[An experimental study on the effect of fluorocarbon perfusion for the preservation of an amputated limb].
We assessed the ability of oxygenated fluorocarbon (chilled Fluosol-DA) to minimize the effect of ischemia on devascularized skeletal muscle of dog. 1) Short term follow-up study: Perfusion of the limb with Fluosol-DA solution significantly minimized edema formation and leakage of skeletal muscle enzymes (GOT, CPK) into the serum 5.5 hours after revascularization as compared to Collins sol., lactated Ringer's sol. or merely cooling. Histological finding showed that the Fluosol-DA group had significantly less hyaline degeneration and separation of the muscle fiber from the endomysium than the groups receiving Collins sol., lactated Ringer's sol. or merely cooling group. 2) Long term follow-up study: The Fluosol-DA group showed less enzyme elevation than the Collins sol. and lactated Ringer's sol. groups. Histologically, the Collins sol. and lactated Ringer's sol. group showed significant scarring compared to the Fluosol-DA group. It was concluded that perfusion with chilled Fluosol-DA was effective for preservation of devascularized skeletal muscle.
Department of Orthopedic Surgery, Jikei University, School of Medicine, Japan.
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Takahashi
D000671:Amputation, Surgical; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D004285:Dogs; D004338:Drug Combinations; D005069:Evaluation Studies as Topic; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D009132:Muscles / Q000201:enzymology / Q000473:pathology*; D010477:Perfusion; D012092:Replantation; D000077325:Ringer's Lactate; D014021:Tissue Preservation / Q000379:methods*
C024102:Collins' solution; D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase