Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.
A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.
Lippke
J A
JA
BioTechnica Diagnostics, Cambridge, Massachusetts 02138.
Strzempko
M N
MN
Raia
F F
FF
Simon
S L
SL
French
C K
CK
D001439:Bacteroides / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006146:Guanidines; D006801:Humans; D017879:Isothiocyanates; D013861:Thiocyanates; D014461:Ultracentrifugation
D004269:DNA, Bacterial; D006146:Guanidines; D017879:Isothiocyanates; D013861:Thiocyanates; C054435:guanidine isothiocyanate; C054436:guanidine thiocyanate