High frequency of N-ras activation in acute myelogenous leukemia.
Using the NIH/3T3 cell transfection assay, activated cellular oncogenes have been detected in around 10% to 20% of human tumors. From a series of DNA preparations from tissues infiltrated with acute myelogenous leukemia (AML), 50% (3/6) caused transformation of NIH/3T3 cells. Thus AML appears to be the human tumor with the highest frequency of oncogenes detected by DNA transfection. In each case the oncogene involved was N-ras, a member of the ras gene family. Biologic and clinical parameters of AML patients with and without N-ras oncogenes in their tumors are discussed.
Needleman
S W
SW
Kraus
M H
MH
Srivastava
S K
SK
Levine
P H
PH
Aaronson
S A
SA
D000293:Adolescent; D000818:Animals; D002869:Chromosome Aberrations; D005260:Female; D005784:Gene Amplification; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D051379:Mice; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011519:Proto-Oncogenes; D011995:Recombination, Genetic; D014170:Transformation, Genetic