Electroelution of DNA and protein from polyacrylamide and agarose gels.
An electroelution method is described for the recovery of DNA and protein from agarose or polyacrylamide gels. The samples to be electroeluted are compartmentalized in a modified microcentrifuge tube fitted with dialysis membranes. This procedure is simple, rapid, inexpensive and efficient. Within 30 min to 2 hrs, the recovery of the sample is nearly quantitative. DNA fragments recovered can be directly subjected to DNA sequence analysis or enzymatic reactions after ethanol precipitation. Proteins can also be recovered after separation by acrylamide gel in the presence or absence of detergents and be ready for further analysis.
Institute of Biochemical Sciences, National Taiwan University, Taipei, Republic of China.
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D000818:Animals; D002417:Cattle; D004247:DNA / Q000302:isolation & purification*; D004563:Electrochemistry; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D013552:Swine
D011506:Proteins; D012710:Serum Albumin, Bovine; D013006:Growth Hormone; D004247:DNA