Augmentation of hemolytic efficiency of anti-hapten IgG antibody by anti-IgG antibody is primarily the function of the anti-hapten IgG.
IgG anti-cell antibodies are inefficient in inducing cell lysis by complement. C mediated lysis by anti-cell IgG can be augmented by the use of C fixing anti-antibody. We have studied augmentation of rabbit anti-Forssman IgG, rabbit anti-methotrexate IgG and rabbit anti-folinic acid IgG antibodies by rabbit anti-allotype IgG antibodies. Large variability (from a low of 2 to a high of over 100) was found in augmentation efficiency even with the same anti-allotype antibody; the variability was primarily the function of the anti-hapten antibody. It was concluded that quantitative studies on anti-hapten IgG production relying on augmented hemolysis may lead to misleading conclusions.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology; D005577:Forssman Antigen / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D006461:Hemolysis; D007074:Immunoglobulin G / Q000276:immunology*; D002955:Leucovorin / Q000276:immunology; D008727:Methotrexate / Q000276:immunology; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D006241:Haptens; D007074:Immunoglobulin G; D003165:Complement System Proteins; D005577:Forssman Antigen; D002955:Leucovorin; D008727:Methotrexate