A new procedure for the measurement of the outflow facility in conscious rabbits.
A new procedure for measuring the outflow facility in conscious rabbits is described. The Langham pneumatic tonometer is applied horizontally against the eye; the intraocular pressure (IOP) is recorded before, during and immediately following 2 min of a pre-determined increased ocular pressure that is maintained at a fixed value by digital pressure applied through the eyelids. An increased volume of aqueous humor outflow resulting from the IOP increase is evaluated from the initial and final IOP values and the pressure volume relation for eyes of living rabbits. Close agreement in values of the outflow facilities in pairs of eyes of individual rabbits and excellent reproducibility of the procedure were found in repeated measurements made over a 24-hr period. The mean values of the IOP and the total outflow facility in 60 eyes of 30 rabbits were 20.5 +/- 0.2 mmHg and 0.17 +/- 0.01 microliter min-1 mmHg-1 respectively. Thirty minutes after an intravenous injection of acetazolamide, the IOP had decreased in both eyes of individual rabbits. This was associated with a decrease in the outflow facility and with a decrease of more than 50% in the rate of aqueous humor formation. One hour after the unilateral application of epinephrine the IOP had decreased in the treated eyes while the outflow facility remained unchanged.
Ocular Pharmacology and Therapeutics Unit, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
M E
ME
Langham
N
N
Edwards
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects / Q000502:physiology*; D003243:Consciousness; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D007429:Intraocular Pressure / Q000187:drug effects; D008297:Male; D008722:Methods; D011817:Rabbits; D013997:Time Factors; D014065:Tonometry, Ocular
D000086:Acetazolamide; D004837:Epinephrine