Protein synthesis in cell-free reticulocyte lysates on multi-hour incubation.
Cell-free protein synthesis in rabbit reticulocyte lysate translation mixtures has been studied during multi-hour incubations. In an impaired lysate obtained from cells stored at 0 degrees C before lysis, and showing a low initial rate of synthesis, translation could be stimulated by a factor of 4 by including RNase inhibitor and additional ATP and GTP. In translation mixtures prepared from normal lysates, protein synthesis could be improved by approximately 50% by the addition of excess GTP. The observed increases in protein synthesis were obtained by improved maintenance of the initial rate of synthesis.
Findeis
M A
MA
Department of Chemistry, Harvard University, Cambridge, MA 02138.
Whitesides
G M
GM
D000818:Animals; D002474:Cell-Free System; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008722:Methods; D014176:Protein Biosynthesis; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D007930:Leucine