Large-scale fractionation of S-form lipopolysaccharide from Salmonella abortus equi. Chemical and serological characterization of the fractions.
The S-form lipopolysaccharide of Salmonella abortus equi was separated by a newly elaborated extraction method with organic solvents into three fractions of different chain length of the O-polysaccharide they contained. The three fractions were designated long-chain (20-50 repeating units), short-chain (0-6) and R-fraction (no repeating units) according to their migration pattern in polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate. The nature of the fractions as long- and short-chain and as R-fraction was confirmed by chemical analysis. The concentration of O-specific sugars was highest in the long-chain fraction, where their molar ratio to glucosamine was ca. 25:1. In the short-chain fraction the ratio of O-sugars to glucosamine was 2.5:1, and in the R-fraction O-specific sugars were absent. The serological properties of the three fractions were in good agreement with their chemical composition.
Galanos
C
C
Max-Planck-Institut für Immunobiologie, Freiburg, F.R.G.
Jiao
B H
BH
Komuro
T
T
Freudenberg
M A
MA
Lüderitz
O
O
D002241:Carbohydrates / Q000032:analysis; D003124:Colorimetry; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology / Q000302:isolation & purification*; D012475:Salmonella / Q000032:analysis* / Q000276:immunology
D002241:Carbohydrates; D005227:Fatty Acids; D008070:Lipopolysaccharides