A simple method for the quantitative evaluation of phagocytosis and intracellular killing by peripheral blood leukocytes.
Several immunodeficiency disorders result from phagocytic or intracellular killing defects. Evaluation is frequently impaired by the complexity of available tests. A new and simple method is presented for the evaluation of the phagocytic and intracellular killing activity by peripheral blood leukocytes, using a small blood volume. Normal individuals show a phagocytic index of 61.4 +/- 16.9% (range 44.5 - 78.3%) after 2 h and 73.1 +/- 13.0% (range 60.1 - 86.1%) after 3 h incubation. For the same incubation periods, the intracellular killing indices were 84.6 +/- 11.0% (range 73.6 - 95.6%) and 88.3 +/- 11.6%) (76.7 -99.9%), respectively.
Oliveira
E F
EF
Departamento de Patologia, Faculdade de Ciências Médicas, Universidade Estadual do Rio de Janeiro, Brasil.
Assis
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AB
Marques
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EA
D000165:Acridine Orange; D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology*; D008297:Male; D009504:Neutrophils / Q000502:physiology; D010587:Phagocytosis
D000165:Acridine Orange