Humoral immunity to dietary antigens in atopic dermatitis. I. Biotin-avidin amplified ELISA-analysis for serum IgG antibodies.
Enzyme-linked immunosorbent assays (ELISA) employing a biotin-avidin amplification step are described for the quantification of human serum IgG antibodies to the dietary antigens ovalbumin (OA) and beta-lactoglobulin (BLG). The analytical quality of these assays was acceptable. Antibodies were measured in 16 patients with mild or moderate atopic dermatitis (AD), in 31 patients with a history of AD, and in closely matched controls. Levels of serum anti-OA antibodies did not differ in patients and controls, whereas anti-BLG antibodies tended to be higher in patients with mild or moderate AD than in controls (P less than 0.05).
D000293:Adolescent; D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis* / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000008:administration & dosage; D001360:Avidin / Q000494:pharmacology; D001710:Biotin / Q000494:pharmacology; D002648:Child; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology*; D004032:Diet; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007074:Immunoglobulin G; D007782:Lactoglobulins; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin