Solid phase synthesis of a 31-residue fragment of human glucose-dependent insulinotropic polypeptide (GIP) by the continuous flow polyamide method.
A fragment, GIP1-31, of the human Glucose-dependent Insulinotropic Polypeptide (GIP1-42) Tyr-Ala-Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His- Gln-Gln-Asp-Phe-Val-Asn-Trp-Leu-Leu-Ala-Gln-Lys-Gly has been synthesized by solid phase methodology under continuous flow conditions. The peptide was assembled on a polydimethylacrylamide kieselguhr support by using 9-fluorenylmethoxycarbonyl amino acid pentafluorophenyl esters. The crude peptide, obtained after trifluoroacetic acid cleavage, was purified by gel filtration and by reverse-phase HPLC. This synthetic replicate, corresponding to human GIP1-31, retains the ability of naturally occurring GIP to stimulate insulin release.
Department of Biochemistry II, Karolinska Institute, Stockholm, Sweden.
M
M
Carlquist
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes; D005749:Gastric Inhibitory Polypeptide / Q000138:chemical synthesis*; D006801:Humans; D009757:Nylons / Q000138:chemical synthesis; D010446:Peptide Fragments / Q000138:chemical synthesis*; D010455:Peptides / Q000032:analysis*
D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D009757:Nylons; D010446:Peptide Fragments; D010455:Peptides; D005749:Gastric Inhibitory Polypeptide