[Karyotypic characteristics of the murine myeloma line sp2/0-Ag14].
Using methods of G- and C-banding, a study was made of the karyotype of mouse myeloma cell line sp2/0-Ag14. The number of chromosomes varies from 58 to 65, the modal class being 61-62. 50 per cent of chromosomes are rearranged. Normal chromosomes 6, 12 and X were not detected in either examined cell of this line. Among the marker chromosomes there are an isochromosome (19/19), three dicentric markers and one marker with two interstitial C-bands. There is a specific marker t (12; 15) of mouse plasmacytomas in the karyotype sp2/0-Ag14. A possible association of specific translocations and segregations of the normal chromosomes with the phenotype of line sp2/0-Ag14 is discussed. The results obtained may be useful for cytogenetic analysis of hybridomas.
Sorokina
E A
EA
Kozhukharova
I V
IV
Grinchuk
T M
TM
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002871:Chromosome Banding; D005819:Genetic Markers; D007621:Karyotyping; D008677:Metaphase; D051379:Mice; D009101:Multiple Myeloma / Q000648:ultrastructure*
D005819:Genetic Markers
Kariotipicheskaia kharakteristika myshinoĭ mielomy linii sp2/0-Ag14.