[Somatotropin increases the rate of incorporation of thymidine into DNA and amino acids into proteins of thymocyte subpopulations isolated in a ficoll-urografin gradient from hypothyroid rats].
The summarized suspension of thymocytes was separated into subpopulations in the ficoll-urografin gradient density and 6 fractions of thymocytes were obtained. Experiments in vivo showed that under hypothyreosis incorporation of 3H-thymidine into DNA thymocytes, which were isolated in the ficoll-urografin density of 1.065, 1.071, 1.073 and residue is found to be increased than that of intact rats by 72, 35, 47 and 24%, respectively, and incorporation of 14C-amino acids into thymocyte proteins--by 44, 29, 37 and 45%, respectively. Somatotropin normalizes incorporation of the mentioned precursors into the corresponding biopolymers.
Litvienko
E A
EA
Martynenko
F P
FP
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000378:metabolism*; D003974:Diatrizoate Meglumine; D005362:Ficoll; D013006:Growth Hormone / Q000494:pharmacology*; D007037:Hypothyroidism / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism* / Q000473:pathology
D000596:Amino Acids; D011506:Proteins; D005362:Ficoll; D003974:Diatrizoate Meglumine; D013006:Growth Hormone; D004247:DNA; D013936:Thymidine
Somatotropin povyshaet skorost' vkliucheniia timidiba v DNK i aminokislot v belki subpopuliatsii timotsitov, vydelennykh v gradiente fikoll-urografin, u gipotireoidnykh krys.