Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells.
TK mRNA levels were determined in mouse L cells transformed with intron deletion mutations of the chicken TK gene. Whether normalized per cell, per integrated gene, or per internal control signal, intron deletion did not diminish the efficiency of TK mRNA formation in transformed L cells. The results demonstrated that introns are not required for efficient biogenesis of cellular mRNA in transformed mouse L cells.
Gross
M K
MK
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Kainz
M S
MS
Merrill
G F
GF
D000818:Animals; D002645:Chickens; D007438:Introns; D007739:L Cells; D051379:Mice; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis*; D012260:Ribonucleases; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D012333:RNA, Messenger; D013937:Thymidine Kinase; D012260:Ribonucleases