Soybean seed lectin gene and flanking nonseed protein genes are developmentally regulated in transformed tobacco plants.
We introduced a 17.1-kilobase soybean DNA fragment containing the lectin gene and at least four nonseed protein genes into the tobacco genome. As in soybean plants, lectin mRNA is present in tobacco seeds, accumulates and decays during tobacco seed development, and is translated into a protein that accumulates prior to dormancy. Each soybean nonseed protein mRNA is present in tobacco leaves, roots, stems, and seeds at levels similar to that found in soybean plants. We conclude that a differentially expressed soybean gene cluster is correctly regulated in transformed tobacco plants and that sequences controlling their expression are recognized by regulatory factors present in tobacco cells.
Okamuro
J K
JK
Jofuku
K D
KD
Goldberg
R B
RB
D005786:Gene Expression Regulation; D037102:Lectins / Q000032:analysis / Q000235:genetics*; D037121:Plant Lectins; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000032:analysis; D013025:Glycine max / Q000235:genetics; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D012333:RNA, Messenger