Expression of a DNA-ligase-stimulatory factor in Bloom's syndrome cell line GM1492.
An increased DNA ligase activity is observed in extracts of Bloom's syndrome (BS) fibroblast cell line GM1492. The activity is 2-3-fold higher in this cell line compared to normal human fibroblasts, and 5-20-fold higher than in three other BS cell lines investigated. The DNA ligase activity in GM1492 cells is promoted by a heat-resistant, protease-sensitive factor comigrating with DNA ligases on single-stranded-DNA--cellulose. The factor stimulates DNA ligase I as well as DNA ligase II, and is not identical to the activity-promoting homologous DNA pairing, which is also enhanced in GM1492 cell extracts.
Kenne
K
K
Department of Medical Cell Genetics, Karolinska Institutet, Stockholm, Sweden.
Ljungquist
S
S
D000818:Animals; D001816:Bloom Syndrome / Q000201:enzymology*; D002417:Cattle; D002460:Cell Line; D011088:DNA Ligases / Q000235:genetics*; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000201:enzymology; D005786:Gene Expression Regulation; D006801:Humans; D011118:Polynucleotide Ligases / Q000235:genetics*; D013950:Thymus Gland / Q000201:enzymology
D011088:DNA Ligases; D011118:Polynucleotide Ligases