title: Growth and macromolecular content of the dimorphic fungus Aureobasidium pullulans and the effect of hydroxyurea and other inhibitors.
abstract: The growth kinetics and the macromolecular content of the yeast and ethanol-induced hyphal forms of Aureobasidium pullulans were studied. During the morphological transition from yeasts to hyphae, both the protein and RNA content decreased significantly, the mycelial form containing only 76% of the amount of protein in the yeasts, and 38% of the RNA. The DNA was the only component tested whose level increased during the transition. Among several compounds inhibiting macromolecular synthesis, only hydroxyurea showed a remarkable effect on the morphology of A. pullulans, inducing the mycelial morphology. The macromolecular composition of hydroxyurea-treated cultures changed with time in a way similar to that of the ethanol-Tween 80-ammonia medium, and to that of carbon-starved cultures, without ethanol or glucose.
authors.affiliation: Departamento de Biología, Facultad de Ciencias, Universidad del País Vasco, Bilbao, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Sevilla
authors.forename: M D
authors.initials: MD
authors.lastname: Moragues
authors.forename: F
authors.initials: F
authors.lastname: Uruburu
mesh_terms: D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis* / Q000096:biosynthesis; D000431:Ethanol / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis* / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D006918:Hydroxyurea / Q000494:pharmacology*; D007700:Kinetics; D003904:Mitosporic Fungi / Q000032:analysis / Q000187:drug effects / Q000254:growth & development*; D011136:Polysorbates / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012331:RNA, Fungal / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D011136:Polysorbates; D000644:Quaternary Ammonium Compounds; D012331:RNA, Fungal; D000431:Ethanol; D005947:Glucose; D006918:Hydroxyurea