Development of a simple, fluoroimmunoassay for paraquat.
High affinity populations of antibodies to paraquat were produced in high concentrations in the sera of immunised sheep. A simple fluoroimmunoassay was developed for rapid measurement of plasma paraquat. Fluorescein-labelled paraquat was added to sample or standard (20 microliter) followed by premixed first and second antisera. Equilibrium was reached within 2 min and results were available within 20 min. Correlation with weighed-in paraquat gave a regression line (r) of 0.997. All reagents proved stable and are suitable for general distribution.
Department of Chemical Pathology, St Bartholomew's Hospital, London, England, UK.
R E
RE
Coxon
C
C
Rae
G
G
Gallacher
J
J
Landon
D000818:Animals; D000917:Antibody Formation; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins; D006801:Humans; D007118:Immunoassay / Q000379:methods; D010269:Paraquat / Q000097:blood*; D012756:Sheep / Q000276:immunology
D005452:Fluoresceins; D010269:Paraquat; D019793:Fluorescein