Lactate dehydrogenase in rat mitochondria.
Small but persistent amounts of L-lactate dehydrogenase (LDH) activity were found in mitochondrial preparations isolated from rat heart, kidney, liver, and lymphocytes. Brain mitochondrial preparations were also isolated, but the results were inconclusive. A variety of cytosolic markers were used and it was found that essentially no cytosolic contamination was present except in brain preparations. A bacterial protease was used along with digitonin fractionation to determine localization of the mitochondrial LDH. Approximately 80% of the LDH activity associated with heart and kidney mitochondrial preparations was on the inside compared to about 40% for liver. Lymphocyte mitochondrial LDH activity was about 70% on the inside. Cytosolic LDH-5 preferentially adheres to outer mitochondrial membrane of liver, kidney, and heart. Agarose gel electrophoresis showed LDH isozymes in mitochondria qualitatively similar to that of the corresponding cytosol except in kidney mitochondrial preparations, where a specific electrophoretic band was found which did not correspond to any of the common LDH isozymes.
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia/Virginia Commonwealth University, Richmond 23298.
R B
RB
Brandt
J E
JE
Laux
S E
SE
Spainhour
E S
ES
Kline
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000648:ultrastructure; D002458:Cell Fractionation; D003600:Cytosol / Q000201:enzymology; D004072:Digitonin / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D007527:Isoenzymes / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology / Q000648:ultrastructure; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008214:Lymphocytes / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D008929:Mitochondria, Heart / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D004072:Digitonin