Characterization of a beta-lactamase from Pasteurella multocida.
A bovine strain of Pasteurella multocida was found to produce a beta-lactamase. The minimal inhibitory concentrations of amoxicillin (128 mg/l) and ticarcillin (512 mg/l) were high for this strain. The enzyme was periplasmic and produced constitutively, and there was no obvious permeability barrier. This enzyme had a TEM-substrate profile, and no inhibition was detected with antisera (anti-TEM-1, anti-TEM-2). The apparent isoelectric point was estimated at 8.8.
D000658:Amoxicillin / Q000494:pharmacology; D000667:Ampicillin / Q000494:pharmacology; D000668:Ampicillin Resistance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D004352:Drug Resistance, Microbial; D007525:Isoelectric Focusing; D008168:Lung / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D010325:Pasteurella / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D010403:Penicillin Resistance; D013982:Ticarcillin / Q000494:pharmacology; D001618:beta-Lactamases / Q000032:analysis* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D000667:Ampicillin; D000658:Amoxicillin; D001618:beta-Lactamases; D013982:Ticarcillin