Pulse immunoassay for human immunoglobulin G using antibody bound latex beads.
Pulse immunoassay was applied in the immunoassay of human immunoglobulin G (H-IgG). Antibodies to H-IgG were covalently immobilized on the surface of latex beads (1 microM). Immunoreaction of the antibody bound latex beads (Ab-L) and H-IgG was performed using electric pulses. Agglutination rate (AR), defined by the following equation, was used to estimate the immunoreaction rate (formula; see text) where Nn is the total number of beads forming n bead agglutinations. Each Nn was determined by analyzing photographs of the samples. When 140 microliter of Ab-L suspension (0.7%) containing H-IgG (6.7 X 10(-6) g ml-1) was reacted using electric pulses (peak height 2 kV cm-1, pulse width 20 microseconds, pulse frequency 8 kHz), AR increased sharply and reached 50% after 10 min. In contrast, when the same sample was reacted without electric pulses, AR increased very slowly and only reached 20% after 20 min. Therefore, the immunoreaction of H-IgG and Ab-L can be carried out more rapidly by applying electric pulses.
Tamiya
E
E
Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.
Watanabe
N
N
Matsuoka
H
H
Karube
I
I
D000371:Agglutination; D000906:Antibodies; D000936:Antigen-Antibody Complex; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis*; D007700:Kinetics; D007840:Latex
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007840:Latex