Presence of epoxide hydrolase and glutathione S-transferase in human pulmonary alveolar macrophages.
Pulmonary alveolar macrophages (PAMs) were obtained from 11 patients by bronchoalveolar lavage. Epoxide hydrolase and glutathione S-transferase in sonicated PAMs were measured using benzo(a)pyrene-4,5-oxide as the substrate. The activity of epoxide hydrolase was 0.24 +/- 0.10 nmol/min/mg protein (mean +/- SD), and of glutathione S-transferase 0.22 +/- 0.12 nmol/min/mg protein. There was a significant difference in enzyme activities in the PAMs from smokers and non-smokers. Epoxides may be metabolized in PAMs.
Petruzzelli
S
S
Institute of 2nd Medical Clinic, University of Pisa, Italy.
Bernard
P
P
Paoletti
P
P
Rane
A
A
Giuntini
C
C
Pacifici
G M
GM
D004851:Epoxide Hydrolases / Q000378:metabolism*; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D011650:Pulmonary Alveoli / Q000166:cytology / Q000201:enzymology*
D005982:Glutathione Transferase; D004851:Epoxide Hydrolases