Elevated c-myc expression in progeria fibroblasts.
Protooncogene expression was determined in cultured human fibroblasts derived from donors diagnosed with Hutchinson-Gilford Syndrome (Progeria). An increased level of c-myc expression was noted in progeria cells compared to normal parental controls and unrelated, unaffected, age-matched controls. In two progeria cultures a 150% increase in c-myc expression was observed, while a third progeria culture, subsequently identified as a non-classic progeria showed a 45% increase in c-myc expression. Southern blot analysis did not indicate an amplification or translocation of the c-myc gene. No significant differences were found in the expression of the c-erbB or c-src protooncogenes.
Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079.
K D
KD
Nakamura
A
A
Turturro
R W
RW
Hart
D002478:Cells, Cultured; D005347:Fibroblasts / Q000032:analysis; D005786:Gene Expression Regulation; D006801:Humans; D009857:Oncogenes; D011371:Progeria / Q000235:genetics*; D011519:Proto-Oncogenes