A simple planimetric method to quantify cytotoxicity in cell culture monolayers.
Planimetry was shown to be a rapid, simple and reproducible method to quantify gross cytotoxicity in cell culture monolayers. A shellfish extract prepared from blue mussels (Mytilus edulis) was cytotoxic to Buffalo green monkey kidney cells. Exposure of cells to mussel extracts for 1, 2 and greater than or equal to 4 h followed by agar (plaque assay) overlays produced 34, 87, and 100% destruction of the monolayers, respectively, within 72 h. Planigraphs, prepared from tracing areas of cytotoxicity onto sheets of paper, served as permanent records of toxicity which were easily measured by planimeter experienced and nonexperienced scientists. Planimetric procedures may have utility in measuring biological and chemical toxicity as well as physiological stress in cell monolayers.
Richards
G P
GP
National Marine Fisheries Service, Southeast Fisheries Center, Charleston Laboratory, South Carolina 29412.
Bemiss
J A
JA
Sample
J D
JD
D000818:Animals; D049872:Bivalvia; D002470:Cell Survival; D002478:Cells, Cultured; D014020:Tissue Extracts / Q000633:toxicity*
D014020:Tissue Extracts