title: Quantitative HPLC analysis of human plaque proteins in coronary and thoracic aorta arteries.
abstract: Quantitative HPLC analysis of saline-soluble proteins obtained from human coronary and thoracic aorta plaque and from whole internal mammary artery were performed. Protein extracts were characterized by anion exchange and reverse-phase HPLC and the integrated chromatographs revealed significant differences in both peak retention times and areas for protein species from coronary artery compared to thoracic aorta artery plaque. Coronary artery plaque proteins possessed a high degree of cationic charge and polarity compared to those present in thoracic aorta plaque and normal mammary artery. This suggests that specific protein markers may be expressed in plaque of different anatomical origin, and that the processing of protein may be distinct to plaque sites. In contrast, characterization of molecular weight by gel electrophoresis resolved no major differences between plaque types. These findings indicate that proteins in human plaque lesions of different anatomical origin can be resolved by HPLC methodology and that they exhibit different charge and polarity. Such an HPLC approach may prove useful in the quantitative identification and ultimate isolation of specific protein markers present in plaque during atherogenesis, and in the study of mechanisms of protein involvement in plaque formation.
authors.affiliation: Division of Biology and Medicine, University of California, Berkeley 94720.
authors.forename: R P
authors.initials: RP
authors.lastname: Liburdy
authors.forename: N L
authors.initials: NL
authors.lastname: Burgess
authors.forename: J C
authors.initials: JC
authors.lastname: Bartley
authors.forename: B P
authors.initials: BP
authors.lastname: Mindich
mesh_terms: D001013:Aorta, Thoracic / Q000032:analysis*; D001161:Arteriosclerosis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003331:Coronary Vessels / Q000032:analysis*; D006801:Humans; D008323:Mammary Arteries / Q000032:analysis