Real-time visualization of oxyradical burst from single neutrophil by using ultrasensitive video intensifier microscopy.
Dynamics of oxyradical burst from single human neutrophils stimulated by opsonized zymosan was visualized by using an ultrasensitive video intensifier microscopy in the presence of chemiluminescence probe. Luminol-dependent photonic burst activities were clearly corresponding to the distribution of zymosan-treated neutrophils. Heterogeneity of photon-bursting period and the maximum photonic intensity among reactive cells was demonstrated. This may reflect functional heterogeneity of the ability to release oxyradicals among neutrophils.
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
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M
Suematsu
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Oshio
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Miura
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Tsuchiya
D005609:Free Radicals; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008163:Luminescent Measurements; D009504:Neutrophils / Q000378:metabolism* / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism*; D010784:Photomicrography; D014741:Video Recording; D015054:Zymosan
D005609:Free Radicals; D015054:Zymosan; D010100:Oxygen