Autosomal recessive polycystic kidney disease in a murine model. A gross and microscopic description.
The C57BL/6J-cpk genetic murine model of autosomal recessive polycystic kidney disease was examined to gain insight into the pathogenesis of renal cystic disease. Fetal through 3-week-old offspring of heterozygote matings were used to study growth parameters and morphology of this genetic form of cystic disease. The kidneys were examined by light and electron microscopy and nephron segments were microdissected. Two phases of cystic disease development were morphologically identified. The first phase in fetal and newborn affected pups was characterized by proximal tubule enlargement and a general increase in the tubular mitotic index. The proximal tubules showed cytologic abnormalities along with an increased necrotic cell index. The later phase, in one through 3-week-old cystic pups, was characterized by progressive enlargement of the kidneys due mainly to cystic change of the collecting ducts and by development of azotemia. Secondary to the azotemia was a stunted body growth. Significant tubular epithelial hyperplasia was not found by mitotic index during the second phase, but an increase in collecting duct cellularity was present. Histone H4 gene expression, which is tightly coupled to DNA synthesis and thus an index of cell proliferation, showed only a minimal increase in cystic kidneys at 1, 2, and 3 weeks of age. Therefore, the degree of cell proliferation necessary to allow the observed tubular enlargement appears to be minimal.
Gattone
V H
VH
Department of Anatomy, University of Kansas Medical Center, Kansas City.
Calvet
J P
JP
Cowley
B D
BD
Evan
A P
AP
Shaver
T S
TS
Helmstadter
K
K
Grantham
J J
JJ
D000818:Animals; D000831:Animals, Newborn; D001806:Blood Urea Nitrogen; D001835:Body Weight; D002455:Cell Division; D004195:Disease Models, Animal; D006657:Histones / Q000235:genetics; D007668:Kidney / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008940:Mitotic Index; D009929:Organ Size; D007690:Polycystic Kidney Diseases / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D014511:Uremia / Q000097:blood
D006657:Histones; D012333:RNA, Messenger