High-performance liquid chromatographic quantitation of triacylglycerols containing fenoprofen from biological samples.
A normal-phase high-performance liquid chromatographic (HPLC) method has been developed for the quantitation of radiolabelled triacylglycerols containing fenoprofen, synthesized from [3H]glycerol by isolated hepatocytes and adipocytes. The assay consists of extracting the lipids into diethyl ether, separating triacylglycerols from polar endogenous lipids using silica Sep-Pak cartridges and quantitating endogenous triacylglycerols and triacylglycerols containing fenoprofen by HPLC resolution and scintillation counting. HPLC separation is achieved in less than 10 min. Using [14C]tripalmitin as internal standard the assay has a linear relationship between added triacylglycerol and measured endogenous triacylglycerols and triacylglycerols containing fenoprofen with regression coefficients of 0.997 and 0.998, respectively.
Department of Clinical Pharmacology, Flinders Medical Centre, Bedford Park, Australia.
B C
BC
Sallustio
P J
PJ
Meffin
M
M
Thompson
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005279:Fenoprofen / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000032:analysis / Q000166:cytology; D008297:Male; D010666:Phenylpropionates / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000032:analysis*
D008055:Lipids; D010666:Phenylpropionates; D014280:Triglycerides; D005279:Fenoprofen