Purification of immunotoxins containing ricin A-chain and abrin A-chain using blue sepharose CL-6B.
We describe a method for separating antibody from immunotoxins by affinity chromatography on Cibacron blue F3GA coupled to Sepharose (Blue Sepharose). The antibody did not bind to the gel. The immunotoxins were bound by their ricin A-chain or abrin A-chain moiety and could be recovered in high yield and purity using mild elution conditions. The method is suitable for the large-scale purification of immunotoxins.
D000036:Abrin / Q000302:isolation & purification*; D000906:Antibodies / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D000922:Immunotoxins / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*; D012276:Ricin / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
D000906:Antibodies; D000922:Immunotoxins; D010940:Plant Proteins; C017842:cibacron blue-sepharose; D000036:Abrin; D012276:Ricin; D012685:Sepharose