High-performance liquid chromatographic method for determining trichothecene mycotoxins by post-column fluorescence derivatization.
The method described here is based on a separation of deoxynivalenol, nivalenol and fusarenon-X on a C18 column using aqueous acetonitrile, and successive post-column fluorescence derivatization involving an alkaline decomposition to form formaldehyde and modified Hantzsch reaction with methyl acetoacetate and ammonium acetate (lambda ex = 370 nm and lambda em = 460 nm). By this method, 5-10 ng of the standard trichothecenes could be determined. By employing a clean-up procedure with a florisil column and a Sep-Pak CN cartridge, 61.4-96.9% recoveries were obtained for deoxynivalenol and nivalenol added to corn, wheat and barley at concentration levels of 0.05-1 ppm.
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
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Sano
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Matsutani
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M
Suzuki
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S
Takitani
D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000032:analysis; D009183:Mycotoxins / Q000032:analysis*; D012717:Sesquiterpenes / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014255:Trichothecenes / Q000032:analysis*; D003313:Zea mays / Q000032:analysis
D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes