Aggregation of human salivary Ca-proteinates in the presence of simple carbohydrates in vitro.
The effect of 8 polyols and 14 aldoses or ketoses on the spontaneous aggregation of Ca-proteinates was followed spectrophotometrically in supernatants and filtrates of human mixed saliva. Each carbohydrate was added to the saliva samples at 37 degrees C and the precipitated material was analyzed for protein, total carbohydrate and Ca. Based on their effect on aggregation, the carbohydrates could be divided into three groups: 1) those that showed no effect on aggregation: D-xylose, D-ribose and i-erythritol, 2) those that inhibited aggregation strongly: xylitol, D-sorbitol and D-mannitol, and 3) those that inhibited aggregation moderately: glucose, fructose and sucrose. The inhibitory effect of the above polyols on the aggregation of Ca-proteinates varied greatly among the saliva donors, and correlated positively with the turbidity of the saliva and its protein content more than with the Ca-concentration or the pH of the saliva sample. It is suggested that inhibition of aggregation shown the most clearly for xylitol, sorbitol and mannitol manifests itself as a retardation of the final, irreversible aggregation of those glyco-proteins that already exist in a precipitated form and which are responsible for the turbidity of saliva.
Söderling
E
E
Mäkinen
K K
KK
D000328:Adult; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005260:Female; D006801:Humans; D008297:Male; D009005:Monosaccharides / Q000494:pharmacology*; D012463:Saliva / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism* / Q000502:physiology; D013012:Sorbitol / Q000494:pharmacology; D013053:Spectrophotometry; D013402:Sugar Alcohols / Q000494:pharmacology*; D013997:Time Factors; D014993:Xylitol / Q000494:pharmacology
D009005:Monosaccharides; D012471:Salivary Proteins and Peptides; D013402:Sugar Alcohols; D013012:Sorbitol; D002118:Calcium; D014993:Xylitol