Analysis of epidermal proteins for DNA-binding activity.
A group of DNA-binding proteins from the soluble extract of newborn rat epidermis have been separated by chromatography using DNA-cellulose columns. The electrophoretogram of the DNA-binding proteins eluted from a single stranded DNA-cellulose column shows five major proteins of molecular weights ranging between 25K to 40K. Both the epidermal protein filaggrin and most keratins, except two high molecular weight keratins, do not show in vitro DNA-binding activity.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
P C
PC
Supakar
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R
Misra
G M
GM
Bhatnagar
D000818:Animals; D002845:Chromatography; D003101:Collodion; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D051381:Rats; D012867:Skin / Q000032:analysis*
D004268:DNA-Binding Proteins; D003101:Collodion