Evaluation by immunoblotting of the criteria for tubular lesions diagnostic with urinary SDS-PAGE.
Immunoblotting methods have allowed the identification of 14 urinary proteins. From this study, it appears that some low-relative-molecular mass proteins are derived from disrupted high-relative-molecular mass proteins. The only reliable bands for the diagnosis of tubular lesions, on the polyacrylamide gel stained with Coomassie Blue, are retinol-binding-protein and beta 2-microglobulin. alpha 1-microglobulin can overlap with breakdown products of albumin. The visualization of Ig light chains and lysozyme is rather poor after Coomassie Blue stainings and the accurate identification of these bands may be only improved by the use of immunoblotting.
Unité des Protéines No. 16, Institut National de la Santé et de la Recherche Médicale (INSERM), Lille, France.
J M
JM
Perini
B
B
Dehon
T
T
Marianne
M
M
Foulard
P
P
Roussel
D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000032:analysis; D007674:Kidney Diseases / Q000652:urine*; D007684:Kidney Tubules / Q000378:metabolism; D009961:Orosomucoid / Q000652:urine; D011507:Proteinuria / Q000652:urine*; D012967:Sodium Dodecyl Sulfate
D007074:Immunoglobulin G; D009961:Orosomucoid; D012967:Sodium Dodecyl Sulfate; D003101:Collodion