[The determination of the coccidiostat nicarbazin in animal tissue and eggs. I. Determination by pulse polarography and high pressure fluid chromatography with electrochemical detection].
The determination of nicarbazin residues in foodstuffs of animal origin by two different methods, polarography, HPLC with electrochemical detection, is described. By applying pulse polarography at the dropping mercury electrode to acetonitrile extracts of animal tissues and eggs, a detection limit of 50 micrograms/kg and mean recoveries of 79% were obtained. The same extracts were used for the HPLC determination with electrochemical detection. This led to a detection limit of 1 microgram/kg. Additional rapid confirmation can be obtained from HPLC using an internal standard (nifursol) and comparing peak area proportions of both substances at varied polarization voltages. The quantitative determination of nicarbazin in chicken eggs by both polarography and HPLC was found to be in good agreement.
Landesveterinäruntersuchungsamt Rheinland-Pfalz, Koblenz, Bundesrepublik Deutschland.
G
G
Knupp
G
G
Bugl-Kreickmann
C
C
Commichau
T
T
Schmidt
H
H
Büning-Pfaue
D000818:Animals; D002224:Carbanilides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D004563:Electrochemistry; D009528:Nicarbazin / Q000032:analysis*; D011048:Polarography
D002224:Carbanilides; D009528:Nicarbazin
Zur Bestimmung des Coccidiostaticums Nicarbazin in tierischem Gewebe und im Ei. I. Nachweis mittels Pulspolarographie und Hochdruckflüssigchromatographie mit elektrochemischer Detektion.