Interaction of 7-acetyltaxol with different tubulin assemblies.
Equilibrium microdialysis of [3H]acetyltaxol against different tubulin assemblies showed that: (i) the binding capacity of tubulin does not depend on the temperature; (ii) two classes of 'polymers' exist, with respect to Ac-taxol binding. Some of them (plaques, complex cylinders induced with some polycations and spirals made with rhazinilam) bound Ac-taxol, as do normal microtubules. In contrast, spirals formed with vinblastine and griseofulvin, rings made with polycations and complex cylinders induced with spermine do not bind Ac-taxol as is the case with free tubulin.
Takoudju
M
M
Laboratoire de Pharmacologie et de Toxicologie Fondamentales, Centre National de la Recherche Scientifique, Toulouse, France.
Wright
M
M
Chenu
J
J
Guéritte-Voegelein
F
F
Guénard
D
D
D000470:Alkaloids / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry; D003956:Dialysis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004126:Dimethylformamide / Q000494:pharmacology; D006118:Griseofulvin / Q000494:pharmacology; D007212:Indolizines; D007769:Lactams; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D017239:Paclitaxel / Q000031:analogs & derivatives*; D011107:Polylysine / Q000494:pharmacology; D011479:Protamines / Q000494:pharmacology; D012756:Sheep; D013096:Spermine / Q000494:pharmacology; D013431:Sulfates / Q000494:pharmacology; D043823:Taxoids; D013696:Temperature; D014404:Tubulin / Q000378:metabolism*; D014747:Vinblastine / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology; D019287:Zinc Sulfate
D000470:Alkaloids; D007212:Indolizines; D007769:Lactams; D046911:Macromolecular Substances; D011479:Protamines; D013431:Sulfates; D043823:Taxoids; D014404:Tubulin; D011107:Polylysine; D013096:Spermine; D006118:Griseofulvin; C009170:rhazinilam; D014747:Vinblastine; D019287:Zinc Sulfate; D004126:Dimethylformamide; C043189:7-acetyltaxol; D015032:Zinc; D017239:Paclitaxel; D004121:Dimethyl Sulfoxide