Promoter occlusion during ribosomal RNA transcription.
The ability of RNA polymerase I to read through a promoter-bound transcription initiation factor (TIF) was assessed using a dimeric ribosomal RNA gene promoter. Transcription from the upstream promoter is unaffected by TIF bound to the downstream promoter; RNA polymerase I is freely able to read through DNA-bound TIF. In contrast, transcription from the downstream promoter is inhibited by the passage of RNA polymerase I. Readthrough by RNA polymerase I disrupts the downstream TIF-DNA complex, and alters the TIF footprint. A general function for sequences leading to transcription termination upstream of rRNA or other promoters may be the prevention of promoter occlusion in tandem arrays of genes.
Bateman
E
E
Colorado State University, Department of Biochemistry, Fort Collins 80523.
Paule
M R
MR
D000048:Acanthamoeba; D000818:Animals; D004268:DNA-Binding Proteins / Q000502:physiology*; D003850:Deoxyribonuclease I; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000502:physiology*; D012335:RNA, Ribosomal / Q000235:genetics; D013698:Templates, Genetic; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D012335:RNA, Ribosomal; D014157:Transcription Factors; D012318:RNA Polymerase I; D003850:Deoxyribonuclease I