Loss of sexual differentiation of rat hepatocytes in short-term culture.
We investigated the maintenance of alcohol dehydrogenase (ADH) and estrogen receptors in primary cultures of rat hepatocytes. Female hepatocytes partially lose ADH activity and completely lose estrogen receptors during 4 days in culture. Growth hormone, which induces both the enzyme and receptor in vivo, did not induce either in vitro.
Dept. of Medicine, Indiana Univ. School of Medicine, Indianapolis 46223.
J E
JE
Roepke
D W
DW
Crabb
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008099:Liver / Q000032:analysis / Q000166:cytology*; D008297:Male; D051381:Rats; D011960:Receptors, Estrogen / Q000032:analysis; D012727:Sex Characteristics
D011960:Receptors, Estrogen; D000426:Alcohol Dehydrogenase