The quantification of gamma-aminobutyric acid in the cerebrospinal fluid by a radioreceptorassay.
The development of a radioreceptor assay designed to measure gamma-aminobutyric acid (GABA) in CSF is described. The method is based on the presence of high affinity and selective GABA binding sites obtained from rat brain membrane preparations treated with 0.05% Triton X-100. The optimum protein concentration in the incubation medium, the duration of incubation to reach equilibrium, the temperature and the optimum pH are discussed. The standard curve permits measurements in the range 35 to 2250 nmol/l GABA. The imprecision of the method calculated from three different concentrations: 1125, 562 and 281 nmol/l shows coefficients of variation for 'within' and 'between' assays, between 5.2% and 9.3% and between 7.4% and 12.4%, respectively. The percentage of recovery is 102 +/- 3.3% (n = 4). This radioreceptorassay has a sensitivity of 14 nmol/l. The method is easy, rapid and not expensive. It enables analysis of small volumes of CSF (200 microliters). Several samples (greater than 20) can be analysed in the same run in about one hour.
Department of Pharmacology, U.C.L., Brussels, Belgium.
H
H
Mousah
P
P
Jacqmin
M
M
Lesne
D000818:Animals; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011869:Radioligand Assay / Q000379:methods; D051381:Rats; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000134:cerebrospinal fluid*
D005680:gamma-Aminobutyric Acid