An automated method for the kinetic measurement of ferroxidase activity.
The oxidase activity of caeruloplasmin towards Fe II (ferroxidase) appears to be of physiological significance both in iron metabolism and as a serum anti-oxidant. We have developed an automated assay for ferroxidase using a centrifugal analyser. The method is quick and precise, and is not subject to interference by haemolysis, jaundice or lipaemia. Ferroxidase activities correlate significantly with caeruloplasmin and copper concentrations. Normal reference ranges have been measured.
Winkles
J
J
Department of Biochemistry, Selly Oak Hospital, Birmingham, UK.
Jones
A F
AF
Winyard
P
P
Blake
D R
DR
Lunec
J
J
D000328:Adult; D001322:Autoanalysis; D002570:Ceruloplasmin / Q000378:metabolism*; D003300:Copper / Q000097:blood; D005296:Ferrous Compounds / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006949:Hyperlipidemias / Q000097:blood / Q000201:enzymology; D007202:Indicators and Reagents; D007565:Jaundice / Q000097:blood; D007700:Kinetics; D012016:Reference Values
D005296:Ferrous Compounds; D007202:Indicators and Reagents; D003300:Copper; D002570:Ceruloplasmin