[Activity and properties of superoxide dismutase from human erythrocytes and plasma in ontogenesis].
One- and two-substituted potassium-salts of phosphoric acid are studied for their effect on the value of superoxide dismutase (SOD) activity in the plasma and erythrocytes of healthy adults and newborn children. It is shown that the superoxide dismutase activity of plasma depends on the phosphoric acid salts which are used during its treatment. Single direction of changes in the activity of superoxide dismutase obtained after the plasma treatment by KH2PO4 and K2HPO4 depending on the pH incubation medium, the results of separation of protein fractions obtained by the method of disc-electrophoresis testify that the superoxide dismutase activity is connected with the same protein. Different absolute values of the plasma SOD activity and different influence on it of azide sodium are, probably, determined by spatial orientation of copper ions in the active centre, which changes depending on pH of the medium. Superoxide dismutase plasma and erythrocytes in newborn children have higher activity than is adults.
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D000293:Adolescent; D000328:Adult; D000375:Aging / Q000378:metabolism*; D001386:Azides / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D010084:Oxidation-Reduction; D010756:Phosphoric Acids / Q000494:pharmacology; D019810:Sodium Azide; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000097:blood*
D001386:Azides; D010756:Phosphoric Acids; D019810:Sodium Azide; D013482:Superoxide Dismutase
Aktivnost' i svoĭstva superoksiddismutazy éritrotsitov i plazmy cheloveka v ontogeneze.