Enhanced synthesis and secretion of apolipoprotein E from sciatic nerves of streptozotocin-induced diabetic rats after injury.
To elucidate the pathogenesis of diabetic neuropathy, synthesis and secretion of apolipoprotein E (apo E) from sciatic nerves after injury was studied in normal and streptozotocin-induced diabetic rats. Seven, 14, 28, 45 and 59 days after making crush injury on sciatic nerves with concomitant administration of streptozotocin (50 mg/kg body weight), the nerves were taken out and incubated with [35S]methionine. The [35S]labeled apo E was precipitated with specific antiserum. The amounts of apo E secreted into medium by nerves of diabetic rats were 7 times greater than those of non-diabetic rats 7 days after injury. This enhanced secretion of apo E was relatively selective for this protein, since the ratio of the immunoprecipitable apo E to the TCA preciptitable protein in the medium increased in diabetic rats. Intriguing possibility deduced from these results is that the secretion of apo E is involved in the development of diabetic neuropathy.
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
S
S
Ishibashi
N
N
Yamada
Y
Y
Oka
H
H
Shimano
N
N
Mori
T H
TH
Yoon
M
M
Shimada
Y
Y
Kanazawa
Y
Y
Akanuma
T
T
Murase
D000818:Animals; D001057:Apolipoproteins E / Q000096:biosynthesis* / Q000378:metabolism; D001835:Body Weight; D002499:Centrifugation, Density Gradient; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009409:Nerve Crush; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000378:metabolism* / Q000502:physiology; D013462:Sulfur Radioisotopes / Q000378:metabolism; D013997:Time Factors
D001057:Apolipoproteins E; D013462:Sulfur Radioisotopes