Purification and characterization of human placental ferredoxin.
A ferredoxin-type iron-sulfur protein was isolated from human placenta mitochondria. The properties of the purified protein were very similar to those of adrenal ferredoxin (adrenodoxin), and immunological cross-reactivity with polyclonal antibodies to bovine adrenodoxin was observed. The N-terminal amino acid sequence and the visible absorption spectrum were identical to bovine adrenodoxin. The molecular mass as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Mr approximately 13,500), however, is slightly smaller than that of adrenodoxin, and the C-terminal sequence is different. Human placental ferredoxin can substitute for bovine adrenodoxin in reactions reconstituted with bovine adrenal enzymes which catalyze the side chain cleavage of cholesterol to pregnenolone and the 11 beta-hydroxylation of deoxycorticosterone to corticosterone.
Coghlan
V M
VM
Department of Biological Chemistry, University of California, Irvine 92717.
Cupp
J R
JR
Vickery
L E
LE
D000311:Adrenal Glands / Q000032:analysis; D000325:Adrenodoxin / Q000378:metabolism; D000595:Amino Acid Sequence; D002784:Cholesterol / Q000378:metabolism; D002845:Chromatography; D003900:Desoxycorticosterone / Q000378:metabolism; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005288:Ferredoxins / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008928:Mitochondria / Q000032:analysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D013053:Spectrophotometry
D005288:Ferredoxins; D000325:Adrenodoxin; D003900:Desoxycorticosterone; D002784:Cholesterol