Identification of a developmentally regulated calcium-binding protein in Trypanosoma brucei.
Calcium ions mediate cellular activity by binding to specific cellular proteins. The following study systematically examines the cellular complement of calcium-binding proteins in different cell fractions and life cycle stages of Trypanosoma brucei. Using a 45Ca-gel overlay procedure, eight calcium-binding proteins were consistently observed. The majority of proteins were cytosolic (84, 70, 64, 22, and 15 kd) while the remainder (55, 46, and 29 kd) were particulate. Although calmodulin was detected amongst the calcium-binding proteins, it did not represent the majority of calcium-binding activity. Of special interest was the 46 kd calcium-binding protein which was associated with 3-fold more calcium in cultured procyclic forms than in slender bloodstream forms. By contrast, promastigote forms of Leishmania mexicana did not contain the 46 kd calcium-binding protein. These data suggest that responsiveness to calcium signals may vary during the trypanosome life cycle as a result of changes in the cellular complement of calcium-binding proteins.
Department of Biology, Southern Methodist University, Dallas, Texas 75275.
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D000818:Animals; D001345:Autoradiography; D002135:Calcium-Binding Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D014346:Trypanosoma brucei brucei / Q000032:analysis* / Q000254:growth & development
D002135:Calcium-Binding Proteins