title: In vitro effects of serotonin and prostaglandins on release of eggs from the ovary of the scallop, Patinopecten yessoensis.
abstract: When ovarian pieces of the scallop, Patinopecten yessoensis, were incubated in media containing serotonin (5-HT), the number of released eggs increased significantly. These eggs developed normally after fertilization. The threshold of the 5-HT effect reached a maximum at a concentration of 10(-6) M, and decreased sharply with increasing concentrations. Methysergide, a 5-HT antagonist, completely inhibited the 5-HT-induced egg release. Addition of aspirin or indomethacin to the incubation media also inhibited the 5-HT effect. However, neither aspirin nor indomethacin inhibited the 5-HT-induced egg release when 10(-6) M prostaglandin E2 (PGE2) was also present. PGE2 did not induce egg release, but enhanced the 5-HT effect. In contrast to PGE2, PGF2 alpha significantly inhibited the 5-HT effect. These results show that 5-HT induces spawning of female P. yessoensis via specific 5-HT receptors in the ovary, and that the effect of 5-HT may be regulated by PGE2 (progressive) and PGF2 alpha (suppressive).
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D015237:Dinoprost; D015232:Dinoprostone; D004298:Dopamine / Q000494:pharmacology; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008784:Methysergide / Q000494:pharmacology; D008974:Mollusca; D010053:Ovary / Q000187:drug effects / Q000502:physiology; D010063:Ovum / Q000187:drug effects / Q000502:physiology*; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology*; D013469:Sulpiride / Q000494:pharmacology
chemical_list: D011458:Prostaglandins E; D011460:Prostaglandins F; D012701:Serotonin; D013469:Sulpiride; D015237:Dinoprost; D015232:Dinoprostone; D001241:Aspirin; D004298:Dopamine; D007213:Indomethacin; D008784:Methysergide