title: Regulation of tubulin and actin synthesis and accumulation during Blastocladiella emersonii development.
abstract: Actin and alpha and beta-tubulin have been identified in Blastocladiella emersonii by two-dimensional gel electrophoresis and Western blotting. The kinetics of synthesis of these proteins were compared by pulse-labeling experiments with [35S]methionine and with the accumulation of their corresponding mRNAs, translated in a cell-free system. Large increases occur in the rates of actin and alpha- and beta-tubulin biosynthesis during sporulation and there is an accumulation of the corresponding mRNAs. In parallel to the increased synthesis, these cytoskeletal proteins accumulate during the late stage of sporulation.
authors.affiliation: Departamento de Bioquímica, Universidade de São Paulo, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: da Silva
authors.forename: M H
authors.initials: MH
authors.lastname: Juliani
mesh_terms: D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D001754:Blastocladiella / Q000378:metabolism / Q000502:physiology*; D008411:Chytridiomycota / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D013172:Spores, Fungal; D014404:Tubulin / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin