Cloning and expression of the metallo-proteinase inhibitor (S-MPI) gene from Streptomyces nigrescens.
The coding and regulatory regions of a proteinaceous metallo-proteinase inhibitor (S-MPI) gene have been cloned from the genomic DNA of Streptomyces nigrescens using a deoxyinosine-containing synthetic probe designed from the amino acid sequence of the S-MPI protein. The S-MPI gene was located to the DNA region of about 1.2 kilobase pairs, which was verified to be sufficient for the gene expression in S. lividans.
Tanaka
K
K
Institute of Applied Microbiology, University of Tokyo, Japan.
Saito
H
H
Oda
K
K
Murao
S
S
Takahashi
H
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D007700:Kinetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D011480:Protease Inhibitors; C056999:metalloproteinase inhibitor, Streptomyces; D008666:Metalloendopeptidases