Determination of acetylcholine and choline in microdialysates from spinal cord of rat using liquid chromatography with electrochemical detection.
A method for the determination of acetylcholine and choline in microdialysates from rat spinal cord has been developed using liquid chromatography, enzyme-catalysed post-column derivatization and electrochemical detection. The sensitivity required was achieved by a certain degree of miniaturization, careful temperature control and a slight modification of the amperometric detector. Determinations were performed on 5-microliter samples.
Tyrefors
N
N
Department of Analytical Chemistry, Uppsala University, Sweden.
Gillberg
P G
PG
D000109:Acetylcholine / Q000032:analysis*; D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D002794:Choline / Q000032:analysis*; D002853:Chromatography, Liquid; D003956:Dialysis; D004563:Electrochemistry; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000032:analysis*
D007202:Indicators and Reagents; D000110:Acetylcholinesterase; D002794:Choline; D000109:Acetylcholine