Distribution of interdivisional times in proliferating and differentiating Friend murine erythroleukaemia cells.
The interdivisional times of Friend murine erythroleukaemia cells which are growing continuously, or during terminal erythroid differentiation after exposure to dimethyl sulphoxide (DMSO), were determined by time lapse video photography. The median interdivisional times were found to increase from 11.75 hr before exposure to DMSO, to 24.0 hr at 72 hr after exposure. This increase in median interdivisional time was accompanied by an increase in heterogeneity of interdivisional times (%CV = 8.5----40.8), by an increase in the similarity of sister interdivisional times (ryy = 0.622----0.925), and by a decrease in the fraction of cells observed to divide (F = 1.0----0.807). Cells exposed to DMSO for 72 hr can be induced to divide at least once with nearly normal interdivisional times, if they are resuspended at a tenfold higher cell concentration. Computer simulations of cell cycle regulation, based on the opposing reactions model of Murphy, generate interdivisional time distributions which resemble the experimental data better than the single transition probability model of Smith and Martin.
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D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D013997:Time Factors; D014741:Video Recording
D004121:Dimethyl Sulfoxide