title: Induction of an antibody that catalyzes the hydrolysis of an amide bond.
abstract: Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.
authors.affiliation: Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
authors.forename: K D
authors.initials: KD
authors.lastname: Janda
authors.forename: D
authors.initials: D
authors.lastname: Schloeder
authors.forename: S J
authors.initials: SJ
authors.lastname: Benkovic
authors.forename: R A
authors.initials: RA
authors.lastname: Lerner
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000502:physiology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D006433:Hemocyanins / Q000031:analogs & derivatives / Q000276:immunology; D006868:Hydrolysis; D007114:Immunization; D007700:Kinetics; D051379:Mice; D009943:Organophosphorus Compounds / Q000276:immunology; D013379:Substrate Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D009943:Organophosphorus Compounds; C056997:keyhole limpet hemocyanin phosphonamidate conjugate; D006433:Hemocyanins; D000581:Amidohydrolases; C032808:keyhole-limpet hemocyanin