title: Transmission of the Ca2+-regulatory signal in skeletal muscle thin filaments.
abstract: Studies dealing with some aspects of the thin-filament based regulatory mechanism of striated muscle are discussed. A key event is a Ca2+-induced shift of TnI from a binding site on actin to TnC, as indicated by optical and crosslinking experiments. The regions concerned involve residues 1-12 in actin, residues 96-116 in TnI, and residues 89-100 in TnC. These changes in interaction are accompanied by changes in TnC and TnI that occur on a millisecond time scale which is consistent with their being part of the regulatory processes underlying regulation in vivo.
authors.affiliation: Department of Muscle Research, Boston Biomedical Research Institute, Massachusetts.
authors.forename: J
authors.initials: J
authors.lastname: Gergely
authors.forename: Z
authors.initials: Z
authors.lastname: Grabarek
authors.forename: P C
authors.initials: PC
authors.lastname: Leavis
authors.forename: G
authors.initials: G
authors.lastname: Strasburg
authors.forename: T
authors.initials: T
authors.lastname: Tao
authors.forename: C L
authors.initials: CL
authors.lastname: Wang
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology*; D000199:Actins / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology*; D004735:Energy Transfer; D007700:Kinetics; D009132:Muscles / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D014336:Troponin / Q000378:metabolism
chemical_list: D000199:Actins; D014336:Troponin; D002118:Calcium