Characterization and mobilization of nonconjugative plasmids encoding resistance to streptomycin and sulfanilamide.
Most of nonconjugative streptomycin (Sm)- and sulfanilamide (Su)- resistance of clinical isolates belonging to various species of Enterobacteriaceae and Pseudomonas were encoded by an Inc Q plasmid, molecular size of which was 5.5 Md. The SmSu plasmids were efficiently mobilized by Inc P plasmids between E. coli strains. Inc I group and Inc F group plasmids could mobilize the Inc Q plasmids at lower efficiencies. The Inc Q plasmid was also mobilized to various species of Enterobacteriaceae at high frequencies without accompanying the conjugative Inc P plasmid; as a result, most of the SmSu-resistant transconjugants were nontransferable. The above results may explain the wide distribution of nonconjugative SmSu strains among clinical isolates.
Araki
Y
Y
Laboratory of Drug Resistance in Bacteria, Gunma University School of Medicine.
Inoue
M
M
Hashimoto
H
H
D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000235:genetics*; D011815:R Factors; D013307:Streptomycin / Q000494:pharmacology*; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000494:pharmacology*
D013424:Sulfanilamides; D000077145:Sulfanilamide; D013307:Streptomycin