title: Encapsulation of thiosulfate: cyanide sulfurtransferase by mouse erythrocytes.
abstract: Murine carrier erythrocytes, prepared by hypotonic dialysis, were employed in the encapsulation of several compounds including [14C]sucrose, [3H]inulin, and bovine thiosulfate:cyanide sulfurtransferase (rhodanese), a mitochondrial enzyme which converts cyanide to thiocyanate. Approximately 30% of the added [14C]sucrose, [3H]inulin, and rhodanese was encapsulated by predialyzed erythrocytes, and a decrease in the mean corpuscular volume and mean corpuscular hemoglobin was observed. In the encapsulation of rhodanese a recovery of 95% of the erythrocytes was achieved and an 85% equilibrium was established. The addition of potassium cyanide (50 mM) to intact, rhodanese-loaded erythrocytes containing sodium thiosulfate resulted in its metabolism to thiocyanate. These results establish the potential use of erythrocytes as biodegradable drug carrier in drug antagonism.
authors.forename: P
authors.initials: P
authors.lastname: Leung
authors.forename: L E
authors.initials: LE
authors.lastname: Ray
authors.forename: C
authors.initials: C
authors.lastname: Sander
authors.forename: J L
authors.initials: JL
authors.lastname: Way
authors.forename: D M
authors.initials: DM
authors.lastname: Sylvester
authors.forename: J L
authors.initials: JL
authors.lastname: Way
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D003486:Cyanides / Q000378:metabolism*; D003956:Dialysis; D004912:Erythrocytes / Q000201:enzymology*; D008658:Inactivation, Metabolic; D007444:Inulin / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009994:Osmolar Concentration; D013395:Sucrose / Q000097:blood; D013466:Sulfurtransferases / Q000097:blood*; D013884:Thiosulfate Sulfurtransferase / Q000008:administration & dosage / Q000097:blood*; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D003486:Cyanides; D014316:Tritium; D013395:Sucrose; D007444:Inulin; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase