Polysialic acid units are spatially and temporally expressed in developing postnatal rat kidney.
We report the presence of alpha(2----8)-linked polysialic acid as detected with a monoclonal antibody outside the nervous tissue in the postnatal developing rat kidney. By immunoblot analysis, the reactivity was confined to a broad band of apparent molecular mass 200-250 kDa. By immunohistochemistry, the polysialic acid units were found throughout the ureteric bud-derived collecting duct system. In developing nephrons derived from the metanephrogenic mesenchyme, polysialic acid units were only regionally and transiently expressed. Furthermore, the expression of polysialic acid units was developmentally regulated, as evidenced by their gradual disappearance concomitant with postnatal kidney maturation. These findings suggest a possible role for polysialic acid units in cell-cell contact-mediated renal differentiation processes.
Roth
J
J
Taatjes
D J
DJ
Bitter-Suermann
D
D
Finne
J
J
D000375:Aging; D000818:Animals; D006651:Histocytochemistry; D007668:Kidney / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D008970:Molecular Weight; D011134:Polysaccharides / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000096:biosynthesis* / Q000302:isolation & purification
D011134:Polysaccharides; D012794:Sialic Acids; C021319:polysialic acid