Phase ratio determination in an ion-exchange column having pores partially accessible to proteins.
A method is suggested for determination of the hold-up volume and the phase ratio of a protein on a strong anion-exchange chromatographic column, which is based on mercury porosimetry and size-exclusion calibration with polymer samples.
Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
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I
Mazsaroff
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FE
Regnier
D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*
D011506:Proteins