title: Immunoblot analysis of antigens associated with Haemophilus ducreyi using serum from immunised rabbits.
abstract: Sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting were used to characterise isolates of Haemophilus ducreyi. Isolates of H ducreyi were heterogeneous in protein composition, but isolates from single outbreaks appeared similar both in protein profiles and antigenic analysis. Rabbits immunised with H ducreyi responded with a vigorous humoral immune response in which multiple antigenic polypeptides were detected. The most prominent antigens had molecular masses of 67, 42, 22.5, and 20 kilodaltons.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006191:Haemophilus ducreyi / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial