Isolation and partial characterization of a bacteriophage active on Hyphomicrobium sp. WI-926.
Isolation of a Hyphomicrobium phage from raw sewage from Athens, Ohio, was achieved by a combination of differential centrifugation, filtration, enrichment in mixed Hyphomicrobium cultures, and purification on individual host strains by subculturing single plaques in soft agar overlayers. Enrichments with water from Lake Erie and Lake Beechwood (Ohio) were unsuccessful. Out of 21 Hyphomicrobium strains and 22 other Gram-negative and Gram-positive bacteria tested, only Hyphomicrobium WI-926 (isolated from a German forest pond) was susceptible. This phage had an isometric head (diameter between opposite apices, 67 nm) and a short (12 nm), noncontractile tail and belongs thus to the morphogroup C1. It contained double-stranded DNA. The single-step growth curve showed a latent period of 9 h, a rise period of 6 h, and a burst size of 35. The various differentiation stages in the host development exhibited different affinities for phage adsorption and development. While all stages allowed phage adsorption, the daughter cells were most efficient. Phage multiplication was limited to daughter cells, and the development of infected swarmer cells was arrested permanently at this stage.
Preissner
W C
WC
Department of Zoological and Biomedical Sciences, Ohio University, Athens 45701.
Maier
S
S
Völker
H
H
Hirsch
P
P
D001419:Bacteria / Q000254:growth & development* / Q000648:ultrastructure; D018407:Bacterial Physiological Phenomena; D001435:Bacteriophages / Q000302:isolation & purification* / Q000648:ultrastructure; D008854:Microscopy, Electron