Identification of an autonomously replicating sequence near a histone gene of Physarum polycephalum.
Fragments of DNA which function as autonomous replication sequences in yeast were cloned from Physarum polycephalum. The ars activity is located in a 1.2 kbp fragment extending 1.5 kbp to 2.7 kbp upstream of the 5' end of a histone H4 gene. Our recent finding that a replication origin is located at a distance less than 3 kbp of this histone gene suggests that the ars element identified coincides with a specialized replication origin and can be used to direct chromosome replication in Physarum polycephalum.
Wilhelm
M L
ML
Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Demouveau
M F
MF
Wilhelm
F X
FX
D003001:Cloning, Molecular; D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008040:Genetic Linkage; D006657:Histones / Q000235:genetics*; D010804:Physarum / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid
D004271:DNA, Fungal; D006657:Histones