title: Involvement of prostaglandins in the response of frog adrenocortical cells to muscarinic receptor activation.
abstract: The role of prostaglandins (PGs) in the mechanism of action of acetylcholine (ACh) on frog adrenocortical cells has been examined. Administration of a single dose of ACh (5 X 10(-5) M) to perifused frog interrenal fragments, for 20 min, stimulated the production of corticosterone, aldosterone, PGE2 and 6-keto-PGF1 alpha. In contrast ACh did not significantly alter TXB2 production. The effect of ACh could be mimicked by muscarine (10(-5) M). Conversely, nicotine (10(-6) to 10(-4) M) was totally inactive. The increase in PG biosynthesis preceded the peak of corticosteroid release. Repeated 20-min pulses of ACh (5 X 10(-5) M) or muscarine (10(-5) M) given at 130-min intervals induced a desensitization phenomenon. In presence of indomethacin (5 X 10(-6) M), the effect of ACh on PG and steroid secretion was totally abolished. In calcium-free medium, the effect of ACh on PG and corticosteroid production was completely blocked. These results indicate that, in the frog, ACh stimulates corticosteroid secretion through a PG-dependent mechanism.
authors.forename: C
authors.initials: C
authors.lastname: Delarue
authors.forename: F
authors.initials: F
authors.lastname: Leboulenger
authors.forename: F
authors.initials: F
authors.lastname: Homo-Delarche
authors.forename: M
authors.initials: M
authors.lastname: Benyamina
authors.forename: I
authors.initials: I
authors.lastname: Lihrmann
authors.forename: I
authors.initials: I
authors.lastname: Perroteau
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000109:Acetylcholine / Q000494:pharmacology*; D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000450:Aldosterone / Q000096:biosynthesis*; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis*; D015232:Dinoprostone; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009116:Muscarine / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis*; D011895:Rana ridibunda; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*
chemical_list: D011458:Prostaglandins E; D011976:Receptors, Muscarinic; D000450:Aldosterone; D015121:6-Ketoprostaglandin F1 alpha; D009116:Muscarine; D015232:Dinoprostone; D000109:Acetylcholine; D003345:Corticosterone; D007213:Indomethacin