[Comparison of different test systems for the radioimmunological determination of the hepatitis B virus surface antigen using monoclonal antibodies].
The sensitivity of test systems for radioimmunological detection of HBsAg prepared on the basis of polyclonal immunoglobulins, affinity polyclonal antibody (PCA) and monoclonal antibody (MCA) was compared. MCA ZhAK 22 and ZhAK 12 interacting with two different HBsAg determinants: general-type "a" and subtype "y", respectively, were used. The solid phase was sensitized with MCA of each clone or a mixture of MCA in concentration ratios of 1:1, 1:2, and 2:1. After sensitization of the solid phase and addition of HBsAg, 125I-labeled affinity PCA were added (in all tests). The radioimmunological test-system based on MCA ZhAK 22 and ZhAK 12 adsorbed on the solid phase at a 2:1 ratio was found the most sensitive: 0.5 ng, i.e. 4 times as high as that of the system prepared on the basis of PCA.
A S
AS
Shchegolev
A A
AA
Kushch
A M
AM
Momot
E P
EP
Shumaĭ
Iu S
IuS
Skoblov
D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000295:instrumentation* / Q000379:methods
D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens; D007457:Iodine Radioisotopes
Sravnenie razlichnykh test-sistem dlia radioimmunologicheskogo opredeleniia poverkhnostnogo antigena virusa gepatita B s ispol'zovaniem monoklonal'nykh antitel.