Radioimmunoassay of urinary 21-deoxytetrahydroaldosterone in primary aldosteronism and 21-hydroxylase deficiency.
A radioimmunoassay of 21-deoxytetrahydroaldosterone was developed. Normal daily excretion of the unconjugated metabolite was 1.2 +/- 1.3 micrograms and of the glucuronized metabolite, 11.9 +/- 7 micrograms. The tetrahydroaldosterone/21-deoxytetrahydroaldosterone ratio varied more in patients with primary aldosteronism than in control subjects. Thus, measurements of the urinary excretion of the tetrahydroaldosterone or 21-deoxytetrahydroaldosterone alone did not provide an accurate expression for aldosterone production. Their sum correlated well with the clinical condition, i.e. clear-cut elevation in patients with primary aldosteronism. The diminished tetrahydroaldosterone/21-deoxytetrahydroaldosterone ratio found in patients with 21-hydroxylase deficiency may be attributed to increased bacterial conversion of tetrahydroaldosterone to 21-deoxytetrahydroaldosterone but could also stem from a deficiency implicating zona glomerulosa (aldosterone biosynthesis) regardless of the stage and clinical presentation of the disease.
D000312:Adrenal Hyperplasia, Congenital / Q000652:urine*; D000450:Aldosterone / Q000031:analogs & derivatives* / Q000652:urine; D002648:Child; D005260:Female; D006801:Humans; D006929:Hyperaldosteronism / Q000652:urine*; D008297:Male; D011863:Radioimmunoassay; D012016:Reference Values; D013250:Steroid Hydroxylases / Q000172:deficiency*
D000450:Aldosterone; C047002:21-deoxytetrahydroaldosterone; D013250:Steroid Hydroxylases