Kinetically controlled enzyme-catalyzed synthesis of kyotorphin. An optimization study.
Regarding the alpha-chymotrypsin-catalyzed synthesis of Z-Tyr-Arg-NH2, the effect on the reaction yield of the following experimental factors is discussed: DMF/buffer proportions, reaction temperature, and donor/nucleophile ratios. The experimental design shows that relatively better yields are obtained by increasing the reaction temperature and lowering the cosolvent proportion while maintaining a slight excess of nucleophile. The ascensional line reaches an optimal response surface very flat voided of a well-defined maximum.
Clapés
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P
Biological Organic Chemistry Department, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
Valencia
G
G
Reig
F
F
García Antón
J M
JM
Mata
J
J
D000700:Analgesics / Q000138:chemical synthesis*; D002918:Chymotrypsin / Q000378:metabolism*; D004723:Endorphins / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D007700:Kinetics; D013816:Thermodynamics
D000700:Analgesics; D004723:Endorphins; D007202:Indicators and Reagents; C019448:kyotorphin; D002918:Chymotrypsin