title: A DNA probe to distinguish the species Anopheles quadriannulatus from other species of the Anopheles gambiae complex.
abstract: DNA probes used previously to distinguish the species Anopheles gambiae sensu stricto, An.arabiensis, An.melas and An.merus were tested against An.quadriannulatus. Using these DNA probes, An.gambiae s.s. and An.quadriannulatus were indistinguishable. A genomic library was constructed for An.quadriannulatus. Differential screening of this genomic library with An.gambiae s.s. and An.quadriannulatus genomic DNAs identified a species-specific, repeated DNA sequence. When used as a hybridization probe, this DNA sequence clearly distinguished An.gambiae s.s. from An.quadriannulatus. A simplified protocol for the use of DNA probes is described which may be used to identify material squashed directly on to nitrocellulose filter paper.
authors.affiliation: Wolfson Unit of Molecular Genetics, Liverpool School of Tropical Medicine, UK.
authors.forename: K R
authors.initials: KR
authors.lastname: Gale
authors.forename: J M
authors.initials: JM
authors.lastname: Crampton
mesh_terms: D000818:Animals; D000852:Anopheles; D004247:DNA / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004247:DNA