[14C]chloroacetylcholine as an advantageous affinity label of the acetylcholine receptor.
The alkylating agent [14C]chloroacetylcholine perchlorate [( 14C] ClACh) was synthesized and used for affinity labelling of the nicotinic acetylcholine receptor from Torpedo marmorata. Solubilized and affinity-purified receptor proteins were reduced and alkylated according to the bromoacetylcholine-method. Covalent binding of [14C] ClACh to the cholinergic receptor proved to be specific and saturable, and occurred exclusively to the alpha-subunit. Halogen substitution of acetylcholine by chlorine and insertion of a 14C-isotope instead of the widely used 3H resulted in favourable properties of the affinity label.
Bodmer
D M
DM
Department of Pharmacology, University of Zurich, Switzerland.
Sin-Ren
A C
AC
Waser
P G
PG
D000109:Acetylcholine / Q000031:analogs & derivatives*; D000345:Affinity Labels; D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D007553:Isotope Labeling; D011950:Receptors, Cholinergic / Q000032:analysis*; D014101:Torpedo
D000345:Affinity Labels; D002250:Carbon Radioisotopes; D011950:Receptors, Cholinergic; D000109:Acetylcholine