title: Transcription of T cell receptor beta-chain genes is controlled by a downstream regulatory element.
abstract: To characterize cis-acting elements controlling the expression of T cell receptor beta-chains we generated a number of transgenic mouse lines harboring a rearranged T cell receptor beta-chain with different extensions of 5' and 3' flanking sequences. Transcriptional analysis of transgenic mice carrying these clones showed that sequences located downstream of the polyadenylation signal of the C beta 2 region are indispensable for expression in transgenic mice. The sequences conferring enhancer activity in this fragment were further defined by transient CAT assays. Strong enhancer activity was found to reside in a 550 bp fragment located 5 kb downstream from C beta 2. The nucleotide sequence of this fragment revealed a number of oligonucleotide motifs characteristic for enhancer elements.
authors.affiliation: Division of Molecular Genetics, the Netherlands Cancer Institute, Amsterdam.
authors.forename: P
authors.initials: P
authors.lastname: Krimpenfort
authors.forename: R
authors.initials: R
authors.lastname: de Jong
authors.forename: Y
authors.initials: Y
authors.lastname: Uematsu
authors.forename: Z
authors.initials: Z
authors.lastname: Dembic
authors.forename: S
authors.initials: S
authors.lastname: Ryser
authors.forename: H
authors.initials: H
authors.lastname: von Boehmer
authors.forename: M
authors.initials: M
authors.lastname: Steinmetz
authors.forename: A
authors.initials: A
authors.lastname: Berns
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011971:Receptors, Immunologic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D011971:Receptors, Immunologic; D004247:DNA