Prostaglandin-E2 9-ketoreductase from human uterine decidua vera.
Prostaglandin-E2 9-ketoreductase, the enzyme which catalyzes the reaction from prostaglandin E2 (PGE2) to prostaglandin F2 alpha (PGF2 alpha), has been purified 232-fold from human uterine decidua vera. The molecular mass of the enzyme, as estimated by fast protein liquid chromatography, was 29 kDa. Sodium dodecyl sulfate disc gel electrophoresis of the denatured enzyme revealed a molecular mass of 31 kDa. These data suggest that the enzyme consists of a single polypeptide chain. The rate equation of the enzyme reaction for two substrates was used for the determination of five kinetic constants. The equilibrium constant with respect to PGE2 was 83 microM, the Michaelis constant, Km, for PGE2 was 93 microM. For NADPH, the equilibrium constant was 1.0 microM and Km was 1.6 microM. The maximal velocity for the forward reaction was V1 = 217 pmol/min. The inhibition constants for the analgesic agents indomethacin and fentiazac were Ki = 850 microM and Ki = 450 microM and for the steroid progesterone Ki = 1.5 mM, respectively. Prostaglandin-E2 9-ketoreductase might be responsible for the control of the PGE2/PGF2 alpha ratio in human decidua vera. The enzyme, therefore, might be an important factor in the cascade of events leading to uterine contractions and parturition.
Krüger
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Schlegel
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D000085:Acetates / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003656:Decidua / Q000201:enzymology*; D015237:Dinoprost; D015232:Dinoprostone; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D008970:Molecular Weight; D009249:NADP / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology
D000085:Acetates; D011458:Prostaglandins E; D011460:Prostaglandins F; D013844:Thiazoles; C006124:fentiazac; D011374:Progesterone; D009249:NADP; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin