A new europium chelate for protein labelling and time-resolved fluorometric applications.
Synthetic procedures are presented for a new chelator that forms stable and highly fluorescent complexes with Eu3+. This chelator, 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) is synthesized in a high-yield three-step procedure. BCPDA can be covalently incorporated into proteins under relatively mild conditions, and when complexed with Eu3+ forms a fluorescent product that has a lifetime in the range of 0.4 to 0.7 ms. Thus, it is useful for time-resolved fluorescence immunoassay applications.
Evangelista
R A
RA
HSC Research Development Corporation, University of Toronto, Ontario, Canada.
Pollak
A
A
Allore
B
B
Templeton
E F
EF
Morton
R C
RC
Diamandis
E P
EP
D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000138:chemical synthesis; D005063:Europium; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D009682:Magnetic Resonance Spectroscopy; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared
D002614:Chelating Agents; D005456:Fluorescent Dyes; D011506:Proteins; D012710:Serum Albumin, Bovine; D005063:Europium