title: Possible regulation of cation-induced pinocytosis in Amoeba proteus by phospholipase A.
abstract: We have studied the effects of exogenous phospholipids and compounds which are known to alter the activity of phospholipase A (PLA) on Ca2+-dependent, Na+-induced pinocytosis in Amoeba proteus. The PLA-inhibitors mepacrine, p-bromophenacyl bromide (pBPB) and Rosenthal's inhibitor depressed pinocytosis. Normal pinocytotic intensity was restored by the addition of Ca2+ or picomolar concentrations of lysolecithin. Very low concentrations of lysophospholipids and different molecular species of lecithins increased the capacity for pinocytosis in starved amoebae. The effect of the lecithins but not of the corresponding lysolecithins was abolished by PLA-inhibitors. Also, the restoration of the pinocytotic capacity of starved amoebae by melittin and mastoparan, which are known to stimulate PLA, was inhibited by mepacrine and pBPB. Isolated amoeba plasma membranes contain phospholipase A1 and A2 activity and the amoebae secrete a lipid (PRF, pinocytosis regulating factor) which has lysolecithin-like effects on pinocytosis. The enzyme activities and the release of PRF were markedly decreased by the PLA-inhibitors. Our observations support the hypothesis that PRF is a lysophospholipid that may constitute a signal for the formation of pinocytotic channels in the initial stages of pinocytosis. The phospholipase A activity of the amoeba must therefore be assigned an important role in the regulation of the Ca2+-dependent, cation-induced pinocytosis.
authors.affiliation: Department of Pharmacology, University of Lund/Sweden.
authors.forename: J O
authors.initials: JO
authors.lastname: Josefsson
authors.forename: G
authors.initials: G
authors.lastname: Arvidson
authors.forename: P
authors.initials: P
authors.lastname: Cobbold
mesh_terms: D000656:Amoeba / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002412:Cations; D006706:Homeostasis; D007700:Kinetics; D010713:Phosphatidylcholines / Q000494:pharmacology; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000302:isolation & purification / Q000378:metabolism*; D054466:Phospholipases A1; D010873:Pinocytosis / Q000187:drug effects; D012964:Sodium / Q000494:pharmacology
chemical_list: D002412:Cations; D010713:Phosphatidylcholines; D012964:Sodium; D010740:Phospholipases; D010741:Phospholipases A; D054466:Phospholipases A1; D002118:Calcium