Removal of contaminating nucleoside diphosphates from commercial preparations of uridine diphosphoglucose.
Nucleoside diphosphate contamination in commercial preparations of UDP-Glc poses potential problems in activity assays for enzymes that use this substrate. For removing these contaminants, we report a simple, inexpensive, and rapid method that obviates the need for uncertain corrections in assay calculations.
Outlaw
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WH
Department of Biological Science, Florida State University, Tallahassee 32306-3050.
Hite
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DR
Fiore
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D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D009702:Nucleoside Diphosphate Sugars / Q000302:isolation & purification*; D014532:Uridine Diphosphate Glucose / Q000032:analysis*; D014539:Uridine Diphosphate Sugars / Q000032:analysis*
D009702:Nucleoside Diphosphate Sugars; D014539:Uridine Diphosphate Sugars; D014532:Uridine Diphosphate Glucose