[Radioimmunodetection of human lung cancer xenografts by isotope labeling monoclonal antibody ALT-04 in nude mice].
Three nude mouse models bearing the human lung cancer were established from two fresh surgical specimens and one cell line. Tumor histological structure and cell morphology were similar before and after transplantation. The monoclonal antibody ALT-04 (McAb ALT-04) against human lung cancer was labeled by 125I, 131I and 201Tl. Radioimmunodetection study showed that all the three kinds of xenografts in nude mice were specifically located by McAb ALT-04. Imaging examination confirmed the ability of isotope labeling McAb ALT-04 to detect the presence of transplanted human lung cancer tissues without the aid of background subtraction manipulations. It is suggested that McAb ALT-04 have the possibility of locating in the tumor diagnosis and guiding in the treatment.
Yu
Y B
YB
China-Japan Friendship Hospital, Beijing.
Ruan
S T
ST
Cai
L F
LF
Qiu
W R
WR
Fan
M Z
MZ
Chen
Z X
ZX
Wu
R H
RH
Ma
B C
BC
Wu
H
H
Zhong
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000912:Antibodies, Neoplasm / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging*; D002460:Cell Line; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D008175:Lung Neoplasms / Q000000981:diagnostic imaging*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011863:Radioimmunoassay; D011877:Radionuclide Imaging; D013794:Thallium Radioisotopes
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007457:Iodine Radioisotopes; D013794:Thallium Radioisotopes