[Selectivity of cell degeneration and histological peculiarities of the repair process in the developing rat spinal cord after ethylnitrosourea administration].
We observed the selectivity of cell degeneration and histological peculiarities of the repair process in the lesion of developing rat spinal cord. Each of the 21 pregnant rats was given a single injection of ethylnitrosourea (ENU), 60 mg/kg body weight, in the tail vein on embryonic day 16. Each fetus was removed surgically from the dam every 2 hours for 24 hours and every 24 hours for 5 days. These fetuses were fixed by the transcardial perfusion to observe them with light and electron microscope. Degeneration was induced selectively in certain cells of the proliferating phase of the matrix cell layer and in the migrating neuroblasts in the alar plate. Individually affected cells were eliminated by phagocytes in the pre-existing extracellular space. No reactive gliosis, fibrosis, abnormal vascularization or infiltration of granulocytes and lymphocytes was observed at any time.
Oyanagi
K
K
Department of Neuropathology, Niigata University, Japan.
Yoshida
Y
Y
Ikuta
F
F
D000818:Animals; D005038:Ethylnitrosourea / Q000009:adverse effects*; D005260:Female; D008854:Microscopy, Electron; D009410:Nerve Degeneration / Q000187:drug effects*; D009416:Nerve Regeneration / Q000187:drug effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000187:drug effects / Q000196:embryology* / Q000473:pathology; D013118:Spinal Cord Diseases / Q000139:chemically induced* / Q000473:pathology
D005038:Ethylnitrosourea