title: EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme.
abstract: A novel endoglucanase from Trichoderma reesei, EGIII, has been purified and its catalytic properties have been studied. The gene for that enzyme (egl3) and cDNA have been cloned and sequenced. The deduced EGIII protein shows clear sequence homology to a Schizophyllum commune enzyme (M. Yaguchi, personal communication), but is very different from the three other T. reesei cellulases with known structure. Nevertheless, all the four T. reesei cellulases share two common, adjacent sequence domains, which apparently can be removed by proteolysis. These homologous sequences reside at the N termini of EGIII and the cellobiohydrolase CBHII, but at the C termini of EGI and CBHI. Comparison of the fungal cellulase structures has led to re-evaluation of hypotheses concerning the localization of the active sites.
authors.affiliation: Biotechnical Laboratory, VTT, Espoo, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Saloheimo
authors.forename: P
authors.initials: P
authors.lastname: Lehtovaara
authors.forename: M
authors.initials: M
authors.lastname: Penttilä
authors.forename: T T
authors.initials: TT
authors.lastname: Teeri
authors.forename: J
authors.initials: J
authors.lastname: Ståhlberg
authors.forename: G
authors.initials: G
authors.lastname: Johansson
authors.forename: G
authors.initials: G
authors.lastname: Pettersson
authors.forename: M
authors.initials: M
authors.lastname: Claeyssens
authors.forename: P
authors.initials: P
authors.lastname: Tomme
authors.forename: J K
authors.initials: JK
authors.lastname: Knowles
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005796:Genes; D005800:Genes, Fungal; D003904:Mitosporic Fungi / Q000201:enzymology*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D004247:DNA; D002480:Cellulase