A simple method for quantitative determination of bacterial adherence to human and animal epithelial cells.
A simple quantitative method for determining bacterial adherence to epithelial cells was devised. The method involved incubation of fluorescein-labeled bacteria with oral epithelial cells. Non-adherent bacteria were subsequently removed by Percoll density centrifugation. The degree of bacterial adherence was then determined fluorospectrophotometrically by measuring the fluorescein extracted from the adherent bacteria. The degree of adherence corresponded well with the microscopic counts of adherent bacteria. The method proved to be suitable for assaying adherence of both gram-positive and gram-negative bacteria to human as well as animal epithelial cells.
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität, Giessen, Federal Republic of Germany.
P
P
Valentin-Weigand
G S
GS
Chhatwal
H
H
Blobel
D000818:Animals; D001422:Bacterial Adhesion; D004848:Epithelium / Q000382:microbiology; D019793:Fluorescein; D005452:Fluoresceins; D006090:Gram-Negative Bacteria; D006094:Gram-Positive Bacteria; D006801:Humans; D066298:In Vitro Techniques; D009061:Mouth Mucosa / Q000382:microbiology*; D013053:Spectrophotometry
D005452:Fluoresceins; D019793:Fluorescein