A Mycoplasma gallisepticum strain-specific DNA probe.
Total DNA from the vaccine F strain (K810) and the reference S6-strain of Mycoplasma gallisepticum (MG) was cloned in Escherichia coli using the plasmid pUC8. A 6-kilobase fragment, specific for the vaccine strain, was identified by colony dot and Southern hybridization analyses. When labeled and used as a probe, this fragment hybridized with the homologous and one other vaccine F-strain (F2F10), but it did not hybridize with other MG strains (Fg38, S6, A5969, V503) or with three other species of avian mycoplasmas.
Khan
M I
MI
Department of Epidemiology & Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
Kirkpatrick
B C
BC
Yamamoto
R
R
D001428:Bacterial Vaccines / Q000032:analysis*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D009174:Mycoplasma / Q000276:immunology*; D013045:Species Specificity
D001428:Bacterial Vaccines; D004269:DNA, Bacterial