Non-competitive enzyme-linked immunosorbent assay for human apolipoprotein SAA or S.
Purified antibodies against human apolipoprotein S were used to measure apo S levels in human plasma by a sandwich ELISA technique. The method developed was highly sensitive and specific. A reliable calibration of a plasma standard was obtained by reassociating the purified protein with plasma HDL. A significant correlation (r = 0.69) was found between plasma apo S and CRP values. This correlation coefficient reached 0.97 when the plasma samples were selected according to additional clinical and laboratory criteria.
Dubois
D Y
DY
Research Foundation on Atherosclerosis, Brussels, Belgium.
Malmendier
C L
CL
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001053:Apolipoproteins / Q000032:analysis* / Q000276:immunology / Q000592:standards; D001667:Binding, Competitive; D002097:C-Reactive Protein / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007115:Immunization Schedule; D008075:Lipoproteins, HDL / Q000032:analysis* / Q000276:immunology / Q000592:standards; D011817:Rabbits; D012015:Reference Standards; D000685:Serum Amyloid A Protein / Q000032:analysis* / Q000276:immunology / Q000592:standards
D001053:Apolipoproteins; D007106:Immune Sera; D008075:Lipoproteins, HDL; D000685:Serum Amyloid A Protein; C029798:apolipoprotein S-peptides; D002097:C-Reactive Protein