Identification of male bloodstains by dot hybridization of human Y chromosome-specific deoxyribonucleic acid (DNA) probe.
The sex determination of bloodstains was performed using a human Y chromosome-specific (DNA) fragment of 1.9-kb length as a hybridization probe. The DNA samples were taken from 1- and 4-week-old bloodstains of males and females, respectively. Strong signals with male DNA were observed by Y-probe, while faint signals with female DNA were detected. In addition, clear signals were observed in the extract samples from male bloodstains (16-week-old) on paper. Dot hybridization of the Y-probe would be widely applicable to studies on sex determination of medicolegal materials such as blood, bloodstains, teeth, and cadaverous parts.
Fukushima
H
H
Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Hasekura
H
H
Nagai
K
K
D001801:Blood Stains; D004247:DNA / Q000097:blood*; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012732:Sex Determination Analysis; D014998:Y Chromosome
D004274:DNA, Recombinant; D004247:DNA