The participation of antibody in spontaneous and antibody-dependent cell-mediated cytotoxicity in the pig.
Treatment of porcine lymphocytes with trypsin reduced their spontaneous cell-mediated cytotoxicity (SCMC) activity against target cells persistently infected with transmissible gastroenteritis virus (PK15-TGE cells), but had no effect on antibody-dependent cell-mediated cytotoxicity (ADCC). SCMC activity was partially restored to trypsin-treated lymphocytes by incubation in RPMI-1640 medium or in medium containing F(ab')2 fragments of rabbit anti-porcine immunoglobulin, but not by brief incubation in autologous serum. F(ab')2 fragments of anti-porcine immunoglobulin did not block the SCMC reaction, but ADCC was greatly reduced by this reagent. Thus SCMC and ADCC mediated by porcine lymphocytes against PK15-TGE target cells clearly involved two distinct mechanisms in terms of antibody participation and sensitivity to trypsin.
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Canada.
A
A
Cepica
J B
JB
Derbyshire
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity; D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008214:Lymphocytes / Q000276:immunology; D012397:Rosette Formation; D013552:Swine; D014357:Trypsin
D000906:Antibodies; D002860:Chromium Radioisotopes; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D014357:Trypsin