Non-chromogenic peptide substrates for detection of enzymes by means of capacitance changes at metal electrodes.
A new type of substrate for enzyme detection has been developed. The substrate is non-chromogenic and is used in an assay method based on electrode adsorption. The rate of change in the electric capacitance of the electrode is monitored and taken as a measure of the substrate adsorption. Substrate adsorption is in turn proportional to substrate bulk concentration and thus subject to changes by enzymes. The new substrate introduces a new concept in enzyme detection: as it is non-chromogenic it may contain appropriate amino acids on both sides of the bond subject to enzymatic cleavage.
Arwin
H
H
Department of Physics and Measurement Technology, Linköping Institute of Technology, Sweden.
Agner
E
E
Lundström
I
I
Claeson
G
G
D004563:Electrochemistry; D004566:Electrodes; D004798:Enzymes / Q000032:analysis*; D010455:Peptides
D004798:Enzymes; D010455:Peptides