The use of a panel of monoclonal antibodies in the evaluation of cytologic specimens from the central nervous system.
A panel of monoclonal antibodies was tested immunohistochemically to determine the utility of such reagents in distinguishing among metastatic carcinoma, lymphoma, leukemia and primary brain tumors. The monoclonal antibodies used were: (1) a cocktail comprised of three anti-glial fibrillary acidic protein antibodies (alpha-GFAP); (2) UJ13A, a panneuroectodermal antibody; (3) B72.3, which recognizes a carcinoma-distinctive tumor-associated glycoprotein complex; and (4) 2D1, a pan-leukocyte antibody. Fifty-three specimens (21 cerebrospinal fluids, 1 ventricular fluid, 2 brain cyst fluids, 12 needle washings, 15 imprints, 1 subdural fluid and 1 post-shunt fluid) were obtained from 21 gliomas, 2 meningiomas, 1 pineoblastoma, 11 metastatic tumors, 3 lymphomas, 1 leukemia and 14 cases without tumor. alpha-GFAP stained all 21 gliomas and 5 of 5 cases containing reactive brain fragments. UJ13A had a reactivity pattern similar to that of alpha-GFAP, but also stained the meningiomas, pineoblastoma, oat-cell carcinoma and embryonal rhabdomyosarcoma. B72.3 stained all adenocarcinomas and the large-cell carcinoma. 2D1 stained lymphoma and leukemia, all inflammatory cells and 4 of 12 glioblastomas. Although no single antibody was diagnostic of a specific tumor type, this panel accurately differentiated among most primary brain tumors, metastases, leukemias and lymphomas.
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
W W
WW
Vick
C J
CJ
Wikstrand
D E
DE
Bullard
J
J
Kemshead
H B
HB
Coakham
J
J
Schlom
W W
WW
Johnston
D D
DD
Bigner
S H
SH
Bigner
D000328:Adult; D000911:Antibodies, Monoclonal; D001921:Brain / Q000166:cytology / Q000473:pathology; D001932:Brain Neoplasms / Q000473:pathology; D002490:Central Nervous System / Q000166:cytology / Q000473:pathology*; D002555:Cerebrospinal Fluid / Q000166:cytology; D002675:Child, Preschool; D004660:Encephalitis / Q000473:pathology; D005260:Female; D006801:Humans; D007938:Leukemia / Q000473:pathology; D008223:Lymphoma / Q000473:pathology; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000473:pathology
D000911:Antibodies, Monoclonal