Antioxidant capacity of desferrioxamine and ferrioxamine in the chemically-initiated lipid peroxidation of rat erythrocyte ghost membranes.
2,2'-Azo-bis-(2-amidinopropane) induces the thermal lipid peroxidation of red blood cells membranes by a mechanism that is not iron dependent. The peroxidation rate, as assessed by oxygen uptake or visible chemiluminescence measurements, can be diminished by micromolar concentrations of desferrioxamine (DF), with a median inhibitory concentration (the concentration of DF that reduces the lipid peroxidation rate to 50% of that observed without scavengers addition) of 10 microM. In these conditions, the DF/Fe3+ (1:2) complex is nearly five times less efficient than DF. The present data show that DF is able to trap the initiator radicals and/or the free radicals involved in the lipid peroxidative chain at micromolar concentrations, range in which the agent cannot be used as a general test for iron involvement.
Departamento de Ciencias Biológicas, Facultad de Medicina, Universidad de Chile, Santiago.
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Videla
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Cáceres
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Lissi
D000578:Amidines / Q000494:pharmacology; D000818:Animals; D003676:Deferoxamine / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005290:Ferric Compounds / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D007700:Kinetics; D008054:Lipid Peroxides / Q000097:blood*; D008163:Luminescent Measurements; D008297:Male; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains
D000578:Amidines; D005290:Ferric Compounds; D007502:Iron Chelating Agents; D008054:Lipid Peroxides; C002577:ferrioxamine B; C046728:2,2'-azobis(2-amidinopropane); D003676:Deferoxamine