Liquid chromatographic-fluorometric determination of cinnamyl alcohol in perfumes, colognes, and toilet waters.
A liquid chromatographic (LC) method is described for the determination of cinnamyl alcohol (3-phenyl-2-propen-1-ol) in fragrance compositions. The fragrance product is partially cleaned up by diluting the fragrance with a 95% ethanol-water mixture and passing it through a short column containing RP-8 packing. An aliquot of the effluent is then analyzed by LC using an RP-18 column interfaced to a spectrophotofluorometer equipped with double monochromators. The fluorescence emission intensity of the eluted cinnamyl alcohol is measured and compared with that of a standard to calculate the amount of cinnamyl alcohol present. Recoveries from fragrance products fortified with cinnamyl alcohol at levels ranging from 0.0020 to 0.060 mg/mL ranged from 85 to 105% with a mean of 94%. The lowest level of determination was 0.0005 mg/mL.
Food and Drug Administration, Division of Colors and Cosmetics, Washington, DC 20204.
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HH
Wisneski
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Yates
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Wenninger
D000433:1-Propanol / Q000032:analysis; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D010476:Perfume / Q000032:analysis*; D020005:Propanols; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D010476:Perfume; D020005:Propanols; D012997:Solvents; D000433:1-Propanol; C020722:cinnamyl alcohol