[Micropreparative thin-layer peptide maps in the molecular diagnosis of abnormal human hemoglobins].
General factors determining the possibility of application of peptide maps in thin layer of microcrystalline cellulose as a micropreparative method in molecular diagnostics of abnormal hemoglobins were studied. The effects of absorbtional capacity of cellulose and amino acid impurities in it, choice of eluent and elution technique, peptide structure and extent of its modification in staining as well as completeness and specificity of globin chain enzymatic digestion on peptides extraction from thin layer were analysed. The results of structural identification of Hb D Punjab beta 121 Glu----Gln at a Cypriot; Hb O Arab; beta 121 Glu----Lys at a Bulgarian woman, living in Kalinin region (the second case in the USSR); Hb M Saskatoon beta 63 His----Tyr at a woman from Georgia (the second case in the USSR); Hb Buenos Aires beta 85 Phe----Ser at a Russian girl (the first case in the USSR and the third case in the world); Hb Dagestan alpha 60 Lys----Glu at two members of a Lesgin family from Dagestan; Hb Agenogi beta 90 Glu----Lys at a Hungarian woman; Hb Setif alpha 94 Asp----Tyr at three patients from Cyprus and Hb Detroit beta 95 Lys----Asn at an Azerbaijanian woman (the first case in the USSR and the second case in the world) are presented.
Spivak
V A
VA
Aseeva
E A
EA
Lutsenko
I N
IN
D000596:Amino Acids / Q000032:analysis; D006453:Hemoglobinopathies / Q000175:diagnosis; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping / Q000379:methods*
D000596:Amino Acids; D006455:Hemoglobins, Abnormal; D010446:Peptide Fragments
Mikropreparativnye tonkosloĭnye peptidnye karty v molekuliarnoĭ diagnostike anomal'nykh gemoglobinov cheloveka.