Microfluorometry of pectic materials in the dehiscence zone of almond (Prunus dulcis [Mill.] DA Webb) fruits.
We examined the middle lamella and the primary and secondary walls in almond pericarp dehiscence zone cells using a fluorescent cytochemical method which permitted specific, quantitative detection of pectic cell wall materials. Glycol methacrylate-embedded sections were stained with coriphosphine and pectin-specific fluorescent emissions at 630 nm were quantified using green excitation (546 nm). Examination of sectioned material extracted with purified pecto-lytic enzyme preparations was used to demonstrate the relative specificity of the staining reaction for pectic substances.
Weis
K G
KG
Department of Pomology, University of California, Davis 95616.
Polito
V S
VS
Labavitch
J M
JM
D000609:Aminoacridines; D002473:Cell Wall / Q000032:analysis; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D008856:Microscopy, Fluorescence; D010368:Pectins / Q000032:analysis*; D010944:Plants / Q000032:analysis*
D000609:Aminoacridines; D005456:Fluorescent Dyes; C018024:coriphosphine; D010368:Pectins