Formation of acetaldehyde adducts with ethanol-inducible P450IIE1 in vivo.
Hepatic microsomes, obtained from rats pair-fed liquid diets supplemented with either ethanol or an isocaloric amount of carbohydrates (for 4 weeks), were subjected to crossed immunoelectrophoresis. Anti-acetaldehyde adduct-specific immunoglobulin reacted on the protein blots with a single major 52,000 dalton polypeptide. This same protein was recognized by antibodies specific for P450IIE1, an ethanol-inducible P450 isozyme. Furthermore, a single protein, also reactive with anti-P450IIE1 IgG, was isolated from liver microsomes of ethanol-fed rats by immunoaffinity chromatography on Sepharose-conjugated anti-acetaldehyde adduct IgG. These results indicate that P450IIE1 is a target protein for acetaldehyde binding in liver microsomes in vivo.
Behrens
U J
UJ
Alcohol Research and Treatment Center, Bronx Veterans Administration Medical Center, N.Y.
Hoerner
M
M
Lasker
J M
JM
Lieber
C S
CS
D000079:Acetaldehyde / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D000431:Ethanol / Q000494:pharmacology*; D007527:Isoenzymes / Q000096:biosynthesis*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D000079:Acetaldehyde