Influence of the patch pipette glass on single acetylcholine channels recorded from Xenopus myocytes.
The type of glass used to fabricate patch pipettes influences the kinetic properties of single-channel currents activated by acetylcholine in Xenopus myocytes. In patches formed with hard glass pipettes, the opening rate decreased in a time-dependent manner. Such decrease was most pronounced for the low-conductance channel. In addition, longer-duration events of this form of channel dropped out faster than shorter-duration events and, as a consequence, the apparent channel open time became shorter during the recording period. These effects were not observed in patches formed with soft glass pipettes.
Rojas
L
L
Institute of Neurobiology, University of Puerto Rico Medical Sciences Campus, San Juan 00901.
Zuazaga
C
C
D000818:Animals; D002478:Cells, Cultured; D004553:Electric Conductivity; D004625:Embryo, Nonmammalian; D005898:Glass; D009132:Muscles / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000502:physiology*; D014982:Xenopus laevis
D011950:Receptors, Cholinergic