Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity.
The leaves of the persimmon Diospyros kaki, have been traditionally used for treatment of hypertensive diseases in Japan. We have studied the inhibitory effects of four flavonoids isolated from the leaves of the persimmon on angiotensin-converting enzyme activity. The four flavonoids astragalin [1], kaempferol-3-O-(2"-O-galloyl)-glucoside [2], isoquercitrin [3], and quercetin-3-O-(2"-O-galloyl)-glucoside [4] inhibited the angiotensin-converting enzyme activity in a dose-dependent fashion. Compounds 1-4 produced 67%, 53%, 33%, and 48% inhibition at a concentration of 300 micrograms/ml, respectively. The 50% inhibitory concentrations (IC50) of 1 and 2 for the angiotensin-converting enzyme were 180 micrograms/ml and 280 micrograms/ml, respectively. On the other hand, 2 and 4 were shown to have tannin activities, but 1 and 3 had no tannin activities. These results suggest that there is no relationship between the inhibition for angiotensin converting enzyme activity and the tannin activity for the four flavonoids.
School of Medicine, Ehime University, Japan.
K
K
Kameda
T
T
Takaku
H
H
Okuda
Y
Y
Kimura
T
T
Okuda
T
T
Hatano
I
I
Agata
S
S
Arichi
D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D008168:Lung / Q000201:enzymology; D010944:Plants / Q000032:analysis*; D051381:Rats; D013634:Tannins / Q000032:analysis / Q000494:pharmacology
D000806:Angiotensin-Converting Enzyme Inhibitors; D005419:Flavonoids; D013634:Tannins