Secretion of a lactosaminoglycan-containing glycoprotein by peri-implantation sheep conceptuses.
Sheep conceptuses from day 16 of pregnancy were cultured in the presence of [3H]glucosamine and [14C]leucine and a high-molecular-weight glycoprotein (HMWG) secreted into the culture medium was purified by a combination of anion-exchange and gel filtration chromatography. The HMWG was found to have a molecular weight between 800,000 and 900,000 and to be highly resistant to digestion with pronase. Characteristics of the carbohydrate portion of the purified glycoprotein were examined by selective chemical and enzymatic digestions and lectin binding studies. Mild alkaline reduction was ineffective in disassociating carbohydrate chains from the protein core. Furthermore, the protein was resistant to both O-glycanase and peptide:N-glycanase F. Harsh alkaline reduction caused the release of carbohydrates, however. After pronase digestion of these products, three molecular weight classes of carbohydrates were resolved by Sephadex G-25 chromatography. Two lines of evidence indicate that the HMWG contains lactosaminoglycan components. The intact molecule and two of the molecular weight classes of carbohydrates resolved by harsh alkaline reduction bind Datura stramonium lectin. Binding of HMWG to lectin could be partially inhibited by N-acetyllactosamine and completely inhibited by a mixture of N,N'-diacetylchitobiose and N,N',N"-triacetylchitotriose. Secondly, digestion with endo-beta-galactosidase causes the release of 16% of the [3H]glucosamine from the intact molecule. Therefore, the HMWG of the sheep conceptus is the first reported example of secretion of lactosaminoglycan-containing glycoprotein by peri-implantation embryos.
Newton
G R
GR
Department of Dairy Science, University of Florida, Gainesville 32611.
Hansen
P J
PJ
D000606:Amino Sugars / Q000032:analysis*; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004622:Embryo, Mammalian / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000032:analysis*; D011402:Pronase / Q000378:metabolism; D012756:Sheep
D000606:Amino Sugars; D006023:Glycoproteins; D037102:Lectins; D011134:Polysaccharides; C039448:lactosaminoglycan; D006026:Glycoside Hydrolases; D011402:Pronase