Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins.
A mixture of the pyridylamino (PA) derivatives of 12 oligomannose-type sugar chains was fractionated into five fractions (mannose5N-acetylglucosamine2-PA approximately mannose9N-acetylglucosamine2-PA) by size-fractionation HPLC with a MicroPak AX-5 column. Each fraction thus obtained was then analyzed by reversed-phase HPLC with a Cosmosil 5C18-P column. In this way, the 12 PA-oligomannose-type sugar chains were completely separated from each other. The method was used to identify the structures of oligomannose-type sugar chains of human C3, the third component of human complement.
Department of Chemistry, Osaka University College of Science, Japan.
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Hase
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Natsuka
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Oku
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Ikenaka
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D003176:Complement C3 / Q000302:isolation & purification; D006023:Glycoproteins / Q000302:isolation & purification*; D006027:Glycosides / Q000302:isolation & purification*; D006801:Humans; D008362:Mannosides / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*
D003176:Complement C3; D006023:Glycoproteins; D006027:Glycosides; D008362:Mannosides; D009844:Oligosaccharides