Production of iota toxin by Clostridium spiroforme: a requirement for divalent cations.
The effects of divalent cations (Ca2+, Co2+ and Zn2+) on the production of iota toxin by Clostridium spiroforme were studied. Toxin production had an absolute requirement for one or more cations in the range 1-5 mM. Using bispecific antisera, we showed that production of both the components of the toxin (ia and ib) were enhanced by divalent cations added to brain-heart infusion supplemented with peptone and glucose.
Carman
R J
RJ
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
van Tassell
R L
RL
Wilkins
T D
TD
D036002:ADP Ribose Transferases; D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002118:Calcium / Q000494:pharmacology*; D002413:Cations, Divalent; D003013:Clostridium / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology*; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D015032:Zinc / Q000494:pharmacology*
D001427:Bacterial Toxins; D002413:Cations, Divalent; C054948:iota toxin, Clostridium spiroforme; D003035:Cobalt; D036002:ADP Ribose Transferases; D015032:Zinc; D002118:Calcium