Kinetics of p-nitrophenol mineralization by a Pseudomonas sp.: effects of second substrates.
The kinetics of simultaneous mineralization of p-nitrophenol (PNP) and glucose by Pseudomonas sp. were evaluated by nonlinear regression analysis. Pseudomonas sp. did not mineralize PNP at a concentration of 10 ng/ml but metabolized it at concentrations of 50 ng/ml or higher. The Ks value for PNP mineralization by Pseudomonas sp. was 1.1 micrograms/ml, whereas the Ks values for phenol and glucose mineralization were 0.10 and 0.25 micrograms/ml, respectively. The addition of glucose to the media did not enable Pseudomonas sp. to mineralize 10 ng of PNP per ml but did enhance the degradation of higher concentrations of PNP. This enhanced degradation resulted from the simultaneous use of glucose and PNP and the increased rate of growth of Pseudomonas sp. on glucose. The Monod equation and a dual-substrate model fit these data equally well. The dual-substrate model was used to analyze the data because the theoretical assumptions of the Monod equation were not met. Phenol inhibited PNP mineralization and changed the kinetics of PNP mineralization so that the pattern appeared to reflect growth, when in fact growth was not occurring. Thus, the fitting of models to substrate depletion curves may lead to erroneous interpretations of data if the effects of second substrates on population dynamics are not considered.
Department of Agronomy, Cornell University, Ithaca, New York 14853.
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Schmidt
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Scow
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Alexander
D001673:Biodegradation, Environmental; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D009596:Nitrophenols / Q000378:metabolism*; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D012044:Regression Analysis; D012988:Soil Microbiology
D009596:Nitrophenols; D005947:Glucose; C024836:4-nitrophenol