Secretion of a growth inhibitory factor by ZR-75-1 human breast cancer cells.
Cultures of the human mammary carcinoma line ZR-75-1 secrete a growth inhibitory factor (GIF) that, when diluted, slows the growth of MDA-MB-231 and MCF-7 cells. Undiluted "conditioned" media prevents cell division from occurring in both human breast cancer lines. ZR-75-1 cells are unaffected by this factor. The amount of GIF in the culture media is related to the confluency of the ZR-75-1 cells. The activity of this GIF is not altered by DNAse or RNAse but is destroyed by heating or trypsin. Growth inhibition is 85-90% reversible if conditioned media is replaced with fresh media.
Department of Radiotherapy, Mount Sinai School of Medicine, New York, N.Y. 10029.
R
R
Blumenthal
W
W
McLaughlin
J
J
Jordan
E
E
Cryan
W
W
Bloomer
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D003470:Culture Media; D005260:Female; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D014407:Tumor Cells, Cultured / Q000378:metabolism
D003470:Culture Media; D006131:Growth Inhibitors