Transmission electron microscopy of small numbers of sorted cells.
A new method that allows the transmission electron microscopic examination of as few as 1 x 10(4) cells obtained by flow cytometric sorting is described. The approach involves "sandwiching" fixed cells in an agarose case by a microcentrifugation system consisting of small-diameter cell-centrifugation tubes and subsequent processing of the cells by conventional techniques. The advantages offered by this method are discussed.
Sebring
R J
RJ
Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Johnson
N F
NF
Spall
W D
WD
D000818:Animals; D002469:Cell Separation; D005434:Flow Cytometry / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D051381:Rats; D014132:Trachea / Q000166:cytology* / Q000648:ultrastructure