Isolation and purification of fibrinogen/fibrin degradation products by chromatography on protamine-agarose.
Protamine-agarose chromatography is introduced as a rapid and efficient method for the isolation of fibrinogen/fibrin degradation products from biological fluids such as human plasma. All fibrinogen/fibrin fragments above a MW of 35000, including fragments D and E are quantitatively adsorbed to insolubilized protamine and can be eluted with a buffer containing 0.2 M sodium citrate/citric acid pH 5.3, following previous elution of non-fibrinogen proteins with a buffer containing 0.8 M NaCl at neutral pH. Fragments D and E are separated by stepwise elution. The efficiency of the method is - evaluated by applying it to plasma samples obtained from healthy donors and from patients with clinical and laboratory evidence of disseminated intravascular coagulation.
Dempfle
C E
CE
Fakultät für klinische Medizin Mannheim der Universität Heidelberg, Mannheim, Germany.
Heene
D L
DL
D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005337:Fibrin / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D005340:Fibrinogen / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D011479:Protamines
D011479:Protamines; D005337:Fibrin; D005340:Fibrinogen