Escape of hybridomas from cellular defense mechanisms: an in vitro study using autologous and allogeneic lymphocytes.
This study revealed that a hybridoma cell line made from an adenocarcinoma of the colon and autologous enucleated peripheral lymphocytes shared antigenicity of the fusion partners. The hybrid cells could be grown in BALB c nu/nu mice, forming a solid tumor. Gating out hybridoma cells with both CEA and T3 antigens by means of cytofluorometer cell sorting and using them as target cells in a cytotoxicity assay against autologous and allogeneic lymphocytes, the susceptibility for cell-mediated lysis within the cultured hybridoma cells was already lost. These findings suggest that transferred membrane component(s) (T3 antigen) from enucleated lymphocytes are candidates for functional regulator(s) in cell-mediated lysis.
D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002459:Cell Fusion; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D014183:Transplantation, Heterologous
D000954:Antigens, Surface; D002272:Carcinoembryonic Antigen