title: Site-directed ELISA identifies a highly antigenic region of the simian immunodeficiency virus transmembrane glycoprotein.
abstract: The transmembrane glycoprotein (gp32) of the simian immunodeficiency virus (SIV) contains a highly antigenic region that includes amino acid residues 606-628. A synthetic peptide representing this region was highly immunoreactive with sera from SIV-infected primates in a site-directed enzyme-linked immunosorbent assay (ELISA). This reactivity extended across four primate species from three genera and identified infection with at least two distinct isolates of SIV. This site-directed ELISA represents a simple, accessible method with broad specificity for screening large numbers of primates for antibodies against SIV.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD.
authors.forename: P R
authors.initials: PR
authors.lastname: Johnson
authors.forename: D E
authors.initials: DE
authors.lastname: Parks
authors.forename: E
authors.initials: E
authors.lastname: Norrby
authors.forename: R A
authors.initials: RA
authors.lastname: Lerner
authors.forename: R H
authors.initials: RH
authors.lastname: Purcell
authors.forename: R M
authors.initials: RM
authors.lastname: Chanock
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000382:microbiology*; D012190:Retroviridae / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000032:analysis*
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins