Analysis of platinum species originating from cis-diamminedichloroplatinum(II) (cisplatin) in human and rat plasma by high-performance liquid chromatography with on-line inductively coupled plasma atomic emission spectrometric detection.
The applicability of reversed-phase ion-pair chromatography with on-line inductively coupled plasma atomic emission spectrometric detection was investigated for the analysis of cisplatin, its hydrolysis products and two methionine-platinum complexes in aqueous solutions. The detector response for cisplatin was linear over three orders of magnitude. The detection limit was found to be 3.5 ng, corresponding to a concentration detection limit of 35 ng/ml. The ion-pair system developed was applied to the analysis of (bio)transformation products originating from cisplatin in human and rat plasma in vitro and in vivo. In vitro, a number of platinum-containing species are formed, two of which show the same retention as the mono- and diaqua hydrolysis products of cisplatin. Preliminary results for in vivo experiments indicate cisplatin to be the predominant species in rat plasma.
Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
W A
WA
de Waal
F J
FJ
Maessen
J C
JC
Kraak
D000097:Acetonitriles; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009944:Organoplatinum Compounds / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards; D012997:Solvents; D013045:Species Specificity; D013054:Spectrophotometry, Atomic; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D009944:Organoplatinum Compounds; D012997:Solvents; D002945:Cisplatin