Molecular cloning of the cDNA for human erythrocyte beta-spectrin.
Overlapping cDNA clones, totaling 3.3 kilobases (kb) in length, which encode over 50% of the human erythrocyte beta-spectrin subunit, were isolated by antibody screening of a lambda gt11 expression library constructed from human fetal liver mRNA. The amino acid sequence of the C-terminus of beta-spectrin was derived. The size of beta-spectrin mRNA in human erythroleukemia cells was found to be 7.5 kb. Erythrocyte beta-spectrin is encoded by a gene located on human chromosome 14, as determined by cDNA hybridization to human X mouse somatic cell hybrids.
Winkelmann
J C
JC
Department of Medicine, Yale University School of Medicine, New Haven, CT.
Leto
T L
TL
Watkins
P C
PC
Eddy
R
R
Shows
T B
TB
Linnenbach
A J
AJ
Sahr
K E
KE
Kathuria
N
N
Marchesi
V T
VT
Forget
B G
BG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D005796:Genes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D013049:Spectrin / Q000235:genetics* / Q000302:isolation & purification
D012333:RNA, Messenger; D013049:Spectrin; D004247:DNA