Observation of inositol pentakis- and hexakis-phosphates in mammalian tissues by 31P NMR.
In analyzing the 31P NMR spectra of extracts of mammalian tissues and cells we have identified inositol pentakis- and hexakis-phosphates in essentially all of the samples examined. These compounds were present at concentrations of at least 5-15 microM. While the sources and functions of these compounds in mammalian cells are not clear, they may play an important role in phosphoinositol metabolism. For example, one obvious possibility is that these compounds may be sources of or sinks for the Ca++ mobilizing inositol tris- and tetrakis-phosphates.
Nuclear Magnetic Resonance Laboratory, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
B S
BS
Szwergold
R A
RA
Graham
T R
TR
Brown
D000818:Animals; D002417:Cattle; D002460:Cell Line; D005260:Female; D005333:Fetus / Q000032:analysis; D007295:Inositol Phosphates / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000032:analysis; D009374:Neoplasms, Experimental / Q000032:analysis; D010761:Phosphorus Radioisotopes; D010833:Phytic Acid / Q000032:analysis*; D011247:Pregnancy; D051381:Rats; D011915:Rats, Inbred BUF; D013403:Sugar Phosphates / Q000032:analysis*; D014781:Viscera / Q000032:analysis
D007295:Inositol Phosphates; D010761:Phosphorus Radioisotopes; D013403:Sugar Phosphates; C013571:inositol pentaphosphate; D010833:Phytic Acid