Platelet adhesion onto polyether-urethane urea derivatives: effect of cytoskeleton proteins of the platelet.
Adhesion and activation of platelets upon adhesion onto synthetic polymers were investigated with reference to participation of the cytoskeleton proteins. Platelets were treated with cytoskeleton breakers, and then the adhesion of platelets onto polyetherurethane urea derivatives and serotonin release from adhered platelets were investigated. In the adhesion onto glass, platelets were strongly stimulated and accompanied rearrangement of the cytoskeleton system, and also serotonin release involved the action of the cytoskeleton system. On the other hand, platelets were not strongly stimulated upon adhering onto polyetherurethane urea derivatives. The platelet adhesion onto cationic polymers exceptionally accompanied the rearrangement of the cytoskeleton system. The participation of the cytoskeleton in platelet adhesion onto polyetherurethane urea derivatives was influenced by the presence of plasma proteins. It was found that protein layers deposited on the material surface play an important role in platelet adhesion.
Ito
Y
Y
Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
Imanishi
Y
Y
Sisido
M
M
D001792:Blood Platelets / Q000502:physiology*; D003598:Cytoskeletal Proteins / Q000097:blood* / Q000502:physiology; D005898:Glass; D006801:Humans; D010973:Platelet Adhesiveness; D011140:Polyurethanes; D012701:Serotonin / Q000097:blood; D013329:Structure-Activity Relationship
D003598:Cytoskeletal Proteins; D011140:Polyurethanes; D012701:Serotonin; C044690:polyetherurethane urea