1,6-Dinitropyrene causes spindle disturbances and chromosomal damage in V79 Chinese hamster cells.
We have investigated the cytogenetic effect of 1,6-dinitropyrene (1,6-DNP) in Chinese hamster V79 cells. The chemical caused a dose-dependent increase in the incidence of initial and full C-mitoses, polyploid mitoses, ana-telophases with lagging chromosomes, non-disjunction and multipolar configurations, in a range of 0.05-5 microM. These findings indicate that 1,6-DNP interferes with the functioning of the spindle apparatus in V79 cells. Early signs of spindle disturbances were seen at 1,6-DNP concentrations which only moderately reduced cell growth and division. Analysis of structural chromosomal aberrations revealed the appearance of chromatid-type aberrations with open breaks and exchanges accompanied by gaps. The results indicate that 1,6-DNP is both a spindle-disturbing and a clastogenic agent in V79 cells.
Bauchinger
M
M
Institut für Strahlenbiologie, Umweltforschung Munich, Neuherberg, F.R.G.
Schmid
E
E
Wiebel
F J
FJ
Roscher
E
E
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects; D009153:Mutagens; D011721:Pyrenes / Q000633:toxicity*; D008941:Spindle Apparatus / Q000187:drug effects*
D009153:Mutagens; D011721:Pyrenes; C033309:1,6-dinitropyrene