Aberrant DNase I digestion kinetics of nucleosomal core particles from sea urchin sperm.
The pancreatic deoxyribonuclease (DNase I) digestion rates at the susceptible sites on nucleosomal core particles from blastula, gastrula and sperm cells of the sea urchin, Parechinus angulosus, have been determined. Although there are differences in their isohistone composition, the rates of digestion are similar for both embryonic stages. The rates of digestion for sperm core particles are 3-5 times lower than for embryo core particles at the more, and up to 2.5 times lower at the less susceptible sites. An explanation for these differences could be sought in the sperm isohistones H2B which are characterized by N-terminal extensions of 20-25 amino acid residues.
Department of Biochemistry, University of Cape Town, Rondebosch, Republic of South Africa.
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Landsman
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Sewell
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von Holt
D000818:Animals; D004247:DNA / Q000302:isolation & purification; D003850:Deoxyribonuclease I; D004625:Embryo, Nonmammalian; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009698:Nucleoproteins / Q000302:isolation & purification; D009707:Nucleosomes / Q000201:enzymology / Q000378:metabolism*; D012617:Sea Urchins; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity
D006657:Histones; D009698:Nucleoproteins; D009707:Nucleosomes; D004247:DNA; D003850:Deoxyribonuclease I