Use of a solid phase red blood cell adherence method for pretransfusion platelet compatibility testing.
A solid phase red blood cell adherence method has been used for platelet antibody detection and crossmatching for refractory platelet recipients. Patient sera were first screened for HLA or platelet-specific antibodies, then crossmatched with potential apheresis platelet donors. The overall correlation of platelet crossmatch results with transfusion outcome was 97% in patients with no evidence of nonimmune platelet destruction. The solid phase red blood cell adherence method provided a feasible and effective alternative to HLA matching as a means of donor selection for refractory platelet recipients. The speed and simplicity of this method may allow most hospital laboratories to perform platelet antibody screening before routine platelet transfusions.
Rachel
J M
JM
Community Blood Center of Greater Kansas City, Missouri 64111.
Summers
T C
TC
Sinor
L T
LT
Plapp
F V
FV
D000906:Antibodies / Q000032:analysis; D001781:Blood Component Removal; D001788:Blood Grouping and Crossmatching; D001792:Blood Platelets / Q000276:immunology*; D001803:Blood Transfusion; D002448:Cell Adhesion; D004912:Erythrocytes / Q000502:physiology*; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D017713:Platelet Transfusion; D011446:Prospective Studies
D000906:Antibodies; D006680:HLA Antigens