title: Intracellular records of carbachol-induced theta rhythm in hippocampal slices.
abstract: Intracellular recordings were made in the CA1, CA3 and dentate cell layers prior to, during and after the bath perfusion of 50 microM carbachol on hippocampal slices. Fifty-six percent of the cells in this sample were termed theta (theta)-related, i.e., they exhibited membrane potential oscillations of 5-28 mV and rhythmic spike discharges related to the carbachol-induced extracellular theta-rhythm. The remaining 44% of the cells did not show the above relationships to the extracellular theta-rhythm. Carbachol produced an overall depolarization in all cells, in the range of 10-20 mV. These results demonstrated the cellular basis of carbachol-induced theta in hippocampal slices. This preparation will be a valuable model for studying cellular mechanisms and network properties underlying electroencephalographic activity.
authors.affiliation: Department of Psychology, University of Calgary, Alta., Canada.
authors.forename: B H
authors.initials: BH
authors.lastname: Bland
authors.forename: L V
authors.initials: LV
authors.lastname: Colom
authors.forename: J
authors.initials: J
authors.lastname: Konopacki
authors.forename: S H
authors.initials: SH
authors.lastname: Roth
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D004569:Electroencephalography; D004594:Electrophysiology; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013826:Theta Rhythm
chemical_list: D002217:Carbachol