Computerized image-analysis microspectroscopy of tissue sections.
A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.
Pappolla
M A
MA
Laboratory Service, Montrose Veterans Administration Medical Center, NY 10548.
D000818:Animals; D002531:Cerebellum / Q000166:cytology; D046508:Culture Techniques; D007091:Image Processing, Computer-Assisted; D008853:Microscopy; D051381:Rats; D013057:Spectrum Analysis