Isolation and purification of fibrinogen/fibrin degradation products by chromatography on protamine-agarose.
Protamine-agarose chromatography is introduced as a rapid and efficient method for the isolation of fibrinogen/fibrin degradation products from biological fluids such as human plasma. All fibrinogen/fibrin fragments above a MW of 35000, including fragments D and E are quantitatively adsorbed to insolubilized protamine and can be eluted with a buffer containing 0.2 M sodium citrate/citric acid pH 5.3, following previous elution of non-fibrinogen proteins with a buffer containing 0.8 M NaCl at neutral pH. Fragments D and E are separated by stepwise elution. The efficiency of the method is - evaluated by applying it to plasma samples obtained from healthy donors and from patients with clinical and laboratory evidence of disseminated intravascular coagulation.
Fakultät für klinische Medizin Mannheim der Universität Heidelberg, Mannheim, Germany.
C E
CE
Dempfle
D L
DL
Heene
D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005337:Fibrin / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D005340:Fibrinogen / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D011479:Protamines
D011479:Protamines; D005337:Fibrin; D005340:Fibrinogen