Spermatozoal nuclear chromatin decondensation in vitro: a test for sperm immaturity. Comparison with results of human in vitro fertilisation.
Human sperm immaturity was tested by nuclear chromatin decondensation (NCD) in 1% sodium dodecylsulphate (SDS) of spermatozoa used in 74 consecutive IVF treatments. NCD was significantly higher in the presence of 2 mmol/l dithiothreitol (DTT) and after washing sperm from semen with 6 mmol/l ethylenediaminetetraacetic acid (EDTA). NCD was significantly less in insemination suspensions prepared by the swim-up technique than in the original semen. NCD with DTT was inversely correlated with sperm motility and motility index but there were no significant relationships between NCD and other semen analysis variables. There was no significant correlation between any NCD test and the proportion of oocytes fertilised in vitro. The only factors being significantly correlated with the fertilisation rate were proportion of sperm with normal morphology (Kendall correlation, tau = 0.36, P less than 0.001) and sperm concentration (tau = 0.14, P less than 0.05). Logistic regression analysis of fertilisation rates showed that only percentage normal sperm morphology was significant. It is concluded that NCD does not provide additional useful clinical information about sperm fertilising ability in vitro.
Liu
D Y
DY
Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia.
Elton
R A
RA
Johnston
W I
WI
Baker
H W
HW
D002467:Cell Nucleus / Q000502:physiology*; D004229:Dithiothreitol / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D012728:Sex Chromatin / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013076:Sperm Count / Q000187:drug effects; D013079:Sperm Maturation / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology*
D012967:Sodium Dodecyl Sulfate; D004229:Dithiothreitol