Conserved amino acid residues arginine 33 and tyrosine 123 are critical for the antiviral activity of murine interferon-alpha 1.
Analogues of murine interferon-alpha 1 were produced by site directed mutagenesis and expressed in vitro. Replacement of the conserved residue arginine 33 by glutamic acid resulted in an interferon with no detectable antiviral activity. Substitution of tyrosine residue 123 shows dramatic reductions in the antiviral activity. These analogues demonstrate the importance of the conserved residues arginine 33 and tyrosine 123 in maintaining the antiviral activity of murine interferon-alpha 1.
Centre For Molecular Biology and Medicine, Monash University, Clayton, Victoria, Australia.
J A
JA
Kerry
M J
MJ
Tymms
A W
AW
Linnane
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001120:Arginine / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007370:Interferon Type I / Q000494:pharmacology*; D051379:Mice; D014443:Tyrosine / Q000032:analysis*
D000998:Antiviral Agents; D007370:Interferon Type I; D014443:Tyrosine; D001120:Arginine