Clq enhancement of IgG-dependent eosinophil-mediated killing of schistosomula in vitro.
Antibody-dependent eosinophil-mediated cytotoxicity plays a role in host protection against metazoan parasite invasion. We examined a possible role for Clq in eosinophil-mediated cytotoxicity by using a Schistosoma mansoni schistosomula killing system in vitro. The addition of monomeric purified human Clq enhanced IgG-dependent human eosinophil-mediated killing from 1.4-fold to 2.3-fold (mean percent killing 12% +/- 4 vs 21% +/- 4, p less than 0.005) when the immune IgG concentration was low. In contrast, there was no significant enhancement of neutrophil-mediated killing. When the IgG concentration was increased fourfold Clq did not cause enhancement of eosinophil-mediated killing (35% +/- 9 vs 37% +/- 5). Preincubation of eosinophils with type 1 collagen abrogated Clq enhancement of killing, raising the possibility of a receptor-mediated process, which depends upon cellular binding of Clq via the collagenous portion of the molecule. Eosinophils and neutrophils were examined for the presence of Clq receptors by using 125I labeled Clq. Clq binding to both cell types was saturable, reversible, and specific, indicating that binding is through specific receptors. Type 1 collagen inhibited binding of Clq to cells, suggesting that Clq binding is via the collagenous stalk of Clq. The number of receptors was approximately twice as high for eosinophils as compared with neutrophils (1.9 X 10(7) vs 1.1 X 10(7), p less than 0.025). Affinity constants for the two cell types were similar (1.5 X 10(7) vs 1.3 X 10(7). These findings suggest that Clq and receptors for Clq on eosinophils may be important for eosinophil-mediated schistosomula killing.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D002352:Carrier Proteins; D003094:Collagen / Q000494:pharmacology; D003166:Complement Activating Enzymes / Q000276:immunology / Q000494:pharmacology*; D003172:Complement C1 / Q000276:immunology / Q000494:pharmacology*; D015922:Complement C1q; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D018960:Hyaluronan Receptors; D007074:Immunoglobulin G / Q000276:immunology*; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D009504:Neutrophils / Q000276:immunology; D011951:Receptors, Complement / Q000032:analysis; D012550:Schistosoma mansoni / Q000502:physiology*; D013268:Stimulation, Chemical
C087299:C1QBP protein, human; D002352:Carrier Proteins; D003172:Complement C1; D018960:Hyaluronan Receptors; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; C032402:complement 1q receptor; D015922:Complement C1q; D003094:Collagen; D003166:Complement Activating Enzymes