[Kinetics of protein degradation in diploid and trisomic human fibroblasts].
The degradation rate of long-lived and short-lived proteins was determined in diploid fibroblasts and fibroblasts with trisomy 7 derived from human embryos. Two fractions of proteins were detected in the exponentially growing diploid fibroblasts with half-lives (T 1/2) 37 and 19 hours. The rate of protein degradation increases in diploid fibroblasts as they approach confluence and protein fractions with T 1/2 30, 18 and 12 hours appear. The rate of protein degradation in trisomic fibroblasts does not change for the long-lived and short-lived proteins and is the same in both exponential (T 1/2 31 and 14 hours) and stationary phase (T 1/2 33 and 17 hours). The relative amount of the short-lived proteins in trisomic fibroblasts in the stationary phase decreased as compared with the one in diploid fibroblasts. It is apparent that a mechanism of regulation of protein catabolism in trisomic fibroblasts is impaired.
Podobed
O V
OV
Golubeva
N V
NV
Kukharenko
V I
VI
Del'vig
A A
AA
D002478:Cells, Cultured; D004171:Diploidy; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D011506:Proteins / Q000378:metabolism*; D014314:Trisomy
D011506:Proteins
Kinetika raspada belkov v diploidnykh i trisomnykh fibroblastakh cheloveka.