Purification of an antihemorrhagic factor from the serum of the non-venomous snake Dinodon semicarinatus.
An antihemorrhagic factor was purified from the serum of Dinodon semicarinatus, a non-venomous snake (Akamata), by a series of high performance liquid chromatographies with a TSK gel DEAE-5PW column. The purified antihemorrhagic factor showed a single band on polyacrylamide gel disc electrophoresis and inhibited the hemorrhagic activity of HR1 and HR2, the hemorrhagic factors of Trimeresurus flavoviridis Okinawa. The antihemorrhagic factor was stable from 0 degrees to 60 degrees and at pH values 2.0-11.0. The molecular weight of the factor was estimated to be 59,000 and 52,000 by a gel filtration and SDS-disc electrophoresis, respectively, suggesting that it consists of a single subunit, as we also found for the antihemorrhagic factors of the mongoose Herpestes edwardsii.
Tomihara
Y
Y
Division of Venomous Snakes, Okinawa Prefectural Institute of Public Health, Urasoe, Japan.
Yonaha
K
K
Nozaki
M
M
Yamakawa
M
M
Kawamura
Y
Y
Kamura
T
T
Toyama
S
S
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D012911:Snakes / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; C003941:antihemorrhagic factor