Production of granulocyte colony-stimulating factor by a human melanoma cell line.
We have isolated a human melanoma line (LD-1) from a patient with melanoma and unexplained leukocytosis. The LD-1 cells produced a colony-stimulating factor (CSF) which stimulated primarily granulocytic colonies in human and murine bone marrow cultures. Erythroid burst and mixed colony-stimulating activity was not detected. A single CSF species with a molecular weight of 21,000 was detected in LD-1-conditioned media by G-200 chromatography. Nude mice transplanted with LD-1 tumors developed granulocytosis and had increased blood CSF levels. Messenger RNA from LD-1 cells directed the synthesis of CSF by Xenopus oocytes. Northern blots of LD-1 RNA hybridized strongly with oligonucleotide probes based on the published sequences for human G-CSF, but not with a probe based on the human GM-CSF sequence. Northern blots hybridized with an oligonucleotide probe based on the CSF-1 sequence showed a high-molecular weight band; however, low-molecular weight CSF-1 mRNAs, which are present in the CSF-1-producing cell line MIA-PaCa-2, were not detected in the LD-1 mRNA. The CSF activity of LD-1 cells is best described as human granulocyte CSF.
D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000302:isolation & purification* / Q000494:pharmacology; D006098:Granulocytes; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007964:Leukocytosis / Q000209:etiology; D008297:Male; D008545:Melanoma / Q000032:analysis* / Q000150:complications / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D009368:Neoplasm Transplantation; D010257:Paraneoplastic Syndromes / Q000209:etiology; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D012878:Skin Neoplasms / Q000032:analysis* / Q000150:complications / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000032:analysis
D003115:Colony-Stimulating Factors; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm