A filtration assay for solubilized prolactin receptors using polyethyleneimine-treated membrane filters.
Free 125I-labeled ovine prolactin can be separated from detergent-solubilized prolactin-receptor complex by filtration on triacetate membrane filters pretreated with polyethyleneimine. Up to 98% of the total 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate solubilized prolactin-receptor complexes from rat liver bound to polyethyleneimine-treated membranes. This simple and rapid technique can be used to quantitate solubilized prolactin-receptor complexes.
el-Hamzawy
M A
MA
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Costlow
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ME
D000818:Animals; D003902:Detergents / Q000494:pharmacology; D005260:Female; D005374:Filtration / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000032:analysis; D008567:Membranes, Artificial; D011094:Polyethyleneimine; D011095:Polyethylenes; D051381:Rats; D011919:Rats, Inbred Strains; D011981:Receptors, Prolactin / Q000302:isolation & purification*; D012995:Solubility
D003902:Detergents; D008567:Membranes, Artificial; D011095:Polyethylenes; D011981:Receptors, Prolactin; D011094:Polyethyleneimine