Effects of dietary corn oil and salmon oil on lipids and prostaglandin E2 in rat gastric mucosa.
Three groups of male rats were fed either a corn oil-enriched diet (17%, w/w), a salmon oil-enriched diet (12.5%) supplemented with corn oil (4.5%) or a low-fat diet (4.4%) for eight wk to investigate the possible relationships between dietary fatty acids and lipid composition, and prostaglandin E2 level and phospholipase A2 activity in the rat gastric mucosa. High-fat diets induced no important variation in total protein, phospholipid and cholesterol contents of gastric mucosa. Compared with a low-fat diet, corn oil produced a higher n-6/n-3 ratio in mucosal lipids, whereas this ratio was markedly lowered by a fish oil diet. In comparison with the low-fat diet, the production of prostaglandin E2(PGE2) in gastric mucosa of rats fed salmon oil was significantly decreased by a factor of 2.8. In the corn oil group, PGE2 production tended to decrease, but not significantly. In comparison with the low-fat diet, both specific and total gastric mucosal phospholipase A2 activities were increased (+ 18 and 23%, respectively) in the salmon oil group; they were unchanged in the corn oil group. It is suggested that the decrease of gastric PGE2 in rats fed fish oil is not provoked by a decrease in phospholipase A2 activity but may be the result of the substitution of arachidonic acid by n-3 PUFA or activation of PGE2 catabolism.
INSERM Unité 130, Marseille, France.
R
R
Grataroli
J
J
Léonardi
M
M
Charbonnier
R
R
Lafont
H
H
Lafont
G
G
Nalbone
D000818:Animals; D003314:Corn Oil / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000032:analysis*; D008297:Male; D010741:Phospholipases A / Q000032:analysis; D054467:Phospholipases A2; D010938:Plant Oils / Q000494:pharmacology*; D011458:Prostaglandins E / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012474:Salmon
D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D010938:Plant Oils; D011458:Prostaglandins E; D011506:Proteins; D003314:Corn Oil; D010741:Phospholipases A; D054467:Phospholipases A2