title: Is the platelet phenolsulfotransferase involved in the sulfoconjugation of plasma catecholamines?
abstract: Catecholamines are predominantly present in the sulfoconjugated forms in human plasma. Phenolsulfotransferase (EC 2.8.2.1), which catalyses the sulfation of phenolic compounds, is widely distributed in human tissues. In blood, a phenolsulfotransferase, more specific for catecholamine sulfation is found exclusively in platelets. Free and sulfoconjugated catecholamines were measured in plasma and platelets of healthy volunteers and compared with those present in patients with uremia or pheochromocytoma to determine the ability of platelet phenolsulfotransferase to sulfurylate plasma catecholamines. In patients with pheochromocytoma, the rise in free and sulfoconjugated plasma catecholamines is accompanied by a simultaneous rise of these molecules in platelets. In uremia, where the level of plasma catecholamines is normal, the rise in the sulfoconjugates is not accompanied by a concomitant increase in either free or sulfoconjugated catecholamines in platelets. Platelet phenolsulfotransferase activity remains unchanged in pheochromocytoma and uremia. These data indicate that the platelet phenolsulfotransferase is involved in the sulfation of the catecholamines present in platelets, but its contribution, if any, to the high level of sulfoconjugated catecholamines found in plasma is negligible. This assertion is confirmed by our observations in thrombocytopenic patients. Indeed, despite the very low number of platelets and the absence of plasma phenolsulfotransferase activity, thrombocytopenic patients have normal plasma levels of free and sulfoconjugated catecholamines.
authors.forename: Y
authors.initials: Y
authors.lastname: Romain
authors.forename: S
authors.initials: S
authors.lastname: Demassieux
authors.forename: G
authors.initials: G
authors.lastname: D'Angelo
authors.forename: M
authors.initials: M
authors.lastname: Gyger
authors.forename: S
authors.initials: S
authors.lastname: Carrière
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000097:blood / Q000201:enzymology; D015239:Arylsulfotransferase; D001792:Blood Platelets / Q000201:enzymology*; D002395:Catecholamines / Q000097:blood*; D006801:Humans; D009638:Norepinephrine / Q000097:blood; D010673:Pheochromocytoma / Q000097:blood / Q000201:enzymology; D011187:Posture; D013431:Sulfates / Q000097:blood*; D013466:Sulfurtransferases / Q000097:blood*; D013921:Thrombocytopenia / Q000097:blood / Q000201:enzymology; D014511:Uremia / Q000097:blood / Q000201:enzymology
chemical_list: D002395:Catecholamines; D013431:Sulfates; D013466:Sulfurtransferases; D015239:Arylsulfotransferase; D009638:Norepinephrine