Trans-splicing in chloroplasts: the rps 12 loci of Nicotiana tabacum.
The rps12 gene in tobacco chloroplasts consists of three exons that code for a polypeptide with homology to Escherichia coli ribosomal protein S12. C-terminal exons 2 and 3 of rps12 are located in the inverted repeat regions of the tobacco chloroplast genome. Exon 1 of rps12 is 29 kilobase pairs downstream of the nearest copy of exons 2 and 3 and 69 kilobase pairs away from the distal copy of exons 2 and 3. RNA gel blot hybridization analysis and primer extension sequencing of cDNA to rps12 encoding RNAs indicate that exon 1 and exons 2 and 3 are encoded on separate transcripts. Exon 1 and exons 2 and 3 are covalently ligated in the correct reading frame in rps12 mRNA. These results indicate that a bimolecular (trans-) splicing event occurs during the formation of mature rps12 mRNA.
Department of Biochemistry, University of Arizona, Tucson 85721.
M
M
Hildebrand
R B
RB
Hallick
C W
CW
Passavant
D P
DP
Bourque
D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D005091:Exons; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D014026:Nicotiana / Q000235:genetics
D010940:Plant Proteins; D012333:RNA, Messenger