[Effect of sex-hormone on composition of rat skin surface lipid].
The optimum analytical conditions for studying the composition of skin surface lipid were examined by high-performance liquid chromatography equipped with a photo-diode array detector. Optimum conditions were as follows: ULTRON N-C18 (150 x 4.6 mm) as stationary phase, acetonitrile/tetrahydrofuran/water (55/35/10, V/V) as eluent at the flow rate of 1.0 ml/min, and column temperature of 40 degrees C. The peaks were detected by monitoring the absorbance at 210 nm. Effect of sex-hormone on composition of skin surface lipids was examined. Gonadectomized Sprague-Dawley rats were injected with either testosterone (50 mg/kg, s.c.) or estradiol (5 mg/kg, s.c.) for 12 days. Amount of crude lipid from the skin surface was decreased at 8 days after castration; estradiol dosing to castrated rats also decreased the amount. The other hand, in ovariectomized rats, testosterone injection increased skin surface lipids. It is recognized that sex-hormone dosing after gonadectomy changes the percentage composition of squalene and cholesterol in male rats, but does not charge them in females.
Imaeda
K
K
Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Tokyo, Japan.
Ohsawa
K
K
Watanabe
T
T
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D050356:Lipid Metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D013185:Squalene / Q000378:metabolism; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology*
D013739:Testosterone; D004958:Estradiol; D013185:Squalene; D002784:Cholesterol