[Cloned fragment of human alphoid DNA--a molecular marker of the pericentromeric region of chromosome 18].
From the library of cloned fragments of human DNA we have isolated two recombinant plasmids containing alphoid DNA sequences pBRHS13, pBRHS65. Both cloned sequences hybridized in situ predominantly to pericentromeric regions of chromosome 18 and with less intensity to pericentromeric regions of chromosomes 2, 9, 20, and were characterized by populational copy number polymorphism in homologous chromosomes. These sequences may appear very useful in the diagnostics and cytogenetic analysis of chromosomal aberrations and in studies of polymorphisms of heterochromatic regions of human chromosomes.
Aleksandrov
I A
IA
Iurov
Iu B
IuB
Mitkevich
S P
SP
Gindilis
V M
VM
D002503:Centromere / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D002902:Chromosomes, Human, 16-18 / Q000648:ultrastructure*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D005819:Genetic Markers; D006801:Humans; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D004274:DNA, Recombinant; D005819:Genetic Markers; D004247:DNA
Klonirovannyĭ fragment al'foidnoĭ DNK cheloveka--molekuliarnyĭ marker pritsentromernogo raĭona 18-ĭ khromosomy.