Major determinants of purine excretion from human lymphoblasts.
WI-L2 B lymphoblasts deficient in hypoxanthine-guanine phosphoribosyltransferase (HGPRT) excreted amounts of hypoxanthine two to three times larger than CEM T lymphoblasts deficient in HGPRT, despite similar growth rates. ATP consumption occurred at a higher rate in WI-L2 cells than in CEM cells when cultivated in a glucose-free buffer, because of higher RNA synthesis in WI-L2 cells. The introduction of actinomycin D and azaserine resulted in lower hypoxanthine excretion in WI-L2 cells than in CEM cells, not in parallel with changes of the adenylate pool size. When the energy charge was high, de novo purine synthesis was a major determinant for purine excretion. The adenylate pool ratio (AMP/ATP) change caused by the introduction of oligomycin was greater during ATP depletion and recovery in WI-L2 cells than in CEM cells. WI-L2 cells were observed to have AMP deaminase activity three to four times higher than CEM cells. The major component of AMP deaminase in these cells was liver type. The higher rate of RNA synthesis caused greater changes of (AMP/ATP) and required higher AMP deaminase activity for recovery. When the energy charge was low, AMP deaminase was a major determinant for purine excretion.
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
T
T
Iizasa
T
T
Miyamoto
D000659:AMP Deaminase / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D006801:Humans; D007042:Hypoxanthines / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D005561:Formates; D007042:Hypoxanthines; D007773:Lactates; D009711:Nucleotides; D011687:Purines; D000659:AMP Deaminase; D013936:Thymidine; D014529:Uridine