Idazoxan blocks the action of noradrenaline but not spinal inhibition from electrical stimulation of the locus coeruleus and nucleus Kolliker-Fuse of the cat.
The effects of idazoxan, a specific alpha 2-adrenoceptor antagonist, on spinal inhibition by administration of noradrenaline and from electrical stimulation of the dorsolateral pons were studied in 26 cats anaesthetized with sodium pentobarbitone. The excitation of dorsal horn neurons of the spinal cord by noxious heating of the skin or by impulses in unmyelinated primary afferent fibres was markedly reduced when noradrenaline was administered microelectrophoretically in the substantia gelatinosa or near cell bodies. Electrical stimulation of the region of the locus coeruleus selectively inhibited spinal nociceptive transmission when dorsal horn neurons were excited by noxious and non-noxious stimuli. In contrast to stimulation of the locus coeruleus, stimulation of the nucleus Kolliker-Fuse produced non-selective inhibition of both nociceptive and non-nociceptive responses of dorsal horn neurons of the spinal cord. Microelectrophoretic ejection of idazoxan reduced or abolished noradrenaline-induced inhibition with 19 of 20 neurons. This antagonist did not alter inhibition from stimulation of the locus coeruleus and the nucleus Kolliker-Fuse, regardless of whether it was administered microelectrophoretically (11 neurons), systemically (3 neurons) or topically (4 neurons). The results suggest that alpha 2-adrenoceptors do not mediate inhibition of spinal nociceptive transmission from electrical stimulation of the locus coeruleus and the nucleus Kolliker-Fuse. Other possibilities for the failure of idazoxan to modify inhibition from such stimulation are discussed.
Zhao
Z Q
ZQ
Department of Pharmacology, John Curtin School of Medical Research, Australian National University, Canberra.
Duggan
A W
AW
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002415:Cats; D003000:Clonidine / Q000494:pharmacology; D004146:Dioxanes / Q000494:pharmacology*; D004147:Dioxins / Q000494:pharmacology*; D004558:Electric Stimulation; D019329:Idazoxan; D008125:Locus Coeruleus / Q000502:physiology*; D009433:Neural Inhibition; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology*; D010146:Pain / Q000503:physiopathology*; D010812:Physical Stimulation; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*
D004146:Dioxanes; D004147:Dioxins; D003000:Clonidine; D009638:Norepinephrine; D019329:Idazoxan