Directed fusion in hybridoma production.
We have attempted to increase the frequency of azobenzene arsonate-specific hybrids by bridging specific B cells to the myeloma partner cells prior to polyethylene glycol-induced fusion. Bridging was accomplished by prelabeling the B cells with avidin-labeled antigen and incubating them with myeloma cells that had been modified directly with biotin. We have tested this system of hybridization with B cells from normal mice, and mice undergoing both primary and secondary responses. We found that the method is fruitful for IgG-secreting hybridomas of moderately high affinity.
D000915:Antibody Affinity; D000918:Antibody Specificity; D001360:Avidin; D001402:B-Lymphocytes / Q000276:immunology; D001710:Biotin; D002459:Cell Fusion; D004306:Dose-Response Relationship, Immunologic; D006825:Hybridomas; D013154:Spleen / Q000166:cytology; D010132:p-Azobenzenearsonate / Q000276:immunology
D001360:Avidin; D001710:Biotin; D010132:p-Azobenzenearsonate