Interleukin 2 induces rapid phosphorylation of cellular proteins in murine T-lymphocytes.
When quiescent murine T-lymphocyte cells were stimulated by the addition of interleukin 2 (IL-2), they reinitiated DNA synthesis after a lag period of 5 h. Under these conditions, rapid but transient phosphorylation of two cellular proteins with Mr values of 27 000 and 26 000 was detected; maximal phosphorylation occurred within 10-15 min after the addition of IL-2. The protein of Mr 27 000 contained phosphoserine, while the protein of Mr 26 000 contained phosphothreonine.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D007376:Interleukin-2 / Q000502:physiology; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D010766:Phosphorylation; D011493:Protein Kinase C / Q000032:analysis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D007376:Interleukin-2; D011506:Proteins; D004247:DNA; D011493:Protein Kinase C