Metabolism of prostaglandin PG-F2 alpha by freshly isolated alveolar type II cells from lungs of adult male or pregnant rabbits.
Prostaglandin F2 alpha (PG-F2 alpha) was metabolized to different products by freshly isolated alveolar type II cells from adult male or pregnant rabbits. The type II cells from the adult male rabbits metabolized PG-F2 alpha to products which co-chromatographed on HPLC with 15-keto PG-F2 alpha and 13,14-dihydro-15-keto PG-F2 alpha, metabolites of cytosolic metabolism. The cells from the pregnant rabbit metabolized the prostaglandin to several polar metabolites. The major peak co-eluted with 20-hydroxy-PG-F2 alpha, a product dependent on cytochrome P-450 metabolism. The other polar metabolites were likely secondary oxidation products, formed by both the cytosolic 15-dehydrogenase and/or the 13,14-reductase and the microsomal omega-hydroxylase. No metabolism of PG-F2 alpha was observed in fractions of alveolar macrophages or tracheal cells of either adult male or pregnant rabbits. Fractions enriched in Clara cells (40-60% purity) showed little and variable metabolism of PG-F2 alpha, qualitatively similar to that observed with type II cells. However, data was inconclusive due to the low Clara cell purity and low activity.
Devereux
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Fouts
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Eling
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D000818:Animals; D015237:Dinoprost; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D011460:Prostaglandins F / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D011817:Rabbits; D014132:Trachea / Q000378:metabolism
D011460:Prostaglandins F; D015237:Dinoprost