Detection of cellular retinol-binding protein messenger RNA in the somatic cells of the rat seminiferous tubules.
A cDNA clone coding for Cellular Retinol-Binding Protein (CRBP) was used as a probe to study the expression of the gene in the somatic cells of the seminiferous tubules (Sertoli and peritubular cells). In this paper we demonstrate that these cells are actively involved in the synthesis of the specific mRNA. In Sertoli cells the gene is modulated by the hormones effective in spermatogenesis, such as FSH and testosterone. Moreover, peritubular cells revealed an approximately two times higher concentration of CRBP steady-state mRNA levels when compared with Sertoli cells.
Galdieri
M
M
Istituto di Istologia ed Embriologia Generale, Università di Roma, La Sapienza.
Faraonio
R
R
Colantuoni
V
V
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012177:Retinol-Binding Proteins / Q000235:genetics*; D054840:Retinol-Binding Proteins, Cellular; D012671:Seminiferous Tubules / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
D012333:RNA, Messenger; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D004247:DNA