Identification and electrophysiological investigations of embryonic mammalian motoneurones in culture.
The retrograde transport of fluorochromes from muscle was used to label embryonic rat motoneurones in vivo. The fluorescent motoneurones were subsequently detected in cultures of dissociated spinal cord neurones using an image-intensification video camera, the images from which allowed identification of the motoneurones under phase-contrast microscopy. Such motoneurones were mostly phase dark, often with a multipolar appearance and were detectable for up to 4 days in culture. Whole-cell patch-clamp recordings from these motoneurones demonstrated mean input resistances of 635 M omega and resting membrane potentials of -65 mV; action potentials could be evoked and, under voltage-clamp, inward and outward currents were present.
Sobell Department of Neurophysiology, Institute of Neurology, London, U.K.
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Fruns
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Krieger
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Sears
D000818:Animals; D001369:Axons / Q000378:metabolism; D001692:Biological Transport; D002478:Cells, Cultured; D004594:Electrophysiology; D005456:Fluorescent Dyes / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology*; D009132:Muscles / Q000378:metabolism; D051381:Rats / Q000196:embryology; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology
D005456:Fluorescent Dyes