title: Cloning and expression of human nebulin cDNAs and assignment of the gene to chromosome 2q31-q32.
abstract: We have isolated two nonoverlapping cDNAs encoding human nebulin, a muscle-specific protein. Northern hybridization analysis shows that nebulin is encoded by a huge message at least 25 kb in length. By hybridizing two nonoverlapping cDNAs to DNA isolated from rodent X human cell hybrids, we assign this presumably single-copy gene to human chromosome 2; sublocalization studies indicate that the nebulin gene is on the long arm of the chromosome, in the region 2q31-q32.
authors.affiliation: H. Houston Merritt Clinical Research Center for Muscular Dystrophy and Related Disorders, Columbia University, New York, New York 10032.
authors.forename: M
authors.initials: M
authors.lastname: Zeviani
authors.forename: B T
authors.initials: BT
authors.lastname: Darras
authors.forename: R
authors.initials: R
authors.lastname: Rizzuto
authors.forename: G
authors.initials: G
authors.lastname: Salviati
authors.forename: R
authors.initials: R
authors.lastname: Betto
authors.forename: E
authors.initials: E
authors.lastname: Bonilla
authors.forename: A F
authors.initials: AF
authors.lastname: Miranda
authors.forename: J
authors.initials: J
authors.lastname: Du
authors.forename: C
authors.initials: C
authors.lastname: Samitt
authors.forename: G
authors.initials: G
authors.lastname: Dickson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D011817:Rabbits
chemical_list: D009124:Muscle Proteins; C035620:nebulin; D004247:DNA