[Cultivated aortic cells--demonstration of muscarinic receptors and reaction behavior in the presence of acetylcholine].
Using the muscarinic ligand 3H-quinuclidinyl benzilate the presence of muscarinic receptors on cells isolated from aortas of newborn rats and cultivated for 6 d could be shown. The number of receptors per cell was estimated with 17,400 on cells of confluent and with 87,100 on cells of nonconfluent cultures. Incubation of cells with increasing concentrations of acetylcholine in the range of 10(-13)-10(-7) mol.l-1 resulted in the reaction of growing numbers of cells following the all-or-nothing-law. Physiological concentrations of acetylcholine lead to contractions, showing that dilatations of blood vessels after application of acetylcholine in situ are caused by reaction products of endothelial cells, being absent in the cultures.
Mundt
S
S
Sektion Pharmazie der Ernst-Moritz-Arndt-Universität Greifswald.
Buthut
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U
Teuscher
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E
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001013:Aorta, Thoracic / Q000166:cytology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D008297:Male; D011813:Quinuclidinyl Benzilate; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000187:drug effects*
D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D000109:Acetylcholine
Kultivierte Aortenzellen--Nachweis muscarinerger Rezeptoren und Reaktionsverhalten in Gegenwart von Acetylcholin.