Red blood cell membrane microviscosity correlates with posttransfusion survival.
The cholesterol/phospholipid and sphingomyelin/phospholipid ratios of red blood cells stored for 42 days were unchanged after storage. However, the total phospholipid concentration in the red cells decreased suggesting a loss of red cell membrane during storage. The 24 h survival of the stored red cells was assessed by the Cr technique in homologous donors. A decrease in fluorescence polarization of diphenylhexatriene incorporated into the membrane was observed following storage which correlated with survival. Thus, molecular defects in the lipid bilayer are associated with long-term storage of red blood cells.
McLean
L R
LR
Merrell Dow Research Institute, Cincinnati, Ohio 45215.
Grote
C
C
Silberstein
E B
EB
McGill
M
M
D001793:Blood Preservation; D001803:Blood Transfusion; D002470:Cell Survival; D002784:Cholesterol / Q000097:blood*; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000032:analysis / Q000648:ultrastructure*; D004912:Erythrocytes / Q000166:cytology*; D006461:Hemolysis; D006801:Humans; D008563:Membrane Lipids / Q000097:blood*; D010743:Phospholipids / Q000097:blood*; D013109:Sphingomyelins / Q000097:blood; D014783:Viscosity
D008563:Membrane Lipids; D010743:Phospholipids; D013109:Sphingomyelins; D002784:Cholesterol