[Purification of human alpha-1-protease inhibitor and production of antisera for its immunochemical determination].
Human homogeneous alpha-I-inhibitor of proteases was isolated and purified by means of chromatography on DEAE cellulose, hydroxyapatite and concanavalin Sepharose 4B. After immunization of rabbits specific monovalent antiserum towards the inhibitor was produced. The antiserum was used in diagnosis of hereditary deficiency of alpha-I-inhibitor of proteases and for estimation of its content in newborns.
D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification*; D007120:Immunochemistry; D007122:Immunoelectrophoresis; D000515:alpha 1-Antitrypsin / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D007106:Immune Sera; D000515:alpha 1-Antitrypsin
Ochistka alpha-1-ingibitora proteaz cheloveka i poluchenie antisyvorotki, prigodnoĭ dlia ego immunokhimicheskogo opredeleniia.