Monocytoid leukemia cell line CTV-1: morphological, immunological and isoenzymatic characteristics.
The human leukemia cell line CTV-1 was established from a case of acute monoblastic leukemia (AMoL). We analyzed the phenotypic marker profile of the CTV-1 cells in their original, untreated state and during induction of differentiation with the phorbolester 12-0-tetradecanoylphorbol 13-acetate (TPA). TPA led to morphological changes with signs of differentiation. Cell proliferation decreased in a dose-dependent fashion during exposure to TPA. In the surface marker analysis using a panel of 45 monoclonal antibodies (MoAbs) and several polyclonal antisera, CTV-1 cells were negative for markers of the T- and B-cell lineages, and were positive for several, but not all, myelomonocytic markers. Although the cells were reactive with the MoAb Leu-7 which identifies natural killer (NK) T-cells, no NK activity was detected. In the isoenzyme analysis of the four enzymes carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase (LDH) performed by isoelectric focusing on polyacrylamide gels, CTV-1 cells displayed isoenzyme profiles of immature myeloid cells. The overall marker profile of CTV-1 cells demonstrated cells of monocytoid origin arrested at a very early stage of differentiation, possibly close to the stage of precursor cells. As compared to other myelomonocytic cell lines, CTV-1 cells showed unusual morphological, immunological, functional and biochemical features and appeared to be relatively insensitive to treatment with TPA, although some alterations of the phenotype could be induced.
Drexler
H G
HG
Gaedicke
G
G
Maeda
S
S
Chen
P M
PM
Minowada
J
J
D000135:Acid Phosphatase / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006596:Hexosaminidases / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007694:Killer Cells, Natural / Q000276:immunology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D007948:Leukemia, Monocytic, Acute / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000954:Antigens, Surface; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D002265:Carboxylic Ester Hydrolases; D000135:Acid Phosphatase; D006596:Hexosaminidases; D013755:Tetradecanoylphorbol Acetate