Nucleotide sequence comparisons of turnip yellow mosaic virus isolates from Australia and Europe.
The genomic sequences of four isolates of turnip yellow mosaic virus (TYMV-Cd) from Australia, and three TYMV-1 (type) and three TYMV-2 (cauliflower) isolates from Europe were compared by cDNA-RNA hybridization tests, by analysis of the fragments produced from cDNA-RNA hybrids by restriction endonuclease treatment, and by determining the 3' terminal nucleotide sequences of their coat protein mRNAs. All three methods showed only slight differences (ca. 1%) between the mRNA sequences of different TYMV-1 and TYMV-Cd isolates, and did not distinguish between those groups of isolates. By contrast, the nucleotide sequences of TYMV-2 isolates differed from those of the other TYMVs by ca. 5% (sequence analysis) to 11% (restriction fragment analysis). Published biogeographic evidence has indicated that the TYMV-Cd and TYMV-1 populations probably separated more than 12,000 years ago. This implies that these TYMV genomes have changed at a rate of, at most, 1% in 10,000 years.
Research School of Biological Sciences, Australian National University, Canberra, A.C.T.
J
J
Blok
A
A
Mackenzie
P
P
Guy
A
A
Gibbs
D001315:Australia; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D005060:Europe; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics* / Q000302:isolation & purification; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D004247:DNA