High-performance liquid-chromatographic assay for prostaglandins with the use of p-(9-anthroyloxy)phenacyl bromide.
Gastric mucosa of rats and swine was incubated in buffer for 1 min to produce prostaglandins (PGs). After extraction and derivatization with p-(9-anthroyloxy)phenacyl bromide, the prostaglandin esters were determined by high-performance liquid chromatography. A 20-microliter sample was injected into a microparticulate silica gel column with a mobile phase of dichloromethane-acetonitrile-methanol (90:9:1). At a flow-rate of 1.5 ml/min the retention times of the prostaglandin esters were 7.14 min (internal standard), 7.90 min (PGF2), 10.05 min (thromboxane2), 12.26 min (6 alpha-keto-PGF1 alpha) and 13.98 min (PGF2 alpha). In spite of high sensitivity (0.1 ng per sample) for PGE2 and PGF2 alpha, only PGE2 synthesis was observed.
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000098:Acetophenones; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost; D015232:Dinoprostone; D005753:Gastric Mucosa / Q000032:analysis; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D011453:Prostaglandins / Q000032:analysis*; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D051381:Rats; D013552:Swine; D013929:Thromboxane B2 / Q000032:analysis
D000098:Acetophenones; D007202:Indicators and Reagents; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C051154:panacyl bromide; D015237:Dinoprost; D015232:Dinoprostone