title: Reliable diagnosis of Trichosomoides crassicauda in the urinary bladder of the rat.
abstract: Two reliable methods are described for identifying infection of laboratory rats with the nematode Trichosomoides crassicauda. The first is a rapid method where cryostat sections of the rat urinary bladder are stained with acridine orange and viewed under a fluorescence microscope. The second involves the stabilization of the bladder surface prior to examination using scanning electron microscopy (SEM).
authors.affiliation: Department of Medicine, University of Auckland, New Zealand.
authors.forename: J
authors.initials: J
authors.lastname: Cornish
authors.forename: M A
authors.initials: MA
authors.lastname: Vanderwee
authors.forename: G
authors.initials: G
authors.lastname: Findon
authors.forename: T E
authors.initials: TE
authors.lastname: Miller
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009348:Nematoda / Q000302:isolation & purification* / Q000648:ultrastructure; D009349:Nematode Infections / Q000175:diagnosis / Q000662:veterinary*; D051381:Rats / Q000469:parasitology*; D012376:Rodent Diseases / Q000175:diagnosis*; D001743:Urinary Bladder / Q000469:parasitology / Q000648:ultrastructure; D001745:Urinary Bladder Diseases / Q000175:diagnosis / Q000662:veterinary*; D014556:Urine / Q000469:parasitology