Relationship between reestablishment of sarcolemma-glycocalyx ultrastructures and restoration of transmembrane potentials in cultured rat heart cells.
Simultaneous studies on sarcolemma-glycocalyx ultrastructures and electrophysiological properties of the trypsin-released rat heart cells were carried out in order to define the relationship between sarcolemmal membrane repair and transmembrane potential recovery in the long-term cultured heart cells. Based on electron microscopic observations of the trypsinized heart cells maintained in the long-term culture, serial alterations of sarcolemma-glycocalyx complex could be divided into three successive stages. A defective stage of the sarcolemma-glycocalyx complex was present in the cultured cells between day 3 and day 6 of incubation. A repaired stage of the sarcolemma-glycocalyx complex was observed in the cells from day 7 to day 9 of incubation. A well-organized stage of the sarcolemma-glycocalyx complex was seen in the cells after ten days of incubation. Sequential measurements of electrophysiological parameters of the cultured heart cells showed a diphasic evolution of maximum diastolic potential and action potential amplitude, with an initial decrease from day 3 to day 9 of incubation and a later return to normal range after ten days of incubation. There seemed a good correlation between electrophysiological properties and sarcolemma-glycocalyx ultrastructures. Thus, we conclude that restoration of the electrophysiological properties of the cultured cells is closely related to the reorganization of the defective sarcolemmal membrane.
Department of Internal Medicine, Chang Gung Memorial Hospital, Taipei, Taiwan, R.O.C.
Y S
YS
Lee
T S
TS
Hsu
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D006023:Glycoproteins / Q000032:analysis*; D006321:Heart / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure*; D011134:Polysaccharides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012508:Sarcolemma / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D014357:Trypsin
D006023:Glycoproteins; D011134:Polysaccharides; D014357:Trypsin