[125I]amatoxin and anti-amatoxin for radioimmunoassay prepared by a novel approach: chemical and structural considerations.
A novel approach for the production of [125I]amatoxin and anti-amanitin is described. The antigen and the starting material for Bolton Hunter iodination is prepared by periodic acid oxidation of the gamma-delta-dihydroxy-isoleucine side chain of alpha-amanitin followed by reductive amination. The antigen seems to be of low apparent toxicity. Chemical, spectroscopic and immunological evidence suggests that the hapten has a secondary structure similar to alpha-amanitin, but a modified tryptophanyl ring system. The establishment of a clinically useful iodinated radioimmunoassay system is possible, because the ring system seems to be of limited immunological importance.
Swiss Federal Institute for Reactor Research, Wuerenlingen.
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Andres
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Frei
D000546:Amanitins / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet; D013388:Succinimides
D000546:Amanitins; D000906:Antibodies; D013388:Succinimides; C018207:amatoxin; C022581:Bolton-Hunter reagent