7-ketocholesterol inhibits VLDL secretion by cultured human and rabbit hepatocytes.
7-ketocholesterol, one of the major product of autoxidation of dietary cholesterol, was found to inhibit secretion of very low density lipoprotein [14C]cholesterol, [14C]triacylglycerol and [35S]apoprotein B,E,C by cultured human and rabbit hepatocytes. A parallel inhibition (about 35%) of cholesterol synthesis but not of triacylglycerol formation was observed. Incubation with 10 micrograms/ml of oxysterol also reduced the total apo-B secretion measured by ELISA and increased intracellular apo-B mRNA level. These results seem to indicate that 7-ketocholesterol decreases secretion of very low density lipoprotein (VLDL) particles and exerts inhibitory effects on apo-B production at the co-translational or posttranslational level.
Kosykh
V A
VA
U.S.S.R. Cardiology Research Center, Academy of Medical Sciences, Moscow.
Podres
E A
EA
Sudarickov
A B
AB
Berestetskaya
Y V
YV
Surguchov
A P
AP
Repin
V S
VS
Smirnov
V N
VN
D000328:Adult; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics; D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives*; D006801:Humans; D007653:Ketocholesterols / Q000494:pharmacology*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
D001055:Apolipoproteins B; D007653:Ketocholesterols; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger; D002784:Cholesterol; D008715:Methionine; C003001:7-ketocholesterol