title: A dye release assay for determination of lysostaphin activity.
abstract: We describe a method for determination of lysostaphin activity using Remazol Brilliant Blue R (RBB)-dyed staphylococcal cells or RBB-dyed staphylococcal peptidoglycan as substrate. The dyed substrates are easy to prepare and are stable for at least 6 months. Soluble hydrolytic products released by lysostaphin are measured spectrophotometrically at 595 nm after the insoluble substrate is removed by filtration or centrifugation. The dye release assay is more sensitive and more accurate than the previously described turbidimetric assay.
authors.affiliation: Public Health Research Institute, New York, New York 10016.
authors.forename: R
authors.initials: R
authors.lastname: Zhou
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: P
authors.initials: P
authors.lastname: Recsei
mesh_terms: D000880:Anthraquinones; D002473:Cell Wall / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008248:Lysostaphin / Q000032:analysis*; D010457:Peptidoglycan / Q000032:analysis; D012995:Solubility; D013210:Staphylococcus / Q000201:enzymology
chemical_list: D000880:Anthraquinones; D010457:Peptidoglycan; D008248:Lysostaphin; C024758:Remazol Brilliant Blue R