UVB/PUVA-induced suppression of human natural killer activity is reduced by superoxide dismutase and/or interleukin 2 in vitro.
We have evaluated the effects of ultraviolet irradiation or PUVA treatment [8-methoxypsoralen (8-MOP) plus long-wave ultraviolet (UVA) irradiation] on natural killer (NK) activity of human peripheral blood mononuclear cells (PBMC). In vitro exposure of PBMC to UVB (280-320 nm, 1-30 mJ/cm2) or PUVA [8-MOP, 0.1 microgram/ml; UVA (320-400 nm), 0.5-5 J/cm2] resulted in a dose-dependent suppression of NK activity, whereas UVA (0.5-5 J/cm2) or 8-MOP (0.1 microgram/ml) treatment alone did not have the inhibitory effects. This suppressive effect of UVB/PUVA irradiation was successfully reduced in the presence of superoxide dismutase (SOD) (100 or 1000 U/ml) during the irradiation. The addition of interleukin 2 (IL-2) (100 U/ml) markedly enhanced the NK activity of both irradiated and nonirradiated PBMC. Combination treatment with both SOD and IL-2 to UVB/PUVA-irradiated PBMC resulted in a more remarkable improvement of NK suppression than with either SOD or IL-2 treatment alone.
D000328:Adult; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000528:radiation effects*; D005260:Female; D006801:Humans; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000145:classification / Q000187:drug effects / Q000528:radiation effects*; D007958:Leukocyte Count / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D011701:PUVA Therapy; D010641:Phenotype; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014466:Ultraviolet Rays
D007376:Interleukin-2; D013482:Superoxide Dismutase