A simple fluorometric method to discriminate adriamycin-resistant subline from adriamycin-sensitive parental P388 murine leukemia cell line.
A fluorometric method to estimate adriamycin accumulation in cells was used to discriminate adriamycin- and vincristine-resistant sublines from sensitive parental P388 murine leukemia cells. It was easily checked that adriamycin accumulation was lower in the adriamycin- and vincristine-resistant cells than in the sensitive ones. With this method we found that tamoxifen as well as diltiazem increased adriamycin accumulation in the resistant cells to a level similar to that in the sensitive ones. The effect of progesterone was much less.
Dekker
K
K
Institute of Applied Biochemistry, Yagi Memorial Park, Gifu, Japan.
Kojima
N
N
Yamamoto
S
S
Tatsumi
K
K
Mino
M
M
Yagi
K
K
D000818:Animals; D004110:Diltiazem / Q000494:pharmacology; D004317:Doxorubicin / Q000378:metabolism* / Q000494:pharmacology; D004351:Drug Resistance; D007941:Leukemia P388 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D013050:Spectrometry, Fluorescence; D013629:Tamoxifen / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014750:Vincristine / Q000378:metabolism / Q000494:pharmacology
D013629:Tamoxifen; D014750:Vincristine; D004317:Doxorubicin; D004110:Diltiazem