Complement component C3 secretion by mouse macrophage-like cell lines.
Secretion of complement component C3 by the mouse macrophage-like cell lines PU5-1.8, J774A.1, RAW264.7, and P388D1 was measured using an enzyme-linked immunosorbent assay for mouse C3. All cell lines secreted antigenically detectable C3 with the relative secreted C3/10(6) cells/24 h ranked as J774A.1 greater than P388D1 greater than or equal to PU5-1.8 much greater than RAW264.7. C3 secretion was enhanced two- to fourfold in cultures of all cell lines when treated with lipopolysaccharide, streptococcal cell walls, or lymphokine-containing supernatant fluids of mitogen-stimulated spleen cells. A differential induction of C3 synthesis and secretion was indicated since secreted lysozyme and total cellular protein were not elevated in a manner comparable to C3. The relative inducibility of cell lines for C3 secretion in either lipopolysaccharide- or cell wall-treated cells could be ranked as PU5-1.8 greater than P388D1 greater than J774A.1 greater than RAW264.7. C3 secretion was inhibited by cycloheximide or hydrocortisone. Mouse macrophage-like cell lines retain baseline and inducible C3 synthetic activities as do normal macrophages and can serve as homogeneous cultures in which to study regulation of complement biosynthesis.
Goodrum
K J
KJ
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D002460:Cell Line; D003167:Complement Activation; D003176:Complement C3 / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006854:Hydrocortisone / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D013268:Stimulation, Chemical; D013291:Streptococcus / Q000502:physiology
D001426:Bacterial Proteins; D003176:Complement C3; D008070:Lipopolysaccharides; D008222:Lymphokines; D003513:Cycloheximide; D006854:Hydrocortisone