Normal human serum-stimulating activity on granulocyte-macrophage colony formation in vitro.
The action of human serum on granulocyte-macrophage progenitors (CFC-gm) from normal human bone marrow has been studied. When human serum was added to a culture of nonadherent bone marrow cells, no colony formation was observed in the absence of exogenous colony-stimulating factor (CSF); however, the number of colonies increased with the addition of exogenous CSF. When serum was added to a culture of unseparated bone marrow buffy coat cells, colony formation appeared even in the absence of exogenous CSF. These results show a synergic effect of serum and exogenous CSF and suggest that adherent cells, when stimulated by a serum component, secrete endogenous CSF.
D001798:Blood Proteins / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects* / Q000276:immunology; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008747:Methylcellulose; D010924:Placental Extracts / Q000494:pharmacology; D013234:Stem Cells / Q000502:physiology*
D001798:Blood Proteins; D003115:Colony-Stimulating Factors; D003470:Culture Media; D010924:Placental Extracts; D008747:Methylcellulose