Occurrence of ditaurobilirubin, bilirubin conjugated with two moles of taurine, in the gallbladder bile of yellowtail, Seriola quinqueradiata.
An unknown bilirubin conjugate was detected by HPLC analysis in the bile of yellowtail, Seriola quinqueradiata. The retention time of this unknown conjugate was identical with that of authentic ditaurobilirubin, a bilirubin conjugated with 2 mol of taurine. The retention times of the ethyl anthranilate diazo derivatives of both bilirubin conjugates were the same. The azo derivatives of this unknown conjugate were purified by Amberlite XAD-2 treatment and preparative TLC and hydrolyzed with 6 N HCl at 105 degrees C for 12 h. The hydrolyzates obtained were analyzed with an amino acid analyzer. The ninhydrin-positive substance in the hydrolyzates was purified by column chromatography and analyzed by TLC and mass spectrometry. The results of these analyses indicated that this ninhydrin-positive substance was taurine. Therefore, this unknown bilirubin conjugate was identified as ditaurobilirubin.
Department of Fisheries, Faculty of Agriculture, Miyazaki University.
T
T
Sakai
K
K
Watanabe
H
H
Kawatsu
D000818:Animals; D001391:Azo Compounds / Q000032:analysis / Q000302:isolation & purification; D001646:Bile / Q000032:analysis*; D001663:Bilirubin / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005399:Fishes / Q000378:metabolism*; D005704:Gallbladder / Q000378:metabolism; D007202:Indicators and Reagents; D013654:Taurine / Q000031:analogs & derivatives* / Q000032:analysis
D001391:Azo Compounds; D007202:Indicators and Reagents; D013654:Taurine; C046673:ditaurobilirubin; D001663:Bilirubin