A conjugate of trypsin and chymotrypsin.
A heteroenzyme conjugate retaining activities of two component enzymes from trypsin and chymotrypsin was prepared using N-succinimidyl pyridyl dithiopropionate as crosslinking reagent. The conjugate bound to both trypsin and chymotrypsin affinity columns. Trypsin and chymotrypsin were linked in the ratio of 1:1 on mol basis. The conjugate, when treated with dimethyladipimidate, showed decreased autolysis of its trypsin component.
D002918:Chymotrypsin / Q000032:analysis / Q000378:metabolism*; D003432:Cross-Linking Reagents; D009097:Multienzyme Complexes / Q000138:chemical synthesis*; D011506:Proteins / Q000032:analysis; D013388:Succinimides; D014357:Trypsin / Q000032:analysis / Q000378:metabolism*
D003432:Cross-Linking Reagents; D009097:Multienzyme Complexes; D011506:Proteins; D013388:Succinimides; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate; D002918:Chymotrypsin; D014357:Trypsin