Identification of a peanut agglutinin-binding protein from human retina.
With the use of an antiserum raised to peanut agglutinin (PNA)-binding protein(s) from human retinas, we have identified a glycoprotein that has the same subunit molecular weight (135,000) as human interphotoreceptor retinol-binding protein (IRBP) on human retinal protein transblots, and, like IRBP, is localized in the interphotoreceptor matrix surrounding both rods and cones. We show that this PNA-binding protein is not IRBP by comparing the binding patterns of antiserum to the PNA-binding protein to binding patterns obtained with antisera to bovine and monkey IRBP.
Jules Stein Eye Institute, UCLA School of Medicine 90024.
T A
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Shuster
A E
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Walter
D S
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Williams
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Farber
D010367:Arachis; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D037102:Lectins; D008970:Molecular Weight; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis*; D012160:Retina / Q000032:analysis*; D012177:Retinol-Binding Proteins / Q000032:analysis; D000099214:Retinol-Binding Proteins, Interstitial
D005136:Eye Proteins; D006023:Glycoproteins; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011975:Receptors, Mitogen; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial; C038223:peanut agglutinin receptor