Isolation and sequence of rat peptide YY and neuropeptide Y.
Rat peptide YY and rat neuropeptide Y have been isolated in parallel from colon and brain extracts respectively, using salt fractionation, gel filtration chromatography, cation-exchange HPLC, and reverse phase phenyl-silica HPLC. Immunoreactivity was identified using a combination of 3 NPY immunoassays which exhibit differing cross-reactivities for PYY (90%, less than 0.01% and 30% respectively). The yield at the final purification step was 1.2 nmol rPYY and 0.5 nmol rNPY. Half of each purified peptide was subjected to complete microsequence analysis. This showed that while rat NPY was structurally identical to human NPY, the sequence of PYY from rat colon was the same as porcine PYY isolated from extracts of duodenum.
Corder
R
R
Department of Medicine, University Hospital, Geneva, Switzerland.
Gaillard
R C
RC
Böhlen
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005768:Gastrointestinal Hormones / Q000302:isolation & purification*; D007118:Immunoassay / Q000379:methods; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000302:isolation & purification*; D019894:Peptide YY; D010455:Peptides / Q000302:isolation & purification*; D011874:Radiometry; D051381:Rats
D005768:Gastrointestinal Hormones; D009478:Neuropeptide Y; D010455:Peptides; D019894:Peptide YY