Induction of gastrulation in the chick embryo.
Interaction between the epiblast and the primary hypoblast in chick blastula results in induction of the primitive streak (PS) in the epiblast. Alpha-amanitin, a specific inhibitor of poly A-containing RNA synthesis, inhibits formation of the definitive PS. This inhibition is associated with qualitative changes in the pattern of protein synthesis in the hypoblast but not in the epiblast. The protein pattern of the component areas of the epiblast shows increase in some polypeptides after treatment with alpha-amanitin. By contrast, alpha-amanitin resulted in a decrease in synthesis of several polypeptides, which are either undetectable or weakly present in the hypoblast. The alpha-amanitin-sensitive translational products of the embryonic genome that are observed in the hypoblast may have specific functions in the control of PS induction and stabilization.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002642:Chick Embryo / Q000254:growth & development* / Q000378:metabolism; D004627:Embryonic Induction / Q000187:drug effects; D005775:Gastrula / Q000033:anatomy & histology* / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D014176:Protein Biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects
D000546:Amanitins