title: Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs.
abstract: The leaf disc transformation system provides a simple means to score expression of various T-DNA markers within days of infection by Agrobacterium tumefaciens as well as long-term selection for growth of transformed callus and shoots. In this report, we describe the application of this system to evaluation of marker transfer and integration in a comprehensive set of defined avirulent mutants of A. tumefaciens. We conclude that virC is not essential when the T-DNA is present on a binary vector. The most likely explanation for this is that the virC gene product is involved in generation of a T-DNA intermediate.
authors.forename: R B
authors.initials: RB
authors.lastname: Horsch
authors.forename: H J
authors.initials: HJ
authors.lastname: Klee
authors.forename: S
authors.initials: S
authors.lastname: Stachel
authors.forename: S C
authors.initials: SC
authors.lastname: Winans
authors.forename: E W
authors.initials: EW
authors.lastname: Nester
authors.forename: S G
authors.initials: SG
authors.lastname: Rogers
authors.forename: R T
authors.initials: RT
authors.lastname: Fraley
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives / Q000235:genetics; D002478:Cells, Cultured; D009154:Mutation; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics / Q000472:pathogenicity*; D014170:Transformation, Genetic
chemical_list: C008666:nopaline; C005394:octopine; D001120:Arginine