A superfusion method for the study of calcium fluxes from pituitary cells.
This paper describes a superfusion technique that allows the study of calcium fluxes from a preparation of anterior pituitary cells. There is easy reproducibility, high sensitivity, controlled conditions that closely resemble those in vivo, possibility to operate sequential treatments on the same sample. These are some of the advantages of this method. An interesting application consists of the possibility of simultaneous measurement of calcium fluxes and hormone release.
Valerio
A
A
Institute of Pharmacology and Experimental Therapeutics, School of Medicine, University of Brescia, Italy.
Memo
M
M
Missale
C
C
Spano
P F
PF
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D009496:Neurotensin / Q000494:pharmacology; D010477:Perfusion; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats
D009496:Neurotensin; D011188:Potassium; D002118:Calcium