Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
The expression of a hybrid gene formed by the promoter region of the Xenopus laevis vitellogenin gene B1 and the CAT coding region is regulated by estrogen when the gene is transfected into hormone-responsive MCF-7 cells. Furthermore, the 5' flanking region of the gene B1 alone can confer inducibility to heterologous promoters, although to a varying extent depending on the promoter used. Deletion mapping of he vitellogenin hormone-responsive sequences revealed that a 13 bp element 5'-AGTCACTGTGACC-3' at position -334 is essential for estrogen inducibility. We have shown previously that this 13 bp element is present upstream of several liver-specific estrogen-inducible genes.
Seiler-Tuyns
A
A
Walker
P
P
Martinez
E
E
Mérillat
A M
AM
Givel
F
F
Wahli
W
W
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D002872:Chromosome Deletion; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005796:Genes / Q000187:drug effects; D005914:Globins / Q000235:genetics; D006801:Humans; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000187:drug effects; D014819:Vitellogenins / Q000235:genetics; D014981:Xenopus
D014819:Vitellogenins; D004958:Estradiol; D005914:Globins; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase