Effects of eicosatetraynoic acid on membrane lipids of trout liver and intestine.
After two months feeding either an (n-3) or an (n-6) fatty acid-rich diet, two groups of trout were switched to the (n-6) or the (n-3) fatty acid-rich diet, respectively. Half of each group was treated with 0.03% 5,8,11,14-eicosatetraynoic acid (ETYA) in the diet. Liver and intestinal brush border membrane lipids were analyzed. No effect was observed on their cholesterol content. ETYA induced an accumulation of 18:2(n-6), and it did not affect the 20:4(n-6) content but decreased the 22:5(n-6) content. ETYA induced an increase of 18:3(n-3) content in the brush border membrane and a decrease of the 22:6(n-3) content in the liver. Those results suggest that ETYA blocks mainly the delta 6-desaturase, which should have two different sites in the liver and one in the intestine.
Pelletier
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Centre de Neurochimie, Centre National de la Recherche Scientifique, Strasbourg, France.
Leray
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C
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology*; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D014337:Trout
D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D008563:Membrane Lipids; D010743:Phospholipids; D015117:5,8,11,14-Eicosatetraynoic Acid