High-performance liquid chromatography of underivatized gangliosides.
High-performance liquid chromatographic systems have been developed which use DEAE-Iatrobeads and Aquasil SS or amino-silica gel to attain fine separation of gangliosides in a short time. Two UV-transparent solvent systems containing potassium chloride or tetramethylammonium chloride were devised and optimized, especially for the separation of polysialogangliosides.
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
S
S
Ando
H
H
Waki
K
K
Kon
D000818:Animals; D001923:Brain Chemistry; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D005732:Gangliosides / Q000302:isolation & purification*; D008252:Macaca fascicularis; D013056:Spectrophotometry, Ultraviolet
D005732:Gangliosides