Evaluation of in vitro methods for testing susceptibility of anaerobes to ampicillin-sulbactam and amoxicillin-clavulanic acid.
A total of 97 anaerobic bacteria were tested for susceptibility to ampicillin, ampicillin-sulbactam, and amoxicillin-clavulanic acid by broth microdilution and disk elution methods, the results of which were compared with those of the reference agar dilution method. With the broth microdilution method, approximately 95% of MICs were within 1 dilution of those of the reference agar method, with a definite (0.6 to 0.7 dilution) trend toward lower MICs. The disk elution test performed satisfactorily, but additional anaerobic isolates resistant to ampicillin-sulbactam and/or amoxicillin-clavulanic acid (currently rare) are needed to assure the predictability of resistance by the disk elution test.
Fuchs
P C
PC
St. Vincent Hospital and Medical Center, Portland, Oregon 97225.
Barry
A L
AL
Jones
R N
RN
D000658:Amoxicillin / Q000494:pharmacology*; D000667:Ampicillin / Q000494:pharmacology*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000494:pharmacology*; D004338:Drug Combinations; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013407:Sulbactam / Q000494:pharmacology*
D002969:Clavulanic Acids; D004338:Drug Combinations; D019818:Clavulanic Acid; D000667:Ampicillin; D000658:Amoxicillin; D013407:Sulbactam