title: Tissue-specific regulation of guinea pig lipoprotein lipase; effects of nutritional state and of tumor necrosis factor on mRNA levels in adipose tissue, heart and liver.
abstract: Levels of mRNA for lipoprotein lipase (LPL) in guinea pig epididymal adipose tissue, heart and liver were determined by dot blot analysis of total RNA using a cDNA probe complementary to the coding region, and compared to the LPL activity. For adipose tissue we also measured the incorporation of radioactivity into immunoprecipitable LPL after pulse-labeling with [35S]methionine. LPL activity was 93%, LPL mRNA 82% and LPL synthesis 85% lower in epididymal fat pads from animals fasted for 48 h compared to rigorously fed animals. In contrast, neither LPL activity nor LPL mRNA levels differed in heart. A single dose of tumor necrosis factor (TNF) decreased LPL activity and LPL mRNA in fat pads with no effects in heart. In the liver, TNF caused a marked increase in LPL mRNA levels, which are normally very low. Northern-blot analysis confirmed a previous observation that the patterns of mRNA species differ between heart, in which a 3.8-kb mRNA dominates, and adipose tissue, in which the LPL mRNAs of 3.3 and 2.1 kb occur in similar abundance as the 3.8-kb species.
authors.affiliation: Department of Medical Biochemistry, University of Gothenburg, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Enerbäck
authors.forename: H
authors.initials: H
authors.lastname: Semb
authors.forename: J
authors.initials: J
authors.lastname: Tavernier
authors.forename: G
authors.initials: G
authors.lastname: Bjursell
authors.forename: T
authors.initials: T
authors.lastname: Olivecrona
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D005786:Gene Expression Regulation; D005796:Genes / Q000187:drug effects*; D006168:Guinea Pigs; D008071:Lipoprotein Lipase / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D009747:Nutritional Physiological Phenomena; D009928:Organ Specificity; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology*; D012016:Reference Values; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D008071:Lipoprotein Lipase