Picogram measurement of prostaglandin E2 synthesis by gastric mucosa by high-performance liquid chromatography.
Prostaglandin biosynthesis by gastric mucosa was determined by a 1-min incubation, solvent extraction, and reaction with panacyl bromide. The prostaglandin ester was measured by normal-phase high-performance liquid chromatography. The prostaglandin E2 levels of normal gastric mucosa of rats and swine were 90.6 +/- 31.0 and 79.8 +/- 39.8 pg/min/mg tissue, respectively. This method was sensitive to 40 pg and specific for prostaglandin E2.
Stein
T A
TA
Angus
L
L
Borrero
E
E
Auguste
L J
LJ
Wise
L
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone; D005753:Gastric Mucosa / Q000378:metabolism*; D066298:In Vitro Techniques; D011458:Prostaglandins E / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats
D011458:Prostaglandins E; D015232:Dinoprostone