Ultrastructure of reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase-positive neurons in the cat cerebral cortex, amygdala and caudate nucleus.
The ultrastructure of reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase-positive neurons in cat cerebral cortex, amygdala and caudate nucleus was investigated by electron microscopy using a modified method applicable to aldehyde-fixed tissues. These NADPH diaphorase-positive neurons were morphologically similar to neurons immunohistochemically positive for somatostatin. They had large amounts of electron-dense formazan reaction products scattered through the whole cytoplasm but not in the mitochondria or nucleus. Similar electron-dense reaction products were visible in the dendrites of these neurons. The results indicate that NADPH diaphorase histochemistry is a useful method for the ultrastructural examination of particular groups of neurons.
Mizukawa
K
K
Department of Anatomy, Okayama University Medical School, Japan.
McGeer
P L
PL
Vincent
S R
SR
McGeer
E G
EG
D000679:Amygdala / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D002415:Cats; D002421:Caudate Nucleus / Q000201:enzymology* / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000201:enzymology* / Q000648:ultrastructure; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D009249:NADP / Q000032:analysis*
D009249:NADP