The staining of type V collagen obtained from FBJ virus-induced osteosarcoma with concanavalin A.
Type V collagen from FBJ virus-induced osteosarcoma of mice has a high content of saccharide as has been noted for type V collagens from different sources. In the present study, this collagen was found to contain significant amounts of mannose and hexosamine. Three alpha chains of this collagen were electrophoretically separated and cleaved by cyanogen bromide. The cyanogen bromide peptides, following their separation by SDS-polyacrylamide gel electrophoresis, were transferred onto nitrocellulose paper and stained with concanavalin A. Several bands derived only from alpha 3(V) stained positively, but this was inhibited by the presence of alpha-methylmannoside. Thus, at least one of these three chains appears to have an asparagine-linked oligosaccharide.
Yamagata
S
S
Yamagata
T
T
D000818:Animals; D001216:Asparagine / Q000032:analysis; D003094:Collagen / Q000032:analysis*; D003208:Concanavalin A / Q000378:metabolism*; D003488:Cyanogen Bromide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006595:Hexosamines / Q000032:analysis; D008358:Mannose / Q000032:analysis; D012516:Osteosarcoma / Q000032:analysis* / Q000382:microbiology; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats
D006595:Hexosamines; D010446:Peptide Fragments; D003208:Concanavalin A; D001216:Asparagine; D003094:Collagen; D003488:Cyanogen Bromide; D008358:Mannose