Copper-induced lipid peroxidation and hemolysis in whole blood: evidence for a lack of correlation.
In order to establish a possible relationship between hemolytic and peroxidant activities of copper ions, lipid peroxidation was studied in plasma and whole blood incubated for 24 h with different concentrations of copper. The lipid peroxidation was investigated by the determination of thiobarbituric acid-reactive species, conjugated dienes and fluorescent lipid chromophores. The copper-induced lipoperoxidation was clearly demonstrated in plasma incubated with high concentrations of copper (12.10(-4) and 20.10(-4) M); in whole blood, all the lipoperoxidation products were increased in the plasma, while the fluorescent lipid chromophores remained unchanged in red cells. With a copper concentration similar to that found in acute copper intoxication (4.10(-4) M) no lipoperoxidation was observed and yet hemolysis occurred, reduced glutathione (GSH) decreased dramatically and methemoglobin (MetHb) increased. From these results, we assume that, despite its prooxidant activity and its capacity to produce lipoperoxidation, it has not been proven that copper ions at pathophysiological concentrations induce hemolysis by an oxidative mechanism.
Laboratoire de Biochimie et de Toxicologie, Faculté de Médecine et de Pharmacie, Poiters, France.
A
A
Piriou
C
C
Tallineau
S
S
Chahboun
R
R
Pontcharraud
O
O
Guillard
D000328:Adult; D003300:Copper / Q000097:blood / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005978:Glutathione / Q000097:blood; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008054:Lipid Peroxides / Q000096:biosynthesis* / Q000097:blood; D008706:Methemoglobin / Q000378:metabolism; D013054:Spectrophotometry, Atomic
D005231:Fatty Acids, Unsaturated; D006454:Hemoglobins; D008054:Lipid Peroxides; D003300:Copper; D008706:Methemoglobin; D005978:Glutathione