Regional localization of 18 human X-linked DNA sequences.
A series of human probes with unique sequences has been isolated from a recombinant phage library constructed with DNA obtained from a human-hamster hybrid cell line. This cell line contained the X chromosome as the only human component. For 18 of these probes, a human X-chromosome origin has been confirmed and they have been regionally assigned by a combination of techniques: dosage studies utilizing DNA from human fibroblasts carrying X-chromosome duplications and deletions; the presence or absence of hybridization to digested DNA from hybrid lines carrying fragments of the X chromosome; and in situ hybridization to metaphase chromosomes. The use of dosage as a means to regionally assign probes significantly improves resolution of the X chromosome.
Riddell
D C
DC
Wang
H S
HS
Beckett
J
J
Chan
A
A
Holden
J J
JJ
Mulligan
L M
LM
Phillips
M A
MA
Simpson
N E
NE
Wrogemann
K
K
Hamerton
J L
JL
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D014960:X Chromosome
D005819:Genetic Markers; D004247:DNA