Human bone marrow CFU-GM and BFU-E localized by light scatter cell sorting.
Flow cytometric separation was performed on the normal human bone marrow (BM) by using the low-angle (0 degrees) or high-angle (90 degrees) light scatter. Four distinct subpopulations of cells can be enriched from normal human BM and these fractions were subsequently evaluated for their morphological properties as well as their clonogenic capacity in various progenitor cell assays. Our results indicate that human erythroid and granulocyte-macrophage progenitor cells can be separated from BM low-density cells by cell sorting, and these cells show similar 0 degrees and 90 degrees light scatter properties to those observed with murine bone marrow studies. Flow cytometric analysis also suggests that the majority of sorted BFU-E and CFU-GM resides in the blast cell subset of human BM mononuclear cells.
Laboratories of Hematopoietic Cell Kinetics, Memorial Sloan-Kettering Cancer Center, New York, N.Y.
J
J
Atzpodien
S C
SC
Gulati
J H
JH
Kwon
M
M
Wachter
J
J
Fried
B D
BD
Clarkson
D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008027:Light; D008264:Macrophages / Q000166:cytology; D012542:Scattering, Radiation