[Replication of a local strain of swine parvovirus in swine kidney cell cultures].
The kinetics of replication of strain of parvovirus in swine was investigated in the primary cell cultures of swine kidney. The morphologic changes were traced in inoculated cultures by microscopic observation and the replication of the virus in the cells by immune-fluorescent examinations. The quality of the virus in the cell monolayer and in the nutritive medium, in the different periods after the infection, was determined by hemagglutination test. By the immune-fluorescent examinations, the virus was proved still in the first hours, while the first morphologic changes of the monolayer were determined 72 hours after the infection. The nuclear fluorescent appears at the 12-th hour and increases its intensity 24 hours after the infection. At the 72-nd hour and later predominates the cytoplasmatic fluorescent. The cell monolayer produces virus up to the 96-th hour, and the virus titer reaches its maximum 120 hours after the inoculation, which is the most appropriate moment for the yield of virus.
Peev
Ia
Ia
Motovski
A
A
Aleksandrov
M
M
D000818:Animals; D003588:Cytopathogenic Effect, Viral; D005453:Fluorescence; D007700:Kinetics; D010321:Parvoviridae / Q000502:physiology*; D013552:Swine / Q000382:microbiology*; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
Replikatsiia na mesten shtam parvovirus po svinite v kletŭchni kulturi ot svinski bŭbrek.