title: Plasma haemoglobin determination using chlorpromazine as a non-carcinogenic reagent.
abstract: Plasma haemoglobin was assayed with the non-carcinogenic reagent phenothiazine. This method is sensitive and allows the measurement of plasma haemoglobin concentrations in the range 4-500 mg/l with a within-run CV of 2.1%, and a between-run CV of 4.3%. A spectrophotometric scanning method (x) based on the determination of haemoglobin as haemiglobin cyanide using the Soret band at 419 nm correlated well with the phenothiazine method (y): y = 1.07x + 15.8, r = 0.995, n = 31. It was found that the absorbances in the phenothiazine method were markedly dependent on the concentration of phosphoric acid.
authors.affiliation: Department of Clinical Chemistry, Kuopio University Central Hospital, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Nyyssönen
authors.forename: M T
authors.initials: MT
authors.lastname: Parviainen
authors.forename: I M
authors.initials: IM
authors.lastname: Penttilä
mesh_terms: D002746:Chlorpromazine; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D013053:Spectrophotometry
chemical_list: D006454:Hemoglobins; D007202:Indicators and Reagents; D002746:Chlorpromazine