title: Quantitative analysis of the cellular constituents of the cerebrospinal fluid in chronic relapsing experimental allergic encephalomyelitis.
abstract: Cerebrospinal fluid (CSF) was taken from guinea pigs in various stages of chronic relapsing experimental allergic encephalomyelitis (CR-EAE). The leucocytes in CSF samples were counted and subjected to immunocytochemical analysis using monoclonal antibodies selectively recognising guinea pig T cells, macrophages or Ia antigens. The results showed that total leucocyte numbers and the proportion of macrophages in CSF were elevated in the acute phase of CR-EAE but samples of CSF from animals in early relapse did not show a significant elevation in leucocyte count or macrophage content. In addition the level of T cell activation was higher in CSF than in peripheral blood during disease and was highest in the acute and immediately post-acute phases of the CR-EAE.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D002555:Cerebrospinal Fluid / Q000166:cytology*; D002908:Chronic Disease; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000134:cerebrospinal fluid*; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007958:Leukocyte Count; D007962:Leukocytes / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology; D008264:Macrophages / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D000949:Histocompatibility Antigens Class II