Absorption and transport of radioactive tracers in the midgut of the malaria mosquito, Anopheles stephensi.
Three radiolabeled substances were mixed with fresh heparinized mouse blood and fed to female Anopheles stephensi through a chicken crop membrane. Resorption and transport in the anterior (A) and posterior (P, stomach) parts of the midgut were investigated by means of electron microscopic autoradiography. Digestion products of 125I-labeled bovine serum albumin (BSA) were resorbed only in the P-part, showing a biphasic pattern with maximal values 4 and 18 hr after feeding. Uptake of 3H-labeled amino acids started immediately after feeding in both midgut parts. [14C]glucose was mainly absorbed in the P-part. Labeled carbohydrate storage deposits formed during the initial phase of digestion and were mobilized around 36 hr after feeding. After feeding the mosquitoes on 125I-BSA, oocyte protein yolk spheres and abdominal cuticle became labeled. Ingestion of 3H-labeled amino acids caused the formation of silver grains over follicles and fat body lipids. Feeding on [14C]glucose resulted in labeled fat body carbohydrates and oocyte protein yolk spheres.
Schneider
M
M
Swiss Tropical Institute, Basel, Switzerland.
Rudin
W
W
Hecker
H
H
D000818:Animals; D000852:Anopheles / Q000378:metabolism* / Q000648:ultrastructure; D004064:Digestive System / Q000378:metabolism / Q000648:ultrastructure; D007408:Intestinal Absorption; D007457:Iodine Radioisotopes; D008854:Microscopy, Electron; D012710:Serum Albumin, Bovine / Q000378:metabolism
D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine