Fucose-containing oligosaccharides from human milk from a donor of blood group 0 Le(a) nonsecretor.
The neutral oligosaccharides from the milk of a single donor with blood group 0, Lewis(a+b-) nonsecretor were separated into 18 fractions essentially according to the number of carbohydrate constituents using gel permeation chromatography on Biogel P-4 and Fractogel TSK HW-40. Further separation was achieved by HPLC and by HPTLC after reduction and peracetylation. The fractions obtained were analysed by FAB mass spectrometry with and without derivatization and by one- and two-dimensional proton NMR. Besides the already described 3-fucosidolactose, fucopentaose II (2), fucopentaose III (6) and difucohexaose II (4) the following fucosylated oligosaccharides could be identified. Among the higher oligosaccharides a branched lacto-N-decaose (12) was obtained in pure form after removal of the fucose residues by mild acid treatment.
Bruntz
R
R
Institut für Physiologische Chemie der Universität Bonn.
Dabrowski
U
U
Dabrowski
J
J
Ebersold
A
A
Peter-Katalinić
J
J
Egge
H
H
D000017:ABO Blood-Group System; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005643:Fucose / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D007983:Lewis Blood Group Antigens; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008895:Milk, Human / Q000032:analysis*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*
D000017:ABO Blood-Group System; D007202:Indicators and Reagents; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D005643:Fucose