Deoxycytidine is salvaged not only into DNA but also into phospholipid precursors.
Deoxycytidine metabolism was investigated in light density human tonsillar lymphocytes using 5-3H-deoxycytidine as extracellular precursor. A significant portion of the deoxycytidine (more than 50% of ethanol soluble pool) was found to incorporate into dCDP-choline and dCDP-ethanolamine beside the well-known pathway i.e. incorporation into DNA in form of dCMP and dTMP. Hydroxyurea increased the labeling of the deoxyliponucleotides from 5-3H-deoxycytidine in spite of its inhibition of DNA synthesis.
Institute of Biochemistry I., Semmelweis University Medical School, Budapest, Hungary.
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Spasokukotskaja
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Spyrou
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Staub
D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D003841:Deoxycytidine / Q000378:metabolism*; D003843:Deoxycytidine Monophosphate / Q000378:metabolism; D006801:Humans; D006918:Hydroxyurea / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010766:Phosphorylation; D013938:Thymidine Monophosphate / Q000378:metabolism; D013997:Time Factors
D010743:Phospholipids; D003841:Deoxycytidine; D003843:Deoxycytidine Monophosphate; D013938:Thymidine Monophosphate; D004247:DNA; D006918:Hydroxyurea