Analysis of human vaginal secretions by SDS-polyacrylamide gel electrophoresis.
Proteins in 68 vaginal secretions from 50 women were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Although there were considerable numbers of intermediate types, the secretions could be classified into the following groups based on the densitometric patterns. A relatively low concentration of 67K protein (albumin) and a high concentration of 52K protein (IgG) were the patterns common to pre- and post-menstrual phases and pregnant stadium. A relatively high concentration of the 67K protein and a moderate concentration of the 52K protein characterized the midcycle. A considerably high concentration of 67K protein compared with the 79K (transferrin), 58K (IgA) and 52K bands was the pattern for menstrual phase and postpartum stage.
Itoh
Y
Y
Department of Forensic Medicine, School of Medicine, Juntendo University, Tokyo, Japan.
Manaka
M
M
D000293:Adolescent; D000328:Adult; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D006801:Humans; D008597:Menstrual Cycle; D008970:Molecular Weight; D049590:Postpartum Period / Q000378:metabolism; D011247:Pregnancy / Q000378:metabolism; D011506:Proteins / Q000032:analysis*; D014621:Vagina / Q000378:metabolism*
D011506:Proteins