[Induction of a cytotoxic factor into the peritoneal fluid (PCF) by OK-432. II. Physicochemical and immunological characteristics of PCF].
A cytotoxic factor (PCF = peritoneal cytotoxic factor) was strongly induced by the injection of LPS into the peritoneal fluids of mice which had been previously primed with OK-432. The similarity in biological activity of PCF, TNF and lymphotoxin led us to study the relationships among the three. When incubated with LPS, the macrophages from the mice primed with OK-432 induced PCF, whereas the lymphocytes did not. These results indicate that PCF is different from lymphotoxin. PCF appears to be identical to Tumor Necrosis Factor (TNF) in the serum for the following reasons: The two factors are similar in their modes of cytotoxic action in vitro. Both factors have a tumor-necrotizing effect when injected into tumor-bearing mice. Both are produced from macrophages. They are similar in their physicochemical characteristics. The cytotoxic activity of PCF was totally abolished by anti-TNF serum.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology*; D001688:Biological Products / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008233:Lymphotoxin-alpha / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D010844:Picibanil / Q000494:pharmacology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha
D001688:Biological Products; D006023:Glycoproteins; D008070:Lipopolysaccharides; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; D010844:Picibanil