Modulation of granule cell migration by a glia-derived protein.
Cultured explants from early postnatal mouse cerebellum were used to examine the influence of a 43-kDa glia-derived neurite-promoting factor (GdNPF) on the migration of [3H]thymidine-labeled granule cell neurons. GdNPF, which is a potent serine protease inhibitor, significantly reduced the extent of granule cell migration in a dose-dependent manner. This effect could be neutralized by addition of thrombin, which binds GdNPF. Other protease inhibitors such as aprotinin, hirudin, soybean trypsin inhibitor, leupeptin, 6-aminocaproic acid, and D-Phe-Pro-ArgCH2Cl do not show this inhibitory effect. These results demonstrate that a glia-derived protein can regulate the migration of postmitotic neurons, an important cellular event in the development of the nervous system.
Lindner
J
J
Guenther
J
J
Nick
H
H
Zinser
G
G
Antonicek
H
H
Schachner
M
M
Monard
D
D
D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D011480:Protease Inhibitors / Q000502:physiology*
D009419:Nerve Tissue Proteins; D011480:Protease Inhibitors