Culture conditions for arresting and stimulating the proliferation of a rainbow trout fibroblast cell line, RTG-2.
Conditions for arresting and stimulating the proliferation of the rainbow trout fibroblast cell line RTG-2 have been examined and the time course of events after stimulation determined. Quiescent populations were achieved in two ways. Cultures grown to confluency without a medium change for at least 7 d had fewer than 5% of the cells in S phase and few mitotic figures. Cultures deprived of serum, which could be done for up to 3 d without a loss in cell number, also achieved quiescence. After 3 d without serum, less than 1% of cells were in S phase and mitotic figures were infrequent. Addition to these cultures of fresh serum-containing medium brought about the synchronous entry of cells into S phase and mitosis. For cultures in which either the medium had been changed after 7 d without a change or serum-containing medium had been added after 3 d of serum deprivation, DNA synthesis increased after a lag period of 20 to 24 h, was pronounced between 30 and 45 h, and then declined. This was followed by a peak in the mitotic index. These protocols for arresting and subsequently stimulating RTG-2 proliferation should allow the G1-S transition to be studied in a representative of teleosts.
Lee
L E
LE
Department of Biology, University of Waterloo, Ontario, Canada.
Martinez
A
A
Bols
N C
NC
D000818:Animals; D002455:Cell Division; D005347:Fibroblasts / Q000166:cytology*; D006133:Growth Substances / Q000494:pharmacology; D012487:Salmonidae / Q000033:anatomy & histology*; D013997:Time Factors; D014337:Trout / Q000033:anatomy & histology*
D006133:Growth Substances