Genetic control of liver alcohol dehydrogenase expression in inbred mice.
The expression of the mouse Adh-1 gene which encodes the ADH-AA isoenzyme of liver alcohol dehydrogenase is under genetic control. The content of alcohol dehydrogenase in the C57BL inbred strain of mice is about twice that in DBA mice. In order to examine the mechanism for control of liver alcohol dehydrogenase expression in mice, we have isolated liver RNA from high and low enzyme activity strains and quantified the content of ADH mRNA with the ADH-A cDNA that was recently cloned in our laboratory. The content of ADH-A mRNA in liver correlates well with enzyme activity in the mouse strains; hence, the expression of Adh-1 appears to be under transcriptional control. A restriction fragment polymorphism was found in mouse liver DNA which correlates with the content of ADH-AA in the high and low ADH activity strains.
Department of Medicine, Indiana University School of Medicine, Indianapolis 46223.
L S
LS
Patterson
K
K
Zhang
H J
HJ
Edenberg
W F
WF
Bosron
D000426:Alcohol Dehydrogenase / Q000096:biosynthesis*; D000818:Animals; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA
D007527:Isoenzymes; D000426:Alcohol Dehydrogenase