Low sensitivity of cultured human young adult and adult gingival fibroblasts to fluoride. I. Relation to doubling time.
Sensitivity to fluoride of human gingival fibroblasts derived from six different normal tissues (donor age; 17-36 years, male and female) and a foetal tissue were studied by measuring cell growth and protein synthesis in vitro. Fibroblasts derived from four different human foetal lungs were also studied in order to compare them with the gingival cells. Two cell lines of gingival and lung fibroblasts were derived from the same foetus. All of the young adult and adult gingival cells grew slowly in the medium containing 1.32 mM fluoride, while foetal cell growth, including the foetal gingival cells, was completely inhibited. Both foetal cultures had faster cell replication rates and greater protein synthesis rates than those of the young adult or adult cultures. The fluoride sensitivity was lower in all young adult and adult gingival fibroblasts than in the foetal cells. However, "aged" foetal cultures obtained by serial passage showed lower fluoride sensitivity. These "aged" cells also had prolonged generation times. These findings indicate that although the mechanism is unclear, the low fluoride sensitivity of the young adult and adult gingival fibroblasts observed in this study are likely to be related to their slower rate of cell growth.
Department of Preventive and Community Dentistry, School of Dentistry Nippon Dental University, Tokyo, Japan.
T
T
Sato
A
A
Yagori
M
M
Niwa
D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005459:Fluorides / Q000494:pharmacology*; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy
D005459:Fluorides