Purification of human placental acid alpha-mannosidase by an immunological method.
An immunoaffinity column was used for the purification of alpha-mannosidase from human placenta. The enzyme was purified to homogeneity by extraction in the presence of various protease inhibitors, immunoaffinity chromatography, Ultrogel AcA-34 gel filtration and hydroxyapatite chromatography. Two subunits were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Their molecular weights were 65 kDa and 27 kDa. Heterogeneity of the molecular weight of the large subunit was not observed in our preparation. This method is relatively simple and rapid for obtaining the purified enzyme which is structurally not modified during purification procedures.
Division of Inherited Metabolic Disease, National Institute of Neuroscience, Tokyo, Japan.
A
A
Tsuji
Y
Y
Suzuki
D000596:Amino Acids / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007106:Immune Sera; D007700:Kinetics; D008361:Mannosidases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D043323:alpha-Mannosidase
D000596:Amino Acids; D007106:Immune Sera; D008361:Mannosidases; D043323:alpha-Mannosidase