title: Monoclonal antibody B72.3 reacts with a core region structure of O-linked carbohydrates.
abstract: Monoclonal antibody (moAb) B72.3 appears to be exceptional among the many mouse moAbs reactive with human tumor cells in its degree of tumor specificity. The antigen recognized was characterized as a mucin-like molecule. We report here that B72.3 reacts strongly with ovine submaxillary mucin and that reactivity is abolished by desialylation, suggesting that the determinant recognized is the disaccharide N-acetylneuraminic acid alpha (2----6)-N-acetylgalactosamine, linked to serine or threonine, which might be designated sialylated Tn. A variety of human mucin preparations, including human salivary mucins, and other glycoproteins containing O-linked or N-linked carbohydrates were nonreactive. The data suggest that the determinant recognized is a core structure which is normally not exposed due to chain elongation at C-3 of the N-acetylgalactosamine, which appears to be less frequent in carcinoma cells than in normal cells.
authors.affiliation: Center for Molecular Medicine and Immunology, Newark, N.J.
authors.forename: D V
authors.initials: DV
authors.lastname: Gold
authors.forename: M J
authors.initials: MJ
authors.lastname: Mattes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate; D002241:Carbohydrates / Q000276:immunology*; D002417:Cattle; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins / Q000032:analysis / Q000276:immunology*; D011402:Pronase / Q000494:pharmacology; D012756:Sheep
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D002241:Carbohydrates; D006023:Glycoproteins; D009077:Mucins; C040286:Tn antigen; D011402:Pronase