Measurement of aromatase activity in the brain of the African catfish, Clarias gariepinus--a comparison of two assay methods.
In the brain of the African catfish aromatase activity was demonstrated with two different methods using [7-3H]androstenedione and [19-3H]androstenedione as substrates. Kinetic analysis of estrogen formation following incubations of a cell-free fraction of brain homogenates showed that the apparent Km is the same for both substrates (0.03 micro M), but the Vmax is smaller with [19-3H]androstenedione as substrate. This indicates a so-called isotope effect. A time course study showed that after an incubation of 4 hr the aromatase activity is still linearly time dependent. Comparing the amount of estrogens formed from both precursors showed that the value of the isotope effect is 3.4 Incubation of symmetric punches of telencephalon and diencephalon with the two substrates likewise showed an isotope effect of 3.4
Timmers
R J
RJ
Department of Experimental Zoology, University of Utrecht, The Netherlands.
Lambert
J G
JG
D000735:Androstenedione; D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D002397:Catfishes / Q000378:metabolism*; D004967:Estrogens / Q000096:biosynthesis; D007700:Kinetics; D008297:Male; D013379:Substrate Specificity; D014316:Tritium
D004967:Estrogens; D014316:Tritium; D000735:Androstenedione; D001141:Aromatase