Stable isotope evidence for the localisation of phenylalanine biosynthesis in the bacterium Paracoccus denitrificans.
Paracoccus denitrificans was grown on [6-13C]-glucose as the sole carbon source for growth and the extracts were fractionated and analysed by gas chromatography-mass spectrometry. The 13C-labelling pattern observed in phenylalanine indicated that the biosynthetic sequences of enzymes for phenylalanine production were unequally distributed within the cell and that there are at least 2 separate loci of phenylalanine biosynthesis. The principal locus of phenylalanine production was associated with the Entner-Doudoroff and/or the pentose phosphate pathways and it was responsible for producing 3/4 of the bacterium's phenylalanine. A second locus was associated with the G6 oxidation pathway and was responsible for producing the remaining 1/4 of the cell's phenylalanine.
Dunstan
R H
RH
Department of Plant Sciences, University of Oxford, U.K.
Whatley
F R
FR
Greenaway
W
W
D002247:Carbon Isotopes; D005947:Glucose / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D010649:Phenylalanine / Q000096:biosynthesis*; D010728:Phosphoenolpyruvate / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism
D002247:Carbon Isotopes; D013403:Sugar Phosphates; D010649:Phenylalanine; C026959:erythrose 4-phosphate; D010728:Phosphoenolpyruvate; D005947:Glucose