Free radical scavenging systems and the effect of peroxide damage in aged human skin fibroblasts.
One prominent theory of aging postulates an accumulation of cell damage resulting from nonenzymatic chemical reactions between important cellular components and free radicals. Fibroblast lines derived from skin biopsies of psychiatric patients ranging in age from 22 to 70 were evaluated soon after adaptation to culture. No significant correlation was found between donor age and the detoxification enzyme activities of superoxide dismutase (SOD) or aryl hydrocarbon hydroxylase (AHH) or susceptibility to damage by oxygen metabolites as measured by cell viability or lactate dehydrogenase (LDH) leakage.
Gutman
R L
RL
Ensor Research Laboratory, William S. Hall Psychiatric Institute, University of South Carolina, Columbia 29202.
Cohen
M R
MR
McAmis
W
W
Ramchand
C N
CN
Sailer
V
V
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D005609:Free Radicals; D006801:Humans; D008297:Male; D008875:Middle Aged; D010545:Peroxides / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D010545:Peroxides; D001189:Aryl Hydrocarbon Hydroxylases; D013482:Superoxide Dismutase