Immunochemical detection of cell cycle synchronization in a human erythroleukemia cell line, K562.
Cells of the human erythroleukemic line K562 can be induced by manipulation of culture conditions to arrest within the G1 phase of the cell cycle, and subsequently to enter S phase synchronously after release from G1. Cell cultures subjected to serum deprivation and hydroxyurea (HU) treatment demonstrated less than 5% of the cells to be in S phase. Four hours after release from HU, 63% of the cells were in S phase, as detected by immunofluorescent staining. This protocol offers a method for synchronization of K562 cells at the G1/S border and a technique for detection of S-phase cells without the use of radioisotopes or flow cytometry instrumentation.
D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D004261:DNA Replication; D006651:Histocytochemistry; D006801:Humans; D007399:Interphase; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*