Transformation of rodent immortalized and embryo cells by oncogenes. I. Mouse (NIH 3T3) and rat (FR 3T3) immortalized fibroblasts.
Immortalized mouse NIH 3T3 cells were transformed by gene transfer of DNA isolated from a human bladder tumor cell line and plasmids containing an activated human Ha-ras oncogene insert. For gene transfer the calcium-phosphate co-precipitation method was used. Transformation was evaluated by morphological focus formation, growth in soft agar and tumor development in nude mice. In addition, immortalized rat FR 3T3 cells were transformed by Ha-ras, too. The co-transfer of ras and myc oncogenes did not enhance focus formation in FR 3T3 cells.
Central Institute for Cancer Research, Robert-Rössle-Institute, Academy of Sciences, GDR Berlin-Buch.
J
J
Denner
I
I
Ehm
B
B
Jandrig
D
D
Friese
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005347:Fibroblasts; D051379:Mice; D009857:Oncogenes; D051381:Rats; D014162:Transfection