Changes in glycosaminoglycan synthesis during the differentiation of HL-60 cells induced by 12-O-tetra-decanoylphorbol-13-acetate.
The changes in glycosaminoglycan (GAG) synthesis during the differentiation of HL-60 cells to macrophage-like cells by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) were studied by using 35S-sulfate or 3H-glucosamine as a tracer. The incorporation of 35S-sulfate into GAGs of HL-60 cells treated with TPA (TPA-treated HL-60 cells) decreased by 30% compared with untreated HL-60 cells (HL-60 cells). The profiles of the Sepharose CL-6B chromatography showed that the molecular weight size of proteoglycans (PGs) in the medium fraction of TPA-treated HL-60 cells was larger than that of HL-60 cells although the PGs in the cell fractions of both HL-60 and TPA-treated HL-60 cells were of the same molecular weight size. On the other hand, the molecular weight sizes of GAGs in both the medium and the cell fractions of TPA-treated HL-60 cells were larger than those of HL-60 cells. In order to examine the synthesis of GAG chains, HL-60 and TPA-treated HL-60 cells were incubated with 35S-sulfate in the presence of 4-methylumbelliferyl-beta-D-xyloside, an exogenous initiator. The incorporation of 35S-sulfate into total GAGs of the TPA-treated HL-60 cells exposed to the beta-D-xyloside increased 3-fold over that of HL-60 cells. HL-60 cells synthesized core protein-initiated GAGs and beta-D-xyloside-initiated GAGs but TPA-treated HL-60 cells synthesized beta-D-xyloside-initiated GAGs only. beta-D-Xyloside-initiated GAGs synthesized by both cells were obtained in the same fraction by Sepharose CL-6B chromatography.(ABSTRACT TRUNCATED AT 250 WORDS)
Hasumi
F
F
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Tanji
N
N
Mori
Y
Y
D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004187:Disaccharides / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004187:Disaccharides; D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D013755:Tetradecanoylphorbol Acetate