Hydrolysis of low-density lipoprotein phospholipids in arterial smooth muscle cells.
The hydrolysis of phosphatidylcholine (PC) associated with low-density lipoprotein (LDL) by homogenates of smooth muscle cells from rabbit aorta was studied. 1-Palmitoyl-2-[14C]oleoylPC associated with LDL (LDL-P[14C]OPC) or 1-linoleoyl-2-[14C]linoleoylPC associated with LDL (LDL-L[14C]LPC) was used as the substrate. The optimum pH for the formation of [14C]oleoyllysoPC from LDL-P[14C]OPC and for the formation of [14C]linoleoyllysoPC from LDL-L[14C]LPC was pH 4.5, and pH 4.5 and 7.0, respectively. These activities were designated as phospholipase A1 activities. The optimum pH values for the formation of [14C]oleate from LDL-L[14C]OPC and for the formation of [14C]linoleate from LDL-L[14C]LPC were pH 4.5 and 6.5, and pH 4.5, 6.5 and 8.5, respectively. These activities were designated as phospholipase A2 activities. Ca2+ did not affect acid phospholipase A1 activity, but decreased acid phospholipase A2 activity for the hydrolysis of LDL-L[14C]LPC. When smooth muscle cells were incubated with LDL, both phospholipase A1 and phospholipase A2 activities at pH 4.5 for the hydrolysis of LDL-L[14C]LPC increased significantly. These results indicate that phospholipases A1 and A2, which hydrolyze PC associated with LDL, exist in arterial smooth muscle cells and are involved in the metabolism of LDL incorporated into these cells.
Ishikawa
Y
Y
Second Department of Internal Medicine, Chiba University School of Medicine, Japan.
Nishide
T
T
Sasaki
N
N
Shirai
K
K
Saito
Y
Y
Yoshida
S
S
D000818:Animals; D001158:Arteries; D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D010741:Phospholipases A / Q000032:analysis / Q000502:physiology; D054466:Phospholipases A1; D054467:Phospholipases A2; D011817:Rabbits
D008077:Lipoproteins, LDL; D010713:Phosphatidylcholines; D010741:Phospholipases A; D054466:Phospholipases A1; D054467:Phospholipases A2