title: Reduced tumorigenicity of a spontaneous mouse lung carcinoma following H-2 gene transfection.
abstract: Cultured cells of the murine lung carcinoma called line 1 express very low levels of H-2 class I antigens and are resistant to lysis mediated by alloreactive T cells. In order to investigate how the expression of class I antigens affects the in vivo growth of this spontaneous tumor, H-2Dp genes were transferred into line 1 cells. Cloned transfectants that displayed H-2Dp surface antigens were identified using flow cytometry. The transfected H-2Dp antigens appeared normal by two-dimensional gel electrophoresis and could also function as excellent targets for T-cell-mediated lysis in vitro. Marked differences in tumorigenicity (defined as tumor growth in immunologically competent hosts) were observed between the Dp transfected cells and untransfected or control transfected line 1 cells in syngeneic mice only if the animals had previously received injections of irradiated Dp transfectants. Expression of Dp antigens did not appreciably affect the growth of line 1 tumors in immunologically naive syngeneic mice or necessarily cause rejection in allogeneic mice. Our in vivo results show that increased expression of class I antigens can reduce the growth of tumors like line 1 that lack all class I antigens. Our results also suggest that increasing class I antigens alone on some spontaneous tumors deficient in expression will not by itself be sufficient for tumor rejection.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000502:physiology*; D002277:Carcinoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D006084:Graft Rejection; D006183:H-2 Antigens / Q000235:genetics / Q000502:physiology*; D064532:Histocompatibility Antigen H-2D; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D014184:Transplantation, Homologous
chemical_list: D000951:Antigens, Neoplasm; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D