped very close to and was frequently disrupted by the gaps and breaks induced specifically in the human 17q21-q22 region by highly oncogenic adenovirus type 12 (Ad12). Restriction mapping revealed no structural alterations in the U2 gene locus as a result of Ad12 infection. Likewise, no Ad12-induced alterations in U2 RNA levels were detected. We estimate that the maximum size of the region specifically disrupted by this virus was less than 350 to 700 kilobases. A comparison of these data with similar data regarding biochemically induced fragile sites was made.
Durnam
D M
DM
Program in Experimental Pathology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Menninger
J C
JC
Chandler
S H
SH
Smith
P P
PP
McDougall
J K
JK
D000256:Adenoviridae / Q000502:physiology*; D002460:Cell Line; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure*; D004249:DNA Damage; D004622:Embryo, Mammalian; D006801:Humans; D008168:Lung; D012342:RNA, Small Nuclear / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D012342:RNA, Small Nuclear