Conversion of urea or ammonia into essential amino acids (L-leucine, L-valine, and L-isoleucine) using multienzyme systems and NADH-dextran immobilised in artificial cells.
Artificial cells containing leucine dehydrogenase (EC 1.4.1.9), alcohol dehydrogenase (EC 1.1.1.1; or glucose dehydrogenase, EC 1.1.1.47; or lactic dehydrogenase, EC 1.1.1.27; or malic dehydrogenase, EC 1.1.1.37), urease (EC 3.5.1.5) and dextran-NAD+ were prepared. Ammonia or urea could be converted into L-leucine, L-valine and L-isoleucine using artificial cells with four different multienzyme systems.
Gu
K F
KF
Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
Chang
T M
TM
D000601:Amino Acids, Essential / Q000096:biosynthesis*; D000641:Ammonia / Q000378:metabolism*; D002214:Capsules; D002477:Cells; D004800:Enzymes, Immobilized; D066298:In Vitro Techniques; D008107:Liver Diseases / Q000378:metabolism / Q000628:therapy; D008567:Membranes, Artificial; D009097:Multienzyme Complexes; D014508:Urea / Q000378:metabolism*
D000601:Amino Acids, Essential; D002214:Capsules; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009097:Multienzyme Complexes; D000641:Ammonia; D014508:Urea