Effect of lithium on circadian activity of AChE (EC 3.1.1.7) in the reticular formation of mouse brain stem under LD 12:12.
The investigation was carried out on male mice 6-8 weeks old. Control animals were fed standard chow without lithium, while the experimental ones received on additional 10 mmol LiCl/kg of chow. After 30 days feeding, 7 mice at a time were killed every 4h. The activity of free AChE was assessed in the brain stem reticular formation according to the method of Ellman. The results were statistically analyzed using Student's t-test and Fisher test. Parameters of periodic function were then applied to the means obtained by the method of least-squares. In both the control and experimental groups diel changes in the AChE activity were observed. Acrophase activity occurred in the control group at 1956. In the experimental group lithium caused a shift in acrophase to 0551. It may therefore be supposed that lithium not only affects the duration of the rhythm period but also shifts its phase by 1800. This could be relevant to the therapeutic use of lithium in manic-depressive patients who may present advancing phase of circadian rhythms.
Lewandowski
M H
MH
Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland.
Mardyła
E
E
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D008094:Lithium / Q000494:pharmacology*; D008297:Male; D051379:Mice; D012154:Reticular Formation / Q000201:enzymology*
D008094:Lithium; D000110:Acetylcholinesterase