Immobilized wheat germ agglutinin separates small oligosaccharides derived from poly-N-acetyllactosaminoglycans of embryonal carcinoma cells.
Five pure oligosaccharides derived from poly-N-acetyllactosaminoglycans of teratocarcinoma cells were chromatographed on immobilized wheat germ agglutinin (WGA). Three of them, Gal beta 1-4GlcNAc, GlcNAc beta 1-3Gal, and GlcNAc beta 1-3Gal beta 1-4GlcNAc, revealed only weak binding, but GlcNAc beta 1-6Gal beta 1-4GlcNAc was bound moderately and GlcNAc beta 1-6Gal was bound quite strongly. The differences in the binding affinities were large enough to allow chromatographic separation of the five oligosaccharides into three distinct fractions in a small column of WGA-agarose.
Renkonen
O
O
Department of Biochemistry, University of Helsinki, Finland.
Mäkinen
P
P
Hård
K
K
Helin
J
J
Penttilä
L
L
D000606:Amino Sugars / Q000032:analysis*; D000818:Animals; D002847:Chromatography, Agarose; D051379:Mice; D009844:Oligosaccharides / Q000302:isolation & purification*; D011134:Polysaccharides / Q000032:analysis*; D013724:Teratoma / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000032:analysis; D014909:Wheat Germ Agglutinins
D000606:Amino Sugars; D009844:Oligosaccharides; D011134:Polysaccharides; D014909:Wheat Germ Agglutinins