title: Human adenovirus cloning vectors based on infectious bacterial plasmids.
abstract: By making use of the fact that human adenovirus DNA circularizes in infected cells, and that circular forms of the viral genome are infectious, we have developed an improved adenovirus-based cloning system. A deletion mutant of adenovirus type 5 (Ad5) with deletions in early regions 1 (E1) and 3 (E3) was converted to a bacterial plasmid which can regenerate infectious virus following transfection into human 293 cells. A single XbaI recognition site in the deleted E3 region serves as a site for the insertion of foreign DNA. We have used this system to clone a number of genes into the Ad5 genome and describe the insertion of the neomycin/G418 resistance marker into Ad5 as an example.
authors.forename: G
authors.initials: G
authors.lastname: Ghosh-Choudhury
authors.forename: Y
authors.initials: Y
authors.lastname: Haj-Ahmad
authors.forename: P
authors.initials: P
authors.lastname: Brinkley
authors.forename: J
authors.initials: J
authors.lastname: Rudy
authors.forename: F L
authors.initials: FL
authors.lastname: Graham
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004352:Drug Resistance, Microbial; D005814:Genes, Viral; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D010957:Plasmids; D014779:Virus Replication