Redistribution of platelet glycoproteins induced by allo- and autoantibodies.
The redistribution of platelet glycoproteins (GP) Ib, IIb and IIIa in response to stimulation with human platelet specific alloantibodies, autoantibodies and a quinidine-dependent antibody was investigated using immunofluorescence and a quantitative radioimmunoassay. The platelet GPs carrying the corresponding epitopes were determined using immunoblot technique or radioimmunoprecipitation. In accordance with previous results obtained with murine monoclonal platelet specific antibodies, redistribution upon stimulation with human platelet reactive antibodies was observed only due to reactions with epitopes on GP IIb, IIIa, respectively, but not on GP Ib. We therefore believe that membrane redistribution of human platelets induced by various antibodies is a function of the GP recognized by those antibodies rather than the source of the platelet reactive antibody.
Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Giessen, FRG.
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Santoso
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Kiefel
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Mueller-Eckhardt
D000918:Antibody Specificity; D001323:Autoantibodies; D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D004705:Endocytosis; D006801:Humans; D007152:Immunologic Capping; D066298:In Vitro Techniques; D007518:Isoantibodies; D010980:Platelet Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism
D001323:Autoantibodies; D007518:Isoantibodies; D010980:Platelet Membrane Glycoproteins