A sensitive method to quantify the terminal differentiation of cultured epidermal cells.
Terminal differentiation of normal and malignant keratinocytes is routinely determined by the ability of these cells to form cornified envelopes after incubation with a calcium ionophore. We have used the human squamous cell carcinoma, SqCC/Y1, to quantify cellular differentiation by the formation of detergent-insoluble protein. The methodology developed employs the metabolic labeling of detergent-insoluble cellular protein with [35S]methionine in the presence of a calcium ionophore. The ratio of filter-retainable radioactivity to that of total cellular protein was shown to be closely correlated to the results obtained by measuring the number of envelope-competent cells when cells were induced to enter a pathway of terminal differentiation in culture by serum deprivation or by treatment with hydrocortisone, and during the inhibition of maturation by either retinoic acid (RA) or epidermal growth factor (EGF). This way of measuring the degree of terminal differentiation of epidermal cells is a relatively simple one that readily allows the simultaneous measurement of multiple samples.
D001769:Blood; D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000473:pathology*; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004817:Epidermis / Q000032:analysis / Q000473:pathology*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis*; D012878:Skin Neoplasms / Q000032:analysis / Q000473:pathology*; D012995:Solubility; D014212:Tretinoin
D003470:Culture Media; D008565:Membrane Proteins; D014212:Tretinoin; D004815:Epidermal Growth Factor; D006854:Hydrocortisone