Composition of pure pancreatic juice in normal human subjects and in pancreatic disease.
Pure pancreatic juice (PPJ) was collected at endoscopic retrograde cholangio-pancreatography from patients with or without pancreatic disease. Two-dimensional polyacrylamide gel electrophoresis of PPJ proteins demonstrated a wide heterogeneity of proteins, differing in molecular weight and isoelectric points. There was one major difference in the protein patterns between normals and those with chronic pancreatitis. A glycoprotein has been identified and shown to be present in 28/30 patients without pancreatic disease, but absent in 11 or greatly reduced in 8 of 19 patients with mild pancreatitis and absent in all 13 patients with moderate or marked pancreatitis and both patients with pancreatic carcinoma.
Department of Medicine, St. James's University Hospital, Leeds, UK.
J K
JK
Jutley
M E
ME
Denyer
J
J
Kelleher
C J
CJ
Mitchell
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002908:Chronic Disease; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010189:Pancreatic Juice / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010195:Pancreatitis / Q000378:metabolism*