Analysis of glutathione conjugates and related compounds by thermospray mass spectrometry.
A series of 17 cysteine, N-acetyl cysteine, glutathione, and N-trifluoroacetyl glutathione conjugates have been prepared, and their thermospray (TSP) spectra have been recorded in the positive and negative ion modes. The compounds undergo extensive fragmentation, which primarily occurs at the carbon-sulfur bonds. For most of the compounds, positive ion TSP is more sensitive than negative ion thermospray. Probably due to the thermal lability of these adducts, the quality of the spectra obtained are dependent on source conditions, requiring fine control of the vaporization/desolvation process.
Parker
C E
CE
Laboratory of Molecular Biphysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
de Wit
J S
JS
Smith
R W
RW
Gopinathan
M B
MB
Hernandez
O
O
Tomer
K B
KB
Vestal
C H
CH
Sanders
J M
JM
Bend
J R
JR
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione / Q000032:analysis*; D013058:Mass Spectrometry; D008970:Molecular Weight
D005978:Glutathione