title: Myeloid and erythroid lineage expression of haemopoietic progenitors derived from an abnormal clone in erythroleukaemia.
abstract: To clarify the lineage involvement of haemopoietic progenitor cells in erythroleukaemia, the morphology and chromosomes of single colonies from a patient with erythroleukaemia were analysed simultaneously. The cytogenetic analysis of bone marrow cells revealed two clones; 44,XY,-7,-12,-17, del (5)(q31), +Mar and 43,XY,-7,-12,-17,-19,del(5)(q31),+Mar. Of 40 metaphases examined, there were 34 and six of these clones, respectively. Bone marrow mononuclear cells were plated at 5 X 10(4)/ml in methylcellulose medium containing phytohaemagglutinin-stimulated leucocyte conditioned medium and erythropoietin. Seventeen colonies, i.e. nine blast cell colonies, four myeloid (Sudan black B-positive) colonies, and four erythroid (benzidine-positive) colonies contained analysable metaphases, yielding 102 metaphases in total. Except for chromosome random loss, the karyotype within a colony remained constant. All three types of colonies showed an abnormal clone; 44,XY,-7,-12,-17,del(5)(q31),+Mar. From these findings, it is concluded that myeloid and erythroid lineages in erythroleukaemia were derived from the same abnormal clone.
authors.forename: T
authors.initials: T
authors.lastname: Suda
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Furukawa
authors.forename: J
authors.initials: J
authors.lastname: Suda
authors.forename: M
authors.initials: M
authors.lastname: Eguchi
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
mesh_terms: D000368:Aged; D001853:Bone Marrow / Q000648:ultrastructure; D002869:Chromosome Aberrations; D002999:Clone Cells / Q000648:ultrastructure; D006412:Hematopoietic Stem Cells / Q000648:ultrastructure*; D006801:Humans; D007621:Karyotyping; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron