Discordant induction of tumor-associated antigen, TA-4 and chorionic gonadotropin beta in cultured cervical carcinoma cells.
The CaSki cell line derived from an epidermoid carcinoma of the uterine cervix produces and releases two types of tumor-associated antigen. One is a eutopic antigen, TA-4 and the other is an ectopic antigen, hCG beta-like material. The aim of the present investigation was to elucidate a possible difference in the induction mechanism of production of TA-4 and hCG beta-like material in the CaSki cells in relation to cellular differentiation and gene modulation. The exposure to epidermal growth factor (EGF, 100ng/ml) for two days greatly increased TA-4 production by the cultured CaSki cells without affecting cell growth and immunoreactive hCG beta production. The EGF-stimulated increase in TA-4 production required a lag period of approximately 24 hours. The exposure to sodium butyrate (2.5mM) stimulated immunoreactive hCG beta production by the cells, but decreased TA-4 production. The stimulatory effect of sodium butyrate on immunoreactive hCG beta production occurred only during the exposure to the agent. These discrepant effects of EGF and sodium butyrate on the production of TA-4 and hCG beta-like material by the CaSki cells suggest that a fundamental difference exists in the induction mechanism for the expression of these two types of tumor-associated antigen in cervical squamous carcinoma cells.
D000951:Antigens, Neoplasm / Q000032:analysis*; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000276:immunology*; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000032:analysis*; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006801:Humans; D002583:Uterine Cervical Neoplasms / Q000032:analysis / Q000276:immunology*
D000951:Antigens, Neoplasm; D002087:Butyrates; D006063:Chorionic Gonadotropin; D003470:Culture Media; C036649:tumor antigen TA-4, human; D020148:Butyric Acid; D004815:Epidermal Growth Factor