title: Fucose-containing oligosaccharides from human milk from a donor of blood group 0 Le(a) nonsecretor.
abstract: The neutral oligosaccharides from the milk of a single donor with blood group 0, Lewis(a+b-) nonsecretor were separated into 18 fractions essentially according to the number of carbohydrate constituents using gel permeation chromatography on Biogel P-4 and Fractogel TSK HW-40. Further separation was achieved by HPLC and by HPTLC after reduction and peracetylation. The fractions obtained were analysed by FAB mass spectrometry with and without derivatization and by one- and two-dimensional proton NMR. Besides the already described 3-fucosidolactose, fucopentaose II (2), fucopentaose III (6) and difucohexaose II (4) the following fucosylated oligosaccharides could be identified. Among the higher oligosaccharides a branched lacto-N-decaose (12) was obtained in pure form after removal of the fucose residues by mild acid treatment.
authors.affiliation: Institut für Physiologische Chemie der Universität Bonn.
authors.forename: R
authors.initials: R
authors.lastname: Bruntz
authors.forename: U
authors.initials: U
authors.lastname: Dabrowski
authors.forename: J
authors.initials: J
authors.lastname: Dabrowski
authors.forename: A
authors.initials: A
authors.lastname: Ebersold
authors.forename: J
authors.initials: J
authors.lastname: Peter-Katalinić
authors.forename: H
authors.initials: H
authors.lastname: Egge
mesh_terms: D000017:ABO Blood-Group System; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005643:Fucose / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D007983:Lewis Blood Group Antigens; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008895:Milk, Human / Q000032:analysis*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*
chemical_list: D000017:ABO Blood-Group System; D007202:Indicators and Reagents; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D005643:Fucose