Characterization and quantification of PAF-acether (platelet-activating factor) as a heptafluorobutyrate derivative of 1-O-alkyl-2-acetyl-sn-glycerol by capillary column gas chromatography with electron-capture detection.
A gas chromatographic method with a glass capillary column and electron-capture detection is proposed for the characterization and quantification of 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine (PAF C16) using the corresponding 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-heptafluorobutyrate derivative and, as an internal standard, 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine. The reproducibility was approximately 5% and amounts as low as 20 pg could be measured. The method was specific and allowed the quantification of PAF C16 in supernatants from stimulated human polymorphonuclear neutrophils.
Laboratoire de Chimie Analytique II, S.A.M.M., Faculté de Pharmacie, Chatenay Malabry, France.
M J
MJ
Bossant
R
R
Farinotti
J M
JM
Mencia-Huerta
J
J
Benveniste
G
G
Mahuzier
D002849:Chromatography, Gas; D004563:Electrochemistry; D005466:Fluorocarbons; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D009504:Neutrophils / Q000032:analysis; D010972:Platelet Activating Factor / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis; D014280:Triglycerides / Q000032:analysis
D005466:Fluorocarbons; D007202:Indicators and Reagents; D010972:Platelet Activating Factor; D014280:Triglycerides; C055317:1-O-hexadecyl-2-O-acetyl-sn-glycero-3-heptafluorobutyrate; C033094:perfluorobutyric acid