[Effect of sex-hormone on composition of rat skin surface lipid].
The optimum analytical conditions for studying the composition of skin surface lipid were examined by high-performance liquid chromatography equipped with a photo-diode array detector. Optimum conditions were as follows: ULTRON N-C18 (150 x 4.6 mm) as stationary phase, acetonitrile/tetrahydrofuran/water (55/35/10, V/V) as eluent at the flow rate of 1.0 ml/min, and column temperature of 40 degrees C. The peaks were detected by monitoring the absorbance at 210 nm. Effect of sex-hormone on composition of skin surface lipids was examined. Gonadectomized Sprague-Dawley rats were injected with either testosterone (50 mg/kg, s.c.) or estradiol (5 mg/kg, s.c.) for 12 days. Amount of crude lipid from the skin surface was decreased at 8 days after castration; estradiol dosing to castrated rats also decreased the amount. The other hand, in ovariectomized rats, testosterone injection increased skin surface lipids. It is recognized that sex-hormone dosing after gonadectomy changes the percentage composition of squalene and cholesterol in male rats, but does not charge them in females.
Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Tokyo, Japan.
K
K
Imaeda
K
K
Ohsawa
T
T
Watanabe
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D050356:Lipid Metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D013185:Squalene / Q000378:metabolism; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology*
D013739:Testosterone; D004958:Estradiol; D013185:Squalene; D002784:Cholesterol