Analysis of structural changes in the stria vascularis following chronic gentamicin treatment.
Changes in the stria vascularis following chronic gentamicin treatment have been examined using quantitative methods. Albino guinea pigs were given gentamicin at 100 mg.kg-1.day-1 subcutaneously for 10 days. Comparisons were made of strial tissue from the treated animals sacrificed either 1 h or 4 weeks following the last injection with that from saline-injected controls. Strial width (spiral prominence to Reissner's membrane) and marginal cell (MC) number across the stria were determined from scanning electron micrographs. Strial thickness (endolymphatic surface to spiral ligament) and the volume fractions of the strial components (MCs, intermediate cells (ICs), basal cells (BCs) and capillaries) were derived from thin sections. Qualitative changes to both MCs and ICs were apparent 1 h after the last injection. At four weeks post-treatment, there was a small, but statistically significant, decrease in the number of marginal cells and a highly significant decrease in strial thickness. This was almost entirely due to a highly significant decrease in the volume fraction of MCs (i.e. shrinkage). The volume fraction of ICs was increased but this could be accounted for by MC shrinkage; after allowing for the reduction in strial thickness, the volume occupied by ICs was unchanged. Thus, following chronic gentamicin treatment, the stria is affected but significant progressive and permanent structural effects are confined to the marginal cells.
EM Unit, Institute of Laryngology and Otology, London, U.K.
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Forge
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Wright
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Davies
D000818:Animals; D002452:Cell Count / Q000187:drug effects; D003051:Cochlea / Q000648:ultrastructure*; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage* / Q000494:pharmacology; D006168:Guinea Pigs; D007279:Injections, Subcutaneous; D008297:Male; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000187:drug effects / Q000648:ultrastructure; D013316:Stria Vascularis / Q000187:drug effects / Q000648:ultrastructure*; D013997:Time Factors
D005839:Gentamicins