Group C adenovirus DNA sequences in human lymphoid cells.
Human peripheral blood lymphocytes from healthy adults, cord blood lymphocytes, and lymphoblastoid cell lines were screened by hybridization for the presence of group C adenovirus DNA sequences. In 13 of 17 peripheral blood lymphocyte samples from adults, 1 of 10 cord blood samples, and seven of seven lymphoblastoid cell lines tested, results were positive for Group C adenovirus DNA (adenovirus 1 [Ad1], Ad2, Ad5, or Ad6). About 1 to 2% of the lymphocytes carried 50 to 100 viral genome copies per positive cell, as estimated by in situ hybridization. Infectious virus representing all members of group C were recovered, but cultivation in the presence of adenovirus antibody did not cure the cells of free viral genomes. Viral DNA was found in B, T, and N cells but only in 1 of 10 cord blood samples. The results suggest that group C adenovirus infections in childhood result in the persistence of the viral genome in circulating lymphocytes.
D000260:Adenoviruses, Human / Q000235:genetics / Q000254:growth & development*; D000328:Adult; D000367:Age Factors; D001402:B-Lymphocytes / Q000382:microbiology; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D013601:T-Lymphocytes / Q000382:microbiology
D004279:DNA, Viral