Effects of cardiotoxin D from Naja naja siamensis snake venom upon murine splenic lymphocytes.
Cardiotoxin D from Naja naja siamensis is cytotoxic to T-lymphocytes above 150 femtomoles/10(6) cells. Equivalent lysis of macrophages or B-lymphocytes requires at least 1000 times more toxin. Reduction and carboxamidomethylation of cardiotoxin D does not effect T cell lysis. At higher toxin concentrations, 50% T-cell lysis occurs within 10 min. Splenocytes cultured with mitogens are up to five times more susceptible to toxin than unstimulated cells. Cardiotoxin D may directly disrupt the plasma membrane, since lysis is unaltered at 4 degrees C.
Department of Medicinal Chemistry and Pharmacognosy, University of Toledo, OH.
C L
CL
Hinman
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E
Lepisto
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R
Stevens
I N
IN
Montgomery
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Rauch
R A
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Hudson
D000818:Animals; D004179:Cobra Cardiotoxin Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D004546:Elapid Venoms / Q000032:analysis* / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D013154:Spleen / Q000166:cytology
D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms