Continuing rearrangement of immunoglobulin and T-cell receptor genes in a Ha-ras-transformed lymphoid progenitor cell line.
The arrangement of immunoglobulin genes has been examined in a series of lymphoid cell lines transformed with the Harvey murine sarcoma virus. One cell line, HAFTL-1, expresses antigenic markers characteristic of B-lymphoid cells and undergoes frequent rearrangement at the JH locus (where J = joining and H = heavy chain) during propagation in culture. By molecular cloning and nucleotide sequence determination, these rearrangements were found to represent the earliest postulated step in heavy chain gene assembly: the joining of a diversity (D) segment to a JH segment. The HAFTL-1 cell line also undergoes infrequent D beta-to-J beta joining at the T-cell receptor beta locus in culture. The observations presented here suggest that the HAFTL-1 cell line represents the early stage of B-cell differentiation at which immunoglobulin gene rearrangement is initiated.
Alessandrini
A
A
Pierce
J H
JH
Baltimore
D
D
Desiderio
S V
SV
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D005796:Genes; D006251:Harvey murine sarcoma virus / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D009857:Oncogenes; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell; D004247:DNA