Effective tumor immunotherapy directed against an oncogene-encoded product using a vaccinia virus vector.
We have constructed a vaccinia virus recombinant that expresses the extracellular domain of the rat neu oncogene-encoded protein, a 185-kDa transmembrane glycoprotein termed p185. Strain NFS mice immunized with this recombinant virus developed a strong antibody response against the neu oncogene product and were fully protected against subsequent tumor challenge with neu-transformed NIH 3T3 cells. No tumor immunoprotection was found when recombinant virus-immunized mice were challenged with Ha-ras-transformed NIH 3T3 cells. These data indicate that immunization with a single oncogene-encoded antigen can fully and specifically protect animals against tumor cells bearing this antigen.
D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D006239:Haplotypes; D007167:Immunotherapy; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D009857:Oncogenes; D051381:Rats; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology