title: A factor with interleukin-1-like activity is produced by peritoneal cells from the frog, Xenopus laevis.
abstract: Thymocytes from juevenile Xenopus laevis did not proliferate in response to commercial preparations of lipopolysaccharide (LPS), responded poorly when cultured with the T-cell mitogen, phytohaemagglutinin-P (PHA), and were not co-stimulated by PHA plus LPS. However, supernatants (SNs) from LPS-treated cultures of adult Xenopus macrophage-enriched resident peritoneal cells (PCs) enhanced the proliferative responses of thymocytes to a submitogenic dose of PHA. These SNs were incapable of supporting long-term growth of thymic lymphoblast cell lines, and thus could be distinguished from T-cell growth factor (TCGF)-rich SNs, which were essential for propagating these cells. The co-stimulatory activity was present in 0-24-hr SNs; after 48 hr, SN activity declined. No functional cross-reactivity of mammalian and Xenopus interleukin-1 (IL-1)-rich SNs was detected. These data are consistent with the proposition that a macrophage-derived factor, functionally homologous with mammalian IL-1, can enhance a T-cell proliferative response in an amphibian.
mesh_terms: D000818:Animals; D002474:Cell-Free System; D002478:Cells, Cultured; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology; D014982:Xenopus laevis / Q000276:immunology*
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins