The tumor promoter tetradecanoylphorbol-13-acetate elicits the redistribution of heavy metals in subcellular fractions of rabbit thymocytes as measured by plasma emission spectroscopy.
Prolonged (90 min) incubation with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) causes no significant changes in the elemental composition of whole rabbit thymocytes (25 elements included). The only exception is the amount of magnesium, where a significant increase can be detected. However TPA preincubation elicits the redistribution of heavy metals (mainly Zn and to a much lesser extent Fe and Cu) from the nuclear and large granular (mitochondrial-lysosomal) fraction to the cytosol and microsomes. A significant translocation of calcium and phosphorus from the large granular fraction mainly to the nucleus can also be observed. The TPA-induced heavy metal depletion of the nucleus might play a role in the antiproliferative activity of TPA, the arrest of G1----S phase transition. On the other hand the increased amount of heavy metals in the cytosol might be linked to the TPA-induced block of intercellular communication and the desensitization of TPA-treated cells towards extracellular calcium which are well-known properties of neoplastic cells.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D003600:Cytosol / Q000032:analysis; D008670:Metals / Q000032:analysis*; D008861:Microsomes / Q000032:analysis; D011817:Rabbits; D013057:Spectrum Analysis; D013601:T-Lymphocytes / Q000032:analysis / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D008670:Metals; D013755:Tetradecanoylphorbol Acetate