Role of energy in cellular responses to heat.
We have examined the effect of heat on energy-generating processes and on parameters of bioenergetic status in animal cells. Heat inactivates several processes involved in uptake and metabolism of nutrients. In particular, insulin-stimulated hexose transport in HA-1 fibroblasts and electron transport in blowfly sarcosomes (Bowler, 1981) exhibit thermal sensitivities that reflect the vulnerability to heat of the whole cell or organism. These heat-induced lesions in energy production are not, however, reflected by parameters of energy status in most cells studied. In HA-1 fibroblasts, for instance, over 99% of cells are killed by 45 degrees C heat before a decrease is observed in any parameter of energy status. A general role for energy in cellular responses to heat thus seems unlikely, although the thermal responses of tissues in vivo may differ from those of cells in vitro.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002470:Cell Survival; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004734:Energy Metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006019:Glycolysis; D006358:Hot Temperature; D010101:Oxygen Consumption; D010766:Phosphorylation
D000255:Adenosine Triphosphate