Comparison of the effects of 4-methylhistamine on the cell-cycle response between CFU-s and its subpopulation.
When bone marrow cells were treated with an H2-receptor agonist (4-methylhistamine 10(-8) M) before treatment with hydroxyurea (10(-3) M), the suicide rate of spleen colony-forming units (CFU-s) as a whole rose significantly. The rabbit antimouse brain serum (RAMBS)-resistant CFU-s subpopulation was also significantly elevated. Prior treatment of bone marrow cells with cimetidine would prevent this effect of 4-methylhistamine (4-MH). These findings not only confirm that 4-MH can trigger mouse bone marrow CFU-s to enter a DNA synthetic phase of the cell cycle, but also suggest that the RAMBS-resistant CFU-s subpopulation is more sensitive to the effect of 4-MH. Furthermore, this suggests that concentrations of the histamine receptor change during the developmental process of CFU-s.
Research Laboratory of Blood Physiology, Human Medical College, People's Republic of China.
Z C
ZC
Tang
Y H
YH
Xu
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D002927:Cimetidine / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006918:Hydroxyurea / Q000494:pharmacology; D008297:Male; D008761:Methylhistamines / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology
D008761:Methylhistamines; C025566:4-methylhistamine; D002927:Cimetidine; D006918:Hydroxyurea