Immobilized arylsulfotransferase.
Arylsulfotransferase was stabilized for storage more markedly by covalent immobilization onto DEAE-cellulose or DEAE-Sephadex. The optimal pH, Km for sulfate donor and thermostability of covalently immobilized arylsulfotransferase were similar to those of the free enzyme. Tyrosine-containing peptides such as cholecystokinin-8-nonsulfate, tyrosine methylester and (Leu)enkephalin as acceptor substrates were effectively sulfated by the immobilized enzyme.
D015239:Arylsulfotransferase; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D013466:Sulfurtransferases / Q000302:isolation & purification*
D004800:Enzymes, Immobilized; D013466:Sulfurtransferases; D015239:Arylsulfotransferase