title: The effect of alpha-lactalbumin on the thermotropic phase behaviour of phosphatidylcholine bilayers, studied by fluorescence polarization, differential scanning calorimetry and Raman spectroscopy.
abstract: The effects of bovine alpha-lactalbumin on the thermotropic properties of dimyristoylphosphatidylcholine liposomes are studied by Raman spectroscopy, fluorescence polarization and differential scanning calorimetry. The Raman spectrum reveals the drastic effects of the protein on the phospholipid structure. The transition temperature shifts downwards and the inter- and intrachain order in the lipid matrix progressively diminish with increasing protein concentration. Up to a lipid to protein molar ratio R = 25, the bilayer structure however is maintained. From fluorescence polarization data we conclude that the protein restricts the mobility of the DPH probe. In view of the Raman results, the lower probe mobility obviously cannot be associated with a more rigid lipid matrix. Nevertheless the transition temperatures of the alpha-lactalbumin-phospholipid complex increases. DSC measurements give no decisive way out for this discrepancy. These results confirm that different types of lipid order are involved in lipid-protein interactions. Compared to the free protein, the alpha-helicity of the protein has increased in the complex.
authors.affiliation: Interdisciplinair Research Centrum, K.U. Leuven Campus Kortrijk, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Van Dael
authors.forename: F
authors.initials: F
authors.lastname: Van Cauwelaert
mesh_terms: D002152:Calorimetry, Differential Scanning; D004134:Dimyristoylphosphatidylcholine / Q000378:metabolism*; D006358:Hot Temperature; D007768:Lactalbumin / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D007768:Lactalbumin; D004134:Dimyristoylphosphatidylcholine