Lipoprotein secretion by human monocyte derived macrophages.
Cholesterol-loaded human monocyte derived macrophages secrete distinct class of lipoprotein. Following macrophages incubation in serum-free medium containing [14C]-oleic acid the cells secrete lipoprotein associated radioactivity that was found in triglycerides, phospholipids and cholesteryl ester. Macrophage lipoprotein secretion was analyzed by non-denatured gradient gel electrophoresis, agarose lipoprotein electrophoresis and discontinuous density gradient ultracentrifugation. The lipoprotein secreted by human macrophages was shown to be triglyceride-enriched and contain a protein resembling apolipoprotein E.
Aviram
M
M
Department of Medicine, University of Washington, Seattle.
D001057:Apolipoproteins E / Q000097:blood; D002784:Cholesterol / Q000097:blood; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D010743:Phospholipids / Q000097:blood; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation
D001057:Apolipoproteins E; D008074:Lipoproteins; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol