Isolation and pharmacological characterization of a new alpha-neurotoxin (alpha-AgTx) from venom of the viper Agkistrodon halys (Pallas).
A hitherto unknown alpha-neurotoxin, alpha-agkistrodotoxin, was isolated from the venom of the pit viper Agkistrodon halys (Pallas). It's molecular weight was approx. 8000 +/- 80 (SDS-polyacrylamide electrophoresis). The toxin crossreacted with antiserum directed against alpha-bungarotoxin and inhibited binding of 125I-alpha-bungarotoxin to the nicotinic acetylcholine receptor of cultured myotubes (IC50 = 2 X 10(-9) M). The association and dissociation rates were 4.85 X 10(5) per mole per min and 3.55 X 10(-4) per min, respectively, giving a Kd of 7.3 X 10(-10) M. The toxin also inhibited carbachol-induced influx of cations through the nAChR (IC50 = 6 X 10(-8) M).
Unit of Neurochemistry and Neurotoxicology, University of Stockholm, Sweden.
M
M
Jiang
J
J
Häggblad
E
E
Heilbronn
D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D007700:Kinetics; D008970:Molecular Weight; D009498:Neurotoxins / Q000302:isolation & purification* / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000378:metabolism
D002038:Bungarotoxins; D003435:Crotalid Venoms; D009498:Neurotoxins; D011950:Receptors, Cholinergic; C054663:alpha-agkistrodotoxin