Recovery of malondialdehyde in urine as a 2,4-dinitrophenylhydrazine derivative analyzed with high-performance liquid chromatography.
Malondialdehyde (MDA) in urine was measured as a 2,4-dinitrophenylhydrazine (DNPH) derivative using high-performance liquid chromatography (HPLC) for the analysis. MDA standard coeluted with a peak obtained from rat urine after i.p. administration of MDA standard. This peak was also the only peak containing 14C after injection of a [14C]MDA standard, and was shown by mass spectrometry to contain 1-(2,4-dinitrophenyl)pyrazole, the derivative formed when MDA is treated with DNPH. Depending on the amount given (0.3-5.5 mumol), the recovery (after 24 h sampling period) in urine was 0.7-2.6%. This apparent non-linear kinetics may relate to several factors, such as dose-dependent metabolism. However, the peak urinary concentration approached the expected plasma concentration and reproducible recovery data were obtained, suggesting that MDA was passively excreted in a reasonably stable form. These data indicate that monitoring MDA excretion in urine can give useful information about lipid peroxidation in vivo.
Ekström
T
T
Department of Toxicology, National Institute of Occupational Health, Solna, Sweden.
Garberg
P
P
Egestad
B
B
Högberg
J
J
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D008297:Male; D008314:Malonates / Q000652:urine*; D008315:Malondialdehyde / Q000652:urine*; D013058:Mass Spectrometry; D010659:Phenylhydrazines / Q000652:urine*; D051381:Rats; D011919:Rats, Inbred Strains
D008314:Malonates; D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine; D008315:Malondialdehyde