title: Cell-cycle dependent transformation competence in Dictyostelium discoideum.
abstract: We describe a modification of the transformation procedure for Dictyostelium which allows for a more exact estimate of transformation efficiency and the isolation of primary transformants. Investigations of transformation competence revealed a negative correlation to cell density and a distinct distribution during the cell-cycle. In synchronized cells, transformation efficiency is 2-3 fold higher during mitosis when compared to unsynchronized cells.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Nellen
authors.forename: U
authors.initials: U
authors.lastname: Saur
mesh_terms: D002453:Cell Cycle; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D007700:Kinetics; D014170:Transformation, Genetic