Establishment and characterization of murine macrophage hybrids.
Proteose peptone-induced peritoneal macrophages from CBA/J (H-2k) mice have been fused to a hypoxanthine phosphoribosyltransferase-negative variant of the P388D1 (H-2d) murine macrophage cell line. Six hybrid clones were isolated following HAT selection and further characterized. Five of the six clones express class I antigens of both parental haplotypes by microelisa and by flow cytometric analysis. Class II antigen expression of both haplotypes was apparent following a 72-hr incubation of the hybrids with concanavalin A-stimulated rat spleen cell supernatant. However, I-Ad was expressed in all hybrids to a greater extent than I-Ak. Three clones with the highest level of I-Ak expression, E5, C2, and C4, were capable of antigen presentation to the I-Ak-restricted T-cell line, D10.G4.1. LPS induction of the hybrids resulted in a 2- to 15-fold increase in the amount of IL-1 produced relative to the P388D1 parent. Finally, in distinction to P388D1, all hybrids demonstrated increased Fc-mediated erythrophagocytosis of chromium-labeled antibody-coated erythrocytes. These murine macrophage hybrids appear stable and should serve as useful models in understanding the regulation of macrophage function.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000953:Antigens, Protozoan; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D006183:H-2 Antigens / Q000032:analysis; D006822:Hybrid Cells / Q000166:cytology*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis; D008264:Macrophages / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D010587:Phagocytosis; D015800:Protozoan Proteins
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D006183:H-2 Antigens; D007375:Interleukin-1; D015800:Protozoan Proteins; C043323:immobilization antigens; D007041:Hypoxanthine Phosphoribosyltransferase