High-performance liquid chromatographic procedure for quantitative determination of pentylenetetrazol in serum and discrete areas of rat brain.
A precise and reproducible high-performance liquid chromatographic method for the determination of pentylenetetrazol in serum and brain tissue is described. The procedure employs reversed-phase chromatography, monitoring the eluant at 202 nm. Quantification is based on peak-height ratio of the drug to the internal standard (p-methylphenobarbital). A linear response is obtained to 100 micrograms/ml (serum) or micrograms/g (brain tissue). Within-day and between-day precision are smaller than 5%, and analytical recovery is greater than 95%. Numerous drugs tested do not interfere with the assay. The method has been used to investigate the kinetics of pentylenetetrazol distribution in serum and in discrete areas of rat brain.
Soto-Otero
R
R
University of Santiago, Department of Biochemistry and Molecular Biology, School of Medicine, Spain.
Mendez-Alvarez
E
E
Sierra-Paredes
G
G
Galan-Valiente
J
J
Aguilar-Veiga
E
E
Sierra-Marcuño
G
G
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D010433:Pentylenetetrazole / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains
D007202:Indicators and Reagents; D010433:Pentylenetetrazole