Gas chromatographic determination of 1- and 3-methylhistidine in biological fluids.
A gas chromatographic method for the determination of 1- and 3-methylhistidine in biological fluids was developed. The amino acid fraction containing 1- and 3-methylhistidine was isolated using ion-exchange chromatography. The amino acids were derivatized to N-trifluoroacetyl-O-isobutyl esters and analysed by gas chromatography with micropacked or capillary columns and an ionization-resonance detector. The conditions for the acylation of the histidines were studied and analyses of various derivatives such as N-trifluoroacetates, N-pentafluoropropionates and N-heptafluorobutyrates were tested. The detection limit of 1- and 3-methylhistidine was 50 ng per sample.
Scientific Research Institute of Physical Culture, Leningrad, U.S.S.R.
V A
VA
Rogoskin
A I
AI
Krylov
N S
NS
Khlebnikova
D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D006639:Histidine / Q000031:analogs & derivatives*; D006801:Humans; D007202:Indicators and Reagents; D008762:Methylhistidines / Q000032:analysis* / Q000097:blood / Q000652:urine
D007202:Indicators and Reagents; D008762:Methylhistidines; C028120:1-methylhistidine; D006639:Histidine; C028118:3-methylhistidine