Detection of styrene oxide-DNA adducts by 32P-postlabeling.
In vitro reaction of DNA with styrene-7,8-oxide (styrene oxide) produced five adducts, as determined by 32P-postlabeling. When styrene oxide was reacted in vitro with deoxyribonucleotides, five adducts were observed from 2'-deoxyguanosine-3'-monophosphate, two from 2'-deoxyadenosine-3'-monophosphate, none from 2'-deoxythymidine-3-monophosphate or 2'-deoxycytidine-3'-monophosphate. Chromatographic comparison of the adducts formed in DNA with those formed with the deoxyribonucleotides suggests that deoxyguanosine is the primary site of DNA modification. Treatment of 9L cells with 1 mM styrene oxide resulted in the formation of several DNA adducts as detected by the postlabeling procedure. Our results indicate that 32P-postlabeling can be used to investigate DNA adducts formed by styrene oxide.
Liu
S F
SF
Department of Biomedical and Environmental Health Sciences, School of Public Health, University of California, Berkeley 94720.
Rappaport
S M
SM
Rasmussen
J
J
Bodell
W J
WJ
D004247:DNA / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D004988:Ethers, Cyclic / Q000378:metabolism*; D006147:Guanine / Q000378:metabolism; D010761:Phosphorus Radioisotopes
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D010761:Phosphorus Radioisotopes; D006147:Guanine; D004247:DNA; C013690:styrene oxide