In vitro production of granulocyte-macrophage colony-stimulating activity by murine bone marrow and spleen following vinblastine administration in vivo.
The production of granulocyte-macrophage colony-stimulating activity (CSA) by isolated murine femur shafts, non-dispersed bone marrow and spleens was assessed following administration of Vinblastine (VLB). These organs were removed from 2 h to 10 days post-VLB and allowed to condition endotoxin-free medium for 48 h. CSA activity was assessed using a soft agar cloning system. The data demonstrate that CSA elaboration was maximal 24 h post-VLB, corresponding to the nadir of bone marrow GM-CFC, and 6 h after splenic reached a minimum. No relationship between peripheral blood granulocyte count or serum endotoxin levels were observed.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D007377:Interleukin-3 / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology*
D007377:Interleukin-3; D014747:Vinblastine