Somatotopic organization of the dorsal horn in the lumbosacral enlargement of the spinal cord in the neonatal cat.
The somatotopic organization of the light touch receptive fields of single unidentified dorsal horn neurons in the lumbosacral spinal cord has been studied in the neonatal cat anesthetized with chloralose. Satisfactory recordings were obtained from single dorsal horn neurons in kittens aged 3-6 days. Reconstruction of recording tracks from pontamine blue dye spots and comparisons of the depths of recording sites with Nissl-stained sections of cord showed that most single-unit recordings were obtained from laminae III and IV of Rexed. In animals of all ages neurons were found which responded briskly to light cutaneous mechanical stimulation. Their receptive fields varied widely in size, being smallest on the distal digits and largest on proximal skin. Receptive field areas were similar in proportion to the size of the hindlimb to those seen in the equivalent region in the adult cat. Because of the shape of the dorsal horn and the relatively narrow dorsal columns in neonatal kittens it proved difficult to locate units with receptive fields on proximal skin. Nevertheless the main features of the somatotopic organization of the dorsal horn were similar to those in the adult cat. Thus the somatotopic map of the kitten showed a medial representation of glabrous skin that was bounded laterally by the representation of the hairy skin of the toes. Proximal skin was represented in the lateral parts of the dorsal horn, a region which was not easily accessible for microelectrode recording. The individual toes were represented in a rostral to caudal sequence such that toe 2 was represented rostrally and toe 5 caudally. Around the toe representation the medial surface of the foot was represented rostrally, the ventrolateral surface caudally, and the dorsal surface laterally. The results indicate that the mature organization of light touch receptive fields of dorsal horn neurons in the lumbosacral cord of the cat is already largely present at birth.
Department of Anatomy, University of Queensland, St. Lucia, Australia.
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P
Wilson
P J
PJ
Snow
D000200:Action Potentials; D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology*; D002415:Cats; D005727:Ganglia, Spinal / Q000033:anatomy & histology*; D006614:Hindlimb / Q000294:innervation; D008161:Lumbosacral Region / Q000294:innervation; D009475:Neurons, Afferent / Q000502:physiology; D011984:Sensory Receptor Cells / Q000033:anatomy & histology; D012867:Skin / Q000294:innervation; D013116:Spinal Cord / Q000033:anatomy & histology*