Lyso-phosphatidylcholine is implicated in thioacetamide-induced liver necrosis.
Thioacetamide is a weak hepatocarcinogen. To determine whether alterations in lysophosphatidylcholine are implicated in thioacetamide-induced hepatic necrosis, rats were injected i.p. with this agent (50 mg/Kg body weight per day) or diluent for 1, 3, 8 and 30 days. Serum catalytic activities of aminotransferases were determined. Incorporation of (32P)-orthophosphate into hepatic lysophosphatidylcholine was also evaluated in animals killed 75 minutes or 13 hours after isotope administration. Results demonstrate that: A significant increase in hepatic lysolecithin concentration occurs when a maximum level of serum aminotransferases is present. An increase of (32P)-orthophosphate radioactive incorporation in lysolecithin was observed at the two assayed labelling periods, which suggest an activation of phospholipase A. The radioactivity present in lysolecithin after 13 h isotope injection showed a close correlation with serum level of aminotransferases. From these results it can be deduced that lysolecithin is implicated in TAA-induced necrosis and may be generated by increase in either phospholipase A activity and/or synthesis.
Osada
J
J
Instituto de Bioquìmica, Centro Mixto (C.S.I.C.-U.C.M.) Facultad de Farmacia Universidad Complutense, Madrid, España.
Aylagas
H
H
Mirò-Obradors
M J
MJ
Palacios-Alaiz
E
E
D000081:Acetamides / Q000633:toxicity*; D000818:Animals; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D008297:Male; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D013853:Thioacetamide / Q000633:toxicity*
D000081:Acetamides; D008244:Lysophosphatidylcholines; D013853:Thioacetamide