title: Primary structure of a base non-specific ribonuclease from Rhizopus niveus.
abstract: The primary structure of a base non-specific ribonuclease from Rhizopus niveus (RNase Rh) was determined by nucleotide sequence analysis of the DNA fragment encoding RNase Rh gene including signal peptide sequence, and amino acid sequence analysis of the peptide obtained from RNase Rh and RNase Rh' (a protease-modified RNase Rh created during the course of purification). The sequence determined was: MKAVLALATLIGSTLASSCSSTA LSCSNSANSDTCCSPEYGLVVLNMQWAPGYGPANAFTLHGLWPDKCSGAYAPSGGCDSN RASSSIASVIKSKDSSLYNSMLTYWPSNQGNNNVFWSHEWSKHGTCVSTYDPDCYDNYE EGEDIVDYFQKAMDLRSQYNVYKAFSSNGITPGGTYTATEMQSAIESYFGAKAKIDCSSG TLSDVALYFYVRGRDTYVITDALSTGSCSGDVEYPTK (the sequence of signal peptide is underlined). The sequence indicates that the homology with the sequence of RNase T2 from A. oryzae with the same base specificity is about 42% and that the sequences around the two histidine residues which are supposed to be involved in the active site are fairly conserved.
authors.affiliation: Department of Agricultural Chemistry, University of Tokyo.
authors.forename: H
authors.initials: H
authors.lastname: Horiuchi
authors.forename: K
authors.initials: K
authors.lastname: Yanai
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: K
authors.initials: K
authors.lastname: Yano
authors.forename: E
authors.initials: E
authors.lastname: Wakabayashi
authors.forename: A
authors.initials: A
authors.lastname: Sanda
authors.forename: S
authors.initials: S
authors.lastname: Mine
authors.forename: K
authors.initials: K
authors.lastname: Ohgi
authors.forename: M
authors.initials: M
authors.lastname: Irie
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003062:Codon / Q000235:genetics*; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D012233:Rhizopus / Q000201:enzymology*; D012260:Ribonucleases / Q000032:analysis* / Q000235:genetics
chemical_list: D000596:Amino Acids; D003062:Codon; D012333:RNA, Messenger; D012260:Ribonucleases; D003488:Cyanogen Bromide