title: Mutation at the APRT locus in Friend erythroleukaemia cells. 1. Mutation rates and properties of mutants.
abstract: Spontaneous mutation at the adenine phosphoribosyl transferase (APRT) locus in clone 707 of the Friend cell line was examined. The frequency of cells resistant to 2,6-diaminopurine (DAP) was found to be 2.6 X 10(-5) with a mutation rate of 1.81 X 10(-6) cell-1 generation-1. APRT activities in 9 DAP-resistant clones were found to vary between 0 and 27% the level observed in wild-type cells. It is suggested that clone 707 cells are heterozygous or functionally hemizygous at the APRT locus.
mesh_terms: D000228:Adenine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology; D007942:Leukemia, Experimental / Q000201:enzymology*; D051379:Mice; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*
chemical_list: D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase