Olive (Olea europea) pollen allergens--I. Immunochemical characterization by immunoblotting, CRIE and immunodetection by a monoclonal antibody.
The pattern of reactivity of the Olea europea crude extract antigens was analysed after electroblotting to nitrocellulose from SDS-PAGE. The antigens contained in the 17, 19 and 42 K bands were most reactive with specific IgE from individual sera. Following immunization with a crude extract, one monoclonal antibody (OL-1) was raised against components which exhibited IgE binding capacity in electroblotting and crossed radioimmunoelectrophoresis (CRIE). Monoclonal antibody OL-1 reacted with the 17 and 19 K antigens and with three arcs of crossed immunoelectrophoresis (CIE), one of which is considered to contain a major allergen by CRIE.
Lauzurica
P
P
Department of Immunology, Fundación Jimenéz Díaz Avda, Madrid, Spain.
Gurbindo
C
C
Maruri
N
N
Galocha
B
B
Diaz
R
R
Gonzalez
J
J
García
R
R
Lahoz
C
C
D000485:Allergens / Q000032:analysis*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007073:Immunoglobulin E / Q000276:immunology*; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
D000485:Allergens; D000911:Antibodies, Monoclonal; D010455:Peptides; D007073:Immunoglobulin E