Simple gas chromatographic method for the determination of medicagenic acid in alfalfa (Medicago sativa).
A gas chromatographic method for the determination of medicagenic acid in alfalfa leaves, stems, entire plant (tops) and roots and also in leaf protein concentrates is described. The method is based on extraction of lucerne saponins followed by hydrolysis of the triterpene glycosides. After derivatization of the liberated sapogenins to silylated products, the trimethylsilylated medicagenic acid was determined by gas chromatography. The sensitivity of the method permits the detection of 50 ng of medicagenic acid.
Rao
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Laboratoire des Xénobiotiques, I.N.R.A., Toulouse, France.
Bories
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D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D000455:Medicago sativa / Q000032:analysis*; D012502:Sapogenins / Q000032:analysis; D012503:Saponins / Q000032:analysis; D014315:Triterpenes / Q000032:analysis*
D012502:Sapogenins; D012503:Saponins; D014315:Triterpenes; C005982:medicagenic acid