[The potential dependence of acetylcholine-activated membrane conductance in sympathetic ganglion neurons].
Membrane conductance activated by acetylcholine (ACh-conductance) was studied in rat isolated superior cervical ganglion neurons by means of the patch-clamp method in the whole-cell recording mode. It was found that ACh-conductance was increased or decreased with membrane hyper- or depolarization, respectively. The decrease in ACh-conductance was not associated with the reversal of ACh-current or with the presence of Ca2+ ions in external solution. The time constant of voltage-jump relaxation of ACh-current revealed e-fold increase with membrane hyperpolarization by 70 mV, which corresponded to the voltage dependence of ACh-conductance. Basing upon these results it was concluded that the voltage dependence of ACh-conductance is mostly determined by the voltage dependence of nicotinic receptor channel gating kinetics.
Selianko
A A
AA
Derkach
V A
VA
Kurennyĭ
D E
DE
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D004553:Electric Conductivity; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D007478:Iontophoresis / Q000379:methods; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D013997:Time Factors
D000109:Acetylcholine
Potentsialozavisimost' aktiviruemoĭ atsetilkholinom provodimosti membran v neĭronakh simpaticheskogo gangliia.