title: Response to ConA and PHA of host and donor thymic cells in radiation bone marrow chimeras.
abstract: The response of thymic cells to ConA and PHA was followed during 49 days in 9 Gy-irradiated C3H mice reconstituted with (C3H X AKR)F1 BM. Thymic suspension were fractionated firstly according to their capacity to bind PNA, and secondly by their Thy-1 surface antigen: Thy-1.1 antiserum was used either to lyse doner-derived cells or to separate them from host cells by panning. From days 14 to 25, when the donor-derived elements expand rapidly, the PNA- fraction remains stable and equal to 6%, suggesting that PNA+ and PNA- cell populations develop independently of each other. On the contrary, the PNA- fraction which initially represents 6% of the surviving cells rises up to 12% after day 20 and remains at this level in the long-lived host population until the end of the experiment (49 days). The response to ConA returns to normal as early as 15 days after X-rays in the PNA- fraction, whereas the response to PHA is still impaired at the end of the experiment. In both cases host cells express a higher level of responsiveness to mitogens than donor cells.
mesh_terms: D000371:Agglutination / Q000187:drug effects; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D000954:Antigens, Surface / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology / Q000528:radiation effects*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D019887:Peanut Agglutinin; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011828:Radiation Chimera / Q000187:drug effects; D013601:T-Lymphocytes / Q000145:classification; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects
chemical_list: D000954:Antigens, Surface; D037102:Lectins; D019887:Peanut Agglutinin; D010835:Phytohemagglutinins; D003208:Concanavalin A