Increased angiotensin II receptors in brain nuclei of DOCA-salt hypertensive rats.
We analyzed angiotensin II (ANG II) receptors by in vitro autoradiography in selective brain nuclei of control, salt-treated (1% NaCl in drinking water), deoxycorticosterone acetate (DOCA)-treated (DOCA pivalate, 25 mg/kg sc weekly), and DOCA-salt-treated (DOCA + salt treatments) uninephrectomized male Wistar-Kyoto rats. After 4 wk of treatment, only the DOCA-salt group developed hypertension. ANG II binding increased in median preoptic nucleus and subfornical organ of salt- and DOCA-treated rats. DOCA-treated rats also showed increased ANG II binding in paraventricular nucleus. DOCA-salt-treated rats showed higher ANG II binding in nucleus of the solitary tract and area postrema, as well as in the areas mentioned before. Although salt and/or DOCA treatments alone increased ANG II receptors in some brain nuclei, after combined DOCA-salt treatment there was significantly higher ANG II binding in all areas, except the median preoptic nucleus. These results suggest that increased ANG II receptors in selected brain areas may play a role in the pathophysiology of mineralocorticoid-salt experimental hypertension.
Unit on Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, Maryland 20892.
J S
JS
Gutkind
M
M
Kurihara
J M
JM
Saavedra
D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001794:Blood Pressure; D001921:Brain / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D003900:Desoxycorticosterone; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D009830:Olfactory Bulb / Q000378:metabolism; D009928:Organ Specificity; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D011301:Preoptic Area / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D011945:Receptors, Angiotensin / Q000378:metabolism*; D012016:Reference Values; D012982:Sodium, Dietary; D013356:Subfornical Organ / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*
D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin; D012982:Sodium, Dietary; D000804:Angiotensin II; D003900:Desoxycorticosterone