Purification and characterization of human granulocyte colony-stimulating factor (G-CSF).
A colony-stimulating factor (CSF) has been purified to homogeneity from the serum-free medium conditioned by one of the human CSF-producing tumor cell lines, CHU-2. The molecule was a hydrophobic glycoprotein (mol. wt 19,000, pI = 6.1 as asialo form) with possible O-linked glycosides. Amino acid sequence determination of the molecule gave a single NH2-terminal sequence which had no homology to the corresponding sequence of the other CSFs previously reported. The biological activity was apparently specific for a neutrophilic granulocyte-lineage of both human and mouse bone marrow cells with a specific activity of 2.7 X 10(8) colonies/10(5) non-adherent human bone marrow cells/mg protein. The purified CSF can be regarded as a G-CSF of human origin and will become a useful material for investigation of regulatory mechanisms of human granulopoiesis.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003115:Colony-Stimulating Factors / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight
D000596:Amino Acids; D003115:Colony-Stimulating Factors; D003470:Culture Media