Electrokinetic status of the subpopulations of lymph node cells in contact-sensitized CBA mice.
Electrokinetic behaviour of LNC and their subpopulations from CBA mice contact-sensitized with dinitrofluorobenzene and oxazolone were studied. The sensitized LNC showed a significant reduction of mean EPM on the days of maximum DH response. Histogram analysis revealed that LNC of unsensitized as well as contact-sensitized mice were heterogeneous populations. While the LNC of unsensitized mice resolved into two subpopulations, sensitized LNC resolved into three distinct subpopulations. The emergence of a new populations with mean EPM intermediate between those of low and high mobility lymphocytes was a consequence of contact sensitization. Enriched subpopulations were also obtained by nylon adherence-dependent and surface marker-dependent procedures. histograms of these subpopulations revealed that in the mice sensitized to DNFB and oxazolone both T and B cells were electrokinetically altered and were heterogeneous in the distribution of their EPM. These findings are similar to the earlier observations on EPM of LNC in allograft-sensitized mice.
D000818:Animals; D001402:B-Lymphocytes; D002465:Cell Movement / Q000187:drug effects; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology*; D004139:Dinitrofluorobenzene; D004586:Electrophoresis; D008198:Lymph Nodes / Q000166:cytology*; D008214:Lymphocytes / Q000145:classification* / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA / Q000276:immunology*; D010081:Oxazolone; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes
D010081:Oxazolone; D004139:Dinitrofluorobenzene