[Radioactive labeling of DNA with 3H-thymidine: effect of the internal irradiation on cell growth, chromatin structure and gene activity].
The growth of djungarian hamster fibroblasts 4/21 is inhibited by 3H-thymidine present in a culture medium in concentrations from 18.5 to 740 KBq/ml. As judged from the gradient elution of DNA from isolated nuclei (the nucleoprotein-celite chromatography), DNA fragmentation increases together with the increase in 3H-thymidine concentration and the decrease in the cell growth rate. DNA fragmentation does not activate the family of heat shock genes (hsp70). On the contrary, the hsp70 gene transcription is somewhat inhibited in both heat shock and non-heat shock conditions even at a concentration of 3H-thymidine of as low as 37 KBq/ml. Hence the 3H labelling of radiosensitive cultured cells can lead to some deviations in cellular processes under study.
R P
RP
Alekhina
V L
VL
Moiseev
M M
MM
Zaboĭkin
A V
AV
Likhtenshteĭn
V S
VS
Shapot
D000818:Animals; D002843:Chromatin / Q000528:radiation effects*; D006224:Cricetinae; D004247:DNA / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects; D005796:Genes / Q000528:radiation effects*; D007553:Isotope Labeling; D011831:Radiation Genetics; D014316:Tritium
D002843:Chromatin; D014316:Tritium; D004247:DNA
Radioaktivnoe mechenie DNK 3H-timidinom: vliianie vnutrennego oblucheniia na rost kletok, strukturu khromatina i gennuiu aktivnost'.