Ion-pair liquid chromatographic determination of some penicillins in canine and equine sera.
A sensitive liquid chromatographic method was developed for determining oxacillin, cloxacillin, and dicloxacillin (simultaneously), and penicillin G, amoxicillin, carbenicillin, and ticarcillin in canine and/or equine serum. The method involves filtering diluted serum through a 30,000 molecular weight cut-off filter and separating penicillins from other serum components by ion-pair liquid chromatography using a reverse-phase column eluted with acetonitrile-water solutions. The ultraviolet absorbance of the column effluent was monitored at 230 nm. Recoveries of oxacillin, cloxacillin, dicloxacillin, and penicillin G (spiked at 2.5 micrograms/mL), amoxicillin (spiked at 5 micrograms/mL), and carbenicillin and ticarcillin (spiked at 10 micrograms/mL) from canine and equine serums ranged from 78.3 to 104.4% with coefficients of variation ranging from 3.35 to 5.95%. The limit of detection for these penicillins was 0.02-0.05 microgram/mL.
North Carolina State University, College of Veterinary Medicine, Raleigh 27606.
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K
Tyczkowska
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AL
Aronson
D000818:Animals; D002852:Chromatography, Ion Exchange; D004285:Dogs; D006736:Horses; D007202:Indicators and Reagents; D010406:Penicillins / Q000097:blood*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D010406:Penicillins; D012997:Solvents