title: Substrate requirements for the isolation and purification of thymic epithelial cells.
abstract: Cultures of thymic epithelial cells were initiated and maintained in vitro using surfaces coated with different substrates. The substrates we compared were plastic commercially treated for tissue culture, plastic coated with gelatin, and plastic coated with Cell-TakTM, a new commercially available cell and tissue adhesive. Cell-TakTM-treated plates provided the best environment for isolation and purification of this cell type as judged by lack of fibroblast contamination, growth rate, and cell morphology.
authors.affiliation: Department of Pathology, University of Connecticut Health Center, Farmington 06032.
authors.forename: T
authors.initials: T
authors.lastname: Munoz-Blay
authors.forename: C V
authors.initials: CV
authors.lastname: Benedict
authors.forename: P T
authors.initials: PT
authors.lastname: Picciano
authors.forename: S
authors.initials: S
authors.lastname: Cohen
mesh_terms: D000818:Animals; D003094:Collagen; D004847:Epithelial Cells; D005347:Fibroblasts; D051379:Mice; D008809:Mice, Inbred C3H; D010969:Plastics; D013950:Thymus Gland / Q000166:cytology*; D014014:Tissue Adhesives
chemical_list: D010969:Plastics; D014014:Tissue Adhesives; D003094:Collagen