Influence of two glutamine-containing dipeptides on growth of mammalian cells.
The instability of the amino acid glutamine prompted us to investigate substitute compounds appropriate for culture conditions. The effect of two glutamine-containing dipeptides, alanylglutamine (Ala-Gln) and glycylglutamine (Gly-Gln), on the growth behavior of a hematopoietic cell line in culture (K562) was investigated. Growth rates and [3H]thymidine incorporation rates of cells cultivated in sterile-filtrated media, containing glutamine (Gln) or Ala-Gln or Gly-Gln, were not statistically different. Although heart-sterilization of media containing Gln caused approximately 95% decomposition of the Gln, both dipeptides remained unaltered. Consequently, cell growth was drastically decreased when autoclaved free Gln-containing media were used, but growth was unaffected in the presence of autoclaved dipeptides. Both Ala-Gln and Gly-Gln have an advantage over free Gln as growth factors for cell culture due to the stability of the dipeptides during both autoclaving and storage; the biological activity, however, is comparable.
Roth
E
E
First Clinic of Surgery, Metabolic Research Laboratory, University of Vienna, Austria.
Ollenschlager
G
G
Hamilton
G
G
Simmel
A
A
Langer
K
K
Fekl
W
W
Jakesz
R
R
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004151:Dipeptides / Q000032:analysis / Q000494:pharmacology*; D005973:Glutamine / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006358:Hot Temperature; D013242:Sterilization
D003470:Culture Media; D004151:Dipeptides; D005973:Glutamine; C039788:glycylglutamine; D004247:DNA; C054122:alanylglutamine