Inhibition of adenylate cyclase by IL 2 in human T lymphocytes is mediated by protein kinase C.
Interleukin 2 (IL 2) inhibited basal as well as PGE2, isoproterenol and forskolin stimulated cAMP production in human T lymphocytes. Although the stimulation of adenylate cyclase by activators of the enzyme was evident in lymphocyte membrane preparations, the inhibitory effect of IL 2 was observed only if cells were pretreated with IL 2 and the membranes activated with Ca++ and ATP. Additionally, when purified protein kinase C was reconstituted into untreated membranes and activated with Ca++ and ATP, both receptor and non-receptor stimulated adenylate cyclase was inhibited. These results suggest that the inhibition of adenylate cyclase in human T lymphocytes by IL 2 is mediated by protein kinase C.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000097:blood*; D002118:Calcium / Q000494:pharmacology; D015232:Dinoprostone; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D007700:Kinetics; D008213:Lymphocyte Activation; D011458:Prostaglandins E / Q000494:pharmacology; D011493:Protein Kinase C / Q000097:blood*; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007376:Interleukin-2; D011458:Prostaglandins E; D000255:Adenosine Triphosphate; D011493:Protein Kinase C; D000262:Adenylyl Cyclases; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium