An in vivo chicken model for peripheral intravascular human fibrin clot detection.
A chicken model was prepared that provides a simple and economical method of evaluating the use of fibrin-specific monoclonal antibody 64C5 in the detection of peripheral vascular thrombi. Human fibrin was clotted in segments of a chicken's femoral artery and vein prior to intravenous injection of radioiodinated antibody 64C5. After a 3-hr perfusion time, the thrombosed and contralateral control segments of the vessels were excised and counted for radioactivity. The radiolabeled 64C5 uptake ratio of the thrombosed segment to the control segment was 5.4 +/- 1.2 (p less than 0.007) in the femoral artery, and 3.8 +/- 1.1 (p less than 0.02) in the femoral vein. This in vivo chicken model may also find application in studies of targeting agents for human fibrin.
Hui
K Y
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Cellular and Molecular Research Laboratory, Massachusetts General Hospital, Boston.
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens / Q000097:blood*; D004195:Disease Models, Animal; D004903:Erythrocyte Aggregation / Q000097:blood*; D005337:Fibrin / Q000502:physiology*; D006801:Humans; D007457:Iodine Radioisotopes; D013927:Thrombosis / Q000097:blood / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D005337:Fibrin