Cross reactions between porcine, bovine and ovine interleukin-2 preparations.
Optimum conditions for the production of porcine interleukin-2 were found to include a delay of 24 hours before the addition of mitogen. Porcine and bovine interleukin-2 responded optimally in homologous systems whereas bovine interleukin-2 gave a better response in the ovine system than homologous ovine interleukin-2. Interleukin-2 produced from a continuous gibbon cell line reacted well with porcine, ovine and bovine T cell blasts indicating that it could act as a universal growth factor for T cell clones produced from these species.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002999:Clone Cells; D003208:Concanavalin A; D003429:Cross Reactions; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology*; D007962:Leukocytes / Q000378:metabolism*; D012756:Sheep; D013552:Swine; D013997:Time Factors
D007376:Interleukin-2; D003208:Concanavalin A