Application of the GTGO-AD procedure in the scanning electron microscopy of streptococci adhered to pellicle-coated dental enamel.
The scanning electron microscope (SEM) offers a direct and visually effective means of examining streptococci adhering to pellicle-coated dental enamel. The GTGO-AD (Glutaraldehyde-Tannic acid-Guanidine hydrochloride-Osmium tetroxide-Air-Drying procedure) has the advantage that critical point-drying or freeze-drying need not be employed. It was found that both the structural and spatial integrity of the adherent streptococci and the pellicle were maintained with the GTGO-AD procedure.
Edwards
P J
PJ
Beecham Products Research Department, Weybridge, Surrey.
Jeffryes
C A
CA
D001422:Bacterial Adhesion; D003743:Dental Enamel / Q000382:microbiology* / Q000648:ultrastructure; D044622:Dental Pellicle; D006023:Glycoproteins; D006801:Humans; D007202:Indicators and Reagents; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008963:Molar; D013291:Streptococcus / Q000302:isolation & purification / Q000648:ultrastructure*
D006023:Glycoproteins; D007202:Indicators and Reagents