On the separation ability of various Ficoll gradient solutions in zonal centrifugation.
Ficoll samples of various molecular weights, Mr(15-4200) X 10(3), forming gradient solutions with different separation resolutions and a more selective action have been obtained by fractionation of Ficoll 400 polysaccharide. On the basis of sedimentation-diffusion data and viscometry of Ficoll fractions in water and dimethylformamide, conclusions about the type of branching of macromolecules and the intensity of intramolecular hydrodynamic interaction were made; the size and shape asymmetry of Ficoll molecules were calculated in connection with their mobility in solution.
Institute of Macromolecular Compounds of the Academy of Sciences of the USSR, Leningrad.
P N
PN
Lavrenko
O I
OI
Mikriukova
O V
OV
Okatova
D002501:Centrifugation, Zonal / Q000295:instrumentation*; D004058:Diffusion; D005362:Ficoll; D008970:Molecular Weight; D011134:Polysaccharides; D014783:Viscosity
D011134:Polysaccharides; D005362:Ficoll