Inability of HL-60 cells induced by phorbol myristate acetate to produce macrophage-associated glycosaminoglycans.
Glycosaminoglycans (GAGs) have been implicated in many functions of human blood cells. Normal human monocytes grown on plastic culture dishes for five days display macrophage-like morphology and functional characteristics concurrent with the appearance of a 4,6-disulfated chondroitin. This process has been regarded as in vitro differentiation. HL-60 human promyelocytic leukemia cells produce chondroitin 4-sulfate, which is the major GAG constituent of normal granulocytes and monocytes. These cells can be induced by phorbol myristate acetate (PMA) to develop many macrophage-like characteristics. This study investigates GAG production by HL-60 cells after prolonged exposure to PMA. Disulfated chondroitin molecules were not detected. This biochemical abnormality may be an important factor in the functional deficiencies of these induced cells.
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D002460:Cell Line; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D010704:Phorbols / Q000494:pharmacology*; D013462:Sulfur Radioisotopes; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D006025:Glycosaminoglycans; D010704:Phorbols; D013462:Sulfur Radioisotopes; D013755:Tetradecanoylphorbol Acetate