title: Enhanced casein kinase II activity during mouse embryogenesis. Identification of a 110-kDa phosphoprotein as the major phosphorylation product in mouse embryos and Krebs II mouse ascites tumor cells.
abstract: Mouse embryos at various stages of development were used to study the relationship of protein kinase activities with normal embryogenesis. Casein kinase II (CKII) activity in developing mouse embryos shows a 3-4-fold activity increase at day 12 of gestation. Together with the CKII activity, increased phosphorylation of a 110-kDa protein is observed. Treatment of the embryo extracts with heparin, a highly specific inhibitor of CKII activity, results in a drastic reduction of the 110-kDa protein phosphorylation indicating that the protein might be a CKII-specific substrate. Rapidly proliferating mouse tumour cells also show an enhanced CKII activity. Here too, a 110-kDa phosphoprotein was the major phosphoryl acceptor. Partial proteolytic digestion shows that both proteins are identical. Other protein kinases tested (cAMP- and cGMP-dependent protein kinases) only show a basal level of enzyme activity with minor alterations throughout the different stages of embryogenesis investigated.
authors.forename: H R
authors.initials: HR
authors.lastname: Schneider
authors.forename: G H
authors.initials: GH
authors.lastname: Reichert
authors.forename: O G
authors.initials: OG
authors.lastname: Issinger
mesh_terms: D000818:Animals; D001345:Autoradiography; D002287:Carcinoma, Krebs 2 / Q000201:enzymology*; D047388:Casein Kinases; D002499:Centrifugation, Density Gradient; D004622:Embryo, Mammalian / Q000201:enzymology*; D005314:Embryonic and Fetal Development; D051379:Mice; D008970:Molecular Weight; D010449:Peptide Mapping; D010750:Phosphoproteins / Q000302:isolation & purification*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*
chemical_list: D010750:Phosphoproteins; D011494:Protein Kinases; D047388:Casein Kinases