Rapid, simultaneous determination of isomers of retinal, retinal oxime and retinol by high-performance liquid chromatography.
Thirteen geometric isomers of retinol, retinal and retinal oxime were resolved in a single chromatographic run on two 250 X 4 mm 5-micron LiChrosorb Si 60 columns in series, using 11.2% ethyl acetate, 1.4% 1-octanol and 2% dioxane in hexane as the mobile phase. All the 11-cis and all-trans isomers of retinol and retinal oxime were completely resolved from each other and from the 9-cis and 13-cis isomers. This chromatographic procedure should be particularly useful for quantifying geometric isomers of retinoids in methylene chloride-hydroxylamine extracts of biological samples such as ocular tissues.
Landers
G M
GM
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Olson
J A
JA
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D007536:Isomerism; D008029:Lighting; D010091:Oximes / Q000032:analysis*; D012015:Reference Standards; D012160:Retina / Q000032:analysis; D012172:Retinaldehyde / Q000032:analysis*; D012176:Retinoids / Q000032:analysis*; D013237:Stereoisomerism; D014801:Vitamin A / Q000032:analysis*
D004224:Diterpenes; D010091:Oximes; D012176:Retinoids; D014801:Vitamin A; C030628:retinaloxime; D012172:Retinaldehyde