title: Rapid gas-chromatographic assay of bupivacaine in plasma.
abstract: A method for estimating bupivacaine concentration in human plasma by capillary gas-chromatography using solid injection and nitrogen-specific detection is described. Etidocaine, another anilidetype local anesthetic was used as internal standard and added to the sample before single-step extraction with diethylether. This method demonstrates high sensitivity (6 ng/ml plasma) and combines selectivity, rapidity, and simplicity. Results of this procedure correlate well with those obtained by an HPLC method.
authors.affiliation: Département de Pharmacologie, Faculté de Médecine, Centre Hospitalier Universitaire, Hôpital Laennec, Nantes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Le Normand
authors.forename: A
authors.initials: A
authors.lastname: Athouel
authors.forename: Y
authors.initials: Y
authors.lastname: Blanloeil
authors.forename: C
authors.initials: C
authors.lastname: De Villepoix
authors.forename: J C
authors.initials: JC
authors.lastname: Melchior
authors.forename: M F
authors.initials: MF
authors.lastname: Kergueris
authors.forename: M
authors.initials: M
authors.lastname: Bourin
authors.forename: C
authors.initials: C
authors.lastname: Larousse
mesh_terms: D002045:Bupivacaine / Q000097:blood* / Q000493:pharmacokinetics; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans
chemical_list: D002045:Bupivacaine