[Characteristics of DNA replication in the long-term culture of human cells at the stationary phase].
Peculiarities of DNA replication in cultured human diploid fibroblasts in logarithmic and stationary phases were studied using DNA autoradiography. The rate of DNA replication fall from 30-36 mu/hour at active proliferative phase to 18-20 mu/hour at late stationary phase. This phenomenon is characteristic of stationary cultures after stimulation to proliferate by changing medium as well as by culturing without stimulation. Possible mechanisms of DNA replication rate alteration in senescent human cells are discussed.
Iurov
Iu B
IuB
Borsanova
S G
SG
D001345:Autoradiography; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D006801:Humans; D013997:Time Factors
D004247:DNA
Osobennosti replikatsii DNK pri dlitel'nom kul'tivirovanii kletok cheloveka v statsionarnoĭ faze.