title: Catecholamine secretion and adenylate cyclase activation in sea urchin eggs.
abstract: The role of neurotransmitters in sea-urchin eggs was investigated by studying their effect on adenylate cyclase of the egg membrane. Maximal stimulation of enzyme activity occurs in the presence of dopamine and GTP. 5-hydroxytriptamine, 5-methoxytriptamine and acetylcholine have no effect on activity, despite a decrease in intracellular cAMP level in eggs treated with 5-hydroxytriptamine antagonists as previously reported (Renaud et al., 1983). High-performance liquid chromatography (HPLC) revealed that dopamine is released from the sea-urchin egg into the external medium following fertilization.
mesh_terms: D008735:5-Methoxytryptamine / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism* / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation; D006160:Guanosine Triphosphate / Q000494:pharmacology; D010063:Ovum / Q000201:enzymology* / Q000378:metabolism; D012617:Sea Urchins; D012701:Serotonin / Q000494:pharmacology; D015053:Zygote / Q000201:enzymology / Q000378:metabolism
chemical_list: D002395:Catecholamines; D012701:Serotonin; D008735:5-Methoxytryptamine; D006160:Guanosine Triphosphate; D000262:Adenylyl Cyclases; D000109:Acetylcholine; D004298:Dopamine