Multiple elements are required for expression of an intermediate filament gene.
The expression of vimentin is unique within the intermediate filament multigene family. It is the only member which deviates from its usual tissue-specific expression pattern and whose 5'-flanking region contains multiple GC boxes, the binding site for Sp1. The activity of vimentin 5'-end:CAT fusions has been compared in cells where vimentin is highly expressed (mouse L cells) or not expressed at all (MH1C1). In addition, CAT activity has been examined by microinjection into Xenopus oocytes. Both in vivo expression and in vitro binding studies implicate Sp1 as a general regulatory factor in vimentin gene expression. Increased expression of 5'-end:CAT fusions in mouse L cells suggests that a fibroblast-specific enhancer element resides in the region -321 to -160. Low transcriptional activity in MH1C1 cells may be due to either the lack of this positive transcription factor(s) or the presence of a repressor element. Here, we demonstrate that the unique and complex pattern of vimentin gene expression is controlled by multiple cis-acting elements.
Sax
C M
CM
Department of Human Genetics, Massey Cancer Center, Richmond, VA 23298.
Farrell
F X
FX
Tobian
J A
JA
Zehner
Z E
ZE
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002846:Chromatography, Affinity; D003599:Cytoskeleton / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification; D005786:Gene Expression Regulation; D007382:Intermediate Filaments / Q000378:metabolism*; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D014158:Transcription, Genetic; D014746:Vimentin / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D004268:DNA-Binding Proteins; D014746:Vimentin