title: A method to assess the proliferative activity of small numbers of murine peripheral blood mononuclear cells.
abstract: An economical, reproducible method was developed to assess the mitogenic response of small numbers of murine peripheral blood mononuclear cells (PBMC, 10(3)/ml) to phytohemagglutinin (PHA) stimulation in the presence of feeder layer cells (FLC) and interleukin-2 (IL-2). A linear cell dose-mitogenic response relationship was then demonstrated between the activity of PBMC and spleen cells of individual mice (slope = 1.054 +/- 0.037; r = 0.989 +/- 0.003). This would suggest that mice can be used for longitudinal studies because small blood volumes can be obtained frequently over a period of time from individual mice and the PBMC can be assessed for their T cell proliferative activity.
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection; D002455:Cell Division / Q000187:drug effects; D046508:Culture Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D007376:Interleukin-2 / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D012107:Research Design; D013154:Spleen / Q000166:cytology
chemical_list: D007376:Interleukin-2; D010835:Phytohemagglutinins