Interleukin 1 stimulation of synovial cell plasminogen activator production.
Addition of human monocyte interleukin 1 (IL-1) to cultured human synovial cells can cause an increase in both cell associated (30-fold) and extracellular (40-fold) plasminogen activator (PA) activity. This increase was inhibited by antibody directed against IL-1 and phenylglyoxal. PA activity could be detected 3 h after the addition of IL-1, continued to increase for 24 h and was dependent on RNA and protein synthesis. The molecular weight of the PA produced from the IL-1 stimulated synovial cells was 55,000 +/- 1,000. Mononuclear cell conditioned media (MCCM) also stimulated synovial cells to produce PA. This stimulation was partly inhibited by anti-IL-1 thus suggesting the presence of appreciable IL-1 activity in MCCM. These results could provide clues as to how immune events are linked to cartilage destruction associated with rheumatoid arthritis.
D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology; D010960:Plasminogen Activators / Q000096:biosynthesis*; D013268:Stimulation, Chemical; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D007375:Interleukin-1; D010960:Plasminogen Activators