A variant gene and a pseudogene for human 5S RNA are transcriptionally active in vitro.
Screening of a human genomic DNA library with ribosomal 5S RNA yielded a variant 5S rRNA gene (pH5S1) and a pseudogene lacking the first 9 bp and the last 33 bp (pH5S2). Sequence analysis revealed that both genes contain several mutations in their coding region as compared to human 5S rRNA; however, their intragenic promoters are highly conserved. Both genes are transcribed in a homologous HeLa cell S100 extract. pH5S1 gives rise to a 5S-sized product, whereas the two pH5S2-derived RNAs are about 220 and 240 nucleotides long. pH5S1 is transcribed more efficiently than pH5S2; however, its ability to form a stable preinitiation complex is impaired.
Arnold
G J
GJ
Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Würzburg, F.R.G.
Kahnt
B
B
Herrenknecht
K
K
Gross
H J
HJ
D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D011544:Pseudogenes; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D014158:Transcription, Genetic
D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S