Transformation as a tool for studying the epidemiology of tet determinants in Streptococcus pneumoniae.
Transformation of pneumococcus was used to detect homology among tetracycline resistance determinants of clinical isolates of Streptococcus pneumoniae. A strain of pneumococcus containing a mutated tet determinant (tet-3), of class M, integrated into the chromosome was used as a recipient in transformation experiments, where donor DNA was from the tetracycline resistant isolates. 34/34 strains appeared to have tet determinants homologous to tet-3 (i.e. tet M). Still using transformation it was possible to determine that the tet-3 transforming activity of DNA from Tn916 and S. pneumoniae BM6001 was contained in a 5 kb HincII fragment. For this purpose a transformation technique where donor DNA was directly taken from low melting point agarose gels was standardized and used.
Pozzi
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G
Stellini
M
M
Marri
L
L
Molina
A M
AM
D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005819:Genetic Markers; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D005819:Genetic Markers; D013752:Tetracycline