[Radioimmunological analysis of 17 beta-estradiol and the effect of physical exertion on its levels in human blood].
Radioimmunoassay (RIA) for estradiol-17 beta (E2) in blood serum was developed. Antiserum against E2 was raised by means of rabbit immunization with 6-(O-carboxymethyl)oxime E2-BSA. Cross reactions of antiserum were studied with 22 natural and synthetic steroids. Antiserum was used at final dilution 1: 75,000. The sensitivity of RIA was 3 pg/tube, the detection efficiency was 83-120%, when 25-800 pg/ml E2 was added. The variation coefficients were 7.8% and 13% (intraassay and interassay, respectively). As shown by the RIA the intensive physical exercises resulted in the increase of E2 concentration by 26% in male athletes blood serum.
Morozov
V I
VI
Priiatkin
S A
SA
Isakova
T I
TI
Lushchitskaia
I M
IM
Rogozkin
V A
VA
D000293:Adolescent; D000328:Adult; D004958:Estradiol / Q000097:blood*; D006801:Humans; D008297:Male; D005082:Physical Exertion; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values
D004958:Estradiol
Metod radioimmunnogo analiza éstradiola-17 beta i vliianie fizicheskoĭ nagruzki na ego soderzhanie v krovi liudeĭ.