Molecular complexity of Qa-2 antigens demonstrated by two-dimensional gel electrophoresis.
Biosynthetically labelled Qa-2 antigens were isolated from mouse spleen cells by immunoprecipitation with anti-Qa-2 antisera. When newly synthesized Qa-2 molecules from several different inbred strains were analysed by two-dimensional (2D) gel electrophoresis; four different phenotypes were observed that differed in the number of polypeptides present. The ability to distinguish Qa-2+ phenotypes was used to map the recombination points in two congenic strains, B6.Tlaa and A.Tlab. No alternative Qa-2-like polypeptides were detected in B6.K1 (Qa-2-) cells using a polyspecific rabbit antiserum against mouse class I antigens, but a new molecule was detected in BALB/cBy (Qa-2-) cells. Pulse-chase and surface-labelling experiments showed that some, but not all, of the newly synthesized Qa-2 precursor forms are processed to mature cell surface molecules.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D006183:H-2 Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology*; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification; D011498:Protein Precursors / Q000235:genetics / Q000302:isolation & purification; D011995:Recombination, Genetic
D000954:Antigens, Surface; D005819:Genetic Markers; D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I; D010455:Peptides; D011498:Protein Precursors; C032907:Q surface antigens