Basal and hypothalamic extract-induced luteinizing hormone and prolactin secretion by cultured anterior pituitary cells from female turkeys in various stages of the reproductive cycle.
The wet weight of the anterior pituitary gland of the domestic turkey increases as the hen progresses through the reproductive cycle. The greatest wet weight was observed with anterior pituitary glands from incubating hens, whose weight was twice that of anterior pituitary glands from nonphotostimulated hens. Anterior pituitary glands from hens in each of the various stages of the reproductive cycle were dissociated and cultured, and subsequently basal and hypothalamic extract (HE)-induced releases of prolactin (PRL) and luteinizing hormone (LH) were compared between cultures containing a defined number of anterior pituitary cells. Basal and HE-induced releases of PRL were greatest (P less than 0.05) in the cultures of anterior pituitary cells derived from incubating hens, with those of laying, photorefractory, and nonphotostimulated hens being successively less (P less than 0.05), respectively. HE-induced release of LH was greatest (P less than 0.05) in cell cultures derived from nonphotostimulated hens, with cultures of cells derived from laying, photorefractory, and incubating hens exhibiting successively smaller releases of LH (P less than 0.05), respectively. The concentration of HE that induced the first significant increase in the release of PRL or LH above that of basal levels also varied with the reproductive stage of the donor hens. The changes in circulating PRL levels during the various stages of the reproductive cycle reflect changes in anterior pituitary sensitivity to hypothalamic releasing activity and/or changes in the PRL releasing capacity of the anterior pituitary. In contrast, there does not appear to be a strong correlation between anterior pituitary LH releasing capacity in vitro and circulating LH levels in the domestic turkey hen.
el Halawani
M E
ME
Department of Animal Science, University of Minnesota, St. Paul 55108.
Silsby
J L
JL
Fehrer
S C
SC
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006065:Gonadotropins, Pituitary / Q000378:metabolism*; D007031:Hypothalamus / Q000032:analysis*; D007986:Luteinizing Hormone / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism; D012098:Reproduction; D014020:Tissue Extracts / Q000494:pharmacology*; D014422:Turkeys / Q000502:physiology*
D006065:Gonadotropins, Pituitary; D014020:Tissue Extracts; D011388:Prolactin; D007986:Luteinizing Hormone