Use of a single-injection tissue-sampling technique to study steroid uptake by the decidualized endometrium of the mouse.
A tissue-sampling paired-tracer method was used to investigate the effect of plasma proteins on uptake by the decidualized endometrium of [3H]progesterone, [3H]oestradiol and [3H]corticosterone. When injected arterially in protein-free Ringer, the extraction of progesterone and oestradiol was 100%, while that of corticosterone was only 60%. The addition of 4% albumin or injection in mouse plasma resulted in significant decreases in progesterone extraction to about 80% and 65% respectively. Injection in pregnant guinea-pig plasma reduced progesterone extraction further (to 33%). While neither 4% albumin nor mouse plasma had any significant effect on the uptake of oestradiol, neonatal rat plasma reduced oestradiol extraction to 40%. These results are consistent with high-affinity binding proteins having a limiting effect on the availability of steroids to target tissues.
Edwards
C
C
Department of Physiology, King's College, Strand, London, U.K.
Milligan
S R
SR
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D003345:Corticosterone / Q000378:metabolism*; D003656:Decidua / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007444:Inulin / Q000378:metabolism; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D010052:Ovariectomy; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*
D001798:Blood Proteins; D011374:Progesterone; D004958:Estradiol; D007444:Inulin; D003345:Corticosterone