Effect of chronic ethanol ingestion on alpha-mannosidase isoenzymes in rat liver.
Identification of biochemical changes induced by ethanol ingestion would aid in the diagnosis and management of many alcohol-related problems in man. In this paper we identify a pH 5.5 alpha-mannosidase activity in the rat which is affected by chronic ethanol consumption. Chronic (16 wk) ingestion of alcohol (36% of calories) causes the activity of this alpha-mannosidase (thought to be the cytosolic alpha-mannosidase) in liver to decrease by 50%. We hypothesize that this deficiency of (pH 5.5) alpha-mannosidase activity may account for the reduced rate of secretion of glycoproteins by livers of alcohol-fed rats reported by other investigators (Volentine et al, Hepatology 1987;7:490-495).
Lauro
P
P
Department of Microbiology, Biochemistry, and Molecular Biology, School of Medicine, University of Pittsburgh, PA 15261.
Lechner
P S
PS
Okolo
A
A
Eagon
P K
PK
Glew
R H
RH
D000437:Alcoholism / Q000201:enzymology*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D003600:Cytosol / Q000201:enzymology; D006023:Glycoproteins / Q000096:biosynthesis; D006867:Hydrolases / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008361:Mannosidases / Q000172:deficiency / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D043323:alpha-Mannosidase
D006023:Glycoproteins; D006867:Hydrolases; D008361:Mannosidases; D043323:alpha-Mannosidase