Detection and characterization of human serum antibodies to polycyclic aromatic hydrocarbon diol-epoxide DNA adducts.
The presence of serum antibodies to the diol-epoxide DNA adducts of representative polycyclic aromatic hydrocarbons (PAH), chrysene, benz[a]anthracene and benzo[a]pyrene, was determined by ELISA using serum samples obtained from normal healthy individuals. Antibodies that reacted against PAH adducted-DNA, but not against PAH-adducted protein, were found in the serum of approximately 40% of the test individuals. Specificity analysis of the antibodies demonstrated that serological cross-reactions between the benzo[a]pyrene and the chrysene diol-epoxide adducts were present. Similar cross-reactivity between the benz[a]anthracene and the chrysene adducts was observed. Sera containing antibodies that were apparently specific for each of the three PAH-DNA adducts were also identified. The presence of antibodies to PAH-DNA adducts indicates both past exposure to these carcinogenic PAH and their metabolic activation to the DNA damaging metabolites. These antibodies may prove to be useful in both retrospective and prospective epidemiological studies of various diseases associated with PAH exposure.
Newman
M J
MJ
Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
Light
B A
BA
Weston
A
A
Tollurud
D
D
Clark
J L
JL
Mann
D L
DL
Blackmon
J P
JP
Harris
C C
CC
D000328:Adult; D000367:Age Factors; D000368:Aged; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001551:Benz(a)Anthracenes / Q000276:immunology; D001564:Benzo(a)pyrene; D001580:Benzopyrenes / Q000276:immunology; D001666:Binding Sites, Antibody; D002274:Carcinogens, Environmental; D002911:Chrysenes / Q000276:immunology; D004247:DNA / Q000276:immunology; D018736:DNA Adducts; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011083:Polycyclic Compounds / Q000276:immunology*; D012737:Sex Factors; D012907:Smoking / Q000276:immunology
D000906:Antibodies; D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D002274:Carcinogens, Environmental; D002911:Chrysenes; D018736:DNA Adducts; D011083:Polycyclic Compounds; C056615:benz(a)anthracene-DNA adduct; C056612:benzo(a)pyrene-DNA adduct; C056613:chrysene-DNA adduct; D001564:Benzo(a)pyrene; D004247:DNA