A rapid method for processing very large numbers of tissue sections for immunohistochemical hybridoma screening.
A hybridoma screening format which facilitates the processing of thousands of tissue sections for immunohistochemical analysis is described. The method utilizes sterile replica transfers of monoclonal antibody-containing test supernatants from 96-well culture plates to tissue sections mounted in appropriately sized 8 X 12 arrays, and is extremely rapid and inexpensive.
Marusich
M F
MF
Department of Biology, University of Oregon, Eugene 97403.
D000818:Animals; D002459:Cell Fusion; D002474:Cell-Free System; D003470:Culture Media; D005629:Frozen Sections / Q000295:instrumentation / Q000379:methods*; D006825:Hybridomas / Q000032:analysis*; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008867:Microtomy / Q000379:methods*
D003470:Culture Media