Neuronal differentiation of mouse neural crest cells in vitro.
The purpose of the present study is to analyze the effect of serum or chick embryo extract (CEE) on the neuronal differentiation of the mouse neural crest cells. When the crest cells were cultured in the medium containing serum at low concentration (5% calf serum), neurite outgrowth was observed. The active outgrowth was detected at 3-4 days in culture. However, in the medium supplemented with 20% calf serum, no neurite appeared, and the crest cells remained fibroblast-like. The differentiation of adrenergic neurons was observed when the crest cells were cultured in the medium containing CEE along with serum.
Ito
K
K
Biological Institute, Tohoku University, Sendai, Japan.
Morita
T
T
Takeuchi
T
T
D000818:Animals; D001790:Blood Physiological Phenomena; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D009432:Neural Crest / Q000166:cytology* / Q000187:drug effects; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure*; D014020:Tissue Extracts / Q000494:pharmacology
D003470:Culture Media; D014020:Tissue Extracts