[Transport and intracellular distribution of components of the cortisol-asialotranscortin complex in the liver].
The effect of desialation of human transcortin on the transcortin-cortisol complex transport into hepatocytes and on subsequent intracellular distribution of the complex components was studied in model experiments with perfused rat liver. It was demonstrated that in the presence of desialated transcortin the time of [3H]cortisol incubation in the perfusion medium decreased more than 200 times as compared with the native protein. [3H]cortisol and [131I]asialotranscortin trapping by hepatocytes occurs simultaneously. The content of the [3H]cortisol--[131I]asialotranscortin complex in rat liver plasma membranes reaches a maximum 3 min after beginning of perfusion. Then the intact complex is transported into lysosomes, where it dissociates with a subsequent release of asialotranscortin and cortisol metabolites into the cytoplasm.
N
N
Karásková
K
K
Bezouska
L
L
Stárka
R
R
Hampl
O
O
Tàborsky
D000818:Animals; D001212:Asialoglycoproteins; D001692:Biological Transport; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014156:Transcortin / Q000031:analogs & derivatives* / Q000378:metabolism
D001212:Asialoglycoproteins; C020707:asialotranscortin; D014156:Transcortin; D006854:Hydrocortisone
Transport i vnutrikletochnoe raspredelenie komponentov kompleksa kortizol-asialotranskortin v pecheni.