title: Expression of adult fast pattern of acetylcholinesterase molecular forms by mouse satellite cells in culture.
abstract: The pattern of acetylcholinesterase (AChE) molecular forms, obtained by sucrose gradient sedimentation, was studied at different in vitro developmental stages of myogenic cells isolated from adult mouse skeletal muscle. Only the globular forms were present in rapidly dividing satellite cells during the first days in culture. After myotube formation, a pattern similar to that described in mammalian fast-twitch skeletal muscle was observed. This pattern did not change during the following period in culture (up to 1 month) nor could it be modified by co-culturing with spinal cord motoneurons or by addition of brain-derived extracts. The internal-external localization of AChE molecular forms has been determined by the use of echothiophate iodide, a membrane-impermeant irreversible inhibitor of AChE. Echothiophate-treated cultures showed about 40% of both asymmetric and globular forms localized on the sarcolemma, with their active sites oriented outward. Analysis of culture medium from untreated cultures revealed the presence of both asymmetric and globular forms. When the same analysis was repeated on cultures of myoblasts derived from 16-day-old mouse embryos, the pattern of AChE forms was different. The myotubes derived from these cells exhibit a very small proportion of asymmetric form, which was not released into the medium. This pattern was not further modified during the following days of culture, nor by co-cultures with spinal cord motoneurons or by incubations with brain-derived extracts. Thus, the myotubes derived from myoblasts express in culture a clear phenotypic difference when compared to the corresponding myotubes from satellite cells, supporting the view that these two myogenic cells are endowed with different developmental programs.
authors.affiliation: Istituto di Istologia ed Embriologia Generale, Università di Roma, La Sapienza, Italy.
authors.forename: M I
authors.initials: MI
authors.lastname: Senni
authors.forename: F
authors.initials: F
authors.lastname: Castrignano
authors.forename: G
authors.initials: G
authors.lastname: Poiana
authors.forename: G
authors.initials: G
authors.lastname: Cossu
authors.forename: G
authors.initials: G
authors.lastname: Scarsella
authors.forename: S
authors.initials: S
authors.lastname: Biagioni
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation; D051379:Mice; D008968:Molecular Conformation; D009132:Muscles / Q000166:cytology* / Q000201:enzymology / Q000294:innervation; D013116:Spinal Cord / Q000166:cytology; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D000110:Acetylcholinesterase