title: Isolation of cDNA clones of human argininosuccinate lyase and corrected amino acid sequence.
abstract: In the present study, we isolated clones of human argininosuccinate lyase (ASL) cDNA from a liver cDNA library using a clone of rat ASL cDNA and analyzed human ASL cDNA nucleotide sequence. The results reveal that the sequence of human ASL cDNA published by O'Brien et al. in 1986 [Proc. Natl. Acad. Sci USA 83, 7211-7215] had one-base deletions at three independent positions in the coding regions near the COOH-terminus, which caused frame-shift variations in the amino acid sequence. Amino acid sequencing of peptides prepared from purified human liver ASL showed our predicted amino acid sequence to be correct.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Kagoshima Univeristy, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matuo
authors.forename: M
authors.initials: M
authors.lastname: Tatsuno
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Saheki
authors.forename: T
authors.initials: T
authors.lastname: Miyata
authors.forename: S
authors.initials: S
authors.lastname: Iwanaga
authors.forename: Y
authors.initials: Y
authors.lastname: Amaya
authors.forename: M
authors.initials: M
authors.lastname: Mori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001123:Argininosuccinate Lyase / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005796:Genes; D006801:Humans; D008099:Liver / Q000201:enzymology; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats
chemical_list: D004247:DNA; D008190:Lyases; D001123:Argininosuccinate Lyase