First derivative spectrophotometric determination of anhydrotetracyclines in tetracyclines.
A rapid method is presented for detection and determination of anhydrotetracycline-HCl (ATC-HCl) and 4-epianhydrotetracycline-HCl (4-EATC-HCl) in tetracycline-HCl (TC-HCl). The method determines the 2 compounds as a sum, not individually. The first derivative absorption curve has a trough (D1) at 460 nm which is linearly related to concentration (1-10 mg/mL). ATC-HCl + 4-EATC-HCl content was determined in TC-HCl powder and capsules by the D1 and the compensation D1 spectrophotometric methods. The results were compared with those obtained using U.S. Pharmacopeia and British Pharmacopoeia methods.
Wahbi
A A
AA
King Saud University, College of Pharmacy, Pharmaceutical Chemistry Department, Riyadh, Saudi Arabia.
Al-Khamees
H A
HA
Yousef
A M
AM
D055598:Chemical Phenomena; D002621:Chemistry; D002751:Chlortetracycline / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013752:Tetracycline / Q000032:analysis*; D013754:Tetracyclines / Q000032:analysis*
D013754:Tetracyclines; C016229:4-epianhydrotetracycline; D013752:Tetracycline; D002751:Chlortetracycline