Evidence for two conductance/exchange pathways for chloride in rat parotid secretory granules.
Electron probe x-ray microanalysis was used to determine that bromide is localized to rat parotid secretory granules at early stages of an in situ Cl/Br washout experiment. Chloride efflux and bromide influx across the secretory granule membrane occurred with a time order of minutes. Since the Cl washout data indicated minimal Cl binding within the granule, and therefore minimal Br binding, the Br localization results suggest the presence of two or more anion conductance/exchange pathways in the granule membrane for the Cl (Br) ion.
Department of Oral Biology, School of Dentistry, University of Washington, Seattle 98195.
M K
MK
Goddard
K T
KT
Izutsu
D E
DE
Johnson
W Y
WY
Ensign
S M
SM
Izutsu
L E
LE
Wilkinson
S W
SW
Chen
J L
JL
Wong
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000502:physiology*; D004553:Electric Conductivity; D004577:Electron Probe Microanalysis; D008297:Male; D010306:Parotid Gland / Q000166:cytology*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002712:Chlorides; D002217:Carbachol; D011188:Potassium