High performance liquid chromatography of a new 1,4-dihydropyridine: applications to pharmacokinetic study in dogs.
A new nitrendipine derivative [ethyl-2-(1-piperidino)ethyl-1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)- 3,5-pyridine dicarboxylate hydrochloride;1] was assayed in plasma by high-performance liquid chromatography. After deproteinization and extraction, 1 and nitrendipine, as the external standard, were separated by ion-pair chromatography and measured by UV detection (254 nm). The method is rapid and specific: the limit of sensitivity of the assay was 5 ng/mL and the concentration range was linear between 5 and 2000 ng/mL. In vitro studies showed that, in contrast to nifedipine and nicardipine, 1 and nitrendipine were stable when exposed to light for at least 4 h. Pharmacokinetic parameters obtained in three beagle dogs after oral and intravenous administration are reported. Comparison with a nicardipine pharmacokinetic study showed similar results for the distribution and elimination characteristics of these two drugs.
Greiner
P O
PO
Department of Pharmacokinetics, SIR International, Montrouge, France.
Angignard
D
D
Cahn
J
J
D000284:Administration, Oral; D000818:Animals; D002121:Calcium Channel Blockers / Q000032:analysis / Q000493:pharmacokinetics* / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D008027:Light; D008297:Male; D009529:Nicardipine / Q000493:pharmacokinetics; D009543:Nifedipine / Q000493:pharmacokinetics; D009568:Nitrendipine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000528:radiation effects
D002121:Calcium Channel Blockers; D007202:Indicators and Reagents; C056837:diperdipine; D009568:Nitrendipine; D009529:Nicardipine; D009543:Nifedipine