Analysis of autoantibody reactivities in hybridoma collections derived from normal adult BALB/c mice.
Hybridoma collections were produced from lipopolysaccharide-stimulated adult BALB/c spleen cells, small resting spleen B cells and large activated spleen lymphocytes. The hybridomas were examined for production of immunoglobulins and of antibodies directed against a panel of self (actin, myosin, tubulin, DNA) and non-self antigens (myoglobin, spectrin, trinitrobenzene). From the 345 hybridomas secreting immunoglobulin, 68% did not react with any antigen of the panel, 17% reacted with only one, 5.5% with 2 and 7.9% with 3 or more. Apparently, each of the monoclonal multispecific antibodies exhibited a pattern of reactivity which was quite unique. There were no apparent differences in antibody reactivities between self and non-self antigens; moreover, with the exception of DNA, no differences were noted among the 3 different hybridoma collections.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000096:biosynthesis / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens