Increased yields of IL-2 in media conditioned by MLA 144 cells.
The gibbon leukaemia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 X 10(5) cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 X 10(-5) M and PMA at 10 ng/ml.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D006922:Hylobates; D007376:Interleukin-2 / Q000096:biosynthesis*; D007942:Leukemia, Experimental / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D007376:Interleukin-2; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate