Insect lipid transfer particle catalyzes diacylglycerol exchange between high-density and very-high-density lipoproteins.
Facilitated diacylglycerol exchange between Manduca sexta [3H]diacylglycerol-labeled high-density lipophorin and Heliothis zea very-high-density chromolipoprotein was studied. M. sexta lipid transfer particle was employed in assays which measured exchange of [3H]diacylglycerol. Following incubations with lipid transfer particle, donor and acceptor lipoproteins were reisolated by density-gradient ultracentrifugation to determine facilitated exchange. The reaction was limited to diacylglycerol exchange, while donor or acceptor particle apoprotein exchange did not occur. Lipid analysis of donor and acceptor lipoproteins after the lipid-exchange reaction revealed that the labeled diacylglycerol remained unchanged. Lipid transfer particle-catalyzed diacylglycerol exchange was linear up to 0.3 micrograms lipid transfer particle protein in a standard assay and exchange occurred at a rate of 2.5 micrograms diacylglycerol min-1.micrograms-1 lipid transfer particle protein. The assay method was used to show that the hemolymph concentration of lipid transfer particle increased during development.
Ryan
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Department of Biochemistry, University of Arizona, Tucson 85721.
Haunerland
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NH
Bowers
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Law
J H
JH
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000502:physiology*; D002384:Catalysis; D004075:Diglycerides / Q000378:metabolism*; D005989:Glycerides / Q000378:metabolism*; D006458:Hemolymph / Q000032:analysis / Q000502:physiology*; D008075:Lipoproteins, HDL / Q000302:isolation & purification / Q000378:metabolism*; D009036:Moths
D002352:Carrier Proteins; D004075:Diglycerides; D005989:Glycerides; D008075:Lipoproteins, HDL; C030363:lipid transfer protein; C026277:very high density lipoproteins