Nutritional regulation of milk protein messenger RNA concentrations in mammary acini isolated from lactating rats.
The effect of starvation, starvation and refeeding and feeding a low-protein diet on concentrations of the mRNAs for the alpha-, beta- and gamma-caseins, alpha-lactalbumin and whey acidic protein has been determined using dot-blot hybridization analyses with total RNA extracted from mammary acini isolated from lactating rats. Starvation for 48 h decreased the concentrations of all RNA species to between 5 and 20% of those for rats feeding ad libitum when expressed on a DNA basis. Refeeding for 24h restored the concentrations to control values. Consumption of a low protein diet reduced the concentrations of each mRNA by about 50%. Only minor changes were detected in the mRNA concentrations for alpha-casein, alpha-lactalbumin and whey acidic protein in samples prepared at six hourly intervals from rats receiving a restricted intake (40% ad libitum) of the control diet.
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
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Geursen
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Grigor
D000818:Animals; D002940:Circadian Rhythm; D004032:Diet; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D008894:Milk Proteins / Q000096:biosynthesis*; D009747:Nutritional Physiological Phenomena; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008894:Milk Proteins; D012333:RNA, Messenger