Low burst promoting activity production by phytohemagglutinin-stimulated mononuclear cells of cord blood.
Burst-promoting activity (BPA) produced by phytohemagglutinin(PHA)-stimulated cord blood mononuclear cells (MNC) was examined using the two-stage cell culture assays. Burst-promoting activity was measured as the increase in the number of early erythroid progenitor cells in 2-day incubation of peripheral blood MNC with or without the conditioned medium of PHA-stimulated MNC (PHA-LCM). Burst-promoting activity in PHA-LCM of cord blood was significantly lower than that of adult blood (37 +/- 13 versus 105 +/- 19%, mean +/- SD, p less than 0.01). No elevation of inhibitors to the erythroid colony growth was noted in PHA-LCM of cord blood. In contrary, the response of cord blood MNC to PHA was similar to that of adult blood MNC, as determined by colony-stimulating activity production and cell proliferation. These results showed that burst-promoting activity production by PHA-stimulated MNC of cord blood was lower than that of adult blood.
D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000097:blood; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D004920:Erythropoiesis; D005312:Fetal Blood / Q000378:metabolism / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007231:Infant, Newborn; D009000:Monocytes / Q000378:metabolism / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013936:Thymidine / Q000097:blood
D003115:Colony-Stimulating Factors; D003470:Culture Media; D010835:Phytohemagglutinins; D013936:Thymidine