title: Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing.
abstract: We describe an investigation of proteinuria using Pharmacia PhastSystemTM electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.
authors.affiliation: Unit for Cancer Research, University of Leeds, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: Jackson
authors.forename: C J
authors.initials: CJ
authors.lastname: Sampson
authors.forename: E H
authors.initials: EH
authors.lastname: Cooper
authors.forename: D
authors.initials: D
authors.lastname: Heney
authors.forename: J T
authors.initials: JT
authors.lastname: Brocklebank
mesh_terms: D001322:Autoanalysis; D001536:Bence Jones Protein / Q000652:urine; D002648:Child; D003928:Diabetic Nephropathies / Q000652:urine; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008180:Lupus Erythematosus, Systemic / Q000652:urine; D008297:Male; D009135:Muscular Diseases / Q000652:urine; D011507:Proteinuria / Q000652:urine*; D012967:Sodium Dodecyl Sulfate
chemical_list: D012967:Sodium Dodecyl Sulfate; D001536:Bence Jones Protein