[3H] (-)sulpiride binding in rat striatum, cortex and anterior pituitary: an improved assay.
The interaction of [3H] (-)sulpiride with D2 dopamine (DA) receptors in the striatum, anterior pituitary and medial-prefrontal cortex was studied in rats, using an improved [3H] (-)sulpiride radioreceptor binding technique. Incubation on ice and a fast filtration resulted in higher specific binding (approximately 85% of total binding) a lower affinity constant (about 3 nM) and higher Bmax than reported with previous procedures. Pharmacological interactions confirmed the high selectivity of [3H] (-)sulpiride for D2 DA receptors.
Stefanini
E
E
Department of Neuroscience, School of Medicine, University of Cagliari, Italy.
Ortu
A M
AM
Vernaleone
F
F
Gessa
G L
GL
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013469:Sulpiride / Q000378:metabolism*
D013469:Sulpiride