title: Natural and recombinant human interleukin 1-beta is cytotoxic for human melanoma cells.
abstract: Human peripheral blood monocytes stimulated with LPS were found to release an activity that was cytotoxic for the A375 melanoma. Biochemical and immunological characterization of the activity indicated that IL 1-beta was the cytotoxic agent. Human recombinant IL 1-beta, purified to homogeneity, was directly cytotoxic for A375. Tumor necrosis factor, also released by activated monocytes, was not cytotoxic for the A375 melanoma.
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000502:physiology*; D002460:Cell Line; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006023:Glycoproteins / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000302:isolation & purification / Q000502:physiology*; D008545:Melanoma / Q000276:immunology*; D009000:Monocytes / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D006023:Glycoproteins; D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha