title: A rapid, simple method for isolation of viable microfilariae.
abstract: We describe a rapid, efficient method for purifying Loa loa microfilariae from blood that does not affect their longterm motility. It is based on the separation of microfilariae on a performed discontinuous Percoll gradient, followed by filtration on cellulose ester membranes. The parasite population, after isolation, is close to 100% pure, 100% viable and remains motile for at least 20 days. Recovery from original blood samples is typically around 90%. The method, because of its simplicity and minimal material requirements, could be used with up to 210 ml of blood. It also allows the isolation of Mansonella perstans microfilariae.
authors.forename: M
authors.initials: M
authors.lastname: Van Hoegaerden
authors.forename: B
authors.initials: B
authors.lastname: Ivanoff
mesh_terms: D000818:Animals; D002500:Centrifugation, Isopycnic; D005368:Filariasis / Q000469:parasitology; D006801:Humans; D008117:Loa / Q000302:isolation & purification*; D008118:Loiasis / Q000469:parasitology; D008366:Mansonella / Q000302:isolation & purification*; D008368:Mansonelliasis / Q000469:parasitology