Linkage between energy status of perivascular cells and mineralization of the chick growth cartilage.
The objective of this investigation was to investigate the relationship between the energy status of epiphyseal chondrocytes of the chick growth cartilage and the development of mineralization. A microfluorimetric scanning technique was used to measure the reduced pyridine nucleotide content of transverse sections of freeze-trapped cartilage; these measurements were related to tissue structure by scanning electron microscopy. The results of this study show that the energy status of cells in the hypertrophic region of the growth cartilage is more complex than was previously believed. In hypertrophic cartilage, most chondrocytes are in a reduced state. However, in the early hypertrophic region, the vascular channels that penetrate the cartilage from the metaphysis exert a profound local effect on the energy metabolism of perivascular chondrocytes. Thus, around each of the channels, there exists a zone of chondrocytes about 40-60 micron wide which exhibits a low fluorescence yield. The fluorescence level suggests that these perivascular cells have a higher level of oxidative metabolism than hypertrophic chondrocytes that are a distance (greater than 150 micron) from the vascular channels. This finding, in conjunction with our earlier observation that mineralization is first seen in the perivascular region, leads us to the conclusion that mineralization is associated with cellular oxidative activity. We now reject the long-held concept that in cartilage the development of mineralization is entirely due to tissue hypoxia.
Shapiro
I M
IM
Department of Biochemistry, University of Pennsylvania, Philadelphia 19104-6003.
Golub
E E
EE
Chance
B
B
Piddington
C
C
Oshima
O
O
Tuncay
O C
OC
Frasca
P
P
Haselgrove
J C
JC
D000818:Animals; D002356:Cartilage / Q000254:growth & development* / Q000378:metabolism; D002645:Chickens; D004734:Energy Metabolism; D005470:Fluorometry; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000378:metabolism*
D008903:Minerals