Effects of cholesterol and alfa-2-recombinant interferon on cell growth and cell antigen production in the HT 29 cells, an established cell line of human colon adenocarcinoma.
HT 29 cells, an established cell line of human colon adenocarcinoma, were grown in RPMI 1640 medium without or with cholesterol at 25, 50, 100 micrograms/ml concentrations. In some experiments 100 or 200 U/ml alfa-2-A recombinant Interferon were added to the medium. Only in the case of the highest cholesterol concentration there was a reduced number of cells at confluence. Moreover, only the production of CEA increased in the presence of cholesterol. Interferon did not affect cell growth appreciably but stimulated CEA release into the medium during the first three days of culture. Morphological analysis of cells in the presence of cholesterol seems to indicate an attempt of the cells to differentiate.
D000230:Adenocarcinoma / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane; D002784:Cholesterol / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000473:pathology; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008854:Microscopy, Electron; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology*
D002272:Carcinoembryonic Antigen; D007370:Interferon Type I; D011994:Recombinant Proteins; D002784:Cholesterol