Human cysteine-proteinase inhibitors: nucleotide sequence analysis of three members of the cystatin gene family.
Three genes from the human cystatin gene family of cysteine-proteinase inhibitors have been isolated from a bacteriophage lambda library containing HindIII digests of human genomic DNA. Two of the genes code for salivary cystatin SN and SA, the third is a pseudogene. The cloned genes were identified with a probe made from a salivary cystatin cDNA. The complete nucleotide sequence of the gene that codes for the precursor form of the neutral salivary protein, cystatin SN, was determined. The gene, which we name CST1, contains three exons and two intervening sequences. The expected CAT and ATA boxes are present in the 5'-flanking region of the gene. Partial nucleotide sequence determination of a second gene revealed that it codes for the precursor form of the acidic salivary protein, cystatin SA. This gene, which we name CST2, has the same gene organization as CST1. The complete nucleotide sequence of a third gene was determined. It does not contain a typical ATA box, and in addition, a premature stop codon and a frameshift deletion mutation occur within the gene. These inactivation mutations show that this gene, which we name CSTP1, is a cystatin pseudogene. These data combined with our genomic Southern-blot analyses show that the cystatin genes form a multigene family with at least seven members.
Saitoh
E
E
Laboratory of Genetics, University of Wisconsin-Madison 53706.
Kim
H S
HS
Smithies
O
O
Maeda
N
N
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D055316:Cystatin C; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D004247:DNA; D006801:Humans; D007120:Immunochemistry; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D011480:Protease Inhibitors / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger; D013363:Submandibular Gland / Q000032:analysis
C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors; D011506:Proteins; D012333:RNA, Messenger; D004247:DNA