Molecular cloning of a prolactin-related mRNA expressed in bovine placenta.
Bovine (Bos taurus) prolactin-related cDNA I (bPRC-I), distinct from the isolated bovine placental lactogen, was derived from bovine fetal placental mRNA by molecular cloning. The nucleotide sequence is 63% homologous to bovine prolactin cDNA and only 45% to bovine growth hormone. The region of bPRC-I corresponding to the 5' portion of the signal peptide and 5' untranslated region of bovine prolactin mRNA is markedly different from prolactin. The predicted protein is 39% homologous to bovine prolactin and about 30% to the related placental hormones in rodents. This identification of a prolactin-related gene in the cow in addition to those reported in rodents suggests that multiple prolactin-related genes expressed in the placenta may be a general phenomenon in nonprimates. The role of these related hormones during gestation remains to be investigated.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D013006:Growth Hormone / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000032:analysis*; D010928:Placental Lactogen / Q000235:genetics; D011388:Prolactin / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*
D012333:RNA, Messenger; D011388:Prolactin; D013006:Growth Hormone; D004247:DNA; D010928:Placental Lactogen