Glycosylated chicken growth hormone.
In an attempt to raise monoclonal antibodies to chicken pituitary glycoprotein hormones, mice were immunized with the concanavalin A-adsorbed components of a hypophyseal extract. Fusions of these spleen cells with myeloma cells repeatedly yielded hybridoma lines secreting antibodies that recognized specifically the pituitary caudal acidophils, known as the somatotropes. This paper reveals the existence of a glycosylated counterpart of the well-established holoprotein form of chicken growth hormone, similar to what has been established for human growth hormone and prolactin.
Laboratory for Neuroendocrinology and Immunological Biotechnology, Louvain, Belgium.
L R
LR
Berghman
P
P
Lens
E
E
Decuypere
E R
ER
Kühn
F
F
Vandesande
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D013006:Growth Hormone / Q000032:analysis*
D013006:Growth Hormone