title: Effect of metronidazole on development of subgingival plaque and experimental periodontitis.
abstract: Active tissue destruction in experimental periodontitis has been positively correlated with subgingival spirochetes and total number of organisms. The present study was designed to inhibit spirochete populations and evaluate the effect upon periodontal destruction. Metronidazole was administered orally to four squirrel monkeys (100 mg/kg/bwt) for 17 days. After 3 days, marginal periodontitis was induced around bicuspids and molars by tying silk ligatures at the gingival margins. Subgingival plaque samples were taken baseline and 14 days after ligature placement. Dark-field microscopy quantitated motile forms, spirochetes, straight and curved rods, filament, cocci and fusiforms. Periodontal destruction was evaluated at 2 weeks by histometric analysis of connective tissue attachment, crestal alveolar bone and infiltrated connective tissue. Bacterial and histometric comparisons were made with experimental periodontitis data from four animals (control) which had not received metronidazole. Subgingival plaque prior to periodontitis induction was dominated by cocci, but fusiforms and straight rods were also present. Straight rods formed a greater proportion of the plaque which developed in metronidazole-receiving animals. The absence of spirochetes and motile rods contrasted with the control group where spirochetes were the predominant type. The total number of bacteria was also larger in the control animals. Histometric analysis showed that areas of infiltrated supracrestal connective tissue were similar in both groups. Loss of connective tissue attachment and alveolar bone was significantly less in experimental animals, and the latter values did not differ significantly from baseline dimensions. The results indicated that the subgingival bacterial populations which developed during metronidazole administration did not result in an experimental periodontitis.
authors.forename: A M
authors.initials: AM
authors.lastname: Polson
authors.forename: U E
authors.initials: UE
authors.lastname: Zappa
authors.forename: M A
authors.initials: MA
authors.lastname: Espeland
authors.forename: A D
authors.initials: AD
authors.lastname: Eisenberg
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification; D003238:Connective Tissue / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D003743:Dental Enamel / Q000473:pathology; D003773:Dental Plaque / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology*; D005881:Gingiva / Q000382:microbiology; D008795:Metronidazole / Q000494:pharmacology*; D010518:Periodontitis / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology*; D010519:Periodontium / Q000473:pathology; D012453:Saimiri
chemical_list: D008795:Metronidazole