Identification of a flunixin metabolite in the horse by gas chromatography-mass spectrometry.
The main metabolite of flunixin, a hydroxylated product, has been identified by gas chromatography-mass spectrometry and 1H NMR spectroscopy in equine urine and plasma. The method also permits the qualitative monitoring of the urinary elimination of the drug and its metabolite. The two products are detected up to 175 and 54 h, respectively, after a single intravenous administration at the dose of 1 mg/kg. Simultaneous detection of the two compounds increases the reliability of anti-doping control analysis.
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000097:blood / Q000652:urine; D001711:Biotransformation; D003002:Clonixin / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009539:Nicotinic Acids / Q000032:analysis*; D013055:Spectrophotometry, Infrared
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009539:Nicotinic Acids; C014557:flunixin; D003002:Clonixin