Rapid method for purification of human T lymphocytes for further functional studies.
We have evaluated a commercially available reagent, T Lympho-kwik, for ability to provide enriched, functional human T cell populations. We have found that this method is more rapid, reliable and efficient than other available methods of T cell isolation and/or macrophage depletion. Although the T Lympho-kwik-treated peripheral blood mononuclear cells (PBMC) are enriched for T cells, devoid of macrophages, and are not activated by the T Lympho-kwik reagent, they can be induced to proliferate, secrete lymphokines and/or develop alloreactive cytolytic T cell activity under appropriate experimental conditions. Hence, T Lympho-kwik treatment of PBMC is a highly effective technique for providing enriched T cells that are free of accessory cells and that can be used for further functional studies.
D000938:Antigen-Presenting Cells / Q000166:cytology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008264:Macrophages / Q000166:cytology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology
D000945:Antigens, Differentiation, T-Lymphocyte