Molecular organization of the DQ subregion (DO-DX-DV-DQ) of the human MHC and its evolutionary implications.
An overlapping set of cosmid clones from the homozygous DR7 B lymphoblastoid cell line MANN linking the HLA-DO through -DQ subregions is described. This region encompasses 280 kb of DNA, including DQ alpha, DQ beta, DX alpha, DX beta, DO beta, and recently indentified L chain sequences termed DV beta. The orientation and grouping of the alpha- and beta-chains is comparable with an analogous murine class II subregion and also with the HLA-DR alpha and -DR beta chains, suggesting that the arrangement of the constituent genes of class II subregions predates the mouse/human divergence.
Blanck
G
G
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Strominger
J L
JL
D005075:Biological Evolution; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D003360:Cosmids; D005796:Genes; D005807:Genes, Overlapping; D008040:Genetic Linkage; D006681:HLA-D Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans
D006681:HLA-D Antigens; D006683:HLA-DQ Antigens