Growth-promoting effect of recombinant interleukin 1 and tumor necrosis factor for a human astrocytoma cell line.
IL 1 can exhibit dichotomous effects in the sense that it is cytotoxic for certain cells, although growth promoting for other cells. Because IL 1 is growth promoting for astrocytes, but cytotoxic for melanoma cells, the current investigation evaluated the effect of IL 1 upon astrocytomas. The human astrocytoma U373 was found to incorporate [3H]thymidine after exposure to recombinant human IL 1 alpha and IL 1 beta and murine IL 1 alpha. Surprisingly, U373 also incorporated [3H]thymidine after exposure to recombinant TNF. The response of the U373 to IL 1 may be used as a simple and sensitive assay for IL 1.
D001254:Astrocytoma / Q000473:pathology*; D002455:Cell Division; D005910:Glioma / Q000473:pathology; D006023:Glycoproteins / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D015846:Monokines; D011506:Proteins / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D006133:Growth Substances; D007375:Interleukin-1; D015846:Monokines; D011506:Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha