Half-lives of L-[35S]methionine and L-[3H]fucose of transferrin in the serum of rats.
The half-lives of 35S-labelled L-methionine and of 3H-labelled L-fucose of serum transferrin of rats were measured in pulse-chase experiments in vivo. Both L-[35S]methionine and L-[6-3H]fucose disappeared from transferrin with nearly the same half-lives of 33.8 h and 36.5 h, respectively. The data show that in this major serum glycoprotein peripheral carbohydrates and the protein moiety are degraded as a unit.
Institut für Molekularbiologie und Biochemie, Freien Universität, Berlin, West Germany.
D
D
Josić
R
R
Tauber
W
W
Hofmann
J
J
Mauck
W
W
Reutter
D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005470:Fluorometry; D005643:Fucose / Q000378:metabolism*; D006207:Half-Life; D008297:Male; D008715:Methionine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014168:Transferrin / Q000097:blood / Q000276:immunology / Q000493:pharmacokinetics*
D014168:Transferrin; D005643:Fucose; D008715:Methionine