Optimization of the reversed-phase high-performance liquid chromatographic separation of synthetic estrogenic and progestogenic steroids using the multi-criteria decision making method.
The optimization of the reversed-phase high-performance liquid chromatographic separation of a mixture of ethynylestradiol, desogestrel and three related compounds is described. A procedure is used that allows the prediction of the capacity factors of each individual synthetic steroid, depending on the mobile phase composition. Therefore, complete chromatograms can be predicted and evaluated with the multi-criteria decision making method.
Smilde
A K
AK
Department of Analytical Chemistry and Toxicology, Faculty of Pharmacy, Groningen, The Netherlands.
Bruins
C H
CH
Doornbos
D A
DA
Vink
J
J
D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000302:isolation & purification*; D011372:Progestins / Q000302:isolation & purification*
D004967:Estrogens; D011372:Progestins