Chemical, physical, and biological characterization of a dimeric form of biosynthetic human growth hormone.
A dimer of biosynthetic human growth hormone (HGH) has been isolated and characterized. This entity, which is the predominant dimeric species in biosynthetic HGH, is chemically identical to monomeric HGH and exists in a noncovalent dimeric form which is dissociated to monomeric HGH on polyacrylamide electrophoresis gels or in aqueous solutions containing 30% acetonitrile. This substance, found in all production lots of pituitary HGH, biosynthetic HGH, and biosynthetic methionyl HGH examined, is much less biopotent than monomeric HGH and can be distinguished from monomeric HGH by a monoclonal antibody. These data demonstrate that polyacrylamide gel electrophoresis is not a valid method for measuring this dimer and that size-exclusion chromatography under aqueous conditions is required.
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
G W
GW
Becker
R R
RR
Bowsher
W C
WC
Mackellar
M L
ML
Poor
P M
PM
Tackitt
R M
RM
Riggin
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D001835:Body Weight / Q000187:drug effects; D001846:Bone Development / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005260:Female; D013006:Growth Hormone / Q000096:biosynthesis / Q000494:pharmacology; D006801:Humans; D007016:Hypophysectomy; D007118:Immunoassay; D046911:Macromolecular Substances; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D046911:Macromolecular Substances; D013006:Growth Hormone