DNA aneuploidia in cells of acute leukemia non B non T type in children.
In 31 children with acute lymphoblastic leukemia of non B and non T type the cellular DNA amount using cytophotometric method was estimated. Parallely the computer microscopic image analysis was performed. These investigations were made using Morphoquant of C. Zeiss, Jena, GDR. The cells were studied before the introduction of therapy, after obtaining the first phase remission, during remission and at the relapse. DNA-diploidal type in 13 cases, aneuploidio-poliploidal in 8 cases and hipoploidal type in 13 cases. The survival time of investigated children was the largest in the case with diploidal and hyperploidal types of leukemic cells. The children with DNA-aneuploidopoliploidal type cells had the shortest survival time. Besides the estimation of the cellular DNA amount the defining of the percentage of the cells being in S phase seems to be very important. In differentiating between the normal and leukemic lymphocytes, containing the same amount of DNA as non neoplastic lymphocytes, it may be advisable to analyse the morphological picture, particularly of such parameters as chromatin compactness degree and the presence of nucleoli.
Krygier-Stojaøowska
A
A
Zwierzykowska
A
A
Rakowska-Plusser
M
M
Urasiński
T
T
Osipowicz
E
E
Gapski
Z
Z
Woźniak
B
B
D000782:Aneuploidy; D002453:Cell Cycle; D002466:Cell Nucleolus / Q000648:ultrastructure; D002648:Child; D002843:Chromatin / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011379:Prognosis
D002843:Chromatin; D004273:DNA, Neoplasm