title: Analytical performance of three commercially available nephelometers compared for quantifying proteins in serum and cerebrospinal fluid.
abstract: We investigated the analytical performance and imprecision of three commercially available nephelometers for the quantification of various proteins in pooled serum and cerebrospinal fluid. Albumin, transferrin, IgG, IgA, and IgM in serum were determined nephelometrically (BNA, Behring; Array, Beckman) and turbidimetrically (Cobas Bio, Hoffmann-La Roche). All these proteins in cerebrospinal fluid except transferrin were determined nephelometrically with all three systems. CVs and lower limits of detection were compared for all instruments, and stability of calibration curves was evaluated.
authors.affiliation: Department of Clinical Chemistry, Maasland Hospital, Sittard, The Netherlands.
authors.forename: H J
authors.initials: HJ
authors.lastname: Salden
authors.forename: B M
authors.initials: BM
authors.lastname: Bas
authors.forename: I T
authors.initials: IT
authors.lastname: Hermans
authors.forename: P C
authors.initials: PC
authors.lastname: Janson
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D006801:Humans; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods
chemical_list: D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins