ATP-content and kinetics of acrosome reaction in human spermatozoa: influence of various culture media and incubation time.
The ATP content and the kinetics of acrosome reaction of isolated human spermatozoa were investigated during an in vitro culture period of 3 hours in 3 serum-enriched media commonly adopted in IVF and GIFT procedures (Earle solution, Ham F10 and Menezo B2). The ATP concentration in spermatozoa did not change between 1 and 3 hr of incubation and no differences were seen using the three media under investigation. The percentage of acrosome reactions significantly increased during culture, to a similar extent in the three media. The results suggest that each of the three media is equally well suitable for human sperm culture in preparation of IVF and GIFT procedures.
Dolci
S
S
Dipartimento di Sanità Pubblica e Biologia Cellulare, II Università di Roma Tor Vergata, Italy.
Manna
C
C
Jannini
E A
EA
Piccione
E
E
Pasetto
N
N
D000177:Acrosome; D000255:Adenosine Triphosphate / Q000032:analysis*; D003470:Culture Media; D006801:Humans; D007700:Kinetics; D008297:Male; D013094:Spermatozoa / Q000032:analysis*; D013997:Time Factors
D003470:Culture Media; D000255:Adenosine Triphosphate