Membrane-associated interleukin 1 activity on human U937 tumor cells: stimulation of PGE2 production by human chondrosarcoma cells.
Membrane-associated interleukin 1 (IL 1) activity was induced on the human macrophage tumor cell line, U937, by pretreatment with phorbol myristic acid (PMA). Incubation of PMA-treated, paraformaldehyde-fixed U937 cells with the murine cell line D10.G4.1 in the presence of concanavalin A caused an increase in DNA synthesis as measured by the uptake of tritiated thymidine. Paraformaldehyde-fixed U937, not pretreated with PMA, showed little or no activity. A rabbit polyclonal antibody directed against human IL 1 neutralized all membrane-associated IL 1-like activity, as measured by the inhibition of D10.G4.1 cell proliferation. PMA-treated U937 caused a pronounced enhancement of PGE2 production from a human chondrosarcoma cell line, SW-1353. Membrane-associated IL 1 induced a more potent PGE2 response than did a maximal concentration of soluble IL 1. Rabbit antihuman IL 1 neutralized membrane-bound IL 1 induction of PGE2. The data presented here raise the possibility that membrane-bound IL 1 may play a primary role in the pathophysiology of the inflammatory disease process.
D002462:Cell Membrane / Q000502:physiology; D002813:Chondrosarcoma / Q000503:physiopathology*; D015232:Dinoprostone; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology; D011458:Prostaglandins E / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000166:cytology
D007375:Interleukin-1; D008565:Membrane Proteins; D011458:Prostaglandins E; D015232:Dinoprostone