A catecholic 9,10-seco steroid as a product of aerobic catabolism of cholic acid by a Pseudomonas sp.
A mutant of the efficient bile acid-utilizing Pseudomonas putida ATCC 31752 was found to accumulate three major catabolites on aerobic growth on cholic acid. One of these catabolites was isolated and identified as 3,4,7,12 beta-tetrahydroxy-9,10-seco-1,3,5(10)-androstatriene-9,17-dione (2). This is the first catecholic 9,10-secosteroid isolated from the microbial degradation of bile acids or sterols and confirms the role of such secosteroids in the microbial degradative pathway for steroids.
Park
R J
RJ
CSIRO Division of Food Research, Meat Research Laboratory, Queensland, Australia.
Dunn
N W
NW
Ide
J A
JA
D000332:Aerobiosis; D000733:Androstatrienes / Q000032:analysis / Q000378:metabolism*; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism*; D002850:Chromatography, Gel; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D011549:Pseudomonas / Q000378:metabolism*; D012632:Secosteroids / Q000032:analysis / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D000733:Androstatrienes; D002793:Cholic Acids; D012632:Secosteroids; C055280:3,4,7,12-tetrahydroxy-9,10-seco-1,3,5(10)-androstatriene-9,17-dione; D019826:Cholic Acid