A simplified method for the iodination of prostanoids for radioimmunoassay.
This paper presents a simplified method for iodination of prostanoids which combines conjugation and iodination into one brief step. The prostanoid is first coupled to histamine with EDC, and is thereafter iodinated directly without an intervening TLC step. The iodination mixture is purified on an LH-20 column, and the final iodinated product can be located unequivocally. The simplified procedure presented here should make iodinated tracers accessible to more laboratories.
Uderman
H D
HD
Workman
R J
RJ
D002700:Chloramines; D006632:Histamine; D007457:Iodine Radioisotopes; D011453:Prostaglandins / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D013931:Thromboxanes; D014105:Tosyl Compounds
D002700:Chloramines; D007457:Iodine Radioisotopes; D011453:Prostaglandins; D013931:Thromboxanes; D014105:Tosyl Compounds; C016300:chloramine-T; D006632:Histamine