Muscle D-glyceraldehyde-3-phosphate dehydrogenase from Anas sp.--1. Purification and properties of the enzyme.
Muscle D-GPDH from Anas sp. ws isolated and purified. Basic kinetic constants and physico-chemical studies are reported. The enzyme is a tetramer with a mol. wt of 140,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis gave s single band with a mol. wt of approx. 36,000. The other catalytic properties of the enzyme are discussed relative to those of enzyme obtained from other sources.
Barbosa
V M
VM
Department of Biochemistry, University of Paraná, Brasil.
Nakano
M
M
D000818:Animals; D002852:Chromatography, Ion Exchange; D004372:Ducks / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases