A new lamin in Xenopus somatic tissues displays strong homology to human lamin A.
The nuclear lamina of vertebrates is composed of several major polypeptides that range in mol. wt from 60 to 80 kd. In mammals, the three major lamin proteins are designated A, B and C. Two major lamins have been described in Xenopus somatic tissues; two other lamins are expressed primarily in germ cells. We have analysed a cDNA clone encoding a Xenopus lamin that is highly homologous to human lamins A and C. The predicted protein has the carboxy-terminal domain characteristic of human lamin A and is thus a lamin A homologue. Surprisingly, the lamin encoded by the cDNA clone is not one of the known Xenopus lamins. The encoded protein is distinct in size from the oocyte lamin LIII and the two somatic lamins LI and LII. Monoclonal antibodies specific for LII, LIII and LIV (the lamin of male germ cells) do not recognize the protein encoded by the cDNA clone; conversely, a polyclonal antibody against the encoded protein does not recognize any of the known Xenopus lamins. This lamin is expressed late in embryonic development, and is present in all adult somatic cells examined, except erythrocytes. Thus frogs and mammals are similar in having three major somatic lamins that fall into distinct structural classes.
Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143.
S L
SL
Wolin
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G
Krohne
M W
MW
Kirschner
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D005260:Female; D006801:Humans; D034904:Lamin Type A; D034882:Lamins; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic; D014981:Xenopus
D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D004247:DNA