Production of subtype-specific antipeptide antibodies to human interferon-alpha 1 and -alpha 4.
Antibodies that are specific to the human interferon (IFN)-alpha 1 and -alpha 4 subtypes have been produced by immunizing rabbits with two short synthetic peptides, corresponding to residues 99-111 of IFN-alpha 1 and residues 37-50 of IFN-alpha 4, respectively. The IFN-alpha 1 peptide has at least three closely clustered residues that are different from those in the other IFN-alpha subtypes, while the IFN-alpha 4 peptide has only two unique amino acid residues, separated by five common residues. The antibodies raised against the IFN-alpha 1 peptide react with recombinant human IFN-alpha 1 but do not cross-react with recombinant human IFN-alpha 4 or IFN-alpha 2. The antibodies raised against the IFN-alpha 4 peptide react with IFN-alpha 4, cross-react with IFN-alpha 1 but not with IFN-alpha 2; the affinity of the antibodies to IFN-alpha 1, however, is at least 10 times lower than their affinity to IFN-alpha 4.
Sattayasai
N
N
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Hibbs
A R
AR
McMullen
G L
GL
Linnane
A W
AW
Marzuki
S
S
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D003429:Cross Reactions; D007370:Interferon Type I / Q000145:classification / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology
D000906:Antibodies; D007370:Interferon Type I; D010455:Peptides; D011994:Recombinant Proteins