In vitro interactions of amikacin and beta-lactam antibiotics against amikacin-resistant gram-negative bacilli.
We tested 42 strains of amikacin-resistant gram-negative bacilli with amikacin in combination with six beta-lactam antibiotics using the checkerboard and time-kill curve techniques. Synergism was demonstrated with time-killing curve in 43-68% of the strains tested. Ceftazidime plus amikacin was the most active combination by the checkerboard technique, while amikacin-cefoperazone was the most active combination by the time-killing curve technique against Pseudomonas aeruginosa. Discrepancies were found between the results of the two methods used.
Alvarez
S
S
Infectious Disease Section, VA Medical Center, Johnson City, Tenn.
Jones
M
M
Holtsclaw-Berk
S
S
Berk
S L
SL
D000583:Amikacin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004352:Drug Resistance, Microbial; D004357:Drug Synergism; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D000583:Amikacin