Dietary control of diacylphosphatidylethanolamine species in brain.
The content and composition of the brain diacylphosphatidylethanolamine species was examined in response to dietary fat intake. Synaptic plasma membrane and microsomal membrane subcellular fractions contain phosphatidylethanolamine species profiles that respond differently to modulation by diet fat. The microsomal content of individual phosphatidylethanolamine species was most responsive to diet treatment and to addition of cholesterol to the diet. Feeding fish oil or linseed oil diets resulted in an increased membrane content of phosphatidylethanolamine species containing six double bonds for both microsomal and synaptic plasma membranes, compared with soya-bean oil- or sunflower oil-fed animals. The 22:5(n - 6) content present in phosphatidylethanolamine species of linseed oil and fish oil-fed animals was also reduced. For microsomal membranes, increase in dietary 18:3(n - 3) resulted in an increased content of phosphatidylethanolamine species containing one double bond. Addition of cholesterol to linseed oil or fish oil diets decreased the microsomal membrane content of phosphatidylethanolamine species containing six double bonds and increased the membrane content of species containing one double bond. For synaptic plasma membrane, addition of cholesterol to linseed oil and fish oil diets increased membrane content of species containing six double bonds. Fish oil-fed animals exhibited a decreased content of species containing a single double bond. The implications of the diet-induced changes in phospholipid species content and composition are discussed.
Department of Foods and Nutrition, University of Alberta, Edmonton, Canada.
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Hargreaves
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Clandinin
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage*; D005227:Fatty Acids / Q000008:administration & dosage; D007425:Intracellular Membranes; D008297:Male; D008861:Microsomes / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013570:Synaptic Membranes / Q000378:metabolism; D014886:Weaning
D002791:Cholesterol, Dietary; D004041:Dietary Fats; D005227:Fatty Acids; D010714:Phosphatidylethanolamines