Metabolic behaviour of a stable DNA-protein complex.
1. A stable DNA-protein complex resisting all treatments dissociating noncovalently associated proteins was isolated from mouse erythroleukemia cells. 2. Two-dimensional tryptic peptide mapping of this DNA-linked protein component revealed a remarkable similarity to the maps of the corresponding proteins from other mammalian chromatins. 3. Labelling experiments showed that the protein component was metabolically stable.
Avramova
Z
Z
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Mikhailov
I
I
Tsanev
R
R
D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping; D014316:Tritium
D002250:Carbon Radioisotopes; D004273:DNA, Neoplasm; D003852:Deoxyribonucleoproteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D014316:Tritium