Purification of the enhancing factor from mouse intestines.
A unique polypeptide, called enhancing factor (EF), which enhances the binding of labeled epidermal growth factor (EGF) to cells, has been isolated. It has been purified to homogeneity from the acid-soluble proteins of mouse intestines. Earlier, EF was partially purified by two cycles of gel-permeation chromatography on Bio-Gel columns. We now report the final purification of EF on high-performance liquid chromatography (HPLC), using a reverse-phase column (mu Bondapak C18). The purity of the protein was confirmed when a single peak was obtained in HPLC. Also, a single protein band was obtained in SDS-PAGE. Purified EF has the same properties in vitro as those reported earlier for partially purified EF.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004815:Epidermal Growth Factor / Q000378:metabolism*; D054501:Group II Phospholipases A2; D007422:Intestines / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D010741:Phospholipases A
D000596:Amino Acids; D010455:Peptides; D004815:Epidermal Growth Factor; D010741:Phospholipases A; D054501:Group II Phospholipases A2; C516288:Pla2g2a protein, mouse