ELISA tests for dapsone and pyrimethamine and their application in a malaria chemoprophylaxis programme.
Enzyme-linked immunosorbent asays (ELISAs) are described for determining levels of dapsone and pyrimethamine in urine. Both assays have a sensitivity of about 20 mug/l and are reproducible, but each produces some false positives. The problem of false positive reactions was partially obviated by requiring positive results in both assays. In a pilot study involving 50 children aged 3 months to 4 years who were given a single dose of Maloprim (pyrimethamine + dapsone), 75% were positive for dapsone 7 days after administration of the drug, while 25% were still positive 15 days after its administration. The corresponding proportions for pyrimethamine were 73% and 30%, respectively. Comparison of the results obtained in a larger chemoprophylaxis trial with those from the pilot study indicated that the assays described could be used to investigate whether antimalarials had been taken.
D002675:Child, Preschool; D003622:Dapsone / Q000627:therapeutic use* / Q000652:urine; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D005714:Gambia; D006801:Humans; D007223:Infant; D008288:Malaria / Q000517:prevention & control*; D011739:Pyrimethamine / Q000627:therapeutic use* / Q000652:urine
D004338:Drug Combinations; D003622:Dapsone; D011739:Pyrimethamine