Microtiter assay for acetylcholinesterase.
A microtiter plate adaptation of the classical Ellman colorimetric procedure for measurement of acetylcholinesterase activity is described. This method permits use of an enzyme-linked immunosorbent assay plate reader for rapid analysis of multiple samples and is particularly suitable for analysis of acetylcholinesterase activity on sucrose gradients. The novel procedure is rapid and sensitive and does not require use of radioactive material.
Doctor
B P
BP
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Toker
L
L
Roth
E
E
Silman
I
I
D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D002499:Centrifugation, Density Gradient; D004797:Enzyme-Linked Immunosorbent Assay; D013395:Sucrose
D013395:Sucrose; D000110:Acetylcholinesterase