Antigen presenting cells in human decidual tissue. II: Accessory cells for the development of anti-trinitrophenyl cytotoxic T lymphocytes.
Antigen presenting cells were isolated from early human decidua or peripheral blood by elution with EDTA of cells that after Ficoll-Paque separation bear receptors for, and have bound to, fibronectin. Decidual antigen presenting cells (DAPCs) co-cultured with TNP-modified autologous T cells induced the development of cytotoxic anti-TNP T lymphocytes with an efficiency comparable to that of peripheral blood antigen presenting cells (PAPCs). Treatment with anti HLA-class II antibody plus complement and UV radiation resulted in substantial inhibition of the accessory cell function. The T cell mediated lysis of TNP-modified targets is restricted by the major histocompatibility complex. Our results show that HLA-class I molecules are the most prominent restriction elements. The relevance of these data to the immunological mechanisms operating at the feto-maternal interface is discussed.
D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology*; D003656:Decidua / Q000276:immunology*; D005260:Female; D006681:HLA-D Antigens / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D011247:Pregnancy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D006681:HLA-D Antigens; D014301:Trinitrobenzenes