Peripheral blood leukocyte count as an index of defense status in the leukopenic host.
These experimental studies have investigated the reliability of the peripheral blood leukocyte count to predict whether the leukopenic host can contain or eliminate infection. Additionally, we have investigated the possibility that determination of leukocyte recruitment, supplementary to peripheral blood leukocyte counts, might allow individuals with neutropenia at risk from serious infection to be distinguished with greater certainty. Varying doses of radiation, cyclophosphamide, and methylprednisolone were used to induce distinct levels of leukopenia in rats. Leukocyte recruitment was measured by quantifying the response of neutropenic animals to evocative, subcutaneous stimuli, and the results of this assay were then compared with circulating leukocyte counts in the same individuals. Six models of experimentally induced infection were used to compare circulating and recruitable leukocytes as indicators of the susceptibility of the leukopenic host to infection. Response curves relating leukocyte numbers to host resistance were similar when circulating or recruitable leukocytes were used as an index of defense capability. These findings support the use of peripheral blood leukocyte numbers as an index of resistance to infection in individuals with leukopenia and suggest that functional analyses such as leukocyte recruitment are unlikely to provide additional information.
Cawley
S
S
Department of Medicine, Auckland Hospital, New Zealand.
Findon
G
G
Miller
T E
TE
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005260:Female; D007239:Infections / Q000097:blood; D007958:Leukocyte Count / Q000187:drug effects / Q000528:radiation effects; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D007970:Leukopenia / Q000097:blood*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology; D051381:Rats
D003520:Cyclophosphamide; D008775:Methylprednisolone