The effect of fast protein liquid chromatography (FPLC) cationic exchange purification on the crystallization of a monoclonal Fab fragment.
Fast protein liquid chromatography cationic exchange purification to homogeneity of a monoclonal Fab fragment has resulted in an improvement in the quality of crystals for X-ray diffraction studies. This improvement is displayed in a well-defined crystal morphology, reproducibility of crystal growth, and increased resolution of diffraction data.
Orbell
J D
JD
St. Vincent's Institute of Medical Research, Melbourne, Victoria, Australia.
Guddat
L W
LW
Machin
K J
KJ
Isaacs
N W
NW
D002852:Chromatography, Ion Exchange; D003460:Crystallization; D007140:Immunoglobulin Fab Fragments / Q000032:analysis*; D014961:X-Ray Diffraction
D007140:Immunoglobulin Fab Fragments