title: Functional properties of proteins immobilized on albumin microspheres.
abstract: Bovine serum albumin (BSA) microspheres with an average diameter of 12.5 micron were prepared by crosslinking of BSA molecules with glutaraldehyde in the presence of polymethylmethacrylate dissolved in chloroform-toluene. Trypsin and anti-human IgG antibody were immobilized onto their surfaces by the glutaraldehyde-activation method. The catalytic activity and storage stability of the immobilized trypsin were satisfactorily high. The enzyme immunoassay (EIA) method using BSA-microspheres as a solid phase has a high sensitivity (the minimum concentration of detectable antigen in the sample: 0.2 ng/ml) and a wide concentration range (final concentration 0.027-3000 ng/ml) for the detection of human IgG.
authors.affiliation: Department of Polymer Chemistry, Kyoto University, Japan.
authors.forename: C H
authors.initials: CH
authors.lastname: Yan
authors.forename: H
authors.initials: H
authors.lastname: Kitano
authors.forename: N
authors.initials: N
authors.lastname: Ise
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007700:Kinetics; D008863:Microspheres; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D014357:Trypsin / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D014357:Trypsin