Macrophage colony-stimulating factor purified from normal human urine. Amino-terminal sequence and amino acid composition.
A macrophage colony-stimulating factor (M-CSF) was purified to homogeneity from a large amount of normal human urine. Microanalysis of the N-terminal amino acid sequence up to residue 44 revealed only a single residue difference from that deduced by other workers from the nucleotide sequence of M-CSF cDNA clones. The amino acid composition of the present preparation suggested that the M-CSF which we purified possessed a structure fitting the sequence 1-190 of TPA30-1 cell M-CSF deduced by Wong et al. [(1987) Science 235, 1504-1508].
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000652:urine*; D003115:Colony-Stimulating Factors / Q000652:urine*; D006801:Humans; D008262:Macrophage Activation; D008832:Microchemistry
D000596:Amino Acids; D003115:Colony-Stimulating Factors