Rearrangement and enhanced expression of c-myc oncogene in fresh tumor cells obtained from a patient with acute lymphoblastic leukemia.
Both enhanced and altered expressions of cellular oncogenes have recently been implicated in specific forms of human malignancy (for review: [2, 8]). The fact that certain human tumors have characteristic chromosomal translocations has led to the hypothesis that specific cellular oncogenes may be 'activated' in these genetic recombinations. In a number of human undifferentiated B-cell lymphoma (UBL) cell lines carrying the t(8:14) translocation it has been shown that the human c-myc gene is located on the region of chromosome 8 (8q24), which is translocated to the immunoglobulin heavy-chain (IHC) locus on chromosome 14 [7, 24]. We report here the molecular cloning of the recombination site between c-myc and IHC in fresh uncultured cells obtained from a patient with rapidly progressive and fatal acute lymphoblastic leukemia. The translocation was associated with an enhanced c-myc expression in the tumor cells of this patient.
Blick
M
M
Westin
E
E
Wong-Staal
F
F
Gallo
R
R
McCredie
K
K
Gutterman
J
J
D001483:Base Sequence; D002874:Chromosome Mapping; D002901:Chromosomes, Human, 13-15; D002906:Chromosomes, Human, 6-12 and X; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics*; D009857:Oncogenes; D011995:Recombination, Genetic; D014178:Translocation, Genetic