Quantitation of polyamines using thin-layer chromatography and image analysis.
Efficient separation of dansylated polyamines can be achieved by thin-layer chromatography (TLC). Quantitation, however, can be laborious because it requires removal of the silica gel and the fluorescing derivative from the glass plates, elution in a suitable solvent, and estimation with a fluorescence spectrophotometer. We report here a relatively simple and rapid method for the quantitation of dansylated polyamines that employs an image analyzer without removal from the glass TLC plates.
Wettlaufer
S H
SH
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853.
Weinstein
L H
LH
D002855:Chromatography, Thin Layer; D010944:Plants / Q000032:analysis; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis
D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine