title: Cyst fluid of glioma does not inhibit the killing action of lymphokine-activated killer (LAK) cells in vitro.
abstract: In theory, lymphokine-activated killer (LAK) cells offer a potential method to treat cerebral gliomas, especially low-grade gliomas. LAK cells would be administered by repeated injections straight into the cavity of a subtotally removed tumour. However, brain-tumour cyst fluid has been shown to be immunosuppressive in lymphocyte stimulation tests. Therefore we wanted to know whether the fluid would reduce the killing efficacy of LAK cells. Using a standard cytotoxity test based on 51Cr release, we compared in vitro the cytotoxity of LAK cells against K-562 tumour cells in brain-tumour cyst fluid, autologous serum and allogeneic serum. Five patients with cystic glioma and one with cystic meningioma were studied and no inhibition of cytotoxity of LAK cells was observed.
authors.affiliation: Department of Neurosurgery, Helsinki University Central Hospital, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Jääskeläinen
authors.forename: P
authors.initials: P
authors.lastname: Kalliomäki
authors.forename: A
authors.initials: A
authors.lastname: Paetau
authors.forename: E
authors.initials: E
authors.lastname: Saksela
authors.forename: T
authors.initials: T
authors.lastname: Timonen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001826:Body Fluids; D001932:Brain Neoplasms / Q000628:therapy*; D002478:Cells, Cultured; D002648:Child; D005260:Female; D005910:Glioma / Q000628:therapy*; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology*; D008222:Lymphokines / Q000502:physiology*; D008297:Male
chemical_list: D008222:Lymphokines