[Preliminary results of the reactivity of T lymphocytes with HIV- capsid-antibodies].
HIV-infected and noninfected H9-cell cultures were incubated with two antibodies (Biochrom, DuPont) against p24gag antigen (capsid) of HIV: the antibodies show a positive reaction against p24 antigen in the Western blot. The antibody-labeled cells were examined by fluorescence-activated cell sorting (FACS) and by microscope. The labeled infected and uninfected cells show no characteristic fluorescence pattern after previous acetone fixation. By using formalinfixation technique the monoclonal antibody (DuPont) shows a positive fluorescence pattern but also a high unspecific reactivity with uninfected H9-cells. An identification of HIV-infected living cells with this monoclonal capsid antibody seems possible, if the unspecific binding can be reduced.
D000914:Antibodies, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology; D002478:Cells, Cultured; D006678:HIV / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000914:Antibodies, Viral
Voruntersuchungen zur Reaktivität von T-Lymphozyten mit HIV-Kapsid-Antikörpern.