Thyroid hormones alter mRNA activity profiles of differentiated 3T3-L1-cells.
The influence of thyroid hormones on the gene expression of 3T3-L1-adipocytes was examined. mRNA activity profiles were investigated by in vitro-translation and 2D-electrophoresis. Seven protein spots out of 210 were sensitive to 3,5,3'-triiodothyronine (T3). All proteins increased from hypothyroid to hyperthyroid status. Additionally two spots increased from hypothyroid to T3-substituted and euthyroid status. There is evidence for the existence of protein S14 in differentiated 3T3-L1-cells, as part of spot 5.
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D051379:Mice; D009687:Nuclear Proteins; D014176:Protein Biosynthesis; D011506:Proteins; D012333:RNA, Messenger / Q000096:biosynthesis*; D013268:Stimulation, Chemical; D014157:Transcription Factors; D014284:Triiodothyronine / Q000494:pharmacology*
D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; C051065:THRSP protein, human; C501477:Thrsp protein, mouse; D014157:Transcription Factors; D014284:Triiodothyronine