Separation of sialyl-oligosaccharides by medium pressure anion-exchange chromatography on Mono Q.
On columns prepacked with the recently introduced anion-exchange material, Mono Q (Pharmacia), sialyl-oligosaccharides could be fractionated excellently according to the sialic acid content. With u.v. absorption at 214 nm as the detection method, analytical runs of carbohydrate material on the microgram scale, were possible. The value of the method for preparative purposes was demonstrated for sialic acid-containing carbohydrates obtained from human serotransferrin by hydrazinolysis.
Department of Bio-Organic Chemistry, Transitorium III, Utrecht University, The Netherlands.
J
J
van Pelt
J B
JB
Damm
J P
JP
Kamerling
J F
JF
Vliegenthart
D000837:Anion Exchange Resins; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification* / Q000652:urine; D012117:Resins, Synthetic; D014168:Transferrin
D000837:Anion Exchange Resins; D007202:Indicators and Reagents; D009844:Oligosaccharides; D012117:Resins, Synthetic; D014168:Transferrin; C016735:sialooligosaccharides; C042855:Mono Q