Anchorage-independent cell growth-inhibiting factor(s) from normal rat liver and ascites hepatoma.
Acid/ethanol extracts of normal rat liver and rat ascites hepatoma cells (AH 130) were found to inhibit the epidermal growth factor (EGF)-dependent colony formation of Balb/c 3T3 cells. The extracts did not inhibit the growth of the 3T3 cells in monolayer culture. The inhibitory activities were heat- and acid-stable and were not inactivated by the treatment with pronase, RNase and DNase. Upon ultrafiltration, a considerable fraction of the inhibitory activities were found in the fraction corresponding to the molecular size ranging from 3.5 to 10 Kd, in which no appreciable TGF-beta activity was detected.
D000818:Animals; D001201:Ascites; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors; D006131:Growth Inhibitors / Q000302:isolation & purification*; D008099:Liver / Q000166:cytology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D051381:Rats; D015291:Transforming Growth Factors
D006131:Growth Inhibitors; D010455:Peptides; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors