Uromodulin. An immunosuppressive 85-kilodalton glycoprotein isolated from human pregnancy urine is a high affinity ligand for recombinant interleukin 1 alpha.
Uromodulin is an 85-kDa immunosuppressive glycoprotein originally isolated from human pregnancy urine. It exhibits immunosuppressive activity in vitro at concentrations between 10(-9) and 10(-11) M. Recent data demonstrate that uromodulin is able to specifically inhibit in vitro assays dependent upon interleukin 1 (IL-1). We now present evidence that uromodulin is a high affinity ligand for recombinant murine IL-1 alpha. Since uromodulin has been purified to homogeneity, this should allow extensive further characterization of the mechanism of action of both uromodulin and IL-1.
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007165:Immunosuppression Therapy; D007375:Interleukin-1; D007700:Kinetics; D008024:Ligands; D051379:Mice; D009088:Mucoproteins / Q000378:metabolism / Q000652:urine; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism / Q000652:urine*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D058949:Uromodulin
D007375:Interleukin-1; D008024:Ligands; D009088:Mucoproteins; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C548612:UMOD protein, human; C548613:Umod protein, mouse; D058949:Uromodulin