The leading sequence of caulimovirus large RNA can be folded into a large stem-loop structure.
The 600 nt long sequences preceeding the first large ORFs (ORF VII) of three caulimoviruses, although varying in primary sequence, can be folded into a large stem/loop structure centered around a conserved stretch of 36 nucleotides. Deletions of the conserved sequence delay symptom appearance considerably, but do not affect expression of a reporter gene in plant protoplasts. Another striking similarity between the leaders concerns the number and distribution of small open reading frames (sORF) they carry. Expression of two of these sORFs was tested by fusion of a reporter gene: both were expressed in plant protoplasts.
Friedrich Miescher-Institut, Basel, Switzerland.
J
J
Fütterer
K
K
Gordon
J M
JM
Bonneville
H
H
Sanfaçon
B
B
Pisan
J
J
Penswick
T
T
Hohn
D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D005814:Genes, Viral; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics* / Q000648:ultrastructure; D012190:Retroviridae / Q000235:genetics; D012984:Software
D012367:RNA, Viral