In vitro exposure to electromagnetic fields: changes in tumour cell properties.
Two human colon cancer cell lines, Colo 205 and Colo 320 DM, have been studied for their responses to 60 Hz-generated electromagnetic fields (EMF) using soft agar cloning and monoclonal antibody binding assays to assess exposure-induced changes. Cellular responses have been studied after 24 h continuous exposure of cells concurrently to four experimental conditions; i.e. no EMF (E-M-), magnetic field only (M+, 1.0 G rms), electric field only (E+, 300 mA/m2 rms), and combined electric plus magnetic fields at these intensities (E+M+). Under these conditions, both cell lines demonstrated significantly increased colony formation in soft agar and increased expression of tumor associated antigens after exposure to E+M+ and to M+ as compared to unexposed controls.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003110:Colonic Neoplasms / Q000503:physiopathology*; D004261:DNA Replication; D004558:Electric Stimulation; D055590:Electromagnetic Phenomena; D006801:Humans; D051379:Mice; D013499:Surface Properties; D013997:Time Factors
D000911:Antibodies, Monoclonal