title: Influence of dietary leucine content on the activities of branched-chain amino acid aminotransferase (EC 2.6.1.42) and branched-chain alpha-keto acid dehydrogenase (EC 1.2.4.4) complex in tissues of preruminant lambs.
abstract: 1. Branched-chain amino acid aminotransferase (EC 2.6.1.42; BCAAT) and branched-chain alpha-keto acid dehydrogenase (EC 1.2.4.4; BCKDH) activities were measured preruminant lamb liver, longissimus dorsi muscle, kidney, jejunum and adipose tissue, 2 h after a meal with or without an excess of leucine. 2. Skeletal muscle contained about 70% of the total basal BCAAT activities of the tissues studied whereas liver contained about 60% of the total BCKDH activities of these tissues. 3. BCAAT activities were very low in preruminant lamb tissues. BCKDH was more phosphorylated in tissues of preruminant lambs than in rats, especially in liver. These low catalytic potentialities might contribute to a low rate of branched-chain amino acid catabolism in sheep. 4. Ingestion of an excess of leucine led to an increase in liver and jejunum BCAAT activities and activation of BCKDH in jejunum.
authors.affiliation: Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, Ceyrat, France.
authors.forename: I
authors.initials: I
authors.lastname: Papet
authors.forename: N
authors.initials: N
authors.lastname: Lezebot
authors.forename: F
authors.initials: F
authors.lastname: Barre
authors.forename: M
authors.initials: M
authors.lastname: Arnal
authors.forename: A E
authors.initials: AE
authors.lastname: Harper
mesh_terms: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D004032:Diet; D004789:Enzyme Activation / Q000187:drug effects; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D007930:Leucine / Q000494:pharmacology*; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D014018:Tissue Distribution; D000637:Transaminases / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D012969:Sodium Fluoride; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000637:Transaminases; C019798:branched-chain-amino-acid transaminase; D007930:Leucine