Biphenotypic Philadelphia positive, monosomy seven acute leukaemia: ultrastructural immunocytochemical study.
A case of Ph1 positive acute leukaemia is presented in which an additional chromosome change, monosomy 7 was found. There was no clinical evidence of a pre-existing chronic myeloid leukaemia. Cytochemistry and immunology showed a predominant HLA-DR+, TdT+, cALL- phenotype, with a small percentage of HLA-DR+, Leu-Ml+ and cALL- cells. The true biphenotypic nature of this case was clearly shown by transmission electron microscopy using the immunogold method combined with myeloperoxidase (MPO). Two distinct phenotypes, lymphoid (cALL+, MPO-) and myeloid (LeuMl+, MPO+) were identified with this technique. An immuno-scanning electron microscope technique was also used to study this case, which demonstrated the presence of two different surface morphologies.
Soligo
D
D
Polli
N
N
Cattoretti
G
G
Cantu'-Rajnoldi
A
A
Romitti
L
L
De Harven
E
E
Lambertenghi-Deliliers
G
G
D001853:Bone Marrow / Q000648:ultrastructure; D002872:Chromosome Deletion; D002906:Chromosomes, Human, 6-12 and X; D005260:Female; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000648:ultrastructure*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009006:Monosomy; D010641:Phenotype; D010677:Philadelphia Chromosome