Use of a colorimetric microtiter (MTT) assay in determining the radiosensitivity of cells from murine solid tumors.
We assessed the use of a colorimetric assay for determination of radiosensitivity for cells taken directly from murine solid tumors. The assay uses microtier plates and measures the ability of viable cells to reduce a tetrazolium salt (MTT) to an insoluble form, a formazan salt. We established the dependency of the assay on the cell number and time of assay for two murine tumors (EMT-6 and RIF-1). We compared the MTT assay to the standard clonogenic assay and had good agreement of surviving fraction after radiation doses of 2 and 4 Gy. It is possible, therefore, to adapt the MTT assay for use with cell suspensions prepared directly from fresh murine tumors. This may provide a methodology for the determination of the clinical radiosensitivity of tumors including fresh clinical tumor specimens.
MRC Unit University Department of Clinical Oncology and Radiotherapeutics, Cambridge, England.
T H
TH
Wasserman
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Twentyman
D000818:Animals; D002470:Cell Survival; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D051379:Mice; D009374:Neoplasms, Experimental / Q000532:radiotherapy*; D011836:Radiation Tolerance; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue