Sensitive homologous radioimmunoassay for human parathyroid hormone to diagnose hypoparathyroid conditions.
A sensitive radioimmunoassay for human parathyroid hormone (hPTH) to diagnose hypoparathyroid conditions is described. The antiserum was raised in goats against extracted hPTH. Synthetic human PTH fragment (53-84) is used as a standard and 125I-Tyr52hPTH (53-84) as a tracer. After a two-step incubation (24 h + 24 h) at 4 degrees C, the bound and free fractions are separated by a mixture of second antibody and polyethylene glycol solution. The detection limit for hPTH (53-84) is 2 pmol/L hPTH (53-84) (2 mol/tube). The PTH level in 36 healthy subjects was 5-12 pmol/L. Of 14 patients with hypoparathyroidism 11 patients had PTH concentrations below normal, two patients had levels on the lower limit of the normal range (5 pmol/L). The concentration of one patient with pseudohypoparathyroidism was markedly elevated. This assay is suitable for detecting low PTH levels and for studying changes of PTH concentration within the normal range.
Abteilung für Allgemeine Chirurgie, Universität Heidelberg, FRG.
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D
Zillikens
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FP
Armbruster
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J
Stern
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H
Schmidt-Gayk
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F
Raue
D002118:Calcium / Q000097:blood; D005069:Evaluation Studies as Topic; D006801:Humans; D007011:Hypoparathyroidism / Q000097:blood / Q000175:diagnosis*; D010281:Parathyroid Hormone / Q000097:blood*; D011863:Radioimmunoassay
D010281:Parathyroid Hormone; D002118:Calcium