The kinetics of cell cloning assays: hemopoietic spleen colony growth.
The less than self-evident effect of alterations in cell kinetics on the growth of hemopoietic colonies in the spleens of irradiated mice has important implications for the interpretation of results obtained with this unique multipotential stem cell assay. The effect on colony growth of variation in the principle cell kinetic parameters has been calculated by simulating the growth of spleen colonies so as to provide a guide to the interpretation of experimental results. This data is used to re-interpret two particular experimental observations on the effect of cytotoxic agents in terms of alterations in the response of stem cells to regulatory factors in place of previous interpretations regarding the differing self-renewal capacities for subpopulations of stem cells and their sensitivity to various drugs.
Unite de Recherches Cinetique Cellulaire, Institut Gustave Roussy, Villejuif, France.
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Blackett
D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation / Q000528:radiation effects; D002999:Clone Cells; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D013154:Spleen / Q000166:cytology* / Q000528:radiation effects