title: Molecular cloning of mouse placental lactogen cDNA.
abstract: We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage lambda gt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDa polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-II antiserum. The mPL-II cDNA clones hybridize to a 1.0-kilobase placental-specific mRNA. This mRNA, found in the fetal portion of the placenta, appears as early as day 10 of gestation and increases to a maximal level by day 12. The mPL-II cDNA nucleotide sequence has been determined. This sequence contains an open reading frame encoding a polypeptide of 222 amino acids with the amino-terminal 31 amino acids forming the signal sequence for secretion. The predicted secreted protein has 51% amino acid homology with mouse prolactin.
authors.forename: L L
authors.initials: LL
authors.lastname: Jackson
authors.forename: P
authors.initials: P
authors.lastname: Colosi
authors.forename: F
authors.initials: F
authors.lastname: Talamantes
authors.forename: D I
authors.initials: DI
authors.lastname: Linzer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D010928:Placental Lactogen / Q000032:analysis / Q000235:genetics*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D004247:DNA; D010928:Placental Lactogen