Chemical characteristics of follicular glycosaminoglycans.
The two glycosaminoglycans found in FF, DS and HS were isolated from fluid of atretic and non-atretic bovine follicles and simultaneously separated by anion exchange high performance liquid chromatography. Heparan sulfate eluted with the apex of the peak at .071 M NaCl. Dermatan sulfate eluted with the apex of the peak at .353 M NaCl. The concentrations of DS and HS were greater in atretic follicles compared to non-atretic follicles and decreased as follicles size increased, as previously reported. The proportion of DS was approximately 90% of the total glycosaminoglycan concentration of all FF samples. The ratio of galactosamine to glucosamine in DS increased as follicle size increased, being 3.44 +/- .35, 7.65 +/- 2.4, and 15.9 +/- 3.8 (P less than .05) for small, medium and large follicles, respectively.
Department of Dairy Science, University of Wisconsin-Madison.
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D000818:Animals; D002417:Cattle; D003871:Dermatan Sulfate / Q000032:analysis; D004187:Disaccharides / Q000032:analysis; D005260:Female; D005496:Follicular Atresia; D006025:Glycosaminoglycans / Q000032:analysis*; D006107:Granulosa Cells / Q000032:analysis; D006497:Heparan Sulfate / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006080:Ovarian Follicle / Q000032:analysis* / Q000166:cytology
D003871:Dermatan Sulfate; D004187:Disaccharides; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006595:Hexosamines