Phase ratio determination in an ion-exchange column having pores partially accessible to proteins.
A method is suggested for determination of the hold-up volume and the phase ratio of a protein on a strong anion-exchange chromatographic column, which is based on mercury porosimetry and size-exclusion calibration with polymer samples.
Mazsaroff
I
I
Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
Regnier
F E
FE
D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*
D011506:Proteins