Self-chemical ionization Fourier transform ion cyclotron resonance mass spectrometry: identification and characterization of modified and unmodified bases and nucleosides.
Self-chemical ionization Fourier transform ion cyclotron resonance (FT-ICR) mass spectra are reported for bases, nucleosides, and alkylated and exocyclic adducts of bases and nucleosides. The technique always produces a protonated molecular ion and in the majority of cases this is a single, intense peak. Analysis of a base mixture and a nucleoside mixture demonstrates the technique as an excellent method to identify the constituent compounds qualitatively. The high resolution capabilities and tandem mass spectrometric techniques (msn) in FT-ICR are discussed with respect to developing the technique as a future method to identify and characterize nucleic acid constituents, specifically adducts.
Weller
R R
RR
Department of Industrial Environmental Health Sciences, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261.
Mayernik
J A
JA
Giam
C S
CS
D055598:Chemical Phenomena; D002621:Chemistry; D005583:Fourier Analysis; D013058:Mass Spectrometry; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D010315:Particle Accelerators
D009705:Nucleosides; D009711:Nucleotides