Inhibitory effect of tetrachloro-p-hydroquinone and other metabolites of hexachlorobenzene on hepatic uroporphyrinogen decarboxylase activity with reference to the role of glutathione.
Exposure of rats to HCB caused a dose-dependent depletion of GSH. Chlorophenolic and sulfur-containing metabolites of HCB incubated with GSH-free rat liver cytosolic protein drastically diminished the UROD activity. In addition, HCB also exhibited inhibitory potency. The most effective compounds studied were TCH and its oxidation product, chloranil. Incubation of liver cytosolic protein and of GSH with HCB and its metabolites yielded results that suggested interaction between the compounds and cell constituents--an interaction that may cause inhibition of the hepatic UROD activity in the HCB-exposed organism.
Institute of Toxicology and Pharmacology, Philipps University, Marburg, Federal Republic of Germany.
G
G
Koss
M
M
Losekam
J
J
Seidel
K
K
Steinbach
W
W
Koransky
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002703:Chloranil / Q000494:pharmacology; D002722:Chlorobenzenes / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006581:Hexachlorobenzene / Q000378:metabolism* / Q000494:pharmacology; D006873:Hydroquinones / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D014575:Uroporphyrinogen Decarboxylase / Q000378:metabolism*
D002722:Chlorobenzenes; D006873:Hydroquinones; D002703:Chloranil; D006581:Hexachlorobenzene; C044578:2,3,5,6-tetrachlorohydroquinone; D002262:Carboxy-Lyases; D014575:Uroporphyrinogen Decarboxylase; D005978:Glutathione