Induction of lymphocyte agglutination and lysis by Newcastle disease virus.
Five strains of Newcastle disease virus (NDV) were tested for their ability to agglutinate and lyse chicken lymphocytes. All 5 strains agglutinated lymphocytes, and the agglutination was inhibited by anti-NDV sera. All 5 strains also caused a different degree of direct virus-induced lysis of 51Cr-labeled chicken lymphocytes. This direct lymphocyte cytotoxicity was lost after heating at 56 degrees C for 30 min, or after treatment with a high concentration of formalin (1:100 dilution). Neither a low concentration of formalin (1:5000) nor trypsin treatment had any effect on cytotoxicity.
Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
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D000371:Agglutination; D000818:Animals; D002645:Chickens; D005557:Formaldehyde / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006387:Hemagglutination, Viral; D006358:Hot Temperature; D007106:Immune Sera / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D009522:Newcastle disease virus / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology; D014774:Virulence
D007106:Immune Sera; D005557:Formaldehyde; D014357:Trypsin