Red blood cells protect endothelial cells against H2O2-mediated but not hyperoxia-induced damage.
We studied the effect of intact red blood cells on the exogenous H2O2-mediated damage as well as on the hyperoxia-induced injury of cultured endothelial cells. Red blood cells protected endothelial cells against H2O2-mediated injury efficiently, but had no effect on the hyperoxia-induced damage. Failure of red blood cells to protect endothelial cells against hyperoxia-induced injury was not due to hemolysis. Furthermore, hyperoxia-exposed red blood cells were still capable of protecting endothelial cells against H2O2-mediated damage.
Tsan
M F
MF
Research Service, Veterans Administration Medical Center, Albany, New York.
White
J E
JE
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002860:Chromium Radioisotopes / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010100:Oxygen / Q000494:pharmacology*
D002860:Chromium Radioisotopes; D006861:Hydrogen Peroxide; D010100:Oxygen