title: [Molecular composition of the fractions of immunoglobulin preparations studied by gel filtration].
abstract: The method of gel filtration in columns permitted the separation of aggregated fractions into polymers whose content did not exceed 10% and dimers, their content ranging from 3.6% to 22.11%. The preparations were also found to contain fractions of monomers and fragments, Fab-fragments being detected in 10 out of 20 batches under study (4.04-27.36%). The shelf life of all preparations did not exceed 8-12 months. The use of spectrophotometric techniques ensured obtaining the most objective results in the calculation of the percentage of fractions contained in immunoglobulin preparations. The evaluation of the molecular composition of immunoglobulin preparations by the method of gel filtration is conducive to the improvement of their quality.
authors.forename: T M
authors.initials: TM
authors.lastname: Kargina
authors.forename: V F
authors.initials: VF
authors.lastname: Runova
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Gavrilenkova
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D004356:Drug Storage; D006801:Humans; D007128:Immunoglobulin Fragments / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis* / Q000592:standards; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G
vernacular_title: Izuchenie molekuliarnogo sostava fraktsiĭ immunoglobulinovykh preparatov metodom gel'-fil'tratsii.