Conditions influencing the induction of peroxisomal beta-oxidation in cultured rat hepatocytes.
Leibovitz L-15 medium was superior to RPM11640 in both maintenance and induction of peroxisomal beta-oxidation in cultured rat hepatocytes. Serum was not required for maintenance or induction of beta-oxidation. Cell plating density had only slight effects on maintenance and did not affect induction. Bezafibrate caused time- and dose-related increases in beta-oxidation, as well as increases in catalase, peroxisome volume fraction, and peroxisome number. This system maintained a greater percentage of control beta-oxidation than previously reported, and responded to several hypolipidemics with sizeable increases in activity. The flexibility of this system with respect to serum and cell-plating density facilitates simultaneous studies of cell parameters which are regulated by these variables.
Foxworthy
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Eacho
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D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology*; D002374:Catalase / Q000096:biosynthesis; D002470:Cell Survival / Q000187:drug effects; D002995:Clofibric Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D058607:Fibric Acids; D015248:Gemfibrozil; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000187:drug effects* / Q000378:metabolism; D010421:Pentanoic Acids / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344
D058607:Fibric Acids; D010421:Pentanoic Acids; D002995:Clofibric Acid; D002374:Catalase; C019304:ciprofibrate; D015248:Gemfibrozil; D001629:Bezafibrate