Chain-shortening of prostaglandin F2 alpha by rat liver peroxisomes.
Liver peroxisomes were isolated from di(2-ethylhexyl)phthalate treated rats by isopycnic sucrose gradient centrifugation of a light mitochondrial fraction. Incubation of prostaglandin F2 alpha with purified peroxisomes resulted in conversion into a more polar product(s). In contrast, incubation with mitochondrial fractions and microsomal fractions under the same conditions did not result in any detectable conversion. The polar material obtained from a preparative incubation was purified by high performance liquid chromatography and characterized by radio-gas chromatography and gas chromatography-mass spectrometry. The structure of the polar compound was shown to be 5,7,11-trihydroxy-tetranorprost-9-enoic acid (tetranor-prostaglandin F1 alpha). Prostaglandin F2 alpha was thus chain-shortened by four carbon atoms.
Diczfalusy
U
U
Alexson
S E
SE
Pedersen
J I
JI
D000818:Animals; D002458:Cell Fractionation; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000378:metabolism*; D011460:Prostaglandins F / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011460:Prostaglandins F; D015237:Dinoprost