In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation.
Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.
Narayanan
R
R
Jastreboff
M M
MM
Chiu
C F
CF
Bertino
J R
JR
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D015500:Chloramphenicol O-Acetyltransferase; D003114:Colony-Forming Units Assay; D004560:Electricity; D005260:Female; D006098:Granulocytes / Q000201:enzymology; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D008808:Mice, Inbred CBA; D010957:Plasmids; D014162:Transfection
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase