title: Improved procedure for platelet freezing.
abstract: Platelets frozen in glycerol-glucose frequently aggregated on thawing or reconstitution. This has been eliminated by using a nonplasma diluent (pH 6.5) and a new freezing bag. The results were improved platelet 14C-serotonin uptake and readily demonstrable aggregation to ADP, epinephrine, collagen and thrombin. 14C-serotonin uptake was at maximum when platelets were frozen at thrombocrits of up to 15%. Hemostatic effectiveness was demonstrated in a severely thrombocytopenic patient with acute myelogenous leukemia in relapse, who was supported entirely with two autologous frozen platelet transfusions for the 13 days before bone marrow activity resumed.
authors.forename: G
authors.initials: G
authors.lastname: Dayian
authors.forename: H L
authors.initials: HL
authors.lastname: Harris
authors.forename: G D
authors.initials: GD
authors.lastname: Vlahides
authors.forename: J H
authors.initials: JH
authors.lastname: Pert
mesh_terms: D001692:Biological Transport; D001792:Blood Platelets / Q000502:physiology; D001793:Blood Preservation / Q000379:methods*; D005615:Freezing; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000628:therapy; D010974:Platelet Aggregation; D017713:Platelet Transfusion; D012701:Serotonin / Q000097:blood; D014182:Transplantation, Autologous
chemical_list: D012701:Serotonin