Simplified microELISA for the quantitation of murine serum amyloid A protein.
A simplified microELISA has been developed which permits the rapid quantitation of serum amyloid A protein, SAA, in acute-phase mouse sera. Serum samples are directly diluted without prior denaturation into bicarbonate buffer and coated overnight onto microtiter plates. A rat polyclonal anti-SAA serum is used as the primary antibody followed by an alkaline phosphatase-conjugated goat anti-rat IgG serum. The specificity of the rat polyclonal serum has been demonstrated on immunoblots from high density lipoprotein preparations from normal and lipopolysaccharide induced acute-phase mouse sera. This assay permits the reproducible quantitation of murine SAA during an inflammatory response.
D000682:Amyloid / Q000032:analysis*; D000818:Animals; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D004731:Endotoxins / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D000685:Serum Amyloid A Protein / Q000032:analysis*
D000682:Amyloid; D004731:Endotoxins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D000685:Serum Amyloid A Protein