Two different cis-active elements transfer the transcriptional effects of both EGF and phorbol esters.
Short cis-active sequences of the rat prolactin or Moloney murine leukemia virus genes transfer transcriptional regulation by both epidermal growth factor and phorbol esters to fusion genes. These sequences act in a position- and orientation-independent manner. Competitive binding analyses with nuclear extracts from stimulated and unstimulated cells suggest that different trans-acting factors associate with the regulatory sequence of each gene. A model is proposed suggesting that both epidermal growth factor and phorbol esters stimulate the transcription of responsive genes via discrete classes of hormone-dependent, enhancer-like elements that bind different trans-acting factors, even in the absence of hormone stimulation.
D000818:Animals; D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005809:Genes, Regulator; D005814:Genes, Viral; D008979:Moloney murine leukemia virus / Q000235:genetics*; D011388:Prolactin / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D004815:Epidermal Growth Factor; D011388:Prolactin; D013755:Tetradecanoylphorbol Acetate