title: Several intermediate forms in the processing of rat lutropin subunits as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis.
abstract: To study the biosynthesis in situ of lutropin (LH) subunits, anterior pituitary cells in culture were employed. The cells were incubated in the presence of [35S]methionine. Labelled polypeptides, immunologically related to alpha and LH beta subunits, were isolated by specific immunoprecipitation, then analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and revealed by fluorography. Electrophoretic analysis of immunoprecipitates is a crucial step in this methodology. It permits simultaneous purification, characterization and accurate quantitation of the radioactivity specifically incorporated into LH subunits. Also, using SDS-PAGE, it is possible to isolate and identify the different processed forms of LH subunits.
authors.affiliation: Laboratoire des Hormones Polypeptidiques, CNRS, Gif-sur-Yvette, France.
authors.forename: A
authors.initials: A
authors.lastname: Starzec
authors.forename: R
authors.initials: R
authors.lastname: Counis
authors.forename: M
authors.initials: M
authors.lastname: Jutisz
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D007986:Luteinizing Hormone / Q000096:biosynthesis / Q000302:isolation & purification*; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012967:Sodium Dodecyl Sulfate; D014415:Tunicamycin / Q000302:isolation & purification
chemical_list: D014415:Tunicamycin; D012967:Sodium Dodecyl Sulfate; D007986:Luteinizing Hormone