Cytoprotection by sucralfate: role of sulfate ions.
Sucralfate and its component, sucrose octasulfate, are both SO4(2-)-containing compounds shown to protect against acid-peptic injury in rabbit and/or cat esophagi. To determine if sulfate ions (SO4(2-) contributed to this protection, a series of in vitro and in vivo experiments were performed in acid-exposed rabbit esophagi. In the Ussing chamber SO4(2-)-containing solutions significantly reduced the acid-induced decline in electrical resistance (R) observed in controls. This effect was unrelated to buffering of H+, accompanying cation or changes in luminal solution osmolality. Protection by SO4(2-) was specific since other divalent (HPO4(2-] or impermeant anions (gluconate-) failed to reduce the acid-induced decline in R. Protection was confirmed in vivo by showing that acid-perfused esophagi exposed to SO4(2-) had less morphologic damage, higher R and lower permeability to 14C-mannitol and H+ than controls. These results indicate that SO4(2-) have a unique protective action against acid injury to esophageal epithelia, and this action appears to explain the cytoprotective properties of sucralfate.
D000818:Animals; D004942:Esophagitis, Peptic / Q000517:prevention & control*; D004947:Esophagus / Q000187:drug effects*; D011817:Rabbits; D013329:Structure-Activity Relationship; D013392:Sucralfate / Q000494:pharmacology*; D013395:Sucrose / Q000031:analogs & derivatives / Q000494:pharmacology; D013431:Sulfates
D013431:Sulfates; D013392:Sucralfate; D013395:Sucrose; C053081:sucrose octasulfate