Human recombinant interleukin 1-mediated suppression of glycosaminoglycan synthesis in cultured rat costal chondrocytes.
Effects of human recombinant interleukin 1 (IL-1) on the synthesis of glycosaminoglycan were examined with cultured rat costal chondrocytes. Incorporation of [35S]sulfate into glycosaminoglycan was strikingly diminished by the addition of IL-1 in a dose- and time- dependent manner. When the cells were cultured with 340 micrograms/ml of IL-1 for 72 hr, the synthesis of glycosaminoglycan was inhibited to 10% of the control. On the other hand, IL-1 had no effect on the morphology and proliferation of the chondrocytes. The suppression of glycosaminoglycan synthesis remained unchanged after the addition of indomethacin, indicating that the effect of IL-1 is independent of the enhanced synthesis of prostaglandins.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015232:Dinoprostone; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D007375:Interleukin-1; D008297:Male; D011458:Prostaglandins E / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism*
D006025:Glycosaminoglycans; D007375:Interleukin-1; D011458:Prostaglandins E; D011994:Recombinant Proteins; D013431:Sulfates; D015232:Dinoprostone; D002118:Calcium