Monoclonal antibody GB17 recognizes human syncytiotrophoblast.
A monoclonal antibody, GB17, was obtained from a hybridoma produced following murine immunization with isolated human term syncytiotrophoblastic microvilli. Immunohistological studies demonstrated the GB17 antigen to be present on syncytiotrophoblast of human term, first- and second trimester placenta, but not on human extravillous trophoblast or baboon placental trophoblast. Other normal adult human tissues and transformed cell lines tested were non-reactive with this antibody. Radio-iodinated term syncytiotrophoblastic microvillous protein was immunoprecipitated by GB17, and shown by sodium dodecyl sulfate--polyacrylamide gel electrophoresis to migrate as a single protein band at 175 kDa. The restricted tissue specificity suggests that the GB17 antigen could be useful for the development of a contragestational vaccine.
Hsi
B L
BL
INSERM U210, Faculté de Médecine, Nice, France.
Yeh
C J
CJ
Johnson
P M
PM
Bereford
N
N
Stern
P L
PL
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification; D066246:ErbB Receptors / Q000276:immunology; D005260:Female; D006801:Humans; D008871:Microvilli / Q000276:immunology; D008970:Molecular Weight; D011247:Pregnancy; D014018:Tissue Distribution; D014327:Trophoblasts / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D066246:ErbB Receptors