Actions of prostaglandins E2 and F2 alpha on release of 14C-labelled mucins from rat submandibular salivary acini in vitro.
Mucin secretion was studied in vitro using well-characterized preparations of isolated acini. PGE2 significantly (p less than 0.05) increased release of [14C]-glucosamine labelled mucins at the highest concentration tested (10(-5) M), but was ineffective at lower doses (10(-9)-10(-6) M). PGF2 alpha had no effect on mucin secretion over this concentration range. PGE2 (10(-9)-10(-5) M) did not modify isoproterenol stimulated mucin secretion. The cyclooxygenase inhibitor indomethacin (10(-7)-10(-5) M) did not significantly inhibit either isoproterenol or noradrenaline stimulated mucin secretion. Thus it seems that, although PGE2 significantly increases mucin secretion at a high concentration, it is unlikely that prostaglandins play a major role in modulating beta-adrenergic stimulation of mucin secretion in rat submandibular acinar cells.
D000818:Animals; D002250:Carbon Radioisotopes; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009077:Mucins / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*
D002250:Carbon Radioisotopes; D009077:Mucins; D011458:Prostaglandins E; D011460:Prostaglandins F; D015237:Dinoprost; D015232:Dinoprostone; D007545:Isoproterenol; D009638:Norepinephrine; D007213:Indomethacin