Differential assay of salivary and pancreatic alpha-amylase in serum and urine, with use of monoclonal antibody to human salivary amylase immobilized on bacterial cell wall.
We previously reported (Clin Chim Acta 1986;159:89) that bacterial cell wall chemically coated with a monoclonal antibody specific to human salivary (S) amylase (EC 3.2.1.1) could be successfully used to separate S and pancreatic (P) amylase in solution. We have now applied this method to serum and urine samples and found that the activities of S and P amylases so measured correlated well with those measured by the isoamylase inhibitor method. The present method is simple and reliable for routine clinical tests.
D000911:Antibodies, Monoclonal; D001419:Bacteria / Q000648:ultrastructure; D002473:Cell Wall; D006801:Humans; D007118:Immunoassay; D007527:Isoenzymes / Q000032:analysis* / Q000097:blood / Q000652:urine; D010179:Pancreas / Q000201:enzymology*; D012463:Saliva / Q000201:enzymology*; D000516:alpha-Amylases / Q000032:analysis* / Q000097:blood / Q000652:urine
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D000516:alpha-Amylases