Isolation of pure sheaths of Litomosoides carinii microfilariae.
A method is described for the isolation of pure, chemically intact sheaths of blood microfilariae of Litomosoides carinii. Microfilariae were isolated according to standard techniques. Exsheathment was performed by freezing-thawing-shaking procedures, repeated 5-10 times, i.e., larvae were frozen in liquid nitrogen, thawed at room temperature, and shaken vigorously for 5 s. Exsheathment rates were about 50%. Sheaths were separated from ensheathed and exsheathed microfilariae and microfilariae fragments by filtration through a polycarbonate filter (2 micron pore size). The achievable yield (about 15% of the sheaths of a batch of microfilariae) was approximately 1 microgram of sheaths per 10(6) microfilariae.
Biochemisches Institut am Klinikum, Giessen, Federal Republic of Germany.
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G
Bardehle
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Klonisch
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Schott
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Stirm
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Zahner
D000818:Animals; D005370:Filarioidea / Q000033:anatomy & histology*; D005615:Freezing; D008842:Microfilariae / Q000033:anatomy & histology; D014462:Ultrafiltration