Determination of nitrosamines by liquid chromatography with post-column photolysis and electrochemical detection.
The liquid chromatographic detection of nitrosamines at the ppb (10(9)) and sub-ppb levels is still a difficult problem. A significant improvement was achieved when a nitrosamine eluted from the column was photolysed by a UV lamp, the charged nitrite species being detected amperometrically. N-Nitrosodimethylamine (NDMA) was selected as a model solute to test the applicability of the detection device. The calibration graphs were linear over a wide concentration range. Optimal detection depended on the nature of the buffer used and the UV spectrum of the nitrosamine to be detected. An interesting application of the detection of a high-molecular-weight nitrosamine is given as an example.
Righezza
M
M
Laboratoire de Chimie Appliquée et Génie Chimique, Université Aix-Marseille III, France.
Murello
M H
MH
Siouffi
A M
AM
D001515:Beer / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004128:Dimethylnitrosamine / Q000032:analysis; D004563:Electrochemistry; D009602:Nitrosamines / Q000032:analysis*; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet
D009602:Nitrosamines; D004128:Dimethylnitrosamine