Use of mixed perfluorinated ion-pairing agents as solvents in ion-pair high-performance liquid chromatography for the preparative purification of aminoglycoside antibiotics.
The possibility of using mixed ion-pair reversed-phase high-performance liquid chromatography for the preparative purification of aminoglycoside antibiotics was investigated. Retention can be satisfactorily defined in aqueous conditions but efficiency is critical. The analysis of a polynominal model allowed ion-pairing agents compositions to be designed leading to improved efficiency. Inexpensive aqueous mobile phases using volatile buffers can be defined for the satisfactory ion-pair preparative chromatography of aminoglycosides.
Centre de Transfert en Biotechnologie-Microbiologie (INSA), Toulouse, France.
I
I
De Miguel
E
E
Puech-Costes
D
D
Samain
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005466:Fluorocarbons; D007202:Indicators and Reagents; D012997:Solvents
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005466:Fluorocarbons; D007202:Indicators and Reagents; D012997:Solvents