Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from Haemophilus pleuropneumoniae serotype 2.
The phenol-phase soluble cellular lipopolysaccharide that was isolated by the phenol-water extraction from Haemophilus pleuropneumoniae serotype 2 was shown to be of the S type by sodium dodecyl sulfate--polyacrylamide gel electrophoresis, hydrolysis, methylation, specific degradations, and both one- and two-dimensional 1H and 13C nuclear magnetic resonance studies. It could be cleaved to yield a lipid A and an O-chain polysaccharide. This O-polysaccharide was identified as a high molecular weight unbranched linear polymer of a pentasaccharide repeating unit having the structure: (Formula: see text).
Altman
E
E
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ont.
Brisson
J R
JR
Bundle
D R
DR
Perry
M B
MB
D006190:Haemophilus / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D019800:Phenol; D010636:Phenols
D008070:Lipopolysaccharides; D010636:Phenols; D019800:Phenol