title: Identification and characterization of a differentiation-specific antigen on normal and malignant murine erythroid cells.
abstract: Rauscher murine erythroleukemia cells grow continuously in vitro and undergo terminal differentiation in response to the physiological inducer erythropoietin. In the course of this developmental process they express many erythroid-specific markers. In order to investigate the expression of cell surface determinants during Rauscher cell differentiation we generated monoclonal antibodies to uninduced cells. Using an anti-Rauscher cell monoclonal antibody, we have identified a cell surface determinant, designated ERY-1, that is present on normal murine erythroid cells. This determinant is apparently absent from the early progenitor BFU-E, but is present on the more mature progenitors CFU-E and CFC-E. It disappears during erythroid maturation and is absent from the mature erythrocyte. This pattern of ERY-1 expression is exhibited with remarkable fidelity during the erythropoietin-induced differentiation of Rauscher cells. Such differentiation-specific expression of the ERY-1 determinant suggests that it may play a functional role in erythropoiesis.
authors.forename: E R
authors.initials: ER
authors.lastname: Bacon
authors.forename: A J
authors.initials: AJ
authors.lastname: Sytkowski
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology*; D004920:Erythropoiesis / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology* / Q000473:pathology; D051379:Mice; D010659:Phenylhydrazines / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D010659:Phenylhydrazines; C030299:phenylhydrazine; D004921:Erythropoietin