[Complementary-addressed modification of a single-stranded DNA fragment with an iron-porphyrin oligonucleotide derivative].
Hemin was attached covalently to the 5'-terminus of the 14-mer d(pTGACCCTCTT.CCC)rA and was shown, in the presence of oxygen and a reducing agent, to be active in the cleavage of the complementary sequence (position 261-274) in a 303 nucleotide-long DNA fragment. The yield of the cleavage products reached approximately 50%, the cleavage locus comprising two bases (G275 and G276).
Ivanova
E M
EM
Mamaev
S V
SV
Fedorova
O S
OS
Frolova
E I
EI
D004277:DNA, Single-Stranded; D006418:Heme / Q000031:analogs & derivatives; D006427:Hemin; D007202:Indicators and Reagents; D009841:Oligonucleotides
D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D009841:Oligonucleotides; D006418:Heme; D006427:Hemin
Komplementarno-adresovannaia modifikatsiia odnotsepochechnogo fragmenta DNK zhelezoporfirinovym proizvodnym oligonukleotida.