The growth of ordered two-dimensional sheets of ribosomal particles from salt-alcohol mixtures.
A procedure for the in vitro growth of well-ordered two-dimensional sheets from ribosomal particles using salts and salt-alcohol mixtures has been developed. Employing this procedure, ordered two-dimensional sheets of the wild type as well as of mutated 50 S ribosomal subunits from Bacillus stearothermophilus can readily be obtained. These sheets, stained with uranyl acetate or gold-thioglucose, are suitable for three-dimensional image reconstruction. They consist of relatively small unit cells with dimensions of 160 +/- 15 and 365 +/- 20 A. Diffraction patterns of electron micrographs of these sheets contain features to 25 A resolution.
Arad
T
T
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.
Piefke
J
J
Gewitz
H S
HS
Romberg
B
B
Glotz
C
C
Müssig
J
J
Yonath
A
A
Wittmann
H G
HG
D003460:Crystallization; D001411:Geobacillus stearothermophilus; D012270:Ribosomes / Q000648:ultrastructure*