Interaction of cibacron blue and anilinonaphthalenesulphonate with lipoproteins provides a new means for simple isolation of these plasma proteins.
The selective interaction of serum proteins with immobilized Cibacron Blue and the binding properties of the dye anilinonaphthalenesulphonate has been used to separate albumin and lipoproteins by affinity chromatography. The novel binding of anilinonaphthalenesulphonate to lipoproteins from the sera of lamprey, fish and mammals provides a simple procedure for the isolation of these plasma proteins, and permit preparation of specific antisera, tools particularly relevant for evolutionary and clinical studies.
Amthauer
R
R
Instituto de Bioquimica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
Concha
M
M
Villanueva
J
J
Krauskopf
M
M
D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology*; D000818:Animals; D002645:Chickens; D005399:Fishes; D006801:Humans; D007798:Lampreys; D008074:Lipoproteins / Q000302:isolation & purification*; D008722:Methods; D011817:Rabbits; D014227:Triazines / Q000494:pharmacology*
D000817:Anilino Naphthalenesulfonates; D008074:Lipoproteins; D014227:Triazines; C012842:Cibacron Blue F 3GA