title: In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation.
abstract: Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.
authors.forename: R
authors.initials: R
authors.lastname: Narayanan
authors.forename: M M
authors.initials: MM
authors.lastname: Jastreboff
authors.forename: C F
authors.initials: CF
authors.lastname: Chiu
authors.forename: J R
authors.initials: JR
authors.lastname: Bertino
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D015500:Chloramphenicol O-Acetyltransferase; D003114:Colony-Forming Units Assay; D004560:Electricity; D005260:Female; D006098:Granulocytes / Q000201:enzymology; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D008808:Mice, Inbred CBA; D010957:Plasmids; D014162:Transfection
chemical_list: D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase