Functional heterogeneity within the human peripheral blood B cell pool engaged in IgE synthesis.
Fractionation of human peripheral blood leukocytes (PBL) B cells by differential sedimentation on percoll gradients separates B cell subpopulations which vary markedly in rates of spontaneous IgE synthesis, in their response to identical populations of autologous T cells and in their response to soluble T cell factors. This B cell fractionation technique often reveals the presence of active IgE-secreting cells which are totally suppressed within unfractionated PBL B cell preparations and greatly improves the efficiency of detection of T cell-responsive, IgE-producing B cells in PBL of atopics.
D000328:Adult; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002648:Child; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000473:pathology; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D008222:Lymphokines / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D008222:Lymphokines; D007073:Immunoglobulin E