title: Immobilized wheat germ agglutinin separates small oligosaccharides derived from poly-N-acetyllactosaminoglycans of embryonal carcinoma cells.
abstract: Five pure oligosaccharides derived from poly-N-acetyllactosaminoglycans of teratocarcinoma cells were chromatographed on immobilized wheat germ agglutinin (WGA). Three of them, Gal beta 1-4GlcNAc, GlcNAc beta 1-3Gal, and GlcNAc beta 1-3Gal beta 1-4GlcNAc, revealed only weak binding, but GlcNAc beta 1-6Gal beta 1-4GlcNAc was bound moderately and GlcNAc beta 1-6Gal was bound quite strongly. The differences in the binding affinities were large enough to allow chromatographic separation of the five oligosaccharides into three distinct fractions in a small column of WGA-agarose.
authors.affiliation: Department of Biochemistry, University of Helsinki, Finland.
authors.forename: O
authors.initials: O
authors.lastname: Renkonen
authors.forename: P
authors.initials: P
authors.lastname: Mäkinen
authors.forename: K
authors.initials: K
authors.lastname: Hård
authors.forename: J
authors.initials: J
authors.lastname: Helin
authors.forename: L
authors.initials: L
authors.lastname: Penttilä
mesh_terms: D000606:Amino Sugars / Q000032:analysis*; D000818:Animals; D002847:Chromatography, Agarose; D051379:Mice; D009844:Oligosaccharides / Q000302:isolation & purification*; D011134:Polysaccharides / Q000032:analysis*; D013724:Teratoma / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000032:analysis; D014909:Wheat Germ Agglutinins
chemical_list: D000606:Amino Sugars; D009844:Oligosaccharides; D011134:Polysaccharides; D014909:Wheat Germ Agglutinins