The primary in vivo murine cytotoxic T cell response to the flavivirus, West Nile.
A protocol for obtaining cytotoxic T cell responses to the flavivirus West Nile (WNV) has been developed in vivo. CBA/H (H-2k) mice were immunized with 10(6) p.f.u. WNV intravenously and their spleen cells used directly in cytotoxic assays. This method reliably produced WNV-immune Tc cells which showed WNV-specific cytotoxic activity on infected L929 (H-2k) target cells. There was inadequate lysis of infected targets by WNV-immune spleen cells when the m.o.i. was less than 100 p.f.u. WNV, or when tertiary mouse embryo fibroblasts, resident peritoneal macrophages or thioglycollate-induced peritoneal macrophages were used as targets. Only L929 cells infected for 16 h with WNV at a m.o.i. of 100 were suitable targets. Cytotoxic activity against WNV-infected target cells was first detected 4 days after immunization, peaked on day 5 and declined rapidly after day 7. An immunizing dose of 10(3) p.f.u. of WNV was adequate for significant cytotoxicity to be detected; however, the cytotoxic response increased with increasing immunizing doses to plateau levels when 10(6) p.f.u. WNV were used. The cells responsible for lytic activity were H-2-restricted, Thy-1+, Lyt-2+, L3T4- and virus-specific with respect to WNV and influenza virus.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D006183:H-2 Antigens / Q000032:analysis; D007111:Immunity, Cellular; D007114:Immunization; D051379:Mice; D008808:Mice, Inbred CBA / Q000276:immunology; D008815:Mice, Inbred Strains / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*; D014902:West Nile virus / Q000276:immunology*
D006183:H-2 Antigens