title: Retrospective DNa analysis of T3/T4 breast carcinoma using cytophotometry and flow cytometry. A comparative study with prognostic evaluation.
abstract: Tumor cells from 72 patients with advanced breast carcinoma (T3/T4) were analyzed for their DNA content by cytophotometry and flow cytometry (FCM). Both methods were able to subdivide the tumors into groups with different prognoses. Patients with a normal/near-normal DNa content in the tumor had a better prognosis than did those with aneuploid tumors. FCM measurements of DNA content gave a better discrimination for both survival (P = .019) and disease-free survival (P = .059) than did cytophotometry (P = .105 and P = .067, respectively). These results indicate that FCM analysis of paraffin-embedded material is a more accurate method than is cytophotometry for characterizing the DNA content of breast carcinoma cells. The method could be useful in both retrospective studies and in daily diagnostic work.
authors.affiliation: Department of Pathology, University of Umeå, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Roos
authors.forename: C
authors.initials: C
authors.lastname: Arnerlöv
authors.forename: S
authors.initials: S
authors.lastname: Emdin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000032:analysis / Q000401:mortality / Q000473:pathology*; D003592:Cytophotometry / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D004247:DNA