title: Determination of spermatozoa concentration in the rat cauda epididymidis.
abstract: A method for estimating the concentration of spermatozoa in the rat cauda epididymidis is described. Treatment of a sperm suspension with 0.05% collagenase for 20-60 min or 0.025% trypsin for 1-2 min at 34-37 degrees C was found to result in consistently homogeneous sperm. Sperm concentration ranged from 152.5 to 230.0 x 10(7) spermatozoa/ml, with a mean of 187.7 +/- 5.6 (SEM) x 10(7) spermatozoa/ml.
authors.affiliation: Departamento de Estomatologia, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Brasil.
authors.forename: T L
authors.initials: TL
authors.lastname: Lamano-Carvalho
authors.forename: W G
authors.initials: WG
authors.lastname: Kempinas
mesh_terms: D000818:Animals; D004822:Epididymis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013076:Sperm Count / Q000379:methods*; D013079:Sperm Maturation