Aldosterone induces chloride transport in the cortical collecting tubule.
The ontogenetic differentiation of transepithelial chloride transport was evaluated in the cortical collecting tubule of the rabbit kidney. Tubules from four control groups (I-IV) were studied during in vitro perfusion. I: body weight 150-280 g; II: 330-480 g; III: 530-880 g; IV: 980-1610 g. In each group, aldosterone (100 micrograms/100 g body weight/day) was given subcutaneously in three doses daily, for 6 days (IA-IVA). Transepithelial net chloride flux (pmol cm2 s1) increased by a factor of almost 3 from group I to group IV (p less than 0.01). Aldosterone induces net chloride flux by 103% (P = 0.03) in IA and by 78% (P = 0.01) in IIA; changes in groups III (21%) and IV (27%) were small. Therefore, the mineralocorticoid induces transepithelial chloride transport in cortical collecting tubule during early transport differentiation. The inducing action decreases with natural differentiation. Moreover, aldosterone alone suffices to induce the complete expression of transepithelial chloride transport in the cortical collecting tubule.
Nasemann
J
J
Physiologisches Institut der Universität München, F.R.G.
Horster
M F
MF
D000375:Aging; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002712:Chlorides / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D007684:Kidney Tubules / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D007700:Kinetics; D010042:Ouabain / Q000494:pharmacology; D010477:Perfusion; D011817:Rabbits; D012016:Reference Values
D002712:Chlorides; D000450:Aldosterone; D010042:Ouabain