Sensitivity and specificity of commercial ELISA kits for screening anti-LAV/HTLV III.
Two panels consisting of 236 and 258 anti-LAV/HTLV III positive and 64 and 50 negative sera, respectively (determined in Western blots), were tested with 8 different, commercially available ELISA test kits for detecting LAV/HTLV III antibodies (Abbott, Dupont, Electro-Nucleonics, Litton, Organon, Pasteur, Sorin, Wellcome). Positive sera were selected for levels of antibodies ranging from average to very high, and the negative sera included both negative sera and sera with antibodies to cellular components such as HLA antigens or nuclear membranes. In examination of the 2 panels, the sensitivity of the tests ranged from 97.2 to 100%, and the specificity from 70.0 to 100%. No test was ideal.
Gürtler
L G
LG
Eberle
J
J
Lorbeer
B
B
Deinhardt
F
F
D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic