Mevalonate 5-pyrophosphate decarboxylase in isolated villus and crypt cells of chick intestine.
Mevalonate 5-pyrophosphate decarboxylase was studied in isolated enterocytes obtained from duodenal, jejunal and ileal villi and crypts. In our assay conditions, decarboxylase activity was linear for 60 min and up to 0.3 mg of protein. The subcellular location of decarboxylase in chick enterocytes was investigated. About 94% of the total activity was recovered in the cytosol. The distribution of enzyme activity in epithelial cells also was studied. Maximal specific activity was found in cell fractions from jejunum followed by ileum and duodenum. About 80% of total activity was recovered in the villus cells, indicating an active role of these cells in cholesterogenesis. Ileal cells showed the highest cholesterol content. In all the intestinal epithelial cells assayed, free cholesterol represented about 95% of the total cholesterol.
Iglesias
J
J
Department of Biochemistry and Molecular Biology, University of Granada, Spain.
Gonzalez-Pacanowska
D
D
Caamaño
G
G
Garcia-Peregrin
E
E
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002645:Chickens; D002784:Cholesterol / Q000032:analysis; D007422:Intestines / Q000166:cytology / Q000201:enzymology*; D008297:Male
D002784:Cholesterol; D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase