The use of iodinated density gradient media for the isolation of rod outer segments.
Sucrose, nycodenz, metrizamide and a mixture of equal volumes of sucrose and metrizamide were used as density gradient media for the isolation of retinal rod outer segments. The high osmolarity of sucrose had a strongly negative effect on the nature of the rod outer segments, whereas they were much better preserved using iodinated density gradient media such as nycodenz and metrizamide for their isolation.
Delgadillo
R A
RA
Institute of Experimental Ophthalmology, University of Antwerp, Belgium.
Smets
R M
RM
Yang
Q R
QR
Vanden Berghe
D
D
Neetens
A
A
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D007455:Iodine; D008854:Microscopy, Electron; D010786:Photoreceptor Cells; D012374:Rod Cell Outer Segment
D007455:Iodine