title: Quantitative distribution of muscarinic receptors and choline acetyltransferase in rat medulla: examination of transmitter-receptor mismatch.
abstract: Recent reports have identified discrepancies between the anatomical distribution of transmitters and their receptors, a phenomenon known as transmitter-receptor mismatch. However, quantitative determinations of transmitter activity and receptor density in individual brain regions have not been conducted in parallel. We therefore sought to determine quantitatively the relationship between muscarinic acetylcholine receptor density and the density of cholinergic innervation as reflected by activity of the biosynthetic enzyme for acetylcholine, choline acetyltransferase (ChAT). To assure sampling of equivalent regions using the two methods, an 'electronic micropunch' technique was developed to allow measurement of [3H]quinuclidinyl benzilate ([3H]QNB) binding within the corresponding cylinders of tissue obtained by the micropunch cannula. Nineteen regions of the rat medulla (1 mm diameter, 1 mm height) were studied. The micropunch region containing the gracile nucleus, the area postrema and the choroid plexus of the fourth ventricle contained the highest ChAT activity, but exhibited little [3H]QNB binding to muscarinic receptors. However, among the remaining 18 regions a strong correlation was obtained between uncorrected muscarinic receptor density and ChAT activity within each micropunched region (r = 0.89, n = 18). Correction for autoradiographic efficiency weakened the overall relationship between receptor density and ChAT activity (r = 0.58, n = 18). This was due to a relatively high density of receptors associated with fiber tract regions containing low ChAT activity. The presence of receptors within white matter is ordinarily obscured by high tritium quenching. This is consistent with the hypothesis that a portion of muscarinic receptors are located extrasynaptically and may be present within axons.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Neurobiology, Cornell University Medical College, New York, NY 10021.
authors.forename: P
authors.initials: P
authors.lastname: Ernsberger
authors.forename: S P
authors.initials: SP
authors.lastname: Arneric
authors.forename: V
authors.initials: V
authors.lastname: Arango
authors.forename: D J
authors.initials: DJ
authors.lastname: Reis
mesh_terms: D000818:Animals; D001345:Autoradiography; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D002799:Cholinergic Fibers / Q000032:analysis*; D008526:Medulla Oblongata / Q000032:analysis*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011976:Receptors, Muscarinic / Q000032:analysis*
chemical_list: D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D002795:Choline O-Acetyltransferase