Delayed-type hypersensitivity to transplantation antigens in mice and its investigation under conditions of allograft and xenograft tolerance.
Methods of induction of delayed-type hypersensitivity (DTH) to transplantation alloantigens and xenoantigens in mice were developed. They include the s.c. sensitization of mice with spleen cells (5 X 10(6) xenogeneic cells in CFA or 1 X 10(7) allogeneic cells) followed 5 days later by testing of the reactions. Reactions induced to alloantigens and to xenoantigens were the DTH reactions: they reached a maximum 24 h after testing, were mediated by T lymphocytes, were not transferred to intact recipients with serum from sensitized donors. DTH reactivity to transplantation antigens was employed to assess the immune reactivity of tolerant animals. It is shown that the tolerance induced in mice by our method leads to prolonged survival of allogeneic and xenogeneic neonatal heart grafts and is accompanied with specific DTH nonreactivity. The utilization of DTH to transplantation antigens as a test for tolerance establishment and as a model for studying its mechanisms is discussed.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007108:Immune Tolerance; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology; D013934:Thymectomy; D014181:Transplantation Immunology; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous
D003520:Cyclophosphamide