Studies on the properties of malic enzyme and malate dehydrogenase from Taenia crassiceps (Zeder, 1800) cysticerci.
It has been studied what type (or types) of malic enzyme is present in the mitochondria of Taenia crassiceps cysticerci. Only NADP-dependent malic enzyme (EC 1.1.1.40) was demonstrated. The method of isoelectric focusing was used for the detection of isoenzymes of malic enzyme and malate dehydrogenase in the cytoplasm and mitochondria of T. crassiceps cysticerci. The mitochondria contain 5 isoenzymes of malic enzyme and 5 isoenzymes of malate dehydrogenase. The cytoplasm contains 7 isoenzymes of malic enzyme (3 of them are identical with those from the mitochondria) and 7 isoenzymes of malate dehydrogenase (5 of them are identical with those from the mitochondria). pI values of all isoenzymes were assessed.
Institute of Parasitology, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
J
J
Zenka
P
P
Kopácek
N
N
Vokurková
D000818:Animals; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000201:enzymology; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis*; D008928:Mitochondria / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D013621:Taenia / Q000201:enzymology* / Q000648:ultrastructure
D007527:Isoenzymes; D009243:NAD; D009249:NADP; D008291:Malate Dehydrogenase