Ketimine rings: useful detectors of enzymatic activities in solution and on polyacrylamide gel. Detection of L-amino acid oxidase, glutamine transaminase, pantetheinase, and acylase I.
A simple procedure has been developed for the detection of L-amino acid oxidase, glutamine transaminase, pantetheinase, and acylase I in solution and on polyacrylamide gels. The method is based on the strong absorbance at 296 nm of some ketimine rings which can be directly produced by the enzymatic reaction or formed by the reaction of the enzymatic product with 3-bromopyruvate. The procedure allows one to visualize up to about 1-10 mU of enzyme.
Ricci
G
G
Institute of Biochemical Sciences, University of Chieti, Rome, Italy.
Caccuri
A M
AM
Lo Bello
M
M
Solinas
S P
SP
Nardini
M
M
D000581:Amidohydrolases / Q000032:analysis*; D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000603:Amino Acids, Sulfur; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D058851:GPI-Linked Proteins; D007202:Indicators and Reagents; D050830:L-Amino Acid Oxidase; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D000637:Transaminases / Q000032:analysis*
D000603:Amino Acids, Sulfur; D058851:GPI-Linked Proteins; D007202:Indicators and Reagents; D012996:Solutions; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D000637:Transaminases; C020656:glutamine-pyruvate aminotransferase; D000581:Amidohydrolases; C022314:pantetheinase; C019672:aminoacylase I