Determination of gamma-aminobutyric acid and associated amino acids in mouse brain by liquid chromatography.
A rapid method is described for the analysis of amino acids containing in mouse brain by high performance liquid chromatography equipped with a fluorescence detector. Pre-column o-phthalaldehyde-2-mercaptoethanol derivatives of the amino acids were injected onto an ODS C-18 column. 5-Amino-n-valeric acid, a non-endogenous amino acid, was used as an internal standard. Separation and elution of amino acid derivatives were achieved by varying the ratio of organic phase by a step gradient. Significant increases in gamma-aminobutyric acid, glutamine and glycine levels in the brain were detected 2 h after injection of 800 mg/kg valproic acid (VPA). A high dose administration of VPA was correlated with Reye's syndrome.
Miyazaki
C
C
Department of Hospital Pharmacy, Nagasaki University Hospital, Japan.
Ogasawara
M
M
Ichikawa
M
M
Matsuyama
K
K
Goto
S
S
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*; D009764:o-Phthalaldehyde
D000596:Amino Acids; D007202:Indicators and Reagents; D005680:gamma-Aminobutyric Acid; D009764:o-Phthalaldehyde