Late effects of fractionated pi-mesons compared to X rays on mouse lung.
Early and late delayed effects of up to 20 fractions of pions and X rays were investigated in the mouse lung. The whole thorax of female CD-1 mice was irradiated under Ethrane/O2 anesthesia. Respiration rate was measured by whole body plethysmography at biweekly to monthly intervals. With signs of irreversible respiratory distress, animals were sacrificed and their lungs evaluated histologically. In addition to the effect of fractionation, the influence of dose-rate and anesthesia was studied as well. The degree of injury for the most predominant lesions (macrophage accumulation, fibrosis, vascular congestion) was scored, and the correlation with the relative change in respiratory rate and survival was analyzed. This analysis showed the primary lesion to be radiation pneumonitis at a median survival time of approximately 100 days. Focal fibrosis was observed to occur soon thereafter, and no evidence was obtained for an independent second wave of fibrotic injury. Fibrosis seemed primarily the result of pathological organization in areas with heavy concentration of macrophages. It was observed that the mice were unusually sensitive, with a single dose X ray ED50/180 of 8.8 Gy. A similar value was found for unanesthetized mice. This might have been the result of performing these studies at an altitude of 2100 m. The fractionation effect also seemed more pronounced, with alpha/beta values of 0.6 Gy for X rays and 4 Gy for pions, which is significantly lower compared to reported values. At the pion dose-rate of 0.25 Gy.min-1, RBE values for single doses were 0.9 when compared to high dose-rate X rays, and 1.36 at equivalent dose rates. This clearly shows that significant repair occurs during the relatively low dose-rate pion irradiations. With smaller doses per fraction, the dose-rate effect became less dominant, and for 20 fractions of pions the RBE was 1.4 compared to fractionated high dose-rate X rays. These RBE's are similar to values reported for acute effects in skin.
van der Kogel
A J
AJ
Los Alamos National Laboratory, Life Sciences Division, NM.
Martin
J C
JC
Smith
A R
AR
Raju
M R
MR
D000208:Acute Disease; D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004601:Elementary Particles; D004735:Energy Transfer; D005260:Female; D008168:Lung / Q000503:physiopathology / Q000528:radiation effects*; D008651:Mesons; D051379:Mice; D008815:Mice, Inbred Strains; D011014:Pneumonia / Q000209:etiology / Q000401:mortality / Q000503:physiopathology; D011833:Radiation Injuries, Experimental / Q000401:mortality / Q000503:physiopathology; D012119:Respiration / Q000528:radiation effects; D013997:Time Factors