Susceptibility of the human promyelocytic cell line HL-60 to an endogenous antileukaemic factor in suspension cultures.
HL-60 human leukaemic cell line a suitable homogeneous target population for the selective endogenous inhibitor of myelopoiesis, isolated in our laboratory, was submitted to multiparameter analysis of cell proliferation in suspension cultures. As detected by 3H-TdR incorporation, a single dose of the regulator elicited a 6 to 8 hours arrest of DNA synthesis. The inhibition could be prolonged by repeated applications. As affected by the factor, alteration of population kinetics is characterized, revealed by flow cytofluorometric analysis, in G1 arrest of a fraction of cells, and diminishing those in hyperdiploid and tetraploid stage. 51Cr-release detection of vitality proved, that the endogenous factor, chemically determined as nucleopeptide, affected non-toxically and reversibly HL-60 cell proliferation.
Balázs
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Tapolcai
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Hetényi
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Mann
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Boldog
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D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D004261:DNA Replication; D005434:Flow Cytometry; D006131:Growth Inhibitors / Q000302:isolation & purification*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*
D003470:Culture Media; D006131:Growth Inhibitors; D006918:Hydroxyurea