Rapid identification of color additives, using the C18 cartridge: collaborative study.
Nine laboratories collaboratively studied a method for the separation and identification of the 7 permitted FD&C color additives (Red Nos. 3 and 40; Blue Nos. 1 and 2; Yellow Nos. 5 and 6; Green No. 3) and the banned FD&C Red No. 2 in foods. The method is based on use of a commercial C18 cartridge and spectrophotometry or thin layer chromatography. Collaborators analyzed 5 commercial products (noodles, candy, carbonated soda, flavored gelatin, and powdered drink) and 2 dye mixtures (one containing FD&C Red Nos. 2, 3, and 40; the other containing FD&C Green No. 3 and Red No. 3). All of the colors were identified with little or no difficulty by 8 collaborators. The method has been adopted official first action.
Young
M L
ML
Food and Drug Administration, New York Regional Laboratory, Brooklyn 11232.
D002855:Chromatography, Thin Layer; D005505:Food Coloring Agents / Q000032:analysis*; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
D005505:Food Coloring Agents; D007202:Indicators and Reagents