Effects of 1,2-dibromoethane on isolated hepatocytes: functional alterations and induction of lipid peroxidation.
1. Isolated rat hepatocytes were exposed to the fumigant 1,2-dibromoethane (DBE) and cytotoxicity was evaluated by studying parameters of cellular function and lipid peroxidation. 2. DBE caused plasma membrane damage, as determined by leakage of lactate dehydrogenase, and was more severe in shaken suspensions than stationary suspensions, suggesting that cells were more fragile after DBE exposure. 3. DBE decreased hepatocyte glycogen content and stimulated albumin synthesis in hepatocyte suspensions. 4. Lipid peroxidation resulted from DBE exposure and was greater in cells isolated from phenobarbital-pretreated rats. Shaking the suspensions enhanced lipid peroxidation. Ethane production did not parallel formation of thiobarbituric acid reactants, suggesting that these parameters of lipid peroxidation reflect different mechanisms of molecular interaction of DBE.
Department of Veterinary Pharmacology, Pharmacy and Toxicology, University of Utrecht, The Netherlands.
A A
AA
van Iersel
I
I
van Holsteijn
B J
BJ
Blaauboer
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D006842:Hydrocarbons, Brominated / Q000494:pharmacology*; D007700:Kinetics; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000096:biosynthesis
D002273:Carcinogens; D006842:Hydrocarbons, Brominated; D008054:Lipid Peroxides; D008112:Liver Glycogen; D012709:Serum Albumin; C027947:methylene bromide