Measurements of the specific activity of the nucleoside triphosphate pool of sea-urchin embryos following 8-3H-guanosine administration.
The nucleoside triphosphate (NTP) pool during the early development of sea-urchin, Paracentrotus lividus, was measured using high-performance liquid chromatography (HPLC). Each NTP pool was measured at four stages between the eight-blastomere stage and the early blastula stage, as was the specific activity of each NTP following an initial administration of 8-3H-guanosine. Unexpectedly high UTP (uridine-5'-triphosphate) concentrations and radioactivity levels in UTP were observed. Studies of the sea-urchin, Strongilocentrotus purpuratus, also revealed elevated levels of 8-3H-guanosine incorporation into UTP. The present procedure was found to be suitable for the unequivocal identification of UTP.
Arezzo
F
F
Department of Cellular and Developmental Biology, University of Palermo, Italy.
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D001757:Blastomeres / Q000378:metabolism; D002454:Cell Differentiation; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000502:physiology; D006151:Guanosine / Q000378:metabolism*; D007700:Kinetics; D009028:Morula / Q000378:metabolism; D012265:Ribonucleotides / Q000096:biosynthesis*; D012617:Sea Urchins / Q000196:embryology*; D014316:Tritium
D012265:Ribonucleotides; D014316:Tritium; D006151:Guanosine