Selective medium for isolation and enumeration of Bifidobacterium spp.
A new method was developed for the isolation and enumeration of Bifidobacterium spp. from natural aquatic environments. The method was based on the utilization of a new medium, Bifidobacterium iodoacetate medium 25, and resuscitation techniques were used to isolate injured bifidobacteria. The new medium was tested with a nonselective reference medium on sewage and sewage-polluted surface waters. Relatively little colonial growth of any other bacterial genera occurred; when such colonies did grow, Bifidobacterium could be easily differentiated by its colonial morphology or, after Gram staining, by its typical bifidobacterial morphology.
Muñoa
F J
FJ
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Spain.
Pares
R
R
D001644:Bifidobacterium / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media; D012722:Sewage; D014871:Water Microbiology
D003470:Culture Media; D012722:Sewage