Characterization of Lyt-2-, L3T4- class I-specific cytolytic clones in C3H-gld/gld mice. Implications for functions of accessory molecules and programmed development.
We report the first demonstration of Thy-1+, Lyt-2-, L3T4- MHC-specific CTL clones derived from the Lyt-2-, L3T4- subset of lymph node cells of C3H-gld/gld mice. These clones express alpha/beta heterodimeric TCRs on the cell surface and specifically recognize class I molecules on target cells. Lyt-2 and L3T4 molecules are therefore not essential for the induction, recognition, and killing of antigen-specific CTL. In addition, these studies suggest that antigen specificity development for class I structures may occur before Lyt-2 gene activation in the differentiation of T cells.
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis*; D002999:Clone Cells / Q000032:analysis*; D007518:Isoantibodies / Q000032:analysis; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology*
D000950:Antigens, Ly; D007518:Isoantibodies; C029805:Lyt antibodies; D011948:Receptors, Antigen, T-Cell