Macrophage colony-stimulating activity is produced by three different EBV-transformed lymphoblastoid cell lines.
The lymphoblastoid cell lines BLY 9.84, Gl, and Pl constitutively release a colony-stimulating activity (CSA) which specifically stimulates murine macrophage progenitor cells in vitro. The biochemical characterization of BLY 9.84-derived CSA exhibits a molecular size of apparently 150-200 kDa even under dissociating conditions with 6 M guanidine hydrochloride or inhibition of glycosylation. Production of this CSA is inhibited by cycloheximide and its activity is destroyed by reduction with dithiothreitol. Replating experiments give evidence for a functional similarity with CSF-1 (macrophage colony-stimulating factor).
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002472:Cell Transformation, Viral; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D008264:Macrophages; D051379:Mice
D003115:Colony-Stimulating Factors