[Internucleosome interactions: detecting the dinucleosome fragmentation of chromatin using micrococcal nuclease. Heterogeneity of nucleosomes and localization of histone H1].
The content of histone H1 (H1/H4 ratio) in dinucleosomes with the DNA of various length liberated from L-cell nuclear chromatin by micrococcal nuclease was analyzed. It was found that the histone H1 content in the dichromatosome is two times as low as that in the largest dinucleosome and in the complete mononucleosome. The set of chromatin fragments liberated from the Triton X-100 pretreated nuclei differs considerably from that of chromatin sites devoid of histone H1 (the de novo replicating chromatin and the chromatin formed on the undermethylated DNA). A scheme for asymmetric distribution of histone H1 with molecules oriented along the nucleosomal fibril, which reflects the peculiarities of chromatin fragmentation by micrococcal nuclease with predominant liberation of the dichromatosome, is proposed.
Kir'ianov
G I
GI
Smirnova
T A
TA
Manamsh'ian
T A
TA
Khodosovskaia
A M
AM
D000818:Animals; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis*; D007739:L Cells; D046911:Macromolecular Substances; D051379:Mice; D008836:Micrococcal Nuclease; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000032:analysis*; D051381:Rats
D002843:Chromatin; D006657:Histones; D046911:Macromolecular Substances; D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease
Mezhnukleosomnoe vzaimodeĭstvie: vyiavlenie dinukleosomnoĭ fragmentatsii khromatina mikrokokkovoĭ nukleazoĭ. Geterogennost' dinukleosom i lokalizatsiia gistona H1.