Isolation and characterization of a highly phosphorylated troponin from bovine heart.
A modified procedure for isolation of troponin from bovine heart is described, which results in a stable and highly phosphorylated protein. 31P-NMR spectra show up to four phosphoserine signals indicating that at least four serine residues of cardiac troponin are phosphorylated in the intact organ. The hydrodynamic parameters of phosphotroponin are almost identical to those previously published. Characteristically cardiac troponin shows a strong tendency to associate that is dependent on protein concentration. Mg2+ may specifically induce an aggregation, which can be observed during sedimentation. This phenomenon seems to be analogous to the Mg2+-induced dimerization of cardiac troponin C [Jaquet, K. and Heilmeyer, L. M. G., Jr (1987) Biochem. Biophys. Res. Commun. 145, 1390-1396]. Upon Mg2+ saturation a shift of one of the four 31P-NMR signals is observed. The affinity of troponin to Ca2+ is reduced when the protein concentration is enhanced only in the presence of Mg2+. This effect of Mg2+ suggests a model for the regulation of the Ca2+-binding affinity of cardiac troponin.
Abteilung für Biochemie Supramolekularer Systeme, Ruhr-Universität Bochum, Federal Republic of Germany.
N
N
Beier
K
K
Jaquet
K
K
Schnackerz
L M
LM
Heilmeyer
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008274:Magnesium / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000032:analysis*; D010768:Phosphoserine / Q000032:analysis; D014336:Troponin / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D014461:Ultracentrifugation
D014336:Troponin; D010768:Phosphoserine; D008274:Magnesium; D002118:Calcium