Sister chromatid exchange in highly purified human B and T lymphocytes.
Sister chromatid exchange (SCE) rates were determined in human peripheral blood B and T lymphocyte populations highly purified by immunologic methods. The purified populations were supplemented with gamma-irradiated unseparated autologous mononuclear cells to restore helper-functions and stimulated with pokeweed mitogen (PWM) and phytohemagglutinin (PHA), respectively. Measured at the different peaks of proliferation after identical bromodeoxyuridine (BrdU) incubation times, T lymphocytes showed significantly higher SCE frequencies than B lymphocytes. In both populations, different proliferation kinetics and a different minimal BrdU concentration for sister chromatid differentiation (SCD) were observed.
D001402:B-Lymphocytes / Q000648:ultrastructure*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D004260:DNA Repair; D006801:Humans; D012854:Sister Chromatid Exchange; D013601:T-Lymphocytes / Q000648:ultrastructure*
D001973:Bromodeoxyuridine