title: Human recipient cell for oncogene transfection studies.
abstract: We used human oncogene DNA to transform the nontumorigenic, revertant, human osteosarcoma cell line HOS TE-85 clone 5 (ATCC CRL 1543) to tumorigenicity in athymic nude mice with latency periods as short as 3 weeks. These cells were also transformed by genetic markers in genomic DNA samples. Because of their low rate of spontaneous tumor formation and the simplicity of culturing them, HOS cells provide a human cell alternative to NIH 3T3 murine fibroblasts for oncogene transfection studies.
authors.forename: M A
authors.initials: MA
authors.lastname: Tainsky
authors.forename: F L
authors.initials: FL
authors.lastname: Shamanski
authors.forename: D
authors.initials: D
authors.lastname: Blair
authors.forename: G
authors.initials: G
authors.lastname: Vande Woude
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009857:Oncogenes; D012516:Osteosarcoma / Q000235:genetics; D011519:Proto-Oncogenes; D014162:Transfection
chemical_list: D004247:DNA