Sequence, tissue distribution, and chromosomal localization of mRNA encoding a human glucose transporter-like protein.
cDNA clones encoding a glucose transporter-like protein have been isolated from adult human liver and kidney cDNA libraries by cross-hybridization with the human HepG2/erythrocyte glucose transporter cDNA. Analysis of the sequence of this 524-amino acid glucose transporter-like protein indicates that it has 55.5% identity with the HepG2/erythrocyte glucose transporter as well as a similar structural organization. Studies of the tissue distribution of the mRNA coding for this glucose transporter-like protein in adult human tissues indicate that the highest amounts are present in liver with lower amounts in kidney and small intestine. The amounts of glucose transporter-like mRNA in other tissues, including colon, stomach, cerebrum, skeletal muscle, and adipose tissue, were below the level of sensitivity of our assay. The single-copy gene encoding this glucose transporter-like protein has been localized to the q26.1----q26.3 region of chromosome 3.
Fukumoto
H
H
Howard Hughes Medical Institute, Chicago, IL.
Seino
S
S
Imura
H
H
Seino
Y
Y
Eddy
R L
RL
Fukushima
Y
Y
Byers
M G
MG
Shows
T B
TB
Bell
G I
GI
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007421:Intestine, Small / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D014158:Transcription, Genetic
D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D004247:DNA