Transcription expressed by isolated cell nuclei in relation to the cell cycle.
In vitro transcription of stimulated T lymphocyte nuclei was studied using a previously described method for evaluating the accumulation of double labelled transcription products. Whereas RNA elongation was inhibited during the G0 and S phases of the cell cycle, the synthesis of short-living products (probably products of abortive initiation) still proceed in vitro. Release of the elongation block with concommitant decrease of short-product synthesis was observed during the G1 phase.
D002453:Cell Cycle; D002467:Cell Nucleus / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000502:physiology; D013698:Templates, Genetic; D013997:Time Factors; D014158:Transcription, Genetic