Regulation of hepatitis B virus S gene promoter in transfected cell lines.
Hepatitis B virus (HBV) contains an enhancer element that activates the viral core and X gene promoters. To investigate the transcriptional regulation of the viral S gene promoter, we transfected SK-Hep1 cells with circularized forms of HBV DNAs and their enhancerless mutants. We have found that expression of the S gene, determined by measurement of the appearance of HBsAg in the media and by RNA analysis, is to a large extent enhancer-dependent. This observation was further confirmed by analysis of a series of plasmids containing the chloramphenicol acetyl-transferase (CAT) gene under the control of the S gene promoter and the HBV enhancer element. Interestingly, in contrast to its behavior in SK-Hep1 cells, the S gene promoter is highly active in Alexander cells, in the absence of the enhancer element. This implies that activity of the S gene promoter is cell-type specific.
Department of Virology, Weizmann Institute of Science, Rehovot, Israel.
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Faktor
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De-Medina
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Shaul
D000123:Acetyltransferases / Q000235:genetics; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D006514:Hepatitis B Surface Antigens; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase