title: Measurement in vitro of a larval migration inhibitory factor in gastrointestinal mucus of sheep made resistant to the roundworm Trichostrongylus colubriformis.
abstract: Lambs were challenged by dosing with 2500 T. colubriformis larvae per day for 34 weeks, rested for 4 weeks and then re-challenged with the infective larvae for a further 10 weeks. A technique for the measurement of inhibition of larval migration from agar gels was used to investigate the antiparasitic activity of mucus, obtained from the small intestine and abomasum of the lambs, at the end of the re-challenge period. Measurements were also made on ileal digesta samples collected at different times during the development of the initial resistance, the rest period and the re-challenge period, and on faeces collected during the re-challenge infection. The mucus from the small intestine and abomasum paralysed and inhibited larval migration from agar gels significantly more (P less than 0.01 and P less than 0.05, respectively) than corresponding mucus from parasite-free control animals. This inhibitory activity was also detected (P less than 0.01) in the ileal digesta of infected animals from Week 6 of primary dosing. The magnitude of the inhibition in the ileal digesta increased with time during the infection period and was again detected during re-infection (P less than 0.01). It was not detectable in resistant sheep towards the end of the rest period. Slight inhibitory activity was detected in faeces after 2 weeks of re-infection. These observations suggest that substances secreted into the lumen of the small intestine of infected animals are responsible for resistance against ingested larvae.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen, Gt. Britain.
authors.forename: A E
authors.initials: AE
authors.lastname: Kimambo
authors.forename: J C
authors.initials: JC
authors.lastname: MacRae
mesh_terms: D000018:Abomasum / Q000276:immunology / Q000469:parasitology; D000818:Animals; D005243:Feces / Q000276:immunology / Q000469:parasitology; D007082:Ileum / Q000276:immunology / Q000469:parasitology; D007113:Immunity, Innate; D007421:Intestine, Small / Q000276:immunology / Q000469:parasitology; D007814:Larva / Q000276:immunology; D008297:Male; D009093:Mucus / Q000276:immunology* / Q000469:parasitology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology*; D013997:Time Factors; D014252:Trichostrongyloidiasis / Q000662:veterinary*; D014253:Trichostrongylosis / Q000276:immunology / Q000662:veterinary*