title: [Various properties of cathepsin G and elastase from swine peripheral blood neutrophils].
abstract: Using gel filtration through Sephadex G-100 and bioaffinity chromatography on contrical-Sepharose, cathepsin G and elastase were isolated from pig peripheral blood neutrophil granules and purified to homogeneity. Both enzymes hydrolyzed the total histone from calf thymus as well as synthetic substrates--tert-butoxy-L-alanine p-nitrophenyl ester (elastase) and benzoyltyrosine ethyl ester (cathepsin G). The use of natural and synthetic protease inhibitors showed that both enzymes were related to the group of serine proteases. The molecular mass of the cathepsin G subunit as determined by SDS polyacrylamide gel electrophoresis is 28-29 kD, that of elastase--30-31 kD. The pH optima for the hydrolysis of proteinaceous and synthetic substrates for cathepsin G and elastase are 8.0-8.5 and 7.0-7.5, respectively. The isoelectric points for elastase and cathepsin G are 9.7-10.0 and greater than 10, respectively; the temperature optima--30-40 degrees C and 50-60 degrees C, respectively. The amino acid composition of the two enzymes from pig granulocytes revealed a high content of arginine and was similar to that of human granulocytes.
authors.forename: L N
authors.initials: LN
authors.lastname: Kraeva
authors.forename: V N
authors.initials: VN
authors.lastname: Kokriakov
authors.forename: I N
authors.initials: IN
authors.lastname: Chesnokov
authors.forename: M F
authors.initials: MF
authors.lastname: Iakovleva
authors.forename: S N
authors.initials: SN
authors.lastname: Lyzlova
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D056649:Cathepsin G; D002403:Cathepsins / Q000097:blood* / Q000302:isolation & purification; D002850:Chromatography, Gel; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000097:blood* / Q000302:isolation & purification; D012697:Serine Endopeptidases; D013552:Swine
chemical_list: D000596:Amino Acids; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase
vernacular_title: Nekotorye svoĭstva katepsina G i élastazy iz neĭtrofilov perifericheskoĭ krovi svin'i.