title: Products of two common alleles at the locus for human placental alkaline phosphatase differ by seven amino acids.
abstract: Amino-terminal amino acid sequences (42 residues) were determined for the products of the three common alleles at the human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] gene locus. The sequences differ at position 3, which is proline in types 1 and 2 but is leucine in type 3. cDNA libraries were constructed in phage lambda gt11 and used to isolate clones covering the coding regions of types 1 and 3 cDNAs. Comparison of the deduced amino acid sequences of the types 1 and 3 proteins showed 7 differences out of 513 amino acids, each due to a single base substitution. cDNA sequence comparisons showed three silent substitutions in the coding regions and three base differences in the greater than 1 kilobase pairs of 3' untranslated sequences.
authors.forename: P S
authors.initials: PS
authors.lastname: Henthorn
authors.forename: B J
authors.initials: BJ
authors.lastname: Knoll
authors.forename: M
authors.initials: M
authors.lastname: Raducha
authors.forename: K N
authors.initials: KN
authors.lastname: Rothblum
authors.forename: C
authors.initials: C
authors.lastname: Slaughter
authors.forename: M
authors.initials: M
authors.lastname: Weiss
authors.forename: M A
authors.initials: MA
authors.lastname: Lafferty
authors.forename: T
authors.initials: T
authors.lastname: Fischer
authors.forename: H
authors.initials: H
authors.lastname: Harris
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics*; D000483:Alleles; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D010920:Placenta / Q000201:enzymology; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004247:DNA; D000469:Alkaline Phosphatase