Kinetic modeling of hormone release: application to time-averaged met-enkephalin release.
The time course of hormone concentrations in response to a stimulus can be characterized with kinetic methods to express rates of hormone release and elimination, total amount of hormone release and expected best times for blood sampling. Routine application of kinetic methods should increase the sensitivity and applicability of hormonal challenge tests. Applications to the TRH test and to prolactin release measurements are reviewed. A new kinetic model for continuously collected samples is applied to measurements of met-enkephalin release in rats. Calculations indicated an initial concentration of 1876 pg/ml and a ventricular volume of distribution of 0.25 ml. After exposure of rats to nitrous oxide, the rate of release of met-enkephalin-like activity into the ventricles rose from 3.4 to 8.3 pg/min.
Department of Psychiatry, University of Health Sciences/Chicago Medical School, Illinois 60064.
C M
CM
Swartz
R M
RM
Quock
D000818:Animals; D004744:Enkephalin, Methionine / Q000134:cerebrospinal fluid*; D006728:Hormones / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D009609:Nitrous Oxide / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D006728:Hormones; D004744:Enkephalin, Methionine; D009609:Nitrous Oxide