Bone-derived and recombinant transforming growth factor beta's are potent inhibitors of tumor cell growth.
Two naturally occurring chrondogenesis inducing peptides have been purified to homogeneity from demineralized bovine bone. Cartilage-inducing factors A and B are the bone-derived equivalents of transforming growth factor-beta types I and II. Both peptides exhibit identical biological activities in chondrogenesis assays and stimulate anchorage independent cell growth. In this study we show that both bone-derived factors are potent (ng/ml) inhibitors of both DNA synthesis and the anchorage independent growth of a variety of human and non-human tumor cells. Unique in this study is also a comparison of the activities of these polypeptide growth factors with recombinant transforming growth factor type I expressed in mammalian cells.
D000818:Animals; D001842:Bone and Bones / Q000032:analysis*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D015291:Transforming Growth Factors
D006133:Growth Substances; D010455:Peptides; D011994:Recombinant Proteins; D015291:Transforming Growth Factors