Introduction of estrogen-responsiveness into mammalian cell lines.
We introduced estrogen responsiveness as a new characteristic into rat hepatoma, mouse Ltk- and human HeLatk-cells by transfecting the human estrogen receptor (ER) cDNA. To measure the estrogen response we used Xenopus vitellogenin gene A2 constructs linked to the bacterial CAT gene. Transient cotransfections of the ER cDNA and the vitellogenin gene-CAT constructs containing the estrogen responsive element (ERE) lead to a hormone dependent induction of CAT activity whereas cotransfected vitellogenin gene constructs lacking the ERE are not inducible. Stable transfections of ER cDNA into Ltk- cells give rise to cell clones that are estrogen responsive as shown by transfection of various vitellogenin gene-CAT constructs. These results prove that the transfected ER is biologically active and is sufficient to make a cell estrogen responsive.
Druege
P M
PM
Klein-Hitpass
L
L
Green
S
S
Stack
G
G
Chambon
P
P
Ryffel
G U
GU
D000123:Acetyltransferases / Q000235:genetics; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D004247:DNA; D004967:Estrogens / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D010957:Plasmids; D011960:Receptors, Estrogen / Q000235:genetics*; D014162:Transfection; D014819:Vitellogenins / Q000235:genetics
D004967:Estrogens; D011960:Receptors, Estrogen; D014819:Vitellogenins; D004247:DNA; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase