Anti-idiotypic sera against monoclonal anti-progesterone antibodies: production in rabbits and rats and characterization of specificity.
Antisera were raised in rabbits and rats against three mouse monoclonal anti-progesterone IgG1 antibodies. Anti-idiotypic antibodies were isolated from rabbit sera by successive passage over immunoadsorbent columns of normal mouse Ig and the specific immunizing monoclonal, with elution from the latter. Radioimmunoassays for anti-idiotype and free idiotype were established, enabling detection of idiotype in sera of mice immunized with progesterone-BSA conjugate. Binding of rabbit anti-idiotype to anti-progesterone monoclonals was partially inhibitable by free progesterone-hemisuccinate or progesterone-ovalbumin conjugate. While showing considerable specificity for their respective inducing monoclonals, the anti-idiotypes also cross-reacted in varying degrees with the other anti-progesterone monoclonals, demonstrating the presence of IdI and IdX determinants. The patterns of cross-reactivity showed some correlation with the relative isoelectric points and combining-site specificities of the anti-progesterones. The specificities of rat and rabbit anti-idiotypes were similar, but not identical.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D003429:Cross Reactions; D005260:Female; D007130:Immunoglobulin Idiotypes / Q000032:analysis / Q000276:immunology*; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D011374:Progesterone / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D011374:Progesterone