Two different 7-ethoxycoumarin O-deethylase activities in mice after pretreatment with sulmazole and cobaltous chloride.
Pretreatment of male C57BL/6J Han mice with 200 mg of sulmazole (AR-L 115 BS) daily for 2 days results in an up to 4fold increase of the 7-ethoxycoumarin O-deethylase. The resulting effect was compared to that after a pretreatment with cobaltous chloride which also leads to a 4fold increase. Enzyme kinetic parameters of the 7-ethoxycoumarin O-deethylase are different for the two inducers in respect to the affinity for the substrate, but the inhibitions by metyrapone and alpha-naphthoflavone are similar. Comparison of the microsomal protein patterns after partial purification of microsomes revealed totally different patterns after sulmazole and cobaltous chloride. Sulmazole produces a 54 kDa protein band and cobaltous chloride a band at 48.5 kDa. Therefore it is concluded that in mice at least two 7-ethoxycoumarin O-deethylase activities can be induced. The electrophoretic pattern after sulmazole is different from those after 3-methylcholanthrene and isosafrole. This was also proven by enzyme kinetic investigations with ethoxyresorufin as substrate.
D015660:7-Alkoxycoumarin O-Dealkylase; D000818:Animals; D003035:Cobalt / Q000494:pharmacology*; D003640:Dealkylation; D004591:Electrophoresis, Polyacrylamide Gel; D007093:Imidazoles / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000201:enzymology; D010105:Oxygenases / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D014468:Umbelliferones / Q000378:metabolism
D007093:Imidazoles; D014468:Umbelliferones; D012967:Sodium Dodecyl Sulfate; D003035:Cobalt; D010105:Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; C018021:cobaltous chloride; C029758:sulmazole