A semiautomated system for measurement of 96 simultaneous spectrophotometric enzyme assays.
A semiautomated system for spectrophotometric measurement of enzyme activity is described. In comparison to a 1-ml reaction volume monitored continuously by a conventional spectrophotometer, this system requires 1/10 to 1/100 the volume of sample, and 1/8 to 1/4 the time for measurement and computation of 96 enzyme assays. The system hardware consists of a 96-well platereader interfaced to a personal computer. Absorbances of 96 reactions are measured at timed intervals. These data are transmitted electronically from the platereader to the computer through the modem port using a modem program. The reaction rates are computed from the timed absorbance readings using a spreadsheet program. Three enzyme assays are presented, but the method has been used for several other assays and is applicable to many spectrophotometric rate assays. Many laboratories currently possess one or both of the two major components of the relatively inexpensive system described.
Butler
E G
EG
Division of Pathology and Toxicology, American Health Foundation, Valhalla, New York 10595.
Gill
R
R
Garych
L
L
D000818:Animals; D002802:Cholinesterases / Q000032:analysis; D003201:Computers; D004798:Enzymes / Q000032:analysis*; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000201:enzymology; D051381:Rats; D012984:Software; D013053:Spectrophotometry
D004798:Enzymes; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D002802:Cholinesterases