Detection of immuno-complex formation via surface plasmon resonance on gold-coated diffraction gratings.
The sensitivity of surface plasmon resonance techniques to changes in local interfacial refractive index has been exploited to detect immuno-complex formation in two model biochemical systems. A gold-coated diffraction grating has been used to excite surface plasmons at the gold/solution interface to which either human immunoglobulin G or the immunoglobulin fraction of sheep antiserum to human serum albumin was physically adsorbed. The complementary proteins, either affinity purified goat anti-human-IgG IgG or human serum albumin was subsequently specifically bound by immuno-complex formation. The binding reactions could be followed with respect to time.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D006046:Gold; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D066298:In Vitro Techniques; D055095:Optics and Photonics; D012709:Serum Albumin / Q000276:immunology / Q000378:metabolism
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D012709:Serum Albumin; D006046:Gold