Rat and human liver vitamin K epoxide reductase: inhibition by thiol blockers and vitamin K1.
1. Reduction of vitamin K1 2,3-epoxide by rat and human liver vitamin K epoxide reductase is inhibited by N-ethylmaleimide and iodoacetamide. 2. Both enzymes are protected from inhibition by N-ethylmaleimide by vitamin K1 or vitamin K1 2,3-epoxide. 3. Vitamin K1 inhibits reduction of vitamin K1 2,3-epoxide to vitamin K1 which suggests product inhibition of the enzyme.
Department of Physiology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
R
R
Wallin
S D
SD
Patrick
L F
LF
Martin
D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D010837:Vitamin K 1 / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D064417:Vitamin K Epoxide Reductases
D013438:Sulfhydryl Compounds; C016186:vitamin K1 oxide; D010837:Vitamin K 1; D006899:Mixed Function Oxygenases; D064417:Vitamin K Epoxide Reductases