Reliable diagnosis of Trichosomoides crassicauda in the urinary bladder of the rat.
Two reliable methods are described for identifying infection of laboratory rats with the nematode Trichosomoides crassicauda. The first is a rapid method where cryostat sections of the rat urinary bladder are stained with acridine orange and viewed under a fluorescence microscope. The second involves the stabilization of the bladder surface prior to examination using scanning electron microscopy (SEM).
Cornish
J
J
Department of Medicine, University of Auckland, New Zealand.
Vanderwee
M A
MA
Findon
G
G
Miller
T E
TE
D000818:Animals; D005260:Female; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009348:Nematoda / Q000302:isolation & purification* / Q000648:ultrastructure; D009349:Nematode Infections / Q000175:diagnosis / Q000662:veterinary*; D051381:Rats / Q000469:parasitology*; D012376:Rodent Diseases / Q000175:diagnosis*; D001743:Urinary Bladder / Q000469:parasitology / Q000648:ultrastructure; D001745:Urinary Bladder Diseases / Q000175:diagnosis / Q000662:veterinary*; D014556:Urine / Q000469:parasitology