Doxycycline effects on the adherence of polymorphonuclear leukocytes to an albumin-coated glass surface.
The effect of doxycycline on polymorphonuclear leukocyte (PMN) adherence to albumin-coated glass surfaces was studied in the absence and presence of the chemotactic peptide FMLP. Three concentrations of doxycycline were studied, one subtherapeutic (0.1 micrograms/ml), one therapeutic (1.0 micrograms/ml) and one supertherapeutic (10 micrograms/ml). PMN adherence was maximal after incubation for 5 min. FMLP did not affect PMN adherence in the present assay system and at the concentration studied (10(-7)M). PMN adherence remained stable and unaffected in the tested doxycycline concentrations. Thus, the present study could not confirm the reported and challenged doxycycline inhibition of PMN adherence.
Department of Medical Microbiology, University Hospital, Linköping, Sweden.
C
C
Dahlgren
B
B
Norberg
S
S
Eriksson
D000418:Albumins; D002448:Cell Adhesion / Q000187:drug effects; D004318:Doxycycline / Q000008:administration & dosage / Q000494:pharmacology*; D005898:Glass; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects*
D000418:Albumins; D009240:N-Formylmethionine Leucyl-Phenylalanine; D004318:Doxycycline