Antigenic phenotype of myelomonocytic progenitors (CFU-GM) in chronic myeloproliferative disorders.
The antigenic phenotype of myelomonocytic progenitors [colony-forming unit granulocyte-macrophage (CFU-GM)] from 33 patients with chronic myeloproliferative disorders was investigated using four cytotoxic monoclonal antibodies. Monoclonal antibodies S3-13, S8-6, and S16-144 which recognize normal hemopoietic progenitors of different lineages reacted with almost all CFU-GM. R1B-19 monoclonal antibody identified two subpopulations of myelomonocytic progenitors (type 1 and 2 CFU-GM), as reported previously in normal subjects. In 3 of 11 patients with chronic myelogenous leukemia, in 1 of 2 patients with chronic myelomonocytic leukemia, and in 2 of 4 patients with polycythemia vera, a higher proportion of the more immature CFU-GM (type 1) was detected in bone marrow cells. The more differentiated CFU-GM (type 2) is not detectable in normal peripheral blood. By contrast, in 14 of 15 chronic myelogenous leukemia patients, in 1 of 2 chronic myelomonocytic leukemia patients and in 3 of 8 patients with idiopathic myelofibrosis, it was present in high to very high proportions. It is clear from these findings that the antigens present on normal CFU-GM are expressed in chronic myeloproliferative disorders. The proportion and distribution of type 1 and 2 CFU-GM, on the other hand, are very different from those observed in the normal subjects.
Ferrero
D
D
Tarella
C
C
Pregno
P
P
Pileri
A
A
Gallo
E
E
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D009196:Myeloproliferative Disorders / Q000276:immunology* / Q000473:pathology; D011087:Polycythemia Vera / Q000276:immunology / Q000473:pathology; D055728:Primary Myelofibrosis / Q000276:immunology / Q000473:pathology; D013920:Thrombocythemia, Essential / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface