Efficient malignant transformation of rat embryo fibroblasts by genomic DNA from Walker carcinoma cells.
DNA isolated from Walker carcinoma ascites cells was transfected into primary rat embryo fibroblasts (REF), selecting transformed cells by growth in soft agar after prolonged propagation in monolayer. Both high molecular weight genomic DNA and a partially purified mitochondrial DNA fraction were able to transform REF with high efficiency, whereas pure mitochondrial DNA failed to elicit a transformed phenotype. Hybridization experiments showed that the mitochondrial DNA fraction contained DNA species of presumably extramitochondrial origin. Colonies were cloned into morphologically transformed, foci-forming, immortalized cell lines, showing different degrees of chromosomal alterations, tumorigenicity, and production of cell growth factors. These results indicate that although REF are refractory to genomic neoplastic DNA or to single cloned oncogenes in the absence of enhancers, they can be efficiently transformed by chromosomal DNA from a highly malignant tumor under conditions selecting against the remaining normal cells.
Arvelo
F
F
Centro de Biologia Celular, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.
Perez
J L
JL
Antuna
O
O
Gonzalez-Cadavid
N F
NF
D000818:Animals; D002279:Carcinoma 256, Walker / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000209:etiology*; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000235:genetics*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000473:pathology; D051381:Rats; D014162:Transfection
D004273:DNA, Neoplasm