Transforming growth factors from human colonic carcinoma cells induced molecular alterations in untransformed mouse AKR embryo fibroblasts.
Over 700 polypeptide spots could be detected by two-dimensional electrophoretic analyses of membranes prepared from the murine AKR fibroblastic cells. Out of this abundance of polypeptides, only 9 polypeptide spots were found to be differentially expressed between the untransformed AKR-2B cells and their methylcholanthrene-transformed counterparts, the AKR-MCA cells. Treatment of the untransformed AKR-2B cells with transforming growth factors, prepared from the serum-free conditioned medium of HCT 116 MOSER human colonic carcinoma cells, induced the altered expression of 6 of these polypeptides which paralleled the electrophoretic profile of their permanently transformed counterparts, the AKR-MCA cells.
Chakrabarty
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Brattain
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D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003110:Colonic Neoplasms; D005347:Fibroblasts / Q000166:cytology; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008806:Mice, Inbred AKR; D009363:Neoplasm Proteins / Q000032:analysis; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D015291:Transforming Growth Factors
D008565:Membrane Proteins; D009363:Neoplasm Proteins; D010455:Peptides; D015291:Transforming Growth Factors