Temperature-related release of serotonin from unrestrained rats' pre-optic area perfused with ethanol.
The circumscribed effect of ethyl alcohol on the local efflux of serotonin (5-HT) within the thermosensitive region of the anterior hypothalamic, pre-optic area (AH/POA) of the unrestrained rat was examined in relation to core temperature. A single guide cannula for push-pull perfusion was implanted stereotaxically in the AH/POA within coronal planes AP 7.0-8.2. Following 3-4 push-pull perfusions with control artificial CSF of a site identified as reactive to 5-HT, ethanol in a concentration of 2.75 (471 mM) or 5.5 (942 mM) percent was perfused at the same locus over a 5-10 min interval. Successive samples of perfusate were assayed for their content of 5-HT by high performance liquid chromatography using electrochemical detection (HPLC-EC). Within a circumscribed region of the AH/POA of the rat maintained at an ambient temperature of 22 degrees C, ethanol induced either an immediate or delayed hypothermia of short latency or a transient decline followed by an immediate increase in core temperature. In each case, the shift in temperature depended on the anatomical site of push-pull perfusion. Overall, the fall in core temperature was accompanied by an inhibition in the efflux of 5-HT. However, the consequent rise in the rat's core temperature was associated with an enhanced release of 5-HT in the samples of perfusate collected from the AH/POA. These results suggest that serotonergic synapses within the AH/POA are apparently involved in the thermolytic effects of ethanol as well as in the thermogenesis following the interval of heat loss.
Department of Pharmacology, East Carolina University School of Medicine, Greenville, NC 27834.
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Huttunen
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Lapinlampi
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Myers
D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects*; D001931:Brain Mapping; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D011301:Preoptic Area / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000187:drug effects; D012701:Serotonin / Q000378:metabolism*
D011985:Receptors, Serotonin; D012701:Serotonin; D000431:Ethanol