Separation by counter-current distribution of young-chick erythrocyte populations and numerical resolution of distribution curves.
The presence of two red cell populations in young chicks has been demonstrated after increasing the settling time used during counter-current distribution with charged 5% Dextran--4% poly(ethylene glycol) two-phase systems. A Fortran program using statistical methods was applied to show the resolution in two peaks (with two or three subpopulations assumed) of the counter-current distribution curves.
Ferrer
E
E
Departamento de Bioquimica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Martin
M
M
Alonso
R
R
Luque
J
J
D000818:Animals; D002645:Chickens; D003377:Countercurrent Distribution; D004912:Erythrocytes / Q000032:analysis* / Q000201:enzymology