[Purification and properties of a protein from the nuclear fraction of HeLa cells binding to a cloned fragment of human satellite DNA III].
In nuclear extract of HeLa cells two proteins were identified having the specific binding activity to cloned 1.8kb fragment of human satellite DNA III (HS3). One of the satellite binding proteins (SBP1) purified by column chromatography using DEAE-, phospho- and DNA-cellulose steps interacted also with adenovirus 5 replication enhancer (ARE), another protein (SBP2) was separated during phosphocellulose chromatography from ARE-binding protein. It is suggested that SBP1 is possibly identical to the nuclear factor I purified earlier from the nuclear extract of HeLa cells by other authors.
Svetlova
M P
MP
Perelygina
L M
LM
Dube
Sh
Sh
Tomilin
N V
NV
D002848:Chromatography, DEAE-Cellulose; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans
D004276:DNA, Satellite; D004268:DNA-Binding Proteins
Ochistka iz iadernoĭ fraktsii kletok HeLa i nekotorye svoĭstva belka, sviazyvaiushchego klonirovannyĭ fragment satellitnoĭ DNK III cheloveka.