A rapid method for analyzing the ligation products of synthetic oligodeoxyribonucleotides.
A method is described for the rapid analysis of DNA ligation products in the assembly of synthetic genes and gene fragments. The method is based on the simultaneous analysis of multiple ligation reactions where a single but different DNA oligomer is radiolabelled per ligation reaction. After each ligation the reaction mixture is electrophoresed on a denaturing, as well as a non-denaturing, polyacrylamide gel allowing one to monitor the ligation reaction products. In addition, a unique method for generating single stranded DNA sizing standards up to approximately 300 nucleotides in length is described.
Connaught Laboratories, Willowdale, Ontario, Canada.
L U
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Tan
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Glick
D001483:Base Sequence; D004277:DNA, Single-Stranded; D004591:Electrophoresis, Polyacrylamide Gel; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000138:chemical synthesis; D012015:Reference Standards
D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides