title: Splenic migration of xid and non-xid splenic B cells.
abstract: The X-linked immune deficiency gene (xid) has been viewed as inducing either a deficiency in a B-cell subset or in B-cell maturation. The present experiments were performed in an attempt to better understand whether (a) xid B cells migrate differently from mature and immature non-xid B cells, and (b) whether the failure of mature B cells to be found in xid spleen, but not Peyer's patches, is secondary to migratory differences, especially the possible inability of xid spleens to receive mature B cells. We employed the technique of internally fluorescein labeling donor cells and subsequent injection into recipients. Double labeling permitted analysis of B or T cells by two-color flow microfluorometry. Functional studies revealed that labeled cells appropriately responded to TI-1 and TI-2 antigens after migration. We found that (a) adult xid splenic B cells do not migrate to spleen as well as adult non-xid splenic B cells, (b) the migration of adult xid splenic B cells to spleen resembles that of neonatal (xid and non-xid) B cells, (c) equal masses of xid and non-xid spleens have an equal capacity to receive either xid or non-xid splenic B cells, and (d) the migration of xid and non-xid T cells is similar. These results suggest that xid B cells do not migrate normally, but that migratory differences cannot account for the failure of mature B cells to be found in xid spleens.
mesh_terms: D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D002465:Cell Movement; D005260:Female; D007153:Immunologic Deficiency Syndromes / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains / Q000276:immunology*; D013154:Spleen / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation
chemical_list: D000955:Antigens, T-Independent