title: Platelet-activating factor induces eosinophil peroxidase release from purified human eosinophils.
abstract: The degranulation response of purified human eosinophils to platelet-activating factor (PAF) has been studied. PAF induced release of eosinophil peroxidase (EPO) and beta-glucuronidase from highly purified human eosinophils with an EC50 of 0.9 nM. The order of release was comparable with that induced by phorbol myristate acetate (PMA). The new specific PAF antagonist 3-[4-(2-chlorophenyl)-9-methyl-H-thieno[3,2-f] [1,2,4]triazolo-[4,3a][1,4]-diazepin-2-yl](4-morpholinyl)- 1-propane-one (WEB 2086) inhibited the PAF-induced enzyme release by human eosinophils in a dose-dependent manner. The viability of eosinophils were unaffected both by PAF and WEB 2086. The results suggest that PAF may amplify allergic and inflammatory reactions by release of preformed proteins from eosinophil granules.
authors.affiliation: Department of Thoracic Medicine, Brompton Hospital, London.
authors.forename: C
authors.initials: C
authors.lastname: Kroegel
authors.forename: T
authors.initials: T
authors.lastname: Yukawa
authors.forename: G
authors.initials: G
authors.lastname: Dent
authors.forename: P
authors.initials: P
authors.lastname: Chanez
authors.forename: K F
authors.initials: KF
authors.lastname: Chung
authors.forename: P J
authors.initials: PJ
authors.lastname: Barnes
mesh_terms: D001381:Azepines / Q000494:pharmacology; D047209:Eosinophil Peroxidase; D004804:Eosinophils / Q000201:enzymology*; D006801:Humans; D010544:Peroxidases / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D014227:Triazines / Q000494:pharmacology; D014230:Triazoles
chemical_list: D001381:Azepines; D010972:Platelet Activating Factor; D014227:Triazines; D014230:Triazoles; C052518:WEB 2086; D047209:Eosinophil Peroxidase; D010544:Peroxidases