The influence of the lipid composition on the degree of lipid-peroxidation of liposomes.
Liposomes containing dilinoleoyl lecithin (di-18(2)PC) and diarachidonyl lecithin (di-20(4)PC) were peroxidized with Fe++/ascorbate. Lipidperoxidation was measured as generated malondialdehyde, the disappearance of polyunsaturated fatty acids, conjugated diene formation and chemiluminescense. It was found that di-20(4)PC was oxidized rapidly, while in the same time period hardly any oxidation of di-18(2)PC took place. Incorporation of different lipid classes into the di-20(4)PC liposomes influenced the peroxidation for each lipid in a characteristic way. Phosphatidylethanolamine stimulated the formation of fluorescent chromolipids. The possible meaning of these reactions in human pathology is discussed.
Department of Pathology I, Erasmus University Rotterdam, The Netherlands.
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Montfoort
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Bezstarosti
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Groh
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TJ
Metsä-Ketelä
D000447:Aldehydes; D007700:Kinetics; D008054:Lipid Peroxides; D008081:Liposomes; D008163:Luminescent Measurements; D008954:Models, Biological; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines; D013329:Structure-Activity Relationship
D000447:Aldehydes; D008054:Lipid Peroxides; D008081:Liposomes; D010713:Phosphatidylcholines; C044819:1,2-diarachidonoyl-glycero-3-phosphocholine; C002448:1,2-linoleoylphosphatidylcholine