title: Production of a specific antibody against pyruvate kinase type M2 using a synthetic peptide.
abstract: The pyruvate kinase isozymes M1 and M2 are structurally and immunologically closely related. To obtain an antibody which discriminates between these two forms, a synthetic tetradecapeptide with a sequence specific for pyruvate kinase type M2 from rats was constructed. Antisera from rabbits, immunized with this peptide, reacted specifically with the M2-type holoenzyme of both rat and human origin, and did not cross-react with the M1-type isozyme. This was established by immunoblot analysis, both under dissociating and non-dissociating conditions.
authors.affiliation: Department of Haematology, University Hospital Utrecht, The Netherlands.
authors.forename: P A
authors.initials: PA
authors.lastname: Oude Weernink
authors.forename: G
authors.initials: G
authors.lastname: Rijksen
authors.forename: G E
authors.initials: GE
authors.lastname: Staal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D007527:Isoenzymes / Q000276:immunology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology; D011770:Pyruvate Kinase / Q000276:immunology*; D051381:Rats
chemical_list: D000906:Antibodies; D007527:Isoenzymes; D009842:Oligopeptides; D011770:Pyruvate Kinase