A simple and rapid chemical method for the determination of cephalosporins.
A simple and rapid chemical assay for cephalosporins is described. It is a simple modification of the colorimetric determination of penicillins in which the narrow spectrum beta-lactamase (penicillinase) is replaced by a broad spectrum beta-lactamase (cephalosporinase) produced by Enterobacter cloacae. The method can be used for assay of fermentation broths as well as for pure cephalosporins.
D002510:Cephalosporinase / Q000378:metabolism; D002511:Cephalosporins / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D007202:Indicators and Reagents; D007700:Kinetics; D008982:Molybdenum; D010405:Penicillinase
D002511:Cephalosporins; D007202:Indicators and Reagents; C017541:molybdenum blue; D008982:Molybdenum; D002510:Cephalosporinase; D010405:Penicillinase