The mechanism of experimental adipocere formation: hydration and dehydrogenation in microbial synthesis of hydroxy and oxo fatty acids.
The enzyme preparations from Flavobacterium meningosepticum solubilized by sonication catalyzed not only hydration of oleic acid to produce 10-hydroxystearic acid but dehydrogenation of this product. The mechanism of the hydration and dehydrogenation was proved by gas chromatography-mass spectrometry of 10-hydroxy and 10-oxostearic acids produced in the presence of D2O or H2(18)O. The activity of these enzymes was increased by preincubating Flavobacterium meningosepticum with oleic acid.
Gotouda
H
H
Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Takatori
T
T
Terazawa
K
K
Nagao
M
M
Tarao
H
H
D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000378:metabolism*; D005417:Flavobacterium / Q000201:enzymology / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006880:Hydroxy Acids / Q000378:metabolism*; D007651:Keto Acids / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D011180:Postmortem Changes
D005227:Fatty Acids; D006880:Hydroxy Acids; D007651:Keto Acids; D009829:Oleic Acids; D019301:Oleic Acid