title: Crystallization of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10.
abstract: Haloalkane dehalogenases are enzymes that release chloride or bromide from n-halogenated alkanes. X-ray quality crystals of haloalkane dehalogenase from the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 have been grown at room temperature from 64% saturated ammonium sulfate solutions (pH 6.2 to 6.4). The crystals diffract in the X-ray beam to at least 2.4 A resolution (1 A = 0.1 nm). Their space group is P2(1)2(1)2, with cell dimensions a = 94.1 A, b = 72.8 A, c = 41.4 A and alpha = beta = gamma = 90 degrees. There is one monomer (molecular weight 36,000) per asymmetric unit.
authors.affiliation: Laboratory of Chemical Physics, University of Groningen, The Netherlands.
authors.forename: H J
authors.initials: HJ
authors.lastname: Rozeboom
authors.forename: J
authors.initials: J
authors.lastname: Kingma
authors.forename: D B
authors.initials: DB
authors.lastname: Janssen
authors.forename: B W
authors.initials: BW
authors.lastname: Dijkstra
mesh_terms: D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology*; D006867:Hydrolases; D008969:Molecular Sequence Data; D014961:X-Ray Diffraction
chemical_list: D006867:Hydrolases; C046137:haloalkane dehalogenase