Genetic conversion of G.C base-pairs to A.U base-pairs in a transfer RNA.
The cloverleaf stem segments of the suppressor gene of bacteriophage T4 tRNA(Gln) contain ten G.C and ten A.U base-pairs. To gain a better appreciation of the G.C base-pair requirement, we isolated multiple mutants of this suppressor gene in which base-pairs of G.C were replaced by A.U. One active suppressor gene contained only A.U base-pairs on the anticodon stem, indicating that G.C base-pairs in this region of tRNA(Gln) are not essential for function. In contrast, replacement was not possible at two base-pairs on the D stem and at one base-pair on the T stem.
Department of Bacteriology, University of Wisconsin, Madison 53706.
W H
WH
McClain
J G
JG
Seidman
D001482:Base Composition; D001483:Base Sequence; D005785:Gene Conversion; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012351:RNA, Transfer, Gln / Q000235:genetics*; D012367:RNA, Viral; D013489:Suppression, Genetic; D013604:T-Phages / Q000235:genetics
D012345:RNA, Transfer, Amino Acid-Specific; D012351:RNA, Transfer, Gln; D012367:RNA, Viral