title: Interaction of plasma membrane-associated filaments and H-2 histocompatibility antigens before and after induced patching and capping.
abstract: The interaction of H-2 antigens and plasma membrane-associated filaments was studied on dry-cleaved preparations of immunogold-labelled lymphoma cells. In prefixed cells, the plasma membrane-associated network was isotropic without any prevailing direction of the filaments, and the gold-labelled H-2 antigens were preferentially localized over or at a very short distance from membrane-associated filaments. Incubation of unfixed cells with anti-H-2 antibodies followed by fixation and incubation with anti-Ig, did not induce detectable redistribution of H-2 antigens or of the filament network. Notwithstanding this apparent absence of rearrangement of H-2 antigens and filaments, a detergent-resistant linkage to the cytoskeleton was induced. Before immune incubations, virtually all H-2 antigens were solubilized by extraction with Triton X-100, while after incubation with anti-H-2 antibodies about 50% of the H-2 antigens were linked to the Triton X-100-insoluble cytoskeleton. Sequential addition of anti-H-2 and anti-Ig antibodies to unfixed cells induced formation of patches and caps of H-2 antigens. Under these conditions, the majority of the H-2 antigens became linked to the detergent-resistant cytoskeleton. Redistribution into patches and caps was often accompanied by a local rearrangement of the isotropic network into bundles of parallel filaments immediately adjacent to the plasma membrane. Patches were seen to overly both isotropic networks and these parallel filaments. Large sheets of plasma membrane overlying parallel filaments were frequently devoid of gold-labelled H-2 antigens and coated pits, and thus most probably represented areas away from caps. This observation suggests that capping is accompanied by a rearrangement of filaments close to the membrane.
authors.affiliation: Division of Cell Biology, The Netherlands Cancer Institute (Antoni van Leeuwenhoek Huis), Amsterdam.
authors.forename: C A
authors.initials: CA
authors.lastname: Feltkamp
authors.forename: H
authors.initials: H
authors.lastname: Spiele
authors.forename: E
authors.initials: E
authors.lastname: Roos
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology*; D007150:Immunohistochemistry; D007942:Leukemia, Experimental / Q000473:pathology; D051379:Mice; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured
chemical_list: D006183:H-2 Antigens