Crystallization of halophilic malate dehydrogenase from Halobacterium marismortui.
Malate dehydrogenase from the extreme halophile Halobacterium marismortui crystallizes in highly concentrated phosphate solution in space group 12 with cell dimensions a = 113.8 A, b = 122.8 A, c = 126.7 A, beta = 98.1 degrees. The halophilic enzyme was found to be unstable at lower concentrations of phosphate. It associates with unusually large amounts of water and salt, and the combined particle volume shows a tight fit in the unit cell.
Harel
M
M
Department of Structural Chemistry, Weizmann Institute of Science, Rehovot, Israel.
Shoham
M
M
Frolow
F
F
Eisenberg
H
H
Mevarech
M
M
Yonath
A
A
Sussman
J L
JL
D006217:Halobacterium / Q000201:enzymology*; D008291:Malate Dehydrogenase; D014961:X-Ray Diffraction
D008291:Malate Dehydrogenase