The induction of chromosomal aberrations and SCE by visible light in combination with dyes. I. The effect of hypoxia during light exposure in unsynchronized Chinese hamster ovary cells, sensitized with acridines.
A comparison has been made between the ability of different acridine compounds to act as sensitizers for visible light (400-700 nm) induced chromosomal aberrations and sister-chromatid exchanges (SCE) in unsynchronized Chinese hamster ovary (CHO) cells. Cells were treated for 20 min with acridines (0.1-5.0 microgram/ml), washed free of excess dye and subsequently exposed to visible light (2 x 40 W/8 W m-2) either in air or in nitrogen for 5-15 min. The 4 acridines tested, proved to be effective sensitizers for the induction of both chromosomal aberrations and SCE by visible light. The most pronounced effect was observed when the light exposure of the fluorochrome-pretreated cells was performed in air. Hypoxic conditions during light exposure reduced the effect dramatically, especially in the case of induced chromosomal aberrations. The order of efficiency for the induction of both chromosomal aberrations and SCE was acridine orange greater than acridine yellow greater than proflavine greater than 3,6-diamino-10-methylacridine. The results are discussed in terms of S-independent versus S-dependent mechanisms for inducing chromosomal alterations and the potential involvement of oxygen-derived free radicals in this process.
Department of Genetics, University of Uppsala, Sweden.
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Uggla
D000165:Acridine Orange / Q000494:pharmacology; D000166:Acridines / Q000494:pharmacology*; D000609:Aminoacridines / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D004396:Coloring Agents; D006224:Cricetinae; D005260:Female; D008027:Light; D010053:Ovary; D010100:Oxygen / Q000494:pharmacology*; D011370:Proflavine / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects
D000166:Acridines; D000609:Aminoacridines; D004396:Coloring Agents; C026620:3,6-diamino-10-methylacridinium; D011370:Proflavine; D000165:Acridine Orange; D010100:Oxygen; C025902:acridine yellow