Determination of reserpine and hydrochlorothiazide in commercial tablets by liquid chromatography with fluorescence and UV absorption detectors in series.
A procedure is presented for the determination of reserpine and hydrochlorothiazide in commercial tablets by liquid chromatography (LC). Reference and sample solutions are prepared in methanol. For LC, a normal phase column is used, methanol is the eluting solvent, and 2 detectors are arranged in series. A fluorescence detector set at an excitation wavelength of 280 nm and emission wavelength of 360 nm quantitates reserpine, and a UV absorption detector set at 345 nm determines hydrochlorothiazide. Several synthetic mixtures containing the 2 ingredients in the amounts approximately present in commercial tablets were analyzed by the proposed method. Two samples of commercial tablets were also analyzed; for each sample, 5 determinations were made on a ground composite of 20 tablets; 10 individual tablets were also analyzed. For comparison, some of the solutions were analyzed for each ingredient by an alternative procedure.
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Food and Drug Administration, Philadelphia, PA 19106.
D002853:Chromatography, Liquid; D006852:Hydrochlorothiazide / Q000032:analysis*; D007202:Indicators and Reagents; D012110:Reserpine / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets / Q000032:analysis*
D007202:Indicators and Reagents; D013607:Tablets; D006852:Hydrochlorothiazide; D012110:Reserpine