Aldehyde dehydrogenase activity and vascular disease in type II diabetes--a comparison between 2 different assays for activity.
We have recently reported that type II diabetic subjects with macroangiopathy have a higher activity of aldehyde dehydrogenase (ALDH) in blood than those without clinical vascular disease. ALDH activity was measured as the elimination of acetaldehyde added to a blood homogenate in vitro. We have re-examined our clinical material with another assay of ALDH which uses indole-3-acetaldehyde as substrate and measures the formation of indole-3-acetic acid. A negative correlation between the half-life of acetaldehyde and the formation of indole-3-acetic acid was found in the group of subjects free from vascular disease (r = -0.55, p less than 0.01). Thus, a rapid elimination of acetaldehyde corresponded to a rapid formation of indole-3-acetic acid. No such correlation was found in subjects with macroangiopathy. These results suggest that the 2 groups, with and without clinical vascular disease, have differences in isoenzyme composition, in the kinetic properties of the enzyme, or in the non-enzymatic binding of acetaldehyde.
Department of Medicine, University of Lund, General Hospital, Malmö, Sweden.
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H
Ohlin
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Helander
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Tottmar
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Jerntorp
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Almér
D000444:Aldehyde Dehydrogenase / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology*; D003925:Diabetic Angiopathies / Q000201:enzymology*; D006801:Humans; D007210:Indoleacetic Acids / Q000032:analysis; D007211:Indoles
D007210:Indoleacetic Acids; D007211:Indoles; C001655:indole-3-acetaldehyde; C030737:indoleacetic acid; D000444:Aldehyde Dehydrogenase