Hybridoma cultivation in defined serum-free media: growth-supporting substances. V. Trace elements.
The effects of selenium and manganese on growth of hybridomas in serum-free medium were studied. The optimum growth-supporting concentration of Na2SeO3 was about 50 nM. Omission of selenite resulted in decreased growth (60-70% of the number of cells growing with selenite when cultivated for 3 days). The optimum growth-supporting concentration of MnCl2 was 0.5 nM. The number of hybridoma cells growing 3 days without MnCl2 represented about 80% of the cell number obtained by cultivation with MnCl2. Compounds of other trace elements did not display growth-stimulating activity, when tested in short-term culture, perhaps with the exception of lithium at relatively very high concentrations (about 0.2-0.6 mM).
Kovár
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002712:Chlorides; D003470:Culture Media; D006825:Hybridomas / Q000166:cytology* / Q000187:drug effects; D008094:Lithium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology*; D017895:Manganese Compounds; D012643:Selenium / Q000494:pharmacology*; D018036:Selenium Compounds; D064588:Selenium Oxides; D014131:Trace Elements / Q000494:pharmacology*
D002712:Chlorides; D003470:Culture Media; D017895:Manganese Compounds; D018036:Selenium Compounds; D064588:Selenium Oxides; D014131:Trace Elements; D008345:Manganese; D008094:Lithium; D012643:Selenium; C025340:manganese chloride