Transient gene expression in untransformed lymphocytes.
Conditions for transfection of normal, freshly isolated and mitogen-stimulated spleen cells were established. A sharp optimum of plasmid at 10 micrograms per 1.5 X 10(7) transfected spleen cells per transfection was observed. The Ig promoter and the IgH enhancer activity was found to be restricted to cells of the B lineage also in normal, freshly isolated lymphocytes in vitro.
D000123:Acetyltransferases / Q000096:biosynthesis; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase; D004742:Enhancer Elements, Genetic; D005260:Female; D007136:Immunoglobulins / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism*; D008223:Lymphoma / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D007136:Immunoglobulins; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase