Determination of 2'-acetyl erythromycin and erythromycin in human tonsil tissue by HPLC with coulometric detection.
A simple and sensitive high-performance liquid chromatographic method was developed for the determination of 2'-acetyl erythromycin and erythromycin in human tonsil tissue. Methyl tert-butyl ether was used as the extraction solvent after alkalization of tissue homogenates. Separation was achieved on a reverse phase C-18 column. The mobile phase consisted of acetonitrile-methanol-tetrahydrofuran-sodium acetate buffer, pH 4.5. Eluted compounds were monitored by a coulometric detector in the oxidative screen mode. The quantitation limits of 2'-acetyl erythromycin and erythromycin were 0.20 and 0.30 mg/kg of tissue, respectively. The method was linear over the concentration range of 0.60-10.0 mg/kg of tissue for 2'-acetyl erythromycin and erythromycin.
Haataja
H
H
Research Center, Orion Pharmaceutica, Espoo, Finland.
Kokkonen
P
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D014066:Palatine Tonsil / Q000032:analysis*; D011355:Prodrugs / Q000378:metabolism
D011355:Prodrugs; D004917:Erythromycin; C056625:erythromycin 2'-acetate