Preparation and properties of antitumor monoclonal antibodies labeled with metallic radionuclides indium-111, gallium-67, and technetium-99m.
In this study, metallic radionuclides such as 111In, 67Ga, or 99mTc produced clear visualization in scintigraphic imaging of tumors; they have short half-lives and can easily be used in the radiolabeling of monoclonal antibodies by using bifunctional chelating agents. Under selected conditions, these radiolabeled antibodies were stable both in vitro and in vivo with no loss of the antigen-binding activity. Despite high background imaging of the liver and kidney, transplanted tumors in nude mice were clearly visualized with 111In-, 67Ga-, and 99mTc-labeled antibodies at 6, 24, or 48 hours after the injection. Although tissue distribution in the liver, kidney, and bone was different among radionuclides used for the labeling, all tumor to blood ratios were higher than those of radioiodinated monoclonal antibodies. These results provide a good basis for studies of the clinical usefulness of 111In-, 67Ga-, and 99mTc-labeled monoclonal antibodies for radioimmunoimaging.
Endo
K
K
Sakahara
H
H
Nakashima
T
T
Koizumi
M
M
Ohta
H
H
Kawamura
Y
Y
Kunimatsu
M
M
Furukawa
T
T
Ohmomo
Y
Y
Arano
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D005710:Gallium Radioisotopes; D006801:Humans; D007204:Indium; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000175:diagnosis*; D011868:Radioisotopes; D013667:Technetium
D000911:Antibodies, Monoclonal; D005710:Gallium Radioisotopes; D011868:Radioisotopes; D007204:Indium; D013667:Technetium