Regulation of translocated c-myc genes transfected into plasmacytoma cells.
We have transfected two translocated c-myc oncogene clones, derived from two human lymphomas carrying the t(8;14) chromosome translocation, into mouse plasmacytoma cells to study the regulation of their expression. In one case, the transfected clone contained the two coding exons of the c-myc oncogene translocated to an immunoglobulin heavy-chain switch region; in the other case, the two coding exons were translocated 5' of the enhancer element located between the heavy-chain joining region (JH) and the switch region S mu. Nuclease S1 protection experiments indicate that only the c-myc translocated 5' of the enhancer element is transcribed in the plasmacytoma cells. Thus, 5'-truncation of the c-myc gene per se does not lead to c-myc deregulation. Further, since the level of c-myc transcripts in the parental human lymphoma cells was 3- to 4-fold higher than in the transfectants, it seems likely that additional elements within the heavy-chain locus may play a role in the enhancement of c-myc gene transcription in lymphoma cells.
Feo
S
S
Harvey
R
R
Showe
L
L
Croce
C M
CM
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D009857:Oncogenes; D010954:Plasmacytoma / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014178:Translocation, Genetic