title: Role of sialic acid in synaptosomal transport of amino acid transmitters.
abstract: Active, high-affinity, sodium-dependent uptake of gamma-aminobutyric acid and of the acidic amino acid D-aspartate was inhibited by pretreatment of synaptosomes with neuraminidase from Vibrio cholerae. Inhibition was of a noncompetitive type and was related to the amount of sialic acid released. The maximum accumulation ratios of both amino acids (intracellular [amino acid]/extracellular [amino acid]) remained largely unaltered. Treatment with neuraminidase affected neither the synaptosomal energy levels nor the concentration of internal potassium. It is suggested that the gamma-aminobutyric acid and acidic amino acid transporters are glycosylated and that sialic acid is involved in the operation of the carrier proteins directly and not through modification of driving forces responsible for amino acid uptake.
authors.forename: M M
authors.initials: MM
authors.lastname: Zaleska
authors.forename: M
authors.initials: M
authors.lastname: Erecińska
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D001692:Biological Transport; D001921:Brain / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D011188:Potassium / Q000032:analysis; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism / Q000502:physiology*; D012964:Sodium / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D012794:Sialic Acids; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D012964:Sodium; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D011188:Potassium