Ethanol-induced accumulation of triacylglycerol in cultured hepatocytes: dependency on ethanol metabolism.
Calculations based on net changes and [1-14C]oleate incorporation data suggest that fatty acids are the only precursor of acetylCoA and that extensive lipolysis takes place in hepatocyte cultures incubated with 1 mM oleate in the absence or presence of ethanol. The effect of 4-methylpyrazole on fatty acid metabolism as affected by ethanol has been studied. The ethanol-induced increase in fatty acid uptake was found to be an effect of ethanol per se, whereas the decreased oxidation of fatty acids and secretion of VLDL-triacylglycerol was found to be due to ethanol metabolism.
Department of Biochemistry A, Panum Institute, University of Copenhagen, Denmark.
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Grunnet
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Kondrup
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Dich
D000818:Animals; D002478:Cells, Cultured; D000431:Ethanol / Q000493:pharmacokinetics / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D000077604:Fomepizole; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism*
D005227:Fatty Acids; D009829:Oleic Acids; D011720:Pyrazoles; D014280:Triglycerides; D019301:Oleic Acid; D000431:Ethanol; D000077604:Fomepizole