Modern phosphotriester synthesis of long oligonucleotides and their use in genetic engineering.
Long oligodeoxyribonucleotides synthesized by the modern phosphotriester method, which is based on the use of O-nucleophilic intramolecular catalysis, were applied for the construction of artificial DNAs and directed mutagenesis of some genes.
D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis; D005818:Genetic Engineering / Q000379:methods; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA