Augmentation of lymphokine-activated killer cell activity in vitro by aloctin A.
Culturing of mouse spleen cells with IL2-containing MLA144 conditioned medium (MLA144 CM) resulted in the induction of cells cytotoxic to syngeneic and allogeneic tumor cells in vitro. The cytotoxicity was markedly augmented by the presence of aloctin A (Alo A), a lectin having anti-tumor activity, during the assay for the cytotoxicity. The results of cytotoxicity assay after treatment with antisera and complement indicated that the killer cells mainly consisted of Thy 1+, Lyt 1-2-, asialo GM1-T cells.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D037102:Lectins / Q000494:pharmacology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology; D037121:Plant Lectins; D013154:Spleen / Q000276:immunology
D007376:Interleukin-2; D037102:Lectins; D037121:Plant Lectins; C033193:aloctin A