An immunocytochemical method for studying embryo cytokinetics.
The immunocytochemical detection by a monoclonal antibody of bromodeoxyuridine incorporated into S-phase cells allows the identification of proliferating cells. In this study we demonstrate that the labelling of embryo tissues is achieved by a single administration of BrdUrd to the mother after a 1 hour-labelling period. This simple and rapid technique facilitates the detection of proliferating cells within the embryo for the study of developing tissues and embryo cytokinetics.
Casasco
A
A
Istituto di Istologia ed Embriologia generale, Università di Pavia, Italy.
Calligaro
A
A
Casasco
E
E
Marchetti
C
C
Poggi
P
P
Casasco
M
M
D000818:Animals; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D001973:Bromodeoxyuridine