Specific serodiagnosis with adult Onchocerca volvulus antigen in an enzyme-linked immunosorbent assay.
The cross-reactivity of the blood from onchocerciasis, loiasis, and dipetalonemiasis was tested by a micro-ELISA technique, utilizing adult Onchocerca volvulus antigen and blood samples taken on filter paper. The average ELISA values (OD at 500 nm) were as follows: 0.58 in persons with O. volvulus microfilariae (n = 81), 0.49 in microfilariae-negatives from the same endemic area (n = 39), 0.15 in dipetalonemiasis (n = 27), and 0.25 in loiasis (n = 12), while those of 65 Dipetalonema perstans-negative people were markedly low (average 0.14) and that of 22 Loa loa-negatives, 0.22, respectively. This ELISA could successfully differentiate onchocerciasis from dipetalonemiasis and loiasis.
Tada
I
I
Korenaga
M
M
Shiwaku
K
K
Ogunba
E O
EO
Ufomadu
G O
GO
Nwoke
B E
BE
D000293:Adolescent; D000328:Adult; D000368:Aged; D000947:Antigens, Helminth / Q000032:analysis*; D004154:Dipetalonema Infections / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008118:Loiasis / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009854:Onchocerca / Q000276:immunology*; D009855:Onchocerciasis / Q000175:diagnosis* / Q000276:immunology; D012698:Serologic Tests
D000947:Antigens, Helminth