Deletion analysis of the c-Ha-ras oncogene promoter.
Using transient assays of chloramphenicol acetyltransferase expression in CV-1 cells, we have localized the human c-Ha-ras oncogene promoter to a 550 bp fragment located about 1 kb upstream from the ras coding sequence. Deletion analysis has revealed that a 100 nucleotide region 200 base pairs upstream of the putative initiation sites for transcription is essential for high levels of expression. Within this sequence lie two Spl binding sites and a consensus CCAAT box.
Trimble
W S
WS
Hozumi
N
N
D000123:Acetyltransferases / Q000032:analysis / Q000235:genetics*; D001482:Base Composition; D015500:Chloramphenicol O-Acetyltransferase; D002855:Chromatography, Thin Layer; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006801:Humans; D009857:Oncogenes; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase