The oxidation product of molybdenum cofactor from milk xanthine oxidase.
In extracts of acid treated molybdenum cofactor containing xanthine oxidase, fluorescence is maximally developed upon a three hours incubation. Analysis by means of reversed phase HPLC revealed the presence of several fluorescent compounds, the main one being a blue fluorescent compound with an emission maximum of 465 nm when maximal excited at 395 nm at a neutral pH. Definite proof is presented that this compound is the oxidation product of the molybdenum cofactor. The remaining fluorescent products are shown to be pterin-derivatives, yielding predominantly pterin-6-carboxylic acid upon permanganate oxidation. Purified oxidation product of molybdenum cofactor however, didn's yield a fluorescent derivative at all upon treatment with permanganate.
van Spanning
R J
RJ
Free University, Department of Microbiology, Amsterdam, The Netherlands.
Wansell-Bettenhaussen
C W
CW
Oltmann
L F
LF
Stouthamer
A H
AH
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003067:Coenzymes; D008667:Metalloproteins / Q000032:analysis*; D008892:Milk / Q000201:enzymology*; D000090006:Molybdenum Cofactors; D010084:Oxidation-Reduction; D011621:Pteridines / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis; D013997:Time Factors; D014969:Xanthine Oxidase / Q000032:analysis*
D003067:Coenzymes; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D011621:Pteridines; D013438:Sulfhydryl Compounds; D014969:Xanthine Oxidase; C022261:molybdopterin