[Effects of some physiologic factors on Bacillus sp. urease activity].
In this study, culture supernatant of Bacillus sp. has been used as crude enzyme source. It has been determined that 1% urea is the optimal substrate concentration for maximal urease activity. On the other hand, Bacillus sp. urease showed its maximal activity at pH:7.0-8.0, 30 degrees C. 50 minutes of incubation has been found to be the most suitable incubation period. The activity of the enzyme showed a 36% decrease when stored at +4 degrees C for a month.
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H. U. Fen Fakültesi Biyoloji Bölümü, Beytepe/Ankara.
D001407:Bacillus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism*
D014508:Urea; D014510:Urease
Bacillus sp. üreaz aktivitesine etkili bazi fizyolojik faktörler.