Purification and properties of formate dehydrogenase from Moraxella sp. strain C-1.
NAD+-dependent formate dehydrogenase was screened in various bacterial strains. Facultative methanol-utilizing bacteria isolated from soil samples, acclimated to a medium containing methanol and formate at pH 9.5, were classified as members of the genus Moraxella. From a crude extract of Moraxella sp. strain C-1, formate dehydrogenase was purified to homogeneity, as judged by disc gel electrophoresis. The enzyme has an isoelectric point of 3.9 and a molecular weight of approximately 98,000. The enzyme is composed of two identical subunits with molecular weights of about 48,000. The apparent Km values for sodium formate and NAD+ were calculated to be 13 mM and 0.068 mM, respectively.
Asano
Y
Y
Sagami Chemical Research Center, Kanagawa, Japan.
Sekigawa
T
T
Inukai
H
H
Nakazawa
A
A
D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005560:Formate Dehydrogenases / Q000032:analysis / Q000302:isolation & purification*; D005561:Formates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007700:Kinetics; D000432:Methanol / Q000378:metabolism; D008970:Molecular Weight; D009016:Moraxella / Q000201:enzymology* / Q000254:growth & development; D012988:Soil Microbiology; D013379:Substrate Specificity; D013696:Temperature
D003470:Culture Media; D005561:Formates; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases; D000432:Methanol