title: Ferredoxin and cytochrome P-450scc concentrations in granulosa cells of porcine ovaries during follicular cell growth and luteinization.
abstract: The concentrations of cytochrome P-450scc and ferredoxin, two of the three proteins which comprise the mitochondrial steroidogenic electron transport chain, were measured in granulosa and luteal cells from porcine ovaries by an immunoblot procedure. During the follicular phase of the ovarian cycle the concentration of cytochrome P-450scc increased 5-fold and ferredoxin increased 3-fold. When the large follicles developed into corpora lutea the cytochrome P-450scc concentration increased a further 7-fold while ferredoxin increased only 3-fold. These changes were coincident with an overall 4-fold increase in the concentration of ferredoxin reductase during follicular cell development and luteinization. Analysis of the data revealed that the concentration of ferredoxin, which shuttles electrons from ferredoxin reductase to cytochrome P-450scc, was always adequate to saturate both the reductase and cytochrome P-450scc. This came about from a co-ordinate increase in the concentration of cytochrome P-450scc and the concentration of ferredoxin minus ferredoxin reductase.
authors.affiliation: Department of Biochemistry, University of Western Australia, Perth.
authors.forename: R C
authors.initials: RC
authors.lastname: Tuckey
authors.forename: Z
authors.initials: Z
authors.lastname: Kostadinovic
authors.forename: P M
authors.initials: PM
authors.lastname: Stevenson
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000276:immunology / Q000378:metabolism*; D003338:Corpus Luteum / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism; D005288:Ferredoxins / Q000276:immunology / Q000378:metabolism*; D006107:Granulosa Cells / Q000502:physiology*; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D008184:Luteal Cells / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*; D013552:Swine
chemical_list: D005288:Ferredoxins; D007106:Immune Sera; D002786:Cholesterol Side-Chain Cleavage Enzyme; D005287:Ferredoxin-NADP Reductase