title: Purification and characterization of the alpha form of rat plasma kallikrein.
abstract: A four-step procedure was used to purify rat plasma kallikrein (RPK) with a relative molecular mass (Mr) of 87 kD (obtained both by gel filtration and SDS-PAGE), which indicates the purification of an alpha (intact) kallikrein, in contrast to previously described RPK preparations which had lower Mr (beta or degraded form). RPK is a neutral (pI 6.7) serine proteinase glycoprotein (15% carbohydrates) and contains (residues/mol): galactose (27), N-acetylglucosamine (24), mannose (13), glucose (13) and fucose (7). This purified alpha form of RPK has properties very similar to those of pure human and bovine kallikreins.
authors.affiliation: Departamento de Bioquímica, Escola Paulista de Medicina, São Paulo, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Kouyoumdjian
authors.forename: D R
authors.initials: DR
authors.lastname: Borges
authors.forename: Y M
authors.initials: YM
authors.lastname: Michelacci
authors.forename: J A
authors.initials: JA
authors.lastname: Guimarães
authors.forename: C A
authors.initials: CA
authors.lastname: Sampaio
authors.forename: J L
authors.initials: JL
authors.lastname: Prado
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007610:Kallikreins / Q000097:blood / Q000302:isolation & purification*; D008968:Molecular Conformation; D051381:Rats
chemical_list: D007610:Kallikreins