The bulk of ATP is associated to proteins in the living cell: a release kinetics study.
The analysis of the nonionic detergent induced release kinetics of intracellular components is a promising approach to obtain information on the possible molecular interactions inside the living cell. The molecular interactions of ATP were studied by monitoring the nonionic detergent-induced release kinetics of ATP from cultured human cells. The observed rapid mobilization rate in the presence of Triton X 100 and the retarded release in the presence of Brij 58 were in good agreement with the detergent-induced release kinetics of proteins and K+ reported earlier. These data are consistent with the idea that the bulk of ATP is associated to the protein macromolecules of the living cell.
Department of Clinical Chemistry, University Medical School, Pecs, Hungary.
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T
Koszegi
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Kellermayer
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Berenyi
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K
Jobst
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CF
Hazlewood
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D003902:Detergents / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
D003902:Detergents; D011506:Proteins; D000255:Adenosine Triphosphate