Implantation of fibroblasts into vitrectomized eyes. Dose-response relationship and the putative inhibitory effect of sodium hyaluronate.
To determine whether or not sodium hyaluronate (NaHA) has any inhibitory effect on cellular proliferation in the vitreous space, we implanted 3 X 10(5) or 1 X 10(6) homologous fibroblasts into the vitreous cavity of 21 vitrectomized albino rabbits. Sixteen eyes received 0.7-0.8 ml of a 1% solution of NaHA intravitreally and 18 eyes got BSS only. Ophthalmoscopy and histological examination showed that 5 of 10 BSS-injected and 2 of 8 NaHA-injected eyes in the group receiving 3 X 10(5) fibroblasts developed retinal detachment (RD) after 4-8 weeks. All BSS- and NaHA-injected eyes implanted with 1 X 10(6) fibroblasts developed RD. The results indicate that NaHA has an unsatisfactory inhibitory effect on fibrovascular growth in response to moderate and large inocula of fibroblasts.
Department of Ophthalmology, Karolinska Institute and Hospital, Stockholm, Sweden.
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Algvere
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Landau
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000473:pathology / Q000637:transplantation*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007267:Injections; D011817:Rabbits; D012163:Retinal Detachment / Q000209:etiology; D012867:Skin; D012965:Sodium Chloride / Q000494:pharmacology; D014821:Vitrectomy; D014822:Vitreous Body / Q000473:pathology*
D012965:Sodium Chloride; D006820:Hyaluronic Acid