Stimulation of the hexose monophosphate shunt in bovine ciliary body under oxidative stress.
The effect of t-butyl hydroperoxide (TBHP) on the oxidation of C1-radiolabeled glucose and C6-radiolabeled glucose by bovine ciliary body was investigated in the presence and absence of the glutathione reductase inhibitors nitrofurantoin (NF) and 1,3-bis(2-chloroethyl)-1-nitrosourea [BCNU]. 14CO2 production from 1-14C-glucose was markedly stimulated by TBHP and inhibited by BCNU. Using tissue extracts, BCNU was shown to inhibit both glutathione reductase and glucose-6-phosphate dehydrogenase. These results support the hypothesis that bovine ciliary body has high NADPH regenerating capacity by the hexose monophosphate shunt.
D000818:Animals; D002330:Carmustine / Q000494:pharmacology; D002417:Cattle; D002924:Ciliary Body / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D009582:Nitrofurantoin / Q000494:pharmacology; D010084:Oxidation-Reduction; D010427:Pentose Phosphate Pathway; D010545:Peroxides / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D010545:Peroxides; D009582:Nitrofurantoin; D020122:tert-Butylhydroperoxide; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D002330:Carmustine