Hypersensitivity reactions in the small intestine. VII. Induction of the intestinal phase of murine graft-versus-host-reaction by Lyt 2- T cells activated by I-A alloantigens.
This study has examined the nature of the T lymphocytes and the alloantigens that induce the intestinal phase of graft-versus-host reaction in unirradiated F1 mice. Parental spleen cells were depleted of T cells subsets by treatment with anti-Lyt monoclonal antibodies and complement, and we show that Lyt 2- cells alone induce the increased lymphocytic infiltration of the epithelium that characterizes the intestinal graft-versus-host reaction. Lyt 2- cells are also required to induce some of the associated crypt hyperplasia, but the full crypt changes require both Lyt 2- and Lyt 2+ T cells. In intra-H-2 recombinant congenic F1 mice with graft-versus-host reaction, a disparity at the I-A locus was alone sufficient and necessary for crypt hyperplasia and increased intraepithelial lymphocyte counts, while an I-J incompatibility led to suppression of both these indices. The results support the hypothesis that the intestinal pathology of acute GVHD is induced by class II MHC-restricted delayed-type hypersensitivity effector T cells.
D000818:Animals; D000950:Antigens, Ly; D002453:Cell Cycle; D004848:Epithelium / Q000473:pathology; D006086:Graft vs Host Disease / Q000209:etiology / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006967:Hypersensitivity / Q000209:etiology / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology*; D007583:Jejunum; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010641:Phenotype; D013163:Splenomegaly / Q000209:etiology / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000950:Antigens, Ly; D000949:Histocompatibility Antigens Class II