In vitro and in vivo effects of dichlorvos on blood cholinesterase activities of cattle.
Studies were conducted to ascertain in vitro effects and effects of percutaneous application (in vivo) of dichlorvos (2,2-dichlorovinyl dimethyl phosphate; DDVP) on cholinesterase activities in bovine erythrocytes and plasma. Treatment in vitro of erythrocytes and plasma with DDVP resulted in concentration- and time-dependent inhibition of erythrocyte acetylcholinesterase (AChE) and plasma cholinesterase (ChE) activities. Mean (+/- SD) DDVP concentrations required to cause 50% enzyme inhibition were 15.7 +/- 3.3 microM and 43.1 +/- 5.7 microM for AChE and ChE, respectively; however, these values required to achieve this inhibition were markedly decreased with increasing incubation time. The inhibited AChE activity failed to be reactivated after incubation of erythrocytes with 2-pyridine aldoxime methiodide (2-PAM); however, limited reactivation of inhibited AChE and ChE activities was observed with excess concentration of 2-PAM. Percutaneous application of a DDVP-containing livestock spray on cattle also caused a marked decrease in the in vivo activities of AChE and ChE; however, the inhibited enzyme activities were reactivated rapidly after incubation with 2-PAM.
Animal Sciences Wing, Alberta Environmental Centre, Vegreville, Canada.
A A
AA
Khan
R W
RW
Coppock
M M
MM
Schuler
L E
LE
Lillie
D000110:Acetylcholinesterase / Q000097:blood*; D000818:Animals; D002417:Cattle / Q000097:blood*; D002800:Cholinesterase Inhibitors / Q000097:blood / Q000378:metabolism; D002802:Cholinesterases / Q000097:blood*; D004006:Dichlorvos / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology; D008297:Male; D011220:Pralidoxime Compounds / Q000494:pharmacology; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D011220:Pralidoxime Compounds; D004006:Dichlorvos; D000110:Acetylcholinesterase; D002802:Cholinesterases; C028797:pralidoxime