title: Kinetically controlled enzyme-catalyzed synthesis of kyotorphin. An optimization study.
abstract: Regarding the alpha-chymotrypsin-catalyzed synthesis of Z-Tyr-Arg-NH2, the effect on the reaction yield of the following experimental factors is discussed: DMF/buffer proportions, reaction temperature, and donor/nucleophile ratios. The experimental design shows that relatively better yields are obtained by increasing the reaction temperature and lowering the cosolvent proportion while maintaining a slight excess of nucleophile. The ascensional line reaches an optimal response surface very flat voided of a well-defined maximum.
authors.affiliation: Biological Organic Chemistry Department, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Clapés
authors.forename: G
authors.initials: G
authors.lastname: Valencia
authors.forename: F
authors.initials: F
authors.lastname: Reig
authors.forename: J M
authors.initials: JM
authors.lastname: García Antón
authors.forename: J
authors.initials: J
authors.lastname: Mata
mesh_terms: D000700:Analgesics / Q000138:chemical synthesis*; D002918:Chymotrypsin / Q000378:metabolism*; D004723:Endorphins / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D007700:Kinetics; D013816:Thermodynamics
chemical_list: D000700:Analgesics; D004723:Endorphins; D007202:Indicators and Reagents; C019448:kyotorphin; D002918:Chymotrypsin