Differing effects of chronic ethanol consumption by mice on liver microsomal metabolism of xenobiotics: 1-nitropyrene, nicotine, aniline, and N-nitrosopyrrolidine.
The effect of ethanol consumption by male CF-1 mice on liver microsomal enzyme activities has been investigated. The total microsomal cytochrome P-450 content was increased by 38%, while cytochrome b5 was decreased by 31%, which are characteristic alterations in liver microsomes following ethanol consumption. Other alterations included a decreased NADPH cytochrome c reductase activity and increased NADPH-supported rates of N-nitrosopyrrolidine and aniline hydroxylation. While ethanol consumption did not alter the total metabolism of nicotine, the rates of N- and C-hydroxylation were differently affected. The 5'-hydroxylation of nicotine was increased by 83%, while the N'-oxidation was decreased by 31%. Changes in the microsomal metabolism of the environmental carcinogen 1-nitropyrene included a slight reduction in the overall metabolism, which can be accounted for by a reduction in the formation of one phenolic metabolite, 1-nitropyren-3-ol.
Howard
P C
PC
Department of Environmental Health Sciences, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106.
DeMarco
G J
GJ
Consolo
M C
MC
McCoy
G D
GD
D000437:Alcoholism / Q000378:metabolism*; D000814:Aniline Compounds / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003573:Cytochrome b Group / Q000378:metabolism; D015786:Cytochromes b5; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D009242:N-Nitrosopyrrolidine / Q000378:metabolism*; D009538:Nicotine / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism*; D010084:Oxidation-Reduction; D011721:Pyrenes / Q000378:metabolism*
D000814:Aniline Compounds; D003573:Cytochrome b Group; D009602:Nitrosamines; D011721:Pyrenes; D009538:Nicotine; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; C023650:aniline; D009242:N-Nitrosopyrrolidine; C032668:1-nitropyrene