Leptospiral attachment to extracellular matrix of mouse fibroblast (L929) cells.
The attachment of leptospires to the extracellular matrix (ECM) remaining after mouse fibroblast (L929) cells on coverslips had been solubilized with Triton X-100 was examined. Each highly virulent line of Leptospira interrogans serovar copenhageni, canicola and pomona attached to ECM more effectively than intermediately virulent and avirulent lines of the same strains, suggesting a correlation between virulence and attachment to ECM. Inhibition of the attachment of highly virulent copenhageni to ECM was found in the presence of the homologous immunoglobulin G Fab fragment.
Department of Hygiene and Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
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Ito
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Yanagawa
D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D005109:Extracellular Matrix / Q000382:microbiology*; D005347:Fibroblasts; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007921:Leptospira interrogans / Q000472:pathogenicity / Q000502:physiology*; D051379:Mice; D013696:Temperature; D014774:Virulence
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G