Rapid hydrolysis of proteins and peptides by means of microwave technology and its application to amino acid analysis.
A rapid heating method of hydrolysis by the use of microwave oven has been applied to amino acid analysis of proteins and peptides. This convenient method has been compared with the conventional 6 N HCl hydrolysis at 110 degrees for 24 h. The advantages of this new method are its expedition and the accurate and comparable results as compared to the tedious conventional technique. The method provides a rapid processing of multiple samples within minutes instead of days and inexpensive access to the important data of amino acid compositions of proteins by the commonly used microwave oven. The necessary change in the design of hydrolysis vials and the safety precautions accompanying this novel use of microwave acid-digestion method are also described.
Chen
S T
ST
Institute of Biochemical Sciences, National Taiwan University, Taipei, Republic of China.
Chiou
S H
SH
Chu
Y H
YH
Wang
K T
KT
D000596:Amino Acids / Q000032:analysis*; D006868:Hydrolysis; D008722:Methods; D008872:Microwaves; D010455:Peptides; D011506:Proteins
D000596:Amino Acids; D010455:Peptides; D011506:Proteins