Growth and differentiation of cerebellar suspensions transplanted into the adult cerebellum of mice with heredodegenerative ataxia.
Cell suspensions from cerebellar primordia of 12-day mouse embryos were grafted into the cerebellum of 4-month-old Purkinje cell degeneration (pcd) mutant mice and examined 2-3 months later. In contrast to those of nontreated mutants, all of the grafted cerebella exhibited Purkinje cells that had migrated into the molecular layer, where they were clustered over its superficial two-thirds. These Purkinje cells develop flattened dendritic trees perpendicular to bundles of parallel fibers. Ultrastructural examination of their synaptic inputs and outputs disclosed that (i) as in normal cerebella, climbing fibers and axons from basket and stellate cells synapse on thick dendrites, whereas parallel fibers almost exclusively contact the distal spiny branchlets, and (ii) a substantial number of Purkinje cell axons reach their appropriate targets in the deep cerebellar nuclei, where they establish synaptic connections on large and small neurons. These results indicate that embryonic Purkinje cells grafted into the cerebellum of adult mice with heredodegenerative ataxia integrate themselves very specifically into the cerebellar circuitry of the recipient mouse, where they can replace the missing Purkinje cells. They also provide a morphological basis favoring the notion of functional restorative capabilities of neural grafts in systems in which neurons are connected in an almost point-to-point manner.
Sotelo
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Alvarado-Mallart
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D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002454:Cell Differentiation; D002465:Cell Movement; D002524:Cerebellar Ataxia / Q000235:genetics / Q000628:therapy*; D002531:Cerebellum / Q000196:embryology / Q000473:pathology / Q000637:transplantation; D003712:Dendrites / Q000648:ultrastructure; D006085:Graft Survival; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains / Q000235:genetics / Q000502:physiology; D011689:Purkinje Cells / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure; D013569:Synapses / Q000648:ultrastructure