Normal human peritoneal macrophages are unable to cap and internalize class II antigens.
Normal human peritoneal macrophages show a restricted capacity to differentiate into inflammatory macrophages in vivo. We now report that these cells are unable to cap and internalize HLA-DR, as compared to endometriosis, and other macrophages. Immunoelectron microscopy indicated that lack of modulation was not due to the presence of preclustered antigenic sites. Northern blot analysis demonstrated transcripts for HLA.DR, c-fms, and c-fos, indicating that the surface defects were not likely to be associated with a general depression of transcriptional activity. There was no correlation between the mobility of class II molecules and the ability to present antigen as determined by autologous lymphocyte responses to tetanus toxoid. The inability of normal peritoneal macrophages to modulate class II antigens may represent a normal and more general environmental alteration required to permit peritoneal cells a scavenging function without developing the deleterious effects leading to a peritoneal inflammatory response.
Haskill
S
S
Department of Obstetrics and Gynecology, School of Medicine, University of North Carolina, Chapel Hill 27599.
Becker
S
S
Jontell
M
M
Sporn
S
S
Halme
J
J
D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D000954:Antigens, Surface / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004715:Endometriosis / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry; D006681:HLA-D Antigens / Q000378:metabolism*; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D007152:Immunologic Capping; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D010529:Peritoneal Cavity / Q000166:cytology; D013745:Tetanus Toxoid / Q000276:immunology; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003432:Cross-Linking Reagents; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D013745:Tetanus Toxoid