Inhibition of cell proliferation by interleukin-1 derived from monocytic leukemia cells.
Growth inhibitors and interleukin-1 (IL-1) are two biological response modifiers produced by mezerein-treated THP-1 cells maintained in serum-free medium. The activities comigrated with isoelectrofocusing in a pH range of 6.7 to 7.3. Subsequent molecular sieving on an AcA-54 column revealed that a portion of the growth-inhibitory activity for the mammary cell line MCF-7 remained associated with IL-1. IL-1-containing fractions were further analyzed by chromatography with DEAE-Sephacel, phenyl:Sepharose, and concanavalin A:Sepharose. In each instance, IL-1 coeluted with growth-inhibitory activity. IL-1 and growth-inhibitory activities partially purified by sequential isofocusing, AcA-54 chromatography, and DEAE-Sephacel were located in a single region following preparative polyacrylamide gel electrophoresis. Elution, concentration, and analytical polyacrylamide gel electrophoresis of this region resulted in a single band with an apparent molecular weight of 17,000. Stability studies revealed similarities between the IL-1 activity and growth-inhibitory activity in their sensitivity to a variety of physical and chemical treatments. A commercial source of human IL-1 also inhibited the growth of MCF-7 cells. DEAE-purified IL-1 derived from THP-1 cells inhibited the growth of 7 of 11 cell types tested, and all inhibited cell lines were established from malignant sources. Prostaglandin synthesis by MCF-7 cells in response to IL-1 was not responsible for growth inhibition.
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004224:Diterpenes; D006131:Growth Inhibitors / Q000302:isolation & purification*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007526:Isoelectric Point; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D008970:Molecular Weight; D011453:Prostaglandins / Q000502:physiology; D011489:Protein Denaturation; D013729:Terpenes / Q000494:pharmacology
D003470:Culture Media; D004224:Diterpenes; D006131:Growth Inhibitors; D007375:Interleukin-1; D011453:Prostaglandins; D013729:Terpenes; C011309:mezerein; D007213:Indomethacin