Metabolic behaviour of a stable DNA-protein complex.
1. A stable DNA-protein complex resisting all treatments dissociating noncovalently associated proteins was isolated from mouse erythroleukemia cells. 2. Two-dimensional tryptic peptide mapping of this DNA-linked protein component revealed a remarkable similarity to the maps of the corresponding proteins from other mammalian chromatins. 3. Labelling experiments showed that the protein component was metabolically stable.
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Z
Z
Avramova
I
I
Mikhailov
R
R
Tsanev
D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping; D014316:Tritium
D002250:Carbon Radioisotopes; D004273:DNA, Neoplasm; D003852:Deoxyribonucleoproteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D014316:Tritium