Changes in intracellular K+ and Na+ ion concentrations during cell growth and differentiation.
We compared intracellular K+ and Na+ ion concentrations during cell growth and differentiation of a mouse myeloid leukemia M1 cell line. Cells undergoing mitosis had higher K+ concentrations than quiescent cells. Treatment with a K+ channel blocker and furosemide enhanced cell growth and produced a slight increase in the intracellular K+ concentration. Treatment with reagents that reduced the intracellular K+ concentration stopped cell growth. Induction of differentiation in this cell line produced a decrease in the K+ concentration, which always was accompanied by an increase in the Na+ concentration. Treatment with ouabain, which decreased the intracellular K+ concentration, did not, however, induce differentiation in the M1 cell line. The data suggest that cell growth and differentiation in the M1 cells are accompanied by changes in the intracellular K+ and Na+ concentrations but that the changes in the contents of these monovalent cations do not necessarily induce differentiation in this cell line.
Takagi
K
K
Okabe
Y
Y
Yoshimura
K
K
Ichikawa
Y
Y
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D005665:Furosemide / Q000494:pharmacology; D007951:Leukemia, Myeloid / Q000473:pathology; D051379:Mice; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011743:Pyrimidines / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*
D011743:Pyrimidines; C034821:4-aminopyrimidine; D010042:Ouabain; D005665:Furosemide; D012964:Sodium; D011188:Potassium