Urea determination in dialysis, based on a differential pH technique.
The application of a new technique, based on differential measurements of pH, to determine urea concentration in patients of a dialysis center, is reported. Urea in plasma, whole blood or dialysis fluids is measured by an enzymatic reaction, with urease; the procedure, requiring 10 microL of sample, is simple, fast and correlates well with a reference spectrophotometric method, in the 0-300 mg/dL concentration range, according to the equation y = 1.0291 X -0.0777; r = 0.9991; n = 73.
Servizio di Nefrologia e Dialisi, Ospedale dei Bambini Umberto I, Brescia, Italy.
D
D
Bonucchi
A
A
Castellani
G C
GC
Orlandini
M
M
Ripamonti
M
M
Luzzana
A
A
Mosca
D001322:Autoanalysis / Q000295:instrumentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D006435:Renal Dialysis; D014508:Urea / Q000032:analysis* / Q000097:blood; D014510:Urease
D014508:Urea; D014510:Urease