Phenol sulfotransferase in human lung.
Human lung phenol sulfotransferase was about 1000-fold purified using the earlier described procedure. 2-Naphthol, p-nitrophenol, phenol, salicylamide, p-methylphenol, o-methoxyphenol, adrenaline, and dopamine were tested as substrates for human lung PST using the barium hydroxide procedure and the ECTEOLA-cellulose method. Km values for sulfate donor (PAPS) and for different sulfate acceptors were determined. 2,6-Dichloro-4-nitrophenol was found to be a competitive inhibitor of human lung PST with Ki = 8.87 +/- 0.08 microM. High salt concentration and Mg2+, Mn2+, and Zn2+ inhibited lung PST. The molecular weight of human lung PST was estimated as 38,000 and 35,000 by gel filtration and SDS-gel electrophoresis, respectively.
Barańczyk-Kuźma
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D000328:Adult; D015239:Arylsulfotransferase; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D005590:Fractional Precipitation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008168:Lung / Q000201:enzymology*; D008875:Middle Aged; D008970:Molecular Weight; D009596:Nitrophenols / Q000494:pharmacology; D013379:Substrate Specificity; D013466:Sulfurtransferases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*
D009596:Nitrophenols; C015802:2,6-dichloro-4-nitrophenol; D013466:Sulfurtransferases; D015239:Arylsulfotransferase