A case of human B cell leukemia that implicates an autocrine mechanism in the abnormal growth of Leu 1 B cells.
Production of B cell growth factor (BCGF) from B-chronic lymphocytic leukemia (B-CLL) cells was demonstrated. Freshly isolated monoclonal B-CLL cells expressed surface mu, delta, B1, and Leu 1, but not Ba (an antigen expressed only on activated B cells). Upon stimulation with anti-IgM, they secreted BCGF, which could act on anti-IgM-stimulated autologous leukemic cells as well as anti-IgM-stimulated normal B cells. Cell lines established from these leukemic cells also constitutively secreted BCGF. The BCGF from B-CLL cells or established cell lines induced neither proliferation nor enhanced HLA-DR expression in resting B cells. These results show the presence of B cell-derived BCGF, which is distinct from BSF-1 and effective only on activated B cells. They also suggest that an autocrine mechanism may operate in the growth of B-CLL cells.
D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000302:isolation & purification; D006133:Growth Substances / Q000096:biosynthesis*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D015847:Interleukin-4; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis*; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D014066:Palatine Tonsil
D000954:Antigens, Surface; D006133:Growth Substances; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D015847:Interleukin-4; D004247:DNA