Phenytoin potentiates accessory cell dependent DNA-synthesis in human lymphocytes in vitro.
The effect of phenytoin and its major metabolite p-HPPH on concanavalin A (ConA) induced DNA-synthesis of human lymphocytes was studied in vitro. In lymphocyte cultures depleted of phagocytizing cells by iron treatment PHT and p-HPPH, in pharmacologic concentrations, caused a depression of the mitogen response measured as uptake of 3H-thymidine. In contrast, the presence of phagocytizing mononuclear cells during ConA-stimulation in the presence of PHT and p-HPPH caused a potentiation of ConA induced DNA-synthesis. These effects of phenytoin on immunocompetent cells may be of significance in the pathogenesis of the PHT-induced gingival overgrowth, since earlier we have reported the presence of large infiltrates of lymphocytes, mainly T-lymphocytes in gingival biopsies from clinically non-inflamed PHT-induced gingival overgrowth.
D000328:Adult; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D010672:Phenytoin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
D007376:Interleukin-2; D003208:Concanavalin A; C027364:5-(3-hydroxyphenyl)-5-phenylhydantoin; D010672:Phenytoin; D004247:DNA