1H- and 13C-NMR spectroscopic study of the metabolites in young adult Angiostrongylus cantonensis maintained in vitro.
1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy were used to study metabolites excreted by young adult Angiostrongylus cantonensis maintained aerobically in the presence of D-[13C6]glucose. End-products of glucose metabolism identified and quantitated by means of 1H-NMR were lactate, acetate and alanine, in the molar ratio of 1:0.13:0.05 for males and 1:0.07:0.04 for females. 13C-NMR analyses proved that all the three products originated from the glucose present in the medium.
Nishina
M
M
Department of Parasitology, Saitama Medical School, Japan.
Hori
E
E
Matsushita
K
K
Takahashi
M
M
Kato
K
K
Ohsaka
A
A
D000085:Acetates / Q000032:analysis*; D000409:Alanine / Q000032:analysis*; D000801:Angiostrongylus / Q000378:metabolism*; D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008681:Metastrongyloidea / Q000378:metabolism*
D000085:Acetates; D007773:Lactates; D005947:Glucose; D000409:Alanine