Elastolysis of normal and partially cross-linked elastin.
Rate of elastolysis by pancreatic and leukocyte elastases of normal and copper deficient porcine aortic elastins were measured using a conductimetric method. Kinetics obey to Michaelis-Menten model for both substrates and enzymes. KM and Vmax values derived from Lineweaver-Burk plots indicate that, if a near uniformity exists in KM, differences were observed in catalytic rates, kcat increasing approximately 40% for copper deficient elastin elastolysis by leukocyte elastase. This higher susceptibility to proteolysis may have implications for understanding turnover of elastin in tissues.
Bostancioglu
K
K
UA CNRS 244, I.C.B.M.C., Université Lyon I, Villeurbanne, France.
Mecham
R P
RP
Wallach
J M
JM
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D003300:Copper / Q000172:deficiency; D004549:Elastin / Q000378:metabolism*; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism*; D013552:Swine
D003300:Copper; D004549:Elastin; D010196:Pancreatic Elastase