Simultaneous determination of R- and S-prenylamine in plasma and urine by reversed-phase high-performance liquid chromatography.
A high-performance liquid chromatographic method for the determination of R- and S-prenylamine in human plasma and urine is described. It involves a two-step liquid-liquid extraction of prenylamine from biological material and preparation of diastereomeric urea derivatives with R-(-)-naphthylethyl isocyanate, a chiral fluorescence marker. Separation and quantitation of the diastereomeric prenylamine derivatives are carried out by a reversed-phase high-performance liquid chromatographic system with fluorimetric detection. The limit of determination is less than 2 ng of enantiomer per ml of urine and less than 1 ng of enantiomer per ml of plasma. A preliminary kinetic study on one healthy volunteer who had received a single oral dose of racemic prenylamine (100-mg film tablet) showed distinctly higher plasma and urine concentrations of the R-enantiomer.
Gietl
Y
Y
Pharmakologisches Institut für Naturwissenschaftler der Johann Wolfgang Goethe-Universität, Frankfurt am Main, F.R.G.
Spahn
H
H
Mutschler
E
E
D000284:Administration, Oral; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D011299:Prenylamine / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D011299:Prenylamine