Enhanced expression of Harvey-ras oncogene in FANFT-induced transitional cell carcinoma.
The level of mRNA expression and DNA structure of c-H-ras were examined in a transitional cell carcinoma (TCC) induced in C3H/He mice and Fisher rats by N-(4,5-nitro-2-furylthiazolyl)formamide (FANFT) and carried as serially transplanted tumors. Neither amplification nor gross rearrangements were detected by Southern analysis in the FANFT tumor. Increased expression of c-H-ras was detected by Northern and in situ hybridization in both the FANFT-induced mouse and rat TCC, as compared to normal urothelium. No expression of six other cellular oncogenes was detected in the tumor. It appears that the increase in H-ras expression in the FANFT tumor is specific to this oncogene and that increased expression of the H-ras gene may play a role in bladder carcinogenesis.
Department of Urology, College of Physicians and Surgeons, Columbia University, New York, N.Y.
I S
IS
Sawczuk
W
W
Walsh
W
W
King
C A
CA
Olsson
C
C
Nguyen-Huu
D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000139:chemically induced / Q000235:genetics*; D005200:FANFT; D005786:Gene Expression Regulation; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009857:Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000235:genetics*
D012333:RNA, Messenger; D005200:FANFT