Establishment of T-cell hybridoma constitutively producing macrophage-dependent T-cell replacing factor.
A T-cell hybridoma was established by the fusion of concanavalin A-stimulated splenic T cells with BW 5147. The hybridoma cells secrete a factor constitutively to support antibody formation of spleen cells depleted of T cells against TNP-Ficoll but not against horse red blood cells. The activity was indicated not to be due to interleukin 2, B-cell growth factor I, B-cell growth factor II, or interferon. The factor-mediated antibody response to TNP-Ficoll required the presence of adherent cells. The adherent cell function could be replaced by the macrophage culture supernatant containing interleukin 1. B cells responding to TNP-Ficoll in the culture with hybridoma factor were indicated to be Lyb 5+ and to bear receptors for third component of complement.
D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006133:Growth Substances / Q000096:biosynthesis; D006825:Hybridomas / Q000276:immunology*; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4; D015848:Interleukin-5; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology
D006133:Growth Substances; D007375:Interleukin-1; D007376:Interleukin-2; D015848:Interleukin-5; D008222:Lymphokines; D014301:Trinitrobenzenes; D015847:Interleukin-4