title: The primary structure of chicken liver cytochrome b5 deduced from the DNA sequence of a cDNA clone.
abstract: A cDNA clone encoding the chicken liver cytochrome b5 was isolated by probing a library with synthetic oligonucleotides based on a partial amino acid sequence of the protein. Determination of the DNA sequence indicated a 414-nucleotide open reading frame which encodes a 138-amino acid residue polypeptide. The open reading frame contains 6 amino acids at the amino terminus which were not present on any of the cytochrome b5 polypeptides for which the amino acid sequence has been determined directly, suggesting that the protein is proteolytically processed to the mature form. The results of genomic Southern analysis were consistent with the presence of two structurally different genes in the chicken genome, raising the possibility that the soluble and membrane-bound forms of the protein are the products of separate genes.
authors.affiliation: Genetics Program, Michigan State University, East Lansing 48824.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Somerville
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D003573:Cytochrome b Group / Q000235:genetics* / Q000302:isolation & purification; D015786:Cytochromes b5; D004247:DNA / Q000302:isolation & purification*; D006736:Horses; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
chemical_list: D003573:Cytochrome b Group; D004247:DNA; D015786:Cytochromes b5