Assessment of intestinal damage in rotavirus infected neonatal mice by a D-xylose absorption test.
A D-xylose absorption test has been standardized for use in newborn mice. It was used to measure small intestine dysfunction in neonate mice following infection with various isolates of rotaviruses. A xylose dose of 1 mg/g body weight was required to produce a maximum level of 100 micrograms/100 microliters of plasma 2 h after administration of D-xylose. The mice inoculated with rotavirus absorbed significantly less D-xylose compared with uninoculated control mice. A micromethod is described which proved to be suitable to quantitate D-xylose for determination of absorptive function of the small intestine in small animals such as newborn mice.
Veterinary Infectious Disease Organization (VIDO), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
M K
MK
Ijaz
M I
MI
Sabara
P J
PJ
Frenchick
L A
LA
Babiuk
D000818:Animals; D007408:Intestinal Absorption; D007410:Intestinal Diseases / Q000382:microbiology* / Q000503:physiopathology; D051379:Mice; D012016:Reference Values; D012400:Rotavirus Infections / Q000503:physiopathology*; D013045:Species Specificity; D014994:Xylose / Q000378:metabolism*
D014994:Xylose