Sequential expression of proto-oncogenes during a mouse erythroleukemia cell differentiation.
To investigate the possible involvement of proto-oncogenes in the regulation of cell differentiation and proliferation, their transcriptional levels were measured by Northern blot analysis during the process of dimethyl sulfoxide-induced differentiation of mouse erythroleukemia cells. The differentiation into erythrocytes was accompanied by sequential transient expression of the proto-oncogenes in the order of c-fos, c-myb, c-myc and c-k-ras, during the first 48 hr of differentiation induction. Following the transient expression of these proto-oncogenes, adult type sss-globin and embryonic type yl-globin gene transcripts appeared and accumulated in terminally differentiated cells. The expression of ten other proto-oncogenes examined here were not detected, nor dramatically changed during differentiation process.
Todokoro
K
K
Ikawa
Y
Y
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000473:pathology*; D051379:Mice; D011519:Proto-Oncogenes; D014158:Transcription, Genetic
D005914:Globins; D004121:Dimethyl Sulfoxide