Introduction of genes into preimplantation mouse embryos by use of a defective recombinant retrovirus.
Two-cell and four-cell preimplantation mouse embryos were cocultured in vitro with fibroblasts producing the recombinant retrovirus M-MuLV-neo. No wildtype helper virus was detected in these cultures. Of the embryos that survived the in vitro cultivation and the reimplantation into foster mothers, 2 of 15 that were tested contained the proviral genome. The provirus integrated as a single copy at a unique site. We estimate that approximately equal to 20% of the cells in each of the two transgenic fetuses contained the provirus.
Rubenstein
J L
JL
Nicolas
J F
JF
Jacob
F
F
D000818:Animals; D002970:Cleavage Stage, Ovum; D003673:Defective Viruses / Q000235:genetics*; D004351:Drug Resistance; D047108:Embryonic Development; D005260:Female; D005798:Genes, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D051379:Mice / Q000196:embryology*; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009030:Mosaicism; D009355:Neomycin / Q000494:pharmacology; D011247:Pregnancy; D014162:Transfection
D009355:Neomycin