Human and chick alpha 2(I) collagen mRNA: comparison of the 5' end in osteoblasts and fibroblasts.
Type I collagen, a heterotrimer of two alpha 1(I) chains and one alpha 2(I) chain, is the major structural protein of bone, skin, and tendon. The collagen of patients with bone diseases has been studied in skin fibroblasts instead of osteoblasts because the genes for type I collagen are single-copy genes. While these studies should detect structural changes in the gene, they might fail to detect defects in processes which are dependent on tissue-specific expression. The studies reported here sought to determine whether the expression of type I collagen in skin and bone was characterized by the use of alternate promoters or alternative splicing in the N-propeptide region. Primer extension and nuclease S1 protection experiments were used to analyze the structure of the alpha 2(I) mRNA from the 5' end of the gene through the N-telopeptide coding region (exons 1-6) in human and chick osteoblasts and fibroblasts. The protection and primer extension experiments using human and chick mRNA demonstrated identical routes of splicing in skin and bone at the first five splice junctions. These studies provide reassurance that information obtained from the study of type I collagen in fibroblasts may be extrapolated to bone.
Marini
J C
JC
Human Genetics Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Gottesman
G S
GS
Zasloff
M A
MA
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002642:Chick Embryo; D003094:Collagen / Q000235:genetics*; D003238:Connective Tissue / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D009928:Organ Specificity; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity
D012333:RNA, Messenger; D003094:Collagen