Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals.
We have introduced the firefly luciferase gene of Photinus pyralis into the vaccinia virus genome. This gene is expressed in a coordinate fashion during virus infection. Luminescence produced by the action of luciferase [Photinus-luciferin:oxygen 4-oxidoreductase(decarboxylating, ATP-hydrolyzing), EC 1.13.12.7] was easily detectable in infected cells in culture as well as in cells of tissues of infected mice. The limits of detection were about one infected cell in a background of a million noninfected cells. The luciferase assay was about 1000-fold more sensitive than that of beta-galactosidase. Our findings show that the luciferase assay can be conveniently used to follow viral gene expression and virus dissemination both in cell cultures and in tissues of infected animals.
Department of Biochemistry, State University of New York Health Science Center at Brooklyn 11203.
J F
JF
Rodriguez
D
D
Rodriguez
J R
JR
Rodriguez
E B
EB
McGowan
M
M
Esteban
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D008156:Luciferases / Q000235:genetics*; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D014616:Vaccinia virus / Q000235:genetics*; D014779:Virus Replication
D008156:Luciferases