title: Ribonuclease A--a complete proton NMR fingerprint using TOCSY and NOESY experiments in water.
abstract: In our attempts to obtain total sequential assignment of the 1H NMR spectrum of ribonuclease A, several published water suppression techniques were tested and assessed. The jump- and-return sequence and its echo hybrid were used with considerable success in both the TOCSY and NOESY experiments on 3mM ribonuclease A solutions. The NMR approach used here may be of general applicability for 1H NMR studies of proteins in water of concentrations under 5mM.
authors.affiliation: Biological NMR Centre, University of Leicester, United Kingdom.
authors.forename: L Y
authors.initials: LY
authors.lastname: Lian
mesh_terms: D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011487:Protein Conformation; D012259:Ribonuclease, Pancreatic; D014867:Water
chemical_list: D014867:Water; D006859:Hydrogen; D012259:Ribonuclease, Pancreatic