Interleukin-3 production as a sensitive measure of T-lymphocyte activation in the mouse.
Interleukin-3 activity is produced by T cells at an early phase after antigenic stimulation, and its quantitation provides a sensitive and non-destructive method for assaying T-cell activation. It can be detected before increased uptake of [3H]thymidine into DNA occurs, and is more sensitive than measuring [3H]thymidine uptake for low levels of T-cell activation. This method has been shown to be useful in measuring activation of T-cell clones and in detecting antigens that have been blotted onto nitrocellulose.
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D002455:Cell Division; D005260:Female; D007377:Interleukin-3 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D012547:Schistosoma / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D000947:Antigens, Helminth; D007377:Interleukin-3; D013936:Thymidine