Osmotic water permeability in glycoprotein containing liposomes.
The kinetics of osmotic water permeability in proteoliposomes containing alpha 1-acid glycoprotein was investigated by means of stopped-flow spectrophotometry. A biphasic time-course of scattered light with time was registered. The rate constants calculated from fits to an exponential function in the first phase were proportional to the final medium osmolarity. The apparent second order rate constants Kapp (Osm-1 sec-1) were determined at different glycoprotein concentrations in the original mixture for preparation of proteoliposomes. The value of Kapp at lipid:glycoprotein weight ratio = 1 was plotted in Arrhenius coordinates. The calculated activation energy for water permeation through the lipid bilayer suggests that eventual channel mechanism may be involved due to the presence of glycoprotein molecule in the liposomes.
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
V Z
VZ
Neitchev
A P
AP
Kostadinov
D006982:Hypertonic Solutions; D008081:Liposomes / Q000378:metabolism*; D009961:Orosomucoid; D009995:Osmosis; D010539:Permeability; D014867:Water / Q000378:metabolism*
D006982:Hypertonic Solutions; D008081:Liposomes; D009961:Orosomucoid; D014867:Water