title: Pancreatic polyamine concentrations and cholecystokinin plasma levels in rats after feeding raw or heat-inactivated soybean flour.
abstract: We investigated the trophic effect on the pancreas of male Wistar rats fed up to 20 days with either raw soybean flour (RSF) containing an active trypsin inhibitor or heat-inactivated soybean flour (HSF). The concentrations of the polyamines putrescine, spermidine, and spermine in the pancreas as well as cholecystokinin (CCK) concentrations in arterial and portal vein plasma were measured. Plasma CCK concentrations were measured by a sensitive radioimmunoassay specific for the sulfated region of CCK, whereas polyamine concentrations are determined by reversed phase high-performance liquid chromatography. The levels of CCK in both arterial and portal vein plasma were significantly higher in RSF- compared with HSF-fed rats, the concentration in the portal vein being twice as high compared with the aorta. A significant increase in pancreatic weight and protein content was positively correlated to an increase in putrescine and spermidine in the pancreas of RSF-fed rats compared with HSF-fed controls, whereas the spermine content did not differ between the two groups. The pancreatic DNA content in RSF-fed rats was significantly above control values of day 20 only. These data support the hypothesis that the trophic effect of soybean trypsin inhibitor on the pancreas is mediated by CCK and that polyamines might play an important role in CCK-induced pancreatic growth.
authors.affiliation: Department of Medicine, Georg-August-University of Göttingen, F.R.G.
authors.forename: C
authors.initials: C
authors.lastname: Löser
authors.forename: U R
authors.initials: UR
authors.lastname: Fölsch
authors.forename: D
authors.initials: D
authors.lastname: Mustroph
authors.forename: P
authors.initials: P
authors.lastname: Cantor
authors.forename: U
authors.initials: U
authors.lastname: Wunderlich
authors.forename: W
authors.initials: W
authors.lastname: Creutzfeldt
mesh_terms: D000818:Animals; D002766:Cholecystokinin / Q000097:blood*; D004247:DNA / Q000378:metabolism; D006358:Hot Temperature; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D011073:Polyamines / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013025:Glycine max / Q000633:toxicity*; D014361:Trypsin Inhibitors / Q000633:toxicity
chemical_list: D011073:Polyamines; D011506:Proteins; D014361:Trypsin Inhibitors; D004247:DNA; D002766:Cholecystokinin