Amelioration of murine lupus nephritis by dimethylsulfoxide.
Dimethylsulfoxide was given to NZB/W F1 female mice from age 10 weeks to see if proteinuria and glomerular injury could be reduced. Twenty mice were randomly assigned to saline or DMSO treatment groups and the following studies were done: urine protein determination, serum concentrations of creatinine, IgG, C3, and albumin; and ANA titers. Kidney tissue were studied by light, immunofluorescent and electron microscopy. DMSO-treated mice had significant reductions in protein excretion at 5 and 6.5 months of age; in urine protein/creatinine ratio at 6.5, 7, and; 7.5 months; in serum C3 at 7.5 months; and in serum creatinine concentration. There were no significant differences among serum IgG, nor among the ANA titers. Histopathologic studies revealed nearly normal kidneys in 5/6 DMSO-treated mice whereas 4/8 controls had severe mixed membranous and membranoproliferative glomerulonephritis. Ultrastructural studies revealed mesangial, subendothelial, and subepithelial deposits and membranous transformation of the glomerular capillary wall. DMSO therefore appears capable of ameliorating glomerular injury in NZB/W F1 mice.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis; D001327:Autoimmune Diseases / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D003176:Complement C3 / Q000032:analysis; D003404:Creatinine / Q000652:urine; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use*; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis; D007668:Kidney / Q000473:pathology; D008181:Lupus Nephritis / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D051379:Mice; D008814:Mice, Inbred NZB; D011507:Proteinuria / Q000209:etiology / Q000652:urine
D000974:Antibodies, Antinuclear; D003176:Complement C3; D007074:Immunoglobulin G; D003404:Creatinine; D004121:Dimethyl Sulfoxide