title: Ethanol increases synaptosomal free calcium concentration.
abstract: The effect of ethanol on synaptosomal free calcium concentration [( Ca2+]i) and 45Ca2+ uptake was studied in vitro. Synaptosomes were prepared from forebrains of adult rats and incubated in the presence of ethanol 50-500 mM. [Ca2+]i determined by means of the Ca2+-sensitive dye, fura-2, was increased by ethanol in polarized and depolarized synaptosomes, whereas 45Ca2+ uptake was decreased. The results suggest that ethanol may influence neuronal calcium homeostasis by increasing rather than decreasing [Ca2+]i.
authors.affiliation: Department of Biochemistry, University of Queensland, St. Lucia, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Davidson
authors.forename: P
authors.initials: P
authors.lastname: Wilce
authors.forename: B
authors.initials: B
authors.lastname: Shanley
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000431:Ethanol; D002118:Calcium