title: Radiation-induced recovery processes in cultured marsupial cells.
abstract: The ultraviolet sensitivity of Potorous tridactylus male kidney (PtK-2) cells is markedly increased by post irradiation treatment for 24 h with 5 microM emetine (which inhibits protein synthesis by acting on the 40S ribosomal subunit), or with 5 microM cycloheximide (which inhibits by interaction with the 60S subunit), or with the RNA polymerase II inhibitor 5,6-dichloro-1-beta-ribofuranosylbenzimidazole at 50 microM. All 3 treatments give the same sensitivity, while unirradiated cells are little affected. Shortening the time of treatment, or delaying application of the drugs decreases their effect on the same time schedule. Preiirradiation of cells, with no drug treatment in the following 8 h, diminishes the sensitivity to a subsequent irradiation with protein synthesis blocked afterwards. Photoreactivation immediately following such preirradiation eliminates its desensitizing effect. Inhibiting protein synthesis after irradiation also markedly reduces the frequency of UV-induced mutants in the surviving population. These facts suggest that gene expression in the period following irradiation facilitates recovery from radiation damage, with an increased probability of mutation, reminiscent of the "SOS response" in Escherichia coli.
authors.affiliation: Programs in Biology, University of Texas, Dallas 75083-0688.
authors.forename: R
authors.initials: R
authors.lastname: Overberg
authors.forename: G
authors.initials: G
authors.lastname: Chandrasena
authors.forename: C S
authors.initials: CS
authors.lastname: Rupert
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D003513:Cycloheximide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D004640:Emetine / Q000494:pharmacology; D007668:Kidney; D007700:Kinetics; D008297:Male; D008394:Marsupialia; D014466:Ultraviolet Rays
chemical_list: D003513:Cycloheximide; D004640:Emetine