Kinetic studies on nitrite and nitrate in rats by ion-pair chromatography.
A highly sensitive and specific ion-pair chromatographic method is presented for the simultaneous determination of nitrite (NO2-) and nitrate (NO3-) in plasma. The detection limits are 2 mumol NO2-/l and 8 mumol NO3-/l with recovery rates of 98-99%. The applicability of this method to clinical medicine is demonstrated using NO2- -loaded rats as a biological model: following intravenous infusion or intragastric application of NO2-, blood levels of NO2-, NO3- and methemoglobin are measured during 2-64 min. The kinetic data obtained reflect the complex metabolism of NO2- and NO3- in living organisms.
Stein
C
C
Department of Pharmacology and Toxicology of Nutrition, University of Hohenheim, FRG.
Classen
H G
HG
Schwedt
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D007700:Kinetics; D008954:Models, Biological; D009566:Nitrates / Q000097:blood*; D009573:Nitrites / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
D009566:Nitrates; D009573:Nitrites