Adrenaline stimulation of phospholipid metabolism in adipocyte ghosts.
The incorporation of long-chain fatty acids into phospholipids has been detected in adipocyte ghosts that were incubated with [1-14 C] stearic, [1-14 C] linoleic or [1-14 C] arachidonic acid. Adrenaline and adenosine activated this incorporation within 15 s of exposure of the ghosts to the hormones and the response was dose dependent. Maximum incorporation of labelled linoleic acid occurred at 10(-5) M adrenaline and 10(-7) M adenosine. The alpha-agonist phenylephrine and the beta-agonist isoproterenol were also shown to stimulate the incorporation of fatty acid in a dose dependent manner. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol were each labelled preferentially with linoleic or arachidonic acid. p-Bromophenacylbromide, quinacrine and centrophenoxine inhibited the adrenaline-stimulated incorporation of fatty acids into ghost membrane phospholipids, and p-bromophenacylbromide also reduced the activation of adenylate cyclase by adrenaline. NaF, an activator of adenylate cyclase, like adrenaline, stimulated the incorporation of linoleic acid into ghost membrane phospholipids.
Mann
D
D
Department of Biochemistry, University of Sydney, N.S.W.
Bersten
A M
AM
D000098:Acetophenones / Q000494:pharmacology; D000215:Acylation; D000241:Adenosine / Q000494:pharmacology; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010741:Phospholipases A / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000098:Acetophenones; D005227:Fatty Acids; D010743:Phospholipids; D010741:Phospholipases A; D000241:Adenosine; C006821:4-bromophenacyl bromide; D004837:Epinephrine