A simple sensitive bioassay for interleukin-1 which is unresponsive to 10(3) U/ml of interleukin-2.
A subclone, NOB-1, of the mouse EL-4 line constitutively produces very little interleukin-2 but in response to interleukin-1 produces high concentrations of interleukin-2. Co-stimulation with mitogen, phorbol esters or calcium ionophores was not required. NOB-1 is not responsive to tumour necrosis factor alpha, tumour necrosis factor beta, interferon gamma and lipopolysaccharide. The NOB-1 line was used in conjunction with a CTLL line to detect less than 1 pg/ml interleukin-1. Rapid assay was performed by co-culturing the EL-4 cells with CTLL cells. By incorporating a pre-incubation step, followed by thorough washing of the EL-4 cells, responses to interleukin-1 were maintained, but interleukin-2 had no effect. The assay was used to detect interleukin-1 in serum samples and to evaluate neutralizing antisera to interleukin-1.
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000276:immunology; D007376:Interleukin-2 / Q000032:analysis* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D011994:Recombinant Proteins / Q000032:analysis*; D013379:Substrate Specificity
D007375:Interleukin-1; D007376:Interleukin-2; D011994:Recombinant Proteins