Studies on the mechanism of stimulation of T cells by the Mycoplasma arthritidis-derived mitogen. Role of class II IE molecules.
A mitogen derived from the supernatant of broth cultures of Mycoplasma arthritidis (MAS-P) stimulates a proliferative response by normal, unprimed T cells and interleukin 2 production by some, but not all, T cell hybridomas. The response requires an IE-positive accessory cell (AC). The direct participation of IE, and not IA, in this system was confirmed by two sets of experiments. First, L cells transfected with IE, but not IA, provided effective AC function for both normal T cells and the T cell hybridoma DO-11.10. Second, we have taken a more direct approach by showing that purified IE incorporated in liposomes and used to coat glass beads can support the MAS-P response of the DO-11.10 T cell hybridoma in the absence of intact AC or other AC molecules. Although the receptor for IE-MAS-P has not been identified, we have eliminated from consideration two potential T cell recognition structures. Monoclonal antibody to the antigen-major histocompatibility complex specific receptor failed to inhibit the MAS-P response of DO-11.10 or the T cell line LBRM-33. Furthermore, the L3T4 molecule did not appear to be involved since an L3T4-negative variant of DO-11.10 responded well to the mitogen. In addition, we show that both Lyt-2-positive and L3T4-positive T cells respond to this class II-restricted stimulus. Thus, we postulate the existence of a non-T cell receptor, non-L3T4 receptor that recognizes MAS-P in association with a presumed nonpolymorphic region of IE.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens; D000942:Antigens, Bacterial; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006825:Hybridomas / Q000187:drug effects; D007376:Interleukin-2 / Q000096:biosynthesis; D007739:L Cells / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology*; D009174:Mycoplasma / Q000276:immunology*; D011506:Proteins; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D018089:Superantigens; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000941:Antigens; D000942:Antigens, Bacterial; D000945:Antigens, Differentiation, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D007376:Interleukin-2; D008934:Mitogens; C052996:Mycoplasma arthritidis mitogen; D011506:Proteins; D011948:Receptors, Antigen, T-Cell; D018089:Superantigens