Preproenkephalin mRNA in neuroblastoma X glioma, NG 108-15, hybrid cells and in parental cell lines: mouse neuroblastoma, N18, and rat glioma, C6.
Preproenkephalin mRNA was analyzed in Neuroblastoma X Glioma, NG 108-15, cells as well as in respective parental cell lines, i.e. mouse Neuroblastoma N18 and rat Glioma C6, using a rat preproenkephalin cDNA as a probe. NG synthesize efficiently a preproenkephalin mRNA, similar in size to that of normal tissues. Of the two parental cell lines, Neuroblastoma seems to synthesize it as well as NG; conversely Glioma cell lines under the same conditions do not appear to synthesize preproenkephalin mRNA with the same efficiency as NG and N18.
Dipartimento di Chimica Organica e Biologica, Universita' di Napoli, Italy.
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A
Palmisano
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D'Auria
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Sannia
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G
Marino
G
G
Tocco
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004745:Enkephalins / Q000235:genetics*; D005910:Glioma; D006822:Hybrid Cells / Q000378:metabolism*; D051379:Mice; D009447:Neuroblastoma; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats
D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D004247:DNA; C033660:preproenkephalin