"Matrigenin" activity from bovine bone--II. Effects on the glycosaminoglycans of bovine articular cartilage in culture.
1. Bovine articular cartilage slices were studied in long term culture by periodically pulse-labelling the cultures with radiolabelled precursors of glycosaminoglycans and isolating the glycosaminoglycans from cartilage. 2. Pretreatment of the cartilage slices with bacterial collagenase resulted in stimulation of the incorporation of radioactivity into the glycosaminoglycans. 3. The addition of a fraction from bovine bone, enriched in "matrigenin" activity, to cultures of cartilage pretreated with collagenase resulted in an additional increase in the stimulation of incorporation of radioactivity.
Department of Medicine and Biochemistry, Queen's University, Kingston, Ontario, Canada.
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Brown
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Anastassiades
D000818:Animals; D001842:Bone and Bones / Q000032:analysis; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D009924:Organ Culture Techniques; D014020:Tissue Extracts / Q000494:pharmacology*
D006025:Glycosaminoglycans; D014020:Tissue Extracts; C054630:matrigenin