Comparative efficacy of broad-spectrum antiviral agents as inhibitors of African swine fever virus replication in vitro.
Various nucleoside analogues, selected on the basis of their previously established broad-spectrum antiviral properties, were evaluated for their potency and selectivity as inhibitors of the in vitro replication of the iridovirus, African swine fever virus (ASFV). The test compounds included (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(S)-HPMPA], 9-(2-phosphonylmethoxyethyl)adenine, (RS)-3-adenin-9-yl-2-hydroxypropanoic acid isobutyl ester, (S)-9-(2,3-dihydroxypropyl)adenine, carbocyclic 3-deazaadenosine (C-c3Ado), 3'-azido-2',3'-dideoxythymidine, pyrazofurin and ribavirin. As the most efficacious inhibitors of ASFV replication emerged (S)-HPMPA followed by C-c3Ado. The minimum inhibitory concentration of (S)-HPMPA for ASFV replication was 0.01 microgram/ml, and its selectivity index was 15,000. The corresponding values for C-c3Ado were 0.025 micrograms/ml and 8000, respectively. It would seem justified to further pursue these compounds for their anti ASFV activity in vivo.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000358:African Swine Fever Virus / Q000187:drug effects* / Q000502:physiology; D000577:Amides; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007497:Iridoviridae / Q000187:drug effects*; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D011720:Pyrazoles; D012254:Ribavirin / Q000494:pharmacology; D012263:Ribonucleosides / Q000494:pharmacology; D012266:Ribose; D013936:Thymidine / Q000031:analogs & derivatives / Q000494:pharmacology; D014372:Tubercidin / Q000031:analogs & derivatives / Q000494:pharmacology; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine
D000577:Amides; D000998:Antiviral Agents; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D011720:Pyrazoles; D012263:Ribonucleosides; D012254:Ribavirin; C002997:pyrazofurin; D015215:Zidovudine; C016391:9-(2,3-dihydroxypropyl)adenine; D012266:Ribose; C053001:adefovir; C034072:carbocyclic 3-deazaadenosine; C048336:3-adenin-9-yl-2-hydroxypropanoic acid isobutyl ester; C050127:9-(S)-(3-hydroxy-2-(phosphonomethoxy)propyl)adenine; D000225:Adenine; D014372:Tubercidin; D013936:Thymidine