Nucleotide sequence predicts circularity and self-cleavage of 300-ribonucleotide satellite of arabis mosaic virus.
The nucleotide sequence of the satellite of arabis mosaic virus was determined using the satellite RNA encapsidated in virions. The 300-nucleotide long sequence showed extensive homology (50%) with that of the 359-nucleotide satellite RNA of tobacco ringspot virus, which occurs both in a linear and a circular form. This homology also revealed the presence of conceived sequences believed to mediate self-cleavage of the latter as well as other viral satellite RNAs. A circular form of the arabis mosaic virus satellite can be isolated from infected tissues and partially converts to the linear form upon elution from denaturing gels.
Kaper
J M
JM
Microbiology and Plant Pathology Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Tousignant
M E
ME
Steger
G
G
D001483:Base Sequence; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000235:genetics*; D014771:Virion / Q000235:genetics
D012367:RNA, Viral