Simple rosette assay for HLA-B27 typing of whole blood samples.
Two human IgM monoclonal antibodies (TrBH12 and Tr3B6) specific for HLA-B27 were coupled to magnetic monodisperse polymer beads by spontaneous adsorption. A few minutes after mixing the beads with 50 microliter of whole blood, rosettes had formed specifically with leucocytes from HLA-B27 positive individuals. In this simple way, reliable typing for HLA-B27 could be conducted rapidly (in about 20 min) by evaluation of rosettes with an inverted microscope and ordinary light. The method requires purified monoclonal antibodies.
D000911:Antibodies, Monoclonal; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D015796:HLA-B27 Antigen; D006801:Humans; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015796:HLA-B27 Antigen