Growth of tumor colonies mediated by phorbol ester and 13-cis retinoic acid in mixed cultures.
The aim of the study was to investigate the influence of the substances modifying cell phenotype (phorbol ester and 13-cis retinoic acid) on the growth of mouse neuroblastoma N2a cells in the mixed culture system. The results were compared with monoculture system and with tumor multicellular spheroids system where neoplastic cells are cultivated in the close contact from all sides. The suppression of the pleyotypic effect of phorbol ester was observed when N2a cells were cocultured with 3T3 fibroblasts. On the contrary, the close contact of neoplastic cells to each other (spheroids) do not change the stimulatory effect of phorbol ester on tumor cells proliferation. 13-cis retinoic acid suppressed neoplastic cells growth in all cell culture systems we used.
Szaniawska
B
B
Department of Tumor Biology, Cancer Center of the Maria Skłodowska-Curie Institute, Warsaw.
Przybyszewska
M
M
Janik
P
P
Miłoszewska
J
J
D000818:Animals; D002450:Cell Communication; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy / Q000473:pathology; D009447:Neuroblastoma / Q000188:drug therapy / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate