DNA repair by articular chondrocytes. V. O6 methylguanine-acceptor protein activity in resting and cultured rabbit and human chondrocytes.
The level of O6-methylguanine acceptor protein activity was examined in rabbit and human articular chondrocytes of different ages. The activity per microgram of DNA in rabbit chondrocytes was 5-fold lower than in humans. There was no age-dependent decrease in the activity of resting or cultured chondrocytes of either species. The values for resting cells were comparable to those of cultured cells. The lack of age-related differences in methyltransferase activity, in contrast to nucleotide excision repair [Mech. Ageing Dev. 32 (1985) 39-55], indicates that separate repair systems behave differently with respect to chronological aging. The methyl transferase activity may be more essential for survival of articular chondrocytes and therefore more highly conserved with age.
Department of Pathology, State University of New York, Stony Brook 11794.
J M
JM
Lipman
L
L
Sokoloff
R B
RB
Setlow
D000375:Aging; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D002478:Cells, Cultured; D004260:DNA Repair; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism; D019853:O(6)-Methylguanine-DNA Methyltransferase; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D013045:Species Specificity
D011506:Proteins; D006147:Guanine; C008449:O-(6)-methylguanine; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase