Comparison of the amino acid sequences of human protamines HP2 and HP3 and of intermediate basic nuclear proteins HPS1 and HPS2. Structural evidence that HPS1 and HPS2 are pro-protamines.
Two intermediate nuclear basic proteins HPS1 and HPS2 were isolated from human sperm. They were characterized by their electrophoretic mobility in acid-urea gels, their amino acid composition, and their peptide maps after digestion by endoproteinase Lys-C and by endoproteinase Glu-C. Their amino-terminal amino acid sequences have also been determined. The structural data thus obtained suggest that HPS1 and HPS2 are precursors of human protamines HP2 and HP3.
Sautière
P
P
Unité Associée Centre National de la Recherche Scientifique No. 409, Institut de Recherches sur le Cancer, Lille, France.
Martinage
A
A
Bélaiche
D
D
Arkhis
A
A
Chevaillier
P
P
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis*; D011479:Protamines / Q000032:analysis*
D000596:Amino Acids; D009687:Nuclear Proteins; D011479:Protamines; C068621:protamine 2; C048529:protamine P2; C052196:protamine P3