Cloning and expression of T4 DNA polymerase.
The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.
D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins