The genes coding for human pro alpha 1(IV) collagen and pro alpha 2(IV) collagen are both located at the end of the long arm of chromosome 13.
We have isolated and characterized a cDNA clone containing DNA sequences coding for the noncollagenous carboxy-terminal domain of human pro alpha 2(IV) collagen. Using this cDNA clone in both Southern blot analysis of DNA isolated from human-mouse somatic-cell hybrids and in situ hybridization of normal human metaphase chromosomes, we have demonstrated that the gene coding for human pro alpha 2(IV) collagen is located at 13q33----34, in the same position on chromosome 13 as the pro alpha 1(IV) collagen gene.
Department of Surgery, UMDNJ-Robert Wood Johnson Medical School, New Brunswick 08903.
C D
CD
Boyd
S E
SE
Toth-Fejel
I K
IK
Gadi
M
M
Litt
M R
MR
Condon
M
M
Kolbe
I K
IK
Hagen
M
M
Kurkinen
J W
JW
Mackenzie
E
E
Magenis
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D003094:Collagen / Q000235:genetics*; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D051379:Mice; D009693:Nucleic Acid Hybridization; D011347:Procollagen / Q000235:genetics*
D005819:Genetic Markers; D011347:Procollagen; D003094:Collagen; D004247:DNA