High-performance liquid chromatography of erythromycin propionyl ester and erythromycin base in biological fluids.
The simultaneous determination of erythromycin propionate and erythromycin base in serum and urine by high-performance liquid chromatography using oleandomycin as internal standard is described. The separation was achieved on a reversed-phase C18 column employing acetonitrile-0.05 M phosphate buffer (65:35), adjusted to pH 7.0, as the mobile phase with coulometric detection. Hydrolysis of the ester during blood sample collection was minimised by immediate high-speed centrifugation of collected blood samples, followed by separation and immediate freezing of the serum fraction. A solid-phase extraction procedure, combined with a simple phase-separation step was used prior to chromatographic analysis. The method has the necessary precision, sensitivity and accuracy to allow the simultaneous determination of both components in serum and urine following a single 500-mg oral dose of erythromycin estolate.
Stubbs
C
C
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Kanfer
I
I
D002851:Chromatography, High Pressure Liquid; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007202:Indicators and Reagents; D013607:Tablets
D007202:Indicators and Reagents; D013607:Tablets; D004917:Erythromycin; C011807:erythromycin propionate