Functional properties of proteins immobilized on albumin microspheres.
Bovine serum albumin (BSA) microspheres with an average diameter of 12.5 micron were prepared by crosslinking of BSA molecules with glutaraldehyde in the presence of polymethylmethacrylate dissolved in chloroform-toluene. Trypsin and anti-human IgG antibody were immobilized onto their surfaces by the glutaraldehyde-activation method. The catalytic activity and storage stability of the immobilized trypsin were satisfactorily high. The enzyme immunoassay (EIA) method using BSA-microspheres as a solid phase has a high sensitivity (the minimum concentration of detectable antigen in the sample: 0.2 ng/ml) and a wide concentration range (final concentration 0.027-3000 ng/ml) for the detection of human IgG.
Yan
C H
CH
Department of Polymer Chemistry, Kyoto University, Japan.
Kitano
H
H
Ise
N
N
D004797:Enzyme-Linked Immunosorbent Assay; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007700:Kinetics; D008863:Microspheres; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D014357:Trypsin / Q000378:metabolism*
D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D014357:Trypsin