Quantitative study of liver metastases from colon cancer in rats after treatment with cyclosporine A.
Liver metastases were produced in syngeneic BD IX rats by intraportal injection of colon cancer cell aggregates. The cells originated from the DHD/K12 cell line, derived from a 1,2-dimethylhydrazine (CAS: 540-73-8)-induced colon adenocarcinoma in BD IX rats. The animals received either cyclosporine A (CSA) or the excipients alone (control) through daily gastric intubation during 6 weeks. Multiple and very large hepatic metastases were observed early in 100% of the CSA-treated rats. The mean tumor volume was approximately 2,000 times higher in the CSA-treated group than in the controls (P less than .01). Survival time in the CSA-treated group was shortened (P less than .01) by generalized metastatic disease. Easy production of metastasis from colon cancer in 100% of the animals and precise estimation of tumor volume may prove useful for future therapeutic studies of secondary hepatic disease.
van der Elst
J
J
De Greve
J
J
Geerts
F
F
De Neve
W
W
Storme
G
G
Willems
G
G
D000230:Adenocarcinoma / Q000517:prevention & control / Q000556:secondary*; D000818:Animals; D001835:Body Weight; D002460:Cell Line; D003110:Colonic Neoplasms; D003201:Computers; D003524:Cyclosporins / Q000627:therapeutic use*; D008113:Liver Neoplasms / Q000517:prevention & control / Q000556:secondary*; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D009929:Organ Size; D010534:Peritoneal Neoplasms / Q000556:secondary; D011169:Portal Vein; D051381:Rats; D013997:Time Factors
D003524:Cyclosporins