Dome formation in cell cultures of chick embryo retinal pigment epithelium and effects of ouabain and 2,4-dinitrophenol thereon.
Cells from chick embryo retinal pigment epithelium were cultured on glass slips. The primary culture cells formed confluent cell layers which were studded with a number of domes. The domes had usually appeared by the fourth day of culture and were susceptible to 3 X 10(-6) M ouabain and 10(-4) M 2,4-dinitrophenol, indicating that fluid was transepithelially transported from the apical to the basal side by means of an energy-requiring and ouabain-sensitive mechanism. Other than domes, small blisters appeared after 4-10 days of culture. They were also susceptible to the metabolic inhibitors.
Department of Ophthalmology, Japan.
K
K
Kishida
A
A
Kiritoshi
T
T
Sasabe
K
K
Matsumoto
R
R
Manabe
D019297:2,4-Dinitrophenol; D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D004140:Dinitrophenols / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D004140:Dinitrophenols; D010042:Ouabain; D019297:2,4-Dinitrophenol