Larval development and metamorphosis of the American lobster Homarus americanus (Crustacea, Decapoda): effect of eyestalk ablation and juvenile hormone injection.
Removal of eyestalks of Homarus americanus on different days and molting stages during larval development revealed that eyestalk tissue is involved in the larval and postlarval molting rhythm and in preparation for metamorphosis as early as the end of Stage II. Eyestalk removal in stages II and III reduced the duration of larval and postlarval stages. Eyestalk removal up to the end of Stage II delayed the completion of metamorphosis by one or two molts and caused additional development stages (designated IVa, IV', and V'). In this study, the critical stage for eyestalk ablation to delay metamorphosis occurred at the end of molt stage D1 of larval Stage II (the seventh day of development at 20 degrees). Injection of juvenile hormone before the critical stage resulted in a few intermediate stage IV' animals. This study demonstrates the involvement of eyestalk neuroendocrine tissue in the control of metamorphosis and investigates a possible involvement of juvenile hormone.
Charmantier
G
G
Laboratoire de Physiologie des Invertébrés, Université des Scienceset Techniques du Languedoc, Montpellier, France.
Charmantier-Daures
M
M
Aiken
D E
DE
D000818:Animals; D005260:Female; D007273:Injections, Intramuscular / Q000379:methods; D007605:Juvenile Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D008675:Metamorphosis, Biological / Q000187:drug effects*; D008121:Nephropidae / Q000502:physiology*; D009799:Ocular Physiological Phenomena
D007605:Juvenile Hormones