Ethanol increases synaptosomal free calcium concentration.
The effect of ethanol on synaptosomal free calcium concentration [( Ca2+]i) and 45Ca2+ uptake was studied in vitro. Synaptosomes were prepared from forebrains of adult rats and incubated in the presence of ethanol 50-500 mM. [Ca2+]i determined by means of the Ca2+-sensitive dye, fura-2, was increased by ethanol in polarized and depolarized synaptosomes, whereas 45Ca2+ uptake was decreased. The results suggest that ethanol may influence neuronal calcium homeostasis by increasing rather than decreasing [Ca2+]i.
Davidson
M
M
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Wilce
P
P
Shanley
B
B
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism
D000431:Ethanol; D002118:Calcium