Production of mycotoxins by Fusarium species isolated in Germany. 2. Time course of deoxynivalenol and 3-acetyldeoxynivalenol formation by Fusarium graminearum in different liquid media.
Several semisynthetic liquid media were examined for the large-scale production of deoxynivalenol (DON) und 3-acetyldeoxynivalenol (AcDON) by Fusarium graminearum 183. Only in three of the eight media used could high toxin yields of DON and AcDON be detected. The maximum levels of DON in a medium according to Miller were 3 mg/l and of AcDON 32 mg/l. In glucose-yeast extract-peptone (GYEP) medium containing 1% glucose, the AcDON concentrations reached 33 mg/l and the DON yields were 19 mg/l. In a rice flour liquid medium, however, the mean levels of AcDON and DON increased to 170 mg/l and 9 mg/l, respectively. The maximum amounts observed were 480 mg/l for AcDON and 65 mg/l for DON. The addition of trifluoracetic acid sodium salt or malonic acid, which are suggested to cause an accumulation of acetyl-CoA by inhibiting enzymes of the tricarboxylic acid cycle, did not stimulate the toxin formation.
Bayerische Landesanstalt für Bodenkultur und Pflanzenbau, Freising, Federal Republic of Germany.
M
M
Schuster
J
J
Lepschy
A
A
Biber
G
G
Engelhardt
P R
PR
Wallnöfer
D003470:Culture Media; D005433:Flour / Q000032:analysis; D005516:Food Microbiology; D005670:Fusarium / Q000378:metabolism*; D005860:Germany, West; D007700:Kinetics; D009183:Mycotoxins / Q000096:biosynthesis*; D012717:Sesquiterpenes / Q000096:biosynthesis*; D013997:Time Factors; D014255:Trichothecenes / Q000096:biosynthesis*
D003470:Culture Media; D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; C038972:acetyldeoxynivalenol; C007262:deoxynivalenol