Attempts to increase intratumoral blood flow in the rat solid Walker 256 tumor by the use of the perfluorocarbon emulsion fluosol-DA.
We have examined the effect of the perfluorocarbon emulsion, Fluosol-DA 20% (FDA), on blood flow in rats bearing an advanced solid Walker 256 tumor implanted s.c. Blood-FDA exchange in unanesthetized rats maintained under 100% oxygen was accomplished by simultaneous arterial withdrawal and i.v. infusion until the hematocrit was less than 4%. Control rats were maintained under 100% oxygen but did not undergo any exchange. Regional blood flow studies in tumors of control and FDA-exchanged rats were performed using [14C]iodoantipyrine and quantitative autoradiography. FDA-blood exchange did not increase flow to the whole tumor. Similarly, the pattern of regional flow within the tumor, which was determined in histologically distinct areas--including dense and normocellular, necrotic and peripheral zones invading into muscle and connective tissue--was not substantially altered. Flow to cerebral tissue was increased two-fold, although flow to normal tissues including temporalis muscle, skin and diaphragm was not altered. These results show that FDA-blood exchange does not enhance vascular flow in solid Walker 256 tumor implanted s.c. in the rat.
Klubes
P
P
Department of Pharmacology, George Washington University Medical Center, Washington, DC 20037.
Hiraga
S
S
Cysyk
R L
RL
Owens
E S
ES
Blasberg
R G
RG
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D002279:Carcinoma 256, Walker / Q000098:blood supply*; D004338:Drug Combinations / Q000494:pharmacology; D005466:Fluorocarbons / Q000494:pharmacology*; D006895:Hydroxyethyl Starch Derivatives; D051381:Rats; D012039:Regional Blood Flow / Q000187:drug effects
D001802:Blood Substitutes; D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination