Enhancement of human neutrophil function by platelets: effects of indomethacin.
To characterize the influence of presence of platelets on human neutrophil function, neutrophil oxidative burst, chemotaxis, leukotriene B4 and prostacyclin generation were examined in the presence of physiologic concentration of platelets (40: 1). Presence of platelets significantly (P less than or equal to 0.05) increased all these neutrophil functions. To determine if cyclooxygenase products are involved in platelet-neutrophil interaction, neutrophils (+/- platelets) were incubated with indomethacin. Although high concentrations of indomethacin (10 microM) inhibited neutrophil (no platelets) chemotaxis and leukotriene B4 generation, these inhibitory effects of indomethacin were attenuated in the presence of platelets. Thus presence of platelets enhances neutrophil activity and overcomes the inhibitory effects of indomethacin on neutrophils.
Dinerman
J
J
Department of Medicine, University of Florida, College of Medicine, Gainesville.
Mehta
J
J
Lawson
D
D
Mehta
P
P
D001792:Blood Platelets / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*
D007213:Indomethacin