The tumor promoter phorbol 12-myristate 13-acetate induces a program of altered gene expression similar to that induced by platelet-derived growth factor and transforming oncogenes.
Treatment of mouse NIH 3T3 cells with the phorbol ester tumor promoter, phorbol 12-myristate 13-acetate, results in altered transcription of several genes as measured in nuclear run-off experiments. The first set of genes, whose altered transcription occurs rapidly in the absence of protein synthesis, is typified by induction of c-myc and c-fos and decreased transcription of alpha 2 type I procollagen. This work demonstrates the existence of a second class of genes whose rapidly increased transcription requires prior protein synthesis, which is represented by the gene encoding a secreted lysosomal protein, MEP. Similar induction of MEP RNA is seen after treatment with platelet-derived growth factor or transformation with Kirsten sarcoma virus.
Rabin
M S
MS
Doherty
P J
PJ
Gottesman
M M
MM
D000199:Actins / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003094:Collagen / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008562:Membrane Glycoproteins; D051379:Mice; D009857:Oncogenes; D010704:Phorbols / Q000494:pharmacology*; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014404:Tubulin / Q000235:genetics
D000199:Actins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010704:Phorbols; D010750:Phosphoproteins; D010982:Platelet-Derived Growth Factor; D011506:Proteins; D012333:RNA, Messenger; D014404:Tubulin; C018589:lysosomal proteins; C025363:mannoproteins; D003094:Collagen; D013755:Tetradecanoylphorbol Acetate