Multiple detection system for the high-resolution microcolumn size-exclusion chromatographic separation of proteins with a slurry-packed capillary column.
A multiple (triple) detection system with one detector and recorder each was developed for the high-resolution size-exclusion chromatographic separation of proteins with a slurry packed capillary column. Fused-silica capillary columns of length 1, 2.5 and 6 m and I.D. 0.26 mm were connected in this order by joining each end of the shorter column to the top of the longer column through flow-through cells installed side-by-side on a cell block in the detector. By this means it was possible to record the chromatograms three times with one injection. This system is convenient for comparing the chromatograms obtained with different plate numbers and for effecting very high-resolution separations.
Department of Applied Chemistry, Faculty of Engineering, Nagoya University, Japan.
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Hirose
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Ishii
D002850:Chromatography, Gel; D005634:Fructose-Bisphosphate Aldolase / Q000302:isolation & purification; D005696:Galactosidases / Q000302:isolation & purification; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D012116:Resins, Plant; D014510:Urease / Q000302:isolation & purification
D011506:Proteins; D012116:Resins, Plant; D005696:Galactosidases; D014510:Urease; D005634:Fructose-Bisphosphate Aldolase