Countercurrent centrifugal elutriation in a table-top centrifuge.
An elutriation system has been developed which fits into a small table-top centrifuge. The new CS1 rotor functioned as accurately as the JE-6 rotor, commercially available from Beckman Instruments. However, fewer cells were required for the separation experiments. Leukocytes obtained from 25 ml whole blood by Ficoll-Hypaque gradient centrifugation were sufficient for separating lymphocytes from monocytes and for purifying granulocytes. Furthermore, when lymphocytes were harvested from cell cultures 40 ml of a suspension of 1.2 x 10(6) cells/ml were enough to yield an enriched preparation of antibody producing cells.
Bauer
J
J
Max-Planck-Institut für Biochemie, Martinsried, F.R.G.
Hannig
K
K
D000921:Antibody-Producing Cells / Q000166:cytology; D001773:Blood Cells / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002498:Centrifugation / Q000295:instrumentation; D003377:Countercurrent Distribution; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D009000:Monocytes / Q000166:cytology