A non-linear regression program in BASIC for estimating Km and Vmax.
This paper gives a program in BASIC for calculating the kinetic parameters Km and Vmax for an enzyme reaction from a set of paired values of reaction velocity at given substrate concentrations. An initial estimate of the two parameters is made using a weighted linear regression, these values are then used in an iterative process to fit the data to the Michaelis-Menten equation and give final values of Km and Vmax with their associated standard errors.
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Department of Biological Sciences, Sheffield City Polytechnic, UK.
D000465:Algorithms; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008432:Mathematical Computing; D008954:Models, Biological; D011381:Programming Languages; D012044:Regression Analysis; D012984:Software
D004798:Enzymes