Pulmonary surfactant and its components inhibit secretion of phosphatidylcholine from cultured rat alveolar type II cells.
Pulmonary surfactant is synthesized and secreted by alveolar type II cells. Radioactive phosphatidylcholine has been used as a marker for surfactant secretion. We report findings that suggest that surfactant inhibits secretion of 3H-labeled phosphatidylcholine by cultured rat type II cells. The lipid components and the surfactant protein group of Mr 26,000-36,000 (SP 26-36) inhibit secretion to different extents. Surfactant lipids do not completely inhibit release; in concentrations of 100 micrograms/ml, lipids inhibit stimulated secretion by 40%. SP 26-36 inhibits release with an EC50 of 0.1 microgram/ml. At concentrations of 1.0 microgram/ml, SP 26-36 inhibits basal secretion and reduces to basal levels secretion stimulated by terbutaline, phorbol 12-myristate 13-acetate, and the ionophore A23187. The inhibitory effect of SP 26-36 can be blocked by washing type II cells after adding SP 26-36, by heating the proteins to 100 degrees C for 10 min, by adding antiserum specific to SP 26-36, or by incubating cells in the presence of 0.2 mM EGTA. SP 26-36 isolated from canine and human sources also inhibits phosphatidylcholine release from rat type II cells. Neither type I collagen nor serum apolipoprotein A-1 inhibits secretion. These findings are compatible with the hypothesis that surfactant secretion is under feedback regulatory control.
Dobbs
L G
LG
Wright
J R
JR
Hawgood
S
S
Gonzalez
R
R
Venstrom
K
K
Nellenbogen
J
J
D000818:Animals; D001059:Apoproteins / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004285:Dogs; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008055:Lipids / Q000494:pharmacology; D008081:Liposomes / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011506:Proteins / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors
D001059:Apoproteins; D008055:Lipids; D008081:Liposomes; D010713:Phosphatidylcholines; D011506:Proteins; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; C046616:pulmonary surfactant apoprotein; D007770:L-Lactate Dehydrogenase