Excision repair of pyrimidine dimers in human peripheral blood lymphocytes: comparison between mitogen stimulated and unstimulated cells.
Excision repair kinetics of UV-induced pyrimidine dimers in DNA of phytohemagglutinin (PHA)-stimulated human peripheral blood lymphocytes were compared to unstimulated lymphocytes using a dimer-specific endonuclease from Micrococcus luteus in conjunction with agarose gel electrophoresis. Removal of pyrimidine dimers could be detected within 6 h after irradiation only in PHA-stimulated lymphocytes. However, incorporation of [3H]thymidine as UV-induced unscheduled DNA synthesis was detected in the unstimulated lymphocytes in the 6-h period. The number of pyrimidine dimers remaining in unstimulated lymphocytes was approximately 85% after 24 h as compared to less than 25% in stimulated cells.
Freeman
S E
SE
Division of Biomedical Research, Lovelace Medical Foundation, Albuquerque, NM 87108.
Ryan
S L
SL
D002478:Cells, Cultured; D004247:DNA / Q000097:blood / Q000528:radiation effects*; D004260:DNA Repair; D004261:DNA Replication / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000528:radiation effects*; D011740:Pyrimidine Dimers / Q000097:blood*; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D004247:DNA