Full term development of mouse eggs fertilized by a spermatozoon microinjected under the zona pellucida.
A mature motile mouse spermatozoon was microinjected under the zona pellucida of mouse eggs. Twenty-five percent of eggs were fertilized, and 54% of these developed to normal fetuses or to term after transfer to pseudopregnant recipients. These results provide a quantitative estimate of the minimum proportion of spermatozoa in a population that are able to contribute to normal development--at least 54% of mature individuals that were able to fertilize the egg after microinjection, or at least 13 1/2% (25% of 54%) of the total population of mature sperm. The production of normal young shows that sperm microinjection is a feasible means for the treatment of severe male infertility in the human and in other species.
Mann
J R
JR
Centre for Early Human Development, Monash University, Clayton, Victoria, Australia.
D000818:Animals; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D051379:Mice; D008845:Microinjections; D010063:Ovum / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*; D015053:Zygote / Q000502:physiology*