Synthetic human parathyroid hormone fragment stimulates prostaglandin E2 synthesis by chick calvariae.
Synthetic human PTH N-terminal 1-34 peptide [hPTH-(1-34)] stimulates prostaglandin (PG) production by chick calvariae in culture. PGE2 was the predominant PG found by both RIA and HPLC. Stimulation of PGE2 synthesis was significant at 50 ng/ml (1.2 X 10(-8) M) hPTH-(1-34) and was dose dependent at concentrations up to 0.6 microgram/ml (1.4 X 10(-7) M). Continuous exposure of calvariae to hPTH-(1-34) showed that PGE2 production increased significantly by 1 h, reached a maximum at 24 h, and then persisted over 96 h. Indomethacin at concentrations above 5 X 10(-7) M inhibited PGE2 synthesis by both control and hPTH-(1-34)-treated bones. PTH-(1-34) stimulated bone cells to convert arachidonic acid to PGE2, but did not activate the bone to release stored arachidonate. In summary, our results show that hPTH-(1-34) stimulates PGE2 synthesis by chick calvariae. This endogenous PGE2 may be involved in bone remodeling.
Yang
C Y
CY
Gonnerman
W A
WA
Taylor
L
L
Nimberg
R B
RB
Polgar
P R
PR
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D001862:Bone Resorption / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D002415:Cats; D015237:Dinoprost; D015232:Dinoprostone; D006728:Hormones / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D009924:Organ Culture Techniques; D010281:Parathyroid Hormone / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis; D019379:Teriparatide; D013929:Thromboxane B2 / Q000096:biosynthesis
D006728:Hormones; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D011458:Prostaglandins E; D011460:Prostaglandins F; D019379:Teriparatide; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin