A preliminary three-dimensional structure of angiogenin.
A preliminary three-dimensional structure of angiogenin has been computed, based on its homology to bovine pancreatic ribonuclease A. A standard-geometry structure of ribonuclease was first obtained from its x-ray coordinates. The fit of the backbone of angiogenin to that of ribonuclease was then optimized by taking account of amino acid deletions and by minimizing its conformational energy-plus-a-penalty distance function constraining its backbone to that of ribonuclease. Side-chain and backbone dihedral angles were allowed to vary throughout the cycles of energy minimization. In the last stages of minimization, the penalty distance function was removed. A low-energy structure resembling ribonuclease was obtained.
Palmer
K A
KA
Scheraga
H A
HA
Riordan
J F
JF
Vallee
B L
BL
D006801:Humans; D008958:Models, Molecular; D009363:Neoplasm Proteins; D011487:Protein Conformation; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases; D012984:Software; D013816:Thermodynamics
D009363:Neoplasm Proteins; D012260:Ribonucleases; C046366:angiogenin; D012259:Ribonuclease, Pancreatic