[Detection of beta-lactamase-producing bacteria from female patients with acute uncomplicated cystitis].
A total of 122 bacterial strains isolated from urine specimens of 113 female patients with acute uncomplicated cystitis were used for the study of beta-lactamase production and their susceptibility to various antimicrobial agents was determined. Beta-Lactamase activity was qualitatively determined by a paper strip acidimetric method with benzylpenicillin as substrate and by chromogenic cephalosporin methods using pyridine-2-azo-p-dimethylaniline cephalosporin or nitrocefin as substrate. Susceptibility to antimicrobial agents, including ampicillin, carbenicillin, cephalexin, cephalothin, gentamicin, minocycline, fosfomycin, pipemidic acid and sulfamethoxazole-trimethoprim was examined by a disc method. The beta-lactamase-producing strains detected by at least one of the three tests were found in 18 of 105 Escherichia coli isolates and in the single strain of Enterobacter cloacae dermidis, 3 Enterococcus faecalis and 1 Staphylococcus aureus produced beta-lactamase. The isolation rate of strains resistant to ampicillin, carbenicillin and cephalothin in the beta-lactamase-producing strains was significantly higher than that in the non-beta-lactamase-producing strains (p less than 0.01). These results suggest that beta-lactamase plays an important role in developing resistance to beta-lactam antibiotics in patients with uncomplicated urinary tract infection as well as complicated infection.
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D002648:Child; D003556:Cystitis / Q000188:drug therapy / Q000382:microbiology* / Q000652:urine; D004352:Drug Resistance, Microbial; D005260:Female; D006801:Humans; D008875:Middle Aged; D001618:beta-Lactamases / Q000032:analysis / Q000096:biosynthesis*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases