title: Necator americanus in neonatally infected hamsters. The time-course of infection and antibody response to the surface antigens of L4 and adult worms.
abstract: Neonatal hamsters were infected with a hamster-adapted strain of Necator americanus, and the time-course of infection was followed by worm and faecal egg counts. Parasite eggs were first recorded during the 6th week of infection, increasing rapidly thereafter to peak in weeks 7-10. Male hamsters excreted more eggs than females, but both sexes were equally susceptible to infection and harboured comparable worm burdens. Faecal egg counts declined from week 10 onwards and this was associated with a loss of worms from animals with heavy infections. Low level infections were stable over the first 114 d of infection but worm fecundity nevertheless still declined over this period. Both hamster sexes responded similarly to surface antigens on adult worms, the antibody levels rising from week 5 onwards to reach a plateau in weeks 6-7, which persisted until the experiments were terminated. The major antigens recognised on the surface of adult worms had molecular masses corresponding to 25 kDa, 32 kDa, a doublet with the heaviest polypeptide resolving at 46 kDa, and a triplet with the heaviest at 67 and 93kDa. In contrast L4 had only 2 major cuticular antigens resolving at 41 and 93kDa. The 93kDa molecule on L4 and adult worms may be antigenically related.
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D006224:Cricetinae; D005243:Feces / Q000469:parasitology; D005260:Female; D008297:Male; D009331:Necator / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D009332:Necatoriasis / Q000276:immunology* / Q000469:parasitology; D012737:Sex Factors; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D000954:Antigens, Surface