A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes.
1. A simple and rapid method for the reconstitution of Na+-dependent neutral amino acid transport activity from bovine renal brush border membranes is described. 2. The neutral detergent decanoyl-N-methylglucamide ('MEGA-10') was employed to solubilize the membrane protein. This obviated the necessity for a prolonged dialysis step. 3. The properties of amino acid transport in these vesicles were similar to those observed in native membranes. 4. This should be a useful procedure in the eventual identification and isolation of amino acid transport proteins.
Lynch
A M
AM
Department of Biochemistry, Medical School, Bristol, U.K.
McGivan
J D
JD
D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D005947:Glucose / Q000378:metabolism; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000378:metabolism; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010743:Phospholipids / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology*
D000596:Amino Acids; D008565:Membrane Proteins; D010743:Phospholipids; D012964:Sodium; D005947:Glucose; D000409:Alanine