Sensitive HPLC assay for thiopental in human serum after simple preparation of the samples. Its application for clinical research.
A simple, precise and sensitive micro method for the determination of thiopental in human serum is presented. After deproteinization with acetonitrile the supernatant was directly injected into a reversed phase HPLC system with UV photometrical detection at 280 nm. The limit for the detection of thiopental was less than 0.1 microgram/ml serum. Time consuming extraction of the serum sample was not necessary. The method can be recommended for clinical routine analysis; its suitability has been demonstrated in several studies.
Institut für Anaesthesie, Universität des Saarlandes, Homburg/Saar, FRG.
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Altmayer
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Büch
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Büch
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D012709:Serum Albumin / Q000378:metabolism; D013874:Thiopental / Q000097:blood* / Q000493:pharmacokinetics
D012709:Serum Albumin; D013874:Thiopental