An improved micromethod for infectivity assays and neutralization tests of dengue viruses.
An improved micromethod for infectivity assays and neutralization (N) tests of dengue (DEN) type 1-4 viruses was developed, using 96-well plates and the PAP (peroxidase-antiperoxidase) staining technique. The foci formed on BHK-21 cell monolayers in wells of the plate were readily countable under an ordinary stereomicroscope. This micromethod has the advantages over the micromethod of the Lab-Tek 8 chamber slide system of lower cost, requirement for smaller volumes of test sera and applicability to larger number of serum specimens for N tests of DEN viruses.
Ishimine
T
T
Department of Virology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Tadano
M
M
Fukunaga
T
T
Okuno
Y
Y
D000818:Animals; D002478:Cells, Cultured; D003716:Dengue Virus / Q000145:classification / Q000472:pathogenicity*; D051379:Mice; D009500:Neutralization Tests; D014774:Virulence