Stimulation by bradykinin, angiotensin II, and carbachol of the accumulation of inositol phosphates in PC-12 pheochromocytoma cells: differential effects of lithium ions on inositol mono- and polyphosphates.
Rat PC-12 pheochromocytoma cells respond to stimulation with bradykinin, angiotensin II, and carbachol with an increased formation of labeled inositol phosphates after preincubation of the cells with [3H]inositol. Li+ potentiates greatly the agonist-induced increase in amount of inositol mono-, bis-, and trisphosphate but not the increase in amount of inositol tetrakisphosphate. Separation of the isomers of inositol trisphosphate shows that the lithium-induced increase in amount of inositol trisphosphate is due to potentiation evoked by lithium of the accumulation of inositol-1,3,4-trisphosphate.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology*; D001285:Atropine / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology*; D002414:Cations, Monovalent; D002460:Cell Line; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism*
D002414:Cations, Monovalent; D007295:Inositol Phosphates; D013403:Sugar Phosphates; D000804:Angiotensin II; D001285:Atropine; D015544:Inositol 1,4,5-Trisphosphate; D002217:Carbachol; D008094:Lithium; D001920:Bradykinin