TDPAC studies of the metal-binding sites in serum transferrin: comparison between 181Hf-labeled human- and rat-serum transferrin.
The binding of hafnium to human serum transferrin was studied using the time differential perturbed angular correlation (TDPAC-) technique. The samples were prepared in vitro by adding 181Hf-NTA solution to human serum. Two specific electric quadrupole interactions were observed, which correspond to two well-defined binding configurations. Their relative intensities depend on the pH, salt- and hafnium-concentrations, and on the incubation time. The present data may be compared with the results of a previous rat serum study, where the hafnium binding to transferrin behaved rather similarly. Small but significant differences, however, can be deduced from the TDPAC-parameters for these human and rat transferrin species. For either binding configuration, the electric field gradient (EFG) is slightly higher in the case of rat transferrin. The most characteristic difference, however, concerns the asymmetry parameter eta 2 of the second binding configuration, which is about 10% smaller for rat serum transferrin. The TDPAC-technique might be used as a sensitive and reliable analytical method to study the metal-binding sites of different transferrin species.
Appel
H
H
Department of Applied Chemistry, University of Wales, Cardiff, U.K.
Duffield
J
J
Taylor
D M
DM
Then
G M
GM
Thies
W G
WG
D000818:Animals; D006195:Hafnium / Q000097:blood; D006801:Humans; D008670:Metals / Q000097:blood*; D011485:Protein Binding; D011868:Radioisotopes; D051381:Rats; D013045:Species Specificity; D013057:Spectrum Analysis; D014168:Transferrin / Q000378:metabolism*
D008670:Metals; D011868:Radioisotopes; D014168:Transferrin; D006195:Hafnium