Characterization of phage-specific transfer RNA molecules coded by Vibrio eltor phage e4.
Transfer RNAs were isolated from phage e4-infected Vibrio eltor Mak 757 cells. These were aminoacylated with 14 individual 3H-labeled L-amino acids. Hybridization of these [3H]aminoacyl-tRNAs with phage e4 DNA revealed that the phage e4 encodes tRNAs for arginine, tryptophan, tyrosine, leucine, and isoleucine. Direct aminoacylation of phage-coded tRNA molecules isolated from phage DNA-RNA hybrids also confirmed this observation.
Chattopadhyay
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S
Indian Institute of Chemical Biology, Calcutta.
Ghosh
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RK
D001435:Bacteriophages / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D012347:RNA, Transfer, Arg / Q000235:genetics; D012355:RNA, Transfer, Ile / Q000235:genetics; D012356:RNA, Transfer, Leu / Q000235:genetics; D012364:RNA, Transfer, Trp / Q000235:genetics; D012365:RNA, Transfer, Tyr / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D014733:Vibrio
D012347:RNA, Transfer, Arg; D012355:RNA, Transfer, Ile; D012356:RNA, Transfer, Leu; D012364:RNA, Transfer, Trp; D012365:RNA, Transfer, Tyr; D012367:RNA, Viral; D012343:RNA, Transfer