Comparison of two methods to evaluate drug-cytotoxicity on tumor cell lines cultured in vitro.
Some porphyrin compounds: P-NO2 and CVRIV were screened for cytotoxic activity against HT-29, LOVO, human tumor cell lines. The new radiometric assay was used for all cell lines. The soft agar cloning system was also utilized. The tested compounds decrease the growth index, measured in the radiometric assay, as 14CO2 production, and similarly depress the growth of tumor colonies on soft agar in the clonogenic assay. The cytotoxic effects of the compounds tested by these different methods were analysed statistically and resulted quantitatively similar. Based on these findings the radiometric assay represents a method, simple and rapid, which can be used as the clonogenic assay to screen new anticancer drugs.
Pinelli
A
A
Department of Pharmacology, University of Milan.
Trivulzio
S
S
Von Hoff
D D
DD
Warfel
L
L
D002250:Carbon Radioisotopes; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D011166:Porphyrins / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D002250:Carbon Radioisotopes; D011166:Porphyrins; D005947:Glucose