title: Determination of 2,3-dihydro-6-[3-(2-hydroxymethyl)phenyl-2-propenyl]-5-benzofuranol in plasma using liquid chromatography with electrochemical detection.
abstract: A reversed-phase column liquid chromatographic (LC) method with electrochemical detection (ED) is described for the quantification of 2,3-dihydro-6-[3-(2-hydroxymethyl)phenyl-2-propenyl]-5-benzofuranol (compound 1), a new locally active dual inhibitor of leukotriene and prostaglandin synthesis, in plasma. After a single liquid-liquid extraction of the biological specimen, the extract was analyzed using a liquid chromatograph with an amperometric detector set at an oxidation potential of +0.55 V. The resulting chromatograms are free from endogenous interference and the limit of detection is 0.2 ng/ml. Several other analogous dihydrobenzofuranols were shown to be electrochemically active, permitting their determination using LC with ED. The described analytical method has been fully validated in the concentration range 0.5-20 ng/ml of plasma and utilized in the analysis of plasma samples from human clinical studies. The analytical methodology has also been adapted for analysis of compound 1 in human skin blister fluid after topical administration of 1.
authors.affiliation: Merck Sharp & Dohme Research Laboratories, West Point, PA 19486.
authors.forename: B K
authors.initials: BK
authors.lastname: Matuszewski
authors.forename: M L
authors.initials: ML
authors.lastname: Constanzer
authors.forename: W F
authors.initials: WF
authors.lastname: Bayne
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood* / Q000493:pharmacokinetics; D001572:Benzofurans / Q000097:blood* / Q000493:pharmacokinetics; D001768:Blister / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004563:Electrochemistry; D006801:Humans; D011565:Psoriasis / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000893:Anti-Inflammatory Agents; D001572:Benzofurans; C053727:L 651896