Electron microscopic and immunohistochemical observations of differentiation of human myeloid leukemia line, PL-21.
A new human myeloid leukemia cell line, PL-21, consisting of promyelocytes, was microscopically and immunohistochemically studied for their maturation induced by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and retinoic acid (RA). More than 80% of PL-21 cells cultured for 4 days with 10 ng/ml of TPA became macrophage-like cells with functional and histochemical properties consistent with monocytes/macrophages. The cells adhered to the plastic flask, developed phagocytic activity and macrophage-specific intracytoplasmic alpha subunit of S-100 protein, and had reduced myeloid-specific cytochemical markers. In contrast, RA-treated PL-21 cells displayed mature neutrophil-like morphology after 7 days of exposure. Most of the cells had reduced nitro blue tetrazolium and acquired phagocytic activity with persistence of myeloid-specific cytochemical markers such as peroxidase, naphthol-AS-D chloroacetate esterase, and Sudan black B. These results indicate that the PL-21 cell line ca be induced to mature into two directions of macrophages and neutrophils by chemical inducers, and will provide a useful tool for studying the differentiation of leukemic cells and searching for other differentiation inducers.
Kubonishi
I
I
Ohtsuki
Y
Y
Miyagi
T
T
Yoshimoto
S
S
Akagi
T
T
Miyoshi
I
I
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006651:Histocytochemistry; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000276:immunology / Q000473:pathology*; D008854:Microscopy, Electron; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010587:Phagocytosis; D009418:S100 Proteins / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D009418:S100 Proteins; D009580:Nitroblue Tetrazolium; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate