Regulation of angiotensin II binding sites in the subfornical organ and other rat brain nuclei after water deprivation.
1. Binding sites for angiotensin II have been localized in forebrain and brain-stem areas of water-deprived and control Sprague-Dawley rats, employing autoradiography with computerized microdensitometry. 2. Angiotensin II receptor sites were identified in the organum vasculosum of the lamina terminalis, subfornical organ, paraventricular nucleus, median preoptic nucleus, area postrema, nucleus of the solitary tract, and inferior olive. 3. After dehydration a significant increases in the concentration of angiotensin II receptors was detected only in the subfornical organ. Although there was an increased concentration of angiotensin II binding sites in the organum vasculosum of the lamina terminalis, the median preoptic nucleus, and the paraventricular nucleus after dehydration, these changes did not reach statistical significance. Other brain nuclei investigated did not show differences in angiotensin II binding sites in the dehydrated rats compared to controls. 4. These results indicate that angiotensin II receptors in the subfornical organ may play an important role in fluid homeostasis during dehydration.
Nazarali
A J
AJ
Section on Clinical Pharmacology, National Institute of Mental Health, Bethesda, Maryland 20892.
Gutkind
J S
JS
Saavedra
J M
JM
D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001835:Body Weight; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009490:Neurosecretory Systems / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000378:metabolism*; D013356:Subfornical Organ / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014868:Water Deprivation
D011945:Receptors, Angiotensin; D000804:Angiotensin II