Electroporation-mediated transfection of Acholeplasma laidlawii with mycoplasma virus L1 and L3 DNA.
In contrast to mycoplasma virus L1 and L2 circular DNA, mycoplasma virus L3 linear DNA is not biologically active in polyethylene glycol-mediated transfection. Electroporation of Acholeplasma laidlawii, however, leads to plaque formation after incubation with L3 DNA. The efficiency of electroporation-mediated transfection is 1/10 that of polyethylene glycol-mediated transfection as estimated with L1 DNA. Trypsin treatment of cells before DNA addition increases the efficiency of DNA uptake.
Lorenz
A
A
Abteilung Virologie, Institut für Mikrobiologie, Universität Ulm, Federal Republic of Germany.
Just
W
W
da Silva Cardoso
M
M
Klotz
G
G
D000128:Acholeplasma laidlawii / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D004560:Electricity; D008565:Membrane Proteins / Q000378:metabolism; D014162:Transfection; D014357:Trypsin / Q000378:metabolism
D004270:DNA, Circular; D004279:DNA, Viral; D008565:Membrane Proteins; D014357:Trypsin