[Primary structure of the 5S rRNA gene and its flanking sequences in diploid Aegilops tauschii Coss].
In genome of diploid aegilops A. tauschii (k773) two types of sequences 420 and 510 b. p. long which hybridize with 5S[32P]rRNA were discovered. Using the plasmid pBR327 the recombinant plasmids pAt5S79 and pAt5S91 containing 5S DNA repeats of Ae. tauschii were constructed. The primary structure of 5S rRNA gene and that of intergene spacer cloned in pAt5S79 was determined. In gene region of Ae. tauschii coding for 5S rRNA, as compared with T. aestivum, there were found 3 base substitutions in positions 24, 36 and 37. The homology of spacer sequences is 79.1%. In 5S rRNA gene of Ae. tauschii GC-content is 53.3%, but in a spacer--48.1%. The terminator of transcription in Ae. tauschii includes 15 AT-base pairs with predomination of T-bases in uncoding chain.
D004247:DNA / Q000235:genetics; D004171:Diploidy; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D006109:Poaceae / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D004247:DNA
Pervichnaia struktura gena 5S rRNK i flankiruiushchikh ego posledovatel'nosteĭ u diploidnogo egilopsa Aegilops tauschii Coss.