Production of chemokinetic inhibitory factor (CIF) by normal blood and spleen B lymphocytes.
We have recently reported the partial purification and characterization of of a new lymphokine, the heat-labile chemokinetic inhibitory factor (CIF) which inhibits neutrophil movement. We have also shown that this lymphokine is produced and secreted by cultured B-chronic lymphocytic leukaemia (CLL) cells in vitro. The present study shows that highly purified resting normal B lymphocytes from blood and spleen have the capacity to produce CIF spontaneously. After activation with anti-IgM or EBV-infection the lymphocytes produced a number of other factors, heat-stable chemokinetic inhibitory factors and heat-labile chemokinetic enhancing factors. Supernatants from a collection of human B-cell lines representing different stages of B-cell differentiation were also investigated. None of these cell lines produced CIF. The present results show that the production of CIF is not restricted to the malignant B-CLL cell but is also produced by a subset of normal blood and spleen B cells.
D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors; D006801:Humans; D007945:Leukemia, Lymphoid; D008222:Lymphokines / Q000096:biosynthesis* / Q000097:blood; D008223:Lymphoma; D009101:Multiple Myeloma; D009504:Neutrophils / Q000166:cytology; D013154:Spleen / Q000276:immunology*
D002630:Chemotactic Factors; D008222:Lymphokines