A simple technique for in situ embedding of monolayer cultures in Lowicryl K4M.
A number of difficulties are encountered in embedding monolayer cultures at low temperature in Lowicryl K4M resin. In this paper we present a simple procedure using Lab-Tek Flaskettes in which fixation, processing and embedding of monolayer cultures can readily be achieved.
Bou-Gharios
G
G
Department of Histopathology, Charing Cross and Westminster Medical School, London.
Adams
G
G
Moss
J
J
Shore
I
I
Olsen
I
I
D000180:Acrylic Resins; D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy / Q000379:methods*
D000180:Acrylic Resins; C037883:Lowicryl 4KM resin