Determination of ivermectin in milk by high performance liquid chromatography.
A sensitive and specific high performance liquid chromatographic technique is described for the determination of ivermectin in milk. Milk samples (1 ml) were extracted with organic solvent (iso-octane and acetonitrile) and then chromatographed using reversed phase system. Specificity of the assay was confirmed by the use of mass spectrometry. After an subcutaneous administration of ivermectin (0.2 mg/kg) in a cow, ivermectin was detected in milk for about three weeks and over this period milk and plasma concentration were of the same order of magnitude.
Alvinerie
M
M
Laboratoire de Pharmacologie, INRA, Toulouse, France.
Sutra
J F
JF
Galtier
P
P
Toutain
P L
PL
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D007559:Ivermectin / Q000032:analysis*; D008892:Milk / Q000032:analysis*
D007559:Ivermectin