Determination of residual cephalexin in chick tissues by Bacillus stearothermophilus paper disc assay.
A paper disc method is described for determination of residual cephalexin (CEX) in chick tissues. A trichloroacetic acid extract of plasma and tissues is chromatographed on a macroreticular resin (Diaion HP-20) column to remove endogenous antibacterial substances interfering with the assay. The eluate is evaporated to dryness and the residue, dissolved in methanol-water (1 + 2), is subjected to a paper disc assay using Bacillus stearothermophilus var. calido-lactis C953 NIZO as a test organism. The detection limit was 0.0375 ppm in tissue; the average recovery of CEX ranged from 72.4% in skin to 90.4% in plasma. Water containing 200 or 500 mg/L of CEX was given ad libitum to 2-week-old chicks for 10 days; the highest levels of CEX were found in the kidney, and the lowest were found in muscle at 0 h of withdrawal. CEX disappeared from most tissues at 24 h after withdrawal except from skin of chicks given 500 mg/L. However, the drug was not detected in the skin at 48 h after withdrawal.
Okada
J
J
Takeda Chemical Industries, Ltd, Research Laboratories, Animal Health Products Division, Osaka, Japan.
Higuchi
I
I
Kondo
S
S
Saito
B
B
D000273:Adipose Tissue / Q000032:analysis; D000818:Animals; D002506:Cephalexin / Q000032:analysis* / Q000097:blood / Q000494:pharmacology; D002642:Chick Embryo; D001411:Geobacillus stearothermophilus / Q000187:drug effects*; D007202:Indicators and Reagents; D007668:Kidney / Q000032:analysis; D009132:Muscles / Q000032:analysis; D012867:Skin / Q000032:analysis
D007202:Indicators and Reagents; D002506:Cephalexin