title: Orosomucoid (ORM) typing by isoelectric focusing: evidence for an additional duplicated ORM1 locus haplotype and close linkage of two ORM loci.
abstract: Human serum orosomucoid (ORM) exhibits a high variability. Several alleles including a duplicated ORM1 gene (ORM1*2.1) have been identified at two functional ORM loci, ORM1 and ORM2. In this study a modified isoelectric focusing, in which glycerin was omitted from gels, was used to differentiate a new variant ORM1 5 from ORM2 3. The ORM1 5 band was always observed together with the ORM1 2 band. The simultaneous expression could be explained in terms of an additional duplicated ORM1 locus haplotype, ORM1*5.2, whose average frequency was .016 in two Japanese populations. The ORM2*6 found at a polymorphic frequency (.023) was demonstrated to be in association with ORM1*2, indicating the close proximity between ORM1 and ORM2 loci.
authors.affiliation: Department of Legal Medicine, Tottori University School of Medicine, Yonago, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Yuasa
authors.forename: K
authors.initials: K
authors.lastname: Umetsu
authors.forename: K
authors.initials: K
authors.lastname: Suenaga
mesh_terms: D000483:Alleles; D005260:Female; D005787:Gene Frequency; D005796:Genes; D008040:Genetic Linkage; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D009961:Orosomucoid / Q000235:genetics* / Q000302:isolation & purification; D010375:Pedigree; D010641:Phenotype; D011110:Polymorphism, Genetic
chemical_list: D009961:Orosomucoid