Design and construction of a versatile system for the expression of foreign genes in plants.
We have built a series of vectors to allow the constitutive or light-regulated expression of foreign genes in plants. These vectors carry expression cassettes consisting of either the cauliflower mosaic virus 35S promoter or the pea rbcS-E9 promoter, a multiple cloning site derived from M13um20, and the rbcS-E9 polyadenylation site. These cassettes have been incorporated into pBR322-based or RK2-based replicons to facilitate direct DNA uptake or Agrobacterium tumefaciens-mediated gene transfer. Their application for the expression of a bacterial gene is described.
Department of Plant Pathology, University of Kentucky, Lexington 40546-0091.
C L
CL
Schardl
A D
AD
Byrd
G
G
Benzion
M A
MA
Altschuler
D F
DF
Hildebrand
A G
AG
Hunt
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D010944:Plants / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012093:Replicon; D012231:Rhizobium / Q000235:genetics; D014162:Transfection
D004247:DNA