[Staphylococcal enterotoxin type D: isolation, purification, identification].
The three step scheme of isolation and purification of staphylococcal type D enterotoxin was elaborated to obtain the homogeneous protein. The scheme includes protein concentration by (NH4)2SO4 saturation and subsequent fractionation on DEAE-cellulose and Sephacryl S-200. The yield of homogeneous protein is 25.5%. The molecular mass of the 29,600 D protein was identified by electrophoresis in polyacrylamide gel in the presence of SDS. The enterotoxic dose for staphylococcal type D enterotoxin is 5 mkg per kg of body mass as identified in experiments with cats. Immunological identity of the obtained protein was established to the commercial preparation of staphylococcal enterotoxin D produced by "Serva".
Naubet'iarov
R B
RB
Beĭlbaeva
M L
ML
Ezepchuk
Iu V
IuV
D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D004768:Enterotoxins / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D013211:Staphylococcus aureus / Q000032:analysis*
D004768:Enterotoxins; C019368:enterotoxin D, Staphylococcal
Stafilokokkovyĭ énterotoksin tipa D: vydelenie, ochistka, identifikatsiia.