Characterization of the genes for fructose-bisphosphate aldolase in Trypanosoma brucei.
In Trypanosoma brucei stock 427 the glycolytic enzyme fructose-bisphosphate aldolase is encoded by two tandemly linked genes of identical sequence. Such a tandem arrangement of aldolase genes is also present in other T. brucei stocks of unrelated origin. In stock 427 one of the allelic genes is a pseudogene, as a result of a one-nucleotide deletion. The genes code for a polypeptide of 371 amino acids, with a calculated molecular weight of 40,940. The protein that is predicted from the gene sequence has 45-48% positional identity with known aldolase sequences of other organisms. The trypanosomal protein is, however, unique in having a 10 amino-acid insertion near its N-terminus and high number of basic residues, a feature it shares with other glycolytic enzymes of T. brucei. These glycolytic enzymes have in common that they are located in microbody-like organelles, the glycosomes. We have previously proposed that the positively charged residues may be involved in the import of the proteins into the organelles.
Marchand
M
M
International Institute of Cellular and Molecular Pathology, Research Unit for Tropical Diseases, Brussels, Belgium.
Poliszczak
A
A
Gibson
W C
WC
Wierenga
R K
RK
Opperdoes
F R
FR
Michels
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics*
D004247:DNA; D005634:Fructose-Bisphosphate Aldolase