Complex-type glycoproteins synthesized in the subcommissural organ of mammals. Light- and electron-microscopic investigations by use of lectins.
The secretory activity in the subcommissural organ (SCO) of the sheep and cow was examined by means of lectin histochemistry and cytochemistry. Among the various lectins tested. Concanavalin A (Con A) revealed glycoproteins rich in mannosyl residues in the rough endoplasmic reticulum of ependymal and hypendymal cells. One of these Con A-positive glycoproteins may represent the precursor of the specific secretory component elaborated in the SCO, giving rise to Reissner's fiber. Lens culinaris agglutinin (LCA) and Phaseolus vulgaris hemagglutinins (E-PHA and L-PHA), known to bind to oligosaccharides, as well as wheat-germ agglutinin (WGA) revealing neuraminic acid, labeled secretory granules located in the apical part of ependymal and hypendymal cells of ruminants, and also Reissner's fiber. Electron-microscopic visualization of WGA-positive material in the Golgi complex shows that complex-type glycoproteins are synthesized in the subcommissural organ of mammals. The electron-dense material is mainly secreted into the ventricular cavity and gives rise to Reissner's fiber. On the basis of lectin affinity for oligosaccharides, a structure of the complex-type oligosaccharide is proposed.
Meiniel
A
A
Laboratoire de Biologie animale et UA CNRS 677, Université Blaise Pascal, Clermont-Ferrand, France.
Molat
J L
JL
Meiniel
R
R
D000818:Animals; D002417:Cattle; D003208:Concanavalin A; D006023:Glycoproteins / Q000378:metabolism* / Q000502:physiology; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009490:Neurosecretory Systems / Q000378:metabolism*; D010835:Phytohemagglutinins; D012756:Sheep; D013351:Subcommissural Organ / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D010835:Phytohemagglutinins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A