A radioimmunohistochemical method for autoradiographic visualization of cell antigens using 125I-staphylococcal protein A.
125I-Staphylococcal protein A was used to visualize immunoreactive cell antigen in rat brain and pituitary by autoradiography. Autoradiograms of rat brain sections generated with 125I-protein A were clear and showed low background signals. We were able to visualize neural structures containing tyrosine hydroxylase or methionine-enkephalin-like immunoreactivities in the brain, and vasopressin-like immunoreactivity in the pituitary gland. Our results suggest that 125I-protein A can be used for the radioimmunohistochemical visualization of cell antigens in tissue sections.
Correa
F M
FM
Unit on Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, MD 20892.
Guilhaume
S S
SS
Saavedra
J M
JM
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001345:Autoradiography; D001923:Brain Chemistry; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D008297:Male; D010902:Pituitary Gland / Q000032:analysis*; D051381:Rats; D013205:Staphylococcal Protein A
D000941:Antigens; D007457:Iodine Radioisotopes; D013205:Staphylococcal Protein A