Mutations affecting expression and maintenance of genes encoding the serotype b capsule of Haemophilus influenzae.
Deletion mutagenesis analysis of a duplicated gene necessary for Haemophilus influenzae serotype b capsule expression showed that only one functional copy of this gene is required for capsule production and for virulence in infant rats. Mutant strains generated in this study differed from each other and from the parental strain in their ability to maintain the large tandem duplication which contains the genes involved in serotype b capsule expression.
D000818:Animals; D002874:Chromosome Mapping; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005838:Genotype; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D011995:Recombination, Genetic; D012703:Serotyping; D014774:Virulence
D011135:Polysaccharides, Bacterial