Synthesis and use of galactolipid sulfotransferase substrate-analogue affinity probes: possible localization of the testicular enzyme.
Galactosyl ceramide (GC) is a substrate in vitro for galactolipid sulfotransferase preparations from brain, kidney, and testis. Biotinylated and light-sensitive azido derivatives of lysogalactosyl ceramide have been synthesized. These derivatives remain effective substrates for the testicular enzyme. Biotinylated GC has been used to localize specific GC-binding sites in frozen sections of rat testis. The binding pattern is consistent with the expected location of the testicular galactolipid sulfotransferase.
Lingwood
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Taylor
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D000345:Affinity Labels / Q000138:chemical synthesis*; D000818:Animals; D001386:Azides / Q000138:chemical synthesis* / Q000378:metabolism; D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D002554:Cerebrosides / Q000138:chemical synthesis*; D005699:Galactosylceramides / Q000138:chemical synthesis* / Q000378:metabolism; D006651:Histocytochemistry; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000201:enzymology*
D000345:Affinity Labels; D001386:Azides; D002554:Cerebrosides; D005699:Galactosylceramides; C050345:azido-galactosylceramide; C050344:biotin-galactosylceramide; D001710:Biotin; D013466:Sulfurtransferases; D015238:Sulfotransferases; C020556:galactosylceramide sulfotransferase