Antigenic relationships among penicillin-binding proteins 1 from members of the families Pasteurellaceae and Enterobacteriaceae.
Penicillin-binding proteins (PBPs) from Haemophilus influenzae RD purified by a combination of affinity chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and electroelution were used to immunize rabbits to obtain specific antisera. Antisera directed against PBP 1 (1b) of H. influenzae cross-reacted with representative organisms of the family Pasteurellaceae and with many members of the family Enterobacteriaceae but not with other gram-negative organisms. Immunization with purified PBP 3 of H. influenzae produced antisera that reacted with PBP 1 (1b) of H. influenzae and showed the same cross-reactive pattern with other species as the anti-PBP 1 antiserum. A 24,000-molecular-weight polypeptide of H. influenzae, not radiolabeled by [35S]penicillin, reacted with antisera against purified PBPs 1 (1a, 1b), 2, and 3. The results suggest that antigenic epitopes are shared among similar PBPs from related species and even among different PBPs within the same species.
D000217:Acyltransferases / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000276:immunology*; D006090:Gram-Negative Bacteria / Q000276:immunology; D006193:Haemophilus influenzae / Q000276:immunology; D006602:Hexosyltransferases / Q000276:immunology* / Q000302:isolation & purification; D007106:Immune Sera / Q000276:immunology; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000276:immunology* / Q000302:isolation & purification; D002267:Muramoylpentapeptide Carboxypeptidase; D010325:Pasteurella / Q000276:immunology*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D013045:Species Specificity
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D007106:Immune Sera; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D000217:Acyltransferases; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase