D-glucose uptake in intestinal brush-border membrane vesicles of rachitic rats.
We have previously reported the metabolic consequences of feeding rats Steenbock and Black's rickets-inducing diet, deficient in vitamin D and with an altered Ca/P ratio. Using isolated brush-border membrane vesicles prepared from the jejunum, ileum and duodenum of control and rachitic rats, we have demonstrated a marked decrease of Na+-dependent D-glucose uptake at jejunum-ileum level of rachitic rats. At duodenum level Na+-dependent D-glucose transport was not influenced by rickets. A lack of any significant difference between the two animal groups was observed studying the facilitated transport of D-glucose, the diffusion of L-glucose and the Na+-dependent uptake of phenylalanine and aspartate.
Treves
C
C
Institute of Biochemistry, University of Florence, Italy.
Favilli
F
F
Iantomasi
T
T
Stio
M
M
Vanni
P
P
Vincenzini
M T
MT
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004386:Duodenum / Q000378:metabolism; D005947:Glucose / Q000493:pharmacokinetics*; D007082:Ileum / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012279:Rickets / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D013997:Time Factors
D012964:Sodium; D005947:Glucose