High-performance liquid chromatographic analysis of mefloquine and its main metabolite by direct plasma injection with pre-column enrichment and column switching techniques.
For the simultaneous determination of mefloquine and its main metabolite from plasma using high-performance liquid chromatography with pre-column enrichment and column switching techniques, two parameters proved crucial: use of phosphoric acid for acidification of plasma samples to ensure pre-column extraction of mefloquine and its main metabolite; careful observation of the pH of the eluent to ensure baseline separation of mefloquine and metabolite and separation from interfering plasma peaks. The described method employs direct plasma injection, is rapid, specific and sensitive, with an analysis time of 15 min and column lifetimes of ca. 200 injections.
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015767:Mefloquine; D011804:Quinolines / Q000097:blood* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D011804:Quinolines; D015767:Mefloquine