Analysis of cellular and membrane extracts of human leukocytes from rheumatoid arthritis patients using two-dimensional electrophoresis.
Peripheral blood leukocytes from 30 patients with classic or definite rheumatoid arthritis (RA) and 18 healthy controls were separated into lymphocytes, monocytes and granulocytes and labelled with 35S-methionine. The integral membrane proteins with an amphiphilic nature were separated from hydrophilic proteins by use of the nonionic detergent Triton X-114. Both whole cells and membrane proteins were isotope labelled and separated by high resolution two-dimensional electrophoresis under denaturing conditions. Polypeptide spots were visualised by autoradiography. No consistent differences were found when leukocytes from RA patients were compared to the controls.
Berner Berntzen
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Willard-Gallo
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Munthe
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Jellum
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D001172:Arthritis, Rheumatoid / Q000097:blood*; D001798:Blood Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006098:Granulocytes / Q000032:analysis; D006801:Humans; D007962:Leukocytes / Q000032:analysis*; D008214:Lymphocytes / Q000032:analysis; D008565:Membrane Proteins / Q000097:blood; D009000:Monocytes / Q000032:analysis; D017830:Octoxynol; D011092:Polyethylene Glycols
D001798:Blood Proteins; D008565:Membrane Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40