Comparison among five mutagenicity assays in workers producing polyurethane foams.
Thirty-two male individuals exposed to isocyanates and amines during the production of plastic foams and 20 male referents were studied by cytogenetic methods (chromosomal aberrations, sister chromatid exchanges and micronuclei in lymphocytes) and by urinary mutagenic assays (thioether concentrations and mutagenic activity with Salmonella TA98 and E. Coli WP2 uvrA). The occupational exposure was monitored by measurements of toluene diisocyanate and N-methylmorpholine in work-room air. The levels were below the current Swedish hygienic standards. Although all parameters, except the sister chromatid exchanges, showed increased mean values for the exposed group compared to the referents, only the urinary thioether concentrations differed significantly. The study was, however, non-conclusive with regard to a genetic effect of the occupational exposure as measured by the cytogenetic parameters. This may be due to the low exposure level. In the micronuclei frequencies there was a significant effect of age. Smoking significantly affected the SCE frequencies, the thioether concentrations and the mutagenic activities in the Salmonella assay. There were statistically significant correlations between the urine specimens collected during one working day and the following morning with regard to the mutagenic activities in the Salmonella and E. coli assays, and in the thioether concentrations as well. The association between the different cytogenetic and urinary mutagenic assays were weak but there were several statistically significant correlation coefficients, indicating that the variables may have a common metabolic background.
Holmén
A
A
Department of Occupational Medicine, Central Hospital, Halmstad, Sweden.
Akesson
B
B
Hansén
L
L
Frithiof
J
J
Mitelman
F
F
Karlsson
A
A
Persson
L
L
Welinder
H
H
Skerfving
S
S
Högstedt
B
B
D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis; D000588:Amines / Q000009:adverse effects* / Q000032:analysis; D002869:Chromosome Aberrations; D003485:Cyanates / Q000009:adverse effects* / Q000032:analysis; D004781:Environmental Exposure; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D011140:Polyurethanes; D012854:Sister Chromatid Exchange / Q000187:drug effects; D012907:Smoking / Q000009:adverse effects; D013440:Sulfides / Q000652:urine
D000395:Air Pollutants, Occupational; D000588:Amines; D003485:Cyanates; D009153:Mutagens; D011140:Polyurethanes; D013440:Sulfides; C005057:isocyanic acid