title: Homeostatic regulation of airway smooth muscle tone by catecholamine secretion in swine.
abstract: We studied the homeostatic secretory response of catecholamine secretion elicited by progressive bronchoconstriction in 18 swine in vivo. The potential reserve of the sympathetic nervous system (SNS) was first assessed by exogenous nicotinic stimulation with 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP). A dose of 250 micrograms/kg iv DMPP caused an increase in plasma norepinephrine (NE) concentration from 207 +/- 86 (basal) to 2,625 +/- 448 pg/ml (P less than 0.02) and in plasma epinephrine (EPI) from 10 +/- 5.0 to 1,410 +/- 432 pg/ml (P less than 0.05) in four swine. In four other swine, bronchoconstriction induced by aerosolized prostaglandin F2 alpha caused approximately a fivefold increase in airway resistance without hemodynamic changes. No increase in plasma EPI was observed. However, plasma NE increased from 330 +/- 131 to 1,540 +/- 182 pg/ml (P less than 0.02). In five swine receiving aerosolized acetylcholine (ACh), similar changes in airways resistance were not associated with significant changes in catecholamine concentration when mean arterial blood pressure (MAP) was unchanged. However, inhalation of sufficient ACh to cause a greater than 10% decrease in MAP caused progressive increase in catecholamine secretion. Plasma EPI increased from 32 +/- 16 (MAP = 124 +/- 7 Torr) to 1,165 +/- 522 pg/ml (MAP = 94 +/- Torr). Hypoxemia that occurred with bronchoconstriction (greater than or equal to 50 Torr) did not cause catecholamine secretion. However, severe hypoxemia (PO2 less than 30 Torr) caused large increases in plasma EPI concentrations from 84 +/- 27 to 1,463 +/- 945 pg/ml (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.forename: S R
authors.initials: SR
authors.lastname: White
authors.forename: M F
authors.initials: MF
authors.lastname: Sands
authors.forename: K J
authors.initials: KJ
authors.lastname: Popovich
authors.forename: T M
authors.initials: TM
authors.lastname: Murphy
authors.forename: N M
authors.initials: NM
authors.lastname: Munoz
authors.forename: J
authors.initials: J
authors.lastname: Blake
authors.forename: M
authors.initials: M
authors.lastname: Mack
authors.forename: T
authors.initials: T
authors.lastname: Shioya
authors.forename: A R
authors.initials: AR
authors.lastname: Leff
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D015237:Dinoprost; D004837:Epinephrine / Q000097:blood / Q000378:metabolism*; D005260:Female; D006706:Homeostasis; D000860:Hypoxia / Q000503:physiopathology; D007700:Kinetics; D008297:Male; D009130:Muscle, Smooth / Q000502:physiology*; D009638:Norepinephrine / Q000097:blood / Q000378:metabolism*; D011460:Prostaglandins F / Q000494:pharmacology; D012143:Respiratory Physiological Phenomena; D012137:Respiratory System / Q000187:drug effects; D013552:Swine; D013564:Sympathetic Nervous System / Q000502:physiology
chemical_list: D011460:Prostaglandins F; D015237:Dinoprost; D000109:Acetylcholine; D009638:Norepinephrine; D004837:Epinephrine