Effect of ammonium as nitrogen source on production of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase by Cephalosporium acremonium C-10.
Cephalosporin production by Cephalosporium acremonium strain C-10 was suppressed when the organic nitrogen source (1.2% L-asparagine) was replaced by 1.2% (NH4)2SO4. A higher level of (NH4)2SO4 (3.5%) led to even greater suppression. Ammonium repression was exerted on formation of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase, together with that of expandase; a lesser effect by ammonium was observed on cyclase production. Inhibition of ACV synthetase activity byammonium was also observed (ca. 50% inhibition at 250 mM NH4+).
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139.
J Y
JY
Zhang
S
S
Wolfe
A L
AL
Demain
D000164:Acremonium / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism*; D001216:Asparagine / Q000378:metabolism; D002511:Cephalosporins / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D009842:Oligopeptides / Q000096:biosynthesis; D010453:Peptide Synthases / Q000378:metabolism*
D002511:Cephalosporins; D009842:Oligopeptides; C011638:5-(2-aminoadipyl)cysteinylvaline; D001216:Asparagine; D000641:Ammonia; D010453:Peptide Synthases; C095368:alpha-aminoadipyl-cysteinyl-valine synthetase; D009584:Nitrogen