A nonenzymatic method for determination of hydrogen peroxide and organic peroxides.
Reduction of hydrogen peroxide and organic peroxides (t-butyl hydroperoxide and linoleic acid hydroperoxide) was achieved with homovanillic acid as hydrogen donor in the presence of the triethylenetetramine-Fe3+ complex. By the catalytic action of this complex, homovanillic acid is oxidized to its fluorescent dimer. Based on this reaction a fluorometric method for the measurement of the hydroperoxides mentioned above is described. The method can be extended to the determination of substrate-enzyme systems that produce hydrogen peroxide, e.g., glucose-glucose oxidase. The method allows the determination of substances such as hydrogen peroxide and t-butyl hydroperoxide with an accuracy and precision of less than 3%. Glucose can be determined with similar precision and an accuracy of 4.7%.
Institut für Chemie, Universität für Bodenkultur, Vienna, Austria.
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R
Ebermann
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Couperus
D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D007202:Indicators and Reagents; D007700:Kinetics; D008041:Linoleic Acids / Q000032:analysis*; D008054:Lipid Peroxides / Q000032:analysis*; D010545:Peroxides / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D020122:tert-Butylhydroperoxide
D007202:Indicators and Reagents; D008041:Linoleic Acids; D008054:Lipid Peroxides; D010545:Peroxides; C008285:linoleic acid hydroperoxide; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase