title: Molecular cloning of the cDNA for human erythrocyte beta-spectrin.
abstract: Overlapping cDNA clones, totaling 3.3 kilobases (kb) in length, which encode over 50% of the human erythrocyte beta-spectrin subunit, were isolated by antibody screening of a lambda gt11 expression library constructed from human fetal liver mRNA. The amino acid sequence of the C-terminus of beta-spectrin was derived. The size of beta-spectrin mRNA in human erythroleukemia cells was found to be 7.5 kb. Erythrocyte beta-spectrin is encoded by a gene located on human chromosome 14, as determined by cDNA hybridization to human X mouse somatic cell hybrids.
authors.affiliation: Department of Medicine, Yale University School of Medicine, New Haven, CT.
authors.forename: J C
authors.initials: JC
authors.lastname: Winkelmann
authors.forename: T L
authors.initials: TL
authors.lastname: Leto
authors.forename: P C
authors.initials: PC
authors.lastname: Watkins
authors.forename: R
authors.initials: R
authors.lastname: Eddy
authors.forename: T B
authors.initials: TB
authors.lastname: Shows
authors.forename: A J
authors.initials: AJ
authors.lastname: Linnenbach
authors.forename: K E
authors.initials: KE
authors.lastname: Sahr
authors.forename: N
authors.initials: N
authors.lastname: Kathuria
authors.forename: V T
authors.initials: VT
authors.lastname: Marchesi
authors.forename: B G
authors.initials: BG
authors.lastname: Forget
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D005796:Genes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D013049:Spectrin / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012333:RNA, Messenger; D013049:Spectrin; D004247:DNA