The regulation of expression of human beta-globin genes.
We have analyzed the regulation of expression of the human beta-globin gene by DNA mediated gene transfer in MEL cells and microinjection in fertilized mouse eggs. The results show that the beta-globin gene contains two regulatory elements: one element in the promoter, that is required for full transcriptional activity and, secondly, a tissue and developmental specific enhancer 3' to the gene.
Laboratory of Gene Structure and Expression, National Institute for Medical Research, Mill Hill, London, U.K.
F
F
Grosveld
M
M
Antoniou
G B
GB
van Assendelft
E
E
de Boer
J
J
Hurst
G
G
Kollias
F
F
MacFarlane
N
N
Wrighton
D000818:Animals; D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D011401:Promoter Regions, Genetic; D013045:Species Specificity; D014162:Transfection
D005914:Globins