Measuring human IgG4 by a two-site immunoradiometric assay with monoclonal antibodies.
By using 2 monoclonal antibodies, we developed a solid-phase 2-site immunoradiometric assay for measuring human IgG4. The measuring range (0.05-20 micrograms/ml) covered more than 2 orders of magnitude. The sensitivity level should make this assay especially useful when IgG4 concentrations are too low to be detected by conventional methods.
Bui
R H
RH
Department of Pediatrics, Loma Linda University, Calif.
Molinaro
G A
GA
Eby
W C
WC
Jauhar
R
R
Mace
J W
JW
D000911:Antibodies, Monoclonal; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007223:Infant; D007231:Infant, Newborn; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes