In vivo and in vitro modifications of the mitochondrial membrane induced by 4' Epi-adriamycin.
Mice received 4' Epi-adriamycin (4' Epi-ADM) i.p. in increasing doses. After 48 hours, hearts were removed and mitochondria were isolated. 4' Epi-ADM had no effect on the enzymatic activities of complexes I-III and complex IV of the mitochondrial respiratory chain; no modification of the mitochondrial membrane viscosity was observed and only a slight membrane lipid peroxidation was measured. On the contrary, in vitro studies showed a 4' Epi-ADM dependent enzymatic inactivation of complex I-III and IV, correlated with a mitochondrial membrane rigidification and an enhanced lipid peroxidation rate.
Praet
M
M
Laghmiche
M
M
Pollakis
G
G
Goormaghtigh
E
E
Ruysschaert
J M
JM
D000818:Animals; D004317:Doxorubicin / Q000633:toxicity*; D015251:Epirubicin; D005453:Fluorescence; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism; D008566:Membranes / Q000187:drug effects; D051379:Mice; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013329:Structure-Activity Relationship
D008054:Lipid Peroxides; D015251:Epirubicin; D004317:Doxorubicin