Interaction of abnormal antithrombin-III Toyama with cultured porcine aortic endothelial cells.
The abnormal Antithrombin-III (AT-III) Toyama has no ability to interact with heparin in the mast cells or heparin-like glycosaminoglycans on the surface of vascular endothelial cells, so that it has low heparin cofactor activity. In the present study, we have measured the binding capacity of 125I-labeled AT-III to cultured porcine aortic endothelial cells and estimated the inactivation of thrombin by AT-III in the presence of endothelial cells. The abnormal AT-III Toyama had low binding capacity to endothelial cells and had lower heparin cofactor antithrombin activity in the presence of endothelial cells than that of normal AT-III. Our results suggested that heparin-like glycosaminoglycans on endothelial cells play as important a role as heparin does in the regulation of antithrombin activity.
Saito
S
S
Third Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Takahashi
K
K
Sakuragawa
N
N
D000818:Animals; D000990:Antithrombin III / Q000378:metabolism*; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D001667:Binding, Competitive; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D013552:Swine
C039475:antithrombin III Toyama; D000990:Antithrombin III