Chitinase in female Onchocerca gibsoni and its inhibition by allosamidin.
Chitinase activity has been detected in female worms of Onchocerca gibsoni. With 3,4-dinitrophenyl-tetra-N-acetylchitotetraoside as substrate 50% of maximum activity was achieved at about 25 microM substrate, with inhibition above 50 microM substrate. The antibiotic allosamidin very strongly inhibited the chitinase activity, 50% inhibition being achieved by 200 pM allosamidin in the presence of 45 microM substrate.
Gooday
G W
GW
Department of Genetics and Microbiology, University of Aberdeen, U.K.
Brydon
L J
LJ
Chappell
L H
LH
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005260:Female; D005944:Glucosamine / Q000031:analogs & derivatives*; D009854:Onchocerca / Q000201:enzymology*; D014312:Trisaccharides
D014312:Trisaccharides; C054851:allosamidin; D002688:Chitinases; D005944:Glucosamine; D000117:Acetylglucosamine