title: [3H] (-)sulpiride binding in rat striatum, cortex and anterior pituitary: an improved assay.
abstract: The interaction of [3H] (-)sulpiride with D2 dopamine (DA) receptors in the striatum, anterior pituitary and medial-prefrontal cortex was studied in rats, using an improved [3H] (-)sulpiride radioreceptor binding technique. Incubation on ice and a fast filtration resulted in higher specific binding (approximately 85% of total binding) a lower affinity constant (about 3 nM) and higher Bmax than reported with previous procedures. Pharmacological interactions confirmed the high selectivity of [3H] (-)sulpiride for D2 DA receptors.
authors.affiliation: Department of Neuroscience, School of Medicine, University of Cagliari, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Stefanini
authors.forename: A M
authors.initials: AM
authors.lastname: Ortu
authors.forename: F
authors.initials: F
authors.lastname: Vernaleone
authors.forename: G L
authors.initials: GL
authors.lastname: Gessa
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013469:Sulpiride / Q000378:metabolism*
chemical_list: D013469:Sulpiride