A simple method to deplete human interleukin 2-containing medium of phytohemagglutinin by filtration through a concanavalin A sepharose 4B column.
A method is presented to deplete human interleukin 2 (IL-2) containing conditioned medium of phytohemagglutinin (PHA) by filtration through a concanavalin A Sepharose 4B (Con A Seph 4B) column. The presence of residual PHA was determined by measuring the mitogenic effect of treated medium on freshly isolated blood mononuclear cells as well as by counter-immunoelectrophoresis using an anti-PHA serum. A one-step passage over a Con A Seph 4B column removes 80% or more of the PHA present in conditioned medium without a significant loss of IL-2 activity. These data show that the use of a Con A Seph 4B column is a simple, fast and economical method for the preparation of IL-2-containing medium for use on long-term human T cell cultures.
D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D003208:Concanavalin A; D003378:Counterimmunoelectrophoresis; D003470:Culture Media; D005374:Filtration; D006801:Humans; D007376:Interleukin-2 / Q000302:isolation & purification*; D010835:Phytohemagglutinins / Q000302:isolation & purification*
D003470:Culture Media; D007376:Interleukin-2; D010835:Phytohemagglutinins; D003208:Concanavalin A