Demonstration of the adenosine reservoirs with nitrobenzylthioinosine in liver and diaphragm by high-performance liquid chromatography.
Purine nucleotides, nucleosides, nucleobases, dinucleotides and nucleosides derivatives from acid-extracted rat liver and diaphragm were separated and quantitated by reversed-phase ion-pair high-performance liquid chromatography with a mobile phase composed of 90 mM potassium phosphate, 15 mM tetrabutylammonium hydroxide and a 1-30% methanol gradient. During 5 min of ischemia, adenine and guanine nucleotides decreased along with significant declines in NAD and increases in adenosine, inosine, hypoxanthine, xanthine, NADP and adenylosuccinate. Nitrobenzylthioinosine by gavage (5 mg/kg per day for five days) increased adenosine levels but without any alteration in nucleobase levels. Adenosine was shuttled to every available intracellular reservoir which included in declining order of magnitude GDP greater than adenosylhomocysteine greater than adenosine greater than ADP greater than AMP greater than IMP = XMP = GMP.
Jenkins
R L
RL
Department of Medicine, University of Alabama, Birmingham 35294.
McDaniel
H G
HG
Grizzle
W
W
Parrish
S W
SW
McDaniel
H B
HB
D000227:Adenine Nucleotides / Q000032:analysis / Q000378:metabolism; D000241:Adenosine / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003964:Diaphragm / Q000378:metabolism; D007288:Inosine / Q000031:analogs & derivatives*; D007511:Ischemia / Q000378:metabolism; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D012132:Respiratory Muscles / Q000032:analysis / Q000378:metabolism*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013868:Thioinosine / Q000031:analogs & derivatives*
D000227:Adenine Nucleotides; D012997:Solvents; D013868:Thioinosine; D007288:Inosine; C001789:4-nitrobenzylthioinosine; D000241:Adenosine