Hemosorption of restored long-preserved blood.
Incubation of long-preserved donor blood erythrocytes (over 21 days) with rejuvenating solution Erythropifaden resulted in an increased ATP concentration to 2.03 +/- 0.34 mmol/l; increased 2,3-DPG to 3.89 +/- 0.51 mmol/l, and the number of erythrocyte discoid forms was raised to 30%. Further blood perfusion through SKN-D hemosorbent allowed to reduce the following adenine level to zero; riboxine by 90; citrate and lactate by 50%. The plasma K+ was decreased from 29.8 +/- 1.25 to 13.0 +/- 0.87 mmol/l, while blood pH rose from 6.675 +/- 0.018 to 7.310 +/- 0.028. At the end of the perfusion, the ATP level was seen to further increase to 2.53 +/- 0.28 mmol/l, and 2,3-DPG to 4.99 +/- 0.7 mmol/l. The indices of osmotic resistance and deformability of erythrocytes were normalized. The discocyte number reached 40%. The amount of red blood cell irreversible forms declined from 26 to 13% without any increment plasma free hemoglobin. The data obtained confirm the high efficacy of the combination of the rejuvenating and hemosorption procedures when applied to stored long-preserved blood.
Agronenko
V A
VA
Kavetsky Institute for Oncology Problems, AS Akr. SSR, Kiev.
Belkin
A L
AL
Tibilova
N N
NN
Pendrak
K A
KA
Povzhitkova
M S
MS
Petrenko
S V
SV
Tsisarenko
T A
TA
Markova
N A
NA
Platonova
O V
OV
Ermolchuk
O N
ON
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D006464:Hemoperfusion; D006801:Humans; D066298:In Vitro Techniques; D013997:Time Factors; D014118:Toxins, Biological / Q000097:blood / Q000302:isolation & purification
D004163:Diphosphoglyceric Acids; D014118:Toxins, Biological; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate