Catabolin/interleukin-1 regulation of cartilage and chondrocyte metabolism.
Catabolin/interleukin-1 effects on metabolism were studied in bovine nasal cartilage organ culture and articular chondrocyte cell culture. Keratan sulfate (KS) and hyaluronic acid (HA) were determined by an ELISA; prostaglandin E2 by RIA, sulfated glycosaminoglycan using dimethylmethylene blue and proliferation by incorporation of tritiated thymidine. Gel filtration of untreated 4-day organ culture media indicated that large sulfated and KS-containing proteoglycans were released and eluted in the void volume. Catabolin/interleukin-1 increased release of sulfated glycosaminoglycans and these were of lower molecular weight with an altered distribution of KS. Catabolin/interleukin-1 treatment of chondrocytes caused a decrease in KS production and proliferation but an increase in HA and in prostaglanding E2 production. Alterations of the chondrocyte metabolism by catabolin/interleukin-1 causing proteoglycan matrix degradation and modulation of chondrocyte glycosaminoglycan biosynthesis and proliferation may play a role in cartilage erosion and failure to repair in arthritic diseases.
D000818:Animals; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D046508:Culture Techniques; D015232:Dinoprostone; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology*; D053583:Interleukin-1beta; D007632:Keratan Sulfate / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis
D007375:Interleukin-1; D053583:Interleukin-1beta; D011458:Prostaglandins E; D006820:Hyaluronic Acid; D007632:Keratan Sulfate; D015232:Dinoprostone