Lethal effect of glucose load on malignant cells.
Ehrlich ascites tumor (EAT) cells were treated by glucose load under anoxic conditions (for 15 or 60 min) and/or by gamma-radiation (20 Gy). The efficiency of the treatment was judged from the tumorigenic activity of EAT cell inocula. The markedly increased efficiency of the combined treatment of EAT cells by glucose load in anoxia and by gamma-radiation is due to the additive action of both agents. The glucose load in anoxia leads to extensive desintegration of tumor cells. Further, the lethal effect of various pH values on EAT cells was investigated. Different pH values were obtained by means of both glucose load and phosphate buffers. The effect was investigated by determining the tumorigenic activity of EAT cells tested in vivo in mice and by determining the radiosensitivity of treated EAT cells. The results allowed us to conclude that the same values of pH lead to the same effect on EAT cells independently of the way by which the given pH value was reached.
All-Union Cancer Research Center, Academy of Medical Sciences, Moscow.
N L
NL
Shmakova
K
K
Laser
T E
TE
Fomenkova
V I
VI
Korogodin
S
S
Kozubek
S P
SP
Yarmonenko
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology* / Q000532:radiotherapy; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010100:Oxygen; D011836:Radiation Tolerance
D005947:Glucose; D010100:Oxygen