Influence of vincristine on the Golgi apparatus in preimplantation development of the mouse embryo.
The aim of our study was the evaluation of the action of vincristine (VCR) on the Golgi apparatus of mouse embryos from females receiving this drug. Quantitative and qualitative changes were investigated in 1-, 2-, 4-, and 8-cell embryos, morulae, and blastocysts from female mice treated with VCR in the amount of 0.075 mg/kg body weight 5 times once weekly. The number of embryos was decreased in all the examined developmental stages of preimplantation development. Electron microscopic investigation demonstrated translocation and dispersion of the Golgi apparatus components and changes in their relative volume as compared with that of control animals. The widest changes were noted on the 4-, 8-cell embryos and morulae in the experimental group. In the blastocyst stage, statistically significant differences in the Golgi apparatus were not demonstrated between the experimental and the control group. The present results seem to suggest the existence of remote effects of VCR which may influence the development of the progeny of females treated with this drug.
Barańska
W
W
Laboratory of Electron Microscopy, Institute of Biostructure Medical Academy, Warsaw, Poland.
Kujawa
M
M
Kujawska
E
E
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000648:ultrastructure*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D006056:Golgi Apparatus / Q000187:drug effects / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D009028:Morula / Q000187:drug effects / Q000648:ultrastructure; D014750:Vincristine / Q000494:pharmacology*
D006063:Chorionic Gonadotropin; D014750:Vincristine