Relative proportions of mitotic T and B cells in PHA-stimulated lymphocyte cultures.
Peripheral blood lymphocytes from six healthy adults were stimulated with phytohemagglutinin (PHA) and cultured for 3 days. The relative proportions of mitotic lymphocyte subsets were determined by a method that allows both cytogenetic and immunologic characterization of a mitotic cell. The results directly indicated that not only T cells but also B cells undergo mitosis in PHA-stimulated lymphocyte cultures. The frequency of the mitotic B cells varied between 6% and 20%.
D000328:Adult; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology*; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation; D008875:Middle Aged; D008938:Mitosis; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology*
D000911:Antibodies, Monoclonal; D010835:Phytohemagglutinins