Graphical and statistical analysis of hyperbolic tight-binding inhibition.
Equations are described for the analysis of enzyme-catalysed reactions in the presence of hyperbolic tight-binding inhibitors using both graphical and non-linear-regression methods. The general character of the equations allows the interpretation of several other kinetic mechanisms. As an example, the tight-binding hyperbolic non-competitive inhibition of human leucocyte elastase by a polysulphated glycosaminoglycan is analysed.
Baici
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Department of Rheumatology, University Hospital, Zurich, Switzerland.
D004791:Enzyme Inhibitors; D006025:Glycosaminoglycans / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology; D008954:Models, Biological; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000097:blood
D004791:Enzyme Inhibitors; D006025:Glycosaminoglycans; C013786:A73025; D010196:Pancreatic Elastase