title: Re-evaluation of LG V of the rat and assignment of 12 carboxylesterases to two gene clusters.
abstract: Examining the strain distribution pattern of the recombinant inbred strain series LXB and DXE and of backcross progeny of (LEW X LE)F1 X LEW, (LEW X BN)F1 X LEW, and (LEW X BN)F1 X BN for esterase markers, including three carboxylesterase allozymes (ES-15, ES-16, ES-18), hitherto not studied genetically, revealed the existence of two esterase gene clusters within LG V: cluster 1, containing Es-2, Es-8, Es-10, Es-3, Es-7, Es-9, and separated by 8.8 +/- 1.3 cM from cluster 2, containing Es-1, Es-14 (formerly Es-Si), Es-15, Es-16, and Es-18. Analyses of 93 inbred strains of rats showed only 12 and 6 haplotypes for cluster 1 and cluster 2, respectively, indicating a strong linkage disequilibrium. These data and serotyping results of one backcross population for the RT2 blood group system lead to a re-evaluation of linkage group V. Including literature data the following gene order is suggested: RT2 - (7.1 +/- 1.8) Es-2, Es-4, Es-8, Es-10 (2.7 +/- 0.7) Es-3, Es-7, Es-9 (8.8 +/- 1.3) Es-1, Es-14, Es-15, Es-16, Es-18.
mesh_terms: D000483:Alleles; D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D006239:Haplotypes; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D009928:Organ Specificity; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D013045:Species Specificity
chemical_list: D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase