Differential expression of RT1 class II genes in fibroblast cell lines: recognition by allogeneic and xenogeneic T lymphocytes.
A cosmid (cos a 13.1) containing RT1 class II B alpha and B beta genes was introduced into mouse and rat fibroblast cell lines by transformation. Mouse L cells transformed with cos a 13.1-synthesized cell surface class II molecules that were similar, with respect to apparent molecular weight and binding of xenogeneic- and allogeneic-specific antibodies, to class II molecules on rat B cells. RT1 class II molecules on the surface of mouse L cells were recognized by both allogeneic and xenogeneic T cells. In contrast, rat-2 cells transformed with cos a 13.1 did not synthesize any detectable RT1 class II molecules at the cell surface, and the levels of B alpha and B beta mRNA were generally very low or undetectable. This differential expression of exogenous class II genes was not exclusively due to a trans-acting positive regulatory factor in L cells. Other possible explanations for this difference are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000235:genetics*; D007518:Isoantibodies / Q000276:immunology; D008198:Lymph Nodes; D051379:Mice; D051381:Rats; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014170:Transformation, Genetic
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D007518:Isoantibodies; C038353:histocompatibility antigens RT, rat