Human chorion cells respond to growth factors but lose steroidogenic capacity in primary monolayer cell culture.
This study has defined a method for preparation and monolayer culture of cells from chorion laeve. Cell number and cell protein content are stable over 7 d in culture. The cells will divide in response to epidermal growth factor in the presence of a supplemented, enriched medium and a collagen matrix, but they lose steroidogenic activity over time in culture. This culture system can be used as the starting point for the development of a chemically defined hormone-supplemented, serum-free culture system for studies of chorion cell differentiation and fetal membrane cell interactions.
D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D003094:Collagen; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004970:Estrone / Q000096:biosynthesis*; D006801:Humans; D011374:Progesterone / Q000096:biosynthesis*
D003470:Culture Media; D004970:Estrone; D011374:Progesterone; D004815:Epidermal Growth Factor; D003094:Collagen