title: Studies on the organic polyanionic constituents of human acquired dental pellicle.
abstract: Extracts of acquired dental pellicle were obtained by a two-step procedure. Pellicle formed on cleaned teeth was first extracted using 2 M CaCl2, and the remainder then removed by scraping followed by solubilization in 5 per cent (w/v) EDTA. The 2 M CaCl2 extract contained high-molecular-weight glycoprotein which was present in only trace amounts in the EDTA extract. Cellulose-acetate electrophoresis of both extracts revealed low-molecular weight protein which contained phosphate. This protein tentatively identified as a phosphoprotein, disappeared as the pellicle matured. The findings have implications in relation to early bacterial colonization of the tooth surface.
authors.forename: G
authors.initials: G
authors.lastname: Embery
authors.forename: T G
authors.initials: TG
authors.lastname: Heaney
authors.forename: J B
authors.initials: JB
authors.lastname: Stanbury
mesh_terms: D003741:Dental Deposits / Q000032:analysis*; D044622:Dental Pellicle; D004588:Electrophoresis, Cellulose Acetate; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis
chemical_list: D006023:Glycoproteins; D010750:Phosphoproteins