Hydrochloric acid hydrolysis of proteins and determination of tryptophan by reversed-phase high-performance liquid chromatography.
A simple method was developed for determination of tryptophan in proteins. Hydrolysis is achieved under reducing conditions, in 6 N hydrochloric acid containing 0.4% beta-mercaptoethanol, at 110 degrees C for 24 h. The phenylthiocarbamyl derivatives of the amino acids are separated by reversed-phase high-performance liquid chromatography, without any by-product interference. The recovery of tryptophan is complete. However, the method does not allow the determination of tryptophan in carbohydrate-rich biological samples.
Physiologie Métabolique et Nutrition, Université Pierre et Marie Curie, Paris, France.
L T
LT
Ng
A
A
Pascaud
M
M
Pascaud
D000596:Amino Acids; D002851:Chromatography, High Pressure Liquid; D006851:Hydrochloric Acid; D006868:Hydrolysis; D011506:Proteins; D014364:Tryptophan / Q000032:analysis*
D000596:Amino Acids; D011506:Proteins; D014364:Tryptophan; D006851:Hydrochloric Acid