Identification of the 80-kDa protein that crosslinks to the cap structure of eukaryotic mRNAs as initiation factor eIF-4B.
Preparations of either crude or purified protein synthesis initiation factors, when tested by crosslinking to the m7G-cap structure of mRNAs, exhibit specific crosslinking to an 80-kDa protein. Polyclonal antibodies specific for eIF-4B precipitate the 80-kDa cap-radiolabeled protein, thereby demonstrating that eIF-4B binds mRNA near its 5'-terminus.
Milburn
S C
SC
Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
Pelletier
J
J
Sonenberg
N
N
Hershey
J W
JW
D000818:Animals; D003432:Cross-Linking Reagents / Q000302:isolation & purification*; D039642:Eukaryotic Initiation Factors; D008970:Molecular Weight; D010448:Peptide Initiation Factors / Q000302:isolation & purification* / Q000378:metabolism; D011233:Precipitin Tests; D012315:RNA Caps / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits
D003432:Cross-Linking Reagents; D039642:Eukaryotic Initiation Factors; D010448:Peptide Initiation Factors; D012315:RNA Caps; D012333:RNA, Messenger; C031799:eIF-4B