title: Gas chromatographic studies of the carbamylation of haemoglobin by methyl isocyanate in rats and rabbits.
abstract: Carbamylation of the N-terminal valine of haemoglobin with methyl isocyanate in rats and rabbits has been demonstrated in vitro and in vivo by gas chromatography. N-Methylcarbamylated haemoglobin, converted by cyclization into 3-methyl-5-isopropylhydantoin, has been quantified by gas chromatography. Standard hydantoin was synthesized, chemically characterized and used for calibration. The method is simple and reliable in the concentration range 0.06-2 nmol. Carbamylation of haemoglobin by methyl isocyanate in vivo in rats can be identified only above a dose of 1.05 mg/l in inhalation exposures. It is inferred that methyl isocyanate in the "active" form crosses the alveolar and erythrocyte membranes and carbamylates the haemoglobin.
authors.affiliation: Defence Research and Development Establishment, Gwalior, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Ramachandran
authors.forename: B R
authors.initials: BR
authors.lastname: Gandhe
authors.forename: K S
authors.initials: KS
authors.lastname: Venkateswaran
authors.forename: M P
authors.initials: MP
authors.lastname: Kaushik
authors.forename: R
authors.initials: R
authors.lastname: Vijayaraghavan
authors.forename: G S
authors.initials: GS
authors.lastname: Agarwal
authors.forename: N
authors.initials: N
authors.lastname: Gopalan
authors.forename: M V
authors.initials: MV
authors.lastname: Suryanarayana
authors.forename: S K
authors.initials: SK
authors.lastname: Shinde
authors.forename: S
authors.initials: S
authors.lastname: Sriramachari
mesh_terms: D000818:Animals; D000986:Antisickling Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002849:Chromatography, Gas; D003485:Cyanates / Q000138:chemical synthesis / Q000494:pharmacology*; D006454:Hemoglobins / Q000032:analysis*; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D017953:Isocyanates; D011817:Rabbits; D051381:Rats
chemical_list: D000986:Antisickling Agents; D003485:Cyanates; D006454:Hemoglobins; D017953:Isocyanates; C008461:methyl isocyanate