[A study of DNA ploidy patterns of gastric cancers].
The nuclear DNA fluorescence of gastric cancer cells which were obtained from endoscopic biopsies and paraffin block specimen was measured. Histograms in 142 cases could be roughly classified into four types: Type Ia, means diploid pattern without polyploid cells, type Ib, diploid pattern with polyploid cells, type II, heteroploid pattern and type III, mosaic pattern. Although these DNA patterns showed no correlation with histological types, type Ia was frequently found in early-stage cancer and type III in advanced-stage cancer. In cases without vascular invasion and in each metastasis, type Ia were significantly numerous. With regard to prognosis, the survival rate was becoming poorer in order of Ia less than Ib less than II less than III. In 41 cases out of 45 advanced-stage cancers, DNA patterns of superficial and deep portion of gastric cancer were found the same. In 5 cases, DNA patterns of cancer cells in metastatic lymph nodes were coincident with DNA patterns in gastric cancers. These results suggested that the measurement of nuclear DNA pattern may provides a biological activity of gastric cancer cells and preoperative examination of endoscopic biopsy specimens from this aspect is also clinically important.
Yoshino
H
H
First Department of Surgery, Osaka City University of Medicine, Japan.
D000230:Adenocarcinoma / Q000032:analysis / Q000473:pathology*; D002467:Cell Nucleus / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D008207:Lymphatic Metastasis; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011003:Ploidies; D011379:Prognosis; D013274:Stomach Neoplasms / Q000032:analysis / Q000473:pathology*
D004273:DNA, Neoplasm