The influence of amphotericin B on differentiation induced by dimethylsulfoxide and actinomycin D in HL60 and Friend cell lines.
We use murine erythroid Friend cells and human promyelocytic HL60 cells to investigate the influence of a transmembrane signal in triggering myeloid or erythroid cell differentiation. Combined treatments were given with dimethylsulfoxide, actinomycin D and amphotericin B, a substance which resembles a deviant membrane lipid and which seems to influence exclusively membrane activity. Our results suggest that a membrane modification alone is sufficient for in vitro HL60 cell differentiation, whereas both a transmembrane and a nuclear signal are necessary for Friend cell differentiation.
Ciomei
M
M
Pastori
W
W
Giuliani
F C
FC
D000666:Amphotericin B / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004357:Drug Synergism; D005622:Friend murine leukemia virus; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*
D003609:Dactinomycin; D000666:Amphotericin B; D004121:Dimethyl Sulfoxide