Effects of 1,1,1-trichloroethane on synaptosomal calcium accumulation in mouse brain.
Addition of 1,1,1-trichloroethane (TCE), in vitro to synaptosomes isolated from the mouse cerebellum and cerebral cortex inhibited the slow phase of K+-stimulated 45Ca2+ influx and the net 45Ca2+ influx (delta k). In the brain stem, however, TCE increased the fast and slow phases of calcium uptake under depolarizing conditions and also delta k. The non-depolarized calcium influx was not altered by TCE added in vitro. Two hours after injection of TCE (2.4 g/kg) the calcium accumulation in the presence of high K+ was lowered in the cerebellar synaptosomes, while it was increased in brain stem synaptosomes. TCE administered in vivo did not alter the calcium influx into cerebrocortical synaptosomes nor did it affect the non-depolarization-induced 45Ca2+ influx. Thus, these data indicate that TCE may influence voltage-dependent calcium channels in mouse brain synaptosomes.
Department of Toxicology, National Institute of Occupational Health, Solna, Sweden.
K B
KB
Nilsson
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D011188:Potassium / Q000494:pharmacology; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014241:Trichloroethylene / Q000633:toxicity*
D002132:Calcium Radioisotopes; D014241:Trichloroethylene; D011188:Potassium; D002118:Calcium