title: Lack of sampling site variation in chorion villus biopsy as assessed by DNA, enzymatic, morphological and cytogenetical analyses.
abstract: A study of villus samples from eight random sites on five electively aborted chorion sacs was performed to determine any significant differences in yield, quality and composition of DNA, iduronate sulphate sulphatase activity and karyotype status. The villi were also examined for their histological characteristics (e.g. stem, intermediate or terminal villi) and for HCG and BGP immuno-reactivities. The overall findings indicated no significant site to site variations in any of the parameters studied. It is therefore proposed that any villus should be equally suitable for prenatal diagnosis.
authors.forename: M
authors.initials: M
authors.lastname: Upadhyaya
authors.forename: B
authors.initials: B
authors.lastname: Jasani
authors.forename: E
authors.initials: E
authors.lastname: Little
authors.forename: P S
authors.initials: PS
authors.lastname: Harper
authors.forename: D
authors.initials: D
authors.lastname: Rees
authors.forename: A
authors.initials: A
authors.lastname: Roberts
mesh_terms: D002823:Chorion / Q000166:cytology*; D006063:Chorionic Gonadotropin / Q000032:analysis; D002824:Chorionic Villi / Q000201:enzymology / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D007066:Iduronate Sulfatase / Q000378:metabolism; D007621:Karyotyping; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis; D011296:Prenatal Diagnosis
chemical_list: D006063:Chorionic Gonadotropin; D011257:Pregnancy Proteins; D004247:DNA; D007066:Iduronate Sulfatase