The adrenal gland of stranded whales (Kogia breviceps and Mesoplodon europaeus): morphology, hormonal contents, and biosynthesis of corticosteroids.
Histochemical assays, hormonal quantitation, and steroid biosynthetic studies were carried out with adrenal glands obtained from four stranded whales of two different species (Kogia breviceps and Mesoplodon europaeus), and selected comparisons were made with the results of similar studies of adrenals from terrestrial mammals (man, beef, rat). Histochemical chemical assays of the whale glands for succinic dehydrogenase activity (SDA) showed an intense SDA-positive reaction in the peripheral cortex, and an SDA-negative central medulla, a pattern similar to that found in terrestrial mammals; the whale adrenals, however, demonstrated a markedly pseudolobulated appearance because of a festooned corticomedullary junction. On radioimmunoassay of preformed cortical steroid hormones, corticosterone (B) exceeded cortisol (F) levels by a factor of 3 in the whale adrenals and aldosterone (Aldo) concentrations were 20-100 times lower than in the terrestrial mammals studied. HPLC determinations of preformed medullary catecholamines showed that, contrary to the findings in the terrestrial mammals studied, norepinephrine predominated over epinephrine and the levels of dopamine were much higher in the whale adrenals. In vitro, surviving sections of whale adrenals elaborated B from endogenous substrates, but not F or Aldo. Incubations of subcellular fractions of the whale adrenals with 14C-labeled precursors resulted in the isolation of several steroid intermediates (pregnenolone, progesterone, deoxycorticosterone) as well as the glucocorticoid end-product B, but again without evidence of the formation of either F or Aldo. In keeping with studies in terrestrial mammals, the enzymatic reactions involved in the conversion of [14C]cholesterol to B occurred under aerobic conditions, required the presence of an exogenous NADPH-generating system, and had identical subcellular localization in the whale adrenals. The process of steroid biosynthesis thus appears generally similar in aquatic and terrestrial mammals. It is possible that some of the unusual findings in the whale adrenals studies here may be related to the profound stress of stranding experienced by these marine mammals.
Research Service, Veterans Administration Medical Center, Miami, Florida 33125.
A
A
Carballeira
J W
JW
Brown
L M
LM
Fishman
D
D
Trujillo
D K
DK
Odell
D000305:Adrenal Cortex Hormones / Q000032:analysis* / Q000096:biosynthesis; D000311:Adrenal Glands / Q000032:analysis* / Q000033:anatomy & histology; D000818:Animals; D002395:Catecholamines / Q000032:analysis; D002417:Cattle; D002591:Cetacea / Q000378:metabolism*; D005260:Female; D005938:Glucocorticoids / Q000032:analysis; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008297:Male; D008901:Mineralocorticoids / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D013045:Species Specificity; D013385:Succinate Dehydrogenase / Q000032:analysis; D014907:Whales / Q000033:anatomy & histology / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D002395:Catecholamines; D005938:Glucocorticoids; D008901:Mineralocorticoids; D013385:Succinate Dehydrogenase