title: Myocyte reactions at the borders of injured and healing rat myocardium.
abstract: To better understand the apparent tendency of myocardium to heal by scarring rather than by restoration of normal structure, we have examined by light and electron microscopy the basic reactions of rat ventricular myocytes and their interactions with the extracellular matrix. Using three different types of necrotizing injuries, we found that necrotic myocytes separated from viable myocytes at intercalated discs leaving blunt-ended stumps at the edge of each lesion; the basal lamina of necrotic myocytes remained largely intact and spanned the gap between viable myocytes on opposite sides of each lesion. A small number of stumps were capped off by a new layer of basal lamina and showed no evidence of proliferative activity. The majority of the stumps developed cell processes that extended along the acellular myocyte basal lamina sheaths. These processes had one of two different fates. Some became apposed to similar processes, formed intercalated disc attachments, increased myofibrillar mass, and appeared to be associated with muscle reconstruction. Others developed elongate tapered ends, which terminated in myotendinous connections to scar tissue. The outcome of healing necrotic myocardium, like the healing of noncardiac necrotic tissue injuries, appears to be a function of cell growth and extracellular framework guidance; however, unlike healing of noncardiac tissues, healing of myocardium is uniquely complicated by continuing muscle contractions. We think the rhythmic pulling at the edges of necrotic lesions induces formation of myotendinous attachments, which anchor myocytes to scar tissue and probably prevent further growth.
authors.affiliation: Laboratory Service, Veterans Administration Medical Center, Seattle, Washington.
authors.forename: R
authors.initials: R
authors.lastname: Vracko
authors.forename: D
authors.initials: D
authors.lastname: Thorning
authors.forename: R G
authors.initials: RG
authors.lastname: Frederickson
authors.forename: D
authors.initials: D
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000473:pathology / Q000502:physiology; D003327:Coronary Disease / Q000473:pathology*; D005260:Female; D005615:Freezing; D006321:Heart / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000473:pathology* / Q000648:ultrastructure; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D012038:Regeneration
chemical_list: D007545:Isoproterenol