Sex identification in fresh blood and dried bloodstains by a nonisotopic deoxyribonucleic acid (DNA) analyzing technique.
Deoxyribonucleic acid (DNA) specimens were prepared from blood or bloodstain extracts, and the content of a Y-chromosome specific DNA fragment was investigated by the Southern hybridization method using a nonisotopic staining technique. Thus obtained patterns of male DNA showed a clear band, whereas broad stains with some faint bands appeared on the patterns of DNA from both sexes. This method is expected to be a new powerful mean of forensic medical examination.
Kobayashi
R
R
Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Nakauchi
H
H
Nakahori
Y
Y
Nakagome
Y
Y
Matsuzawa
S
S
D001345:Autoradiography; D001801:Blood Stains; D004247:DNA / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012732:Sex Determination Analysis
D004247:DNA