title: Effect of osmolarity on potassium transport in isolated cerebral microvessels.
abstract: Potassium transport in microvessels isolated from rat brain by a technique involving density gradient centrifugation was studied in HEPES buffer solutions of varying osmolarity from 200 to 420 mosmols, containing different concentration of sodium chloride, choline chloride, or sodium nitrate. The flux of 86Rb (as a tracer for K) into and out of the endothelial cells was estimated. Potassium influx was very sensitive to the osmolarity of the medium. Ouabain-insensitive K-component was reduced in hypotonic medium and was increased in medium made hypertonic with sodium chloride or mannitol. Choline chloride replacement caused a large reduction in K influx. Potassium influx was significant decrease when nitrate is substituted for chloride ion in isotonic and hypertonic media, whereas a slight decrease was found in hypotonic medium. The decrease of K influx in the ion-replacement medium is due to a decrement of the ouabain-insensitive component. Potassium efflux was unchanged in hypotonic medium but was somewhat reduced in hypertonic medium. The marked effect of medium osmolarity on K fluxes suggests that these fluxes may be responsible for the volume regulatory K movements. The possible mechanism of changes of K flux under anisotonic media is also discussed.
authors.affiliation: Department of Biology, National Cheng Kung University, Tainan, Taiwan, R.O.C.
authors.forename: J D
authors.initials: JD
authors.lastname: Lin
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000098:blood supply* / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D008297:Male; D008833:Microcirculation / Q000378:metabolism; D009994:Osmolar Concentration; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D003470:Culture Media; D012965:Sodium Chloride; D010042:Ouabain; D011188:Potassium