Isolation of angiotensin-converting enzyme inhibitor from tuna muscle.
A novel inhibitor of angiotensin-converting enzyme (ACE) has been discovered and isolated in a pure form from acid extract of tuna muscle by successive column chromatographies and HPLC. The final preparation showed IC50 values of 1 microM and 2 microM for ACEs from bovine and rabbit lungs, respectively. The amino acid sequence of the inhibitor has been established as Pro-Thr-His-Ile-Lys-Trp-Gly-Asp by the Edman procedure and carboxypeptidase digestion.
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Y
Y
Kohama
S
S
Matsumoto
H
H
Oka
T
T
Teramoto
M
M
Okabe
T
T
Mimura
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis*; D009842:Oligopeptides / Q000302:isolation & purification; D011817:Rabbits; D014413:Tuna
D000806:Angiotensin-Converting Enzyme Inhibitors; D009842:Oligopeptides; C062708:tuna AI