Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides.
Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-alpha detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.
Falloon
J
J
Malech
H
H
Milligan
G
G
Unson
C
C
Kahn
R
R
Goldsmith
P
P
Spiegel
A
A
D000936:Antigen-Antibody Complex; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007106:Immune Sera; D007700:Kinetics; D008565:Membrane Proteins / Q000097:blood* / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D009842:Oligopeptides / Q000097:blood*; D037342:Pertussis Toxin; D013379:Substrate Specificity; D015293:Transducin; D010566:Virulence Factors, Bordetella / Q000378:metabolism*
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D008565:Membrane Proteins; D009842:Oligopeptides; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D015293:Transducin