Production of new hybrid antibiotics, mederrhodins A and B, by a genetically engineered strain.
Hybrid antibiotics mederrhodins A and B were produced by a recombinant strain consisting of the medermycin-producing Streptomyces sp. strain AM7161 containing part of the gene clusters for actinorhodin biosynthesis of Streptomyces coelicolor A3(2). Mederrhodin A has a hydroxyl group at the C-6 position of the medermycin molecule, and mederrhodin B is dihydromederrhodin A. The antimicrobial activity of mederrhodin A resembled that of medermycin. Mederrhodin B was almost devoid of antimicrobial activity.
Omura
S
S
Ikeda
H
H
Malpartida
F
F
Kieser
H M
HM
Hopwood
D A
DA
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005285:Fermentation; D005818:Genetic Engineering; D008826:Microbial Sensitivity Tests; D009285:Naphthoquinones / Q000096:biosynthesis / Q000494:pharmacology; D011808:Quinone Reductases / Q000032:analysis / Q000302:isolation & purification; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D009285:Naphthoquinones; C049398:mederrhodin B; C049397:mederrhodin A; D011808:Quinone Reductases; C049396:isochromanequinone reductase