A new metabolic labelling medium for Trichomonas vaginalis and Tritrichomonas foetus using 35S methionine.
A metabolic labelling medium was devised for Trichomonas vaginalis and Tritrichomonas foetus utilizing 35S methionine. T. vaginalis cultured for 24h in the medium took up approximately 27% of the available label and increased greater than two fold in number. Counts per microgram of protein were 32,555 +/- 10% between different strains or identical strains in different labelling runs. T. foetus took up approximately 5% of the available label and increased greater than two fold in 24h. This resulted in specific labelling of 12,704 cpm/ug protein +/- 10% between different runs with the same strain.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005470:Fluorometry; D007553:Isotope Labeling / Q000379:methods*; D008715:Methionine / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014246:Trichomonas vaginalis / Q000378:metabolism*; D014318:Tritrichomonas / Q000378:metabolism*
D013462:Sulfur Radioisotopes; D008715:Methionine