Immunoglobulin G-binding factors (IgG-BF) inhibit IgG secretion by, as well as proliferation of, hybridoma B cells.
Mouse immunoglobulin G-binding factors (IgG-BF) produced either by activated T cells (ATC) or by hybridoma T cells (T2D4) directly inhibit the in vitro IgG secretion by hybridoma B cells. This inhibition affects IgG1, IgG2a and IgG2b and can be detected as early as after 2 h incubation of the cells with IgG-BF eluted from non-equilibrium pH gradient electrophoresis gels. Moreover, IgG-BF also exert a strong growth-inhibitory effect on hybridoma B cells without any detectable immediate cytotoxicity. These results provide an experimental basis to analyze the molecular and biological effects induced by IgG-BF on B cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007700:Kinetics; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic
D007074:Immunoglobulin G; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors