Protective effectiveness of an endotoxin-depleted pertussis vaccine.
Pertussis vaccine depleted of endotoxin by the polymyxin-Sepharose affinity chromatography method was tested for toxic activity and protective effectiveness in mice. Preparations containing 1000-fold and 1 000 000-fold less endotoxin fulfilled the established experimental criteria for freedom from toxicity. A fourfold concentrate of the former demonstrated a protection rate only 10% less than that of standard, untreated pertussis vaccine.
Department of Bacteriology, Hospital for Sick Children, Toronto, Ontario, Canada.
R M
RM
Bannatyne
J
J
Jackowski
D000818:Animals; D001681:Biological Assay; D001835:Body Weight / Q000187:drug effects; D002846:Chromatography, Affinity; D004339:Drug Compounding; D004731:Endotoxins; D051379:Mice; D010567:Pertussis Vaccine / Q000276:immunology* / Q000633:toxicity
D004731:Endotoxins; D010567:Pertussis Vaccine