title: Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin).
abstract: Amino acid sequences of 285 and 286 residues, respectively, were deduced for mouse and human BM-40 from cDNA clones isolated from expression libraries. The sequences showed 92% identity and were also essentially identical to those of bone osteonectin and of the parietal endoderm protein SPARC. About 60% of the mouse BM-40 sequence was confirmed by Edman degradation. Two of the seven disulfide bonds were localized which apparently separate two distinct domains of mouse BM-40.
authors.affiliation: Dermatologische Klinik der Universität München, FRG.
authors.forename: B
authors.initials: B
authors.lastname: Lankat-Buttgereit
authors.forename: K
authors.initials: K
authors.lastname: Mann
authors.forename: R
authors.initials: R
authors.lastname: Deutzmann
authors.forename: R
authors.initials: R
authors.lastname: Timpl
authors.forename: T
authors.initials: T
authors.lastname: Krieg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001485:Basement Membrane / Q000032:analysis*; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004220:Disulfides; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D015676:Osteonectin
chemical_list: D002352:Carrier Proteins; D004220:Disulfides; D015676:Osteonectin; D004247:DNA