title: Human cytochrome P-450 4 mRNA and gene: part of a multigene family that contains Alu sequences in its mRNA.
abstract: Several overlapping lambda gt11 cDNA clones have been sequenced and shown to encode for the full-length human cytochrome P-450 4. The structure and location of the exons and flanking intron regions were also identified from a lambda EMBL-3 human genomic clone that encodes the full-length human P-450 4 gene. The human P-450 4 mRNA is flanked by 62 base pairs of 5'- and 1508 base pairs of 3'-noncoding sequence, with 1548 bases that encode a protein of 516 amino acids (Mr, 58,376). The predicted amino acid sequence of human P-450 4 is 69% and 70% homologous to its equivalent in mouse and rat, respectively, 75% homologous to rabbit P-450 4, and 68% homologous to human P1-450. The 7.6-kilobase gene encodes 3118 nucleotides of exon sequence that is separated by six introns into seven exons. Exon 7, which is 1802 nucleotides, contains three inverse/complement Alu sequences that are organized in tandem. Comparison of the genomic DNA sequence of the human P-450 4 gene with the human P1-450 and related genes in rat and mouse and the identification of the amino acid residues and triplet codon at each exon-intron junction show that the location of each intron in the human P-450 4 gene is conserved within this gene family. Although the length and homology of the introns within a related gene family may not be conserved, the location of intronic sequences may be an important determinant in the identification of related P-450 genes.
authors.forename: L C
authors.initials: LC
authors.lastname: Quattrochi
authors.forename: U R
authors.initials: UR
authors.lastname: Pendurthi
authors.forename: S T
authors.initials: ST
authors.lastname: Okino
authors.forename: C
authors.initials: C
authors.lastname: Potenza
authors.forename: R H
authors.initials: RH
authors.lastname: Tukey
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000032:analysis; D005796:Genes; D006801:Humans; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D012333:RNA, Messenger; D004247:DNA; D003577:Cytochrome P-450 Enzyme System