Cytoplasmic transfer between endothelium and lymphocytes: quantitation by flow cytometry.
Interactions between human lymphocytes and vascular endothelial cells in coculture have been examined using a newly developed fluorescence flow cytometric technique. Endothelium and blood lymphocytes are capable of bidirectional transfer of a cytoplasmic fluorescent dye, suggesting a novel path of communication between circulating blood cells and the vessel wall. This method also may be applicable to the analysis of other heterocellular interactions.
Division of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts.
E C
EC
Guinan
B R
BR
Smith
P F
PF
Davies
J S
JS
Pober
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D003593:Cytoplasm / Q000502:physiology*; D004285:Dogs; D004727:Endothelium / Q000502:physiology*; D005434:Flow Cytometry; D008214:Lymphocytes / Q000502:physiology*