Temperature and external electric field influence in membrane permeability of Babesia infected erythrocytes.
1. Modification of erythrocyte membrane properties infected by Babesia canis was studied using the effect of electric pulses of short duration. 2. This process induces the formation of pores in the membrane and the releasing of hemoglobin and other cytoplasmic proteins into the external medium. 3. The rate of molecular permeation across the electrically perforated membranes depends on several factors: electric-field strength, pulse number, pulse duration, temperature and cellular concentration. 4. Even for low parasitemia, differences in the effect of these parameters were observed between infected and non-infected erythrocytes. 5. Here we describe an influence of electric field intensity and temperatures on the opening pores.
Gneno
R
R
Laboratoire de Biochimie des Membranes et LBTM CNRS UM 380024, Villeubanne, France.
Azzar
G
G
Got
R
R
Roux
B
B
D000818:Animals; D001404:Babesiosis / Q000097:blood*; D001797:Blood Protein Electrophoresis; D002463:Cell Membrane Permeability; D004285:Dogs; D004560:Electricity; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000502:physiology*; D066298:In Vitro Techniques; D013696:Temperature