Immunochemical and biological properties of a mouse monoclonal antibody reactive to prunus necrotic ringspot ilarvirus.
A monoclonal antibody reacting with prunus necrotic ringspot ilarvirus was tested in immunochemical studies, neutralization of infectivity assays, and by immuno-electron microscopy. The antibody was able to detect the 27,000 Mr coat protein of prunus necrotic ringspot ilarvirus in western blots and also detected all polypeptide fragments generated after incubation of whole virus with proteolytic enzymes. In neutralization of infectivity studies, the antibody blocked virus infectivity, although it did not precipitate the antigen in agar gel Ouchterlony double diffusion tests. Immuno-electron microscopy confirmed that the antibody coats virions but does not cause clumping. The antibody may be a useful tool for investigating coat protein-dependent initiation of ilarvirus infection.
American Type Culture Collection, Rockville, Md.
J A
JA
Aebig
R L
RL
Jordan
R H
RH
Lawson
H T
HT
Hsu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002213:Capsid / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D009500:Neutralization Tests; D010942:Plant Viruses / Q000276:immunology* / Q000648:ultrastructure; D014771:Virion / Q000276:immunology
D000911:Antibodies, Monoclonal