The effect of antisecretory factor on the permeability of nerve cell membrane to chloride ion.
The antisecretory factor (ASF) is a hormone-like protein (m.w. 60,000) that most effectively counteracts hypersecretion in vivo in the small intestine of pigs and rats. The present report demonstrate that 10(-13) moles of ASF inhibits significantly the 36Cl- permeation through the isolated neuronal plasma membrane of Deiters' cells in rabbits. This effect was enhanced by 0.2 mM gamma-aminobutyric acid (GABA), and quenched by the addition of anti-ASF immunoglobulins; pretreatment of the neuronal membrane with nipecotic acid (10(-6) M) or with bicuculline (10(-3) M) abolished the ASF action whilst picrotoxin (10(-4) M) pretreatment left the inhibitory effect of ASF unaffected. The results suggest that ASF blocks chloride channels in neuronal membranes, including those channels activated by GABA.
Lange
S
S
Department of Clinical Bacteriology, Faculty of Medicine, University of Göteborg, Sweden.
Lönnroth
I
I
Palm
A
A
Hydén
H
H
D000818:Animals; D000930:Antidiarrheals / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002712:Chlorides / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D009479:Neuropeptides / Q000494:pharmacology*; D011817:Rabbits
D000930:Antidiarrheals; D002712:Chlorides; D009479:Neuropeptides; C049628:antisecretory factor