High-resolution anion exchange chromatography of the glucocorticoid receptor from WEHI-7 cells.
A simple refinement of the current methods of DEAE chromatography of steroid receptors has been developed which takes advantage of the characteristics of Fast Flow DEAE Sepharose (Pharmacia). The approach provides a convenient and inexpensive means to carry out high-resolution chromatography of the glucocorticoid receptor. Using this method, at least five separate species of receptor have been detected within the single so-called "low-salt" peak normally seen using the current methods of receptor anion exchange chromatography.
Gruol
D J
DJ
Salk Institute for Biological Studies, San Diego, CA 92138.
Campbell
N F
NF
Bourgeois
S
S
D000818:Animals; D002460:Cell Line; D002852:Chromatography, Ion Exchange / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification*
D011965:Receptors, Glucocorticoid