A combined method of acetylcholinesterase histochemistry and [3H]thymidine autoradiography: application to neurogenesis of the rat basal forebrain cholinergic system.
Rats labelled with [3H]thymidine on embryonic day 15 were treated postnatally with diisopropylfluorophosphate and 2-3 h later perfused with 10% buffered formalin. Cryostat sections of the basal forebrain were autoradiographed and subsequently stained for acetylcholinesterase (AChE). It was shown that this procedure did not significantly influence either the AChE activity or the silver grains over [3H]thymidine-labelled nerve cell nuclei. The labelling index evaluated in cholinergic forebrain regions (medial septum, diagonal band of Broca, substantia innominata, nucleus basalis of Meynert) indicated a caudorostral gradient of the formation of AChE-containing neurons while formation of non-cholinergic neurons showed a more even pattern.
Schwab
C
C
Paul Flechsig Institute of Brain Research, Department of Neurochemistry, Karl Marx University, Leipzig, G.D.R.
Brückner
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G
Mares
V
V
Biesold
D
D
D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001479:Basal Ganglia / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D002454:Cell Differentiation; D002799:Cholinergic Fibers / Q000166:cytology / Q000201:enzymology*; D006651:Histocytochemistry / Q000379:methods*; D051381:Rats; D013936:Thymidine
D000110:Acetylcholinesterase; D013936:Thymidine