Evidence that a glycolipid tail anchors antigen 117 to the plasma membrane of Dictyostelium discoideum cells.
We describe the biochemical features of the putative cell cohesion molecule antigen 117, indicating that it is anchored to the plasma membrane by a glycolipid tail. Antigen 117 can be radiolabeled with [3H]myristate, [3H]palmitate, and [14C]ethanolamine. The fatty acid label is removed by periodate oxidation and nitrous acid deamination, indicating that the fatty acid is attached to the protein by a structure containing carbohydrate and an unsubstituted glucosamine. As cells develop aggregation competence, the antigen is released from the cell surface in a soluble form that can still be radiolabeled with [14C]ethanolamine but not with [3H]myristate or [3H]palmitate. The molecular weight of the released antigen is similar to that found in the plasma membrane, but it preferentially partitions in Triton X-114 as a hydrophilic, as opposed to a hydrophobic, protein. Plasma membranes contain the enzyme activity responsible for the release of the antigen in a soluble form.
Sadeghi
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E.A. Doisy Department of Biochemistry, St. Louis University School of Medicine, MO 63104.
da Silva
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AM
Klein
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D000954:Antigens, Surface / Q000494:pharmacology* / Q000633:toxicity; D001345:Autoradiography; D015815:Cell Adhesion Molecules; D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism; D004023:Dictyostelium / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D006017:Glycolipids / Q000378:metabolism*; D007118:Immunoassay; D008562:Membrane Glycoproteins / Q000378:metabolism*; D015800:Protozoan Proteins
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D006017:Glycolipids; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; C052291:cell cohesion molecule, Dictyostelium