title: The role of purines in the maintenance of meiotic arrest in mouse oocytes.
abstract: Meiotic arrest of mammalian oocytes within ovarian follicles is maintained by a specific factor(s) within the follicle. There is strong evidence that cAMP plays an important role in the control of meiosis. Purines have also been implicated in the maintenance of meiotic arrest in vivo. Hypoxanthine and/or adenosine have been identified in pig and mouse follicular fluid and exert a meiosis-arresting action on mouse oocytes in culture. While adenosine apparently need not be metabolized to exert its action on oocyte maturation, the action of hypoxanthine is apparently due to the production of guanyl and/or xanthyl compounds by the oocyte-cumulus cell complex. The inosine monophosphate dehydrogenase inhibitors, mycophenolic acid and bredinin, induced maturation in cumulus cell-enclosed oocytes maintained in meiotic arrest by hypoxanthine. Hypoxanthine and adenosine are not toxic to oocytes, because oocytes undergo normal fertilization and pre- and post-implantation development following exposure to these molecules in vitro. It is not known how gonadotropins stimulate the resumption of meiosis within the follicle, but there are several possibilities: (1) the intrafollicular level of an oocyte maturation inhibitor is decreased; (2) the oocyte is uncoupled from surrounding follicle cells; (3) an inhibitory molecule is secreted or metabolized by the oocyte; and/or (4) a positive stimulus is produced by the follicle that overrides the presence of inhibitory molecules. Preliminary evidence suggests that cumulus cells may produce a positive stimulus that induces the maturation of cultured cumulus cell-enclosed oocytes. Whether germinal vesicle breakdown in vivo results from a positive induction, a loss of inhibitory input, or a combination of these two mechanisms remains to be determined.
authors.affiliation: Jackson Laboratory, Bar Harbor, ME 04609.
authors.forename: S M
authors.initials: SM
authors.lastname: Downs
authors.forename: J J
authors.initials: JJ
authors.lastname: Eppig
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001826:Body Fluids / Q000378:metabolism / Q000502:physiology; D005260:Female; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D051379:Mice / Q000033:anatomy & histology / Q000502:physiology*; D009865:Oocytes / Q000166:cytology*; D009994:Osmolar Concentration; D006080:Ovarian Follicle / Q000378:metabolism; D011687:Purines / Q000378:metabolism / Q000502:physiology*; D013552:Swine
chemical_list: D007042:Hypoxanthines; D011687:Purines; D019271:Hypoxanthine; D000241:Adenosine