Hybridoma cultivation in defined serum-free media: growth-supporting substances. IV. Lipids and serum albumin.
The effect of linoleic acid on growth of hybridomas in serum-free medium was studied. The optimum growth-supporting concentration of linoleic acid was 10 micrograms/ml. Omission of linoleic acid resulted in decreased growth. Hybridoma cells cultivated for 3 days without the fatty acid represented 52-65% of the number of cells growing with the fatty acid. Cholesterol (1 microgram/ml) and dipalmitoyl lecithin (3 micrograms/ml) also stimulated hybridoma growth when medium without linoleic acid was used. However, these substances did not enhance the effect of linoleic acid. Fatty acid-free serum albumin had no effect on hybridoma growth at the concentration (1 mg/ml) used for binding of lipids.
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
J
J
Kovár
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology; D003470:Culture Media; D006825:Hybridomas / Q000187:drug effects*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D051379:Mice; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
D003470:Culture Media; D008041:Linoleic Acids; D015060:1,2-Dipalmitoylphosphatidylcholine; D012710:Serum Albumin, Bovine; D002784:Cholesterol; D019787:Linoleic Acid