Molecular diagnosis of haematological disorders using DNA from stored bone marrow slides.
A method is described for the extraction of high molecular weight DNA from bone marrow slides which have been stored at room temperature for more than 10 years. This technique widens the scope of molecular analysis of haematological malignancies to include the investigation of cases where the original malignant cells are not otherwise available.
Department of Haematology, John Radcliffe Hospital, Oxford.
M F
MF
Fey
S P
SP
Pilkington
C
C
Summers
J S
JS
Wainscoat
D001853:Bone Marrow / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D007938:Leukemia / Q000175:diagnosis*; D008970:Molecular Weight; D013997:Time Factors; D014021:Tissue Preservation
D004273:DNA, Neoplasm