Colorimetric detection of the hydroxyl radical: comparison of the hydroxyl-radical-generating ability of various iron complexes.
An aromatic substrate for hydroxylation by OH radicals has been synthesized. Neither the substrate nor its hydroxylated product binds either iron(III) or iron(II). A spectrophotometric assay based on the hydroxylation of this substrate, N,N'-(5-nitro-1,3-phenylene)bisglutaramide, is described.
Singh
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Department of Chemistry, University of Essex, Colchester, United Kingdom.
Hider
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D003124:Colorimetry; D005977:Glutarates; D006900:Hydroxylation; D007502:Iron Chelating Agents / Q000032:analysis*; D013329:Structure-Activity Relationship
D005977:Glutarates; D007502:Iron Chelating Agents; C056819:N,N'-(5-nitro-1,3-phenylene)bisglutaramide