Early replication and expression of oocyte-type 5S RNA genes in a Xenopus somatic cell line carrying a translocation.
In Xenopus somatic cells, the somatic-type 5S RNA genes replicate early in S phase, bind the transcription factor TFIIIA, and are expressed; in contrast, the late replicating oocyte-type genes do not bind TFIIIA and are transcriptionally inactive. These facts support a model in which the order of replication of the somatic-type versus the oocyte-type 5S genes causes their differential expression in somatic cells due to sequestration of TFIIIA by the early-replicating somatic genes. Here we provide further evidence for the model by showing that in one Xenopus cell line in which some oocyte-type 5S genes are translocated, some oocyte-type 5S genes replicate early and are expressed.
Guinta
D R
DR
Tso
J Y
JY
Narayanswami
S
S
Hamkalo
B A
BA
Korn
L J
LJ
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D005260:Female; D005786:Gene Expression Regulation; D007399:Interphase; D008957:Models, Genetic; D009865:Oocytes; D012335:RNA, Ribosomal / Q000096:biosynthesis* / Q000235:genetics; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014178:Translocation, Genetic; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism
D012335:RNA, Ribosomal; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors