Liquid chromatographic determination of sulfamoyldapsone in swine tissues.
A liquid chromatographic (LC) method is described for the quantitative determination of sulfamoyldapsone (2-sulfamoyl-4,4'-diaminodiphenyl sulfone) in swine muscle, liver, kidney, and fat. Sulfamoyldapsone was extracted from tissues with acetonitrile saturated with n-hexane. The extract was washed with n-hexane saturated with acetonitrile, concentrated, and cleaned up by alumina column chromatography. Sulfamoyldapsone was separated on an ODS column by using acetonitrile-methanol-water (6 + 18 + 76) and was detected at 292 nm. Overall average recovery of sulfamoyldapsone added to tissues at levels of 0.1 and 0.5 microgram/g was 93.3% +/- 6.0. Detection limit was 0.02 microgram/g in these tissues.
Ministry of Agriculture, Forestry and Fisheries, National Veterinary Assay Laboratory, Tokyo, Japan.
Y S
YS
Endoh
R
R
Yamaoka
N
N
Sasaki
D000273:Adipose Tissue / Q000032:analysis; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D003622:Dapsone / Q000031:analogs & derivatives* / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008460:Meat / Q000032:analysis*; D013552:Swine
D000890:Anti-Infective Agents; C003361:SDDS; D003622:Dapsone