Production of a chemotactic factor for polymorphonuclear leukocytes by epithelioid cells from rat renal glomeruli in culture.
A chemotactic factor for polymorphonuclear leukocytes (PMNs) was found in culture medium of epithelioid cells from rat renal glomeruli. The PMN chemoattractant was trypsin sensitive, heat-stable and eluted from a Sephadex G-50 gel filtration column as a single peak with molecular weight of about 10,000. Lipopolysaccharide stimulated, in a dose-dependent manner, the chemoattractant production by epithelioid cells. This lipopolysaccharide-mediated stimulation was suppressed by an addition of cycloheximide to the culture. These results suggest that component cells of glomeruli itself can attract PMNs by producing a chemotactic factor for PMNs.
Faculty of Pharmaceutical Sciences, Toyama Medical, Pharmaceutical University, Japan.
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Watanabe
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Nakagawa
D000818:Animals; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000378:metabolism* / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003470:Culture Media / Q000032:analysis; D003513:Cycloheximide / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains
D002630:Chemotactic Factors; D003470:Culture Media; D008070:Lipopolysaccharides; D003513:Cycloheximide