Potassium permanganate used to eliminate interference with the Porter-Silber reaction in the butanol extraction method for determination of urinary 17-hydroxycorticosteroids.
We describe a simple, rapid, and less interference-susceptible method for using the Porter-Silber reaction for determination of urinary 17-hydroxycorticosteroids. The procedure is based on butanol extraction of the steroids after the urine sample is treated with potassium permanganate and sodium bisulfite. These treatments, together with an additional acid- and alkali-washing of the extract, could eliminate most of the substances in urine that interfere with the Porter-Silber reactions. Values so obtained correlated well (r = 0.95) with those by Furuya's method in which beta-glucuronidase is used. Our method may be useful and suitable for a screening test of adrenocortical and pituitary functions.
Maeda
T
T
Central Clinical Laboratories, Tokyo Metropolitan Police Hospital, Japan.
Matsuzaki
H
H
Sekine
T
T
D020001:1-Butanol; D015065:17-Hydroxycorticosteroids / Q000652:urine*; D000303:Adrenal Cortex Diseases / Q000175:diagnosis; D000440:Butanols; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010084:Oxidation-Reduction; D010900:Pituitary Diseases / Q000175:diagnosis; D011196:Potassium Permanganate; D013053:Spectrophotometry
D015065:17-Hydroxycorticosteroids; D000440:Butanols; D011196:Potassium Permanganate; D020001:1-Butanol