Effect of chemical deglycosylation on the in vivo fate of ricin A-chain.
Chemical deglycosylation of ricin A-chain virtually eliminated its entrapment by the liver and delayed its clearance from the bloodstream of mice. Liver entrapment of native ricin A-chain occurred to approximately equal extents in the parenchymal and non-parenchymal cell fractions of the liver. The chemical deglycosylation procedure reduced uptake of the A-chain by both cell fractions.
D000818:Animals; D006031:Glycosylation; D000922:Immunotoxins / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008807:Mice, Inbred BALB C; D012276:Ricin / Q000378:metabolism*; D014018:Tissue Distribution
D000922:Immunotoxins; D012276:Ricin