Role of myometrial constriction in the induction of wavy ribs in rat fetuses.
Furosemide, a loop diuretic, was orally administered to pregnant Crj: CD (SD) rats at a dose of 300 mg/kg once on day 16 of gestation. Cartilage bone double staining of day 17 fetuses revealed a delay in ossification. Characteristic rib deformities were not seen until day 18 of gestation. Concomitant with this observation were reductions in the amount of amniotic fluid, compared to controls, on day 18 of gestation and thereafter. When isoxsuprine HCl (2 mg/kg, b.i.d.), a uterine muscle relaxant, was additionally injected ip to pregnant rats on days 17-19 of gestation, the incidence of wavy ribs was significantly (P less than or equal to .05) less than that seen after treatment with furosemide alone. These observations indicate that the myometrial constriction plays a significant role in the induction of furosemide-induced wavy ribs.
Nakatsuka
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Department of Teratology, Banyu Pharmaceutical Co., Ltd., Saitama, Japan.
D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005665:Furosemide / Q000633:toxicity*; D007556:Isoxsuprine / Q000494:pharmacology; D009215:Myometrium / Q000503:physiopathology*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012272:Ribs / Q000002:abnormalities*
D005665:Furosemide; D007556:Isoxsuprine