Control of hemopoiesis by a bone marrow stromal cell clone: lipopolysaccharide- and interleukin-1-inducible production of colony-stimulating factors.
A stromal cell line, GY30, was cloned from mouse bone marrow adherent cell layers. In culture, GY30 cells sustain the production of granulocyte-macrophage progenitor cells (GM-CFU) but fail to support the survival of pluripotential stem cells (CFU-S). GY30 cells secrete two growth factor activities distinct from interleukin-3 (IL-3), IL-2, and macrophage colony-stimulating factor (M-CSF) but functionally similar to GM-CSF and G-CSF. The production of both CSFs is increased 70- to 200-fold by treating GY30 cells with lipopolysaccharide or IL-1. RNA blot analysis reveals the presence of GM-CSF and G-CSF transcripts and demonstrates that IL-1 regulates the production of both factors at the mRNA level. Further, these studies show that the GM-CSF secreted by GY30 cells is structurally similar to the GM-CSF produced by activated T cells.
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002460:Cell Line; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D006410:Hematopoiesis; D007375:Interleukin-1 / Q000502:physiology*; D008070:Lipopolysaccharides / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA
D003115:Colony-Stimulating Factors; D007375:Interleukin-1; D008070:Lipopolysaccharides