title: Structure activity studies on the induction of peroxisomal enzyme activities by a series of phthalate monoesters in primary rat hepatocyte cultures.
abstract: A series of 9 phthalate monoesters were cultured with primary rat hepatocytes for 70 h and their effect on peroxisomal fatty acid beta-oxidation (palmitoyl-CoA oxidation) determined. Marked quantitative differences in the induction of enzyme activity were observed with both alkyl chain length and position of side chain substitution affecting compound potency. With all 9 compounds a good correlation was observed between electronic structural parameters obtained by molecular orbital calculations and biological activity in the cell culture system. These results demonstrate a relationship between chemical structure and biological activity for a series of phthalate monoesters and indicate the potential usefulness of primary hepatocyte cultures to screen compounds for peroxisome proliferation.
authors.forename: B G
authors.initials: BG
authors.lastname: Lake
authors.forename: D F
authors.initials: DF
authors.lastname: Lewis
authors.forename: T J
authors.initials: TJ
authors.lastname: Gray
authors.forename: J A
authors.initials: JA
authors.lastname: Beamand
authors.forename: K D
authors.initials: KD
authors.lastname: Hodder
authors.forename: R
authors.initials: R
authors.lastname: Purchase
authors.forename: S D
authors.initials: SD
authors.lastname: Gangolli
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D008830:Microbodies / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000378:metabolism*; D010795:Phthalic Acids / Q000633:toxicity*; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D000214:Acyl Coenzyme A; D010795:Phthalic Acids; D010171:Palmitoyl Coenzyme A