Monoclonal antibodies in studying immunological phenotypes of neoplastic diseases.
Six hybridoma clones were obtained secreting monoclonal antibodies (Mab) against differentiating antigens of human hemopoietic cells. ICO-1 Mab detect Ia-like (Dr) antigens. Mab react with B-lymphocytes and monocytes without detecting antigens on granulocytes and T-cells. Antigen expression was enhanced following cell activation in a blast cell transformation test and mixed lymphocyte culture (MLC). ICO-1 Mab blocked MLC. The molecular weights of the antigen were 29 and 34 kilodaltons. Comparative studies of ICO-1 Mab with other Mab against Ia-like antigens revealed their identical reactivity. ICO-11 Mab detect the antigen on 4% of blood lymphocytes, 75% thymocytes, monoblasts, and CFU-GM. These Mab block NK-cell activity of blood mononuclear cells. ICO-GM-1 Mab detect the antigen on myelomonocytic cells and their precursors, but not CFU-GM. These Mab block binding of the C3Bi complement component to CR3 receptor and NK-cell activity. ICO-G2 Mab detect the antigen expressed at final stages of granulocyte differentiation. ICO-10 Mab detect the antigen on early thymocytes and ICO-02 on undifferentiated blast cells. Mab were shown to be applicable for human leukemia and lymphoma immune diagnosis.
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D002648:Child; D005260:Female; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D007938:Leukemia / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm