Encapsulation of thiosulfate: cyanide sulfurtransferase by mouse erythrocytes.
Murine carrier erythrocytes, prepared by hypotonic dialysis, were employed in the encapsulation of several compounds including [14C]sucrose, [3H]inulin, and bovine thiosulfate:cyanide sulfurtransferase (rhodanese), a mitochondrial enzyme which converts cyanide to thiocyanate. Approximately 30% of the added [14C]sucrose, [3H]inulin, and rhodanese was encapsulated by predialyzed erythrocytes, and a decrease in the mean corpuscular volume and mean corpuscular hemoglobin was observed. In the encapsulation of rhodanese a recovery of 95% of the erythrocytes was achieved and an 85% equilibrium was established. The addition of potassium cyanide (50 mM) to intact, rhodanese-loaded erythrocytes containing sodium thiosulfate resulted in its metabolism to thiocyanate. These results establish the potential use of erythrocytes as biodegradable drug carrier in drug antagonism.
Leung
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Ray
L E
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Sander
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C
Way
J L
JL
Sylvester
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DM
Way
J L
JL
D000818:Animals; D002250:Carbon Radioisotopes; D003486:Cyanides / Q000378:metabolism*; D003956:Dialysis; D004912:Erythrocytes / Q000201:enzymology*; D008658:Inactivation, Metabolic; D007444:Inulin / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009994:Osmolar Concentration; D013395:Sucrose / Q000097:blood; D013466:Sulfurtransferases / Q000097:blood*; D013884:Thiosulfate Sulfurtransferase / Q000008:administration & dosage / Q000097:blood*; D014316:Tritium
D002250:Carbon Radioisotopes; D003486:Cyanides; D014316:Tritium; D013395:Sucrose; D007444:Inulin; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase