Comparison of the inhibitory effects of some compounds present in crude oils on rat platelet aggregation: role of intra- and extra- cellular calcium.
In vitro addition of some representative aliphatic, aromatic or heterocyclic compounds present in petroleum crude oils to washed rat platelets resulted in a concentration-dependent inhibition of aggregation induced by ADP or thrombin. Increasing concentration of extracellular Ca2+ did not alter the pattern of inhibition. ADP-induced intracellular Ca2+ mobilization was unaffected by most of the compounds tested. However, Ca2+ uptake was significantly inhibited when platelets were preincubated with these agents. This suggests that some components of crude oil may inhibit platelet aggregation by bringing about alterations in the platelet plasma membrane.
Chaudhury
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S
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
Khan
S
S
Rahimtula
A D
AD
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D005648:Fuel Oils / Q000032:analysis / Q000633:toxicity*; D008297:Male; D010578:Petroleum / Q000633:toxicity*; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013917:Thrombin / Q000494:pharmacology
D005648:Fuel Oils; D010578:Petroleum; D000244:Adenosine Diphosphate; D013917:Thrombin; D002118:Calcium