title: Purification, ultrastructure, and chemical analysis of Alzheimer disease amyloid plaque core protein.
abstract: Isolation of Alzheimer disease amyloid plaque core protein (APCP) was carried out by repetitive NaDodSO4/EDTA/sucrose extractions and by Ficoll-400 density-gradient centrifugations. The enriched APCP-Ficoll interface was labeled with the fluorochrome thioflavin T and separated from the contaminating lipofuscin by fluorescence-activated cell sorting. Electron microscopy demonstrated that APCP is made of two different kinds of filaments measuring 5.5-6 nm and 10-12 nm, respectively, and of variable length. Purified APCP and lipofuscin were chemically modified by performic acid oxidation. The amino acid composition of APCP revealed a high content of glycine and valine (30%) and 1% cysteine. By contrast, the protein moiety of the copurified lipofuscin contained 16% cysteine. The amino acid composition of APCP did not resemble that of any known protein.
authors.forename: A
authors.initials: A
authors.lastname: Roher
authors.forename: D
authors.initials: D
authors.lastname: Wolfe
authors.forename: M
authors.initials: M
authors.lastname: Palutke
authors.forename: D
authors.initials: D
authors.lastname: KuKuruga
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000682:Amyloid / Q000302:isolation & purification* / Q000378:metabolism; D001923:Brain Chemistry; D005434:Flow Cytometry; D006801:Humans; D008062:Lipofuscin / Q000302:isolation & purification; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility
chemical_list: D000596:Amino Acids; D000682:Amyloid; D008062:Lipofuscin; D009419:Nerve Tissue Proteins