Activated H-ras transforms rat intestinal epithelial cells with expression of alpha-TGF.
We describe malignant transformation of cultured rat intestinal epithelial cells (IEC-18) by transfection with the activated human H-ras gene cloned from the EJ bladder carcinoma. Transformed cells showed a marked morphological change, expressed high levels of the transfected H-ras gene, were able to grow in agar and expressed antigenic markers identical with parental IEC-18 cells. When injected into syngeneic rats these cells formed rapidly growing tumors expressing the same intestinal-specific antigenic markers as the injected cells. Parallel to the high expression of H-ras mRNA in the transformants we document overexpression of rat alpha-TGF mRNA.
Buick
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Filmus
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Quaroni
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D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000503:physiopathology*; D004848:Epithelium / Q000276:immunology / Q000503:physiopathology; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000503:physiopathology*; D009857:Oncogenes; D010455:Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014162:Transfection; D015291:Transforming Growth Factors
D000951:Antigens, Neoplasm; D010455:Peptides; D012333:RNA, Messenger; D015291:Transforming Growth Factors