The effect of O-acetylsalicylic acid on lipid synthesis by guinea pig gastric mucosa in vitro.
The aim of this work was to investigate the involvement of lipids as possible components of the gastric mucosal barrier by studying the synthesis and secretion of lipids by the epithelial cell lining of gastric mucosa and the effect of salicylate on these processes. O-Acetylsalicylic acid reversibly reduced in vitro incorporation of [U-14C]acetate and of DL-[2-14C]mevalonic acid into lipids by isolated epithelial cells and by intact mucosa of guinea pig stomach, indicating reversible inhibition of lipid synthesis by the tissue in the presence of the drug. Inhibition of incorporation of both precursors into total lipids, into their fatty acid components, and into cholesterol is demonstrated.
Dept. of Surgery, Guy's Hospital Medical School, London, U.K.
M
M
Spohn
I
I
McColl
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D002784:Cholesterol / Q000096:biosynthesis; D005227:Fatty Acids / Q000096:biosynthesis; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D014176:Protein Biosynthesis
D005227:Fatty Acids; D008055:Lipids; D002784:Cholesterol; D001241:Aspirin