title: Unilocular fat cells in three-dimensional collagen gel matrix culture.
abstract: Three-dimensional culture with collagen gel, developed recently for the in vitro study of some mammalian cells in a more physiological condition than a monolayer culture, was applied for a biological study of unilocular fat cells. Successfully embedded in the gel, the unilocular fat cells were shown to be able to keep their cellular function and actively proliferate. These findings confirm that unilocular fat cells do undergo proliferation under in vitro conditions as demonstrated in monolayer culture.
authors.affiliation: Department of Pathology, Saga Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sugihara
authors.forename: N
authors.initials: N
authors.lastname: Yonemitsu
authors.forename: S
authors.initials: S
authors.lastname: Toda
authors.forename: S
authors.initials: S
authors.lastname: Miyabara
authors.forename: S
authors.initials: S
authors.lastname: Funatsumaru
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000293:Adolescent; D000328:Adult; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D005109:Extracellular Matrix; D005260:Female; D006801:Humans; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003470:Culture Media; D003094:Collagen