[Adenylate cyclase of the causative agent of plague: its purification and properties].
Three forms of adenylate cyclase have been detected in Y. pestis: membrane-bound, cytoplasmic and extracellular. Extracellular adenylate cyclase has been purified so as to achieve a homogeneous state, and some of its physicochemical parameters have been investigated. In the process of purification the initial preparation of this enzyme has been subjected to heating at 100 degrees C for 15 minutes, fractionation with ammonium sulfate, and gel filtration on Sephadex G-100. The homogeneity of adenylate cyclase has been confirmed by electrophoresis in 7.5% polyacrylamide gel and precipitation by the plague agglutinating serum. The enzyme has been found to have a molecular weight of 30,000 daltons and to show the optimum activity at pH 7.0-7.2 and at a temperature between 37 and 40 degrees C. Monospecific rabbit serum to the homogeneous preparation of adenylate cyclase has been obtained.
Shevchenko
L A
LA
Mishankin
B N
BN
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D008970:Molecular Weight; D011817:Rabbits; D013696:Temperature; D015010:Yersinia pestis / Q000201:enzymology* / Q000276:immunology
D000067956:Adenylyl Cyclase Inhibitors; D000942:Antigens, Bacterial; D000262:Adenylyl Cyclases
Adenilattsiklaza vozbuditelia chumy: ochistka i nekotorye svoĭstva.