Actinomyces viscosus colonization of mouse teeth.
The implantation of Actinomyces viscosus strain T14V-J1 was studied in mice in vivo. The optimum age range for implantation was from 40 to 80 days. The minimum infective dose of strain T14V-J1 required for colonization in all inoculated animals (ID100) was 5 X 10(8) CFU for 40-day-old mice and 1 X 10(9) CFU for 100-day-old mice. The high ID100 required for implantation of strain T14V-J1 in these mice is consistent with the finding that tooth surfaces of these animals are not colonized by detectable indigenous Actinomyces. The infection could also be transmitted to non-infected mice housed with infected mice. This in vivo model can be used to evaluate the potential for reducing or preventing Actinomyces colonization on teeth by vaccination with fimbrial adhesins.
Crawford
P C
PC
Clark
W B
WB
D000190:Actinomyces / Q000302:isolation & purification / Q000502:physiology*; D000327:Adsorption; D000818:Animals; D004463:Ecology; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D012463:Saliva / Q000502:physiology; D013997:Time Factors; D014070:Tooth / Q000382:microbiology*