[Difference in the mechanism of self-assembly in vitro in tobacco mosaic virus and potato virus X].
The effects of 254 nm UV-irradiation of tobacco mosaic virus (TMV) and potato virus X (PVX) RNA preparations on the RNA ability to self-assembly in vitro with the viral coat proteins were studied. It was found that while TMV RNA ability to assemble with the homologous protein is rapidly inactivated by the UV-irradiation, PVX RNA ability to be encapsidated by the PVX coat protein is quite resistant to the irradiation. More than that, the irradiation of TMV RNA with the dose strongly inhibiting its assembly with the homologous protein, did not result in any significant inhibition of this RNA ability to be coated with the PVX protein. The results testify to the profound differences in the mechanisms of RNA-protein interactions in the processes of self-assembly in vitro of tobamoviruses and potexviruses.
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D036022:Capsid Proteins; D010942:Plant Viruses / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D011198:Solanum tuberosum / Q000382:microbiology; D013045:Species Specificity; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000235:genetics
D036022:Capsid Proteins; C009496:H protein, Tobacco mosaic virus; D014764:Viral Proteins
Razlichiia v mekhanizmakh samosborki in vitro virusa tabachnoĭ mozaiki i X-virusa kartofelia.