The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants.
The chain specificities of 18 Ak and 26 Ab-reactive anti-Ia monoclonal antibodies have been determined. L cells were transfected with haplotype-matched (A alpha k:A beta k, A alpha b:A beta k) or haplotype-mismatched (A alpha k:A beta b, A alpha b:A beta k) cDNA pairs, lines expressing high levels of surface A complex were selected, and antibody reactivity with a panel of reagents was assessed by cytofluorimetric analysis. Most of the antibodies recognized a determinant specified by one chain, either alpha or (more commonly) beta. A few examples of more complex determinants were also observed. A knowledge of the chain specificities of anti-Ia monoclonal antibodies should prove useful for a variety of studies aimed at dissecting Ia structure-function relationships.
Landias
D
D
Beck
B N
BN
Buerstedde
J M
JM
Degraw
S
S
Klein
D
D
Koch
N
N
Murphy
D
D
Pierres
M
M
Tada
T
T
Yamamoto
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007739:L Cells; D046911:Macromolecular Substances; D051379:Mice; D014162:Transfection
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances