title: Lymphocyte phenotypes in the intestinal mucosa of sheep infected with Trichostrongylus colubriformis.
abstract: Monoclonal antibodies to ovine lymphocyte surface antigens were used in an immunohistochemical study of the intestine of sheep. In the epithelium CD8+ cells predominated whereas the majority of lamina propria T lymphocytes were CD4+. Infection of sheep with the parasitic nematode Trichostrongylus colubriformis including sufficiently large numbers of parasites to induce protective immunity did not alter the number of CD4+ or CD8+ lymphocytes in the intestinal mucosa. In contrast, exposure of naive sheep to a single large infection of T. colubriformis resulted in a substantial decrease in number of CD8+ cells and moderate decreases in number of CD4+ cells in the duodenal but not the jejunal mucosa. MHC class II antigens were not detected either in or on epithelial cells of the sheep small intestine.
authors.affiliation: Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: M D
authors.initials: MD
authors.lastname: Gorrell
authors.forename: G
authors.initials: G
authors.lastname: Willis
authors.forename: M R
authors.initials: MR
authors.lastname: Brandon
authors.forename: A K
authors.initials: AK
authors.lastname: Lascelles
mesh_terms: D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D004386:Duodenum / Q000276:immunology; D005333:Fetus; D007413:Intestinal Mucosa / Q000276:immunology*; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D012756:Sheep; D014252:Trichostrongyloidiasis / Q000276:immunology*; D014253:Trichostrongylosis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface