title: Prostaglandin synthesis by rat uterine homogenates in the presence of copper ions, and the effects of indomethacin and salicylic acid.
abstract: While no significant effects on the in vitro production of PGF2 alpha by homogenates of rat estrous uteri were observed in the presence of 10(-3) and 10(-6) M Cu2+, the presence of Cu2+ at 10(-4) and 10(-5) M was found to stimulate production with maximal synthesis of PGF2 alpha occurring with 10(-4) M Cu2+. By contrast, the synthesis of PGE2 and PGI2 (determined as 6-keto PGF1 alpha) were unaffected at all of the different Cu2+ concentration used. When indomethacin and salicylic acid were tested for their effects on the Cu2+-mediated levels of PG synthesis by the homogenates, indomethacin (at 20 microM) was found to cause similar pronounced decreases in PGF2 alpha, PGE2 and 6-keto PGF1 alpha whereas salicylic acid (400 microM) showed preference towards suppressing PGE2 and 6-keto PGF1 alpha production.
authors.forename: P V
authors.initials: PV
authors.lastname: Peplow
authors.forename: P R
authors.initials: PR
authors.lastname: Hurst
mesh_terms: D000818:Animals; D003300:Copper / Q000494:pharmacology*; D015237:Dinoprost; D004971:Estrus; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D007700:Kinetics; D011453:Prostaglandins / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011453:Prostaglandins; D011460:Prostaglandins F; D012459:Salicylates; D003300:Copper; D015237:Dinoprost; D020156:Salicylic Acid; D007213:Indomethacin