Cell growth and hemoglobin synthesis in murine erythroleukemic cells propagated in high density microcapsule culture.
A new microencapsulation technology, developed for the encapsulation of living cells, has been demonstrated to be useful for the study of growth and differential gene expression using Friend erythroleukemic cells cultured at high cell densities. Using this technology, cultures of FL Clone 745 cells were encapsulated within semipermeable membranes composed of cross-linked alginic acid and poly-l-lysine. Cell growth studies measuring total cell number demonstrated an average generation time of 8.5 h in 5% (vol/vol) microcapsule cultures vs. 8.0 h in suspension cultures. Similar microcapsule cultures were serially propagated for more than 90 cell generations (13 sequential passages) with no significant change in this growth rate. In addition, final culture densities of greater than 1.0 X 10(8) cells/ml of intracapsular volume were attained using a 3% (vol/vol) microcapsule culture in conjunction with a standard refeeding schedule. Comparison of the level of dimethyl sulfoxide-induced hemoglobin production in suspension and microcapsule cultures demonstrated that the total amount of hemoglobin produced on a per cell basis was comparable in both systems. Due to the retention characteristics of the semipermeable membrane, the concentration of detergent-released hemoglobin, relative to other released protein, was approximately twofold higher in microcapsule cultures than in control suspension cultures.
Jarvis
A P
AP
Grdina
T A
TA
Sullivan
M F
MF
D000464:Alginates; D000818:Animals; D001672:Biocompatible Materials; D002214:Capsules; D002455:Cell Division; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005622:Friend murine leukemia virus; D020723:Glucuronic Acid; D006454:Hemoglobins / Q000096:biosynthesis*; D006603:Hexuronic Acids; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D008567:Membranes, Artificial; D051379:Mice; D011107:Polylysine
D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D006454:Hemoglobins; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D011107:Polylysine; D020723:Glucuronic Acid; D004121:Dimethyl Sulfoxide