title: The NADPH-producing pathways (pentose phosphate and malic enzyme) are regulated by the NADPH consumption in rat mammary gland.
abstract: We have studied the changes in the activity of the pentose phosphate cycle and the malic enzyme produced by the activation or inhibition of different NADPH-consuming pathways. Kynurenate, an acetyl-CoA-carboxylase inhibitor produced a decrease in the flux through the NADPH-producing pathways pentose phosphate cycle and malic enzyme. Acini (isolated from mammary gland) incubated in the presence of ter-butyl-hydroperoxide, a compound which is metabolized via a NADPH-consuming pathway, showed a substantial increase in the pentose phosphate cycle and the malic enzyme pathways.
authors.affiliation: Departamento de Bioquímica, Facultad de Farmacia, Universidad de Sevilla, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Revilla
authors.forename: I
authors.initials: I
authors.lastname: Fabregat
authors.forename: C
authors.initials: C
authors.lastname: Santa María
authors.forename: A
authors.initials: A
authors.lastname: Machado
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D005260:Female; D066298:In Vitro Techniques; D007736:Kynurenic Acid / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000201:enzymology / Q000378:metabolism*; D009249:NADP / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D020122:tert-Butylhydroperoxide
chemical_list: D010545:Peroxides; D009249:NADP; D020122:tert-Butylhydroperoxide; D008291:Malate Dehydrogenase; D007736:Kynurenic Acid