On the determination of species fractions from ligand-binding data. Application to human hemoglobin.
A method outlined in a previous study (S.J. Gill, H.T. Gaud, J. Wyman and G. Barisas, Biophys. Chem. 8 (1978) 53) is applied for the determination of species fractions from ligand-binding data for the oxygen reaction with human hemoglobin. The results obtained by this alternative approach, which is based on the solution of a system of linear equations, are consistent with those obtained using nonlinear least-squares analysis.
Di Cera
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Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.
Gill
S J
SJ
D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008433:Mathematics; D008962:Models, Theoretical; D010108:Oxyhemoglobins / Q000378:metabolism; D011485:Protein Binding
D006454:Hemoglobins; D008024:Ligands; D010108:Oxyhemoglobins