Differences in the susceptibility of human blood cell lines to vaccinia virus.
The replication of vaccinia virus in human hematopoietic cell lines was studied, to ascertain whether the cell type and the stage of differentiation can influence the outcome of the infection. Lymphocytic NB104 cells and myelogenous leukemic K562 cells can be productively infected with vaccinia. Whereas NB104 cells were readily lysed, cells from the K562 line stayed persistently infected. Infection of promyelocytic leukemia HL60 cells results in an abortive infection in which the virus is uncoated, does not replicate, remains latent and can be rescued by cocultivation with permissive cells. Upon addition of phorbol esters to HL60 cell cultures, viral replication resumed. These results suggests that, although vaccinia virus replication is almost independent of host-specific functions, the outcome of the infection may depend on the cell type and its stage of differentiation.
Pogo
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BG
Center for Experimental Cell Biology, Mollie B. Roth Laboratory, Mount Sinai School of Medicine, City University of New York, New York 10029-6574.
Lai
A C
AC
Holland
J G
JG
Friend
C
C
D002454:Cell Differentiation; D002460:Cell Line / Q000382:microbiology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D007962:Leukocytes / Q000382:microbiology*; D008214:Lymphocytes / Q000382:microbiology*; D012367:RNA, Viral / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014616:Vaccinia virus / Q000254:growth & development / Q000472:pathogenicity*; D014764:Viral Proteins / Q000096:biosynthesis; D014775:Virus Activation; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide