title: Further studies on the resolution of human mercapt- and nonmercaptalbumin and on human serum albumin in the elderly by high-performance liquid chromatography.
abstract: High-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on Asahipak GS-520 showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the secondary one to nonmercaptalbumin (HNA). HPLC analysis of HSA on Asahipak ES-520 N showed three peaks, the principal component corresponding to HMA, the secondary one to HNA having mixed disulfide with cysteine or glutathione and the tertiary one to HNA oxidized higher than mixed disulfide. Two kinds of rapid HPLC for the resolution of HSA into HMA and HNA were developed by the present authors. Using these HPLC, the present authors found a significant decrease in the fraction of HMA in the elderly.
authors.affiliation: Department of Physiology, School of Medicine, Gifu University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Era
authors.forename: T
authors.initials: T
authors.lastname: Hamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Sogami
authors.forename: K
authors.initials: K
authors.lastname: Kuwata
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Kawai
authors.forename: Y
authors.initials: Y
authors.lastname: Kitazawa
authors.forename: H
authors.initials: H
authors.lastname: Okabe
authors.forename: A
authors.initials: A
authors.lastname: Noma
mesh_terms: D000368:Aged; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000032:analysis; D006801:Humans; D012016:Reference Values; D012709:Serum Albumin / Q000302:isolation & purification*
chemical_list: D004220:Disulfides; D012709:Serum Albumin; C001702:mercaptoalbumin