Continuous monitoring of the biliary excretion of a pulse of labelled compounds.
A simple method allowing the continuous monitoring of the biliary excretion of a labelled, pulse-injected compound in a single-pass perfused rat liver is described. The collection device is a mini-strip-chart recorder: bile is collected on its moving paper as a uniform trail that dries almost instantaneously. After rapid injection into the portal vein, the biliary excretion of a labelled compound can be assessed measuring the radioactivity on the paper by cutting and counting narrow strips of paper or by a radiochromatogram scanner. Experiments performed with [14C]-bilirubin, [3H]-taurocholate, and [14C]-nitrofurazone attest the validity of this technique, which can potentially improve the accuracy of hepatic transport studies in the isolated organ as well as in the intact animal.
Sorrentino
D
D
Department of Medicine, University of California, San Francisco.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002404:Catheterization; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013656:Taurocholic Acid / Q000378:metabolism; D013997:Time Factors
D013656:Taurocholic Acid