title: Chromatofocusing in the purification of staphylococcal enterotoxin D.
abstract: A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.
authors.affiliation: Food Research Institute, University of Wisconsin, Madison 53706.
authors.forename: Z
authors.initials: Z
authors.lastname: Lei
authors.forename: R F
authors.initials: RF
authors.lastname: Reiser
authors.forename: M S
authors.initials: MS
authors.lastname: Bergdoll
mesh_terms: D002850:Chromatography, Gel; D004768:Enterotoxins / Q000302:isolation & purification*
chemical_list: D004768:Enterotoxins; C019368:enterotoxin D, Staphylococcal