title: In vitro effect of vasoactive intestinal polypeptide and peptide histidine isoleucine on prolactin secretion by the bullfrog pituitary gland.
abstract: The effects of vasoactive intestinal polypeptide (VIP) and peptide histidine isoleucine (PHI) on the in vitro secretion of prolactin (PRL) by the pituitary gland of bullfrogs (Rana catesbeiana) were studied. Pituitary glands were incubated in 67% Eagle's minimum essential medium containing [3H]leucine in the presence or absence of VIP (10(-8)-10(-6) M) or PHI (10(-7)-10(-6) M). Prolactin in the medium and pituitaries was measured by a homologous radioimmunoassay. In addition, PRL synthesis during incubation was monitored by measuring radioactivity of [3H]leucine incorporated into PRL in the medium and pituitaries. Both peptides stimulated release and synthesis of PRL in vitro.
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D007700:Kinetics; D010451:Peptide PHI / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D011892:Rana catesbeiana; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D010451:Peptide PHI; D014660:Vasoactive Intestinal Peptide; D011388:Prolactin