Enzyme activity dot blots: a rapid and convenient assay for acetyltransferase or protein kinase activity immobilized on nitrocellulose.
Methods are described for assaying (Tetrahymena) histone acetyltransferase activity and (Drosophila) casein kinase II activity by spotting extracts on nitrocellulose filters. The methods are quantitative over a wide range of enzyme concentrations and are almost as sensitive as liquid assays. Examples are presented for illustrating the use of these methods for enzyme purification, concentration, and desalting, as well as for electrophoretic blotting from agarose gels. A simple method for autoradiographic enhancement of nitrocellulose filters is also described.
Allis
C D
CD
Chicoine
L G
LG
Glover
C V
CV
White
E M
EM
Gorovsky
M A
MA
D000123:Acetyltransferases / Q000032:analysis*; D000818:Animals; D047388:Casein Kinases; D003101:Collodion; D004330:Drosophila / Q000201:enzymology; D004586:Electrophoresis; D004800:Enzymes, Immobilized; D051548:Histone Acetyltransferases; D011494:Protein Kinases / Q000032:analysis*; D029701:Saccharomyces cerevisiae Proteins; D013768:Tetrahymena / Q000201:enzymology
D004800:Enzymes, Immobilized; D029701:Saccharomyces cerevisiae Proteins; D003101:Collodion; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D011494:Protein Kinases; D047388:Casein Kinases