[Cloning of DNA complementary to mRNA for proopiomelanocortin from the bovine, rat and human hypophysis. Hormonal regulation of proopiomelanocortin mRNA in the rat hypophysis].
Cloning of DNA and complementary mRNA of bovine, rat and human proopiomelanocortin (POMC) was carried out. A structural analysis of the cloned cDNA of POMC was performed. Using restriction fragments of bovine, rat and human POMC cloned cDNA, probes for molecular hybridization based on one-chain bacteriophage M13 were made. Using the dot-hybridization technique with labeled [32P] POMC cDNA, the effect of 17 beta-estradiol and adrenalectomy on the POMC mRNA level in rat hypophysis was studied. The hormone was shown to significantly decrease the POMC mRNA content at a concentration of 4 and 8 micrograms per 100 g of body weight 4 hours after injection. Adrenalectomy caused a 2-3-fold increase in the POMC mRNA level in rat hypophysis after a period of 5-7 days. The possibility of polyhormonal control of POMC genome transcription is discussed.
Mertvetsov
N P
NP
Golovin
S Ia
SIa
Beklemishev
A B
AB
Karginov
V A
VA
Mamaev
L V
LV
D000315:Adrenalectomy; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004958:Estradiol / Q000502:physiology; D005260:Female; D005819:Genetic Markers; D006801:Humans; D009693:Nucleic Acid Hybridization; D010902:Pituitary Gland / Q000032:analysis*; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014158:Transcription, Genetic
D005819:Genetic Markers; D012333:RNA, Messenger; D004958:Estradiol; D011333:Pro-Opiomelanocortin; D004247:DNA
Klonirovanie DNK, komplementarnoĭ mRNK proopiomelanokortina gipofiza byka, krysy i cheloveka. Gormonal'naia reguliatsiia soderzhaniia mRNK proopiomelanokortina v gipofize krysy.