Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene.
We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.
Feinbaum
R L
RL
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Ausubel
F M
FM
D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004790:Enzyme Induction / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic
D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA; D000217:Acyltransferases; C020141:flavanone synthetase