title: Electrophysiology of cell volume regulation in proximal tubules of the mouse kidney.
abstract: The present study has been designed to test for the influence of cell swelling on the potential difference and conductive properties of the basolateral cell membrane in isolated perfused proximal tubules. During control conditions the potential difference across the basolateral cell membrane (PDbl) is -65 +/- 1 mV (n = 74). Decrease of peritubular osmolarity by 80 mosmol/l depolarizes the basolateral cell membrane by +7.8 +/- 0.5 mV (n = 42). An increase of bath potassium concentration from 5 to 20 mmol/l depolarizes the basolateral cell membrane by +25 +/- 1 mV (n = 11), an increase of bath bicarbonate concentration from 20 to 60 mmol/l hyperpolarizes the basolateral cell membrane by -3.2 +/- 0.5 mV (n = 13). A decrease of bath chloride concentration from 79.6 to 27 mmol/l hyperpolarizes the basolateral cell membrane by -1.8 +/- 0.7 mV (n = 6). During reduced bath osmolarity, the influence of altered bath potassium concentration on PDbl is decreased (delta PDbl = +16 +/- 2 mV, n = 11), the influence of altered bicarbonate concentration on PDbl is increased (delta PDbl = -6.0 +/- 0.8 mV, n = 13), and the influence of altered bath chloride concentration on PDbl is unaffected (delta PDbl = -1.8 +/- 0.6 mV, n = 6). Barium depolarizes the basolateral cell membrane to -28 +/- 2 mV (n = 16). In the presence of 1 mmol/l barium, decrease of peritubular osmolarity by 80 mosmol/l leads to a transient hyperpolarization of the basolateral cell membrane by -5.9 +/- 0.5 mV (n = 16).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institut für Physiologie der Universität Innsbruck, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Völkl
authors.forename: F
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000502:physiology; D002712:Chlorides / Q000502:physiology; D007038:Hypotonic Solutions; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D011188:Potassium / Q000502:physiology
chemical_list: D001639:Bicarbonates; D002712:Chlorides; D007038:Hypotonic Solutions; D011188:Potassium