A hydroxyproline-rich protein in the soybean cell wall.
A cDNA clone that hybridizes to an mRNA (1A10) that accumulates to a substantial level in the axis of the germinating soybean seed was sequenced. The amino acid sequence of the clone indicates an almost perfect repeat of Pro-Pro-Val-Tyr-Lys resulting in a protein containing 40% proline and lacking serine and histidine. On the likelihood that such a protein might be a hydroxyproline-rich cell-wall glycoprotein (HRGP), cell walls of a soybean cell culture were extracted by procedures used to obtain soluble basic cell-wall glycoproteins, and the proteins were fractionated and purified. A 33-kDa protein (and possibly a 28-kDa protein) was obtained that has an amino acid distribution similar to that of the cDNA clone. The protein lacks histidine and serine and contains 20% hydroxyproline and 20% proline. The HRGP is thus distinct both in its amino acid content and in its pentameric repeat of Pro-Pro-Val-Tyr-Lys, with half of the prolines being hydroxylated.
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111.
V
V
Averyhart-Fullard
K
K
Datta
A
A
Marcus
D000595:Amino Acid Sequence; D001483:Base Sequence; D002473:Cell Wall / Q000032:analysis*; D004247:DNA / Q000235:genetics; D006909:Hydroxyproline / Q000032:analysis*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D013025:Glycine max / Q000032:analysis* / Q000235:genetics
D010940:Plant Proteins; D004247:DNA; D006909:Hydroxyproline