Isoparametric analysis of binding and partitioning processes.
An isoparametric method is described for the analysis of titration data pertaining to the binding of a ligand to an acceptor, or to the partition of a molecule between lipid and aqueous compartments. Where spectroscopic titrations are employed, the analysis does not require a priori choice of an association model, nor assumptions concerning a linear relationship between the spectroscopic signal and the amount of bound or partitioned ligand. The method has wide applicability and is illustrated with reference to (a) ligand-acceptor associations monitored by perturbation of the absorption or fluorescence signal of ligand or acceptor, and (b) cross-linking interactions such as those encountered in antigen-antibody precipitin reactions.
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
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RC
Chatelier
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WH
Sawyer
D000937:Antigen-Antibody Reactions; D001671:Biochemistry / Q000379:methods*; D008024:Ligands; D008433:Mathematics; D008956:Models, Chemical; D012995:Solubility; D013050:Spectrometry, Fluorescence
D008024:Ligands