Cloning and expression of human nebulin cDNAs and assignment of the gene to chromosome 2q31-q32.
We have isolated two nonoverlapping cDNAs encoding human nebulin, a muscle-specific protein. Northern hybridization analysis shows that nebulin is encoded by a huge message at least 25 kb in length. By hybridizing two nonoverlapping cDNAs to DNA isolated from rodent X human cell hybrids, we assign this presumably single-copy gene to human chromosome 2; sublocalization studies indicate that the nebulin gene is on the long arm of the chromosome, in the region 2q31-q32.
Zeviani
M
M
H. Houston Merritt Clinical Research Center for Muscular Dystrophy and Related Disorders, Columbia University, New York, New York 10032.
Darras
B T
BT
Rizzuto
R
R
Salviati
G
G
Betto
R
R
Bonilla
E
E
Miranda
A F
AF
Du
J
J
Samitt
C
C
Dickson
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D011817:Rabbits
D009124:Muscle Proteins; C035620:nebulin; D004247:DNA