Stability of cytogenetic alterations in a human melanoma cell line and five clonal derivatives.
A cytogenetic study was done on a human malignant melanoma cell line and its 5 clones. Chromosome banding analysis indicated the presence of 7 "shared" markers (M) and 9 unique markers (m) that were present only in the clones. Chromosomes 1, 5, 9, 12, 17 and 21 were involved in M-markers and chromosomes 1, 2, 4, 6, 8, 9, 11, 16, 17, 18 and 21 were involved in m-marker formation. Both parental and clonal lines had near-triploid chromosome numbers. A number of M-markers were isochromosomes of the short (p) and long (q) arms of chromosome 1. Our cytogenetic data indicate that the parental line contained subpopulations of cells that were in different stages of karyotypic evolution.
Department of Cell Biology, University of Texas System Cancer Center M.D. Anderson Hospital and Tumor Institute, Houston 77030.
E J
EJ
de Lucca
S
S
Pathak
M C
MC
Cheung
D002460:Cell Line; D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000648:ultrastructure; D002999:Clone Cells / Q000473:pathology / Q000648:ultrastructure*; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008545:Melanoma / Q000235:genetics* / Q000648:ultrastructure
D005819:Genetic Markers