title: Cellular immunity to collagen in periodontal disease: role of T, B lymphocytes and adherent cells.
abstract: Peripheral blood lymphocytes of patients with periodontal disease and control subjects were fractionated into T enriched and B enriched lymphocyte populations and plastic adherent cells (PACs). T enriched and B enriched lymphocytes were cultured: (a) with human Type I collagen with varying concentrations of PACs and (b) without collagen, but with cells or supernatants from autologous and heterologous PACs which had been previously cultured with and without collagen. In patients with periodontal disease, both T enriched and B enriched blastogenic responses were higher than in control subjects, but B enriched cell responses of patients were highest. The addition of PACs had no significant effect upon the enriched T cell responses to collagen; the B enriched cell responses were enhanced in the presence of PACs, the maximum response occurring with the addition of approximately 10% PACs. T enriched cells did not appear to respond to the collagen co-cultured PACs or PAC supernatants; B enriched cells responded maximally to PACs co-cultured with collagen. Autologous PACs, co-cultured with collagen, induced higher responses than heterologous PACs similarly treated.
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D003094:Collagen / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D008297:Male; D010510:Periodontal Diseases / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D003094:Collagen