Von Willebrand factor fragment in type IIA von Willebrand's disease: demonstration of two different forms of fragments.
A fast-migrating precipitin peak which showed a reaction of partial immunochemical identity with the major von Willebrand factor (vWf) component was detected by crossed immunoelectrophoresis in agarose gel in plasma but not in platelets from a patient with type IIA von Willebrand's disease (vWD). Another patient undergoing urokinase therapy had a vWf fragment in plasma which was antigenically cross-reactive with the major vWf component. When plasma from both patients was mixed and electrophoresed together in the first dimension, two fast-moving precipitin arcs were demonstrated in the second dimension. These data indicate that two different kinds of vWf fragments can be generated in vivo.
D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D009203:Myocardial Infarction / Q000097:blood / Q000188:drug therapy; D010446:Peptide Fragments / Q000097:blood*; D014568:Urokinase-Type Plasminogen Activator / Q000627:therapeutic use; D014842:von Willebrand Diseases / Q000097:blood*; D014841:von Willebrand Factor / Q000032:analysis*
D010446:Peptide Fragments; D014841:von Willebrand Factor; D014568:Urokinase-Type Plasminogen Activator