Energy metabolism in adenosine deaminase-inhibited human erythrocytes.
The metabolic changes induced by the deoxycoformycin inhibition of adenosine deaminase were studied in human erythrocytes incubated with nucleosides. 1 Adenosine nucleotide levels and glycolytic rate were increased by adenosine. 2 With deoxyadenosine, the cellular ATP level was reduced when dATP increased and the glycolytic rate was similarly enhanced. 3 The hypoxanthine production was equivalent in both cases. Our data demonstrate that human red cells are able to catabolize adenine deoxynucleotides into hypoxanthine, and the control of energy metabolism is not impaired by adenosine deaminase inhibition when PO identical to 4 and NAD+ are not limiting.
D000241:Adenosine / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000097:blood; D058892:Adenosine Deaminase Inhibitors; D000244:Adenosine Diphosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D003070:Coformycin / Q000031:analogs & derivatives / Q000494:pharmacology*; D003838:Deoxyadenine Nucleotides / Q000097:blood; D003839:Deoxyadenosines / Q000494:pharmacology; D004734:Energy Metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000097:blood; D009700:Nucleoside Deaminases / Q000037:antagonists & inhibitors*; D015649:Pentostatin; D012263:Ribonucleosides / Q000494:pharmacology*
D058892:Adenosine Deaminase Inhibitors; D003838:Deoxyadenine Nucleotides; D003839:Deoxyadenosines; D007042:Hypoxanthines; D012263:Ribonucleosides; D003070:Coformycin; D019271:Hypoxanthine; D015649:Pentostatin; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000241:Adenosine; C026600:2'-deoxyadenosine triphosphate