title: Dynamic analysis of lymphocyte migration into Peyer's patches of rat small intestine.
abstract: Dynamic aspects of lymphocyte immigration into Peyer's patches were analyzed in rat small intestine using intravital fluorescence microscopy. Lymphocytes from intestine, lymph, and spleen were labeled with acridine orange in vitro and infused from the mesenteric artery of rats. There are three types of lymphocytes as characterized from their interaction with post-capillary venules of Peyer's patches immediately after infusion, and most of the lymphocytes passed through the microcirculatory bed without any interaction with venules. About 15 to 20 minutes later, the number of lymphocytes which stick to the Peyer's patches was increased and reached a maximum at 40 to 50 minutes. There was a difference in distribution pattern of infused lymphocytes between spleen cells and lymphocytes from intestinal lymph suggesting the complex control mechanism of lymphocyte migration into Peyer's patches.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Miura
authors.forename: H
authors.initials: H
authors.lastname: Asakura
authors.forename: M
authors.initials: M
authors.lastname: Tsuchiya
mesh_terms: D000818:Animals; D002465:Cell Movement; D007421:Intestine, Small / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology*; D008856:Microscopy, Fluorescence; D010581:Peyer's Patches / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains