The effect of combined treatment of HeLa cells with actinomycin D and radiation upon survival and recovery from radiation damage.
The presence of actinomycin D during irradiation of HeLa cells leads to radiosensitization under aerobic but not under hypoxic conditions. Depending on the time interval between incubation with the drug and irradiation, pre- or post-treatment with actinomycin D also increases the radiosensitivity of HeLa cells irradiation in air. Pretreatment with actinomycin D does not inhibit recovery from potentially lethal radiation damage. In split-dose experiments, recovery from sublethal radiation damage was not impaired by the drug if present during the entire fractionation interval or if it was given as a short exposure after the first radiation dose.
Strahlenbiologisches Institut der Ludwig-Maximilians-Universität München, F.R.G.
W
W
Ziegler
P
P
Birkenfeld
K R
KR
Trott
D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D003131:Combined Modality Therapy; D003609:Dactinomycin / Q000008:administration & dosage / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D011829:Radiation Dosage; D011836:Radiation Tolerance; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage / Q000494:pharmacology*
D011838:Radiation-Sensitizing Agents; D003609:Dactinomycin