[Isolation of modified allergens from bee venom].
To obtain modified bee venom (BV) allergens, covalent binding of BV with previously carboxylated polyethylene glycol (PEG) has been used. The conjugation of BV and PEG has been achieved by means of carbodiimide. Thus 4 kinds of the conjugate with BV/PEG ratio ranging from 30:1 to 63:1 have been obtained. The study has shown that chemical treatment in the process of this reaction, dialysis and chromatography does not lead to a decrease in the specific activity of BV, while lyophilization produces such an effect. The above method for the modification of BV allergen, used with a view to obtaining high molecular compositions, is reproducible, ensures sufficient yield (about 30%), and permits obtaining conjugates with specified BV/PEG ratios.
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D000485:Allergens / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D001514:Bee Venoms / Q000276:immunology*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000031:analogs & derivatives / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests; D008722:Methods; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000485:Allergens; D001514:Bee Venoms; D003432:Cross-Linking Reagents; D011092:Polyethylene Glycols; C041829:1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide methiodide; D005022:Ethyldimethylaminopropyl Carbodiimide
Poluchenie modifitsirovannykh allergenov pchelinogo iada.