Analysis of the cytogenetic effect in human lymphocytes induced by metabolically activated 1- and 2-methylnaphthalene.
Chromosome analyses were carried out in human lymphocytes treated in vitro with 1- and 2-methylnaphthalene (1-MN, 2-MN) in the presence and absence of the mammalian metabolic activation system, S9 mix. Without S9 mix there was no indication of induction of any significant cytogenetic effect by either compound. With S9 mix a weak clastogenic effect was apparent at 4 mM 2-MN only and sister-chromatid exchange frequencies were significantly increased at each dose of 1- and 2-MN, yet always less than twice the control level. The present observations do not indicate that 1- and 2-MN must be classified as potential genotoxic substances.
Kulka
U
U
Institut für Strahlenbiologie, GSF, Neuherberg, F.R.G.
Schmid
E
E
Huber
R
R
Bauchinger
M
M
D001711:Biotransformation; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D003520:Cyclophosphamide / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects*; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009281:Naphthalenes / Q000378:metabolism / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects
D009281:Naphthalenes; D003520:Cyclophosphamide; C025968:1-methylnaphthalene; C027384:2-methylnaphthalene