[Reduction of nitroblue tetrazolium by thiols in the presence of vitamin K].
Thiol-containing proteins and low molecular sulfhydryl-containing compounds reduced nitroblue tetrazolium (NBT) in presence of vitamin K3(2-methyl-1,4-naphthoquinone). Cysteine and dithiothreitol (but not glutathione) reduced NBT also in absence of the quinone. Reduction of NBT was accelerated in the weakly alkaline medium and occurred both via O2- -dependent and O2- -independent mechanism. In presence of quinones the thiols appear to affect the manifestation of oxidoreductase reactions estimated by means of NBT-test.
Gerasimov
A M
AM
Zakharov
A S
AS
D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors; D013778:Tetrazolium Salts / Q000378:metabolism*; D014812:Vitamin K / Q000494:pharmacology*
D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D013778:Tetrazolium Salts; D014812:Vitamin K; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase
Vosstanovlenie nitrosinego tetrazoliia tiolami v prisutstvii vitamina K.