The action of pertussis toxin on heart adenylate cyclase and lipolysis in the parametrial adipose tissue of the rat.
Pertussis toxin is now used as an experimental tool for the study of receptor-mediated inhibitory systems. Based on our previous work, where we demonstrated in rats pretreated with pertussis toxin the blockade of negative chronotropic and reduction of negative inotropic effects of carbamoylcholine, we examined the effects of cholinergic agents on the rat heart adenylate cyclase activity. Pertussis toxin pretreatment reduced the inhibitory effects of cholinergic agents on adenylate cyclase activity only very slightly. This contrasts to the strong antagonistic effect on the phenylisopropyladenosine-induced inhibition of adrenergic lipolysis, including that increased lipolysis after 48 hours starvation. These data suggest that the strong inhibition of negative chronotropic effect of muscarinic drugs by pertussis toxin may be related to the recently described No protein rather then Ni protein from the adenylate cyclase complex.
Department of Pharmacology, Faculty of General Medicine, Charles University, Prague, Czechoslovakia.
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D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D005260:Female; D006321:Heart / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects*; D009206:Myocardium / Q000201:enzymology; D010095:Oxotremorine / Q000494:pharmacology; D037342:Pertussis Toxin; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D037361:Adenylate Cyclase Toxin; D010566:Virulence Factors, Bordetella; D010660:Phenylisopropyladenosine; D010095:Oxotremorine; D002217:Carbachol; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases; D007545:Isoproterenol