title: Activated T-cells and macrophages in the cerebrospinal fluid and the spinal meningeal exudate in chronic relapsing experimental allergic encephalomyelitis.
abstract: An analysis has been made of the cell types which mark with monoclonal antibodies against T cells, macrophages and the IL-2 receptor (anti-Tac) in the blood, cerebrospinal fluid (CSF) and spinal meningeal exudates taken from guinea pigs in the relapse and remission stages of chronic relapsing experimental allergic encephalomyelitis (CR-EAE). Whilst the T-cell and macrophage content of blood remained unchanged throughout the course of CR-EAE, T cells accounted for the majority of the CSF pleocytosis associated with relapsing disease but both T cells and macrophages populated the meningeal exudate in substantial numbers. Activated T cells (Tac+) rose in number in blood only after the onset of relapse but formed a far higher proportion of the CSF pleocytosis or meningeal exudate than in paired blood samples. Meningeal exudate cells from Freund's adjuvant-inoculated, but not uninoculated animals, also showed an increase in Tac+ cell levels. In addition, the meningeal exudate contained a substantial number of cells which did not label with anti-T or anti-macrophage antibodies and which did not vary in absolute numbers throughout the course of disease.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002452:Cell Count; D002555:Cerebrospinal Fluid / Q000166:cytology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D005122:Exudates and Transudates / Q000276:immunology; D006168:Guinea Pigs; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008578:Meninges / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000954:Antigens, Surface