Efficient synthesis of N alpha-acetylarginine methylamide.
A simple preparation of Ac-Arg-(p-TosH or HCl)-NHMe is described. The NG-protonated Z-Arg was coupled with methylamine by the mixed anhydride method. Z-Arg-NHMe was purified as a NG-p-toluene sulfonate salt by crystallization from water. After removal of the Z group by catalytic hydrogenation and acetylation Ac-Arg(p-TosH)-NHMe was obtained. Ac-Arg(HCl)-NHMe was prepared by chromatography of the NG-TosH derivative on Dowex 44 (in Cl- form).
Deutsches Wollforschungsinstitut an der RWTH Aachen.
J
J
Wiese
V K
VK
Naithani
H
H
Zahn
D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D006868:Hydrolysis; D014357:Trypsin
C055074:N(alpha)-acetylarginine methylamide; D001120:Arginine; D014357:Trypsin