A comparison of two dye-binding methods for the determination of dog, rat and human plasma albumins.
Plasma albumin was determined in human, rat and dog samples by bromocresol green and bromocresol purple dye-binding methods. The bromocresol purple method produced significantly lower values in dog and rat plasma samples, although the use of homologous standards reduced the differences between the two methods. These observations reflect differing affinities of the two dyes for albumins of different species.
Evans
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GO
Department of Toxicology and Metabolism, Wellcome Research Laboratories, Beckenham, Kent, U.K.
Parsons
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CE
D000818:Animals; D001961:Bromcresol Green; D001962:Bromcresol Purple; D003408:Cresols; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008722:Methods; D009994:Osmolar Concentration; D051381:Rats; D012709:Serum Albumin / Q000032:analysis*
D003408:Cresols; D012709:Serum Albumin; D001962:Bromcresol Purple; D001961:Bromcresol Green