Cloning of a full-length complementary DNA for fatty-acid-binding protein from bovine heart.
A full-length cDNA for bovine heart fatty-acid-binding protein (H-FABP) was cloned from a lambda gt11 cDNA library established from bovine heart muscle. The cDNA sequence shows an open reading frame coding for a protein with 133 amino acids. Colinearity with the amino acid sequences of four tryptic peptides was asserted. H-FABP isolated from bovine heart begins with an N-acetylated valine residue, however, as derived from analysis of the tryptic, amino-terminal-blocked peptide and the molecular mass of the peptide obtained via secondary-ion mass spectrometry. The molecular mass of the total protein is 14673 Da. Bovine H-FABP is 89% homologous to rat H-FABP and 97% homologous to the bovine mammary-derived growth-inhibition factor described recently by Böhmer et al. [J. Biol. Chem. 262, 15137-15143 (1987)]. Significant homologies were also found with bovine myelin protein P2 and murine adipocyte protein p422. Secondary-structure predictions were proposed for these proteins, based on computer analysis, which reveal striking similarities.
Billich
S
S
Institut für Biochemie, Universität Münster, Federal Republic of Germany.
Wissel
T
T
Kratzin
H
H
Hahn
U
U
Hagenhoff
B
B
Lezius
A G
AG
Spener
F
F
D000107:Acetylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D003201:Computers; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010455:Peptides / Q000032:analysis; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010455:Peptides; D012333:RNA, Messenger; D004247:DNA