Attenuation of collagen arthritis and modulation of delayed-type hypersensitivity by type II collagen reactive T-cell lines.
T-cell lines were established from the lymph node cells of syngeneic Louvain (LOU) rats previously immunized with native chick type II collagen (CII) emulsified in incomplete Freund's adjuvant. The CII lines proliferated in vitro to type II collagen but not to type I collagen, ovalbumin (OV), or PPD. Control lines, developed from LOU rats immunized with OV emulsified in complete Freund's adjuvant, were OV specific because they did not respond to other antigens in vitro. CII line cells could adoptively transfer delayed-type hypersensitivity (DTH) but did not induce IgG antibody production to collagen. Moreover, the intravenous administration of 2 X 10(7) CII line cells prevented the subsequent induction of collagen arthritis following immunization and suppressed DTH to collagen without affecting antibody responses in the recipients. Spleen cells, but not sera, from these resistant rats decreased CII line reactivity in vitro. OV or irradiated CII lines had no effect on clinical or immunologic parameters in this model. These findings demonstrate protection from arthritis afforded by T-cell line transfer and suggest that the phenomenon results from down-regulation of the recipients' cellular immunity to collagen.
D000818:Animals; D001168:Arthritis / Q000276:immunology*; D001169:Arthritis, Experimental / Q000209:etiology / Q000276:immunology*; D002460:Cell Line; D003094:Collagen / Q000276:immunology* / Q000633:toxicity; D006968:Hypersensitivity, Delayed / Q000209:etiology / Q000276:immunology*; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology; D008213:Lymphocyte Activation; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D014373:Tuberculin / Q000276:immunology
D007074:Immunoglobulin G; D014373:Tuberculin; D010047:Ovalbumin; D003094:Collagen