Identification of protein A from Staphylococcus intermedius isolated from canine skin.
Protein A was identified in cell wall-bound and secreted forms from Staphylococcus intermedius isolated from canine skin. A direct binding radioimmunoassay for the detection of bacterial surface Fc receptors identified 48 of 50 S intermedius isolates that contained cell wall-bound protein A. Using a competitive binding radioimmunoassay for the detection of Fc-reactive proteins in bacterial culture supernatants, we identified 9 of 50 clinical isolates of S intermedius that secreted measurable quantities of an Fc receptor into the culture medium. Concentrated culture supernatants from these isolates were analyzed by western blotting techniques and probed with either a radiolabeled human IgG Fc-specific probe or a radiolabeled affinity-purified chicken antibody against protein A. The studies reported here confirmed that Fc receptors are secreted by S intermedius isolates from dogs and are antigenically and functionally similar or are identical to staphylococcal protein A. Analysis of Fc receptor secretion by S intermedius strains, isolated from dogs with a variety of dermatologic conditions, suggested a trend between severity of skin disease and the extent of Fc receptor secretion.
Fehrer
S L
SL
Department of Medical Science, College of Veterinary Medicine, University of Florida, Gainesville 32610.
Boyle
M D
MD
Halliwell
R E
RE
D000818:Animals; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology*; D006099:Granuloma / Q000382:microbiology / Q000662:veterinary*; D011711:Pyoderma / Q000382:microbiology / Q000662:veterinary*; D012867:Skin / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000032:analysis*; D013210:Staphylococcus / Q000302:isolation & purification*
D013205:Staphylococcal Protein A