Diguanosine 5',5'''-P1,P4-tetraphosphate and other purine nucleotides inhibit endoribonuclease VI from Artemia.
The activity of the endoribonuclease VI from Artemia is sensitive to several purine nucleotides. The enzyme is non-competitively inhibited by diguanosine tetraphosphate (Ki = 75 microM), a nucleotide abundant in Artemia encysted gastrulae and located in the same particulate fraction as the gastrular ribonuclease. Diguanosine triphosphate and diadenosine tetraphosphate are less efficient inhibitors (Ki congruent to 200 microM). The ribonuclease is non-competitively inhibited by 5'-AMP (Ki = 10 microM) and 5'-GMP (Ki = 50 microM) but is insensitive to the corresponding 5'-phosphates of cytosine and uridine. Other purine mononucleotides inhibit the enzyme activity less efficiently. The modulation of the enzyme activity by these nucleotides is discussed in relation with the changes in ribonuclease activity during early development of Artemia.
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Instituto de Investigaciones Biomédicas del CSIC, Facultad de Medicina, UAM, Madrid, Spain.
Heredia
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D000818:Animals; D001156:Artemia / Q000201:enzymology* / Q000254:growth & development; D015226:Dinucleoside Phosphates; D004722:Endoribonucleases / Q000037:antagonists & inhibitors*; D006150:Guanine Nucleotides / Q000494:pharmacology*; D006159:Guanosine Tetraphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D007700:Kinetics; D011685:Purine Nucleotides / Q000494:pharmacology; D013329:Structure-Activity Relationship
D015226:Dinucleoside Phosphates; D006150:Guanine Nucleotides; D011685:Purine Nucleotides; D006159:Guanosine Tetraphosphate; C015138:diguanosine tetraphosphate; D004722:Endoribonucleases; C036214:endoribonuclease VI, Artemia