[Effector role of fatty acids in the transformation of methemoglobin into hemichrome].
The interaction of fatty acids (FA) with methemoglobin (met-Hb) was investigated. It was found that under the action of FA met-Hb is rapidly converted into a low-spin form (the so-called hemichrome). This process is reversible as proved by the addition of bovine serum albumin into the system. Using differential spectrophotometry, the efficiency of FA action on met-Hb depending on concentration and nature of FA was estimated. Using the circular dichroism (CD) method, it was demonstrated that under the action of FA the concentration of R-conformation in the met-Hb molecule is increased. The stoichiometry of the hemichrome complex with FA at various FA concentrations was assessed. A comparative analysis of the effect of various detergents with known structures on met-Hb was carried out. The mechanism of met-Hb conversion into hemichrome is discussed.
A A
AA
Akhrem
G M
GM
Andreiuk
M A
MA
Kisel'
V Z
VZ
Kurbako
P A
PA
Kiselev
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D005227:Fatty Acids / Q000378:metabolism*; D006420:Hemeproteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008706:Methemoglobin / Q000378:metabolism*; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
D005227:Fatty Acids; D006420:Hemeproteins; C001332:hemichrome; D008706:Methemoglobin
Effektornaia rol' zhirnykh kislot v protsesse perekhoda metgemoglobina v gemikhrom.