[Formation of a pentose phosphate cycle metabolite, erythrose-4-phosphate, from initial compounds of glycolysis by transketolase from the rat liver].
Using ion-exchange chromatography of sucrose phosphates on Dowex-1, it was demonstrated that the highly purified rat liver transketolase (specific activity 1.7 mumol/min.mg protein) is capable of catalyzing the synthesis of erythrose-4-phosphate, a metabolite of the pentose phosphate pathway non-oxidizing step, from the initial participants of glycolysis, i. e., glucose-6-phosphate and fructose-6-phosphate. As can be evidenced from the reaction course, the second product of this synthesis is octulose-8-phosphate. The reaction was assayed by accumulation of erythrose-4-phosphate. The soluble fraction from rat liver catalyzes under identical conditions the synthesis of heptulose-7-phosphate (but not erythrose-4-phosphate), which points to the utilization of the erythrose-4-phosphate formed in the course of the transketolase reaction by transaldolase which is also present in the soluble fraction. The role of the transketolase reaction reversal from the synthesis of pentose phosphate derivatives to glycolytic products is discussed. The transketolase reaction provides for the relationship between glycolysis and the anaerobic step of the pentose phosphate pathway which share common metabolites, i. e. glucose-6-phosphate and fructose-6-phosphate.
Stepanova
N G
NG
Demcheva
M V
MV
D000818:Animals; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D006019:Glycolysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010427:Pentose Phosphate Pathway; D051381:Rats; D013403:Sugar Phosphates / Q000096:biosynthesis*; D014174:Transketolase / Q000378:metabolism*
D013403:Sugar Phosphates; C026959:erythrose 4-phosphate; D014174:Transketolase
Obrazovanie metabolita pentozofosfatnogo puti--éritrozo-4-fosfata--iz nachal'nykh soedineniĭ glikoliza transketolazoĭ iz pecheni krysy.