Circular dichroism and fluorescence investigations on Vicia faba lectin-saccharide binding.
Vicia faba lectin contained 40-57% beta-conformation, 4-23% alpha-conformation along with random coil at pH 7.2 depending upon the analytical methods used. The percentage of beta-conformation increased with the addition of N-acetyl-D-glucosamine or methyl alpha-D-mannopyranoside. The structural transitions of V. faba lectin were affected by alkali at pH 9.6 and 10.6. Binding constants and free energy changes for the interaction between V. faba lectin and N-acetyl-D-glucosamine and methyl alpha-D-mannopyranoside were estimated at pH 7.2 using the c.d. and fluorescence methods.
Datta
P K
PK
Indian Institute of Chemical Biology, Jadavpur, Calcutta.
Basu
P S
PS
Datta
T K
TK
D000117:Acetylglucosamine / Q000378:metabolism*; D002942:Circular Dichroism; D007887:Fabaceae / Q000378:metabolism*; D005944:Glucosamine / Q000031:analogs & derivatives*; D007700:Kinetics; D037102:Lectins / Q000378:metabolism*; D046911:Macromolecular Substances; D008759:Methylglycosides / Q000378:metabolism*; D008766:Methylmannosides / Q000378:metabolism*; D037121:Plant Lectins; D010946:Plants, Medicinal; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D037102:Lectins; D046911:Macromolecular Substances; D008759:Methylglycosides; D008766:Methylmannosides; D037121:Plant Lectins; C008466:methylmannoside; D005944:Glucosamine; D000117:Acetylglucosamine