title: Immunological and chemical analysis of P6, the carboxyl-terminal fragment of HIV P15.
abstract: The first open reading frame of the HIV genome has been identified as the gag gene. The proteins encoded by this gene are p17 as the amino-terminal protein, p24 as the middle peptide, and p15 as the carboxyl-terminal end. A monoclonal antibody recognizing an antigenic determinant on a fragment of p15 has been developed and designated M35/2F8. This monoclonal has been instrumental in radiosequencing the carboxyl-terminal product of p15, p6, and in determining the cleavage site between this protein and the amino-terminal product, p7. By immunoaffinity chromatography it was also possible to purify p6 from HIV lysates and all p6 containing polyproteins from HIV-infected cells. These results gave more insight into the composition and processing of the HIV gag gene.
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002846:Chromatography, Affinity; D015683:Gene Products, gag; D006678:HIV / Q000032:analysis* / Q000276:immunology; D015483:HIV Antibodies; D007122:Immunoelectrophoresis; D010446:Peptide Fragments / Q000276:immunology; D011499:Protein Processing, Post-Translational; D012191:Retroviridae Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D015682:Retroviridae Proteins, Oncogenic
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015683:Gene Products, gag; D015483:HIV Antibodies; D010446:Peptide Fragments; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; C039467:virus structural protein p15