Mode of action of deoxypheganomycin D on Mycobacterium smegmatis ATCC 607.
Deoxypheganomycin D, a specific inhibitor of mycobacteria, inhibits the growth in vitro of Mycobacterium smegmatis ATCC 607 (M. 607) bacteriostatically at concentrations as high as 7 X 10(-5) M. It shows no cross-resistance to paromomycin, capreomycin, viomycin, streptothricin, kanamycin and streptomycin. Deoxypheganomycin D at 2.8 X 10(-7) M where the cell growth of M. 607 is only partially inhibited does not significantly inhibit DNA, RNA or protein synthesis but leads to marked decrease (13% of control) in [14C]glycerol-derived radioactivity in cell-walls. In the presence of 7 X 10(-6) M deoxypheganomycin D, the influx of leucine but not thymidine is affected while the reverse is true with efflux. The data suggest that the effect of deoxypheganomycin D on M. 607 may be related to both the cell membrane and specific mycobacterial lipid like components of the cell-wall.
Suzukake-Tsuchiya
K
K
Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Hori
M
M
Shimada
N
N
Hamada
M
M
D000596:Amino Acids / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins / Q000096:biosynthesis; D002463:Cell Membrane Permeability / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development; D009694:Nucleic Acid Precursors / Q000378:metabolism; D010455:Peptides
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009694:Nucleic Acid Precursors; D010455:Peptides; C056343:deoxypheganomycin D