[Amino acid analysis of proteins and physiological fluids in a single system of sodium-citrate buffer].
Programmes for automatic analysis of protein and peptide hydrolysates and physiological fluids in a single system of sodium citrate buffers are proposed. Retention time values of 59 ninhydrin-positive substances, including nonprotein amino acids, amino sugars (muramic acid, glucosamine, galactosamine, mannosamine and fucosamine) and corresponding aminopolyols, as well as three cysteine derivatives, viz. cysteic acid, S-carboxymethylcysteine and S-2-(pyridyl-4)ethylcysteine, have been determined. Several examples illustrate an increase of the resolution simply by change of the temperature programme of the column and (or) elution time.
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Rodionov
D000596:Amino Acids / Q000032:analysis*; D000606:Amino Sugars / Q000032:analysis; D002021:Buffers; D002951:Citrates; D009555:Ninhydrin; D011492:Protein Hydrolysates / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012964:Sodium
D000596:Amino Acids; D000606:Amino Sugars; D002021:Buffers; D002951:Citrates; D011492:Protein Hydrolysates; D011506:Proteins; D012964:Sodium; D009555:Ninhydrin
Aminokislotnyĭ analiz belkov i fiziologicheskikh zhidkosteĭ v odnoĭ sisteme natriĭ-tsitratnykh buferov.