Determination of hydrogen peroxide in beverages by high-performance liquid chromatography with fluorescence detection.
A high-performance liquid chromatographic method is described for the determination of hydrogen peroxide in beverages. The method involves the enzymatic conversion of hydrogen peroxide into formaldehyde by catalase-methanol, followed by the derivatization of formaldehyde with 4-amino-3-penten-2-one. The reaction was carried out under the flow of nitrogen, which allows the detection of hydrogen peroxide without interference from reducing substances such as ascorbic acid. More than 90% of hydrogen peroxide spiked at the 2 ppm level was recovered from several beverages. The method exhibits good linearity, accuracy and precision, and the minimum detectable level was 0.05 ppm.
Hamano
T
T
Public Health Research Institute of Kobe City, Japan.
Mitsuhashi
Y
Y
Yamamoto
S
S
D001205:Ascorbic Acid / Q000032:analysis; D001628:Beverages / Q000032:analysis*; D002374:Catalase; D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D000432:Methanol / Q000032:analysis; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D006861:Hydrogen Peroxide; D002374:Catalase; D001205:Ascorbic Acid; D000432:Methanol