Developmental stage specificity of the lymphoid V(D)J recombination activity.
We have examined the level of immunoglobulin gene V(D)J recombination activity in a number of cell lines derived from lymphoid or nonlymphoid lineages. The assay we employed uses extrachromosomal DNA as substrate and thereby avoids difficulties associated with the use of chromosomally integrated substrates. The recombination activity decreases during B-lymphoid development. It is highest at the earliest stages of committed B-cell differentiation and then falls progressively, reaching undetectable levels at the mature B-cell stage. The activity is also present in multipotential progenitors of myeloid cells and in pre-T cells but not mature T cells. No activity was found in several nonhematopoietic cell lines. Recombination was seen only among substrate molecules which had replicated in the eukaryotic cells. Several possible interpretations of this result are discussed.
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
M R
MR
Lieber
J E
JE
Hesse
K
K
Mizuuchi
M
M
Gellert
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D004261:DNA Replication; D005803:Genes, Immunoglobulin; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic
D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region