title: Three-step procedure for isolation of epithelial cells from the lungs of sheep with jaagsiekte.
abstract: An efficient and reproducible technique is described for the isolation of transformed sheep pulmonary adenomatosis cells. It includes three basic steps: prolonged trypsinisation to kill fibroblasts, magnetic removal of macrophages and adherence to remove the rapidly adherent cells. The resultant preparations of lung cells were enriched to 96.6 per cent type 2 pneumocytes.
authors.affiliation: Moredun Research Institute, Edinburgh.
authors.forename: F A
authors.initials: FA
authors.lastname: Jassim
authors.forename: J M
authors.initials: JM
authors.lastname: Sharp
authors.forename: P D
authors.initials: PD
authors.lastname: Marinello
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004848:Epithelium / Q000473:pathology; D008168:Lung / Q000473:pathology*; D011648:Pulmonary Adenomatosis, Ovine / Q000473:pathology*; D012756:Sheep