Localization of dehydropeptidase-I, an enzyme processing glutathione, in the rat kidney.
An antibody prepared against dehydropeptidase-I, one of the glutathione processing enzymes, from the renal membrane fraction of rats was employed to localize at the light microscopic level the enzyme in the kidney using the avidin/biotin-peroxidase complex method. Dehydropeptidase-I was found to be present on both the brush border and the basolateral membranes of proximal tubular cells. Furthermore, the enzyme activity in the isolated brush border and basolateral membrane fractions was also determined. Distribution profiles of the enzyme activity showed good agreement with the results of the immunohistochemical observations.
Hirota
T
T
Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd., Tokyo.
Nishikawa
Y
Y
Tanaka
M
M
Fukuda
K
K
Igarashi
T
T
Kitagawa
H
H
D000818:Animals; D002498:Centrifugation; D004150:Dipeptidases / Q000302:isolation & purification*; D005978:Glutathione / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D004150:Dipeptidases; C038937:dipeptidase; D005978:Glutathione