Liver cell myosin: isolation and properties.
We have purified myosin from isolated rabbit liver cells that had been previously shown to be well separated from blood vessels and connective tissue (Okamoto, Y. et al. (1983) J. Biochem. 94, 645-653). It comprises a 200-kDa heavy chain and light chains of 24-kDa, 22-kDa, and 17-kDa. In the light chain composition and in the mobility in PPi-PAGE, liver cell myosin differs from the myosin in liver blood vessels. The light chains of liver cell myosin were phosphorylated by myosin light-chain kinase from chicken gizzard and the Mg2+-ATPase activity of phosphorylated myosin was activated 10-fold by F-actin.
Ueno
T
T
Department of Biochemistry, School of Medicine, Juntendo University, Tokyo, Japan.
Sekine
T
T
D000818:Animals; D002645:Chickens; D005895:Gizzard, Avian; D008099:Liver / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000201:enzymology; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D009218:Myosins / Q000302:isolation & purification* / Q000378:metabolism; D010766:Phosphorylation; D011817:Rabbits
D046911:Macromolecular Substances; D009219:Myosin-Light-Chain Kinase; D009218:Myosins