Immune abnormalities associated with HLA-B8: lymphocyte subsets and functional correlates.
Mononuclear cell populations were enumerated in healthy young adults with or without the histocompatibility antigen HLA-B8. Mononuclear cell counts were lower in subjects with HLA-B8, as was reflected in lower absolute numbers of the cell subsets. When cell populations were compared as percentages of total mononuclear cells, subjects with HLA-B8 had significantly more B lymphocytes bearing IgM than did subjects without HLA-B8. The T4/T8 ratio was significantly increased in subjects with HLA-B8. due both to increases in OKT4+ cells and to decrease in OKT8+ cells. B-Lymphocyte function was compared in subjects with and without HLA-B8 by measuring pokeweed mitogen driven differentiation of B cells to IgM bearing blasts, and was significantly greater in subjects with HLA-B8. Subjects with HLA-B8. Subjects with HLA-B8 were also found to have decreased Con A-induced suppression. Alterations in lymphocyte subsets and lymphocyte functions may underlie the predisposition to autoimmunity associated with HLA-B8.
D000328:Adult; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D005434:Flow Cytometry; D006680:HLA Antigens / Q000032:analysis*; D015795:HLA-B8 Antigen; D015803:HLA-DR3 Antigen; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D012016:Reference Values; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015795:HLA-B8 Antigen; D015803:HLA-DR3 Antigen; D000949:Histocompatibility Antigens Class II; D007075:Immunoglobulin M