[Relation of the biological activity of double helical interferon inducers and their penetration into the cell and suppression of protein synthesis].
The quantities of 125I-ds-inductors of interferon penetrating into the cells of transplantable cultures such as M19 (human fibroblast cells) and L-929 (mouse line) were not significant i.e. 10.5-4 per cent of the drug added. Under conditions of transfection with calcium phosphate and in complex with DEAE dextran the quantities of the inductors adhering to the cells and their contents in the cytoplasmic and nuclear fractions markedly increased. During the transfection with calcium phosphate up to 50 per cent of the applied inductor bound to the cells and its content in the cytoplasm and nuclei reached at least 10 per cent. After penetration into the cells poly I.poly C probably maintained its native structure and appeared to be firmly bound to the nuclear material. Preliminarily hydrolyzed inductors showed no such penetrating capacity. Contrary to the human fibroblast cells, in the mouse cells L-929 treated with the ds-inductors there was observed inhibition of the total protein synthesis which was probably due to activation of enzymes such as 2-5A-synthetase and proteinkinase. Increased penetration of the ds-inductors into the cells was accompanied by a marked (from 10- to 1000-fold) rise in their antiviral activity and a 2-4-fold rise in their interferon-inducing activity. It was concluded that there was immediate dependence of ds-inductor biological activity manifestation on the level of the inductor penetration into the cells.
Radomskaia
N A
NA
Sokolova
T M
TM
Ershov
F I
FI
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D005347:Fibroblasts; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D007739:L Cells; D051379:Mice; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D014162:Transfection
D000998:Antiviral Agents; D007369:Interferon Inducers; D011500:Protein Synthesis Inhibitors
Sviaz' biologicheskoĭ aktivnosti dvuspiral'nykh induktorov interferona s proniknoveniem v kletki i podavleniem sinteza belkov.