Detection of mucus glycoconjugates in human cervical epithelium by lectin-colloidal gold technique in transmission electron microscopy. I. A quantitative study during the menstrual cycle.
We investigated the presence and distribution of five glycosidic residues at the level of the endocervical columnar epithelial cells during the various phases of the menstrual cycle in healthy subjects. We utilized the lectins Wheat Germ Agglutinin (WGA), Peanut Agglutinin (PNA), Soybean Agglutinin (SBA), Concanavalin A (ConA) conjugated with colloidal gold particles as ultrastructural markers in Transmission Electron Microscopy. This technique proved to be particularly suitable for detecting glycosidic residues at the cellular level, where the mucus is produced. All the lectin-binding sites (except ConA binding sites which were not detected) appeared to be produced in significantly different amount during the cycle, supporting the hypothesis that the cyclic-related changes of the cervical mucus viscoelasticity is due to glycoconjugate cyclic variations.
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Istituto di Microscopia Elettronica Clinica, Università di Bologna, Italy.
Maltarello
M C
MC
Costa
S
S
Poggi
M G
MG
Laschi
R
R
D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D000293:Adolescent; D000328:Adult; D002582:Cervix Mucus / Q000032:analysis* / Q000166:cytology; D004187:Disaccharides / Q000032:analysis; D004847:Epithelial Cells; D005260:Female; D006001:Glycoconjugates / Q000032:analysis*; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D037102:Lectins; D008358:Mannose / Q000032:analysis; D008598:Menstruation; D008854:Microscopy, Electron; D012794:Sialic Acids / Q000032:analysis
D004187:Disaccharides; D006001:Glycoconjugates; D037102:Lectins; D012794:Sialic Acids; D006046:Gold; D000116:Acetylgalactosamine; D008358:Mannose; D000117:Acetylglucosamine