Studies in vitro on the effects of rhein on the chemotaxis of human leukocytes.
Rhein (R: 1,8-dihydroxy-3-carboxyanthraquinone) is the active metabolite of the drug diacetylrhein (DAR), an anthraquinone molecule which has recently been proposed for the long-term treatment of osteoarthrosis. Its action mechanism in rheumatic pathology has not been fully explained. It is known that DAR, while not inhibiting the formation of prostaglandins, inhibits certain proteolytic enzymes, and acts on phlogistic cells by lysosomal enzymic and superoxide-anion modifications. Moreover DAR modifies phagocytic functions and the motility of cells. This paper is a contribution to the clarification of the last point, namely the effect of rhein on cell motility. It reports that in vitro no effect of R on random migration was found, but instead a double inhibiting effect on chemotaxis (i.e. a low-dosage and a high-dosage effect). Furthermore, R did not modify the inhibition or induce modification of chemotaxis by vinblastine. Finally R cancelled the stimulating effect of ionic potassium. The results thus indicate that R acts on the chemotaxis of the leukocytes with a complex action at different doses. The action mechanism is probably due to a membrane effect, since rhein (R) did not modify the chemotaxis-inhibiting activity of vinblastine but did interfere with the stimulating effect of K+.
Mian
M
M
Institute of Pharmacology, University of Pisa, Italy.
Azzara'
A
A
Benetti
D
D
Ruocco
L
L
Bertelli
A
A
Ambrogi
F
F
D000880:Anthraquinones / Q000494:pharmacology*; D002464:Cell Migration Inhibition; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006801:Humans; D008870:Microtubules / Q000187:drug effects; D009504:Neutrophils / Q000276:immunology; D011188:Potassium / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology / Q000506:poisoning
D000880:Anthraquinones; D014747:Vinblastine; D011188:Potassium; C020491:rhein