Time sequence of degeneration pattern of the organ of Corti after acoustic overstimulation. A light microscopical and electrophysiological investigation in the guinea pig.
In order to obtain information about the sequence of events leading to degenerative changes in the cochlea after acoustic trauma, a systematic LM study was performed in the guinea pig organ of Corti. The results were correlated with measurements of CAP N1 thresholds from the same animals. Twenty pigmented guinea pigs were used in the study. Ten of these animals were exposed to a 3.85 kHz pure tone with an intensity varying between 108 and 120 dB SPL for 22.5 to 360 min and were sacrificed after 4 weeks. Eight animals were exposed to 120 dB SPL for 22.5 min and sacrificed at regular intervals after exposure, viz. 5 min, 4, 24 h and 5 days. Prior to sound exposure the animals in the 4-week group were implanted with a permanent round window electrode for measurements of CAP N1 thresholds. The study indicates that the degeneration and reparative processes are not entirely completed after 4 weeks. The development of holes in the reticular lamina may result in further degeneration of cells bilateral to the initial maximum damage. Darkly stained cells seem to be activated in the reparative process in the organ of Corti. Local appearance of dendritic macrophages in the organ of Corti indicate the existence of a local mechanism for disposal of degeneration products from the organ of Corti. The retrograde degeneration of radial nerve fibres seems to be closely associated with that of the pillar cells. A close correlation between structural hair cell damage and CAP N1 threshold shift was found in the region of the organ of Corti corresponding to the exposure frequency. The TS 5 min, 4 h and 24 h after exposure showed a close correlation with swellings in the afferent nerve endings below the IHC in the area of maximum damage and basalwards.
Department of Otolaryngology, Karolinska Institutet, Karolinska sjukhuset, Stockholm, Sweden.
L
L
Fredelius
H
H
Rask-Andersen
B
B
Johansson
R
R
Urquiza
D
D
Bagger-Sjöbäck
J
J
Wersäll
D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D001309:Auditory Threshold; D004586:Electrophoresis; D005260:Female; D006168:Guinea Pigs; D009410:Nerve Degeneration; D009622:Noise; D009925:Organ of Corti / Q000502:physiology*; D013997:Time Factors