An automated perifusion system for the study of rat spermatogenesis in vitro.
We report details of an automated perifusion system for the study of spermatogenesis in vitro. A potential of this new system is the evaluation of hormone/growth factor effects on the mitotic clonal expansion of spermatogonia and the meiotic and postmeiotic clonal differentiation of spermatogenic cells in coculture with Sertoli cells and in incubated seminiferous tubular segments.
Kierszenbaum
A L
AL
Department of Anatomy, School of Medicine, University of North Carolina, Chapel Hill 27514.
Tres
L L
LL
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D066298:In Vitro Techniques; D008297:Male; D008540:Meiosis; D008938:Mitosis; D010477:Perfusion / Q000295:instrumentation*; D051381:Rats; D012708:Sertoli Cells / Q000502:physiology*; D013091:Spermatogenesis; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000166:cytology*
D003470:Culture Media