title: Role of B cell stimulatory factor 1/interleukin 4 in clonal proliferation of B cells.
abstract: B cell stimulatory factor 1 (BSF-1)/interleukin 4 (IL-4) has striking effects on colony formation in soft agar by small resting B lymphocytes. BSF-1 alone induces colony formation in this cell population, presumably in costimulation with a mitogenic substance present in bacto-agar. In costimulation with anti-IgM antibodies, BSF-1 caused initial proliferation of 8 to 10% of B cells, resulting in a large number of cell clusters (10 to 40 cells/clone) after 3 to 4 days of incubation. However, substantial number of colonies (greater than 40 cells/clone) developed only from these clusters when IL-1 was added to the cultures. Using a modified immunoperoxidase staining technique for the determination of IgM allotype, evidence was obtained that B cell colonies stimulated with BSF-1 are derived from a single progenitor cell. Neutralization of BSF-1 with 11B11 after a culture period of 1 to 4 days inhibits further proliferation of B cell colonies, indicating that the action of BSF-1 extends for several cell generations beyond initial stimulation from the resting state. Furthermore, it is demonstrated that the synergistic action of IL-1 with BSF-1 is confined to the late culture period, indicating a growth-promoting effect by IL-1 for activated B cells.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects; D003114:Colony-Forming Units Assay; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D007075:Immunoglobulin M / Q000276:immunology; D007375:Interleukin-1 / Q000502:physiology; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008805:Mice, Inbred A / Q000276:immunology; D008807:Mice, Inbred BALB C / Q000276:immunology; D008811:Mice, Inbred DBA / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D003470:Culture Media; D007075:Immunoglobulin M; D007375:Interleukin-1; D007378:Interleukins; D008934:Mitogens; C044203:anti-IgM; D015847:Interleukin-4