High-performance liquid chromatography analysis of 1-aminocyclopropane-1-carboxylic acid using o-phthaldialdehyde precolumn derivatization.
A simple, rapid, direct method for the HPLC analysis of 1-aminocyclopropane-1-carboxylic acid (ACC) as its o-phthaldialdehyde derivative is described. The method is sensitive to about 1 pmol and can be used on plant tissue extracts with no cleanup. It will prove valuable in plant extracts where the chemical conversion of ACC in the tissue extracts to ethylene is variable, or when analyzing the specific radioactivity of ACC produced from radiolabeled precursors.
Bushey
D F
DF
Section of Plant Biology, Cornell University, Ithaca, New York 14853.
Law
D M
DM
Davies
P J
PJ
D000596:Amino Acids / Q000032:analysis*; D000598:Amino Acids, Cyclic; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000032:analysis; D013050:Spectrometry, Fluorescence; D014908:Triticum / Q000032:analysis; D014675:Vegetables / Q000032:analysis; D009764:o-Phthalaldehyde
D000596:Amino Acids; D000598:Amino Acids, Cyclic; C023863:1-aminocyclopropane-1-carboxylic acid; D009764:o-Phthalaldehyde