Interferon: a mediator of indoleamine 2,3-dioxygenase induction by lipopolysaccharide, poly(I) X poly(C), and pokeweed mitogen in mouse lung.
When C3H/He mice were treated with lipopolysaccharide, poly(I) X poly(C), or pokeweed mitogen, the serum interferon titer increased almost instantaneously (100-2000 units/ml), and then the pulmonary indoleamine 2,3-dioxygenase was induced 50- to 140-fold. The peaks corresponding to interferon induction always preceded (approximately 24 h) those corresponding to dioxygenase induction. In C3H/HeJ (lipopolysaccharide-nonresponder) mice, however, lipopolysaccharide was totally inert in induction of both interferon and dioxygenase, although treatment with poly(I) X poly(C) and pokeweed mitogen led to a remarkable increase in the serum interferon titer and the enzyme activity. When lymphocytes of C3H/HeJ mice were inactivated by X irradiation and then reconstituted by the transfer of spleen cells from C3H/He mice, both enzyme and interferon from C3H/HeJ mice thus treated were induced almost normally after the lipopolysaccharide treatment. In addition, murine interferon alpha/beta, which was injected intravenously in C3H/He or C3H/HeJ mice, almost instantaneously and dose-dependently induced the pulmonary enzyme, and at a dose of 10(5) units per mouse the enzyme activity was enhanced 20- to 26-fold in these two strains of mice. These results suggest that interferon, which is generated by the interaction of lymphocytes with lipopolysaccharide, poly(I) X poly(C), or pokeweed mitogen, is a mediator of indoleamine 2,3-dioxygenase induction in the mouse lung by these agents.
D000818:Animals; D004790:Enzyme Induction / Q000187:drug effects; D007370:Interferon Type I / Q000097:blood / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000201:enzymology* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010105:Oxygenases / Q000096:biosynthesis*; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D011070:Poly I-C / Q000494:pharmacology*; D014366:Tryptophan Oxygenase
D007370:Interferon Type I; D008070:Lipopolysaccharides; D011043:Pokeweed Mitogens; D010105:Oxygenases; D014366:Tryptophan Oxygenase; D011070:Poly I-C