Application of fast LC to pharmaceutical analysis: dopamine.HCl.
A reversed-phase HPLC method for the determination of the dopamine hydrochloride content of pharmaceutical intravenous solutions is developed using short (3-cm) columns packed with 3-micron particles. Total analysis time for the stability indicating assay, which is capable of separating the analyte from all its common degradates and formulation matrix components, is 2 min. The assay is equivalent in performance (accuracy, precision, separation behavior, ruggedness) to a standard method recommended by the United States Pharmacopeia (USP). Common components of conventional HPLC systems are evaluated with respect to their utility for this application of fast LC.
Travenol Laboratories, Inc., Morton Grove, IL 60053.
D S
DS
Brown
D R
DR
Jenke
D002853:Chromatography, Liquid; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry; D013056:Spectrophotometry, Ultraviolet
D004298:Dopamine