Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q 32.1-2qter.
A DNA probe derived from a mouse intronless pseudogene including coding regions for the myosin fast skeletal muscle alkali light chains, MLC1F/MLC3F (suggested HGM symbol, MYL1), was tested on a panel of 25 independent man-rodent somatic cell hybrids in order to assign the human MLC1F/MLC3F gene to a human chromosome. A 3.7-kb TaqI human fragment was found to correlate with the presence of chromosome 2 in the hybrids, characterized both by cytogenetic analysis and reference enzyme markers. A regional assignment to 2q32.1-qter was possible using hybrids whose human parental strains bore a reciprocal translocation t(X;2) (p22;q32.1). The fact that IDH1 and the MLC1F/MLC3F gene are closely linked on chromosome 1 in the mouse and map to the same region of human chromosome 2 in man indicates, that these chromosomes have a conserved region of homology between them and that the human 3.7-kb TaqI fragment corresponds indeed to a functional gene.
Cohen-Haguenauer
O
O
INSERM U12, Hôpital des Enfants-Malades, Paris, France.
Barton
P J
PJ
Nguyen
V C
VC
Serero
S
S
Gross
M S
MS
Jegou-Foubert
C
C
de Tand
M F
MF
Robert
B
B
Buckingham
M
M
Frézal
J
J
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009132:Muscles / Q000032:analysis*; D015879:Myosin Subfragments; D009218:Myosins / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics*; D011544:Pseudogenes
D005819:Genetic Markers; D015879:Myosin Subfragments; D010446:Peptide Fragments; D004247:DNA; D009218:Myosins