Characteristics of the yellow pigment from a strain of Yersinia enterocolitica biovar 1.
Yersinia enterocolitica 195A14J, a milk-isolated strain of biovar 1, produces a non-diffusible yellow pigment and forms star-shaped colonies when grown on egg-yolk agar at 28 degrees C. Solubility properties and in situ Raman spectrum of the pigment support evidence that it is not a carotenoid, although it contains a 9 (+/- 1) double-bond polyenic chain. Pigmentless variants, also star-shaped, appeared with a frequency of ca.10(-3) when bacteria were grown at 38 degrees C. Agarose gel electrophoresis of DNA extracted from the pigmented strain revealed the presence of a 42-kb plasmid which was lost in pigmentless variants.
Vidon
D J
DJ
Laboratoire de Bactériologie, Université Louis Pasteur, Faculté de Pharmacie, Strasbourg, France.
Delmas
C
C
Merlin
J C
JC
D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D010957:Plasmids; D011090:Polyenes / Q000032:analysis* / Q000302:isolation & purification; D012995:Solubility; D013053:Spectrophotometry; D013059:Spectrum Analysis, Raman; D015008:Yersinia enterocolitica / Q000032:analysis* / Q000166:cytology
D004269:DNA, Bacterial; D011090:Polyenes; C056686:yellow pigment (Yersinia enterocolitica)