Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization.
A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86-88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity, suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2----q11.2), although a secondary site of hybridization at q24----25 was also observed.
Liou
G I
GI
Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030.
Fong
S L
SL
Gosden
J
J
van Tuinen
P
P
Ledbetter
D H
DH
Christie
S
S
Rout
D
D
Bhattacharya
S
S
Cook
R G
RG
Li
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D012177:Retinol-Binding Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D000099214:Retinol-Binding Proteins, Interstitial
D004247:DNA; D005136:Eye Proteins; D012333:RNA, Messenger; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial