Detectability and measurability of amoscanate in plasma by TLC and HPLC.
Although amoscanate has been established as a potent antischistosomal drug, its detectability and measurability in biological fluids has been in doubt. Experiments have been carried out to show that these measurements can be made. High pressure liquid chromatographic (HPLC) and thin layer chromatographic (TLC) analyses have been performed on plasma samples incubated with plasma at 37 degrees C. Specific chemical detection methods of functional groups in the molecule were used to establish the identity of the drug on TLC plates, and the results were found to correlate with the HPLC analyses.
Kofi-Tsekpo
W M
WM
Kenya Medical Research Institute, Traditional Medicines and Drugs Research Centre, Nairobi.
Karekezi
C W
CW
D000814:Aniline Compounds / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000097:blood*; D006801:Humans; D017879:Isothiocyanates; D013861:Thiocyanates / Q000097:blood*
D000814:Aniline Compounds; D017879:Isothiocyanates; D013861:Thiocyanates; D004159:Diphenylamine; C012391:amoscanate