Intracellular development of choleraphage phi 149 under permissive and nonpermissive conditions: an electron microscopic study.
Intracellular development of choleraphage phi 149 following infection of Vibrio cholerae and Vibrio eltor cells under different conditions was examined by thin-section electron microscopy. Degradation of the host DNA following infection and formation of mature phage particles inside the infected cells were demonstrated. The concatemeric DNA intermediate formed during intracellular replication of phi 149 DNA in permissive hosts was resolved. In confirmation of biochemical evidence, no concatemeric DNA intermediate was observed for infection in high-phosphate medium.
Majumdar
S
S
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
Dey
S N
SN
Chowdhury
R
R
Dutta
C
C
Das
J
J
D001435:Bacteriophages / Q000254:growth & development* / Q000648:ultrastructure; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D008242:Lysogeny; D008854:Microscopy, Electron; D010710:Phosphates / Q000494:pharmacology; D014733:Vibrio / Q000648:ultrastructure; D014734:Vibrio cholerae / Q000648:ultrastructure*
D003470:Culture Media; D004269:DNA, Bacterial; D004279:DNA, Viral; D010710:Phosphates