title: A colorimetric procedure to monitor the extent of crosslinking of proteins by bisimidoesters.
abstract: Reaction of proteins with bisimidoesters is one of the popular crosslinking techniques. The extent of the reaction cannot be followed directly using the commonly used free amino group-determining reagents ninhydrin and 2,4,6-trinitrobenzene sulfonic acid since they react with bisimidoesters. However, we found that the extent of amidination of protein could be followed colorimetrically by using 1-fluoro-2,4-dinitrobenzene.
authors.affiliation: Chemistry Department, Indian Institute of Technology, New Delhi.
authors.forename: A
authors.initials: A
authors.lastname: Kamra
authors.forename: M N
authors.initials: MN
authors.lastname: Gupta
mesh_terms: D000818:Animals; D002417:Cattle; D003124:Colorimetry / Q000379:methods*; D003432:Cross-Linking Reagents; D004139:Dinitrofluorobenzene; D009578:Nitrobenzenes; D011506:Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate
chemical_list: D003432:Cross-Linking Reagents; D009578:Nitrobenzenes; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D004139:Dinitrofluorobenzene