Isolation of cDNA clones of human argininosuccinate lyase and corrected amino acid sequence.
In the present study, we isolated clones of human argininosuccinate lyase (ASL) cDNA from a liver cDNA library using a clone of rat ASL cDNA and analyzed human ASL cDNA nucleotide sequence. The results reveal that the sequence of human ASL cDNA published by O'Brien et al. in 1986 [Proc. Natl. Acad. Sci USA 83, 7211-7215] had one-base deletions at three independent positions in the coding regions near the COOH-terminus, which caused frame-shift variations in the amino acid sequence. Amino acid sequencing of peptides prepared from purified human liver ASL showed our predicted amino acid sequence to be correct.
Matuo
S
S
Department of Biochemistry, Faculty of Medicine, Kagoshima Univeristy, Japan.
Tatsuno
M
M
Kobayashi
K
K
Saheki
T
T
Miyata
T
T
Iwanaga
S
S
Amaya
Y
Y
Mori
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001123:Argininosuccinate Lyase / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005796:Genes; D006801:Humans; D008099:Liver / Q000201:enzymology; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats
D004247:DNA; D008190:Lyases; D001123:Argininosuccinate Lyase