A ligase-mediated gene detection technique.
An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
Landegren
U
U
Division of Biology, California Institute of Technology, Pasadena 91125.
Kaiser
R
R
Sanders
J
J
Hood
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D011088:DNA Ligases / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism; D005456:Fluorescent Dyes; D005914:Globins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D011118:Polynucleotide Ligases / Q000378:metabolism*
D004274:DNA, Recombinant; D005456:Fluorescent Dyes; D005914:Globins; D004247:DNA; D011088:DNA Ligases; D011118:Polynucleotide Ligases