Enzymatic activities of Malayan cobra (Naja naja sputatrix) venoms.
The enzymatic activities of four samples of Malayan cobra venom were investigated. There was significant variation in the contents of L-amino acid oxidase, alkaline phosphomonoesterase, acetylcholinesterase, phospholipase A, 5'-nucleotidase and hyaluronidase. The phosphodiesterase content was, however, constant. Storage of the lyophilized venom powder at 25 degrees C for 1 month did not affect the enzymatic activities. The venom enzymatic activities were generally also stable at 4 degrees C in 0.85% saline solution. After incubation at 37 degrees C for 39 days in 0.85% saline solution, the venom still retained considerable amounts of enzymatic activities. SP-Sephadex C-25 ion-exchange chromatography of the venom showed that the phospholipase A, L-amino acid oxidase, 5'-nucleotidase, phosphodiesterase and alkaline phosphomonoesterase exist in multiple forms.
Tan
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Department of Biochemistry, University of Malaya, Kuala Lumpur, Malaysia.
Tan
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D000818:Animals; D002852:Chromatography, Ion Exchange; D004546:Elapid Venoms / Q000032:analysis* / Q000494:pharmacology; D004798:Enzymes; D005612:Freeze Drying
D004546:Elapid Venoms; D004798:Enzymes