Identification of inhibitory growth factors from a N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide-induced murine bladder cancer cell line.
The N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT)-induced murine bladder carcinoma cell line MBT-683 contains growth-stimulating (TGF) and growth-inhibiting factors (GI). These activities coeluted on BioGel P-30 between molecular weights 6,000 and 29,000 daltons. They can be separated by high performance liquid chromatography. Both activities were destroyed by trypsin and dithiothreitol. However, they were resistant to acid and heat. Pool 1 (TGF) stimulated the growth of normal rat kidney cells, whereas pool 2 (GI) inhibited the colony formation of the cell lines MBT-683 and RBTCC-8.
D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000032:analysis / Q000139:chemically induced*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005200:FANFT / Q000633:toxicity*; D006133:Growth Substances / Q000032:analysis*; D051379:Mice; D010455:Peptides / Q000032:analysis*; D051381:Rats; D013844:Thiazoles / Q000633:toxicity*; D015291:Transforming Growth Factors; D001749:Urinary Bladder Neoplasms / Q000032:analysis / Q000139:chemically induced*
D006133:Growth Substances; D010455:Peptides; D013844:Thiazoles; D015291:Transforming Growth Factors; D005200:FANFT