title: Selenium and glutathione peroxidase in seminal plasma of men and bulls.
abstract: High levels of selenium and glutathione peroxidase (GSH-Px) were found in bull seminal plasma but low concentrations in human seminal plasma. In man the seminal plasma selenium was associated with two macromolecules separable by gel filtration, but no GSH-Px was found in the same fractions. Selenium in bull seminal plasma was associated with two proteins, which could be separated by gel filtration and anion exchange chromatography. Both macromolecules coeluted with GSH-Px activity and had identical optima at pH 7.0. Their responses to thermal treatment, however, differed. Seminal vesicle secretory fluid in the bull contained both these proteins, while the larger molecule was also found in fractionations of ampulla, prostate and Cowper's glands. The larger enzyme form is evidently a tetramer of the smaller one. Both enzyme forms were extremely sensitive to heavy metals and some divalent metal ions. GSH caused an activation while other reducing agents were suppressive. Triton X-100 had no effect, while sodium deoxycholate was inhibitory. These properties are typical for a phospholipid hydroperoxide GSH-Px. It is concluded that this selenium-dependent enzyme may be important in the protection of bovine spermatozoa against damage caused by oxygen radicals, while in man such a mechanism is not functional.
authors.affiliation: Department of Chemistry, University of Kuopio, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Kantola
authors.forename: M
authors.initials: M
authors.lastname: Saaranen
authors.forename: T
authors.initials: T
authors.lastname: Vanha-Perttula
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005979:Glutathione Peroxidase / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008297:Male; D008970:Molecular Weight; D012643:Selenium / Q000032:analysis*; D012661:Semen / Q000032:analysis*; D012669:Seminal Vesicles / Q000201:enzymology
chemical_list: D005979:Glutathione Peroxidase; D012643:Selenium