Selective medium for isolation and enumeration of Bifidobacterium spp.
A new method was developed for the isolation and enumeration of Bifidobacterium spp. from natural aquatic environments. The method was based on the utilization of a new medium, Bifidobacterium iodoacetate medium 25, and resuscitation techniques were used to isolate injured bifidobacteria. The new medium was tested with a nonselective reference medium on sewage and sewage-polluted surface waters. Relatively little colonial growth of any other bacterial genera occurred; when such colonies did grow, Bifidobacterium could be easily differentiated by its colonial morphology or, after Gram staining, by its typical bifidobacterial morphology.
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Spain.
F J
FJ
Muñoa
R
R
Pares
D001644:Bifidobacterium / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media; D012722:Sewage; D014871:Water Microbiology
D003470:Culture Media; D012722:Sewage