High-performance liquid chromatographic determination of formate as benzimidazole in biological samples.
Formate was determined as benzimidazole by high-performance liquid chromatography after reaction with o-phenylenediamine at 130 degrees C for 2 h in 1 M perchloric acid. The useful concentration range was 1.6-40 mumol/l and the determination limit was 20 pmol. The recoveries from rat liver homogenate and human urine were 90.3 +/- 2.9 and 89.4 +/- 2.5%, respectively. Using this method, the activity of formaldehyde dehydrogenase in biological samples could be measured, and also the formate concentration in the liver and urine of rats to which methanol had been administered.
Ohmori
S
S
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Sumii
I
I
Toyonaga
Y
Y
Nakata
K
K
Kawase
M
M
D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification; D000818:Animals; D001562:Benzimidazoles / Q000302:isolation & purification*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D005561:Formates / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D008099:Liver / Q000032:analysis / Q000201:enzymology; D008297:Male; D000432:Methanol / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013552:Swine
D001562:Benzimidazoles; D005561:Formates; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases; D000432:Methanol