Evaluation of an enzyme-linked immunosorbent assay for the detection of Corynebacterium pseudotuberculosis infection in sheep.
Two enzyme-linked immunosorbent assays to measure antibody to the cell wall antigen (C-ELISA) and toxin antigen (T-ELISA) of C. pseudotuberculosis were evaluated on serum from 6 separate groups of sheep. For sheep naturally infected with C. pseudotuberculosis the sensitivity and specificity of the C-ELISA was 76% and 73% respectively and for the T-ELISA 67% and 77% respectively. For sheep slaughtered one year after artificial infection the sensitivity of both tests was greater than or equal to 83% and the specificity greater than or equal to 72%. For sheep slaughtered 4 months after artificial infection the specificity of both tests was less than 30% while the mean sensitivity was 85%. The C-ELISA in conjunction with the T-ELISA detected 92% of sheep with lung lesions.
Sutherland
S S
SS
Department of Agriculture, South Perth, Western Australia.
Ellis
T M
TM
Mercy
A R
AR
Paton
M
M
Middleton
H
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D003352:Corynebacterium / Q000276:immunology*; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008171:Lung Diseases / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008199:Lymphadenitis / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial