Kinetic control of the length of very short homopolymeric additions by terminal deoxynucleotidyltransferase.
This work is an investigation of the practicality of kinetic control of the length of very short deoxynucleotide homopolymeric additions by terminal deoxynucleotidyltransferase. For such very short additions, the possibility that terminal deoxynucleotidyltransferase acts differently with each deoxytriphosphate, or shows interaction effects when presented with multiple deoxytriphosphates, was investigated. Different relative rates of priming and different relative rates of subsequent additions were found for each deoxytriphosphate. Each triphosphate reacted uniquely, and one case of interaction was found, with adenosine interfering with cytidine addition.
D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D003839:Deoxyadenosines / Q000378:metabolism; D003841:Deoxycytidine / Q000378:metabolism; D003849:Deoxyguanosine / Q000378:metabolism; D007700:Kinetics; D013379:Substrate Specificity; D013936:Thymidine / Q000378:metabolism
D003839:Deoxyadenosines; D003841:Deoxycytidine; D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase; D003849:Deoxyguanosine; D013936:Thymidine