Anti-BoLA-w8 monoclonal antibody: production of a tissue typing reagent after blocking monomorphic sites on bovine mononuclear cells.
A murine monoclonal antibody, WIM-8 with specificity for BoLA-w8, was generated after using an immunization protocol designed to reduce the response to bovine monomorphic determinants. BoLA-w8/w9 peripheral blood mononuclear (PBM) cells were incubated with an antiserum from mice immunized with BoLA-w10/w20 PBM cells. Splenocytes from a mouse that received three intravenous injections of the treated cells were fused with murine myelomas. A hybridoma supernatant with alloreactivity in ELISA against BoLA-w8 positive but not BoLA-w8 negative PBM cells was tested for lymphocytotoxicity in parallel with parous alloantisera. WIM-8, an IgG2b monoclonal antibody, identified the 44,000 dalton protein of the bovine MHC class I molecule present on Bos taurus and Bos indicus animals.
Department of Veterinary Science, University of Wisconsin, Madison.
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Splitter
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Burkholder
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O'Reilly
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Janzer
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000917:Antibody Formation; D002417:Cattle / Q000276:immunology*; D007114:Immunization; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight
D000911:Antibodies, Monoclonal