Effect of glucocorticoid hormones on the growth of human fibroblast cells and interferon production.
Human diploid fibroblasts could be passaged serially on microcarriers and used as an efficient system for the bulk production of human interferon-beta (IFN-beta), provided that appropriate amounts of glucocorticoid hormones were added to the fetal bovine serum used in the medium; alternatively, precolostrum newborn calf serum, which has a relatively high content of hydrocortisone, could be used.
Sano
E
E
Basic Research Laboratories, Toray Industries Inc., Kamakura, Japan.
Iizuka
M
M
Kobayashi
S
S
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D004171:Diploidy; D005347:Fibroblasts / Q000166:cytology*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*
D003470:Culture Media; D005938:Glucocorticoids; D007370:Interferon Type I