[Isolation of immunoglobulin G-binding factor from blood lymphocytes of cows with lymphocytic leukemia].
Incubation of bovine blood lymphocytes in the medium without serum at 37 degrees C caused the spontaneous release of immunoglobulin G-binding factor (IBF-IgG), which was isolated from the medium by the affinity chromatography on IgG, immobilized on sepharose 4B. The electrophoretic analysis showed one polypeptide chain with a molecular weight of 72000 Dalton. The biological activity of IBF-IgG was tested using the EA-rosette inhibition technique. The antibodies, obtained against IBF-IgG, inhibited both the binding of fluoresceinizotiocianate-labeled IgG to lymphocytes and the EA-rosette formation.
Miliukene
V V
VV
Dikinene
N P
NP
D000818:Animals; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology; D002846:Chromatography, Affinity; D007074:Immunoglobulin G / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology* / Q000662:veterinary; D008222:Lymphokines / Q000097:blood / Q000302:isolation & purification*; D029584:Prostatic Secretory Proteins; D012397:Rosette Formation
D007074:Immunoglobulin G; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors
Poluchenie immunoglobulin G-sviazyvaiushchego faktora iz limfotsitov krovi korov, bol'nykh limfoleĭkozom.