Autologous red cell agglutination assay for HIV-1 antibodies: simplified test with whole blood.
An antibody detection procedure based on agglutination of autologous red cells has been developed for samples of whole blood. A nonagglutinating monoclonal antibody to human red blood cells conjugated to a synthetic peptide antigen (in this case residues 579 to 601 of the HIV-1 envelope precursor, Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp- Gly-Cys - Ser-Gly-Lys) permitted the detection of antibodies to the human immunodeficiency virus type 1 (HIV-1) in 10 microliters of whole blood within 2 minutes. Agglutination was specifically inhibited by addition of synthetic peptide antigen but not by unrelated peptides. The frequency of false positive results was 0.1% with HIV-1 seronegative blood donors (n = 874). The false negative results were approximately 1% (n = 81). The autologous red cell agglutination test is potentially suitable for simple, rapid, qualitative screening for antibodies to a variety of antigens of medical and veterinary diagnostic significance.
Kemp
B E
BE
Department of Medicine, University of Melbourne, Repatriation General Hospital, Victoria, Australia.
Rylatt
D B
DB
Bundesen
P G
PG
Doherty
R R
RR
McPhee
D A
DA
Stapleton
D
D
Cottis
L E
LE
Wilson
K
K
John
M A
MA
Khan
J M
JM
D000372:Agglutination Tests; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D006023:Glycoproteins / Q000276:immunology; D006678:HIV / Q000276:immunology*; D006679:HIV Seropositivity / Q000175:diagnosis*; D006801:Humans; D009842:Oligopeptides / Q000276:immunology; D012191:Retroviridae Proteins / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology
D000914:Antibodies, Viral; D006023:Glycoproteins; D009842:Oligopeptides; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins