Protection of mice from fatal measles encephalitis by vaccination with vaccinia virus recombinants encoding either the hemagglutinin or the fusion protein.
Vaccinia virus recombinants encoding the hemagglutinin or fusion protein of measles virus have been constructed. Infection of cell cultures with the recombinants led to the synthesis of authentic measles proteins as judged by their electrophoretic mobility, recognition by antibodies, glycosylation, proteolytic cleavage, and presentation on the cell surface. Mice vaccinated with a single dose of the recombinant encoding the hemagglutinin protein developed antibodies capable of both inhibiting hemagglutination activity and neutralizing measles virus, whereas animals vaccinated with the recombinant encoding the fusion protein developed measles neutralizing antibodies. Mice vaccinated with either of the recombinants resisted a normally lethal intracerebral inoculation of a cell-associated measles virus subacute sclerosing panencephalitis strain.
Drillien
R
R
Transgène, Strasbourg, France.
Spehner
D
D
Kirn
A
A
Giraudon
P
P
Buckland
R
R
Wild
F
F
Lecocq
J P
JP
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000941:Antigens / Q000627:therapeutic use*; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004660:Encephalitis / Q000209:etiology / Q000517:prevention & control*; D006389:Hemagglutinins, Viral / Q000276:immunology / Q000627:therapeutic use*; D008457:Measles / Q000150:complications*; D008458:Measles Vaccine / Q000627:therapeutic use*; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000627:therapeutic use*; D014614:Vaccines, Synthetic / Q000627:therapeutic use*; D014616:Vaccinia virus / Q000235:genetics; D014760:Viral Fusion Proteins / Q000276:immunology / Q000627:therapeutic use*; D014764:Viral Proteins / Q000096:biosynthesis
D000914:Antibodies, Viral; D000941:Antigens; D006389:Hemagglutinins, Viral; D008458:Measles Vaccine; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D004247:DNA