Molecular cloning, cDNA structure and deduced amino acid sequence for the hormone-induced regulatory subunit (RII beta) of cAMP-dependent protein kinase from rat ovarian granulosa cells.
The regulatory subunit of cAMP-dependent protein kinase designated RII beta (RII51) has previously been shown to be the product of a separate gene. This was accomplished by the molecular cloning of a partial cDNA clone estimated to lack 30-45 nucleotides of the 5' end of the coding region. We hereby report the isolation of a cDNA clone for RII beta from rat granulosa cells, extending 43 nucleotides further 5' compared with the previously published cDNA sequence, and from which the entire amino acid sequence (415 residues) of the rat RII beta protein can be deduced. A cAMP regulated mRNA of 3.2 kilobases (kb) for RII beta was detected by the isolated cDNA in rat Sertoli cells.
Sandberg
M
M
Institute of Pathology, University of Oslo, Rikshospitalet, Norway.
Levy
F O
FO
Oyen
O
O
Hansson
V
V
Jahnsen
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005260:Female; D006107:Granulosa Cells / Q000201:enzymology*; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000032:analysis / Q000235:genetics*; D051381:Rats
D004247:DNA; D011494:Protein Kinases