Isoelectric focusing on cellulose acetate membrane: a separation procedure for alkaline phosphatase isoenzymes.
An isoelectric focusing technique for the separation of alkaline phosphatase isoenzymes on cellulose acetate membrane is described. Optimal conditions for isoelectric focusing were established by changing ampholine concentration and focusing conditions. Bone, liver, intestinal, and placental isoenzymes can be resolved into various sub-bands in a pH range of 4.1 to 5.2. These sub-bands were correlated with the findings of electrophoretic isoenzyme separation. The whole procedure proves very simple to perform and comparatively time saving (4 h). This procedure may help clarify the problems of ALP isoenzyme differentiation when electrophoretic patterns are unresolved.
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Laboratorio di Chimica Clinica ed Ematologia, Istituti Ospitalieri, Verona, Italia.
Marini
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Rizzotti
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D000293:Adolescent; D000469:Alkaline Phosphatase / Q000097:blood* / Q000302:isolation & purification; D001847:Bone Diseases / Q000175:diagnosis; D002648:Child; D004796:Clinical Enzyme Tests; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D008107:Liver Diseases / Q000175:diagnosis; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D012016:Reference Values
D007527:Isoenzymes; D000469:Alkaline Phosphatase