Four classes of mRNA are expressed from the mouse int-2 gene, a member of the FGF gene family.
Mouse embryos at 7.5 days of gestation and endodermal cells derived from embryonal carcinoma cells each express four int-2 mRNAs of similar size and relative abundance. To determine their structure and coding potential, we prepared a cDNA library from endoderm mRNA and isolated several int-2 cDNAs. Structural analysis of these cDNAs combined with Northern blot hybridization and primer extension analyses of int-2 mRNA revealed that the differences between the mRNAs are generated through the use of two alternate transcriptional start sites and two alternate polyadenylation sites. All four mRNAs share a common core sequence that encodes a protein with amino acid similarity to fibroblast growth factor.
Mansour
S L
SL
Department of Anatomy, University of California, San Francisco 94143.
Martin
G R
GR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004707:Endoderm / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000235:genetics*; D005796:Genes; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D013724:Teratoma; D014158:Transcription, Genetic
D012333:RNA, Messenger; D005346:Fibroblast Growth Factors; D004247:DNA