Determination of 7-hydroxymethotrexate in human plasma by reversed phase high-performance liquid chromatography.
A rapid and sensitive reversed phase HPLC method was developed for the detection of 7-hydroxymethotrexate (7-OHMTX) which is the main metabolite in human plasma after high-dose methotrexate (MTX) therapy. The simple clean-up procedure of plasma samples described allows the measurement of 7-OHMTX during clinical management of MTX infusions. Elimination kinetics of 7-OHMTX are given from 53 drug cycles of 6 patients.
Erttmann
R
R
Bielack
S
S
Landbeck
G
G
D002648:Child; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007700:Kinetics; D008727:Methotrexate / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000627:therapeutic use; D012516:Osteosarcoma / Q000188:drug therapy; D013997:Time Factors
C011864:7-hydroxymethotrexate; D008727:Methotrexate