[The role of glutathione in the interphase death of dividing cells].
A study was made of a change in the content of reduced glutathione (GSH) in Ehrlich ascites tumor (EAT) cells after irradiation with doses evoking their interphase death (ID). GSH content was determined in a suspension of EAT cells fixed by hot ethanol. The postirradiation decrease in the GSH content of the suspension was due to its oxidation by hydrogen peroxide resulting from radiochemical reactions after releasing thereof from cells upon fixation. In the absence of an irradiated medium no changes occurred in the GSH content of EAT cells. It is concluded that ID of EAT cells is not associated with the radiation-induced decrease in the content of GSH, an endogenous antioxidant.
Rybina
V V
VV
Korystov
Iu N
IuN
Degtiareva
O V
OV
Dobrovinskaia
O R
OR
Eĭdus
L Kh
LKh
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology / Q000532:radiotherapy; D002455:Cell Division / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005978:Glutathione / Q000032:analysis / Q000502:physiology* / Q000528:radiation effects; D007399:Interphase / Q000528:radiation effects; D051379:Mice; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D005978:Glutathione
Izuchenie roli glutationa v interfaznoĭ gibeli deliashchikhsia kletok.