Effect of platelet-derived growth factor on enriched populations of haemopoietic progenitors from patients with chronic myeloid leukaemia.
The effect of pure platelet-derived growth factor and fresh serum on the in-vitro growth of purified haemopoietic progenitors from the peripheral blood of 12 patients with CML was studied. Purified haemopoietic progenitors were prepared using Percoll separation followed by cell sorting with the monoclonal antibody BI.3C5. Both pure PDGF at a concentration of 20 ng/ml and fresh serum significantly increased the numbers of BFU-E (p less than 0.01) and CFU-GEMM (p less than 0.014), but not the CFU-GM. That the PDGF effect was not mediated to any significant extent via prostaglandins, was shown by the lack of inhibitory effect of indomethacin on the growth of purified progenitor cells in the presence of fresh serum. Increased amounts of pure PDGF were required to give maximal stimulation of purified CML peripheral blood progenitors compared to normal bone marrow progenitors. These results show that CML progenitors are capable of responding to PDGF. Whether the quantitative difference in response is due to a reduced proportion of mesenchymal cells in CML peripheral blood compared to normal marrow, or whether CML progenitors are most likely already stimulated by autocrime PDGF or other growth factors remains to be elucidated.
Katz
F E
FE
Michalevicz
R
R
Lam
G
G
Hoffbrand
A V
AV
Goldman
J M
JM
D000911:Antibodies, Monoclonal; D002455:Cell Division; D002469:Cell Separation; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007951:Leukemia, Myeloid / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
D000911:Antibodies, Monoclonal; D010982:Platelet-Derived Growth Factor; D007213:Indomethacin