Streptavidin assay by means of biotinylated actinophages.
Using D-biotinyl-N-hydroxysuccinimide ester, biotin was covalently attached to actinophage SLE 111. The chemically modified actinophage preparation (SLE 111-BIO) was prevented from forming plaques by biotin-binding proteins, such as streptavidin and avidin. The avidin concentration at 50% inactivation of biotinylated actinophages was 0.5 micrograms/ml. The streptavidin assay was useful as pre-selection method during the screening and improvement work with streptavidin-producing microorganisms.
D001360:Avidin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D001435:Bacteriophages / Q000187:drug effects / Q000254:growth & development*; D001681:Biological Assay; D001710:Biotin / Q000378:metabolism*; D019809:Streptavidin; D013302:Streptomyces
D001426:Bacterial Proteins; D001360:Avidin; D001710:Biotin; D019809:Streptavidin