Decreased reactivity of allosera against target lymphocytes obtained following thermal injury or long-term cyclosporine treatment.
We speculated that two diverse causes of potent cell-mediated immune suppression, cyclosporine (CsA) and thermal trauma, may demonstrate some similar actions, and thus tested whether either could alter antisera reactivity against allogeneic target lymphocytes. Target splenocytes from 40% body surface area full-thickness burned Brown-Norway (BN) rats demonstrated significant (P = 0.004) decreased reactivity (agglutination) with antisera produced across a full allogeneic barrier (RT1 major histocompatibility complex (MHC) and non-MHC) compared to control splenocytes. Depression of allogeneic splenic target cell reactivity against Lewis (LEW)-anti-BN allosera was similarly observed using lymphocytes from long-term CsA-treated rats (P = 0.004). The decreased reactivity induced by burn trauma was transferable to pooled normal splenocytes or blood lymphocytes by preincubation with burn plasma (P less than 0.001), and was confirmed by a cellular enzyme-linked immunosorbent assay (CELISA) (P = 0.003). In summary, a similarity consisting of decreased antibody reactivity against lymphocytes from either burned or long-term CsA-treated animals was demonstrated. These results suggested that lymphocyte cell surface allogeneic determinants and their expression and/or availability were altered by either regimen.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002056:Burns / Q000097:blood / Q000276:immunology* / Q000473:pathology; D003524:Cyclosporins / Q000494:pharmacology*; D007106:Immune Sera / Q000276:immunology*; D007116:Immunization, Passive; D008214:Lymphocytes / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology / Q000473:pathology; D013997:Time Factors
D000906:Antibodies; D003524:Cyclosporins; D007106:Immune Sera