[Studies on an antibody-coated tube method of aldosterone].
We studied on the basic performance of a non-extraction coated tube RIA kit, DPC Aldosterone kit (Nippon DPC Corporation). 1. The average binding CVs of the calibrators (25 to 1,200 pg/ml) were stable in a range from 1.4 to 1.9%. 2. The minimum sensitivity was 17 pg/ml from 2SD method of 0 calibrator. 3. The intra-assay reproducibility test showed an average CV as 9.0%, and the inter-assay test showed as 8.8%. 4. The recovery ratios of a recovery test were 85.4 to 121.6%. 5. The correlation test with another maker's kit showed a correlation curve as y = 0.970 x + 19.68 and a correlation coefficiency as r = 0.970. 6. The normal range, the correlation between fasting recumbency and daytime sitting, and the correlation between walking and resting, showed similar results as former reports.
Ishihara
S
S
Radiosotope Department, Sumitomo Hospital, Osaka-shi, Japan.
Moriuma
H
H
Mishima
C
C
Honda
M
M
Tomonobu
M
M
Kanao
K
K
Usami
N
N
D000328:Adult; D000450:Aldosterone / Q000097:blood*; D000906:Antibodies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012016:Reference Values
D000906:Antibodies; D011933:Reagent Kits, Diagnostic; D000450:Aldosterone