HL-60 cell line was derived from a patient with FAB-M2 and not FAB-M3.
The leukemia from which the human cell line HL-60 was derived was classified in 1976 as acute progranulocytic leukemia (APL), although it was recognized to show a number of atypical features. In the ensuing 10 years, the concept of APL and its integral association with t(15;17) has evolved, and the concept of APL as a morphologically recognizable entity has become embodied in the term French-American-British classification M3 (FAB-M3). It is now recognized that not every case of leukemia with a high proportion of progranulocytes can be classified as FAB-M3. We reviewed the light and ultrastructural morphology of the original diagnostic material from this case, and we report that the leukemia from which HL-60 was derived does not conform to the currently recognized entity of FAB-M3 and is more appropriately classified as an acute myeloblastic leukemia with maturation, FAB-M2.
Division of Laboratory Medicine, University of Texas M.D. Anderson Hospital and Tumor Institute at Houston 77030.
W T
WT
Dalton
M J
MJ
Ahearn
K B
KB
McCredie
E J
EJ
Freireich
S A
SA
Stass
J M
JM
Trujillo
D000328:Adult; D002460:Cell Line; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000145:classification* / Q000648:ultrastructure