Different response of chicken embryo fibroblasts and hepatocytes to the interference of certain antioxidants on the binding of [G-3H]benzo[a]pyrene to DNA.
The effects of treatment with reduced glutathione, cysteine and ascorbic acid on chick embryo fibroblasts and hepatocytes cultured in vitro in the presence of [G-3H]benzo[a]pyrene ([3H]BP) were studied in an attempt to evaluate the capacity of these antioxidants to interfere with the binding of the carcinogen to DNA in these two cell populations. The results revealed that in the absence of treatment with antioxidants, the carcinogen bound to the DNA of the hepatocytes less than it did to that of the fibroblasts, despite the fact that the two cell populations manifest more or less the same uptake of [G-3H]benzo[a]pyrene from the medium. Moreover the hepatocytes, unlike the fibroblasts, seemed to reap little advantage from the known capacity of the antioxidants to interfere with the binding of the carcinogen to DNA.
Liotti
F S
FS
Institute of General Biology, University of Perugia, Italy.
Pelliccia
C
C
Pezzetti
F
F
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D003545:Cysteine / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D019803:Glutathione Disulfide; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D014316:Tritium
D000975:Antioxidants; D014316:Tritium; D001564:Benzo(a)pyrene; D004247:DNA; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid; D019803:Glutathione Disulfide