[Heterogeneity of strains of Haemophilus influenzae of genital and neonatal origin: analysis of capsular serotypes, biotypes and electrophoretic profiles of external membrane proteins and LPS].
The techniques of capsular serotype, biotype determination and sodium dodecylsulphate polyacrylamide gel electrophoresis of sarcosinate-insoluble outer membrane protein (OMP) and of proteinase K lipopolysaccharide (LPS) preparations were applied to 41 genital and neonatal Haemophilus influenzae isolates. Twelve percent were capsulated (4b, 1a). Distribution of strains between biotypes was similar to that of isolates of other non-systemic pathogenic origin; only one isolate was biotype IV. The OMP profiles showed great variability with 4 group of proteins: a 16-Kd major peptide which was observed in all strains; 27-30-Kd major OMP including one constant 30-Kd peptide present in all strains except one; 32- to 50-Kd major OMP; and 49- to 54-Kd minor OMP. The rough LPS profiles also revealed heterogeneity. In view of the variability observed among H. influenzae strains, it is not possible to establish a relationship between pathogenicity and a macromolecular marker.
D000031:Abortion, Septic / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005831:Genital Diseases, Female / Q000382:microbiology*; D005832:Genital Diseases, Male / Q000382:microbiology*; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D008070:Lipopolysaccharides / Q000032:analysis; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D011247:Pregnancy; D012703:Serotyping
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins
Hétérogénéité des souches de Haemophilus influenzae d'origine génitale et néonatale: analyse des sérotypes capsulaires, des biotypes, des profils électrophorétiques des protéines de membrane externe et du LPS.