A temperature controlled chamber to allow observation and measurement of uptake of fluorochromes into live cells.
A temperature controlled cell chamber is described which permits phase-contrast and fluorescence microscopy of living cells. It is shown how the cellular uptake of fluorochromes can be investigated both photographically and photometrically. Some sample results are presented for the uptake of Evans Blue.
Payne
J N
JN
Department of Anatomy and Cell Biology, Sheffield University.
Cooper
J D
JD
MacKeown
S T
ST
Horobin
R W
RW
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005070:Evans Blue / Q000493:pharmacokinetics; D005347:Fibroblasts / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D013696:Temperature
D005456:Fluorescent Dyes; D005070:Evans Blue