Evidence that the functional beta-actin gene is single copy in most mice and is associated with 5' sequences capable of conferring serum- and cycloheximide-dependent regulation.
Hybridization to synthetic oligonucleotides representing conserved regions in the promoter and first intron of several vertebrate beta-actin genes was used to discriminate between what appears to be a single functional beta-actin gene and numerous pseudogenes in the mouse genome. Sequences derived from the 5' end of this gene were shown to confer serum-inducible expression upon a heterologous reporter gene when transfected into mouse fibroblasts. Moreover, these sequences rendered reporter gene expression superinducible by a combination of serum and cycloheximide. These experiments indicate that the 5' end of the mouse beta-actin gene contains sequence elements which mediate the stimulatory effects of serum growth factors and which are responsive to both positive and negative regulators of gene expression.
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905.
P K
PK
Elder
C L
CL
French
M
M
Subramaniam
L J
LJ
Schmidt
M J
MJ
Getz
D000123:Acetyltransferases / Q000235:genetics; D000199:Actins / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes; D006133:Growth Substances / Q000097:blood / Q000494:pharmacology*; D051379:Mice; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
D000199:Actins; D003470:Culture Media; D006133:Growth Substances; D011993:Recombinant Fusion Proteins; D003513:Cycloheximide; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase