Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria.
A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resorption has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E2. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide.
Hefley
T J
TJ
Krieger
N S
NS
Stern
P H
PH
D000818:Animals; D000831:Animals, Newborn; D001862:Bone Resorption / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis*; D046508:Culture Techniques; D015232:Dinoprostone; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D010010:Osteoclasts / Q000187:drug effects / Q000502:physiology; D010281:Parathyroid Hormone / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology
D010281:Parathyroid Hormone; D011458:Prostaglandins E; D003094:Collagen; D002117:Calcitriol; D015232:Dinoprostone