[Isolation, polypeptide composition and properties of aminoacyl-tRNA-synthetase complexes from the rabbit liver].
The procedure for isolation of the aminoacyl-tRNA-synthetase complex from rabbit liver based on the affinity chromatography on heparin- and tRNA-Sepharose has been developed. The complex has a Mr of about 1100 kD and is made up of 10 polypeptides, eight of which are aminoacyl-tRNA synthetase. The complex stability was studied under various conditions. The data obtained are discussed in terms of the involvement of hydrophobic domains of aminoacyl-tRNA synthetases in the stabilization of the complex.
A D
AD
Vol'fson
Iu A
IuA
Motorin
A F
AF
Orlovskiĭ
K L
KL
Gladilin
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004795:Enzyme Stability; D007525:Isoelectric Focusing; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis*; D011817:Rabbits
D009097:Multienzyme Complexes; D010455:Peptides; D000604:Amino Acyl-tRNA Synthetases
Vydelenie, polipeptidnyĭ sostav i svoĭstva kompleksa aminoatsil-tRNA-sintetaz iz pecheni krolika.