[The casein genes of Bos taurus. II. Isolation and characteristics of the beta-casein gene].
The expression of the casein genes in mammary gland cells is regulated by peptide and steroid hormones. To study underlying regulatory mechanisms, the bovine beta-casein gene was isolated and characterized from lambda bacteriophage bovine DNA library. The beta-casein gene is 8.6 kb long and is 7.8 times longer than the mature casein mRNA coded for by 9 exons. The genomic clones incorporate additional 8.5 and 4.5 kb of the 5'- and 3'-flanking regions. The nucleotide sequences of 5' and 3' ends of the beta-casein gene are determined. Conserved sequences identical or homologous to potential sites of binding with the nuclear factor CTF/NF-1, glucocorticoid and progesterone receptors were identified. The regulatory region of the casein gene contains two different TATA signals flanking the duplication site in the promoter region.
Gorodetskiĭ
S I
SI
Tkach
T M
TM
Kapelinskaia
T V
TV
D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005786:Gene Expression Regulation; D005796:Genes; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D051381:Rats
D002364:Caseins; D004247:DNA
Geny kazeinov Bos taurus. Soobshchenie II. Vydelenie i kharakteristika gena beta-kazeina.