The inhibitory effects of teniposide and homoharringtonine on the growth of pancreatic carcinoma cells in vitro.
The inhibitory effect of VM-26 and Homoharringtonine (HHT) on the in vitro growth of a human pancreatic cell line (MIA PaCa-2) was tested. The ID50 of VM-26 and HHT was 6 x 10(-7) M and 2 x 10(-5) M respectively. Increasing time exposure from 1 hr to continuous exposure for 5 days resulted in a 30-fold decrease in ID50 for VM-26 and a 2,000-fold decrease for HHT. 14C-labelled thymidine and leucine studies revealed that VM-26 inhibited DNA synthesis, while HHT inhibited both DNA and protein synthesis.
Fountzilas
G
G
First Department of Medicine, AHEPA University Hospital, Thessaloniki, Greece.
Lim
L O
LO
Yunis
A A
AA
D000470:Alkaloids / Q000494:pharmacology*; D002277:Carcinoma / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D003864:Depression, Chemical; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010190:Pancreatic Neoplasms / Q000473:pathology*; D011034:Podophyllotoxin / Q000031:analogs & derivatives*; D013713:Teniposide / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000470:Alkaloids; D006248:Harringtonines; D009363:Neoplasm Proteins; D000077863:Homoharringtonine; D013713:Teniposide; D011034:Podophyllotoxin