The stem-loop structure at the 3' end of histone mRNA is necessary and sufficient for regulation of histone mRNA stability.
Chimeric genes were made by fusing mouse histone genes with a human alpha-globin gene. The genes were introduced into mouse L cells and the stability of the chimeric mRNAs was measured when DNA synthesis was inhibited. An mRNA containing all the globin coding sequences and the last 30 nucleotides of the histone mRNA was degraded at the same rate as histone mRNA.
Pandey
N B
NB
Department of Chemistry, Florida State University, Tallahassee 32306.
Marzluff
W F
WF
D000818:Animals; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000096:biosynthesis; D005914:Globins / Q000235:genetics; D006657:Histones / Q000235:genetics*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007438:Introns; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection
D003062:Codon; D006657:Histones; D012333:RNA, Messenger; D005914:Globins; D004247:DNA; D006918:Hydroxyurea