Use of a conserved consensus oligomer in the identification of cytochrome P-450 mRNAs.
A region of high nucleic acid conservation present in many members of the phenobarbital (PB) family of cytochromes P-450 (P-450s) was targeted for the construction of an 18 base, eight-fold degenerate, consensus oligomer (the PB-mer). When utilized to screen a rabbit liver cDNA library, the PB-mer enabled the isolation of P-450 cDNAs. One isolate, designated pP-450 1a, was characterized by nucleotide sequence, as well as Northern and Southern blot analyses and was demonstrated to be a novel form. Primary nucleotide sequence indicates that this cDNA is highly homologous to several P-450 cDNAs, but only in the protein coding region. The 3'-noncoding segment of P-450 1a is longer and diverges markedly from known P-450 cDNAs in the rabbit PB family.
Department of Environmental Health, School of Public Health and Community Medicine, University of Washington, Seattle 98195.
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Hassett
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Omiecinski
D000595:Amino Acid Sequence; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000032:analysis; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D004247:DNA; D003577:Cytochrome P-450 Enzyme System