Regulated expression of cloned human fetal A gamma-globin genes introduced into murine erythroleukemia cells.
We have analysed the expression of cloned human fetal gamma-globin genes introduced into murine erythroleukemia cells by a protoplast fusion procedure. Both the wild-type a gamma-globin gene and a mutant derivative related to a phenotype of hereditary persistence of fetal hemoglobin were studied. In both cases the level of gamma-globin mRNA increased by a factor of approximately sevenfold when erythroid cell differentiation was induced by treatment with hexamethylenebisacetamide. Thus, the regulation of the expression of the cloned fetal A gamma-globin gene in murine erythroleukemia cells resembled that of cloned adult beta-globin genes.
Charnay
P
P
Henry
L
L
D000818:Animals; D002454:Cell Differentiation; D003001:Cloning, Molecular; D005326:Fetal Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D051379:Mice; D008957:Models, Genetic; D009154:Mutation
D005326:Fetal Proteins; D005914:Globins