Determination of atropine in plasma by a direct radioreceptor assay.
A highly sensitive radioreceptor assay for the anticholinergic atropine was developed and could be applied directly to plasma samples obtained from mini-pigs without any clean-up. Plasma samples were collected during 6 h after atropine was administered intravenously or endobronchially. The endobronchial plasma concentration-time curves were characterized by a very rapid rise of the concentration but with a subsequent much slower decrease than after intravenous administration. This indicates an initially high as well as prolonged uptake from the lungs.
State University of Groningen, Groningen Centre for Drug Research, The Netherlands.
K
K
Ensing
R A
RA
De Zeeuw
U
U
Hörnchen
J
J
Schüttler
H
H
Stoeckel
D000818:Animals; D001285:Atropine / Q000097:blood* / Q000493:pharmacokinetics; D001980:Bronchi; D007275:Injections, Intravenous; D007322:Instillation, Drug; D011869:Radioligand Assay / Q000379:methods*; D013552:Swine
D001285:Atropine