[Production and characterization of monoclonal antibodies to the secretory components of human IgA].
The monoclonal antibodies BL-HSC/1-3 are characterized by means of indirect radioimmunotechnique with regard to their binding pattern to a set of human proteins. The results suggest a binding specificity of the three monoclonals for A-determinants of the human secretory component. However, we could not point out a different binding pattern to the immunoglobulin-bound and the free molecule, respectively. By use of appropriate immunoassays, the monoclonal antibody BL-HSC/3 is the most suitable one for the detection of the secretory component and secretory IgA in body fluids. The protein band labeled by BL-HSC/3 in the immunoblotting analysis corresponds to a molar mass of 79,000 D according to the data known for the human secretory component.
Seidel
B
B
Bereich Tierphysiologie und Immunbiologié, Karl-Marx-Universität Leipzig.
Hädge
D
D
Eichler
W
W
Fiebig
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003126:Colostrum / Q000276:immunology; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D013280:Stomatitis / Q000276:immunology
D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory
Herstellung und Charakterisierung von monoklonalen Antikörpern gegen die sekretorische Komponente des humanen IgA.