Secretion of S-100 from rat C6 glioma cells.
Conditioned media from rat C6 glioma cells contain significant levels of S-100 immunoreactivity as determined by radioimmunoassay. The levels of extracellular S-100 detected could not be accounted for by the release of intracellular S-100 into the media from lysed cells. The extracellular form of S-100 exhibits fractionation properties and immunological characteristics that are different from those of the intracellular form of S-100 in C6 cells. While the intracellular S-100 levels increase as a function of days in culture, the extracellular S-100 levels are high until the cells reach confluency and are lower in postconfluent cultures. Altogether, our data suggest that C6 glioma cells secrete S-100, and that the quantitative levels of the intracellular and secreted forms of S-100 are differentially regulated.
Van Eldik
L J
LJ
Department of Cell Biology, Vanderbilt University, Nashville, TN 37232.
Zimmer
D B
DB
D000818:Animals; D005910:Glioma / Q000032:analysis / Q000378:metabolism* / Q000473:pathology; D011863:Radioimmunoassay; D051381:Rats; D009418:S100 Proteins / Q000032:analysis / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D009418:S100 Proteins