Prostaglandin endoperoxides 21. Covalent binding of levuglandin E2 with proteins.
Levuglandin E2 (LGE2), a gamma-ketoaldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis, binds covalently with ram seminal vesicle microsomes. Totally synthetic 5,6-ditritio-LGE2 was prepared and used to determine that rapid covalent binding of LGE2 (initially 800 microM) occurs with 6.4 microM bovine serum albumin (greater than 10 equiv within 1 min) which approaches saturation (approximately 16 equiv) after 40 min at 37 degrees C.
D000818:Animals; D015232:Dinoprostone; D006863:Hydrogen-Ion Concentration; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D011458:Prostaglandins E / Q000138:chemical synthesis / Q000378:metabolism*; D011485:Protein Binding; D012669:Seminal Vesicles; D012710:Serum Albumin, Bovine; D012756:Sheep
D011458:Prostaglandins E; D012710:Serum Albumin, Bovine; C047684:levuglandin E2; D015232:Dinoprostone