DNA-mediated gene transfer into epidermal cells using electroporation.
A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB/c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10(-6) cells per microgram of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones.
Reiss
M
M
Jastreboff
M M
MM
Bertino
J R
JR
Narayanan
R
R
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D004560:Electricity; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D051379:Mice; D013997:Time Factors; D014162:Transfection
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase