Metabolism of diltiazem. I. Structures of new acidic and basic metabolites in rat, dog and man.
The metabolites of diltiazem in the urine of rats, dogs and man and rat bile were investigated. New metabolites including six acidic metabolites (A1-A6) and four basic metabolites (M9, MB, MC, MD) were isolated by high performance liquid chromatography and their structures were characterized by gas chromatography-mass spectrometry. Acidic metabolites A1, A2, A3, A4 and basic metabolite MD identified by comparing their properties with the synthetic compounds; (+)-(2S, 3S)-2-(4-methoxyphenyl)-3-acetoxy-4-oxo-2,3,4,5-tetrahydro-1,5- benzothiazepin-5-acetic acid (A1), 3-deacetyl-A1 (A2), O-demethyl-A1 (A3), O-demethyl-3-deacetyl-A1 (A4) and N-didemethyl-diltiazem (MD). All acidic metabolites have a CH2COOH group which may be formed by oxidative deamination of the CH2CH2N(CH3)2 group of diltiazem.
Sugawara
Y
Y
Biological Research Laboratory, Tanabe Seiyaku Co., Saitama, Japan.
Ohashi
M
M
Nakamura
S
S
Usuki
S
S
Suzuki
T
T
Ito
Y
Y
Kume
T
T
Harigaya
S
S
Nakao
A
A
Gaino
M
M
D000818:Animals; D001646:Bile / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004110:Diltiazem / Q000378:metabolism* / Q000652:urine; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012588:Scintillation Counting; D013045:Species Specificity
D004110:Diltiazem