Purification and characterization of erythroid differentiation factor (EDF) isolated from human leukemia cell line THP-1.
We isolated a protein, from a cell line of human origin, which exhibits extensive differentiation inducing activity toward Friend leukemia cells. The protein, called Erythroid Differentiation Factor (EDF), was found in a 4 day culture of THP-1 cells performed in the presence of 4 beta-phorbol 12-myristate 13-acetate(PMA). EDF is a homodimer of a molecular weight of 25,000, with an NH2-terminal sequence identical to that of the beta A-chain of porcine Inhibin. It was suggested that a single protein species is responsible for the activities of both EDF and FRP, a FSH releasing protein isolated from porcine ovarian follicular fluid.
Eto
Y
Y
Tsuji
T
T
Takezawa
M
M
Takano
S
S
Yokogawa
Y
Y
Shibai
H
H
D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005622:Friend murine leukemia virus; D006801:Humans; D007265:Inhibins / Q000302:isolation & purification*; D007938:Leukemia / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D007942:Leukemia, Experimental / Q000473:pathology; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D010446:Peptide Fragments; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D046911:Macromolecular Substances; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D028341:Activins; D007265:Inhibins; D013755:Tetradecanoylphorbol Acetate