Rates of muscle protein breakdown in chickens selected for increased growth rate, food consumption or efficiency of food utilisation as assessed by N tau-methylhistidine excretion.
1. N tau-methylhistidine excretion, growth rate, food consumption and body composition was determined in 12 4 to 5 week old chickens sampled from each of 4 lines selected for increased body-weight gain (line W), for increased food consumption (line F), for improved efficiency of food utilisation (line E) or at random (line C), after 12 generations of selection. 2. The use of N tau-methylhistidine as an index of myofibrillar protein breakdown was validated in male and female chickens of lines E and F by following the fate of injected N tau-(14CH3)methylhistidine. Most of the radioactivity (79.3 +/- 1.1%) was excreted in 4 d, with the remainder retained in the carcase. In excreta, 94 +/- 2% of the radioactivity was associated with free N tau-methylhistidine and for the carcase, this value was 88 +/- 3%. 3. In the main experiment, final body weights averaged 497, 651, 588 and 537 g and food: gain ratio averaged 2.47, 2.21, 3.14 and 2.06 for lines C, W, F and E respectively, Carcase protein content (g/100 g body weight) was not different between the lines. 4. N tau-methylhistidine excretion was 5.86, 5.48, 6.43 and 4.99 mumoles/mole carcase-N/d for lines C, W, F and E, respectively. The rate for line F was significantly higher than for lines W and C and that for line E was significantly less than for the control line. 5. The N tau-methylhistidine excretion rate was positively correlated with food: gain ratio. 6. Selection for rapid growth, high food consumption or improved food utilisation results in changes in N tau-methylhistidine excretion which suggest proportionate changes in muscle protein turnover.
Tomas
F M
FM
CSIRO, Division of Human Nutrition, Adelaide, South Australia.
Jones
L M
LM
Pym
R A
RA
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001823:Body Composition; D002645:Chickens / Q000378:metabolism*; D005243:Feces / Q000032:analysis; D005260:Female; D006639:Histidine / Q000031:analogs & derivatives*; D008297:Male; D008762:Methylhistidines / Q000032:analysis / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D012641:Selection, Genetic
D008762:Methylhistidines; D009124:Muscle Proteins; D006639:Histidine; C028118:3-methylhistidine