Morphological alterations induced by patulin on cultured hepatoma cells.
Patulin is a mycotoxin produced by various Penicillium, Aspergillus and Byssochlamys species. To evaluate its inhibitory effect on cells, hepatoma tissue culture cells in suspension were incubated in presence of 30 microM of patulin for 7 h and investigated by transmission and scanning electron microscopy. By transmission electron microscopy, the most significant difference observed between treated and control cells was the disorganization of the cytoplasmic microfilaments in the treated cells. The disappearance of superficial membrane microvilli which contain microfibrillar material was visualized by scanning electron microscopy; the cells also presented protrusions. The effect of this toxin on the cytoskeleton can be compared to that exerted by colchicine or by cytochalasins.
Rihn
B
B
Lugnier
A A
AA
Dirheimer
G
G
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure; D008854:Microscopy, Electron; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D010365:Patulin / Q000633:toxicity*; D011714:Pyrans / Q000633:toxicity*; D051381:Rats
D011714:Pyrans; D010365:Patulin