Simplified culture procedure for large-scale screening for Chlamydia trachomatis infections.
A method that uses a 48-well tissue culture cluster tray system for the isolation of Chlamydia trachomatis is described. The cluster tray system was as sensitive (100%) as and more time efficient than the conventional cover slip method, thereby being considerably cost saving. With both culture methods, the prevalence rates of genital carriage of C. trachomatis in women attending clinics for legal abortion and for cervical dysplasia were 5% (31 of 641 patients) and 2% (3 of 148 patients), respectively.
Lees
M I
MI
Department of Pathology, Royal Women's Hospital, Melbourne, Victoria, Australia.
Newnan
D M
DM
Garland
S M
SM
D000293:Adolescent; D000328:Adult; D002353:Carrier State / Q000175:diagnosis* / Q000453:epidemiology; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000453:epidemiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005260:Female; D005831:Genital Diseases, Female / Q000175:diagnosis* / Q000453:epidemiology; D006367:HeLa Cells; D006801:Humans; D008875:Middle Aged; D011237:Predictive Value of Tests; D011247:Pregnancy; D014739:Victoria