B-lymphocyte colony formation in chronic lymphocytic leukemia.
A semisolid culture system for B-cell colony formation is described. The system includes pretreatment of B-cells by neuraminidase-galactose oxidase and help of mitomycin-treated T-cells. With this assay system, colony-forming B-cell precursors were detected in all eight patients we studied with B-cell chronic lymphocytic leukemia. These patients' own T-cell helper effect was less than that of normal T-cells.
D000368:Aged; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged