[Comparative study of carbohydrate chains of H1 hemagglutinins of influenza viruses A/Kiev/59/79 and X/Leningrad/54/1 using oligosaccharide maps].
For comparative study of carbohydrate chains of N-glycoproteins, method of "oligosaccharide maps" has been developed. It consists in fractionation of reduced oligosaccharide fragments by gel-chromatography and HPLC on reverse phase and amino columns. Using two HPLC retention time values for each oligosaccharide, two-dimensional maps for both variants of H1 hemagglutinin were constructed. The monosaccharide composition of the majority of oligosaccharides isolated was also elucidated. The carbohydrate chain's patterns for the H1 hemagglutinin variants were found to be similar but to differ considerably from those for H3 hemagglutinin. The data obtained show that the glycosylation pattern depends on virus strain, i.e. on the structure of the polypeptide chain of hemagglutinin.
Arbatskiĭ
N P
NP
Zheltova
A O
AO
Senchenkova
S N
SN
Iurtov
D V
DV
Derevitskaia
V A
VA
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006389:Hemagglutinins, Viral / Q000032:analysis*; D009980:Influenza A virus / Q000032:analysis*; D009005:Monosaccharides / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*
D006389:Hemagglutinins, Viral; D009005:Monosaccharides; D009844:Oligosaccharides
Sravnitel'noe izuchenie uglevodnykh tsepeĭ H1 gemaggliutinina virusov grippa A/Kiev/59/79 i X/Leningrad/54/1 s pomoshch'iu oligosakharidnykh kart.