Lipid-protein interaction. The incorporation of myelin proteolipid apoprotein into phosphatidylcholine bilayers.
Bovine myelin proteolipid apoprotein (PLA), obtained in high yield and purity by a novel ultrafiltration procedure, has been used to study the perturbations produced by this protein on phosphatidylcholine bilayers, using infrared spectroscopy, nuclear magnetic resonance and fluorescence polarisation. PLA interacts with phospholipids in a similar manner to other intrinsic proteins. For bilayers in the fluid state, the fatty-acyl chain static order, as measured by deuterium NMR, is slightly increased in the presence of the protein, except at very high PLA concentrations. Phosphorus NMR reveals some perturbation of the phospholipid polar group by PLA, but to a smaller degree than occurs with other intrinsic proteins. An increase in static order above tc (the onset temperature for gel-to-fluid transition) is also detected by infrared spectroscopy. Studies using steady-state polarisation of diphenylhexatriene fluorescence indicate that the microviscosity of the bilayer increases as a function of the protein mole fraction. From these data an estimation of the average number of lipids perturbed per protein monomer has been made, and a figure of 37 phospholipid molecules determined. The data are compatible with a picture of a hydrophobic polypeptide, perturbing the phospholipids close to it, but allowing rapid (greater than 10(4) s-1) exchange with all the lipid molecules in the system.
Goñi
F M
FM
Department of Biochemistry, University of the Basque Country, Bilbao, Spain.
Cózar
M
M
Alonso
A
A
Durrani
A A
AA
García-Segura
L M
LM
Lee
D C
DC
Monreal
J
J
Chapman
D
D
D001059:Apoproteins / Q000378:metabolism*; D003903:Deuterium; D004591:Electrophoresis, Polyacrylamide Gel; D005454:Fluorescence Polarization; D005614:Freeze Fracturing; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes / Q000032:analysis / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009185:Myelin Proteins / Q000378:metabolism*; D018991:Myelin Proteolipid Protein; D010713:Phosphatidylcholines; D010758:Phosphorus; D013055:Spectrophotometry, Infrared
D001059:Apoproteins; D008051:Lipid Bilayers; D008081:Liposomes; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D010713:Phosphatidylcholines; D010758:Phosphorus; D003903:Deuterium