Biochemical analyses of murine erythropoietin from plasma and from cloned erythroleukemia cells.
The hydrophobicity, ionic charges, degrees of glycosylation, and Western blot patterns of murine plasma erythropoietin and erythropoietin produced by clones of two murine erythroleukemic cell lines (IW32 and NN10) were compared. Erythropoietins from these different sources exhibited a number of similarities in their biochemical properties: identical retention on DEAE Affigel blue column and similar apparent molecular weights (30-36 kDa) in Western blot analysis. However, differences were also observed: heterogeneous binding to different lectin columns and varied retention on a phenyl Sepharose column. The data thus confirm that these murine plasma erythropoietins have biochemical properties in common with previously studied erythropoietins from humans or sheep. The major difference between erythropoietins secreted by erythroleukemic cell lines and other molecules belonging to the same family appears to be related to their glycosylation.
Choppin
J
J
Egrié
J
J
Casadevall
N
N
Lacombe
C
C
Muller
O
O
Varet
B
B
D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002999:Clone Cells / Q000378:metabolism; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004921:Erythropoietin / Q000097:blood*; D037102:Lectins; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D010209:Paper
D037102:Lectins; D004921:Erythropoietin; D003101:Collodion