Mutagenesis of OA-6129 carbapenem-producing blocked mutants and the biosynthesis of carbapenems.
Streptomyces fulvoviridis A933 17M9 1501 is an A933 acylase-defective mutant derived from S. fulvoviridis A933 17M9 and thus produces the OA-6129 group of carbapenems and carbapenams. By further mutation of mutant 1501, 4 types of mutants (OA-6129 A + B1 + B2 producers; OA-6129 A + B2 producers; an OA-6129 A producer; non-producers) were obtained. The second type of mutant strains 4N 3607, 5NA 3949-40 and 5NE 252 proved useful for the fermentative production of carbapenem OA-6129 B2. These results of mutagenesis demonstrated that the sequence of carbapenem bioconversion in the horizontal route was hydroxylation at C-8----isomerization at C-6----sulfation at C-8 hydroxyl.
Kojima
I
I
Sanraku Incorporated, Central Research Laboratories, Fujisawa, Japan.
Fukagawa
Y
Y
Okabe
M
M
Ishikura
T
T
Shibamoto
N
N
D009154:Mutation; D013302:Streptomyces / Q000378:metabolism*; D013845:Thienamycins / Q000096:biosynthesis*
D013845:Thienamycins; C036499:OA 6129C; C036497:OA 6129A; C036498:OA 6129B(1)