Cloning and characterization of tetM gene from a Ureaplasma urealyticum strain.
We have cloned a 4.9-kilobase (kb) HincII fragment which contains a tetracycline resistance determinant (tetM) from the chromosome of Ureaplasma urealyticum. The 4.9-kb HincII fragment contains DNA in addition to the structural gene, is closely related to the previously characterized 5.0-kb fragment from pJI3, and contains no normal ureaplasmal DNA sequences.
Department of Pathobiology, University of Washington, Seattle 98177.
J T
JT
Brown
M C
MC
Roberts
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D013753:Tetracycline Resistance / Q000235:genetics*; D014509:Ureaplasma / Q000187:drug effects / Q000235:genetics*
D004269:DNA, Bacterial