The synthesis of peptides with potential thymic hormone activity: the synthesis of endo-Arg38a-deacetylthymosin beta 10.
A tritetracontapeptide corresponding to the entire amino acid sequence of endo-Arg38a-deacetylthymosin beta 10 was synthesized by a conventional solution method. Seven peptide fragments were assembled, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole-Me2Se in trifluoroacetic acid. In preliminary experiments the synthetic tritetracontapeptide increased the entire peripheral T-cell population and a helper T-cell subset when incubated in vitro with blood which was obtained from a uremic patient with pneumonia, but a suppressor/cytotoxic T-cell subset was unaffected under these conditions. The synthetic endo-Arg38a-deacetylthymosin beta 10 was as active as synthetic deacetylthymosin beta 10 in this in vitro assay.
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002855:Chromatography, Thin Layer; D006801:Humans; D011014:Pneumonia / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D013947:Thymosin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D014511:Uremia / Q000378:metabolism
D000911:Antibodies, Monoclonal; C051977:deacetylthymosin beta(10); D013947:Thymosin