All six rat gamma-crystallin genes are located on chromosome 9.
The rat genome contains six genes for the lens-specific gamma-crystallins. Five of these genes are clustered; the location of the sixth gene is not known. To determine whether the sixth gene is located on the same chromosome as the gamma-crystallin gene cluster, a panel of mouse-rat hybrid cell lines, segregating rat chromosomes, was screened for the presence of gamma-crystallin sequences. We show here that the hybrid cell lines contain either all rat gamma-crystallin genes or none, indicating that all six genes map to the same chromosome. The presence of rat gamma-crystallin sequences in the hybrid cell lines was concordant only with the presence of rat chromosome 9, which therefore contains the rat gamma-crystallin genes.
Den Dunnen
J T
JT
Laboratory of Molecular Biology, University of Nijmegen, The Netherlands.
Szpirer
J
J
Levan
G
G
Islam
Q
Q
Schoenmakers
J G
JG
D000818:Animals; D002874:Chromosome Mapping; D003459:Crystallins / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D006822:Hybrid Cells; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D051381:Rats
D003459:Crystallins; D004247:DNA