Localization of human mononuclear cell interleukin 1.
The detection and localization of interleukin (IL) 1 in human monocytes was carried out by flow cytometry using monoclonal antibodies to IL-1 alpha and IL-1 beta proteins. IL-1 alpha was detected on the surface of monocytes and the surface expression increased following lipopolysaccharide activation. No demonstrable IL-1 beta protein could be observed on the cell surface by antibody staining, while both IL-1 alpha and IL-1 beta could be visualized intracellularly by the appropriate monoclonal antibodies following acetone permeabilization of the monocytes. Further experiments with cell associated IL-1 revealed that most of the biological activity of human monocytes could be inhibited by affinity purified polyclonal antibodies to IL-1 alpha protein, whereas no inhibitory activity was observed with IL-1 beta specific antibodies. These data support the hypothesis that a differential localization of IL-1 alpha and IL-1 beta exists within human blood-derived monocytes.
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001681:Biological Assay; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D005434:Flow Cytometry; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D007376:Interleukin-2 / Q000096:biosynthesis; D009000:Monocytes / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D007376:Interleukin-2