Analysis of human glutamyl- and lysylplasminogen by high-performance affinity chromatography.
An analytical system for the fibrinolytic system in the blood is described, based on high-performance affinity chromatography and a newly devised specific proenzyme detection procedure. Plasminogen subspecies in human plasma without any pretreatment (less than 100 microliters) were specifically separated on an high-performance affinity column. For detection they were continuously activated by urokinase, and their elution profile was determined by assay of the plasmin activity using a specific fluorogenic substrate. Preliminary data on plasma from a patient are presented.
D002846:Chromatography, Affinity; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood; D010446:Peptide Fragments / Q000032:analysis*; D010958:Plasminogen / Q000032:analysis*
D010446:Peptide Fragments; C018998:lysyl-plasminogen; D010958:Plasminogen