Phospholipid-metabolism of a stimulated murine T cell clone.
The release of IP3 and the incorporation of arachidonic acid into phospholipids was measured in a stimulated murine alloantigen-specific, non-cytolytic T cell clone. While Concanavalin A provoked a sharp increase in IP3, Interleukin 2 had no effect on the production of IP3. An increased reacylation of phospholipids with arachidonic acid was seen within the first 4 hours after addition of Concanavalin A, while an effect upon Interleukin 2 was only observed after 8 hours of incubation with Interleukin 2. A similar retarded response to Interleukin 2 was observed in proliferation experiments. These retarded cell responses may be due to changed properties of IL 2 receptors induced by IL 2.
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D003208:Concanavalin A / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D007376:Interleukin-2 / Q000494:pharmacology; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010743:Phospholipids / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
D005227:Fatty Acids; D007295:Inositol Phosphates; D007376:Interleukin-2; D010743:Phospholipids; D013403:Sugar Phosphates; D003208:Concanavalin A