Syntactic pattern analysis of 5'-splice site sequences of mRNA precursors in higher eukaryote genes.
The signals which direct the excision of introns from eukaryotic pre-mRNA are not yet well understood. In order to define the signals for 5'-splice sites of mRNA splicing, nucleotide sequences including 5'-splice junctions of mammalian pre-mRNAs are analysed by means of syntactic pattern analysis. Taking this approach, we infer the grammatical rules which specify 5'-splice sites and construct a finite automaton which is the recognizer of the nucleotide sequences at 5'-splice sites. By scanning the automaton along nucleotide sequences, we can identify the positions of 5'-splice junctions with a degree of discrimination of up to 94-97% in the known genes, while the degree of prediction is in the range 50-55% in new genes.
Kudo
M
M
Division of Information Engineering, Graduate School of Engineering, Hokkaido University, Sapporo, Japan.
Iida
Y
Y
Shimbo
M
M
D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D005057:Eukaryotic Cells; D006801:Humans; D007438:Introns; D010363:Pattern Recognition, Automated; D012322:RNA Precursors; D012326:RNA Splicing
D012322:RNA Precursors