Use of alveolar macrophages in antianoxic drug studies.
Alveolar macrophages are able to adapt their energy metabolism to very difficult survival conditions. Gaseous phase culture is adaptable to alveolar macrophages because it reproduces in vitro conditions very similar to in vivo conditions. It is easy to modify the incubation gas composition for hypoxia and anaerobiosis. Metabolic changes and cell injury were evaluated in three studies carried out after 24 hr of gaseous phase culture in normoxia and in anaerobiosis with a possible treatment with 0.01 microgram/ml vincamine: 1) ATP content assay by bioluminescence, the witness of cell vitality which decreases significantly in anaerobiosis; 2) Lactate assay which shows the metabolism derivation towards the anaerobic pathways; and 3) Tritiated deoxyglucose (DOG) incorporation, which shows glucose requirements after hypoxic incubation, maintaining or recovering a certain level of energetic activity. This incorporation greatly increases after anaerobic culture. Vincamine has no activity in normoxia. The three parameters are not significantly different from control, but in anaerobiosis, vincamine reveals an interesting protective effect. ATP content decreases under treatment and DOG incorporation increases. This demonstrates that vincamine is able to maintain cell metabolic activity for a longer period of time after the beginning of hypoxic trial. Cells can better use their energy storage and the metabolic pathways which enable them to restore themselves, thanks to vincamine treatment. It has been shown that cell membrane integrity was preserved by tests using cytochalasin B. DOG was not incorporated by cells treated with cytochalasin B after 24 hr of anaerobic culture and normally incorporated by control cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Cazin
M
M
Laboratoire de Pharmacologie, Faculté de Pharmacie, Lille, France.
Paluszezak
D
D
Poulain
V
V
Dine
T
T
Bianchi
A
A
Cazin
J C
JC
Aerts
C
C
D000255:Adenosine Triphosphate / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D006168:Guinea Pigs; D000860:Hypoxia / Q000188:drug therapy*; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology*; D013056:Spectrophotometry, Ultraviolet; D014749:Vincamine / Q000494:pharmacology
D007773:Lactates; D000255:Adenosine Triphosphate; D014749:Vincamine; D003847:Deoxyglucose