title: Erythroid-specific gene chromatin has an altered association with linker histones.
abstract: The chromatin of several genes was assayed for sensitivity to DNAase I and for solubility as polynucleosomes in 0.15 M NaCl. The degree of solubility of chromatin fragments as polynucleosomes in 0.15 M NaCl correlates well with the sensitivity to DNAase I for several genes. Chromatin of repressed, housekeeping and erythroid-specific genes can be distinguished as distinct groups by the degree to which they display these properties. NaCl precipitation of chromatin fragments stripped and then reconstituted with varying quantities of H1 and H5 (linker) histones indicate that the polynucleosomes of erythroid-specific genes have altered interaction with these histones. Linker histones interacted with bulk chromatin and in the chromatin of the repressed ovalbumin and vitellogenin genes to form salt precipitable structures. Chromatin of erythroid-specific genes (histone H5 and beta-globin) as well as that of the histone H2A.F gene was resistant to linker histone induced precipitation.
authors.affiliation: Department of Biochemistry, University of Manitoba, Winnipeg, Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Ridsdale
authors.forename: J B
authors.initials: JB
authors.lastname: Rattner
authors.forename: J R
authors.initials: JR
authors.lastname: Davie
mesh_terms: D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000187:drug effects*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004996:Ethidium / Q000494:pharmacology; D006657:Histones / Q000378:metabolism*; D008854:Microscopy, Electron; D009707:Nucleosomes / Q000032:analysis; D009994:Osmolar Concentration; D012995:Solubility
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D003850:Deoxyribonuclease I; D004996:Ethidium