T cell suppressor factor from human glioblastoma cells is a 12.5-kd protein closely related to transforming growth factor-beta.
T cell suppressor factor produced by human glioblastoma cells inhibits T cell proliferation in vitro and more specifically interferes with interleukin-2 (IL-2)-dependent T cell growth. Here we report the purification of this factor from conditioned medium of the human glioblastoma cell line 308. Amino-terminal sequence analysis of the 12.5-kd protein demonstrates that eight out of the first 20 amino acids are identical to human transforming growth factor-beta. Purified glioblastoma-derived T cell suppressor factor and transforming growth factor-beta from porcine platelets inhibit both IL-2-induced proliferation of ovalbumin-specific T helper cells and lectin-induced thymocyte proliferation with similar specific activities. If released by glioblastoma cells in vivo, the factor may contribute to impaired immunosurveillance and to the cellular immunodeficiency state detected in the patients.
D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000276:immunology; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D005910:Glioma / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics*; D013491:Suppressor Factors, Immunologic / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D013552:Swine; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D015291:Transforming Growth Factors
D010455:Peptides; D013491:Suppressor Factors, Immunologic; D015291:Transforming Growth Factors