Rose bengal-induced photodamage to subcellular membranes and enzymes.
Studies were made of photodamage (photolysis) caused by Rose Bengal + light of peroxisomes, mitochondria, and lysosomes, as well as inhibition of the respective marker enzymes: catalase, sulfite oxidase, and acid phosphatase. The time-dependence of lipid peroxidation caused by the same system and measured by the increase of thiobarbituric acid-reactive substances was also examined. The most sensitive to photolysis were the peroxisomes and the least susceptible were lysosomes as the inhibition of their marker enzymes followed this order. The sensitivity of the subcellular membranes to digitonin (a sterol binding agent) showed the opposite dependence. The importance of the cholesterol content for the membrane stability to active oxygen species, as well as the role of lipid peroxidation in photodynamic damage of the systems studied, is discussed.
Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
N
N
Radonova
S
S
Liochev
E
E
Russanov
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000528:radiation effects; D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors / Q000528:radiation effects; D007425:Intracellular Membranes / Q000187:drug effects / Q000528:radiation effects*; D008027:Light; D008054:Lipid Peroxides / Q000096:biosynthesis; D008247:Lysosomes / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000528:radiation effects; D008928:Mitochondria / Q000187:drug effects / Q000528:radiation effects; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000037:antagonists & inhibitors / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D012395:Rose Bengal / Q000633:toxicity*; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*
D008054:Lipid Peroxides; D012395:Rose Bengal; D002374:Catalase; D050862:Oxidoreductases Acting on Sulfur Group Donors; D000135:Acid Phosphatase