title: Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
abstract: The expression of a hybrid gene formed by the promoter region of the Xenopus laevis vitellogenin gene B1 and the CAT coding region is regulated by estrogen when the gene is transfected into hormone-responsive MCF-7 cells. Furthermore, the 5' flanking region of the gene B1 alone can confer inducibility to heterologous promoters, although to a varying extent depending on the promoter used. Deletion mapping of he vitellogenin hormone-responsive sequences revealed that a 13 bp element 5'-AGTCACTGTGACC-3' at position -334 is essential for estrogen inducibility. We have shown previously that this 13 bp element is present upstream of several liver-specific estrogen-inducible genes.
authors.forename: A
authors.initials: A
authors.lastname: Seiler-Tuyns
authors.forename: P
authors.initials: P
authors.lastname: Walker
authors.forename: E
authors.initials: E
authors.lastname: Martinez
authors.forename: A M
authors.initials: AM
authors.lastname: Mérillat
authors.forename: F
authors.initials: F
authors.lastname: Givel
authors.forename: W
authors.initials: W
authors.lastname: Wahli
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D002872:Chromosome Deletion; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005796:Genes / Q000187:drug effects; D005914:Globins / Q000235:genetics; D006801:Humans; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000187:drug effects; D014819:Vitellogenins / Q000235:genetics; D014981:Xenopus
chemical_list: D014819:Vitellogenins; D004958:Estradiol; D005914:Globins; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase