Further studies of oligosaccharide recognition by the soluble 13 kDa lectin of bovine heart muscle. Ability to accommodate the blood-group-H and -B-related sequences.
Oligosaccharide recognition by the 13 kDa soluble lectin from bovine heart muscle has been investigated by inhibition of binding of the 125I-labelled lectin to trypsin-treated rabbit erythrocytes. The results indicate that the Type 1 (Gal beta 1-3GlcNAc) and the Type 2 (Gal beta 1-4GlcNAc) backbone structures are the basic recognition units, and that the blood-group-H structure, the blood-group-B structure, the 'B-like' structure [afucosyl-(blood group B)] and the alpha 2-3 sialylated analogues of the backbone structures can also be accommodated and hence are candidate receptor structures for the lectin. A comparison of available inhibition data on six other soluble beta-galactoside-binding lectins (three from human lung and three from rat lung) has shown some common features among these and the bovine lectin, e.g. in general a stronger reaction with N-acetyl-lactosamine than with lactose, and a lack of reaction with 3-fucosyl-lactose and 6-sialyl-lactose. However, there are distinctive features among the lectins, e.g. differences in relative reactions with the blood-group-A structure, and no two of the lectins appear to be identical in their fine specificities.
Applied Immunochemistry Research Group, MRC Clinical Research Centre, Harrow, Middx. U.K.
W M
WM
Abbott
E F
EF
Hounsell
T
T
Feizi
D000017:ABO Blood-Group System; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D037161:Galectins; D006388:Hemagglutinins / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008968:Molecular Conformation; D009206:Myocardium / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000494:pharmacology
D000017:ABO Blood-Group System; D002352:Carrier Proteins; D037161:Galectins; D006388:Hemagglutinins; D037102:Lectins; D009844:Oligosaccharides; D014357:Trypsin