Induction by recombinant human granulocyte colony-stimulating factor of differentiation of mouse myeloid leukemic M1 cells.
The effect of recombinant human granulocyte colony-stimulating factor (G-CSF) on induction of differentiation of mouse myeloid leukemic M1 cells was examined. Purified G-CSF caused dose-dependent induction of phagocytic activity and lysozyme activity in M1 cells. Its half-maximally effective concentration was 10 ng/ml. On treatment of M1 cells with G-CSF (100 ng/ml) for 4 days, 30-50% of the cells differentiated morphologically into macrophage cells; 30-40% of the cells were blast cells and 20-30% of the cells were forms intermediate between blastic cells and mature macrophages.
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D006023:Glycoproteins / Q000494:pharmacology; D006098:Granulocytes; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D007951:Leukemia, Myeloid / Q000473:pathology* / Q000503:physiopathology; D008222:Lymphokines; D008264:Macrophages / Q000473:pathology; D051379:Mice; D009113:Muramidase / Q000378:metabolism; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D006023:Glycoproteins; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D011994:Recombinant Proteins; D009113:Muramidase