Occurrence of three distinct molecular species of chondroitin sulfate proteoglycan in the developing rat brain.
More than 60% of brain chondroitin sulfate proteoglycans were extracted from 10-day-old rat brains by homogenization in ice-cold phosphate-buffered saline containing protease inhibitors. Although the soluble proteoglycan preparation was a mixture of chondroitin sulfate proteoglycans with a different hydrodynamic size as well as a different molecular density, each subfraction of the proteoglycans contained chondroitin sulfate side chains with virtually identical molecular weight (approximately 15,000) and chondroitin sulfate disaccharide composition (high content of 4-sulfate unit). Digestion of the purified proteoglycan preparation with protease-free chondroitinase ABC produced five core proteins with Mr = 250,000 (designated as 250K protein), 220,000 (220K), 150,000 (150K), 130,000 (130K), and 93,000 (93K). All these core proteins were obtained from chondroitin sulfate proteoglycan preparations extracted from various regions of the brain, but their composition varied among different brain regions. Analysis for amino acid composition of these core proteins and two-dimensional mapping of their proteolytic peptides revealed that three major core proteins (250K, 220K, and 150K proteins) were structurally different. These observations indicate that at least three distinct types of chondroitin sulfate proteoglycan occur in the developing rat brain.
Oohira
A
A
Department of Embryology, Institute for Developmental Research, Aichi, Japan.
Matsui
F
F
Matsuda
M
M
Takida
Y
Y
Kuboki
Y
Y
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D011508:Chondroitin Sulfate Proteoglycans / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010449:Peptide Mapping; D011509:Proteoglycans / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin; D014461:Ultracentrifugation
D000596:Amino Acids; D011508:Chondroitin Sulfate Proteoglycans; D011509:Proteoglycans; D014357:Trypsin