Ethanol-induced modulation of 35S-TBPS binding to brain membranes.
The effect of ethanol in vitro on the binding of 35S-t-butylbicyclophosphorothionate to brain membranes was examined. Ethanol inhibited basal binding of 35S-TBPS both in potassium bromide- (KBr)- and potassium chloride (KCl)-containing assay media. Ethanol was, however, considerably weaker in reducing 35S-TBPS binding in buffers with 100 mM KCl. Ethanol had no effect on the inhibition of 35S-TBPS binding produced by GABA and pentobarbital in the presence of KBr or KCl, whereas it clearly altered the enhancement of 35S-TBPS binding produced by these drugs when the assay was carried out in buffers containing KCl.
Laboratory for Studies of Neuroadaptive Processes, NIAAA, Bethesda, MD 20892.
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Liljequist
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Culp
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Tabakoff
D000818:Animals; D001921:Brain / Q000187:drug effects*; D001643:Bridged Bicyclo Compounds / Q000493:pharmacokinetics*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D001952:Bridged-Ring Compounds / Q000493:pharmacokinetics*; D002462:Cell Membrane / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D001952:Bridged-Ring Compounds; D000431:Ethanol; C037476:tert-butylbicyclophosphorothionate