Measurement of trazodone using solid-phase extraction and wide-bore capillary gas chromatography with nitrogen-selective detection.
A method for the determination of the antidepressant drug trazodone is presented. 8-Hydroxyloxapine is used as the internal standard. A simple solid-phase extraction procedure utilizing disposable reversed-phase C18 columns is described. Samples are analyzed by gas chromatography with nitrogen-selective detection using a wide-bore capillary column with a permanently bonded, nonpolar stationary phase. The assay possesses linearity to 3.0 micrograms/mL, sensitivity to at least 0.25 microgram/mL, recovery averaging 96%, and between-run precision reflected by a CV of 5.6%. We conclude that the method reported here is ideally suited for monitoring therapeutic and toxic levels of trazodone.
Rifai
N
N
Department of Pathology, University of North Carolina School of Medicine, Chapel Hill 27514.
Levtzow
C B
CB
Howlett
C M
CM
Phillips
J C
JC
Parker
N C
NC
Cross
R E
RE
D002849:Chromatography, Gas / Q000295:instrumentation; D014196:Trazodone / Q000032:analysis*
D014196:Trazodone