Enzyme-linked immunosorbent assay for canine serum ferritin, using monoclonal anti-canine ferritin immunoglobulin G.
Immunoassay of serum ferritin is currently used to evaluate the clinical iron status of human beings, horses, cattle, and swine. Because ferritins are immunologically species specific, a separate assay must be developed for each species. We have developed an ELISA for serum ferritin in dogs, using a monoclonal anti-canine ferritin antibody. Ferritin standards were linear (r = 0.997) from 0 to 80 ng/ml. Recovery of ferritin from canine serum was 94%. Dilutions of pooled canine serum were linear from 0 to 50% (r = 0.994). Within-assay coefficient of variability was 5.5%, whereas assay-to-assay coefficient of variability ranged from 12.5 to 21%. This assay should provide a nonsurgical means of accurately estimating dogs' iron stores.
Weeks
B R
BR
Department of Veterinary Pathology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
Smith
J E
JE
Phillips
R M
RM
D000818:Animals; D000911:Antibodies, Monoclonal; D004285:Dogs / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005293:Ferritins / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D005293:Ferritins