Effect of short-duration progesterone treatment on decidual prolactin production by cultures of proliferative human endometrium.
The effect of short-term progesterone (P) treatment in vitro on decidual prolactin (dPRL) production by human endometrium was investigated. Cultures prepared from proliferative endometrium received medium containing P for 3 hours, 1 day, or 3 days. The culture medium was then changed daily for 7 to 14 days, and the amounts of dPRL in the spent medium were measured by radioimmunoassay. The results of these experiments indicated that even a short exposure to elevated concentrations of P is adequate to stimulate dPRL production and that the resulting pattern of dPRL production is determined by the duration of the P treatment.
Maslar
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Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton 97006.
Ansbacher
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D002478:Cells, Cultured; D003656:Decidua / Q000378:metabolism*; D005260:Female; D006801:Humans; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology*; D011388:Prolactin / Q000096:biosynthesis*; D013997:Time Factors
D011374:Progesterone; D011388:Prolactin