The cytoplasmic ribosomes of Chlamydomonas reinhardtii: characterization of antibiotic sensitivity and cycloheximide-resistant mutants.
In vitro protein synthesis was used to characterize the antibiotic sensitivity of cytoplasmic ribosomes from wild-type and antibiotic-resistant strains of Chlamydomonas reinhardtii. Cytoplasmic ribosomes from two cycloheximide-resistant mutants, act-1 and act-2, were resistant to the antibiotic in vitro. The alteration effected by the act-1 mutation, which was dominant in diploids, was localized to the large subunit of the cytoplasmic ribosomes, but no ribosomal protein alterations were detected using two-dimensional gel electrophoresis. The act-2 mutation, which was semidominant in diploids, was frequently associated with a charge alteration in the large subunit ribosomal protein (r-protein) cyL38 that segregated independently from the antibiotic-resistant phenotype in crosses.
D002696:Chlamydomonas / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D003513:Cycloheximide / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D014176:Protein Biosynthesis / Q000187:drug effects; D012269:Ribosomal Proteins / Q000235:genetics*; D012270:Ribosomes / Q000187:drug effects*
D012269:Ribosomal Proteins; D003513:Cycloheximide