Isolation of mouse isometallothioneins. A comparison of isometallothioneins in growing cells and post-mitotic cells.
Mouse metallothioneins (MTs) were separated into three isoforms by an anion-exchange h.p.l.c. column; conventionally isolated MT-1 and MT-2 showed a single peak (MT-1-1) and two peaks (MT-2-1 and MT-2-2), respectively. In growing cells, developing hepatocytes and growing tumour cells, MT-1/MT-2 ratios were less than 0.6, irrespective of the type of MT inducer, whereas adult liver post-mitotic cells had a ratio of more than 1.0. A large amount of the MT-2-2 subfraction was found in dexamethasone-treated FM3A cells; 90% of MTs was MT-2-2, suggesting that glucocorticoid hormone mainly induces MT-2-2 in tumour cells.
Kobayashi
S
S
Department of Biology, Kyoritsu College of Pharmacy, Tokyo, Japan.
Sayato-Suzuki
J
J
D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008938:Mitosis; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology
D002104:Cadmium; D003907:Dexamethasone; D008668:Metallothionein; D015032:Zinc