[A matrix and method of preparing 2-fragment specimens for ultramicrotomy].
A matrix for the preparation of two-fragment specimens for ultramicroscopy of biologic objects is described. The matrix has the form of a monolithic plate 8-10 mm thick made of a material such as fluoroplast that does not copolymerize with the mounting medium. It contains 50-60 functional cells which are numbered. The upper part of a cell has a cylindrical shape (55 mm in diameter, 2-3 mm in height) while its lower part is shaped like a truncated cone (this part is 2-3 mm deep). A drop of mount and a piece of tissue are placed in the cell, after which a solid fragment of the test specimen is introduced into its cylindrical part till the fragment's end abuts against the wall of the conical part of the cell and comes in contact with the mount. The two are then caused to polymerize, and once the polymerization is completed, the ready specimens can be readily extracted by taking hold of the distal ends of the solid fragments that protrude over the surface of the matrix.
Sidorkin
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D006801:Humans; D008867:Microtomy / Q000295:instrumentation* / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods
Matritsa i sposob izgotovleniia dvukhfragmentnykh obraztsov dlia ul'tramikrotomii.