Comparison of four methods for the determination of sulphonamide concentrations in human plasma.
Analysis of sulphadimethoxine in plasma from patients treated with this drug was performed with four chemical methods: Rieder's modification of the Bratton-Marshall technique and author's modification of the Morris technique, which are supposed to measure "potentially active fraction of sulphonamides", i.e., mainly unchanged sulphonamide; the Bratton-Marshall method, which enables the determination of "free sulphonamide", i.e., the sum of unchanged sulphonamide and glucuronide conjugate; and high performance liquid chromatography which made possible the determination of unchanged sulphadimethoxine. The spectrophotometric methods based upon Bratton-Marshall reaction gave very few higher results as compared with liquid chromatography. The differences between spectrophotometric and chromatographic methods were small; therefore, all four methods can be used for determination of sulphonamide concentrations in plasma during monitored treatment with these drugs.
Klimowicz
A
A
Pomeranian Medical Academy Clinic of Dermatology, Szczecin, Poland.
D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013053:Spectrophotometry; D013412:Sulfadimethoxine / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use
D013412:Sulfadimethoxine