Identification of leukemia cell-derived inhibitory activity (LIA) in conditioned media from human myeloid leukemic cell line ML-2.
Conditioned media from the human myeloid leukemic cell line ML-2 contain a factor that inhibits the entry of normal CFU-GM into S phase of mitotic cycle as measured by the 3H-TdR suicide technique. This factor was detected in conditioned media prepared by incubating 5 X 10(6) ML-2 cells/ml or 1 X 10(6) ML-2 cells/ml in serum-free RPMI for 5 or 24 hours respectively, and was isolated by ultrafiltration through an XM 300 Diaflo membrane followed by chromatography on Sepharose 6 B. Ferritin, prepared from human placenta, had the same inhibitory effect on CFU-GM. Antibodies against human placental ferritin completely inactivated the inhibitory effect of both human placental ferritin and the factor released from ML-2 cells. The inhibitory activity produced by the cell-line ML-2 was considered as LIA (leukemia cell-derived inhibitory activity) earlier found in HL-60 cell line and AML and CML cells.
D000818:Animals; D001853:Bone Marrow; D002460:Cell Line; D002850:Chromatography, Gel; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000037:antagonists & inhibitors*; D003470:Culture Media / Q000032:analysis*; D005293:Ferritins / Q000494:pharmacology; D006098:Granulocytes; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells; D006801:Humans; D007525:Isoelectric Focusing; D007951:Leukemia, Myeloid; D008264:Macrophages; D008297:Male; D051379:Mice; D010920:Placenta
D003115:Colony-Stimulating Factors; D003470:Culture Media; C020391:acidic isoferritin; D005293:Ferritins