title: Antineoplastic activity of the thioether lysophospholipid derivative BM 41.440 in vitro.
abstract: Thioether lysophospholipid derivatives (TLP) inhibited the in vitro uptake of [3H]thymidine into blasts of eight leukemias and cells of 12 different solid tumors of human origin. This effect correlated with trypan blue dye exclusion, which was used to assess cell damage. Cytostatic and cytotoxic effects of TLP were dependent on dosage and incubation time. Destruction of leukemic blasts was completed with greater than 5 micrograms/ml after an incubation of greater than 48 hr, but 10 to 20 micrograms/ml were necessary in solid tumors. Ester-linked 2-lysophosphatidylcholine was ineffective in the same dose range, which points to the requirement of the alkyl moiety in sn-1 and a stable sn-2 substitution of the molecule for the antineoplastic effect. To assess putative antileukemic selectivity, the cytotoxicity (trypan blue dye exclusion) of TLP was compared in human cell samples of 19 non-neoplastic bone marrows and 9 leukemias. Results revealed a significantly higher activity of the TLP BM 41.440 in leukemic blasts.
authors.affiliation: Department of Medicine I, Technische Universitaet, Munich, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Fromm
authors.forename: W E
authors.initials: WE
authors.lastname: Berdel
authors.forename: H D
authors.initials: HD
authors.lastname: Schick
authors.forename: U
authors.initials: U
authors.lastname: Fink
authors.forename: W
authors.initials: W
authors.lastname: Pahlke
authors.forename: U
authors.initials: U
authors.lastname: Bicker
authors.forename: A
authors.initials: A
authors.lastname: Reichert
authors.forename: J
authors.initials: J
authors.lastname: Rastetter
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007938:Leukemia; D008246:Lysophospholipids / Q000494:pharmacology*; D010755:Organophosphates / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010742:Phospholipid Ethers / Q000494:pharmacology; D013936:Thymidine; D014407:Tumor Cells, Cultured / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D008246:Lysophospholipids; D010755:Organophosphates; D009943:Organophosphorus Compounds; D010742:Phospholipid Ethers; C043297:ilmofosine; D013936:Thymidine