title: The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants.
abstract: The chain specificities of 18 Ak and 26 Ab-reactive anti-Ia monoclonal antibodies have been determined. L cells were transfected with haplotype-matched (A alpha k:A beta k, A alpha b:A beta k) or haplotype-mismatched (A alpha k:A beta b, A alpha b:A beta k) cDNA pairs, lines expressing high levels of surface A complex were selected, and antibody reactivity with a panel of reagents was assessed by cytofluorimetric analysis. Most of the antibodies recognized a determinant specified by one chain, either alpha or (more commonly) beta. A few examples of more complex determinants were also observed. A knowledge of the chain specificities of anti-Ia monoclonal antibodies should prove useful for a variety of studies aimed at dissecting Ia structure-function relationships.
authors.forename: D
authors.initials: D
authors.lastname: Landias
authors.forename: B N
authors.initials: BN
authors.lastname: Beck
authors.forename: J M
authors.initials: JM
authors.lastname: Buerstedde
authors.forename: S
authors.initials: S
authors.lastname: Degraw
authors.forename: D
authors.initials: D
authors.lastname: Klein
authors.forename: N
authors.initials: N
authors.lastname: Koch
authors.forename: D
authors.initials: D
authors.lastname: Murphy
authors.forename: M
authors.initials: M
authors.lastname: Pierres
authors.forename: T
authors.initials: T
authors.lastname: Tada
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007739:L Cells; D046911:Macromolecular Substances; D051379:Mice; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances