Nuclear matrix-intermediate filament system and its alteration in adenovirus infected HeLa cell.
The nuclear matrix-intermediate filament (NM-IF) of normal and adenovirus infected HeLa cells were investigated by means of both electron microscopy and two-dimensional gel electrophoresis. After infection there were some changes in NM-IF, and the viral factory was suspended in nuclear matrix. Two new polypeptides appeared in 2-D gel of NM-IF after infection. They are probably not viral proteins but cellular polypeptides. During viral replication, all of vimentin were degraded and phosphorylated keratin 18 increased. These facts suggest that NM-IF plays a certain role in adenovirus replication.
Zhai
Z H
ZH
Department of Biology, Peking University Beijing, People's Republic of China.
Wang
X
X
Qian
X Y
XY
D000260:Adenoviruses, Human / Q000502:physiology*; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D007382:Intermediate Filaments / Q000032:analysis / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000032:analysis; D014746:Vimentin / Q000032:analysis; D014779:Virus Replication
D009687:Nuclear Proteins; D014746:Vimentin