On-line recording of vascular resistance in isolated canine renal artery by use of a differential pressure transducer.
For the assessment of the validity of the perfusion system of vascular segments, both isolated canine renal artery in cylindrical form and artificial tubing were perfused intra- and extraluminally, and vascular resistance was continuously recorded by use of a differential pressure transducer. The model experiments using artificial tubing showed that the vascular resistance, inversely related to outflow and differential pressure, enormously increased when the lumen was occluded more than 85% of the control. Various agonistic stimuli such as norepinephrine, phenylephrine, methoxamine, prostaglandin F2 alpha and 5-hydroxytryptamine, when administered intraluminally, increased the vascular resistance, while verapamil and papaverine decreased it. Transmural electrical stimulation also increased the resistance which was susceptible to tetrodotoxin. The removal of endothelium by infusion of saponin augmented the contractile responses to agonistic stimuli such as norepinephrine and 5-hydroxytryptamine. The results indicate that the present perfusion method seems to be a good alternative for studying contractile activity and endothelium function of isolated vascular tissues.
D000818:Animals; D015237:Dinoprost; D004285:Dogs; D004558:Electric Stimulation; D004727:Endothelium / Q000502:physiology; D005260:Female; D008297:Male; D008729:Methoxamine / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010208:Papaverine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D012077:Renal Artery / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology; D014160:Transducers, Pressure; D014655:Vascular Resistance / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology
D011460:Prostaglandins F; D010656:Phenylephrine; D012701:Serotonin; D015237:Dinoprost; D014700:Verapamil; D010208:Papaverine; D008729:Methoxamine; D009638:Norepinephrine