Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes.
Bovine follicular oocytes surrounded by cumulus cells for more than one-third of their surface were matured, fertilized and developed in vitro utilizing a co-culture system with bovine cumulus cells. Embryos developed into blastocysts were non-surgically transferred to the uteri of cows at Day 6, 7 or 8 (Day 0 = oestrus). Out of 6 recipient cows (19 blastocysts transferred), 3 became pregnant. One of the 3 pregnant cows carried twins. The results of this study demonstrated the viability of embryos obtained from in-vitro maturation of bovine oocytes followed by in-vitro fertilization and culture to the blastocyst stage in vitro.
Goto
K
K
Department of Animal Science, Faculty of Agriculture, Kagoshima University, Japan.
Kajihara
Y
Y
Kosaka
S
S
Koba
M
M
Nakanishi
Y
Y
Ogawa
K
K
D000818:Animals; D002417:Cattle; D004624:Embryo Transfer / Q000379:methods* / Q000662:veterinary; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D009865:Oocytes; D006080:Ovarian Follicle / Q000166:cytology; D011247:Pregnancy