Water binding of proteins in the processing frankfurter-type sausages. Part. 1. Water-binding ability of freeze-dried meat fractions containing myofibrillar and stromal proteins.
As soon as possible and 48 h after slaughter respectively, from both blade-bone muscle groups of cattle and pig carcasses the "thick pieces" were excised, extracted, and fractionated. Residues and precipitates from water and salt extracts resulted were freeze-dried, and an improved Baumann capillary suction apparatus was used to measure their water binding capacity (WBC) with and without addition of 2% sodium chloride and/or heating to 80 degrees C. With one exception the WBC results followed a relative pattern demonstrating the final residues (stromal proteins and leavings of myofibrillar proteins) binding the highest amount of added water, precipitates of dialysis (mainly containing myofibrillar proteins) a remarkable amount and powdered meats the least. As scanning electron micrographs confirmed, there were no fibrous structures in the precipitates resulted from dialysis of salt solutions (1.0 mol/1). Heating decreased the spontaneous water uptake of all fractions. Addition of sodium chloride had only a noticeable capillary-suction and swelling effect on unheated samples. Hence swelling of undissolved protein structures (extraction of myosin and possibly of actomyosin) is therefore not the only way for water binding in frankfurter-type sausages.
Heinevetter
L
L
Central Institute of Nutrition, Potsdam-Rehbrücke, GDR.
Gassmann
B
B
Kroll
J
J
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005612:Freeze Drying; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D009124:Muscle Proteins / Q000032:analysis*; D011485:Protein Binding; D013552:Swine; D014867:Water / Q000032:analysis*
D009124:Muscle Proteins; D014867:Water