Time dependence of whole blood aggregation in response to platelet activating factor (PAF).
The aggregation/adhesion response to platelet activating factor (PAF) was studied in diluted whole blood by impedance aggregometry. The extent of aggregation varied directly with the interval between blood sampling and aggregation measurement over the first 30 minutes from sampling, then remained stable for the next 60 minutes of observation. This is an effect opposite to that described for aggregation to PAF in platelet rich plasma which, however, cannot be studied soon after sampling. Time dependence of aggregation is important and comparative measurements should be made during the period of stable aggregability.
Taylor
R R
RR
Department of Medicine, University of Western Australia, Perth.
Strophair
J
J
Sturm
M
M
Vandongen
R
R
Beilin
L J
LJ
D006801:Humans; D066298:In Vitro Techniques; D010972:Platelet Activating Factor / Q000502:physiology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D012907:Smoking / Q000097:blood; D014914:Whole Blood Coagulation Time
D010972:Platelet Activating Factor