[Highly purified, functionally active human fibronectin preparation].
Fibronectin has been purified by gelatin-Sepharose affinity chromatography from fresh frozen human plasma. The bound fibronectin was eluted with 3 M urea. The purity of the fibronectin obtained has been checked on (immunoelectrophoresis, polyacrylamide gel electrophoresis, FPLC). Biological activity of the purified molecule has been monitored by means of three assays: quantitation of the gelatin-binding activity by ELISA, quantitation of the fibronectin-mediated attachment of fibroblasts on plastic and evaluation of the opsonic activity (uptake of gelatin latex particles by a murine macrophage line). When deep-frozen, fibronectin retains all of its properties. This highly purified and functional fibronectin fulfills the basic requirements for a standard reagent. It will allow to investigate physicochemical and functional alterations of various fibronectins.
Regnault
V
V
Unité INSERM U. 284, Vandoeuvre-Les-Nancy.
Rivat
C
C
Maugras
M
M
Stoltz
J F
JF
D002448:Cell Adhesion / Q000187:drug effects; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000302:isolation & purification* / Q000494:pharmacology / Q000592:standards; D005780:Gelatin / Q000378:metabolism; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007841:Latex Fixation Tests; D009895:Opsonin Proteins / Q000378:metabolism
D005353:Fibronectins; D009895:Opsonin Proteins; D005780:Gelatin
Préparation de fibronectine humaine de haute pureté fonctionnellement active.