Serum binding of indapamide in health and disease: primary role of alpha 1-acid glycoprotein.
The serum concentrations of alpha-1-acid glycoprotein (AAG), albumin (HSA), and non-esterified fatty acids (NEFA), and the serum binding of indapamide were measured in four groups of individuals: control (healthy) subjects (N = 24), patients with inflammatory syndrome (N = 28), with hepatic (N = 20) and renal (N = 27) insufficiency. Indapamide serum binding was increased in patients with inflammatory syndrome (82.2 +/- 3.4%, P less than .001), decreased in patients with hepatic insufficiency (72.3 +/- 5.9%, P less than .001) and unchanged in patients with renal insufficiency (77.7 +/- 2.8%) as compared with controls (78.2 +/- 3.1%). A multivariate analysis indicated that these changes were mainly related to concomitant changes in AAG concentration (that explained 63% of intersubject variability in bound/free binding ratio), and to a lesser extent to HSA (that explained only 4% of the variability in the binding). These data show that the free fraction of the acidic drug indapamide in serum is affected by pathologic conditions in which changes in AAG concentration occur and that, unexpectedly, HSA plays a negligible role in the binding.
Urien
S
S
Laboratoire de Pharmacologie, Faculté de Médecine, Créteil, France.
Morin
D
D
d'Athis
P
P
Coulomb
B
B
Tillement
J P
JP
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism; D004232:Diuretics / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D006801:Humans; D007190:Indapamide / Q000097:blood*; D007249:Inflammation / Q000097:blood; D007674:Kidney Diseases / Q000097:blood; D008107:Liver Diseases / Q000097:blood; D008297:Male; D008875:Middle Aged; D009961:Orosomucoid / Q000378:metabolism*; D011485:Protein Binding; D012737:Sex Factors
D004232:Diuretics; D005230:Fatty Acids, Nonesterified; D009961:Orosomucoid; D007190:Indapamide