A beneficial effect of trypsin on the purification of turnip mosaic virus (TuMV) and other potyviruses.
The effects of treatment with trypsin during the purification of turnip mosaic virus (TuMV), on virus yield, infectivity and integrity of virus coat protein were examined. Trypsin increased yield markedly, and at a low concentration, increased infectivity. These effects were probably due to reduced aggregation of virus particles. At higher concentrations of trypsin, there was some degradation of virus coat protein, and infectivity was reduced. Treatment with trypsin at the optimum concentration can significantly improve purification of TuMV; more limited experiments suggest that it can also be applied to other potyviruses.
Thompson
S
S
Biochemistry and Molecular Biology Department, Institute of Horticultural Research, Wellesbourne, Warwick, U.K.
Fraser
R S
RS
Barnden
K L
KL
D002213:Capsid / Q000032:analysis; D007887:Fabaceae / Q000382:microbiology; D008854:Microscopy, Electron; D009029:Mosaic Viruses / Q000032:analysis / Q000302:isolation & purification*; D009149:Mustard Plant / Q000382:microbiology; D010940:Plant Proteins / Q000302:isolation & purification; D010946:Plants, Medicinal; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology*
D010940:Plant Proteins; D014357:Trypsin