Observation on a 65-kilodalton protein isolated from kanagawa positive strains of Vibrio parahaemolyticus.
Crude hemolysin from four KP+ strains of Vibrio parahaemolyticus belonging to serotype 02:K3 exhibited a major protein band (molecular weight, 65 kilodaltons (kDa] in addition to a previously known thermostable direct hemolysin band (molecular weight, 21 kDa) in SDS - polyacrylamide slab gel electrophoresis. These strains showed maximum virulence leading to 100% mouse lethality within 2-6 h. It is hypothesized that this 65-kDa protein may play a vital role in the pathogenesis of the disease caused by V. parahaemolyticus.
Sarkar
B L
BL
National Institute of Cholera and Enteric Diseases, Calcutta, India.
Kumar
R
R
De
S P
SP
Pal
S C
SC
D001426:Bacterial Proteins / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D012703:Serotyping; D014733:Vibrio / Q000302:isolation & purification / Q000472:pathogenicity*; D014774:Virulence
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins