Effect of amphotericin B on human periodontal ligament cells cultured in vitro.
Three micrograms of amphotericin B/ml nutrient medium markedly inhibited the monolayer outgrowth of cells from explants of human periodontal ligament. Amphotericin B (1.5 microgram/ml) retarded outgrowth with morphological evidence of cell injury. The addition of 3 micrograms/ml amphotericin B to existing monolayers initially caused disruption of the majority of cells but those which survived then proliferated. Thus, routine use of amphotericin B is contra-indicated in tissue culture of human periodontal ligament.
Adams
A M
AM
Soames
J V
JV
Searle
R F
RF
D000666:Amphotericin B / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*
D000666:Amphotericin B