Hormonal parameters in follicular fluid and the fertilization rate of in vitro cultured oocytes.
Oocytes and matched samples of follicular fluid were obtained from 52 preovulatory follicles aspirated laparoscopically for in vitro fertilization (IVF). Follicular fluid concentrations of estradiol (E2), progesterone (P), testosterone (T), prolactin (HPRL), prostaglandin F2 alpha (PGF2 alpha), prostaglandin E (PGE), protein content, and collagenolytic activity were measured and related to the fertilization rate of oocytes cultured in vitro. High concentrations of P and low levels of T and HPRL were associated with mature, fertilizable oocytes. Levels of PGF2 alpha, PGE, and follicular fluid protein concentrations were similar in both groups. Mean collagenolytic activity was increased in the fertilized oocytes, although no significant difference could be observed. Our data demonstrate a close association between follicular fluid steroid and HPRL concentrations and successful fertilization of oocytes.
D015237:Dinoprost; D004624:Embryo Transfer; D004958:Estradiol / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro; D006728:Hormones / Q000032:analysis*; D006801:Humans; D009865:Oocytes; D006080:Ovarian Follicle / Q000032:analysis*; D011374:Progesterone / Q000032:analysis; D011388:Prolactin / Q000032:analysis; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D011863:Radioimmunoassay; D013739:Testosterone / Q000032:analysis
D006728:Hormones; D011458:Prostaglandins E; D011460:Prostaglandins F; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D015237:Dinoprost