Gastric mucosa protective effects of colloidal bismuth subcitrate (DE-NOL).
We evaluated the gastric mucosal protective properties of the anti-ulcer drug, colloidal bismuth subcitrate (CBS; DE-NOL), and its ability to stimulate mucosal synthesis of PGE2 in the rat. Gastric lesions were induced by ethanol and quantified by a visual scoring procedure. CBS was about 3-4 times more protective than sucralfate at reducing lesions. PGE2 displayed potent activity in this model, though cimetidine displayed only weak activity. Increasing the concentration of a standard dose of CBS in the rat stomach enhanced the protective activity against ethanol lesions. Pretreatment of rats with CBS led to complete, partial and no protection at 0.25, 8 and 16 h respectively. PGE2 generation in gastric mucosa biopsies was dose-dependently increased by oral CBS and peak synthesis occurred at 0.25 h. Although partial protection against ethanol lesions was found 8 h after CBS, basal levels of PGE2 generation had already returned at 4 h. Indomethacin blocked CBS-stimulated generation of PGE2, but only partially blocked the protection against ethanol-induced lesions. These findings indicated that CBS protects the rat gastric mucosa against ethanol lesions and both prostaglandin- and non-prostaglandin-mediated mechanisms could contribute to this protection.
D000818:Animals; D000897:Anti-Ulcer Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D015232:Dinoprostone; D000431:Ethanol / Q000037:antagonists & inhibitors; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009942:Organometallic Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011458:Prostaglandins E / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control; D013392:Sucralfate / Q000494:pharmacology
D000897:Anti-Ulcer Agents; D009942:Organometallic Compounds; D011458:Prostaglandins E; D000431:Ethanol; D013392:Sucralfate; C002791:bismuth tripotassium dicitrate; D015232:Dinoprostone; D007213:Indomethacin