Determination of atropine in plasma by a direct radioreceptor assay.
A highly sensitive radioreceptor assay for the anticholinergic atropine was developed and could be applied directly to plasma samples obtained from mini-pigs without any clean-up. Plasma samples were collected during 6 h after atropine was administered intravenously or endobronchially. The endobronchial plasma concentration-time curves were characterized by a very rapid rise of the concentration but with a subsequent much slower decrease than after intravenous administration. This indicates an initially high as well as prolonged uptake from the lungs.
Ensing
K
K
State University of Groningen, Groningen Centre for Drug Research, The Netherlands.
De Zeeuw
R A
RA
Hörnchen
U
U
Schüttler
J
J
Stoeckel
H
H
D000818:Animals; D001285:Atropine / Q000097:blood* / Q000493:pharmacokinetics; D001980:Bronchi; D007275:Injections, Intravenous; D007322:Instillation, Drug; D011869:Radioligand Assay / Q000379:methods*; D013552:Swine
D001285:Atropine