Demonstration of N-acetylchondrosine-degrading beta-glucuronidase in rabbit liver.
N-Acetylchondrosine was incubated at pH 4.0 with a rabbit-liver crude enzyme extract. Gel filtration of the reaction products on Sephadex G-15 revealed the presence of monosaccharide liberated from the disaccharide. The monosaccharide fraction was analyzed by gas-liquid chromatography, and identified as a mixture of glucuronic acid and N-acetylgalactosamine. These results indicate the presence of beta-glucuronidase, which degrades N-acetylchondrosine, in rabbit liver. The discovery of the presence of this enzyme may help to establish the complete degradation process of chondroitin sulfates.
Nakamura
T
T
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
Takagaki
K
K
Majima
M
M
Endo
M
M
D000818:Animals; D002850:Chromatography, Gel; D004187:Disaccharides / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D020723:Glucuronic Acid; D005966:Glucuronidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D008297:Male; D009005:Monosaccharides / Q000378:metabolism; D011817:Rabbits
D004187:Disaccharides; D005965:Glucuronates; D009005:Monosaccharides; C056533:N-acetylhyalobiuronic acid; C033269:N-acetylchondrosine; D020723:Glucuronic Acid; D005966:Glucuronidase