Growth-stimulating effect of ferric citrate on hybridoma cells: characterization and relation to transferrin function.
The growth of mouse hybridomas in the defined medium with ferric citrate at the optimum growth-stimulating concentration of 500 microM in place of transferrin is fully comparable with the growth in the medium with transferrin at the optimum concentration of 5 micrograms/ml. Experiments with monoclonal antibody to mouse transferrin receptor demonstrate that the growth-stimulating effect of ferric citrate is not based on the action of residual transferrin or the action of transferrin secreted by hybridoma cells. Such growth-stimulating effect is specific for iron compounds while other metal trace elements are ineffective. Hybridoma cells can absorb iron from medium containing iron salt without the presence of transferrin.
Kovár
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
D000818:Animals; D002417:Cattle; D005290:Ferric Compounds / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D006825:Hybridomas / Q000187:drug effects*; D051379:Mice; D013045:Species Specificity; D013552:Swine; D014168:Transferrin / Q000502:physiology*
D005290:Ferric Compounds; D006133:Growth Substances; D014168:Transferrin; C025314:ferric citrate