Thrombosis and blood cells in atherosclerosis development.
Hemorheological changes produced in blood cells seem to be essential in atheroma plaque development and thrombotic episodes. In this study, we investigated the relationship between blood cells count, thrombogenic situations and morphological mesenteric alterations in atherosclerotic rats. Atherosclerosis was induced by an atherogenic diet made up of two phases, the first a hypervitaminic diet, and the second a hyperlipidic one. Cell counts were performed with Thoma's camera. Morphological changes were observed directly in rat mesentery. Thrombogenic situations were investigated by a mesenteric microthrombosis induction method. In atherosclerotic animals we can observe a higher mesenteric opacity, increase in blood viscosity and a thickness in vessel wall. Thrombosis time is shortened at 3 days, which indicates a thrombogenic situation although at 10 days there is a lenthening in this parameter. Blood cell counts were not modified significantly, but modifications in differential leukocyte counts were significant. We found a direct relationship between lymphocyte number and thrombosis time whereas with granulocytes this relationship was inverse: shortening in thrombosis time appearing simultaneously with an increase in cell number.
Departamento de Farmacología y Patología Experimental, Centro de Investigación y Desarrollo de Barcelona, C.S.I.C., Spain.
C
C
Santolaya
M R
MR
Hernández
C A
CA
Villaverde
D000818:Animals; D001161:Arteriosclerosis / Q000097:blood / Q000209:etiology* / Q000473:pathology; D001772:Blood Cell Count; D003327:Coronary Disease / Q000150:complications*; D003328:Coronary Thrombosis / Q000150:complications*; D004195:Disease Models, Animal; D005260:Female; D008638:Mesenteric Arteries / Q000473:pathology; D009974:Orthomolecular Therapy; D051381:Rats; D011919:Rats, Inbred Strains; D012212:Rheology