Signals controlling alternative splicing of major histocompatibility complex H-2 class I pre-mRNA.
The use of alternative splice acceptor sites during the removal of intron 7 in pre-mRNA splicing produces two forms of H-2Kb protein: the predominant form, derived from a transcript that has spliced at the upstream splice acceptor site for exon 8 (long exon 8), and a Kb molecule derived from a transcript that has spliced at the downstream acceptor site for exon 8 (short exon 8). We have identified a potential lariat branch point adenosine for the upstream acceptor splice site. This adenosine is found 28 bp from the splice junction and is contained in the sequence AGTGATGG. D-region genes, which use only the downstream splice site, have the sequence AGTGGTGG. We have used in vitro mutagenesis to change this A of the H-2Kb gene to G and have made the reciprocal change in H-2Dd. Elimination of this adenosine in H-2Kb alters the pattern of pre-mRNA splicing and results in a predominance of the Kb molecules with short exon 8 encoded sequences. However, the addition of an adenosine in H-2Dd is not sufficient to direct splicing to the upstream site.
Handy
D E
DE
Biological Resources Branch, National Institutes of Health, Bethesda, MD 20892.
McCluskey
J
J
Lew
A M
AM
Coligan
J E
JE
Margulies
D H
DH
D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000276:immunology; D004252:DNA Mutational Analysis; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I; D006183:H-2 Antigens / Q000235:genetics*; D007438:Introns; D007739:L Cells; D051379:Mice; D009694:Nucleic Acid Precursors / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D014162:Transfection
D006183:H-2 Antigens; D009694:Nucleic Acid Precursors; D012333:RNA, Messenger