Elastolysis of normal and partially cross-linked elastin.
Rate of elastolysis by pancreatic and leukocyte elastases of normal and copper deficient porcine aortic elastins were measured using a conductimetric method. Kinetics obey to Michaelis-Menten model for both substrates and enzymes. KM and Vmax values derived from Lineweaver-Burk plots indicate that, if a near uniformity exists in KM, differences were observed in catalytic rates, kcat increasing approximately 40% for copper deficient elastin elastolysis by leukocyte elastase. This higher susceptibility to proteolysis may have implications for understanding turnover of elastin in tissues.
UA CNRS 244, I.C.B.M.C., Université Lyon I, Villeurbanne, France.
K
K
Bostancioglu
R P
RP
Mecham
J M
JM
Wallach
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D003300:Copper / Q000172:deficiency; D004549:Elastin / Q000378:metabolism*; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism*; D013552:Swine
D003300:Copper; D004549:Elastin; D010196:Pancreatic Elastase