Reticulomyxa: a new model system of intracellular transport.
Reticulomyxa is a large multinucleated freshwater protozoan that provides a new model system in which to study intracellular transport and cytoskeletal dynamics. Within the cell body and reticulopodial network, rapid, visually striking saltatory organelle motility as well as bulk cytoplasmic streaming can be readily observed. In addition, the cytoskeletal elements within these strands undergo dynamic splaying and fusing rearrangements, which can be visualized by video-enhanced light microscopy. A reactivatable lysed cell model has been developed that appears to preserve, and therefore permits examination of, these three forms of motility in a more controlled environment. Individual organelle movements are microtubule-based and have similarities to, but also differences from, the recently described kinesin-based transport. This lysed cell model can be further manipulated to provide native, ordered, completely exposed networks of either microtubules or microfilaments, or a combination of both, and thus may serve as a versatile motility assay system in which to examine the movement of exogenously added isolated organelles or latex beads.
D008841:Actin Cytoskeleton / Q000502:physiology; D000818:Animals; D003595:Cytoplasmic Streaming; D003599:Cytoskeleton / Q000502:physiology*; D056890:Eukaryota / Q000502:physiology / Q000648:ultrastructure*; D008870:Microtubules / Q000502:physiology; D008954:Models, Biological; D009068:Movement; D009940:Organoids / Q000502:physiology*