Determination of avidin and streptavidin by a modified Bradford method.
Underestimation of avidin and streptavidin by the Bradford method can be alleviated by carrying out the reaction at 100 degrees C for 10-15 min, and the protein values thus obtained match very well with those of biotin-binding assays. "Thermal modification" is applicable to "standard" and "micro" versions of the method and has no effect on the spectral and stability characteristics of the protein-dye complexes.
Sharma
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Miles Inc., Cellular Diagnostics, Mishawaka, Indiana 46544.
Tihon
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D001360:Avidin / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D003124:Colorimetry; D004396:Coloring Agents; D008832:Microchemistry; D019809:Streptavidin; D013696:Temperature
D001426:Bacterial Proteins; D004396:Coloring Agents; D001360:Avidin; D019809:Streptavidin