title: The effect of fast protein liquid chromatography (FPLC) cationic exchange purification on the crystallization of a monoclonal Fab fragment.
abstract: Fast protein liquid chromatography cationic exchange purification to homogeneity of a monoclonal Fab fragment has resulted in an improvement in the quality of crystals for X-ray diffraction studies. This improvement is displayed in a well-defined crystal morphology, reproducibility of crystal growth, and increased resolution of diffraction data.
authors.affiliation: St. Vincent's Institute of Medical Research, Melbourne, Victoria, Australia.
authors.forename: J D
authors.initials: JD
authors.lastname: Orbell
authors.forename: L W
authors.initials: LW
authors.lastname: Guddat
authors.forename: K J
authors.initials: KJ
authors.lastname: Machin
authors.forename: N W
authors.initials: NW
authors.lastname: Isaacs
mesh_terms: D002852:Chromatography, Ion Exchange; D003460:Crystallization; D007140:Immunoglobulin Fab Fragments / Q000032:analysis*; D014961:X-Ray Diffraction
chemical_list: D007140:Immunoglobulin Fab Fragments