title: Temperature-mediated processes in teleost immunity: binding and mitogenic properties of concanavalin A with channel catfish lymphocytes.
abstract: Low (non-permissive) temperatures inhibit the proliferation responses of channel catfish T cells to stimulation with Con A. The study reported here was undertaken to ascertain if failure to bind Con A at non-permissive temperatures could explain the observed suppression at such temperatures. The influence of temperature on the binding of fluorescein-labelled succinyl Con A to channel catfish T and B cells was studied by cytofluorography. The results indicated that Con A bound equally well at both mitogenically permissive (22 degrees, 27 degrees and 32 degrees) and non-permissive (12 degrees and 17 degrees) temperatures. Hence, temperature effects on mitogen-binding to cell surface receptors cannot readily explain the observed suppression of channel catfish T-cell proliferative responses at non-permissive temperatures.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism* / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005399:Fishes / Q000276:immunology*; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D013696:Temperature
chemical_list: D003208:Concanavalin A