Identification of a complex of the three forms of the rat liver asialoglycoprotein receptor.
We have generated antibodies against synthetic peptides which represent the carboxyl terminus of either the major, or the two minor, forms of the rat hepatic lectin which recognizes galactose-terminated glycoproteins (asialoglycoproteins). The antibodies were shown to be specific for the form of the lectin containing the immunizing peptide sequence by the following: reaction with purified lectin after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoprecipitation of sodium dodecyl sulfate-denatured lectin, immunoprecipitation of lectin synthesized in vitro. These antibodies, however, precipitated all three rat hepatic lectin forms from nonionic detergent extracts of hepatocytes labeled with 125I via the lactoperoxidase catalyzed technique. A similar result was obtained if antibody was bound to intact cells prior to solubilization with detergent and collection of the immune complexes. We conclude that at least the plasma membrane-associated fraction of the rat hepatic lectin forms exists as a heterotypic complex.
Sawyer
J T
JT
Department of Biological Sciences, State University of New York, Buffalo 14260.
Sanford
J P
JP
Doyle
D
D
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D037263:Asialoglycoprotein Receptor; D004797:Enzyme-Linked Immunosorbent Assay; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D011971:Receptors, Immunologic / Q000276:immunology* / Q000302:isolation & purification
D000906:Antibodies; D000936:Antigen-Antibody Complex; D037263:Asialoglycoprotein Receptor; D011971:Receptors, Immunologic