Effects of busulfan on the DNA of HL-60 cells as a drug sensitivity assay.
We evaluated the formation of DNA-DNA cross links by busulfan as an assay for determining the sensitivity of cells to the drug. HL-60 cells were incubated with busulfan and the effects of the drug on cell growth were compared with the effects of the drug on the alkaline elution pattern of the DNA. While incubation with 100 micrograms/ml for 1 h inhibited cell growth by 50%, drug concentrations of up to 500 micrograms/ml had no effect on the elution pattern of the DNA.
Epstein
J
J
Kirdani
R Y
RY
D002066:Busulfan / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004273:DNA, Neoplasm / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy*; D014410:Tumor Stem Cell Assay
D004273:DNA, Neoplasm; D002066:Busulfan