The cellular immune response to a purified antigen from Leishmania mexicana subsp. amazonensis enhances the size of the leishmanial lesion on susceptible mice.
Immunization of BALB/c mice with gp10/20, a glycoconjugate purified from Leishmania mexicana subsp. amazonensis, induced a delayed-type hypersensitivity response to the antigen, and a significant increase was elicited in the size of the lesion induced by a subcutaneous infection with this parasite. The increase in the lesion size was observed when mice were immunized by the subcutaneous and the intraperitoneal routes. The subcutaneous immunization with gp10/20 was unable to reverse the prophylactic effect of an intravenous injection of irradiated promastigotes. An L3T4+ T-cell line specific for gp10/20 was able to transfer this lesion-enhancing effect and specific delayed-type hypersensitivity reactivity to normal syngeneic recipients. The same T-cell line was a good producer of a hematopoietic growth factor, granulocyte-macrophage colony-stimulating factor.
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000953:Antigens, Protozoan / Q000276:immunology*; D001854:Bone Marrow Cells; D006023:Glycoproteins / Q000276:immunology; D006133:Growth Substances / Q000502:physiology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular; D007114:Immunization; D007116:Immunization, Passive; D007894:Leishmania mexicana / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology* / Q000473:pathology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000953:Antigens, Protozoan; D006023:Glycoproteins; D006133:Growth Substances