Specific binding of peanut agglutinin and soybean agglutinin to chondroitinase ABC-digested cartilage proteoglycans: histochemical, ultrastructural cytochemical, and biochemical characterization.
The binding of peanut agglutinin (PNA) and soybean agglutinin (SBA) to cartilage proteoglycans was investigated by histochemical, ultrastructural cytochemical, and biochemical methods. Following aldehyde fixation, specimens of rat epiphyseal cartilage were examined by horseradish peroxidase-labelled lectin cytochemistry with and without prior digestion in chondroitinase ABC. At the light microscope level neither PNA nor SBA exhibited any affinity for cartilage matrix, but became strongly bound following chondroitinase treatment. Similarly, at the ultrastructural level, extracellular matrix granules, presumed to be proteoglycan monomer(s), lacked PNA affinity in undigested specimens, and stained very weakly with SBA. Both PNA and SBA weakly to moderately stained the trans cisternae of the Golgi-flattened cisternae in chondrocytes. The chondrocyte plasmalemma lacked PNA staining, but reacted weakly with SBA. Following chondroitinase digestion, PNA and SBA stained matrix granules, and the cell surface of chondrocytes intensely, whereas the Golgi trans cisternae, the Golgi-derived vacuoles, and multivesicular bodies demonstrated weak to moderate reactivity. Proteoglycan aggregates purified from rat chondrosarcoma and bovine nasal cartilage bound PNA and SBA avidly after digestion with chondroitinase. Undigested proteoglycans lacked affinity for PNA and reacted very weakly with SBA. These results indicate that both PNA and SBA specifically react with chondroitinase-modified oligosaccharide(s) bound to core proteins of cartilage proteoglycans. This provided a specific histochemical and ultrastructural cytochemical procedure for localizing chondroitin sulphate-containing proteoglycans.
Takagi
M
M
Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan.
Saito
I
I
Kuwata
F
F
Otsuka
K
K
D000818:Animals; D002808:Chondroitin Lyases / Q000494:pharmacology*; D002810:Chondroitinases and Chondroitin Lyases / Q000494:pharmacology*; D006132:Growth Plate / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D002808:Chondroitin Lyases; D002810:Chondroitinases and Chondroitin Lyases; D006735:Horseradish Peroxidase; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011509:Proteoglycans; D030262:Soybean Proteins; C017707:Ricinus communis agglutinin-1; C028824:soybean lectin; D010835:Phytohemagglutinins