[Trial elution of semen stains and measurement of IgG level: a contribution to Gm typing].
The conditions for the elution of IgG in seminal stains have been investigated systematically. The amount of IgG recovered could neither or hardly be influenced by variation of the time (15 minutes to 120 hours) and temperature (4 degrees C, 20 degrees C, 37 degrees C, 56 degrees C) of elution, nor by mechanical treatment (cutting in small pieces, crushing), ultrasonic treatment or addition of a detergent. For fresh traces and such of an age of several weeks an elution time of 30 minutes at room temperature is sufficient; for very old stains an elution up to 2 hours may be recommended for safety. The investigations were restricted to the measurement of the IgG concentration in the eluates. No statement can yet be given about the biological value of the IgG for Gm typing due to this investigation alone.
Scheithauer
R
R
Institut für Rechtsmedizin der Universität Erlangen-Nürnberg.
Eckert
I
I
D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000235:genetics; D007129:Immunoglobulin Gm Allotypes / Q000032:analysis*; D008297:Male; D012661:Semen / Q000032:analysis*; D013696:Temperature; D013997:Time Factors
D007074:Immunoglobulin G; D007129:Immunoglobulin Gm Allotypes
Elutionsversuche an Spermaflecken und Messung der IgG-Konzentration: Ein Beitrag zur Gm-Bestimmung.