[Immunochemical detection and characteristics of the subunit composition of phenylalanine hydroxylase in the brain of man].
Immunochemical properties and subunit structure of an antigen were characterized in autopsy specimens of human liver and brain, using antiserum against human phenylalanine hydroxylase. An identical antigen was revealed in extracts of organs by immunoelectrophoresis. Its content was 1.5-2.0 mg/g tissue in the liver and 20-40 micrograms/g tissue in the brain. One L enzyme subunit and two H subunits were identified in the liver extracts after two-dimensional electrophoresis followed by immunoblotting. Subunit structure of phenylalanine hydroxylase in the brain was similar to that in the liver. The molecular weight of L subunit was 55,000 and it was located in the same area as albumin isoforms. The molecular weight of H subunits was 57,000 and they differed from L subunits in pI. The antigen was purified from crude extracts of biopsy liver by affinity chromatography on immunoadsorbent to phenylalanine hydroxylase and showed phenylalanine hydroxylase activity. An antigen with similar molecular weight was also purified from the brain extract by the same method. These data suggest that phenylalanine hydroxylase can be present in the human brain.
Chestkov
V V
VV
Laptev
A V
AV
D001921:Brain / Q000201:enzymology*; D006801:Humans; D008099:Liver / Q000201:enzymology; D010651:Phenylalanine Hydroxylase / Q000032:analysis*
D010651:Phenylalanine Hydroxylase
Immunokhimicheskoe vyiavlenie i kharakteristika sub"edinichnogo sostava fenilalaningidroksilazy v mozge cheloveka.