Photochemiluminescent detection of antiradical activity. I. Assay of superoxide dismutase.
The luminol sensitized photogeneration of superoxide radicals was combined with their luminol-dependent chemiluminescent detection. The inhibition of chemiluminescence by superoxide dismutase (SOD) versus enzyme concentration in reciprocals was strongly linear. The amount of SOD notable for 50% inhibition was between 115 and 500 ng for different enzyme preparations.
Popov
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IN
Institute of Medical Immunology, Humboldt University, Berlin, GDR.
Lewin
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G
von Baehr
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R
D004912:Erythrocytes / Q000201:enzymology; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*
D005609:Free Radicals; D008165:Luminol; D013482:Superoxide Dismutase