Internal standards for quantitative gas chromatography of individual bile acids after group separation of bile acids in urine.
Sodium glyco-7 alpha,12 alpha-dihydroxy-5 beta-cholan-24-oate, sodium tauro-7 beta,12 beta-dihydroxy-5 beta-cholan-24-oate and disodium glyco-7 alpha,12 alpha-dihydroxy-5 beta-cholan-24-oate 7 alpha-sulphate have been synthesized for the first time. These compounds, together with 7 beta,12 beta-dihydroxy-5 beta-cholan-24-oic acid, which were added to a sample prior to extraction, were found to be useful as internal standards for determination by gas chromatography of individual bile acids in each fraction after group separation of urinary bile acids.
Division of Biochemistry, Tottori University School of Medicine, Yonago, Japan.
N
N
Yamaga
K
K
Adachi
H
H
Kohara
K
K
Shimizu
D001647:Bile Acids and Salts / Q000138:chemical synthesis / Q000652:urine*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D012015:Reference Standards
D001647:Bile Acids and Salts