Detection of immunoglobulins and/or complement in kidney tissues from non-obese diabetic (NOD) mice.
Immunofluorescent studies were performed on kidney tissues from non-obese diabetic (NOD) mice. Tissues from twenty-two NOD mice ranging in age from 8 to 33 weeks were examined by immunofluorescence and light microscopy. It was shown that the immunofluorescent pattern of immunoglobulins and complement deposition in glomeruli from NOD mice were changed from diffuse linear, focal and/or local fine granular to diffuse coarse granular depending on the age. These findings in NOD mice were consistent with those in human diabetic nephropathy. It was suggested that the immunofluorescent studies in NOD mice are useful for evaluation of the pathogenesis of renal involvement in patients with diabetes mellitus.
Watanabe
S
S
Department of Internal Medicine, School of Medicine, Tokai University, Japan.
Tomino
Y
Y
Inoue
W
W
Yagame
M
M
Kaneshige
H
H
Nomoto
Y
Y
Sakai
H
H
D000818:Animals; D003176:Complement C3 / Q000032:analysis*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000209:etiology; D007136:Immunoglobulins / Q000032:analysis*; D007668:Kidney / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D003176:Complement C3; D007136:Immunoglobulins