[Structural changes in contractile proteins of muscle fibers studied by polarization ultraviolet fluorescence microscopy. X. The effect of ATP, Ca2+, pH change and ionic strength of the washing solution on the structural state of thick filaments].
By the method of polarized ultraviolet fluorescence microscopy, effects of ATP, Ca2+, changes in pH and ionic strength of washing solution of the structural state of thick filaments in both actin-free muscle fibers of rabbit and anisotropic discs (A-discs) of glycerinated fibers of crab were studied. The dependence of tryptophan fluorescence anisotropy of thin filaments upon physico-chemical parameters (compounds) of washing solution has been found. The structural state of thick filaments was suggested to be influenced by ATP, Ca2+, changes in pH and ionic strength of washing solution.
Iu S
IuS
Borovikov
N I
NI
Lebedeva
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D003386:Brachyura; D002118:Calcium / Q000494:pharmacology*; D003592:Cytophotometry / Q000379:methods; D005454:Fluorescence Polarization; D005990:Glycerol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D009994:Osmolar Concentration; D011817:Rabbits; D012996:Solutions
D009124:Muscle Proteins; D012996:Solutions; D000255:Adenosine Triphosphate; D009218:Myosins; D005990:Glycerol; D002118:Calcium
Izuchenie strukturnykh izmeneniĭ sokratitel'nykh belkov myshechnogo volokna s pomoshch'iu poliarizatsionnoĭ ul'trafioletovoĭ fluorestsentnoĭ mikroskopii. X. Vliianie ATF, Ca2+, izmeneniia pH i ionnoĭ sily omyvaiushchego rastvora na strukturnoe sostoianie tolstykh niteĭ.