Characterization and quantification of PAF-acether (platelet-activating factor) as a heptafluorobutyrate derivative of 1-O-alkyl-2-acetyl-sn-glycerol by capillary column gas chromatography with electron-capture detection.
A gas chromatographic method with a glass capillary column and electron-capture detection is proposed for the characterization and quantification of 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine (PAF C16) using the corresponding 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-heptafluorobutyrate derivative and, as an internal standard, 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine. The reproducibility was approximately 5% and amounts as low as 20 pg could be measured. The method was specific and allowed the quantification of PAF C16 in supernatants from stimulated human polymorphonuclear neutrophils.
Bossant
M J
MJ
Laboratoire de Chimie Analytique II, S.A.M.M., Faculté de Pharmacie, Chatenay Malabry, France.
Farinotti
R
R
Mencia-Huerta
J M
JM
Benveniste
J
J
Mahuzier
G
G
D002849:Chromatography, Gas; D004563:Electrochemistry; D005466:Fluorocarbons; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D009504:Neutrophils / Q000032:analysis; D010972:Platelet Activating Factor / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis; D014280:Triglycerides / Q000032:analysis
D005466:Fluorocarbons; D007202:Indicators and Reagents; D010972:Platelet Activating Factor; D014280:Triglycerides; C055317:1-O-hexadecyl-2-O-acetyl-sn-glycero-3-heptafluorobutyrate; C033094:perfluorobutyric acid