Novobiocin induces accumulation of a single strand of plasmid pGRB-1 in the archaebacterium Halobacterium GRB.
Treatment of Halobacterium GRB cells with the DNA topoisomerase II inhibitor novobiocin induces the accumulation of a circular single-stranded DNA form of the plasmid pGRB-1. This form corresponds to the transcribed strand of pGRB-1. A tiny amount of this form is detectable in untreated cells. The induction of single-stranded pGRB-1 molecules by novobiocin is abolished when cells are pretreated with aphidicolin or anisomycin, which inhibit halobacterial DNA replication and protein synthesis, respectively. These results suggest that the single-stranded form of pGRB-1 is generated in the course of plasmid replication.
Laboratoire de Biologie Moléculaire de la Replication, CNRS-IRSC, Villejuif, France.
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M
Sioud
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G
Baldacci
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P
Forterre
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AM
de Recondo
D001426:Bacterial Proteins / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004270:DNA, Circular / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000187:drug effects* / Q000648:ultrastructure; D006217:Halobacterium / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D009675:Novobiocin / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects*; D014158:Transcription, Genetic / Q000187:drug effects
D001426:Bacterial Proteins; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D009675:Novobiocin