[Inhibition with tetracaine of passive Ca2+ flux through the membrane of the sarcoplasmic reticulum of skeletal muscles].
The initial fast phase of Ca2+ efflux from vesicles of sarcoplasmic reticulum (SR) loaded with Ca2+ passively or in ATP-dependent process and the initial fast phase of Ca2+ influx into the SR vesicles are inhibited by 0.5 mM tetracaine. The inhibitory action of tetracaine observed both with the heavy and light SR fractions is competitive with Ca2+, decreased with varying pH from 5.8 to 7.8 and temperature from 20 degrees C to 37 degrees C. Choline chloride in the concentration of 150 mM at 37 degrees C prevents the inhibitory action of tetracaine. It is supposed that electrostatic interaction plays an essential role in tetracaine-inhibition of passive Ca2+ fluxes through the membrane.
Diadiusha
G P
GP
Tugaĭ
V A
VA
Zemlianaia
N N
NN
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D013748:Tetracaine / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D013748:Tetracaine; D002118:Calcium
Ingibirovanie tetrakainom passivnykh potokov Ca2+ cherez membranu sarkoplazmaticheskogo retikuluma skeletnykh myshts.