[Nuclei of lymphocytes of the T- and B-cell lines during normal embryogenesis and after treatment with hydrocortisone (morphometric and probabilistic-statistical parameters)].
Lymphoid cells of the thymus and of the Fabricius bursa have been studied in 18-day-old chick embryos, normal and after injection of hydrocortisone on the 11th day of embryogenesis. By means of optical-structural computer analysis, the complex of morphometric and probability-statistic parameters of the nuclei in the lymphocytes are estimated: area of the nuclei, optical density of chromatin, asymmetry coefficient and variance. Normal T-lymphocytes possess less density of the nuclei, greater optical density of chromatin, greater values of negative asymmetry. The complex of these parameters can be used for identification of visually similar lymphoid cells of T- and B-lines. Under hydrocortisone effect structural changes of the nuclei in the thymus and Fabricius bursa lymphocytes of the chick embryo are uniform: increase in the area of the nuclei, decrease in optical density of chromatin, the asymmetry coefficient becomes positive.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D002060:Bursa of Fabricius / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation / Q000187:drug effects; D002467:Cell Nucleus / Q000648:ultrastructure; D002642:Chick Embryo; D006854:Hydrocortisone / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology*
D006854:Hydrocortisone
Iadra limfotsitov T- i B-kletochnykh liniĭ v period émbriogeneza v norme i pri vozdeĭstvii gidrokortizonom (morfometricheskie i veroiatnostno-statisticheskie parametry).