Accessory cell function of cells isolated from Mycobacterium leprae-induced granulomas.
The large cells from Mycobacterium leprae-induced granulomas in guinea pig lymph nodes were separated by Percoll discontinuous density gradient centrifugation and on a fluorescence-activated cell sorter (FACS) using cross-reacting monoclonal antibody to human MHC Class II antigens. Large Percoll-separated cells (83% Class II antigen positive and 52% macrophage-specific antigen positive) and FACS-separated cells are able to act as antigen-presenting cells for T-cell proliferation to PPD. In previous studies, macrophage antigen-positive cells consistently failed to act as accessory cells. This indicates that there is a population of accessory cells which are macrophage antigen negative and MHC Class II antigen positive present in these M. leprae-induced granulomas.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D006099:Granuloma / Q000276:immunology* / Q000473:pathology; D006168:Guinea Pigs; D006681:HLA-D Antigens / Q000032:analysis; D007918:Leprosy / Q000276:immunology* / Q000473:pathology; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
D006681:HLA-D Antigens