Determination of doxorubicin and doxorubicin-3-ol in rat heart.
The discovery of a delayed form of DXR-induced cardiotoxicity raises the question whether the presence of the drug or of metabolites in myocardial cells is necessary for the development of cardiotoxicity. The present investigations deal with a new method for the determination of DXR and of its main metabolite, DXR-3-ol, in cardiac cells. Rat hearts "ex-vivo", isolated 24 h after i.v. administration of 6 mg/kg DXR and 20 micro C of DXR-14C, were perfused with ice-cold Tyrode solution, which releases anthracyclines from extracellular spaces. After homogenization DXR and DXR-3-ol were extracted from the cell at room temperature. The separation was carried out by HPLC; the recovery was about 95%, measured by the extracted radioactivity compared with that of the pellet residue.
Rossini
L
L
Monti
E
E
Cova
D
D
Piccinini
F
F
D000818:Animals; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000633:toxicity; D006321:Heart / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004317:Doxorubicin; C010013:adriamycinol