Identification of a long form of cystatin from human saliva by rapid microbore HPLC mapping.
Microbore HPLC methodology permits rapid and sensitive mapping of human saliva proteins. Saliva is sampled and processed in less than one hour, greatly reducing the likelihood of artifactual protein degradation. As little as 50 microliters of saliva yields proteins in sufficient quantities and purity to obtain amino terminal sequences directly. By this route we have discovered a 14 kDa protein extremely homologous to Cystatin S, but amino-terminally extended by eight amino acids.
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015853:Cysteine Proteinase Inhibitors; D006801:Humans; D008970:Molecular Weight; D010449:Peptide Mapping; D011480:Protease Inhibitors / Q000302:isolation & purification*; D012463:Saliva / Q000032:analysis*; D013329:Structure-Activity Relationship
D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors