title: Biologic properties of cell lines derived from canine mammary carcinomas.
abstract: Six canine mammary tumor cell lines (5 epithelial, 1 myoepithelial) were established from dogs with spontaneous mammary carcinomas. The cells were characterized for features of morphology and growth patterns on plastic, ultrastructure, cloning efficiency in soft agar, tumorigenicity in athymic nude mice, and hormonal receptor status. Biologic properties of the cell lines were distinct and preserved during long-term culture in vitro. All cell lines were clonogenic, 5 of 6 were tumorigenic, and 3 of 5 were receptor positive for estrogen and/or progesterone.
authors.forename: L G
authors.initials: LG
authors.lastname: Wolfe
authors.forename: B B
authors.initials: BB
authors.lastname: Smith
authors.forename: M A
authors.initials: MA
authors.lastname: Toivio-Kinnucan
authors.forename: E A
authors.initials: EA
authors.lastname: Sartin
authors.forename: R P
authors.initials: RP
authors.lastname: Kwapien
authors.forename: R A
authors.initials: RA
authors.lastname: Henderson
authors.forename: S
authors.initials: S
authors.lastname: Barnes
mesh_terms: D000818:Animals; D002460:Cell Line; D003470:Culture Media; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D005260:Female; D008321:Mammary Glands, Animal; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis
chemical_list: D003470:Culture Media; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone