Multiple functional motifs in the chicken U1 RNA gene enhancer.
The DNA sequence requirements of chicken U1 RNA gene expression have been examined in an oocyte transcription system. An enhancer region, which was required for efficient U1 RNA gene expression, is contained within a region of conserved DNA sequences spanning nucleotide positions -230 to -183, upstream of the transcriptional initiation site. These DNA sequences can be divided into at least two distinct subregions or domains that acted synergistically to provide a greater than 20-fold stimulation of U1 RNA synthesis. The first domain contains the octamer sequence ATGCAAAT and was recognized by a DNA-binding factor present in HeLa cell extracts. The second domain (the SPH domain) consists of conserved sequences immediately downstream of the octamer and is an essential component of the enhancer. In the oocyte, the DNA sequences of the SPH domain were able to enhance gene expression at least 10-fold in the absence of the octamer domain. In contrast, the octamer domain, although required for full U1 RNA gene activity, was unable to stimulate expression in the absence of the adjacent downstream DNA sequences. These findings imply that sequences 3' of the octamer play a major role in the function of the chicken U1 RNA gene enhancer. This concept was supported by transcriptional competition studies in which a cloned chicken U4B RNA gene was used to compete for limiting transcription factors in oocytes. Multiple sequence motifs that can function in a variety of cis-linked configurations may be a general feature of vertebrate small nuclear RNA gene enhancers.
Roebuck
K A
KA
Department of Chemistry, San Diego State University, California 92182.
Walker
R J
RJ
Stumph
W E
WE
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006367:HeLa Cells; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D012342:RNA, Small Nuclear; D004247:DNA