Experimental ischemia induces cell mitosis in the adult rat retina.
Experimental occlusion of the central retinal vessels in albino rats followed by reperfusion for 2 or 4 days resulted in a highly active proliferative response. Mitotic figures were produced in retinal vascular cells, presumed Müller cells, retinal pigment epithelial cells, and possibly other cell populations in the retina. A large number of mitoses were observed in 60-100% of 50 experimental eyes with 2, 4 or 6 hr of experimental ischemia and 2 or 4 days of reperfusion and in none of the 37 control eyes. Autoradiography demonstrated 3H-thymidine uptake in the retina and in vascular cells on a trypsin digest preparation of the retina. Retinal ischemia followed by reperfusion leads to mitosis of retinal cells. This suggests a causal relationship in proliferative retinopathies where ischemia and cell proliferation coincide.
National Eye Institute, Bethesda, Maryland.
E
E
Stefánsson
C A
CA
Wilson
T
T
Schoen
T
T
Kuwabara
D000818:Animals; D001345:Autoradiography; D001775:Blood Circulation; D005123:Eye / Q000378:metabolism / Q000473:pathology; D005260:Female; D006168:Guinea Pigs; D007511:Ischemia / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D008938:Mitosis; D010857:Pigment Epithelium of Eye / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000473:pathology*; D012171:Retinal Vessels / Q000503:physiopathology; D013936:Thymidine / Q000378:metabolism
D013936:Thymidine