Myeloid progenitors in acute non-lymphocytic leukemia: prognostic value in remission.
The prognostic value of in vitro cloning of bone marrow and blood cells was tested in 32 patients with acute non-lymphocytic leukemia (ANLL) at various stages of disease. Using placental conditioned medium (PCM) as a stimulus and panoptical staining methods for the analysis of colony morphology, four different growth types could be distinguished at presentation. Poor response to colony-stimulating activity (GM-CSF) in vitro seemed to be associated with poor survival. Probably due to low patient numbers, this association could not be proven statistically. During remission a progressive decrease of morphologically normal colonies was recognized in the bone marrow and peripheral blood. Thus by in vitro cloning methods the quality of remission could be substantiated.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000188:drug therapy / Q000401:mortality / Q000473:pathology*; D008264:Macrophages / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013997:Time Factors
D003115:Colony-Stimulating Factors