Total sequential solid phase synthesis of rabbit skeletal troponin C calcium binding site III.
The total sequential solid phase synthesis of AcA98STnC(90-123)amide is described. The yield is comparable to that obtained previously using fragment condensation on the solid support. HPLC purification results in a product which displays greater calcium induced conformational changes than the peptide prepared by the fragment condensation method. However, the former has a dissociation constant for calcium (28 microM) seven times higher than the latter (4 microM).
Faculty of Pharmacy, University of Manitoba, Winnipeg, Canada.
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D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000138:chemical synthesis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D014336:Troponin / Q000138:chemical synthesis* / Q000378:metabolism; D019209:Troponin C
D002135:Calcium-Binding Proteins; D014336:Troponin; D019209:Troponin C; D002118:Calcium