Comparison of two methods for measurement of chemotaxis of neutrophil polymorphonuclear leukocytes in vitro.
Measurement of chemotaxis of neutrophil polymorphonuclear leukocytes (PMNs) is often performed by micropore-filter methods. However, most of these methods seem to involve considerable inter-individual, intra-individual, and day-to-day variations. The aim of this study was to compare two frequently used micropore-filter methods - the Lower Surface Count (LSC) and the Leading Front (LF) methods - in order to find the more reproducible one. Blood samples from the same individual were drawn on two different days. PMN chemotaxis and random migration was tested by a micropore-filter assay. The chemotaxis chambers were incubated for 60, 90, 120, and 150 minutes, respectively. In all, 144 filters were read by both methods. The LSC method showed large variations both among identically treated filters and among readings from the same filter. These variations were as much as 10 times higher than the variations shown by the LF method. Also, the day-to-day variation was higher with the LSC method than with the LF method. Furthermore, data from the LF method were normally distributed, in contrast to data from the LSC method. Thus, the present study suggests the LF method to be superior to the LSC method in terms of reproducibility.
Department of Periodontology, Royal Dental College, Copenhagen, Denmark.
M M
MM
Pedersen
D002364:Caseins; D002630:Chemotactic Factors; D002634:Chemotaxis, Leukocyte; D006801:Humans; D008853:Microscopy / Q000379:methods; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*
D002364:Caseins; D002630:Chemotactic Factors