title: Effect of GPIIb-IIIa complex ligands on calcium ion movement and cytoskeleton organization in activated platelets.
abstract: We studied the influence of the occupancy of the fibrinogen receptor (GP IIb-IIIa complex) on two early aspects of agonist induced platelet activation: the increase of the intracellular Ca2+ concentration and the cytoskeleton reorganization. A monoclonal antibody, a peptide containing the RGD sequence and fibrinogen purified from human plasma were used as GP IIb-IIIa ligands. The obtained results demonstrated that fibrinogen receptor occupancy inhibits Ca2+ movement and cytoskeleton reorganization caused by low thrombin concentration and ADP.
authors.affiliation: Department of Biochemistry, University of Pavia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Sinigaglia
authors.forename: A
authors.initials: A
authors.lastname: Bisio
authors.forename: M
authors.initials: M
authors.lastname: Torti
authors.forename: C L
authors.initials: CL
authors.lastname: Balduini
authors.forename: G
authors.initials: G
authors.lastname: Bertolino
authors.forename: C
authors.initials: C
authors.lastname: Balduini
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D002118:Calcium / Q000097:blood*; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000502:physiology*; D013917:Thrombin / Q000502:physiology
chemical_list: D010980:Platelet Membrane Glycoproteins; D000244:Adenosine Diphosphate; D005340:Fibrinogen; D013917:Thrombin; D002118:Calcium