Phorbol ester induces c-sis gene transcription in stem cell line K-562.
The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) induced megakaryoblastic differentiation and c-sis expression in the human hematopoietic stem cell line K-562. This induction occurred at the transcriptional level, as determined by a nuclear runoff transcriptional assay, and was not a generalized effect of TPA, since the treatment of other hematopoietic cell lines and normal peripheral blood lymphocytes with TPA did not result in the appearance of c-sis mRNA.
Colamonici
O R
OR
Trepel
J B
JB
Vidal
C A
CA
Neckers
L M
LM
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D007951:Leukemia, Myeloid; D009857:Oncogenes / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate