A colorimetric procedure to monitor the extent of crosslinking of proteins by bisimidoesters.
Reaction of proteins with bisimidoesters is one of the popular crosslinking techniques. The extent of the reaction cannot be followed directly using the commonly used free amino group-determining reagents ninhydrin and 2,4,6-trinitrobenzene sulfonic acid since they react with bisimidoesters. However, we found that the extent of amidination of protein could be followed colorimetrically by using 1-fluoro-2,4-dinitrobenzene.
Kamra
A
A
Chemistry Department, Indian Institute of Technology, New Delhi.
Gupta
M N
MN
D000818:Animals; D002417:Cattle; D003124:Colorimetry / Q000379:methods*; D003432:Cross-Linking Reagents; D004139:Dinitrofluorobenzene; D009578:Nitrobenzenes; D011506:Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate
D003432:Cross-Linking Reagents; D009578:Nitrobenzenes; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D004139:Dinitrofluorobenzene