Enzyme-linked immunosorbent assay for serum amyloid A apolipoprotein with use of specific antibodies against synthetic peptides.
We describe a noncompetitive enzyme-linked immunosorbent assay for amyloid A apolipoprotein in human serum (apo SAA) in which specific antibodies against synthetic peptides are used. Microtiter plates were used as solid phase and coated with affinity-purified antibodies raised against SAA1-(95-104) peptide. After incubation with delipidated plasmas, the bound apo SAA was revealed by labeled antibodies raised against SAA1-(58-69) peptide. The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, and non-use of radioisotopes. Results correlate well with those by a nephelometric method in which polyclonal antibodies are used.
Serlia, Institut Pasteur de Lille, France.
R
R
Saïle
C
C
Delpierre
P
P
Puchois
G
G
Hocke
C
C
Cachera
J C
JC
Gesquiere
A
A
Steinmetz
A
A
Tartar
J C
JC
Fruchart
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D001053:Apolipoproteins / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011786:Quality Control; D013223:Statistics as Topic
D000906:Antibodies; D001053:Apolipoproteins; D010446:Peptide Fragments; C035165:apolipoprotein SAA