Submitochondrial localization of kidney 25-hydroxycholecalciferol 1 alpha-hydroxylase in vitamin D repleted weanling guinea pigs.
We have studied the submitochondrial localization of guinea-pig kidney 25-hydroxycholecalciferol 1 alpha-hydroxylase. Treatment of the mitochondrial-enriched fraction with recrystallized digitonin produced mitoplasts bordered by a single membrane and with intact matrix. They contained nearly 90% of the 25-hydroxycholecalciferol 1 alpha-hydroxylase activity and nearly 100% of the cytochrome-c:oxygen oxidoreductase. Amine:oxygen oxidoreductase activity remained mainly in the outer membrane fraction. These data show that 25-hydroxycholecalciferol 1 alpha-hydroxylase has a distribution similar to that of steroid hydroxylases.
Genetics Unit, Shriners Hospital, Montréal, Que., Canada.
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Yan
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Charette
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Delvin
D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000378:metabolism*; D000818:Animals; D006168:Guinea Pigs; D007668:Kidney / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D013250:Steroid Hydroxylases / Q000378:metabolism*; D013367:Submitochondrial Particles / Q000201:enzymology* / Q000648:ultrastructure
D013250:Steroid Hydroxylases; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase