Plasma membrane proteins from human normal and chronic myeloid leukemic granulocytes: identification and partial characterization of the concanavalin A-binding and detergent resistant proteins.
In this work granulocytes from normal human donors and patients suffering from chronic myeloid leukemia (CML) were externally labeled with 125Iodine, using the Iodogen method. 125Iodine labeled Concanavalin A binding proteins (CBP) and detergent-resistant proteins (DRP) were isolated from the cell lysates and characterized by one- and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D- and 2D-SDS-PAGE). Autoradiographs of the 2D-gels of DRP show seven proteins with Mr 118,000 (spot 1 a), Mr 112,000 (spot 1b), Mr 78,000-85,000 (spot 2), Mr 85,000 (spot 4), Mr 52,000 (spot 3, 3 a and 3 b). Of this set, spot 1 b, 2 and 4 are also present in the autoradiographs of 2D-gels of CBP and, hence, may be considered to be transmembrane components. Spot 4 is expressed more intensely in the normal granulocytes while spots 3 a and 3 b are mainly expressed on the leukemic granulocytes.
D001345:Autoradiography; D002458:Cell Fractionation; D003599:Cytoskeleton / Q000502:physiology; D003902:Detergents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006098:Granulocytes / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D007951:Leukemia, Myeloid / Q000097:blood*; D008565:Membrane Proteins / Q000032:analysis / Q000302:isolation & purification; D008970:Molecular Weight; D010449:Peptide Mapping; D011952:Receptors, Concanavalin A / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D011952:Receptors, Concanavalin A; D013501:Surface-Active Agents; C027774:concanavalin A-binding glycoproteins; D012967:Sodium Dodecyl Sulfate