Interrenal secretion of corticosteroids and plasma cortisol and cortisone concentrations after acute stress and during seawater acclimation in juvenile coho salmon (Oncorhynchus kisutch).
We determined the major corticosteroids secreted by interrenal tissue and those present in plasma of juvenile coho salmon (Oncorhynchus kisutch). Incubation medium of interrenal tissue, unstimulated or stimulated with exogenous ACTH in vitro, and plasma of resting or acutely stressed salmon were extracted and qualitatively analyzed for steroid composition using high-performance liquid chromatography. Concentrations of plasma cortisone and cortisol following an acute handling stress or exposure to seawater were quantitatively measured by radioimmunoassay (RIA). Cortisol was the only corticosteroid detected by HPLC in media after incubation of interrenal tissue in the absence or presence of ACTH in vitro. However, both cortisone and cortisol were detected by HPLC in plasma sampled 1 hr after fish were acutely stressed by handling. Stress and seawater acclimation produced marked elevations in plasma levels of both steroids as determined by RIA and also resulted in long-lasting changes in the plasma cortisone:cortisol ratios. In resting fish, cortisone concentrations were similar or higher than cortisol levels. We concluded that cortisol is the primary steroid secreted by the interrenal tissue of coho salmon, and that plasma cortisone arises primarily from the peripheral conversion of cortisol to cortisone. The relatively high levels of cortisone in resting fish and its increase following stress and seawater acclimation suggest the possibility of a biologically significant role for this hormone.
Patiño
R
R
Oregon Cooperative Fishery Research Unit, Oregon State University, Corvallis 97331.
Redding
J M
JM
Schreck
C B
CB
D000064:Acclimatization; D000305:Adrenal Cortex Hormones / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003348:Cortisone / Q000378:metabolism*; D006854:Hydrocortisone / Q000097:blood*; D007668:Kidney / Q000378:metabolism; D011863:Radioimmunoassay; D012474:Salmon / Q000502:physiology*; D012623:Seawater; D013312:Stress, Physiological / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D003348:Cortisone; D006854:Hydrocortisone