[Tumor and stromal cell counts in the lung clones of transplantable rhabdomyosarcoma of rats determined by flow cytometry].
The phase G1-tumour cells of 76 lung clones obtained from rat transplantable rhabdomyosarcoma had near triploid mean DNA content (the diploid index (DI) = 1.46-2.05) and the phase G1/o-stromal cells was characterized by the diploid DNA content. The percent of tumour cells in lung clones determined by flow cytometry varied from 19.7 to 70.9. A correlation is found between the following items: DI; coefficients of variability of DNA content in tumour and stromal cells; the share of the tumour cells in clones and the coefficient of variability of the DNA content in stromal cells; percentage of the tumour cells in the So+ (G2 + M) phase and percentage of the stromal cells etc. Positive correlation is revealed between the coefficient of variability of the DNA content in tumour cells and coefficients of variability of the DNA content in stromal cells (r = 0.83), the negative one (r = 0.38)--between the coefficient of variability of the DNA content of stromal cells of clones and percentage of tumour cells which produce artificial lung metastasis.
Fedorov
L Iu
LIu
Gol'tsova
T A
TA
Rozanov
Iu M
IuM
D000818:Animals; D002452:Cell Count / Q000379:methods; D002467:Cell Nucleus / Q000032:analysis; D002999:Clone Cells / Q000032:analysis / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D008175:Lung Neoplasms / Q000032:analysis / Q000473:pathology* / Q000556:secondary; D009368:Neoplasm Transplantation; D051381:Rats; D012208:Rhabdomyosarcoma / Q000032:analysis / Q000473:pathology*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm
Soderzhanie opukholevykh i stromal'nykh kletok v klonakh legkikh perevivnoĭ rabdomiosarkomy krys, vyiavliaemoe metodom protochnoĭ tsitometrii.