Expression of a 215,000-dalton nuclear envelope protein decreases during cell maturation.
The monoclonal antibody AGF2.3 identifies a nuclear envelope protein expressed at high levels in most transformed cell lines and weakly expressed in or absent from normal tissues and primary cell cultures. Here we report that expression of AGF2.3 antigen relates to the maturation status of cells. When HL60 cells were induced to mature loss of AGF2.3 antigen correlated with the appearance of neutrophils in culture. Acute myeloid leukaemia cells were intensely labelled by AGF2.3 antibody whereas chronic myeloid leukaemia cells were weakly reactive. Staining of tonsil epithelium showed a progressive loss of antigen during epithelial cell maturation. High levels of AGF2.3 antigen in most permanent cell lines suggest that such lines represent transformation of immature cells.
Brown
G
G
Bunce
C M
CM
Johnson
G D
GD
Lord
J M
JM
D000911:Antibodies, Monoclonal / Q000276:immunology; D001853:Bone Marrow / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D004848:Epithelium / Q000032:analysis; D006801:Humans; D007938:Leukemia / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D009504:Neutrophils / Q000032:analysis; D009685:Nuclear Envelope / Q000032:analysis*; D009698:Nucleoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D009698:Nucleoproteins