The NADPH-producing pathways (pentose phosphate and malic enzyme) are regulated by the NADPH consumption in rat mammary gland.
We have studied the changes in the activity of the pentose phosphate cycle and the malic enzyme produced by the activation or inhibition of different NADPH-consuming pathways. Kynurenate, an acetyl-CoA-carboxylase inhibitor produced a decrease in the flux through the NADPH-producing pathways pentose phosphate cycle and malic enzyme. Acini (isolated from mammary gland) incubated in the presence of ter-butyl-hydroperoxide, a compound which is metabolized via a NADPH-consuming pathway, showed a substantial increase in the pentose phosphate cycle and the malic enzyme pathways.
Revilla
E
E
Departamento de Bioquímica, Facultad de Farmacia, Universidad de Sevilla, Spain.
Fabregat
I
I
Santa María
C
C
Machado
A
A
D000818:Animals; D004789:Enzyme Activation; D005260:Female; D066298:In Vitro Techniques; D007736:Kynurenic Acid / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000201:enzymology / Q000378:metabolism*; D009249:NADP / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D020122:tert-Butylhydroperoxide
D010545:Peroxides; D009249:NADP; D020122:tert-Butylhydroperoxide; D008291:Malate Dehydrogenase; D007736:Kynurenic Acid