Effects of training on erythrocyte 2,3-diphosphoglycerate in normal men.
The erythrocyte 2,3-diphosphoglycerate concentration (2,3-DPG) and the activity of red cell hexokinase, pyruvate kinase, glucose-6 phosphate dehydrogenase and glutathione reductase were studied in 27 normal volunteers before and after 2 and 4 months of physical endurance training. The 4 months of training increased maximal oxygen uptake and physical working capacity (PWC130) by 16% (p less than 0.001) and 29% (p less than 0.001) respectively. Resting heart rate was decreased (p less than 0.001) by 11 beats.min-1. With 2 months of training the erythrocyte 2,3-DPG concentration increased by 9% (p less than 0.001); with 4 months training the increase was only 4% (p less than 0.05). The training-induced increase in red cell 2,3-DPG was not accompanied by enhanced activity of erythrocyte hexokinase, pyruvate kinase, glucose-6 phosphate dehydrogenase or glutathione reductase. It is concluded that the rise in red cell 2,3-DPG induced by physical endurance training is not due to activation of red cell glycolytic enzymes or the enzymes involved in the pentose-phosphate cycle.
Hespel
P
P
Department of Pathophysiology, University of Leuven (K. U.-Leuven), Belgium.
Lijnen
P
P
Fagard
R
R
Van Hoof
R
R
Goossens
W
W
Amery
A
A
D019794:2,3-Diphosphoglycerate; D000328:Adult; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010806:Physical Education and Training; D010807:Physical Endurance; D012016:Reference Values
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate