Genetics of esterases in Drosophila. IX. Characterization of the JH-esterase in D. virilis.
The kinetic characteristics of the main isozymes of Drosophila virilis esterase were studied and Km values of esterase-2, -4, and -6 and p-esterase for alpha- and beta-naphthyl acetate were obtained. Juvenile hormone (JH) was shown to inhibit the p-esterase activity when in competition with beta-naphthyl acetate and the general esterase inhibitor, diisopropylphosphofluoridate (DFP), was shown to inhibit all the components of the D. virilis esterase patterns except p-esterase. While studying the changes of p-esterase activity in D. virilis ontogenesis, the increase in p-esterase activity in the wandering larvae, prepupae, and early pupae was found to correlate with a decrease in JH titer at these stages. The decrease in JH level in a temperature-sensitive lethal mutant larvae of D. virilis at high temperatures was shown to correlate with increased p-esterase activity. These results confirm that p-esterase of D. virilis is JH-esterase.
Rauschenbach IYu
Institute of Cytology and Genetics, Siberian Department, USSR Academy of Sciences, Novosibirsk.
Lukashina
N S
NS
Budker
V G
VG
Korochkin
L I
LI
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D004950:Esterases / Q000235:genetics*; D006358:Hot Temperature; D006868:Hydrolysis; D007527:Isoenzymes / Q000235:genetics*; D009154:Mutation; D009284:Naphthols / Q000378:metabolism
D007527:Isoenzymes; D009284:Naphthols; C005366:naphthyl acetate; D004950:Esterases; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase