Pluripoietin alpha: a second human hematopoietic colony-stimulating factor produced by the human bladder carcinoma cell line 5637.
We have identified a factor constitutively produced by the human bladder carcinoma cell line 5637, which has the following capacities: to induce the differentiation of the human promyelocytic leukemic cell line HL-60; to induce the expression of chemotactic peptide receptors in leukemic cells as well as in normal peripheral blood granulocytes; to function as a chemoattractant for neutrophils as well as, under certain conditions, inhibiting their migration; to support the growth of eosinophil and granulocyte/macrophage progenitors, which is maximal when scored at day 14; and to support the growth of human mixed pluripotent progenitors and erythroid bursts from normal human bone marrow. This factor has a molecular size of 32 kDa by gel filtration and 16.5 kDa by NaDodSO4/PAGE. We have termed this factor pluripoietin alpha.
Gabrilove
J L
JL
Welte
K
K
Harris
P
P
Platzer
E
E
Lu
L
L
Levi
E
E
Mertelsmann
R
R
Moore
M A
MA
D001854:Bone Marrow Cells; D002277:Carcinoma / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D002634:Chemotaxis, Leukocyte; D004920:Erythropoiesis; D006133:Growth Substances / Q000302:isolation & purification* / Q000502:physiology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000502:physiology; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000502:physiology; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000032:analysis
D006133:Growth Substances; D009363:Neoplasm Proteins; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic