Culture of bovine mammary epithelial cells in D-valine modified medium: selective removal of contaminating fibroblasts.
Bovine mammary epithelial cells were cultured in nutrient medium containing D-valine substituted for L-valine in an attempt to control fibroblast overgrowth. Contamination of epithelial cell cultures with fibroblasts was prevented in cultures maintained in D-valine but not in control cultures containing L-valine. Microscopic examination of cells cultured in the D-valine medium demonstrated similar morphology as controls after 27 days in culture. Concentration of alpha-lactalbumin in the D-valine medium was similar to levels found in control medium. Results suggest D-valine modified medium controls fibroblast overgrowth of cell cultures, but doesn't impair epithelial cell morphology or function.
Sordillo
L M
LM
Department of Animal Science, University of Tennessee, Knoxville 37901.
Oliver
S P
SP
Akers
R M
RM
D000818:Animals; D002417:Cattle; D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media; D004847:Epithelial Cells; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D007768:Lactalbumin / Q000096:biosynthesis; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D014633:Valine / Q000378:metabolism*
D003470:Culture Media; D007768:Lactalbumin; D014633:Valine