Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs.
The leaf disc transformation system provides a simple means to score expression of various T-DNA markers within days of infection by Agrobacterium tumefaciens as well as long-term selection for growth of transformed callus and shoots. In this report, we describe the application of this system to evaluation of marker transfer and integration in a comprehensive set of defined avirulent mutants of A. tumefaciens. We conclude that virC is not essential when the T-DNA is present on a binary vector. The most likely explanation for this is that the virC gene product is involved in generation of a T-DNA intermediate.
Horsch
R B
RB
Klee
H J
HJ
Stachel
S
S
Winans
S C
SC
Nester
E W
EW
Rogers
S G
SG
Fraley
R T
RT
D001120:Arginine / Q000031:analogs & derivatives / Q000235:genetics; D002478:Cells, Cultured; D009154:Mutation; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics / Q000472:pathogenicity*; D014170:Transformation, Genetic
C008666:nopaline; C005394:octopine; D001120:Arginine