Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg.
A wild-type strain of Methanobacterium thermoautotrophicum Marburg was transformed by DNA from strains resistant to 5-fluorouracil. Recipient cells were grown without selection on gellan gum (GELRITE) plates with DNA. Drug-resistant cells were recovered by replica plating the resulting colonies onto drug plates. Transformation required high-molecular-weight DNA with appropriate markers and was not observed on agar or in liquid media under a variety of conditions.
Department of Botany and Microbiology, University of Oklahoma, Norman 73019.
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Worrell
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Nagle
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McCarthy
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Eisenbraun
D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D019605:Euryarchaeota / Q000187:drug effects / Q000235:genetics*; D005472:Fluorouracil / Q000494:pharmacology; D005819:Genetic Markers; D009154:Mutation; D010641:Phenotype; D014169:Transformation, Bacterial
D003470:Culture Media; D004269:DNA, Bacterial; D005819:Genetic Markers; D005472:Fluorouracil