title: Requirement for host Fc receptors and IgG antibodies in host immune responses against Rhipicephalus appendiculatus.
abstract: Guinea pigs sensitized by prior feeding of larval Rhipicephalus appendiculatus ticks expressed complete immunity to challenge feeding resulting in 100% tick rejection. Passive transfer of 1 ml of serum from animals expressing resistance into naive animals conferred recipients with significant protection (88% tick rejection). Successful transfer of resistance was blocked by pretreatment of recipients with rabbit IgG but not sheep IgG1. Passive transfer of IgG1 or IgG2 purified from tick-sensitized guinea pig serum by ion-exchange chromatography failed to confer resistance to naive guinea pigs. Furthermore, IgG1 from guinea pigs expressing resistance obtained from serum by passage through a heavy chain specific rabbit anti-guinea pig IgG1 column failed to confer resistance to naive guinea pigs, as did the eluate. These results suggest that both IgG subclasses are needed for the expression of resistance, or IgG1 in conjunction with IgE.
authors.affiliation: Division of Parasitology, National Institute for Medical Research, London, Gt. Britain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Worms
authors.forename: P W
authors.initials: PW
authors.lastname: Askenase
authors.forename: S J
authors.initials: SJ
authors.lastname: Brown
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D005260:Female; D006168:Guinea Pigs; D006790:Host-Parasite Interactions; D007110:Immunity, Active; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology*; D013984:Tick Infestations / Q000276:immunology*; D013987:Ticks / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D011961:Receptors, Fc