NMR-invisible lactate in blood plasma.
Resonances for lactate are broadened in 500 MHz 1H NMR spectra of human blood plasma and only about one-third is visible in Hahn spin-echo spectra. Similar effects are observed for some other carboxylate anions. Lactate added to the high-Mr fraction of plasma can give rise to peaks which are too broad to observe in either single-pulse or spin-echo spectra. Addition of agents such as NH4Cl of SDS dramatically increases the intensities of lactate peaks. Some glycoproteins appear to broaden lactate resonances.
Bell
J D
JD
Department of Chemistry, Birkbeck College, University of London, England.
Brown
J C
JC
Kubal
G
G
Sadler
P J
PJ
D000643:Ammonium Chloride / Q000494:pharmacology; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D006023:Glycoproteins; D007773:Lactates; D000643:Ammonium Chloride; D019344:Lactic Acid; D012967:Sodium Dodecyl Sulfate