EGF inhibits the differentiation of adipocyte precursors in primary cultures.
Adipocyte precursors, isolated from inguinal fat pads of 2-day old NBR rats, proliferate and differentiate in defined medium. EGF stimulates the proliferation of adipocyte precursors with an ED50 of 2 ng/ml and inhibits their differentiation with an ED50 of 0.45 ng/ml. EGF has no effect on differentiated adipocytes. EGF binding studies indicate that adipocyte precursors have two classes of receptors (Kd 4 X 10(-11) M, 4800 receptors/cell and Kd 1.4 X 10(-9) M, 22,000 receptors/cell). TGF-alpha has the same effect as EGF on adipocyte precursors. In contrast, basic and acidic FGF stimulate adipocyte precursor proliferation without inhibiting their differentiation.
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D010455:Peptides / Q000494:pharmacology; D051381:Rats; D013997:Time Factors; D015291:Transforming Growth Factors
D010455:Peptides; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors