title: [Temperature quenching of cytochrome P-450 fluorescence in proteoliposomes with different physical states of the lipid bilayer].
abstract: Studies were carried out of temperature quenching of self-fluorescence of cytochrome P-450 in solution and liposomes from natural phosphatidylcholine, dimiristoylphosphatidylcholine, dipalmitoylphosphatidylcholine. The fluorescence spectrum of cytochrome P-450 is a superposition of triptophane and tyrosine components. During protein incorporation into liposomes a significant short-wave shift of the emission spectrum takes place. Temperature dependence of the intensity of cytochrome P-450 self-fluorescence in solution has bends at 30, 45 and 50 degrees C. When protein is incorporated into liposomes the location of bends depends on individual properties of lipids forming the bilayer. Effect of lipid surrounding on temperature conformational rearrangements of cytochrome P-450 molecule is discussed.
authors.forename: A A
authors.initials: AA
authors.lastname: Akhrem
authors.forename: S L
authors.initials: SL
authors.lastname: Romanov
authors.forename: V P
authors.initials: VP
authors.lastname: Karasev
authors.forename: M A
authors.initials: MA
authors.lastname: Kisel'
authors.forename: P A
authors.initials: PA
authors.lastname: Kiselev
mesh_terms: D003577:Cytochrome P-450 Enzyme System; D005453:Fluorescence; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D011487:Protein Conformation; D011510:Proteolipids; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D011510:Proteolipids; C020485:proteoliposomes; D003577:Cytochrome P-450 Enzyme System
vernacular_title: Temperaturnoe tushenie fluorestsentsii tsitokhroma P-450 v proteoliposomakh s razlichnym fizicheskim sostoianiem lipidnogo bisloia.