The use of [185W] thiotungstates for tracer studies in secondary hypocuprosis in cattle.
[185W] thiotungstates were synthesized in-vitro and purified by techniques developed for thiomolybdates. [185W] trithiotungstate (15 mg W) injected intravenously into cattle was mainly associated with albumin albumin. The presence of the compound in plasma when injected in larger amounts (0.2 to 1 gW) caused an increase in albumin-bound copper and transiently depressed ceruloplasmin diamine oxidase activity. It is suggested that the long-lived, less costly 185W could prove a convenient alternative to 99Mo for tracer studies with labelled thio-compounds in animals.
Mason
J
J
Biochemistry Department, Trinity College, Dublin.
Smith
A
A
Wang
Z Y
ZY
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D003300:Copper / Q000172:deficiency* / Q000378:metabolism; D008982:Molybdenum / Q000378:metabolism; D011868:Radioisotopes; D014414:Tungsten; D017973:Tungsten Compounds
D011868:Radioisotopes; D017973:Tungsten Compounds; C056542:trithiotungstic acid; C034540:trithiomolybdic acid; D003300:Copper; D008982:Molybdenum; D014414:Tungsten