Effects of coculture with uterine epithelial cells on the metabolism of glucose by mouse morulae and early blastocysts.
Coculture of mouse morulae/early blastocysts with isolated endometrial epithelial cells reduced incorporation of glucose carbon into embryonic glycogen but had no significant effect on incorporation into other internal carbon pools during a 5-h culture in serum-supplemented Dulbecco's modification of Eagle's minimum essential medium. Turnover of glycogen pools during 24-h chase culture of pulse-labelled embryos was unaffected by the presence of uterine epithelial cells recovered from day-4 pregnant or non-pregnant mice. However, significantly more label was retained in non-glycogen macromolecules during chase in the presence of endometrium recovered from non-pregnant than from pregnant uteri.
Khurana
N K
NK
School of Veterinary Studies, Murdoch University, W.A.
Wales
R G
RG
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002970:Cleavage Stage, Ovum / Q000378:metabolism*; D046508:Culture Techniques; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D051379:Mice; D009028:Morula / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
D006003:Glycogen; D005947:Glucose