15N labeling of oligodeoxynucleotides for NMR studies of DNA-ligand interactions.
The amino protons of 15N-labeled DNA were studied as a possible structural probe in NMR investigations of the interaction of DNA with various ligands. Since the imino protons are located in the center of the double helix, and variations of their chemical shift values are difficult to interpret in terms of structural changes, these probes are not very useful. Instead, amino protons are located in the major or minor groove of the DNA and are often directly involved in the binding of a ligand. For a selective probing 4-15NH2-2'-deoxycytidine and 6-15NH2-2'-deoxyadenosine were obtained by chemical synthesis. The labeled nucleosides were introduced in distinct positions of oligodeoxynucleotides by large-scale DNA synthesis. Direct 15N NMR and 1H-15N multiple quantum NMR were applied to detect the corresponding 15N labels or protons attached to the 15N labels. Chemical shift values for the cytidine and the adenosine amino nitrogen and proton resonances of a symmetric 18 base pair lac operator sequence are reported.
D000225:Adenine / Q000138:chemical synthesis; D004247:DNA; D003839:Deoxyadenosines / Q000138:chemical synthesis; D003841:Deoxycytidine / Q000138:chemical synthesis; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides
D003839:Deoxyadenosines; D008024:Ligands; D009587:Nitrogen Isotopes; D009838:Oligodeoxyribonucleotides; D003841:Deoxycytidine; D004247:DNA; D000225:Adenine