Detection of biotinylated nucleic acid hybrids by antibody-coated gold colloid.
A simple and sensitive nonradioactive method for detecting biotinylated nucleic acid hybrids on nitrocellulose paper is described. The method employs anti-biotin antibody-coated gold colloid and silver enhancement. Comparative results indicate sensitivity similar to that of a commonly used enzymatic colorimetric detection method.
Tomlinson
S
S
Molecular Diagnostics Inc., Miles Research Center, West Haven, Connecticut 06516.
Lyga
A
A
Huguenel
E
E
Dattagupta
N
N
D000345:Affinity Labels; D000906:Antibodies; D001710:Biotin / Q000276:immunology; D003101:Collodion; D003102:Colloids; D004247:DNA / Q000032:analysis; D006046:Gold; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D010316:Particle Size
D000345:Affinity Labels; D000906:Antibodies; D003102:Colloids; D009696:Nucleic Acids; D001710:Biotin; D006046:Gold; D003101:Collodion; D004247:DNA