The mink proopiomelanocortin gene: characterization of cDNA and chromosomal localization.
A cDNA library from the mink pituitary was screened using as probe a synthetic oligodeoxyribonucleotide, 5'-TTCATGACCTCCGA-3', corresponding to the endorphin region of bovine proopiomelanocortin (POMC) cDNA. As a result, several clones containing inserts complementary to POMC mRNA were identified. The sequence of one of the fragments (585 bp, 65% of the total length of mRNA) was determined. A high degree of homology (over 80%) among the primary structures of sequences from mink, man, and bovine cDNA POMC was established. With the cloned mink cDNA fragment as probe, the DNAs from mink-Chinese hamster hybrid clones were studied. The results of segregation analysis of mink POMC sequences and mink chromosomes in the mink-Chinese hamster panel allowed us to assign the POMC gene to mink chromosome 11.
Khlebodarova
T M
TM
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk.
Karasik
G I
GI
Matveeva
N M
NM
Serov
O L
OL
Golovin
S Y
SY
Bondar
A A
AA
Karginov
V A
VA
Morozov
I S
IS
Zelenin
S M
SM
Mertvetsov
N P
NP
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D008907:Mink / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D011333:Pro-Opiomelanocortin; D004247:DNA