title: Procedure for establishing oligodendroglial cells in primary cultures based on developmental parameters.
abstract: We have established cultures using dissociated cells obtained from the neopallium of mouse pups at several different stages of development. The cellular composition of these cultures changed from more than 95% astrocytes when cultures were established from the neopallium of neonates to more than 90% oligodendrocytes when cultures were established from the neopallium of 2-week-old pups. The oligodendrocytic nature of the cells was established on morphological bases as well as the presence of an oligodendrocytic marker, galactocerebroside.
mesh_terms: D000367:Age Factors; D000818:Animals; D001253:Astrocytes / Q000032:analysis / Q000166:cytology / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000254:growth & development; D046508:Culture Techniques / Q000379:methods*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D051379:Mice; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000032:analysis / Q000166:cytology* / Q000502:physiology
chemical_list: D005904:Glial Fibrillary Acidic Protein