Apolipoprotein composition of bovine lipoproteins isolated by gel filtration chromatography.
1. Bovine lipoproteins were isolated from plasma by gel filtration and apolipoprotein composition determined by SDS-polyacrylamide gel electrophoresis. 2. Bovine triglyceride-rich lipoproteins contained a novel low mol. wt protein Mr = 22,000 and low mol. wt proteins that may be analogous to non-ruminant apolipoproteins A-I, A-IV, and E. 3. Apolipoprotein C appeared to be a minor constituent of bovine triglyceride-rich lipoproteins. 4. Triglyceride-rich lipoproteins contained two high mol. wt proteins of approx. Mr = 220,000 and 290,000. 5. The predominant bovine low density lipoprotein apolipoprotein was approx. Mr = 290,000, however, greater then 25 proteins were often observed between Mr = 110,000 and 370,000. 6. Bovine high density lipoprotein contained proteins analogous to apolipoprotein A-I and C apolipoproteins. 7. Differences in apolipoprotein profiles between non-lactating and lactating cows were not apparent.
Department of Dairy Science, University of Wisconsin, Madison 53706.
R R
RR
Grummer
C A
CA
Meacham
W L
WL
Hurley
C L
CL
Davis
D000818:Animals; D001053:Apolipoproteins / Q000097:blood*; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007774:Lactation / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008970:Molecular Weight; D011247:Pregnancy
D001053:Apolipoproteins; D008077:Lipoproteins, LDL