Dermatan sulfate isomers in human articular cartilage characterized by high-performance liquid chromatography.
The Constituents of dermatan sulfate isomers in human articular cartilage were studied at the disaccharide level by high-performance liquid chromatography. Appreciable amounts of dermatan sulfate components, i.e., dermatan sulfate, chondroitin sulfate types G and B, could be detected after digestion with chondroitinases-B or -ABC. The oversulfated dermatan sulfate isomers were isolated only after digestion with chondroitinase-ABC but not with the AC-lyase. The dermatan sulfate isomers were found to be markedly increased in weight loading parts of articular cartilage. It is postulated that the dermatan sulfate isomers are formed as a result of the weight loading reaction, which may be responsible for the fibrosis of articular cartilage.
Murata
K
K
Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Japan.
Yokoyama
Y
Y
D000328:Adult; D002358:Cartilage, Articular / Q000032:analysis*; D002807:Chondroitin / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid; D003871:Dermatan Sulfate / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis; D004586:Electrophoresis / Q000379:methods; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis; D007536:Isomerism; D008297:Male; D008875:Middle Aged
D004187:Disaccharides; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002807:Chondroitin