Determination of 9 alpha, 11 beta-prostaglandin F2 by stereospecific antibody in various rat tissues.
In view of the recent finding that prostaglandin D2 is stereospecifically converted to 9 alpha, 11 beta-prostaglandin F2, an isomer of prostaglandin F2 alpha, a highly specific and sensitive radioimmunoassay for 9 alpha, 11 beta-prostaglandin F2 was developed and applied to determine the content of this prostaglandin in various rat tissues. Antisera against 9 alpha, 11 beta-prostaglandin F2 were raised in rabbits immunized with the bovine serum albumin conjugate, and [3H]9 alpha, 11 beta-prostaglandin F2 was enzymatically prepared from [3H]prostaglandin D2. The assay detected 9 alpha, 11 beta-prostaglandin F2 over the range of 20 pg to 1 ng, and the antiserum showed less than 0.04% cross-reaction with prostaglandin F2 alpha, prostaglandin F2 beta and 9 beta, 11 beta-prostaglandin F2. To avoid postmortem changes, tissues were frozen in liquid nitrogen immediately after removal. The basal level of 9 alpha, 11 beta-prostaglandin F2 was hardly detectable in various tissues of the rat examined, including spleen, lung, liver and brain; although it was found to be 0.31 +/- 0.06 ng/g wet weight in the small intestine. During convulsion induced by pentylenetetrazole, enormous amounts of prostaglandin D2 (ca. 180 ng/g wet weight) and prostaglandin F2 alpha (ca. 70 ng/g) were produced in the brain; however, 9 alpha, 11 beta-prostaglandin F2 was detected neither there nor in the blood. This result demonstrates that the conversion to 9 alpha, 11 beta-prostaglandin F2 is a minor pathway, if one at all, of prostaglandin D2 metabolism in the rat brain.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost; D007106:Immune Sera; D008297:Male; D010433:Pentylenetetrazole / Q000494:pharmacology; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis* / Q000138:chemical synthesis / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000378:metabolism; D014018:Tissue Distribution
D007106:Immune Sera; D011457:Prostaglandins D; D011460:Prostaglandins F; D015237:Dinoprost; D015230:Prostaglandin D2; D010433:Pentylenetetrazole