Inositol(3,4)bisphosphate and inositol(1,3)bisphosphate in GH4 cells--evidence for complex breakdown of inositol(1,3,4)trisphosphate.
Analysis of inositol bisphosphates in GH4 cells labelled with [3H]myo-inositol shows that these cells contain three detectable inositol bisphosphates: inositol(1,4)bisphosphate, and two novel inositol bisphosphates. These latter inositol bisphosphates were degraded by periodate oxidation, borohydride reduction and alkaline phosphatase dephosphorylation; each yielded single non-cyclic alditols, ribitol and threitol, indicating that they must be respectively inositol(1,3)bisphosphate and inositol(3,4) bisphosphate. These two inositol bisphosphates are putative breakdown products of inositol(1,3,4)trisphosphate, and their occurrence suggests a complex route of hydrolysis of inositol(1,3,4)trisphosphate in intact cells.
D000818:Animals; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D007202:Indicators and Reagents; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000302:isolation & purification* / Q000378:metabolism*; D013403:Sugar Phosphates / Q000302:isolation & purification* / Q000378:metabolism*
D007202:Indicators and Reagents; D007295:Inositol Phosphates; D013403:Sugar Phosphates; C051853:inositol 1,3-bisphosphate; C051854:inositol 3,4-bisphosphate; D015544:Inositol 1,4,5-Trisphosphate