title: Plasma membrane proteins from human normal and chronic myeloid leukemic granulocytes: identification and partial characterization of the concanavalin A-binding and detergent resistant proteins.
abstract: In this work granulocytes from normal human donors and patients suffering from chronic myeloid leukemia (CML) were externally labeled with 125Iodine, using the Iodogen method. 125Iodine labeled Concanavalin A binding proteins (CBP) and detergent-resistant proteins (DRP) were isolated from the cell lysates and characterized by one- and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D- and 2D-SDS-PAGE). Autoradiographs of the 2D-gels of DRP show seven proteins with Mr 118,000 (spot 1 a), Mr 112,000 (spot 1b), Mr 78,000-85,000 (spot 2), Mr 85,000 (spot 4), Mr 52,000 (spot 3, 3 a and 3 b). Of this set, spot 1 b, 2 and 4 are also present in the autoradiographs of 2D-gels of CBP and, hence, may be considered to be transmembrane components. Spot 4 is expressed more intensely in the normal granulocytes while spots 3 a and 3 b are mainly expressed on the leukemic granulocytes.
mesh_terms: D001345:Autoradiography; D002458:Cell Fractionation; D003599:Cytoskeleton / Q000502:physiology; D003902:Detergents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006098:Granulocytes / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D007951:Leukemia, Myeloid / Q000097:blood*; D008565:Membrane Proteins / Q000032:analysis / Q000302:isolation & purification; D008970:Molecular Weight; D010449:Peptide Mapping; D011952:Receptors, Concanavalin A / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D003902:Detergents; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D011952:Receptors, Concanavalin A; D013501:Surface-Active Agents; C027774:concanavalin A-binding glycoproteins; D012967:Sodium Dodecyl Sulfate