title: A novel ganglioside in dogfish brain. Occurrence of a trisialoganglioside with a sialic acid linked to N-acetylgalactosamine.
abstract: A novel trisialoganglioside has been isolated from dogfish (Squalus acanthias) brain. From the results of gas chromatography-mass spectrometric analysis of methylated sugars, enzymatic hydrolysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance analysis, its structure was concluded to be (formula; see text) This is the first report of the occurrence of a ganglioside with 3 sialic acid residues separately linked to its gangliotetraosyl backbone.
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
authors.forename: Y
authors.initials: Y
authors.lastname: Tamai
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D004284:Dogfish / Q000378:metabolism*; D005688:Galactosamine / Q000031:analogs & derivatives*; D005732:Gangliosides / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000378:metabolism; D009522:Newcastle disease virus / Q000201:enzymology; D012754:Sharks / Q000378:metabolism*; D012794:Sialic Acids / Q000032:analysis*
chemical_list: D005732:Gangliosides; D012794:Sialic Acids; C016806:trisialoganglioside; D005688:Galactosamine; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D000116:Acetylgalactosamine