Estrogen hydroxylase activity in the human placenta at term.
Placental estrogen hydroxylase (EH) enzyme activity was measured at term using the catechol-O-methyl transferase coupled method in normal and high risk conditions. The identity and ratio of products formed during incubation of microsomes as analysed by high performance liquid chromatography in chronic hypertension, toxemia and diabetes mellitus was not different from controls. The mean enzymatic activity was also not different among the conditions studied as expressed mean +/- SE pmol/min/mg, protein: chronic hypertension (7.8 +/- 1), toxemia (8 +/- 1.6), diabetes mellitus (6.1 +/- 0.9) and controls (8.3 +/- 1.5). The cofactor dependence of EH was studied showing that NADPH is a better substrate for the enzyme than NADH.
Barnea
E R
ER
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT.
MacLusky
N J
NJ
Purdy
R
R
Naftolin
F
F
D002394:Catechol O-Methyltransferase / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D009249:NADP / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D011225:Pre-Eclampsia / Q000201:enzymology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000201:enzymology*; D011249:Pregnancy Complications, Cardiovascular / Q000201:enzymology; D011254:Pregnancy in Diabetics / Q000201:enzymology; D013250:Steroid Hydroxylases / Q000378:metabolism*
D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D019363:Cytochrome P-450 CYP1A1; C020120:estrogen 2-hydroxylase; D002394:Catechol O-Methyltransferase