[Modification of nucleic acids in stabilized complementary complexes. II. Effectiveness and direction of alkylation of dodecadeoxyribonucleotide d(pA-A-C-C-T-G-T-T-T-G-G-C) with 4-(N-2-chloroethyl-N-methylamino)benzylidene derivative of the complementary heptanucleotide d(pC-C-A-A-A-C)-A containing a N-(2-hydroxyethyl)phenazinium residue at the 5'-end].
Modification of dodecadeoxyribonucleotide d(pA-A-C-C-T-G-T-T-T-G-G-C) (I) with a heptanucleotide 4-(N-2-chloroethyl-N-methylamino)benzylidene (RCI) derivative d(pC-C-A-A-A-C) ARCI (II) and with similar reagent (III) bearing an additional 5'-terminal N-(2-hydroxyethyl)phenazinium residue (Phn) has been unvestigated. Both reagents (II) and (III) alkylated dodecanucleotide (I) mainly at the 5'-terminal phosphate, Phn residue not affecting specificity of the alkylation. Stabilization of the complementary complex target oligonucleotide-oligonucleotide derivative by the Phn group resulted in substantial increase of efficiency and rate of the intracomplex alkylation of the dodecanucleotide.
Zarytova
V F
VF
Kutiavin
I V
IV
Podyminogin
M A
MA
Sil'nikov
V N
VN
Shishkin
G V
GV
D000478:Alkylation; D001597:Benzylidene Compounds; D055598:Chemical Phenomena; D002621:Chemistry; D009838:Oligodeoxyribonucleotides; D010619:Phenazines
D001597:Benzylidene Compounds; D009838:Oligodeoxyribonucleotides; D010619:Phenazines
Modifikatsiia nukleinovykh kislot v stabilizirovannykh komplementarnykh kompleksakh. II. Effektivnost' i pozitsionnaia napravlennost' alkilirovaniia dodekadezoksiribonukleotida d(pA-A-C-C-T-G-T-T-T-G-G-C) 4-(N-khlorétil-N-metilamino) benzilidenovym proizvodnym komplementarnogo geptanukleotida d(pC-C-A-A-A-C)-A, soderzhashchim na 5'-kontse tsepi ostatok N-(2-oksiétil)fenaziniia.