Isolation and characterisation of lactoferrin separated from human whey by adsorption chromatography using Cibacron Blue F3G-A linked affinity adsorbent.
A new one-step procedure for isolating lactoferrin from human whey using Cibacron Blue affinity chromatography is described. Characteristics of the isolated lactoferrin include pI 5.5-6.1 and differential migration on SDS-polyacrylamide gel electrophoresis of apo and iron saturated lactoferrin. The concentration of lactoferrin in breast milk varies with time after parturition but the iron saturation remains relatively constant with time.
D002846:Chromatography, Affinity; D004396:Coloring Agents; D003126:Colostrum / Q000032:analysis*; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000302:isolation & purification*; D007782:Lactoglobulins / Q000302:isolation & purification*; D007785:Lactose / Q000032:analysis*; D008895:Milk, Human / Q000032:analysis*; D011247:Pregnancy; D014227:Triazines
D004396:Coloring Agents; D007782:Lactoglobulins; D014227:Triazines; C012842:Cibacron Blue F 3GA; D007781:Lactoferrin; D007785:Lactose