title: Polymer resins with amino acid containing pendants for sorption of bilirubin. III. Adsorption and desorption in the presence of albumin.
abstract: The adsorption of bilirubin from aqueous phosphate buffer solutions (pH = 7.8) containing bovine serum albumin (BSA) by polyacrylamide resins, with pendants consisting of the oligopeptides (Arg)5(Ala)3-, (Arg)2(Ala)3-, (Lys)5(Ala)3-, His(Arg)2(Ala)3-, has been studied at 0 degrees C under N2. The ability of the oligopeptide-resins to retain adsorbed bilirubin in the presence of BSA follows the order (Arg)5(Ala)3- greater than (Arg)2(Ala)3- greater than (Lys)5(Ala)3- = His(Arg)2(Ala)3-resin. The (Arg)5(Ala)3-resin is able to abstract the second bilirubin on the albumin assuming that BSA binds two bilirubin molecules strongly.
authors.affiliation: Department of Chemistry, McGill University, Montreal, Canada.
authors.forename: D S
authors.initials: DS
authors.lastname: Henning
authors.forename: S D
authors.initials: SD
authors.lastname: Clas
authors.forename: G R
authors.initials: GR
authors.lastname: Brown
authors.forename: L E
authors.initials: LE
authors.lastname: St-Pierre
mesh_terms: D000180:Acrylic Resins; D000327:Adsorption; D000818:Animals; D001663:Bilirubin / Q000097:blood / Q000302:isolation & purification; D002021:Buffers; D002417:Cattle; D003080:Cold Temperature; D009842:Oligopeptides; D011485:Protein Binding; D012710:Serum Albumin, Bovine
chemical_list: D000180:Acrylic Resins; D002021:Buffers; D009842:Oligopeptides; C016680:polyacrylamide gels; D012710:Serum Albumin, Bovine; D001663:Bilirubin