title: Sodium-calcium exchange exists across the cell membrane of intact vascular smooth muscle cells.
abstract: Ouabain stimulated Ca2+ uptake in rat aortic smooth muscle cells in culture. The maximum uptake of Ca2+ was observed at about 200 mumol/l ouabain. Na+K+-ATPase activity of these cells was also maximally inhibited at about 200 mumol/l ouabain. The ouabain-stimulated Ca2+ uptake was not inhibited by the calcium antagonist diltiazem. The cells treated with ouabain also showed increased steady-state cytosolic Ca2+ concentration as measured by the fluorescent dye indicator Fura 2/AM. The ouabain stimulated Ca2+ uptake and increased cytosolic Ca2+ is explained as follows: ouabain inhibits Na+K+-ATPase with subsequent increase in cytosolic Na+, which is then exchanged with external Ca2+ through the Na+-Ca2+ exchange in intact vascular smooth muscle cells.
authors.forename: M A
authors.initials: MA
authors.lastname: Matlib
authors.forename: K R
authors.initials: KR
authors.lastname: Whitmer
authors.forename: E P
authors.initials: EP
authors.lastname: MacCarthy
authors.forename: B S
authors.initials: BS
authors.lastname: Ooi
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism*
chemical_list: D012964:Sodium; D002118:Calcium