[Cytogenetic and virological characteristics of the initial line and clones of RH cells with various degrees of sensitivity to the tick-borne encephalitis virus].
Virological and cytogenetic characterization of line RH and its two clones (RHk-20 and RHk-13/6), having different sensitivity to the tick-borne encephalitis virus (TBE), has been made. The RHk-13/6-cells are 10,000 times more sensitive to cytopathic effect of the tick-borne encephalitis virus in comparison with the RHk-20-cells. By its sensitivity to the virus the base line is in the intermediate position. Cytogenetic analysis revealed the variability interval decrease as to the chromosome number at cloning, and showed the structural stability of the line and clone karyotypes. The line RH and its clone K-20 have modal number of chromosomes equal to 65; the cell population with the chromosome number equal to 66 dominated in RHk-13/6. The application of methods of chromosome differential (G, C) staining and these for the detection of active nucleolar organizers (NO) permitted identifying the marker chromosomes and determining the difference between the clones and the line as associated with the presence of an additional homologue of chromosome 8 in the sensitive clone.
Popova
N A
NA
Mamaeva
S E
SE
Arkina
M A
MA
Miriutova
T L
TL
Laptakova
L M
LM
D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002999:Clone Cells / Q000382:microbiology / Q000648:ultrastructure; D003588:Cytopathogenic Effect, Viral; D004669:Encephalitis Viruses, Tick-Borne / Q000472:pathogenicity*; D006801:Humans; D007621:Karyotyping; D007668:Kidney / Q000382:microbiology / Q000648:ultrastructure*; D008677:Metaphase
Tsitogeneticheskaia i virusologicheskaia kharakteristika iskhodnoĭ linii i klonov kletok RH s razlichnoĭ chuvstvitel'nost'iu k virusu kleshchevogo éntsefalita.