The effect of SDS and 2-mercaptoethanol on the supramolecular structure of human gastric mucus gel.
The solubility of freshly obtained human gastric mucus gel was investigated using solutions of Tris buffer, urea, SDS, and 2-mercaptoethanol. The best solubility was achieved in SDS solution, whereas 2-mercaptoethanol did not solubilize mucus gel. The degree of mucus depolymerization with the solubilizers was evaluated using light scattering and viscosity measurements. In mucus fractions, neutral carbohydrate, protein, unsaturated fatty acids, and cholesterol were determined. Some aspects of mucus composition and structure are discussed.
Gindzieński
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A
Research Laboratory of Biochemistry, Institute of Chemistry, Medical Academy, Mickiewicza, Poland.
Zwierz
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K
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology*; D009093:Mucus / Q000032:analysis*; D009391:Nephelometry and Turbidimetry; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D012995:Solubility; D014783:Viscosity
D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol