title: Automated measurement of kidney lysosomes by light microscopy. Influence of cyclosporine treatment.
abstract: After staining for acid phosphatase, video images were acquired from 1-micron sections of the kidneys of rats treated with cyclosporine. Automated segmentation of the lysosomes was followed by measurements of their area, number and optical density; tubular area was delineated manually. In addition to small lysosomes, representing more than 80% of the total number, all kidneys contained a second population of larger organelles. Cyclosporine treatment with 50 mg/kg/day for one week and with 25 mg/kg/day for three weeks, but not with 25 mg/kg/day for one week, induced a significant increase of lysosomal area, especially in the larger organelles (+200%). In a few animals, this was accompanied by a reduction in the number of lysosomes, suggesting a fusion of the organelles. Other parameters were not consistently modified by treatment. Cyclosporine treatment also elicited the appearance of fat droplets; however, lysosomes were not enlarged in tubular cells containing fat droplets.
authors.affiliation: Department of Human Anatomy, Free University of Brussels Faculty of Medicine and Pharmacy, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Coen
authors.forename: F
authors.initials: F
authors.lastname: Roels
authors.forename: A
authors.initials: A
authors.lastname: Cornelis
authors.forename: A
authors.initials: A
authors.lastname: Claeys
authors.forename: G A
authors.initials: GA
authors.lastname: Verpooten
authors.forename: M E
authors.initials: ME
authors.lastname: De Broe
mesh_terms: D000818:Animals; D003524:Cyclosporins / Q000633:toxicity*; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D050356:Lipid Metabolism; D008247:Lysosomes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014741:Video Recording
chemical_list: D003524:Cyclosporins