title: Strains of Schizosaccharomyces pombe with a disrupted swi1 gene still show some mating-type switching.
abstract: The swi1+ gene is necessary for effective mating-type (MT) switching in Schizosaccharomyces pombe. It was cloned on a 4.2 kb genomic DNA fragment. By site-directed integration into the genome and gene disruption experiments it was proved that the swi1+ gene itself and not a suppressor had been isolated. Disruption of the swi1+ gene causes a phenotype identical to that of the original swi1 mutant, i.e. the strain still shows some MT switching. The swi1 gene is unique in the genome and gives rise to a 3 kb mRNA.
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005809:Genes, Regulator; D005812:Genes, Switch; D000072235:Mating Factor; D009154:Mutation; D010455:Peptides / Q000235:genetics; D010641:Phenotype; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D010455:Peptides; D000072235:Mating Factor