1H and 31P NMR studies of the binding of low-affinity anions to Cu,Zn superoxide dismutase.
Anions that do not coordinate to the catalytically active copper ion of Cu,Zn superoxide dismutase, but still affect the activity of the enzyme by weaker interactions with the protein moiety surrounding the active site (low affinity anions), uniformly perturbed the 1H NMR line of the NH group of the copper ligand His 46. This effect was detected on the enzyme having Co(II) substituted for the native Zn(II), in which the resonances of residues bound to the copper are detected because of the antiferromagnetic coupling between Cu(II) and Co(II). The interaction with the enzyme of phosphate, a good representative of low-affinity anions, was also studied by 31P NMR of the native enzyme and of enzyme samples covalently modified at all lysines or at the Arg 141, which is 5 A away from the copper. The results obtained indicate that Arg 141 is a likely candidate for binding of low-affinity anions in the vicinity of the copper and that the 1H NMR line of His 46 NH is diagnostic for such an interaction.
Desideri
A
A
Department of Biology Tor Vergata University of Rome, Italy.
Paci
M
M
Rotilio
G
G
D000818:Animals; D000838:Anions; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology; D006859:Hydrogen; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010758:Phosphorus; D011485:Protein Binding; D013482:Superoxide Dismutase / Q000097:blood*
D000838:Anions; D010758:Phosphorus; D006859:Hydrogen; D013482:Superoxide Dismutase