Potentiation of neutrophil function by recombinant DNA-produced interleukin 1a.
The effect of interleukin 1a (IL-1a) produced by E. coli-derived recombinant DNA was evaluated on various parameters of human neutrophil function. IL-1a alone stimulated neutrophil hydrogen peroxide production in a dose-dependent manner, but the rate was much lower than that of opsonized zymosan. IL-1a induced release of specific granule contents, but not azurophilic granule contents. Cytochalasin B did not augment the rate of release. IL-1a was chemotactic for neutrophils at the optimal concentrations of 0.1-10 ng/ml. Pretreatment of the neutrophils with IL-1a augmented neutrophil oxygen radical production induced by opsonized zymosan, and this synergistic effect was evident as early as 10 min after IL-1a was added to the neutrophil culture. Phagocytosis of opsonized particles by neutrophils, and degranulation induced by opsonized zymosan were also enhanced by IL-1a in a dose-dependent manner. The present results suggest that IL-1a is weak as a direct activator of neutrophil function and that IL-1a in vivo may augment the response of neutrophils to other stimulators such as foreign bodies.
D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D004274:DNA, Recombinant; D004357:Drug Synergism; D005089:Exocytosis / Q000187:drug effects; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D004274:DNA, Recombinant; D005609:Free Radicals; D007375:Interleukin-1; D011994:Recombinant Proteins; D003571:Cytochalasin B; D015054:Zymosan; D006861:Hydrogen Peroxide