Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein.
A 4-kilobase complementary DNA (cDNA) encoding human macrophage-specific colony-stimulating factor (CSF-1) was isolated. When introduced into mammalian cells, this cDNA directs the expression of CSF-1 that is structurally and functionally indistinguishable from the natural human urinary CSF-1. Direct structural analysis of both the recombinant CSF-1 and the purified human urinary protein revealed that these species contain a sequence of at least 40 amino acids at their carboxyl termini which are not found in the coding region of a 1.6-kilobase CSF-1 cDNA that was previously described. These results demonstrate that the human CSF-1 gene can be expressed to yield at least two different messenger RNA species that encode distinct but related forms of CSF-1.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000235:genetics* / Q000652:urine; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008264:Macrophages / Q000502:physiology; D008970:Molecular Weight; D010446:Peptide Fragments; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics
D003115:Colony-Stimulating Factors; D010446:Peptide Fragments; D012333:RNA, Messenger; D004247:DNA