Receptor-mediated uptake of starch and mannan microparticles by macrophages: relative contribution of receptors for complement, immunoglobulins and carbohydrates.
The contribution of different macrophage receptors to the uptake of polyacryl starch and polyacryl mannan microparticles by macrophages was studied. Both types of microparticle were taken up to a larger and similar extent when they had been pre-incubated with serum, indicating the importance of serum factors in the uptake process. These results were supported by organ distribution studies in mice, showing that the two microparticles were rapidly targeted to the liver and spleen after i.v. injection. The carbohydrate-specific mannose/fucose receptor was found to contribute significantly to the uptake of non-opsonized but not opsonized mannan microparticles. The two different microparticles were found to activate the alternative complement pathway and to adsorb immunoglobulin G, human serum albumin and fibronectin to their surface. Inhibition experiments provided evidence for the involvement of a complement receptor (CR3) and an Fc-receptor (FCR) for IgG in the uptake of opsonized microparticles.
Artursson
P
P
Department of Drugs, National Board of Health and Welfare, Uppsala, Sweden.
Johansson
D
D
Sjöholm
I
I
D000818:Animals; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D007700:Kinetics; D037181:Lectins, C-Type; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008351:Mannans / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D011956:Receptors, Cell Surface; D011951:Receptors, Complement / Q000378:metabolism*; D011961:Receptors, Fc / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D013213:Starch / Q000378:metabolism* / Q000493:pharmacokinetics
D002250:Carbon Radioisotopes; D037181:Lectins, C-Type; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011951:Receptors, Complement; D011961:Receptors, Fc; D011971:Receptors, Immunologic; D013213:Starch; D008358:Mannose