[Emergenec of low-mobility lymphocytes in the tumor-bearing status and its immunological significance].
The immunological significance of the lymphocyte mobility histogram derived from a fully automated cell electrophoretic instrument (Parmoquant-L), was considered. In mouse lymphocytes, the ratio of medullary thymocytes increased as a result of a decrease in the proportion of cortical thymocytes, whereas the ratio of splenocytes in the low-mobility zone (0.85-0.95 micron/sec/V/cm) increased in the course of tumor growth or in vivo concanavalin A-treatment. In the peripheral lymphocytes of cancer patients, the decreased ratio of high-mobility T cells resulted in a gradual increase in that of low-mobility T cells, which were not seen in lymphocytes modified by the patient's serum. Although the formation of blastoid cells by stimulation of the tumor or mitogen cannot be completely denied, the low-mobility T cells of peripheral lymphoid tissues in the tumor-bearing status are considered to be caused by incomplete differentiation of the cells, in which immunosuppressor cells are involved. Pattern analysis of lymphocyte electrophoresis would be a simple method if fully automated instrumentation was used.
D000818:Animals; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D004586:Electrophoresis; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D003208:Concanavalin A