Photoaffinity labelling of androgen receptors from human foreskin and cultured genital fibroblasts with tritiated methyltrienolone [3H]R1881.
Androgen receptors were partially purified by affinity chromatography of cytosols prepared from either normal foreskins or normal cultured fibroblasts. Partially purified receptors were covalently labelled with [3H]R1881 (tritiated methyltrienolone) by ultraviolet photoactivation. Gel electrophoresis of cytosols from both sources showed two peaks of specific binding, Mr approximately 40 K and approximately 85 K, under denaturing conditions. Photoaffinity labelling of the human androgen receptor may thus provide a useful tool in further studies on disorders of androgenization.
D000345:Affinity Labels / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004963:Estrenes / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005837:Genitalia, Male / Q000378:metabolism*; D006801:Humans; D008297:Male; D015741:Metribolone; D008970:Molecular Weight; D010777:Photochemistry; D011944:Receptors, Androgen / Q000378:metabolism*
D000345:Affinity Labels; D004963:Estrenes; D011944:Receptors, Androgen; D015741:Metribolone