title: Development and regional expression of beta nerve growth factor messenger RNA and protein in the rat central nervous system.
abstract: The presence of nerve growth factor (NGF) mRNA and protein in the rat central nervous system is documented. Blot-hybridization analysis showed an abundance of NGF mRNA in the hippocampus, cerebral cortex, and olfactory bulb. Enzyme immunoassay confirmed significant levels of a NGF-like protein in the hippocampus and cerebral cortex. Bioassay of a NGF-like immunoaffinity-purified protein from these regions was physiologically indistinguishable from NGF. Immunohistochemistry revealed a widespread distribution of NGF-like reactivity in the adult brain, preferentially in fiber tracts. NGF mRNA accumulation began at birth, with adult levels reached 3 weeks postnatally. Enzyme immunoassay detected the presence of a NGF-like protein in the embryonic rat brain. Postnatally, the level of NGF-like protein reached a maximum at 3 weeks. Additionally, a distinct fetal form of NGF may exist.
authors.forename: S R
authors.initials: SR
authors.lastname: Whittemore
authors.forename: T
authors.initials: T
authors.lastname: Ebendal
authors.forename: L
authors.initials: L
authors.lastname: Lärkfors
authors.forename: L
authors.initials: L
authors.lastname: Olson
authors.forename: A
authors.initials: A
authors.lastname: Seiger
authors.forename: I
authors.initials: I
authors.lastname: Strömberg
authors.forename: H
authors.initials: H
authors.lastname: Persson
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009414:Nerve Growth Factors; D012333:RNA, Messenger