title: Effect of monoclonal antibodies against lipoteichoic acid from the oral bacterium Streptococcus mutans on its adhesion and plaque-accumulation in vitro.
abstract: Five monoclonal antibodies directed against Streptococcus mutans strain JBP lipoteichoic acid (LTA) were characterized. They were all similarly reactive with the immunizing LTA-containing extract, with intact Strep. mutans JBP cells and with LTA purified from Lactobacillus casei. Immobilized anti-LTA antibodies removes LTA from LTA-containing extracts. The binding of antibodies to LTA was inhibited by the aqueous extract but not by the organic extract of de-acylated LTA, indicating reactivity with the polyglycerol-phosphate portion of the molecule. Antibodies were reactive with all serotypes of Strep. mutans, as well as with strains of Streptococcus salivarius, Streptococcus sanguis and L. casei, but not with LTA-negative species Streptococcus mitis or Actinomyces viscosus. Anti-LTA antibodies at doses of 0.3 or 3.0 micrograms/ml, had no effect on the adherence of Strep. mutans JBP to experimental salivary pellicles formed on hydroxyapatite, but enhanced adherence 150-300 per cent at 30 micrograms/ml. There was no effect of anti-LTA antibodies in a chemostat model which measured sucrose-dependent plaque accumulation by Strep. mutans. The results argue against a major role for LTA in Strep. mutans adherence or plaque accumulation in vitro.
authors.forename: P
authors.initials: P
authors.lastname: Stashenko
authors.forename: W J
authors.initials: WJ
authors.lastname: Peros
authors.forename: R J
authors.initials: RJ
authors.lastname: Gibbons
authors.forename: S M
authors.initials: SM
authors.lastname: Dearborn
mesh_terms: D000268:Adhesiveness; D000907:Antibodies, Bacterial / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000502:physiology*; D003773:Dental Plaque / Q000382:microbiology; D008070:Lipopolysaccharides; D010712:Phosphatidic Acids / Q000276:immunology* / Q000494:pharmacology; D013295:Streptococcus mutans / Q000276:immunology* / Q000502:physiology; D013682:Teichoic Acids / Q000276:immunology* / Q000494:pharmacology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D010712:Phosphatidic Acids; D013682:Teichoic Acids; C009900:lipoteichoic acid