Immunohistology of clotting factor V in human extraembryonic membranes.
Blood clotting Factor V was studied on fixed and unfixed cryostat sections of term human placentae, basal plates, amniochorions, and first-trimester placentae by using one- and two-colour immunofluorescence. A monoclonal antibody (GB36) which reacts with cytotrophoblast was used to identify cytotrophoblast. Factor V was identified in villous cytotrophoblast of term chorionic villi and very weakly or not at all in first-trimester chorionic villi. Other extra-embryonic tissues were non-reactive with anti-Factor V. These data show that Factor V is a useful marker of term villous cytotrophoblast. Immunopathological consequences of this are considered.
INSERM U210, Faculte de Medecine, Nice, France.
B L
BL
Hsi
W P
WP
Faulk
C J
CJ
Yeh
J A
JA
McIntyre
D000650:Amnion / Q000378:metabolism; D002824:Chorionic Villi / Q000378:metabolism; D005165:Factor V / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D014018:Tissue Distribution; D014327:Trophoblasts / Q000378:metabolism*
D005165:Factor V