title: HPLC determination of 5-hydroxypropafenone in plasma with electrochemical detection.
abstract: A high pressure liquid chromatographic (HPLC) method with internal analogue standardization for the determination of 5-hydroxypropafenone in plasma is described. The method comprises extraction from plasma at pH 9 with diethyl-ether and quantification after HPLC separation using a reverse phase by means of electrochemical (reductive) detection after electrochemical preoxidation. When using 1 ml plasma the lower limit of detection is 0.5 ng/ml. Under routine conditions the limit of determination is estimated to be lower than 2 ng/ml. The variation coefficients of duplicates drop from about 10% in the range of the determination limit to about 3% at 5 ng/ml and above. The determination of 5-hydroxypropafenone is not impaired by other known metabolites of propafenone.
authors.affiliation: Knoll AG, Dept. Biochemistry, Ludwigshafen/Rhein, FRG.
authors.forename: E
authors.initials: E
authors.lastname: Brode
authors.forename: U
authors.initials: U
authors.lastname: Kripp
authors.forename: W
authors.initials: W
authors.lastname: Breckwoldt
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011405:Propafenone / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D011405:Propafenone; C044307:5-hydroxypropafenone