A model for the spatio-temporal organization of DNA replication in mammalian cells.
The spatio-temporal organization of chromosomal DNA replication was analyzed using a model based on a "DNA unit" (or decondensation unit) hypothesis. The model is an extension of the fork movement theory of Huberman & Riggs (1968) and can account for a partially deterministic and partially stochastic order of DNA replication in chromosomes. It presumes that each chromosome is composed of DNA units that are arranged in sequence and that are replicated in parallel. A deterministic wave of chromatin decondensation propagates along the DNA unit continuously and progressively providing a field for the random activation of replication origin. Assignment of replication times to DNA compartments by a Monte Carlo method was programmed based on the model and the program was used to stimulate DNA synthesis rate curves that can be measured by the method of Dolbeare et al. (1983, 1985). The shape of the curve is shown to constrain possible parameter values of the model, which include the rate of fork movement, the fraction of chromatin that is decondensed at the start of S-phase, the initial number of origins activated, the rate at which new origins are activated, etc. The chromosomal organization that controls the molecular level of DNA replication is briefly reviewed and its relevance to the model is also discussed.
Takahashi
M
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Department of Pathology, Yamaguchi University School of Medicine, Ube, Japan.
D000465:Algorithms; D000818:Animals; D002843:Chromatin / Q000032:analysis; D002871:Chromosome Banding; D002875:Chromosomes / Q000032:analysis; D003198:Computer Simulation; D004261:DNA Replication; D006801:Humans; D007399:Interphase; D008322:Mammals; D008433:Mathematics; D008957:Models, Genetic; D009010:Monte Carlo Method; D013997:Time Factors
D002843:Chromatin