The use of NaOH as transfer solution of DNA onto nylon membrane decreases the hybridization efficiency.
Rigaud
G
G
Grange
T
T
Pictet
R
R
D004247:DNA / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D009693:Nucleic Acid Hybridization; D009757:Nylons; D012972:Sodium Hydroxide; D012996:Solutions
D009757:Nylons; D012996:Solutions; D012972:Sodium Hydroxide; D004247:DNA