title: Three-dimensional structure of proteins determined by molecular dynamics with interproton distance restraints: application to crambin.
abstract: Model calculations are performed to evaluate the utility of molecular dynamics with NMR interproton distance restraints for determining the three-dimensional structure of proteins. The system used for testing the method is the 1.5-A resolution crystal structure of crambin (a protein of 46 residues) from which a set of 240 approximate interproton distances of less than 4 A are derived. The convergence properties of the method are examined by using different dynamics protocols and starting from two initial structures; one is a completely extended beta-strand, and the other has residues 7-19 and 23-30 in the form of alpha-helices (as in the crystal structure) with the remaining residues in the form of extended beta-strands. In both cases global and local convergence to the correct final structure is achieved with rms atomic differences between the restrained dynamics structures and the crystal structure of 1.5-2.1 A for the backbone atoms and 2.1-2.8 A for all atoms; the averaged structure has backbone and all atom rms deviations of 1.3 and 1.9 A, respectively. Further, it is shown that a restrained dynamics structure with significantly larger deviations (i.e., 5.7 A for the backbone atoms) can be characterized as incorrect, independent of a knowledge of the crystal structure.
authors.forename: A T
authors.initials: AT
authors.lastname: Brünger
authors.forename: G M
authors.initials: GM
authors.lastname: Clore
authors.forename: A M
authors.initials: AM
authors.lastname: Gronenborn
authors.forename: M
authors.initials: M
authors.lastname: Karplus
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010940:Plant Proteins; D011487:Protein Conformation
chemical_list: D010940:Plant Proteins; C024934:crambin protein, Crambe abyssinica