Idiotype vaccination against murine B cell lymphoma. Inhibition of tumor immunity by free idiotype protein.
A murine B cell lymphoma (38C13) was used as a model to study the induction of idiotype (Id)-specific tumor immunity. Immunization of syngeneic mice with Id protein derived from the tumor resulted in the production of anti-Id antibodies by the host and in the induction of a state of resistance to tumor growth. Tumor immunity could be established only if the Id protein was conjugated to a strongly immunogenic carrier protein such as keyhole limpet hemocyanin or thyroglobulin, and if the conjugate was administered at least 1 week prior to tumor challenge. Free Id protein, such as that present in tumor bearing animals, was found to inhibit tumor immunity in a dose-dependent manner. Although tumor immunity could be induced in animals with pre-existent serum Id protein, the expression of the immune state was inhibited by the presence of the soluble protein.
D000276:Adjuvants, Immunologic / Q000276:immunology; D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D007130:Immunoglobulin Idiotypes / Q000008:administration & dosage / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D014611:Vaccination
D000276:Adjuvants, Immunologic; D000912:Antibodies, Neoplasm; D007130:Immunoglobulin Idiotypes