Rapid procedure for determination of nicarbazin residues in chicken tissues.
A procedure is described for the quantitation of nicarbazin residues in chicken tissues. The method includes extraction of tissue with chloroform-ethyl acetate-dimethyl sulfoxide (50 + 50 + 0.8), adsorption on neutral alumina, and subsequent elution of the residues with methanol-pH 6.0 phosphate buffer (1 + 1). Extracts are separated on a 15 cm, 5 micron C18 column with methanol-pH 6.0 phosphate buffer (6.5 + 3.5) as the mobile phase. The dinitrocarbanilide portion of the complex is detected and quantitated with an electrochemical detector in the reductive mode. Recoveries, based on dinitrocarbanilide, were greater than 95% in liver, breast, and thigh muscle tissues fortified with 0.25-8.0 ppm nicarbazin.
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U.S. Department of Agriculture, Eastern Regional Research Center, Philadelphia, PA 19118.
D000818:Animals; D002224:Carbanilides / Q000032:analysis*; D002645:Chickens; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D008099:Liver / Q000032:analysis; D009132:Muscles / Q000032:analysis; D009528:Nicarbazin / Q000032:analysis*; D012015:Reference Standards
D002224:Carbanilides; D009528:Nicarbazin