title: [Structure of illexine I2 and its complexes with DNA].
abstract: Conformational peculiarities of illexine I2 both in the solution and in the complexes with DNA were studied by circular dichroism, UV-spectroscopy and spectrophotometric melting. IIlexine I2 is shown to have an extended left-handed helical conformation of poly-L-proline II type, that are stable in a wide range of experimental conditions. Upon interaction of illexine I2 with DNA, the parameters of conformation are somewhat distorted but the main peculiarities remain. The DNA double helix changes from B- to the divection of C-form at its interaction with illexine I2. The interaction of illexine I2 with DNA at low ionic strength is non-cooperative and is characterized by some specificity to A--T sequences of DNA. Illexine I2 strongly affects the DNA stability by increasing the melting temperature of DNA.
authors.forename: E I
authors.initials: EI
authors.lastname: Ramm
authors.forename: G S
authors.initials: GS
authors.lastname: Ivanov
authors.forename: V I
authors.initials: VI
authors.lastname: Vorob'ev
authors.forename: S N
authors.initials: SN
authors.lastname: Kadura
authors.forename: S N
authors.initials: SN
authors.lastname: Khrapunov
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002942:Circular Dichroism; D004247:DNA / Q000032:analysis*; D049832:Decapodiformes; D004587:Electrophoresis, Agar Gel; D008297:Male; D009687:Nuclear Proteins; D009691:Nucleic Acid Denaturation; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013094:Spermatozoa / Q000032:analysis*
chemical_list: D000596:Amino Acids; D009687:Nuclear Proteins; D011506:Proteins; C055546:illexine I(2); D004247:DNA
vernacular_title: Issledovanie struktury illeksina I2 i ego kompleksov s DNK.