Direct examination of the clonality of carcinogen-induced colonic epithelial dysplasia in chimeric mice.
The clonal composition of neoplastic foci was examined in histological sections of the colonic epithelium of azoxymethane (CAS: 25843-45-2)-treated CBA/Ca----C57BL/6J mouse aggregation chimeras, with the use of H-2 antigens as markers of cellular genotype. Each of 55 early neoplastic foci occurring at a mosaic patch boundary was composed of cells of a single genotype. Our results provide direct evidence that these foci arise from single crypts. In contrast, the epithelium of 5 of 17 larger adenomas was of mixed genotype: In 3, one genotype was represented only by a rim of cytologically normal epithelium derived from adjacent crypts, but in the other 2 the epithelium of both genotypes was dysplastic. One of these was probably a "collision" tumor arising from adjacent but independent foci; in the other, the minority component may have been derived from entrapped non-neoplastic crypts.
Ponder
B A
BA
Wilkinson
M M
MM
D000818:Animals; D002678:Chimera; D002999:Clone Cells / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D005838:Genotype; D006183:H-2 Antigens / Q000032:analysis / Q000235:genetics; D006651:Histocytochemistry; D051379:Mice; D008808:Mice, Inbred CBA
D006183:H-2 Antigens