Cytotoxic human HLA class II restricted purified protein derivative-reactive T-lymphocyte clones. IV. Analysis of HLA restriction pattern and mycobacterial antigen specificity.
Human T-lymphocyte clones specific for antigenic components of purified protein derivative (PPD) of tuberculin were generated by limiting dilution using in vitro PPD-activated peripheral blood mononuclear cells from a single donor. The HLA restriction specificity of eight clones that were cytotoxic against autologous PPD-pulsed monocyte targets, was examined against a panel of allogeneic PPD pulsed targets. In agreement with our findings with bulk-expanded PPD-reactive cytotoxic T lymphocytes, all clones were restricted by HLA class II antigens: seven by HLA-DR 2 and one by HLA-DRw10--the other HLA-DR antigen of the donor. All clones were CD3+, CD4+, CD8-. One clone exhibited, in addition to HLA-DR2 restriction, unrestricted cytotoxic alloreactivity against HLA-DR1. In monoclonal antibody-blocking experiments the latter clone was the only one that was blocked. Its lytic ability was abolished by two monoclonal antibodies against monomorphic HLA-DR determinants. The antigen specificity of the clones was studied by using autologous monocyte targets pulsed with antigens prepared from a range of different mycobacterial species. All seven HLA-DR2-restricted clones reacted with the majority of antigens tested. In contrast, the HLA-DRw10-restricted clone reacted exclusively with an antigen unique to PPD.
D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000187:drug effects; D000942:Antigens, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D001667:Binding, Competitive; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000276:immunology; D006681:HLA-D Antigens / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D009161:Mycobacterium / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000187:drug effects / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006680:HLA Antigens; D006681:HLA-D Antigens; D014373:Tuberculin