title: [Construction of DNA-probes and their immunochemical detection using nonradioactive hybridization tests].
abstract: Simple and efficient chemical approaches to preparation of DNA probes carrying 2,4-dinitrophenyl, dansyl or biotin residues were developed. The residues were introduced using following DNA derivatization procedures: a) transamination of cytidine residues with O-(4-aminobutyl)hydroxylamine; b) mercuration of pyrimidine residues followed by beta-mercaptoethanol modification. It was shown that 2,4-dinitrophenyl-containing DNA probes can be used for nonradioactive hybridization detection of nucleic acids. DNP-DNA: DNA complexes were detected using mouse antibodies specific to 2,4-dinitrophenyl groups, which were developed with peroxidase-conjugated antimouse immunoglobulins. Peroxidase-catalyzed chemoluminescent reaction of luminol oxidation with hydrogen peroxide allowed to detect 10 picograms of the dinitrophenylated single-stranded DNA probe.
authors.forename: E B
authors.initials: EB
authors.lastname: Brosalina
authors.forename: V V
authors.initials: VV
authors.lastname: Vlasov
authors.forename: S A
authors.initials: SA
authors.lastname: Grachev
authors.forename: E N
authors.initials: EN
authors.lastname: Demchenko
mesh_terms: D001710:Biotin; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000276:immunology; D003619:Dansyl Compounds; D004136:Dinitrobenzenes; D006241:Haptens; D009693:Nucleic Acid Hybridization; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis
chemical_list: D003619:Dansyl Compounds; D004136:Dinitrobenzenes; D006241:Haptens; D011089:Polydeoxyribonucleotides; D001710:Biotin; D004247:DNA
vernacular_title: Konstruirovanie DNK-zondov i ikh immunokhimicheskoe opredelenie v neradioaktivnykh gibridizatsionnykh testakh.