title: Effects of aging and hypertension on endothelium-dependent vascular relaxation in rat carotid artery.
abstract: We evaluated the effects of aging and hypertension on endothelium-dependent relaxation of rat common carotid arteries using 14-week-old (young) and 11-month-old (old) Wistar-Kyoto rats (WKY) and age-matched spontaneously hypertensive rats (SHR). Isometric tension of common carotid artery ring segments was measured. With a resting tension of 2.0 g determined from the baseline tension-contraction curves, precontraction was induced by 10(-5) M 5-hydroxytryptamine and endothelium-dependent relaxation was measured by application of either acetylcholine or adenosine 5'-triphosphate (ATP). Mean arterial blood pressure was 73.1 +/- 3.0 mm Hg in WKY and 110.0 +/- 3.1 mm Hg in SHR. These baseline values were significantly different. Acetylcholine-induced maximal relaxations were 70.1 +/- 2.6% of the 5-hydroxytryptamine-induced contraction in young WKY, 45.6 +/- 2.1% in old WKY, 35.1 +/- 1.8% in young SHR, and 21.4 +/- 2.5% in old SHR. On the other hand, ATP-induced relaxations were 52.0 +/- 3.2%, 35.7 +/- 3.8%, 21.7 +/- 3.5%, and 17.0 +/- 1.8% in the groups, respectively. Acetylcholine-induced relaxations were significantly different between WKY and SHR, young and old, independently. On the other hand, ATP-induced relaxations were also significantly different between young and old WKY, although no significant difference was observed between young and old SHR. The fact that endothelium-dependent relaxation of a cephalic artery is impaired in old rats and in hypertensive rats suggests that aging and hypertension are risk factors that may augment the disturbance of the cerebral circulation in pathologic conditions.
authors.affiliation: Department of Neurosurgery, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: K
authors.initials: K
authors.lastname: Hongo
authors.forename: T
authors.initials: T
authors.lastname: Nakagomi
authors.forename: N F
authors.initials: NF
authors.lastname: Kassell
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Lehman
authors.forename: D G
authors.initials: DG
authors.lastname: Vollmer
authors.forename: T
authors.initials: T
authors.lastname: Tsukahara
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: J
authors.initials: J
authors.lastname: Torner
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects / Q000503:physiopathology*; D002560:Cerebrovascular Circulation; D004727:Endothelium / Q000502:physiology; D006973:Hypertension / Q000503:physiopathology*; D007537:Isometric Contraction / Q000187:drug effects; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012307:Risk Factors; D012701:Serotonin / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects
chemical_list: D012701:Serotonin; D000255:Adenosine Triphosphate; D000109:Acetylcholine