[Testosterone content of the blood plasma in mature animals of various species during modeling of a pathological process in the testes using testicular antibodies].
Testosterone content in the blood plasma before and after administration of testicular antibodies (IgG and IgM) isolated from respective antisera was studied by a radioimmunoassay in 86 mature mice, rats and guinea-pigs. IgG at a dose of 0.4-2.4 mg of protein per 100 g of body mass decreased testosterone content in the blood plasma 1.6-4.2-fold early after administration (on the 3rd-6th day) in animals of all species. The level of testosterone remained below the basal values in the course of 33 days after the administration of immunoglobulins. A single administration of IgG at a dose of 2.4 mg of the protein resulted in a less damaging effect on the animals' testes than the same amount of the protein administered in fractions (at a dose of 0.8 mg three times a day). IgG produced a higher inhibiting effect than IgM administered at the same doses. Immunoglobulins of class G isolated from antisera which are specific to the testicular tissue, can be used for the simulation of a pathological process in the testes for a study of the etiology and pathogenesis of hypogonadism which is quite frequent in clinical practice.
T M
TM
Zelenskaia
V N
VN
Demchenko
I R
IR
Nikonenko
D000818:Animals; D000918:Antibody Specificity; D001327:Autoimmune Diseases / Q000276:immunology*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007006:Hypogonadism / Q000209:etiology; D007074:Immunoglobulin G / Q000008:administration & dosage; D007075:Immunoglobulin M / Q000008:administration & dosage; D008297:Male; D051379:Mice; D009928:Organ Specificity; D051381:Rats; D013733:Testicular Diseases / Q000276:immunology*; D013737:Testis / Q000276:immunology; D013739:Testosterone / Q000097:blood*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013739:Testosterone
Soderzhanie testosterona v plazme krovi polozrelykh zhivotnykh raznykh vidov pri modelirovanii patologicheskogo protsessa v semennikakh s pomoshch'iu testikuliarnykh antitel.