Fluorescence-activated cell sorting of hybrid and transfected cells.
Mouse cells containing human surface antigen genes introduced by cell hybridization or DNA transfection can be labeled by indirect immunofluorescence and isolated using the FACS. As illustrated in this chapter, this methodology facilitates genetic studies of the human cell surface ranging from the initial chromosome mapping of a surface antigen gene to its isolation and cloning.
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D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods; D005796:Genes; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology*; D007739:L Cells / Q000166:cytology; D008285:Major Histocompatibility Complex; D051379:Mice; D010957:Plasmids; D014162:Transfection
D006680:HLA Antigens; D004247:DNA