Role of human serum and cellular factors on the growth of peripheral blood CFU-gm.
The effects of fetal calf serum (FCS), autologous serum (AS) and pooled human serum (PS) on granulocyte-macrophage progenitors (CFU-gm) from normal human peripheral blood mononuclear non-adherent cells (MNAC) were studied to determine optimal growth conditions. PS provided fewer variations and better growth conditions than AS and FCS. Moreover, serum inhibitors affecting both autologous and heterologous CFU-gm were detected in a small group (10%) of donors. Since CFU-gm from different donors display a relatively uniform cell-cycle status and membrane phenotype, under optimal growth conditions the number of CFU-gm probably reflects the size of the circulating myeloid progenitor cell compartment.
Istituto di Medicina Interna, Università di Torino, Italy.
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D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D003165:Complement System Proteins / Q000276:immunology; D004261:DNA Replication; D006098:Granulocytes / Q000166:cytology*; D006133:Growth Substances / Q000097:blood*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D008875:Middle Aged; D011817:Rabbits
D006133:Growth Substances; D003165:Complement System Proteins