An optimal assay of human B cell proliferation in vitro.
The purpose of this work was to optimize an in vitro assay for measuring B cell proliferation in response to S. aureus Cowan I (SAC). It has been demonstrated that the best proliferative activity is obtained when 10(5) cells are cultured in flat-bottomed microtiter plates in the presence of 1:10,000 SAC dilution. Standard RPMI 1640 culture medium should be enriched with mercaptoethanol and 10% FCS or 0.5% human albumin. Human serum, found to be an optimal supplement for T lymphocyte responses, blocks B cell proliferation.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008623:Mercaptoethanol / Q000494:pharmacology; D008722:Methods; D012709:Serum Albumin / Q000502:physiology; D013211:Staphylococcus aureus / Q000276:immunology
D003470:Culture Media; D012709:Serum Albumin; D008623:Mercaptoethanol