A comparison of eosinophil-activating factor (EAF) with other monokines and lymphokines.
Monocytes from moderately eosinophilic individuals secrete material that enhances the cytotoxic activity of eosinophils against antibody-coated schistosomula of Schistosoma mansoni. This material is not a single substance, but can be fractionated into several active components of different size and different charge. Gel filtration of mononuclear cell supernatants separated the eosinophil-activating activity into a major component of molecular mass of 40 kDa and a minor component of molecular mass of less than 10 kDa. The major component exhibited further heterogeneity on fractionation by high performance liquid chromatography. The bulk of the eosinophil-activating activity could be separated from both colony-stimulating factor (CSF) alpha activity and from tumor necrosis factor (TNF) activity. However, human recombinant CSF alpha (GM-CSF), human recombinant TNF and rabbit tumor necrosis serum all had eosinophil-activating activity when tested against schistosomula. Eosinophils were not activated by interleukin 1, interleukin 2, interferon-alpha, lipopolysaccharide or phorbol myristate acetate.
D003115:Colony-Stimulating Factors / Q000494:pharmacology; D004804:Eosinophils / Q000187:drug effects*; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines / Q000302:isolation & purification / Q000494:pharmacology*; D009000:Monocytes / Q000032:analysis*; D015846:Monokines; D011506:Proteins / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha
D003115:Colony-Stimulating Factors; D006023:Glycoproteins; D007370:Interferon Type I; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D015846:Monokines; D011506:Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C022227:eosinophil stimulating promoter