title: Quantitative study of liver metastases from colon cancer in rats after treatment with cyclosporine A.
abstract: Liver metastases were produced in syngeneic BD IX rats by intraportal injection of colon cancer cell aggregates. The cells originated from the DHD/K12 cell line, derived from a 1,2-dimethylhydrazine (CAS: 540-73-8)-induced colon adenocarcinoma in BD IX rats. The animals received either cyclosporine A (CSA) or the excipients alone (control) through daily gastric intubation during 6 weeks. Multiple and very large hepatic metastases were observed early in 100% of the CSA-treated rats. The mean tumor volume was approximately 2,000 times higher in the CSA-treated group than in the controls (P less than .01). Survival time in the CSA-treated group was shortened (P less than .01) by generalized metastatic disease. Easy production of metastasis from colon cancer in 100% of the animals and precise estimation of tumor volume may prove useful for future therapeutic studies of secondary hepatic disease.
authors.forename: J
authors.initials: J
authors.lastname: van der Elst
authors.forename: J
authors.initials: J
authors.lastname: De Greve
authors.forename: F
authors.initials: F
authors.lastname: Geerts
authors.forename: W
authors.initials: W
authors.lastname: De Neve
authors.forename: G
authors.initials: G
authors.lastname: Storme
authors.forename: G
authors.initials: G
authors.lastname: Willems
mesh_terms: D000230:Adenocarcinoma / Q000517:prevention & control / Q000556:secondary*; D000818:Animals; D001835:Body Weight; D002460:Cell Line; D003110:Colonic Neoplasms; D003201:Computers; D003524:Cyclosporins / Q000627:therapeutic use*; D008113:Liver Neoplasms / Q000517:prevention & control / Q000556:secondary*; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D009929:Organ Size; D010534:Peritoneal Neoplasms / Q000556:secondary; D011169:Portal Vein; D051381:Rats; D013997:Time Factors
chemical_list: D003524:Cyclosporins