Effect of multiporous microspheres derived from chitin and partially deacetylated chitin on the activation of mouse peritoneal macrophages.
Multiporous microspheres were prepared from 80% deacetylated chitin (DAC-80) and chitin, and their effects on the activation of murine peritoneal macrophages in vivo and on the production of monokines such as colony-stimulating factor (CSF) and interleukin 1 (IL-1) were examined. Multiporous DAC-80 microspheres of mean diameter 2.5 microns [MS-DAC-80(2.5)] enhanced the cytolytic activity of peritoneal macrophages and the production of CSF in vitro by macrophages, spleen cells and bone marrow cells, and in vivo. MS-DAC-80(2.5) also stimulated the production of IL-1 by both resident and thioglycolate-induced peritoneal macrophages. Multiporous chitin microspheres [MS-chitin(2.5)] showed no effect on the activation of peritoneal macrophages in vivo and on the production of IL-1 in vitro, but slightly enhanced the production of CSF in serum in vivo.
D000107:Acetylation; D000818:Animals; D002686:Chitin / Q000276:immunology*; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D003602:Cytotoxicity, Immunologic; D007375:Interleukin-1 / Q000096:biosynthesis; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008863:Microspheres; D015846:Monokines; D010529:Peritoneal Cavity / Q000166:cytology; D014176:Protein Biosynthesis; D013329:Structure-Activity Relationship
D003115:Colony-Stimulating Factors; D007375:Interleukin-1; D015846:Monokines; D002686:Chitin