Comparison of efficiency of complements from various species for T-cell depletion from Cercopithecus aethiops bone marrow with Campath-1 MoAb in vitro.
Mature T-cells were removed from Cercopithecus aethiops monkey bone marrow with Campath-1 MoAb plus complement from various species (man, rabbit and monkey). The T-cell depletion was more effective and stable with rabbit or human complement than with autologous (monkey) complement. The most complete and effective lytic function to T-cells was found in the case of rabbit complement. Rabbit complement can be used successfully to deplete bone marrow T-cells of C. aethiops with Campath-1 in vitro.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001854:Bone Marrow Cells; D002521:Cercopithecus / Q000276:immunology; D002522:Chlorocebus aethiops / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D006801:Humans; D008212:Lymphocyte Depletion; D011817:Rabbits / Q000276:immunology; D051381:Rats; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003165:Complement System Proteins