Reversed-phase ion-pair chromatography of straight- and branched-chain dicarboxylic acids in urine as their 2-nitrophenylhydrazides.
The derivatization of urinary dicarboxylic acids with 2-nitrophenylhydrazine hydrochloride produced corresponding monohydrazides, which were separated from monocarboxylic acid hydrazides by two step extraction with ethyl acetate at different pH values. Monohydrazides of 11 straight- and branched-chain dicarboxylic acids were eluted isocratically on reversed-phase ion-pair chromatography within 24 min by the combination of pH, the polarity of mobile phase, and the size of counter ion. The analytical results showed good recovery and reproducibility using 3,3-dimethyglutaric acid as an internal standard. The present method provides a notable HPLC method with precolumn derivatization for the analysis of urinary dicarboxylic acids.
Miwa
H
H
Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Yamamoto
M
M
D002852:Chromatography, Ion Exchange / Q000379:methods; D003998:Dicarboxylic Acids / Q000652:urine*; D006801:Humans; D006834:Hydrazines / Q000652:urine*; D006863:Hydrogen-Ion Concentration; D009578:Nitrobenzenes / Q000652:urine*; D012997:Solvents
D003998:Dicarboxylic Acids; D006834:Hydrazines; D009578:Nitrobenzenes; D012997:Solvents