The biosynthesis of steroid glucuronides in the testis of the zebrafish, Brachydanio rerio, and their pheromonal function as ovulation inducers.
In female zebrafish ovulation could be induced by male holding water, testis homogenates, and testis fractions containing steroid glucuronides. Deglucuronidation of these fractions led to a loss of ovulation-inducing potency, indicating steroid glucuronides as ovulation inducers. The chemical substances were perceived by the recipient females by means of olfaction. Incubation experiments showed the capacity of the testes to synthesize various C19 and C21 steroids and seven different steroid glucuronides, i.e., 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one-, testosterone-, androsterone-, epiandrosterone-, 5 alpha-androstane-3 alpha, 17 beta-diol-, and 5 alpha-androstane-3 beta, 17 beta-diol glucuronide. GC-MS analysis showed the presence of glucuronides of 5 alpha-androstane-3 alpha, 17 beta-diol and cholesterol in male holding water, the latter probably originating from the liver. These compounds may be among the steroid glucuronides functioning as ovulation-inducing pheromones.
Department of Experimental Zoology, University of Utrecht, The Netherlands.
R
R
van den Hurk
W G
WG
Schoonen
G A
GA
van Zoelen
J G
JG
Lambert
D000735:Androstenedione / Q000494:pharmacology; D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D010060:Ovulation; D010675:Pheromones; D011284:Pregnenolone / Q000494:pharmacology; D011372:Progestins / Q000096:biosynthesis*; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D014867:Water / Q000032:analysis / Q000494:pharmacology; D015027:Zebrafish
D012739:Gonadal Steroid Hormones; D010675:Pheromones; D011372:Progestins; D014867:Water; D000735:Androstenedione; D011284:Pregnenolone