Stimulation of B cells by sequential addition of anti-immunoglobulin antibody and cytochalasin.
B cells are stimulated to initiate DNA synthesis by modest doses of anti-immunoglobulin antibody in combination with cytochalasin. The ability of these agents to stimulate B cells in a sequential fashion was evaluated. Anti-immunoglobulin prepared cells to respond to subsequently added cytochalasin, but cytochalasin did not prepare cells to respond to anti-immunoglobulin. Only brief exposure to anti-immunoglobulin was needed to activate B cells for cytochalasin responsiveness; once activated, B cells remained responsive to cytochalasin for hours. The ability of cytochalasin to supply a second signal to activated B cells suggests that the cytoskeleton may be involved in generating or transducing growth-promoting signals for B lymphocytes.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D006041:Goats; D006133:Growth Substances / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008805:Mice, Inbred A; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D003572:Cytochalasins; D006133:Growth Substances; D015638:Cytochalasin D; D004247:DNA