Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens.
The correlation between murine lymphocytes blastoid transformation and alkaline phosphatase (AL-P) activity was examined. When murine spleen cells were stimulated with lipopolysaccharide, a B cell mitogen, for 48 h, the AL-P activity was induced in a dose dependent manner. Similarly, stimulation of the spleen cells with Concanavalin A (Con A), a T cell mitogen, induced the AL-P activity. However, when the thymocytes were treated with Con A, only the [3H]dThd uptake was increased. Other B cell mitogens, such as lipid A and fungal B cell mitogens, and T cell mitogen, Pusum sativum agglutinin (PSA) also induced the AL-P activity of spleen cells. Kinetics of [3H]dThd uptake and induction of AL-P was similar. Correlation coefficients between [3H]dThd uptake and AL-P activity were r = 0.98789, p less than 0.01 for LPS, r = 0.99530, p less than 0.01 for lipid A and r = 0.62595, p less than 0.001 for the fungal B cell mitogens. The AL-P activity of the spleen cell was also induced by treatment with Con A sup, which was a supernatant fluid prepared by spleen cell cultured with Con A for 48 h, containing various lymphokines. These findings suggest that the AL-P activity was induced in the case of B lymphocyte blast transformation when stimulated directly with B cell mitogen or indirectly via lymphokines with T cell mitogen. Measurement of the AL-P activity would be a useful method to assay murine blastoid lymphocytes.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D001487:Basidiomycota / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000201:enzymology*; D013601:T-Lymphocytes / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism
D008934:Mitogens; D003208:Concanavalin A; D000469:Alkaline Phosphatase; D013936:Thymidine