Cross-reactivity between human and feline Ia antigens, using a monoclonal antibody HLA-D.m1.
A murine monoclonal antibody HLA-D.m1 produced against human Ia antigens which react with 10% to 30% of human peripheral-blood mononuclear cells was found to react with greater than 90% of feline mononuclear cells. The antigen was not expressed on granulocytes. By direct immunofluorescence, 7% to 41% of feline lymphocytes expressed surface immunoglobulins. In mixed-lymphocyte reaction, the addition of antibody HLA-D.m1 to cultures abolished proliferation. These studies indicate that most feline peripheral-blood mononuclear cells express Ia-like antigens.
Kuramochi
T
T
Takeishi
M
M
Ishida
T
T
Kato
K
K
Ishida
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002415:Cats / Q000276:immunology*; D003429:Cross Reactions; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II