Analysis and identification of human parotid salivary proteins by micro two-dimensional electrophoresis and Western-blot techniques.
Using this method, without denaturing agents, over 60 protein components in 2 microliter (about 4 micrograms of protein) of unconcentrated parotid saliva could be detected. After two-dimensional separation, the spots of albumin, secretory IgA, IgG, acid phosphatase, amylase and plasma-originating protein were identified by antibodies with the Western-blot technique.
Mogi
M
M
Hiraoka
B Y
BY
Harada
M
M
Kage
T
T
Chino
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008875:Middle Aged; D008970:Molecular Weight; D010306:Parotid Gland / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D012471:Salivary Proteins and Peptides