The influence of histamine at various concentrations on the cell cycle state of hematopoietic stem cells (CFU-s).
The influence of histamine at various concentrations on the cell cycle state of hematopoietic stem cells (CFU-s) was investigated. CFU-s were triggered from the G0 state into the S phase of the cell cycle by in vitro treatment of mouse bone marrow cells with high concentrations of histamine. This effect could be antagonized by a histamine H2 receptor blocking agent. When bone marrow cells were treated with a histamine H1 receptor antagonist prior to histamine treatment, low concentrations of histamine also triggered the entrance of CFU-s into the DNA synthetic phase. Our findings further suggest the existence of histamine H1 and H2 receptors on the surface of CFU-s cells and the antagonistic effect of these two histamine receptor subtypes on the cell cycle state of CFU-s. Our results also suggest that histamine may participate in regulating the proliferation of hematopoietic stem cells in vivo during immune or inflammatory responses.
Yi
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Research Laboratory of Blood Physiology, Hunan Medical College, Changsha, PRC.
Xu
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YH
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002453:Cell Cycle / Q000187:drug effects; D002927:Cimetidine / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006632:Histamine / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011738:Pyrilamine / Q000494:pharmacology
D002927:Cimetidine; D006632:Histamine; D011738:Pyrilamine