Primary structure and mRNA localization of protein F1, a growth-related protein kinase C substrate associated with synaptic plasticity.
Protein F1 is a neuron-specific, synaptic-enriched, membrane-bound substrate of protein kinase C (PKC) whose phosphorylation is related to synaptic plasticity in the adult. The sequence of 26 N-terminal amino acids was determined from purified rat protein F1. A 78-mer synthetic oligonucleotide designed from the partial N-terminal sequence enabled identification of protein F1 cDNA clones in a rat brain library. F1 protein is a 226 amino acid protein encoded by a 1.5 kb brain-specific, developmentally-regulated mRNA. Transcripts for protein F1 can be detected at birth, and their level declines after maturation. A full-length cDNA clone was transcribed and translated in vitro. Translation products could be immunoprecipitated with anti-F1 antibodies. In situ hybridization analysis revealed protein F1 transcripts in hippocampal pyramidal cells, but not in granule cells. In cerebellum, granule cells contained protein F1 mRNA, while Purkinje cells did not. Co-localization of protein F1 with protein kinase C-II [PKC-II (beta)], rather than PKC-I (gamma) suggests that PKC-II may phosphorylate protein F1.
Rosenthal
A
A
Genentech, Inc., Department of Molecular Biology, South San Francisco, CA 94080.
Chan
S Y
SY
Henzel
W
W
Haskell
C
C
Kuang
W J
WJ
Chen
E
E
Wilcox
J N
JN
Ullrich
A
A
Goeddel
D V
DV
Routtenberg
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D019922:GAP-43 Protein; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology; D009473:Neuronal Plasticity; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins / Q000235:genetics* / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000502:physiology*; D014158:Transcription, Genetic
D019922:GAP-43 Protein; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D004247:DNA; D011493:Protein Kinase C