[Formation of the transmembrane electrochemical potential on Staphylococcus cells and membrane vesicles].
The generation of transmembrane difference of electrochemical potentials was registered on the intact cells and ultrasonication-obtained membrane vesicles of Staphylococcus aureus with the application of transmembrane electrophoresis of permeant anions, potassium transport in the presence of valinomycin and 8-anilinonaphthalene-1-sulphonate fluorescence. The membrane potential is formed when the chain of electron transfer or H+-ATPase functions or when the pH gradient varies (the nonenzymic pathway). M-chlorinecarbonylcyanidephenylhydrazonium, a protonophore uncoupler potassium cyanide, an inhibitor of the respiratory chain, N',N-dicyclohexylcarbodiimide, an inhibitor of ATPase, cause the membrane potential dissipation. The orientation of the transmembrane electric field is as follows: "minus" inside cells and "plus" inside membrane vesicles.
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Vinnikov
D001692:Biological Transport; D002463:Cell Membrane Permeability; D004563:Electrochemistry; D005456:Fluorescent Dyes; D008564:Membrane Potentials / Q000187:drug effects; D013010:Sonication; D013211:Staphylococcus aureus / Q000378:metabolism*
D005456:Fluorescent Dyes
Issledovanie obrazovaniia transmembrannoĭ raznosti élektrokhimicheskikh potentsialov na kletkakh stafilokokka i membrannykh vezikulakh.