Cloning, physical mapping and cross-hybridization of the canine adenovirus types 1 and 2 genomes.
Cloned HindIII fragments of the type 1 and type 2 canine adenovirus (canAd) genomes were mapped with respect to the BamHI, EcoRI, HincII, PaeR7 and PstI restriction endonuclease cleavage sites. Considering the large differences found between the canAd-1 and canAd-2 DNA molecules, in terms of number and location of restriction sites, cross-hybridization experiments were performed. Homologous DNA sequences were located on the canAd-1 and canAd-2 physical maps. Both viruses are genetically related to the extent of 57%. Our results confirm the existence of two distinct Ad species in the dog.
Jouvenne
P
P
Centre de Recherche en Virologie, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada.
Dion
M
M
Hamelin
C
C
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004285:Dogs; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization