Molecular cloning and sequence analysis of the mumps virus gene encoding the P protein: mumps virus P gene is monocistronic.
The nucleotide sequence of the P (phosphoprotein) gene of two strains of mumps virus has been determined from overlapping cDNA clones. The P gene contained a single open reading frame coding for a protein of 391 amino acids with a calculated Mr of 41,587, in good agreement with the value (40K to 45K) estimated from electrophoretic mobility on SDS-polyacrylamide gels. No open reading frame analogous to the C gene of other paramyxoviruses existed in the mumps virus P gene region. Comparison of the amino acid sequence of the mumps virus P protein with that of Newcastle disease virus showed a limited sequence homology.
Takeuchi
K
K
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Hishiyama
M
M
Yamada
A
A
Sugiura
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D010750:Phosphoproteins; D012367:RNA, Viral; D014764:Viral Proteins