[Experimental and clinical study on flow cytometric DNA analysis of human breast carcinoma].
Flow cytometric DNA analysis was performed on four human breast carcinoma xenografts serially transplanted into nude mice (MX-1, T-61, R-27 and MCF-7) together with 31 surgical specimens of breast carcinoma. Mechanically dissociated tumor cells were stained with propidium iodide and histograms were obtained by counting at least 2 x 10(4) cells using EPICS-V flow cytometer. Tumor ploidy was expressed as DNA Index using internal standard of chicken red blood cells and the percentage of cells in the S phase of cell cycle was determined by Bagwell's program. All of four xenografts and 23 of 31 clinical specimens showed non-diploid pattern. Statistically significant correlation was observed between %S and tumor doubling time of human tumor xenografts, viewed rapid growing tumor revealed high %S. In clinical cases, statistically significant correlation was present between %S and histological grade and the state of hormone receptors. Histological grade III tumors had a significantly higher %S than that of histological grade I tumors. ER negative tumors showed a significantly higher mean %S than that of ER positive tumors. Similarly, PgR negative tumors possessed a significantly higher mean %S than that of PgR positive tumors. However, no significant correlation was found between ploidy pattern and clinicopathological parameters. It was concluded that flow cytometric %S might be useful to estimate the biological malignancy of human breast carcinomas.
Nakamura
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Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
D000328:Adult; D000368:Aged; D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011003:Ploidies; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis
D004273:DNA, Neoplasm; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone