High-performance liquid chromatography measurement of antimicrobial concentrations in polymorphonuclear leukocytes.
High-performance liquid chromatography was used to determine the penetration of 19 antimicrobial agents into human polymorphonuclear leukocytes. The ratios of the intracellular concentration to the extracellular concentration of ampicillin, piperacillin, cefazolin, ceftizoxime, cefpimizole, and ceftazidime were all less than 0.6. Lincomycin showed a high intracellular-to-extracellular ratio (3.0), while clindamycin achieved a ratio of 15.5, which was the highest ratio of all of the 19 tested antibiotics. Ratios for rifampin, isoniazid, chloramphenicol, and trimethoprim were 8.2, 1.1, 9.6, and 6.1, respectively. Six quinolone-class antimicrobial agents had ratios from 2.2 to 8.2. Flucytosine showed a penetration ratio of 4.6. Clindamycin uptake was temperature dependent and occurred best with live polymorphonuclear leukocytes; sodium fluoride, adenosine, and puromycin were inhibitory. The results obtained in this study correlate well with the results of other studies involving radioisotopic methods. This indicates that high-performance liquid chromatography is a useful method for determining the intracellular penetration of antimicrobial agents.
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
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Koga
D000241:Adenosine / Q000494:pharmacology; D000328:Adult; D000900:Anti-Bacterial Agents / Q000097:blood*; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009504:Neutrophils / Q000032:analysis*; D011691:Puromycin / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D000900:Anti-Bacterial Agents; D011691:Puromycin; D012969:Sodium Fluoride; D000241:Adenosine