Doxycycline effects on the adherence of polymorphonuclear leukocytes to an albumin-coated glass surface.
The effect of doxycycline on polymorphonuclear leukocyte (PMN) adherence to albumin-coated glass surfaces was studied in the absence and presence of the chemotactic peptide FMLP. Three concentrations of doxycycline were studied, one subtherapeutic (0.1 micrograms/ml), one therapeutic (1.0 micrograms/ml) and one supertherapeutic (10 micrograms/ml). PMN adherence was maximal after incubation for 5 min. FMLP did not affect PMN adherence in the present assay system and at the concentration studied (10(-7)M). PMN adherence remained stable and unaffected in the tested doxycycline concentrations. Thus, the present study could not confirm the reported and challenged doxycycline inhibition of PMN adherence.
Dahlgren
C
C
Department of Medical Microbiology, University Hospital, Linköping, Sweden.
Norberg
B
B
Eriksson
S
S
D000418:Albumins; D002448:Cell Adhesion / Q000187:drug effects; D004318:Doxycycline / Q000008:administration & dosage / Q000494:pharmacology*; D005898:Glass; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects*
D000418:Albumins; D009240:N-Formylmethionine Leucyl-Phenylalanine; D004318:Doxycycline