Species-specific immunodiagnosis of Taenia solium cysticercosis by ELISA and immunoblotting.
A combination of ELISA and immunoblotting was evaluated for immunodiagnosis of human T. solium cysticercosis. The sensitivity of ELISA for diagnosis of European, Latin-American, Asian and South African patients with cysticercosis was 75% for serum and 55% for cerebrospinal fluid specimen. Antigens originating from cysticerci in pigs from Mexico and South Africa were of adequate applicability. A species-specific confirmation of T. solium cysticercosis by immunoblotting (detection of antibody activity to the 26 K and 8 K bands) was achieved in 92% (serum) and 100% (CSF) of samples positive in ELISA. No antibodies from 147 patients with other helminth infections reacted with the 26 K and the 8 K band in immunoblotting, resulting in a specificity of 100%.
Institute of Parasitology, University of Zürich, Switzerland.
B
B
Gottstein
D
D
Zini
P M
PM
Schantz
D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis* / Q000134:cerebrospinal fluid; D001208:Asia; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005060:Europe; D005260:Female; D006801:Humans; D007118:Immunoassay; D007843:Latin America; D008297:Male; D008800:Mexico; D013019:South Africa; D013045:Species Specificity; D013621:Taenia / Q000276:immunology*; D014481:United States
D000909:Antibodies, Helminth