title: Assessment of complement activation during extracorporeal circulation by measurement of complement split C3d.
abstract: The present report summarizes our previous experience with the effect of various dialyzer membranes on leukocyte count, plasma C3d and C5a/C5ades-Arg, and total hemolytic complement. A relationship between the formation of C5a/C5ades-Arg within the dialyzer and hemodialysis leukopenia was demonstrated by comparing different membranes. Arterial plasma C3d was found to be a useful cumulative marker of complement activation during hemodialysis. C3d was shown to be generated in the artificial kidney with kinetics resembling the formation of C5a/C5ades-Arg. Total hemolytic complement transitorily decreased with all membranes, but did not reflect dialyzer biocompatibility. It is suggested that determination of complement-split C3d outdates previous more elaborate estimates of complement activation during hemodialysis.
mesh_terms: D003167:Complement Activation / Q000187:drug effects; D003176:Complement C3 / Q000032:analysis*; D015933:Complement C3d; D003182:Complement C5 / Q000032:analysis; D015936:Complement C5a; D005112:Extracorporeal Circulation; D006801:Humans; D007970:Leukopenia / Q000209:etiology; D006435:Renal Dialysis / Q000009:adverse effects
chemical_list: D003176:Complement C3; D003182:Complement C5; D015933:Complement C3d; D015936:Complement C5a