Determination of preservatives in cosmetic products. I. Thin-layer chromatographic procedure for the identification of preservatives in cosmetic products.
A thin-layer chromatographic procedure is presented for the separation and identification of preservatives that are listed in the current EEC Council Directive on cosmetic products or have been permitted in the past. The method consists of an extraction of acidified cosmetics with methanol, separation of the extracts by thin-layer chromatography on aluminium oxide and silica gel-coated plates using one developing solvent, and visualization of the preservatives on the plates using short-wavelength UV light and six detection reagents. The retention behaviour and the detectability of 88 preservatives were investigated, of which 74 were characterized by this method. The preservatives in fourteen commercial cosmetic products were tentatively identified by the procedure described. In general this method will permit the routine detection of preservatives in cosmetics in an approximate concentration of 0.1% (w/w).
De Kruijf
N
N
TNO-CIVO Food Analysis Institute, Department of Toxicological Analysis, Zeist, The Netherlands.
Rijk
M A
MA
Pranoto-Soetardhi
L A
LA
Schouten
A
A
D002855:Chromatography, Thin Layer; D003358:Cosmetics / Q000032:analysis*; D007202:Indicators and Reagents; D010592:Pharmaceutic Aids / Q000032:analysis*; D011310:Preservatives, Pharmaceutical / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D003358:Cosmetics; D007202:Indicators and Reagents; D010592:Pharmaceutic Aids; D011310:Preservatives, Pharmaceutical