[Characteristics of ribosomal RNA synthesis in the blood lymphocytes of chronic lympholeukemia patients].
A study was made of the number of silver grains over the nucleoli and of the content of ribosomes in the lymphocyte cytoplasm for six healthy persons and for 20 patients with chronic lymphatic leukemia. Besides ratios of compact, nucleolonemic and ring types of nucleoli were evaluated in addition to counts of the specific radioactivity of mature 28S rRNA in lymphocytes. In the majority of cases examined, cells with 1 or 2 nucleoli of compact and nucleolonemic types were seen dominating. The number of silver grains over the nucleoli in the control healthy persons did not differ from that in patients who did not receive any treatment, which contrasted with high value grain counts in the treated patients. The lymphocyte ribosome contents varied within the normal and decreased values in both the patient groups. The specific radioactivity in 28S rRNA leukemic lymphocytes was significantly lower in groups of patients with low ribosome contents than in those with the normal ones. The data suggest that in the leukemic cells with a high or unaltered activity of ribosome cistrons and low ribosome levels rRNA processing is broken.
Morozova
E O
EO
Mamaev
N N
NN
Blinov
M N
MN
Samuskevich
I G
IG
Medvedeva
N V
NV
D000368:Aged; D002466:Cell Nucleolus / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012339:RNA, Ribosomal, 28S / Q000096:biosynthesis*; D012270:Ribosomes / Q000378:metabolism
D012335:RNA, Ribosomal; D012339:RNA, Ribosomal, 28S
Osobennosti sinteza ribosomnoĭ RNK v limfotsitakh krovi bol'nykh khronicheskim limfoleĭkozom.