[Synthesis of tetracontapeptide--a hypothetical evolutionary precursor of calcium-binding proteins. II. Condensation of fragments].
A tetracontapeptide was obtained by condensation of synthetic fragments (see the preceding paper) with the use of pentafluorophenyl esters as well as with carbodiimide and hydroxybenzotriazole. Racemization during fragment condensation was 1-2 per cent. Deblocking of the protected tetracontapeptide was carried out by treatment with trifluoroacetic acid and hydrogen fluoride with thioanisole. The obtained peptide was purified by gel-chromatography and HPLC.
Jung
R
R
Medvedkin
V N
VN
Mitin
Iu V
IuV
D005075:Biological Evolution; D002135:Calcium-Binding Proteins / Q000138:chemical synthesis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D010446:Peptide Fragments / Q000138:chemical synthesis*; D010455:Peptides / Q000138:chemical synthesis*; D011498:Protein Precursors / Q000138:chemical synthesis*; D013237:Stereoisomerism
D002135:Calcium-Binding Proteins; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors
Sintez tetrakontapeptida--gipoteticheskogo évoliutsionnogo predshestvennika kal'tsiĭsviazyvaiushchikh belkov. II. Kondensatsiia fragmentov.