title: Presence of epoxide hydrolase and glutathione S-transferase in human pulmonary alveolar macrophages.
abstract: Pulmonary alveolar macrophages (PAMs) were obtained from 11 patients by bronchoalveolar lavage. Epoxide hydrolase and glutathione S-transferase in sonicated PAMs were measured using benzo(a)pyrene-4,5-oxide as the substrate. The activity of epoxide hydrolase was 0.24 +/- 0.10 nmol/min/mg protein (mean +/- SD), and of glutathione S-transferase 0.22 +/- 0.12 nmol/min/mg protein. There was a significant difference in enzyme activities in the PAMs from smokers and non-smokers. Epoxides may be metabolized in PAMs.
authors.affiliation: Institute of 2nd Medical Clinic, University of Pisa, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Petruzzelli
authors.forename: P
authors.initials: P
authors.lastname: Bernard
authors.forename: P
authors.initials: P
authors.lastname: Paoletti
authors.forename: A
authors.initials: A
authors.lastname: Rane
authors.forename: C
authors.initials: C
authors.lastname: Giuntini
authors.forename: G M
authors.initials: GM
authors.lastname: Pacifici
mesh_terms: D004851:Epoxide Hydrolases / Q000378:metabolism*; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D011650:Pulmonary Alveoli / Q000166:cytology / Q000201:enzymology*
chemical_list: D005982:Glutathione Transferase; D004851:Epoxide Hydrolases