[CRF-like immunoreactivities in mammalian brains examined with a radioimmunoassay for CRF].
A sensitive and useful radioimmunoassay for CRF was established using synthetic ovine CRF. CRF-like immunoreactivities of hypothalami in the bovine, human, rat, guinea pig, and mouse could be detected with this radioimmunoassay. A variety of CRF-like immunoreactivities of hypothalami in the mammalian brains led us to discuss a putative species difference of amino-acid structure in CRF. In the bovine, CRF-like immunoreactivities of the frontal cortex, caudate nucleus, putamen, and hippocampus could be detected in addition to CRF-like immunoreactivity of hypothalamus. These observations suggested a wide distribution of CRF in the central nervous system, and gave room for discussion about a physiological role of extrahypothalamic CRF. Gel chromatographic studies revealed a high-molecular-weight form of CRF-like immunoreactivity in the bovine frontal cortical profile that was not found in the hypothalamic profile. The results show a multiplicity of CRF-like immunoreactivity in the bovine brain.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002421:Caudate Nucleus / Q000032:analysis; D003346:Corticotropin-Releasing Hormone / Q000032:analysis*; D005625:Frontal Lobe / Q000032:analysis; D006168:Guinea Pigs; D006624:Hippocampus / Q000032:analysis; D006801:Humans; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011699:Putamen / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D003346:Corticotropin-Releasing Hormone