Automated capillary gas chromatographic assay using nitrogen-phosphorus ionization detection for the determination of bepridil in human plasma.
A highly sensitive and specific capillary gas chromatographic method for the determination of the antianginal drug bepridil in plasma is described. The capillary gas chromatograph and nitrogen-selective detector combination provides excellent sensitivity for clinical samples. The lowest concentration of bepridil which can be measured accurately and precisely in a 1-ml plasma sample is 1 ng/ml. Standard curves are linear over the concentration range 1-60 ng/ml. Accuracy and precision of the assay, expressed as relative deviation from the true value and relative standard deviation (inter-run) are less than 15% at all concentrations in the linear range. No interfering peaks are observed. Using an automatic injector and a laboratory computer system, sixty samples can be analyzed routinely in one day. The present assay has been successfully cross-validated with a published high-performance liquid chromatographic assay.
D015764:Bepridil; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D011759:Pyrrolidines / Q000097:blood*
D007202:Indicators and Reagents; D011759:Pyrrolidines; D015764:Bepridil