Reactions of monoclonal antibodies to human MN blood groups with red blood cells of subhuman primates.
All 12 samples of chimpanzee erythrocytes tested were agglutinated by the commercial polyclonal anti-M reagent of rabbit origin, but none was agglutinated by monoclonal anti-M reagent of murine origin. This, as well as results of absorption experiments, showed that the polyclonal and monoclonal anti-M reagents detect different M epitopes. Polyclonal anti-N reagent of rabbit origin and monoclonal reagent of murine origin obtained by immunization with N blood group substance did not react with any chimpanzee erythrocyte samples. On the other hand, monoclonal anti-N reagent originating from immunization with M blood group substance agglutinated two of the 12 tested chimpanzee erythrocyte samples. This and other experimental results strongly suggested that the latter monoclonal anti-N reagent combined with "N" antigen, i.e., an antigen present on glycophorin B of human M as well as N erythrocytes; apparently erythrocytes of some chimpanzees contain also this antigen.
Department of Microbiology, School of Medicine State University of New York at Buffalo 14214.
R
R
Rubocki
F
F
Milgrom
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D008951:MNSs Blood-Group System / Q000276:immunology*; D002679:Pan troglodytes / Q000097:blood / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D008951:MNSs Blood-Group System