On the possibility of the unification of drug targeting systems. Studies with liposome transport to the mixtures of target antigens.
In order to make the drug targeting system more effective, simple and technological, we suggest creation of drug-bearing conjugates capable of simultaneous binding with different antigenic components of the target via specific antibodies. It is supposed that the targeted therapy should include sequential administration of the mixture of modified antibodies (or other specific vectors) against different components of affected tissue and, upon antibody accumulation in the desired region, administration of modified drugs or drug carrying systems which can recognize and bind with the target via accumulated antibodies due to the interaction between vector modifier and carrier modifier. Using as a model system monolayers consisting of the mixture of extracellular antigens and appropriated antibodies, it was shown that the treatment of the target with the mixture of biotinylated antibodies against all target components and subsequent binding with the target of biotinylated liposomes via avidin permits high liposome accumulation on the monolayer. The binding achieved is always higher than in the case of the utilization of single antibody-bearing liposomes. Besides, the system suggested is very simple and its components can be easily obtained on technological scale in standardized conditions.
D000906:Antibodies / Q000008:administration & dosage*; D000941:Antigens / Q000276:immunology*; D001360:Avidin; D001710:Biotin; D003094:Collagen / Q000276:immunology; D005340:Fibrinogen / Q000276:immunology; D005353:Fibronectins / Q000276:immunology; D006801:Humans; D000922:Immunotoxins / Q000008:administration & dosage; D007797:Laminin / Q000276:immunology; D008077:Lipoproteins, LDL / Q000276:immunology; D008081:Liposomes / Q000008:administration & dosage*; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*
D000906:Antibodies; D000941:Antigens; D005353:Fibronectins; D000922:Immunotoxins; D007797:Laminin; D008077:Lipoproteins, LDL; D008081:Liposomes; D004364:Pharmaceutical Preparations; D001360:Avidin; D001710:Biotin; D005340:Fibrinogen; D003094:Collagen