title: Cryopreservation of infective-stage larvae of Brugia malayi.
abstract: Third-stage larvae of Brugia malayi (Guizhou, China strain), in RPMI-1640, 6% DMSO and 15% calf serum, frozen slowly in the vapor phase of liquid nitrogen prior to emersion in liquid nitrogen, remained viable for as long as as 321 days and were infective to Mongolian jirds. All DMSO should be removed from the freezing medium to restore larval activity after freezing.
authors.affiliation: Guizhou Provincal Institute of Parasitic Diseases, Guiyang, People's Republic of China.
authors.forename: S H
authors.initials: SH
authors.lastname: Wang
authors.forename: H J
authors.initials: HJ
authors.lastname: Zheng
mesh_terms: D000330:Aedes; D000818:Animals; D002009:Brugia / Q000254:growth & development* / Q000502:physiology; D005260:Female; D005615:Freezing; D005849:Gerbillinae; D007814:Larva / Q000254:growth & development / Q000502:physiology; D011309:Preservation, Biological