Preferentially expressed genes in stomach adenocarcinoma cells.
cDNA clones complementary to mRNA of neoplastic cells of human stomach tissue were used to examine quantitative changes in the mRNA levels of specific genes in neoplastic cells. Poly(A)+ RNA from poorly differentiated adenocarcinoma cells of a female patient with stomach cancer was used for construction of a complementary DNA (cDNA) library. Screening of the 18,000 colonies utilizing 32P-cDNAs derived from normal human tissue and stomach carcinoma tissue samples was used to select clones likely to represent sequences preferentially expressed in stomach carcinoma cells. Twenty-six recombinants were initially selected and further analysis of these clones indicated that eight (4-3D, 9-2D, 9-4G, 29-1G, 29-6F, 37-1B, 115-5A and 52-5F) contain sequences preferentially expressed in stomach carcinoma cells. We have identified the 9-4G, 29-1A, and 29-6F genes which are differentially expressed in human neoplasia.
First Department of Internal Medicine, School of Medicine, Ehime University, Japan.
T
T
Shiosaka
Y
Y
Tanaka
Y
Y
Kobayashi
D000230:Adenocarcinoma / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D003110:Colonic Neoplasms / Q000235:genetics; D004270:DNA, Circular; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis; D013274:Stomach Neoplasms / Q000032:analysis / Q000235:genetics*; D014158:Transcription, Genetic
D004270:DNA, Circular; D012333:RNA, Messenger; D012334:RNA, Neoplasm