Histochemical detection of horseradish peroxidase activity in the rat by the vibratome-paraplast method.
A method is described for the histochemical detection of horseradish peroxidase in Paraplast Plus embedded brain sections. The procedure uses 150-micron-thick Vibratome-cut slices of glutaraldehyde-paraformaldehyde-fixed brain tissue. Tetramethylbenzidine stabilized by diaminobenzidine/cobalt/H2O2 is used as chromogen. The Vibratome-cut slices are dehydrated through a graded series of acetone, cleared in toluol and flat-embedded in Paraplast Plus embedding medium. Serial sections can be cut as thin as 5-7 micron. The method is universal in its application and permits optimal visualization of labeled neurons with great morphological detail at the light-microscopic level.
Orofacial Research Group, School of Dentistry, University of Groningen, The Netherlands.
R S
RS
Liem
J T
JT
Rokx
J D
JD
van Willigen
D000818:Animals; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014276:Trigeminal Nerve / Q000166:cytology / Q000201:enzymology
D006735:Horseradish Peroxidase; D010544:Peroxidases