title: Quantitation of flurazepam and three metabolites by electron capture gas liquid chromatography.
abstract: Flurazepam and three of its metabolic products (desalkyl, hydroxyethyl, and aldehyde metabolites) can be simultaneously quantitated without derivatization by gas chromatography with electron capture detection. After addition of a suitable internal standard, unknown biological samples and calibration standards are extracted at neutral pH into benzene/isoamyl alcohol. The reconstituted extract is chromatographed at 275 degrees C with a 10% OV-101 liquid phase, which allows resolution of all 5 compounds. In some cases a 1% OV-225 liquid phase is used for quantitation of hydroxyethylflurazepam. The method is sufficiently sensitive and reproducible for use in clinical and experimental pharmacokinetic studies.
authors.affiliation: Department of Psychiatry, Tufts University School of Medicine, Boston, Massachusetts 02111.
authors.forename: E S
authors.initials: ES
authors.lastname: Burstein
authors.forename: H
authors.initials: H
authors.lastname: Friedman
authors.forename: D J
authors.initials: DJ
authors.lastname: Greenblatt
authors.forename: A
authors.initials: A
authors.lastname: Locniskar
authors.forename: H R
authors.initials: HR
authors.lastname: Ochs
mesh_terms: D000328:Adult; D002849:Chromatography, Gas; D005260:Female; D005479:Flurazepam / Q000032:analysis* / Q000378:metabolism; D006801:Humans
chemical_list: D005479:Flurazepam