Vaccination of cyclophosphamide-treated chickens against Newcastle disease virus infection.
Newly-hatched chickens were treated with 3 mg of cyclophosphamide (CY) per day for 4 consecutive days. At 2 weeks of age, these chickens, together with a group of untreated controls, were vaccinated intranasally or subcutaneously with the La Sota strain of Newcastle disease virus (NDV). All chickens were challenged intranasally with the GB strain of NDV 2 weeks later. CY-treated, intranasally vaccinated chickens were highly resistant to NDV challenge, yet none of the chickens produced any detectable humoral antibodies to NDV; antibodies to NDV were detectable in the tracheal washings, however.
Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
K M
KM
Lam
Q
Q
Hao
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002645:Chickens; D003520:Cyclophosphamide / Q000494:pharmacology*; D007136:Immunoglobulins / Q000032:analysis; D008297:Male; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology*; D014132:Trachea / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines
D000914:Antibodies, Viral; D007136:Immunoglobulins; D014765:Viral Vaccines; D003520:Cyclophosphamide