Sperm wash in three culture media: maximization of motile sperm recovery during swim-up incubation.
Three different culture media commonly used during in vitro gamete manipulations were studied for their efficacy in sperm wash procedure. Highest numbers of motile sperm were recovered at 6 hours following incubation in WT-6 and Ham's F-10 media. However, WT-6 yielded higher motile sperm numbers than Ham's F-10. Swim-up sperm number reached a peak at 3 hours following incubation in BWW. A period of 2 to 6 hours of incubation of sperm pellets overlayed with sperm wash media resulted in highly enriched motile sperm fractions free of dead spermatozoa and seminal debri.
Department of Obstetrics and Gynecology, School of Medicine, Texas Tech University Health Sciences Center, Lubbock.
R
R
Vijayakumar
B
B
Ndubisi
F
F
De Leon
W
W
Heine
D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*
D003470:Culture Media