Effect of UVC and araC on L5178Y-R and L5178Y-S cells. Nucleoid sedimentation.
The effects of UVC radiation (lambda = 254 nm, 85 J/m2) and/or 1-beta-D-arabino-furanosylcytosine (araC, 2 x 10(-3) M, 2 h) on two mouse lymphoma cell lines, UVC-sensitive and X-ray resistant L5178Y-R and UVC-resistant and X-ray sensitive L5178Y-S, were investigated. AraC treatment inhibited the semiconservative DNA replication to 1.4% and 3.8% in L5178Y-R and L5178Y-S cells, respectively, and decreased the sedimentation distance of nucleoids from the cells of both lines. The shortening of sedimentation distances induced by UVC and araC treatment was 8.1 mm for L5178Y-R cells and 11.8 mm for L5178Y-S, and indicated a higher number of DNA breaks in L5178Y-S cells. Assuming that such breaks are the result of the inhibition of DNA repair replication by araC, we conclude that L5178Y-S cells have a greater number of repaired sites than L5178Y-R cells.
Walicka
M
M
Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warszawa, Poland.
Godlewska
E
E
Kleczkowska
H
H
D000818:Animals; D002461:Cell Line, Transformed; D003561:Cytarabine / Q000494:pharmacology*; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000528:radiation effects*; D051379:Mice; D014461:Ultracentrifugation; D014466:Ultraviolet Rays
D004273:DNA, Neoplasm; D003561:Cytarabine