Nucleotide sequence for the cDNA of the bovine beta B2 crystallin and assignment of the orthologous human locus to chromosome 22.
We have identified and characterized two over-lapping bovine cDNA clones corresponding to the bovine crystallin beta Bp. The longer of the two clones, which contains the entire coding and 3' untranslated region as well as 54 nucleotides of the 5' untranslated sequence was used to identify and map an orthologous human gene, Hu beta B2, to chromosome 22, q11.2-q12.2. As one other human beta-crystallin, Hu beta A3/A1, has been mapped to chromosome 17, our results indicate that, unlike the tightly linked gamma-crystallins, the human beta-crystallins are not syntenic within the genome.
Mount Sinai Research Institute, Toronto, Ontario, Canada.
D
D
Hogg
M B
MB
Gorin
C
C
Heinzmann
S
S
Zollman
T
T
Mohandas
I
I
Klisak
R S
RS
Sparkes
M
M
Breitman
L C
LC
Tsui
J
J
Horwitz
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003459:Crystallins / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010449:Peptide Mapping
D003459:Crystallins; D004247:DNA