title: Mechanism of biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme.
abstract: The biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme was investigated by 13C nuclear magnetic resonance spectroscopy of the oxoacid formed from 13C-labeled acetate by washed suspensions. The threonine pathway could be excluded, and the results are in accord with a mechanism for the formation of 2-oxobutyrate from acetyl coenzyme A and pyruvate via citramalate.
authors.affiliation: Department of Biology, University of Oslo, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Nesbakken
authors.forename: P
authors.initials: P
authors.lastname: Kolsaker
authors.forename: J
authors.initials: J
authors.lastname: Ormerod
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D045782:Hemiterpenes; D007651:Keto Acids / Q000096:biosynthesis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy
chemical_list: D045782:Hemiterpenes; D007651:Keto Acids; C016211:alpha-keto-beta-methylvaleric acid; D000105:Acetyl Coenzyme A; C001505:alpha-ketoisovalerate; C013082:alpha-ketoisocaproic acid