A clinically useful ion-pairing high-performance liquid chromatographic assay for the monophosphate metabolites of thioguanine and mercaptopurine in human neoplastic cells.
A sensitive and rapid assay for the quantitation of thioinosine monophosphate and thioguanosine monophosphate, the major intracellular active metabolites of mercaptopurine and thioguanine, respectively, has been developed. Neoplastic cells are extracted with trichloracetic acid, and the neutralized acid extracts are analyzed by ion-pairing high-performance liquid chromatography with dual-channel uv-wavelength detection. This technique provides a lower limit of sensitivity of 30 pmol of thioinosine monophosphate and 10 pmol of thioguanosine monophosphate. The number of cells assayed per sample was 2 X 10(7). This assay makes it possible to detect and quantitate low levels of thioinosine monophosphate and thioguanosine monophosphate present in neoplastic cells obtained directly from patients receiving mercaptopurine or thioguanine chemotherapy.
Zimm
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Strong
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JM
D001853:Bone Marrow / Q000032:analysis; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006150:Guanine Nucleotides / Q000032:analysis* / Q000097:blood; D006801:Humans; D007291:Inosine Monophosphate / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood; D007292:Inosine Nucleotides / Q000032:analysis*; D015470:Leukemia, Myeloid, Acute; D008223:Lymphoma / Q000097:blood / Q000188:drug therapy; D015122:Mercaptopurine / Q000378:metabolism / Q000627:therapeutic use; D013866:Thioguanine / Q000378:metabolism / Q000627:therapeutic use; D013873:Thionucleotides / Q000032:analysis* / Q000097:blood
D006150:Guanine Nucleotides; D007292:Inosine Nucleotides; D013873:Thionucleotides; D007291:Inosine Monophosphate; C003964:6-thioguanylic acid; C003449:thioinosinic acid; D015122:Mercaptopurine; D013866:Thioguanine