Purification and characterization of carcinoembryonic antigen from GW-39, a xenografted human colonic tumor system.
Carcinoembryonic antigen (CEA) was purified from GW-39 human tumor xenografts in hamsters by immunoaffinity chromatography. Binding of the antigen to immobilized monoclonal antibody provided a high degree of purification of CEA in a single step. A recovery of 79% and a 750-fold purification were obtained. The purified CEA has a molecular size of 180 kilodaltons, an isoelectric point of 4.4, and a specific activity of 0.94. About 73% of the radiolabeled GW-39 CEA reacted with goat anti-CEA serum. The amino acid and carbohydrate compositions of the GW-39 CEA were comparable to those of CEA preparations from other sources.
Center for Molecular Medicine and Immunology, University of Medicine and Dentistry of New Jersey, Newark.
Y Y
YY
Tu
F J
FJ
Primus
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D
Shochat
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DM
Goldenberg
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003110:Colonic Neoplasms / Q000276:immunology*; D006224:Cricetinae; D006801:Humans; D007122:Immunoelectrophoresis; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D002272:Carcinoembryonic Antigen