DNA repair by articular chondrocytes. IV. Measurement of Micrococcus luteus endonuclease-sensitive sites by alkaline elution in rabbit articular chondrocytes.
The ability of resting and dividing rabbit articular chondrocytes to repair low doses of ultraviolet (UV) damage was measured through removal of UV endonuclease-sensitive sites (ESS, pyrimidines dimers) as measured by alkaline elution. The repair of damage was significantly (P less than 0.001) greater in dividing than non-dividing cells. An age-related decrease in repair capability was found in resting chondrocytes, but not in their dividing counterpart. These results support earlier findings of unscheduled DNA synthesis by the same cells. (Mech. Ageing Dev., 32: 39-55, 1985).
Department of Pathology, State University of New York, Stony Brook 11794.
J M
JM
Lipman
R B
RB
Setlow
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000528:radiation effects; D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair; D004706:Endodeoxyribonucleases; D006863:Hydrogen-Ion Concentration; D008837:Micrococcus / Q000201:enzymology; D009097:Multienzyme Complexes; D009699:N-Glycosyl Hydrolases; D011817:Rabbits; D014466:Ultraviolet Rays
D009097:Multienzyme Complexes; C032219:UV endonuclease; D004706:Endodeoxyribonucleases; D009699:N-Glycosyl Hydrolases