A c-ras-Ki oncogene is activated, amplified and overexpressed in a human osteosarcoma cell line.
We present a characterization of an activated oncogene which we found to be present in DNA of the OHA osteosarcoma cell line. We identify this tumor oncogene which transforms Swiss mouse 3T3-cells, with c-ras-Ki 2, one of two known members of the Kirsten ras family of human proto-oncogenes. Its structural outlines are given and we show that: 1) a single point mutation causing a substitution of valine for glycine in codon 12 was found by DNA sequencing; 2) the c-ras-Ki gene is amplified and overexpressed in the original OHA tumor cells and its transformants and 3) the gene product is an abnormal form of the p21 protein.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D009857:Oncogenes; D012516:Osteosarcoma / Q000235:genetics*; D011519:Proto-Oncogenes