Correlation between adriamycin-induced augmentation of interleukin 2 production and of cell-mediated cytotoxicity in mice.
Based on the observation that spleen cells from Adriamycin-treated mice could develop augmented levels of cytotoxic T-lymphocyte activity in response to heat-treated and/or X-irradiated alloantigens, it was postulated that modulations in soluble mediators could be involved in this phenomenon. In fact, in this study Adriamycin-induced increases in the levels of prostaglandin E2 and interleukin 2 activity have been observed with isolated cells. The "interleukin 2-like" activity was indistinguishable from that of partially purified interleukin 2 in terms of ability to restore responsiveness to experimentally inhibited primary alloantigen response cultures and to maintain long-term cultures of activated T-cells. Furthermore this latter activity was completely ablated by antiinterleukin 2 monoclonal antibody. While the modification in prostaglandin E2 production did not appear to play a role in determining augmentation of cytotoxic T-cell activity, the modification in interleukin 2 production was consistent with the possibility that this is a primary mechanism of Adriamycin-induced augmented cell-mediated cytotoxicity.
D000818:Animals; D003470:Culture Media; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D015232:Dinoprostone; D004317:Doxorubicin / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011458:Prostaglandins E / Q000096:biosynthesis; D013154:Spleen / Q000276:immunology
D003470:Culture Media; D007375:Interleukin-1; D007376:Interleukin-2; D011458:Prostaglandins E; D004317:Doxorubicin; D015232:Dinoprostone