Effect of disulfiram on the pre-steady-state burst in the reactions of sheep liver cytoplasmic aldehyde dehydrogenase.
Stopped-flow spectrophotometric experiments show that modification by disulfiram not only lowers the steady-state rates but also decreases the size of bursts seen in both dehydrogenase and esterase reactions catalysed by sheep liver cytoplasmic aldehyde dehydrogenase. This observation is consistent with the proposal that a catalytically essential group is modified by disulfiram and that this group mediates both dehydrogenase and esterase activities.
Kitson
T M
TM
Department of Biochemistry, University of Hull, U.K.
D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000818:Animals; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D004221:Disulfiram / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004950:Esterases / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D012756:Sheep; D013053:Spectrophotometry
D000444:Aldehyde Dehydrogenase; D004950:Esterases; D004221:Disulfiram