The nucleotide sequence of complementary DNA and the deduced amino acid sequence of peroxisomal catalase of the yeast Candida tropicalis pK233.
We report the isolation and nucleotide (nt) sequence determination of cDNA encoding peroxisomal catalase (Cat) from the yeast Candida tropicalis pK233. The catalase cDNA (Cat) has a single open reading frame (ORF) of 1455 nt, encoding a protein of 484 amino acids (aa), not including the initiator methionine. The Mr of the protein is 54767. Codon use in the gene is not random, with 90.9% of the aa specified by 25 principal codons. The principal codons used in the expression of Cat in C. tropicalis are similar to those used in the expression of the fatty acyl-CoA oxidase gene of C. tropicalis and of highly expressed genes in Saccharomyces cerevisiae. Cat shows 48.0%, 49.7%, and 48.3% aa identity with human, bovine, and rat catalases, respectively, and 44.3% aa identity with catalase T of S. cerevisiae. The 3 aa of bovine liver catalase previously postulated to participate in catalysis and 79.5% of those aa in the immediate environment of hemin, the prosthetic group of catalase, are conserved in Cat of C. tropicalis.
Murray
W W
WW
Department of Biochemistry, McMaster University, Hamilton, Ont., Canada.
Rachubinski
R A
RA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000201:enzymology / Q000235:genetics*; D002374:Catalase / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D008830:Microbodies / Q000201:enzymology; D008969:Molecular Sequence Data
D003062:Codon; D004271:DNA, Fungal; D004247:DNA; D002374:Catalase