An enzyme-linked immunosorbent assay for the determination of chloramphenicol using a monoclonal antibody. Application to residues in swine muscle tissue.
A competitive enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody is described for the determination of chloramphenicol (CAP) residues in swine muscle tissue. The limit of detection of standard solutions is established to be 25 ng ml-1 = 2.0 ng CAP per well). The very high specificity of the monoclonal antibody for CAP is expressed by the insignificant cross-reactivity with other antimicrobial agents and with structurally related compounds. By means of the rapid sample preparation method described earlier for the CAP determination using high-performance liquid chromatography (HPLC), residue levels of CAP in swine muscle tissue above 5 micrograms kg-1 can be easily quantitated. The muscle samples show good recovery percentages at 10-50 micrograms kg-1 spiking levels. The results obtained by the ELISA method were confirmed by HPLC.
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
C
C
van de Water
N
N
Haagsma
P J
PJ
van Kooten
W
W
van Eden
D000818:Animals; D000911:Antibodies, Monoclonal; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D007202:Indicators and Reagents; D009132:Muscles / Q000032:analysis*; D013552:Swine
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D002701:Chloramphenicol