title: Transformation as a tool for studying the epidemiology of tet determinants in Streptococcus pneumoniae.
abstract: Transformation of pneumococcus was used to detect homology among tetracycline resistance determinants of clinical isolates of Streptococcus pneumoniae. A strain of pneumococcus containing a mutated tet determinant (tet-3), of class M, integrated into the chromosome was used as a recipient in transformation experiments, where donor DNA was from the tetracycline resistant isolates. 34/34 strains appeared to have tet determinants homologous to tet-3 (i.e. tet M). Still using transformation it was possible to determine that the tet-3 transforming activity of DNA from Tn916 and S. pneumoniae BM6001 was contained in a 5 kb HincII fragment. For this purpose a transformation technique where donor DNA was directly taken from low melting point agarose gels was standardized and used.
authors.forename: G
authors.initials: G
authors.lastname: Pozzi
authors.forename: M
authors.initials: M
authors.lastname: Stellini
authors.forename: L
authors.initials: L
authors.lastname: Marri
authors.forename: A M
authors.initials: AM
authors.lastname: Molina
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005819:Genetic Markers; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology*; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers; D013752:Tetracycline