Hypothermic effect of neuromedin N in mice and its potentiation by peptidase inhibitors.
The intracerebroventricular administration of neuromedin N (from 50 ng to 5 micrograms) elicited a dose- and time-dependent hypothermia in mice. Two aminopeptidase inhibitors, bestatin (50 micrograms) and puromycin (50 micrograms), the endopeptidase 24.11 inhibitor, thiorphan (10 micrograms), and the angiotensin-converting enzyme inhibitor, captopril (50 micrograms), were tested for their ability to potentiate the neuromedin N-induced hypothermia. Only bestatin significantly increased the response to the peptide. In addition, thiorphan, though devoid of effect on the neuromedin N-induced hypothermia when given alone, further potentiated the response elicited by neuromedin N and bestatin. The combinations of puromycin/thiorphan and bestatin/captopril did not potentiate the neuromedin N-induced hypothermia.
Dubuc
I
I
Unité de Neuropsychocharmacologie Expérimentale, U.A. 1170 du CNRS, Saint Etienne du Rouvray, France.
Nouel
D
D
Coquerel
A
A
Menard
J F
JF
Kitabgi
P
P
Costentin
J
J
D000818:Animals; D001831:Body Temperature / Q000187:drug effects*; D004357:Drug Synergism; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D009496:Neurotensin / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*
D010446:Peptide Fragments; D011480:Protease Inhibitors; C042090:neuromedin N; D009496:Neurotensin