An assay for the detection of human anti-murine immunoglobulins in the presence of CA125 antigen.
A radioimmunoassay for the detection of human immunoglobulin response is described which is suitable for the screening of patients undergoing immunotherapy with murine monoclonal antibodies. The use and the sensitivity of the assay are illustrated in the case of patients undergoing diagnostic imaging for ovarian cancer with the monoclonal antibody, OC125. The method offers the distinct advantage of quantitating human anti-murine antibody in serum containing a high concentration of antigen recognized by the injected murine antibody. In addition, the assay may be generalized to screen patients undergoing immunotherapy with a variety of murine or other monoclonal antibodies.
Moseley
K R
KR
Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Boston, MA.
Knapp
R C
RC
Haisma
H J
HJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000627:therapeutic use*; D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000276:immunology*; D015295:Antigens, Tumor-Associated, Carbohydrate; D002277:Carcinoma / Q000276:immunology / Q000628:therapy; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D051379:Mice; D010051:Ovarian Neoplasms / Q000276:immunology / Q000628:therapy; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D012044:Regression Analysis
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate