The effect of postfusion cell density on establishment of hybridomas.
The effect of postfusion cell density on establishment of hybridomas in primary microculture was studied. Following fusion of BALB/c spleen and NS0 myeloma cells in the ratio of 5:1 we demonstrated that seeding the cells at a concentration of about 20-25 X 10(4) spleen cells per well resulted in maximal number of wells containing only one growing hybridoma clone (40.1-43.7% plated wells). Importance of generation of microculture with one clone for the effectiveness of preparation of mAb-secreting hybridomas is discussed.
Kontseková
E
E
Department of Microbiology, Virology and Immunology, Comenius University, Bratislava.
Novák
M
M
Kontsek
P
P
Borecký
L
L
Lesso
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002452:Cell Count; D002459:Cell Fusion; D002478:Cells, Cultured; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal