Angiotensin II immunoreactivity in push-pull perfusates from the paraventricular nucleus.
Push-pull perfusion of the hypothalamic paraventricular nucleus in sodium pentobarbital anesthetized Sprague-Dawley rats indicates the release of angiotensin II-immunoreactive material in this area. Attempts to demonstrate a neuronal origin of this material by chemical depolarization with perfusate containing either 40 or 120 mM K+ were unsuccessful. However, this material does appear to be of central origin since intravenous infusion of arginine-vasopressin, a similar sized peptide, did not result in the appearance of increased levels of this substrate in the perfusate, indicating that the integrity of the blood-brain barrier was not compromised by the perfusion.
Doris
P A
PA
Department of Cell Biology and Anatomy, Texas Tech University Health Sciences Center, School of Medicine, Lubbock 79430.
D000804:Angiotensin II / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis / Q000097:blood; D010286:Paraventricular Hypothalamic Nucleus / Q000032:analysis*; D010477:Perfusion; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D000804:Angiotensin II; D001127:Arginine Vasopressin