Selective killing of T lymphocytes by phototoxic liposomes.
Two-fold specificity in drug delivery obtained through the localized activation of drugs by physical means and the attachment of drugs to proteins that bind to target cells might be used for highly selective cancer chemotherapy or for immunosuppression. Toward this end, a monoclonal antibody against an antigen on the surface of T lymphocytes was covalently attached to liposomes containing a phototoxic drug, pyrene, bound to the lipid bilayer. When unfractionated peripheral blood lymphocytes, or B- and T-cell lines, were irradiated after treatment with these liposomes, T cells were killed while B cells were spared, demonstrating the validity of the approach in a simple in vitro assay.
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000166:cytology* / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival; D002784:Cholesterol; D004261:DNA Replication; D006801:Humans; D008027:Light; D008081:Liposomes; D008213:Lymphocyte Activation; D010713:Phosphatidylcholines; D011510:Proteolipids; D011721:Pyrenes / Q000633:toxicity*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000528:radiation effects
D000911:Antibodies, Monoclonal; D008081:Liposomes; D010713:Phosphatidylcholines; D011510:Proteolipids; D011721:Pyrenes; C020485:proteoliposomes; D002784:Cholesterol