Visualization of proteoglycans and membrane-associated components in rat incisor predentine and dentine using ruthenium hexammine trichloride.
Ruthenium hexammine trichloride (RHT) used as a probe to visualize anion groups in the predentine and dentine of rat incisors, showed a complex distribution pattern including: intercellular proteoglycans (Pg), detected in the predentine as granules 10-15 nm in diameter and as filaments. Non-aggregating Pg was observed in the spaces between collagen fibres as an amorphous group substance. The dentine included smaller RHT-positive granules, observed after thin-section demineralization; pericellular aggregates, 30-50 nm in diameter, which were absent at the onset of the cell coat along the plasma-membrane of the odontoblast process and of the membrane itself. All these RHT-positive components might be Pg and/or sialoglyconjugates and glyco- or phospholipids present on the plasma membrane.
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D000818:Animals; D003804:Dentin / Q000648:ultrastructure*; D003810:Dentinogenesis; D007180:Incisor / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000032:analysis; D008854:Microscopy, Electron; D009804:Odontoblasts / Q000648:ultrastructure; D011509:Proteoglycans / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012428:Ruthenium; D017975:Ruthenium Compounds
D008565:Membrane Proteins; D011509:Proteoglycans; D017975:Ruthenium Compounds; C031584:hexammineruthenium; D012428:Ruthenium