Characterization of radioactivity in plasma after intraduodenal administration of 131I-labelled human cationic trypsin.
Active human pancreatic cationic trypsin labelled with 131I was administered into the duodenum in nine healthy individuals pretreated with "cold", i.e. non-radioactive iodine. Radioactivity in plasma was measured during 72 hours and was present in plasma after 15 minutes with a maximum 1 hour after administration. The recovered radioactivity in plasma was characterized using dialysis and gel-filtration and the radioactive iodine was found to be in the form of free 131I. Our results are in agreement with recent experimental studies indicating the presence of de-iodinases in the intestine. Furthermore, it was found that pretreatment with "cold" iodine prevented isotope binding to circulating plasma proteins.
Bohe
M
M
Borgström
A
A
Genell
S
S
Ohlsson
K
K
D000328:Adult; D002850:Chromatography, Gel; D003956:Dialysis; D006801:Humans; D007408:Intestinal Absorption; D007455:Iodine; D007457:Iodine Radioisotopes / Q000097:blood / Q000378:metabolism*; D008875:Middle Aged; D010179:Pancreas / Q000378:metabolism; D014357:Trypsin / Q000097:blood / Q000378:metabolism*
D007457:Iodine Radioisotopes; D007455:Iodine; D014357:Trypsin