title: Identification of the 90 kDa substrate of rat liver type II casein kinase with the heat shock protein which binds steroid receptors.
abstract: It was recently reported that the type II casein kinase of rat liver cytosol co-purified with a major 90 kDa substrate when subjected to gel filtration at low ionic strength. The identity of the 90 kDa substrate was unknown. We have verified this report and have shown that the 90 kDa substrate is recognized by a monoclonal antibody prepared against the 90 kDa heat shock protein. This ubiquitous phosphoprotein is known to increase in abundance in cells subjected to heat stress and has been shown to complex steroid receptors and certain retrovirus tyrosine kinases.
authors.forename: J J
authors.initials: JJ
authors.lastname: Dougherty
authors.forename: D A
authors.initials: DA
authors.lastname: Rabideau
authors.forename: A M
authors.initials: AM
authors.lastname: Iannotti
authors.forename: W P
authors.initials: WP
authors.lastname: Sullivan
authors.forename: D O
authors.initials: DO
authors.lastname: Toft
mesh_terms: D000818:Animals; D047388:Casein Kinases; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011987:Receptors, Steroid / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D006360:Heat-Shock Proteins; D011987:Receptors, Steroid; D011494:Protein Kinases; D047388:Casein Kinases