Systemic acetyl-L-carnitine elevates nigral levels of glutathione and GABA.
Amino acid and reduced glutathione (GSH) levels in substantia nigra (SN) as well as striatal monoamine levels were measured in acetyl-L-carnitine (ALCar) treated and control Swiss-Webster mice. ALCar, L carnitine, or saline were administered i.p. to mice for 5 days and mice were decapitated 24 hours following the last injection. Substantia nigra and striata were isolated within 2.5 and 3 min., respectively, and frozen immediately on dry ice. A significant dose-dependent increase of nigral GABA was observed following ALCar treatment; GABA levels were also increased by administration of carnitine. Nigral GSH levels were also increased. Striatal levels of dopamine and metabolites were not significantly affected by ALCar or carnitine. These results, suggest that ALCar may be useful in treating symptoms of neuronal dysfunction related to accumulation of metabolic waste.
Fariello
R G
RG
Department of Neurological Sciences, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL.
Ferraro
T N
TN
Golden
G T
GT
DeMattei
M
M
D000108:Acetylcarnitine / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives*; D002531:Cerebellum / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism*; D008297:Male; D051379:Mice; D013378:Substantia Nigra / Q000187:drug effects* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000596:Amino Acids; D005680:gamma-Aminobutyric Acid; D000108:Acetylcarnitine; D005978:Glutathione; D002331:Carnitine