title: Induction of differentiation in chronic myelocytic leukaemia cells by the phorbolester TPA.
abstract: The mononuclear peripheral blood cells from eight patients with chronic myelocytic leukaemia (CML) were incubated in cell suspension culture in the presence of the phorbolester 12-O-tetradecanoylphorbol 13-acetate (TPA). TPA caused the treated cells to adhere and to acquire morphological and functional features characteristic of macrophage-like cells. Using isoelectric focusing distinct changes in the isoenzyme profiles of carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase were detected in the TPA-exposed cells. Besides an increase in the number and staining intensity of isoenzymes of all enzymes, TPA triggered the new expression of a monocyte-specific esterase isoenzyme isoenzyme and of the tartrate-resistant acid phosphatase isoenzyme. The latter two isoenzymes represent further parameters of the monocyte/macrophage complex. The results indicate that immature leukaemic cells arrested along the granulocytic cell axis retain the ability to transform to macrophages.
authors.forename: H G
authors.initials: HG
authors.lastname: Drexler
authors.forename: N
authors.initials: N
authors.lastname: Bhoopalam
authors.forename: M
authors.initials: M
authors.lastname: Klein
authors.forename: G
authors.initials: G
authors.lastname: Gaedicke
authors.forename: J
authors.initials: J
authors.lastname: Minowada
mesh_terms: D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000473:pathology*; D008264:Macrophages / Q000201:enzymology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D013755:Tetradecanoylphorbol Acetate