The human osteosarcoma cell line U-2 OS expresses a 3.8 kilobase mRNA which codes for the sequence of the PDGF-B chain.
A cDNA clone of about 2500 base pairs was prepared from the human osteosarcoma cell line U-2 OS by hybridizing with a v-sis probe. Sequence analysis showed that this cDNA contains the coding region for the PDGF-B chain. It is discussed that the mitogen secreted by these osteosarcoma cells contains the PDGF-B chain and is probably a homodimer of two B-chains.
Weich
H A
HA
Sebald
W
W
Schairer
H U
HU
Hoppe
J
J
D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis; D006801:Humans; D009857:Oncogenes; D012516:Osteosarcoma / Q000032:analysis*; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D004247:DNA