Suppression of in vivo polyclonal IgE responses by monoclonal antibody to the lymphokine B-cell stimulatory factor 1.
The lymphokine B-cell stimulatory factor 1 (BSF-1) has been shown to greatly enhance the differentiation of lipopolysaccharide-activated B cells into IgG1- and IgE-secreting cells in vitro. To determine whether in vivo IgG1 and IgE antibody responses are BSF-1 dependent, the ability of a monoclonal rat IgG1 anti-BSF-1 antibody, 11B11, to affect polyclonal IgG1 and IgE production in mice infected with the nematode parasite Nippostrongylus brasiliensis or injected with a purified goat antibody to mouse IgD was studied. 11B11-containing ascites fluid or purified 11B11 strongly inhibited IgE production in both systems but did not affect IgG1 production, while control ascites or normal rat IgG1 had no IgE-inhibitory activity. These results indicate an important physiologic role for BSF-1 in the generation of IgE antibody responses and suggest means for limiting the production of antibodies responsible for allergic reactions without inhibiting protective antibody responses.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D006133:Growth Substances / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007073:Immunoglobulin E / Q000096:biosynthesis*; D015847:Interleukin-4; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D009349:Nematode Infections / Q000276:immunology; D009559:Nippostrongylus / Q000276:immunology
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D015847:Interleukin-4; D007073:Immunoglobulin E