title: Application of an enzyme-linked immunosorbent assay for screening of T-2 toxin-producing Fusarium spp.
abstract: Culture filtrates of Fusarium species were subjected without clean-up procedures to an indirect competitive enzyme-linked immunosorbent assay with anti-T-2 toxin monoclonal antibody. Fusarium sporotrichioides, F. poae, F. tricinctum, and F. culmorum strains were positive for T-2 toxin, with a minimum detection limit of 5 pg per assay (100 pg/ml of culture filtrate), and the assay data correlated well with the gas-liquid chromatographic data.
authors.affiliation: Department of Toxicology and Microbial Chemistry, Science University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nagayama
authors.forename: O
authors.initials: O
authors.lastname: Kawamura
authors.forename: K
authors.initials: K
authors.lastname: Ohtani
authors.forename: J C
authors.initials: JC
authors.lastname: Ryu
authors.forename: D
authors.initials: D
authors.lastname: Latus
authors.forename: L
authors.initials: L
authors.lastname: Sudheim
authors.forename: Y
authors.initials: Y
authors.lastname: Ueno
mesh_terms: D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D005670:Fusarium / Q000378:metabolism*; D009183:Mycotoxins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D009183:Mycotoxins