Mouse placental 57-kDa calcium-binding protein: II. Localization of mRNA in mouse and human placentae by in situ cDNA hybridization.
Using a cloned cDNA to the mouse placental calcium-binding protein (MCaBP) [Tuan and Kirwin, preceding paper], mRNA transcripts of the MCaBP have been localized in the mouse placenta by in situ cDNA-RNA hybridization. The procedure involved the use of a nonradioactive, biotinylated cDNA probe. Paraffin sections of the mouse chorioallantoic placenta were processed for in situ hybridization using a procedure modified from that of Lo [8]. The results demonstrated that MCaBP mRNA is localized specifically to the fetal trophoblast of the mouse placenta, and is expressed in a development-dependent manner. The MCaBP cDNA also hybridized strongly in situ to sections of human term placenta, giving rise to signals localized specifically to the syncytiotrophoblastic layer of the chorionic villi.
Department of Biology, University of Pennsylvania, Philadelphia 19104.
R S
RS
Tuan
B T
BT
Lamb
C B
CB
Jesinkey
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D004247:DNA; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011257:Pregnancy Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*
D002135:Calcium-Binding Proteins; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D004247:DNA