Biosynthesis of porphyrins and corrins. 1. 1H and 13C NMR spectra of (hydroxymethyl)bilane and uroporphyrinogens I and III.
High-field NMR spectroscopic methods have been applied to study the reactions catalyzed by porphobilinogen (PBG) deaminase and uroporphyrinogen III (uro'gen III) cosynthase, which are the enzymes responsible for the formation of the porphyrin macrocycle. The action of these enzymes in the conversion of PBG, [2,11-13C]PBG, and [3,5-13C]PBG to uro'gens I and III has been followed by 1H and 13C NMR, and assignments are presented. The principal intermediate that accumulated was the correspondingly labeled (hydroxymethyl)bilane (HMB), the assignments for which are also presented.
D000642:Ammonia-Lyases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D045728:Corrinoids; D006836:Hydro-Lyases / Q000378:metabolism*; D011163:Hydroxymethylbilane Synthase / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011165:Porphyrinogens / Q000302:isolation & purification*; D011166:Porphyrins / Q000096:biosynthesis*; D012242:Rhodobacter sphaeroides / Q000201:enzymology; D014576:Uroporphyrinogen III Synthetase / Q000302:isolation & purification / Q000378:metabolism*; D014577:Uroporphyrinogens / Q000302:isolation & purification*; D014805:Vitamin B 12 / Q000096:biosynthesis*
D045728:Corrinoids; D011165:Porphyrinogens; D011166:Porphyrins; D014577:Uroporphyrinogens; C024393:hydroxymethylbilane; D011163:Hydroxymethylbilane Synthase; D006836:Hydro-Lyases; D014576:Uroporphyrinogen III Synthetase; D000642:Ammonia-Lyases; D014805:Vitamin B 12