Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill.
The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3',4',7-tetrahydroxyflavone) and quercetin (3,3',4',5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 microM respectively. These were compared with IC50's values of 5,1000 microM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.
Hsieh
R J
RJ
Lipid Research Laboratory, Cornell University, Ithaca, NY 14853.
German
J B
JB
Kinsella
J E
JE
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001091:Arachidonate Lipoxygenases / Q000037:antagonists & inhibitors*; D005419:Flavonoids / Q000494:pharmacology*; D005880:Gills / Q000201:enzymology*; D016859:Lipoxygenase Inhibitors; D010101:Oxygen Consumption / Q000187:drug effects; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D000975:Antioxidants; D005419:Flavonoids; D016859:Lipoxygenase Inhibitors; D001091:Arachidonate Lipoxygenases