title: Removal of contaminating nucleoside diphosphates from commercial preparations of uridine diphosphoglucose.
abstract: Nucleoside diphosphate contamination in commercial preparations of UDP-Glc poses potential problems in activity assays for enzymes that use this substrate. For removing these contaminants, we report a simple, inexpensive, and rapid method that obviates the need for uncertain corrections in assay calculations.
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee 32306-3050.
authors.forename: W H
authors.initials: WH
authors.lastname: Outlaw
authors.forename: D R
authors.initials: DR
authors.lastname: Hite
authors.forename: G B
authors.initials: GB
authors.lastname: Fiore
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D009702:Nucleoside Diphosphate Sugars / Q000302:isolation & purification*; D014532:Uridine Diphosphate Glucose / Q000032:analysis*; D014539:Uridine Diphosphate Sugars / Q000032:analysis*
chemical_list: D009702:Nucleoside Diphosphate Sugars; D014539:Uridine Diphosphate Sugars; D014532:Uridine Diphosphate Glucose