title: High frequency of N-ras activation in acute myelogenous leukemia.
abstract: Using the NIH/3T3 cell transfection assay, activated cellular oncogenes have been detected in around 10% to 20% of human tumors. From a series of DNA preparations from tissues infiltrated with acute myelogenous leukemia (AML), 50% (3/6) caused transformation of NIH/3T3 cells. Thus AML appears to be the human tumor with the highest frequency of oncogenes detected by DNA transfection. In each case the oncogene involved was N-ras, a member of the ras gene family. Biologic and clinical parameters of AML patients with and without N-ras oncogenes in their tumors are discussed.
authors.forename: S W
authors.initials: SW
authors.lastname: Needleman
authors.forename: M H
authors.initials: MH
authors.lastname: Kraus
authors.forename: S K
authors.initials: SK
authors.lastname: Srivastava
authors.forename: P H
authors.initials: PH
authors.lastname: Levine
authors.forename: S A
authors.initials: SA
authors.lastname: Aaronson
mesh_terms: D000293:Adolescent; D000818:Animals; D002869:Chromosome Aberrations; D005260:Female; D005784:Gene Amplification; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D051379:Mice; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011519:Proto-Oncogenes; D011995:Recombination, Genetic; D014170:Transformation, Genetic