The mouse eye lens obsolescence (Elo) mutant: studies on crystallin gene expression and linkage analysis between the mutant locus and the gamma-crystallin genes.
Previous studies showed that the mouse Elo (eye lens obsolescence) mutation is located on chromosome 1, at a site near the Len-1 locus, which is defined by a set of polymorphic gamma-crystallin proteins. To investigate further the relationship between Elo and the gamma-crystallins, we have examined the steady-state levels of gamma-crystallin transcripts in normal and mutant eyes and analyzed the linkage relationship between the Elo locus and the gamma-crystallin genes. Our data showed that, while gamma-crystallin mRNA levels are preferentially reduced in the mutant eyes, the mutation does not seem to map within the gamma-crystallin gene cluster. The distance between Elo and the gamma 6 gene (the most proximal gamma-crystallin gene member) is estimated to be 1.4 +/- 0.9 cM, whereas that between gamma 6 and the distantly linked gamma 2 gene is 2.7 +/- 1.3 cM. Our data also suggest the possibility of recombination hot spots with the gamma-crystallin gene cluster.
Department of Genetics, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
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P
Quinlan
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Oda
M L
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Breitman
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LC
Tsui
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D003459:Crystallins / Q000235:genetics*; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D007908:Lens, Crystalline / Q000002:abnormalities*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D010375:Pedigree; D014158:Transcription, Genetic
D003459:Crystallins