First derivative spectrophotometric determination of anhydrotetracyclines in tetracyclines.
A rapid method is presented for detection and determination of anhydrotetracycline-HCl (ATC-HCl) and 4-epianhydrotetracycline-HCl (4-EATC-HCl) in tetracycline-HCl (TC-HCl). The method determines the 2 compounds as a sum, not individually. The first derivative absorption curve has a trough (D1) at 460 nm which is linearly related to concentration (1-10 mg/mL). ATC-HCl + 4-EATC-HCl content was determined in TC-HCl powder and capsules by the D1 and the compensation D1 spectrophotometric methods. The results were compared with those obtained using U.S. Pharmacopeia and British Pharmacopoeia methods.
King Saud University, College of Pharmacy, Pharmaceutical Chemistry Department, Riyadh, Saudi Arabia.
A A
AA
Wahbi
H A
HA
Al-Khamees
A M
AM
Yousef
D055598:Chemical Phenomena; D002621:Chemistry; D002751:Chlortetracycline / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013752:Tetracycline / Q000032:analysis*; D013754:Tetracyclines / Q000032:analysis*
D013754:Tetracyclines; C016229:4-epianhydrotetracycline; D013752:Tetracycline; D002751:Chlortetracycline