title: Modification of the platelet-binding domain of von Willebrand factor.
abstract: Radioiodinated Bolton-Hunter reagent was used at low specific activity to probe for the function and reactivity of amino groups on von Willebrand factor (vWF), a plasma protein involved in platelet responses to damaged endothelial surfaces. The platelet receptor for vWF contains a membrane protein termed glycoprotein Ib. Modification of only one or two amino groups per vWF subunit caused a 50% reduction in the platelet-agglutinating activity of vWF, and a decrease in its ability to bind to platelets. All multimeric forms of vWF are modified. Loss of platelet-agglutinating activity on modification of less than 2% of the amino groups on each vWF subunit suggests that the amino groups in the glycoprotein Ib-binding domain of vWF are both particularly reactive and essential for its function.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D014841:von Willebrand Factor