Characterization of the products generated from oversulphated dermatan sulphate isomers with chondroitinase-B by high-performance liquid chromatography.
Characterization with specific chondroitinase-B of the constituents of the unsaturated sulphated disaccharides generated from variously sulphated dermatan sulphate (DS) isomers in human kidney tissues was carried out by high-performance liquid chromatography, using a sulphonized styrene-divinylbenzene copolymer. The products derived from the variously sulphated DS isomers with the chondroitinase-B at specific unsaturated disaccharides were characterized as unsaturated mono-, di- and trisulphated disaccharides. The results suggest that chondroitinase-B converts DS isomers into unsaturated 4-sulphated disaccharide, unsaturated disulphated disaccharides types B and H (delta Di-diSB and delta Di-diSH) and unsaturated trisulphated disaccharide (delta Di-triS), which possess sulphate linkage (s) at position 4 of the galactosamine residues and, in addition, at position 6 of galactosamine and/or position 2 of iduronic acid. The delta Di-diSB, delta Di-diSH and delta Di-triS were found for the first time in human kidney tissue.
Murata
K
K
Department of Medicine and Physical Therapy, University of Tokyo, School of Medicine, Japan.
Yokoyama
Y
Y
D002807:Chondroitin / Q000031:analogs & derivatives*; D002808:Chondroitin Lyases; D002810:Chondroitinases and Chondroitin Lyases; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003871:Dermatan Sulfate / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007536:Isomerism; D007668:Kidney / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D003871:Dermatan Sulfate; D002807:Chondroitin; D002808:Chondroitin Lyases; D002810:Chondroitinases and Chondroitin Lyases