On the structure of alpha-crystallin: the minimum molecular weight.
alpha-crystallin can be isolated in two forms depending on the temperature at which the lens is extracted. At 4 degrees C, alpha c-crystallin is obtained while at 37 degrees C, a smaller molecule, alpha m-crystallin can be isolated. The apparent molecular weight of bovine foetal alpha m- and alpha c-crystallins were determined in 5 different ways using sedimentation velocity, sedimentation equilibrium and intensity fluctuation spectroscopy analyses and the experimentally determined diffusion coefficients, intrinsic viscosity and partial specific volume. Values ranged from 291,000 to 369,000 for alpha m and from 604,000 to 760,000 for alpha c due to the differential effects of the protein's polydispersity on the different methods. Subfractionation of the protein by gel filtration yielded much less polydisperse minimum species populations with molecular weights of 280,000 and 529,000 for alpha m and alpha c respectively. It was concluded that alpha-crystallin is probably synthesized as a symmetrical dodecamer and that the polydispersity of most preparations can be attributed to age-related modification in vivo as well as in vitro supra-aggregation due to variations in experimental conditions.
Thomson
J A
JA
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Augusteyn
R C
RC
D000818:Animals; D005591:Chemical Fractionation; D055598:Chemical Phenomena; D002621:Chemistry; D003459:Crystallins; D008970:Molecular Weight
D003459:Crystallins