New crystalline derivatives of bovine liver rhodanese.
Mitochondrial bovine liver rhodanese (thiosulfate:cyanide sulfurtransferase) has been crystallized in the form deprived of the transferable sulfur. The essential condition for crystallization was the removal of oxygen. Crystals of the sulfur-free enzyme are isomorphous with the previously characterized crystals of the sulfur-substituted enzyme. The new crystal species can react with either thiosulfate or selenosulfate to form the catalytic intermediate and, subsequently, with cyanide to form the corresponding product. Furthermore, the enzyme active site can be alkylated by iodoacetic acid.
Berni
R
R
Cannella
C
C
Monaco
H L
HL
Rossi
G L
GL
D000818:Animals; D002417:Cattle; D003460:Crystallization; D008930:Mitochondria, Liver / Q000201:enzymology*; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D013466:Sulfurtransferases / Q000302:isolation & purification*; D013884:Thiosulfate Sulfurtransferase / Q000302:isolation & purification* / Q000378:metabolism; D014961:X-Ray Diffraction
D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase