Detection of receptors for murine B cell stimulatory factor 1 (BSF1): presence of functional receptors on CBA/N splenic B cells.
The presence of receptors specific for murine B cell stimulatory factor 1 (BSF1) was demonstrated by utilizing an internally radiolabeled recombinant BSF1. Radiolabeled BSF1 was efficiently produced in Xenopus laevis oocytes injected with a cloned mRNA for BSF1 and 35S-methionine. The labeled BSF1 specifically bound to splenic B cells. A Scatchard analysis indicated the existence of one class of receptor sites. BSF1 receptors were found to be distributed on a wide range of hematopoietic lineage cells, including B cells, T cells, macrophages, and mast cells. B cells from CBA/N mice with the xid gene defect had a similar level of BSF1 binding capacity compared with BALB/c strain B cells, and responded well to insoluble anti-Ig and BSF1 in proliferation assays, indicating that CBA/N B cells express functional BSF1 receptors at normal levels. Pre-B cell lines showed low levels of BSF1 binding, suggesting that cells in the B cell lineage acquire BSF1 responsiveness early in development.
D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis*; D002460:Cell Line; D006133:Growth Substances / Q000378:metabolism*; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D015847:Interleukin-4; D007962:Leukocytes / Q000032:analysis; D008222:Lymphokines / Q000378:metabolism*; D008264:Macrophages / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008808:Mice, Inbred CBA / Q000276:immunology*; D008817:Mice, Mutant Strains / Q000276:immunology*; D009928:Organ Specificity; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen / Q000032:analysis*; D011994:Recombinant Proteins / Q000378:metabolism; D013154:Spleen / Q000166:cytology*
D006133:Growth Substances; D008222:Lymphokines; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen; D011994:Recombinant Proteins; D015847:Interleukin-4