Cell separation by immunoaffinity partitioning with polyethylene glycol-modified protein A in aqueous polymer two-phase systems.
Previous work has shown that polyethylene glycol (PEG)-bound antibodies can be used as affinity ligands in PEG-dextran two-phase systems to provide selective partitioning of cells to the PEG-rich phase. In the present work we show that immunoaffinity partitioning can be simplified by use of PEG-modified Protein A which complexes with unmodified antibody and cells and shifts their partitioning into the PEG-rich phase, thus eliminating the need to prepare a PEG-modified antibody for each cell type. In addition, we provide a more rigorous test of the original technique with PEG-bound antibodies by showing that it is effective at shifting the partitioning of either cell type of a mixture of two cell populations.
Karr
L J
LJ
Space Science Laboratory, NASA/Marshall Space Flight Center, Huntsville, AL 35812.
Van Alstine
J M
JM
Snyder
R S
RS
Shafer
S G
SG
Harris
J M
JM
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002846:Chromatography, Affinity; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000032:analysis; D011092:Polyethylene Glycols; D013205:Staphylococcal Protein A
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D011092:Polyethylene Glycols