Inactivation of pig muscle enolase by carbohydrate metabolites.
Fourteen carbohydrate metabolites and related compounds were used to investigate the inhibition of enolase from pig muscle. Glucose and fructose decreased the activity very slightly. The degree of inhibition by phosphate derivative was stronger and dependent on the position of phosphate residue. One of the strongest inhibitor was glyceraldehyde-3-phosphate. The presence of inhibitors do not change the affinity to the substrate - 2-phosphoglycerate. The possible physiological role of enolase in carbohydrate metabolism is discussed.
Department of Biochemistry, Medical School, Wroclaw, Poland.
M
M
Niemczuk
E
E
Wolna
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D005246:Feedback; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000037:antagonists & inhibitors*; D013552:Swine
D002241:Carbohydrates; D010751:Phosphopyruvate Hydratase