Amplification and analysis of DNA sequences in single human sperm and diploid cells.
The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.
Li
H H
HH
Department of Biological Sciences, University of Southern California, Los Angeles 90089-0371.
Gyllensten
U B
UB
Cui
X F
XF
Saiki
R K
RK
Erlich
H A
HA
Arnheim
N
N
D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002875:Chromosomes; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D005914:Globins / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D011973:Receptors, LDL / Q000235:genetics; D011995:Recombination, Genetic; D013094:Spermatozoa / Q000648:ultrastructure*
D006683:HLA-DQ Antigens; D011973:Receptors, LDL; D005914:Globins; D004247:DNA; D004259:DNA-Directed DNA Polymerase