title: Autoradiographic localization of vasopressin and oxytocin binding sites in rat kidney.
abstract: The presence of vasopressin receptors of the V1 (vascular) type and of oxytocin receptors in the rat kidney was investigated using an autoradiographical approach. Rat kidney sections were incubated with tritiated vasopressin ([3H]vasopressin, 1.5 nM) or oxytocin ([3H]oxytocin, 3 nM). The ligand selectivity of the [3H]vasopressin binding sites detected was deduced from competition experiments using one selective unlabeled ligand for V2 (antidiuretic) vasopressin receptors (1-deamino-[8-D-arginine]-vasopressin, dDAVP) and one selective unlabeled ligand for V1 receptors (des-glycineamide-[1-(beta-mercapto-beta,beta-cyclopentamethylene propionic acid]-arginine vasopressin, des(Gly(NH2)9d(CH2)5-AVP). Specific and dense [3H]vasopressin labeling was observable in the medullopapillary and cortical portions of the kidney. Specific [3H]vasopressin binding in the cortex was insensitive to the V1-selective ligand, des(Gly(NH2)9d(CH2)5-AVP, but was inhibited by dDAVP. Glomerular structures identified as such by microscopical observation of the kidney sections were specifically labeled with [3H]oxytocin and [125I]-SAR1-angiotensin II but not with [3H]vasopressin. It is concluded that V1 receptors which have been evidenced on mesangial cells in culture are not expressed in a detectable quantity on mesangial cells in situ. The specific [3H]oxytocin binding to glomeruli might reflect the presence on glomerular structures of oxytocin receptors involved in the effects of the hormone on renal hemodynamics, and possibly in some of the effects ascribed to vasopressin.
authors.affiliation: Departement de Physiologie, Centre Médical Universitaire, Geneva, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Tribollet
authors.forename: C
authors.initials: C
authors.lastname: Barberis
authors.forename: J J
authors.initials: JJ
authors.lastname: Dreifuss
authors.forename: S
authors.initials: S
authors.lastname: Jard
mesh_terms: D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D007668:Kidney / Q000378:metabolism*; D008297:Male; D010121:Oxytocin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014667:Vasopressins / Q000378:metabolism*
chemical_list: D014667:Vasopressins; D010121:Oxytocin