Accessory cell competence of ovine oligodendrocytes in mitogenic activation of human peripheral T cells.
The multiple sclerosis (MS) plaque is characterized by mononuclear inflammatory cell infiltration, demyelination, loss of oligodendrocytes (OGC), and proliferation of astrocytes. Although antigen-specific, Ia-dependent cellular immune mechanisms have been sought in plaque pathogenesis, Ia-independent T cell activation has not been actively investigated. We examined a potential role of OGC in accessory cell-dependent T cell mitogenesis with the anti-T3 monoclonal antibody OKT3. OGC isolated from ovine white matter on sucrose density gradients were uniformly negative for esterase activity, unlike ovine monocytes. Purified human T cells did not exhibit significant proliferation in 3-day cultures with OKT3, autologous peripheral blood adherent cells (PBAC), or ovine OGC. When T cells were cultured with either PABC or OGC in the presence of OKT3, brisk mitogenesis was observed. Thus, OGC have the capacity to function as accessory cells in the mitogen-induced proliferation of T cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001667:Binding, Competitive; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D008213:Lymphocyte Activation; D009103:Multiple Sclerosis / Q000276:immunology; D009457:Neuroglia / Q000276:immunology*; D009836:Oligodendroglia / Q000276:immunology*; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G