Effect of unfractionated heparin and a low molecular weight heparin fragment on the DNA synthesis of human thymocytes.
The effect of unfractionated heparin and a low molecular weight heparin fragment was tested on the DNA synthesis of human thymocytes cultured for 5 and 48 h. At 5 h, in the absence of serum, there was a stimulation with the highest concentration of unfractionated heparin, 11.1 mg/ml. At 48 h, there was an inhibition with the highest concentration of both the unfractionated heparin and the heparin fragment. In the presence of serum unfractionated heparin in the highest concentration gave an inhibitory effect at 48 h. With 0.01-1.0 mg/ml of unfractionated heparin, a stimulating effect was obtained instead.
D002648:Child; D004247:DNA / Q000096:biosynthesis*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D013601:T-Lymphocytes / Q000378:metabolism*
D006493:Heparin; D004247:DNA