[Fertilization in vitro. Perspectives for improving the technics].
Techniques of embryo cultures at an early stage, in spite of all the progress in that field, still present a major problem from the viability standpoint. The egg interrupts its segmentation, or, if it develops with an apparently normal chronology, the viability after transfer in vivo is very poor. Since a few years, trials in co-culture have been attempted. Culture on fibroblastic sheets gave disappointing results. As far as we are concerned, we have followed two different approaches: culture within the oviduct explanted in vitro and co-culture in the presence of embryonic tissue. The two types of co-culture give promising results, but it seems that the future lies in cultures on tubal epithelial cells. It will be necessary to obtain cellular lineage of these epithelia and to define culture media corresponding to the needs of the embryo as well as of these cells, since the embryo is an autonomous system, extremely sensitive to media alterations.
Laboratoire de Biologie, Laboratoire associé à l'INRA-INSA, Villeurbanne.
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Menezo
D000818:Animals; D005307:Fertilization in Vitro / Q000379:methods*; D005544:Forecasting; D006801:Humans
Fécondation in vitro. Perspectives d'amélioration des techniques.