Accumulation of large neutral amino acids in the brain of sparse-fur mice at hyperammonemic state.
Sparse-fur mice which are deficient in ornithine transcarbamylase, the second-step enzyme in the urea cycle, were examined for hyperammonemia and its relationship with encephalopathy. We compared amino acid concentrations in the serum and brain of spf mice with those of control mice. Unlike hepatic encephalopathy we could not find marked amino acid changes in the serum of spf mice besides low levels of citrulline and arginine. But in the brain of spf mice, glutamine was increased strikingly during hyperammonemia, and a concomitant accumulation of large neutral amino acids such as tyrosine, phenylalanine, methionine, and histidine was observed. The accumulation of these large neutral amino acids in the brain was not influenced by 24-hr fasting which caused increases in branched chain amino acids in the serum. From these results, we conclude that the accumulation of the large neutral amino acid in the brain of hyperammonemic state is caused by uptake of ammonia in the brain and the subsequent accumulation of glutamine, but is not influenced by a decreased ratio of branched chain amino acids to aromatic amino acids in the serum.
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
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Inoue
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Gushiken
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Kobayashi
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Saheki
D000375:Aging / Q000097:blood; D000596:Amino Acids / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D000641:Ammonia / Q000097:blood*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D051379:Mice
D000596:Amino Acids; D000641:Ammonia