title: Effect of parathion and methylparathion on protein content of chicken embryo muscle in vivo.
abstract: Chicken eggs were treated with 0.4 per cent solutions of parathion or methylparathion for four or eight days, and the two-dimensional gel electrophoretic protein pattern of cervical muscles of eighteen days old embryos was analyzed. Both compounds significantly decreased the content of alpha-actinin, alpha-tubulin and beta-tubulin after four days treatment, and, in addition, that of three other related proteins (gamma-proteins) after eight days treatment. Under in vitro phosphorylating conditions, both methylparathion and parathion specifically inhibited the phosphorylation of one isoform of beta-tubulin. Data suggest that the muscle-damaging effects of organophosphorous insecticides, such as parathion and its derivates, may be related to the decrease of tissue content of certain cytoskeletal proteins.
authors.affiliation: Institute of Plant Protection, University of Agricultural Sciences, Keszthely, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Deli
authors.forename: Z
authors.initials: Z
authors.lastname: Kiss
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003598:Cytoskeletal Proteins / Q000378:metabolism; D007526:Isoelectric Point; D008743:Methyl Parathion / Q000494:pharmacology*; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects / Q000196:embryology*; D010278:Parathion / Q000031:analogs & derivatives* / Q000494:pharmacology*; D010449:Peptide Mapping; D010750:Phosphoproteins / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D009124:Muscle Proteins; D010750:Phosphoproteins; D008743:Methyl Parathion; D010278:Parathion