Interactions between ascorbic acid and linear and cyclized bile pigments. A thin-layer chromatographic study.
Physico-chemical interactions between ascorbic acid and the linear or cyclized bile pigment dimethyl esters were determined by thin-layer chromatography on silica gel. The interacting barrier technique allows a reactivity to be established that is a function of the degree of cyclization of the molecule. Thus, linear pigments of the biliverdin type are of low reactivity whereas the monocyclic neopterobilin phorcabilin shows a medium-strength interaction and isophorcabilin a strong interaction, the natural sarpedobilin being definitely captured by the ascorbic acid barrier on the silica gel layer. Spectrophotometric studies of the ascorbate-sarpedobilin interaction led to the conclusion that more than 2 mol of ascorbic acid are required per mole of pigment. The results are discussed in connection with the possible biochemical role of such bilins.
Barbier
M
M
Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France.
D001205:Ascorbic Acid / Q000032:analysis*; D001654:Bile Pigments / Q000032:analysis*; D002855:Chromatography, Thin Layer; D006801:Humans
D001654:Bile Pigments; D001205:Ascorbic Acid