Effect of the absence of neonatal testosterone imprinting on the activity of the microsomal enzyme system and on the dexamethasone binding of the thymus in adulthood.
Healthy and neonatally castrated male rats were treated with testosterone twice perinatally, while other groups were treated with testosterone also in adulthood or received testosterone only in adulthood. Castration resulted in a moderate (but in some instances significant) decrease of PSMO (polysubstrate monooxygenase) level measured in adulthood. The decrease could partially be compensated by perinatal testosterone treatment. Further testosterone treatment administered in adulthood did not result in further alteration when compared either with the controls or with the neonatally treated animals. However, since in the controls the second testosterone treatment (following the neonatal one), had a decreasing effect, therefore the testosterone treatment administered in adulthood was responsible for the disappearance of the difference between the castrated animals and the controls treated both perinatally and in adulthood. On the basis of these findings it seems likely that the perinatal presence of testosterone plays a major role in the development of enzymatic imprinting and thus, in securing the capability of the liver to split testosterone in adulthood. Since testosterone influences the glycocorticoid receptors of the thymus (presumably by its overlapping effect), so the amount of free glycocorticoid receptors is always higher in the animals castrated neonatally than in the controls. Conversely, neonatal testosterone treatment somewhat increases the number of receptors detectable in adulthood.
Department of Biology, Semmelweis University Medical School, Budapest, Hungary.
G
G
Csaba
O
O
Dobozy
A
A
Inczefi-Gonda
S
S
Szeberényi
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D003907:Dexamethasone / Q000378:metabolism*; D007173:Imprinting, Psychological / Q000502:physiology*; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D009919:Orchiectomy; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology / Q000502:physiology*; D013950:Thymus Gland / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; C028697:dexamethasone receptor; D013739:Testosterone; D003907:Dexamethasone