An improved method for recovery of secretory immunoglobulins and lymphocytes from the nasal mucosa.
The difficulty of obtaining adequate specimens for assay has severely restricted in vivo investigations of local immune responses in humans. Washing the posterior nasopharynx for an extended period using chilled saline to stimulate serous secretions has improved yields of both secretory immunoglobulins and functionally competent immunocytes. The proportions of T- and B-cells found in such washings appear to differ from those found in blood.
D000469:Alkaline Phosphatase; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000302:isolation & purification*; D008214:Lymphocytes / Q000145:classification* / Q000201:enzymology; D008297:Male; D009297:Nasal Mucosa / Q000166:cytology / Q000276:immunology*; D011506:Proteins / Q000032:analysis; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology; D007507:Therapeutic Irrigation / Q000379:methods*
D000954:Antigens, Surface; D007071:Immunoglobulin A, Secretory; D011506:Proteins; D000469:Alkaline Phosphatase