Effects of cardiotoxin D from Naja naja siamensis snake venom upon murine splenic lymphocytes.
Cardiotoxin D from Naja naja siamensis is cytotoxic to T-lymphocytes above 150 femtomoles/10(6) cells. Equivalent lysis of macrophages or B-lymphocytes requires at least 1000 times more toxin. Reduction and carboxamidomethylation of cardiotoxin D does not effect T cell lysis. At higher toxin concentrations, 50% T-cell lysis occurs within 10 min. Splenocytes cultured with mitogens are up to five times more susceptible to toxin than unstimulated cells. Cardiotoxin D may directly disrupt the plasma membrane, since lysis is unaltered at 4 degrees C.
Hinman
C L
CL
Department of Medicinal Chemistry and Pharmacognosy, University of Toledo, OH.
Lepisto
E
E
Stevens
R
R
Montgomery
I N
IN
Rauch
H C
HC
Hudson
R A
RA
D000818:Animals; D004179:Cobra Cardiotoxin Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D004546:Elapid Venoms / Q000032:analysis* / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D013154:Spleen / Q000166:cytology
D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms