Selenium deficiency, endurance exercise capacity, and antioxidant status in rats.
Increased O2 metabolism imposed by physical exercise is likely to augment the production of active O2 species that have been shown to react with lipids, proteins, and DNA. Antioxidants and antioxidant enzymes, such as the selenium enzyme glutathione peroxidase, minimize or prevent such potentially toxic reactions. This study shows that selenium deficiency decreases glutathione peroxidase activity in liver and muscle (less than 80%, P less than 0.001), increases total glutathione in liver, muscle, and plasma (P less than 0.05) and increases muscle cytochrome oxidase activity, and ubiquinone content (P less than 0.05) but has no effect on endurance capacity. Exercise to exhaustion resulted in a significant (P less than 0.001) elevation of total and oxidized glutathione (GSSG) and a significant (P less than 0.05) decrease of vitamin E in plasma of control and selenium-deficient rats. Acute exercise also increased tissue GSSG levels in both control and selenium-deficient groups of rats. Hence, despite a large depletion of selenium-deficient glutathione peroxidase, pronounced oxidation of glutathione to GSSG can be produced by the increased oxidative metabolism during physical exercise. The results suggest that the residual glutathione peroxidase activity is sufficient to detoxify hydroperoxides in exercising selenium-deficient animals and to prevent the impairment of endurance capacity.
Lang
J K
JK
Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Gohil
K
K
Packer
L
L
Burk
R F
RF
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008099:Liver / Q000032:analysis / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000032:analysis / Q000201:enzymology; D010807:Physical Endurance; D005082:Physical Exertion; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000172:deficiency*; D014451:Ubiquinone / Q000032:analysis; D014810:Vitamin E / Q000032:analysis
D000975:Antioxidants; D014451:Ubiquinone; D014810:Vitamin E; D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium