Determination of methylprednisolone metabolites in human urine by gas chromatography-mass spectrometry.
Methylprednisolone and its metabolites were studied as their methoxyamine-trimethylsilyl derivatives by means of capillary gas chromatography-mass spectrometry. The expected unchanged drug and 11-keto and 20-hydroxy metabolites were found in human urine. The typical metabolites are 6,7-dehydro analogues of the above-mentioned compounds. Characteristic gas chromatographic profiles of urine steroids were obtained. Retention indices and m/z values are presented for methylprednisolone and its main metabolites.
Rodchenkov
G M
GM
Anti-Doping Centre, Central Institute of Sports Medicine, Moscow, U.S.S.R.
Uralets
V P
VP
Semenov
V A
VA
D001711:Biotransformation; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008775:Methylprednisolone / Q000652:urine*
D008775:Methylprednisolone