Optical recording of action potential propagation in demyelinated frog nerve.
Conduction in focally demyelinated frog nerves has been measured optically using potential-sensitive dyes. Absorption changes were recorded with an array of photodiodes positioned in the image plane of a microscope. Both the amplitude and conduction velocity of the optical signals decreased in the demyelinated region. Conduction was improved after exposure to the potassium channel blocking agent 4-aminopyridine.
D000200:Action Potentials; D000818:Animals; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008853:Microscopy / Q000379:methods; D009186:Myelin Sheath / Q000502:physiology*; D009431:Neural Conduction; D011894:Rana pipiens; D012584:Sciatic Nerve / Q000502:physiology*; D013053:Spectrophotometry / Q000379:methods