Transepithelial migration of eosinophils in experimental nasal allergy in guinea pigs.
We examined eosinophil-migration through the nasal epithelium into nasal cavity in a guinea pig model exhibiting 8-day passive cutaneous anaphylaxis antibody-dependent nasal allergy. Transmission electron microscopic observation of this process revealed that eosinophils traversed the epithelium through the intercellular space and split the tight-junctions of epithelial lining cells. Freeze-fracture studies of this process showed that the morphology of tight-junction was not changed after eosinophil-migration. These observations may indicate that tight-junctions close after eosinophil-migration.
Samejima
Y
Y
Department of Otolaryngology, Kumamoto University, School of Medicine, Japan.
Masuyama
K
K
Ishikawa
T
T
D000818:Animals; D002465:Cell Movement; D004804:Eosinophils / Q000502:physiology* / Q000648:ultrastructure; D004848:Epithelium / Q000276:immunology / Q000648:ultrastructure; D005260:Female; D005614:Freeze Fracturing; D006168:Guinea Pigs; D007365:Intercellular Junctions / Q000648:ultrastructure; D009297:Nasal Mucosa / Q000276:immunology* / Q000648:ultrastructure; D009299:Nasal Provocation Tests; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000473:pathology