A high melting structure in DNA distinguishes phases of the cell cycle.
Differential scanning microcalorimetry of the nuclei of dividing CHO cells revealed DNA structures that showed structural transitions at 60, 76, 88, and 105 degrees C (transitions I to IV, respectively). In cultures synchronized by isoleucine deprivation the enthalpies of transitions I and II were rather constant throughout the cell cycle. While the sum of the enthalpies of III and IV was nearly constant, the ratio of IV to III varied substantially from one phase of the cycle to another. A high IV:III ratio of 6 characterized G1 while S phase gave a IV:III ratio of about 2. Cells containing metaphase chromosomes also showed a IV:III ratio near 2. The IV:III ratio for CHO cells showed a progressive decrease as the cells were maintained in isoleucine-free medium from 0 to 6 days.
Rice
G A
GA
Department of Biochemistry, University of California, Berkeley 94720.
Touchette
N A
NA
Creager
A N
AN
Goldberg
J
J
Cole
R D
RD
D000818:Animals; D002152:Calorimetry, Differential Scanning; D002453:Cell Cycle; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006358:Hot Temperature; D007399:Interphase; D007532:Isoleucine / Q000502:physiology; D007700:Kinetics; D008938:Mitosis; D010053:Ovary; D013816:Thermodynamics
D007532:Isoleucine; D004247:DNA