Effect of incubation time and temperature on the interference of digoxin-like immunoreactive substances in digoxin immunoassays.
We have tested the cross-reactivity of digoxin-like immunoreactive substances (DLIS) of cord serum in three commercially available radioimmunoassay systems. We found that there is a marked difference in the specificity of the three antibodies. We were able to eliminate or greatly reduce the interference of DLIS by incubating for 1 h at 37 degrees C. The degree of interference for various cord blood sera is given at three temperature levels and at incubation times ranging from 15 min to 48 h.
Yannakou
L
L
Department of Therapeutics, Faculty of Medicine, University of Athens, Alexandra Hospital, Greece.
Diamandis
E P
EP
Souvatzoglou
A
A
D001798:Blood Proteins / Q000276:immunology*; D002298:Cardenolides; D003429:Cross Reactions; D004077:Digoxin / Q000097:blood* / Q000276:immunology; D006801:Humans; D011863:Radioimmunoassay; D012503:Saponins; D013696:Temperature; D013997:Time Factors
D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin