The effect of 1,25-dihydroxyvitamin D3 treatment on calcitonin and calcitonin gene-related peptide mRNA levels in cultured human thyroid C-cells.
We utilized the TT cell, a human C-cell line derived from a medullary thyroid carcinoma, to study the effects of 1,25-dihydroxyvitamin D3 on cell growth and expression of the calcitonin gene. The growth rate of cells treated for 8 days with 1,25-dihydroxyvitamin D3 did not differ significantly from control or 24,25-dihydroxyvitamin D3 treated cells. Total RNA was isolated, and calcitonin and calcitonin gene-related peptide (CGRP) levels were measured by hybridization. 1,25 D3 lowered calcitonin and CGRP mRNA levels in a time- and dose-dependent fashion; 24,25 D3 had no effect. Northern blots revealed a decrease in the mature mRNA as well as the common precursor forms, indicating a transcriptional effect of 1,25 D3.
D015650:24,25-Dihydroxyvitamin D 3; D002116:Calcitonin / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide; D002117:Calcitriol / Q000494:pharmacology*; D002277:Carcinoma / Q000378:metabolism*; D004100:Dihydroxycholecalciferols / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009479:Neuropeptides / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D004100:Dihydroxycholecalciferols; D009479:Neuropeptides; D012333:RNA, Messenger; D015650:24,25-Dihydroxyvitamin D 3; D002116:Calcitonin; D002117:Calcitriol; D015740:Calcitonin Gene-Related Peptide