Glycidol-modified gels for molecular-sieve chromatography. Surface hydrophilization and pore size reduction.
Divinyl sulfone-crosslinked agarose gels were made hydrophilic by coupling glycidol to the agarose chains. The concentration of glycidol in the reaction mixture determines the pore size of the gels (the glycidol molecules probably form polymers, the degree of polymerization increasing with the glycidol concentration). Gels prepared with moderate glycidol concentrations are still porous enough to be used for separation of proteins and peptides. Gels with a high degree of glycidol polymerization are suited for desalting of low-molecular-weight compounds, for instance peptides.
Eriksson
K O
KO
Institute of Biochemistry, Biomedical Center, University of Uppsala, Sweden.
D000433:1-Propanol / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004852:Epoxy Compounds / Q000032:analysis*; D004988:Ethers, Cyclic / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D020005:Propanols; D011506:Proteins / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D010455:Peptides; D020005:Propanols; D011506:Proteins; D000433:1-Propanol; C004312:glycidol