Identification of dekamycin antibiotics.
Dekamycin was isolated from strain DK5 of Streptomyces fradiae, and purified by ion-exchange column chromatography. Excellent separation of N-acetylated derivatives of the antibiotic mixture was achieved by two different methods, flash chromatography and high-performance liquid chromatography. Mass spectrometry (252Cf plasma desorption mass spectrometry, fast atom bombardment) for the analysis of N-acetyldekamycins (neomycin B, neomycin C, neamine and ribostamycin) was mainly used to confirm the molecular weight of a structure deduced by 1H and 13C NMR analyses.
Van
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TD
Département de Chimie, Ecole Polytechnique, Palaiseau, France.
Khac
D D
DD
Fetizon
M
M
Tabet
J C
JC
Le Beyec
Y
Y
Quyen
T C
TC
D000107:Acetylation; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000096:biosynthesis; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008970:Molecular Weight; D013302:Streptomyces / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C056832:dekamycin