title: Characterization of the genes for fructose-bisphosphate aldolase in Trypanosoma brucei.
abstract: In Trypanosoma brucei stock 427 the glycolytic enzyme fructose-bisphosphate aldolase is encoded by two tandemly linked genes of identical sequence. Such a tandem arrangement of aldolase genes is also present in other T. brucei stocks of unrelated origin. In stock 427 one of the allelic genes is a pseudogene, as a result of a one-nucleotide deletion. The genes code for a polypeptide of 371 amino acids, with a calculated molecular weight of 40,940. The protein that is predicted from the gene sequence has 45-48% positional identity with known aldolase sequences of other organisms. The trypanosomal protein is, however, unique in having a 10 amino-acid insertion near its N-terminus and high number of basic residues, a feature it shares with other glycolytic enzymes of T. brucei. These glycolytic enzymes have in common that they are located in microbody-like organelles, the glycosomes. We have previously proposed that the positively charged residues may be involved in the import of the proteins into the organelles.
authors.affiliation: International Institute of Cellular and Molecular Pathology, Research Unit for Tropical Diseases, Brussels, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Marchand
authors.forename: A
authors.initials: A
authors.lastname: Poliszczak
authors.forename: W C
authors.initials: WC
authors.lastname: Gibson
authors.forename: R K
authors.initials: RK
authors.lastname: Wierenga
authors.forename: F R
authors.initials: FR
authors.lastname: Opperdoes
authors.forename: P A
authors.initials: PA
authors.lastname: Michels
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics*
chemical_list: D004247:DNA; D005634:Fructose-Bisphosphate Aldolase