Pertussis toxin-catalysed ADP-ribosylation of endometrial proteins in sheep.
Membrane fractions prepared from the uterine endometrium of untreated ovariectomized sheep contained a 41 x 10(3) Mr protein that was [32P]ADP-ribosylated by pertussis toxin in the presence of [32P]NAD. Progestin and progestin plus oestrogen treatment in vivo increased the concentration of this protein 2.7- and 3.6-fold respectively. Endometrial extracts from untreated or progestin-treated sheep also contained proteins of Mr 69 x 10(3) and 120 x 10(3) which were ADP-ribosylated in the absence of pertussis toxin; these proteins were not ADP-ribosylated in sheep receiving oestrogen. Incubation of endometrial slices from progestin plus oestrogen-treated sheep with oxytocin in vitro increased phosphoinositide hydrolysis 11-fold. This effect was not altered by prior incubation with pertussis toxin, although toxin treatment reduced by 64% subsequent labelling of the 41 x 10(3) Mr protein when membrane fractions prepared from pretreated slices were incubated with pertussis toxin and [32P]NAD. Thus the endometrium contains a pertussis toxin-sensitive protein which is induced by steroid treatment, but this protein is not involved in the phosphoinositide response to oxytocin.
Flint
A P
AP
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D037342:Pertussis Toxin; D011372:Progestins / Q000494:pharmacology; D012756:Sheep; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D004967:Estrogens; D008565:Membrane Proteins; D011372:Progestins; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D037342:Pertussis Toxin