Expression of HLA-DR antigens on epithelium associated with lymphoid tissue in the human gastrointestinal tract.
Mucosa from human stomach, terminal ileum, appendix, and colon was studied for epithelial HLA-DR expression using an immunoperoxidase technique with a monoclonal antibody that detects HLA-DR antigens in paraffin embedded tissues. Expression of HLA-DR by epithelial cells was studied with particular reference to the effect of adjacent lymphoid tissue or surrounding chronic inflammation. In the stomach, epithelial HLA-DR appeared to be induced by chronic inflammation. Where lymphoid nodules were present only that epithelium directly adjacent to the lymphoid tissue expressed HLA-DR. Expression was independent of cell type. Epithelium adjacent to normal lymphoid tissue in the terminal ileum, appendix, and colon also expressed HLA-DR, the relationship between expression and proximity to lymphoid tissue being remarkably precise. Expression of HLA-DR by gastrointestinal epithelium appears to be an effect of adjacent lymphocytes, whether part of an inflammatory response or normal tissue. This must be taken into account when assessing HLA-DR expression by gastrointestinal epithelium.
Spencer
J
J
Finn
T
T
Isaacson
P G
PG
D001065:Appendix / Q000276:immunology; D003106:Colon / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005753:Gastric Mucosa / Q000276:immunology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007082:Ileum / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D010581:Peyer's Patches / Q000276:immunology
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II