Molecular and cytogenetic evidence for the location of Tdy and Hya on the mouse Y chromosome short arm.
Using a combination of in situ mapping and DNA analysis with recombinant DNA probes specific for the Sxr region of the mouse Y chromosome, we show that both the gene(s) controlling sex determination and the expression of the male-specific antigen H-Y (Tdy and Hya, respectively) are located on the minute short arm of the mouse Y chromosome. We demonstrate that the H-Y- variant of Sxr (Sxr') arose by a partial deletion within the Sxr region. Also, we show that intrachromosomal recombination between the Y short arm and Sxr' can sometimes occur during male meiosis, restoring the deleted DNA sequences and resulting in an H-Y+ mouse (male 719 in this paper). Based on these results, we propose a model for the generation of the original Sxr region and the Sxr' and Sxr719 variants.
Roberts
C
C
Unité d'Immunogénétique Humaine, Institut National de la Santé, Institut Pasteur, Paris, France.
Weith
A
A
Passage
E
E
Michot
J L
JL
Mattei
M G
MG
Bishop
C E
CE
D000818:Animals; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000378:metabolism; D008099:Liver / Q000502:physiology; D008297:Male; D051379:Mice / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012732:Sex Determination Analysis; D014998:Y Chromosome
D004274:DNA, Recombinant; D004247:DNA