title: [Measurement of total bile acids. Comparison of different preparations of 3 alpha-hydroxysteroid dehydrogenase].
abstract: Oxidation of free and conjugated biliary acids was carried out with 5 different enzymatic preparations of 3 alpha-hydroxysteroid dehydrogenase. The comparison of the catalytic activities obtained, shows the existence of important variations according to the enzymes. Preparations purified by chromatography have a stronger activity toward primary biliary acids than toward secondary biliary acids, while preparations obtained from bacterial mutants have a low activity toward cholic acid and its conjugates.
authors.forename: D
authors.initials: D
authors.lastname: Feldmann
authors.forename: A
authors.initials: A
authors.lastname: Beaudonnet
authors.forename: J
authors.initials: J
authors.lastname: Goudable
authors.forename: B
authors.initials: B
authors.lastname: Petigny
authors.forename: H
authors.initials: H
authors.lastname: Sarmini
authors.forename: C
authors.initials: C
authors.lastname: Vercambre
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000302:isolation & purification; D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis* / Q000097:blood; D002384:Catalysis; D006801:Humans; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000201:enzymology
chemical_list: D001647:Bile Acids and Salts; D015096:3-Hydroxysteroid Dehydrogenases
vernacular_title: Dosage des acides biliaires totaux. Comparaison de différentes préparations de 3 alpha-hydroxystéroïde déshydrogénase.