Stimulation of murine B cells by the combination of calcium ionophore plus phorbol ester.
The ability of calcium ionophore and phorbol ester to stimulate entry into S phase was assessed during short-term cultures of murine B cells extensively depleted of T cells. Neither ionomycin alone nor PMA alone stimulated DNA synthesis. However, the combination of ionomycin plus PMA acted in synergy to induce initiation of DNA synthesis in murine B cells. A role for protein kinase C was implied by the inability of 4 alpha-phorbol didecanoate to reproduce the effects of PMA. The combination of ionomycin plus PMA also stimulated DNA synthesis in xid-defective B cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D004357:Drug Synergism; D004789:Enzyme Activation; D004987:Ethers / Q000494:pharmacology; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D015759:Ionomycin; D008213:Lymphocyte Activation / Q000187:drug effects*; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D008819:Mice, Nude / Q000276:immunology; D011493:Protein Kinase C / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004987:Ethers; D008222:Lymphokines; D015759:Ionomycin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate