An X-ray analysis of the orthorhombic crystal form of trichosanthin at 5 angstrom.
Trichosanthin crystals grown at pH 5.4 are orthorhombic P212121 with cell dimension a = 38.23 angstrom, b = 76.18 angstrom and c = 79.12 angstrom. Each asymmetric unit contains one protein molecule of 234 amino acid residues (M. W. 26,000). The structure has been solved at 5 angstrom resolution by conventional MIR method with two heavy-atom derivatives. The preliminary result suggests that the protein molecule in acidic solution does not have dramatic conformational change, compared with that in alkali solution.
Ma
X Q
XQ
Institute of Biophysics, Academia Sinica, Beijing.
Wang
Y P
YP
Wang
J H
JH
D000019:Abortifacient Agents / Q000032:analysis*; D000020:Abortifacient Agents, Nonsteroidal / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*; D011487:Protein Conformation; D015978:Trichosanthin; D014961:X-Ray Diffraction
D000019:Abortifacient Agents; D000020:Abortifacient Agents, Nonsteroidal; D010940:Plant Proteins; D015978:Trichosanthin