Immunocytochemical localization of glutamate dehydrogenase in mitochondria of the cerebellum: an ultrastructural study using a monoclonal antibody.
Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degrees of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and astrocytes in the cerebellum of the rat. Differential intensity of staining among mitochondria within individual glial cells and between glial cells was observed and may provide an anatomical means of detecting differences in glutamate metabolism.
Madl
J E
JE
Department of Veterinary Biology, University of Minnesota, St. Paul 55108.
Clements
J R
JR
Beitz
A J
AJ
Wenthold
R J
RJ
Larson
A A
AA
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002531:Cerebellum / Q000201:enzymology* / Q000648:ultrastructure; D005969:Glutamate Dehydrogenase / Q000032:analysis*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008928:Mitochondria / Q000201:enzymology*; D051381:Rats
D000911:Antibodies, Monoclonal; D005969:Glutamate Dehydrogenase