Inhibition of delta 5-3 beta hydroxysteroid dehydrogenase activity in pre-implantation mouse embryos and their implantation by sheep ovarian follicular fluid peptide.
The activity of delta 5-3 beta hydroxysteroid dehydrogenase (delta 5-3 beta HSD) in pre-implantation mouse embryos was inhibited following their prior incubation with partially purified sheep ovarian follicular fluid peptide (OFFP). OFFP, 5 microliter, injected into the uterine lumen of day 4 pregnant mice impaired the ensuing implantation of embryos. Our observations suggest that OFFP may inhibit steroid metabolizing activity in pre-implantation embryos and thereby interfere with the process of implantation.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D010064:Embryo Implantation / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D006080:Ovarian Follicle / Q000032:analysis; D010063:Ovum / Q000201:enzymology*; D010455:Peptides / Q000008:administration & dosage* / Q000302:isolation & purification; D010784:Photomicrography; D011247:Pregnancy; D012756:Sheep
D010455:Peptides; D015096:3-Hydroxysteroid Dehydrogenases