Plasma haemoglobin determination using chlorpromazine as a non-carcinogenic reagent.
Plasma haemoglobin was assayed with the non-carcinogenic reagent phenothiazine. This method is sensitive and allows the measurement of plasma haemoglobin concentrations in the range 4-500 mg/l with a within-run CV of 2.1%, and a between-run CV of 4.3%. A spectrophotometric scanning method (x) based on the determination of haemoglobin as haemiglobin cyanide using the Soret band at 419 nm correlated well with the phenothiazine method (y): y = 1.07x + 15.8, r = 0.995, n = 31. It was found that the absorbances in the phenothiazine method were markedly dependent on the concentration of phosphoric acid.
Nyyssönen
K
K
Department of Clinical Chemistry, Kuopio University Central Hospital, Finland.
Parviainen
M T
MT
Penttilä
I M
IM
D002746:Chlorpromazine; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D013053:Spectrophotometry
D006454:Hemoglobins; D007202:Indicators and Reagents; D002746:Chlorpromazine