title: NMR-invisible lactate in blood plasma.
abstract: Resonances for lactate are broadened in 500 MHz 1H NMR spectra of human blood plasma and only about one-third is visible in Hahn spin-echo spectra. Similar effects are observed for some other carboxylate anions. Lactate added to the high-Mr fraction of plasma can give rise to peaks which are too broad to observe in either single-pulse or spin-echo spectra. Addition of agents such as NH4Cl of SDS dramatically increases the intensities of lactate peaks. Some glycoproteins appear to broaden lactate resonances.
authors.affiliation: Department of Chemistry, Birkbeck College, University of London, England.
authors.forename: J D
authors.initials: JD
authors.lastname: Bell
authors.forename: J C
authors.initials: JC
authors.lastname: Brown
authors.forename: G
authors.initials: G
authors.lastname: Kubal
authors.forename: P J
authors.initials: PJ
authors.lastname: Sadler
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; D007773:Lactates; D000643:Ammonium Chloride; D019344:Lactic Acid; D012967:Sodium Dodecyl Sulfate