Origin and evolution of a major feline satellite DNA.
A major satellite DNA has been cloned from the domestic cat (Felis catus) and characterized. The satellite monomer, termed FA-SAT, is 483 base-pairs in size, 64% G + C, and represents about 1 to 2% of the cat genome. A consensus sequence based upon partial sequence data from 21 independently isolated clones demonstrates: (1) FA-SAT is not composed of a series of shorter repeats, although about 25 copies, primarily imperfect, of the hexanucleotide TAACCC appear in the sequence; (2) there are many more CpG dinucleotides present in FA-SAT than expected for a random sequence of its size; and (3) 61% of all base substitutions in FA-SAT involve the replacement of G and C residues by A and T residues, indicating that FA-SAT is rapidly becoming A + T-rich. FA-SAT-related sequences are found in many mammals, where they appear to be scattered throughout the genome and not tandemly arranged as in the cat. An FA-SAT-related sequence was cloned from the domestic dog genome and sequenced, and shown to contain multiple copies of the same TAACCC hexanucleotide found in the cat satellite.
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
T G
TG
Fanning
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002415:Cats; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D004285:Dogs; D006168:Guinea Pigs; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite