Does calmodulin participate as an intermediate in the process of glycogenolysis in rat submandibular gland?
A role of calmodulin in the process of the glycogenolysis induced by adrenaline was investigated in rat submandibular gland. When the slice was incubated in the Ca2+-free medium, the adrenaline-induced glycogenolysis was significantly inhibited. Moreover, the inhibition of glycogenolysis induced in the Ca2+-free medium was further enhanced by addition of 2 mM EGTA. Chlorpromazine, trifluoperazine and W-7, calmodulin antagonists, had no effect on the glycogenolysis induced by adrenaline. These results suggest that the adrenaline-induced glycogenolysis is dependent on the presence of Ca2+ and that calmodulin does not participate in the process of glycogenolysis in rat submandibular gland.
Department of Pharmacology, Tokyo College of Pharmacy, Japan.
T
T
Komabayashi
T
T
Izawa
K
K
Suda
S
S
Shinoda
M
M
Tsuboi
D000818:Animals; D002147:Calmodulin / Q000494:pharmacology*; D002746:Chlorpromazine / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology
D002147:Calmodulin; D013449:Sulfonamides; D014268:Trifluoperazine; C017967:W 7; D006003:Glycogen; D002746:Chlorpromazine