Alkaline extraction and reverse-phase high-performance liquid chromatography of adenine and pyridine nucleotides in human platelets.
The levels of adenine (ATP, ADP, AMP) and pyridine (NAD, NADH) nucleotides in human platelets have been measured by a simple and reproducible method. A rapid alkaline extraction allows a complete recovery of the compounds concerned. The metabolic ATP and ADP in the cytosolic fraction, the amount released upon thrombin stimulation, and the ADP bound to F-actin have also been evaluated. Analysis was performed by reverse-phase, isocratic high-performance liquid chromatography on a 5-microns Lichrosorb RP-18 column with uv detection at 254 nm.
Institute of Biological Chemistry, University of Genoa, Italy.
G
G
Leoncini
E
E
Buzzi
M
M
Maresca
M
M
Mazzei
A
A
Balbi
D000227:Adenine Nucleotides / Q000097:blood* / Q000302:isolation & purification; D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001792:Blood Platelets / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009243:NAD / Q000097:blood* / Q000302:isolation & purification; D009249:NADP / Q000097:blood; D010084:Oxidation-Reduction
D000227:Adenine Nucleotides; D009243:NAD; D000249:Adenosine Monophosphate; D009249:NADP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate