Two transcripts of the same ecdysterone-controlled gene are differentially associated with ribosomes.
The ecdysterone-controlled gene I-18C of Chironomus tentans produces two transcripts by differential splicing: a 1.8-kb RNA, with an open reading frame (ORF) of 417 nucleotides (nt) and a 4.6-kb RNA, with no ORF longer than 270 nt. Centrifugation of cytoplasmic extracts in velocity sedimentation sucrose gradients and CsCl-buoyant-density gradients demonstrates that the 1.8-kb RNA is incorporated into polysomes. The 4.6-kb RNA, however, exhibits characteristics of free ribonucleic acid-protein complexes and monosomes.
Amrein
K
K
Institute for Cell Biology, Swiss Federal Institute of Technology, Zürich, Switzerland.
Dorsch-Häsler
K
K
Lutz
B
B
Lezzi
M
M
D000818:Animals; D002499:Centrifugation, Density Gradient; D002683:Chironomidae / Q000235:genetics*; D046508:Culture Techniques; D004247:DNA / Q000235:genetics; D004175:Diptera / Q000235:genetics*; D004441:Ecdysterone / Q000502:physiology*; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D012270:Ribosomes; D014158:Transcription, Genetic
D004441:Ecdysterone; D004247:DNA