A rapid and sensitive method for the determination of gamma-hydroxybutyric acid and trans-gamma-hydroxycrotonic acid in rat brain tissue by gas chromatography/mass spectrometry with negative ion detection.
gamma-Hydroxybutyric acid (GHB) and trans-gamma-hydroxycrotonic acid (HCA) together with their respective internal standards were derivatized to give the pentafluorobenzyl esters of the N-tert-butyldimethylsilyl derivatives. These compounds give, under electron capture conditions, very simple negative ion mass spectra. A very sensitive and specific assay for GHB and HCA in brain tissue (detection limit of about 5 pg per injection) using gas chromatography/negative ion mass spectrometry is described. The average levels measured in the whole brain were 1.10 +/- 0.18 nmol GHB/g wet weight and 0.18 +/- 0.02 nmol HCA/g wet weight.
Ehrhardt
J D
JD
Spectrometrie de Masse, Institut de Pharmacologie (UA 589 CNRS), Strasbourg, France.
Vayer
P
P
Maitre
M
M
D000818:Animals; D001923:Brain Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006885:Hydroxybutyrates / Q000032:analysis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012978:Sodium Oxybate / Q000032:analysis*
D006885:Hydroxybutyrates; C035142:4-hydroxy-2-butenoic acid; D012978:Sodium Oxybate