In vitro allostimulation of peripheral blood mononuclear cells generates autologous reactive T lymphocytes: analysis at the clonal level.
Cells from two siblings within family PER with a maternal crossing-over were used as responder and stimulator cells; they differed in class II MHC gene products. The responder cells were cloned and studied for their specificity. All the clones were T4+, most of them, were allospecific cytotoxic T-cell clones, some were only proliferative. Among the 62 clones that have been screened for their phenotype and function, we could expand, in our cloning system, two autoreactive T-cell clones specific for the self-class II MHC products. The results indicated that this autoreactivity was also observed in serum-free medium, which excludes any serum components susceptible to alter the self-class II MHC products. For the autorecognition, the two clones used the T3-Ti complex, and only one of them, the T4 structure.
D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D008297:Male; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007376:Interleukin-2; D013755:Tetradecanoylphorbol Acetate