Granulocytic progenitor cells in the adherent layer of human long-term bone marrow cultures.
The importance of the adherent layer in long-term mouse bone marrow cultures as a reservoir of the most primitive stem cells is known. The role of the adherent cell layer in long-term human cultures (LTC) is examined from this point of view. Confluent adherent layers developed after about 3 weeks of culture. At that time and weekly thereafter the cellularity and granulocytic-macrophage progenitor (CFU-GM) content of the adherent fraction were determined after tripsinisation. We have documented that CFU-GM were present in the adherent layer of human cultures for at least 8 weeks. These findings emphasize the importance of assessing the progenitor cell content of the adherent layer in long-term human cultures.
Second Department of Medicine, University Medical School of Szeged, Hungary.
Z
Z
Borbényi
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NG
Tetla
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G
Varga
D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000502:physiology; D013997:Time Factors