Three-step procedure for isolation of epithelial cells from the lungs of sheep with jaagsiekte.
An efficient and reproducible technique is described for the isolation of transformed sheep pulmonary adenomatosis cells. It includes three basic steps: prolonged trypsinisation to kill fibroblasts, magnetic removal of macrophages and adherence to remove the rapidly adherent cells. The resultant preparations of lung cells were enriched to 96.6 per cent type 2 pneumocytes.
Jassim
F A
FA
Moredun Research Institute, Edinburgh.
Sharp
J M
JM
Marinello
P D
PD
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004848:Epithelium / Q000473:pathology; D008168:Lung / Q000473:pathology*; D011648:Pulmonary Adenomatosis, Ovine / Q000473:pathology*; D012756:Sheep