A new micromethod for determination of interleukin-1 production from frozen human blood mononuclear cells.
A microtechnique is described for inducing IL-1 activity in vitro from frozen human blood mononuclear cells (BMNC) using the lymphocyte-activating factor (LAF) method. When compared to the conventional culture system, this micromethod offers several advantages: the complete assay requires a total of 1.0 X 10(5) mononuclear cells which is easily obtained from 1 ml of whole blood; the method of isolation and freezing of cells preserves their viability and ability to produce IL-1 for several weeks; when compared to the conventional test using fresh BMNC, frozen cells produce the same amount of IL-1. The microtechnique described here is highly reproducible and provides a useful tool for evaluating the production of IL-1 from BMNC of human subjects in clinical studies including those with immunodeficiencies, malnutrition and malignant diseases.
D002452:Cell Count; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008722:Methods; D008832:Microchemistry; D010835:Phytohemagglutinins / Q000494:pharmacology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins