Poly(rA) binding of alpha-anomeric and beta-anomeric tetrathymidylates covalently linked to an intercalating oxazolopyridocarbazolium. Determination of the binding parameters.
We have investigated by means of absorbance measurements at 310 nm the binding of alpha-anomeric or beta-anomeric tetrathymidylates covalently substituted at their 3' end by an intercalating agent (oxazolopyridocarbazolium), to poly(rA). Taking into account the strong autoaggregation of the free ligands, we have derived the binding parameters corresponding to the [alpha] and the [beta] ligands. The affinity of the alpha-anomer for poly(rA) is higher than the affinity of the beta-anomer in accordance with the Tm studies conducted on such a system.
Gautier
C
C
Laboratoire de Biochimie, INSERM U. 140, CNRS LA. 147, Institut Gustave-Roussy, Villejuif, France.
Bazile
D
D
Paoletti
J
J
D000470:Alkaloids; D004611:Ellipticines; D007364:Intercalating Agents; D007700:Kinetics; D008024:Ligands; D009838:Oligodeoxyribonucleotides; D011061:Poly A; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D013942:Thymine Nucleotides
D000470:Alkaloids; D004611:Ellipticines; D007364:Intercalating Agents; D008024:Ligands; D009838:Oligodeoxyribonucleotides; D013942:Thymine Nucleotides; D011061:Poly A