Purified membrane and soluble folate binding proteins from cultured KB cells have similar amino acid compositions and molecular weights but differ in fatty acid acylation.
A membrane-associated folate binding protein (FBP) and a soluble FBP, which is released into the culture medium, have been purified from human KB cells using affinity chromatography. By NaDodSO4/PAGE, both proteins have an apparent Mr of approximately 42,000. However, in the presence of Triton X-100, the soluble FBP eluted from a Sephadex G-150 column with an apparent Mr of approximately 40,000 (similar to NaDodSO4/PAGE) but the membrane-associated FBP eluted with an apparent Mr of approximately 160,000, indicating that this species contains a hydrophobic domain that interacts with the detergent micelles. The amino acid compositions of both forms of FBP were similar, especially with respect to the apolar amino acids. In addition, the 18 amino acids at the amino termini of both proteins were identical. The membrane FBP, following delipidation with chloroform/methanol, contained 7.1 mol of fatty acid per mol of protein, of which 4.7 mol was amide-linked and 2.4 mol was ester-linked. The soluble FBP contained only 0.05 mol of fatty acid per mol of protein. These studies indicate that the membrane FBP of KB cells contains covalently bound fatty acids that may serve to anchor the protein in the cell membrane.
D000215:Acylation; D000596:Amino Acids / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis*; D002460:Cell Line; D005227:Fatty Acids / Q000378:metabolism; D058973:Folate Receptors, GPI-Anchored; D006801:Humans; D007624:KB Cells; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D011956:Receptors, Cell Surface; D012995:Solubility
D000596:Amino Acids; D002352:Carrier Proteins; D005227:Fatty Acids; D058973:Folate Receptors, GPI-Anchored; D008565:Membrane Proteins; D011956:Receptors, Cell Surface