T-24.B-cell differentiation factor induces immunoglobulin secretion in human B cells without prior cell replication.
Stimulation of B lymphocytes from B-cell chronic lymphocytic leukaemia (B-CLL) with 12-0-tetradecanoylphorbol-13-acetate (TPA) has shown that these cells are capable of differentiation (Totterman, Nilsson & Sundstrom, 1980). Increases in the expression of different class II MHC antigens (Guy et al., 1983, 1986) and responsiveness to growth factors (Kabelitz et al., 1985; Suzuki, Butler & Cooper, 1985) have been studied. Supernatant from the human bladder carcinoma line T-24 contains a B-cell differentiation factor (BCDF) able to induce immunoglobulin secretion from CESS cells. We investigated the induction of proliferation and immunoglobulin secretion in human B cells by studying the effects of this factor on B-CLL cells, in both the presence and absence of TPA. We report here that this material (termed T-24.BCDF) causes immunoglobulin secretion to be initiated in these cells, and that this is not accompanied by detectable DNA synthesis. These observations were extended to normal human B cells and demonstrate that human B cells can secrete immunoglobulin in the absence of clonal expansion.
D001402:B-Lymphocytes / Q000276:immunology*; D002455:Cell Division; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D006133:Growth Substances / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D015847:Interleukin-4; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000276:immunology*; D008297:Male; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D004273:DNA, Neoplasm; D006133:Growth Substances; D007136:Immunoglobulins; D008222:Lymphokines; D015847:Interleukin-4; D013755:Tetradecanoylphorbol Acetate