Advantages to mutagenesis techniques generating populations containing the complete spectrum of single codon changes.
The limitations of current mutagenesis techniques are analyzed in terms of the number and kinds of codon changes they make and in terms of the population size needed to produce all single or multiple amino acid variants. It is shown how a technique that can alter a single codon of a gene, producing all possible variant codons without affecting the rest of the gene, has certain advantages, if it can be used at each place in the gene in one experiment. Such a technique has advantages when the goals are to understand: (1) how specific structural alterations in a mutant protein cause it to function in a different but specific way, (2) how to predict which amino acids in a protein contact or interact with each other, and (3) why a protein is more or less sensitive to mutational disruption, depending upon the specific mutation. This is because it would generate the maximum number of (1) mutant proteins with different functions, (2) intracistronic suppressor for any starting mutation, and (3) random amino acid substitutions at random places. Furthermore, such a technique could produce useful variants more quickly and on a smaller scale than either evolution or current methods.
D000596:Amino Acids / Q000235:genetics; D003062:Codon / Q000235:genetics*; D004798:Enzymes / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D009154:Mutation; D011336:Probability; D012333:RNA, Messenger / Q000235:genetics*; D013379:Substrate Specificity
D000596:Amino Acids; D003062:Codon; D004798:Enzymes; D012333:RNA, Messenger