Reconstitution and crystallisation experiments with isolated split proteins from Bacillus stearothermophilus ribosomes.
Six proteins (B-L1, B-L6, B-L10, B-L11, B-L12 and B-L16) were removed from 50S ribosomal subunits of Bacillus stearothermophilus by treatment with ethanol and ammonium chloride. The proteins were isolated in a pure form, and one of them (B-L6) was crystallized. Five of the six proteins (in various combinations) were added back to the core particles, resulting in 50S subunits lacking one protein. The biological activities of these ribosomal particles as determined in the poly(U)-system varied over a wide range, depending on the protein which was omitted. The particles lacking one protein provide useful tools for heavy-atom derivation necessary for our crystallographic studies on the 50S subunits of Bacillus stearothermophilus.
Max-Planck-Institut für Molekulare Genetik, Berlin.
H S
HS
Gewitz
C
C
Glotz
P
P
Goischke
B
B
Romberg
J
J
Müssig
A
A
Yonath
H G
HG
Wittmann
D001426:Bacterial Proteins / Q000145:classification / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D001411:Geobacillus stearothermophilus / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D012270:Ribosomes / Q000032:analysis*; D009626:Terminology as Topic
D001426:Bacterial Proteins