Inhibition of microsomal NAD(P)H oxidation by Triton X-100.
The non-ionic detergent Triton X-100 is shown to inhibit the spontaneous oxidation of NAD(P)H associated with rat liver microsomes. Advantage of this observation is taken to measure different microsomal NAD(P)H-dependent oxidoreductase activities such as 3-alpha-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase and various xenobiotic oxidoreductases. This inhibition provides an easy method for the screening of the under-investigated microsomal oxidoreductive metabolism of xenobiotics.
Boutin
J A
JA
D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors; D000818:Animals; D001711:Biotransformation / Q000187:drug effects*; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors*; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D013729:Terpenes / Q000378:metabolism
D013729:Terpenes; D011092:Polyethylene Glycols; C048339:camphoroquinone; D017830:Octoxynol; D006899:Mixed Function Oxygenases; D015096:3-Hydroxysteroid Dehydrogenases; D009247:NADH, NADPH Oxidoreductases