Establishment of the nuclear location of Dictyostelium discoideum plasmids.
The nuclear location of the Dictyostelium discoideum plasmids was studied using a biochemical approach based on the presence of plasmid sequences in nucleosomes. This analysis revealed that all four of the known plasmids (Ddp1, Ddp2, Ddp3, Ddp5) are present in chromatin. This evidence establishes that the D. discoideum plasmids are not cytoplasmic but located in the nucleus. D. discoideum is unique among eukaryotes in possessing a group of nonhomologous endogenous plasmids in its nucleus. These plasmids are excellent starting material for construction of nuclear transformation and expression vectors. Such vectors upon transformation into D. discoideum are also present in chromatin as expected for DNA located in the nucleus.
Ashktorab
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Department of Biology, Utah State University, Logan 84322-5500.
Welker
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D002467:Cell Nucleus / Q000502:physiology*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004023:Dictyostelium / Q000235:genetics*; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D009707:Nucleosomes / Q000502:physiology; D010957:Plasmids
D004271:DNA, Fungal; D009707:Nucleosomes