Cytogenetic characterization of putative human myeloblastic leukemia cell lines (ML-1, -2, and -3): origin of the cells.
Cytogenetic studies were performed on ML cell lines (ML-1, -2, and -3), as well as on the leukemic cells of a patient from whom the ML cells were derived. The ML-1 cell line showed numerical and structural cytogenetic changes, i.e., -Y, 1p-, 6q-, 11q-, +12, +13q+, 14q-, and 17q-. The ML-2 cell line had two copies of the 13q+, whereas the ML-3 cells contained three clones, i.e., 47,X,-Y,1p-,6q-,11q-,+12,+13q+, 48,X,-Y,1p-,6q-,+6q-,11q-,+12,+13q+, and 49,X,-Y,1p-,6q-,+6q-, 11q-,+12,+13q+,+13q+. The neoplastic cells, when the patient was diagnosed as having T-cell malignant lymphoma (Stage IV), had the 11q- and 13q+. The leukemic cells in a subsequent acute myeloid leukemia phase of this patient contained structural (1p- and 6q-) and numerical (+12, -Y and +2D-group chromosomes: two 13q+) changes in addition to the 11q-. These findings suggest that the acute myeloid leukemic cells of this patient probably originated from the neoplastic cells of the preceding T-cell lymphoma, and that the chromosome changes originally seen in the lymphoma cells were preserved in the established ML cell lines, though the cells of these lines had myeloid characteristics.
Ohyashiki
K
K
Ohyashiki
J H
JH
Sandberg
A A
AA
D001853:Bone Marrow / Q000473:pathology; D002460:Cell Line; D002869:Chromosome Aberrations / Q000235:genetics; D002871:Chromosome Banding; D025063:Chromosome Disorders; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*