Induction of concanavalin A dose-dependent T-cell growth factor production by insertion of T-cell membrane components into the AKR thymic lymphoma BW 5147.
Sendai virus vesicles were used as vehicles for the insertion of various cell membranes into different cell lines. The transplantation efficiency was measured by using FITC-labelled concanavalin A (Con A) or monoclonal antibodies against the T-cell marker Lyt 2 and the major histocompatibility complex product H-2Db in a fluorescence-activated cell sorter. Results indicate that it is possible to transplant mitogen responsiveness to certain cell types. Con A stimulation of T-cell membrane transplanted BW 5147 showed that it is possible to induce a mitogen dose-dependent T-cell growth factor production. Consequently this method appears to be an attractive model for further study of the properties of the membrane structures involved in mitogen triggering of cells.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism* / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D007376:Interleukin-2 / Q000096:biosynthesis*; D007939:Leukemia L1210 / Q000276:immunology; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000502:physiology*; D011952:Receptors, Concanavalin A / Q000032:analysis / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism*; D013953:Thymus Neoplasms / Q000378:metabolism*
D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D011952:Receptors, Concanavalin A; D003208:Concanavalin A