31P NMR study of daunorubicin-d(CGTACG) complex in solution. Evidence of the intercalation sites.
The interaction of daunorubicin with the self-complementary DNA fragment d(CGTACG) was studied by 31P NMR spectroscopy. The individual phosphates have been assigned for the nucleotide and the complex and signals from bound and free species in slow exchange at 19 degrees C were detected. In solution, the hexanucleotide binds two molecules of daunorubicin, which intercalate in the d(CG) sequence at both ends of the helix. Evidence for local deformations of the backbone at the sites of C5pG6, C1pG2 and G2pT3 phosphates is given. The binding constants for the stepwise equilibrium and the rate of dissociation of the intercalated duplex were also determined.
Ragg
E
E
Istituto di Biochimica e Chimica, Università di Milano, Italy.
Mondelli
R
R
Battistini
C
C
Garbesi
A
A
Colonna
F P
FP
D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts; D003630:Daunorubicin / Q000378:metabolism*; D007364:Intercalating Agents / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D012996:Solutions
D018736:DNA Adducts; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D010710:Phosphates; D012996:Solutions; C020270:daunorubicin-DNA; D004247:DNA; D003630:Daunorubicin