Determination of erythromycin in human plasma, using column liquid chromatography with a polymeric packing material, alkaline mobile phase and amperometric detection.
A method based on column liquid chromatography was developed for determination of plasma concentrations of erythromycin. PRP-1, a polymeric type of packing material suitable for chromatography and amperometric detection at high pH, was used. The effect of pH on the column performance and on the electrochemical response was studied. A pH of ca. 10 was found to be optimal. After extraction with tert.-butyl methyl ether, plasma concentrations down to 0.2 mumol/l could be measured, using automated sample injection. Oleandomycin was used as internal standard. The method was used for determination of plasma concentrations in a pharmacokinetic study under steady-state conditions.
Department of Clinical Pharmacology, University Hospital of Lund, Sweden.
L G
LG
Nilsson
B
B
Walldorf
O
O
Paulsen
D002853:Chromatography, Liquid; D004355:Drug Stability; D004563:Electrochemistry; D004917:Erythromycin / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009827:Oleandomycin / Q000097:blood; D011108:Polymers
D011108:Polymers; D004917:Erythromycin; D009827:Oleandomycin