Electron microscopic localization of the Pa and RT1.Aa antigens on the placenta of the rat.
A major factor in the ability of the placenta to avoid allograft rejection is the differential expression of MHC class I antigens on its surface. Using monoclonal antibodies and the electron microscopic immunogold technique, we have demonstrated that only the pregnancy-associated (Pa) antigen, which carries a broadly shared antigenic determinant, is expressed on the placental surface in the rat, whereas the allele-specific classical transplantation antigens are not. Both types of antigens are, however, present in the cytoplasm of the basal trophoblast but completely absent from the labyrinthine trophoblast.
Kanbour
A
A
Department of Pathology, University of Pittsburgh School of Medicine, PA 15261.
Macpherson
T A
TA
Kunz
H W
HW
Gill
T J
TJ
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D005260:Female; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D015395:Histocompatibility Antigens Class I; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010920:Placenta / Q000276:immunology* / Q000648:ultrastructure; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D011257:Pregnancy Proteins; C038353:histocompatibility antigens RT, rat; C025625:pregnancy-specific antigen, sheep