title: A ligase-mediated gene detection technique.
abstract: An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena 91125.
authors.forename: U
authors.initials: U
authors.lastname: Landegren
authors.forename: R
authors.initials: R
authors.lastname: Kaiser
authors.forename: J
authors.initials: J
authors.lastname: Sanders
authors.forename: L
authors.initials: L
authors.lastname: Hood
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D011088:DNA Ligases / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism; D005456:Fluorescent Dyes; D005914:Globins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D011118:Polynucleotide Ligases / Q000378:metabolism*
chemical_list: D004274:DNA, Recombinant; D005456:Fluorescent Dyes; D005914:Globins; D004247:DNA; D011088:DNA Ligases; D011118:Polynucleotide Ligases