title: Diverse morphological responses of normal human synovial fibroblasts to mononuclear leukocyte products: relationship to prostaglandin production and plasminogen activator activities, and comparison with the effects of purified interleukin 1.
abstract: Supernatant media from cultured human mononuclear blood leukocytes (MCCM) induced morphological changes in normal human synovial fibroblasts in culture, including the formation of cells with a dendritic or stellate morphology and, less frequently, cells with a striking fenestrated appearance. These changes were fully reversed within 1 h of removing the MCCM. They were inhibited by indomethacin, the glucocorticoids hydrocortisone, prednisolone and dexamethasone, and by colcemid, but not by actinomycin D and only weakly by cycloheximide. The morphological responses to MCCM could be reproduced by MCCM fractions containing interleukin 1-like activity and by purified forms of human interleukin 1 (IL-1), including monocyte-derived IL-1 beta and recombinant IL-1 alpha. These responses were also inhibited by indomethacin, indicating a link with prostanoid production. However, the morphological responses were not related to the stimulation of plasminogen activator activity due to MCCM, MCCM fractions, or IL-1.
mesh_terms: D002478:Cells, Cultured; D003703:Demecolcine / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology*; D009000:Monocytes / Q000502:physiology*; D010960:Plasminogen Activators / Q000502:physiology*; D011453:Prostaglandins / Q000096:biosynthesis; D013583:Synovial Membrane / Q000166:cytology*; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology
chemical_list: D005938:Glucocorticoids; D007375:Interleukin-1; D011453:Prostaglandins; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator; D007213:Indomethacin; D003703:Demecolcine