Determination of hydrogen peroxide in beverages by high-performance liquid chromatography with fluorescence detection.
A high-performance liquid chromatographic method is described for the determination of hydrogen peroxide in beverages. The method involves the enzymatic conversion of hydrogen peroxide into formaldehyde by catalase-methanol, followed by the derivatization of formaldehyde with 4-amino-3-penten-2-one. The reaction was carried out under the flow of nitrogen, which allows the detection of hydrogen peroxide without interference from reducing substances such as ascorbic acid. More than 90% of hydrogen peroxide spiked at the 2 ppm level was recovered from several beverages. The method exhibits good linearity, accuracy and precision, and the minimum detectable level was 0.05 ppm.
Public Health Research Institute of Kobe City, Japan.
T
T
Hamano
Y
Y
Mitsuhashi
S
S
Yamamoto
D001205:Ascorbic Acid / Q000032:analysis; D001628:Beverages / Q000032:analysis*; D002374:Catalase; D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D000432:Methanol / Q000032:analysis; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D006861:Hydrogen Peroxide; D002374:Catalase; D001205:Ascorbic Acid; D000432:Methanol