Comparative studies between in vitro and in vivo effects of human beta-interferon on natural killer activity and its relevance to immunochemotherapy.
A good correlation was found between in vivo and in vitro responses of peripheral MNC from breast cancer patients and the NK boosting effect of human beta IFN. In vitro immunochemistry studies showed that marked antitumor effects were obtained against cultured cancer cells when a widely used chemotherapeutic agent such as 5-FU was combined with nonsensitized spontaneously cytolytic MNC, preactivated in vitro with beta IFN. These results suggest that the in vitro susceptibility assay of MNC to IFNs could be used for predicting favorable responses to immunochemotherapy regimens employing IFNs as immunomodulating agents.
D'Atri
S
S
Institute of Experimental Medicine, National Council of Research (CNR), Rome, Italy.
Fuggetta
M P
MP
Giganti
G
G
Tentori
L
L
Roselli
M
M
Nunziata
C
C
Pastore
S
S
Bonmassar
E
E
De Vecchis
L
L
D003602:Cytotoxicity, Immunologic; D004357:Drug Synergism; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D007167:Immunotherapy; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology* / Q000627:therapeutic use; D007694:Killer Cells, Natural / Q000187:drug effects*; D009369:Neoplasms / Q000628:therapy*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D007370:Interferon Type I; D005472:Fluorouracil