Transmission of the Ca2+-regulatory signal in skeletal muscle thin filaments.
Studies dealing with some aspects of the thin-filament based regulatory mechanism of striated muscle are discussed. A key event is a Ca2+-induced shift of TnI from a binding site on actin to TnC, as indicated by optical and crosslinking experiments. The regions concerned involve residues 1-12 in actin, residues 96-116 in TnI, and residues 89-100 in TnC. These changes in interaction are accompanied by changes in TnC and TnI that occur on a millisecond time scale which is consistent with their being part of the regulatory processes underlying regulation in vivo.
Gergely
J
J
Department of Muscle Research, Boston Biomedical Research Institute, Massachusetts.
Grabarek
Z
Z
Leavis
P C
PC
Strasburg
G
G
Tao
T
T
Wang
C L
CL
D008841:Actin Cytoskeleton / Q000502:physiology*; D000199:Actins / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology*; D004735:Energy Transfer; D007700:Kinetics; D009132:Muscles / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D014336:Troponin / Q000378:metabolism
D000199:Actins; D014336:Troponin; D002118:Calcium