title: Validity testing of the TDx Cocaine Metabolite Assay with human specimens obtained after intravenous cocaine administration.
abstract: Clinical specimens obtained from human subjects after intravenous cocaine administration were analyzed by the TDx Cocaine Metabolite Assay (TDx) and by GC/MS for benzoylecgonine. The TDx results were significantly correlated with results by GC/MS assay with no evidence of bias in the TDx assay. All cocaine metabolite positive specimens (greater than or equal to 300 ng/ml) were confirmed by GC/MS. Detection times to the last positive specimen by TDx assay and GC/MS assay of four subjects after a 20-mg intravenous dose of cocaine ranged from 29.3 to 39.1 h and 27.9 and 36.6 h, respectively. Overall, the TDx assay was found to be highly specific and accurate for the detection and measurement of benzoylecgonine in urine.
authors.affiliation: National Institute on Drug Abuse, Addiction Research Center, Baltimore, MD 21224.
authors.forename: E J
authors.initials: EJ
authors.lastname: Cone
authors.forename: S L
authors.initials: SL
authors.lastname: Menchen
authors.forename: J
authors.initials: J
authors.lastname: Mitchell
mesh_terms: D003042:Cocaine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D019966:Substance-Related Disorders / Q000652:urine*; D013997:Time Factors
chemical_list: C005618:benzoylecgonine; D003042:Cocaine