Origin and evolution of a major feline satellite DNA.
A major satellite DNA has been cloned from the domestic cat (Felis catus) and characterized. The satellite monomer, termed FA-SAT, is 483 base-pairs in size, 64% G + C, and represents about 1 to 2% of the cat genome. A consensus sequence based upon partial sequence data from 21 independently isolated clones demonstrates: (1) FA-SAT is not composed of a series of shorter repeats, although about 25 copies, primarily imperfect, of the hexanucleotide TAACCC appear in the sequence; (2) there are many more CpG dinucleotides present in FA-SAT than expected for a random sequence of its size; and (3) 61% of all base substitutions in FA-SAT involve the replacement of G and C residues by A and T residues, indicating that FA-SAT is rapidly becoming A + T-rich. FA-SAT-related sequences are found in many mammals, where they appear to be scattered throughout the genome and not tandemly arranged as in the cat. An FA-SAT-related sequence was cloned from the domestic dog genome and sequenced, and shown to contain multiple copies of the same TAACCC hexanucleotide found in the cat satellite.
Fanning
T G
TG
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002415:Cats; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D004285:Dogs; D006168:Guinea Pigs; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite