Colchicine, colcemide and cytochalasin B do not affect translocation of the glucocorticoid hormone-receptor in rat thymocytes or Ehrlich ascites cells.
The involvement of intracellular cytoskeletal elements in the translocation of the dexamethasone-receptor complex from the cytoplasm to the nucleus was studied using the cytoskeleton-disrupting agents colcemide, colchicine and cytochalasin B. These compounds did not affect the translocation of the hormone-receptor complex. We conclude that microfilaments and microtubules do not play a role in the translocation of the glucocorticoid hormone-receptor complex from the cytoplasm to the nucleus.
Vorgias
C E
CE
Max-Planck-Institut für Zellbiologie, Ladenburg, FRG.
Perides
G A
GA
Traub
P
P
Sekeris
C E
CE
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects; D003703:Demecolcine / Q000494:pharmacology*; D003907:Dexamethasone / Q000378:metabolism; D066298:In Vitro Techniques; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000187:drug effects* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D011965:Receptors, Glucocorticoid; C028697:dexamethasone receptor; D003571:Cytochalasin B; D003907:Dexamethasone; D003078:Colchicine; D003703:Demecolcine