Human gamma enolase: isolation of a cDNA clone and expression in normal and tumor tissues of human origin.
We have isolated and sequenced a cDNA clone encoding the human gamma enolase. Comparison of our cDNA sequence and the rat gamma enolase sequence revealed 97% homology at the level of amino acid sequence. The two coding regions were 91% homologous on the nucleotide level, whereas the 3' noncoding regions were much less homologous (32%). Further comparison of our cDNA sequence with the human alpha enolase revealed an 82% homology at the amino acid level and a 75% homology at the nucleotide level for the two coding regions, whereas the 3' nontranslated regions were only 30% homologous. Using a portion of the 3' nontranslated region of our cDNA, shown to be specific for human gamma enolase, a single 2.5 kb mRNA was detected in human brain tissue. This same gamma enolase message was also found in a number of human normal nonneuronal tissues, and in several human tumor-derived cell lines. Expression of the mRNA for the gamma enolase subunit should thus be used with caution when identifying the cells of neuronal or neuroendocrine origin.
Van Obberghen
E
E
Laboratory of Molecular Genetics, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
Kamholz
J
J
Bishop
J G
JG
Zomzely-Neurath
C
C
Lazzarini
R A
RA
Lazzarini
R A
RA
D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D002460:Cell Line; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D012333:RNA, Messenger; D004247:DNA; D010751:Phosphopyruvate Hydratase