[Covalent binding of phospholipids to protein globule in a system of reverse micelles].
Acylation of protein amino groups by N'-hydroxysuccinimide ester of N-succinyl phosphatidylethanolamine in reverse micelles of diisooctylsulphosuccinate in hexane was studied. The experiment in a model system (glycine solution in reverse micelles) showed rate of acylation of amino groups to be by over an order of magnitude higher than rate of hydrolysis. Water-soluble proteins (alpha-chymotrypsin and IgG), modified by means of this method, can effectively bind liposomes without disturbing the integrity of liposomal membrane.
Luk'ianov
A N
AN
Klibanov
A L
AL
Kabanov
A V
AV
Torchilin
V P
VP
Levashov
A V
AV
D055598:Chemical Phenomena; D002621:Chemistry; D002918:Chymotrypsin / Q000032:analysis; D008081:Liposomes / Q000032:analysis*; D008823:Micelles; D010743:Phospholipids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D008081:Liposomes; D008823:Micelles; D010743:Phospholipids; D011506:Proteins; D002918:Chymotrypsin
Kovalentnoe sviazyvanie fosfolipidov s belkovoĭ globuloĭ v sisteme obrashchennykh mitsell.