[Effect of antioxidants on the production of reactive oxygen metabolites by stimulated alveolar macrophages].
Reactive oxygen metabolites (ROM) (O2-, H2O2, 1O2, .OH, OX-) which are produced by stimulated alveolar macrophages (AM), neutrophils and eosinophils, play an important role in the pathogenesis of many acute and chronic lung diseases. With regard to a therapeutic application the influence of the antioxidants ascorbic acid (Vitamin C) and alpha-tocopheryl acetate (Vitamin E acetate) on the production of ROM by AM was investigated. The AM were isolated by bronchoalveolar lavage from patients with different lung disorders. The ROM were determined by means of chemiluminescence-measuring. alpha-Tocopheryl acetate solved in peanut oil causes a little increase of the yeast cell wall-induced chemiluminescence. Pure alpha-Tocopheryl acetate has no effect on the chemiluminescence. In contrast to alpha-Tocopheryl acetate the addition Vitamin C to the stimulated AM results in a strong diminution of the chemiluminescence signal. This result suggests that Vitamin C reduces the generation of ROM by AM. Therefore Vitamin C could be a suitable scavenger of radicals and oxidants in different lung diseases.
Winsel
K
K
Forschungsinstitut für Lungenkrankheiten und Tuberkulose Berlin-Buch/DDR.
Slapke
J
J
Unger
U
U
Grollmuss
H
H
Renner
H
H
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D006801:Humans; D008163:Luminescent Measurements; D008171:Lung Diseases / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects*; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D000975:Antioxidants; D014810:Vitamin E; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; D024505:Tocopherols
Einfluss von Antioxidanzien auf die Erzeugung von reaktiven Sauerstoffmetaboliten durch stimulierte Alveolarmakrophagen.