title: Structural organization of the genome of the zygomycete Absidia glauca: evidence for high repetitive DNA content.
abstract: Total cell DNA of Absidia glauca has a GC-content of 44.6% +/- 0.5% as determined from optical melting profiles which is in good accordance with values from equilibrium centrifugation in bisbenzimide containing CsCl gradients (46.2% +/- 1.1%), whereas mitochondrial DNA has a GC-content of only 30%. The genome size of Absidia glauca is approximately 36,000 kb, 8.6 times that of Escherichia coli. Three kinetically different fractions could be identified in reassociation experiments: a foldback-DNA fraction, comprising approximately 10% of the total DNA, repetitive DNA (25%) and single copy DNA (65%). This relatively high amount of repetitive DNA could partly be ascribed to ribosomal DNA (13%) and a new interspersed repetitive element ("rAg1") which has been cloned in pBR325.
authors.affiliation: Institut für Biochemie und Molekulare Biologie/Botanik, Technische Universität Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Wöstemeyer
authors.forename: A
authors.initials: A
authors.lastname: Burmester
mesh_terms: D001482:Base Composition; D004271:DNA, Fungal / Q000235:genetics; D005658:Fungi / Q000235:genetics*; D005800:Genes, Fungal; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal; D012335:RNA, Ribosomal