A new quantitative spectrophotometric assay of ischemia-reperfusion injury in skeletal muscle.
Characterization of ischemia-reperfusion injury of skeletal muscle remains poorly defined. A new quantitative assay to measure ischemic skeletal muscle injury is described and validated in a rat hindlimb model. This biochemical spectrophotometric technique measures triphenyltetrazolium chloride reduction in ischemic muscle. The reduction assay demonstrated significant injury after 3 hours of ischemia (25.4 +/- 9.7 percent of control activity; p less than 0.05). More severe injury occurred after 4 or more hours (less than 3 percent of control activity; p less than 0.05). This assay is an objective and quantitative method for characterizing ischemia-reperfusion injury.
Belkin
M
M
Department of Surgical Research, Boston University School of Medicine, Massachusetts.
Brown
R D
RD
Wright
J G
JG
LaMorte
W W
WW
Hobson
R W
RW
D000818:Animals; D006614:Hindlimb; D006801:Humans; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000098:blood supply*; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D013053:Spectrophotometry; D013778:Tetrazolium Salts
D013778:Tetrazolium Salts; C009591:triphenyltetrazolium