title: Activities derived from established human myeloid cell lines reverse the suppression of cell line colony formation by lactoferrin and transferrin.
abstract: Myeloid cell lines were evaluated for the release of substances needed for colony formation by their own colony-forming cells (CFC) and by other myeloid cell lines. Dialyzed U937 conditioned medium (CM) had no effect on the cloning efficiency of U937 cells, whether or not U937 CFC had been induced for MHC class-II antigens by preincubation of these cells for 72 h with indomethacin and human gamma interferon (HuIFN gamma). Dialyzed U937 CM, however, restored colony formation of HuIFN gamma-induced U937 cells suppressed by lactoferrin (LF) or transferrin (TF). Dialyzed U937 CM did not restore colony formation of U937 cells suppressed by acidic isoferritins (AIF) or prostaglandin E2 (PGE2). Detection of the growth-restoring effects of U937 CM required that U937 CM be prepared in the presence of indomethacin or that the CM be dialyzed to remove inhibitors of U937 colony formation. Dialyzed U937 CM did not inactivate LF. Dialyzed U937 CM did not stimulate or enhance colony formation of normal human bone marrow granulocyte-macrophage (CFU-GM), erythroid (BFU-E), or multipotential (CFU-GEMM) progenitor cells, but did contain potent inhibitory activity against these progenitor cells. HL-60, EM2, EM3, and K562 cells were also evaluated. HL-60-, EM3-, and K562-CFC that were not preincubated with HuIFN gamma did not express MHC class-II antigens, and colony formation by these cells was not influenced by LF, TF, or AIF. Noninduced EM2-CFC constitutively expressed MHC class-II antigens, and colony formation by these cells was suppressed by LF, TF, and AIF. After induction of MHC class-II antigens on HL-60- and EM3-CFC by HuIFN gamma, colony formation by these cells was suppressed by LF, TF, and AIF. Colony formation by HuIFN gamma-induced EM2 cells was more responsive to inhibition by LF, TF, and AIF than was colony formation by noninduced EM2 cells. K562 cells were not induced into a responsive state to LF, TF, or AIF by HuIFN gamma. Dialyzed CM from HL-60, EM2, and EM3 cells contained activities that restored colony formation by their own LF-suppressed CFC. The activities present in dialyzed CM from U937, HL-60, EM2, and EM3 cells may be similar since they could each restore LF-suppressed colony formation of U937, HL-60, EM2, or EM3 cells.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.forename: H E
authors.initials: HE
authors.lastname: Broxmeyer
authors.forename: B Y
authors.initials: BY
authors.lastname: Rubin
authors.forename: E
authors.initials: E
authors.lastname: Berman
authors.forename: L
authors.initials: L
authors.lastname: Juliano
authors.forename: L
authors.initials: L
authors.lastname: Lu
authors.forename: L J
authors.initials: LJ
authors.lastname: Hast
authors.forename: S
authors.initials: S
authors.lastname: Cooper
authors.forename: J W
authors.initials: JW
authors.lastname: Singer
mesh_terms: D002460:Cell Line; D003114:Colony-Forming Units Assay; D003165:Complement System Proteins / Q000276:immunology; D003470:Culture Media; D005293:Ferritins / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007781:Lactoferrin / Q000494:pharmacology*; D007782:Lactoglobulins / Q000494:pharmacology*; D009000:Monocytes / Q000502:physiology; D011458:Prostaglandins E / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D000949:Histocompatibility Antigens Class II; D007782:Lactoglobulins; D011458:Prostaglandins E; D014168:Transferrin; D003165:Complement System Proteins; D005293:Ferritins; D007781:Lactoferrin