[The effect on penetration rate of human spermatozoa into the zona pellucida free hamster oocyte system of monoclonal antibodies against human spermatozoa].
The penetration rate of human spermatozoa in zona pellucida-free hamster oocytes was reduced by 4 monoclonal anti-sperm-antibodies (A-24, B-20, III-3, and VII-5) out of 6 tested. The other 2 (VI-1 and VI-16) left the penetration rates virtually unchanged. The extent of penetration rate reduction depended on the concentrations of antibodies A-24, B-20, III-3, and VII-5; if undiluted, they produced a significant reduction of penetration rates, the largest average reduction being almost 50% with undiluted antibody A-24. Decreasing concentrations of the monoclonal anti-sperm-antibodies resulted in significantly smaller reductions of sperm penetration rates. The reason for the reduced penetration rates observed may be a blockage of receptors on the sperm surface by the named monoclonal anti-sperm-antibodies. This can inhibit enzyme reactions of the spermatozoa, or interfere with the interaction between oocyte and spermatozoa membranes. It is noted that only the antibody III-3 caused agglutination of human spermatozoa in the micro-sperm-agglutination and immobilization tests. Therefore, the agglutination or immobilization of spermatozoa may not be held responsible for the reduced penetration rates after addition of monoclonar antibodies, as opposed to the findings with polyclonal antibodies (Steinberg - 1985&).
Abteilung Frauenheilkunde im Zentrum für Operative Medizin I der Christian-Albrechts-Universität.
K
K
Wellnitz
H H
HH
Riedel
D000818:Animals; D000911:Antibodies, Monoclonal; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009865:Oocytes / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000276:immunology / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*
D000911:Antibodies, Monoclonal
Die Beeinflussung der Penetrationsrate humaner Spermatozoen im zona pellucida-freien Hamster-Oozyten-System durch monoklonale Antikörpern gegen humane Spermatozoen.