title: An in-vitro study of enamel protein degradation in developing bovine enamel.
abstract: An immature enamel fraction, as far as possible without cells, was prepared from fetal bovine molars, using aqueous-density fractionation. Portions were incubated at 37 degrees C with or without protease inhibitors. Amelogenins and enamelins were then examined for their molecular weight using HPLC-gel permeation. Degradation of amelogenins occurred rapidly and appeared to be related to proteolytic activity, probably localized extra-cellularly. Enamelins remained almost stable over the time intervals used.
mesh_terms: D053523:Amelogenin; D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003746:Dental Enamel Proteins / Q000378:metabolism*; D005333:Fetus; D014083:Tooth Germ / Q000378:metabolism*
chemical_list: D053523:Amelogenin; D003746:Dental Enamel Proteins; C038456:tuftelin