The use of solid-state image sensor technology to detect and characterize live mammalian cells growing in tissue culture.
Arguments are presented that approximately 30 000 cells have to be measured in a single experiment to measure radiobiological parameters in a low-dose survival assay. For this purpose, a fully automated device capable of detecting and recognizing individual live unstained mammalian cells at a rate of 1 cm2/min is required. Specifications of such a system are derived and evidence is presented which suggests that this can best be carried out using a solid-state image sensor in the form of a linear array of photodetectors. The outline of the design and other potential uses of such a device are discussed.
D000818:Animals; D002452:Cell Count / Q000295:instrumentation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D046508:Culture Techniques / Q000295:instrumentation*; D004307:Dose-Response Relationship, Radiation; D004867:Equipment Design; D005260:Female; D010053:Ovary; D012666:Semiconductors