Preparation of desulphated bovine fibrinopeptide B and demonstration of its sulphation in vitro by an enzyme system from neuroblastoma-glioma hybrid cells.
A method is described for isolating bovine fibrinopeptide B (bFPB) in a highly purified form from crude bovine fibrinogen, using ion-exchange chromatography on DEAE cellulose. Desulphated bFPB (designated DSbFPB) was prepared by treatment of the product with acid. After incubating DSbFPB with [35S]PAPS, in the presence of a particulate preparation from neuroblastoma-glioma hybrid cells, radioactivity was incorporated into a product identified as [35S]bFPB from its position of elution on reverse-phase HPLC. The possible significance of this observation is discussed.
Barling
P M
PM
Palmer
D J
DJ
Christie
D L
DL
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005340:Fibrinogen / Q000302:isolation & purification*; D005344:Fibrinopeptide A / Q000302:isolation & purification; D005345:Fibrinopeptide B / Q000302:isolation & purification* / Q000378:metabolism; D005910:Glioma / Q000201:enzymology*; D006822:Hybrid Cells / Q000201:enzymology*; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology*; D051381:Rats; D013431:Sulfates; D013466:Sulfurtransferases / Q000378:metabolism
D000596:Amino Acids; D013431:Sulfates; D005344:Fibrinopeptide A; D005345:Fibrinopeptide B; D005340:Fibrinogen; D013466:Sulfurtransferases