Human breast-milk factors influencing lipid metabolism by fetal rabbit aorta in organ culture.
Effects of human or rabbit milk on cholesterol content, incorporation of [14C]oleate, and DNA synthesis were investigated in organ cultures of aorta from fetal and suckling rabbits. Human skim milk (50 mL/L) in organ culture decreased content (mumol/g protein) of aortic total cholesterol (control: 162.9 +/- 24.6 milk: 117.6 +/- 4.9) with significant decrease in cholesteryl esters (control: 16.5 +/- 5.1, milk: 2.3 +/- 0.5). The effect was observed in lipoprotein (total cholesterol: 120.2 +/- 8.8) and lipoprotein-free (total cholesterol: 85.4 +/- 5.1) fractions with molecular weight greater than 100,000. The human milk fraction with molecular weight greater than 100,000 was also the most active in promoting [3H]thymidine incorporation into DNA in fetal aorta. Although milk stimulated the incorporation of [14C] oleate into triglycerides (control: 38.8 +/- 2.5%, milk: 82.1 +/- 4.2%), decreased incorporation to phospholipids (control: 55.0 +/- 3.0%, milk: 11.3 +/- 1.8%) was observed. These studies suggest that milk contains factors influencing aortic lipid metabolism during development.
Park
M S
MS
Department of Internal Medicine, University of Cincinnati, OH.
Subbiah
M T
MT
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000032:analysis; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008892:Milk; D008894:Milk Proteins / Q000494:pharmacology; D008895:Milk, Human; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D009924:Organ Culture Techniques; D011817:Rabbits; D013936:Thymidine / Q000378:metabolism
D002788:Cholesterol Esters; D008894:Milk Proteins; D009829:Oleic Acids; D019301:Oleic Acid; D004247:DNA; D002784:Cholesterol; D013936:Thymidine