The use of separator isoelectric focusing in micro-ultrathin polyacrylamide gels in the characterization of some polymorphic proteins of forensic science significance.
The identification of phenotypes of erythrocyte acid phosphatase (EAP), esterase D (EsD), group specific component (Gc), and alpha-1-antitrypsin (PI) by separator isoelectric focusing in micro-ultrathin polyacrylamide gels (interelectrode distance: 45 mm) is described. The protein patterns obtained are compared favorably with the patterns seen by isoelectric focusing in conventional polyacrylamide gel dimensions (interelectrode distance: 110 to 120 mm). The technique described allows greater stability of pH gradients and is a fast and economic method.
Alonso
A
A
Biology Section, National Institute of Toxicology, Madrid, Spain.
Gascó
P
P
D001797:Blood Protein Electrophoresis / Q000379:methods*; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007527:Isoenzymes / Q000097:blood; D008297:Male; D010641:Phenotype
D007527:Isoenzymes