title: [Inhibition of adrenodoxin reductase by NADP+ and NADPH].
abstract: Adrenodoxin reductase (EC 1.18.1.2) catalyzes the oxidation of NADPH by 1.4-benzoquinone. The catalytic constant of this reaction at pH 7.0 is equal to 25-28 s-1. NADP+ acts as the mixed-type nonlinear inhibitor of enzyme increasing Km of NADPH and decreasing catalytic constant. NADP+ and NADPH act as mutually exclusive inhibitors relative to reduced adrenodoxin reductase. The patterns of 2',5'-ADP inhibition are analogous to that of NADP+. These data support the conclusion about the existence of second nicotinamide coenzyme binding centre in adrenodoxin reductase.
authors.forename: N K
authors.initials: NK
authors.lastname: Chenas
authors.forename: I A
authors.initials: IA
authors.lastname: Martsinkiavichene
authors.forename: Iu Iu
authors.initials: IuIu
authors.lastname: Kulis
authors.forename: S A
authors.initials: SA
authors.lastname: Usanov
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D005287:Ferredoxin-NADP Reductase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D009249:NADP; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases
vernacular_title: Ingibirovanie adrenodoksinreduktazy NADP+ i NADPH.