Effects of gamma irradiation on aflatoxins.
The effects of gamma irradiation on a mixture of aflatoxins B1, B2, G1, G2 were studied. Standard solutions of A and B were irradiated at 5, 10, and 20 kGy in a solution of water/DMSO (9 + 1, v/v) by using a 137Cs source. The control (0 kGy) and irradiated samples were subjected to RP-HPLC analyses with methanol/water (4 + 5, v/v) as the mobile phase. Aflatoxin B1 (AFB1) was the most radio-sensitive of the four compounds. The radiosensitivity of the other aflatoxins, was in increasing order: G2, B2, G1. Only about 5% of AFB1 remained after irradiation of solution A at 5 kGy. When the concentration of solution B was increased two-fold, trace amounts of AFB1 remained after irradiation doses of 10 and 20 kGy. Irradiation was found to be suitable for the destruction of aflatoxins in solution.
Turkish Atomic Energy Authority, Lalahan Nuclear Research Institute of Animal Health, Ankara.
B
B
Mutluer
F U
FU
Erkoç
D000348:Aflatoxins / Q000528:radiation effects*; D002851:Chromatography, High Pressure Liquid; D005720:Gamma Rays; D007202:Indicators and Reagents; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000348:Aflatoxins; D007202:Indicators and Reagents; D012997:Solvents