Peptide and ester synthesis in organic solvents catalyzed by seryl proteases linked to alumina.
Trypsin and alpha-chymotrypsin were immobilized to alumina-phosphocolamine complex, activated by glutaraldehyde. The immobilized enzymes show a great stability toward organic solvents miscible or immiscible with water. In the presence of a low concentration of water, the immobilized enzymes catalyzed transesterification reactions as well as peptide synthesis. The synthesized peptides were stable toward the immobilized enzymes.
D000537:Aluminum Oxide; D002264:Carboxylic Acids; D002918:Chymotrypsin; D004800:Enzymes, Immobilized; D004951:Esterification; D004952:Esters; D010455:Peptides / Q000138:chemical synthesis*; D012697:Serine Endopeptidases; D012997:Solvents; D014357:Trypsin
D002264:Carboxylic Acids; D004800:Enzymes, Immobilized; D004952:Esters; D010455:Peptides; D012997:Solvents; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D000537:Aluminum Oxide