Miniaturization of the immunoblot technique. Rapid screening for the detection of monoclonal and polyclonal antibodies.
A miniaturization of the immunoblot technique permitting the use of as little as 50 microliters of antibody is described. This method does not require any special equipment and the whole experimental procedure (incubation, washing, detection) can be performed in the same chamber, with economy of reagents at all steps and no prenumbering of blotted strips. This method has been applied for screening the presence of antibodies in hybridoma culture supernatants and is of special interest for testing any scarce material (immune sera or antigens).
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Neurobiologie Moléculaire, Institut Pasteur, Paris, France.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D007106:Immune Sera / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008808:Mice, Inbred CBA; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D010209:Paper
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D003101:Collodion