Protein supplements, mitogens and antibiotics in lymphoblast transformation and cytogenetic analysis.
The in vitro effects of four protein supplements, foetal calf serum (FCS), gammaglobulin-depleted FCS (FCS-A), human serum albumin (HSA) and autologous serum (AS), and of four antibiotics on lymphocyte blast transformation and on lymphocyte proliferation for cytogenetic studies, were evaluated. 3H-thymidine incorporation was measured in the lymphocytes obtained from sixteen healthy adults and stimulated with phytohaemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), PWM + PHA and PWM + Con A. The most efficient protein supplement was AS, although great variability was observed between subjects. HSA came second, while FCS and FCS-A were the least effective. The presence of immunoglobulins did not modify mitogen-induced lymphocyte responses. In unsupplemented media, peak responses to PHA and Con A occurred at lower mitogen concentrations. Furthermore, the addition of the antibiotics tested did not impede blast transformation whether or not a protein supplement was present. This work also showed that for chromosome analysis, protein supplements are necessary for optimum whole-blood lymphocyte cultures and that HSA can replace FCS.
Department of Anatomy, Faculty of Medicine, Université de Montréal, Québec, Canada.
R
R
Drouin
S
S
Rémy-Prince
C L
CL
Richer
S
S
Montplaisir
D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001769:Blood; D001798:Blood Proteins / Q000494:pharmacology*; D002417:Cattle; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008934:Mitogens / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D012709:Serum Albumin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D008934:Mitogens; D012709:Serum Albumin