Solid phase reverse passive hemadsorption test for hepatitis B surface antigen.
A solid phase reverse passive hemadsorption test (SP-RPHAd) for hepatitis B surface antigen detection is described. It was compared with a commercial reverse passive hemagglutination assay (Hepatest, Wellcome, U.K.). SP-RPHAd is four-fold less expensive than Hepatest and undiluted sera can be used instead of eight-fold diluted sera without risk of non-specific hemagglutination. Thus, the threshold of detection is lowered to about 5 ng/ml by SP-RPHAd.
Laboratoire de Virologie, Centre Hospitalo-Universitaire, Amiens, France.
G
G
Duverlie
M
M
Driencourt
C
C
Roussel
P
P
Daniel
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006382:Hemadsorption; D006386:Hemagglutination Tests / Q000379:methods; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans
D006514:Hepatitis B Surface Antigens