Increased radiation-induced transformation in C3H/10T1/2 cells after transfer of an exogenous c-myc gene.
C3H/10T1/2 cells were infected with a retroviral vector expressing a mouse c-myc oncogene and a drug-selection marker. The resulting cells, morphologically indistinguishable from C3H/10T1/2, displayed a greatly enhanced sensitivity to neoplastic transformation by ionizing radiation or by a chemical carcinogen. Constitutive expression of myc therefore appears to synergize with an initial carcinogenic event, providing a function analogous to a subsequent event that apparently is required for the neoplastic transformation of these cells. This cell system should prove useful in exploring early stages in radiation-induced transformation.
Sorrentino
V
V
Drozdoff
V
V
Zeitz
L
L
Fleissner
E
E
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005814:Genes, Viral; D005822:Genetic Vectors; D051379:Mice; D008809:Mice, Inbred C3H; D009381:Neoplasms, Radiation-Induced / Q000235:genetics / Q000473:pathology*; D011519:Proto-Oncogenes; D012190:Retroviridae / Q000235:genetics