Use of GC/MS/SIM for rapid determination of plasma levels of o,p'-DDD, o,p'-DDE and o,p'-DDA.
A new method for extraction and quantification of plasma o,p'-DDD (2,2-(2-chlorophenyl,4'-chlorophenyl)1,1-dichloroethane) and its metabolites has been developed. When plasma (0.1 ml) adsorbed to and dried on a filter paper was heated with 5% hydrogen chloride in methanol (1 ml) in a boiling water bath, o,p'-DDD and its metabolites were liberated from the serum protein and were able to be easily extracted with benzene. The recovery rate was raised compared with conventional methods. In addition, this procedure also carried out the simultaneous methylation of o,p'-DDA, one of the metabolites. Mass numbers of 199, 210, 235 and 246 were selected from the mass spectra of o,p'-DDD and its related substances, and they were monitored. Identification was performed on the basis of the retention time and the mass peak intensity ratio. Quantitative determination was performed using the internal standard technique. It was learned that o,p'-DDA is present in the plasma at a concentration about 10 times higher than the levels of o,p'-DDD and o,p'-DDE.
Department of Public Health, Kobe University School of Medicine, Japan.
M
M
Inouye
T
T
Mio
K
K
Sumino
D002940:Circadian Rhythm; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008939:Mitotane / Q000031:analogs & derivatives* / Q000097:blood*
C054917:2,2-(2-chlorophenyl-4'-chlorophenyl)acetic acid; D008939:Mitotane; C054919:2,2-(2-chlorophenyl-4'-chlorophenyl)-1,1-dichloroethene