Products of two common alleles at the locus for human placental alkaline phosphatase differ by seven amino acids.
Amino-terminal amino acid sequences (42 residues) were determined for the products of the three common alleles at the human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] gene locus. The sequences differ at position 3, which is proline in types 1 and 2 but is leucine in type 3. cDNA libraries were constructed in phage lambda gt11 and used to isolate clones covering the coding regions of types 1 and 3 cDNAs. Comparison of the deduced amino acid sequences of the types 1 and 3 proteins showed 7 differences out of 513 amino acids, each due to a single base substitution. cDNA sequence comparisons showed three silent substitutions in the coding regions and three base differences in the greater than 1 kilobase pairs of 3' untranslated sequences.
Henthorn
P S
PS
Knoll
B J
BJ
Raducha
M
M
Rothblum
K N
KN
Slaughter
C
C
Weiss
M
M
Lafferty
M A
MA
Fischer
T
T
Harris
H
H
D000469:Alkaline Phosphatase / Q000235:genetics*; D000483:Alleles; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D010920:Placenta / Q000201:enzymology; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D000469:Alkaline Phosphatase