Inhibition of an IgE response by secondary B cells of a different isotype.
We found that the synthesis of IgE anti-Ars antibodies is strongly inhibited by the presence of secondary non-IgE-producing cells that are specific for the Ars hapten. Such B cells can be induced by inoculation of a protein-Ars conjugate in CFA. The effect is seen after inoculation of OVA-Ars in CFA followed by KLH-Ars in alum, or, more convincingly, after adoptive transfer of B cells induced by antigen in CFA. Dose-response data indicated that inhibition can be effected by B cells containing noninhibitory numbers of contaminating T cells. Possible synergistic effects of carrier-specific regulatory T cells were ruled out by using a different protein carrier for immunization of donor and recipient mice. The effect was shown to be specific for the hapten used for immunization of donor mice.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D013154:Spleen / Q000166:cytology
D007132:Immunoglobulin Isotypes; D007073:Immunoglobulin E