In vitro perfusion studies on human umbilical arteries. I. Vasoactive effects of serotonin, PGF2 alpha and PGE2.
A method was developed for the assessment of in vitro perfusion of the umbilical cord arteries. A perfusion pressure of 60-80 mmHg gave flow rates ranging from 30 to 40 ml/min per artery. Serotonin, PGF2 alpha and PGE2 were added to the perfusate and tested for vasoactivity. All substances induced a dose-dependent vasoconstriction. Serotonin proved to be the most potent vasoconstrictor. The minimum dose required to induce visible pressure responses was 10(-9) - 10(-8) M of serotonin and 10(-7) M of PGF2 alpha and PGE2. When adding methysergide to the perfusate, the serotonin response was abolished, while the effects of the prostanoids remained unaltered.
Bjøro
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Stray-Pedersen
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D015237:Dinoprost; D015232:Dinoprostone; D005260:Female; D006801:Humans; D008784:Methysergide / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D010477:Perfusion; D011247:Pregnancy; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology*; D014469:Umbilical Arteries / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D011458:Prostaglandins E; D011460:Prostaglandins F; D014662:Vasoconstrictor Agents; D012701:Serotonin; D015237:Dinoprost; D015232:Dinoprostone; D008784:Methysergide