Effect of lipid derivatives on invasion in vitro and on surface glycoproteins of three rodent cell types.
The antiinvasive activity on MO4 mouse cells of the following lipid derivatives was tested in vitro: an alkyl-lysophospholipid derivative (ET-18-OCH3), a thioether-phospholipid derivative (BM 41.440), an alkyl-linked lipoidal amine (CP-46,665) and a naturally occurring ester-linked phospholipid (2-LPC). In this test, BM 41.440 had the same antiinvasive potency as ET-18-OCH3, whereas CP-46,665 and 2-LPC had no effect on invasion. Comparison of the antiinvasive effect of ET-18-OCH3 on three types of cells showed the following ranking: 12R1C-RK rat kidney adenovirus type 12 transfected cells greater than MO4 mouse cells greater than LLC-H61 Lewis lung carcinoma cells. This ranking was not reflected in ET-18-OCH3-induced changes of cell surface exposed glycopeptides derived from the three types of cells metabolically labeled with radioactive fucose. The present and previous experiments suggested that changes in invasion caused by lipid derivatives depended upon relative cell surface fucosyl-glycopeptide alterations in both the invasive cells and the normal tissue.
Storme
G A
GA
Oncologic Center-Vrije Universiteit Brussel, Cancer Research Unit, Belgium.
Bruyneel
E A
EA
Schallier
D C
DC
Bolscher
J G
JG
Berdel
W E
WE
Mareel
M M
MM
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002460:Cell Line; D008246:Lysophospholipids / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D010755:Organophosphates / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010742:Phospholipid Ethers / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D008246:Lysophospholipids; D008562:Membrane Glycoproteins; D010755:Organophosphates; D009943:Organophosphorus Compounds; D010742:Phospholipid Ethers; D010880:Piperidines; C026659:edelfosine; C043297:ilmofosine; C038408:CP 46665