Interferon inducing transformed cell surface glycoproteins: purification by Ia antigen affinity chromatography.
Ia antigens were previously shown to be B-cell receptors for transformed cell surface glycoproteins (TCSG). Ia antigen recognition of TCSG subsequently initiated interferon production. These findings have been exploited to highly purify TCSG from mouse L (H-2k) cells in one step by Iak antigen Sepharose affinity chromatography. These results point to the highly specific recognition of TCSG by Ia antigens and the B-cells on which they reside. The implications and applications of these studies are discussed.
Hughes
T K
TK
Smith
E M
EM
Blalock
J E
JE
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D000949:Histocompatibility Antigens Class II; D007369:Interferon Inducers / Q000302:isolation & purification*; D008214:Lymphocytes / Q000032:analysis; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D013154:Spleen
D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D007369:Interferon Inducers; D008565:Membrane Proteins