Supernatants from ethanol-containing macrophage cultures have cytotoxic activity.
After macrophages are cultured in the presence of ethanol (0.1-2.0 mg/ml) for 72 hr, the culture supernatant contains one or more cytotoxic factors which cause the detachment of mouse A9 tumour cells and a reduction of 3H-thymidine incorporation by K562 cells. The cytotoxic activity is largely abolished by heating to 56 degrees C for 10 min and is non-dialysable and hence associated with material having a molecular weight greater than about 10,000. These in vitro data support the possibility that alcohol-induced tissue damage may result from the action of cytotoxic factors generated as a consequence of an interaction between ethanol and tissue macrophages in vivo.
Wickramasinghe
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D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D003956:Dialysis; D000431:Ethanol / Q000633:toxicity*; D006358:Hot Temperature; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000431:Ethanol; D013936:Thymidine