Stimulation of collagen and glycosaminoglycan production by phenytoin 5,5-diphenylhydantoin in monolayer cultures of mesenchymal cells derived from embryonic chick sternae.
Cultures grown with or without phenytoin (PHT) at a concentration of 5 micrograms/ml from the fifth to the eighth day after plating were labelled with [14C]-proline (0.2 microCi/ml) from the sixth to eighth day. Collagenase digestion indicated that collagen content increased approx. 2-fold after PHT exposure. Increases in sulphated glycosaminoglycan product in response to PHT were approx. 1.5-fold; PHT also stimulated protein production. Both actinomycin D and cycloheximide blocked incorporation of [3H]-leucine, [3H]-proline, and H2(35)SO4 approx. 90 per cent with or without PHT. Continuous sucrose density-gradient fractionation indicated that PHT produced quantitative but not qualitative changes in cellular RNA.
D000818:Animals; D001842:Bone and Bones / Q000196:embryology*; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D010672:Phenytoin / Q000494:pharmacology*; D013249:Sternum; D013395:Sucrose / Q000378:metabolism
D006025:Glycosaminoglycans; D013395:Sucrose; D010672:Phenytoin; D003094:Collagen