Eosinophil production in human bone-marrow cultures induced by 80-85 kDa serum component(s) of patients with eosinophilia.
Eosinophil production from normal human bone-marrow cells was induced by plasma or serum of patients with eosinophilia. After 10-12 d of liquid suspension culture, 14-42% of bone-marrow cells were identified as maturing eosinophil granulocytes. Upon molecular sieve chromatography, the activity eluted in a single peak corresponding to a substance of 80-85 kDa molecular weight. The activity did not stimulate colony formation in semi-solid agar media, but was detected in liquid suspension cultures only. Furthermore, addition of exogenous colony-stimulating factor (CSF) had a differential effect depending on the culture system used. In semi-solid media, predominance of eosinophil colonies was only observed when CSF was added at a low concentration. In liquid suspension cultures, however, 30-40% of cultured cells were eosinophils independent of the CSF concentration used. These findings extend recent studies in an animal model and support the hypothesis that a differentiation factor is involved in the regulation of eosinophil production in man.
D001853:Bone Marrow / Q000648:ultrastructure*; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003470:Culture Media; D004802:Eosinophilia / Q000097:blood*; D004804:Eosinophils / Q000648:ultrastructure*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D015847:Interleukin-4; D007958:Leukocyte Count; D008222:Lymphokines / Q000494:pharmacology*; D008854:Microscopy, Electron; D008970:Molecular Weight; D013997:Time Factors
D003115:Colony-Stimulating Factors; D003470:Culture Media; D006133:Growth Substances; D008222:Lymphokines; D015847:Interleukin-4