1,25(OH)2D3 increases cytosolic Ca++ concentration of osteoblastic cells, clone MC3T3-E1.
Effect of 1,25(OH)2D3 in vitro on cytosolic Ca++ concentration of osteoblastic cells (MC3T3-E1) was studied. Marked but transient increase of cytosolic Ca++ concentration of osteoblastic cells was observed following the addition of 10 pg/ml of 1,25(OH)2D3, but not with 10 pg/ml of 24,25(OH)2D3. The increase of cytosolic Ca++ concentration of osteoblastic cells by 1,25(OH)2D3 was not observed when the cells were incubated in Ca++ free medium. Therefore, it was concluded that 1,25(OH)2D3 increased cytosolic Ca++ concentration of osteoblastic cells through the increase of Ca++ influx into the cells.
D015650:24,25-Dihydroxyvitamin D 3; D000634:Aminoquinolines; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002999:Clone Cells; D003600:Cytosol / Q000378:metabolism; D004100:Dihydroxycholecalciferols / Q000378:metabolism; D004987:Ethers / Q000494:pharmacology; D015759:Ionomycin; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*
D000634:Aminoquinolines; D004100:Dihydroxycholecalciferols; D004987:Ethers; D015650:24,25-Dihydroxyvitamin D 3; D015759:Ionomycin; D002117:Calcitriol; C035739:Quin2; D002118:Calcium