Rapid assessment of microspore and pollen development stage in wheat and maize using DAPI and membrane permeabilization.
The use of the DNA-specific fluorochrome DAPI has been extended to stage assessment of fresh pollen in wheat and maize. Membrane permeabilization by Triton X-100 incorporated in the staining solution allows access of the fluorochrome to nuclear DNA. At all stages of gametophytic development, the nuclei can be sharply visualized. Starch does not interfere with the fluorochrome so that it is possible to study the second pollen grain mitosis and sperm differentiation. With its rapidity and reliability, this technique represents an efficient tool for routine staging or investigation of the nuclear status of the pollen grains.
Cell Recognition and Plant Breeding Laboratory, University of Lyon I, Villeurbanne, France.
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Vergne
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Delvallee
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Dumas
D002463:Cell Membrane Permeability; D003902:Detergents; D005456:Fluorescent Dyes; D007211:Indoles; D017830:Octoxynol; D059828:Plant Cells; D063245:Plant Development; D011058:Pollen / Q000648:ultrastructure*; D011092:Polyethylene Glycols; D013045:Species Specificity; D013170:Spores / Q000648:ultrastructure; D014908:Triticum / Q000166:cytology / Q000254:growth & development; D003313:Zea mays / Q000166:cytology / Q000254:growth & development
D003902:Detergents; D005456:Fluorescent Dyes; D007211:Indoles; D011092:Polyethylene Glycols; C007293:DAPI; D017830:Octoxynol