Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction.
In this paper we describe methods of preparing orientated f-actin and reconstituting thin filaments that are suitable for X-ray diffraction that allow us to analyse the structure of f-actin to at least 15 A resolution (1 A = 0.1 nm). We described problems that occur during the process of orientation and ways of solving them.
Max Planck Institute for Medical Research, Heidelberg, Federal Republic of Germany.
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D
Popp
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Lednev
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W
Jahn
D000199:Actins; D000818:Animals; D005782:Gels; D008859:Microscopy, Polarization; D009132:Muscles / Q000032:analysis; D011817:Rabbits; D014961:X-Ray Diffraction / Q000379:methods
D000199:Actins; D005782:Gels