[Model substrates of enzymes modifying ribonucleic acids. Synthesis of deca- and undecanucleotid-fragments of 21-member oligoribonucleotide simulating T psi C-branch of yeast valine tRNA].
Decanucleotide (Ap)6GpTpUpC and undecanucleotide GpApUpCpCp (Up)5U have been synthesised. They constitute 5'- and 3'-parts of a 21-mer which imitates T psi C-arm of yeast tRNA(Val1) and is a potential substrate for m1A-methylases and pseudouridine synthetase. The oligonucleotide blocks, synthesised enzymatically by means of ribonucleases of various substrate specificity and polynucleotide phosphorylases (TpUpC, ApUpCpC, pGpTpUpC, GpApUpCpC) or obtained by hydrolysis of poly(U) and poly(A) with Serratia marcescens endonuclease (hexauridilate and hexaadenilate), were joined by T4 RNA ligase.
Khabarova
M I
MI
Zhenodarova
S M
SM
D019751:Intramolecular Transferases; D007535:Isomerases / Q000378:metabolism; D009843:Oligoribonucleotides / Q000096:biosynthesis* / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012366:RNA, Transfer, Val / Q000378:metabolism*; D013379:Substrate Specificity; D015003:Yeasts / Q000378:metabolism; D012359:tRNA Methyltransferases / Q000378:metabolism*
D009843:Oligoribonucleotides; D012331:RNA, Fungal; D012345:RNA, Transfer, Amino Acid-Specific; D012366:RNA, Transfer, Val; D012359:tRNA Methyltransferases; C023194:tRNA (adenine(58)-N(1))-methyltransferase; D007535:Isomerases; D019751:Intramolecular Transferases; C023199:tRNA-pseudouridine synthase I
Model'nye substraty fermentov modifikatsii ribonukleinovykh kislot. Sintez deka- i undekanukleotida-fragmentov 21-chlennogo oligoribonukleotida, modeliruiushchego T psi C-vetv' drozhzhevoĭ valinovoĭ tRNK.