Simultaneous determination of flurazepam and five metabolites in serum by high-performance liquid chromatography and its application to pharmacokinetic studies in rats.
A reversed-phase high-performance liquid chromatographic method is described which allows the quantification of flurazepam and five of its metabolites with a single, isocratic determination. In addition, it has the advantage of possessing a low detection limit and high precision. A 2 mm I.D. column was used to minimize sample size (50 microliter), increase sensitivity and reduce solvent consumption. The method was used to demonstrate that N-1-desalkylflurazepam, the major metabolite, has a short half-life in the rat in contrast to its prolonged life in humans.
Department of Psychology, Rutgers University, New Brunswick, NJ 08903.
C E
CE
Lau
J L
JL
Falk
S
S
Dolan
M
M
Tang
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005479:Flurazepam / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats
D005479:Flurazepam