Comparison of pteroylpolyglutamate hydrolase (folate conjugase) from porcine and human intestinal brush border membrane.
1. A comparative study of pteroylpolygluatamte hydrolase (folate conjugase) of brush border membrane vesicles from human and porcine intestine was conducted. 2. The enrichment of conjugase activity during membrane isolation was 5-fold greater for the human than the pig. 3. Porcine and human conjugases exhibited similar Km values and could completely hydrolyze pteroyltriglutamate (PteGlu3) to PteGlu1 via an exohydrolytic process. 4. Pteroic acid, PteGlu1 and anionic polysaccharides did not inhibit human or porcine conjugase. 5. Apparent mol. wts for detergent-enzyme complexes were 237,000 (pig) and greater than 500,000 (human). 6. These results indicate similar kinetic properties and mode of action but differences in physical behavior between the intestinal brush border folate conjugases of human and pig.
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611.
J F
JF
Gregory
S L
SL
Ink
J J
JJ
Cerda
D000818:Animals; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D006801:Humans; D007583:Jejunum / Q000201:enzymology*; D007700:Kinetics; D008871:Microvilli / Q000201:enzymology; D008970:Molecular Weight; D011624:Pteroylpolyglutamic Acids; D013045:Species Specificity; D013552:Swine; D011623:gamma-Glutamyl Hydrolase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*
D011624:Pteroylpolyglutamic Acids; C042658:triglutamate folate; D011623:gamma-Glutamyl Hydrolase; D003546:Cysteine Endopeptidases