title: Effect of small bowel resection on the intestinal surface acid microclimate in the rat.
abstract: The effect of extensive (65%) proximal and middle small bowel resection on the intestinal surface acid microclimate (ISAM) of the remaining ileum in the rat was examined and the results were compared to those of sham-operated rats. ISAM pH measurements were performed in vivo using a pH microelectrode; incubation was performed in Krebs-Ringer phosphate buffer (pH 7.40 +/- 0.02). In the resected rats, ISAM pH of 6.03 +/- 0.07 and 7.22 +/- 0.03 were recorded in the proximal and distal part of the remaining ileum, respectively. In the sham-operated rats, ISAM pH of 6.04 +/- 0.07, 6.98 +/- 0.03 and 7.28 +/- 0.02 were recorded in the proximal jejunum and in the proximal and distal part of the corresponding ileal segment. ISAM pH was significantly lower (p less than 0.01) in the proximal part of the remaining ileum of the resected rats as compared to the corresponding part in the sham-operated rats but was similar in distal ileum. In fact, the ISAM pH of the proximal part of the remaining ileum of resected rats was as acidic as that of the jejunum of the sham-operated rats. These results clearly demonstrate that adaptation in the ISAM occurs in the remaining ileum following extensive resection of proximal and middle small intestine.
authors.affiliation: Department of Pediatric Gastroenterology, Vanderbilt University School of Medicine, Nashville, Tenn.
authors.forename: H M
authors.initials: HM
authors.lastname: Said
authors.forename: W
authors.initials: W
authors.lastname: Tipton
authors.forename: W
authors.initials: W
authors.lastname: Nylander
authors.forename: E
authors.initials: E
authors.lastname: Urban
mesh_terms: D000818:Animals; D006863:Hydrogen-Ion Concentration; D007082:Ileum; D007421:Intestine, Small / Q000601:surgery*; D007583:Jejunum; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013499:Surface Properties