title: Calcium sensitivity of microtubules changes during the cell cycle of Xenopus laevis tadpole endothelial cells.
abstract: Endothelial cells derived from Xenopus tadpole hearts (XTH-2: Schlage et al. 1981) have been permeabilized for calcium using the ionophore A 23187. A Ca2+-concentration of 1 microM causes a graded disassembly of microtubules (mts): with increasing periods of incubation mts disintegrate and then depolymerize completely. The frequency of the different stages of depolymerization depends on the length of the Ca2+ incubation period, even after 60 min incubation time some mts remain. The stage of depolymerization of mts in a cell is correlated with its cell cycle phase showing the highest stability during S phase.
authors.affiliation: Cinematic Cell Research Group, J.W. Goethe-University, Frankfurt a.M., Germany.
authors.forename: B
authors.initials: B
authors.lastname: Stolz
authors.forename: J
authors.initials: J
authors.lastname: Bereiter-Hahn
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002453:Cell Cycle; D004247:DNA / Q000032:analysis; D004727:Endothelium; D007399:Interphase; D008870:Microtubules / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000648:ultrastructure; D013997:Time Factors; D014982:Xenopus laevis
chemical_list: D004247:DNA; D002118:Calcium