Characterization of the mouse placental epidermal growth factor receptor: changes in receptor number with day of gestation.
The epidermal growth factor (EGF) receptor is present in the mouse placenta in both a low- and a high-affinity form having approximate values for Ka of 10(7) M-1 and 10(9) M-1, respectively. No significant difference (P greater than or equal to 0.05) in the number of receptors existed between placental membrane preparations from days 10 and 17, both for the low- and the high-affinity receptor. The number of both high- and low-affinity receptors was significantly lower (P less than 0.05) for day 14 placental membranes than for either day 10 or day 17 placental membranes. Cross-linking of 125I-EGF to placental membranes resulted in the specific labelling of two major receptor forms with approximate molecular weights of 170,000 and 154,000, as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
D000818:Animals; D001667:Binding, Competitive; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000032:analysis*; D005260:Female; D005865:Gestational Age; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D051379:Mice; D008970:Molecular Weight; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy
D007457:Iodine Radioisotopes; D004815:Epidermal Growth Factor; D066246:ErbB Receptors