Spontaneous neoplastic evolution of Swiss mouse embryo cells (PMS-14) in culture.
Some biological properties related to transformation have been studied to characterize a spontaneously transformed mouse embryonic fibroblast cell line, PMS-14. Low population doubling time with moderately high plating efficiency and saturation density were noted. These cells showed reduced adhesion to substratum, enhanced agglutinability by concanavalin A, anchorage independent growth in soft agar and tumorigenicity in mice.
Department of Tissue Culture, Chittaranjan National Cancer Research Centre, Calcutta, India.
P
P
Mukherjee
S K
SK
Das
D000818:Animals; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts; D051379:Mice