Accessory cell-independent activation of T cells by oxidized red blood cells plus polyethylene glycol.
We have previously shown that the combination of neuraminidase-galactose oxidase (NAGO)-treated autologous erythrocytes (EOX) and polyethylene glycol (PEG) is highly mitogenic for human peripheral blood lymphocytes (PBL). In this report, we show that EOX plus PEG-induced T lymphocyte proliferation is independent of HLA-DR and Leu M3-positive accessory cells (AC). Purified T (pT) cells and PBL were equally stimulated by EOX + PEG, while pT cells were unresponsive to the mitogens phytohemagglutinin (PHA), NAGO, and the anti-CD3 antibody UCHT1, even in the presence of PEG. These findings indicate that specific signals from AC may be replaced by unspecific stimuli in T cell activation.
D000938:Antigen-Presenting Cells / Q000276:immunology; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D005692:Galactose Oxidase / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D009439:Neuraminidase / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D011092:Polyethylene Glycols; D005692:Galactose Oxidase; D009439:Neuraminidase