Calmodulin antagonists increase the amount of mRNA for the low-density-lipoprotein receptor in skin fibroblasts.
The effects of calmodulin antagonists on the amount of LDL receptor (LDL-R) mRNA in cultured human fibroblasts was examined by hybridization with a fragment of LDL-R cDNA. In a 'Northern' blot the fragment hybridized to a 5.3-kilobase RNA, as expected for LDL-R mRNA. The concentration of this RNA was increased in preparations from cells that were treated with trifluoperazine or W-7 [N-(6-aminohexyl)-5-chloronaphthalene-1-sulphonamide]. The selectivity of the increase was established by using a probe for beta-actin mRNA. In dot-blot hybridization it was observed that the calmodulin antagonists cause 2-4-fold relative increase in the amount of LDL-R mRNA.
Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Federal Republic of Germany.
H
H
Eckardt
I
I
Filipovic
A
A
Hasilik
E
E
Buddecke
D000199:Actins / Q000378:metabolism; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D011973:Receptors, LDL / Q000187:drug effects / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology
D000199:Actins; D002147:Calmodulin; D012333:RNA, Messenger; D011973:Receptors, LDL; D013449:Sulfonamides; D014268:Trifluoperazine; C017967:W 7