Proteins induced by Boolarra virus in Drosophila melanogaster line 1 cells.
In Boolarra virus infected Drosophila line 1 cells five virus-induced proteins were readily detected 20 h postinfection. These were A (103,500), alpha (46,000), beta (40,000), B (9,600), and gamma (6,600) and are similar in molecular weight to those induced in vivo by other nodaviruses. The addition of actinomycin D for the 1st h before the labeling period resulted in the production of all five proteins; but when actinomycin D was introduced for the entire labeling period, the synthesis of the products of RNA1 was greatly reduced. Host functions inhibited by actinomycin D may play a major role in the replication of this virus.
Department of Microbiology, Monash University Medical School, Prahran, Vic., Australia.
J B
JB
Bashiruddin
J L
JL
Martin
D000818:Animals; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D007304:Insect Viruses / Q000187:drug effects / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014779:Virus Replication / Q000187:drug effects
D014764:Viral Proteins; D003609:Dactinomycin