Neuropeptide Y gene expression in PC12 cells and its regulation by nerve growth factor: a model for developmental regulation.
Neuropeptide Y (NPY) is a 36 amino acid neuronal peptide which has previously been described within a subset of sympathetic neurons. The presence of the messenger RNA that encodes NPY (NPY mRNA) has been studied in the PC12 cell line, as this is commonly used as a model of neuronal differentiation. Low levels of the NPY mRNA were identified in resting naive cells. Levels were induced five-fold by the addition of nerve growth factor to the medium at a dose of 30 ng/ml. Lower doses of nerve growth factor had no effect on NPY mRNA. The effect was rapid in onset being apparent within 12 h and near maximal at 24 h. A smaller two-fold increase in NPY mRNA was observed in cells treated with epidermal growth factor (3 ng/ml) over the same time course but no effect was observed with fibroblast growth factor (3 ng/ml), bradykinin (10(-6) M) or dexamethasone (10(-6) M). These results indicate that NPY gene expression is regulated in PC12 cells at the level of NPY mRNA.
Department of Neurology, Massachusetts General Hospital, Boston 02114.
J M
JM
Allen
J B
JB
Martin
G
G
Heinrich
D000818:Animals; D002454:Cell Differentiation; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation; D005796:Genes; D009414:Nerve Growth Factors / Q000494:pharmacology / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009478:Neuropeptide Y / Q000235:genetics*; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D009478:Neuropeptide Y; D003907:Dexamethasone