Fluorescence high performance liquid chromatographic analysis of free fatty acid levels changed during aggregation of rat platelets.
Stimuli-induced changes in free fatty acid levels of activated platelets were assayed by reversed-phase high performance liquid chromatography. The fatty acids were prelabeled as their fluorescent 9-aminophenanthrene derivatives for the detection. The levels of saturated fatty acids such as palmitic acid in an extracellular medium of rat platelet-rich plasma are significantly raised by stimulation with 10 microM ADP or 8 micrograms/ml collagen. Similar increases in saturated fatty acids liberated from washed rat platelets are also observed by using thrombin stimulation. Quantitative changes in the intra- and extracellular levels of fatty acids induced by washed platelet aggregation were assayed after addition of thrombin for 15 min. Increase in palmitic and stearic acid was observed approximately 3-fold their levels of that the unstimulated control.
Ikeda
M
M
Division of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
Matsumoto
U
U
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000032:analysis* / Q000378:metabolism; D066298:In Vitro Techniques; D009227:Myristic Acids / Q000032:analysis / Q000378:metabolism; D010169:Palmitic Acids / Q000032:analysis / Q000378:metabolism; D010974:Platelet Aggregation; D051381:Rats; D011919:Rats, Inbred Strains; D013229:Stearic Acids / Q000032:analysis / Q000378:metabolism; D013917:Thrombin
D005230:Fatty Acids, Nonesterified; D009227:Myristic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D000244:Adenosine Diphosphate; D003094:Collagen; D013917:Thrombin