[Amino acid specificity of the phosphoprotein kinase activity of various fractions of non-histone chromatin proteins from the kidney, hepatoma and liver of rats after a single dose of diethylnitrosamine].
Amino acid specificity of phosphorylation of some nuclear non-histone proteins was studied in the fractions, eluted by 0.4-0.5 M NaCl from phosphocellulose columns, using the reaction of 33P transfer, catalized by native phosphoprotein kinase, from gamma-33P-ATP into the proteins. A number of amino acid residues, capable to reaction, were phosphorylated in the 0.4-0.5 M NaCl fraction of non-histone proteins from kidney of intact rats, from cells of hepatoma 27 and from liver cells after a single intraperitoneal administration of diethylnitrosamine. At the same time, methionine residue was markedly phosphorylated in the fraction of 0.4-0.5 M NaCl of intact rat kidney, whereas this effect was not found in the preparations of hepatoma 27 and in cells of "carcinogenous" liver tissue. As distinct from kidney non-histone proteins, proteins from the 0.4-0.5 M NaCl fraction of the latter preparations tended to exhibit tyrosine phosphokinase activity. It is well known that many of oncoproteins exhibited mainly the tyrosine-specific phosphoprotein kinase activity. The data obtained suggest that "tyrosine" shift in the preparations of non-histone proteins from tumoral cells or from rat liver cells, altered under the effect of hepatocarcinogenesis, may be an indication of functional activity of oncogenes.
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Kushner
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002843:Chromatin / Q000201:enzymology; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004052:Diethylnitrosamine; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D009928:Organ Specificity; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
D000596:Amino Acids; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D010750:Phosphoproteins; D004052:Diethylnitrosamine; D011494:Protein Kinases
Aminokislotnaia spetsifichnost' sobstvennoĭ fosfoproteinkinaznoĭ aktivnosti nekotorykh fraktsiĭ negistonovykh belkov khromatina pochek, gepatomy i pecheni krys pri odnokratnom deĭstvii diétilnitrozamina.