Effects of pentobarbital anesthesia on the energy metabolism of murine tumors studied by in vivo 31P nuclear magnetic resonance spectroscopy.
The effects of pentobarbital anesthesia on the energy metabolism of FSaII and MCaIV foot tumors in mice were studied by 31P MRS. Using an 8.5 T spectrometer, in vivo spectra were obtained in 15 animals before and after pentobarbital anesthesia (0.05 mg/g ip). The average phosphocreatine/inorganic phosphate ratios (PCr/Pi) with and without pentobarbital were similar for both tumor histologies. Effects on individual tumors, however, were greater than 20% in 9/15 animals and greater than 50% in 6/15 animals. Pentobarbital anesthesia increased the variability of tumor intracellular pH, and the phosphomonoester/nucleotide triphosphate (PME/NTP) and nucleotide triphosphate/inorganic phosphate ratios (NTP/Pi). When examining the average in a cohort, pentobarbital anesthesia had no significant effect on the PCr/Pi, PME/NTP, NTP/Pi ratios or the pH. However, approximately equal to 50% of individual tumors do have significant changes in these parameters. The anesthesia-induced variability of tumor energy metabolism may explain the decrease in TCD50 observed in previous studies using multifraction radiation.
Okunieff
P
P
Department of Radiation Medicine, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
Rummeny
E
E
Vaupel
P
P
Skates
S
S
Willett
C
C
Neuringer
L J
LJ
Suit
H D
HD
D000758:Anesthesia / Q000379:methods; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D004307:Dose-Response Relationship, Radiation; D004734:Energy Metabolism / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D009711:Nucleotides / Q000032:analysis; D010424:Pentobarbital / Q000494:pharmacology*; D010710:Phosphates / Q000032:analysis; D010725:Phosphocreatine / Q000032:analysis; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology
D009711:Nucleotides; D010710:Phosphates; D011838:Radiation-Sensitizing Agents; D010725:Phosphocreatine; D010424:Pentobarbital