Assessment of intestinal damage in rotavirus infected neonatal mice by a D-xylose absorption test.
A D-xylose absorption test has been standardized for use in newborn mice. It was used to measure small intestine dysfunction in neonate mice following infection with various isolates of rotaviruses. A xylose dose of 1 mg/g body weight was required to produce a maximum level of 100 micrograms/100 microliters of plasma 2 h after administration of D-xylose. The mice inoculated with rotavirus absorbed significantly less D-xylose compared with uninoculated control mice. A micromethod is described which proved to be suitable to quantitate D-xylose for determination of absorptive function of the small intestine in small animals such as newborn mice.
Ijaz
M K
MK
Veterinary Infectious Disease Organization (VIDO), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Sabara
M I
MI
Frenchick
P J
PJ
Babiuk
L A
LA
D000818:Animals; D007408:Intestinal Absorption; D007410:Intestinal Diseases / Q000382:microbiology* / Q000503:physiopathology; D051379:Mice; D012016:Reference Values; D012400:Rotavirus Infections / Q000503:physiopathology*; D013045:Species Specificity; D014994:Xylose / Q000378:metabolism*
D014994:Xylose