[Effects of systemic administration of strychnine, L-allylglycine, bicuculline and picrotoxin on the transsynaptic neural destruction in the medullary dorsal horn following transection of the rat inferior alveolar nerve].
Adult rats underwent unilateral transection of the inferior alveolar nerve and subsequent intraperitoneal injection of strychnine (1 mg/kg, 3-23 days) at various posttransectional intervals. When they were sacrificed at 18-30 days posttransectionally, many pyknotic neuronal cell bodies were observed in plastic embedded toluidine blue-stained 1 micron-thick sections of the medullary dorsal horn. They were mostly found in the dorsal part of the dorsal horn ipsilateral to the neurotomy and were more abundant in laminae I/II than in laminae III/IV. Similar pyknotic neurons were found after 1 or 2 days of L-allylglycine administration (55.7 mg/kg/day) at a posttransectional interval of 20 days. Unlike those observed after strychnine treatment, the pyknotic neurons after L-allylglycine treatment were evenly distributed throughout laminae I-IV of the dorsal half of the medullary dorsal horn. Twenty-three days of bicuculline (2 mg/kg/day) or picrotoxin (0.5 mg/kg/day) treatment at an interval of 7 days did not yield pyknotic neurons. The results are discussed in the light of intrinsic synaptic circuitry of the dorsal horn.
2nd Department of Oral Anatomy, Osaka University, Faculty of Dentistry.
M
M
Takemura
T
T
Sugimoto
A
A
Sakai
D000501:Allylglycine / Q000494:pharmacology*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology*; D005998:Glycine / Q000031:analogs & derivatives*; D008297:Male; D008340:Mandibular Nerve / Q000502:physiology*; D008526:Medulla Oblongata / Q000473:pathology*; D009410:Nerve Degeneration / Q000187:drug effects*; D010852:Picrotoxin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013331:Strychnine / Q000494:pharmacology*
D000501:Allylglycine; D001640:Bicuculline; D005998:Glycine; D010852:Picrotoxin; D013331:Strychnine