Patterning of the head in hydra as visualized by a monoclonal antibody, II. The initiation and localization of head structures in regenerating pieces of tissue.
The body column of hydra is polarized such that a new head will regenerate from the apical end when both extremities are removed. This is due to a graded property of the tissue termed the head activation gradient. The aim of the experiments presented here was to determine what events connect a two-dimensional segment of the activation gradient in an isolated piece of tissue with the formation of a head structure at a particular location. To this end, tissue pieces with three different shapes were excised and analyzed during and after regeneration. The most apical tissue of each piece was labeled with the DNA-intercalating dye, DAPI, and the area where developmental changes were occurring was monitored using the monoclonal antibody CP8 (Javois et al., 1986). First, it was shown that polarity of regeneration was maintained regardless of the fraction of body length included in the excised pieces. Second, while head structures usually formed from the original apical tissue, they could be located anywhere in the regenerate. This was an effect of the healing process which shaped the apical edge differently in different pieces. Third, early CP8 binding occurred in similarly shaped areas suggesting that patterning events were initiated in a contiguous manner wherever apical tissue was located. And finally, not all of the CP8-marked tissue successfully formed structures. Apparently some regions were favored to continue the patterning process, and these in turn extinguished the process in neighboring regions.
Javois
L C
LC
Department of Biology, Catholic University of America, Washington, D.C. 20064.
Bode
P M
PM
Bode
H R
HR
D000818:Animals; D000911:Antibodies, Monoclonal; D006829:Hydra / Q000502:physiology / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008297:Male; D012038:Regeneration
D000911:Antibodies, Monoclonal