title: Canine distemper virus (CDV) immune-stimulating complexes (Iscoms), but not measles virus iscoms, protect dogs against CDV infection.
abstract: The potential of immune-stimulating complexes (iscoms), a novel form of antigenic presentation, for the induction of protective immunity against morbillivirus infection was shown by immunizing dogs with canine distemper virus (CDV) iscoms, which contained the fusion (F) protein and a minor amount of the haemagglutinin of the virus. The immunized dogs developed CDV-neutralizing antibodies but, in contrast to non-immunized dogs, did not develop viraemia or clinical signs of infection upon intranasal challenge with the virulent Snyder Hill strain of CDV. Immunization of dogs with measles virus (MV) iscoms, prepared either from affinity-purified MV F protein or from purified whole virus, resulted in partial protection against challenge with CDV. The data presented clearly show that the iscom form of antigenic presentation may be considered a serious candidate for subunit vaccines against morbillivirus infection.
authors.affiliation: Department of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: De Vries
authors.forename: F G
authors.initials: FG
authors.lastname: Uytdehaag
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D002499:Centrifugation, Density Gradient; D003429:Cross Reactions; D004216:Distemper / Q000517:prevention & control*; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008459:Measles virus / Q000276:immunology*; D008823:Micelles; D008854:Microscopy, Electron; D014611:Vaccination / Q000662:veterinary; D014760:Viral Fusion Proteins / Q000276:immunology; D014765:Viral Vaccines; D014766:Viremia / Q000517:prevention & control / Q000662:veterinary
chemical_list: D000276:Adjuvants, Immunologic; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008823:Micelles; D014760:Viral Fusion Proteins; D014765:Viral Vaccines