On chromosomal DNA modifications by chemical and physical treatment of C-bands.
Experiments were performed on fixed metaphase chromosomes using standard techniques for revealing paracentromeric heterochromatin (C bands) followed by staining with acridine orange with the aim of studying C-banding mechanism. Data obtained suggest that the specific resistance to the chemical-physical treatments of the heterochromatic areas is a consequence of the particular structural conditions that the C-positive material shows only after its early renaturation.
Centro di Citomorfologia Umana C.N.R., Chieti, Italy.
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Stuppia
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Calabrese
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Guanciali Franchi
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Ferrucci
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Palka
D000165:Acridine Orange; D001464:Barium / Q000494:pharmacology*; D017609:Barium Compounds; D002498:Centrifugation; D002871:Chromosome Banding; D006570:Heterochromatin / Q000187:drug effects*; D006801:Humans
D017609:Barium Compounds; D006570:Heterochromatin; C012766:barium hydroxide; D001464:Barium; D000165:Acridine Orange