Characterization of Novikoff hepatoma small RNAs homologous to repetitive DNAs.
Three minor small RNA species from Novikoff hepatoma cells, with homology to repetitive DNA sequences, have been identified and characterized. These small RNAs, designated 5.1S, 6S and T3 RNAs, show homology to Alu 1, Alu 2, and Alu 3 sequences, respectively. 6S and T3 RNAs were found both in the nucleus and cytoplasm, whereas 5.1S RNA was not found in the nucleus. Neural tissues were found to contain a 6S-sized BC1 RNA with homology to I.D. sequences; in contrast, the current study shows that Novikoff hepatoma cells contain a 75-80 nucleotide long (T3) RNA, homologous to I.D. sequences. These data suggest that BC1 and T3 small RNAs, homologous to I.D. sequences, are expressed in a tissue-specific manner. These results also show that in addition to the abundant 7SL, 4.5S and 4.5S1 RNAs having homology to repetitive DNA, Novikoff hepatoma cells also contain several minor small RNAs with homology to repetitive sequences.
Reddy
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R
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030.
Henning
D
D
Suh
D
D
D000818:Animals; D004247:DNA; D006367:HeLa Cells; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm; D012342:RNA, Small Nuclear; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D012334:RNA, Neoplasm; D012342:RNA, Small Nuclear; D004247:DNA