Spontaneous growth of colonies with mature T lineage phenotype from T-cell-depleted bone marrow precursors in a patient with a lymphoproliferative disorder of the large granular lymphocytes.
Bone marrow cells from a patient with pancytopenia and a lymphoproliferative disorder of large granular lymphocytes (LGL) were cultured and tested for their hemopoietic colony-forming potential. Neither erythroid nor granulocyte-macrophage colony formation could be obtained from unfractionated, or LGL-depleted bone marrow cell preparations. However, a spontaneous growth of lymphoid colonies was observed after culturing LGL-depleted (T3-) bone marrow cell suspensions for 25 days. Pooled colonies expanded with recombinant interleukin-2 yielded a population composed predominantly of mature T cells (T3+, Leu 6-). These findings suggest that some (T3-) T cell precursors may mature in the bone marrow and that, in our patient, LGL may have exerted a suppressor effect on this maturational process.
D000368:Aged; D000954:Antigens, Surface / Q000032:analysis; D001854:Bone Marrow Cells; D002460:Cell Line; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000032:analysis*; D006801:Humans; D007694:Killer Cells, Natural / Q000503:physiopathology*; D008212:Lymphocyte Depletion; D008232:Lymphoproliferative Disorders / Q000503:physiopathology*; D008264:Macrophages / Q000166:cytology; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology*
D000954:Antigens, Surface