DDAVP and epinephrine-induced changes in the localization of von Willebrand factor antigen in endothelial cells of human oral mucosa.
A peroxidase-labeled antibody technique revealed von Willebrand factor antigen (vWF:Ag) in rough endoplasmic reticulum (rER), the perinuclear region, and the cytoplasmic vesicles of capillary endothelial cells in oral mucosa. After administration of epinephrine or 1-deamino-8-D-arginine vasopressin (DDAVP), the localization of vWF:Ag was shown to have changed to the basement membrane and the surrounding interstitium. This change of vWF:Ag localization induced by epinephrine and DDAVP may play a role in the adhesion of platelets to subendothelium following endothelial injury during surgery and may be an unknown hemostatic effect of these drugs.
Department of Oral Surgery, Nagoya University School of Medicine, Japan.
M
M
Takeuchi
H
H
Nagura
T
T
Kaneda
D000328:Adult; D000941:Antigens / Q000032:analysis*; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D009061:Mouth Mucosa / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D014841:von Willebrand Factor / Q000276:immunology
D000941:Antigens; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; D003894:Deamino Arginine Vasopressin; D004837:Epinephrine