DDAVP and epinephrine-induced changes in the localization of von Willebrand factor antigen in endothelial cells of human oral mucosa.
A peroxidase-labeled antibody technique revealed von Willebrand factor antigen (vWF:Ag) in rough endoplasmic reticulum (rER), the perinuclear region, and the cytoplasmic vesicles of capillary endothelial cells in oral mucosa. After administration of epinephrine or 1-deamino-8-D-arginine vasopressin (DDAVP), the localization of vWF:Ag was shown to have changed to the basement membrane and the surrounding interstitium. This change of vWF:Ag localization induced by epinephrine and DDAVP may play a role in the adhesion of platelets to subendothelium following endothelial injury during surgery and may be an unknown hemostatic effect of these drugs.
Takeuchi
M
M
Department of Oral Surgery, Nagoya University School of Medicine, Japan.
Nagura
H
H
Kaneda
T
T
D000328:Adult; D000941:Antigens / Q000032:analysis*; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D009061:Mouth Mucosa / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D014841:von Willebrand Factor / Q000276:immunology
D000941:Antigens; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; D003894:Deamino Arginine Vasopressin; D004837:Epinephrine