Deletion analysis of bacteriophage T4 tertiary origins. A promoter sequence is required for a rifampicin-resistant replication origin.
The DNA sequence requirements of two T4 tertiary replication origins have been characterized by a deletion analysis of origin-containing plasmids. Maximal replication of each origin-containing plasmid required both an intact gpmotA-dependent middle-mode promoter sequence and approximately 50 base pairs of the downstream region. In contrast, gpmotA-dependent transcription from the origin promoter was found to be independent of the downstream region. The requirement for a promoter element within the tertiary origins is striking, particularly since the replication of tertiary origin-containing plasmids is resistant to the RNA polymerase inhibitor rifampicin.
Menkens
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AE
Department of Microbiology and Immunology, Duke University Medical Center, Durham 27710.
Kreuzer
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KN
D001483:Base Sequence; D002872:Chromosome Deletion; D004352:Drug Resistance, Microbial; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012293:Rifampin / Q000494:pharmacology*; D013604:T-Phages / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic; D014779:Virus Replication / Q000187:drug effects*
D012293:Rifampin