Effect of a new non-steroidal anti-inflammatory agent on lysosomal stability in adjuvant induced arthritis.
The effect of new non-steroidal anti-inflammatory agents on lysosomal stability was studied by determining the activity of beta-glucuronidase, a typical lysosomal enzyme, in various sub-cellular fractions and its release from the lysosome-rich fraction. Adjuvant arthritic animals showed a significant increase in the beta-glucuronidase activity in sub-cellular fractions. The increased rate of the release of beta-glucuronidase from lysosome-rich fraction clearly suggested that arthritic syndrome caused decreased stability of the lysosomes. Administration of boswellic acids or salai-guggal to arthritic animals was found to increase the lysosomal stability by inhibiting the rate of release from lysosome-rich fraction and reducing beta-glucuronidase activity in various sub-cellular fractions. Of the two anti-inflammatory agents tested, salai-guggal was found to afford more therapeutic value than boswellic acids.
Department of Biochemistry, Central Leather Research Institute, Madras, India.
G K
GK
Reddy
S C
SC
Dhar
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001168:Arthritis / Q000201:enzymology*; D001169:Arthritis, Experimental / Q000201:enzymology*; D008247:Lysosomes / Q000187:drug effects*; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects; D051381:Rats; D012116:Resins, Plant; D014315:Triterpenes / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012116:Resins, Plant; D014315:Triterpenes; C049844:salai guggal; C054625:boswellic acid