Synthesis and release of deoxycytidine by human B and T lymphoblasts.
Deoxycytidine kinase activity is abundant in human T and B lymphocytes. However, the role of the enzyme in endogenous deoxynucleoside metabolism has not been established. The present experiments show that dividing human B lymphoblasts, but not T lymphoblasts, release substantial amounts of deoxycytidine (dCyd) into the medium, and have an active dCyd-dCMP (deoxycytidine-deoxycytidine 5'-phosphate) substrate cycle. Exogenous dCyd has been shown to protect human lymphocytes from the toxic effects of deoxyadenosine, deoxyguanosine and related compounds. Thus, the differential rates of dCyd release by T and B lymphocytes may affect the sensitivities of the two cell types to the growth inhibitory effects of exogenous deoxynucleosides.
D001402:B-Lymphocytes / Q000378:metabolism*; D002453:Cell Cycle; D002460:Cell Line; D003841:Deoxycytidine / Q000378:metabolism*; D003842:Deoxycytidine Kinase / Q000378:metabolism; D006801:Humans; D013601:T-Lymphocytes / Q000378:metabolism*; D013767:Tetrahydrouridine / Q000494:pharmacology
D003841:Deoxycytidine; D013767:Tetrahydrouridine; D003842:Deoxycytidine Kinase