Kinetics of [3H]MPP+ uptake in dopaminergic neurons of mouse: regional effects of MPTP neurotoxicity.
In an effort to determine the specificity of MPTP/MPP+ toxicity with respect to the dopaminergic systems, the effect of prior MPTP treatment on [3H]MPP+ uptake in the striatum and olfactory tubercle of BALB/cBy mice was examined. Kinetic analysis of [3H]MPP+ uptake indicated a reduction of Vmax values in both striatum (49%, P less than 0.05) and olfactory tubercule (26%, P less than 0.05). MPTP treatment did not significantly alter the Km in either region, although MPP+ accumulates in both olfactory tubercle and striatum, these dopaminergic systems show different sensitivity to the neurotoxicity of MPTP/MPP+.
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium; D000818:Animals; D001693:Biological Transport, Active; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009830:Olfactory Bulb / Q000187:drug effects / Q000378:metabolism; D011725:Pyridines / Q000633:toxicity*; D011726:Pyridinium Compounds / Q000378:metabolism* / Q000633:toxicity; D013574:Synaptosomes / Q000378:metabolism
D011725:Pyridines; D011726:Pyridinium Compounds; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine