[Ortho-dianizidine micromethod of determining L-phenylalanine-alpha-oxidase activity].
A micromethod is developed for estimation of 1-phenylalanine-alpha-oxidase activity from Trichoderma sp. The method is based on interaction of H2O2 formed in the reaction with o-dianisidine.HCl. Optimal conditions were adopted for estimation of the new enzyme activity from Trichoderma sp. The enzyme had Km = 2.5.10(-3) M and Vmax = 0.42 U/mg at 20 mM concentration of phenylalanine in the incubation mixture; the highest enzymatic activity was estimated in citrate buffer, pH 5.0. The method is simple, precise and reproducible.
Smirnova
I P
IP
Berezov
T T
TT
D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D001560:Benzidines; D003962:Dianisidine; D006863:Hydrogen-Ion Concentration; D008722:Methods; D003904:Mitosporic Fungi / Q000201:enzymology*; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology*
D001560:Benzidines; D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase; D003962:Dianisidine
Ortodianizidinovyĭ mikrometod opredeleniia aktivnosti L-fenilalanin-alpha-oksidazy.