title: Phospholipid/Ca2+-dependent protein kinase activity in human parathyroid adenoma.
abstract: We have examined the activities of phospholipid/Ca2+-dependent and cyclic AMP-dependent protein kinases of the parathyroid adenomas and the atrophic glands which were resected from three patients with primary hyperparathyroidism. Phospholipid/Ca2+-dependent protein kinase activity of atrophic parathyroid gland was exclusively present in cytosol fraction (90.7 +/- 12.3%). On the other hand, phospholipid/Ca2+-dependent protein kinase activity of parathyroid adenomas was 66.9 +/- 6.4% in cytosol and 33.1 +/- 6.4% in membrane fraction, suggesting a translocation of the enzyme from the cytosol to the membranes. Cyclic AMP-dependent protein kinase activity appeared to be higher in parathyroid adenoma than in atrophic parathyroid gland in both cytosol and membrane fractions.
authors.affiliation: Third Department of Internal Medicine, Gifu University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishizuka
authors.forename: K
authors.initials: K
authors.lastname: Kajita
authors.forename: K
authors.initials: K
authors.lastname: Kamikubo
authors.forename: T
authors.initials: T
authors.lastname: Komaki
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Nagao
authors.forename: Y
authors.initials: Y
authors.lastname: Nozawa
mesh_terms: D000236:Adenoma / Q000201:enzymology* / Q000503:physiopathology; D000328:Adult; D000368:Aged; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D005260:Female; D006801:Humans; D006961:Hyperparathyroidism / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010280:Parathyroid Glands / Q000378:metabolism; D010281:Parathyroid Hormone / Q000378:metabolism; D010282:Parathyroid Neoplasms / Q000201:enzymology* / Q000503:physiopathology; D011493:Protein Kinase C / Q000378:metabolism*
chemical_list: D010281:Parathyroid Hormone; D011493:Protein Kinase C