Binding of 3-carbethoxipsoralen to human serum albumin and human serum: influence of free fatty acids.
Characteristics of the binding of 3-carbethoxipsoralen (3CPS) to human serum albumin (HSA) and serum proteins have been studied. An electrophoretic study showed that the predominant binding protein fraction was albumin, with small binding to globulins. Binding to HSA, studied by equilibrium dialysis, is 75% and characterized by a small saturable number of binding sites (N = 0.27) with a moderate affinity constant (K = 8 X 10(4) M-1). Free fatty acids were shown to decrease 3CPS binding to HSA by a non competitive process.
Font
G
G
Laboratoire de Chimie Analytique II, Faculté de Pharmacie, Université de Paris-Sud, Châtenay-Malabry, France.
Mañes
J
J
Martre
H
H
Prognon
P
P
Mahuzier
G
G
D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism*; D003956:Dialysis; D004588:Electrophoresis, Cellulose Acetate; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D011564:Furocoumarins / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding / Q000187:drug effects; D012709:Serum Albumin / Q000378:metabolism*; D012712:Serum Globulins / Q000378:metabolism
D001798:Blood Proteins; D005230:Fatty Acids, Nonesterified; D011564:Furocoumarins; D012709:Serum Albumin; D012712:Serum Globulins; C017104:3-carbethoxypsoralen