Yellow lupin cytoplasmic tRNAGlu is not a cofactor in chlorophyll biosynthesis.
Yellow lupin seeds (Lupinus luteus) cytoplasmic tRNAGlu was isolated and the primary structure was determined to be: (sequence in text) AGU CCCGGCGACGGAACCAOH. It is 76 nucleotides long and contains 8 modified nucleosides: 2 residues of pseudouridine, ribothymidine, 3 dihydrouridines, 5-methylcytosine and 1-methyladenosine. This tRNAGlu assayed in delta-aminolevulinic acid synthesis was shown to be inactive. Its structural features are discussed.
Barciszewska
M
M
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
Barciszewski
J
J
D001483:Base Sequence; D002734:Chlorophyll / Q000096:biosynthesis*; D003593:Cytoplasm / Q000378:metabolism / Q000502:physiology; D007887:Fabaceae / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D012345:RNA, Transfer, Amino Acid-Specific / Q000502:physiology*; D012352:RNA, Transfer, Glu / Q000302:isolation & purification / Q000502:physiology*
D012345:RNA, Transfer, Amino Acid-Specific; D012352:RNA, Transfer, Glu; D002734:Chlorophyll