title: Electroporation-mediated transfection of Acholeplasma laidlawii with mycoplasma virus L1 and L3 DNA.
abstract: In contrast to mycoplasma virus L1 and L2 circular DNA, mycoplasma virus L3 linear DNA is not biologically active in polyethylene glycol-mediated transfection. Electroporation of Acholeplasma laidlawii, however, leads to plaque formation after incubation with L3 DNA. The efficiency of electroporation-mediated transfection is 1/10 that of polyethylene glycol-mediated transfection as estimated with L1 DNA. Trypsin treatment of cells before DNA addition increases the efficiency of DNA uptake.
authors.affiliation: Abteilung Virologie, Institut für Mikrobiologie, Universität Ulm, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Lorenz
authors.forename: W
authors.initials: W
authors.lastname: Just
authors.forename: M
authors.initials: M
authors.lastname: da Silva Cardoso
authors.forename: G
authors.initials: G
authors.lastname: Klotz
mesh_terms: D000128:Acholeplasma laidlawii / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D004560:Electricity; D008565:Membrane Proteins / Q000378:metabolism; D014162:Transfection; D014357:Trypsin / Q000378:metabolism
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D008565:Membrane Proteins; D014357:Trypsin