Nucleotide sequence of the celC gene encoding endoglucanase C of Clostridium thermocellum.
The nucleotide sequence of the cellulase gene celC, encoding endoglucanase C of Clostridium thermocellum, has been determined. The coding region of 1032 bp was identified by comparison with the N-terminal amino acid (aa) sequence of endoglucanase C purified from Escherichia coli. The ATG start codon is preceded by an AGGAGG sequence typical of ribosome-binding sites in Gram-positive bacteria. The derived amino acid sequence corresponds to a protein of Mr 40,439. Amino acid analysis and apparent Mr of endoglucanase C are consistent with the amino acid sequence as derived from the DNA sequencing data. A proposed N-terminal 21-aa residue leader (signal) sequence differs from other prokaryotic signal peptides and is non-functional in E. coli. Most of the protein bears no resemblance to the endoglucanases A, B, and D of the same organism. However, a short region of homology between endoglucanases A and C was identified, which is similar to the established active sites of lysozymes and to related sequences of fungal cellulases.
Schwarz
W H
WH
Institute for Microbiology, Technical University of Munich, F.R.G.
Schimming
S
S
Rücknagel
K P
KP
Burgschwaiger
S
S
Kreil
G
G
Staudenbauer
W L
WL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics*; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D003062:Codon; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D003062:Codon; D021382:Protein Sorting Signals; D002480:Cellulase