Semi-automated enzyme-linked immunosorbent assay (ELISA) for the quantification of apolipoprotein B using monoclonal antibodies.
A semi-automated competitive, double-antibody, solid-phase enzyme-linked immunosorbent assay for apolipoprotein B (Apo B) has been developed which utilizes microtiter plates with commercially available monoclonal antibodies and alkaline phosphatase-conjugated second antibody. The working range of the assay is 20-200 ng. The concentration of plasma Apo B was 0.88 +/- 0.20 g/l (n = 40) for a random sample of normal adults. The correlation coefficient for this assay, compared to a radial immunodiffusion assay, was 0.95 (slope = 1.13, intercept = -15). The quantification of the samples was not influenced by freezing and thawing, storage at -20 degrees C for up to 9 mth, or the lipoprotein particle on which the Apo B was present. The method is suitable for measurement of apolipoprotein B in either normal or pathological plasma, lipoprotein density classes, and is sensitive enough to quantify Apo B in cell biological and molecular biological investigations.
Bojanovski
M
M
Medizinische Hochschule Hannover, Department of Biochemistry, FRG.
Gregg
R E
RE
Wilson
D M
DM
Brewer
H B
HB
D000433:1-Propanol; D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B / Q000097:blood*; D001322:Autoanalysis / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B; D000433:1-Propanol