[Nonstructural glycosylated polyproteins coded for by the gag gene of retrovirus type D expressed on the plasma membrane].
Studies of virus-specific proteins of type D retroviruses by radioimmunoprecipitation (RIP) using various radioactive precursors demonstrated in HEp-2 cells producing these retroviruses the presence of two glycosylated gag-coded polyproteins with molecular weights of 78 K and 90 K. The same polyproteins were found by lactoperoxidase iodination and RIP on the plasma membrane of the cells. Comparative studies of these glycoproteins by the limited proteolysis method showed the sites for trypsin action in them to be located in similar places of protein bases which indicated their structural relationship. It is suggested that glycosylated gag-coded polyproteins of retroviruses (including type D retroviruses) play the role of receptors for certain mitogens the seizure of which stimulates cell proliferation.
D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D015683:Gene Products, gag; D005814:Genes, Viral; D005815:Genetic Code; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D008970:Molecular Weight; D011863:Radioimmunoassay; D012190:Retroviridae / Q000235:genetics*; D012191:Retroviridae Proteins / Q000032:analysis / Q000235:genetics*; D013050:Spectrometry, Fluorescence
D015683:Gene Products, gag; D008565:Membrane Proteins; D012191:Retroviridae Proteins
Nestrukturnye glikozilirovannye poliproteiny, kodiruemye genom gag retrovirusa tipa D, ékspressirovany na plazmaticheskoĭ membrane.