[Periodontal diseases in adults. Immunohistochemical study of inflammatory cells and of collagen].
An immunohistochemical study was performed on 9 gingival biopsy specimens from patients suffering of a periodontal disease comparatively to 7 control specimens from patients with inflammatory gingival hyperplasia of various causes and from healthy gums. We used mono or polyclonal antibodies as markers of the different types of inflammatory cells (T-1 lymphocytes and their subsets, B-lymphocytes, macrophages, plasma cells). In patients with periodontal disease, the high percentage of transformed T-lymphocytes with a majority of helpers was obvious. In controls, the inflammatory cells were principally B-lymphocytes, macrophages and plasma cells. The characterization of different types of collagen produced by periodontal fibroblasts was performed by specific antibodies (anti I, III, V collagen sera). The respective ratio and the topographic distribution of those collagen substances were different in patients with periodontal disease and controls. In the first eventuality, one could find quite exclusively type I collagen. In the other group, type I collagen was associated with type III sub epithelial and type IV perivasor ar collagen. By means of these findings, the pathogenesis of the periodontal disease was discussed and its immune mechanism was suggested.
D001706:Biopsy; D003094:Collagen / Q000032:analysis*; D005885:Gingival Hyperplasia / Q000276:immunology; D005891:Gingivitis / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007958:Leukocyte Count; D010510:Periodontal Diseases / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes
D006684:HLA-DR Antigens; D003094:Collagen
La maladie périodontale de l'adulte. Etude immunohistochimique des cellules inflammatoires et du collagène.