Specific A . T DNA sequence binding of RP-HPLC purified HMG-I.
HMG-I (alpha-protein) is a high mobility group protein which recognizes and binds specifically to A . T rich double stranded DNA. We have investigated, by electrophoretic shift assays and DNase I footprinting, the ability of reverse-phase high performance liquid chromatography purified HMG-I to bind to specific A . T rich duplex DNA sequences. We show here that when HMG-I is isolated and purified under denaturing conditions it retains its specific A . T DNA binding activity. These results suggest that reverse-phase high performance liquid chromatography to be the method of choice for the preparation of HMG-I.
Elton
T S
TS
Nissen
M S
MS
Reeves
R
R
D000225:Adenine; D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I; D006609:High Mobility Group Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D007942:Leukemia, Experimental / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D013941:Thymine
D006609:High Mobility Group Proteins; D004247:DNA; D003850:Deoxyribonuclease I; D000225:Adenine; D013941:Thymine