Characterization and androgenic regulation of soluble protein kinases and protein phosphorylation in rat ventral prostate gland.
The soluble protein kinase activities for protamine and casein, the histone kinases modulated by cAMP or Ca2+ and phospholipid, as well as the phosphorylation patterns of endogenous proteins were measured in rat ventral prostates from normal adults, castrates, and dihydrotestosterone-treated castrates. In normal prostate, the ratio of cAMP-dependent type I and II kinases was approximately 1:5. After a 3-week period of castration-induced regression, the concentrations of both enzymes were increased, but on a total organ basis, type I was decreased to 56%, while type II was reduced to 20% of normal levels. Casein kinase activity in unfractionated cytosol was not significantly altered by castration but when partially resolved into type I and II enzymes, there appeared to be a selective reduction in the type I component. In contrast, the total organ activities of protamine kinase or Ca2+-activated, phospholipid-dependent kinase, two measures of protein kinase C enzyme, were significantly increased (64 and 71%, respectively) above sham controls in regressed organs of castrates. All of the castration-induced changes in protein kinases were restored toward normal by dihydrotestosterone treatment. Castration effects on protein kinase C and the cAMP-dependent kinases appeared to be manifest in the phosphorylation of endogenous proteins. Castration resulted in a qualitative shift in the cAMP-dependent phosphorylation patterns as measured by gel electrophoresis, with increases in four major bands and decreases in two others, whereas the Ca2+-activated, phospholipid-dependent phosphorylation patterns were all enhanced. It is concluded that the androgenic regulation of protein kinase C differed qualitatively from that of other kinases, and its activation upon withdrawal of the androgenic stimulus may be involved in autophagic mechanisms in the prostate.
Durham
J P
JP
Butcher
F R
FR
Teoh
T S
TS
Miller
R
R
Mawhinney
M G
MG
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D047388:Casein Kinases; D002369:Castration; D002848:Chromatography, DEAE-Cellulose; D013196:Dihydrotestosterone / Q000494:pharmacology; D008297:Male; D010766:Phosphorylation; D011467:Prostate / Q000378:metabolism*; D011493:Protein Kinase C / Q000032:analysis; D011494:Protein Kinases / Q000032:analysis*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility
D000728:Androgens; D011506:Proteins; D013196:Dihydrotestosterone; D011494:Protein Kinases; D047388:Casein Kinases; D011493:Protein Kinase C