9-Fluorenylmethoxycarbonylpyroglutamate, a side-product of derivatization of glutamate with 9-fluorenylmethyl chloroformate: a warning.
9-Fluorenylmethyl chloroformate derivatization is widely used for determinations of amino acids in biological material. The derivatization of glutamate leads to the formation of 9-fluorenylmethoxycarbonylpyroglutamate as a side-product, which is thus a methodological artifact.
Keller
H J
HJ
Institut für Hirnforschung, Universität Zürich, Switzerland.
Quang Do
K
K
Zollinger
M
M
Winterhalter
K H
KH
Cuénod
M
M
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes / Q000032:analysis*; D005971:Glutamates / Q000032:analysis*; D013058:Mass Spectrometry; D011760:Pyrrolidinones / Q000032:analysis*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D051381:Rats
D000596:Amino Acids; D005449:Fluorenes; D005971:Glutamates; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D011760:Pyrrolidinones; C054798:9-fluoroenylmethoxycarbonylpyroglutamate; C054007:1-(9-fluorenyl)methyl chloroformate; D011761:Pyrrolidonecarboxylic Acid