title: Regulation of bacteriophage P2 late-gene expression: the ogr gene.
abstract: The ogr gene product of bacteriophage P2 is a positive regulatory factor required for P2 late-gene transcription. We have determined the nucleotide sequence of the ogr gene, which encodes a basic polypeptide of 72 amino acids. P2 growth is blocked by a host mutation, rpoA109, in the alpha subunit of DNA-dependent RNA polymerase. The ogr52 mutation, which allows P2 to grow in an rpoA109 strain, was shown to be a single nucleotide change, in the codon for residue 42, that changes tyrosine to cysteine. The predicted amino acid sequence of the Ogr protein does not show similarity to DNA-binding proteins that are known to affect promoter recognition, to sigma factors, or to other characterized transcriptional regulatory proteins. We have inserted the ogr gene into a plasmid under control of the leftward promoter and operator of bacteriophage lambda. Thermal induction of ogr gene expression in this plasmid results in overproduction of a small protein that has been shown by complementation to possess Ogr function.
authors.forename: G E
authors.initials: GE
authors.lastname: Christie
authors.forename: E
authors.initials: E
authors.lastname: Haggård-Ljungquist
authors.forename: R
authors.initials: R
authors.lastname: Feiwell
authors.forename: R
authors.initials: R
authors.lastname: Calendar
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014157:Transcription Factors; D014764:Viral Proteins