title: Enzymatic acylation of ether and ester lysophospholipids in rat liver microsomes.
abstract: The acylation of lysophospholipids by rat liver acyltransferases was studied. A comparison between ester and ether lysophospholipids as substrates revealed large differences in substrate properties. For instance, oleic acid from oleoyl-CoA and arachidonic acid from arachidonoyl-CoA were not incorporated into 1-O-octadecyl-sn-glycero-3-phosphocholine under experimental conditions that allowed an optimal transfer of oleic acid and arachidonic acid to 1-O-palmitoyl-sn-glycero-3-phosphocholine. However, we observed an acyl-CoA-independent transfer of arachidonic acid from 1-O-stearoyl-2-O-arachidonoyl-sn-glycero-3-phosphoinositol to 1-O-octadecyl-sn-glycero-3-phosphocholine.
authors.affiliation: Max-Planck-Institut für biophysikalische Chemie, Göttingen, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Neumüller
authors.forename: E A
authors.initials: EA
authors.lastname: Fleer
authors.forename: C
authors.initials: C
authors.lastname: Unger
authors.forename: H
authors.initials: H
authors.lastname: Eibl
mesh_terms: D000215:Acylation; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008246:Lysophospholipids / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D010742:Phospholipid Ethers / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
chemical_list: D000970:Antineoplastic Agents; D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010742:Phospholipid Ethers; C006065:We 201