Funnel procedure to separate human sperm with good motility.
Penetration of human sperm into a underlaying Percoll layer is very effective in isolating forward motile sperm. A plastic funnel, the bottom of which having been plugged with a 1.0-ml disposable syringe, was used as a separation vessel. Ejaculated semen (2.0 ml) was loaded on 2.0 ml of 80% Percoll; after allowing to stand 60 min, forward motile sperm penetrating into the lower part of Percoll layer (1.0 ml) were collected into the syringe. Sperm motility was enhanced to 91.5% +/- 3.3% regardless of the original motility (48.4% +/- 14.6%) with a mean recovery of 34.1% +/- 6.8% (n = 22). Measurement of residual lactate dehydrogenase activity suggested that seminal plasma was excluded almost completely from the purified sperm fraction.
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
S
S
Kaneko
H
H
Sato
T
T
Kobayashi
R
R
Iizuka
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D008297:Male; D012661:Semen / Q000032:analysis; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*