Polymorphisms of four hepatic cytochromes P-450 in twenty-eight inbred strains of rat.
Two-dimensional gel electrophoresis of hepatic microsomes from phenobarbital-treated animals was used to analyze electrophoretic/regulatory polymorphisms for cytochromes P-450b, P-450e, P-450g, and P-450h in 28 inbred strains of rat. Previous studies with outbred rats revealed the existence of four electrophoretic variants for P-450b, two for P-450e, and three for P-450h as well as two regulatory alleles for P-450g. With the exception of one allozymic form of P-450h, all of these alleles as well as a novel (null) allele for P-450e were found to be homozygous in at least two of the inbred strains tested. Eight phenotypes for combinations of these four cytochromes P-450 were observed. Inbred strains were identified that can be used in studies on the structure/function of unique cytochrome P-450-allozymes and in genetic crosses to map the four distinct cytochrome P-450 genes.
Rampersaud
A
A
Department of Chemistry, Kent State University, Ohio 44242.
Walz
F G
FG
D000483:Alleles; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004586:Electrophoresis; D005260:Female; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010641:Phenotype; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains
D003577:Cytochrome P-450 Enzyme System