The complete primary structure of the rat A-raf cDNA coding region: conservation of the putative regulatory regions present in rat c-raf.
A-raf gene was first detected by low-stringent hybridization with v-raf and was reported as a possible oncogene. We have cloned rat A-raf cDNA and determined the complete nucleotide sequence over its long open reading frame. Alignment of rat A-raf- and c-raf-deduced amino acid sequences shows 59.4% homology. Both the ATP binding site and the kinase domain are conserved in A-raf as in c-raf. Furthermore, three regions highly conserved between A-raf and c-raf were identified in the 5'-half region of c-raf which has been shown to be deleted in the activated form of c-raf. These regions were speculated to be the candidates of the regulatory domain controlling the protein kinase activity of raf products. Southern blot analysis shows that genes homologous to A-raf and c-raf are present as independent loci in Drosophila melanogaster, Caenorhabditis elegans, Saccharomyces cerevisiae and Candida boidinii. A-raf was expressed in several tissues and cell line at various amounts.
Ishikawa
F
F
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Takaku
F
F
Nagao
M
M
Sugimura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009856:Oncogene Proteins, Viral / Q000235:genetics; D009857:Oncogenes; D011519:Proto-Oncogenes; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D009856:Oncogene Proteins, Viral; D004247:DNA