Cryopreservation of human oocytes.
Three different methods were used for freezing human excess oocytes (320 frozen, 205 thawed) in our IVF programme and the results of these methods were compared. A high fertilization rate (75%) could be achieved after thawing, using 1,2 propanediol as a cryoprotectant. Polyploidy rates of 20% and 40% were observed using DMSO and 1,2-propanediol as cryoprotectants, respectively. Using the ultracooling method, the survival rate was poor (4%).
Universitäts-Frauenklinik Bonn, Bonn-Venusberg, FRG.
S
S
Al-Hasani
K
K
Diedrich
H
H
van der Ven
A
A
Reinecke
M
M
Hartje
D
D
Krebs
D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005615:Freezing; D006801:Humans; D009865:Oocytes / Q000187:drug effects; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000494:pharmacology*; D014021:Tissue Preservation / Q000379:methods*
D003451:Cryoprotective Agents; D011409:Propylene Glycols; D019946:Propylene Glycol; D004121:Dimethyl Sulfoxide