Prevention of lipid peroxidation by NAD(P)H in rat liver submitochondrial particles.
In rat liver submitochondrial particles both NADH and NADPH inhibit lipid peroxidation induced by cumene hydroperoxide. Concomitantly with the inhibition of lipid peroxidation, NADH and NADPH strongly stimulate the peroxidase activity of rat liver submitochondrial particles. Rotenone slightly prevents both the protective effect on malondialdehyde formation and peroxidase activity. The peroxidase activity of rat liver submitochondrial particles was attributed to the NAD(P)H-mediated reduction of mitochondrial cytochrome P-450 which can act upon hydroperoxides, by decomposing them to alcohols.
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Centro Studio Fisiologia Mitocondriale, CNR, Padova, Italy.
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Cavallini
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D000438:Alcohols / Q000096:biosynthesis; D000818:Animals; D001555:Benzene Derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology*; D009249:NADP / Q000494:pharmacology*; D010084:Oxidation-Reduction; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D013386:Succinates / Q000494:pharmacology
D000438:Alcohols; D001555:Benzene Derivatives; D008054:Lipid Peroxides; D013386:Succinates; D012402:Rotenone; D009243:NAD; D009249:NADP; C007164:cumene hydroperoxide