Prostaglandin and thromboxane synthesis by tissue slices from human aortic aneurysms.
Sliced portions of the walls of human aortic aneurysms were incubated with extracts of human plasma and serum to determine the profile of prostanoid production. 6-Oxo-prostaglandin (PG) F1 alpha, PGE2, PGF2 alpha, and thromboxane (TX) B2 were measured by gas chromatography/electron capture mass spectrometry. 6-Oxo-PGF1 alpha, the stable hydrolysis product of PGI2, was the major cyclooxygenase product but substantial amounts of TXB2 (the hydrolysis product of TXA2), with smaller amounts of PGE2 and PGF2 alpha were also synthesised. These prostanoids could contribute to the response of the vascular wall to injury, thereby influencing the disease process. Serum extracts stimulated PGI2 and TXA2 synthesis, probably as a result of their Ca2+ content.
Ritter
J M
JM
Department of Clinical Pharmacology, Royal Postgraduate Medical School, London, U.K.
Frazer
C E
CE
Powell
J T
JT
Taylor
G W
GW
D000328:Adult; D000368:Aged; D001014:Aortic Aneurysm / Q000378:metabolism*; D002118:Calcium / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D011453:Prostaglandins / Q000096:biosynthesis*; D013931:Thromboxanes / Q000096:biosynthesis*
D011453:Prostaglandins; D013931:Thromboxanes; D002118:Calcium