[Interaction of muscle glycogen phosphorylase B with methotrexate, folic and folinic acids].
The interaction of rabbit skeletal muscle glycogen phosphorylase b with methotrexate, folic and folinic acids has been studied. Microscopic dissociation constant for the glycogen phosphorylase b--methotrexate complex determined by analytical ultracentrifugation is 0.43 mM. A subunit of glycogen phosphorylase b is shown to have two sites for methotrexate binding. AMP and FMN diminish the affinity of glycogen phosphorylase b to methotrexate, whereas glycogen does not influence the methotrexate binding to the enzyme. Methotrexate, folic and folinic acids are found to be inhibitors of the muscle glycogen phosphorylase b. The inhibition is reversible and characterized by positive kinetic cooperativity (the Hill coefficient exceeds one unity). The value of the pterin concentration causing two-fold diminishing of the enzymatic reaction rate increased in the order: folic acid (0.65 mM), methotrexate (1.01 mM), folinic acid (3.7 mM). The antagonism between methotrexate, folic and folinic acids, on the one hand, and AMP and FMN, on the other, is revealed for their combined action.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000494:Allosteric Regulation; D000818:Animals; D002384:Catalysis; D005486:Flavin Mononucleotide / Q000494:pharmacology; D005492:Folic Acid / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D002955:Leucovorin / Q000378:metabolism* / Q000494:pharmacology; D008727:Methotrexate / Q000378:metabolism* / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D006005:Phosphorylases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011817:Rabbits
D000249:Adenosine Monophosphate; D005486:Flavin Mononucleotide; D005492:Folic Acid; D006005:Phosphorylases; D002955:Leucovorin; D008727:Methotrexate
Vzaimodeĭstvie myshechnoĭ glikogenfosforilazy B s metotreksatom, folievoĭ i folinovoĭ kislotami.