Partial primary structures of human and murine macrophage colony stimulating factor (CSF-1).
Approximately 40 amino-terminal residues and 20 internal residues of CSF-1 purified from the media of cultured human pancreatic carcinoma (MIA PaCa) cells and of cultured murine L cells have been identified. Results indicated that the two subunits in each molecule of biologically active CSF-1 are identical in their amino-terminal portions. The twelve amino-terminal residues of MIA PaCa CSF-1 were found to be identical to those of human-urinary CSF-1, suggesting that the polypeptide portions of the two human proteins may be identical. Approximately 75% of the amino acids identified in both MIA PaCa CSF-1 and murine CSF-1 were found to be common to both. No homology to other proteins was observed. This study suggests a subunit polypeptide Mr nearer to 17K than to 26K predicted from cDNA.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000235:genetics / Q000302:isolation & purification; D004247:DNA / Q000235:genetics; D006801:Humans; D007739:L Cells / Q000032:analysis; D051379:Mice; D010190:Pancreatic Neoplasms / Q000032:analysis / Q000235:genetics
D000596:Amino Acids; D003115:Colony-Stimulating Factors; D004247:DNA