Simple and rapid spectrophotometric method for determination of adrenaline and isoprenaline.
A simple, rapid, and specific method for determination of adrenaline bitartrate and isoprenaline sulfate was developed. The method is based on the oxidation reaction in aqueous solution of either adrenaline bitartrate or isoprenaline sulfate in the presence of silver oxide to give a red aminochrome measurable at 490 nm. The color is stable for 2 h. Beer's law is valid within a concentration range of 5-80 micrograms/mL for each drug. All variables were studied to optimize the reaction conditions. The method is specific for catecholamine drugs having a secondary amine in the side chain. Other catecholamines such as orciprenaline and noradrenaline do not interfere, and no interference was observed in the presence of common pharmaceutical adjuvants. Interference due to sodium metabisulfite and sodium chloride was circumvented. The validity of the method was tested by analyzing adrenaline injections and isoprenaline tablets. Good recoveries were obtained for these preparations. The results were comparable to those obtained by official procedures. The proposed method is also recommended as a stability indicating assay for oxidative degradation of both drugs.
University of Assiut, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Egypt.
S R
SR
el-Shabouri
S A
SA
Hussein
A A
AA
Abdel-Alim
D055598:Chemical Phenomena; D002621:Chemistry; D004837:Epinephrine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007545:Isoproterenol / Q000032:analysis*; D009638:Norepinephrine / Q000032:analysis; D010084:Oxidation-Reduction; D010087:Oxides / Q000032:analysis; D012834:Silver / Q000032:analysis; D018030:Silver Compounds; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D010087:Oxides; D018030:Silver Compounds; D012997:Solvents; D013607:Tablets; D012834:Silver; C040225:disilver oxide; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine