Blood glucose, lactate, pyruvate, glycerol, 3-hydroxybutyrate and acetoacetate measurements in man using a centrifugal analyser with a fluorimetric attachment.
Methods are described for the analysis of glucose, lactate, pyruvate, alanine, glycerol, 3-hydroxybutyrate and acetoacetate in perchloric acid extracts of human blood, using the Cobas Bio centrifugal analyser fitted with a fluorimetric attachment. Intra-assay and inter-assay coefficients of variation ranged from 1.9 to 7.9% and from 1.0 to 7.2% respectively. Correlation coefficients ranged from 0.96 to 0.99 against established continuous-flow and manual spectrophotometric methods. All seven metabolites can be measured using a single perchloric acid extract of 20 microliter of blood. The versatility of the assays is such that as little as 100 pmol pyruvate, 3-hydroxybutyrate or as much as 15 nmol glucose can be measured in the same 20 microliter extract.
Harrison
J
J
Department of Clinical Biochemistry and Metabolic Medicine, Medical School, Newcastle upon Tyne, UK.
Hodson
A W
AW
Skillen
A W
AW
Stappenbeck
R
R
Agius
L
L
Alberti
K G
KG
D000090:Acetoacetates / Q000097:blood; D001786:Blood Glucose / Q000032:analysis*; D002498:Centrifugation; D005470:Fluorometry; D005990:Glycerol / Q000097:blood*; D006801:Humans; D006885:Hydroxybutyrates / Q000097:blood*; D007773:Lactates / Q000097:blood*; D011773:Pyruvates / Q000097:blood*
D000090:Acetoacetates; D001786:Blood Glucose; D006885:Hydroxybutyrates; D007773:Lactates; D011773:Pyruvates; D005990:Glycerol