title: Purification of a 32.5 kDa monomeric sulfotransferase from rat liver with activity for bile acids and phenolic steroids.
abstract: Both bile acid and phenolic steroid sulfotransferase activities in rat liver cytosol have previously been identified in fractions corresponding to apparent molecular masses of 60-70 and 30-35 kDa. We purified the latter activity corresponding to a monomeric protein. Activity for bile acids and phenolic steroids co-eluted on sequential chromatography on Sephadex G-75 sf, Affigel blue, chromatofocusing and hydroxyapatite. The protein was homogeneous on SDS-PAGE (32.5 kDa).
authors.forename: H
authors.initials: H
authors.lastname: Takikawa
authors.forename: A
authors.initials: A
authors.lastname: Stolz
authors.forename: N
authors.initials: N
authors.lastname: Kaplowitz
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); D000818:Animals; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology; D004958:Estradiol / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000302:isolation & purification* / Q000378:metabolism; D013658:Taurolithocholic Acid / Q000378:metabolism
chemical_list: D004958:Estradiol; D013658:Taurolithocholic Acid; D015096:3-Hydroxysteroid Dehydrogenases; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); D013466:Sulfurtransferases; D015238:Sulfotransferases; C019786:bile-salt sulfotransferase; C044251:steroid sulfotransferase