Imidazole binding to human serum albumin.
Imidazole is a substance released by the organism when a new salicylate derivative, imidazole salicylate is administered. A study was made of the binding of imidazole to human serum albumin by an in vitro assay employing an ultrafiltration technique. For the concentration range that imidazole was found in plasma following administration of the drug to healthy volunteers, the mean binding percentages were: 12.1 +/- 1.8 and 19.7 +/- 3.1 at 37 degrees C and 25 degrees C, respectively. The results obtained in the study follow a model entailing three equal and independent binding sites of imidazole to serum albumin and the values of the corresponding constants were determined. Apparently, the presence in the plasma samples of sodium salicylate at a concentration of 100 micrograms/ml does not affect the binding of imidazole to human serum albumin.
Rodrigo
M C
MC
Physical Chemistry Department, Faculty of Pharmacy, University of Salamanca, Spain.
Ceballos
A
A
Mariño
E
E
Cachaza
J M
JM
Domínguez-Gil
A
A
Kuemmerle
H P
HP
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007093:Imidazoles / Q000097:blood* / Q000493:pharmacokinetics; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014462:Ultrafiltration
D007093:Imidazoles; D012709:Serum Albumin