Interaction of the antitumor Au(I) complex [Au(Ph2P(CH2)2PPh2)2]Cl with human blood plasma, red cells, and lipoproteins: 31P and 1H NMR studies.
The bis-chelated tetrahedral gold(I) complex [Au(dppe)2]Cl, where dppe is Ph2P(CH2)2PPh2, is active in several animal tumor models. When added to human blood plasma in vitro it appears to bind to lipoproteins, giving a slightly broadened 31P NMR signal, and 1H NMR resonances which are too broad to detect. Some lipoprotein is denatured. 31P NMR studies suggest that some [Au(dppe)2]+ is transferred from plasma to red cells with a half-life of ca. 2 hr. The complex binds within red cell membranes and the 1H resonances of intracellular glutathione are unaffected. The 31P NMR resonance from [Au(dppe)2]+ in red cell membranes is observable only when the complex is mobilized by addition of sodium dodecyl sulphate, which also mobilizes membrane phospholipids.
Department of Chemistry, Birkbeck College, University of London, UK.
S J
SJ
Berners-Price
P J
PJ
Sadler
D000970:Antineoplastic Agents / Q000097:blood*; D011232:Chemical Precipitation; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006859:Hydrogen; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D050607:Organogold Compounds; D009942:Organometallic Compounds / Q000097:blood*; D009943:Organophosphorus Compounds / Q000097:blood*; D010758:Phosphorus; D011485:Protein Binding
D000970:Antineoplastic Agents; D008074:Lipoproteins; D050607:Organogold Compounds; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; D010758:Phosphorus; C050185:bis(1,2-bis(diphenylphosphino)ethane)gold(I); D006859:Hydrogen