Affinity purification of human brain tissue factor utilizing factor VII bound to immobilized anti-factor VII.
An efficient procedure for affinity purification of human tissue factor apoprotein that requires binding of only microgram quantities of human factor VII to anti-factor VII agarose is described. Factor VII was added to a 2% Triton X-100 extract of acetone brain powder in the presence of calcium. The resultant factor VII/tissue factor/calcium complex was bound to anti-factor VII-agarose, and the bound tissue factor was then eluted with EDTA. The eluate was passed through anti-goat IgG-agarose to remove contaminating goat IgG that leaks from the anti-factor VII column. Yield (units of activity) was 27%; specific activity was 2400 U/mg, which corresponds to that reported by others. The purified apoprotein migrated as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent molecular weight of 47,000. Immunostaining with a goat anti-tissue factor IgG raised against the purified material yielded a major band of the same apparent molecular weight. Factor VII remains bound to the column and, therefore, for subsequent use preincubation of tissue factor with factor VII and calcium is not required. Thus, the present purification procedure markedly reduces the amount of factor VII needed as affinity ligand to purify tissue factor apoprotein.
Department of Medicine, University of California at San Diego, La Jolla 92093.
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Rao
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Rapaport
D001923:Brain Chemistry; D002846:Chromatography, Affinity / Q000379:methods; D005167:Factor VII; D006801:Humans; D007457:Iodine Radioisotopes; D013925:Thromboplastin / Q000302:isolation & purification* / Q000378:metabolism
D007457:Iodine Radioisotopes; D005167:Factor VII; D013925:Thromboplastin