title: Regional localization of 18 human X-linked DNA sequences.
abstract: A series of human probes with unique sequences has been isolated from a recombinant phage library constructed with DNA obtained from a human-hamster hybrid cell line. This cell line contained the X chromosome as the only human component. For 18 of these probes, a human X-chromosome origin has been confirmed and they have been regionally assigned by a combination of techniques: dosage studies utilizing DNA from human fibroblasts carrying X-chromosome duplications and deletions; the presence or absence of hybridization to digested DNA from hybrid lines carrying fragments of the X chromosome; and in situ hybridization to metaphase chromosomes. The use of dosage as a means to regionally assign probes significantly improves resolution of the X chromosome.
authors.forename: D C
authors.initials: DC
authors.lastname: Riddell
authors.forename: H S
authors.initials: HS
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Beckett
authors.forename: A
authors.initials: A
authors.lastname: Chan
authors.forename: J J
authors.initials: JJ
authors.lastname: Holden
authors.forename: L M
authors.initials: LM
authors.lastname: Mulligan
authors.forename: M A
authors.initials: MA
authors.lastname: Phillips
authors.forename: N E
authors.initials: NE
authors.lastname: Simpson
authors.forename: K
authors.initials: K
authors.lastname: Wrogemann
authors.forename: J L
authors.initials: JL
authors.lastname: Hamerton
mesh_terms: D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D014960:X Chromosome
chemical_list: D005819:Genetic Markers; D004247:DNA