title: [Ultrastructure of spermatozoa of rats (Rattus norvegicus). Use of 3 technics in transmission electron microscopy].
abstract: Ultrastructures spermatozoa taken from the seminiferous tubules of rat testes (Rattus norvegicus) were observed under transmission electron microscopy, using the techniques of ultrathin sections, microspreading specimens and replicas. The heads of isolated spermatozoa were of homogeneously high electron density, and had a slightly curved end; in longitudinal sections these zones were composed of a compact homogeneous DNA, covered by a nuclear envelop, cell membrane and small amounts of acrosomal material. The middle piece contained the modified centrioles in the junction zone and flagella microtubules. In transverse sections as well as in replicas, this region consisted of 10 pairs of microtubules and 9 dense fibers surrounded circularly by the mitochondria (mitochondrial sheath).
authors.affiliation: Unidad de Microscopía Electrónica, Universidad de Costa Rica.
authors.forename: F
authors.initials: F
authors.lastname: Ureña
authors.forename: H
authors.initials: H
authors.lastname: Akahori
authors.forename: A
authors.initials: A
authors.lastname: Azofeifa
mesh_terms: D000818:Animals; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D051381:Rats / Q000033:anatomy & histology*; D013094:Spermatozoa / Q000648:ultrastructure*
vernacular_title: Ultraestructura del espermatozoide de rata (Ratus norvegicus). Aplicación de tres técnicas para microscopía electrónica de transmisión.