Detection of anticentromere antibodies using cloned autoantigen CENP-B.
A solid-phase enzyme-linked immunosorbent assay has been established using a cloned fusion protein, CtermCENP-B [beta-gal], as antigen. The fusion protein carries the major epitope of CENP-B, the major centromeric autoantigen. The enzyme-linked immunosorbent assay was more sensitive than immunofluorescence techniques in detecting anticentromere antibodies in patients with scleroderma or Raynaud's disease, and was weakly positive in 3% of normal controls and in 3% of 70 patients with other connective tissue diseases.
Rothfield
N
N
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
Whitaker
D
D
Bordwell
B
B
Weiner
E
E
Senecal
J L
JL
Earnshaw
W
W
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D002503:Centromere / Q000276:immunology*; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D002875:Chromosomes / Q000276:immunology*; D003001:Cloning, Molecular; D003240:Connective Tissue Diseases / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D008875:Middle Aged; D009698:Nucleoproteins / Q000276:immunology*
D001323:Autoantibodies; D001324:Autoantigens; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D009698:Nucleoproteins