[Interaction of human albumin with sorbents containing immobilized dyes].
Interaction of human albumin with immobilized dye-adsorbents was investigated by frontal chromatographic analysis and static adsorption. The relative strength of the interaction is diminished in the following order: cibacron blue F3GA, orange 4K, claret CT, orange 5K, red-brown 2KT, light resistant yellow 2KT, bright blue KX, scarlet 2Zh, bright red 6C, bright yellow 53, red-brown 2K, golden yellow 2KX, scarlet 4ZhT and yellow 2KT. Following this order the absorbents can be used for purification of human albumin from non-specific impurities taking into account stronger adsorption of albumin or impurities. Adsorbents with dyes immobilized on macroporous silica were found to be promising for large-scale purification of human albumin. The silica adsorbent containing red brown 2K is effective for the removal of the following impurities from placental albumin: chorionic gonadotropin, alpha-fetoprotein and placental lactogen.
Bashkevichiute
B B
BB
Sudzhiuvene
O F
OF
Pesliakas
I I
II
Glemzha
A A
AA
Migunov
V N
VN
D001665:Binding Sites; D002847:Chromatography, Agarose; D004396:Coloring Agents / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism*; D014227:Triazines / Q000378:metabolism
D004396:Coloring Agents; D012709:Serum Albumin; D014227:Triazines; C012842:Cibacron Blue F 3GA
Issledovanie vzaimodeĭstviia chelovecheskogo al'bumina s sorbentami, soderzhashchimi immobilizovannye krasiteli.