Insoluble and soluble allergens from wheat grain and wheat dust: detection of IgE binding in inhalant and ingestion allergy.
The need for better in vitro testing for wheat allergy particularly involved correlating clinical evidence of Type I hypersensitivity with laboratory detection of specific IgE antibodies in serum. We report here an improvement in this relationship by the use of a modified method for RAST (Radioallergosorbent test), involving nitrocellulose as the solid phase and alkali (or ethanol) for extraction of allergens and treatment of discs. Serum IgE reactions with the full range of wheat grain and dust proteins were studied using this method and the results were related to wheat allergies due to flour ingestion and the inhalation of flour, pollen or grain dust.
D000328:Adult; D000485:Allergens; D001249:Asthma / Q000175:diagnosis*; D004391:Dust / Q000009:adverse effects; D005512:Food Hypersensitivity / Q000175:diagnosis*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008875:Middle Aged; D011852:Radioallergosorbent Test; D014908:Triticum / Q000009:adverse effects*
D000485:Allergens; D004391:Dust; D007073:Immunoglobulin E