Improved procedure for platelet freezing.
Platelets frozen in glycerol-glucose frequently aggregated on thawing or reconstitution. This has been eliminated by using a nonplasma diluent (pH 6.5) and a new freezing bag. The results were improved platelet 14C-serotonin uptake and readily demonstrable aggregation to ADP, epinephrine, collagen and thrombin. 14C-serotonin uptake was at maximum when platelets were frozen at thrombocrits of up to 15%. Hemostatic effectiveness was demonstrated in a severely thrombocytopenic patient with acute myelogenous leukemia in relapse, who was supported entirely with two autologous frozen platelet transfusions for the 13 days before bone marrow activity resumed.
Dayian
G
G
Harris
H L
HL
Vlahides
G D
GD
Pert
J H
JH
D001692:Biological Transport; D001792:Blood Platelets / Q000502:physiology; D001793:Blood Preservation / Q000379:methods*; D005615:Freezing; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000628:therapy; D010974:Platelet Aggregation; D017713:Platelet Transfusion; D012701:Serotonin / Q000097:blood; D014182:Transplantation, Autologous
D012701:Serotonin