The reaction of Campylobacter species on the chemiluminescence, chemotaxis and hemagglutination.
The influence of Campylobacter species (sp.) on the luminol-dependent chemiluminescence (CL) during phagocytosis, the chemotaxis and the agglutination of different erythrocyte species was investigated. CL was measured directly with undiluted whole blood samples and isolated polymorphonuclear neutrophil granulocytes (PMNG). The chemotactic response of PMNG to various Campylobacter sp. was performed using an agarose technique. All the Campylobacter sp. investigated showed a different CL response pattern. When opsonized Campylobacter strains were used, the CL response of PMNG was greater than in the presence of nonopsonized strains. No correlation was found between CL response and bacterial killing. For the investigation of the chemotaxis the filtered supernatants of Campylobacter cultures were used as cytotoxins and compared with the chemotactic peptide, formyl-methionyl-leucine-phenylalanine (FMLP). All the supernatants of cultures of Campylobacter sp. were chemotactic for PMNG. A strong mannose-resistant hemagglutination (MRHA) with glycine-hydrochloride extract was observed with C. fetus ssp. fetus and a weak reaction, with C. coli. There was no correlation between chemiluminescence, chemotaxis and hemagglutination. The weak stimulation of PMNG by C. fetus ssp. fetus and most of the C. jejuni/coli isolates suggest that this behavior could be a cause of pathogenicity.
Department of Medical Microbiology, University of Kiel, FRG.
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Ullmann
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Krausse
D002167:Campylobacter / Q000276:immunology*; D002634:Chemotaxis, Leukocyte; D006384:Hemagglutination; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins; D010587:Phagocytosis
D009895:Opsonin Proteins