Monkey interleukin 2: optimal conditions for production and partial characterization.
Cynomolgus monkey spleen cells and peripheral blood lymphocytes were tested for their ability to produce interleukin 2 (IL 2) upon stimulation with phytomitogens. By assessing proliferation of murine IL 2-dependent cells, maximal release of IL 2 activity was found in the supernatants of nonadherent cells cultured for 48 h, after stimulation with 25 micrograms/ml concanavalin A. Addition of phorbol myristate acetate to the cultures resulted in enhancement of IL 2 production, but there was no significant promotion when indomethacin or human lymphoblastoid cell lines were added to the cultures. Adherent macrophages appeared to inhibit rather than enhance IL 2 production in this system. Monkey IL 2 exhibited a molecular weight in the range of 15,000-18,000 daltons in Sephadex G-100 gel chromatography.
D000818:Animals; D003470:Culture Media; D005260:Female; D007376:Interleukin-2 / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D008222:Lymphokines / Q000494:pharmacology; D008251:Macaca / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D008934:Mitogens; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D007376:Interleukin-2; D008222:Lymphokines; D008934:Mitogens; D008623:Mercaptoethanol; D013755:Tetradecanoylphorbol Acetate