A human T lymphoblastic cell line lacks lamins A and C.
Lamins A, B and C, the three major proteins of nuclear envelope, constitute a class of intermediate filament polypeptides. We have compared the amount of these polypeptides in two human cell lines, epithelial HeLa cells and T lymphoblasts KE 37. It was found that the three lamins were present in roughly equimolar stoichiometry in HeLa cells, while lamin B was the unique lamin component in T lymphoblasts. Moreover, 3-kb mRNA of lamin A and 2.1-kb mRNA of lamin C were detected with a human cDNA probe in HeLa cells but not in T lymphoblasts. These results suggest that (i) lamin B can build up the lamina structure in actively dividing somatic cells by itself, and (ii) lamin expression in lymphoid cells may be subject to important quantitative variations. Comparison of the lamin composition of human cloned T lymphocytes and Epstein-Barr virus-transformed human B lymphocytes confirmed this statement. The lamin B level was nearly equivalent in both cells but the content of lamins A and C varied to a large extent, being low in T cells and high in B cells.
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D007525:Isoelectric Focusing; D034904:Lamin Type A; D034921:Lamin Type B; D034882:Lamins; D009363:Neoplasm Proteins / Q000032:analysis; D009685:Nuclear Envelope / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D013601:T-Lymphocytes
D034904:Lamin Type A; D034921:Lamin Type B; D034882:Lamins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D012334:RNA, Neoplasm; C440308:lamin C