title: Relationship between the high-energy phosphate content and various left ventricular functional parameters of the normal and hypertrophied heart after global ischemia and reperfusion.
abstract: Using an isolated rat heart preparation (Langendorff perfusion, perfusion pressure 100 cm H2O) the correlation between the high-energy phosphate content and various left ventricular (lv) functional parameters of the hypertrophied heart (spontaneous hypertensive rats lv/body weight ratio 3.6 +/- 0.5 x 10(-3) was determined after normo- (30 min) and hypothermic (25 degrees C, 120 min) cardioplegic arrest and reperfusion, and compared with normal hearts (Wistar rats lv/body weight ratio 2.0 +/- 0.3 x 10(-3). St. Thomas Hospital solution was used as the cardioplegic agent. Before ischemia hypertrophied hearts had a significantly higher developed left ventricular pressure, pressure rate product and dp/dtmax, but a significantly lower ATP and total adenine nucleotide content. Irrespective of the mode and temperature of cardiac arrest there was a strong correlation both for normal and for hypertrophied hearts between the high-energy phosphate content expressed as ATP, total adenine nucleotides or the "energy charge" and the left ventricular functional parameters pressure rate product and dp/dtmax. The correlation coefficient ranged from 0.80 to 0.89 and was highest when the ATP content was plotted against pressure rate product (r = 0.89). There was a different slope for normal and hypertrophied hearts with a steeper decline of the left ventricular function in hypertrophied hearts for any given reduction of the myocardial adenine nucleotide content. Our results indicate that a similar reduction of the ATP or total adenine nucleotide content in both the normal and hypertrophied heart reduces left ventricular function to a greater degree in the hypertrophied heart.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, University of Tübingen, FRG.
authors.forename: G
authors.initials: G
authors.lastname: Fenchel
authors.forename: R
authors.initials: R
authors.lastname: Storf
authors.forename: J
authors.initials: J
authors.lastname: Michel
authors.forename: H E
authors.initials: HE
authors.lastname: Hoffmeister
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002312:Cardiomyopathy, Hypertrophic / Q000473:pathology*; D003326:Coronary Circulation; D004734:Energy Metabolism; D006324:Heart Arrest, Induced; D009200:Myocardial Contraction; D009206:Myocardium / Q000473:pathology; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate