Human tumor cells synthesize an endothelial cell growth factor that is structurally related to basic fibroblast growth factor.
A human hepatoma cell line synthesizes, as evidenced by metabolic labeling, an endothelial cell mitogen that is found to be mostly cell associated. The hepatoma-derived growth factor (HDGF) has been purified to homogeneity by a combination of Bio-Rex 70, heparin-Sepharose, and reverse-phase chromatography; it is a cationic polypeptide with a molecular weight of about 18,500-19,000. HDGF is structurally related to basic fibroblast growth factor (FGF). Immunological analysis demonstrates that antiserum prepared against a synthetic peptide corresponding to the amino-terminal sequence of basic FGF cross-reacts with HDGF when analyzed by electrophoretic blotting and by immunoprecipitation. Sequence analysis of tryptic fragments demonstrates that HDGF contains sequences that are homologous to both amino-terminal and carboxyl-terminal sequences of basic FGF.
Klagsbrun
M
M
Sasse
J
J
Sullivan
R
R
Smith
J A
JA
D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000096:biosynthesis / Q000276:immunology; D000818:Animals; D003429:Cross Reactions; D004727:Endothelium / Q000166:cytology; D005346:Fibroblast Growth Factors / Q000276:immunology; D006133:Growth Substances / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008114:Liver Neoplasms, Experimental / Q000503:physiopathology*; D010446:Peptide Fragments / Q000032:analysis; D010902:Pituitary Gland / Q000502:physiology
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; C051610:hepatoma-derived growth factor; D005346:Fibroblast Growth Factors