Monohydroperoxides of linoleic acid in endoplasmic lipids of rats exposed to tetrachloromethane.
An accurate method for the quantitative determination of hydroperoxy and hydroxy fatty acids in liver microsomes is presented which involves the use of 18O-labeled internal standards. The method is employed for the determination of hydroperoxides in rat liver microsomes after aerobic incubation with Fe2+/ADP and in microsomes from animals exposed to 75 mg tetrachloromethane/kg body weight. The amounts found after artificial microsomal "lipid peroxidation" are almost two orders of magnitude larger than those in microsomes from tetrachloromethane-exposed animals.
Institut fuer Toxikologie, Universitaet Tuebingen, Federal Republic of Germany.
H
H
Frank
M
M
Wiegand
M
M
Strecker
D
D
Thiel
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005296:Ferrous Compounds / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D005227:Fatty Acids; D005296:Ferrous Compounds; D008041:Linoleic Acids; D008054:Lipid Peroxides; D000244:Adenosine Diphosphate; D019787:Linoleic Acid; D002251:Carbon Tetrachloride