Differences in phosphorylation of isolated phospholamban in dependence on the preparation procedure.
Phospholamban was purified by two different preparation protocols. The products of both preparations are immunochemical similar. They differ, however, with regard to their phosphorylation by the catalytic subunit of cAMP-dependent protein kinase. It is suggested that the lipid environment plays a crucial role in the exposition of the phosphorylation sites of phospholamban.
Central Institute for Cardiovascular Research, Acad. Sci. GDR, Berlin.
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G
Grunow
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M
Holtzhauer
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H
Sydow
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H
Will
D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008081:Liposomes; D009206:Myocardium / Q000032:analysis; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000032:analysis; D013552:Swine; D000099211:Phospholamban
D002135:Calcium-Binding Proteins; D008081:Liposomes; D011494:Protein Kinases; D000099211:Phospholamban