title: Monoclonal antibody to an intracellular antigen images human melanoma transplants in nu/nu mice.
abstract: Mouse monoclonal antibody TA99 detects a 70-kDa pigmentation-associated glycoprotein in human melanoma cell lines. The antigen cannot be detected on the cell surface by sensitive rosetting techniques or absorption studies, nor can it be detected as a secreted product in culture fluids. Contrary to expectation, 125I-labeled TA99 specifically localized to pigmented human melanoma transplants in nu/nu mice; no localization to nonpigmented melanoma or control tumors was found. Tumor imaging was initially obscured by circulating 125I-labeled TA99 during the first 6 days after antibody injection. With clearance of 125I-labeled TA99 from the blood (half-life, 4-7 days), specific tumor images could be clearly defined by day 13. Due to the persistence of 125I-labeled TA99 at the tumor site (8.9% of the injected dose at 1 week and 4.6% at 8-10 weeks), images were obtainable for up to 10 weeks. At 8-10 weeks, the tumor/blood ratio was 10(4)-10(5), and the tumor/normal tissue ratio ranged from 10(2) to 10(5). In view of these findings, antibodies detecting intracellular antigens may have a role in tumor imaging and therapy.
authors.forename: S
authors.initials: S
authors.lastname: Welt
authors.forename: M J
authors.initials: MJ
authors.lastname: Mattes
authors.forename: R
authors.initials: R
authors.lastname: Grando
authors.forename: T M
authors.initials: TM
authors.lastname: Thomson
authors.forename: R W
authors.initials: RW
authors.lastname: Leonard
authors.forename: P B
authors.initials: PB
authors.lastname: Zanzonico
authors.forename: R E
authors.initials: RE
authors.lastname: Bigler
authors.forename: S
authors.initials: S
authors.lastname: Yeh
authors.forename: H F
authors.initials: HF
authors.lastname: Oettgen
authors.forename: L J
authors.initials: LJ
authors.lastname: Old
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm / Q000032:analysis*; D002460:Cell Line; D006801:Humans; D007457:Iodine Radioisotopes; D008545:Melanoma / Q000000981:diagnostic imaging / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D014183:Transplantation, Heterologous
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D007457:Iodine Radioisotopes