Interferences in radioimmunoassay of aflatoxins in food and fodder samples of plant origin.
Cross-reactions and resulting nonspecific binding of substances with structures resembling aflatoxins (derivatives of coumarin, and cinnamonic and benzoic acids, etc.) were investigated. The concentrations of these substances causing erroneously high or false positive values in radioimmunoassay were determined. One microgram aflatoxin B1/kg sample may be simulated by the occurrence of 5 g coumarin, 10 g caffeic acid, 16 g chlorogenic acid, or 15 g vanillin/kg fodder or food sample.
Rauch
P
P
Institute of Chemical Technology, Department of Biochemistry and Microbiology, Prague, Czechoslovakia.
Fukal
L
L
Brezina
P
P
Kás
J
J
D000348:Aflatoxins / Q000032:analysis* / Q000276:immunology; D000821:Animal Feed / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007202:Indicators and Reagents; D011863:Radioimmunoassay; D012997:Solvents
D000348:Aflatoxins; D007202:Indicators and Reagents; D012997:Solvents