title: A study of bovine T-cell subsets in the blood and mammary gland secretions during the dry period.
abstract: Blood and mammary secretions were obtained from cows throughout the dry period. Quantitative and qualitative assays were performed to determine the cell types and cell distributions at weekly intervals from day of dry off until parturition. The total cell counts in secretions increased during involution and remained at high levels until a few weeks prepartum. The macrophages were the predominant cell type in mammary secretions whereas the numbers of lymphocytes were always less than neutrophils or macrophages. Enriched mononuclear cell populations derived from blood and mammary secretions were also evaluated using "T-cell rosette" assays. Changes observed in the relative distribution of three T-cell subsets in secretions did not reflect the dynamics of the cells in the peripheral blood. T-cell subsets that predominated in mammary secretions were the EN+ EAET+ and EN-EAET+ phenotypes. Distinct patterns of migration or differentiation of T-cell subsets were suggested by the changes of subsets observed in mammary secretions collected throughout the dry period.
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D007958:Leukocyte Count; D008264:Macrophages / Q000166:cytology; D008321:Mammary Glands, Animal / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology; D011247:Pregnancy; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification* / Q000166:cytology