"Matrigenin" activity from bovine bone--III. Effects on glycosaminoglycans and proteoglycans of human synovial cells in culture.
1. Human synovial fibroblastic cells were cultured in the presence and absence of an extract from bovine bone containing "matrigenin" activity. The rate of incorporation of radioactivity into the glycosaminoglycans of the medium of "matrigenin"-treated cultures increased after 24 hr of incubation, compared to "controls". 2. Higher serum concentrations had a greater effect on the incorporation of radioactivity into hyaluronic acid synthesized by "matrigenin"-treated cultures, than by "controls". 3. Incorporation of radioactive precursors into the proteoglycans isolated from the medium was greater in the "matrigenin"-treated cultures than in "controls". The synthesis of a large mol. wt proteoglycan was specifically stimulated.
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
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Stephens
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Craigie
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Anastassiades
D000818:Animals; D001842:Bone and Bones / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D011509:Proteoglycans / Q000096:biosynthesis*; D013583:Synovial Membrane / Q000187:drug effects* / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology*
D006025:Glycosaminoglycans; D011509:Proteoglycans; D014020:Tissue Extracts; C054630:matrigenin; D006820:Hyaluronic Acid