Induction of an antibody that catalyzes the hydrolysis of an amide bond.
Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.
Janda
K D
KD
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Schloeder
D
D
Benkovic
S J
SJ
Lerner
R A
RA
D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000502:physiology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D006433:Hemocyanins / Q000031:analogs & derivatives / Q000276:immunology; D006868:Hydrolysis; D007114:Immunization; D007700:Kinetics; D051379:Mice; D009943:Organophosphorus Compounds / Q000276:immunology; D013379:Substrate Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens; D009943:Organophosphorus Compounds; C056997:keyhole limpet hemocyanin phosphonamidate conjugate; D006433:Hemocyanins; D000581:Amidohydrolases; C032808:keyhole-limpet hemocyanin