Inhibition of human leukocyte 5-lipoxygenase by 15-HPETE and related eicosanoids.
The inhibition of human leukocyte 5-lipoxygenase by 15-hydroperoxyeicosatetraenoic acid and its chemical or enzymatic rearrangement products was investigated. 15-Hydroperoxyeicosatetraenoic acid was the most potent inhibitor tested. The inhibition was found to be time dependent and is not due to chemical or enzymatic decomposition products nor metabolism of 15-hydroperoxyeicosatetraenoic acid to 5,15-dihydroperoxyeicosatetraenoic acid.
Department of Pharmaceutical Chemistry and Liver Center, University of California, San Francisco 94115.
J R
JR
Cashman
C
C
Lambert
E
E
Sigal
D001091:Arachidonate Lipoxygenases / Q000037:antagonists & inhibitors*; D001095:Arachidonic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D007700:Kinetics; D015289:Leukotrienes; D008054:Lipid Peroxides / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D008054:Lipid Peroxides; D016859:Lipoxygenase Inhibitors; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D001091:Arachidonate Lipoxygenases