Preparation of apoE-free rat low density lipoprotein for catabolic studies.
The use of serum from rat fetuses instead of serum from adult rats for preparation of LDL by preparative ultracentrifugation leads to an LDL fraction containing apoB-100 and apoB-95 as the only protein moieties without need for any further purification. The yield of LDL is five times greater compared to the use of adult rat serum. Lipid composition and particle size of LDL from fetal and adult rats are quite similar. The method described allows a simple way for preparation of sufficient amounts of apoE-free LDL for use in metabolic studies.
Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.
B
B
Schlag
L
L
Winkler
D
D
Plonné
U
U
Dürer
R
R
Dargel
D000818:Animals; D001057:Apolipoproteins E / Q000032:analysis*; D005312:Fetal Blood / Q000032:analysis; D008077:Lipoproteins, LDL / Q000032:analysis*; D008722:Methods; D051381:Rats; D011919:Rats, Inbred Strains
D001057:Apolipoproteins E; D008077:Lipoproteins, LDL