A simple microassay for the determination of hydrazine in biological samples. Effect of hydrazine and isoniazid on liver and brain glutathione.
A simple microassay for the determination of hydrazine in laboratory samples is presented. The colored product of the reaction of p-dimethylaminobenzaldehyde with hydrazine was tested at 470 nm using double-beam mode in different samples. Internal standards and data on blood serum, liver, and brain of rats treated with hydrazine or isoniazid are presented. The tissue glutathione content of these rats was determined, and the possible implication of glutathione in the brain toxicity of hydrazine is discussed.
Department of Physiology, Faculty of Medicine, University of Valencia, Spain.
T
T
Alvarez de Laviada
F J
FJ
Romero
V
V
Antón
V
V
Antón
J
J
Romá
J
J
Viña
G T
GT
Sáez
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism*; D006834:Hydrazines / Q000032:analysis* / Q000494:pharmacology; D007538:Isoniazid / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats
D006834:Hydrazines; D005978:Glutathione; D007538:Isoniazid