High-performance liquid chromatographic drug analysis by direct injection of whole blood samples. III. Determination of hydrophobic drugs adsorbed on blood cell membranes.
The determination of strongly hydrophobic drugs in whole blood by high-performance liquid chromatography was investigated and the amount adsorbed on cytomembranes was measured. A polyvinyl resin, TSK Gel HW-65, was used as the pre-column packing. Proteins, cytomembranes and endogenous hydrophilic components flowed through the pre-column in aqueous medium, but strongly hydrophobic substances such as chlorpromazine were adsorbed and then eluted by backflushing into an ODS analytical column. The recovery of chlorpromazine from whole blood was 103.3% with a coefficient of variation of 3.4% (n = 10). This method gave the total amounts in blood, representing not only the amount bound to proteins but also that bound to cytomembranes. The difference in the concentrations determined in whole blood and the supernatant of haemolysed whole blood gave the value adsorbed on cytomembranes.
Faculty of Pharmaceutical Sciences, Fukuyama University, Japan.
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Tamai
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Yoshida
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Imai
D000327:Adsorption; D001773:Blood Cells / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002746:Chlorpromazine / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood*; D006435:Renal Dialysis; D013997:Time Factors
D004364:Pharmaceutical Preparations; D002746:Chlorpromazine