Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor.
A cDNA sequence coding for murine granulocyte colony-stimulating factor (G-CSF) has been isolated from a cDNA library prepared with mRNA derived from murine fibrosarcoma NFSA cells, which produce G-CSF constitutively. Identification of murine G-CSF cDNA was based on the cross-hybridization with human G-CSF cDNA under a low-stringency condition. The cDNA can encode a polypeptide consisting of a 30-amino acid signal sequence, followed by a mature G-CSF sequence of 178 amino acids with a calculated Mr of 19,061. The nucleotide sequence and the deduced amino acid sequence of murine G-CSF cDNA were 69.3% and 72.6% homologous, respectively, to the corresponding sequences of human G-CSF cDNA. The murine G-CSF cDNA, when introduced into monkey COS cells under the simian virus 40 promoter, could direct the synthesis of a protein that can stimulate the granulocyte colony formation from mouse bone marrow cells and support the proliferation of murine NFS-60 myeloid leukemia cells.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D006098:Granulocytes; D006801:Humans; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D003115:Colony-Stimulating Factors; D012333:RNA, Messenger; D004247:DNA