title: 1-B-D arabinofuranosyl cytosine and all-trans retinoic acid in combination accelerates and increases monocyte differentiation of myeloid leukemic cells.
abstract: The effect of the combination of two different agents on myeloid differentiation was studied. Human promyelocytic cells (HL-60) and monoblast-like cells (U-937) were treated with 1-B-D arabinofuranosyl cytosine (Ara-C) alone, or in combination with retinoic acid (trans-RA). The known dual potentiality of HL-60 promyelocytes was confirmed by their ability to mature into granulocytes following induction by retinoic acid and into monocyte-like cells following treatment with 1-B-D arabinofuranosyl cytosine. The U-937 cell line differentiated to monocyte-like cells with either of the two drugs. The differentiation induced by Ara-C involved an irreversible step after 24-h incubation with the drug, was concentration dependent, and far more superior on U-937 cells than on HL-60 cells. The outcome of these two cell lines after treatment with both Ara-C and trans-RA was also different: this combination maintained the monocyte differentiation in the HL-60 cell line, and resulted in a higher sensitivity of U-937 cells to Ara-C, as indicated by a cell response to low Ara-C concentrations and a more rapid expression of monocyte specific properties.
authors.forename: C
authors.initials: C
authors.lastname: Chomienne
authors.forename: N
authors.initials: N
authors.lastname: Balitrand
authors.forename: L
authors.initials: L
authors.lastname: Degos
authors.forename: J P
authors.initials: JP
authors.lastname: Abita
mesh_terms: D000911:Antibodies, Monoclonal; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002478:Cells, Cultured; D003561:Cytarabine / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D007536:Isomerism; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D009580:Nitroblue Tetrazolium; D014212:Tretinoin / Q000008:administration & dosage*
chemical_list: D000911:Antibodies, Monoclonal; D003561:Cytarabine; D009580:Nitroblue Tetrazolium; D014212:Tretinoin