Determination of bovine enamel protein by enzyme-linked immuno-adsorbent assay.
A specific enzyme-linked immuno-adsorbent assay for enamel protein of the developing tooth was devised, using antibody against antigen prepared from immature bovine enamel. Samples containing 5-500 ng of amelogenin protein in 50 microliters gave consistent results. Bovine enamelin cross-reacted with the antibody but was less reactive than amelogenin. Proteins in other tissues or fluids did not react to the antibody.
Baba
T
T
Oida
S
S
Shimokawa
H
H
Sasaki
S
S
D053523:Amelogenin; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D003743:Dental Enamel / Q000254:growth & development; D003746:Dental Enamel Proteins / Q000032:analysis* / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay
D053523:Amelogenin; D003746:Dental Enamel Proteins; C038456:tuftelin