[Effect of isopropanol on the enzymatic activity and stability of thrombin].
Isopropanol is shown to affect considerably the thrombin activity. Its low concentrations (5-15%) activate the hydrolysis reaction of benzoyl-1-arginine ethyl ester (BAEE) by thrombin, whereas incomplete uncompetitive inhibition of the enzyme is observed in the presence of 20% isopropanol. Alcohol in the concentration of 25% results in complete reversible inhibition of the clothing and esterase activity of the enzyme. Isopropanol in the concentration of 25% is able to suppress the thrombin autolysis and therefore it may be used as a reagent which stabilizes thrombin during its storage in the aquatic-salt solutions.
Shvachko
L P
LP
Kibirev
V K
VK
D000433:1-Propanol / Q000494:pharmacology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000433:1-Propanol; D013917:Thrombin
Vliianie izopropanola na fermentativnuiu aktivnost' i stabil'nost' trombina.