Influence of various factors on binding of 67Ga to polymorphonuclear leukocytes.
We have studied in vitro, factors that influence the uptake of 67Ga-citrate by human polymorphonuclear leukocytes. Citrate at 20 mM concentration decreased the uptake to 1% of control values. Uptake increased as a function of increased microCi of 67Ga/10(7) cells added and incubation time from 0 to 120 min. Uptake decreased somewhat as the incubation pH was lowered from 7.4 to 6.0. Our results suggest that, in vivo, these cells would accumulate 67Ga as the inflammatory lesion progresses while the acidic milieu would modestly reduce uptake.
D002951:Citrates / Q000494:pharmacology; D005710:Gallium Radioisotopes / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D009504:Neutrophils / Q000378:metabolism*; D009571:Nitrilotriacetic Acid / Q000494:pharmacology; D013696:Temperature
D002951:Citrates; D005710:Gallium Radioisotopes; D009571:Nitrilotriacetic Acid