[Phenotyping of peripheral lymphocytes with monoclonal antibodies in newborn infants and adults].
The authors investigated and compared lymphocyte subsets natural killer in 33 newborns and in 33 healthy adults. From 5 ml of heparinized venous blood were separated mononuclear cells on density-gradient Ficoll-Hypaque. The vitality was determined with immunofluorescent method (orange acridine and ethiobromide). It was positive if resulted at least 95%. Lymphocyte phenotype was also examined after incubation with monoclonal antiserum Becton-Dickinson conjugated with FITC and PE for 45' at 4 degrees C. Two hundred cells were counted on each slide with fluorescent microscopy. Total number of T-lymphocyte (Leu-4) was increased in newborns; total B-cells (Leu-12) were similar in newborns and healthy adults; T-helper/T-suppressor (Leu-3a/Leu-2a) ratio was above the normal range for increase T-helper. The percentage of subsets with double marker Leu-2a+/Leu-3a+ was always about 15-20%. NK cells with phenotype HNK-1 (Leu-7) were a little number in newborns while the percentage of NK-cells-Leu-11a was similar among adults and newborns.
D000328:Adult; D000375:Aging / Q000276:immunology; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000145:classification; D005260:Female; D006801:Humans; D007231:Infant, Newborn / Q000276:immunology*; D008214:Lymphocytes / Q000145:classification*; D008297:Male; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification
D000911:Antibodies, Monoclonal
Fenotipizzazione linfocitaria periferica con anticorpi monoclonali nel neonato e nell'adulto.