Colony-stimulating factor produced by murine adrenocortical tumor cells.
Conditioned medium obtained from the adrenocortical LAF1 mouse tumor (Y-1) cell cultures was able to stimulate the proliferation and the differentiation of granulocyte-macrophage precursors in the normal murine bone marrow. Colony-stimulating factor (CSF) was spontaneously produced by Y-1 cells also in serum-free cultures. By two cycles of gel chromatography on Sephadex G-150 of concentrated conditioned medium two peaks of colony-stimulating activity were isolated that corresponded to apparent molecular radii of 100,000 and 29,000, respectively. At this step of purification, the two factors gave a similar dose-response curve, showed a remarkable resistance to the heat treatment and pH changes, and were not extracted by ether. Because Y-1 cells resulted in infection by retrovirus, they provide a useful model to investigate the relations between viral coded information and CSF production.
D000306:Adrenal Cortex Neoplasms / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008970:Molecular Weight
D003115:Colony-Stimulating Factors; D003470:Culture Media