Formation of 8-hydroxyhexadecatrienoic acid by vascular smooth muscle cells.
Smooth muscle cells derived from the human umbilical vein produce four radioactive metabolites when they are incubated in culture with [3H]-12-hydroxyeicosatetraenoic acid. This conversion does not require the addition of an agonist for eicosanoid formation. The main product, which accounts for 60% of the radioactivity converted to these metabolites, has been identified as 8-hydroxyhexadecatrienoic acid.
Department of Biochemistry, University of Iowa, Iowa City 52242.
C
C
Hadjiagapiou
H
H
Sprecher
T L
TL
Kaduce
P H
PH
Figard
A A
AA
Spector
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006880:Hydroxy Acids / Q000096:biosynthesis*; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D006880:Hydroxy Acids; D006893:Hydroxyeicosatetraenoic Acids; C054787:8-hydroxyhexadeca-4,6,10-trienoic acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid