Evidence that the functional beta-actin gene is single copy in most mice and is associated with 5' sequences capable of conferring serum- and cycloheximide-dependent regulation.
Hybridization to synthetic oligonucleotides representing conserved regions in the promoter and first intron of several vertebrate beta-actin genes was used to discriminate between what appears to be a single functional beta-actin gene and numerous pseudogenes in the mouse genome. Sequences derived from the 5' end of this gene were shown to confer serum-inducible expression upon a heterologous reporter gene when transfected into mouse fibroblasts. Moreover, these sequences rendered reporter gene expression superinducible by a combination of serum and cycloheximide. These experiments indicate that the 5' end of the mouse beta-actin gene contains sequence elements which mediate the stimulatory effects of serum growth factors and which are responsive to both positive and negative regulators of gene expression.
Elder
P K
PK
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905.
French
C L
CL
Subramaniam
M
M
Schmidt
L J
LJ
Getz
M J
MJ
D000123:Acetyltransferases / Q000235:genetics; D000199:Actins / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes; D006133:Growth Substances / Q000097:blood / Q000494:pharmacology*; D051379:Mice; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
D000199:Actins; D003470:Culture Media; D006133:Growth Substances; D011993:Recombinant Fusion Proteins; D003513:Cycloheximide; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase