Measurement of picogram quantities of rat IgE from in vitro culture supernatants of B lymphocytes in experimental helminth infections.
A double-antibody liquid-phase radioimmunoassay was used to measure picogram quantities of rat IgE. The sensitivity of this method (approximately 10 pg/ml) allowed the determination of the production of IgE in in vitro culture supernatants of B lymphocytes of infected animals.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000469:parasitology; D002478:Cells, Cultured; D003429:Cross Reactions; D006373:Helminthiasis / Q000097:blood* / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis*; D008832:Microchemistry; D051381:Rats
D007073:Immunoglobulin E