title: Human serum-dependent survival of GM-CFCs in vitro from patients with chronic granulocytic leukemia.
abstract: Bone marrow (BM) cells from 15 patients with chronic granulocytic leukemia (CGL) and 12 normal donors were placed in liquid culture for 4-7 days in the presence of fetal calf serum (FCS) or human AB serum. BM cells were plated in agar for GM-CFC growth at day 0, day 4, day 7 of culture, in the presence of human placenta conditioned medium (HPCM). The recovery of GM-CFCs on day 4 in FCS was 86 +/- 18 and 15 +/- 18% from normal or CGL BM cells respectively (p = 0.005). The recovery of GM-CFCs on day 4 in human AB serum was comparable for normal (66 +/- 48%) and CGL (69 +/- 32%) BM cells. Similar results were obtained on day 7 of culture. Cytochemical staining of agar plates showed a sharp drop of macrophage colonies in CGL BM cells kept in FCS cultures on day 4-7, when compared to both normal BM cells and baseline colonies. These data suggest that the survival in liquid culture of GM-CFCs from patients with CGL is dependent on a factor present in human and not in fetal calf serum. This is not the case for normal GM-CFCs.
authors.forename: F
authors.initials: F
authors.lastname: Frassoni
authors.forename: M
authors.initials: M
authors.lastname: Repetto
authors.forename: G
authors.initials: G
authors.lastname: Piaggio
authors.forename: M
authors.initials: M
authors.lastname: Podestà
authors.forename: A M
authors.initials: AM
authors.lastname: Marmont
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D006133:Growth Substances / Q000097:blood; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D008264:Macrophages / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010920:Placenta / Q000502:physiology; D013045:Species Specificity
chemical_list: D003470:Culture Media; D006133:Growth Substances