Carbohydrate moieties of peanut agglutinin receptors isolated from human gastric cancer cells.
Receptors for peanut agglutinin (PNA) were isolated from Kato III human gastric cancer cells by affinity chromatography on PNA agarose, and were labeled by the galactose oxidase-NaB3H4 method. Alkaline NaBH4 treatment of the labeled receptors released two small oligosaccharide alcohols, which were identified as Gal beta 1----3GalNAc-ol and Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAc-ol. Higher oligosaccharides and glycopeptides of both N- and O-linked type were also detected, but they did not appear to bear PNA binding sites. The presence of oligo-N-acetyllactosamine units in the N-linked type sugars was indicated by endo-beta-galactosidase digestion.
Department of Biochemistry, Faculty of Medicine, Kagoshima University.
N
N
Shimoda
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H
Muramatsu
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T
Osawa
T
T
Yamada
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M
Adachi
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T
Muramatsu
D002241:Carbohydrates / Q000032:analysis* / Q000302:isolation & purification; D002854:Chromatography, Paper; D005959:Glucosidases; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D011975:Receptors, Mitogen / Q000032:analysis*; D013274:Stomach Neoplasms / Q000032:analysis*
D002241:Carbohydrates; D009844:Oligosaccharides; D011975:Receptors, Mitogen; C038223:peanut agglutinin receptor; D005959:Glucosidases