Identification of some interleukin-1 (IL-1) sensitive organs in vivo using the induction of ornithine decarboxylase (ODC) following injection of recombinant IL-1 beta in mice.
Intraperitoneal (i.p.) and intravenous (i.v.) injection of human recombinant interleukin-1 beta (rIL-1 beta) in mice produced a 2-4 fold induction of ornithine decarboxylase (ODC) in liver and heart six hours after administration. Lymphoid organs (thymus and spleen) and brain did not respond to rIL-1 beta administration with significant increases in ODC. IL-1-induced responses in heart seemed not to be secondary to stress induced catecholamine release since the beta-adrenergic antagonist propranolol did not inhibit the induction of ODC produced by injection rIL-1 beta. Injection of 10 micrograms bacterial lipopolysaccharide (LPS), an inducer of IL-1 synthesis, exhibited a pattern of tissue responsiveness which was distinct from the responses elicited by rIL-1 beta, most notably a marked 5-fold induction of ODC in spleen. The differences in the responses of various organs to rIL-1 beta vs. LPS suggested that the in vivo effects of LPS may involve more than stimulation of the release of IL-1. The identification of heart as an IL-1 sensitive tissue merits further study to define the contribution of IL-1 to cardiac physiology and pathophysiology.
D000818:Animals; D006321:Heart / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007375:Interleukin-1 / Q000008:administration & dosage* / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D009206:Myocardium / Q000201:enzymology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010446:Peptide Fragments / Q000008:administration & dosage; D011433:Propranolol / Q000494:pharmacology; D011994:Recombinant Proteins / Q000008:administration & dosage; D012485:Salmonella typhi; D014781:Viscera / Q000187:drug effects / Q000201:enzymology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D010446:Peptide Fragments; D011994:Recombinant Proteins; D011433:Propranolol; D009955:Ornithine Decarboxylase