Isoenzymes of the lung fluke Paragonimus uterobilateralis from Liberia.
The enzymes of 35 adult Paragonimus uterobilateralis were analysed using thin-layer starch gel electrophoresis. From a total of 21 enzyme systems studied, 15 proved to be useful for the description and recognition of this species. All individuals were identical concerning 11 enzymes. In four remaining enzymes, alanine aminotransferase (ALAT, hexokinase, aspartate aminotransferase and phosphogluconate dehydrogenase, two or three variants, also being partly typical for this species were observed. In a comparison involving seven different enzymes, there were no differences between the electrophoretic patterns of 35 adult and 24 juvenile P. uterobilateralis. Additional examinations of 30 adult P. uterobilateralis with isoelectric focusing on ultrathin-layer polyacrylamide gels revealed clearer separations of enzymes. The method showed corresponding results or identiy of all individuals tested with three representative enzymes (ALAT, glucosephosphate isomerase and adenylate kinase).
Department of Veterinary Medicine, Bernhard Nocht Institut of Nautical and Tropical Medicine, Hamburg, FRG.
U
U
Zillmann
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R
Sachs
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F
Ebert
D000410:Alanine Transaminase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D004592:Electrophoresis, Starch Gel; D006593:Hexokinase / Q000032:analysis; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D007988:Liberia; D010238:Paragonimus / Q000201:enzymology*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis
D007527:Isoenzymes; D010734:Phosphogluconate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D006593:Hexokinase