title: Identification of a putative structural gene for cathepsin D in Caenorhabditis elegans.
abstract: Mutants of Caenorhabditis elegans having about 10% of wild-type activity of the aspartyl protease cathepsin D have been isolated by screening. Mutant homozygotes have normal growth rates and no obvious morphological or developmental abnormalities. The mutant gene (cad-1) has been mapped to the right extremity of linkage group II. Heterozygous animals (cad-1/+) show intermediate enzyme levels and animals heterozygous for chromosomal deficiencies of the right extremity of linkage group II have 50% of wild-type activity. Cathepsin D purified from a mutant strain has a lower activity per unit mass of pure enzyme. These data suggest that cad-1 is a structural gene for cathepsin D.
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
authors.forename: L A
authors.initials: LA
authors.lastname: Jacobson
authors.forename: L
authors.initials: L
authors.lastname: Jen-Jacobson
authors.forename: J M
authors.initials: JM
authors.lastname: Hawdon
authors.forename: G P
authors.initials: GP
authors.lastname: Owens
authors.forename: M A
authors.initials: MA
authors.lastname: Bolanowski
authors.forename: S W
authors.initials: SW
authors.lastname: Emmons
authors.forename: M V
authors.initials: MV
authors.lastname: Shah
authors.forename: R A
authors.initials: RA
authors.lastname: Pollock
authors.forename: D S
authors.initials: DS
authors.lastname: Conklin
mesh_terms: D000818:Animals; D002107:Caenorhabditis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D002402:Cathepsin D / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D008040:Genetic Linkage; D006720:Homozygote; D009154:Mutation
chemical_list: D002402:Cathepsin D