Enhanced luminol-dependent chemiluminescence of stimulated rat alveolar macrophages by pretreatment with alveolar lining material.
Previous reports indicate that the in vitro bactericidal activity of rat alveolar macrophages (AM) is dependent on the lipid fraction (ALM-L) of the alveolar lining material (ALM). The present study demonstrates that luminol-dependent chemiluminescence of stimulated rat AM is increased when rat AM are preincubated in the ALM or in the ALM-L. Evidence is presented that oxidation of the unsaturated lipids is responsible for the increase. In addition to pretreatment with the ALM, cells were also pretreated in commercial preparations of several lipids found to be present in the ALM. Preincubation in these lipids produced a significant increase in the luminol-dependent chemiluminescence response. However, when a saturated lipid, dipalmitoyl phosphatidylcholine, was used no increase was found. Pretreatment in ALM did not increase the nitro blue tetrazolium dye reduction by the AM, nor was the phagocytosis of latex beads by the AM altered by the addition of the ALM.
Webb
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Jeska
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D000818:Animals; D008163:Luminescent Measurements; D008165:Luminol; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010587:Phagocytosis; D010743:Phospholipids / Q000502:physiology; D011650:Pulmonary Alveoli / Q000166:cytology / Q000502:physiology*; D011663:Pulmonary Surfactants / Q000502:physiology; D051381:Rats
D010743:Phospholipids; D011663:Pulmonary Surfactants; D009580:Nitroblue Tetrazolium; D008165:Luminol