Receptor analysis: an arithmetic correction improves precision and accuracy.
Data of receptor analysis by ligand binding experiments should be processed using the formula DCORR = (B1 - B2.F1/F2)/VS.DCORR is an estimate of the concentration of receptor-bound radioligand; B1 and F1 are estimates of bound and free radioligand in assay 1; B2 and F2 are the corresponding values obtained from the parallel assay 2, which contains an additional excess of nonlabeled ligand; VS is the volume of assays 1 and 2 that was submitted to separation. DCORR will be superior to the conventional formula, D = (B1 - B2)/VS, if the radiolabeled receptor-ligand complexes are incompletely separated from nonspecifically bound and free radioligands. DCORR corrects for the systematic underestimation of the specifically bound radioligand implicated in D as well as for random errors due to imprecise pipetting during preparation of the parallel assays. The superiority of DCORR over D is verified by processing the data of androgen receptor analyses using agar gel electrophoresis for separation of bound and free radioligand.
Oettling
G
G
Institute of Anatomy, University of Tübingen, Federal Republic of Germany.
Moeller
H
H
D000818:Animals; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008433:Mathematics; D008954:Models, Biological; D011467:Prostate / Q000032:analysis; D011869:Radioligand Assay; D051381:Rats; D011944:Receptors, Androgen / Q000032:analysis
D011944:Receptors, Androgen