Application of an enzyme-linked immunosorbent assay for screening of T-2 toxin-producing Fusarium spp.
Culture filtrates of Fusarium species were subjected without clean-up procedures to an indirect competitive enzyme-linked immunosorbent assay with anti-T-2 toxin monoclonal antibody. Fusarium sporotrichioides, F. poae, F. tricinctum, and F. culmorum strains were positive for T-2 toxin, with a minimum detection limit of 5 pg per assay (100 pg/ml of culture filtrate), and the assay data correlated well with the gas-liquid chromatographic data.
Nagayama
S
S
Department of Toxicology and Microbial Chemistry, Science University of Tokyo, Japan.
Kawamura
O
O
Ohtani
K
K
Ryu
J C
JC
Latus
D
D
Sudheim
L
L
Ueno
Y
Y
D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D005670:Fusarium / Q000378:metabolism*; D009183:Mycotoxins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology
D000911:Antibodies, Monoclonal; D009183:Mycotoxins