An in vitro model to study bacterial invasion of periodontal tissues.
In periodontal disease, the abilities of bacteria to adhere to and degrade in vivo basement membranes should be considered as two of the rate-limiting steps for the potential active or passive invasion of gingival connective tissues. To study these mechanisms in greater detail, we used the PF HR-9 basement-membrane-like matrix to establish an in vitro model of bacterial invasion and degradation. Three gram-negative anaerobic periodontopathic organisms, Bacteroides gingivalis, Fusobacterium nucleatum, and Actinobacillus actinomycetemcomitans, bound in considerably higher numbers to the HR-9 matrix than did 6 strains of gram-positive facultative organisms typically associated with periodontal health. In a further experiment with B. gingivalis, the organism rapidly degraded Type IV collagen, the major macromolecular component constituting the HR-9 matrix. Streptococcus mitis, the nonperiodontopathic bacterium tested, did not degrade this model matrix. This study provides evidence that B. gingivalis, a periodontopathic bacterium, is able to adhere to and degrade basement membranes, whereas nonperiodontopathic organisms appear not to share in these abilities.
Department of Stomatology, School of Dentistry, University of California, San Francisco 94143-0515.
J R
JR
Winkler
V
V
Matarese
C I
CI
Hoover
R H
RH
Kramer
P A
PA
Murray
D000188:Actinobacillus / Q000378:metabolism / Q000502:physiology; D001422:Bacterial Adhesion; D018407:Bacterial Physiological Phenomena; D001439:Bacteroides / Q000378:metabolism / Q000502:physiology; D001485:Basement Membrane / Q000378:metabolism; D046508:Culture Techniques; D005673:Fusobacterium / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D010510:Periodontal Diseases / Q000382:microbiology*; D010519:Periodontium / Q000378:metabolism / Q000382:microbiology*; D013291:Streptococcus / Q000378:metabolism / Q000502:physiology