Induction of nonspecific cell-mediated cytotoxic reactivity from non-immune spleen cells treated with aloctin A.
Culturing of mouse spleen cells with aloctin A (Alo A), a lectin having anti-tumor activity, resulted in the induction of cells cytotoxic to syngeneic and allogenic tumor cells in vitro. Alo A-induced killer cells could be generated from spleen cells of natural killer cell-deficient beige mice but not from those of T cell-deficient nude mice. The results of cytotoxicity assay after treatment with antisera plus complement indicated that the killer cells mainly consisted of Thy 1+, Lyt 1-2+, asialo GM1(1)-T cells. Their cytotoxic activity was markedly augmented by the presence of Alo A during the assay for cytotoxicity. The assay of IL2 in the culture fluid from Alo A-treated spleen cells and the effect of dexamethasone (DEX) on IL2 production from Alo A-stimulated spleen cells and on generation of killer cells suggested that generation of Alo A-induced killer cells was closely associated with the amount of IL2 released from the spleen cells in culture.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D007111:Immunity, Cellular / Q000187:drug effects; D007375:Interleukin-1 / Q000096:biosynthesis; D037102:Lectins / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens; D009374:Neoplasms, Experimental / Q000276:immunology; D037121:Plant Lectins; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D037102:Lectins; D008934:Mitogens; D037121:Plant Lectins; C033193:aloctin A; D003165:Complement System Proteins