Effects of recombinant human GM-CSF on proliferation of clonogenic cells in acute myeloblastic leukemia.
Proliferation of acute myeloblastic leukemia (AML) cells in vitro is limited in most cases to a small subset of blasts that have several properties of stem cells. These leukemic colony-forming cells (AML-CFU) generally require addition of exogenous growth factors for proliferation in agar or methylcellulose. These factors can be supplied by media conditioned by phytohemagglutinin-stimulated normal leukocytes or by CSF-secreting tumor cell lines. However, the exact factor or factors required for stimulation of AML-CFU growth have not been defined. We compared the AML-CFU stimulatory activity of a human recombinant GM-CSF with that of GCT-CM, Mo-CM, and the PHA-leukocyte feeder system in 15 cases of AML. In each of the 12 cases that required exogenous growth factors for maximum AML-CFU growth, recombinant GM-CSF could replace either GM-CSF or Mo-CM, and could partially replace the PHA-leukocyte feeder system. These results indicate that this GM-CSF is a growth promoter of AML-CFU in these culture systems.
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects*; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003470:Culture Media; D005260:Female; D006098:Granulocytes / Q000032:analysis; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology*
D003115:Colony-Stimulating Factors; D003470:Culture Media; D010835:Phytohemagglutinins; D011994:Recombinant Proteins