Anomalous behaviour of cathepsin B: dependence of activity and stability on salt concentration.
Effect of salt concentration on the activity and stability of cathepsin B from buffalo spleen has been investigated. Catheptic activity was maximal at a buffer concentration of 5.5 x 10(-3) M. The enzyme was, however, highly unstable at a salt concentration lower than 1.5 x 10(-2) M. The use of 0.02 M sodium phosphate buffer has been suggested for the assay of cathepsin B activity. For storage of the enzyme, however, a 0.1 M sodium phosphate or other buffer of equivalent ionic strength has been recommended.
Khan
M Y
MY
Department of Biochemistry, School of Life Sciences, North Eastern Hill University, Shillong, India.
Ahmad
S
S
D000818:Animals; D002020:Buffaloes; D002021:Buffers; D002401:Cathepsin B / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010710:Phosphates; D012492:Salts; D013154:Spleen / Q000201:enzymology
D002021:Buffers; D010710:Phosphates; D012492:Salts; D002401:Cathepsin B; C018279:sodium phosphate