The analysis of urinary meta- and para-tyramine by gas chromatography with electron-capture detection.
A simple, reliable method for the analysis of urinary meta- and para-tyramine has been developed. Sample purification was achieved by a weak anion-exchange column, followed by extraction into ethyl acetate at pH 10.2. The heptafluorobutyryl derivative was measured by packed column gas chromatography with electron-capture detection, using para-hydroxyphenylpropylamine as internal standard. The results agreed well with those obtained by gas chromatography-mass spectrometry. The daily output of unconjugated and conjugated meta- and para-tyramine by 23 adults was measured. In consecutive 24 h urine samples from a single subject there was little day-to-day variation in the level of excretion of unconjugated meta- and para-tyramine, whereas the conjugated amines exhibited marked fluctuations.
Walker
P L
PL
Department of Clinical Biochemistry, Pinderfields General Hospital, Wakefield, West Yorks, UK.
Sandler
M
M
D000328:Adult; D002648:Child; D002849:Chromatography, Gas; D004563:Electrochemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D007231:Infant, Newborn; D014439:Tyramine / Q000652:urine*
D007202:Indicators and Reagents; C028169:3-tyramine; D014439:Tyramine