Expression of neuronal acetylcholine receptor polypeptides in vitro.
1. Translation of poly(A) RNA extracted from the nervous tissue of locusts in a reticulocyte lysate system led to polypeptides with a broad spectrum of molecular weights. 2. Using anti-locust acetylcholine receptor (AChR) antisera, polypeptides with a molecular weight of about 50,000 were immunoprecipitated. These peptides comprised about 0.3% of the total translation products. 3. Cotranslational incubation with pancreatic rough microsomes resulted in a glycosylated 60,000-dalton immunoprecipitate. 4. Density-gradient analysis of in vitro synthesized and glycosylated receptor polypeptides indicated that no assembly of subunits had taken the place under the in vitro conditions.
Benke
D
D
Department of Zoophysiology, University of Osnabrück, West Germany.
Breer
H
H
D000818:Animals; D006110:Grasshoppers / Q000378:metabolism*; D008970:Molecular Weight; D009474:Neurons / Q000378:metabolism*; D010455:Peptides / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000032:analysis / Q000235:genetics*
D010455:Peptides; D012333:RNA, Messenger; D011950:Receptors, Cholinergic