The effect of aging on the expression of interleukin 2 messenger ribonucleic acid.
It is well documented that the production of interleukin 2 (IL-2) by lymphocytes declines with increasing age. Using a cDNA probe to IL-2, the effect of age on the expression of IL-2 mRNA was studied on concanavalin A-stimulated lymphocytes. The induction of IL-2 mRNA by concanavalin A was maximum after 20 hr of incubation. Northern blots demonstrated that the cDNA probe to IL-2 hybridized to an 11 S to 12 S RNA species. No age-related change in the size of the IL-2 mRNA was observed. In addition, there was no evidence of an age-related change in IL-2 mRNA degradation or the post-transcriptional processing of IL-2 mRNA. The induction of IL-2 mRNA decreased 85% between 5 and 29 months and paralleled the decline in IL-2 production and lymphocyte proliferation. Therefore, the age-related decline in the ability of lymphocyte population to produce IL-2 arises from a decrease in the ability of the lymphocytes to genetically express IL-2 mRNA.
D000375:Aging; D000818:Animals; D005786:Gene Expression Regulation; D007376:Interleukin-2 / Q000235:genetics*; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344
D007376:Interleukin-2; D012333:RNA, Messenger