Characterization of DNA binding proteins released from sarcoma-180 chromatin during brief digestion with DNase-I.
Brief digestion of sarcoma-180 chromatin (less than or equal to 5%) by pancreatic DNase-I releases six non-histone proteins with Mol. wt. 21.5K, 26K, 72.5K, 77K, 90K and 120K dalton and pI values ranging from 4.7 to 12.4. The protein of Mol. wt. 77K dalton was obtained at high alkaline range of pH = 12.4. The antibodies against these proteins induce dose dependent inhibition in transcription of native chromatin. Results suggest a role of these proteins in positive control of gene transcription in sarcoma-180 cells.
Department of Biochemistry, Chittaranjan National Cancer Research Centre, Calcutta, India.
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Panda
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Choudhury
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D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000502:physiology; D003850:Deoxyribonuclease I / Q000494:pharmacology*; D012319:RNA Polymerase II / Q000502:physiology; D012510:Sarcoma 180 / Q000378:metabolism*; D014158:Transcription, Genetic
D002843:Chromatin; D004268:DNA-Binding Proteins; D012319:RNA Polymerase II; D003850:Deoxyribonuclease I