title: Molecular approaches to genome analysis: a strategy for the construction of ordered overlapping clone libraries.
abstract: Here we describe progress on a series of molecular techniques designed to bridge the gap between genetic and molecular distances in mammals. This is an essential step in the molecular cloning of genes defined by mammalian mutations, and in the molecular analysis of large regions of mammalian genomes. We summarize approaches for the physical and molecular analysis of genetic distances and describe the experimental, statistical and computational basis of a new approach to create ordered libraries of overlapping clones from large genomes.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, FRG.
authors.forename: F
authors.initials: F
authors.lastname: Michiels
authors.forename: A G
authors.initials: AG
authors.lastname: Craig
authors.forename: G
authors.initials: G
authors.lastname: Zehetner
authors.forename: G P
authors.initials: GP
authors.lastname: Smith
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
mesh_terms: D001499:Bayes Theorem; D003001:Cloning, Molecular; D005544:Forecasting; D007255:Information Services; D008967:Molecular Biology; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D009841:Oligonucleotides
chemical_list: D009841:Oligonucleotides