Sero-epidemiological survey for alveolar echinococcosis (by Em2-ELISA) of blood donors in an endemic area of Switzerland.
Sera from 17,166 blood donors living in 10 cantons of northern Switzerland in an area endemic for Echinococcus multilocularis were investigated by serological survey for alveolar echinococcosis (AE). A highly species-specific antigen (Em2) and a commonly used E. granulosus hydatid fluid antigen (EgHF) were compared for their suitability in seroepidemiology. EgHF showed a degree of nonspecificity which did not allow direct detection of AE cases. Antibody reaction with Em2 resulted in the detection of 2 asymptomatic clinical cases of AE (seroprevalence 0.01%) within this population of blood donors. A further 4 persons were positive in Em2-ELISA. These 4 persons had negative imaging studies and will be followed serologically and clinically.
D000328:Adult; D000818:Animals; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D001782:Blood Donors; D004444:Echinococcosis, Hepatic / Q000453:epidemiology* / Q000276:immunology; D004446:Echinococcus / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D013557:Switzerland
D000947:Antigens, Helminth; C000594589:Em2 antigen, Echinococcus multilocularis