Structural analysis of the 5' region of the chromosomal gene for hamster histidyl-tRNA synthetase.
The chromosomal gene (HRS) coding for hamster histidyl-tRNA synthetase, like many other housekeeping genes, lacks many of the features associated with promoters of RNA-polymerase-II-transcribed genes. HRS transcripts have multiple start points. Using RNase protection analysis, we also identified a 300-bp exon located only 36 bp away from the 5'-most start point of the HRS transcript. This exon hybridizes to a 3.5-kb transcript which transcribes from a different strand of DNA in the 5' region of the HRS gene. This divergent 3.5-kb transcript also has multiple transcription start points. The identity and function of this 3.5-kb transcript is not known.
Tsui
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FW
Mount Sinai Hospital Research Institute, Toronto, Ont., Canada.
Siminovitch
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L
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D006224:Cricetinae; D004274:DNA, Recombinant; D006643:Histidine-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012260:Ribonucleases; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012260:Ribonucleases; D000604:Amino Acyl-tRNA Synthetases; D006643:Histidine-tRNA Ligase