Differentiation of Leishmania strains of Indian origin by lectin-mediated agglutination.
Characterisation of surface saccharides was carried out in 3 different strains of Indian leishmaniae by 7 lectins. Energy dependent agglutination and role of cytoskeletal disintegrator were also analysed. Wisteria floribunda, peanut agglutinin and soybean agglutinin can be used to differentiate clearly the leishmanial strains causing disease like visceral leishmaniasis, post Kala-azar dermal leishmaniasis and cutaneous leishmaniasis. The remaining lectins have mixed agglutination behaviour towards these strains. The effective concentration of dinitrophenol, sodium azide and colchicine is 10 mM, 25 mM and 1.25 x 10(-2) M, respectively.
Ghosh
D K
DK
Department of Zoology, University of Calcutta, India.
Ghosh
A K
AK
Bhattacharya
A
A
D000372:Agglutination Tests; D000818:Animals; D007194:India; D037102:Lectins; D007893:Leishmania donovani / Q000302:isolation & purification*; D007895:Leishmania tropica / Q000302:isolation & purification*
D037102:Lectins