D-3-hydroxybutyrate metabolism in the perfused rat heart.
The utilization of D-3-HB and the production of acetoacetate by the perfused rat heart were investigated over a wide range of DL-3-HB concentrations. The rate of D-3-HB utilization is concentration dependent, and shows saturation kinetics. The oxidized amount of D-3-HB when D-3-HB as a sole substrate, accounts at a maximum for 50% of the total oxygen consumption, which suggest the contribution of the endogenous substrate as fuel source along with D-3-HB. The proportion of the D-3-HB consumed that is oxidized rather than released as acetoacetate increases from 70% to 93% as the concentration of D-3-HB falls from 6.99 mM to 0.30 mM.
Sultan
A M
AM
Department of Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000096:biosynthesis; D000818:Animals; D006885:Hydroxybutyrates / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D000090:Acetoacetates; D006885:Hydroxybutyrates; D020155:3-Hydroxybutyric Acid