Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells.
A rise of cytosolic Ca2+ is induced by NGF in rat pheochromocytoma PC12 and bovine chromaffin cells investigated (both in suspension and while attached to polyornithine-coated glass slides) by fluorescence techniques (with quin-2 and fura-2). The effect of NGF on [Ca2+]i is delayed (30-40 s of lag phase), slow (t1/2 = 40 s), relatively small (+50-75%) and persistent (over 10 min). It is due to Ca2+ influx (requires extracellular Ca2+ greater than 10 microM) through a pathway different from the voltage-gated Ca2+ channel, possibly accompanied by intracellular Ca2+ redistribution, and might play a messenger role in NGF action.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000634:Aminoquinolines; D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002838:Chromaffin System / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000378:metabolism; D051381:Rats
D000634:Aminoquinolines; D001572:Benzofurans; D005456:Fluorescent Dyes; D009414:Nerve Growth Factors; D004815:Epidermal Growth Factor; C035739:Quin2; D002118:Calcium; D016257:Fura-2