title: The mechanism of action of quinocarmycin citrate (KW 2152) on mouse L1210 cells in vitro.
abstract: The effects of the antitumor antibiotic, quinocarmycin citrate (KW 2152), on L1210 cells were studied in vitro. The cellular growth was completely inhibited at 10(-6) M KW 2152, and after 2 days no viable cell was seen. The incorporation of 3H-thymidine, 3H-uridine, or 3H-leucine into the acid-insoluble fraction was not affected at 10(-4) M for 1 h; however, when the cells were treated with 10(-6) M for 24 h, the radioactivity appearing in the acid-insoluble fraction was reduced to 20%, 30%, and 48%, respectively, of the control. The single strand scission of the DNA of L1210 cells was seen at 10(-7) M for 24 h, as revealed by an alkaline, sucrose density gradient. However, no damage to plasmid pBR322 was observed even at 10(-6) M KW 2152 for 24 h, as revealed by 0.8% agarose gel electrophoresis, indicating that some soluble factors of the cells might contribute to the damage to the DNA of L1210 cells. The processing of pre-rRNA of the cells was not inhibited at 10(-6) M of the drug for 24 h of incubation.
authors.affiliation: Department of Clinical Cancer Chemotherapy, Tohoku University, Sendai, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kanamaru
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
authors.forename: C
authors.initials: C
authors.lastname: Ishioka
authors.forename: H
authors.initials: H
authors.lastname: Kakuta
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: A
authors.initials: A
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Asamura
authors.forename: A
authors.initials: A
authors.lastname: Wakui
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D004273:DNA, Neoplasm / Q000187:drug effects; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010957:Plasmids / Q000187:drug effects; D012334:RNA, Neoplasm / Q000187:drug effects; D012335:RNA, Ribosomal / Q000187:drug effects
chemical_list: D000903:Antibiotics, Antineoplastic; D004273:DNA, Neoplasm; D007546:Isoquinolines; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; C038710:quinocarcin