The effect of Gla-containing proteins on the precipitation of insoluble salts.
The precipitation of insoluble salts containing divalent metal ions is inhibited by Gla-containing proteins of various origin. In this paper we demonstrate that: Gla-residues are required for the inhibitory activity; the inhibition is effected by a protein which in vivo is bound to calcified tissue (osteocalcin) as well as by proteins occurring in blood plasma (factor X) and urine (the urinary Gla-protein); The inhibitor concentration required for 50% precipitation-inhibition varied slightly from one salt to the other, but no marked differences were observed between the effects of the various Gla-containing proteins used; Precipitation-inhibition occurred in all phosphates (Be, Ca, Mn and Zn) and in all calcium salts (phosphate, oxalate and carbonate) tested.
D015055:1-Carboxyglutamic Acid / Q000502:physiology*; D002130:Calcium Phosphates / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D011232:Chemical Precipitation; D005170:Factor X / Q000378:metabolism; D015675:Osteocalcin; D010710:Phosphates / Q000378:metabolism; D012492:Salts / Q000378:metabolism*; D012995:Solubility; D014812:Vitamin K / Q000502:physiology
D002130:Calcium Phosphates; D002135:Calcium-Binding Proteins; D002413:Cations, Divalent; D010710:Phosphates; D012492:Salts; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D015675:Osteocalcin; D014812:Vitamin K; D015055:1-Carboxyglutamic Acid; C485838:calcium phosphate, monobasic, anhydrous; D005170:Factor X; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous