title: The effect of blood storage on lymphocyte subpopulations.
abstract: Duplicate heparinized blood samples were drawn from 22 subjects; 2 healthy subjects, 1 vitiligo and 19 with thyroid or parathyroid diseases. All 22 portion of specimens was immediately processed and examined for T cell subsets by indirect immunofluorescence, using monoclonal antibodies as control. 10 another portion of specimens were stored at 4 degrees C for 24 hours before testing. 12 another portion of specimens were pretreated with Ficoll-Hypaque separation before storage. When the whole blood was stored at 4 degrees C overnight and compared with samples tested with fresh cells, there was a significant decrease in OKT3+ cells (total T cells) (p less than 0.05) and a slight decrease in OKT4+ cells (T helper/inducer cells) and in OKT4/OKT8 ratio. However, these abnormalities were reversed when the lymphocytes were separated before storage. There was a significant decrease of natural killer cells (p less than 0.05) when specimens were pre-treated with Ficoll-Hypaque before storage. The reactivity of Ia+ cells was altered after overnight storage (p greater than 0.05).
authors.forename: H S
authors.initials: HS
authors.lastname: Huang
authors.forename: I J
authors.initials: IJ
authors.lastname: Su
authors.forename: M J
authors.initials: MJ
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D001793:Blood Preservation; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007694:Killer Cells, Natural; D008214:Lymphocytes / Q000145:classification*; D008297:Male; D008875:Middle Aged; D012034:Refrigeration
chemical_list: D000949:Histocompatibility Antigens Class II