[Effect of different inactivating agents on microsomal cytochromes P-450 and P-448].
The properties of tert-butanol, sodium deoxycholate, guanidine and mersalyl to convert microsomal cytochromes P-450 and P-448 into enzymatically inactive form P-420 were studied under comparable conditions. Phenobarbital-induced cytochrome P-450 was especially sensitive to the influence of all the agents studied, while cytochrome P-450 from untreated animals was the least sensitive; the 3-methyl-cholanthrene-induced cytochrome P-448 exhibited the least sensitivity to guanidine. The dynamics of changes in content of active and inactive hemoprotein forms after in vitro treatment with the suitable agent might serve as a criterion for estimation whether the phospholipid or protein component of microsomal membrane was damaged.
Poliakova
N E
NE
Gromova
O A
OA
Tsyrlov
I B
IB
D000818:Animals; D000440:Butanols / Q000494:pharmacology; D019388:Cytochrome P-450 CYP1A2; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D003580:Cytochromes / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D003840:Deoxycholic Acid / Q000494:pharmacology; D004790:Enzyme Induction; D004795:Enzyme Stability; D008297:Male; D008565:Membrane Proteins / Q000037:antagonists & inhibitors; D008634:Mersalyl / Q000494:pharmacology; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D020002:tert-Butyl Alcohol
D000440:Butanols; D065607:Cytochrome P-450 Enzyme Inhibitors; D003580:Cytochromes; D008565:Membrane Proteins; D003840:Deoxycholic Acid; D008634:Mersalyl; D003577:Cytochrome P-450 Enzyme System; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2; D020002:tert-Butyl Alcohol
Vliianie razlichnykh inaktiviruiushchikh agentov na mikrosomnye tsitokhromy P-450 i P-448.