title: Procoagulant cellular activity (PCA) in the classification of acute leukemia.
abstract: The procoagulant cellular activity (PCA) of leukemic cells was evaluated, before and after endotoxin stimulation, in 38 patients with acute leukemia at presentation subdivided according to the FAB classification. In the M4 and M5 subgroups the stimulated leukemic cells showed a significant increase in the production of PCA compared with freshly isolated cells. No evident PCA was documented in M1 and M2 AML as well as in the majority of acute lymphoid leukemias tested, both before and after endotoxin stimulation. The myeloid and lymphoid leukemic cells appear to behave similarly to normal leucocytes, within which only monocyte/macrophages are capable of producing PCA following endotoxin stimulation. These findings suggest that in human leukemic cells the endotoxin-induced production of PCA may be considered a indicator of monocyte/macrophage differentiation and thus represent a valuable diagnostic tool in the classification of acute leukemias.
authors.forename: A
authors.initials: A
authors.lastname: Guarini
authors.forename: L
authors.initials: L
authors.lastname: Gugliotta
authors.forename: L
authors.initials: L
authors.lastname: Catani
authors.forename: L
authors.initials: L
authors.lastname: Chetti
authors.forename: S
authors.initials: S
authors.lastname: Macchi
authors.forename: S
authors.initials: S
authors.lastname: Tura
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D001779:Blood Coagulation Factors / Q000378:metabolism; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D006801:Humans; D007223:Infant; D007938:Leukemia / Q000145:classification* / Q000175:diagnosis; D007945:Leukemia, Lymphoid; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000254:growth & development / Q000378:metabolism
chemical_list: D001779:Blood Coagulation Factors; D004731:Endotoxins; C045071:leukocyte procoagulant activity