Protein synthesis in human platelets.
The platelets and leukocytes of human peripheral blood were separated and cultured for 24 h in the same medium. The culture medium was completed with S-methionine. The overall protein synthetic activity of the platelets, compared with leukocytes, was studied with a two-dimensional gel electrophoresis technique combined with fluorographic detection of labelled proteins. Our findings confirm the platelet-origin of a few newly synthesized proteins. The most pronounced newly synthesized platelet protein with a molecular weight of approximately 35 Kda and with a more acidic isoelectric point than that of actin was identified as a cytoskeleton-associated protein.
Ludány
A
A
Department of Clinical Chemistry, Medical University Pécs, Hungary.
Kellermayer
M
M
D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D007962:Leukocytes / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D013462:Sulfur Radioisotopes
D001798:Blood Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine