Quantitative method for determination of hemolytic activity of Clostridium septicum toxin.
A quantitative method for titration of hemolytic activity of Clostridium septicum toxin was established. No linear dose-response line was obtained between the toxin dose and hemolysis ratio. Logit or Probit transformation of the hemolysis ratio changed this log-dose response curve into a linear line. We recommend a highly reproducible and accurate method based on parallel line assay using a well-standardized reference toxin for determination of hemolytic activity. The method consists of simultaneous titrations of the hemolysis ratios with each sample and a reference toxin, Logit or Probit transformation of the hemolysis ratio, and expression of the activity in the relative hemolytic value.
Chiba Serum Institute.
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S
Takano
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H
Kondo
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000633:toxicity*; D003013:Clostridium; D004906:Erythrocyte Count; D006460:Hemolysin Proteins / Q000032:analysis*; D006461:Hemolysis / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D011817:Rabbits
D001427:Bacterial Toxins; D006460:Hemolysin Proteins