The expression of Cellulomonas fimi cellulase genes in Brevibacterium lactofermentum.
The exoglucanase gene (cex) and the endoglucanase A gene (cenA) from Cellulomonas fimi were subcloned into the Escherichia coli/Brevibacterium lactofermentum shuttle vector pBK10. Both genes were expressed to five to ten times higher levels in B. lactofermentum than in E. coli, probably because these genes were expressed from C. fimi promoters. In B. lactofermentum virtually all of the enzyme activities were in the culture supernatant. This system will facilitate analysis of the expression of the C. fimi genes in and secretion of their products from a Gram-positive bacterium.
Department of Microbiology, University of British Columbia, Vancouver, Canada.
F W
FW
Paradis
R A
RA
Warren
D G
DG
Kilburn
R C
RC
Miller
D001951:Brevibacterium / Q000235:genetics*; D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006094:Gram-Positive Bacteria / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
D002480:Cellulase