Effects of cholecystokinin and hydrocortisone on DNA and protein synthesis in immature rat pancreas.
To define the developmental pattern of the trophic effects of cholecystokinin octapeptide (CCK-8) and hydrocortisone on immature rat pancreas, we injected newborn rats, rats aged 4, 7, 11, 18, and 25 days and 3 months, and adult rats with CCK (5 and 10 micrograms/kg) in gelatin and hydrocortisone (10 mg/kg) for 3 days. Animals were killed, the pancreata were removed, and the concentrations of DNA and protein were measured and DNA and protein synthesis rates determined by incorporation of [3H]thymidine and [14C]leucine, respectively. These values were compared with those of saline-injected controls. DNA concentration was significantly increased over control at ages 2 days to adult by hydrocortisone and by CCK (10 micrograms/kg) in the adult. Protein concentration was increased on days 3-14 by hydrocortisone. DNA synthesis was increased by CCK and decreased by hydrocortisone at 3 months and adult. Protein synthesis was decreased by hydrocortisone at ages 3-14 days. Thus, each agent has its own developmental pattern with age on the rat pancreas.
Werlin
S L
SL
Department of Pediatrics, Medical College of Wisconsin, Milwaukee.
Virojanavat
S
S
Reynolds
E
E
Hoffman
R G
RG
Colton
D G
DG
D000367:Age Factors; D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D006854:Hydrocortisone / Q000494:pharmacology*; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D004247:DNA; D002766:Cholecystokinin; D006854:Hydrocortisone