Factors affecting malate dehydrogenase activity in freezing-thawing processes.
Malate dehydrogenases from several sources show different behaviour when frozen-thawed in 100 mM sodium phosphate buffer, pH 7.4, containing chaotropic ions. The effects produced by the addition of various metabolites, protein concentration and buffer medium used on the loss of activity induced by the freezing-thawing process are reported. The major part of the loss of activity is caused by the formation of "wrong" aggregates of high mol. wt.
Domènech
C
C
Department of Biochemistry and Physiology, University of Barcelona, Spain.
Bozal
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X
Mazo
A
A
Cortés
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A
Bozal
J
J
D000818:Animals; D000838:Anions; D002021:Buffers; D002645:Chickens; D002850:Chromatography, Gel; D003067:Coenzymes; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005615:Freezing; D006168:Guinea Pigs; D008244:Lysophosphatidylcholines; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008970:Molecular Weight; D010710:Phosphates
D000838:Anions; D002021:Buffers; D003067:Coenzymes; D008244:Lysophosphatidylcholines; D010710:Phosphates; D008291:Malate Dehydrogenase; C018279:sodium phosphate