Glutathione peroxidase protein. Absence in selenium deficiency states and correlation with enzymatic activity.
Glutathione peroxidase (GSHPx) activity is an indicator of selenium status in selenium-deficient individuals. Utilizing polyclonal monospecific antibodies to purified erythrocyte GSHPx, we were able to determine the relationship between enzymatic activity and protein content. In erythrocytes from a selenium-deficient individual who was treated with selenium, and in HL-60 cells grown in the absence of selenium and then returned to selenium-containing medium, there was a direct relationship between enzymatic activity and protein content. Thus, selenium deficiency results not only in a decrease of GSHPx activity, but also in a decrease of GSHPx protein.
Takahashi
K
K
Newburger
P E
PE
Cohen
H J
HJ
D000911:Antibodies, Monoclonal; D004912:Erythrocytes / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000032:analysis*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D012643:Selenium / Q000172:deficiency*
D000911:Antibodies, Monoclonal; D005979:Glutathione Peroxidase; D012643:Selenium