Pertussis toxin stimulates delayed-onset, Ca2+-dependent catecholamine release and the ADP-ribosylation of a 40 kDa protein in bovine adrenal chromaffin cells.
Pertussis toxin was found to stimulate catecholamine release from bovine adrenal chromaffin cells in a Ca2+-dependent manner and in the absence of any stimulatory or inhibitory agonists for this cell. The release of catecholamine was associated with the ADP-ribosylation of an approx. 40 kDa protein present in the total membrane fraction. These results are consistent with the existence of an exocytosis-linked G-protein.
Brocklehurst
K W
KW
Laboratory of Cell Biology and Genetics, National Institute of Diabetes, and Digestive and Kidney Diseases, Bethesda, MD 20892.
Pollard
H B
HB
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D007700:Kinetics; D037342:Pertussis Toxin; D011506:Proteins / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D002395:Catecholamines; D011506:Proteins; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D037342:Pertussis Toxin; D002118:Calcium