Inorganic phosphate and energy charge compartmentation in Ehrlich ascites tumour cells in the presence of glucose and/or glutamine.
Inorganic phosphate concentrations and the values of energy charge were determined in the cytosolic and mitochondrial compartments of Ehrlich ascites tumour cells incubated with 5 mM glucose and/or 0.5 mM glutamine. The initial value of inorganic phosphate concentration in the cytosolic compartment decreased in the three incubation conditions assayed; this decrease was greater when glucose was present in the incubation medium. Mitochondrial inorganic phosphate concentration diminished in incubations with only glucose and raised in incubations with only glutamine.
Medina
M A
MA
Departamento de Bioquímica, Biología Molecular y Química Orgánica, Facultad de Ciencias, Universidad de Málaga, Spain.
Quesada
A R
AR
Márquez
F J
FJ
Sánchez-Jiménez
F
F
Núñez de Castro
I
I
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D005973:Glutamine / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*
D010710:Phosphates; D005973:Glutamine; D005947:Glucose