Effect of ethanol and linoleic acid on changes in biliary excretion of iodothyronines possibly related to thyroxine deiodination in rat liver.
Groups of male rats weighing about 350 g were inserted polyethylene tubings into bile duct and femoral vein under pentobarbital anesthesia. Several iodothyronines (i.e. T4, T3, rT3, 3,5-T2, 3,3'-T2 and 3',5'-T2) were estimated in 2-hr portions of bile with the aid of specific radioimmunoassay. After the infusion of ethanol (0.3 ml/hr/rat for 4 hr) an increase of biliary excretion of rT3 and a decrease of 3,5-T2 was found as compared to controls. When 5 mg linoleic acid was added to 1.2 ml ethanol, the increase of rT3 was significantly higher than that after ethanol only and, in addition, significant increase of 3',5'-T2 excretion was found. It was concluded that both ethanol and unsaturated fatty acids may inhibit 5'-monodeiodination in the liver and that unsaturated nonesterified fatty acids may exert such effect even when administered intravenously without underlying metabolic disorders.
Langer
P
P
Institute of Experimental Endocrinology, Center of Physiological Sciences SAV, Bratislava, Czechoslovakia.
Földes
O
O
Gschendtová
K
K
D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism*; D004104:Diiodothyronines / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D008041:Linoleic Acids / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013974:Thyroxine / Q000378:metabolism*; D014284:Triiodothyronine / Q000378:metabolism*
D004104:Diiodothyronines; D008041:Linoleic Acids; D014284:Triiodothyronine; D000431:Ethanol; C030103:3,5-diiodothyronine; D013974:Thyroxine