Marked granulocytosis in C57BL/6 mice bearing a transplanted BMT-11 fibrosarcoma.
When a 3-methylcholanthrene (CAS: 56-49-5)-induced fibrosarcoma, BMT-11, and its eight clones were transplanted sc into syngeneic C57BL/6 mice, leukemoid reaction characterized by a progressive increase in peripheral white blood cells (WBCs) and by splenomegaly was observed as the tumors grew. The WBC count reached about forty-fold of the normal level, and more than 90% of WBCs were found to be polymorphonuclear leukocytes. The increase in WBCs was correlated with tumor size, and its count decreased to normal level within 7 days after surgical excision of subcutaneous tumors. Moreover, a high level of colony-stimulating activity was detected in the supernatant of BMT-11 culture. These results suggest that the colony-stimulating factor produced by BMT-11 cells caused granulocytosis in mice. This is the first report of the marked degree of granulocytosis induced by a transplanted tumor in C57BL/6 strain mice.
Ishikawa
M
M
Hosokawa
M
M
Oh-hara
N
N
Niho
Y
Y
Kobayashi
H
H
D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D003114:Colony-Forming Units Assay; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000473:pathology*; D006098:Granulocytes / Q000473:pathology*; D007958:Leukocyte Count; D008264:Macrophages / Q000473:pathology; D008748:Methylcholanthrene; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D013156:Splenectomy
D008748:Methylcholanthrene