Effects of hachimijiogan, tokishakuyakusan and keishibukuryogan on progesterone secretions by corpus luteum.
27-day-old rats received 20 IU of PMS subcutaneously, and 56 hours later, 40 IU of hCG to induce superovulated ovaries. Seven days after the hCG treatment, the ovaries were resected and incubated or perifused with an extract of Hachimijiogan (TJ-7), Tokishakuyakusan (TJ-23) or Keishibukuryogan (TJ-25). Progesterone in media was assayed by radioimmunoassay. In the incubation study, TJ-23 significantly (p less than 0.001, p less than 0.05) increased progesterone concentrations, while TJ-7 or TJ-25 showed no remarkable effect. In the perifusion study, TJ-23 or TJ-25 augmented progesterone levels, whereas TJ-7 revealed no effect. These results suggest that TJ-23 or TJ-25 stimulates the corpus luteum to secrete progesterone.
Usuki
S
S
Department of Obstetrics and Gynecology, University of Tsukuba, Ibaraki, Japan.
D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D066298:In Vitro Techniques; D010477:Perfusion; D010936:Plant Extracts / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D010936:Plant Extracts; C054591:hachimijiogan; C054593:keishibukuryogan; C413220:toki-shakuyaku-san; D011374:Progesterone