The bulk of ATP is associated to proteins in the living cell: a release kinetics study.
The analysis of the nonionic detergent induced release kinetics of intracellular components is a promising approach to obtain information on the possible molecular interactions inside the living cell. The molecular interactions of ATP were studied by monitoring the nonionic detergent-induced release kinetics of ATP from cultured human cells. The observed rapid mobilization rate in the presence of Triton X 100 and the retarded release in the presence of Brij 58 were in good agreement with the detergent-induced release kinetics of proteins and K+ reported earlier. These data are consistent with the idea that the bulk of ATP is associated to the protein macromolecules of the living cell.
Koszegi
T
T
Department of Clinical Chemistry, University Medical School, Pecs, Hungary.
Kellermayer
M
M
Berenyi
E
E
Jobst
K
K
Hazlewood
C F
CF
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D003902:Detergents / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
D003902:Detergents; D011506:Proteins; D000255:Adenosine Triphosphate