Isolation of a human von Willebrand factor cDNA from the hybrid endothelial cell line EA.hy926.
A permanent human hybrid endothelial cell line (EA.hy926) was shown to produce the von Willebrand factor, a protein of 250,000 relative mass (Mr) which was secreted into the medium as a 220,000 Mr protein. A cDNA library was constructed in lambda gt11 using mRNA from these hybrid cells. Several von Willebrand factor cDNA clones were isolated from this library using a synthetic oligodeoxyribonucleotide as a hybridization probe. These cDNA clones were used to analyze the von Willebrand factor gene in normal individuals and in cultured cells.
D002460:Cell Line; D004247:DNA / Q000302:isolation & purification*; D004261:DNA Replication; D004727:Endothelium / Q000378:metabolism; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D014471:Umbilical Veins; D014841:von Willebrand Factor / Q000235:genetics*
D014841:von Willebrand Factor; D004247:DNA