Effect of sofalcone on gastric mucosal prostaglandin metabolism in taurocholate induced gastritis in rats.
The effects of sodium taurocholate (TCA) and sofalcone on prostaglandin (PG) metabolism in rat gastric mucosa were examined. TCA administration for 3 months increased the activity of the PG-metabolizing enzyme, 15-hydroxy-PG-dehydrogenase (15-OH-PG-DH) and decreased the PGE2 content in the rat gastric mucosa. The administration of sofalcone for 3 weeks prevented the increase in the 15-OH-PG-DH activity and prevented the decrease in the PGE2 content reduced by TCA administration. The activation and inhibition of 15-OH-PG-DH activity by the administration of TCA and sofalcone were both uncompetitive and non competitive against the substrates PGE1 and NAD, respectively. These results suggest that the decrease in PGE2 content by the activation of 15-OH-PG-DH activity is involved in the development of TCA induced gastritis and the effect of sofalcone which prevented the decrease of mucosal PGE2 by inhibiting 15-OH-PG-DH plays an important role in the healing of gastritis.
Muramatsu
M
M
Tanaka
M
M
Murakami
S
S
Aihara
H
H
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002599:Chalcone / Q000031:analogs & derivatives / Q000494:pharmacology*; D047188:Chalcones; D015232:Dinoprostone; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000139:chemically induced / Q000378:metabolism*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D007700:Kinetics; D008297:Male; D011427:Propiophenones / Q000494:pharmacology*; D011458:Prostaglandins E / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013656:Taurocholic Acid / Q000037:antagonists & inhibitors
D000897:Anti-Ulcer Agents; D047188:Chalcones; D011427:Propiophenones; D011458:Prostaglandins E; C035032:sofalcone; D013656:Taurocholic Acid; D002599:Chalcone; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D015232:Dinoprostone