Conjugational fertility and location of chloramphenicol biosynthesis genes on the chromosomal linkage map of Streptomyces venezuelae.
In Streptomyces venezuelae fertility, defined as chromosomal gene recombination, was enhanced over 1000-fold when one parent in a biparental conjugational cross lacked the physically-undetected plasmid SVP1, as compared with crosses in which both parents carried SVP1. The existence of SVP1 and at least two other fertility plasmids, SVP2 and SVP3, was detected in S. venezuelae by 'lethal zygosis' elicited by a plasmid-plus mycelium in contact with a plasmid-minus mycelium. Conjugational crosses were used to construct a linkage map of S. venezuelae which was highly consistent with the map of analogous loci in S. coelicolor A3(2). A cluster of genes governing chloramphenicol biosynthesis was located near arg, cys and pdxB genes at a position roughly equivalent to the 1-2 o'clock region of the S. coelicolor A3(2) map.
Doull
J L
JL
Vats
S
S
Chaliciopoulos
M
M
Stuttard
C
C
Wong
K
K
Vining
L C
LC
D002701:Chloramphenicol; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D005798:Genes, Bacterial; D005819:Genetic Markers; D005838:Genotype; D010957:Plasmids; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics*
D005819:Genetic Markers; D002701:Chloramphenicol