Development and characterization of antiserum to murine granulocyte-macrophage colony-stimulating factor.
The expression in yeast of a cDNA clone encoding murine granulocyte-macrophage colony-stimulating factor (GM-CSF) has made possible the purification of large quantities of this recombinant protein. Rabbits immunized with pure recombinant GM-CSF generated antibodies that were shown to be specific for both recombinant GM-CSF and GM-CSF isolated from natural sources. Other lymphokines, including IL 1 beta, IL 2, IL 3, and recombinant human GM-CSF did not react with the antiserum. The antiserum, together with recombinant GM-CSF that had been radiolabeled with 125I to high specific activity, formed the foundation for a rapid, sensitive, and quantitative radioimmunoassay specific for murine GM-CSF. Furthermore, the antiserum was found to inhibit the biologic activities of GM-CSF as measured in both a bone marrow proliferation assay and a colony assay, and thus should prove to be a useful reagent for dissecting the complex growth factor activities involved in murine hematopoiesis.
D000818:Animals; D000918:Antibody Specificity; D001681:Biological Assay; D011232:Chemical Precipitation; D003115:Colony-Stimulating Factors / Q000276:immunology*; D006098:Granulocytes; D008264:Macrophages; D051379:Mice; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000276:immunology*
D003115:Colony-Stimulating Factors; D011994:Recombinant Proteins