Damage to DNA concurrent with lipid peroxidation in rat liver slices.
Lipid peroxidation and DNA damage were evaluated in liver slices incubated for 2 h at 37 degrees C with 1 mM-t-butyl hydroperoxide (t-BOOH), 1 mM-BrCCl3 or 50 microM-ferrous iron. t-BOOH induced the greatest amount of damage to DNA and increased the production of thiobarbituric acid-reactive substances (TBARS). Both phenomena depended on the incubation time. Ferrous iron induced both DNA damage and TBARS production, and BrCCl3 did not induce significant DNA damage and was the weakest TBARS inducer. Butylated hydroxytoluene at 1 mM inhibited both DNA damage and TBARS production. DNA damage and lipid peroxidation in liver slices were correlated, indicating that these events were concurrent.
Fraga
C G
CG
Department of Food Science and Technology, University of California, Davis, 95616.
Tappel
A L
AL
D000818:Animals; D001975:Bromotrichloromethane / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D004249:DNA Damage; D005296:Ferrous Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates / Q000096:biosynthesis; D020122:tert-Butylhydroperoxide
D005296:Ferrous Compounds; D010545:Peroxides; D013858:Thiobarbiturates; D002084:Butylated Hydroxytoluene; D020122:tert-Butylhydroperoxide; D001975:Bromotrichloromethane; C029684:thiobarbituric acid