Value of a new screening test for respiratory allergy.
Phadiatop (Pharmacia) is a new in vitro screening test for respiratory allergy which detects in serum the presence of specific IgE to a mixture of common inhalant allergens; results are either positive or negative. Among 100 patients suffering from rhinitis and/or asthma, investigated with 15 skin prick tests (SPT) and RAST for frequent allergens, the Phadiatop was positive in 63: all 63 had at least one positive SPT and RAST (specificity: 100%). Conversely, ther were 67 patients who had a positive RAST for at least one allergen: among these 67 the total IgE concentration in serum was high (greater than 100 kU/l) in 43 (64%) and the Phadiatop was positive in 63 (94%). Phadiatop was negative in serum obtained from 10 cord bloods and 10 non-allergic subjects (disease-free, 15 negative SPT and RAST). Results of the radio-immunoassay originally proposed by the manufacturer were similar to those obtained with a modified enzymo assay using a fluorometric substrate. In the prediction of respiratory allergy, Phadiatop was found to be specific and more accurate than the determination of total IgE in serum.
Duc
J
J
Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Peitrequin
R
R
Pécoud
A
A
D000293:Adolescent; D000328:Adult; D000485:Allergens; D001249:Asthma / Q000175:diagnosis; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis*; D007073:Immunoglobulin E / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011852:Radioallergosorbent Test / Q000379:methods*; D011863:Radioimmunoassay / Q000379:methods*; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis
D000485:Allergens; D007073:Immunoglobulin E