Structural studies of cell wall polysaccharides from Bifidobacterium breve YIT 4010 and related Bifidobacterium species.
The chemical compositions of the cell walls obtained from 8 strains in 5 species of Bifidobacterium were analyzed. These cell walls were shown to be composed of peptidoglycan and polysaccharide moieties. Some variations with respect to contents of neutral sugars and content of phosphorus were observed with some cell wall preparations from the same species. The neutral polysaccharides in cell walls of 4 strains of Bifidobacterium (B. bifidum YIT 4007, B. breve YIT 4010, B. infantis YIT 4025, and B. longum ATCC 15707) were purified and their chemical structures were analyzed. One of these polysaccharides, obtained from B. breve YIT 4010, was analyzed in detail by GLC, 1H- and 13C-NMR spectroscopic analyses, methylation, Smith degradation and acetolysis, and the results suggested the following structure for the repeating unit of the polysaccharide: (Formula: see text).
Habu
Y
Y
Institute of Immunological Science, Hokkaido University.
Nagaoka
M
M
Yokokura
T
T
Azuma
I
I
D000596:Amino Acids / Q000032:analysis; D001644:Bifidobacterium / Q000032:analysis*; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D002845:Chromatography; D002849:Chromatography, Gas; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009113:Muramidase / Q000378:metabolism; D010457:Peptidoglycan / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*
D000596:Amino Acids; D002241:Carbohydrates; D010457:Peptidoglycan; D011135:Polysaccharides, Bacterial; D009113:Muramidase