The 21-acetylation of corticosteroids by Clostridium sporogenes.
A strain of Clostridium sporogenes, an anaerobic bacterium, isolated from sewage in New York City synthesizes two constitutive enzymes with action on steroid molecules: (i) an enzyme capable of selectively acetylating the 21-hydroxyl function of certain steroids and (ii) the corresponding esterase. Under our experimental conditions the enzymes have a strict structural requirement for 3-keto-4-ene and C-20-keto or 20 alpha-hydroxyl group and convert their respective substrates to a mixture of free and acetylated products.
Winter
J
J
Department of Pathology, St. Luke's-Roosevelt Hospital Center, New York, NY 10025.
Shackleton
C H
CH
Morris
G N
GN
Locascio
S
S
Bokkenheuser
V D
VD
D000107:Acetylation; D000305:Adrenal Cortex Hormones / Q000378:metabolism*; D000446:Aldehyde-Lyases / Q000378:metabolism; D000693:Anaerobiosis; D002855:Chromatography, Thin Layer; D003013:Clostridium / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013254:Steroid 17-alpha-Hydroxylase; D013379:Substrate Specificity
D000305:Adrenal Cortex Hormones; D009580:Nitroblue Tetrazolium; D003577:Cytochrome P-450 Enzyme System; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases