title: Detection of biotinylated nucleic acid hybrids by antibody-coated gold colloid.
abstract: A simple and sensitive nonradioactive method for detecting biotinylated nucleic acid hybrids on nitrocellulose paper is described. The method employs anti-biotin antibody-coated gold colloid and silver enhancement. Comparative results indicate sensitivity similar to that of a commonly used enzymatic colorimetric detection method.
authors.affiliation: Molecular Diagnostics Inc., Miles Research Center, West Haven, Connecticut 06516.
authors.forename: S
authors.initials: S
authors.lastname: Tomlinson
authors.forename: A
authors.initials: A
authors.lastname: Lyga
authors.forename: E
authors.initials: E
authors.lastname: Huguenel
authors.forename: N
authors.initials: N
authors.lastname: Dattagupta
mesh_terms: D000345:Affinity Labels; D000906:Antibodies; D001710:Biotin / Q000276:immunology; D003101:Collodion; D003102:Colloids; D004247:DNA / Q000032:analysis; D006046:Gold; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D010316:Particle Size
chemical_list: D000345:Affinity Labels; D000906:Antibodies; D003102:Colloids; D009696:Nucleic Acids; D001710:Biotin; D006046:Gold; D003101:Collodion; D004247:DNA