The study of mutagenic activity of carcinogens and other chemical agents with Salmonella typhimurium assays: testing of 126 compounds.
126 chemical compounds were tested in the Ames assay with bacterial Salmonella typhimurium of 8 various strains (mainly TA 98 & TA100). For metabolic activation liver S9 from arochlortreated rats was used. In the first group of substances tested (51 compounds with confirmed carcinogenicity for animals) 45 agents possessed mutagenicity and 6 gave false-negative response (1.2-dimethylhydrazine, urethane, DDT, chloroform, carbon tetrachloride, 1.4-dioxane). In group 2 (27 compounds which did not possess carcinogenic activity) 22 agents were correctly identified and the rest 5 gave false-positive response (acrolein, styrene-oxide, acridine-orange, 1-naphtylamine, dichlormethane). After summation of data obtained test sensitivity for both groups together made 88.2%, specificity--81.5%, and predictive value--90%. In group 3 (48 compounds, presented by chemical, pharmaceutical and food industries, in respect of which either there are insufficient data on their carcinogenicity, or they were tested for the first time), 8 appeared mutagenic (1-aminoanthraquinone, 2-aminoanthraquinone, 1-amino-4-chloranthraquinone, 2-amino-3-oxyanthraquinone, Rhodulin blau 5B, dinitrochlorbenzene, nitrosodiphenylamine and 2,3,5-trinitronaphthalene).
Khudoley
V V
VV
Laboratory of Chemical Carcinogenic Agents, Ministry of Health, N. N. Petrov Research Institute of Oncology, USSR.
Mizgireuv
I
I
Pliss
G B
GB
D009152:Mutagenicity Tests