Regional differences in cytoarchitecture of the weaver cerebellum suggest a new model for weaver gene action.
This paper examines the structure and cytoarchitecture of the cerebellum of the weaver mutant mouse with particular emphasis on regional differences along the mediolateral and anterior-posterior axes. We have uncovered several, previously undescribed features of the weaver cerebellar phenotype. Perhaps the most dramatic example of our findings is the severe disruption of the folial structure of the hemispheres of the weaver cerebellum. A dorsal overgrowth of tissue occurs in the hemispheres that forms a finger-like projection superficial to an atrophic but structurally more normal cerebellar mass underneath. While this folial abnormality is most evident in the homozygote (wv/wv) the antecedents of its appearance are already apparent in the heterozygote (+/wv). At the level of the cytoarchitectonics of the mutant brain, we find substantial variation in the positioning, numbers and density of both Purkinje and granule cells. As a whole, Purkinje plus Golgi II cell numbers are down by over 40%, but this reduction occurs almost exclusively in the medial half of the cerebellum. The hemispheric region contains a nearly normal number of cells per sagittal section (although their positions are predominantly incorrect). The granule cells also show numerical variation; they are nearly absent at the midline, but a substantial number of them survive in the lateral cerebellar cortex. In the paraflocculus, for example, granule cells can be observed in a modest internal granule cell layer as late as 38 postnatal days. These results are discussed in terms of a model of wv gene action in which we propose that the effect of the mutation is a general disruption of cellular distribution in the cerebellar cortex, affecting both Purkinje and granule cells and beginning prenatally.
Herrup
K
K
Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510.
Trenkner
E
E
D000367:Age Factors; D000818:Animals; D002452:Cell Count; D002525:Cerebellar Cortex / Q000166:cytology / Q000254:growth & development / Q000473:pathology; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants / Q000033:anatomy & histology* / Q000254:growth & development; D009474:Neurons / Q000145:classification / Q000473:pathology; D010641:Phenotype