RNA polymerase II transcription factors H4TF-1 and H4TF-2 require metal to bind specific DNA sequences.
Specific DNA-binding and in vitro transcription activities of H4TF-1 and H4TF-2 are inactivated by chelating agents. Binding activity is restored by addition of Zn2+, and H4TF-2 is also reactivated by Fe2+. In contrast, preformed factor-DNA complexes are resistant to chelators. Therefore, metal ions are a required component of the H4TF-1 and H4TF-2 DNA-binding domains.
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
L
L
Dailey
S B
SB
Roberts
N
N
Heintz
D002413:Cations, Divalent; D002614:Chelating Agents / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D005296:Ferrous Compounds / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D010618:Phenanthrolines / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015032:Zinc / Q000494:pharmacology*
D002413:Cations, Divalent; D002614:Chelating Agents; D005296:Ferrous Compounds; D010618:Phenanthrolines; D014157:Transcription Factors; D004247:DNA; D012319:RNA Polymerase II; D015032:Zinc; C025205:1,10-phenanthroline