Androgen regulation of murine beta-glucuronidase expression: identification and characterization of a nonresponse variant.
One of the major features of beta-glucuronidase (GUS) expression in inbred strains of the house mouse, Mus musculus, is the responsiveness of this enzyme to androgen stimulation in tubule cells of the kidney. Both GUS-specific and nonspecific mutations have been described which define genes that serve to control this response. During examination of the expression of GUS in the interbreeding subspecies, Mus hortulanus, a new GUS haplotype was uncovered that is characterized, in part, by a lack of GUS response to androgen stimulation in an apparently responsive kidney. Blot hybridization analyses of kidney RNA with a radiolabeled murine GUS cDNA shows this lack of response to be reflected in GUS mRNA levels. The difference in heat stability of GUS activity between M. hortulanus and a responsive inbred strain, ICR/Ha, was utilized to assess the contribution of each parent to kidney levels of GUS in androgen-treated and -untreated F1 progeny of these strains. The results, together with preliminary genetic studies, suggest that the element controlling this responsiveness (or the lack thereof) is cis-active and tightly linked to the GUS structural gene on chromosome 5. It is not known whether this element is identical to another GUS-specific, cis-active element, Gus-r, which also controls the androgen response of GUS in mouse kidney.
Lund
S D
SD
Division of Basic Science Research, Children's Hospital Research Foundation, Cincinnati, Ohio 45229.
Miller
D
D
Chapman
V
V
Ganschow
R E
RE
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D004789:Enzyme Activation; D005966:Glucuronidase / Q000037:antagonists & inhibitors / Q000235:genetics*; D007684:Kidney Tubules / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D013045:Species Specificity
D000728:Androgens; D005966:Glucuronidase