Binding of ethidium bromide causes dissociation of the nucleosome core particle.
The binding of ethidium bromide to chicken erythrocyte core particles results in a step-wise dissociation of the structure that involves the initial release of one copy each of histones H2A and H2B. Quantitation of the dissociated DNA reveals that a critical amount of drug is required for the dissociation. Above the critical value, the dissociation is time dependent, reversible, and independent of DNA concentration.
McMurray
C T
CT
van Holde
K E
KE
D000818:Animals; D002645:Chickens; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium / Q000378:metabolism*; D006657:Histones / Q000032:analysis; D007364:Intercalating Agents / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000032:analysis / Q000378:metabolism*
D006657:Histones; D007364:Intercalating Agents; D009707:Nucleosomes; D004247:DNA; D004996:Ethidium