Simple method for derivatization of monoglycerides and diglycerides.
A method has been developed for derivatization of monoglycerides and diglycerides to their propyl esters by using propionic anhydride in the presence of pyridine. The derivatives are volatile and thus can be separated and identified by gas chromatography (GC). Completion of the reaction is monitored by thin-layer chromatography. Monoglyceride propyl esters were positively identified by gas chromatography and by gas chromatography/mass spectrometry. No side products were observed in the derivatization reaction which requires 35 min at 75 degrees C. GC analysis of the mono- and diglyceride propyl esters can be completed in approximately 30 min.
Ross Laboratories, Analytical Research and Services, Columbus, OH 43216.
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Lee
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Hastilow
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Smith
D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004075:Diglycerides / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005989:Glycerides / Q000032:analysis*; D007202:Indicators and Reagents
D004075:Diglycerides; D005989:Glycerides; D007202:Indicators and Reagents