Frame-shift mutants induced by quinacrine are recognized by the mismatch repair system in Streptococcus pneumoniae.
We describe the isolation of amethopterin-resistant mutants induced by quinacrine treatment of exponentially growing cultures of Streptococcus pneumoniae. Only mutants located by recombination analysis in a few hundred base pairs were further studied. They were cloned and their DNA sequences show that most of them are +/-1-base frame-shift mutants. They are excised and repaired to a degree similar to transition mutants (low efficiency class), suggesting that the mismatches resulting from a transition or a +/-1-base mutation are similar substrates for the Hex mismatch repair system.
Gasc
A M
AM
Sicard
A M
AM
D001483:Base Sequence; D002872:Chromosome Deletion; D004260:DNA Repair; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D008727:Methotrexate / Q000494:pharmacology; D009154:Mutation; D011796:Quinacrine / Q000494:pharmacology*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*; D014169:Transformation, Bacterial
D011796:Quinacrine; D008727:Methotrexate