The hybrid EIA test: a specific and sensitive assay for the detection of woodchuck antibody to hepatitis surface antigen (anti-WHs).
'Ausria II' polystyrene beads (Abbott Labs, N. Chicago) are reacted with woodchuck serum positive for WHsAg in a dilution predetermined by titration. This modified bead is used in a blocking assay to detect the presence of antibody to the surface antigen of woodchuck hepatitis virus (anti-WHs). Serum containing woodchuck anti-WHs and commercial horseradish peroxidase (HRP) labeled anti-HBs are sequentially added. A drop in optical density at 492 nm of 50% or more due to the blocking of HRP conjugated anti-HBs by anti-WHs compared with a control (negative woodchuck serum) is a measure of anti-WHs. The ease and simplicity of converting readily available 'Ausria II' beads to specific reagents for detecting anti-WHs should be welcomed by investigators studying WHV. The method described is both sensitive and reproducible.
Millman
I
I
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Glass
R G
RG
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000954:Antigens, Surface / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006518:Hepatitis Viruses / Q000276:immunology*; D008392:Marmota / Q000276:immunology*; D012589:Sciuridae / Q000276:immunology*
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral