[A method for detecting protein-protein interactions. Detection of proteins with the molecular weight of approximately 37 kD binding with tryptophanyl-tRNA-synthetase].
A procedure for detecting protein-protein interactions is proposed. It is based on blotting of electrophoretically separated protein mixtures followed by detection of the proteins interacting with a given 125I-labelled protein used as a probe. Application of this approach to lysates of cultured mammalian cells enabled us to reveal a unique 37 kDa polypeptide interacting with 125I-labelled bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2).
Beresten'
S F
SF
Rubikaĭte
B I
BI
Kiselev
L L
LL
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism; D014369:Tryptophan-tRNA Ligase / Q000378:metabolism*
D046911:Macromolecular Substances; D010455:Peptides; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase
Metod vyiavleniia belok-belkovykh vzaimodeĭstviĭ. Obnaruzhenie belkas M 37 kDa, sviazyvaiushchegosia s triptofanil-tRNK-sintetazoĭ.