An enzyme-linked immunosorbent assay for the detection and quantitation of anti-Jo-1 antibody in human serum.
We have developed an enzyme-linked immunosorbent assay (ELISA) specific for autoantibodies directed against the autoantigen Jo-1 (histidyl-tRNA synthetase) using antigen prepared biochemically from HeLa cells. No other patient sera, including those containing antibodies directed at threonyl-tRNA synthetase or alanyl-tRNA synthetase, reacted in the assay. Screening of sera from 169 patients with a variety of autoimmune and neuromuscular diseases confirmed that anti-Jo-1 antibodies are confined to a subgroup of patients with pure polymyositis, pure dermatomyositis, or myositis associated with another rheumatic disease.
D000974:Antibodies, Antinuclear / Q000276:immunology*; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology / Q000302:isolation & purification; D003882:Dermatomyositis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells / Q000276:immunology; D006643:Histidine-tRNA Ligase / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D009220:Myositis / Q000276:immunology; D009468:Neuromuscular Diseases / Q000276:immunology
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D001324:Autoantigens; C046750:Jo-1 antibody; D006643:Histidine-tRNA Ligase