Cellular effects of T-2 mycotoxin on two different cell lines.
The effects of T-2 toxin on protein synthesis, respiratory chain activity of the mitochondria, cell lysis and toxin-cell binding were compared in the toxin-sensitive bovine kidney cell line (MDBK) and in the toxin-resistant Chinese hamster ovary cell line (CHO). Protein synthesis and mitochondrial activity were 10-fold less sensitive in CHO cells as compared to MDBK (50% inhibition = 10-15 ng/ml vs. 1-1.5 ng/ml, respectively). Lytic activity, as determined by release of 51Cr from cells incubated at 4 degrees C, was not detected at 5 h or 24 h in either of the cell lines. However, at 24 h, CHO cells released less 51Cr than non-toxin-exposed controls, indicating that some membrane interaction does occur. Both cell lines equally bound [3H]T-2 toxin at 4 degrees C. At 37 degrees C, the MDBK cells take up twice as much [3H]T-2 toxin at 2 h and 6 h. These results indicate that T-2 toxin mediates a number of effects on the cell at the level of the membrane, protein synthesis and probably mitochondrial activity toward 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction.
Holt
P S
PS
Veterinary Toxicology Research Unit, United States Department of Agriculture, College Station, TX.
DeLoach
J R
JR
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D002460:Cell Line; D002477:Cells / Q000187:drug effects*; D002860:Chromium Radioisotopes / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005057:Eukaryotic Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010053:Ovary; D011500:Protein Synthesis Inhibitors / Q000633:toxicity; D012717:Sesquiterpenes / Q000633:toxicity*; D013605:T-2 Toxin / Q000378:metabolism / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles
D002860:Chromium Radioisotopes; D011500:Protein Synthesis Inhibitors; D012717:Sesquiterpenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D013605:T-2 Toxin