Suppression of cytolytic T-lymphocyte responses through inactivation of non-T accessory cells.
The suppressive properties of nonspecific cytotoxic T lymphocyte (CTL) populations, derived from murine fetal calf serum (FCS)-precultured cells expanded in interleukin 2 (operationally defined as FCS-CM-expanded cells), were investigated on CTL responses generated by syngeneic alloreactive lymphoid cells. Our results suggest that the addition of FCS-CM-expanded cell populations can inhibit the CTL response by elimination of the bone marrow-derived macrophage (BM M phi) population used as non-T accessory cells. Indeed, in the culture conditions used, removal of IL-2 by the FCS-CM-expanded cells as well as a direct inactivating effect on the CTL precursors (CTL-P) could be excluded as a reason for inhibition. On the other hand, we were able to show that the BM M phi population was very sensitive to the cytolytic activity exhibited by the inhibiting cells in a 3 h 51Cr-release assay and that the suppressor effect observed could be partially circumvented by a second addition of BM M phi on the second day after the initiation of the culture.
D000938:Antigen-Presenting Cells / Q000276:immunology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D008264:Macrophages / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D003470:Culture Media