Kininogens: inhibitors of cysteine proteinases in plasma and a similar inhibitor in chicken egg white.
Isolation, based on their inhibitory properties, is a fast and simple alternative method for the preparation of kininogens from human plasma. Pure native low Mr kininogen can be obtained in a much higher yield than with classical methods, provided that a similarly efficient inhibition of proteolysis is included. Chicken egg white also contains a high Mr inhibitor of cysteine proteinases. It closely resembles low Mr kininogen in its molecular weight, acidic isoelectric point and 1:2 inhibitor to enzyme molar binding ratio. Its relation to chicken serum kininogen is not known.
D000818:Animals; D002645:Chickens; D015853:Cysteine Proteinase Inhibitors; D004529:Egg White / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007704:Kininogens / Q000097:blood* / Q000302:isolation & purification; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000032:analysis* / Q000302:isolation & purification
D015853:Cysteine Proteinase Inhibitors; D007704:Kininogens; D011480:Protease Inhibitors