High-performance liquid chromatographic quantitation of triacylglycerols containing fenoprofen from biological samples.
A normal-phase high-performance liquid chromatographic (HPLC) method has been developed for the quantitation of radiolabelled triacylglycerols containing fenoprofen, synthesized from [3H]glycerol by isolated hepatocytes and adipocytes. The assay consists of extracting the lipids into diethyl ether, separating triacylglycerols from polar endogenous lipids using silica Sep-Pak cartridges and quantitating endogenous triacylglycerols and triacylglycerols containing fenoprofen by HPLC resolution and scintillation counting. HPLC separation is achieved in less than 10 min. Using [14C]tripalmitin as internal standard the assay has a linear relationship between added triacylglycerol and measured endogenous triacylglycerols and triacylglycerols containing fenoprofen with regression coefficients of 0.997 and 0.998, respectively.
Sallustio
B C
BC
Department of Clinical Pharmacology, Flinders Medical Centre, Bedford Park, Australia.
Meffin
P J
PJ
Thompson
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005279:Fenoprofen / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000032:analysis / Q000166:cytology; D008297:Male; D010666:Phenylpropionates / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000032:analysis*
D008055:Lipids; D010666:Phenylpropionates; D014280:Triglycerides; D005279:Fenoprofen