Measurement of human corticosteroid binding globulin by enzyme-linked immunoassay.
Thirteen monoclonal antibodies have been raised against corticosteroid binding globulin (CBG). From four of those with highest affinity for the antigen, two were selected for development of a sandwich enzyme-linked immunoassay (ELISA). The sensitivity of the assay was such that 0.7 fmol CBG could be detected. Levels of the binding protein in men (740 +/- 67 nmol/l) and women (690 +/- 103 nmol/l) were not significantly different, while those found during the third trimester of pregnancy (1500 +/- 423 nmol/l) were approximately twice these levels. CBG denatured by heating to 60 degrees C could not be detected by the ELISA.
Fantl
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Imperial Cancer Research Fund, Lincoln's Inn Fields, London, England.
Wang
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Mockridge
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CI
D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D006854:Hydrocortisone / Q000378:metabolism; D007114:Immunization; D007457:Iodine Radioisotopes; D008297:Male; D011247:Pregnancy; D011489:Protein Denaturation; D012016:Reference Values; D014156:Transcortin / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D014156:Transcortin; D006854:Hydrocortisone