Survival and development of embryonic and postnatal cerebellum transplanted into adult rat hosts: effect of growth as explants in culture prior to transplantation.
The survival and organization of embryonic and postnatal cerebellum transplanted into intracephalic cavities in adult rat hosts was studied before and after growth as explants in tissue culture. It is well known that embryonic cerebellum survives transplantation and develops a typical cerebellar cytoarchitecture. This study demonstrates that early postnatal cerebellum survives transplantation poorly if at all using the intracephalic transplant paradigm. Postnatal cerebellum is capable of extensive growth and organization in explant culture; however, prior growth in explant culture did not enhance survival in the transplant situation. On the other hand, embryonic cerebellum grown in explant culture for as long as two weeks, to an age comparable to the postnatal cerebellar transplants, survives transplantation and develops similar cytoarchitectural features to directly transplanted embryonic cerebellum.
Ezerman
E B
EB
Department of Anatomy and Neurobiology, University of Vermont, College of Medicine, Burlington 05405.
D000375:Aging; D000818:Animals; D002470:Cell Survival; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000637:transplantation*; D006085:Graft Survival; D009924:Organ Culture Techniques; D051381:Rats; D013997:Time Factors