title: Isolation of pathogen-synthesized fraction that elicits phytoalexins in lucerne (Medicago sativa L.).
abstract: The production of phytoalexins by lucerne cultivars Dupuits (susceptible to Verticillium wilt) and Maris Kabul (resistant to Verticillum wilt) in response to substances (= elicitors) produced by pathogenic and non-pathogenic isolates of Verticillium albo-atrum was investigated. The elicitors are produced in culture filtrates from log-phase and past log-phase stages. The elicitors were isolated by gel-filtration and found to contain both protein and carbohydrate. They were found to be thermostable and non-dialyzable and proved to be sensitive to protease and sodium hydroxide treatment which suggests that they are glycoproteins. There was no evidence of specificity of the elicitors.
authors.affiliation: Department of Botany and Microbiology, University College of Swansea, Singleton Park, U.K.
authors.forename: O M
authors.initials: OM
authors.lastname: Onuorah
mesh_terms: D002478:Cells, Cultured; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D000455:Medicago sativa / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000032:analysis*; D010936:Plant Extracts / Q000302:isolation & purification*; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins
chemical_list: D006023:Glycoproteins; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins