Hemolytic activity of and lethal toxin production by environmental strains of Vibrio parahaemolyticus.
Repeated subculturing of Kanagawa-negative strains of Vibrio parahaemolyticus on Wagatsuma agar induced the production of a hemolysin which was not the thermostable direct hemolysin. Crude hemolysin exhibited a 30 to 40% lethal toxicity in mice after intraperitoneal injection. A 21-kilodalton protein band was observed with all the environmental isolates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results suggested that a certain percentage of environmental strains of V. parahaemolyticus is responsible for pathogenesis.
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
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D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D006460:Hemolysin Proteins / Q000032:analysis / Q000096:biosynthesis*; D006461:Hemolysis; D051379:Mice; D014736:Vibrio parahaemolyticus / Q000378:metabolism*
D001427:Bacterial Toxins; D006460:Hemolysin Proteins