title: Induction of concanavalin A dose-dependent T-cell growth factor production by insertion of T-cell membrane components into the AKR thymic lymphoma BW 5147.
abstract: Sendai virus vesicles were used as vehicles for the insertion of various cell membranes into different cell lines. The transplantation efficiency was measured by using FITC-labelled concanavalin A (Con A) or monoclonal antibodies against the T-cell marker Lyt 2 and the major histocompatibility complex product H-2Db in a fluorescence-activated cell sorter. Results indicate that it is possible to transplant mitogen responsiveness to certain cell types. Con A stimulation of T-cell membrane transplanted BW 5147 showed that it is possible to induce a mitogen dose-dependent T-cell growth factor production. Consequently this method appears to be an attractive model for further study of the properties of the membrane structures involved in mitogen triggering of cells.
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism* / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D007376:Interleukin-2 / Q000096:biosynthesis*; D007939:Leukemia L1210 / Q000276:immunology; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000502:physiology*; D011952:Receptors, Concanavalin A / Q000032:analysis / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism*; D013953:Thymus Neoplasms / Q000378:metabolism*
chemical_list: D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D011952:Receptors, Concanavalin A; D003208:Concanavalin A