Immunostimulating cell surface substance from Staphylococcus epidermidis strain ATCC-31432 prevents metastatic lung colonization in Balb/c-mice.
The antineoplastic activity of cell surface substance from Staphylococcus epidermidis ATCC-31432 (CSS-subfraction 2) in mice and its influence on the activation of human leucocytes was studied. After in vitro incubation with CSS-subfraction 2, human polymorphonuclear leucocytes were evidently stimulated. In vivo (Balb/c-mice) intraperitoneal application of subfraction 2 of CSS induced a considerable splenomegaly and greatly increased IgM levels indicating a strong immuno-stimulating activity. In order to evaluate antineoplastic effects of CSS-subfraction 2, we used sarcoma L-1 cells (Balb/c-mouse origin) which cause heavy tumor colonization of the lung. After single systemic injection of subfraction 2 of CSS, the number of lung tumor-cell colonies drastically decreased. The combination of this immunomodulating therapy with a temporary anticoagulation resulted in a further reduction of tumor colonies in the lungs of Balb/c-mice.
Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Japan.
Y
Y
Ohshima
H L
HL
Ko
J
J
Beuth
Y
Y
Ichiman
K
K
Yoshida
G
G
Pulverer
D000276:Adjuvants, Immunologic / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000917:Antibody Formation; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008175:Lung Neoplasms / Q000517:prevention & control / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology*; D012513:Sarcoma, Experimental / Q000517:prevention & control / Q000628:therapy; D013212:Staphylococcus epidermidis
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents