Rapid gas-chromatographic assay of bupivacaine in plasma.
A method for estimating bupivacaine concentration in human plasma by capillary gas-chromatography using solid injection and nitrogen-specific detection is described. Etidocaine, another anilidetype local anesthetic was used as internal standard and added to the sample before single-step extraction with diethylether. This method demonstrates high sensitivity (6 ng/ml plasma) and combines selectivity, rapidity, and simplicity. Results of this procedure correlate well with those obtained by an HPLC method.
Le Normand
Y
Y
Département de Pharmacologie, Faculté de Médecine, Centre Hospitalier Universitaire, Hôpital Laennec, Nantes, France.
Athouel
A
A
Blanloeil
Y
Y
De Villepoix
C
C
Melchior
J C
JC
Kergueris
M F
MF
Bourin
M
M
Larousse
C
C
D002045:Bupivacaine / Q000097:blood* / Q000493:pharmacokinetics; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans
D002045:Bupivacaine