Role of lipid peroxidation in the toxicity of T-2 toxin.
Recent reports suggest that lipid peroxidation may be involved in the toxicity of T-2 toxin. In the present study the influence of T-2 toxin on two parameters of lipid peroxidation was examined: the formation of thiobarbituric acid reactive material in isolated hepatocytes and liver homogenates from rats and ethane exhalation in vivo. In isolated hepatocytes there was no significant increase in thiobarbituric acid reactive material, neither after addition of T-2 toxin in vitro nor when the toxin had been applied to the rats 15 hr before preparation of hepatocytes. In liver homogenates the amount of thiobarbituric acid reactive material was increased up to 50% over the controls, depending on the dose of T-2 toxin. The increased values are difficult to interpret, because the extent of the increase depends on the method used for determination of thiobarbituric acid reactive material. Measuring another parameter of lipid peroxidation, i.e. ethane exhalation, there was no difference between the T-2 toxin treated rats and the controls whereas carbon tetrachloride treated rats exhaled high amounts of ethane. These results suggest that lipid peroxidation does not play a major role in T-2 toxin toxicity.
Schuster
A
A
Walther-Straub-Institut für Pharmakologie und Toxikologie, Universität München, F.R.G.
Hunder
G
G
Fichtl
B
B
Forth
W
W
D000818:Animals; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002251:Carbon Tetrachloride / Q000633:toxicity; D002849:Chromatography, Gas; D004980:Ethane / Q000378:metabolism; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000633:toxicity*; D013605:T-2 Toxin / Q000633:toxicity*; D013858:Thiobarbiturates / Q000494:pharmacology
D008054:Lipid Peroxides; D012717:Sesquiterpenes; D013858:Thiobarbiturates; D002084:Butylated Hydroxytoluene; D002251:Carbon Tetrachloride; D013605:T-2 Toxin; D004980:Ethane