[Isolation of the R'his plasmids of Vibrio cholerae].
V. cholerae strain VT5104 capable of donor activity in conjugation has been constructed by the genetic technique based on plasmid RP4::Mucts62 integration into V. cholerae chromosome due to plasmid homology with Mucts62 inserted into the chromosome. The gene for histidine synthesis has been mobilized and transferred into the recipient cells from VT5104 donor. The conjugants obtained are able to efficiently transfer his+ gene included into the plasmid structure in conjugation with eltor recipient. Thus, the constructed strain VT5104 generates R' plasmids carrying V. cholerae chromosomal genes.
Rusina
O Iu
OIu
Tiganova
I G
IG
Aleshkin
G I
GI
Andreeva
I V
IV
Skavronskaia
A G
AG
D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D005819:Genetic Markers; D006639:Histidine / Q000235:genetics*; D011815:R Factors; D011995:Recombination, Genetic; D013696:Temperature; D014734:Vibrio cholerae / Q000235:genetics*
D005819:Genetic Markers; D006639:Histidine
Poluchenie plazmid R'his Vibrio cholerae.