Neurohypophyseal hormone-like peptides in the ovine pineal gland using reverse-phase liquid chromatography and radioimmunoassay.
A method is described for the determination of the neurohormone contents of ovine pineal tissue by radioimmunoassay (RIA) after successive fractionation on gel filtration in formic acid and reverse-phase liquid chromatography (HPLC). This method gives a good resolution for the neurohormones vasopressin, vasotocin and oxytocin, without a significant interference of aspecific cross-reacting of peptides with the RIA. An acid extract from ovine pineal tissue was found to contain amounts of immunoreactive AVP- and OXT-like peptides, whereas an AVT-like peptide was not detectable over background levels after HPLC with post-column RIA. It is concluded from our results that an AVT-like peptide is not present in ovine pineal tissue, and the pineal AVP- and OXT-like peptides appeared to be associated to neurophysin molecules.
Department of Organic Chemistry, University of Utrecht, The Netherlands.
H P
HP
Noteborn
A C
AC
Reinharz
P
P
Pévet
I
I
Ebels
C A
CA
Salemink
D000818:Animals; D001127:Arginine Vasopressin / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010121:Oxytocin / Q000302:isolation & purification; D010870:Pineal Gland / Q000032:analysis*; D010909:Pituitary Hormones, Posterior / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods; D012756:Sheep
D010909:Pituitary Hormones, Posterior; D001127:Arginine Vasopressin; D010121:Oxytocin