The primary structure of the hemoglobins of the adult jaguar (Panthera onco, Carnivora).
The primary structure of the hemoglobins from Jaguar (Panthera onco) are presented. Electrophoretic separations without and with a dissociating agent revealed the presence of two hemoglobin components, alpha 2 beta I2 and alpha 2 beta II2. The separation of the hemoglobin components was achieved by ion-exchange chromatography. The globin chains were separated by ion-exchange chromatography and also by reversed phase HPLC. The amino-acid sequences of the native chains and peptides were determined by liquid-phase and gas-phase sequencing. N-Acetylserine was detected by FAB-mass spectroscopy as N-terminal group of the beta I chain. The sequences are compared with that of human hemoglobin (Hb A).
Max-Planck-Institut für Biochemie, Abt. Proteinchemie, Martinsried bei München.
A
A
Ahmed
M
M
Jahan
Z H
ZH
Zaidi
G
G
Braunitzer
D000595:Amino Acid Sequence; D000818:Animals; D002335:Carnivora / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006454:Hemoglobins / Q000032:analysis*; D010084:Oxidation-Reduction
D006454:Hemoglobins