The abundance of calmodulin mRNAs is regulated in phosphorylase kinase-deficient skeletal muscle.
In the I/Lyn mouse strain a mutation on the X chromosome results in a deficiency of the major calmodulin-regulated enzyme in skeletal muscle, phosphorylase kinase. Calmodulin has been identified as the delta-subunit of phosphorylase kinase, and it is estimated that approximately 40% of the total calmodulin in rabbit skeletal muscle is associated with the phosphorylase kinase hexadecamer (alpha, beta, gamma, delta)4. The absence of phosphorylase kinase in I/Lyn skeletal muscle results in a reduction in the total amount of calmodulin. The mechanisms affecting this reduction were investigated by comparing the abundance and heterogeneities in calmodulin mRNAs between normal and phosphorylase kinase-deficient skeletal muscles. The results demonstrate that in normal tissue there are four species of calmodulin mRNA distinguished by their molecular weight. All four of these species are present in the deficient tissue, and none of them are preferentially reduced. However, there is a 54% reduction in all four mRNAs as well as in calmodulin in the deficient skeletal muscle relative to normal skeletal muscle. These results indicate that the expression of calmodulin mRNAs is coordinated with the expression of its major enzyme target in skeletal muscle.
Bender
P K
PK
Department of Biology, University of Virginia, Charlottesville 22901.
Dedman
J R
JR
Emerson
C P
CP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002147:Calmodulin / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010764:Phosphorylase Kinase / Q000172:deficiency* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*
D002147:Calmodulin; D046911:Macromolecular Substances; D012333:RNA, Messenger; D004247:DNA; D010764:Phosphorylase Kinase