title: Secondary activation of c-abl may be related to translocation to the nucleolar organizer region in an in vitro cultured rat leukemia cell line (K3D).
abstract: Localization of cellular oncogenes (c-onc) near the break points of translocations in tumor cells has indicated involvement of these genes in neoplastic growth. Enhanced transcription of the cellular homolog (c-abl) of the transforming sequence of Abelson murine leukemia virus was observed in K3D, which was one of the cloned cell lines of 7,12-dimethylbenz[a]anthracene-induced rat erythroblastic leukemia. Since the c-abl activation was not observed in the parent cell line (K2D) from which K3D was derived and the latter was different from the former in the presence of a new marker chromosome, t(3;12), this marker may play a role in the expression of c-abl in K3D cells. In contrast to the human c-onc assignments, few rat c-onc assignments have been reported. In situ molecular hybridization studies assigned c-abl to the 3q12 site of the normal chromosome 3 and to the break point of the translocation t(3;12) in K3D cells. Another break point in this translocation chromosome 12p11 involves the nucleolar region, and the 3;12 translocation may involve c-abl and nucleolar cistrons. These results provide evidence of secondary c-onc activation during karyotypic evolution of cloned malignant cells.
authors.forename: R
authors.initials: R
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Mihara
authors.forename: S
authors.initials: S
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: H L
authors.initials: HL
authors.lastname: Chen
authors.forename: N
authors.initials: N
authors.lastname: Aoyama
authors.forename: S
authors.initials: S
authors.lastname: Murao
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D000011:Abelson murine leukemia virus / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005819:Genetic Markers; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009697:Nucleolus Organizer Region; D009857:Oncogenes; D011519:Proto-Oncogenes; D051381:Rats; D014178:Translocation, Genetic
chemical_list: D004273:DNA, Neoplasm; D004274:DNA, Recombinant; D005819:Genetic Markers