[Effects of ethyl alcohol ingestion on the disturbance of porphyrin metabolism by lead].
A study was made on the possibility of synergistic effects of ethyl alcohol and lead on porphyrin metabolism in rabbits. Experimental rabbits were divided into 4 groups. Group A was the control group not given any treatment, and the other 3 groups (Groups B, C and D) were treated with ethyl alcohol, lead, and ethyl alcohol and lead respectively, for 2 months. Ethyl alcohol solution (5%) was administered to rabbits in Groups B and D as drinking water on every weekday. The average dose of alcohol was 6 ml/kg/day (18 ml/cap/day). Lead was injected intravenously to rabbits in Groups C and D at a dose of 0.5 mg Pb/kg on alternate days (3 times per week). Furthermore, a large dose of Pb was administered to other rabbits (Group C'). In rabbits treated with alcohol alone (Group B), no effect was observed in the biochemical indicators related to porphyrin metabolism. In the groups treated with lead (Groups C and C') and with lead and alcohol combined (Group D), some biochemical changes in porphyrin metabolism developed with increase of Pb-B, i.e. increase of ALA-S activity and total porphyrin content in the bone marrow, elevation of FEP level, increase of ALA-U and CP-U, and decrease of ALA-D activity in erythrocytes. Comparison of Groups C and D showed that CP-U and ALA-U increased significantly in Group D, but no significant difference was observed between both groups in FEP and in ALA-S activity in the bone marrow and liver. The other laboratory measurements, such as total porphyrin contents in the liver and plasma, and GOT or GPT level in serum, showed no significant change in all the groups. In the present study, the biochemical changes suggesting synergism of lead and ethyl alcohol were observed slightly in ALA-U and CP-U but not in ALA-S and FEP. These results suggest that these changes are essentially due to lead rather than mutual enhancement of the direct effects of these two toxins on porphyrin metabolism. However, it still remains to be determined whether or not ethyl alcohol affects the liver and kidney functions which may be related to ALA and CP excretion.
Ohmori
S
S
Department of Hygiene, Kumamoto University Medical School.
Harada
K
K
Miyakita
T
T
Miura
H
H
D000624:5-Aminolevulinate Synthetase / Q000378:metabolism; D000622:Aminolevulinic Acid / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D004357:Drug Synergism; D000431:Ethanol / Q000493:pharmacokinetics / Q000494:pharmacology*; D007854:Lead / Q000493:pharmacokinetics / Q000633:toxicity*; D008099:Liver / Q000378:metabolism; D008297:Male; D000623:Porphobilinogen Synthase / Q000378:metabolism; D011166:Porphyrins / Q000378:metabolism*; D011817:Rabbits
D011166:Porphyrins; D007854:Lead; D000431:Ethanol; D000622:Aminolevulinic Acid; D000624:5-Aminolevulinate Synthetase; D000623:Porphobilinogen Synthase