title: Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene.
abstract: The gene for staphylococcal enterotoxin type E (entE) was cloned from Staphylococcus aureus into plasmid vector pBR322 and introduced into Escherichia coli. A staphylococcal enterotoxin type E-producing E. coli strain was isolated. The complete nucleotide sequence of the cloned structural entE gene and the N-terminal amino acid sequence of mature staphylococcal enterotoxin type E were determined. The entE gene contained 771 base pairs that encoded a protein with a molecular weight of 29,358 which was apparently processed to a mature extracellular form with a molecular weight of 26,425. DNA sequence comparisons indicated that staphylococcal enterotoxins type E and A are closely related. There was 84% nucleotide sequence homology between entE and the gene for staphylococcal enterotoxin type A; these genes encoded protein products that had 214 (83%) homologous amino acid residues (mature forms had 188 [82%] homologous amino acid residues).
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison 53706.
authors.forename: J L
authors.initials: JL
authors.lastname: Couch
authors.forename: M T
authors.initials: MT
authors.lastname: Soltis
authors.forename: M J
authors.initials: MJ
authors.lastname: Betley
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins; C049463:enterotoxin E, Staphylococcal; C026066:enterotoxin A, Staphylococcal