[Anti-alpha-gliadin antibodies. Sensitivity, specificity and correlation with blood xylose test in the 3 diagnostic stages of celiac disease in children].
The specificity and sensibility of IgA and IgG alfagliadin antibody test (AaGA) for screening, diagnosis and follow-up of childhood coeliac disease has been evaluated. We have compared AaGA test to D-xylose blood test and at last we have examined the false positive and negative results given by the test. Two groups of subjects were considered: 1) 90 children with untreated coeliac disease (21 newly diagnosed (I stage), 50 in gluten withdrawal (II stage), 19 in challenge (III stage); 2) 255 disease controls including: 157 healthy controls; 31 children with gastroenterological disorders other than coeliac disease; 31 children with food allergy and atopic dermatitis; 36 children with "constitutional" short stature (without GH deficiency and with normal intestinal mucosa). The sensibility of AaGA test in the first stage of coeliac disease has been of 95.2% for the IgG class antibody and 90.4% for the IgA class; on the other hand the showed a specificity of 83.6% for IgG class antibody and 96.9% for IgA class. In only two newly diagnosed coeliac children we have found false negative results: in the first case the patient was IgA-deficient, in the second the age was above 3 years. AaGA IgA resulted positive only in the 12.9% of the group of gastroenterological and atopic controls; particularly most cases were affected by multiple food allergies and two patients by chronic autoimmune disease of small intestine.(ABSTRACT TRUNCATED AT 250 WORDS)
Ventura
A
A
Clinica Pediatrica, Istituto per l'Infanzia di Trieste, Italia.
Florean
P
P
Trevisan
M
M
Not
T
T
Perticarari
S
S
Quadrifoglio
F
F
Torre
G
G
Cauci
S
S
D000293:Adolescent; D002446:Celiac Disease / Q000097:blood / Q000175:diagnosis* / Q000178:diet therapy / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000276:immunology; D005188:False Negative Reactions; D005189:False Positive Reactions; D005903:Gliadin / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007223:Infant; D010940:Plant Proteins; D014994:Xylose / Q000097:blood*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D010940:Plant Proteins; D005903:Gliadin; D014994:Xylose
Anticorpi antialfagliadina. Sensibilità, specificità e correlazione con la xilosemia da carico nelle tre fasi diagnostiche della malattia celiaca nel bambino.