Motoneuron number in the lumbar lateral motor column of larval and adult bullfrogs.
Motoneuron number in the lumbar lateral motor column of the bullfrog, Rana catesbeiana, was investigated through the course of premetamorphic development and in postmetamorphic frogs. Motoneurons were distinguished on the basis of histological characteristics into two classes, type L (less differentiated) and type M (more differentiated). The number of type L motoneurons on each side showed a precipitous decline between stages V and VI (6,300 to 2,500) and a slower rate of loss until stage XI (to 550). Type M motoneurons increased in number between stages V and VII (560 to 2,775) and declined precipitously between stages VII and VIII to a value similar to that of juvenile frogs (1,100). These changes in motoneuron number do not correspond to the formation of myotubes or to the appearance of contractile properties in hindlimb muscles. The development of myotubes in the hindlimb occurs only after total motoneuron number has declined by 35%. Similarly, hindlimb muscle contraction develops after the early decline in type L motoneuron number and is restricted to proximal thigh at the peak of type M motoneuron number. In postmetamorphic frogs, a weak (r = 0.44) but statistically significant correlation was found between type M motoneuron number and body length. In the largest frogs (greater than 15 cm body length), 1262 +/- 157 (mean +/- s.d.) motoneurons were present, whereas the smallest frogs (less than 5 cm body length) had 1099 +/- 98 motoneurons. These results are not consistent with previous findings that the variance of motoneuron number among small frogs is greater than that among larger frogs. The present results are thus inconsistent with explanations of size-related differences in motoneuron number that are based on selection of small frogs with greater number of motoneurons for survival. The increase in motoneuron number may be due to a slow addition of newly born motoneurons to the LMC or to the differentiation of existing motoneurons. The latter possibility is supported by the finding that the number of presumptive type L profiles is less in larger frogs.
D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D007814:Larva; D009046:Motor Neurons / Q000166:cytology*; D009940:Organoids / Q000648:ultrastructure; D011892:Rana catesbeiana / Q000254:growth & development*; D013116:Spinal Cord / Q000254:growth & development*