Potentiation of the anti-lipolytic effect of 2-chloroadenosine after chronic caffeine treatment.
The effect of chronic caffeine treatment on lipolysis in rat epididymal adipose tissue was studied. There was a decrease in body weight, epididymal fat pad weight and mean adipocyte diameter in caffeine-treated rats when compared with control rats. No difference in adipocyte triglyceride content or mean adipocyte weight between control and caffeine-treated rats was observed. Lipolysis in adipocytes induced by adenosine deaminase (1 U/ml) decreased by 35% in caffeine-treated rats. This was accompanied by a 2.5-fold increase in the anti-lipolytic potency of 2-chloroadenosine and an increase of adipocyte adenosine A1 receptor number.
Cheung
W T
WT
Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong.
Lee
C M
CM
Ng
T B
TB
D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002110:Caffeine / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D004357:Drug Synergism; D066298:In Vitro Techniques; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D014970:Xanthines / Q000494:pharmacology
D014970:Xanthines; D015762:2-Chloroadenosine; C027512:1,3-diethyl-8-phenylxanthine; D002110:Caffeine; D000241:Adenosine