Application of preparative reversed-phase high-performance liquid chromatography to the isolation of insulin-like growth factor II from human serum.
Substantially purified insulin-like growth factor II (IGF-II) was prepared from human serum. Initial enrichment using ion-exchange chromatography on DEAE Sephadex A50, followed by gel permeation chromatography on Sephadex G-75 in 1% formic acid produced material suitable for application to a preparative reversed-phase high-performance liquid chromatographic (HPLC) column containing LiChroprep RP-18. The latter step gave about 90-fold purification with a recovery of about 70% IGF-II bio-activity. Finally, a small reversed-phase HPLC column achieved a 17-fold purification with similar yield of activity. Overall, the four steps gave IGF-II of about 90% purity in yield of 12%.
Department of Applied Chemistry, Royal Melbourne Institute of Technology, Australia.
H J
HJ
Cornell
P H
PH
Brady
D000328:Adult; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000097:blood*; D007525:Isoelectric Focusing; D013002:Somatomedins / Q000097:blood*
D013002:Somatomedins; D007335:Insulin-Like Growth Factor II