Isolation and characterization of basement membrane from frog skeletal muscle.
Isolation of basement membrane from frog skeletal muscle has been described. The membrane preparation contained 35 micrograms hexoses, 1.72 micrograms sialic acid, 6.8 micrograms phospholipids, 0.21 micrograms cholesterol/mg protein. Na + K-ATPase and 5'-nucleotidase could not be detected in the membrane preparation. Glycine accounted for about 20% of the total amino acids. On SDS-PAGE, the membrane resolved into 20-22 polypeptide bands.
Industrial Toxicology Research Centre, Lucknow, India.
N
N
Ali
R K
RK
Upreti
M
M
Baijal
A M
AM
Kidwai
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011898:Ranidae; D012508:Sarcolemma / Q000032:analysis*
D000596:Amino Acids; D002241:Carbohydrates; D010743:Phospholipids; D011506:Proteins; D002784:Cholesterol; D006909:Hydroxyproline