A kinetic analysis of peritoneal fluid cytology and arachidonic acid metabolism after abrasion and reabrasion of rabbit peritoneum.
The purpose of this study was to investigate the cellular composition of peritoneal fluid during post-surgical re-epithelialization and to determine the metabolism of arachidonic acid by these cells. Rabbits underwent a midline laparotomy followed by abrasion of the broad ligament. The presence of adhesions was graded and the peritoneal exudative cells collected up to 14 days thereafter. Ascitic fluid and cells were separated by centrifugation and the cellular percipitate incubated with [14C]arachidonic acid. The aliquot was separated by silica Gel G thin-layer chromatography and the specific radioactivity of each strip determined. To evaluate the reformation of adhesions 14 days after the first abrasion, rabbits underwent reabrasion of the same area and the pattern of arachidonic acid metabolism by the ascites cells was similarly evaluated. Six hours after the abrasion, PMNs comprised 87.5% of the peritoneal exudative cells (total 0.03 X 10(7) cells/rabbit). On Day 3, the total cell number increased to 2.92 X 10(7), 97.6% of which were large mononuclear cells. No significant change in the type of distribution of adhesions was evident from 6 hr through Day 2. After Day 7, the total number of adhesions was minimal; however, those that were present were primarily severe. The second-look evaluation of adhesion formation was not found to be consistent prior to the 7th postoperative day, since many filmy bands present prior to that time were not present later. The in vitro formation of 5-HETE by these cells increased from 6 hr through Day 11. Production of di-HETE increased beginning Day 3 and maintained high steady state levels thereafter.(ABSTRACT TRUNCATED AT 250 WORDS)
Shimanuki
T
T
Nakamura
R M
RM
diZerega
G S
GS
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001202:Ascitic Fluid / Q000166:cytology*; D015232:Dinoprostone; D005260:Female; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism; D010537:Peritoneum / Q000601:surgery*; D011458:Prostaglandins E / Q000378:metabolism; D011817:Rabbits; D013929:Thromboxane B2 / Q000378:metabolism; D014945:Wound Healing
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D011458:Prostaglandins E; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D013929:Thromboxane B2; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D015232:Dinoprostone