Site-directed ELISA identifies a highly antigenic region of the simian immunodeficiency virus transmembrane glycoprotein.
The transmembrane glycoprotein (gp32) of the simian immunodeficiency virus (SIV) contains a highly antigenic region that includes amino acid residues 606-628. A synthetic peptide representing this region was highly immunoreactive with sera from SIV-infected primates in a site-directed enzyme-linked immunosorbent assay (ELISA). This reactivity extended across four primate species from three genera and identified infection with at least two distinct isolates of SIV. This site-directed ELISA represents a simple, accessible method with broad specificity for screening large numbers of primates for antibodies against SIV.
Johnson
P R
PR
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD.
Parks
D E
DE
Norrby
E
E
Lerner
R A
RA
Purcell
R H
RH
Chanock
R M
RM
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000382:microbiology*; D012190:Retroviridae / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000032:analysis*
D000956:Antigens, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins