Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus. V. Recognition is restricted to gene products encoded by the viral S RNA segment.
Lymphocytic choriomeningitis virus (LCMV) Armstrong (ARM) strain-specific, H-2d-restricted CTL effectively lyse syngeneic targets infected by LCMV ARM, but show reduced killing of LCMV Pasteur (PAST) strain-infected H-2d cells. We have reassorted the two RNA segments, large (L) and small (S), of LCMV ARM and PAST to generate LCMV with genotypes of L ARM/S PAST and L PAST/S ARM. By using these reassortants and both LCMV primary CTL and CTL clones, we report that the induction, recognition, and lysis of LCMV-specific CTL depend on the S RNA segment and the genes it encodes.
D000818:Animals; D002999:Clone Cells / Q000145:classification / Q000276:immunology / Q000382:microbiology; D003602:Cytotoxicity, Immunologic; D005814:Genes, Viral; D005838:Genotype; D006183:H-2 Antigens / Q000235:genetics; D008213:Lymphocyte Activation; D008216:Lymphocytic Choriomeningitis / Q000276:immunology; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014176:Protein Biosynthesis; D012342:RNA, Small Nuclear / Q000235:genetics*; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology* / Q000382:microbiology
D006183:H-2 Antigens; D012342:RNA, Small Nuclear