Cytotoxic effector function of B lymphoblasts.
EBV-transformed B lymphoblastoid cell lines and clones were cytotoxic in vitro against Actinomycin D-pretreated WEHI 164 sarcoma cells, a system in which mononuclear phagocytes were previously identified as effectors. Similarly, unlike resting B cells, normal B lymphoblasts stimulated for 72 hr with Staphylococcus aureus Cowan I and purified according to the expression of the B cell surface marker B1, demonstrated appreciable cytolytic activity. B lymphoblastoid cells and derived supernatants were cytotoxic for Actinomycin D-pretreated WEHI 164 cells, and killing was inhibited by an anti-lymphotoxin but not an anti-tumor necrosis factor antiserum. Thus, B lymphoblasts have cytotoxic potential, mediated by lymphotoxin or a lymphotoxin-like soluble product.
D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D003609:Dactinomycin / Q000494:pharmacology; D006023:Glycoproteins / Q000502:physiology; D006801:Humans; D007111:Immunity, Cellular; D008222:Lymphokines / Q000502:physiology; D008233:Lymphotoxin-alpha / Q000502:physiology; D009000:Monocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D008222:Lymphokines; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; D003609:Dactinomycin