Cytotoxic activity in broth-culture filtrates of Campylobacter pylori.
Broth-culture filtrates of Campylobacter pylori induced non-lethal cytopathic effects in vitro in 7 of 9 mammalian cell lines tested. Transmission electronmicroscopy revealed that the response consisted of intracellular vacuolisation. Intestine 407 cells were among the most responsive and were used for routine assay. About 55% of isolates of C. pylori tested, originating from four geographic regions worldwide, produced cytotoxic activity. The activity was neutralisable by specific antisera to broth-culture filtrates or to sonicated bacteria but not by antisera to other bacterial preparations. Cytotoxic activity was heat-labile (70 degrees C for 30 min), was protease-sensitive and ammonium-sulphate precipitable. It did not pass through an ultrafiltration membrane with a nominal mol.-wt limit of 100 X 10(3). It was concluded that C. pylori can produce a factor that alters cultured cells in vitro. The relevance of this factor to the pathogenesis of gastritis associated with C. pylori remains to be determined.
Leunk
R D
RD
Anti-Infective Research Department, Norwich Eaton Pharmaceuticals Inc., New York 13815.
Johnson
P T
PT
David
B C
BC
Kraft
W G
WG
Morgan
D R
DR
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002167:Campylobacter / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D002460:Cell Line; D003470:Culture Media; D003603:Cytotoxins / Q000096:biosynthesis*; D006367:HeLa Cells; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D011817:Rabbits; D014617:Vacuoles / Q000648:ultrastructure; D014709:Vero Cells
D001427:Bacterial Toxins; D003470:Culture Media; D003603:Cytotoxins; D007106:Immune Sera