A comparative study of the effects of aging on the responsiveness of the cholinergic receptor of the isolated ileum of mouse and rat.
The responsiveness of the cholinergic receptor (ChR) of the isolated ileum of mouse and rat was characterized by determining the ED50 of acetylcholine (ACh) and pA2 of mepenzolate (MPZ) in tissues from animals of different ages. The ages (in months) of mice were 3, 6 and 18 and; 3, 6, 12 and 24 for rats respectively. The responsiveness of ChR in mice and rats decreased with age. There was no difference in the pA2 between the preparations derived from mice and rats nor was it affected by age. The results of this investigation suggest that (1) changes in the peripheral ChR with age may have potential in the understanding of central aging process and (2) the use of age rather than the weight of animal may provide reproducible data in studies using isolated preparations.
Ochillo
R F
RF
Laboratory of Pharmacology, Xavier University of Louisiana, New Orleans 70125.
Tsai
C S
CS
D000109:Acetylcholine / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000818:Animals; D007082:Ileum; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000502:physiology*
D011950:Receptors, Cholinergic; D000109:Acetylcholine