Purification and characterization of the alpha form of rat plasma kallikrein.
A four-step procedure was used to purify rat plasma kallikrein (RPK) with a relative molecular mass (Mr) of 87 kD (obtained both by gel filtration and SDS-PAGE), which indicates the purification of an alpha (intact) kallikrein, in contrast to previously described RPK preparations which had lower Mr (beta or degraded form). RPK is a neutral (pI 6.7) serine proteinase glycoprotein (15% carbohydrates) and contains (residues/mol): galactose (27), N-acetylglucosamine (24), mannose (13), glucose (13) and fucose (7). This purified alpha form of RPK has properties very similar to those of pure human and bovine kallikreins.
Kouyoumdjian
M
M
Departamento de Bioquímica, Escola Paulista de Medicina, São Paulo, Brasil.
Borges
D R
DR
Michelacci
Y M
YM
Guimarães
J A
JA
Sampaio
C A
CA
Prado
J L
JL
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007610:Kallikreins / Q000097:blood / Q000302:isolation & purification*; D008968:Molecular Conformation; D051381:Rats
D007610:Kallikreins