Time, a quality-control parameter in flow cytometry.
Time has been used as a quality-control parameter in our flow cytometer. This parameter was automatically incorporated into the list-mode data base by hooking the computer clock directly into the acquisition logic. Quality control can then be checked by "replaying" fluorescence or light scatter data vs. time and any instrumental drift can be observed and degraded data can be excised. The technique is illustrated with a number of DNA data sets from a tissue culture cell line in which artificial perturbations were introduced during data acquisition to simulate potential causes of instrumental drift.
MRC Clinical Oncology Unit, Medical School, Cambridge, England.
J V
JV
Watson
D002460:Cell Line; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000592:standards*; D006801:Humans; D008027:Light; D011786:Quality Control; D012542:Scattering, Radiation; D013997:Time Factors
D004247:DNA