Selective activation of transcription by a novel CCAAT binding factor.
A novel CCAAT binding factor (CBF) composed of two different subunits has been extensively purified from rat liver. Both subunits are needed for specific binding to DNA. Addition of this purified protein to nuclear extracts of NIH 3T3 fibroblasts stimulates transcription from several promoters including the alpha 2(I) collagen, the alpha 1(I) collagen, the Rous sarcoma virus long terminal repeat (RSV-LTR), and the adenovirus major late promoter. Point mutations in the CCAAT motif that show either no binding or a decreased binding of CBF likewise abolish or reduce activation of transcription by CBF. Activation of transcription requires, therefore, the specific binding of CBF to its recognition sites.
Maity
S N
SN
Department of Molecular Genetics, Univesity of Texas, M.D. Anderson Cancer Center, Houston 77030.
Golumbek
P T
PT
Karsenty
G
G
de Crombrugghe
B
B
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D003094:Collagen / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology*; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D051379:Mice; D009687:Nuclear Proteins / Q000502:physiology; D011401:Promoter Regions, Genetic; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D014157:Transcription Factors; D003094:Collagen