Molecular cloning and sequence analysis of the cDNA for small proteoglycan II of bovine bone.
The cDNA for the full-length core protein of the small chondroitin sulphate proteoglycan II of bovine bone was cloned and sequenced. A 1.3 kb clone (lambda Pg28) was identified by plaque hybridization with a previously isolated 1.0 kb proteoglycan cDNA clone (lambda Pg20), positively identified previously by polyclonal and monoclonal antibody reactivity and by hybrid-selected translation in vitro [Day, Ramis, Fisher, Gehron Robey, Termine & Young (1986) Nucleic Acids Res. 14, 9861-9876]. The cDNA sequences of both clones were identical in areas of overlap. The 360-amino-acid-residue protein contains a 30-residue propeptide of which the first 15 residues are highly hydrophobic. The mature protein consists of 330 amino acid residues corresponding to an Mr of 36,383. The core protein contains three potential glycosaminoglycan-attachment sites (Ser-Gly), only one of which is within a ten-amino-acid-residue homologous sequence seen at the known attachment sites of related small proteoglycans. Comparisons of the published 24-residue N-terminal protein sequence of bovine skin proteoglycan II core protein with the corresponding region in the deduced sequence of the bovine core protein reveals complete homology. Comparison of the cDNA-derived sequences of bovine bone and human embryonic fibroblast proteoglycans shows a hypervariable region near the N-terminus. Nucleotide homology between bone and fibroblast core proteins was 87% and amino acid homology was 90%.
Bone Research Branch, National Institute of Dental Research, Bethesda, MD 20892.
A A
AA
Day
C I
CI
McQuillan
J D
JD
Termine
M R
MR
Young
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones / Q000032:analysis*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000235:genetics*
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D011509:Proteoglycans; D004247:DNA