title: Expression of murine gamma fetal antigen in adult hematopoietic tissue and during induced differentiation of Friend erythroleukemia cells.
abstract: Quantitative analysis of extracts of various normal adult CD-1 mouse tissues indicated that the serologically defined murine gamma fetal antigen (gamma-FA) was expressed at high levels in hematopoietic tissue in general and in bone marrow (BM) in particular. Metabolic labeling of isolated BM cells indicated that the BM was a site of gamma-FA synthesis in the adult animal. The size(s) of the antigen immunoprecipitated from labeled BM cells (35 and 27 kilodaltons) with anti-gamma-FA serum correlated well with molecular weight estimates of fibrosarcoma-fetal mouse-associated gamma-FA, as determined by molecular sieve chromatography. For ascertainment of the relationship between hematopoietic cell differentiation and gamma-FA content, a multiparameter flow cytometric approach was used to evaluate gamma-FA levels in Friend erythroleukemia (FL) cells as a function of growth state (blast or dimethyl sulfoxide-differentiated) and cell-cycle compartment. Differentiated G1-arrested FL cells (G1D) possessed significantly lower gamma-FA-associated immunofluorescence as compared to control cells in the G0-G1 substate. Remaining S- and G2 + M-phase cells in differentiated populations demonstrated an even greater reduction in gamma-FA content relative to control cells in the corresponding cell-cycle phases. The available data support the tentative classification of gamma-FA as a murine differentiation antigen.
authors.forename: P J
authors.initials: PJ
authors.lastname: Higgins
authors.forename: A E
authors.initials: AE
authors.lastname: Silverstone
authors.forename: C
authors.initials: C
authors.lastname: Bueti
authors.forename: V F
authors.initials: VF
authors.lastname: Pizzi
authors.forename: M R
authors.initials: MR
authors.lastname: Melamed
authors.forename: M
authors.initials: M
authors.lastname: Lipkin
authors.forename: F
authors.initials: F
authors.lastname: Traganos
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D011232:Chemical Precipitation; D004247:DNA / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005326:Fetal Proteins / Q000032:analysis* / Q000276:immunology; D005622:Friend murine leukemia virus; D006413:Hematopoietic System / Q000276:immunology*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology* / Q000473:pathology; D007942:Leukemia, Experimental / Q000276:immunology*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D013462:Sulfur Radioisotopes
chemical_list: D000199:Actins; D000951:Antigens, Neoplasm; D005326:Fetal Proteins; D013462:Sulfur Radioisotopes; C021679:gamma-fetal antigen; D004247:DNA; D008715:Methionine; D004121:Dimethyl Sulfoxide