3-Hydroxy-3-methylglutaryl-CoA reductase, mevalonate-5-pyrophosphate decarboxylase and acyl-CoA: cholesterol acyl-transferase from mucosa scrapings and isolated enterocytes from chick duodenum, jejunum and ileum.
3-Hydroxy-3-methylglutaryl-CoA reductase, mevalonate-5-pyrophosphate decarboxylase and acyl-CoA: cholesterol acyltransferase activities were assayed in mucosal scrapings and isolated enterocytes from chick duodenum, jejunum and ileum. Maximal reductase and decarboxylase specific activities were found in ileum and jejunum, while ileum exhibited the minimal acyltransferase specific activity. The isolated epithelial cells showed levels of reductase and acyltransferase specific activities higher than those found in mucosa scrapings, probably due to the contact of these microsomal proteins with proteolytic enzymes during homogenization of the mucosa. However, no protecting effect of the trypsin inhibitor (2mg/ml) could be observed on reductase activity in mucosa scrapings. The cytosolic location of decarboxylase may account for the similar levels of specific activities found in mucosa scrapings and isolated enterocytes.
Departamento de Bioquímica y Biología Molecular, Universidad de Granada, Spain.
J
J
Iglesias
D
D
González-Pacanowska
E
E
García-Peregrin
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002645:Chickens; D004386:Duodenum / Q000201:enzymology; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D007082:Ileum / Q000201:enzymology; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology*; D007421:Intestine, Small / Q000166:cytology / Q000201:enzymology*; D007583:Jejunum / Q000201:enzymology; D008297:Male; D009928:Organ Specificity; D002785:Sterol O-Acyltransferase / Q000378:metabolism*
D006903:Hydroxymethylglutaryl CoA Reductases; D002785:Sterol O-Acyltransferase; D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase