Synergism of ethanol and clofibrate in the lauric acid hydroxylation.
The effects of ethanol and clofibrate on lauric acid hydroxylation in isolated rat hepatocytes cultured for 72 h were studied. During culture the lauric acid hydroxylation activity decreases. When the hepatocytes were cultured for 72 h with the addition of 10(-5) M, 10(-4) M and 10(-3) M clofibrate, the lauric acid hydroxylation increased by 42%, 428% and 900%, respectively. The stimulation by ethanol was also significant. The addition of both ethanol and clofibrate to the culture media resulted in a potentiation of this induction.
Department of Physiology, Faculty of Biology, University of Santiago de Compostela, Spain.
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JA
Pazo
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Parafita
D000818:Animals; D002994:Clofibrate / Q000494:pharmacology*; D004357:Drug Synergism; D000431:Ethanol / Q000494:pharmacology*; D006900:Hydroxylation; D007850:Lauric Acids / Q000378:metabolism*; D008099:Liver / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007850:Lauric Acids; C030358:lauric acid; D000431:Ethanol; D002994:Clofibrate