[Partial purification of tissue thromboplastin by the gel filtration method and its use for reproducing the generalized decompensated thrombinogenesis syndrome].
Tissue thromboplastin was purified using various chromatographic resins: Sephadex G-200, Ultragel AcA 34, Acrylex P-300 and Toyopearl HW-55F. The purity of the preparations obtained was evaluated by means of the ratio of the thromboplastin solutions absorbency at 540 nm and a protein concentration in the solution expressed as mg/ml. The most purified preparations of tissue thromboplastin were obtained after gel filtration on Toyopearl HW-55F. The thromboplastin preparation produced was used for experimental reproduction of the syndrome of generalized decompensated thrombinogenesis.
Magerovskiĭ
Iu V
IuV
Monastyrskiĭ
V A
VA
Gaĭda
A V
AV
Birka
I I
II
Voroniak
M I
MI
D000818:Animals; D001780:Blood Coagulation Tests; D002850:Chromatography, Gel; D004211:Disseminated Intravascular Coagulation / Q000097:blood* / Q000473:pathology; D006801:Humans; D007668:Kidney / Q000648:ultrastructure; D008297:Male; D009206:Myocardium / Q000648:ultrastructure; D051381:Rats; D013925:Thromboplastin / Q000302:isolation & purification* / Q000502:physiology
D013925:Thromboplastin
Chastichnaia ochistka tkanevogo tromboplastina metodom gel'-pronikaiushcheĭ khromatografii i vosproizvedenie s ego pomoshch'iu sindroma generalizovannogo dekompensirovannogo trombinogeneza.