Evaluation of changes in cytoplasmic pH in thrombin-stimulated human platelets.
Thrombin causes a dose-dependent cytoplasmic alkalinization of normal human platelets. The pH probe 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) permits an easier and more accurate quantitation of the kinetics of this change previously measured with 9-aminoacridine and 6-carboxyfluorescein (6-CF). We report here a modification of the previously published 6-CF technique and confirm the thrombin-induced cytoplasmic pH change in the human platelet using a second fluorescein derivative, BCECF. Maximal thrombin stimulation raises the resting cytoplasmic pH of the human platelet from 7.0 to 7.25.
Davies
T A
TA
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118.
Dunn
J M
JM
Simons
E R
ER
D001792:Blood Platelets / Q000378:metabolism*; D003593:Cytoplasm / Q000032:analysis*; D005452:Fluoresceins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013917:Thrombin / Q000494:pharmacology*
D005452:Fluoresceins; D013917:Thrombin