Immune clearance of liposomes inhibited by an anti-Fc receptor antibody in vivo.
In a study designed to evaluate the potential for in vivo manipulation of the circulation and tissue distribution of injected liposomes, mice were passively injected with antidinitrophenyl (anti-DNP) monoclonal antibodies of the IgG2a or IgG2b subclasses or were immunized with the nitrophenyl hapten bound to a protein carrier. They were then injected i.v. with 125I- and carboxyfluorescein-labeled, DNP-bearing liposomes. Circulation time of the DNP-bearing liposomes was markedly reduced in actively and passively immune mice, with increased deposition of liposomes in the liver. The increased clearance of liposomes could be abrogated by injection of a monoclonal antibody directed against the murine IgG Fc receptor (2.4G2). The results suggest that clearance of ligand-bearing reagent in the face of an immune response may be modified by specific immunologic manipulation in vivo.
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D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004136:Dinitrobenzenes / Q000276:immunology; D007116:Immunization, Passive; D008081:Liposomes / Q000276:immunology* / Q000378:metabolism; D008657:Metabolic Clearance Rate; D051379:Mice; D010587:Phagocytosis; D011961:Receptors, Fc / Q000276:immunology*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004136:Dinitrobenzenes; D008081:Liposomes; D011961:Receptors, Fc