title: The mechanism of experimental adipocere formation: hydration and dehydrogenation in microbial synthesis of hydroxy and oxo fatty acids.
abstract: The enzyme preparations from Flavobacterium meningosepticum solubilized by sonication catalyzed not only hydration of oleic acid to produce 10-hydroxystearic acid but dehydrogenation of this product. The mechanism of the hydration and dehydrogenation was proved by gas chromatography-mass spectrometry of 10-hydroxy and 10-oxostearic acids produced in the presence of D2O or H2(18)O. The activity of these enzymes was increased by preincubating Flavobacterium meningosepticum with oleic acid.
authors.affiliation: Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Gotouda
authors.forename: T
authors.initials: T
authors.lastname: Takatori
authors.forename: K
authors.initials: K
authors.lastname: Terazawa
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: H
authors.initials: H
authors.lastname: Tarao
mesh_terms: D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000378:metabolism*; D005417:Flavobacterium / Q000201:enzymology / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006880:Hydroxy Acids / Q000378:metabolism*; D007651:Keto Acids / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D011180:Postmortem Changes
chemical_list: D005227:Fatty Acids; D006880:Hydroxy Acids; D007651:Keto Acids; D009829:Oleic Acids; D019301:Oleic Acid