title: Homing of antigen-specific T cells in the Lewis rat. I. Accumulation of antigen-reactive cells in the perithymic lymph nodes.
abstract: A permanent ovalbumin-specific T cell line of "helper/inducer" cell phenotype (W 3/13+, W 3/25+, OX 8-) was used to study the homing pattern in normal untreated Lewis rats. After i.v. injection, the migration of these cells was followed directly by using 51Cr- or [14C]thymidine-labeled cells. In addition, I tried to retrieve the cells from different lymphatic tissues by antigen restimulation. I found that most of the radiolabeled cells migrate to the lung, liver, kidney, and spleen. Other lymphoid tissues such as the thymus and the cervical and mesenteric lymph nodes were almost devoid of such cells with one exception: the perithymic lymph nodes (pt-LN). Twenty-four hours after the cell transfer, viable antigen-specific cells could be recovered from these organs. Within 9 days the pt-LN enlarged, the percentage of W 3/13+ and W 3/25+ T lymphocytes was enhanced, and both relatively high spontaneous and antigen-driven responses were measurable in cell cultures of these lymph nodes. All the effects were observed if viable but not irradiated antigen-specific T blasts were transferred. Moreover, after active immunization, antigen-reactive cells appeared to accumulate not only in the draining but also in the pt-LN. In both experimental situations, the adoptive transfer and the in vivo activation of antigen-specific lymphocytes, the pt-LN appear to play an important role in the homing of such cells.
mesh_terms: D000818:Animals; D002465:Cell Movement; D007114:Immunization; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000166:cytology*; D008213:Lymphocyte Activation; D008221:Lymphoid Tissue / Q000166:cytology; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew / Q000276:immunology*; D011919:Rats, Inbred Strains / Q000276:immunology*; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors
chemical_list: D010047:Ovalbumin