Contractions of postmortem human saphenous veins perfused with pulsatile flow.
The aim of this study was to evaluate the contractions of postmortem human saphenous veins under pulsatile flow conditions as a simulation of coronary-aortic bypass graft (CABG) surgery. Twenty-five cylindrical specimens of veins, obtained from 20 cadavers several hours after death, were mounted in a pulsatile flow system with a pulse rate of 80/min and a mean flow rate of 7 ml/min at various perfusion pressures. Prostaglandin F2 alpha(PGF2 alpha) was then applied to the outer physiological salt solution at a concentration of 3 X 10(-6) mol. Of the 25 veins, 18 (72%) contracted and seven did not. Contractions were observed at a mean perfusion pressure of less than 60 mmHg but not at higher pressures. Three contraction patterns were observed: One caused pressure gradients between the proximal and distal sites of the vein and showed periodic contractions (P); one showed only tonic contractions (T); the other showed pulse pressure increase without developing the pressure gradients (PP). The incidences of P, T, and PP in the 18 instances of contraction were 50%, 33%, and 17%, respectively. Repeated applications of PGF2 alpha to the same vein with the same and/or increased perfusion pressure caused changes of pattern in the direction of P to T, to PP, and to no response, whereas decreasing perfusion pressure caused the patterns to change in the reverse direction. The pressure gradients that developed in veins showing P and T patterns correlated well with the mean perfusion pressure (r = 0.68, P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
D000368:Aged; D001783:Blood Flow Velocity; D001794:Blood Pressure; D001026:Coronary Artery Bypass; D015237:Dinoprost; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects; D010477:Perfusion; D011180:Postmortem Changes; D011460:Prostaglandins F / Q000494:pharmacology; D011673:Pulsatile Flow; D012212:Rheology; D012501:Saphenous Vein / Q000502:physiology*
D011460:Prostaglandins F; D015237:Dinoprost