The effect of elevated temperature on the aggregation of African green monkey kidney cells.
The aggregation kinetics of African green monkey kidney cells CV1 and of the SV40 transformed derivative COS1 cells that had been incubated at 37 degrees C or 43.5 degrees C was studied using the shaking flask system. COS1 cells show a three fold decrease in aggregation rate compared to CV1 cells when both cell types were incubated and aggregated at 37 degrees C. When these cell types were incubated at 43.5 degrees C for 5 hours, then aggregated at 37 degrees C showed a faster aggregation kinetics than before. Their aggregation at 43.5 degrees C with prior incubation at 37 degrees C or 43.5 degrees C reached the aggregation kinetics of 43.5 degrees C incubated cells aggregated at 37 degrees C. The addition of serum in the aggregation medium did not influence extensively the aggregation rates of both cell types.
Matthopoulos
D P
DP
Laboratory of General Biology, School of Medicine, University of Ioannina, Greece.
Tzaphlidou
M
M
D000818:Animals; D001769:Blood; D001831:Body Temperature; D002449:Cell Aggregation; D002460:Cell Line; D002461:Cell Line, Transformed; D002522:Chlorocebus aethiops; D003470:Culture Media; D007668:Kidney / Q000166:cytology*; D007700:Kinetics
D003470:Culture Media