Human T-lymphocyte-derived megakaryocyte colony-stimulating activity.
Conditioned medium from a T-lymphoblastic cell line (Mo) contains a number of well-characterized hemopoietins. In this paper we demonstrate that Mo cells also release a factor(s) able to stimulate the growth and the differentiation of megakaryocytic progenitors into large-size pure megakaryocytic colonies in plasma clot cultures. Comparison with other sources of human-active hemopoietins shows that Mo-conditioned medium performs better than others, especially for the megakaryocytic lineage. The factor(s) shows strong similarities with human Meg-CSF obtained from a thrombocytopenic patient's plasma, and is distinguishable from the other hemopoietins present in the medium.
D001854:Bone Marrow Cells; D002460:Cell Line; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D058851:GPI-Linked Proteins; D006801:Humans; D007377:Interleukin-3 / Q000502:physiology; D007943:Leukemia, Hairy Cell / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects*; D008562:Membrane Glycoproteins; D000090204:Mesothelin; D008970:Molecular Weight; D010835:Phytohemagglutinins / Q000494:pharmacology; D014176:Protein Biosynthesis; D011506:Proteins; D011696:Purpura, Thrombocytopenic / Q000473:pathology; D013601:T-Lymphocytes / Q000145:classification / Q000378:metabolism*; D013920:Thrombocythemia, Essential / Q000473:pathology
D058851:GPI-Linked Proteins; D007377:Interleukin-3; D008562:Membrane Glycoproteins; D010835:Phytohemagglutinins; D011506:Proteins; D000090204:Mesothelin