title: The attachment of UDP-hexosamines to the ribosomes isolated from rat liver.
abstract: The binding of UDP-N-acetylhexosamines with purified ribosomes was studied and it was found that the radioactive nucleotides can be attached to these particles. The radioactivity of the purified ribosomal pellet depends on the amounts of ribosomes and UDP-N-acetylhexosamines. Some characteristics of the binding system indicate that the attachment of UDP-sugar to ribosome does not require the participation of glycosyltransferases. The results of the competition experiment would suggest that there are specific sites on ribosomes for the binding of UDP-N-acetylglucosamine.
authors.affiliation: Department of General and Organic Chemistry, Institute of Chemistry, Medical School, Białystok, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Kopacz-Jodczyk
authors.forename: A
authors.initials: A
authors.lastname: Paszkiewicz-Gadek
authors.forename: W
authors.initials: W
authors.lastname: Gałasiński
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000378:metabolism*; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D009702:Nucleoside Diphosphate Sugars; D014539:Uridine Diphosphate Sugars; D014537:Uridine Diphosphate N-Acetylglucosamine; D014536:Uridine Diphosphate N-Acetylgalactosamine; D000117:Acetylglucosamine