The effect of human serum on the binding activity of radiolabelled monoclonal antibodies.
Three murine monoclonal antibodies (MoAbs), MBr1 and MOv2 of IgM isotype and MOv8 of IgG isotype, with restricted reactivity for breast or ovarian carcinomas, were labelled with 125I in the perspective of obtaining specific and stable radioimmunopharmaceutical reagents. The radiolabelled MoAbs were analyzed for their "in vitro" stability in human blood. They were incubated at 37 degrees C for various lengths of time in human or, as a control, in murine blood and their binding capacity was evaluated by solid-phase RIA and compared with that obtained after incubation with buffer. In human blood, serum and plasma, but not with other components such as erythrocytes, leukocytes, HSA and IgG, the MoAbs revealed a loss of binding reactivity which was marked and constant for the IgM MoAbs, and only occasional for the IgG MoAb. In murine serum the decrease was not so rapid. The same change in the binding capacity was observed when the MoAbs were labelled with 3H or 35S, excluding the involvement of dehalogenating mechanisms. In the perspective of using MoAbs for intracavity therapy the effect of ascitic or pleural fluids on their binding activity was also evaluated. The inhibition of the binding reactivity was not as evident and was not related to the MoAb isotype.
Division of Experimental Oncology E, Istituto Nazionale Tumori, Milan, Italy.
S
S
Camagni
S
S
Canevari
M
M
Ripamonti
D
D
Mezzanzanica
R
R
Orlandi
M I
MI
Colnaghi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000627:therapeutic use; D001790:Blood Physiological Phenomena; D004355:Drug Stability; D006801:Humans; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D010996:Pleural Effusion / Q000503:physiopathology
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes