title: 'Lymphocyte-defined' antigens on corneal fibroblasts.
abstract: Human stromal (fibroblast) cultures were established from random, normal corneas obtained from the International Eye Bank in Baltimore. Cultures were assessed for the expression of Class I, Class II, monocyte/granulocyte and T-cell antigens before and after treatment with gamma or alpha interferon (IFN), interleukin 2, and 4 beta-phorbol 12 beta-myristate 13 alpha-acetate (PMA). Class I (B2 microglobulin) and monocyte/granulocyte (Leu M-1) antigens were evident before induction, natural killer cell (Leu 7) antigens were transiently expressed, and Class II (HLA-DR, Leu 10) antigens were not expressed until after induction with gamma IFN. PMA reduced the numbers of cells positive for Leu M-1 while alpha IFN and interleukin 2 had no effect on antigen expression. Kinetic studies of HLA-DR induction indicated that as little as 12 hours exposure to gamma IFN was sufficient to initiate induction and that 30% of the population was still expressing HLA-DR 7 days after removal of gamma IFN. The inducible expression of Class II antigens following lymphokine exposure may play a critical role in rejection of allografted corneas.
authors.forename: E
authors.initials: E
authors.lastname: Young
authors.forename: B A
authors.initials: BA
authors.lastname: McMillan
mesh_terms: D000328:Adult; D000941:Antigens / Q000032:analysis* / Q000145:classification; D003315:Cornea / Q000276:immunology*; D003319:Corneal Stroma / Q000166:cytology / Q000276:immunology*; D005347:Fibroblasts / Q000276:immunology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology*; D008875:Middle Aged
chemical_list: D000941:Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II