title: Sequence and identification of the barley yellow dwarf virus coat protein gene.
abstract: The nucleotide sequence of the coat protein gene of barley yellow dwarf virus (BYDV, PAV serotype) was determined, and the amino acid sequence was deduced. The open reading frame, encoding a protein of relative molecular mass (Mr) 22,047, was confirmed as the coat protein gene by comparison with amino acid sequences of tryptic peptides derived from dissociated virions. In addition, a fragment of this gene expressed in Escherichia coli produced a product which was recognized by antibodies prepared against purified BYDV virions. An overlapping reading frame encoding an Mr 17,147 protein is contained completely within the coat protein gene.
authors.affiliation: CSIRO Division of Plant Industry, Canberra, ACT, Australia.
authors.forename: W A
authors.initials: WA
authors.lastname: Miller
authors.forename: P M
authors.initials: PM
authors.lastname: Waterhouse
authors.forename: A A
authors.initials: AA
authors.lastname: Kortt
authors.forename: W L
authors.initials: WL
authors.lastname: Gerlach
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D004247:DNA