Lymphotoxin is an important T cell-derived growth factor for human B cells.
Two different assays for B cell growth factors (BCGF) and an antibody against lymphotoxin were used to show that the presence of lymphotoxin in conditioned media derived from normal activated T cells and in a partially purified BCGF accounts for a substantial portion of their B cell growth-promoting activity. A competitive binding assay confirmed the presence of significant amounts of lymphotoxin in the partially purified BCGF. Recombinant lymphotoxin enhanced the proliferation of activated B cells and augmented B cell proliferation and immunoglobulin secretion induced by interleukin-2.
D000917:Antibody Formation / Q000187:drug effects; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D015847:Interleukin-4; D007378:Interleukins; D008213:Lymphocyte Activation; D008233:Lymphotoxin-alpha / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000502:physiology*
D006133:Growth Substances; D007378:Interleukins; D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins; D015847:Interleukin-4; D004247:DNA