Biosynthesis of 18-hydroxydeoxycorticosterone and corticosterone in adrenal tissue of rat strains with salt-dependent hypertension.
Biosynthetic patterns of 18-OH-deoxycorticosterone (18-OH-DOC) and corticosterone (B) in adrenal tissue of Wistar-rats and two rat strains with salt-dependent hypertension (Dahl-, Sabra-rats) were measured by means of quantitative HPLC-analysis. The relative activity of 18-hydroxylation is expressed as the ratio 18-OH-DOC/18-OH-DOC + B, in the following called Q. Wistar-rats show a constant product ratio Q of 0.40 +/- 0.01. Dahl-rats, however, exhibit typical differences between the two substrains. Adrenal tissue of salt-resistant (Dahl-R) rats produces significantly less 18-OH-DOC than tissue from salt-sensitive (Dahl-S) rats: Q = 0.17 +/- 0.01 (Dahl-R) vs. Q = 0.37 +/- 0.01 (Dahl-S). No such difference was observed between the two Sabra-substrains. This indicates, that other mechanisms than an altered 11 beta-18-hydroxylation are involved in the salt-dependent hypertension of Sabra-rats.
Wohlfeil
S
S
Pharma-Forschungszentrum, BAYER AG, Wuppertal, F.R.G.
Neuser
D
D
Morich
F J
FJ
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis*; D003900:Desoxycorticosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D006973:Hypertension / Q000209:etiology / Q000378:metabolism*; D066298:In Vitro Techniques; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D012965:Sodium Chloride
C042509:18-hydroxydeoxycorticosterone; D003900:Desoxycorticosterone; D012965:Sodium Chloride; D003345:Corticosterone