Fourier analysis of nuclear and cytoplasmic shape of blood lymphoid cells from healthy donors and chronic lymphocytic leukemia patients.
The folding rates of the contours of nuclei and entire lymphoid cells were analyzed by Fourier analysis of the shapes. Smears of peripheral blood from healthy subjects and from patients with chronic lymphocytic leukemia (CLL: type B, stage zero) were routinely prepared and stained. The shapes of lymphoid cells from CLL patients revealed a higher folding rate (from fifth to tenth harmonics) than did those of lymphocytes from healthy subjects. Accordingly, the roughness coefficient (describing the folding rate of the surface) for CLL cells was 0.036, as compared to 0.028 for the cells of healthy subjects. The shapes of nuclei of CLL lymphoid cells also had a higher folding rate than did those of lymphocytes from healthy subjects, but a significant difference was found only for the highest harmonic calculated (the tenth harmonic); the respective roughness coefficients for nuclei were 0.037 and 0.033.
Department of Histology and Embryology, School of Medicine, Central Clinical Hospital, Warsaw, Poland.
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D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D005583:Fourier Analysis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology*; D008214:Lymphocytes / Q000648:ultrastructure*