Transforming growth factor type beta regulation of actin mRNA.
Stimulation of quiescent cultures of AKR-2B cells with transforming growth factor type beta (TGF beta) resulted in a transitory increase in actin cytoplasmic poly(A) + RNA. Levels of actin mRNA peaked approximately 4-8 hours subsequent to TGF beta addition and approached basal levels by 24 hours. The accumulation of actin transcripts was dose dependent and insensitive to inhibitors of protein synthesis; 1-3 ng/ml TGF beta induced maximal actin gene expression. Actin isotype-specific probes demonstrated that both beta- and gamma-cytoplasmic actins are induced by TGF beta.
Leof
E B
EB
Proper
J A
JA
Getz
M J
MJ
Moses
H L
HL
D008841:Actin Cytoskeleton / Q000502:physiology; D000199:Actins / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D015291:Transforming Growth Factors
D000199:Actins; D006133:Growth Substances; D010455:Peptides; D012333:RNA, Messenger; D015291:Transforming Growth Factors; D003513:Cycloheximide