[Purification and properties of a growth factor from human bone matrix].
A growth factor was isolated from demineralized human bone-matrix and purified approximately 2,300-fold with 5.6% yield by the procedures of acetone treatment, Blue-A matrix gel chromatography, Sephadex G-75 gel filtration and Mono-Q fast protein liquid chromatography. The final preparation was homogeneous and a single polypeptide of 18,000 daltons as judged by SDS-polyacrylamide gel electrophoresis. The purified growth factor stimulated DNA synthesis in embryonic chick osteoblast in a dose-dependent manner and was saturated at the concentration of 4 ng/ml. By comparing various properties of the final preparation with those of other growth factors derived from bone, it appears to be a novel growth factor, and may be one of the important local regulators of bone remodeling.
Department of Orthopedic Surgery, School of Medicine, Hokkaido University, Japan.
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Tsutsumi
D000818:Animals; D001857:Bone Matrix / Q000032:analysis*; D002642:Chick Embryo; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D006801:Humans; D007335:Insulin-Like Growth Factor II; D010006:Osteoblasts / Q000187:drug effects; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology
C035170:IGF2 protein, human; D011506:Proteins; D007335:Insulin-Like Growth Factor II; D004247:DNA