Simultaneous determination of imipramine, desipramine and their deuterium-labelled analogues in biological fluids by capillary gas chromatography-mass spectrometry.
A specific, sensitive and accurate method for the determination of imipramine, desipramine and their d4 analogues in biological fluids using d8 analogues as internal standards using capillary gas chromatography-chemical ionization mass spectrometry was developed. Drug concentrations were measured by selected-ion monitoring of the quasi-molecular ions of imipramine and the trifluoro-acetyl derivative of desipramine. The coefficient of variation and relative error at a concentration of about 2 ng/ml in plasma were found less than 10% and 7%, respectively, for both drugs. No isotope effect was observed following the oral administration of an equimolar mixture of imipramine and [2H4]imipramine to a rat.
Sasaki
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Y
Department of Physical and Pharmaceutical Chemistry, Tokyo College of Pharmacy, Japan.
Baba
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S
D000818:Animals; D002849:Chromatography, Gas; D003891:Desipramine / Q000032:analysis* / Q000097:blood / Q000652:urine; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007099:Imipramine / Q000032:analysis* / Q000097:blood / Q000652:urine; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007202:Indicators and Reagents; D003903:Deuterium; D007099:Imipramine; D003891:Desipramine