title: Identification of the C5a des Arg cochemotaxin. Homology with vitamin D-binding protein (group-specific component globulin).
abstract: The chemotactic activity of human C5a des Arg is enhanced significantly by an anionic polypeptide (cochemotaxin) in normal human serum and plasma. The cochemotaxin attaches to sialic acid residues within the oligosaccharide chain of native C5a des Arg to form a complex with potent chemotactic activity for human PMN. We investigated the nature of the cochemotaxin and found that vitamin D-binding protein is the putative cochemotaxin. Vitamin D-binding protein enhanced the chemotactic activity of native C5a des Arg, but had no effect on the chemotactic activity of either native C5a or FMLP. Sialic acid prevented both enhancement by vitamin D-binding protein of the chemotactic activity of native C5a des Arg and formation of C5a des Arg-vitamin D-binding protein complexes, detected by molecular sieve chromatography. Furthermore, vitamin D-binding protein and cochemotaxin exhibited identical molecular weights, isoelectric points, antigenic reactivity, and amino acid composition.
authors.affiliation: Rosalind Russell Arthritis Research Laboratory, University of California, San Francisco 94143.
authors.forename: H D
authors.initials: HD
authors.lastname: Perez
authors.forename: E
authors.initials: E
authors.lastname: Kelly
authors.forename: D
authors.initials: D
authors.lastname: Chenoweth
authors.forename: F
authors.initials: F
authors.lastname: Elfman
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000906:Antibodies / Q000502:physiology; D002630:Chemotactic Factors / Q000097:blood*; D002634:Chemotaxis, Leukocyte; D003182:Complement C5 / Q000031:analogs & derivatives* / Q000302:isolation & purification; D015937:Complement C5a, des-Arginine; D004347:Drug Interactions; D006801:Humans; D014809:Vitamin D-Binding Protein / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D015054:Zymosan
chemical_list: D000596:Amino Acids; D000906:Antibodies; D002630:Chemotactic Factors; D003182:Complement C5; D015937:Complement C5a, des-Arginine; D014809:Vitamin D-Binding Protein; D015054:Zymosan