A reversed-phase high-performance liquid chromatographic method for characterization of biosynthetic human growth hormone.
An isocratic reversed-phase high-performance liquid chromatographic method for the determination of human growth hormone (HGH) purity is described. This method offers superior resolution of HGH-related substances (e.g., sulfoxide and desamido derivatives) from unmodified HGH when compared to a number of alternative chromatographic and electrophoretic techniques.
Bioanalytical Development Department, Eli Lilly and Company, Indianapolis, Indiana 46285.
R M
RM
Riggin
G K
GK
Dorulla
D J
DJ
Miner
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D013006:Growth Hormone / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans
D013006:Growth Hormone