Carrier erythrocytes from cattle can be prepared from cells stored in a hypotonic, porous, dialyzed state.
Hypotonic dialysis of bovine erythrocytes to 160-180 mosmol/kg produces cells with metastable pores. Cells stored for 3 weeks in a hypotonic dialyzed state can be used to prepare carrier erythrocytes. Pores generated by nonlytic hypotonic conditions are initially greater than 40 A in radius but shrink to less than 20 A with storage beyond 1 week. A metastable pore allows for hemoglobin isolation from hypotonically dialyzed cells.
Veterinary Toxicology and Entomology Research Laboratory, U.S. Department of Agriculture, College Station, Texas 77841.
J R
JR
DeLoach
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K
Andrews
D000818:Animals; D001793:Blood Preservation; D002250:Carbon Radioisotopes; D002417:Cattle; D003956:Dialysis; D004912:Erythrocytes / Q000648:ultrastructure; D007038:Hypotonic Solutions; D007444:Inulin / Q000097:blood; D008855:Microscopy, Electron, Scanning; D013395:Sucrose / Q000097:blood; D014316:Tritium
D002250:Carbon Radioisotopes; D007038:Hypotonic Solutions; D014316:Tritium; D013395:Sucrose; D007444:Inulin