A possible role for altered poly(adenosine diphosphoribose)-synthesis in the sensitivity of human head and neck squamous carcinoma cells to ionizing radiation.
Cytotoxicity, extent of DNA double-strand breaks, and stimulation of poly(adenosine diphosphoribose)-synthesis were measured in two established human head and neck squamous carcinoma cell lines (183A and 1483) following x-irradiation. The 1483 cell line was 15-fold more resistant to x-ray-mediated cytotoxicity than was the 183A cell line. X-ray-mediated DNA strand cleavage also differed in these two cell lines with the absolute frequency of DNA double-strand breaks in the sensitive cells 183A cells being twice that in the resistant 1483 cell line. No detectable stimulation of poly(adenosine diphosphoribose)-synthesis was measured in the sensitive 183A cells whereas a marked increase in incorporation of [3H]-nicotinamide adenine dinucleotide was readily detected following x-irradiation of the resistant 1483 cells. These findings suggest a possible role of altered poly(adenosine diphosphoribose)-synthesis in the sensitivity of human head and neck squamous carcinoma cells to ionizing radiation.
Wassermann
K
K
Department of Medical Oncology, University of Texas M. D. Anderson Hospital and Tumor Institute, Houston.
Newman
R A
RA
McLaughlin
J D
JD
Sacks
P G
PG
Zwelling
L A
LA
D002294:Carcinoma, Squamous Cell; D002455:Cell Division / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D006258:Head and Neck Neoplasms; D006801:Humans; D009702:Nucleoside Diphosphate Sugars / Q000528:radiation effects*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis / Q000528:radiation effects*; D014965:X-Rays
D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose