Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 2. Incorporation of the light-driven proton pump bacteriorhodopsin.
A method has been developed for identifying the step in a detergent-mediated reconstitution procedure at which an integral membrane protein can be associated with phospholipids to give functional proteoliposomes. Large liposomes prepared by reverse-phase evaporation were treated with various amounts of the detergents Triton X-100, octyl glucoside, or sodium cholate as described in the preceding paper [Paternostre, M.-T., Roux, M., & Rigaud, J. L. (1988) Biochemistry (preceding paper in this issue)]. At each step of the solubilization process, we added bacteriorhodopsin, the light-driven proton pump from Halobacterium halobium. The protein-phospholipid detergent mixtures were then subjected to SM2 Bio-Beads treatments to remove the detergent, and the resulting vesicles were analyzed with respect to protein insertion and orientation in the membrane by freeze-fracture electron microscopy, sucrose density gradients, and proton pumping measurements. The nature of the detergent used for reconstitution proved to be important for determining the mechanism of protein insertion. With sodium cholate, proteoliposomes were formed only from ternary phospholipid-protein-detergent micelles. With octyl glucoside, besides proteoliposome formation from ternary mixed micelles, direct incorporation of bacteriorhodopsin into preformed liposomes destabilized by saturating levels of this detergent was observed and gave proteoliposomes with optimal proton pumping activity. With Triton X-100, protein insertion into destabilized liposomes was also observed but involved a transfer of the protein initially present in phospholipid-Triton X-100-protein micelles into Triton X-100 saturated liposomes. Our results further demonstrated that protein orientation in the resulting proteoliposomes was critically dependent upon the mechanism by which the protein was incorporated.
Rigaud
J L
JL
Département de Biologie, CEN Saclay, Gif-sur-Yvette, France.
Paternostre
M T
MT
Bluzat
A
A
D001436:Bacteriorhodopsins / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003902:Detergents; D008081:Liposomes; D008565:Membrane Proteins; D008954:Models, Biological; D017830:Octoxynol; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D011510:Proteolipids; D013501:Surface-Active Agents
D002250:Carbon Radioisotopes; D003902:Detergents; D008081:Liposomes; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D011510:Proteolipids; D013501:Surface-Active Agents; C020485:proteoliposomes; D011092:Polyethylene Glycols; D001436:Bacteriorhodopsins; D017830:Octoxynol