[Cryopreservation of parathyroid tissue: evaluation of a simplified method].
A simplified, less expensive cryopreservation technique for parathyroid tissue was examined in animal study and on human tissue in vitro. More than 3/4 of all replantations in the rat were successful after cryopreservation. After replantation of one single gland only 1/3 of all animals attained normal parathyroid function. This fact shows the importance of the quantity of transplanted viable tissue. Viability of human parathyroid tissue after cryopreservation was 80-90%. The PTH-production of human parathyroid tissue in vitro after cryopreservation and the suppressibility in different calcium concentrations were similar to normal fresh tissue. All our results prove a good functioning of this simplified cryopreservation method.
D000818:Animals; D002118:Calcium / Q000097:blood; D005260:Female; D005615:Freezing; D006801:Humans; D006961:Hyperparathyroidism / Q000601:surgery*; D007011:Hypoparathyroidism / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D010280:Parathyroid Glands / Q000637:transplantation*; D010281:Parathyroid Hormone / Q000097:blood; D010710:Phosphates / Q000097:blood; D011183:Postoperative Complications / Q000601:surgery; D051381:Rats; D011917:Rats, Inbred Lew
D010281:Parathyroid Hormone; D010710:Phosphates; D002118:Calcium
Kryopreservation von Nebenschilddrüsengewebe: Prüfung einer vereinfachten Methode.