title: Inorganic phosphate and energy charge compartmentation in Ehrlich ascites tumour cells in the presence of glucose and/or glutamine.
abstract: Inorganic phosphate concentrations and the values of energy charge were determined in the cytosolic and mitochondrial compartments of Ehrlich ascites tumour cells incubated with 5 mM glucose and/or 0.5 mM glutamine. The initial value of inorganic phosphate concentration in the cytosolic compartment decreased in the three incubation conditions assayed; this decrease was greater when glucose was present in the incubation medium. Mitochondrial inorganic phosphate concentration diminished in incubations with only glucose and raised in incubations with only glutamine.
authors.affiliation: Departamento de Bioquímica, Biología Molecular y Química Orgánica, Facultad de Ciencias, Universidad de Málaga, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Medina
authors.forename: A R
authors.initials: AR
authors.lastname: Quesada
authors.forename: F J
authors.initials: FJ
authors.lastname: Márquez
authors.forename: F
authors.initials: F
authors.lastname: Sánchez-Jiménez
authors.forename: I
authors.initials: I
authors.lastname: Núñez de Castro
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D005973:Glutamine / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*
chemical_list: D010710:Phosphates; D005973:Glutamine; D005947:Glucose