The effect of glycolysis inhibitors on the recovery of CHO-K1 cells from split-dose irradiation.
The survival and recovery of CHO-K1 cells exposed to three inhibitors of different glycolytic enzymes was studied. All enzymes have previously been shown to reduce survival by acting on the survival curve shoulder. Split dose recovery, however, was increased when cells were treated with sodium iodoacetate or with sodium arsenate but decreased with sodium fluoride. The effect with the first two compounds was similar to that observed under similar conditions with glucose analogues, lactate, or oxamate and suggest that glycolytic energy metabolism is a factor in both survival and recovery of these cells from radiation.
Department of Radiobiology, Saint Luke's Hospital, Rathgar, Dublin, Ireland.
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Seymour
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Mothersill
D000818:Animals; D001149:Arsenates / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects*; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D006019:Glycolysis / Q000187:drug effects*; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D012969:Sodium Fluoride / Q000494:pharmacology; D014466:Ultraviolet Rays
D001149:Arsenates; D007461:Iodoacetates; C009277:sodium arsenate; D012969:Sodium Fluoride; D019807:Iodoacetic Acid