Effect of diosmin upon red blood cell deformability and osmotic fragility. Relationship with lipid content.
Rats were treated by ingesting forcibly 2 ml of a suspension of four different doses (100, 200, 300 and 400 mg/kg) of diosmin in carboxymethylcellulose (CMC) and were killed immediately or after 3 or 6 hours of fasting. Animals treated by CMC only in a similar way (gavage) exhibited a fall in red blood cell (RBC) membrane cholesterol and an increase in RBC rigidity at the third hour while osmotic fragility remained stable. Diosmin treatment opposed the rise in RBC rigidity evoked by the gavage and induced a dose-dependent decrease of the RBC membrane cholesterol over phospholipid ratio.
Garnier
M
M
University of North Paris, School of Medicine, Laboratory of Experimental and Clinical Pharmacology, Bobigny, France.
Perret
G
G
Pilardeau
P
P
Vaysse
J
J
Rolland
Y
Y
Uzzan
B
B
Vassy
R
R
D000818:Animals; D002784:Cholesterol / Q000097:blood; D004145:Diosmin / Q000494:pharmacology*; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004910:Erythrocyte Membrane / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D008055:Lipids / Q000097:blood*; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects; D010713:Phosphatidylcholines / Q000097:blood; D010714:Phosphatidylethanolamines / Q000097:blood; D010743:Phospholipids / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D013109:Sphingomyelins / Q000097:blood; D014280:Triglycerides / Q000097:blood
D005419:Flavonoids; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D013109:Sphingomyelins; D014280:Triglycerides; D004145:Diosmin; D002784:Cholesterol