title: Effects of photoinitiators for the visible-light resin system on hemolysis of dog erythrocytes and lipid peroxidation of their components.
abstract: The visible-light (VL) polymerizing resin system with photoinitiators (photosensitizer and reducing agent) is widely used in modern dentistry. In this system, polymerization is initiated by photochemical reactions between the photosensitizers (aromatic or aliphatic ketones) and reducing agents (tertiary amines) during VL irradiation. In order to simulate the VL resin-induced toxicity in dental pulps, the present investigation was conducted to determine whether hemolysis of dog erythrocytes and lipid peroxidation of their components occur on exposure to a VL resin system containing aromatic (9-fluorenone, benzil) or aliphatic ketones (camphoroquinone) plus tertiary amine [2-(dimethylamino) ethyl methacrylate]. It was demonstrated that the hemolysis and lipid peroxidation with aromatic ketones were markedly higher than with those of aliphatic ketones, even when the latter were used at higher concentrations. It was clear that peroxidation-induced hemolysis occurred and, further, that surface-active complexes between photosensitizers and reducing agents, which are formed by irradiation, promote hemolytic activity due to their hydrophobic interactions. VL resin-induced responses to dental pulps are probably promoted due to the double effects of unpolymerized monomers and of both radicals and surface-active complexes formed during irradiation.
authors.forename: S
authors.initials: S
authors.lastname: Fujisawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kadoma
authors.forename: E
authors.initials: E
authors.lastname: Masuhara
mesh_terms: D000818:Animals; D004285:Dogs; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005449:Fluorenes / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D007659:Ketones / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism*; D008081:Liposomes; D008567:Membranes, Artificial; D008689:Methacrylates / Q000494:pharmacology; D010658:Phenylglyoxal / Q000031:analogs & derivatives / Q000494:pharmacology; D012117:Resins, Synthetic; D012995:Solubility; D013729:Terpenes
chemical_list: D005449:Fluorenes; D007659:Ketones; D008054:Lipid Peroxides; D008081:Liposomes; D008567:Membranes, Artificial; D008689:Methacrylates; D012117:Resins, Synthetic; D013729:Terpenes; C048339:camphoroquinone; C028401:9-fluorenone; D010658:Phenylglyoxal; C049840:2-(dimethylamino)ethyl methacrylate; C006697:benzil