[In vivo determination of alkaline phosphatase activity of the intestine of the rainbow trout (Salmo gairdneri R.)].
This work has concerned the description of a method suitable for the measurement, on living trout, of the intestinal alkaline phosphatase activity. The experiments consist in the perfusion, in the lumen, of an appropriate substrate and in the prevention of its absorption or that of the products of its hydrolysis. This condition has not been satisfied by using paranitrophenyl phosphate which has been replaced by glucose-1-phosphate. The absorption of glucose produced has been inhibited by phloridzin (10(-4) M) in a KCl (150 mM) solution. Under these conditions an hydrolysis of glucose phosphate has been shown at alkaline pH, which remains linear with time up to 60 min. A research of the Km of the enzyme activity has been carried out.
Institut Michel Pacha, Laboratoire maritime de physiologie, La Seyne-sur-Mer.
D
D
Bussière
G
G
Bogé
G
G
Pérès
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D005947:Glucose / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption / Q000187:drug effects; D007422:Intestines / Q000201:enzymology*; D007700:Kinetics; D008871:Microvilli / Q000201:enzymology; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010695:Phlorhizin / Q000494:pharmacology; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D005958:Glucosephosphates; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; C031590:glucose-1-phosphate; D010695:Phlorhizin; D000469:Alkaline Phosphatase; D005947:Glucose
Mesure in vivo de l'activité de la phosphatase alcaline dans l'intestin de la truite arc-en-ciel (Salmo gairdneri R.).