Immunohistochemical demonstration of various antigens in tissues embedded in plastic.
In the course of a chronic experimental study of polyarthritis in rats, we have established a plastic embedding technique which allows the processing not only of soft tissues but also of tissues such as cartilage, intraarticular ligaments, and even undecalcified whole rat joints and provides broad survival of enzymes and antigens. Preservation of morphological details and the precision of antigen localization are superior to those seen in cryostat and paraffin sections. The method seems to have much diagnostic and investigative value, especially in arthritis research.
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D005269:Femur / Q000032:analysis / Q000166:cytology; D007106:Immune Sera; D007150:Immunohistochemistry / Q000379:methods; D008198:Lymph Nodes / Q000032:analysis / Q000166:cytology; D008297:Male; D010969:Plastics; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000032:analysis / Q000166:cytology; D014021:Tissue Preservation / Q000379:methods*
D000911:Antibodies, Monoclonal; D000941:Antigens; D007106:Immune Sera; D010969:Plastics