Effects of prostaglandins E-2 and F-2 alpha on the metabolism of [U-14C]glucose by mouse morulae-early blastocysts in vitro.
During 5-h culture in the presence of radioactive glucose, PGE-2 (10 micrograms/ml) significantly inhibited incorporation of glucose into the acid-soluble glycogen pool. PGE-2 at 1 and 10 micrograms/ml and PGF-2 alpha at 1 microgram but not 10 micrograms/ml stimulated incorporation of glucose into non-glycogen macromolecules during culture. However, the utilization of acid-soluble glycogen and other biochemical pools was not affected by the presence of PGs in the medium during 24-h chase culture of pulse-labelled embryos. Carbon dioxide production was significantly suppressed in the presence of PGs but accumulation of lactate was not affected. The results indicate that PGE-2 and PGF-2 alpha, in physiological concentrations, directly influence the metabolism of glucose by preimplantation embryos.
Khurana
N K
NK
Wales
R G
RG
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D002970:Cleavage Stage, Ovum / Q000378:metabolism*; D015237:Dinoprost; D015232:Dinoprostone; D005260:Female; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D009028:Morula / Q000187:drug effects / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*
D011458:Prostaglandins E; D011460:Prostaglandins F; D015237:Dinoprost; D005947:Glucose; D015232:Dinoprostone