Age dependent changes in the activity of the cytosolic fraction from rat liver to stimulate polysomal protein synthesis and the role of initiation factor eIF-2.
The protein synthesis stimulating activity of the cytosol from the livers of rats of different age was tested in a cell-free system using washed rat liver polysomes. This activity declines significantly with increasing age. From experiments on the effect of addition of purified eIF-2 to the cell-free polysomal/cytosolic systems as well as from changes in the Met-tRNA(f) binding activity of the cytosol it is concluded that alterations in eIF-2 play an essential role in the decrease of protein synthesis with increasing age.
Junghahn
I
I
Akademie der Wissenschaften der DDR, Zentralinstitut für Molekularbiologie, Berlin-Buch.
Bommer
U A
UA
D000375:Aging / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D003600:Cytosol / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D015852:Eukaryotic Initiation Factor-2; D005260:Female; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000502:physiology*; D011132:Polyribosomes / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors; D011506:Proteins