Partial purification of a nuclear protein that binds to the CCAAT box of the mouse alpha 1-globin gene.
We enriched a fraction from nuclear extracts of murine erythroleukemia cells which contains a protein able to form stable complexes with the promoter region of the alpha 1-globin gene. Binding activity, which is present in mouse brain and a variety of cultured mouse and human cell lines, is not erythroid cell specific. Binding studies with alpha-globin gene promoter deletion mutants as well as DNase I footprinting and dimethyl sulfate protection studies showed that the factor bound specifically to the CCAAT box of the alpha 1 promoter. Enriched factor preparations exhibited weak binding to the promoter region of the beta maj-globin gene. This suggests that this protein could bind differentially to these two promoters in vivo. The enriched factor may be a ubiquitous nuclear protein involved in the differential regulation of the alpha 1- and beta maj-globin genes.
Cohen
R B
RB
Sheffery
M
M
Kim
C G
CG
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005796:Genes; D005914:Globins / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute; D007942:Leukemia, Experimental; D051379:Mice; D009154:Mutation; D009698:Nucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D011401:Promoter Regions, Genetic
D004268:DNA-Binding Proteins; D009698:Nucleoproteins; D005914:Globins