A quantitative lymphocyte localization assay.
Multiple lesions (up to 100 sites) were induced in the skin of sheep using either allogeneic lymphocytes or, in BCG-sensitized animals, tuberculin. Cells recovered from an indwelling lymph catheter draining a prescapular lymph node were labeled with 111-indium, returned to venous blood, and allowed to circulate for 3 hours. Sheep were killed, and the skin lesions and lymph nodes were removed and counted in a gamma spectrometer. High levels of radioactivity (up to 38,000 cpm/lesion) were recovered from lesions, and only a few hundred cpm were recovered from comparable normal skin sites. Dose-response relationships and time kinetics were demonstrated for these lesions, and the radioactivity on blood and lymph cells was measured. The contribution of cell-free radioactivity was negligible. Using replicate injection sites, analytical, internally controlled studies can now be initiated to study the induction, promotion, and suppression of lymphocyte traffic.
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D000818:Animals; D005260:Female; D007204:Indium; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D011868:Radioisotopes; D012756:Sheep; D012867:Skin / Q000166:cytology / Q000473:pathology; D014373:Tuberculin / Q000276:immunology
D011868:Radioisotopes; D014373:Tuberculin; D007204:Indium