[Effect of brovincamine fumarate on cerebral ischemia acutely induced by BLCL in SHRSR].
The present study was designed to clarify the effect of brovincamine fumarate (BV 26-723: BV) on the degree of cerebral ischemia acutely induced by bilateral common carotid artery ligation (BLCL) in stroke-resistant spontaneously hypertensive rats (SHRSR). BV was administered to SHRSR by intraperitoneal infusion (I.P.) of 30 mg/kg (BV 30 mg/kg group), 60 mg/kg (BV 60 mg/kg group) and 0.9% saline was similarly injected to SHRSR (control group) before and immediately after BLCL. Cerebral blood flow (rCBF) in the thalamus was measured by hydrogen clearance technique before and until 3 hr of BLCL periodically. The brain metabolites (ATP, lactate, pyruvate) were determined by the enzymatic method and the brain water content was measured by freeze-dry method 3 hr after BLCL. The histopathological changes in brain vessels were observed by scanning electron microscopy (SEM) 3 hr after BLCL. The rCBF of three groups were identical before BLCL. However, the rCBF of BV 30 mg/kg group was statistically higher than in control group until 2 hr after BLCL, and that of BV 60 mg/kg group was significantly higher even 3 hr after BLCL. In measurements of the brain metabolites after BLCL, ATP and pyruvate levels in both the BV 30 mg/kg and 60 mg/kg groups were statistically higher than the control group. And brain lactate concentrations in both the BV 30 mg/kg and 60 mg/kg groups were significantly lower than the control group. The brain water content of BV 30 mg/kg and 60 mg/kg groups were significantly lower then the control group after BLCL.(ABSTRACT TRUNCATED AT 250 WORDS)
Suzuki
S
S
Second Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
Memezawa
H
H
Katayama
Y
Y
Shimizu
J
J
Sugimoto
S
S
Nagazumi
A
A
Terashi
A
A
D000208:Acute Disease; D000818:Animals; D001921:Brain / Q000378:metabolism; D001929:Brain Edema / Q000378:metabolism; D002545:Brain Ischemia / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D002339:Carotid Arteries / Q000601:surgery; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D008026:Ligation; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D014748:Vinca Alkaloids / Q000627:therapeutic use*; D014749:Vincamine / Q000031:analogs & derivatives / Q000627:therapeutic use*
D014748:Vinca Alkaloids; D014749:Vincamine; C033581:11-bromovincamine