title: Absence of a characteristic cell wall lipopolysaccharide in the phototrophic bacterium Chloroflexus aurantiacus.
abstract: Two strains of the gliding phototrophic bacterium Chloroflexus aurantiacus were investigated for the presence of lipopolysaccharide (LPS). With both strains, all fractions of hot phenol-water extracts and the extracted cell residues from whole cells or cell homogenates were found to be free from characteristic LPS constituents, such as 3-hydroxy fatty acids, 2-keto-3-deoxyoctonate, heptoses, or O-chain sugars. Phenolchloroform-petroleum ether extracts were also free from precipitable LPS. A lipid A fraction could not be obtained, and there was no hint for glucosamine as a possible lipid A backbone amino sugar. Absence of LPS was confirmed by sodium deoxycholate gel electrophoresis.
authors.affiliation: Institut für Biologie II, Albert-Ludwigs-Universität, Freiburg im Breisgau, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Meissner
authors.forename: J H
authors.initials: JH
authors.lastname: Krauss
authors.forename: U J
authors.initials: UJ
authors.lastname: Jürgens
authors.forename: J
authors.initials: J
authors.lastname: Weckesser
mesh_terms: D001419:Bacteria / Q000032:analysis* / Q000648:ultrastructure; D002473:Cell Wall / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis*
chemical_list: D008070:Lipopolysaccharides