A microelectronic conductimetric biosensor.
The fabrication and operation of a microelectronic conductimetric biosensor is described. The device monitors the change in solution conductance occasioned by the catalytic action of enzymes immobilised over a planar conductance cell comprising serpentined and interdigitated metal conductor tracks. The output of the instrument was linear over a 3 min period on addition of urea to a sample cell overlaid with immobilised urease. The responses to any given urea concentration were reproducible to within approximately +/- 1%. The device responds to urea present in serum samples.
Biotechnology Centre, University of Cambridge, Great Britain.
L D
LD
Watson
P
P
Maynard
D C
DC
Cullen
R S
RS
Sethi
J
J
Brettle
C R
CR
Lowe
D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008832:Microchemistry; D014508:Urea / Q000032:analysis*; D014510:Urease / Q000378:metabolism*
D004800:Enzymes, Immobilized; D014508:Urea; D014510:Urease