Bovine antibody dependent cell-mediated cytotoxicity (ADCC) effector cells.
ADCC effector cells from bovine blood were separated by centrifugation, adherence and rosetting techniques. Each enriched cell population, peripheral blood mononuclear cells (PBM), null lymphocytes, monocytes and neutrophils, was then examined for its capacity to mediate ADCC. Utilizing heterologous sensitizing antisera it was found that monocytes had approximately twice the ADCC activity of null lymphocytes and that neutrophils had essentially no activity. However, when homologous sensitizing antisera were used it was found that neutrophils possessed the greatest activity followed by monocytes and null cells. Results confirm the existence of an ADCC active null lymphocyte in the bovine.
Belden
E L
EL
Department of Molecular Biology, College of Agriculture, University of Wyoming, Laramie 82071.
Peng
H M
HM
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002417:Cattle / Q000276:immunology*; D002448:Cell Adhesion; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006825:Hybridomas; D007106:Immune Sera / Q000276:immunology; D008215:Lymphocytes, Null / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D012397:Rosette Formation
D007106:Immune Sera