title: The effect of a plasma protein fraction on lipid synthesis in cultured skin fibroblasts from normals and patients with hyperapobetalipoproteinemia.
abstract: Lipid synthesis was measured in cultured skin fibroblasts obtained from normals and patients with Hyperapobetalipoproteinemia (HyperapoB). Using lipoprotein-deficient serum medium, triglyceride synthesis and cholesterol esterification were greater in normals than in HyperapoB due to differences in de novo synthesis, not to differences in re-esterification or to different rates of hydrolysis. When normal and HyperapoB cells were incubated in serum-free medium, however, lipid synthesis was the same. Serum was then fractionated chromatographically and a partially purified protein fraction shown to be responsible for the stimulatory effect in normals.
authors.affiliation: Cardiovascular Research Unit, Royal Victoria Hospital, Montreal, Quebec.
authors.forename: K
authors.initials: K
authors.lastname: Cianflone
authors.forename: M A
authors.initials: MA
authors.lastname: Rodriguez
authors.forename: M
authors.initials: M
authors.lastname: Walsh
authors.forename: H
authors.initials: H
authors.lastname: Vu
authors.forename: A D
authors.initials: AD
authors.lastname: Sniderman
mesh_terms: D000328:Adult; D001055:Apolipoproteins B / Q000097:blood*; D001798:Blood Proteins / Q000502:physiology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood*; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D012867:Skin / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D001055:Apolipoproteins B; D001798:Blood Proteins; D008055:Lipids; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides; D005947:Glucose