Relationship between reestablishment of sarcolemma-glycocalyx ultrastructures and restoration of transmembrane potentials in cultured rat heart cells.
Simultaneous studies on sarcolemma-glycocalyx ultrastructures and electrophysiological properties of the trypsin-released rat heart cells were carried out in order to define the relationship between sarcolemmal membrane repair and transmembrane potential recovery in the long-term cultured heart cells. Based on electron microscopic observations of the trypsinized heart cells maintained in the long-term culture, serial alterations of sarcolemma-glycocalyx complex could be divided into three successive stages. A defective stage of the sarcolemma-glycocalyx complex was present in the cultured cells between day 3 and day 6 of incubation. A repaired stage of the sarcolemma-glycocalyx complex was observed in the cells from day 7 to day 9 of incubation. A well-organized stage of the sarcolemma-glycocalyx complex was seen in the cells after ten days of incubation. Sequential measurements of electrophysiological parameters of the cultured heart cells showed a diphasic evolution of maximum diastolic potential and action potential amplitude, with an initial decrease from day 3 to day 9 of incubation and a later return to normal range after ten days of incubation. There seemed a good correlation between electrophysiological properties and sarcolemma-glycocalyx ultrastructures. Thus, we conclude that restoration of the electrophysiological properties of the cultured cells is closely related to the reorganization of the defective sarcolemmal membrane.
Lee
Y S
YS
Department of Internal Medicine, Chang Gung Memorial Hospital, Taipei, Taiwan, R.O.C.
Hsu
T S
TS
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D006023:Glycoproteins / Q000032:analysis*; D006321:Heart / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure*; D011134:Polysaccharides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012508:Sarcolemma / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D014357:Trypsin
D006023:Glycoproteins; D011134:Polysaccharides; D014357:Trypsin