Reversed-phase chromatography of interleukin-2 muteins.
Human recombinant interleukin-2 (IL-2) and related species have been characterized by chemical modifications, tryptic digestion, and cyanogen bromide digestion. The oxidation states of the cysteines and methionines in several IL-2 muteins have been determined. Reversed-phase high-performance liquid chromatography allowed us to distinguish the modifications in these muteins and to correlate retention behavior with their structure.
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003488:Cyanogen Bromide; D003545:Cysteine / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D007376:Interleukin-2 / Q000032:analysis*; D008715:Methionine / Q000032:analysis; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000032:analysis; D014357:Trypsin
D007376:Interleukin-2; D011994:Recombinant Proteins; D008715:Methionine; D014357:Trypsin; D003545:Cysteine; D003488:Cyanogen Bromide