Evidence for the co-storage and co-release of neuropeptide Y and noradrenaline from large dense cored vesicles in sympathetic nerves of the bovine vas deferens.
The subcellular localization and the secretion of neuropeptide Y were studied in sympathetic nerve endings of bovine vas deferens. Immunostaining revealed a parallel distribution for neuropeptide Y and dopamine-beta-hydroxylase immunoreactivity in the network of varicose nerve fibers in the smooth muscle layers of the vas deferens. Following differential centrifugation and sucrose density gradient centrifugation, neuropeptide Y was found to coincide with noradrenaline in the more dense region of the gradient, where the large dense cored vesicles are found. Superfusion experiments demonstrated the release of neuropeptide Y and noradrenaline upon electrical stimulation. Furthermore, the neuropeptide Y secretion was shown to be Ca2+-dependent. We conclude that in the bovine vas deferens neuropeptide Y is only present in large dense cored vesicles of adrenergic neurons and that the peptide and noradrenaline are co-released from these vesicles in a calcium dependent manner.
Department of Medicine, University of Antwerp, Wilrijk, Belgium.
W P
WP
De Potter
L
L
Dillen
W
W
Annaert
K
K
Tombeur
R
R
Berghmans
E P
EP
Coen
D000320:Adrenergic Fibers / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002417:Cattle; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004558:Electric Stimulation; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D009411:Nerve Endings / Q000378:metabolism / Q000648:ultrastructure; D009478:Neuropeptide Y / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D014649:Vas Deferens / Q000294:innervation* / Q000378:metabolism
D009478:Neuropeptide Y; D009638:Norepinephrine