Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing.
We describe an investigation of proteinuria using Pharmacia PhastSystemTM electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.
Jackson
P J
PJ
Unit for Cancer Research, University of Leeds, UK.
Sampson
C J
CJ
Cooper
E H
EH
Heney
D
D
Brocklebank
J T
JT
D001322:Autoanalysis; D001536:Bence Jones Protein / Q000652:urine; D002648:Child; D003928:Diabetic Nephropathies / Q000652:urine; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008180:Lupus Erythematosus, Systemic / Q000652:urine; D008297:Male; D009135:Muscular Diseases / Q000652:urine; D011507:Proteinuria / Q000652:urine*; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate; D001536:Bence Jones Protein