title: Changes in adrenocortical monooxygenase activities in alloxan-diabetic rabbits.
abstract: Studies were carried out to determine if diabetes mellitus influenced the activities of adrenal steroidogenic enzymes. Adult male rabbits were made diabetic by an i.v. infusion of alloxan (100 mg/kg) and were killed 1 or 2 months later. Mitochondrial cytochrome P-450 concentrations were not affected by diabetes but steroid 11 beta-hydroxylase activity was greater in the diabetics than in controls after both 1 and 2 months. The type I spectral change produced by 11-deoxycorticosterone, the substrate for 11 beta-hydroxylation, was also greater in mitochondria from diabetics. By contrast, mitochondrial cholesterol side-chain cleavage activity was similar in controls and diabetics. Microsomal cytochrome P-450 concentrations were unaffected by diabetes but 21-hydroxylase activity was significantly lower in adrenal microsomes from diabetics than from controls. The results indicate that alloxan-induced diabetes alters adrenocortical steroid metabolism which may contribute to changes in the pattern of steroid secretion noted by other investigators.
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford 61107.
authors.forename: H D
authors.initials: HD
authors.lastname: Colby
authors.forename: L B
authors.initials: LB
authors.lastname: Lacagnin
authors.forename: P A
authors.initials: PA
authors.lastname: Longhurst
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D011817:Rabbits; D012016:Reference Values; D013250:Steroid Hydroxylases / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D002786:Cholesterol Side-Chain Cleavage Enzyme