Involvement of prostaglandins in the immune alterations caused by the exposure of mice to ultraviolet radiation.
Our study was designed to analyze the possible involvement of prostaglandins in the mechanisms responsible for the depressions in contact hypersensitivity (CH) responsiveness observed in UVR-exposed animals. Low-dose UVR-exposed animals were found to exhibit a depressed capacity to elicit CH responses after hapten application to irradiated (devoid of Langerhans cells) or UVR-protected (normal Langerhans cells) dorsal skin surfaces. Normal responsiveness was observed in low-dose UVR-exposed animals sensitized through unirradiated ventral skin surfaces. Indomethacin treatment of low-dose UVR-exposed animals (to inhibit prostaglandin synthesis in vivo) caused a retention in the capacity to respond normally to CH induction to haptens applied to the nonirradiated, but not to irradiated, dorsal skin surfaces. High-dose UVR-exposed animals, which normally exhibit a depression in responsiveness to hapten sensitization, retained a normal capacity to elicit CH responses if treated with the drug indomethacin. These findings implicate prostaglandins in the pathogenesis of the immunologic hyporesponsiveness, observed in low- and high-dose UVR-exposed animals. Our studies also determined that under all experimental conditions where animals were contact sensitized through nonirradiated skin sites, CH-effector cells could be found in the draining lymph nodes. No CH-effector cells were observed in the lymph nodes of mice that were contact sensitized directly through irradiated skin sites. It was also found that the spleens of both UVR-exposed and normal animals contained adoptively transferrable suppressor cells subsequent to hapten application. This demonstration of CH-effector and CH-suppressor cells in both normal and UVR-exposed animals did not directly relate to the potential of the donor animals to elicit a CH response.
Chung
H T
HT
Burnham
D K
DK
Robertson
B
B
Roberts
L K
LK
Daynes
R A
RA
D000818:Animals; D003877:Dermatitis, Contact; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007116:Immunization, Passive; D007165:Immunosuppression Therapy; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011453:Prostaglandins / Q000276:immunology*; D012867:Skin / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D000949:Histocompatibility Antigens Class II; D011453:Prostaglandins; D007213:Indomethacin