Gross and ultrastructural development of the saccule of the toadfish Opsanus tau.
The development of the saccule of the inner ear in the toadfish was studied using light and scanning electron microscopy. Development was studied from the early embryo (2-3 days postfertilization), when the otocyst first forms, to the early-aged juvenile when the development of the inner ear approximates that of the adult (4 weeks postfertilization). The ultrastructural features examined included the morphological sequence of ciliary bundle growth, the development of orientation patterns of the ciliary bundles, and the relation of the ultrastructural development to overall gross development. Gross development may be divided into four distinct morphological stages. Stage I encompasses the time from initial formation of the otocyst until the start of stage II, which is the stage when the pars inferior begins migrating ventrally. In stage III the pars inferior continues to elongate ventrally. Stage IV starts when the pars inferior elongates in a rostral and caudal direction. The ear attains its adult shape in stage IV. The differentiation of the sensory cells begins during stage I. During the early part of stage I, a small cilium is found on the apical surface of each cell throughout the otocyst. In the middle and late periods of stage I, a few microvillous buds add to the surface of the cells that already have a kinocilium. These early ciliary bundles are clustered on the rostral-ventral and caudal walls of the otocyst. There is no clear patterning to the orientation of these ciliary bundles. In stage II the ventral stretching of the labyrinth wall causes a spreading of the clustered bundles along the ventral and medial walls of the pars inferior. The orientation of the ciliary bundles has no distinct pattern. In stage III the orientations of the ciliary bundles appear adultlike, although there are so few ciliary bundles that it is difficult to make a definite determination. During stage IV, hair cells with an adultlike horizontal and vertical orientation pattern are found on the rostral and caudal sections of the saccular macula, respectively. The transition region lying between these areas has ciliary bundles with various orientations.
Department of Anatomy and Cell Biology, Georgetown University School of Medicine, Washington, DC 20007.
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BH
Sokolowski
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Popper
D000818:Animals; D002923:Cilia / Q000648:ultrastructure; D007758:Ear, Inner / Q000196:embryology / Q000254:growth & development; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology; D005399:Fishes / Q000196:embryology* / Q000254:growth & development; D006198:Hair Cells, Auditory / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D012444:Saccule and Utricle / Q000166:cytology / Q000196:embryology* / Q000254:growth & development / Q000648:ultrastructure