A diffusion limited reaction theory for a microtiter plate assay.
Calculations are presented describing the diffusion limited kinetics of a solid-phase immunoassay in which reactants are immobilized at the inner surface of a cylindrical well. The calculations refer to an unstirred situation and simplified expressions are presented which can be used for calibration and optimization of the assay.
Stenberg
M
M
Department of Solid State Electronics, Chalmers University of Technology, Göteborg, Sweden.
Werthén
M
M
Theander
S
S
Nygren
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002138:Calibration; D004058:Diffusion; D004140:Dinitrophenols / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000592:standards; D004800:Enzymes, Immobilized; D007700:Kinetics; D051379:Mice; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D012825:Silicon; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D004140:Dinitrophenols; D004800:Enzymes, Immobilized; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D012825:Silicon