[Sialic acid containing compounds in the hemofiltrate of patients with chronic renal insufficiency. II. Isolation and structure determination of sialoglycopeptides].
Four sialyl glycopeptides have been isolated from the hemofiltrate of a patient with end stage renal disease using reverse osmosis, gel filtration and ion-exchange chromatography. Structural studies including one- and two-dimensional 1H-NMR spectroscopy, FAB mass spectrometry and enzymatic degradation indicated the following structures: (Formula: see text). While the disialyl glycopeptide has been previously characterized from normal and pregnancy urine, the three other sialyl glycopeptides could be isolated for the first time from biological material. The origin of these compounds and their possible clinical relevance is subject to further investigations.
Organisch-Chemisches Institut Universität Heidelberg.
G
G
Weisshaar
W
W
Baumann
H
H
Friebolin
H
H
Brunner
H
H
Mann
H G
HG
Sieberth
H J
HJ
Opferkuch
D005260:Female; D006440:Hemofiltration; D006801:Humans; D006868:Hydrolysis; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D012794:Sialic Acids / Q000097:blood*; D012795:Sialoglycoproteins / Q000097:blood / Q000302:isolation & purification*
D012794:Sialic Acids; D012795:Sialoglycoproteins
Sialinsäurehaltige Verbindungen im Hämofiltrat von Patienten mit chronischer Niereninsuffizienz, II. Isolierung und Strukturaufklärung von Sialoglycopeptiden.