[Measurement of total bile acids. Comparison of different preparations of 3 alpha-hydroxysteroid dehydrogenase].
Oxidation of free and conjugated biliary acids was carried out with 5 different enzymatic preparations of 3 alpha-hydroxysteroid dehydrogenase. The comparison of the catalytic activities obtained, shows the existence of important variations according to the enzymes. Preparations purified by chromatography have a stronger activity toward primary biliary acids than toward secondary biliary acids, while preparations obtained from bacterial mutants have a low activity toward cholic acid and its conjugates.
Feldmann
D
D
Beaudonnet
A
A
Goudable
J
J
Petigny
B
B
Sarmini
H
H
Vercambre
C
C
D015096:3-Hydroxysteroid Dehydrogenases / Q000302:isolation & purification; D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis* / Q000097:blood; D002384:Catalysis; D006801:Humans; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000201:enzymology
D001647:Bile Acids and Salts; D015096:3-Hydroxysteroid Dehydrogenases
Dosage des acides biliaires totaux. Comparaison de différentes préparations de 3 alpha-hydroxystéroïde déshydrogénase.