[Recording and interpretation of the results obtained by using the ELISA Sevatest commercial immunoenzyme kit for detecting Toxoplasma gondii antibodies].
The results of the repeated tests of 295 serum samples from patients, previously examined by means of enzyme immunoassay screening and traditional serological tests (complement fixation, indirect immunofluorescence, and indirect hemagglutination) and 115 serum samples from healthy donors, studied by enzyme immunoassay techniques with the use of the commercial kits Sevatest ELISA, have been analyzed. The methodological approach permitting the determination of the final titer of the serum under study, taken in a dilution of 1:800, by its optical density has been used. The mean geometric titer for the control group of donors, determined in the enzyme immunoassay, has been 1:800. This fact suggests that titers exceeding 1:1,600 should be considered diagnostically significant.
Moroz
B V
BV
Ponomareva
S I
SI
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012698:Serologic Tests / Q000295:instrumentation / Q000379:methods; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis
D000913:Antibodies, Protozoan; D011933:Reagent Kits, Diagnostic
Uchet i interpretatsiia rezul'tatov, poluchaemykh pri ispol'zovanii kommercheskogo immunofermentnogo nabora Sevatest ELISA dlia obnaruzheniia antitel k Toxoplasma gondii.