Flow cytometry analysis of the growth inhibitory effect of 4-hydroxy-tamoxifen on a human breast carcinoma cell line.
Growth kinetics of BT-20 cells (an ER- cell line) have been studied with flow cytometry, cell counting and 3H-thymidine incorporation. The cell cycle lasts about 40 hours, with a maximum SG2M about 25h after seeding. 4-Hydroxy-tamoxifen (OH-Tamoxifen) (10(-7) M to 10(-5) M) inhibits BT-20 cell growth with maximum inhibition at 24-28h after plating. The inhibitory effect of OH-Tamoxifen is also demonstrated by a decrease in 3H-thymidine incorporation and by a reduction in cell number. These results suggest that OH-Tamoxifen is able to act upon an ER-cell line through a pathway which is different from that of estrogens.
Vicard
E
E
INSERM, Centre Léon Bérard, Lyon, France.
Hijazi
A
A
Muchada
E
E
Chouvet
C
C
Devonec
M
M
Saez
S
S
D001943:Breast Neoplasms / Q000473:pathology*; D002277:Carcinoma / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis; D003864:Depression, Chemical; D004958:Estradiol / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D013629:Tamoxifen; C016601:afimoxifene; D004958:Estradiol