Identification and quantitation of the expression of T cell surface markers during the development of chronic relapsing experimental allergic encephalomyelitis (CREAE) in the guinea pig.
As there were discrepancies in previous data on the T cell nature of cells infiltrating the meninges at all stages of chronic relapsing experimental allergic encephalomyelitis (CREAE), experiments have been performed using a further monoclonal antibody (Mab) recognizing total T cell populations and the classic E rosetting technique. Cytospins were prepared of the meningeal inflammatory cells obtained by washing the brains of these animals, and stained by indirect immunoperoxidase. It was found that the T cell, as defined by both E rosetting and staining with the Mab CT5, is the major cell type found in the meninges during the development of CREAE. However, the staining with the Mab CT7, which recognizes a functionally relevant antigen, showed that there is a discrepancy between the numbers of lymphocytes stained compared to the results with CT5 and E rosettes. Furthermore, the antigen recognized by CT7 appeared to be modulated during the disease. The possible functional relevance and its relation to clinical remission and relapse are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002908:Chronic Disease; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D008297:Male; D008578:Meninges / Q000276:immunology*; D011817:Rabbits; D012008:Recurrence; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface