Value of a new screening test for respiratory allergy.
Phadiatop (Pharmacia) is a new in vitro screening test for respiratory allergy which detects in serum the presence of specific IgE to a mixture of common inhalant allergens; results are either positive or negative. Among 100 patients suffering from rhinitis and/or asthma, investigated with 15 skin prick tests (SPT) and RAST for frequent allergens, the Phadiatop was positive in 63: all 63 had at least one positive SPT and RAST (specificity: 100%). Conversely, ther were 67 patients who had a positive RAST for at least one allergen: among these 67 the total IgE concentration in serum was high (greater than 100 kU/l) in 43 (64%) and the Phadiatop was positive in 63 (94%). Phadiatop was negative in serum obtained from 10 cord bloods and 10 non-allergic subjects (disease-free, 15 negative SPT and RAST). Results of the radio-immunoassay originally proposed by the manufacturer were similar to those obtained with a modified enzymo assay using a fluorometric substrate. In the prediction of respiratory allergy, Phadiatop was found to be specific and more accurate than the determination of total IgE in serum.
Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
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D000293:Adolescent; D000328:Adult; D000485:Allergens; D001249:Asthma / Q000175:diagnosis; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis*; D007073:Immunoglobulin E / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011852:Radioallergosorbent Test / Q000379:methods*; D011863:Radioimmunoassay / Q000379:methods*; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis
D000485:Allergens; D007073:Immunoglobulin E