title: [Physico-chemical and functional characteristics of a subfraction of low-density lipoproteins isolated by ion-exchange column chromatography].
abstract: Fractionation of human blood plasma low density lipoproteins (LDL) was performed by ion-exchange chromatography, using a linear NaCl gradient. It was shown that the binding of LDL subfractions eluted with a low ionic strength buffer (i.e., containing the particles with a lower negative charge) to B, E-receptors of fibroblasts was more effective than that of subfractions eluted with a high ionic strength buffer (i.e., containing the particles with a higher negative charge). The LDL particles with a lower negative charge had lower values of flotation coefficients (according to analytical ultracentrifugation data), smaller dimensions (according to gradient gel electrophoresis data) and a lower phospholipid/protein ratio (w/w). The experimental results suggest that LDL subfractions having different electrical parameters of the particle surface also differ in other physicochemical properties and seem to play a different role in atherogenesis.
authors.forename: I N
authors.initials: IN
authors.lastname: Gorshkova
authors.forename: N V
authors.initials: NV
authors.lastname: Medvedeva
authors.forename: A D
authors.initials: AD
authors.lastname: Morozkin
authors.forename: I V
authors.initials: IV
authors.lastname: Fuki
authors.forename: N N
authors.initials: NN
authors.lastname: Gorchakova
mesh_terms: D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification*; D011973:Receptors, LDL / Q000378:metabolism; D014461:Ultracentrifugation
chemical_list: D008077:Lipoproteins, LDL; D011973:Receptors, LDL
vernacular_title: Fiziko-khimicheskie i funktsional'nye kharakteristiki podfraktsii lipoproteidov nizkoĭ plotnosti, poluchennykh ionoobmennoĭ kolonochnoĭ khromatografieĭ.