Human monoclonal antibodies produced from L-leucine methyl ester-treated and in vitro immunized peripheral blood lymphocytes.
Primary in vitro immunization of peripheral blood lymphocytes has been studied for the production of human monoclonal antibodies. Depletion of a lysosome-rich cell population, containing large granular lymphocytes, monocytes, and a subset of T suppressor cells, was shown to be crucial before the cells could be immunized in vitro. The in vitro immunized lymphocytes were used to produce human x human and human x mouse hybridomas secreting monoclonal antibodies specific for digoxin, hemocyanin, or a recombinant fragment of gp120 of human immunodeficiency virus.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000943:Antigens, Differentiation / Q000032:analysis; D006801:Humans; D007114:Immunization; D066298:In Vitro Techniques; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; C030365:leucine methyl ester; D007930:Leucine