An immunocytochemical method for studying embryo cytokinetics.
The immunocytochemical detection by a monoclonal antibody of bromodeoxyuridine incorporated into S-phase cells allows the identification of proliferating cells. In this study we demonstrate that the labelling of embryo tissues is achieved by a single administration of BrdUrd to the mother after a 1 hour-labelling period. This simple and rapid technique facilitates the detection of proliferating cells within the embryo for the study of developing tissues and embryo cytokinetics.
Istituto di Istologia ed Embriologia generale, Università di Pavia, Italy.
A
A
Casasco
A
A
Calligaro
E
E
Casasco
C
C
Marchetti
P
P
Poggi
M
M
Casasco
D000818:Animals; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D001973:Bromodeoxyuridine