The effect of the specific PAF antagonist BN 52021 and the calcium blocker diltiazem on PAF induced arrhythmogenicity.
Platelet-activating factor (PAF) in a concentration of 10(-11) mole per animal decreased the threshold doses for onsets of arrhythmia, ventricular flutters and fibrillation in ouabain induced arrhythmia in guinea-pigs in a statistically significant manner. The specific PAF antagonist BN 52021 (20 mg/kg, orally) completely inhibited the PAF effect for all types of arrhythmia, while the Ca2+ antagonistic drug diltiazem (0.1 mg/kg, i.v.) failed to counteract the PAF action. BN 52021 (20 mg/kg, per os) alone did not exert any effect on ouabain induced arrhythmia, but as expected, diltiazem (0.1 mg/kg, i.v.) showed antiarrhythmic effects. These results confirm the specific PAF antagonistic activity of BN 52021, its usefulness for PAF related cardiac rhythm disturbances and indicate that the method used could be a further useful tool to screen PAF antagonistic substances.
Dept. of Pharmacology, Martin Luther University, Halle, GDR.
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Riedel
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Braquet
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Mest
D000284:Administration, Oral; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000139:chemically induced* / Q000503:physiopathology; D004110:Diltiazem / Q000494:pharmacology*; D004224:Diterpenes; D046934:Ginkgolides; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D007783:Lactones / Q000008:administration & dosage / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D010042:Ouabain; C045856:ginkgolide B; D004110:Diltiazem