title: K+-selective microelectrode study of internally dialyzed squid giant axons.
abstract: Intracellular potassium activity, (aK)i, and axoplasmic K+ concentration, [K+]i, were measured by means of K+-selective microelectrodes and atomic absorption spectroscopy, respectively, in squid giant axons dialyzed with K+-free dialysis solution and bathed in K+-free artificial sea water. (aK)i measurements indicated that axoplasmic free K+ could be depleted by dialysis, whereas [K+]i measurements on axoplasm extruded from these axons suggest substantial retention of K+ (15.5 +/- 1.7 mmol/kg axoplasm K+; n = 9). In comparison, [K+]i in axoplasm extruded from freshly dissected axons was 330 +/- 16 mmol/kg axoplasm (n = 6). These data suggest that approximately 5% of the axoplasmic K+ ions are not easily removed by dialysis and that these ions are either bound to macromolecular sites or sequestered into membrane-enclosed organelles.
authors.affiliation: Department of Physiology, University of Toronto, Ontario, Canada.
authors.forename: C N
authors.initials: CN
authors.lastname: Fong
authors.forename: D C
authors.initials: DC
authors.lastname: Chang
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D049832:Decapodiformes; D003956:Dialysis; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D011188:Potassium / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D011188:Potassium