title: Elevation of adenylate cyclase activity during leukemic cell differentiation.
abstract: Exposure of the various human myeloid leukemic cell lines (HL60 and RDFD) to various compounds results in marked differentiation of the cells. This differentiation is associated with a marked increase in both basal and NaF-stimulated adenylate cyclase (AC) activity. The increase in AC activity occurs regardless of the differentiation inducer one has utilized (retinoic acid (RA), dimethyl formamide (DMF), hypoxanthine (HPX) or actinomycin D (actD) and is correlated with this process, as a variant of the HL60 cell (HL60-Blast) that does not differentiate upon exposure to the various inducers does not demonstrate this increase in AC activity. In addition, the differentiation process is associated with a rapid increase in intracellular cAMP within hours of adding the inducer, followed by a gradual decrease.
authors.forename: J
authors.initials: J
authors.lastname: Fontana
authors.forename: G
authors.initials: G
authors.lastname: Miksis
authors.forename: J
authors.initials: J
authors.lastname: Durham
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology
chemical_list: D012969:Sodium Fluoride; D000262:Adenylyl Cyclases