Determination of chloramphenicol in swine muscle tissue using a monoclonal antibody-mediated clean-up procedure.
A rapid and specific clean-up procedure based on immunoaffinity chromatography for the high-performance liquid chromatographic determination of chloramphenicol (CAP) in swine muscle tissue at the 10 micrograms kg-1 level is described. For preparation of the columns, monoclonal antibodies against CAP were coupled to cyanogen bromide-activated Sepharose. After immunosorption of CAP from the aqueous meat extract, CAP was eluted with methanol. Mean recoveries from spiked swine muscle tissue were 70 +/- 6% (10-50 micrograms kg-1 spiking levels). The loss of CAP can be completely attributed to the extraction; no loss was observed during the antibody-mediated clean-up.
Faculty of Veterinary Medicine, Department of the Science of Food of Animal Origin, University of Utrecht, The Netherlands.
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C
van de Water
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Haagsma
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002701:Chloramphenicol / Q000032:analysis*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000032:analysis*; D013552:Swine
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D002701:Chloramphenicol