[Analysis of HLA-class II antigen mRNAs on T lymphoblasts].
The expression of human class II antigens on T lineage cells was analysed at the mRNA and the protein levels. Alpha- and beta-chains of class II antigens were identified on phytoheamagglutinin (PHA)-activated T lymphoblasts by two-dimensional polyacrylamide gel electrophoresis analysis. It was, however, not clear whether their spots represent HLA-DR, DQ or -DP antigen. Northern blot analysis using cDNA probes for alpha- and beta-chains of DR, DQ, and DP antigens revealed that mRNAs for all the class II antigen components were detected in PHA-activated T lymphoblasts. Among six T lymphoblastoid cell lines studied (Molt 4, RPMI 8402, CCRF-CEM, TALL 1, HSB 2 and MT-2), only MT-2, a human T cell leukemia virus-1 producing line, expressed class II antigen molecules and their mRNAs. The rest of the lines did not express class II antigens either at the protein level or at the mRNA level. However, Southern hybridization showed that these cell lines retained DQ beta-chain gene at the DNA level.
D000911:Antibodies, Monoclonal / Q000276:immunology; D004247:DNA / Q000032:analysis; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis*; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D004247:DNA