Differential expression of the HLA-B7 and the HLA-A2 gene in transfected mouse L(tk-) cells after stimulation by mouse interferon.
Mouse L(tk-) cells were transfected with recombinant genomic clones encoding the human major histocompatibility antigens HLA-A2 or HLA-B7. The exposure of 15 different transfected cell clones to mouse interferon resulted in an up to 2.9-fold enhancement of the HLA-A2 antigen at the cell surface but in an up to 5.5-fold enhancement of the HLA-B7 antigen as shown by quantitative radioimmunoassay with monoclonal antibodies directed against different HLA epitopes. Using the HLA-Bw6 specific monoclonal antibody 2BC4, an even higher increase of the HLA-B7 antigen (up to 12-fold) could be observed. This higher inducibility of an HLA-B versus HLA-A locus gene may reflect distinct regulatory mechanism controlling the expression of HLA class I subregion antigens.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003114:Colony-Forming Units Assay; D005786:Gene Expression Regulation; D006680:HLA Antigens / Q000096:biosynthesis* / Q000235:genetics; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D007370:Interferon Type I; D011994:Recombinant Proteins