Inhibition of acid production from oral bacteria by fluorapatite-derived fluoride.
The inhibitory effect of fluorapatite (FAP)-derived fluoride upon resting cell suspensions of Streptococcus mutans incubated at pH 4.5 and 6.5 was studied using lactic acid production from 0.1% sucrose as an indicator of fermentation activity. Cells incubated with FAP produced significantly less lactic acid than did cells incubated with hydroxyapatite (HAP). Addition of HAP to cell suspensions containing FAP reduced this inhibition, suggesting that dissolution of the FAP was necessary for inhibition. Incubation with low concentrations of NaF showed significant inhibition in cell suspensions incubated with as little as 0.45 micrograms/mL F at pH 5.0. These results provide further support to the hypothesis that fluoride levels in plaque and enamel, achievable through use of fluoridated water and/or fluoride dentifrices, may produce appreciable inhibition of glycolysis at the acidic pH levels which are readily achieved in plaque. Thus, bacterial acid production may activate plaque and enamel-bound fluoride, resulting in inhibition of further acid production, and thereby contribute substantially to the other cariostatic mechanisms of fluoride.
Harper
D S
DS
Loesche
W J
WJ
D001031:Apatites / Q000494:pharmacology*; D003773:Dental Plaque / Q000378:metabolism / Q000382:microbiology*; D005459:Fluorides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D019344:Lactic Acid; D013295:Streptococcus mutans / Q000378:metabolism*
D001031:Apatites; D007773:Lactates; D019344:Lactic Acid; C025105:fluorapatite; D005459:Fluorides