Effect of naloxone on haemodynamic responses to acute blood loss in unanaesthetized rabbits.
1. We have tested the hypothesis that the pressor action of (-) naloxone HC1 after haemorrhage is due to antagonism of endogenous opiate mechanisms that are activated by haemorrhage, rather than to some more direct vasoconstrictor action of the drug. 2. Six conscious rabbits were treated intravenously with either naloxone (4 mg kg-1, then 0.1 mg kg-1 min-1) or equivalent volumes of saline. In unbled rabbits the naloxone regimen had no effect except to cause a transient bradycardia. After each treatment the rabbits were bled at a rate of 2.45 ml kg-1 min-1 until blood pressure fell to 40 mmHg or 28 ml kg-1 of blood had been withdrawn (17-24 ml kg-1 after saline, 21-28 ml kg-1 after naloxone). 3. Throughout both episodes of bleeding there was a progressive fall of cardiac output and rise of heart rate, at rates that were constant and independent of the prior treatment. 4. After saline treatment, bleeding at first resulted in a steep and progressive fall of systemic vascular conductance and a small fall in blood pressure. However, when blood loss exceeded 12.7 ml kg-1 (approximately 28% of blood volume) there was an abrupt rise in systemic vascular conductance and an abrupt fall in blood pressure. 5. After naloxone treatment, during the entire period of bleeding systemic vascular conductance fell steeply and blood pressure fell slowly. 6. The different effects of saline and naloxone on the haemodynamic responses to acute blood loss were not explicable by differences in haematocrit or net blood volume. 7. We conclude that endogenous opiate mechanisms are responsible for the abrupt vasodilation that occurs when more than 28% of blood volume is withdrawn rapidly from conscious rabbits. We suggest that these mechanisms reside in the central nervous system.
Baker Medical Research Institute, Melbourne, Australia.
J
J
Ludbrook
P C
PC
Rutter
D000208:Acute Disease; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001810:Blood Volume; D001835:Body Weight; D006339:Heart Rate / Q000187:drug effects; D006400:Hematocrit; D006439:Hemodynamics / Q000187:drug effects*; D006470:Hemorrhage / Q000503:physiopathology*; D009270:Naloxone / Q000494:pharmacology*; D011817:Rabbits; D012965:Sodium Chloride / Q000494:pharmacology; D014655:Vascular Resistance / Q000187:drug effects
D009270:Naloxone; D012965:Sodium Chloride