Molecular characteristics of chronic myelogenous leukemia in blast crisis.
We have studied the expression of c-abl and c-myc in leukemic cells of patients in all clinical phases of chronic myelogenous leukemia. We demonstrate that an aberrant 8-Kb c-abl related transcript is present in the RNA of the leukemic cell from all patients with Ph+ CML and that the loss of both normal chromosome #9 is associated with the loss of the normal c-abl related transcripts. This represents direct evidence that the normal c-abl related transcripts derive from the normal c-abl gene locus on the normal chromosome #9, while the aberrant c-abl related transcript in Ph+ CML derives from the hybrid bcr-abl gene formed as a result of the t(9;22). We further demonstrate that trisomy 8 in some instances is associated with enhanced expression of the c-myc oncogene.
Blick
M
M
Romero
P
P
Talpaz
M
M
Kurzrock
R
R
Shtalrid
M
M
Andersson
B
B
Trujillo
J
J
Beran
M
M
Gutterman
J
J
D001752:Blast Crisis / Q000235:genetics*; D002460:Cell Line; D002869:Chromosome Aberrations; D005786:Gene Expression Regulation; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes