Modulatory action of purinergic drugs on high potassium-induced epileptiform bursting in rat hippocampal slices.
Increase of the potassium concentration up to 8 mM in the superfused solution of rat hippocampal slices leads to the development of an epileptiform bursting. The derivative agonist L-phenyl isopropyl adenosine (L-PIA) (0.05-0.5 microM) is able to block the potassium induced epileptiform activity. The adenosine antagonist caffeine (100 microM) reverts the antiepileptic effect of L-PIA. Our data show a modulatory action of the purinergic transmission in a model of experimental in vitro epilepsy, and point out about a control of endogenous adenosine in the development of focal epileptiform activity. The relationships between the purinergic influence on the release of neurotransmitters, and the convulsant-anticonvulsant effects of the drugs are discussed.
Sagratella
S
S
Laboratorio di Farmacologia, Istituto Superiore di Sanità, Roma, Italy.
Frank
C
C
Benedetti
M
M
Scotti de Carolis
A
A
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D004827:Epilepsy / Q000139:chemically induced / Q000503:physiopathology*; D006624:Hippocampus / Q000503:physiopathology*; D066298:In Vitro Techniques; D008274:Magnesium / Q000502:physiology; D008297:Male; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D011188:Potassium / Q000633:toxicity*; D011687:Purines / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D011687:Purines; D010660:Phenylisopropyladenosine; D002110:Caffeine; D008274:Magnesium; D011188:Potassium; D002118:Calcium