Mass spectrometric identification of urinary and plasma metabolites of 9-hydroxy-19,20-bis-nor-prostanoic acid (rosaprostol).
9-Hydroxy-19,20-bis-nor-prostanoic acid (Rosaprostol) is an antiulcer compound with antisecretory and cytoprotective action. We studied the metabolites of Rosaprostol found in human plasma and in human and rat urine. Sixteen different metabolites were tentatively identified on the basis of their mass spectra. Two presumed metabolites were synthesized. To clarify the identities of some of them, deuterated Rosaprostol was administered to rats and mass spectra of the deuterated and protonated metabolites were examined. Rosaprostol is metabolized following three metabolic pathways leading, combined, to oxidized compounds with a lower number of carbons than the parent drug.
Benfenati
E
E
Laboratory of Environmental Pharmacology and Toxicology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Fanelli
R
R
Chiabrando
C
C
Valcavi
U
U
Bosone
E
E
Farina
P
P
D000818:Animals; D000897:Anti-Ulcer Agents / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D011465:Prostaglandins, Synthetic / Q000378:metabolism*; D011466:Prostanoic Acids / Q000378:metabolism*; D051381:Rats
D000897:Anti-Ulcer Agents; D005227:Fatty Acids; D011465:Prostaglandins, Synthetic; D011466:Prostanoic Acids; C035288:rosaprostol