Growth requirements of murine bone marrow macrophages in serum-free cell culture.
A serum-free medium for mass production of pure murine bone marrow-derived macrophages in liquid culture was developed. Iscove's modified Dulbecco's medium (IMDM) was used as a basal nutrient medium supplemented with 2-mercaptoethanol, transferrin and a source of colony-stimulating factor (CSF). Addition of exogenous lipids or bovine serum albumin was not required. The macrophages can be kept viable for at least eight days in the serum-free culture medium, and they are able to incorporate tritiated thymidine. The tritiated thymidine incorporation can be enhanced by retinoic acid, phorbol myristate acetate (PMA), colony-stimulating factor (CSF) and transferrin. Prostaglandin E1, prostaglandin E2 and dexamethasone inhibited tritiated thymidine incorporation. The serum-free culture of bone marrow-derived macrophages has some important implications. First, signal molecules can be defined which enhance or inhibit the proliferative capacity of bone marrow-derived macrophages. Second, the fatty acid composition of membrane phospholipids can be altered to study the relationship between macrophage function and membrane lipid composition.
Flesch
I
I
Ferber
E
E
D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D004261:DNA Replication; D003907:Dexamethasone / Q000494:pharmacology; D015232:Dinoprostone; D005260:Female; D007700:Kinetics; D007739:L Cells / Q000166:cytology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D011458:Prostaglandins E / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
D003470:Culture Media; D011458:Prostaglandins E; D014212:Tretinoin; D003907:Dexamethasone; D000527:Alprostadil; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine