Regulation of tubulin and actin synthesis and accumulation during Blastocladiella emersonii development.
Actin and alpha and beta-tubulin have been identified in Blastocladiella emersonii by two-dimensional gel electrophoresis and Western blotting. The kinetics of synthesis of these proteins were compared by pulse-labeling experiments with [35S]methionine and with the accumulation of their corresponding mRNAs, translated in a cell-free system. Large increases occur in the rates of actin and alpha- and beta-tubulin biosynthesis during sporulation and there is an accumulation of the corresponding mRNAs. In parallel to the increased synthesis, these cytoskeletal proteins accumulate during the late stage of sporulation.
da Silva
A M
AM
Departamento de Bioquímica, Universidade de São Paulo, Brazil.
Juliani
M H
MH
D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D001754:Blastocladiella / Q000378:metabolism / Q000502:physiology*; D008411:Chytridiomycota / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D013172:Spores, Fungal; D014404:Tubulin / Q000096:biosynthesis* / Q000302:isolation & purification
D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin