title: Recombinant interferon alpha 2 stimulation of target-binding by natural killer cells.
abstract: Even though the enhancement of the lyitc capacity and the kinetics of lysis of natural killer cells (NK) by interferon has been well documented, an increase of the target-effector cell binding percentage is still disputed. We, therefore, modified the Grimm-Bonavida single-cell assay so that 400 to 600 cells per individual determination could be reliably evaluated. Using this assay, which makes possible separate determination of effector-target cell binding and target lysis, we demonstrated that, in addition to lytic capacity, target-effector cell binding is also increased by preincubating NK with 100 to 1,000 IU interferon alpha 2 per 10(6) cells. Our data indicate that interferon alpha 2 induces pre-NK cells to bind target cells and that it activates these pre-NK cells to kill the targets.
authors.forename: E
authors.initials: E
authors.lastname: Heidemann
authors.forename: J
authors.initials: J
authors.lastname: Weber
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: U
authors.initials: U
authors.lastname: Reichmann
mesh_terms: D000328:Adult; D001665:Binding Sites / Q000187:drug effects; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects*; D004915:Leukemia, Erythroblastic, Acute; D008297:Male; D011994:Recombinant Proteins / Q000494:pharmacology*
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins