[Specific determination of free urinary cortisol using high performance liquid chromatography].
Specific assay of free urinary cortisol is difficult. Only high pressure liquid chromatography (HPLC) is suitable for this problem but requires pretreatment of urines. Seven protocols of purification are compared and a routine method is proposed. Performance of this is evaluated and discussed in patients with corticoid therapy.
Patricot
M C
MC
Laboratoire d'Hormonologie, Centre hospitalier Lyon-Sud, Pierre-Bénite.
Fraass
H
H
Mathian
B
B
Revol
A
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*
D006854:Hydrocortisone
Dosage spécifique du cortisol libre urinaire par chromatographie liquide de haute performance.