title: A microelectrophoretic method for the evaluation of GABA transaminase activity.
abstract: The reduction to the micro-scale of a recently described electrophoretic method for the evaluation of GABA catabolism by GABA-T is presented. The micromethod involves the electrophoresis, in 1 mm diam. capillaries, of small samples of mixtures of [14C]GABA and its metabolites. By coupling this procedure to previously devised micromethods, it was possible to evaluate GABA-T attack to 14C labeled GABA diffusing across a single microdissected neuronal membrane.
authors.affiliation: Centro di Neurofisiologia Cerebrale, C.N.R., Genova, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cupello
authors.forename: S
authors.initials: S
authors.lastname: Larsson
authors.forename: A
authors.initials: A
authors.lastname: Palm
authors.forename: M V
authors.initials: MV
authors.lastname: Rapallino
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000032:analysis*; D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D066298:In Vitro Techniques; D009474:Neurons / Q000201:enzymology*; D011817:Rabbits; D014726:Vestibular Nuclei / Q000201:enzymology*
chemical_list: D000612:4-Aminobutyrate Transaminase