Relationship between the c-myb locus and the 6q-chromosomal aberration in leukemias and lymphomas.
Deletions of the long arm of chromosome 6 (6q-) are frequently found in hematopoietic neoplasms, including acute lymphoblastic leukemias, non-Hodgkin lymphomas and (less frequently) myeloid leukemias. The c-myb proto-oncogene has been mapped to region 6q21-24, which suggests that it could be involved in the 6q- aberrations. By means of in situ chromosomal hybridization on cells from six hematopoietic malignancies, it was demonstrated that the c-myb locus is not deleted, but is retained on band q22, which is consistently bordered by the chromosomal breakpoints in both interstitial and terminal 6q- deletions. The deletion breakpoints were located at some distance from the myb locus since no rearrangement of c-myb sequences was found. In one case, however, amplification of the entire c-myb locus was detectable. Furthermore, in all cases tested that carry 6q- deletions, myb messenger RNA levels were significantly higher than in normal cells or in malignant cells matched for lineage and stage of differentiation but lacking the 6q- marker. These results indicate that 6q- deletions are accompanied by structural and functional alterations of the c-myb locus and that these alterations may be involved in the pathogenesis of leukemias and lymphomas.
Barletta
C
C
Pelicci
P G
PG
Kenyon
L C
LC
Smith
S D
SD
Dalla-Favera
R
R
D002872:Chromosome Deletion; D002896:Chromosomes, Human, Pair 6; D004247:DNA / Q000235:genetics; D005784:Gene Amplification; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D007945:Leukemia, Lymphoid / Q000235:genetics; D007951:Leukemia, Myeloid / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000235:genetics
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D004247:DNA