Subunit III of bovine procarboxypeptidase A: its relationship to human pancreatic elastase 1.
This paper is a continuation of our study of various animal pancreatic enzymes which are related to human pancreatic elastase 1 (Sziegoleit, A. & Linder, D. (1986) Biol. Chem. Hoppe-Seyler, 367, 527-531). The isolation and immunological analysis of the related protein from bovine pancreas disclosed that the third subunit of the procarboxypeptidase A complex is the antibody-binding component. The similarity of this subunit to elastase 1 is affirmed by comparison of their primary structures. While the complete amino-acid sequence of bovine subunit III recently has been published (Venot, N., Sciaky, M., Puigserver, A., Desnuelle, P. & Laurent, G. (1986) Eur. J. Biochem. 157, 91-99), we here present the amino-acid sequence of the carboxy-terminal tryptic peptide of human pancreatic elastase 1 showing a high degree of homology.
Sziegoleit
A
A
Institut für Medizinische Mikrobiologie, Universität Giessen.
Krack
P
P
Wendorf
P
P
Linder
D
D
D000818:Animals; D002268:Carboxypeptidases / Q000032:analysis*; D043422:Carboxypeptidases A; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D007525:Isoelectric Focusing; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000032:analysis*; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate
D004792:Enzyme Precursors; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; C048139:Coomassie blue; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D010196:Pancreatic Elastase