title: Isolation of bovine immunoglobulins and lactoferrin from whey proteins by gel filtration techniques.
abstract: Whey is a suitable source of immunoglobulins and lactoferrin to enrich infant formulas. Gel filtration on Sephacryl S-300 and on Fractogel TSK HW-55 was used to isolate immunoglobulins from colostral whey, acid whey, and Cheddar cheese whey. The SDS-PAGE and immunoelectrophoresis techniques indicated that the purity of the fractions from fractionation on Sephacryl S-300 was better than that by fractionation on TSK HW-55 column. Biological activity of fractions from the Sephacryl S-300 column as assessed by immunochemical analysis was 99, 83.3, and 92% for colostral, acid, and sweet wheys. The well-proven antimicrobial agent, lactoferrin, was isolated from sweet whey by heparin-attached Sepharose. Lactoferrin selectively adsorbed to the column was subsequently eluted with 5 mM Veronal-HCl containing .5 M NaCl, pH 7.4. Purity of the isolated protein was confirmed by SDS-PAGE and immunoelectrophoresis.
authors.affiliation: Department of Food Science, University of British Columbia, Vancouver, Canada.
authors.forename: S A
authors.initials: SA
authors.lastname: Al-Mashikhi
authors.forename: S
authors.initials: S
authors.lastname: Nakai
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007136:Immunoglobulins / Q000302:isolation & purification*; D007781:Lactoferrin / Q000302:isolation & purification*; D007782:Lactoglobulins / Q000302:isolation & purification*; D008894:Milk Proteins / Q000032:analysis*
chemical_list: D007136:Immunoglobulins; D007782:Lactoglobulins; D008894:Milk Proteins; D007781:Lactoferrin