In vitro insulin-like actions of human growth hormone: a study with an insulin antibody.
When polyclonal insulin antibodies were preincubated with either adipose tissue from hypophysectomized rats or adipocytes from normal rats, human growth hormone failed to stimulate glucose oxidation. Removal of insulin from adipocytes through incubation with pyruvate at pH 7.0, followed by washing three times, also abolished subsequent in vitro insulin-like action of hGH. Administration of the same insulin antibody to hypophysectomized rats 30 minutes prior to injection of hGH did not inhibit the insulin-like activity of the hGH as measured by its ability to decrease serum glucose and non-esterified fatty acid levels. It is concluded that the in vitro promotion of glucose oxidation by hGH requires insulin. Because of the uncertainty of complete removal of insulin in intact animals, such a conclusion cannot be made regarding in vivo insulin-like action of hGH.
Whittier Institute for Diabetes and Endocrinology, La Jolla, California.
L G
LG
Frigeri
K
K
Teguh
U J
UJ
Lewis
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D003429:Cross Reactions; D005947:Glucose / Q000378:metabolism; D013006:Growth Hormone / Q000276:immunology / Q000494:pharmacology*; D007016:Hypophysectomy; D066298:In Vitro Techniques; D007330:Insulin Antibodies / Q000276:immunology*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013002:Somatomedins / Q000276:immunology
D007330:Insulin Antibodies; D013002:Somatomedins; D013006:Growth Hormone; D005947:Glucose