title: Regulation of alternatively spliced alpha-tropomyosin gene expression by nerve extract.
abstract: Muscle cells were incubated with extracts prepared from neural tissue to investigate the mechanisms and factors which regulate mRNA transcript production of the alternatively spliced alpha-tropomyosin (alpha-TM) gene. Results from RNA analyses demonstrate that alpha-TM transcripts are dramatically altered by decreasing the production of the striated specific isoform while concomitantly increasing a cytoplasmic isoform. The factors which regulate alpha-TM expression are derived from neural tissue and act at the post-transcriptional level through a trans-activation mechanism. Furthermore, these factors can also alter the expression of alpha-TM mRNA isoforms in both non-muscle and primary muscle cells by regulating the exon splice site selection determined by endogenous trans-acting factors and force the production of a specific cytoplasmic isoform. The results demonstrate that the neural factors which regulate alpha-TM expression act through mechanisms and processes which are common to many different cell types and cell lineages.
authors.affiliation: Department of Microbiology & Molecular Genetics, University of Cincinnati College of Medicine, Ohio 45267-0524.
authors.forename: D F
authors.initials: DF
authors.lastname: Wieczorek
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D002460:Cell Line; D004274:DNA, Recombinant / Q000378:metabolism*; D005091:Exons; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes; D009132:Muscles; D009928:Organ Specificity; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013116:Spinal Cord / Q000502:physiology*; D014020:Tissue Extracts / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014335:Tropomyosin / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D012333:RNA, Messenger; D014020:Tissue Extracts; D014335:Tropomyosin