Induction of differentiation of human myeloid cell lines by tumor necrosis factor in cooperation with 1 alpha,25-dihydroxyvitamin D3.
We analyzed the combined effect of tumor necrosis factor and 1 alpha,25-dihydroxyvitamin D3 on the differentiation of human myeloid cell lines HL-60, ML3, and U937. The two compounds synergize in inducing the morphological, phenotypic, enzymatic, and functional characteristics of cells of the monocytic lineage. Immune gamma-interferon synergizes with each compound to induce differentiation. However, recombinant tumor necrosis factor is much more effective than recombinant gamma-interferon in potentiating the effect of 1 alpha,25-dihydroxyvitamin D3 and, alone, is also more effective than recombinant gamma-interferon in inducing expression of the high-affinity Fc receptor on ML3 cells. The possible physiological or pathological relevance of the synergistic effect of tumor necrosis factor and 1 alpha,25-dihydroxyvitamin D3 on monocytic differentiation is discussed.
Trinchieri
G
G
Rosen
M
M
Perussia
B
B
D002117:Calcitriol / Q000494:pharmacology*; D002454:Cell Differentiation; D002460:Cell Line; D004357:Drug Synergism; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D010641:Phenotype; D011961:Receptors, Fc / Q000378:metabolism; D013499:Surface Properties; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D011961:Receptors, Fc; D014409:Tumor Necrosis Factor-alpha; D002117:Calcitriol