title: Detection of two heme oxygenase isoforms in the human testis.
abstract: This study shows heme oxygenase multiplicity is common to rat and human tissues. The isozymes in man and rat, however, are heterogenous proteins that share certain characteristics. Two forms of heme oxygenase, HO-1 and HO-2, were identified in human testis. HO-2 form was the prevalent form. Human and rat HO-1 differed in chromatographic behavior and molecular weight; human HO-1 was a larger molecule (35,400 vs 30,000). The two forms, however, were similar in that immunochemically human HO-1 exhibited reactivity toward antibody to rat HO-1. Human and rat HO-2 also were dissimilar in chromatographic behavior and showed only a weak immunological cross-reactivity. Human and rat HO-1 were essentially the same size. As in rat organs, the microsomal cytochrome P-450 content in human testis was reciprocal to heme oxygenase activity.
authors.affiliation: Department of Biophysics, University of Rochester Medical Center, New York 14642.
authors.forename: G M
authors.initials: GM
authors.lastname: Trakshel
authors.forename: M D
authors.initials: MD
authors.lastname: Maines
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D006899:Mixed Function Oxygenases / Q000302:isolation & purification*; D008970:Molecular Weight; D009928:Organ Specificity; D051381:Rats; D013045:Species Specificity; D013737:Testis / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D006419:Heme Oxygenase (Decyclizing)