A comparison study between ragweed-specific IgG4 as measured by RAST and FAST.
A solid phase Sepharose-based RIA (IgG4 RAST) was compared to the IgG4 FAST enzyme immunoassay for measurements of Ragweed-specific IgG4. Although FAST reagents were used, a standard serum of known antibody content along with modifications in the FAST assay were necessary to attempt to develop a quantitative assay. Various assay parameters such as precision, reproducibility, sensitivity and parallelism were examined. Specificity of the monoclonal anti-IgG4 used in the FAST was not evaluated. Conditions and method of assay for the two systems differed substantially, particularly in the short incubation times for the IgG4 determinations.
D000485:Allergens / Q000276:immunology*; D003888:Desensitization, Immunologic; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification; D010865:Pilot Projects; D011852:Radioallergosorbent Test / Q000379:methods; D012882:Skin Tests
D000485:Allergens; D007074:Immunoglobulin G; D007073:Immunoglobulin E