Lineage-related polypeptide markers in acute lymphoblastic leukemia detected by two-dimensional gel electrophoresis.
We have utilized two-dimensional polyacrylamide gel electrophoresis, coupled with ultrasensitive silver staining, to identify lineage-related polypeptide markers in lymphoblasts from children with acute lymphoblastic leukemia. Twelve polypeptides were detected that could distinguish between the major subgroups of acute lymphoblastic leukemia. These included a new marker for common acute lymphoblastic leukemia and markers for cells of B and T lineages. Analysis of the two-dimensional patterns also allowed the tentative identification of T-cell lineage in two cases with an otherwise undifferentiated non-T-cell non-B-cell phenotype. Two-dimensional electrophoresis thus provides a powerful tool for the delineation of the cell of origin in leukemia.
D001402:B-Lymphocytes / Q000378:metabolism; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000378:metabolism
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D010455:Peptides