Location of the phosphorylation site for casein kinase-2 within the amino acid sequence of ornithine decarboxylase.
The sizes of the radiolabeled fragments obtained by CNBr and DMSO/HBr digestion of 32P-labeled ornithine decarboxylase phosphorylated by rat liver casein kinase TS (type-2) are consistent with the location of the phosphorylation site within the sequence(303-309) Ser-Asp-Asp-Glu-Asp-Glu-Ser. Parallel experiments with synthetic peptides rule out the suitability of Ser-309, as well as of other serines of ornithine decarboxylase having just two or three acidic residues close to their C terminal side. Ser-303 appears, therefore, to be the main if not the only target for casein kinase-2.
Meggio
F
F
Flamigni
F
F
Guarnieri
C
C
Pinna
L A
LA
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D047388:Casein Kinases; D008099:Liver / Q000201:enzymology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000096:biosynthesis; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats
D010455:Peptides; D010768:Phosphoserine; D011494:Protein Kinases; D047388:Casein Kinases; D009955:Ornithine Decarboxylase