[Changes in erythrocyte deformability in blood preservation].
The deformability changes of erythrocytes during storage were characterized by a filtration procedure for blood units mainly used for erythrocyte substitution in the GDR. Following order of maintaining deformability properties were measured: 1. At-196 degrees C cryopreserved erythrocytes; 2. whole blood units; 3. at-28 degrees C cryo-preserved erythrocytes (in clinical examination); 4. buffy-coat free erythrocyte concentrates resuspended with CDS-AG medium. The drastic deformability loss of CDS-AG resuspended erythrocytes (75% of all erythrocyte transfusions!) is caused by an increase of the internal viscosity due to a practically irreversible shrinkage of erythrocytes as an effect of low ionic strength. In contrast to former data a reinvestigation of in vivo survival resulted in values 20% below the limit of 70% 24 h p.i. In comparison to this the abroad used CPDA-1 whole blood units and SAGM resuspensions showed only small deformability changes during storage. By a change of composition of the GDR-resuspension medium the drastic deformability loss is avoided. This new resuspension medium is concerning the deformability loss comparable to the SAGM medium. A comparison of deformability changes during storage in plastic bags and glass bottles as well as in media with various hemolysis reducing agents did not show any differences.
Institut für Transfusiologie und Transplantologie, Humboldt-Universität Berlin.
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D001793:Blood Preservation / Q000379:methods*; D004905:Erythrocyte Aging; D004907:Erythrocyte Deformability; D005615:Freezing; D005859:Germany, East; D006400:Hematocrit; D006452:Hemoglobinometry; D006801:Humans
Veränderungen der Erythrozytendeformierbarkeit während der Blutkonservierung.