Passive transfer of experimental allergic encephalomyelitis induced by proteolipid apoprotein.
In an attempt to obtain insight into the pathogenesis of proteolipid apoprotein (PLP)-induced experimental allergic encephalomyelitis (EAE) in Lewis rats (Yamamura et al. 1986), PLP-sensitized lymph node cells or spleen cells were passively transferred into normal or irradiated (400 rad) recipients after incubation with concanavalin A or PLP. Clinical EAE manifested by paraparesis was successfully transferred into irradiated recipients with 2 - 2.5 X 10(8) of the primary cultured cells and histologic EAE could be transferred with as few as 5 X 10(7) cells into naive recipients. This is the first demonstration of passive EAE induced with PLP-sensitized lymphoid cells and suggests the pathogenetic importance of cell-mediated immunity to PLP.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001059:Apoproteins / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D002490:Central Nervous System / Q000473:pathology; D003208:Concanavalin A / Q000494:pharmacology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D005260:Female; D007114:Immunization; D008213:Lymphocyte Activation / Q000187:drug effects; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D011510:Proteolipids / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew
D000906:Antibodies; D001059:Apoproteins; D011510:Proteolipids; D003208:Concanavalin A