The bromoperoxidase from the lichen Xanthoria parietina is a novel vanadium enzyme.
A novel bromoperoxidase was isolated from the lichen Xanthoria parietina. The enzyme contained vanadium, which is essential for enzymic activity. Under denaturating conditions the preparation showed a single protein band with an Mr of 65,000. Thermal-denaturation studies showed that this bromoperoxidase could tolerate high temperatures. The affinity of the enzyme for its substrate bromide is high; the Km for bromide was 29 microM. Excess halides (50 mM) inhibited enzymic activity considerably.
Plat
H
H
Laboratory of Biochemistry, University of Amsterdam, The Netherlands.
Krenn
B E
BE
Wever
R
R
D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008011:Lichens / Q000201:enzymology*; D010544:Peroxidases / Q000302:isolation & purification / Q000378:metabolism*; D011489:Protein Denaturation; D013696:Temperature; D014639:Vanadium / Q000032:analysis*
D014639:Vanadium; D010544:Peroxidases; C026195:bromide peroxidase