A rapid computerized method for quantitation of cellular mediated immunity in biological systems.
The aim of this study was to compare the quantitation of cellular mediated immunity (CMI) assessed by leukocyte migration (LM) using a Hewlett Packard Model 9825A Desk-top computer interfaced to a Talos Cyber Graphics Digitizer vs. the standard quantitation done by tracing the projected profile of the migrated leukocytes on papers, cutting and weighing. The computerized quantitation resulted in a percentage migration mean of 75.91 as compared to standard quantitation by weighing (76.37) (P greater than 0.05). The unweighted least squares linear regression resulted in R2 0.9987, an indication of a high correlation between the data obtained by both methods. The advantages of computerized quantitation of cellular mediated immunity are discussed.
Barbour
E K
EK
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Newman
J A
JA
Sasipreeyajan
J
J
D000818:Animals; D002465:Cell Movement; D002645:Chickens; D007962:Leukocytes / Q000276:immunology*; D008432:Mathematical Computing; D008838:Microcomputers; D012984:Software