Induction of an hsp70-like protein during organ culture of rat embryonic heart.
Hearts were isolated from rat embryos on Day 12 of gestation and incubated in vitro at 37 degrees C in the presence of [35S]methionine; the labeled proteins were then analyzed by two-dimensional gel electrophoresis. The results showed that an acute and transient synthesis of an hsp70 (heat-shock proteins with a M.W. of about 70,000)-like protein was induced in the isolated rat embryonic hearts, without the concomitant total inhibition of normal cellular protein synthesis usually observed in other heat-shock systems. The significance of the results to the embryonic stress hypothesis of teratogenesis recently proposed is discussed.
Higo
H
H
Department of Geneticopathology, Hiroshima University, Japan.
Higo
K
K
Satow
Y
Y
Lee
J Y
JY
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006321:Heart / Q000196:embryology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D007525:Isoelectric Focusing; D009206:Myocardium / Q000378:metabolism*; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats
D006360:Heat-Shock Proteins