[Effect of moderately low temperatures (-30 C to -70 C) on the permeability of erythrocyte membranes].
The barrier properties of reconstituted and native erythrocyte membranes frozen to -30, -40 or -70 degrees C and stored for a month were studied. The release of markers, namely haemoglobin molecules, [14C] sucrose and K+ ions from cells and membrane structures was measured. The main changes in the barrier function of ghosts and cells have been found to be due to freeze-thawing rather than to storage conditions. Glycerol, a cryoprotectant, appeared to stabilize the barrier properties of erythrocyte membranes for haemoglobin molecules, [14C] sucrose and to a lesser extent for K+ ions. The cryoprotective effect of glycerol has been shown to be considerably greater towards erythrocytes ghosts than to native erythrocytes.
Kukushkin
A I
AI
Gulevskiĭ
A K
AK
Loevskiĭ
M M
MM
Makarenko
V V
VV
D001793:Blood Preservation; D002463:Cell Membrane Permeability; D003451:Cryoprotective Agents; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005615:Freezing; D006801:Humans
D003451:Cryoprotective Agents
Vliianie umerenno nizkikh temperatur (-30 C do -70 C) na pronitsaemost' membran éritrotsitov.