Are sheep and buffalo prolactins sulfated?
Radioactive sulfate (35SO2-4) has been shown to be incorporated into immunoprecipitable prolactin-like material from incubated minces of sheep and buffalo pituitaries. The 35S-labelled prolactin could be purified by standard procedures. On SDS-PAGE, the 35S-labelled prolactin rich fraction gives two major Coomassie blue bands around 25KDa and these on Western blot analysis gave positive bands. Radioactive [14C]- mannose was also found incorporated into the prolactin like material. The nature of sulphate link to the peptide is not known. It could be sugar-SO4 and/or Tyrosine-SO4.
Department of Zoology, University of Delhi.
R
R
Kohli
N
N
Chadha
K
K
Muralidhar
D000818:Animals; D002020:Buffaloes; D006031:Glycosylation; D008970:Molecular Weight; D010902:Pituitary Gland / Q000032:analysis; D011388:Prolactin / Q000032:analysis*; D012756:Sheep; D013045:Species Specificity; D013431:Sulfates / Q000032:analysis*
D013431:Sulfates; D011388:Prolactin