H-n.m.r. studies on the specificity of the interaction between bovine pancreatic ribonuclease A and dideoxynucleoside monophosphates.
The titration curves of the C-2 histidine protons of bovine pancreatic ribonuclease A in the presence of several dideoxynucleoside monophosphates (dNpdN) were studied by means of proton nuclear magnetic resonance at 270 MHz in order to obtain information on the ligand--RNase A interaction. The changes in the chemical shift and pKs of the C-2 proton resonances of His-12, -48, -119 in the complexes RNase A--dNpdN were smaller than those previously found when the enzyme interacted with mononucleotides. The pK2 of His-12 was not affected by the interaction of the enzyme with these ligands, whereas, the perturbation of the pK2 of His-119 was clearly dependent on the nature of the ligand. If there is a pyrimidine nucleoside at the 3' side of the dideoxynucleoside monophosphates, as in TpdA and TpT, an enhancement due to the well known interaction of the phosphate in p1, the catalytic site, was found. However, when there is a purine nucleoside, as in dApT and dApdA, a decrease in the pK2 value was observed and we propose that in such cases the phosphate group interacts in a secondary phosphate binding site, p2. The results obtained suggest the existence of different specific interactions depending on the structure of the dideoxynucleoside monophosphate studied.
Alonso
J
J
Department of Biochemistry and Molecular Biology, Faculty of Sciences, Free University of Barcelona, Bellaterra, Spain.
Paolillo
L
L
D'Auria
G
G
Nogués
M V
MV
Cuchillo
C M
CM
D003854:Deoxyribonucleotides / Q000378:metabolism*; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011485:Protein Binding; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*; D013329:Structure-Activity Relationship
D003854:Deoxyribonucleotides; D008024:Ligands; D006859:Hydrogen; D012259:Ribonuclease, Pancreatic