A microelectrophoretic method for the evaluation of GABA transaminase activity.
The reduction to the micro-scale of a recently described electrophoretic method for the evaluation of GABA catabolism by GABA-T is presented. The micromethod involves the electrophoresis, in 1 mm diam. capillaries, of small samples of mixtures of [14C]GABA and its metabolites. By coupling this procedure to previously devised micromethods, it was possible to evaluate GABA-T attack to 14C labeled GABA diffusing across a single microdissected neuronal membrane.
Cupello
A
A
Centro di Neurofisiologia Cerebrale, C.N.R., Genova, Italy.
Larsson
S
S
Palm
A
A
Rapallino
M V
MV
D000612:4-Aminobutyrate Transaminase / Q000032:analysis*; D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D066298:In Vitro Techniques; D009474:Neurons / Q000201:enzymology*; D011817:Rabbits; D014726:Vestibular Nuclei / Q000201:enzymology*
D000612:4-Aminobutyrate Transaminase