Changes in ornithine decarboxylase and antizyme activities in developing mouse brain.
A macromolecular inhibitor to ornithine decarboxylase (ODC) present in mouse brain was identified as ODC antizyme [Fong, Heller & Canellakis (1976) Biochim. Biophys. Acta 428, 456-465; Heller, Fong & Canellakis (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 1858-1862] on the basis of kinetic properties, Mr and reversal of its inhibition by antizyme inhibitor. The brain antizyme, however, did not cross-react immunochemically with any of seven monoclonal antibodies to rat liver antizyme. ODC activity in mouse brain rapidly decreased after birth, in parallel with putrescine content, and almost disappeared by 3 weeks of age. Free antizyme activity appeared shortly after birth and increased gradually, whereas ODC-antizyme complex already existed at birth and then gradually decreased. Thus total amount of antizyme remained about the same throughout the developmental period in mouse brain. In addition to ODC-antizyme complex, inactive ODC protein was detected by radioimmunoassay in about the same level as the complex at 3 weeks of age. Upon cycloheximide treatment, both free ODC activity and ODC-antizyme complex rapidly disappeared, although free antizyme and the inactive ODC protein were both quite stable.
Onoue
H
H
Department of Nutrition, Jikei University School of Medicine, Tokyo, Japan.
Matsufuji
S
S
Nishiyama
M
M
Murakami
Y
Y
Hayashi
S
S
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D002850:Chromatography, Gel; D003429:Cross Reactions; D003513:Cycloheximide / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008813:Mice, Inbred ICR; D009955:Ornithine Decarboxylase / Q000276:immunology / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D011506:Proteins; C031017:ornithine decarboxylase antizyme; D003513:Cycloheximide; D009955:Ornithine Decarboxylase