A radioimmunoassay for deoxycorticosterone sulfate without hydrolysis.
The hapten of deoxycorticosterone sulfate was synthesized and linked to the carrier protein through the C-4 position on the steroid nucleus. The antisera raised against this antigen in guinea pigs exhibited high affinity (Ka = 1.86 x 10(9) M-1) and excellent specificity for deoxycorticosterone sulfate. It was found that deoxycorticosterone sulfate can be determined in the range of 50-5000 pg, and this radioimmunoassay in plasma shows a coefficient of variation (CV) less than 10%.
Tanaka
T
T
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Hachinoe
M
M
Kubodera
A
A
D003900:Desoxycorticosterone / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D006241:Haptens; D006801:Humans; D006868:Hydrolysis; D011863:Radioimmunoassay / Q000379:methods*; D012710:Serum Albumin, Bovine
D006241:Haptens; C032268:deoxycorticosterone sulfate; D012710:Serum Albumin, Bovine; D003900:Desoxycorticosterone