Characterization of rabbit cytochrome P450IIC4 cDNA and induction by phenobarbital of related hepatic mRNA levels.
We have cloned cDNA containing a partial sequence of a rabbit cytochrome P-450 (designated cytochrome P450IIC4) cDNA that is a member of the cytochrome P450IIC subfamily. The cDNA contains 770 bp of which the first 429 code for the C-terminal 143 amino acids of cytochrome P450IIC4. The protein coding region of the cDNA is 98% homologous with that of cytochrome P450IIC5 and the 3' untranslated region is about 90% homologous. In contrast to the constitutive isozyme, cytochrome P450IIC5, mRNA in the liver that hybridized to the 3' untranslated region of cytochrome P450IIC4 cDNA was increased about 8-fold 24 hours after a single injection of phenobarbital.
Zhao
J
J
Leighton
J K
JK
Kemper
B
B
D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D004790:Enzyme Induction; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009693:Nucleic Acid Hybridization; D010634:Phenobarbital / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits
D012333:RNA, Messenger; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital