Cloning and expression of the metallo-proteinase inhibitor (S-MPI) gene from Streptomyces nigrescens.
The coding and regulatory regions of a proteinaceous metallo-proteinase inhibitor (S-MPI) gene have been cloned from the genomic DNA of Streptomyces nigrescens using a deoxyinosine-containing synthetic probe designed from the amino acid sequence of the S-MPI protein. The S-MPI gene was located to the DNA region of about 1.2 kilobase pairs, which was verified to be sufficient for the gene expression in S. lividans.
Institute of Applied Microbiology, University of Tokyo, Japan.
K
K
Tanaka
H
H
Saito
K
K
Oda
S
S
Murao
H
H
Takahashi
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D007700:Kinetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D011480:Protease Inhibitors; C056999:metalloproteinase inhibitor, Streptomyces; D008666:Metalloendopeptidases