Identification of the human beta-actin enhancer and its binding factor.
An enhancer of the human beta-actin gene and a factor that specifically interacts with it were detected. A mobility shift assay showed that the factor bound to the 25-base-pair sequence (between +759 and +783 downstream from the cap site) with high specificity. This finding correlated with those of DNase I protection and exonuclease III digestion assays. This binding region of the beta-actin enhancer contained a hyphenated dyad symmetry and an enhancer core-like sequence. In vitro competition experiments indicated that the factor did not bind to the simian virus 40 enhancer core region.
Department of Oncogene Research, Osaka University, Japan.
T
T
Kawamoto
K
K
Makino
H
H
Niwa
H
H
Sugiyama
S
S
Kimura
M
M
Amemura
A
A
Nakata
T
T
Kakunaga
D000123:Acetyltransferases / Q000235:genetics; D000199:Actins / Q000235:genetics*; D001665:Binding Sites; D015500:Chloramphenicol O-Acetyltransferase; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D005090:Exodeoxyribonucleases; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*
D000199:Actins; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D003850:Deoxyribonuclease I