title: Separation and properties of rabbit buccal mucosal wound hyaluronidase.
abstract: This study establishes the existence of a mammalian buccal mucosal wound hyaluronidase (hyaluronate 4-glycohydrolase; EC 3.2.1.35) having properties distinct from those of the endogenous lysosomal hyaluronidase of normal (uninjured) buccal mucosa. A time-dependent change in hyaluronidase activity was measured, with the highest specific activity occurring on post-wound day 4 (7.7 +/- 1.3 units/mg protein), followed by consecutive decreases until activity was no longer discernible by day 21. Mucosal wound hyaluronidase closely resembled a previously described integumentary wound endoglycosidase in terms of a high pH optimum (5.0-6.0), distinct (but non-exclusive) substrate preference for hyaluronic acid, and ability to generate saturated depolymerization products by an endoglycosidic hydrolysis.
authors.forename: C N
authors.initials: CN
authors.lastname: Bertolami
authors.forename: R H
authors.initials: RH
authors.lastname: Day
authors.forename: D G
authors.initials: DG
authors.lastname: Ellis
mesh_terms: D000818:Animals; D002610:Cheek; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D003124:Colorimetry; D006097:Granulation Tissue / Q000201:enzymology*; D006821:Hyaluronoglucosaminidase / Q000302:isolation & purification*; D009061:Mouth Mucosa / Q000201:enzymology* / Q000293:injuries; D011817:Rabbits; D014945:Wound Healing
chemical_list: D006821:Hyaluronoglucosaminidase