Comparative study of the effect of serum containing granulocyte-stimulating activity and colony-stimulating activity on hematopoiesis in triple-set diffusion chambers.
Serum obtained from rats with polyvinylpyrrolidone-induced sterile inflammation contains granulopoiesis-stimulating activity (GSA). In triple-set diffusion chambers (DC) with GSA in the middle chamber a stimulation of morphologically recognizable granulocytic cells (MRG) was found in mouse bone marrow cells cultivated in side chambers for 6 and 24 h. GSA also induced an increase in the number of CFU-GM when DC were inplanted into the peritoneum of normal rats. Under the same experimental conditions, colony-stimulating factor (CSA) from mouse-lung-conditioned medium did not cause such changes either in MRG or in CFU-GM compartments. The data obtained indicate the different stimulating action of GSA and CSA on granulopoiesis.
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003470:Culture Media; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D066298:In Vitro Techniques; D008297:Male; D051381:Rats
D003115:Colony-Stimulating Factors; D003470:Culture Media