Polymorphonuclear leukocyte histamine receptors: occurrence in cell surface clusters and their redistribution during locomotion.
A univalent and bioactive fluorescent derivative of histamine bound to the surface of human polymorphonuclear leukocytes; free histamine was found to compete with this derivative for binding sites. Histamine H2-receptor specificity was indicated by binding inhibition experiments using cimetidine (H2-specific) but not diphenhydramine (H1-specific). Video-intensification fluorescence microscopy was used to determine the distribution of histamine receptors in living polymorphonuclear leukocytes. Receptors appeared as randomly distributed clusters upon stationary cells. During random locomotion, receptors were restricted to the ends of pseudopods, whereas chemotaxis led to receptor localization at lamellipodia and uropods. Ligand-receptor complexes were restricted to the cell surface, as shown by quenching exterior fluorescence with crystal violet. Therefore, pinocytic uptake cannot account for the observed receptor localization or clustering. As a further control, the lipid analog 1,1-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine remained uniformly distributed during all conditions. Histamine-mediated inhibition of adherence may be related to formation of ligand-receptor membrane domains at adherence sites.
Petty
H R
HR
Francis
J W
JW
D002451:Cell Compartmentation; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002465:Cell Movement; D005456:Fluorescent Dyes; D006632:Histamine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000378:metabolism*; D011968:Receptors, Histamine / Q000378:metabolism*
D005456:Fluorescent Dyes; D011968:Receptors, Histamine; D006632:Histamine