SDS-PAGE analysis of the protein layers adsorbing in vivo and in vitro to bone substituting materials.
The composition of the protein layer adsorbed to the bone substituting materials, hydroxyapatite, beta-whitlockite, titanium and aluminium, in vivo (intramuscularly in guinea pig) and in vitro, was investigated using SDS-gel electrophoresis (SDS-PAGE). After in vivo implantation for 1 d mainly proteins with molecular weights between 10,000 and 20,000 were adsorbed. After 3 months the biolayer of the implanted biomaterials also contained proteins with molecular weights 35,000, 45,000, 60,000 and 200,000. No large qualitative differences in protein composition of the biolayers on the various implanted materials were found. In vitro incubation with human serum resulted in binding of proteins with estimated molecular weights of 30,000, 60,000 (albumin), 200,000 and greater than 200,000. It is suggested that the differences between in vivo and in vitro protein adsorption are due to proteolysis occurring in vivo in the vicinity of the implanted material.
Department of Biochemistry, Vrije Universiteit, Amsterdam, The Netherlands.
E C
EC
Veerman
R J
RJ
Suppers
C P
CP
Klein
K
K
de Groot
A V
AV
Nieuw Amerongen
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D001798:Blood Proteins / Q000032:analysis; D001842:Bone and Bones; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006168:Guinea Pigs; D006801:Humans; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*
D001672:Biocompatible Materials; D001798:Blood Proteins; D011506:Proteins