title: Sequences contained within the promoter of the human thymidine kinase gene can direct cell-cycle regulation of heterologous fusion genes.
abstract: Recent evidence on the transcriptional regulation of the human thymidine kinase (TK) gene raises the possibility that cell-cycle regulatory sequences may be localized within its promoter. A hybrid gene that combines the TK 5' flanking sequence and the coding region of the bacterial neomycin-resistance gene (neo) has been constructed. Upon transfection into a hamster fibroblast cell line K12, the hybrid gene exhibits cell-cycle-dependent expression. Deletion analysis reveals that the region important for cell-cycle regulation is within -441 to -63 nucleotides from the transcriptional initiation site. This region (-441 to -63) also confers cell-cycle regulation to the herpes simplex virus thymidine kinase (HSVtk) promoter, which is not expressed in a cell-cycle manner. We conclude that the -441 to -63 sequence within the human TK promoter is important for cell-cycle-dependent expression.
authors.affiliation: Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033.
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Wells
authors.forename: Y F
authors.initials: YF
authors.lastname: Lau
authors.forename: A S
authors.initials: AS
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D002453:Cell Cycle; D003001:Cloning, Molecular; D006801:Humans; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D013937:Thymidine Kinase