Benzamide-inhibitable alterations in spectral properties of chromatin accompany DNA repair in UVC-exposed L5178Y cells.
We examined the response of chromatin to increasing NaCl and MgCl2 concentrations in UVC-irradiated L5178Y (LY) R and S cells, using the spectral index method (Dixon and Burkholder 1985). We have found an alteration in chromatin properties 1 h after UVC-irradiation of repair proficient LY-S cells, but no change in repair deficient LY-R cells. The change was shown as lowered spectral index, indicating that at given Na+ and Mg++ concentrations (1 or 200 mM NaCl, 0 or 0.5 mM MgCl2) chromatin from UVC-irradiated LY-S cells was more compact than that from unirradiated ones. Benzamide treatment reversed the effect of UVC-irradiation in LY-S cells and did not change the response pattern of chromatin from LY-R cells or unirradiated LY-S cells.
Kleczkowska
H E
HE
Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland.
Buraczewska
I
I
Szumiel
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I
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002460:Cell Line; D002843:Chromatin / Q000187:drug effects*; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects*; D007940:Leukemia L5178 / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics*; D008274:Magnesium / Q000032:analysis; D051379:Mice; D012964:Sodium / Q000032:analysis; D014466:Ultraviolet Rays
D001549:Benzamides; D002843:Chromatin; C037689:benzamide; D004247:DNA; D012964:Sodium; D008274:Magnesium