Effect of elastin peptides and N-formyl-methionyl-leucyl phenylalanine on cytosolic free calcium in polymorphonuclear leukocytes of healthy middle-aged and elderly subjects.
The effect of elastin peptides (kappa-elastin, KE) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) on cytosolic free calcium in polymorphonuclear leukocytes (PMNLs) of healthy middle-aged (35-45 years) and elderly (greater than 60 years) patients with normal and high serum cholesterol level was investigated. The cytosolic free calcium [( Ca2+]i) elevation after stimulation with these compounds was decreased in PMNLs of the aged groups compared to the healthy middle-aged group. The guanine nucleotide binding regulatory Gi protein inhibitor, pertussis toxin (PT) prevented the enhancing effect of KE and FMLP on PMNL free calcium of healthy middle-aged subjects, but could not completely abolish the [Ca2+]i elevation in PMNLs of aged subjects.
Varga
Z
Z
First Department of Medicine, University Medical School, Debrecen, Hungary.
Kovács
E M
EM
Paragh
G
G
Jacob
M P
MP
Robert
L
L
Fülöp
T
T
D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004549:Elastin / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*
D010455:Peptides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D004549:Elastin; D002118:Calcium