Riboflavin-binding protein from reptiles: a comparison with avian riboflavin-binding proteins.
1. Riboflavin-binding protein (RBP) has been isolated for the first time from reptilian sources. 2. RBP from eggs of Python molurus (Indian python) and Chrysemys picta (painted turtle) has been isolated and compared to RBP from Gallus gallus domesticus (chicken), a well-characterized protein, and a newly isolated RBP from Cairina moschata (Muscovy duck). 3. Each of the proteins is phosphorylated and glycosylated. 4. The ratio of riboflavin binding to protein is 1:1 and the KD for each protein is between 1-3 nM. 5. The mol. wts, different for each species, range from 30,000-40,000, with the reptilian proteins being approx. 10,000 larger than the avian proteins.
Abrams
V A
VA
Department of Chemistry, University of Delaware, Newark 19716.
McGahan
T J
TJ
Rohrer
J S
JS
Bero
A S
AS
White
H B
HB
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D004372:Ducks / Q000378:metabolism*; D004527:Egg Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification; D026901:Membrane Transport Proteins; D008970:Molecular Weight; D010750:Phosphoproteins / Q000302:isolation & purification; D012911:Snakes / Q000378:metabolism*; D013045:Species Specificity; D014426:Turtles / Q000378:metabolism*
D000596:Amino Acids; D002352:Carrier Proteins; D004527:Egg Proteins; D006023:Glycoproteins; D026901:Membrane Transport Proteins; D010750:Phosphoproteins; C019079:riboflavin-binding protein