title: Detection of poultry and pork in cooked and canned meat foods by enzyme-linked immunosorbent assays.
abstract: Enzyme-linked immunosorbent assays (ELISA) are described for the detection of poultry and pork in cooked and canned meat foods. These assays are based on species-specific, polyclonal antibodies raised against heat-resistant antigens. The heat-resistant antigens were isolated from raw skeletal muscle tissue of pork and chicken and were found to be immunoreactive even after heating to 120 degrees C for 15 min. The poultry ELISA could detect chicken or turkey at the 126 ppm level, and the pork ELISA could detect pork at the 250 ppm level. Samples of frankfurters, bolognas, pressed meats, canned baby foods, and canned spreads were prepared by simple aqueous extractions.
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, Beltsville, MD 20705.
authors.forename: R G
authors.initials: RG
authors.lastname: Berger
authors.forename: R P
authors.initials: RP
authors.lastname: Mageau
authors.forename: B
authors.initials: B
authors.lastname: Schwab
authors.forename: R W
authors.initials: RW
authors.lastname: Johnston
mesh_terms: D000818:Animals; D001710:Biotin / Q000276:immunology; D002417:Cattle; D002645:Chickens; D003670:Deer; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D007614:Macropodidae; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D009132:Muscles / Q000032:analysis; D011202:Poultry Products / Q000032:analysis*; D012756:Sheep; D013552:Swine; D014422:Turkeys
chemical_list: D001710:Biotin