Molecular analysis of X chromosome dosage compensation in Caenorhabditis elegans.
We used a convenient quantitative dot blot assay to measure transcript levels for two X chromosome-linked genes, myo-2 and act-4, in the nematode Caenorhabditis elegans. We show that there is dosage compensation of transcript levels for these two genes between XX hermaphrodites and X0 males and that a mutation in the dpy-21 gene, postulated from genetic analysis to be involved in control of X chromosome expression, can affect these transcript levels in the manner predicted. However, we observe the dpy-21 effects only at some stages of the life cycle and not at others. These results are generally consistent with earlier genetic and molecular evidence.
D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D012734:Disorders of Sex Development; D008040:Genetic Linkage; D008297:Male; D009154:Mutation; D009693:Nucleic Acid Hybridization; D014158:Transcription, Genetic; D014960:X Chromosome
D004247:DNA