Prostaglandin E2 inhibits the generation of phytohemagglutinin-activated killer activity in human peripheral blood lymphocytes.
The effect of prostaglandin E2 (PGE2) on the generation of phytohemagglutinin (PHA)-activated killer (PAK) activity in human peripheral blood lymphocytes (PBL) was examined. PBL from normal subjects, when incubated in vitro with PHA, lysed natural killer-resistant Daudi tumor cells during a short-term 51Cr-release assay. The generation of such PAK activity was significantly inhibited in a dose-dependent manner by PGE2 added only at the initiation of cultures. However, this inhibition of PAK activity by PGE2 was reversed by the simultaneous addition of exogenous interleukin-2 (IL-2). These findings suggest the possibility that PGE2 regulates PAK activity in PBL by inhibiting the production of IL-2 by activated T cells.
D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D015232:Dinoprostone; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology*
D007376:Interleukin-2; D010835:Phytohemagglutinins; D011458:Prostaglandins E; D015232:Dinoprostone