Purification and characterization of a lectin from Phaseolus acutifolius var. latifolius.
The lectin from Phaseolus acutifolius var. latifolius has been purified to homogeneity by affinity chromatography using stroma-sephadex, gel filtration and electrofocusing. The purified lectin consists of four subunits of 21 Kd molecular mass each one with 7.4% w/w total carbohydrate. Red cells of human and animal species, are agglutinated but are not inhibited by a series of sugars. Mitogenicity and immunosuppressive activities were demonstrated. Agglutination requires magnesium and calcium ions.
Unidad de Medicina Experimental, Facultad de Medicina, C.U., Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
F
F
Vargas-Albores
G
G
de la Fuente
C
C
Agundis
F
F
Córdoba
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002845:Chromatography; D007887:Fabaceae / Q000032:analysis*; D006801:Humans; D007166:Immunosuppressive Agents; D066298:In Vitro Techniques; D007526:Isoelectric Point; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008970:Molecular Weight; D010835:Phytohemagglutinins / Q000302:isolation & purification* / Q000494:pharmacology; D037121:Plant Lectins; D010946:Plants, Medicinal; D013696:Temperature
D000596:Amino Acids; D007166:Immunosuppressive Agents; D037102:Lectins; D010835:Phytohemagglutinins; D037121:Plant Lectins