Nitrosamine carcinogen activation pathway determined by quantum chemical methods.
Multiple reactions are thought to be involved in transforming dialkynitrosamines to active carcinogens. The first proposed step is enzymatic alpha-hydroxylation by the active oxygen species of cytochrome P-450, followed by nonenzymatic N-dealkylation and formation of diazohydroxides (RNNOH). The latter transformation can reasonably occur by a two-step mechanism via tautomerization of a monoalkylnitrosamine intermediate or directly from the alpha-hydroxylated species in one step. Both of these pathways in the transformation of hydroxymethylnitrosamine to diazohydroxide and formaldehyde were examined by the semiempirical molecular orbital method MNDO (modified neglect of diatomic differential overlap) and the ab initio method using STO-3G and 3-21G basis sets. Complete geometry optimizations of all reactants, intermediates, transition states, and products were performed. MNDO was also used to compare the similar transformation of the dimethyl analog. Both methods show that in the gas phase a concerted pathway involving a six-membered ring transition-state pathway is kinetically favored over a two-step pathway involving N-demethylation followed by tautomerization via two four-membered ring transition states. This reaction appears to be a viable one to formation of an ultimate carcinogen by parent dialkylnitrosamines in the hydrophobic substrate binding site of cytochrome P-450.
Poulsen
M
M
Life Sciences Division, SRI International, Menlo Park, California.
Spangler
D
D
Loew
G H
GH
D001711:Biotransformation; D002273:Carcinogens; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D009602:Nitrosamines; D011789:Quantum Theory; D013329:Structure-Activity Relationship
D002273:Carcinogens; D009602:Nitrosamines; D003577:Cytochrome P-450 Enzyme System