Phenotypic changes associated with chemically induced differentiation of a hairy cell leukemia cell line.
In vitro chemically induced differentiation of the JOK-1 cell line, derived from a patient with hairy cell leukemia, has been studied. Non-induced JOK-1 cells expressed sIgM, B-1, CALLA, Ia, and C3 receptors. Following exposure to 2% DMSO, or 100 ng/ml 12-O-tetradecanoyl-phorbol-13-acetate (TPA), the cells underwent a phenotypic change from lymphoblastoid to monocytoid. In the presence of TPA, they became adherent and developed dendritic processes. Associated with these changes was a decrease in sIgM and CALLA and variable expression of C3 receptors. No monocyte/macrophage-associated antigens were detected. These results suggest that the JOK-1 cell line may have a limited ability to differentiate but is not mature enough to complete the differentiation process.
Hooper
W C
WC
Barth
R F
RF
Minowada
J
J
D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003582:Cytogenetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000276:immunology / Q000473:pathology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis; D010641:Phenotype; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide