title: Absorption and transport of radioactive tracers in the midgut of the malaria mosquito, Anopheles stephensi.
abstract: Three radiolabeled substances were mixed with fresh heparinized mouse blood and fed to female Anopheles stephensi through a chicken crop membrane. Resorption and transport in the anterior (A) and posterior (P, stomach) parts of the midgut were investigated by means of electron microscopic autoradiography. Digestion products of 125I-labeled bovine serum albumin (BSA) were resorbed only in the P-part, showing a biphasic pattern with maximal values 4 and 18 hr after feeding. Uptake of 3H-labeled amino acids started immediately after feeding in both midgut parts. [14C]glucose was mainly absorbed in the P-part. Labeled carbohydrate storage deposits formed during the initial phase of digestion and were mobilized around 36 hr after feeding. After feeding the mosquitoes on 125I-BSA, oocyte protein yolk spheres and abdominal cuticle became labeled. Ingestion of 3H-labeled amino acids caused the formation of silver grains over follicles and fat body lipids. Feeding on [14C]glucose resulted in labeled fat body carbohydrates and oocyte protein yolk spheres.
authors.affiliation: Swiss Tropical Institute, Basel, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Schneider
authors.forename: W
authors.initials: W
authors.lastname: Rudin
authors.forename: H
authors.initials: H
authors.lastname: Hecker
mesh_terms: D000818:Animals; D000852:Anopheles / Q000378:metabolism* / Q000648:ultrastructure; D004064:Digestive System / Q000378:metabolism / Q000648:ultrastructure; D007408:Intestinal Absorption; D007457:Iodine Radioisotopes; D008854:Microscopy, Electron; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine