[Ultrastructural characteristics of epidermocytes cultured on media with a standard concentration of calcium ions and on hypocalcic media].
A study was made of the ultrastructural changes of epidermocytes cultured on hypocalcic media (Ca2+ content below 0.08 mM) in comparison with epidermocytes, cultured on media with standard Ca2+ content (1-2 mM). A detailed description of methods for epidermocyte cultivation and data of electron microscopic cell research is presented. It has been established that the use of hypocalcic nutrient medium accelerates the process of cultured epithelial sheet formation for grafting in skin defects. However, before removing a sheet from the cultural vessel bottom the nutrition media should be supplemented with Ca2+ to strengthen intercellular coupling of epidermocytes. Thus, Ca2+ addition to the medium can regulate the generation of desmosomes and the formation of the monolayer of stratified sheet of the cultured epithelium.
Tumanov
V P
VP
Gasanov
S
S
Serov
G G
GG
Ustinov
T S
TS
Chervonskaia
N V
NV
D002118:Calcium / Q000008:administration & dosage / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism*; D003896:Desmosomes / Q000648:ultrastructure; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D013997:Time Factors
D003470:Culture Media; D002118:Calcium
Ul'trastrukturnaia kharakteristika épidermotsitov, kul'tivirovannykh v sredakh so standartnoĭ kontsentratsieĭ ionov kal'tsiia i v gipokal'tsievykh sredakh.