Carrier-mediated uptake of L-(+)-lactate in plasma membrane vesicles from rat liver.
Plasma membrane vesicles from rat liver transported L-lactate into the inner vesicular space. Kinetic analysis of L-lactate uptake gave a Km value of approx. 2.9 mM. Selective inhibition was found in a similar pattern to that described for the hepatic lactate carrier. L-Lactate transport was enhanced when a pH gradient was created across the plasma membrane. Vesicles obtained from fasted rats showed a higher uptake of L-lactate than those from fed rats, when incubated with physiological concentrations of L-lactate.
Quintana
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I
Departmento de Bioquimica y Fisiologia, Facultad de Biologia, Universidad de Barcelona, Spain.
Felipe
A
A
Remesar
X
X
Pastor-Anglada
M
M
D000409:Alanine / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology; D005215:Fasting; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008099:Liver / Q000378:metabolism*; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains
D002352:Carrier Proteins; D003373:Coumaric Acids; D007773:Lactates; D019344:Lactic Acid; C018254:alpha-cyano-3-hydroxycinnamate; D000409:Alanine