Development of an animal model using a closed system to study the sensitivity of a radiopharmaceutical for the detection of gastrointestinal bleeding.
An experimental animal model using a closed system to study the sensitivity of radiopharmaceuticals for the detection and localization of gastrointestinal (GI) bleeding site in a sheep was developed. This model was validated with 99mTc-DTPA. Radioactivity as low as 85.47 +/- 13.32 kBq in a volume of 2.1 +/- 0.14 ml at a bleeding rate of 0.07 ml/min was detected. Simulated intermittent bleeding experiments indicated that at 1 h after injection of 99mTc-DTPA there was still enough circulating radioactivity to bleed into the gut and that it was possible to perform repeat injection of 99mTc-DTPA as early as 2 h after the first injection.
D000818:Animals; D004195:Disease Models, Animal; D006471:Gastrointestinal Hemorrhage / Q000000981:diagnostic imaging*; D011877:Radionuclide Imaging; D012756:Sheep