Macrophage cholesterol removal by triglyceride-phospholipid emulsions.
Phospholipid liposomes were previously shown to mobilize cholesterol from cultured macrophage foam cells. Because Intralipid, a clinically available triglyceride-phospholipid emulsion, contains both phospholipid liposomes and triglyceride-emulsion particles, we sought to study its effect on macrophage cholesterol mobilization. Following an 18h incubation of J774 macrophages in serum-free medium supplemented with Intralipid, cholesteryl ester content decreased by up to 50% in previously cholesterol-loaded cells, and by 25% in non-loaded cells. Both components of Intralipid, liposomes and emulsion particles, independently caused reductions in cellular cholesteryl ester. We conclude that clinically available triglyceride-phospholipid emulsions can mobilize macrophage cholesterol in vitro.
Aviram
M
M
Columbia University, College of Physicians & Surgeons, New York, New York 10032.
Williams
K J
KJ
Deckelbaum
R J
RJ
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D005217:Fat Emulsions, Intravenous; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010743:Phospholipids; D014280:Triglycerides
D004655:Emulsions; D005217:Fat Emulsions, Intravenous; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol