[Chromosome radiosensitivity in 2 successive mitotic cycles of human lymphocytes].
A culture of human lymphocytes was irradiated with gamma-quanta (0.5 Gy) after incubation during different time intervals, i.e. at different relative frequencies of cells in the first and second mitotic cycle. The frequency of aberrations produced in the G2 stage was analysed. The total yield of aberrations gradually increased with the increase of time lapse between the onset of cultivation and irradiation. If, however, the relative frequencies of the first and second mitoses are taken into account, it could be concluded that radiosensitivity of chromosomes remains constant in each cycle and independent from its duration. Besides, radiosensitivity of chromosomes during the second cycle is one and half times stronger, as compared to the first cycle. In accordance with the data of other authors, 5-bromodeoxyuridine has been found to significantly increase radiosensitivity.
Luchnik
N V
NV
Poriadkova
N A
NA
D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000187:drug effects / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects*; D011836:Radiation Tolerance; D013997:Time Factors
D001973:Bromodeoxyuridine
Radiochuvstvitel'nost' khromosom v dvukh posledovatel'nykh mitoticheskikh tsiklakh limfotsitov cheloveka.