[24-hour study of thawed erythrocytes. Value of a protective solution].
We have previously shown that thawed RBC concentrate can be stored at +4 degrees C during 9 days if resuspended in a synthetic medium: ESOC. We now report the in vitro evolution of thawed RBC stored with or without protective medium during the 24 hours legal time-limit. (Formula: see text) We show that without protection, the ATP and 2,3-DPG levels remain acceptable, but spontaneous or caused hemolysis is high. The level of free Hb is soon over the legal limit. The addition of our protective medium enhances ATP and hemolysis is strongly reduced. We conclude that a protective medium should be added to all thawed RBC concentrates.
Laboratoire de Cryobiologie, Centre de Transfusion Sanguine de Strasbourg.
V
V
Camberlein
S
S
Goll
D
D
Ehrard
O
O
Kane
D001793:Blood Preservation; D003451:Cryoprotective Agents; D004912:Erythrocytes; D005615:Freezing; D006801:Humans; D013997:Time Factors
D003451:Cryoprotective Agents
Etudes des hématies décongelées pendant 24 heures. Intérêt d'une solution protectrice.