Transient induction of photolyase activity in arrested frog cells in response to a short-wave ultraviolet segment of simulated "sunlight".
Induction of photolyase activity was studied in cultured frog cells using clonogenic assays. Exposure of arrested cells to a pre-irradiation (90% survival) of 254 nm ultraviolet light resulted in a transient enhancement of photolyase activity. Cells expressed a decreased level of photolyase activity in response to an equitoxic fluence of simulated "sunlight" wavelengths 280-310 nm. However, no significant increase of enzyme activity was detected in cells following treatment with "sunlight" wavelengths 310-330 nm. In addition, this process depends on newly biosynthesized protein(s).
D000818:Animals; D002460:Cell Line; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D007700:Kinetics; D008190:Lyases / Q000528:radiation effects*; D011894:Rana pipiens; D014466:Ultraviolet Rays
D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase