An immunogenic complex of alkali-treated lipid A and methylated bovine serum albumin.
Alkali-treated lipid A prepared by hydrolysis of lipid A in 0.25 N NaOH (1 h, 56 degrees C) formed an insoluble complex with methylated bovine serum albumin (MBSA) in aqueous medium. At a 1/1 (w/w) lipid A/MBSA ratio, nearly all lipid A was insolubilized. The complex was assumed to be ionic in nature. On intravenous immunization of rabbits with the complex, antibodies directed against lipid A were elicited. The antibody titres ranged from 1/256 to 1/2,048, as demonstrated by the passive haemolysis test, and proved to be mainly of the IgM class.
Central Laboratory of Genetic Engineering, Sofia.
M
M
Eskenazy
R
R
Tekelieva
R
R
Ivanova
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000302:isolation & purification; D008050:Lipid A / Q000276:immunology*; D046911:Macromolecular Substances; D008745:Methylation; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007136:Immunoglobulins; D008050:Lipid A; D046911:Macromolecular Substances; D012710:Serum Albumin, Bovine