title: Skin lipid content during early fetal development.
abstract: Although little is known about changes in the lipid composition of the skin during fetal development, information regarding the developmental sequence of fetal skin lipid content could be important for understanding the emergence of epidermal barrier function, as well as providing baseline criteria for prenatal diagnosis of certain inherited disorders of cornification. In these studies, epidermis was separated from dermis in fetal skin samples ranging from 50 to 140 d, estimated gestational ages (EGA), and its lipid composition was analyzed by quartz rod microchromatography/flame-ionization and thin layer chromatography. Lipid biochemical data were correlated with developmental milestones observed by electron microscopy (morphologic studies). The lipid composition of epidermal and dermal fractions from skin samples between 50 and 110 d EGA was similar, with both tissues exhibiting a predominance of free sterols and phospholipids. After 110 d EGA dermis became enriched in triglycerides, corresponding to the progressive development of adipocytes after this time. EGA epidermis after 110 d was enriched not only in triglycerides, but also sterol esters. Moreover, ceramides and glycosphingolipids also became increasingly prominent, changes that were greatest in epidermis from older fetuses and from cephalad regions. These changes in epidermal lipid composition corresponded morphologically to the progressive emergence of both folliculocentric epidermal cornification and sebaceous gland development.
authors.affiliation: Dermatology Service, Veterans Administration Medical Center, San Francisco, California 94121.
authors.forename: M L
authors.initials: ML
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Hincenbergs
authors.forename: K A
authors.initials: KA
authors.lastname: Holbrook
mesh_terms: D002784:Cholesterol / Q000032:analysis; D002855:Chromatography, Thin Layer; D005314:Embryonic and Fetal Development; D005260:Female; D005865:Gestational Age; D006028:Glycosphingolipids / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D012867:Skin / Q000032:analysis*; D013261:Sterols / Q000032:analysis
chemical_list: D006028:Glycosphingolipids; D008055:Lipids; D013261:Sterols; D002784:Cholesterol