The growth of ordered two-dimensional sheets of ribosomal particles from salt-alcohol mixtures.
A procedure for the in vitro growth of well-ordered two-dimensional sheets from ribosomal particles using salts and salt-alcohol mixtures has been developed. Employing this procedure, ordered two-dimensional sheets of the wild type as well as of mutated 50 S ribosomal subunits from Bacillus stearothermophilus can readily be obtained. These sheets, stained with uranyl acetate or gold-thioglucose, are suitable for three-dimensional image reconstruction. They consist of relatively small unit cells with dimensions of 160 +/- 15 and 365 +/- 20 A. Diffraction patterns of electron micrographs of these sheets contain features to 25 A resolution.
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.
T
T
Arad
J
J
Piefke
H S
HS
Gewitz
B
B
Romberg
C
C
Glotz
J
J
Müssig
A
A
Yonath
H G
HG
Wittmann
D003460:Crystallization; D001411:Geobacillus stearothermophilus; D012270:Ribosomes / Q000648:ultrastructure*