Augmentation of hydroxyurea cytotoxicity by sintamil in human chronic myeloid leukemia cells.
The in vitro effect of sintamil, as a modulator alone and in combination with hydroxyurea (HU), on cytotoxicity was studied in 16 cases of human chronic myeloid leukemia (CML). We investigated the cytotoxicity of the drugs as a function of the exposure dose (HU, 10(-4) M; sintamil, 10 micrograms/ml) and the exposure time (1 h) to the agent. Cytotoxicity was evaluated as the inhibition of incorporation of [3H-methyl]thymidine in the nucleic acids of CML cells. Cytotoxicity of HU was greatly enhanced (P less than 0.001) by 1 h exposure of the CML cells to sintamil. The present data indicate that sintamil potentiates the cytotoxic activity of HU in CML cells.
Pradhan
S G
SG
Chitnis
M P
MP
Basrur
V S
VS
Satyamoorthy
K
K
Advani
S H
SH
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects; D003989:Dibenzoxazepines / Q000627:therapeutic use*; D004347:Drug Interactions; D006801:Humans; D006918:Hydroxyurea / Q000627:therapeutic use*; D007951:Leukemia, Myeloid / Q000188:drug therapy*
D003989:Dibenzoxazepines; C100140:sintamil; D006918:Hydroxyurea