[Expression of the surface antigen in human gastric cancer cells and the relation to cell cycles--correlated analysis with flow cytometry].
Expression of tumor-associated antigen in different gastric cancer cell lines and different phases of cell cycle was studied cytochemically. The antigen was recognized by the monoclonal antibody (McAb) PC1 against gastric cancer cells. By using the McAb PC1 as first antibody, the indirect immunofluorescence stain and the peroxidase-anti-peroxidase (PAP) stain were done on the gastric cancer cell lines (MGC 803, SGC 7901 and BGC 823). It was shown that PC1 antigen was mainly expressed on the membrane of these cells and only a certain percentage of the cells gave the positive reaction with different intensities. It was obvious that the expression of PC1 antigen was heterogeneous in nature. The heterogeneity of the PC1 antigen expression in gastric cancer cells might be due to either various subpopulations in the cell lines or different phases of cell cycle. In order to go further into the question, we studied quantitatively the expression of PC1 antigen in gastric cancer cell lines (MGC 803, STC 7901 and BGC 823) and the relationship between the antigen expression and cell cycle by double fluorescence stain and two-dimensional flow cytometry. It was found that expression levels of PC1 antigen in these cell lines were in the following order: MGC 803 greater than SGC 7901 greater than BGC 823. The PC1 antigen predominantly expressed on G1 phase for MGC 803 and G1, G2-M phase for SGC 7901 respectively. And uniform low level of PC1 antigen expression was found for BGC 823 throughout the cell cycle. Therefore, the PC1 antigen expression is dependent on cell cycle in MGC 803 and SGC 7901 cell lines.
Wei
S M
SM
Beijing Institute for Cancer Research.
Guo
Q
Q
Dong
Z W
ZW
Xue
S B
SB
Song
P G
PG
D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002453:Cell Cycle; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D013274:Stomach Neoplasms / Q000276:immunology*
D000951:Antigens, Neoplasm; D000954:Antigens, Surface