Hypoxanthine:guanine phosphoribosyltransferase activity in xenografts of human osteosarcoma.
It has recently been reported that human osteosarcomas may lack the purine salvage pathway enzyme, hypoxanthine:guanine phosphoribosyltransferase (EC 2.4.2.8). We have established a quantitative assay for measurement of this enzyme in human osteosarcoma xenografts with analysis of products by thin-layer chromatography. Nucleotidase or phosphatase activity was readily detected and could be abolished by preheating cytosol at 60 degrees C for 10 min and performing the assay at pH 10. Alternatively, the use of 25 mM NaF at pH 7.4 also inhibited this activity. The pH optimum for this enzyme in red blood cell sonicates and tumor cytosols was pH 10. All six human osteosarcoma xenografts contained hypoxanthine:guanine phosphoribosyltransferase activity ranging from 0.97 to 4.06 nmol/min/mg of protein at pH 7.4. Control human red blood cell sonicates demonstrated activity of 0.83 nmol/min/mg of protein. These data demonstrate that human osteosarcoma xenografts contain substantial activities of this purine salvage pathway enzyme.
Meyer
W H
WH
Houghton
J A
JA
Lutz
P J
PJ
Houghton
P J
PJ
D000818:Animals; D004912:Erythrocytes / Q000201:enzymology; D005615:Freezing; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis*; D007291:Inosine Monophosphate / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000201:enzymology*; D014183:Transplantation, Heterologous
D007291:Inosine Monophosphate; D007041:Hypoxanthine Phosphoribosyltransferase