Isolation and identification of coenzyme Q from Leishmania donovani.
Crystalline coenzyme Q (CoQ) was obtained from Leishmania donovani promastigotes by extraction of the unsaponifiable material with hexane, chromatography on a Florisil column and crystallization from ethanol. Crystalline CoQ in ethanol revealed an ultraviolet-peak, at 275 nm, which disappeared upon reduction with NaBH4. Polarographic and mass fragmentographic measurements were characteristic of CoQ homologs. Reversed-phase thin-layer chromatography revealed that it is CoQ9.
Rassam
M B
MB
Department of Chemistry, College of Sciences, University of Baghdad, Jadyria, Iraq.
Shanshal
M
M
Gargees
G S
GS
D000818:Animals; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D007893:Leishmania donovani / Q000032:analysis*; D011048:Polarography; D013056:Spectrophotometry, Ultraviolet; D014451:Ubiquinone / Q000032:analysis / Q000302:isolation & purification*
D014451:Ubiquinone