Chemical modification of histidine, tyrosine, tryptophan and cysteine residues in carp (Cyprinus carpio) muscle enolase.
Enolase from carp (Cyprinus Carpio) muscle was modified by diethylpyrocarbonate, tetranitromethane, N-bromosuccinimide and 5,5'-dithiobis(2-nitrobenzoic acid). The extent and rate of modification and its effect on the enzyme activity were determined. Modification of histidine, tyrosine and tryptophan residues caused complete inactivation of the enzyme; Mg2+ as well as 2-phosphoglycerate markedly altered the rates of modification and inactivation. The above-mentioned amino acid residues seem to be essential for the functioning of muscle enolases. Modification of cysteine residues had no effect on the enolase activity.
Pietkiewicz
J
J
Department of Biochemistry, Medical School, Wrocław, Poland.
Kustrzeba-Wójcicka
I
I
Wolna
E
E
Wolny
M
M
D000596:Amino Acids / Q000502:physiology*; D000818:Animals; D001974:Bromosuccinimide / Q000494:pharmacology; D002347:Carps / Q000378:metabolism*; D003530:Cyprinidae / Q000378:metabolism*; D003545:Cysteine / Q000502:physiology; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D006639:Histidine / Q000502:physiology; D009132:Muscles / Q000201:enzymology*; D009584:Nitrogen / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000037:antagonists & inhibitors*; D013774:Tetranitromethane / Q000494:pharmacology; D014364:Tryptophan / Q000502:physiology; D014443:Tyrosine / Q000502:physiology
D000596:Amino Acids; D014443:Tyrosine; D006639:Histidine; D014364:Tryptophan; D010751:Phosphopyruvate Hydratase; D013774:Tetranitromethane; D003545:Cysteine; D001974:Bromosuccinimide; D004047:Diethyl Pyrocarbonate; D009584:Nitrogen