Guinea pig MSEL-neurophysin. Sequence comparison of eight mammalian MSEL-neurophysins.
The amino acid sequence of guinea pig MSEL-neurophysin has been determined using tryptic peptides derived from the performic acid-oxidized protein and staphylococcal proteinase peptides obtained from the reduced-carboxamidomethylated neurophysin. Guinea pig MSEL-neurophysin consists of a 93-residue polypeptide chain that shows 12 substitutions and 2 deletions when compared to bovine MSEL-neurophysin. It displays the highest number of variations among known mammalian MSEL-neurophysins. These variations are mainly found in the C-terminal region (residues 88-93). Moreover guinea pig MSEL-neurophysin, like rat homologous protein, exhibits substitutions in positions 2, 5, 29 and 81 and lacks an arginine in the penultimate position. Comparison between eight mammalian MSEL-neurophysins reveals a highly conserved region (residues 1 to 88) and a hypervariable region (residues 89 to 93/95). On the other hand the eight species examined are endowed with arginine vasopressin except pig, which has a lysine vasopressin. In the vasopressin-MSEL-neurophysin precursor, the hormonal moiety and the MSEL region of neurophysin (residues 1-9) are encoded by a common exon in ox, rat and man; it can be concluded that this exon is evolutionarily conservative in contrast to the one encoding the C-terminal region of MSEL-neurophysin.
Laboratory of Biological Chemistry, University of Paris VI, France.
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D000595:Amino Acid Sequence; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis*; D002417:Cattle; D006168:Guinea Pigs / Q000502:physiology*; D006736:Horses; D006801:Humans; D008969:Molecular Sequence Data; D009481:Neurophysins / Q000032:analysis*; D010121:Oxytocin; D010449:Peptide Mapping; D010904:Pituitary Gland, Posterior / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis*; D051381:Rats; D012756:Sheep; D013552:Swine; D014907:Whales
D009481:Neurophysins; D011498:Protein Precursors; D001127:Arginine Vasopressin; D010121:Oxytocin