[Microelectrode study of the cellular reactions of the taste bud in the frog Rana temporaria].
Microelectrophysiological studies reveal two types of cells in the taste bud of frog which differ by the level of their membrane potential. During vertical implantation of microelectrode through the apical part of the taste bud, the potential difference in the upper layer amounts to 15 mV. Further implantation of the electrode results in a stepwise decrease of the potential difference up to 27 mV. Cells of the deeper layer are located 12-24 micron lower from the apical surface. Stimulation of cells by solutions of chemical substances is accompanied by cell depolarization, its amplitude being proportional to stimulus concentration. The steepness of depolarization depends on the modality of the stimulus, being maximum for salts. The data obtained suggest that cells of the second layer, with a higher resting membrane potential level, are taste ones.
D000818:Animals; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D011896:Rana temporaria / Q000502:physiology*; D013268:Stimulation, Chemical; D013650:Taste Buds / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
Mikroélektrodnoe issledovanie reaktsiĭ kletok vkusovoĭ pochki liagushki Rana temporaria.