Stability of protein and mRNA in human postmortem liver--analysis by two-dimensional gel electrophoresis.
The method of two-dimensional polyacrylamide gel electrophoresis has been used to investigate the post mortem stability of protein and mRNA in human liver. The electrophoretic mobility of proteins and of the in vitro translation products of the mRNA were found to be essentially unaffected by incubation of the liver at 37 degrees C for up to 2 h or at 4 degrees C for up to 16 h. This study indicates that the major protein and mRNA species in liver are stable enough following death to allow meaningful studies on tissue collected under standard autopsy conditions.
Murdoch Institute for Research into Birth Defects, Royal Children's Hospital, Parkville, Victoria, Australia.
J M
JM
Finger
J F
JF
Mercer
R G
RG
Cotton
D M
DM
Danks
D000293:Adolescent; D001344:Autopsy; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008099:Liver / Q000032:analysis*; D008297:Male; D011506:Proteins / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*
D011506:Proteins; D012333:RNA, Messenger