Antibody to a molecular marker of cell position inhibits synapse formation in retina.
A topographic gradient of TOP molecules in retina can be used to identify neuron position. Antibody to TOP from hybridoma cells that were injected into in vivo embryo eyes diffused into the retina and bound in a topographic gradient of [antibody.TOP] ([Ab.TOP]) complexes. Synapse formation in retina was inhibited in the presence of anti-TOP antibody. This suggests that TOP is involved in synapse formation and that recognition of position by neurons is necessary for normal synapse formation.
Trisler
D
D
Bekenstein
J
J
Daniels
M P
MP
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D002642:Chick Embryo; D005136:Eye Proteins / Q000276:immunology / Q000502:physiology*; D009024:Morphogenesis; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000196:embryology* / Q000276:immunology; D013569:Synapses / Q000502:physiology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D005136:Eye Proteins; D009419:Nerve Tissue Proteins