Totally automated liquid-chromatographic assay for cortisol and associated glucocorticoids in serum, with use of ASTED sample preparation.
We describe estimation of total cortisol in serum by HPLC. Incorporation of a new technique to totally automate sample preparation decreases costs. The benefits of HPLC ensure that interferences from other steroids are avoided. The method can also be used to estimate concentrations of other glucocorticoids in serum.
Biochemistry Department, Coventry and Warwickshire Hospital, U.K.
D C
DC
Turnell
J D
JD
Cooper
B
B
Green
G
G
Hughes
D J
DJ
Wright
D000312:Adrenal Hyperplasia, Congenital; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D003480:Cushing Syndrome / Q000097:blood / Q000188:drug therapy; D003956:Dialysis; D005938:Glucocorticoids / Q000097:blood*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008797:Metyrapone / Q000627:therapeutic use; D011786:Quality Control
D005938:Glucocorticoids; D006854:Hydrocortisone; D008797:Metyrapone