Identification of a cDNA clone for sorghum leaf malate dehydrogenase (NADP). Light-dependent mRNA accumulation.
The mechanisms underlying the photoregulation of the synthesis of sorghum leaf malate dehydrogenase (NADP) (EC 1.1.1.82) (NADP-MDH), a key enzyme in C4 photosynthesis, have been investigated. During the greening process a light-dependent increase in enzyme activity took place, accompanied by de novo synthesis of the protein. In vitro translation experiments showed that this chloroplastic protein is synthesized as a precursor (46 kDa) with a 'transit peptide' of about 2.5 kDa. A large increase in NADP-MDH-translatable RNAs was also observed during greening. We describe also the construction and characterization of a cDNA clone for NADP-MDH (pCM18A) in the expression vector lambda gt11. The use of this homologous probe demonstrated a light-dependent mRNA accumulation.
Crétin
C
C
Laboratoire de Physiologie Végétale Moléculaire, Unité Associée 1128, Université Paris-Sud, Orsay, France.
Luchetta
P
P
Joly
C
C
Miginiac-Maslow
M
M
Decottignies
P
P
Jacquot
J P
JP
Vidal
J
J
Gadal
P
P
D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D008027:Light; D008291:Malate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D010944:Plants / Q000201:enzymology*; D014176:Protein Biosynthesis / Q000528:radiation effects; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000528:radiation effects
D012333:RNA, Messenger; D004247:DNA; D008291:Malate Dehydrogenase