The inhibitory effect of serum on cell attachment can be prevented by cobalt-protoporphyrin IX.
The rate of attachment of chick embryo fibroblasts (CEF) incubated for 1 h at pH 6 in the absence of serum, was equal to about 90% with 2% of spreading cells. The addition of 1% horse serum (HS) decreased to about 30% the rate of attached cells but increased to about 30% the proportion of spreading cells. We found that 3 microM cobalt-protoporphyrin IX (CoPP) added for 3 min before the addition of 1% HS increased both the rate of cell attachment (about 60%) and the proportion of cell spreading (about 50%).
Verger
C
C
ER 303 C.N.R.S., I.R.S.C., Villejuif, France.
Imbenotte
J
J
D000818:Animals; D001769:Blood; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D005347:Fibroblasts; D011166:Porphyrins / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology*
D011166:Porphyrins; D011524:Protoporphyrins; C007095:cobaltiprotoporphyrin