title: [Isolation and properties of carboxypeptidase from Streptomyces spheroides, strain 35].
abstract: Extracellular carboxypeptidase was isolated from culture filtrates of Str. spheroides strain 35, using affinity chromatography on bacitracin-silochrome, bacitracin-Sepharose and CABS-Sepharose. The electrophoretically homogenous enzyme was obtained with a 44% yield and 4160-fold purification. The enzyme-molecular weight is 33,000 Da; pI is 4.7. The amino acid composition of carboxypeptidase is as follows: Asp43, Thr30, Ser35, Glu33, Pro30, Gly47-50, Ala38, 1/2 Cys5-6, Val16, Met2, Ile11, Leu15, Tyr8, Phe10, Lys10, His6, Arg9. The enzyme shows an activity optimum at pH 7.5 is stable at pH 6-8, is completely inhibited with EDTA and can be reactivated by Ca2+. The carboxypeptidase from Str. spheroides strain 35 has a dual substrate specificity, i. e., it splits N-substituted di-, three- and tetrapeptides having both neutral and basic amino acids at the C-ends similar to mammalian carboxypeptidases A and B. The enzyme belongs to the family of metallocarboxypeptidases; its properties are very similar to those of carboxypeptidase S from Str. griseus K-1 and of carboxypeptidase T from Thermoactinomyces sp.
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaia
authors.forename: V G
authors.initials: VG
authors.lastname: Kreĭer
authors.forename: N S
authors.initials: NS
authors.lastname: Landau
authors.forename: N I
authors.initials: NI
authors.lastname: Tarasova
authors.forename: E A
authors.initials: EA
authors.lastname: Timokhina
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002268:Carboxypeptidases / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007700:Kinetics; D008970:Molecular Weight; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D002268:Carboxypeptidases; C055587:carboxypeptidase L
vernacular_title: Vydelenie i svoĭstva karboksipeptidazy Streptomyces spheroides, shtamm 35.