Brain microvascular smooth muscle expresses class II antigens.
Mouse (BALB/c) splenic lymphocytes co-cultured in vitro with syngeneic brain-derived microvascular smooth muscle (SM) proliferate and become activated. After subsequent transfer of the activated lymphocytes to a syngeneic host, a vasculitis develops in the host. Investigation of the possible antigen-presenting properties of the cultured SM has resulted in the demonstration of class II (Ia) antigens on the SM. Fluorescence-activated cell sorter analysis has shown that an average of 31% of unstimulated SM cells in culture were positive when stained with an anti-IE of the appropriate haplotype (H2d), and an average of 20% were positive with an anti-IA of the H2d haplotype. Controls consisting of irrelevant antibodies of the same isotype, as well as an anti-IA of the H2s haplotype, were negative. In contrast, BALB/c-derived brain microvascular endothelial cells showed considerably less class II antigen expression (7% for both IA and IE).
Hart
M N
MN
Waldschmidt
M M
MM
Hanley-Hyde
J M
JM
Moore
S A
SA
Kemp
J D
JD
Schelper
R L
RL
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000276:immunology*; D002453:Cell Cycle; D002478:Cells, Cultured; D004727:Endothelium / Q000276:immunology; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D051379:Mice; D008833:Microcirculation / Q000276:immunology*; D009131:Muscle, Smooth, Vascular / Q000276:immunology*
D000949:Histocompatibility Antigens Class II