title: Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.
abstract: We have examined the interaction of factors in HeLa cell nuclear extracts with a human histone H2B gene (H2B) promoter. Protein-DNA mobility-shift and DNase I protection assays detected a factor(s) binding to a 15-base-pair consensus element that is essential for efficient H2B transcription in vitro. Part of this consensus sequence is the octanucleotide ATTTGCAT, which is apparently a functional component of several non-histone genes. A subset of these genes, including a human U2 small nuclear RNA (snRNA) gene promoter, a mouse immunoglobulin heavy chain enhancer, and a mouse light chain promoter, were shown to interact with the H2B consensus sequence-binding factor(s). These results suggest that a common factor or closely related factors may contribute to the regulation of these and other genes that share the octanucleotide sequence.
authors.forename: H L
authors.initials: HL
authors.lastname: Sive
authors.forename: R G
authors.initials: RG
authors.lastname: Roeder
mesh_terms: D001483:Base Sequence; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D006367:HeLa Cells; D006657:Histones / Q000235:genetics*; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012342:RNA, Small Nuclear / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; D007136:Immunoglobulins; D012342:RNA, Small Nuclear; D014157:Transcription Factors