Loss of inducible photorepair in a frog cell line hypersensitive to solar UV light.
The induction of enzymatic photorepair (EPR) in ICR 2A frog cells and a derived mutant cell line DRP36 hypersensitive to solar UV was studied. Using clonogenic assays, when induced wild-type cells demonstrated an 8-fold increase of EPR the mutant cells displayed a near-background level of inducible EPR. The constitutive EPR in mutant cells, however, was the same as in wild-type cells. A mixed culture of ICR 2A and DRP36 cells showed an intermediate inducible EPR depending upon the cell ratio. Inducible EPR was also detected at the DNA level in wild-type cells, but not in mutant cells.
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects*; D008027:Light; D008970:Molecular Weight; D009154:Mutation; D011894:Rana pipiens; D013472:Sunlight; D014466:Ultraviolet Rays
D004247:DNA