Chronic ethanol exposure in the embryonic chick heart: effect on myocardial function and structure.
To investigate the effect of chronic ethanol exposure on the embryonic chick heart, chick embryos were exposed daily to one of seven graded doses of ethanol or to saline only (shams) from 0 to 96 hr of incubation. One hour before and after exposure at 72 hr, and 1 hr before and after exposure at 96 hr, embryos were analyzed for changes in heart function, embryo tissue ethanol content, occurrence of anomalies, and embryo weights. At both 71 and 73 hr of incubation (during cardiogenesis), when compared to shams, heart rate (HR) in embryos receiving ethanol doses greater than 0.0375 ml increased significantly (P less than .05) with commensurate increases in injected ethanol. Additionally, at 73 hr, depressed cardiac contractility, measured as shortening fraction, was noted at doses greater than or equal to .0375 when compared to shams. While slight increases in shortening fraction (SF) across dose were noted at 95 and 97 hr, only random doses were statistically significant from shams, with no specific trend in either HR or SF at this postcardiogenesis stage. Within each time group, gas chromatography analysis of embryo tissue ethanol content demonstrated a linear relationship between dose injected and tissue ethanol content retrieved. With increasing dose and stage, viability decreased. Weights of ethanol-injected embryos were not significantly different from shams within each time group. Our studies of the response of the embryonic chick heart to ethanol indicate both dose and stage susceptibility, with greater susceptibility to ethanol injury during active cardiogenesis.
Ruckman
R N
RN
Department of Cardiology, Children's Hospital National Medical Center, Washington, D.C. 20010.
Messersmith
D J
DJ
O'Brien
S A
SA
Getson
P R
PR
Boeckx
R L
RL
Morse
D E
DE
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002642:Chick Embryo; D002849:Chromatography, Gas; D000431:Ethanol / Q000633:toxicity*; D006321:Heart / Q000187:drug effects* / Q000196:embryology; D006339:Heart Rate / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects
D000431:Ethanol