Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor.
Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.
D001483:Base Sequence; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000235:genetics* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006098:Granulocytes; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D008264:Macrophages; D011401:Promoter Regions, Genetic; D013329:Structure-Activity Relationship
D003115:Colony-Stimulating Factors