Chromosomal localization of the human apolipoprotein B gene and detection of homologous RNA in monkey intestine.
A cDNA clone of the human apolipoprotein B-100 was used as a hybridization probe to detect homologous sequences in both flow-sorted and in situ metaphase chromosomes. The results indicate that the gene encoding this protein is on the distal end of the short arm of chromosome 2 (2p23-2p24). RNA isolated from monkey small intestine contained sequences (6.5 and 18 kilobases) homologous to the cDNA of apolipoprotein B-100. These results are consistent with the hypothesis that one gene codes for both the intestinal (B-48) and the hepatic (B-100) forms.
Deeb
S S
SS
Disteche
C
C
Motulsky
A G
AG
Lebo
R V
RV
Kan
Y W
YW
D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3; D006801:Humans; D007422:Intestines / Q000502:physiology; D008254:Macaca nemestrina; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics
D001055:Apolipoproteins B; D012333:RNA, Messenger