Interleukin 1 stimulates platelet activating factor production in cultured human endothelial cells.
Monocyte-derived interleukin 1 (IL 1) induced the platelet activating factor (PAF) production in cultured human endothelial cells (HEC). The product was identified as PAF by the chemical properties, the susceptibility to phospholipase A2 and C and to lipase A1, its behaviour in thin layer chromatography and in high pressure liquid chromatography and the recovery of biological activity tested on rabbit platelets. The action of IL 1 was concentration-dependent and took more than 2 hours to became apparent. Most of the PAF produced was cell-associated and only the 25% of the total was released.
D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000096:biosynthesis* / Q000302:isolation & purification
D007375:Interleukin-1; D008070:Lipopolysaccharides; D010972:Platelet Activating Factor