[Allergen analysis of mugwort pollen (Artemisia vulgaris) using crossed radioimmunoelectrophoresis and radioallergosorbent test inhibition].
It could be identified 43 antigens of mugwort pollen (Artemisia vulgaris), 31 migrating anodically and 12 cathodically, by means of crossed immunoelectrophoresis (CIE). Eleven antigens bound human IgE-antibodies in crossed radioimmunoelectrophoresis (CRIE). Two major allergens could be found, allergens 23 and 40.- The allergenic activity of mugwort pollen extract was examined by means of RAST-inhibition. Specificity of this reaction could be determined by a control trial with grass pollen extract.
Diener
C
C
Institut für Klinische Immunologie, Friedrich-Schiller-Universität Jena.
Frenzel
K
K
Wiebicke
K
K
Schlenvoigt
G
G
Fuster
R
R
Jäger
L
L
D000485:Allergens / Q000276:immunology*; D001797:Blood Protein Electrophoresis; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test; D011863:Radioimmunoassay; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*
D000485:Allergens; D007073:Immunoglobulin E
Allergenanalyse von Beifusspollen (Artemisia vulgaris) mittels gekreuzter Radioimmunelektrophorese (CRIE) und RAST-Hemmung.