Production of a specific antibody against pyruvate kinase type M2 using a synthetic peptide.
The pyruvate kinase isozymes M1 and M2 are structurally and immunologically closely related. To obtain an antibody which discriminates between these two forms, a synthetic tetradecapeptide with a sequence specific for pyruvate kinase type M2 from rats was constructed. Antisera from rabbits, immunized with this peptide, reacted specifically with the M2-type holoenzyme of both rat and human origin, and did not cross-react with the M1-type isozyme. This was established by immunoblot analysis, both under dissociating and non-dissociating conditions.
Oude Weernink
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PA
Department of Haematology, University Hospital Utrecht, The Netherlands.
Rijksen
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Staal
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D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D007527:Isoenzymes / Q000276:immunology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology; D011770:Pyruvate Kinase / Q000276:immunology*; D051381:Rats
D000906:Antibodies; D007527:Isoenzymes; D009842:Oligopeptides; D011770:Pyruvate Kinase