Sperm wash in three culture media: maximization of motile sperm recovery during swim-up incubation.
Three different culture media commonly used during in vitro gamete manipulations were studied for their efficacy in sperm wash procedure. Highest numbers of motile sperm were recovered at 6 hours following incubation in WT-6 and Ham's F-10 media. However, WT-6 yielded higher motile sperm numbers than Ham's F-10. Swim-up sperm number reached a peak at 3 hours following incubation in BWW. A period of 2 to 6 hours of incubation of sperm pellets overlayed with sperm wash media resulted in highly enriched motile sperm fractions free of dead spermatozoa and seminal debri.
Vijayakumar
R
R
Department of Obstetrics and Gynecology, School of Medicine, Texas Tech University Health Sciences Center, Lubbock.
Ndubisi
B
B
De Leon
F
F
Heine
W
W
D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*
D003470:Culture Media