[The dose-effect of benz(a)pyrene in organ cultures of embryonic mouse lungs resistant and predisposed to pulmonary blastomogenesis].
A direct effect of benz(a)pyrene (BP) was studied on organ cultures from embryonic lungs of C57Bl and A mice. The toxic effect of 3, 6, 12 micrograms/ml BP was observed in alveolar epithelium and mesenchymal cells after 7 days of cultivation. After 14-21 days diffuse and focal hyperplasia of the epithelium was discovered in 35.5, 20.0 and 36.1% of treated cultures (explants) from embryonic lungs of C57Bl mice and in 45.5, 56.3 and 53.2% of treated explants from embryonic lungs of A mice. Squamous epithelial metaplasia was observed in 3.0, 2.2 and 4.2% of treated explants from C57Bl mice and in 2.4, 10.4 and 23.4% of treated explants from A mice. Total papillary adenomatous growth of the epithelium was discovered in 10.0, 18.3 and 36.1% of treated explants from embryonic lungs of A mice only. Thus, a direct effect of BP on organ cultures from embryonic lungs of C57B1 and A mice depended on BP doses and the mouse line.
Gor'kova
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TG
Kolesnichenko
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D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000473:pathology; D004198:Disease Susceptibility; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000473:pathology; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D013997:Time Factors
D001564:Benzo(a)pyrene
Doza-éffekt benz(a)pirena v organnykh kul'turakh émbrional'nykh legkikh mysheĭ, ustoĭchivykh i predraspolozhennykh k blastomogenezu legkikh.