title: Processing of two protein precursors yields four mature guinea pig seminal vesicle secretory proteins.
abstract: The translation of the two most abundant guinea pig seminal vesicle epithelium mRNAs (1800 nucleotides and 950 nucleotides) and the subsequent processing of their protein products were studied in an effort to elucidate the mechanism by which the four mature guinea pig seminal vesicle epithelium (GPSVE) secretory proteins are produced. The primary translation products of the 1800 nt and 950 nt mRNAs are two secretory protein precursors of 45 kDa and 20 kDa, respectively. Removal of signal peptides from these two precursors produces proteins of 43 kDa and 18.5 kDa, which are recognized by polyclonal antisera directed against the four mature secretory proteins. The existence of further processing intermediates in the production of the secretory proteins is suggested by the appearance of other immunoreactive polypeptides following incubation of GPSVE in nutrient medium containing [3H] leucine. Immunological and pulse-chase analysis strongly suggests that the 43-kDa protein gives rise to SVP-1, -3, and -4 and that SVP-2 is derived from the 18.5-kDa protein.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, Minnesota 55905.
authors.forename: M E
authors.initials: ME
authors.lastname: Norvitch
authors.forename: S
authors.initials: S
authors.lastname: Harvey
authors.forename: J T
authors.initials: JT
authors.lastname: Moore
authors.forename: E D
authors.initials: ED
authors.lastname: Wieben
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000378:metabolism; D006168:Guinea Pigs; D007118:Immunoassay; D008297:Male; D029584:Prostatic Secretory Proteins; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000378:metabolism*
chemical_list: D029584:Prostatic Secretory Proteins; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011506:Proteins; D012333:RNA, Messenger; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein