Stimulation of heart sarcolemmal Na+-Ca2+ exchange by concanavalin A.
The effects of Concanavalin A (Con A) on membrane Ca2+ translocation activities were examined by employing rat heart sarcolemmal preparations. Con A stimulated Na+-dependent Ca2+ uptake and ATP-dependent Ca2+ uptake activities in the sarcolemmal vesicles; maximal stimulation was seen at a concentration of 10 microgram/ml. These effects of Con A were blocked by alpha-metylmannoside. Sarcolemmal Na+-induced Ca2+ release was not affected by Con A. It is suggested that Con A-like substances may play a regulatory role in Ca2+-translocation processes of heart sarcolemma.
Makino
N
N
Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, Canada.
Zhao
D
D
Dhalla
N S
NS
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology*; D006352:Heart Ventricles / Q000378:metabolism; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D019831:Sodium-Calcium Exchanger
D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D003208:Concanavalin A; D012964:Sodium; D002118:Calcium