A bioluminescent assay for the determination of phosphoenolpyruvate carboxykinase activity in nanogram-sized tissue samples.
A highly specific and sensitive assay for the determination of phosphoenolpyruvate carboxykinase (PEPCK) in nanogram-sized tissue samples is described. This test system is based on the stoichiometric transformation of phosphoenolpyruvate into ATP. In a subsequent step ATP is quantified by bioluminescent techniques. The applicability of this assay system is shown by measurements in liver samples with normal and high PEPCK activity levels.
Wimmer
M
M
Department of Anatomy, University of Basle, Switzerland.
D000255:Adenosine Triphosphate / Q000032:analysis / Q000096:biosynthesis; D000818:Animals; D049454:Firefly Luciferin; D008099:Liver / Q000201:enzymology; D008156:Luciferases; D008297:Male; D008832:Microchemistry; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases; D010729:Phosphoenolpyruvate Carboxykinase (GTP)