A rapid, simple method for isolation of viable microfilariae.
We describe a rapid, efficient method for purifying Loa loa microfilariae from blood that does not affect their longterm motility. It is based on the separation of microfilariae on a performed discontinuous Percoll gradient, followed by filtration on cellulose ester membranes. The parasite population, after isolation, is close to 100% pure, 100% viable and remains motile for at least 20 days. Recovery from original blood samples is typically around 90%. The method, because of its simplicity and minimal material requirements, could be used with up to 210 ml of blood. It also allows the isolation of Mansonella perstans microfilariae.
Van Hoegaerden
M
M
Ivanoff
B
B
D000818:Animals; D002500:Centrifugation, Isopycnic; D005368:Filariasis / Q000469:parasitology; D006801:Humans; D008117:Loa / Q000302:isolation & purification*; D008118:Loiasis / Q000469:parasitology; D008366:Mansonella / Q000302:isolation & purification*; D008368:Mansonelliasis / Q000469:parasitology