[The isolated perfused rat liver--methodology and viability characteristics].
A simple method for in vitro perfusion of the rat liver is presented. The operative technique for mobilizing the liver, the equipment, the medium, and the conditions used for the perfusion were described in detail. Directions for cleaning and sterilising the system are mediate. The represented perfusion system is suitable not only for the system are recirculatory but also for non-recirculatory organ perfusions. The problems of the viability of the perfused liver are discussed and the different qualification of several viability parameters is debated.
Brömme
H J
HJ
Institut für Biochemie, Martin-Luther-Universität Halle-Wittenberg.
Blech
W
W
D000818:Animals; D002470:Cell Survival; D005260:Female; D005943:Gluconeogenesis; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D010101:Oxygen Consumption; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D007773:Lactates; D011773:Pyruvates
Die isoliert perfundierte Rattenleber--Methodik und Vitalitätscharakteristik.