Automated direct assay system for the measurement of sex steroid hormones in serum using high-performance liquid chromatography.
An automated direct assay system using high-performance liquid chromatography was developed for the simultaneous measurement of estradiol, estrone, progesterone, 17 alpha-hydroxyprogesterone, 20 alpha-hydroxyprogesterone, testosterone and androstenedione in biological fluids. A comparison between the values measured by this method and by radioimmunoassay revealed good correlation for estradiol (r = 0.938, p less than 0.001) and progesterone (r = 0.903, p less than 0.001). Estradiol and estrone could be analysed above the level of 250 pg/ml, and progesterone, 17 alpha-hydroxyprogesterone, 20 alpha-hydroxyprogesterone, testosterone and androstenedione could be analysed above the level of 5.0-7.5 ng/ml. The method was applied to the clinical appraisal of placental function and maturation of ovarian follicles.
Suzuki
Y
Y
Department of Obstetrics and Gynaecology, Okayama University Medical School, Japan.
Hayashi
N
N
Sekiba
K
K
D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D011247:Pregnancy; D011863:Radioimmunoassay
D012739:Gonadal Steroid Hormones; D007202:Indicators and Reagents