A modified spectrofluorometric assay for DNA using adriamycin.
A spectrofluorometric assay for DNA is described. The changes in the fluorescence of adriamycin when it binds to DNA may be quantitated in terms of the amount of DNA present. The assay is specific and reproducible, being free from interference from RNA, protein, and sample turbidity.
Kennedy
D G
DG
Department of Biochemistry, Queen's University of Belfast, Northern Ireland, United Kingdom.
Nelson
J
J
van den Berg
H W
HW
Murphy
R F
RF
D001943:Breast Neoplasms / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D004317:Doxorubicin; D006801:Humans; D011402:Pronase / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured / Q000032:analysis
D004273:DNA, Neoplasm; D004317:Doxorubicin; D004247:DNA; D011402:Pronase