Inducible reactivation of UV-irradiated cholera phage e5 in Vibrio cholerae MAK757.
The survival of UV-irradiated cholera phage e5 was found to increase when the host cells, Vibrio cholerae MAK757, were exposed to a low dose of UV irradiation before phage infection (Weigle reactivation), indicating the existence of a UV-inducible DNA repair pathway (SOS repair) in V. cholerae MAK757. The induction signal generated by UV irradiation was transient in nature and lasted about 20-30 min at 37 degrees C. Maximal Weigle reactivation of the phage was obtained when the host cells were irradiated with a UV dose of 16 J/m2. V. cholerae MAK757 was also found to possess efficient photoreactivation and host cell reactivation of UV-damaged DNA in phage e5.
D001435:Bacteriophages / Q000235:genetics / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D007700:Kinetics; D008027:Light; D014466:Ultraviolet Rays; D014734:Vibrio cholerae / Q000235:genetics / Q000528:radiation effects*