title: Some histochemical and ultrastructural observations on the early foetal pig testis.
abstract: Testes of foetal pigs between 26 to 35 days post coitum (p.c.) were investigated histochemically and ultrastructurally. Diaphorase and delta 5-3 beta-hydroxysteroid dehydrogenase activities were studied using, respectively, NADH and pregnenolone and dihydroxy androsterone as substrates. Ultrastructurally, attention was focused on the development of mesenchymal cells and on the sustentacular cells in the primitive sex cords in an attempt to detect the origin of Leydig cells. Histochemically there is a concentration of activity toward the interstitium with increasing age. Also the reactions increase in intensity. Ultrastructurally no evidence for Leydig cell development from Sertoli cells could be observed. Mesenchymal cells between the sex cords show a development toward Leydig cells. This is absent in mesenchymal cells in the future tunica albuginea. Before 30 days p.c. no 'true' Leydig cells can be observed morphologically. The role of the rough endoplasmic reticulum/mitochondrial complex, which is present in many mesenchymal and sustentacular cells, is discussed.
authors.forename: C J
authors.initials: CJ
authors.lastname: van Vorstenbosch
authors.forename: C M
authors.initials: CM
authors.lastname: van Rossum-Kok
authors.forename: B
authors.initials: B
authors.lastname: Colenbrander
authors.forename: C J
authors.initials: CJ
authors.lastname: Wensing
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis; D000818:Animals; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis; D005333:Fetus / Q000648:ultrastructure*; D005854:Germ Cells / Q000648:ultrastructure; D006651:Histocytochemistry; D007985:Leydig Cells / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D012708:Sertoli Cells / Q000648:ultrastructure; D013552:Swine; D013737:Testis / Q000648:ultrastructure*
chemical_list: D015096:3-Hydroxysteroid Dehydrogenases; D008058:Dihydrolipoamide Dehydrogenase