title: An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects.
abstract: We have developed an in vitro muscle preparation suitable for metabolic studies with human muscle tissue and have investigated the effects of obesity and non-insulin-dependent diabetes mellitus (NIDDM) on glucose transport. Transport of 3-O-methylglucose and 2-deoxyglucose was stimulated approximately twofold by insulin in muscle from normal nonobese subjects and stimulation occurred in the normal physiological range of insulin concentrations. In contrast to insulin stimulation of 3-O-methylglucose and 2-deoxyglucose transport in muscle from normal, nonobese subjects, tissue from morbidly obese subjects, with or without NIDDM, were not responsive to insulin. Maximal 3-O-methylglucose transport was lower in muscle of obese than nonobese subjects. Morbidly obese patients, with or without NIDDM, have a severe state of insulin resistance in glucose transport. The novel in vitro human skeletal muscle preparation herein described should be useful in investigating the mechanism of this insulin resistance.
authors.affiliation: Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.
authors.forename: G L
authors.initials: GL
authors.lastname: Dohm
authors.forename: E B
authors.initials: EB
authors.lastname: Tapscott
authors.forename: W J
authors.initials: WJ
authors.lastname: Pories
authors.forename: D J
authors.initials: DJ
authors.lastname: Dabbs
authors.forename: E G
authors.initials: EG
authors.lastname: Flickinger
authors.forename: D
authors.initials: D
authors.lastname: Meelheim
authors.forename: T
authors.initials: T
authors.lastname: Fushiki
authors.forename: S M
authors.initials: SM
authors.lastname: Atkinson
authors.forename: C W
authors.initials: CW
authors.lastname: Elton
authors.forename: J F
authors.initials: JF
authors.lastname: Caro
mesh_terms: D019325:3-O-Methylglucose; D000328:Adult; D001692:Biological Transport / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D006801:Humans; D066298:In Vitro Techniques; D007333:Insulin Resistance; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D009132:Muscles / Q000378:metabolism* / Q000648:ultrastructure; D009767:Obesity, Morbid / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
chemical_list: D008757:Methylglucosides; D019325:3-O-Methylglucose; D003847:Deoxyglucose; D005947:Glucose