[Lines of transformed mouse thymus cells. II. Cell morphology, karyology, ultrastructure and growth in vitro and in vivo].
Morphology, fine structure, karyology and growth of intrathymus pre-T-cell cultures (TC.SC-1/1.1 and TC.SC-1/2.0) were studied both in vitro and in vivo. The cultures were induced by injecting to mice a supernatant enriched with interleukin 2. The results obtained confirm the malignant transformation of cells of the lines obtained and the involvement of endogenic lymphotropic viruses in this process. The lines obtained are defective in hypoxanthine phosphoribosyltransferase. This property may serve as a basis for their use in hybridoma technology.
D000818:Animals; D002455:Cell Division; D002461:Cell Line, Transformed; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency; D066298:In Vitro Techniques; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D013601:T-Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000637:transplantation; D013950:Thymus Gland / Q000166:cytology* / Q000201:enzymology / Q000637:transplantation; D014185:Transplantation, Isogeneic; D014407:Tumor Cells, Cultured
D007041:Hypoxanthine Phosphoribosyltransferase
Linii transformirovannykh kletok timusa myshi. II. Morfologiia, kariologiia, ul'trastruktura, rost kletok in vitro i in vivo.