The B-G region genes of the chicken MHC are responsible for lethal graft-versus-host disease in newly hatched chickens.
Using the genetic model of Prague recombinant congenic lines of chickens we found that incompatibility in the B-G region of the major histocompatibility complex (MHC) causes very severe graft-versus-host reaction (GVHR)-associated haemolytic anaemia in newly hatched chickens. Unexpectedly, mild or no signs of this GVH disease are elicited when a recipient chick and an adult donor of lymphocytes are incompatible in the whole B haplotype (B-F/L + B-G regions). On the other hand, the B-G region incompatibility alone (as has been described previously) is not sufficient to produce any GVH splenomegaly in embryos at 14 days of incubation. However, GVH splenomegaly in the donor-recipient combinations with the difference in the whole B haplotype (B-F/L + B-G regions) is significantly greater than in those with the B-F/L region difference only. These results confirm that the B-G region genes of chicken MHC are also involved in the histocompatibility reactions. Furthermore, a new hypothetical model for the structure of the chicken MHC is discussed.
Plachý
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
D000744:Anemia, Hemolytic, Autoimmune / Q000235:genetics; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D006086:Graft vs Host Disease / Q000235:genetics*; D006239:Haplotypes; D006650:Histocompatibility Testing; D008285:Major Histocompatibility Complex; D011995:Recombination, Genetic; D013163:Splenomegaly / Q000235:genetics