DNA synthesis, protein synthesis and platinum content following drug administration in cis-diamminedichloroplatinum(II) -sensitive and -resistant L1210 cells transferred from in vitro to in vivo conditions.
L1210 cells with in vitro induced drug resistance against cis-diamminedichloroplatinum(II) (cis-Pt(II] were inoculated in mice and several times transplanted. Then the effect of cis-Pt(II) on drug-sensitive and drug-resistant L1210 cells in mice was investigated. While the DNA and protein synthesis in drug-sensitive cells after in vivo cis-Pt(II) treatment was inhibited by 50%, that of drug-resistant cells remained virtually unaffected. The content of platinum in drug-sensitive cells was approximately three times higher in comparison with drug-resistant L1210 cells.
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
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E
Hanusovská
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V
Ujházy
D000818:Animals; D002460:Cell Line; D002945:Cisplatin / Q000627:therapeutic use*; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D004351:Drug Resistance; D007939:Leukemia L1210 / Q000188:drug therapy* / Q000235:genetics; D051379:Mice; D008811:Mice, Inbred DBA; D009154:Mutation; D009368:Neoplasm Transplantation; D010984:Platinum / Q000378:metabolism*; D014176:Protein Biosynthesis; D013936:Thymidine / Q000378:metabolism
D004273:DNA, Neoplasm; D010984:Platinum; D002945:Cisplatin; D013936:Thymidine