title: Combined treatment of colon adenocarcinoma in rats with tumor necrosis factor and the interferon inducer ABPP.
abstract: It is well documented that the antitumor capacity of tumor necrosis factor (TNF) can be enhanced by interferons (IFNs), notably IFN-gamma. The aim of this study was to investigate the efficacy of a combined treatment with TNF and the IFN-inducer 2-amino-5-bromo-6-phenyl-4-pyrimidinone (ABPP) on a transplantable colon carcinoma (CC531) in rats. The tumor was implanted under the kidney capsule of syngeneic rats; the tumors were removed a week after implantation and growth was assessed by weighing. The animals were treated with 1 microgram of TNF, given i.v. on days 0, 2, and 4; and with 250 mg/kg of ABPP, administered i.p. on days 0 and 1. The results of two separate experiments indicated that both TNF and ABPP had a significant inhibitory effect on tumor growth. Combined, the two agents were found to act additively. In the dosage used, TNF toxicity was mild, transient, and not influenced by ABPP.
authors.affiliation: Department of Surgery, Erasmus University Rotterdam, The Netherlands.
authors.forename: R L
authors.initials: RL
authors.lastname: Marquet
authors.forename: A M
authors.initials: AM
authors.lastname: Eggermont
authors.forename: R W
authors.initials: RW
authors.lastname: de Bruin
authors.forename: W
authors.initials: W
authors.lastname: Fiers
authors.forename: J
authors.initials: J
authors.lastname: Jeekel
mesh_terms: D000230:Adenocarcinoma / Q000628:therapy*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D003110:Colonic Neoplasms / Q000628:therapy*; D003596:Cytosine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004359:Drug Therapy, Combination; D007369:Interferon Inducers / Q000008:administration & dosage* / Q000627:therapeutic use; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage* / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D007369:Interferon Inducers; D014409:Tumor Necrosis Factor-alpha; D003596:Cytosine; C033560:bropirimine