Acute regulation of insulin-like growth factor-I gene expression by growth hormone during adipose cell differentiation.
Insulin-like growth factor I (IGF-I) is a mitogenic polypeptide that is thought to play, under the control of growth hormone, a role in fetal development as well as post-natally. The direct effect of growth hormone on the regulation of the expression of IGF-I gene was examined in adipose Ob1771 cells. Growth hormone regulates the abundance of multiple species of IGF-I mRNAs of 15, 7.5, 1.5 and 0.8 kb in a differentiation-dependent manner. The regulation of IGF-I gene expression is strikingly rapid (less than 2 h), reversible and takes place primarily at transcriptional level. Thus growth hormone can increase the cellular content of IGF-I mRNA encoding for a protein which could be involved in a paracrine/autocrine action during adipose tissue development.
Centre de Biochimie du CNRS, Faculté des Sciences, Nice, France.
A
A
Doglio
C
C
Dani
G
G
Fredrikson
P
P
Grimaldi
G
G
Ailhaud
D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013002:Somatomedins / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D012333:RNA, Messenger; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I