Determination of the (+)- and (-)-enantiomers of pirprofen in human plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method was developed to determine the (+)- and (-)-enantiomers of pirprofen, an anti-inflammatory drug. After addition of an internal standard, the plasma sample was brought onto a glass column pre-packed with silica and eluted with dichloromethane. The extracts were derivatized with 1,1'-carbonyldiimidazole and R (+)-1-methylbenzylamine to form the two diastereomeric amides. The diastereoisomers were separated on a chiral column by HPLC with ultraviolet detection at 272 nm using n-hexane-dichloromethane (64:36, v/v) as the mobile phase. The limit of quantitation was 0.992 mumol/l (0.25 microgram/ml) for each enantiomer.
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006801:Humans; D010666:Phenylpropionates / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010666:Phenylpropionates; C008924:pirprofen