title: Replication termination for staphylococcal plasmids: plasmids pT181 and pC221 cross-react in the termination process.
abstract: We present data which indicate that (i) the origin of replication of plasmids pT181 and pC221 can also function as termination signals; (ii) termination of replication occurs when a round of replication initiated either by RepC at the pT181 origin or by RepD at the pC221 origin reaches either of these origins, proving that the two plasmids cross-react for termination of replication; and (iii) the replication initiated at the origin of another staphylococcal plasmid, pE194, does not terminate at the origin of pT181 or pC221, indicating the existence of a specific relationship between the initiation and termination of a replication event.
authors.affiliation: Department of Plasmid Biology, Public Health Research Institute, New York, New York 10016.
authors.forename: S
authors.initials: S
authors.lastname: Iordanescu
authors.forename: S J
authors.initials: SJ
authors.lastname: Projan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003429:Cross Reactions; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004587:Electrophoresis, Agar Gel; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D013211:Staphylococcus aureus / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C043650:RepC protein, Staphylococcus aureus