Dark-field electron microscopy of unstained biological materials embedded in Nanoplast.
Extremely thin sections of unstained materials (beef liver catalase, double-stranded calf thymus DNA, horse spleen ferritin and mammalian skeletal muscle), embedded in the water-soluble melamine resin Nanoplast FB101, were studied by dark-field electron microscopy and electron spectroscopic imaging. While ferritin molecules so recorded show 0.4 and 0.9 nm lattice fringes within the crystalline iron core, double-stranded DNA shows a helical repeat with a spacing of 3.4 nm. The gain in resolution of structural detail reported here is probably due mainly to the reduced section thickness as compared to traditional thin-sectioning techniques. As we reported earlier (Frösch & Westphal, 1984), melamine resins can be sectioned extremely thinly (less than 10 nm) and observed without a supporting film, making them especially suitable for dark-field electron microscopy.
Sektion Elektronenmikroskopie, Universität Ulm, F.R.G.
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Frösch
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Westphal
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Bauer
D000818:Animals; D004247:DNA / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D009132:Muscles / Q000648:ultrastructure; D051381:Rats; D013154:Spleen / Q000648:ultrastructure; D014021:Tissue Preservation / Q000379:methods; D014227:Triazines
D014227:Triazines; C050897:nanoplast; D004247:DNA