title: Antibodies labeled with 199Au: potential of 199Au for radioimmunotherapy.
abstract: We have investigated the direct labeling of antibodies with gold isotopes as an alternative to iodine and chelated metals for antibody guided therapy. Both 199Au and 195Au complex anions in citrate-buffered solutions (pH 3.8-7.2) are rapidly bound to sheep polyclonal anti-CEA with high efficiency. After purification by gel filtration, the antibody retained 80% of the bound gold for upto 6 days at 4 degrees C and was reactive with its target antigen in a solid phase assay. Incubation with the gold binding substance D-penicillamine reduced labeling efficiency and stability.
authors.affiliation: Department of Immunology, University of Birmingham Medical School, U.K.
authors.forename: P
authors.initials: P
authors.lastname: Anderson
authors.forename: A T
authors.initials: AT
authors.lastname: Vaughan
authors.forename: N R
authors.initials: NR
authors.lastname: Varley
mesh_terms: D000818:Animals; D000906:Antibodies; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002850:Chromatography, Gel; D006050:Gold Radioisotopes / Q000627:therapeutic use*; D007474:Ion Exchange; D007553:Isotope Labeling; D012756:Sheep
chemical_list: D000906:Antibodies; D002272:Carcinoembryonic Antigen; D006050:Gold Radioisotopes