Determination of the formaldehyde content in fishery products.
The influence of external factors, such as storage temperature and time on the content of free formaldehyde (FA) in fishery products is described. On the basis of the examination of several methods for the determination of free and bound FA, the following procedures are recommended: (1) for measuring free FA, the samples are extracted using 6% perchloric acid at room temperature and the FA content of the extracts is measured by the formation of 3,5-diacetyl-1,4-dihydrolutidine; (2) bound, acid-labile FA is released by steam distillation using 1% sulphuric acid, giving a pH value of about 1. The FA content of the distillates may be determined either by chromotropic acid assay or by the method described for the extracts.
Institut für Biochemie und Technologie, Bundesforschungsanstalt für Fischerei, Hamburg, Federal Republic of Germany.
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005399:Fishes / Q000378:metabolism*; D005557:Formaldehyde / Q000032:analysis*; D007202:Indicators and Reagents; D009282:Naphthalenesulfonates
D000596:Amino Acids; D007202:Indicators and Reagents; D009282:Naphthalenesulfonates; D005557:Formaldehyde; C020996:chromotropic acid