Age-dependent changes in the osmotic behavior of rat brain and liver.
Osmotic potential of liver and brain tissue has been determined by measuring swelling or shrinkage of the tissues in anoxic Ringer solution the osmotic concentration of which was rendered hypo- or hyperosmotic by dilution or addition of polyethylene glycol (PEG 6000), respectively. The percentages of volume change were fitted to an exponential equation permitting the calculation of the initial speed of volume change. The age-dependent increase of the intracellular dry mass content was also taken into consideration. It has been established that initial velocity of the volume change displays an age-dependent decline in all kinds of media tested; however, the slope of this decrease is steeper in the diluted Ringer solution than in the other ones. Calculations show that the colloid osmotic pressure (mmHg) of the intracellular mass decreases between 1 and 26 months of age from 810 to 596 and from 2,477 to 904 in the brain and liver, respectively. The results may be interpreted as consequences of a decreased colloid dispersity which may be related to an oxygen free radical induced intermolecular cross-linking.
F. Verzár International Laboratory for Experimental Gerontology, University Medical School, Debrecen, Hungary.
I
I
Zs-Nagy
B
B
Derecskei
G
G
Lustyik
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism*; D009995:Osmosis; D051381:Rats