Evaluation of an enzymatic method for determining creatinine in plasma.
An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages over Jaffé-based methods--namely, smaller sample size, rapid sample throughput (200 per hour), and improved specificity. Glucose, acetoacetate, and cefoxitin did not interfere with the enzymatic method, although bilirubin did cause a negative interference which depended on both creatinine and bilirubin concentrations. The enzymatic method has particular clinical application in neonates, diabetic ketotic patients, and those receiving cephalosporins.
Crocker
H
H
Department of Biochemistry and Chemical Pathology, Flinders Medical Centre, Bedford Park, South Australia.
Shephard
M D
MD
White
G H
GH
D000090:Acetoacetates; D000293:Adolescent; D001663:Bilirubin; D002440:Cefoxitin; D003124:Colorimetry; D003404:Creatinine / Q000097:blood*; D005260:Female; D005947:Glucose; D006801:Humans; D007223:Infant; D008722:Methods; D011933:Reagent Kits, Diagnostic
D000090:Acetoacetates; D011933:Reagent Kits, Diagnostic; D002440:Cefoxitin; D003404:Creatinine; D005947:Glucose; D001663:Bilirubin