Adaptative features of enzymes from family Sciaenidae--III. Studies on lactate dehydrogenase (LDH) of fishes from the south coast of Uruguay.
1. Electrophoretic analyses of lactate dehydrogenase isozyme patterns of three species of temperate fish belonging to family Sciaenidae (order Perciformes) indicates that at least three LDH loci--Ldh-A, Ldh-B and Ldh-C are active. 2. The subunits encoded by these loci occurred at different levels in the different tissues and organs analyzed. 3. The products of these loci show different response to changes in temperature. The results were obtained by electrophoretic analyses. 4. Thermostability of skeletal muscle, heart and eye lactate dehydrogenase incubated at 60 degrees C for different periods of time were examined. 5. Relative activities of LDH isozymes were compared by Klebe's method to determine pattern of divergence of duplicated gene expression in the three species studied.
Departmento de Ciências Biológicas, Universidade Federal de São, São Paulo, Brasil.
Z L
ZL
Coppes
M L
ML
Schwantes
A R
AR
Schwantes
D000064:Acclimatization; D000818:Animals; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity; D010473:Perciformes / Q000378:metabolism*; D013045:Species Specificity; D013696:Temperature
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase