The structure of nephritogenoside. A nephritogenic glycopeptide with alpha-N-glycosidic linkage.
Nephritogenoside is a glycopeptide with the ability to induce chronic progressive glomerulonephritis (end stage kidney) in homologous animals, by a single footpad injection. This substance contains a novel carbohydrate-peptide linkage, i.e. the trisaccharide (3 glucose residues) chain is alpha-N-glycosidically linked to the polypeptide chain through the amido nitrogen of an asparagine residue at the N-terminal. It was found that the peptide portion of nephritogenoside is composed of twenty-one amino acids (Asn1-Pro-Leu-Phe-Gly5-Ile-Ala-Gly-Glu-Asp10-Gly-Pro-Thr-Gly-Pr o15-Ser-Gly-Ile- Val-Gly20-Gln21) and that the peptide portion has a repeated Gly-X-Y structure.
Shibata
S
S
Clinical Research Institute, National Medical Center, Tokyo, Japan.
Takeda
T
T
Natori
Y
Y
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis; D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D003094:Collagen; D006023:Glycoproteins / Q000032:analysis; D007678:Kidney Glomerulus / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D051381:Rats; D011952:Receptors, Concanavalin A; D012091:Repetitive Sequences, Nucleic Acid
D000596:Amino Acids; D006023:Glycoproteins; D011952:Receptors, Concanavalin A; C015382:nephritogenoside; D003094:Collagen