Chromosome changes associated with spontaneous phenotypic variation of transplantable melanoma.
The chromosome constitutions of black-melanotic (Ma), brown-melanotic (MI), and amelanotic (Ab) melanomas of the Syrian hamster were compared. The MI and Ab melanomas arose through a spontaneous phenotypic alteration of the Ma tumor. All three variants differ in their growth rates, with MI showing the slowest, Ab the fastest, and Ma intermediate growth rate. Cytogenetic examination revealed that each tumor line shows a distinct karyotype. The Ma tumor is near-diploid, whereas, Ab and MI tumors are hypertriploid and near-tetraploid, respectively. Each tumor line shows a unique set of marker chromosomes, though some markers are shared by two different tumor lines. No single marker chromosome was common for all three melanoma variants. We conclude that the spontaneous phenotypic variation of transplantable hamster melanomas is associated with profound changes in the chromosome constitution of the neoplastic cells. The general direction of these changes is toward increased ploidy and increased complexity of the structural abnormalities.
Limon
J
J
Gibas
Z
Z
Babinska
M
M
Beil
B
B
Bomirski
A
A
Mierzewski
P
P
Sandberg
A A
AA
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D006224:Cricetinae; D005819:Genetic Markers; D014644:Genetic Variation; D007621:Karyotyping; D008545:Melanoma / Q000235:genetics*; D008647:Mesocricetus; D009368:Neoplasm Transplantation; D010641:Phenotype
D005819:Genetic Markers