title: Autologous red cell agglutination assay for HIV-1 antibodies: simplified test with whole blood.
abstract: An antibody detection procedure based on agglutination of autologous red cells has been developed for samples of whole blood. A nonagglutinating monoclonal antibody to human red blood cells conjugated to a synthetic peptide antigen (in this case residues 579 to 601 of the HIV-1 envelope precursor, Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp- Gly-Cys - Ser-Gly-Lys) permitted the detection of antibodies to the human immunodeficiency virus type 1 (HIV-1) in 10 microliters of whole blood within 2 minutes. Agglutination was specifically inhibited by addition of synthetic peptide antigen but not by unrelated peptides. The frequency of false positive results was 0.1% with HIV-1 seronegative blood donors (n = 874). The false negative results were approximately 1% (n = 81). The autologous red cell agglutination test is potentially suitable for simple, rapid, qualitative screening for antibodies to a variety of antigens of medical and veterinary diagnostic significance.
authors.affiliation: Department of Medicine, University of Melbourne, Repatriation General Hospital, Victoria, Australia.
authors.forename: B E
authors.initials: BE
authors.lastname: Kemp
authors.forename: D B
authors.initials: DB
authors.lastname: Rylatt
authors.forename: P G
authors.initials: PG
authors.lastname: Bundesen
authors.forename: R R
authors.initials: RR
authors.lastname: Doherty
authors.forename: D A
authors.initials: DA
authors.lastname: McPhee
authors.forename: D
authors.initials: D
authors.lastname: Stapleton
authors.forename: L E
authors.initials: LE
authors.lastname: Cottis
authors.forename: K
authors.initials: K
authors.lastname: Wilson
authors.forename: M A
authors.initials: MA
authors.lastname: John
authors.forename: J M
authors.initials: JM
authors.lastname: Khan
mesh_terms: D000372:Agglutination Tests; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D006023:Glycoproteins / Q000276:immunology; D006678:HIV / Q000276:immunology*; D006679:HIV Seropositivity / Q000175:diagnosis*; D006801:Humans; D009842:Oligopeptides / Q000276:immunology; D012191:Retroviridae Proteins / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D009842:Oligopeptides; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins