title: A simple method for the quantitative evaluation of phagocytosis and intracellular killing by peripheral blood leukocytes.
abstract: Several immunodeficiency disorders result from phagocytic or intracellular killing defects. Evaluation is frequently impaired by the complexity of available tests. A new and simple method is presented for the evaluation of the phagocytic and intracellular killing activity by peripheral blood leukocytes, using a small blood volume. Normal individuals show a phagocytic index of 61.4 +/- 16.9% (range 44.5 - 78.3%) after 2 h and 73.1 +/- 13.0% (range 60.1 - 86.1%) after 3 h incubation. For the same incubation periods, the intracellular killing indices were 84.6 +/- 11.0% (range 73.6 - 95.6%) and 88.3 +/- 11.6%) (76.7 -99.9%), respectively.
authors.affiliation: Departamento de Patologia, Faculdade de Ciências Médicas, Universidade Estadual do Rio de Janeiro, Brasil.
authors.forename: E F
authors.initials: EF
authors.lastname: Oliveira
authors.forename: A B
authors.initials: AB
authors.lastname: Assis
authors.forename: E A
authors.initials: EA
authors.lastname: Marques
mesh_terms: D000165:Acridine Orange; D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology*; D008297:Male; D009504:Neutrophils / Q000502:physiology; D010587:Phagocytosis
chemical_list: D000165:Acridine Orange