Identification of a new seventh complementation group of UV-sensitive mutants in Chinese hamster cells.
The UV-sensitive mutant V-B11, isolated from the V79 Chinese hamster cell line (Zdzienicka and Simons, 1987) was further characterized. V-B11 has a slightly increased cross-sensitivity to 3me4NQO, whereas no increased sensitivity towards 4NQO was observed. A slightly increased sensitivity towards EMS and MMS was also found. The mutant shows a defect in the ability to perform the incision step of nucleotide-excision repair after UV irradiation: 2 h after UV exposure, the accumulation of incision breaks in V-B11, in the presence of HU and araC, was about 30% of that found in wild-type V79 cells. V-B11 was crossed to a panel of 6 UV-sensitive Chinese hamster ovary (CHO) cells, which represents all the previously identified 6 complementation groups of UV-sensitive Chinese hamster mutants. Since in all crosses complementation has been observed, V-B11 appears to be the first mutant of a new, 7th, complementation group.
Zdzienicka
M Z
MZ
Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
van der Schans
G P
GP
Simons
J W
JW
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005816:Genetic Complementation Test; D006822:Hybrid Cells / Q000528:radiation effects; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded; D009153:Mutagens