The activity of human kidney arylsulfatases A and B towards catecholamine sulfates.
Both arylsulfatases (EC 3.1.6.1, ARS) A and B purified from human kidney displayed Michaelis-Menten kinetics towards catecholamines sulfates as substrates with Km values in the range of 4-25 mM. ARS A hydrolyzed adrenaline 3-sulfate and noradrenaline 3-sulfate with a maximal rate lower than that observed for cerebroside 3-sulfate. In contrast, ARS B hydrolyzed adrenaline 3-sulfate and noradrenaline 3-sulfate with a maximal rate similar to that observed for UDP-N-acetylgalactosamine 4-sulfate.
Demassieux
S
S
Romain
Y
Y
Bordeleau
L
L
Gravel
D
D
Carrière
S
S
D015239:Arylsulfotransferase; D002395:Catecholamines; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013379:Substrate Specificity; D013466:Sulfurtransferases / Q000378:metabolism*
D002395:Catecholamines; D013466:Sulfurtransferases; D015239:Arylsulfotransferase