title: A rapid computerized method for quantitation of cellular mediated immunity in biological systems.
abstract: The aim of this study was to compare the quantitation of cellular mediated immunity (CMI) assessed by leukocyte migration (LM) using a Hewlett Packard Model 9825A Desk-top computer interfaced to a Talos Cyber Graphics Digitizer vs. the standard quantitation done by tracing the projected profile of the migrated leukocytes on papers, cutting and weighing. The computerized quantitation resulted in a percentage migration mean of 75.91 as compared to standard quantitation by weighing (76.37) (P greater than 0.05). The unweighted least squares linear regression resulted in R2 0.9987, an indication of a high correlation between the data obtained by both methods. The advantages of computerized quantitation of cellular mediated immunity are discussed.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
authors.forename: E K
authors.initials: EK
authors.lastname: Barbour
authors.forename: J A
authors.initials: JA
authors.lastname: Newman
authors.forename: J
authors.initials: J
authors.lastname: Sasipreeyajan
mesh_terms: D000818:Animals; D002465:Cell Movement; D002645:Chickens; D007962:Leukocytes / Q000276:immunology*; D008432:Mathematical Computing; D008838:Microcomputers; D012984:Software