title: Ectopic hematopoiesis in peritoneal tumor nodules induced by the myeloproliferative sarcoma virus in DBA/2 mice.
abstract: Tumor nodules composed of fibroblasts, large undifferentiated cells, granulocytes, and small lymphocytes develop in the spleens of adult DBA/2 mice infected with the myeloproliferative sarcoma virus (MPSV). They spread thereafter in the organism, and at the terminal stage of the disease they are especially numerous on the peritoneal membrane. The present study, performed on those tumor nodules to avoid contamination by exogenous hematopoietic cells, demonstrated that they were sites of granulopoiesis, which may have occurred via the local differentiation of granulomacrophage precursor cells (GM-CFC) and perhaps also from pluripotent hematopoietic stem cells, since these two populations were present in the tumor nodules (25 +/- 11 and 13 +/- 10, respectively, per 5-10(5) cells). Almost all (88%) those GM-CFC were able to clone in vitro without added colony-stimulating factor. A comparative study with the Moloney murine sarcoma virus-induced tumor indicated that the local production of hematopoiesis-stimulating factors was not sufficient to allow such ectopic granulopoiesis. These results imply the presence of a specific hematopoietic microenvironment in the MPSV-induced tumor nodules.
authors.forename: F
authors.initials: F
authors.lastname: Smadja-Joffe
authors.forename: M C
authors.initials: MC
authors.lastname: Le Bousse-Kerdiles
authors.forename: A M
authors.initials: AM
authors.lastname: Bertoli
authors.forename: V
authors.initials: V
authors.lastname: Degiorgis
authors.forename: B
authors.initials: B
authors.lastname: Caillou
authors.forename: C
authors.initials: C
authors.lastname: Jasmin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D003114:Colony-Forming Units Assay; D003470:Culture Media; D005347:Fibroblasts / Q000503:physiopathology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000503:physiopathology*; D007840:Latex; D008264:Macrophages / Q000503:physiopathology; D051379:Mice; D008811:Mice, Inbred DBA; D008854:Microscopy, Electron; D008980:Moloney murine sarcoma virus; D009196:Myeloproliferative Disorders / Q000503:physiopathology*; D014411:Neoplastic Stem Cells / Q000503:physiopathology; D010534:Peritoneal Neoplasms / Q000503:physiopathology*; D010587:Phagocytosis; D014412:Tumor Virus Infections / Q000503:physiopathology*
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D007840:Latex