[Enzymatic activity of xenobiotic metabolism in rats with a varying body allowance of vitamin B1].
Vitamin B1 in rats induced in the liver elevation of activity of metabolic enzymes of xenobiotics bound with membranes (dimethylaniline N-demethylase, aniline n-hydroxylase, aryl esterase). At the same time activity of the cytoplasmatic enzymes alcohol dehydrogenase and glutathione-S-transferase was appreciably lowered. An additional load with thiamine (20 mg/kg) led to a drop in activity of the membrane-bound enzymes. Vitamin B1 deficiency modified the effect of the inductor phenobarbital. Additional administration of vitamin B1 to thiamine-deficient animals normalized the thiamine level in the liver, and activity of hydroxylase, aryl esterase, formaldehyde dehydrogenase, and significantly decreased demethylase activity. In vitamin B1-deficient animals high detergent concentrations significantly suppressed NADH-dichlorophenol-indophenol-reductase activity, while low concentrations activated this enzyme as compared to the control.
Lychko
A P
AP
Bogdanov
N G
NG
Pentiuk
A A
AA
Lutsiuk
N B
NB
D000818:Animals; D003902:Detergents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013831:Thiamine / Q000008:administration & dosage*; D013832:Thiamine Deficiency / Q000201:enzymology
D003902:Detergents; D013831:Thiamine; D010634:Phenobarbital
Aktivnost' fermentov metabolizma ksenobiotikov pri razlichnoĭ obespechennosti organizma krys vitaminom B1.