[A new model for the regional perfusion of the extremities in rats: metabolism and hemodynamics].
A pelvic extremity of the rat was recirculatorily perfused by means of a self-developed perfusion equipment in a perfusion pressure of 65 +/- 6 mmHg and a blood temperature of 35 degrees C during one hour. Tissue specimens were taken away in the end of the perfusion and 3 weeks later and the metabolite contents was determined in comparison with control groups. No change of the metabolite status was provable by the perfusion. The technical practicability of the perfusion of rat extremities could be shown, therefore, contrary to hitherto existing assumptions.
Krüger
I
I
Chirurgischen Universitätsklinik Köln.
Ghussen
F
F
Nagel
K
K
Isselhard
W
W
D000818:Animals; D006439:Hemodynamics; D006614:Hindlimb / Q000098:blood supply; D008297:Male; D009132:Muscles / Q000098:blood supply / Q000378:metabolism / Q000502:physiology*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D013696:Temperature
Ein neues Modell zur regionalen Extremitätenperfusion der Ratte--Stoffwechsel und Hämodynamik.