Biosynthesis of glycosaminoglycans in the human colonic tumor cell line Caco-2: structural changes occurring with the morphological differentiation of the cells.
The human colon cancer cell line Caco-2 cultured in vitro displayed morphological differentiation which was shown to be a growth-related event. We have investigated this phenomenon further in relation to the cell surface glycosaminoglycans produced by growing (5-day, i.e., prior to differentiation) and confluent (9-day, i.e., after morphological and functional differentiation) cultures. Neosynthesized [35S]glycosaminoglycans were purified on DEAE-cellulose; at confluency, they were bound more strongly to the column than the corresponding fractions from the growing cells. Analysis of Kav values of heparan sulfate and chondroitin sulfates from growing and confluent cells indicated an increase in chain length of both glycosaminoglycans in morphologically differentiated cells. Heparan sulfate was the main 35S-labeled glycosaminoglycan of the cell surface of both 5-day and 9-day cultures. Paper chromatography of the unsaturated disaccharides obtained by chondroitinase digestion showed that chondroitin sulfate chains were primarily 6-sulfated in the 2 studied extracts. Heparan sulfate chains were isolated as chondroitinase-resistant material and treated with nitrous acid. Analysis of N- and O-sulfate group-related radioactivity showed an increase in the amount of 35S-label in the form of N-sulfate groups and an increase in the O-35S-sulfation pattern in heparan sulfate from morphologically differentiated cells. Thus, the structural features of both chondroitin sulfates and heparan sulfate were significantly different when the growing cells became morphologically differentiated.
Levy
P
P
Laboratoire de Biochimie, INSERM U 181, Faculté de Médecine Saint-Antoine, Paris, France.
Robert
A
A
Picard
J
J
D002454:Cell Differentiation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D006025:Glycosaminoglycans / Q000032:analysis / Q000096:biosynthesis*; D006497:Heparan Sulfate / Q000032:analysis; D006801:Humans; D014407:Tumor Cells, Cultured
D006025:Glycosaminoglycans; D006497:Heparan Sulfate