Translational fusion with a secretory enzyme as an indicator.
A novel type of translational fusion system has been developed by using a secretory protein, staphylococcal beta-lactamase, as an indicator. The beta-lactamase structural gene was modified to provide N-terminal extensions of 13 and 162 amino acids, and in both cases, the fusion protein was processed and the mature active enzyme was secreted; thus, the expression of a particular upstream gene can be analyzed by monitoring the beta-lactamase activity.
D001665:Binding Sites; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013211:Staphylococcus aureus / Q000235:genetics*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic; D001618:beta-Lactamases / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D001618:beta-Lactamases