Augmentation of interleukin-2 release by cytochalasins.
Augmentation of mitogen-induced release of the T cell lymphokine interleukin-2 (IL-2) occurred using several cytochalasins in coculture with a T cell lymphoma line (JM) or with purified T cells. When added concurrently with mitogen, cytochalasins had no apparent negative effect either on the ability of mitogen to signal IL-2 production or on the total amount produced. Use of cytochalasins that affect submembranous actin in peripheral lymphocytes established a link between cytoskeletal alterations and lymphokine release, although direct membrane perturbation cannot be excluded. These data indicate that an intracellular pool of IL-2 may accumulate in both T cell lines and in purified peripheral T cells and that maintenance of that pool may be affected by alterations of the cytoskeletal elements. Cytochalasins not only increased IL-2 release, but could substitute for phorbol myristic acetate (PMA) in supporting mitogen-signaled IL-2 production. We interpret these data also to indicate that cytoskeletal attachment to the surface molecules that signal IL-2 production is not needed for the activation.
D000818:Animals; D002460:Cell Line; D003572:Cytochalasins / Q000494:pharmacology*; D006801:Humans; D006922:Hylobates; D007376:Interleukin-2 / Q000378:metabolism*; D008223:Lymphoma; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003572:Cytochalasins; D007376:Interleukin-2; D013755:Tetradecanoylphorbol Acetate