[Fluorescence characteristics of dynamic changes in the DNA structure of peripheral blood cells in irradiated rats].
A simple approach is proposed to determine locally denatured sites and stability characteristics of secondary DNA structure. The method is based on the analysis of the initial part of melting curve and the determination of changes in the optical density of DNA after heating up to a fixed temperature. The potentiality of the approach is illustrated by the experiments with DNA containing defects in the secondary structure caused by gamma-irradiation in vitro. The sensitivity of the method is less than 0.2 Gy.
Ivanov
S D
SD
Remizova
I V
IV
Reshchikov
A M
AM
Kovan'ko
E G
EG
Komar
V E
VE
D000818:Animals; D001482:Base Composition / Q000528:radiation effects; D002467:Cell Nucleus / Q000032:analysis / Q000528:radiation effects; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004996:Ethidium; D005456:Fluorescent Dyes; D005720:Gamma Rays; D007211:Indoles; D007962:Leukocytes / Q000032:analysis / Q000528:radiation effects*; D008297:Male; D051381:Rats; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA; D004996:Ethidium
Fluorestsentnaia kharakteristika dinamiki izmeneniia struktury DNK kletok perifericheskoĭ krovi obluchennykh krys.