Determination of serum cholesterol on the Reflotron (BBR) dry chemistry analyser. Assessment of accuracy.
This investigation is aimed at assessing the accuracy of S-cholesterol determination by means of the Reflotron, an analytical system based upon the dry chemistry approach. Two sets of sera, one of them including specimens with elevated concentrations of urea, creatinine, glucose, urate or bilirubin, were assayed in parallel with the Refloton system, with a class-A "reference" method (including alkaline hydrolysis and extraction) and with a routine liquid chemistry enzymatic method. Results for both sets of sera indicated good agreement of the tested system with the routine method, whilst a small proportional negative bias was observed in comparison with the "reference" method. The use of the reference method as a basis for comparison allows more conclusive statements about accuracy to be made.
Laboratorio Analisis, H.S. Raffaele, Milano, Italy.
L C
LC
Sanguini
F
F
Ceriotti
C
C
Franzini
L
L
Grossi
P A
PA
Bonini
D001322:Autoanalysis / Q000592:standards*; D002784:Cholesterol / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D011786:Quality Control
D002784:Cholesterol