Multiple nuclear factors interact with promoter sequences of the urokinase-type plasminogen activator gene.
To characterize proteins that bind to the cyclic AMP inducible promoter of the urokinase-type plasminogen activator gene, we performed a DNAase I footprinting analysis. Within 500 nucleotides upstream of the transcription start site we found eight protected regions due to at least four different binding proteins. Among these is a single binding site for the transcription factor CTF/NF1, which is flanked on each side by two conserved binding sites for the transcription factor Sp1. A region at -380, which shares a similarity with sequences observed in the corresponding regions of other cyclic AMP regulated genes, was protected. This binding site contains a sequence of ten nucleotides which is repeated further upstream at -480 and also protected against DNAase I digestion. Comparisons of extracts from four different cell lines revealed that all DNA binding factors are present in nuclei of uPA expressing and nonexpressing cells. Mechanism underlying hormonal regulation of the gene is discussed.
von der Ahe
D
D
Friedrich Miescher-Institut, Basel, Switzerland.
Pearson
D
D
Nakagawa
J
J
Rajput
B
B
Nagamine
Y
Y
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000502:physiology; D003850:Deoxyribonuclease I; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013552:Swine; D014157:Transcription Factors / Q000502:physiology*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D003850:Deoxyribonuclease I; D014568:Urokinase-Type Plasminogen Activator