title: The dioxin receptor: characterization of its DNA-binding properties.
abstract: The binding of the rat hepatic dioxin and glucocorticoid receptors to the polyanionic matrices heparin-Sepharose and DNA-cellulose in vitro and to cell nuclei in vivo was studied under various conditions. In a non-liganded and non-activated state both receptors eluted from heparin-Sepharose at a low ionic strength and were not retained on DNA-cellulose. Following ligandation and activation in vitro both receptors showed an increased affinity for heparin-Sepharose and were retained on DNA-cellulose. In analogy to these in vitro data, it was found that a high salt concentration (0.4 M KCl) was required to extract in vivo liganded dioxin receptor from purified nuclear preparations in contrast to that previously reported for non-liganded nuclear receptors. Limited proteolysis of both dioxin and glucocorticoid receptors resulted in molecular species of similar binding properties with regard to DNA-cellulose and heparin-Sepharose. We conclude that, in addition to the dioxin and glucocorticoid receptors showing considerable similarities in their physicochemical properties, they may also share a similar structural organization with regard to functional domains.
authors.affiliation: Department of Medical Nutrition, Karolinska Institutet, Huddinge University Hospital, Sweden.
authors.forename: G G
authors.initials: GG
authors.lastname: Mason
authors.forename: A
authors.initials: A
authors.lastname: Wilhelmsson
authors.forename: S
authors.initials: S
authors.lastname: Cuthill
authors.forename: M
authors.initials: M
authors.lastname: Gillner
authors.forename: L
authors.initials: L
authors.lastname: Poellinger
authors.forename: J A
authors.initials: JA
authors.lastname: Gustafsson
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism*; D004147:Dioxins / Q000378:metabolism*; D008024:Ligands; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats; D011955:Receptors, Drug / Q000302:isolation & purification / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D004147:Dioxins; D008024:Ligands; D011955:Receptors, Drug; D011965:Receptors, Glucocorticoid; D004247:DNA