Specificity of the immune response of mice to herpes simplex virus glycoproteins B and D constitutively expressed on L cell lines.
Mouse L cell lines have been developed which constitutively express glycoproteins B (gB) and D (gD) of herpes simplex virus type 1. When used to study the immune response of mice to the viral glycoproteins, it was found that both gB and gD induce a delayed type hypersensitivity response and both also induce an antibody response, but only the cell line expressing gD could stimulate the production of neutralizing antibody. Virus-specific cytotoxic T lymphocytes (CTLs) recognized gB expressed by the cell line and this line could also induce CTLs in mice. Recognition of gD by major histocompatibility complex class I restricted CTLs was never seen. Vaccination of mice with the cell lines provided protection from viral challenge and inhibited the establishment of a latent infection, although gD proved to be the better protective immunogen.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D005796:Genes; D006968:Hypersensitivity, Delayed; D007114:Immunization; D007739:L Cells; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C039447:glycoprotein D, Human herpesvirus 1