title: Formation of gamma-amino-beta-hydroxybutyric acid from 2-hydroxyputrescine in rat brain.
abstract: gamma-Amino-beta-[3H]hydroxybutyric acid ([3H]-GABOB) was formed in rat brain from 2-[3H]-hydroxyputrescine that had been chemically synthesized from 2-oxoputrescine and [3H]sodium borohydride. After the injection of 2-[3H]hydroxyputrescine into the lateral ventricle of a rat brain, the rat was killed and then the brain was removed. [3H]GABOB in the brain was identified by a combination of ion-exchange chromatography, high-voltage paper electrophoresis, and recrystallization of the radioactive compound with authentic GABOB.
authors.affiliation: Department of Psychiatry, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Noto
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: J
authors.initials: J
authors.lastname: Nakao
authors.forename: H
authors.initials: H
authors.lastname: Kamimura
authors.forename: H
authors.initials: H
authors.lastname: Harada
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D004590:Electrophoresis, Paper; D008297:Male; D011700:Putrescine / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: C004480:4-amino-3-hydroxybutyric acid; C002952:2-hydroxyputrescine; D005680:gamma-Aminobutyric Acid; D011700:Putrescine