Chromatofocusing in the purification of staphylococcal enterotoxin D.
A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.
Lei
Z
Z
Food Research Institute, University of Wisconsin, Madison 53706.
Reiser
R F
RF
Bergdoll
M S
MS
D002850:Chromatography, Gel; D004768:Enterotoxins / Q000302:isolation & purification*
D004768:Enterotoxins; C019368:enterotoxin D, Staphylococcal