Application of high-performance liquid chromatography and thermospray high-performance liquid chromatography-mass spectrometry to the analysis and identification of 2',3'-dideoxyadenosine and its metabolite in biological media.
Reversed-phase high-performance liquid chromatographic (HPLC) procedures are described for determining the stability of 2',3'-dideoxyadenosine (DDA) in biological fluids at therapeutic dosages. The validated methodology uses both direct injection and solid-phase extraction techniques. Deamination of DDA to 2',3'-dideoxyinosine (DDI) in plasma by adenosine deaminase was monitored by HPLC, and the identification of DDI verified by thermospray HPLC-mass spectrometry. This methodology should prove useful in future studies concerning the stability and metabolism of dideoxynucleosides.
D000243:Adenosine Deaminase / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003839:Deoxyadenosines / Q000031:analogs & derivatives* / Q000097:blood; D016048:Dideoxyadenosine; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice
D003839:Deoxyadenosines; D016048:Dideoxyadenosine; D000243:Adenosine Deaminase