Interaction of the antitumor Au(I) complex [Au(Ph2P(CH2)2PPh2)2]Cl with human blood plasma, red cells, and lipoproteins: 31P and 1H NMR studies.
The bis-chelated tetrahedral gold(I) complex [Au(dppe)2]Cl, where dppe is Ph2P(CH2)2PPh2, is active in several animal tumor models. When added to human blood plasma in vitro it appears to bind to lipoproteins, giving a slightly broadened 31P NMR signal, and 1H NMR resonances which are too broad to detect. Some lipoprotein is denatured. 31P NMR studies suggest that some [Au(dppe)2]+ is transferred from plasma to red cells with a half-life of ca. 2 hr. The complex binds within red cell membranes and the 1H resonances of intracellular glutathione are unaffected. The 31P NMR resonance from [Au(dppe)2]+ in red cell membranes is observable only when the complex is mobilized by addition of sodium dodecyl sulphate, which also mobilizes membrane phospholipids.
Berners-Price
S J
SJ
Department of Chemistry, Birkbeck College, University of London, UK.
Sadler
P J
PJ
D000970:Antineoplastic Agents / Q000097:blood*; D011232:Chemical Precipitation; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006859:Hydrogen; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D050607:Organogold Compounds; D009942:Organometallic Compounds / Q000097:blood*; D009943:Organophosphorus Compounds / Q000097:blood*; D010758:Phosphorus; D011485:Protein Binding
D000970:Antineoplastic Agents; D008074:Lipoproteins; D050607:Organogold Compounds; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; D010758:Phosphorus; C050185:bis(1,2-bis(diphenylphosphino)ethane)gold(I); D006859:Hydrogen