Inhibition of erythroid differentiation of mouse erythroleukemia cells by a macrophage product(s).
Conditioned media from established murine macrophage cell lines (RAW264.7, P388D1, and WEHI-3) incubated with endotoxin in a serum-free medium contain an erythroid inhibitory activity (EIA) that inhibited dimethylsulfoxide-induced erythroid differentiation of mouse Friend virus-transformed erythroleukemia cells. Endotoxin itself has no EIA activity. Partial purification of EIA demonstrated that it is distinct from other macrophage products such as IL-1, TGF beta, ECGF, FGF, G-CSF, hepatocyte stimulating factor, interferon, PDGF, and cachectin/TNF. These findings indicate that EIA is a macrophage product distinct from other monokines.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000037:antagonists & inhibitors; D004731:Endotoxins / Q000494:pharmacology; D004920:Erythropoiesis / Q000187:drug effects*; D006131:Growth Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D015846:Monokines; D011506:Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D003470:Culture Media; D004731:Endotoxins; D006131:Growth Inhibitors; D015846:Monokines; D011506:Proteins; D004121:Dimethyl Sulfoxide