Cocompartmentation of proteins and K+ within the living cell.
Monolayer H-50 tissue culture cells were treated with Triton X-100 and Brij 58 nonionic detergents, and their electron microscopic morphology along with the release of the intracellular proteins and K+ were studied. Although Triton X-100 was more effective, both detergents removed the lipoid membranes within 5 min. The mobilization and solubilization of the cytoplasmic and nuclear proteins occurred much faster with Triton X-100 than with Brij 58. In Triton X-100-treated cells, the loss of K+ was complete within 2 min. The loss of K+ from the Brij 58-treated cells was complete only after 10 min and the mobilization of K+ showed sigmoid-type release kinetics. These results support the view that most of K+ and "diffusible" proteins not freely dissolved in the cellular water, but they are cocompartmentalized inside the living cell.
Kellermayer
M
M
Ludany
A
A
Jobst
K
K
Szucs
G
G
Trombitas
K
K
Hazlewood
C F
CF
D000818:Animals; D002451:Cell Compartmentation; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis / Q000187:drug effects; D002592:Cetomacrogol; D002522:Chlorocebus aethiops; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D017830:Octoxynol; D011092:Polyethylene Glycols; D011188:Potassium / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility
D011506:Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; D002592:Cetomacrogol; D011188:Potassium