Isolation and characterization of a 2.2-kb operon preceding the alpha-amylase gene of Bacillus amyloliquefaciens.
A DNA region of 2.8 X 10(3) base pairs (2.8 kb) upstream of the Bacillus amyloliquefaciens alpha-amylase gene has been isolated. This DNA gave rise to a 2.2-kb transcript. The 3' end of the transcript was mapped with S1 nuclease and shown to terminate 49 base pairs upstream of the -35 region of the alpha-amylase promoter. In B. subtilis minicells this 2.2-kb transcript coded for three different polypeptides, thus indicating a polycistronic operon-type structure. The location and the order of the polypeptides were established using DNA deletions. The joining of the 2.2-kb operon to the downstream alpha-amylase gene in the plasmid pUB110 did not have any significant effect on the level of expression of the alpha-amylase.
D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D009876:Operon; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*
D004269:DNA, Bacterial; D000516:alpha-Amylases