Lipocytes and transitional cells in alcoholic liver disease: a morphometric study.
Lipocytes and transitional cells in alcoholic liver disease were analyzed by quantitative morphometry in liver biopsy specimens of 17 alcoholic patients. In fatty livers, 93% of the perisinusoidal cells were lipocytes with a volume of lipid droplets occupying more than 20% of the individual cell volume. In fatty livers with perivenular fibrosis, 83% of the cells were lipocytes and 17% were transitional cells with lipid droplets of less than 20% of the cell volume. In cirrhosis, 45% of the cells were lipocytes and 55% of the cells were transitional cells. Thus, the process of hepatic fibrosis was associated with a shift from lipocytes to transitional cells. The percentage of transitional cells (expressed as total perisinusoidal cells) was 5.1% in fatty livers, 16.5% in fatty livers with perivenular fibrosis (not significantly different when compared to fatty livers) and 59.7% in cirrhotic livers (p less than 0.025) when compared to fatty livers or fatty livers with perivenular fibrosis). The number of transitional cells per 100 hepatocytes was estimated to be 0.5 in fatty livers, 1.3 in fatty livers with perivenular fibrosis and 2.1 in cirrhotic livers. The appearance of transitional cells was associated with a corresponding decrease in the number of lipocytes, supporting the hypothesis that transitional cells were derived from lipocytes. In comparison to lipocytes, the surface area of transitional cells (as measured by digitized morphometry) was 30 to 46% smaller (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Mak
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KM
Alcohol Research and Treatment Center, Bronx Veterans Administration Medical Center, New York 10468.
Lieber
C S
CS
D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology*; D001706:Biopsy; D002452:Cell Count; D005235:Fatty Liver, Alcoholic / Q000378:metabolism / Q000473:pathology; D005355:Fibrosis; D006519:Hepatitis, Alcoholic / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008104:Liver Cirrhosis, Alcoholic / Q000378:metabolism / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron