Aromatic ring cleavage of 4,6-di(tert-butyl)guaiacol, a phenolic lignin model compound, by laccase of Coriolus versicolor.
It was found that 2,4-di(tert-butyl)-4-(methoxycarbonylmethyl)-2-buten-4-ol ide (II) was formed as an aromatic ring cleavage product of a phenolic lignin model compound, 4,6-di(tert-butyl)guaiacol (I), by laccase of Coriolus versicolor. Based on isotopic experiments with 18O2 and H2 18O, the mechanism of formation of II from I is discussed.
Kawai
S
S
Research Section of Lignin Chemistry, Wood Research Institute, Kyoto University, Japan.
Umezawa
T
T
Shimada
M
M
Higuchi
T
T
D046508:Culture Techniques; D006139:Guaiacol / Q000031:analogs & derivatives; D006868:Hydrolysis; D042845:Laccase; D008031:Lignin / Q000378:metabolism*; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology
D006139:Guaiacol; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase