A stopped-flow calorimeter for biochemical applications.
A rapid-response stopped-flow calorimeter for small samples of reagents is described. The construction, performance characteristics and operational limitations are described, along with an example of its ability to resolve the kinetics of an enzyme-catalysed hydrolysis. It is thought likely that the method would find useful application in a variety of chemical and biochemical investigations.
Marine Biological Association, Plymouth, U.K.
J V
JV
Howarth
N C
NC
Millar
H
H
Gutfreund
D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D002918:Chymotrypsin / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics
D002918:Chymotrypsin