Dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against dengue.
A cDNA copy of the dengue (DEN) 2 virus genome region encoding the virion capsid, membrane and envelope structural proteins has been inserted into vaccinia virus (VV) DNA under the control of its 11K late promoter. The DEN-2 envelope protein was expressed and processed in cells infected with the VV recombinant (VV/D2S). No DEN-2 virus antibody response was detected in mice, hamsters or monkeys vaccinated with VV/D2S. Furthermore, a viraemia was observed in recombinant-vaccinated monkeys after challenge with infectious DEN-2 virus.
Deubel
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V
Division of Vector-Borne Viral Diseases, Centers for Disease Control, Fort Collins, Colorado 80522.
Kinney
R M
RM
Esposito
J J
JJ
Cropp
C B
CB
Vorndam
A V
AV
Monath
T P
TP
Trent
D W
DW
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D003062:Codon / Q000235:genetics; D006224:Cricetinae; D004279:DNA, Viral / Q000235:genetics; D003715:Dengue / Q000517:prevention & control*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D005260:Female; D007118:Immunoassay; D008252:Macaca fascicularis; D051379:Mice; D009693:Nucleic Acid Hybridization; D014158:Transcription, Genetic; D014614:Vaccines, Synthetic; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines; D014766:Viremia / Q000517:prevention & control
D000914:Antibodies, Viral; D000956:Antigens, Viral; D003062:Codon; D004279:DNA, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines