Use of V beta.8 genes in splenic Lyt-2+ cytotoxic lymphocyte precursors reactive to bm1 or bm14 alloantigen in individual C57BL/6 mice.
The cytotoxic response of cell sorter-purified small Lyt-2+ splenic cytotoxic lymphocyte precursors from 10 individual C57BL/6 mice to mutant class I H-2Kbm1 or H-2Dbm14 allodeterminants was analyzed under limiting dilution conditions. The cytotoxic activity of anti-bm1-specific or anti-bm14-specific cytotoxic T lymphocyte (CTL) populations (selected for a high probability of clonality) was tested against F23 hybridoma cells; F23+ CTL clones lysed F23 hybridoma targets but F23- CTL clones did not. In the C57BL/6 anti-bm1 mixed lymphocyte reaction, 36% (range 29-48%) of the generated CTL clones were F23+; in the B6-anti-bm14 mixed lymphocyte reaction, 45% (range 34-49%) of the generated CTL clones were F23+. Hence, a large fraction of the anti-bm1- or anti-bm14-reactive CTL clones from C57BL/6 mice use V beta.8 genes to construct these allospecific T cell receptor phenotypes, but no extensive variation in the use of V beta.8 genes in the construction of allospecific T cell receptor phenotypes of restricted heterogeneity is found in individual mice of the same strain.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005260:Female; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013154:Spleen / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D007519:Isoantigens; D011948:Receptors, Antigen, T-Cell