A transcription factor active on the epidermal growth factor receptor gene.
We have developed an in vitro transcription system for the epidermal growth factor receptor (EGFR) oncogene by using nuclear extracts of A431 human epidermoid carcinoma cells, which overproduce EGFR. We found that a nuclear factor, termed EGFR-specific transcription factor (ETF), specifically stimulated EGFR transcription by 5- to 10-fold. In this report, ETF, purified by using sequence-specific oligonucleotide affinity chromatography, is shown by renaturing material eluted from a NaDodSO4/polyacrylamide gel to be a protein with a molecular mass of 120 kDa. ETF binds to the promoter region, as measured by DNase I "footprinting" and gel-mobility-shift assays, and specifically stimulates the transcription of the EGFR gene in a reconstituted in vitro transcription system. These results suggest that ETF could play a role in the overexpression of the cellular oncogene EGFR.
Kageyama
R
R
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Merlino
G T
GT
Pastan
I
I
D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis; D002846:Chromatography, Affinity; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D066246:ErbB Receptors / Q000235:genetics*; D006801:Humans; D008970:Molecular Weight; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D011489:Protein Denaturation; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D014157:Transcription Factors; D004247:DNA; D066246:ErbB Receptors; D003850:Deoxyribonuclease I