Nerve growth factor immunoreactivity in mouse kidney: an immunoelectron microscopic study.
We have recently reported the immunolocalization of nerve growth factor (NGF) in mouse kidney by light microscopy. In the present study, we have investigated the ultrastructural localization of NGF by the preembedding immunoperoxidase method for electron microscopy. NGF immunoreactivity was present in the connecting tubule cells of the distal nephron. These cells showed immunostaining associated with the Golgi complex, vesicles, rough endoplasmic reticulum (RER), and polyribosomes. The intercalated cells, in contrast, lacked immunoreactivity.
Department of Pathology, Harbor-UCLA Medical Center, Torrance 90509.
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Barajas
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Salido
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Laborde
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Fisher
D000818:Animals; D007150:Immunohistochemistry; D007684:Kidney Tubules / Q000032:analysis*; D007685:Kidney Tubules, Collecting / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009414:Nerve Growth Factors / Q000032:analysis*
D009414:Nerve Growth Factors