Rapid detection (4 h) of methicillin-resistant Staphylococcus aureus by a bioluminescence method.
A 4-h bioluminescence method for methicillin susceptibility determination was compared with reference methods. Of the Staphylococcus aureus strains tested, 80 were methicillin resistant, 180 were methicillin susceptible, and 10 were borderline susceptible. There was 100% correlation between bioluminescence and reference methods for methicillin-susceptible and methicillin-resistant strains. All borderline-susceptible strains were identified as methicillin resistant by bioluminescence.
Park
C H
CH
Microbiology Laboratory, Fairfax Hospital, Falls Church, Virginia 22046.
Hixon
D L
DL
McLaughlin
C M
CM
Cook
J F
JF
D000255:Adenosine Triphosphate / Q000032:analysis*; D008163:Luminescent Measurements; D008712:Methicillin / Q000494:pharmacology; D010403:Penicillin Resistance; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D013997:Time Factors
D000255:Adenosine Triphosphate; D008712:Methicillin