The effects of tumor necrosis factor on the production of interleukin-1 by macrophages.
We investigated the effect of tumor necrosis factor (TNF) on interleukin-1 (IL-1) production by mouse macrophages. At high doses of TNF, macrophages produced no IL-1 (as judged in the LAF assay) even when exposed to bacterial lipopolysaccharide (LPS), an excellent inducer of IL-1 production. In lower (picogram/ml) doses, TNF caused macrophages to make factors which showed modest activity in the LAF assay and that activity could be blocked by antibody to IL-1. TNF induces in low doses also the production of a factor(s) which synergizes with IL-1 in the LAF assay.
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D004357:Drug Synergism; D006023:Glycoproteins / Q000378:metabolism / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha