Variation in intramembranous particle distribution in the cell membrane of the sperm head of the plains rat and western chestnut mouse (Pseudomys Spp.).
Freeze-fracture studies were used to investigate the size, density, and distribution of intramembranous particles (IMPs) in the plasma membrane of the sperm head of two species of Australian hydromyine rodents Pseudomys australis and P. nanus both of which are characterized by having, in addition to a dorsal hook, two long ventral hooks that extend from the antero-concave surface. It was found that on the P face of the plasma membrane over the dorsal hook of caput and upper corpus spermatozoa a paracrystalline arrangement of small, approximately 7nm, IMPs were interspersed with a few large, 9 to 12 nm, IMPs. In the cauda epididymidis the small particles became randomly arranged. On the P face of the plasma membrane over the ventral hooks of caput sperm, and in the postacrosomal region, a much higher density of large, 9 to 12 nm, IMPs was present. In the ventral hooks of cauda sperm the IMPs were increased even further and were arranged in short, variably orientated, ridges; this did not appear to be evident in the plasma membrane over the postacrosomal region. It suggests a change in distribution of the IMPs in the cell membrane over the ventral hooks, but not over the postacrosomal region, during epididymal transit of the spermatozoa. This may indicate that the plasma membrane of these two regions of the head of ejaculated spermatozoa has intrinsic differences in its structure and function.
Breed
W G
WG
Department of Anatomy, University of Adelaide, Australia.
Swift
J G
JG
Mukherjee
T M
TM
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D005614:Freeze Fracturing / Q000379:methods; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D009115:Muridae / Q000033:anatomy & histology*; D051381:Rats; D013077:Sperm Head / Q000648:ultrastructure*; D013083:Sperm Transport; D013094:Spermatozoa / Q000648:ultrastructure*
D008562:Membrane Glycoproteins; D008565:Membrane Proteins