Occurrence of a cobalt-induced and cobalt-containing nitrile hydratase in Rhodococcus rhodochrous J1.
The formation of nitrile hydratase required cobalt ions in Rhodococcus rhodochrous J1. No other transition-metals could replace the cobalt ion. The Rhodococcus nitrile hydratase was purified to homogeneity and found to contain a cobalt atom. The occurrence of a cobalt-induced and cobalt-containing nitrile hydratase, different from the nitrile hydratases in Pseudomonas chlororaphis B23 and Brevibacterium R312 containing a ferric ion in their active center, has been demonstrated here for the first time.
Department of Agricultural Chemistry, Kyoto University, Japan.
T
T
Nagasawa
K
K
Takeuchi
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H
Yamada
D001951:Brevibacterium / Q000201:enzymology; D003035:Cobalt / Q000032:analysis / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006836:Hydro-Lyases / Q000032:analysis*; D011549:Pseudomonas / Q000201:enzymology; D012240:Rhodococcus / Q000201:enzymology*
D003035:Cobalt; D006836:Hydro-Lyases; C048831:nitrile hydratase