Human mitochondrial aldehyde dehydrogenase: mRNA expression in different tissues using a specific probe isolated from a cDNA expression library.
Using monospecific antibodies against mitochondrial aldehyde dehydrogenase (ALDH I), cDNA clones were isolated from a human liver cDNA library constructed in the plasmid expression vector pEX. Two overlapping clones pEXAL21 and pEXAL43 containing inserts of 861 bp and 1180 bp respectively, code for the carboxy-terminal end of ALDH I as confirmed by sequence analysis. The messenger RNA of ALDH I was estimated by Northern blot analysis to contain approximately 2350 nucleotides. RNA hybridizations revealed that the ALDH I gene transcription is generally low in fetal tissues. Especially, transcription in the adult liver is up to 5-fold higher than in the fetal liver.
Institute of Human Genetics, University of Hamburg, FRG.
T
T
Braun
E
E
Bober
J
J
Schaper
D P
DP
Agarwal
S
S
Singh
H W
HW
Goedde
D000328:Adult; D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005333:Fetus / Q000378:metabolism; D006736:Horses; D006801:Humans; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA; D000444:Aldehyde Dehydrogenase