Effect of sodium-ascorbate and vanadate on the Rb+-uptake of human red blood cells.
To follow the Rb+-uptake of human red blood cells (rbc-s) under different circumstances, a micro-method was developed. According to our experiments the Rb+-uptake of rbc-s in a healthy person was about 3.5-4.0 mumoles Rb/mg Fe at 37 degrees C during 120 minutes. When red cells were incubated with solutions containing different concentrations, between 15 and 120 mmole/l, of Na-ascorbate [Na-ascorbate was applied at the expense of Na-isethionate (sodium salt of 2-hydroxyethan-1-sulfonic acid)] the Rb+-uptake of red cells increased at 37 degrees C with 37 to 70% respectively. In other experiments it was established that the ouabain-sensitive Rb+-uptake of rbc-s decreased with 50% in the presence of 0.1 mmole/l vanadate, while if Na-ascorbate was applied simultaneously with different concentrations of vanadate, or after a preincubation with 1 mmole/l vanadate, the Rb+-uptake of red cells, which had been reduced by vanadate, returned close to that of control. This effect can be explained by the reductive property of Na-ascorbate, i.e. by the transformation of vanadate to vanadil.
Eliás
G
G
Central Research Laboratory, University Medical School, Debrecen, Hungary.
Juhász
A
A
Jeney
F
F
Kövér
A
A
D001205:Ascorbic Acid / Q000494:pharmacology*; D004347:Drug Interactions; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D012413:Rubidium / Q000097:blood*; D014638:Vanadates / Q000097:blood / Q000494:pharmacology*
D014638:Vanadates; D012413:Rubidium; D001205:Ascorbic Acid