Synergy of monocytes and lymphocytes from idiopathic minimal lesion nephrotic patients in relapse in the production of the supernatant factor that increases rat glomerular basement membrane sulfate uptake.
We have previously shown a significant increase in 35sulfate uptake in rat glomerular basement membrane (GBM) when glomeruli were cocultured with peripheral blood mononuclear cells (PBMC) from idiopathic minimal lesion nephrotic syndrome (IMLNS) patients in relapse or with the supernatants of the same PBMC cultures. The purpose of this study was to determine the cell source of the supernatant factor. Rat glomeruli were cocultured with PBMC, with monocytes or with lymphocytes from 11 patients with IMLNS in relapse. Monocytes and lymphocytes were separated using adherence to plastic technique. Rat glomeruli cultured without PBMC served as controls. There was a significant increase in 35sulfate uptake in the GBM when glomeruli were cocultured with PBMC (geometric mean [GM]: 794 cpm/mg dry glomerular weight) as compared to glomeruli cultured with monocytes (GM: 316) (p less than 0.0005), glomeruli cultured with lymphocytes (GM: 309) (p less than 0.05), and glomeruli cultured alone (GM: 302) (p less than 0.00005). No significant differences in 35sulfate uptake were seen between monocytes, lymphocytes and glomeruli alone. These data showed that monocytes and lymphocytes are needed for the production of the supernatant factor that increases rat GBM 35SO4 uptake. Based on these and previous data (Concanavalin A stimulation of IMLNS PBMC results in an increased GBM uptake of 35sulfate, Pediatric Research 20: 321, 1986), we postulate that monocytes could trigger or amplify the production of the supernatant factor by lymphocytes.
Garin
E H
EH
Department of Pediatrics, Medical University of South Carolina, Charleston.
Boggs
K P
KP
D000293:Adolescent; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007678:Kidney Glomerulus / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D009402:Nephrosis, Lipoid / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012008:Recurrence; D013431:Sulfates / Q000378:metabolism*
D013431:Sulfates