[Protein synthesis and antibody production in hybridoma cells under hyperthermia].
The relation between rates of protein synthesis and antibody production was studied for hybridoma cells treated at 42 degrees and 44 degrees C. Both the biosynthetic parameters were shown to recover after a mild heat shock at 42 degrees C with approximately the same kinetics. The treatment at 44 degrees C led to a full inhibition of Ig production, and the protein electrophoretic pattern was not recovered to normal state within 4-6 hours. The synthesis of heat shock proteins (HSP) was found only after the treatment at 42 degrees C. It is suggested that the expression of HSP is necessary for the recovery of hybridoma cell activities.
Smagina
L V
LV
Margulis
B A
BA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D004589:Electrophoresis, Disc; D006360:Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis; D006358:Hot Temperature; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D007370:Interferon Type I / Q000276:immunology; D051379:Mice; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D011863:Radioimmunoassay; D013696:Temperature; D013997:Time Factors
D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins; D007370:Interferon Type I; D011506:Proteins
Belkovyĭ sintez i produktsiia antitel v kletkakh gibridomy pri gipertermii.