title: Interactions of high density lipoprotein subclasses (HDL2 and HDLc) with dog adipocytes: selective effects of cholesterol and saturated fat feeding.
abstract: Adipose tissue is a cholesterol storage organ and derives its cholesterol primarily from circulating lipoproteins. The present study shows that adipocytes isolated from canine omental fat tissue interact specifically with high density lipoprotein subfractions lacking or enriched in apolipoprotein E, namely canine high density lipoprotein-2 (HDL2) and HDLc, respectively. While 125I-labeled HDL2 binding was inhibited similarly by both excess unlabeled HDLc and HDL2, 125I-labeled HDLc interaction was inhibited by its homologous ligand only. Paired studies showed that the amount of HDLc associated with adipocytes was significantly higher compared to HDL2. The effect of a short-term cholesterol and saturated fat feeding on adipocyte-HDL interaction was examined using fat cells obtained from dogs before and again 3 weeks after a diet supplemented with cholesterol (1% w/w) and saturated fat (30% lard, w/w). Significant increases in body weight and omental fat cell weight occurred after fat feeding. The amount of 125I-labeled HDL2 that could be bound to adipocytes increased after the diet, whether expressed on a per cell basis (P less than 0.005) or per unit cell surface (P less than 0.025). The amount of cell-associated 125I-labeled HDLc, however, was not significantly affected by the cholesterol-rich diet. The characteristics of HDLc and HDL2 dissociation were assessed by examining the release of labeled lipoproteins from adipocytes preincubated with 125I-labeled HDLc and 125I-labeled HDL2. HDL2 dissociation from adipocytes was significantly decreased (P less than 0.05) following the diet and may explain in part the apparent increase in cell-associated 125I-labeled HDL2.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, University of Toronto, Ontario, Canada.
authors.forename: B S
authors.initials: BS
authors.lastname: Fong
authors.forename: J P
authors.initials: JP
authors.lastname: Despres
authors.forename: P
authors.initials: P
authors.lastname: Julien
authors.forename: A
authors.initials: A
authors.lastname: Angel
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D008076:Cholesterol, HDL / Q000378:metabolism*; D004041:Dietary Fats / Q000494:pharmacology*; D004285:Dogs; D066298:In Vitro Techniques; D008075:Lipoproteins, HDL / Q000378:metabolism*; D053439:Lipoproteins, HDL2
chemical_list: D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D004041:Dietary Fats; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2; D002784:Cholesterol