Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.
A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.
D000469:Alkaline Phosphatase; D008401:Gas Chromatography-Mass Spectrometry; D006193:Haemophilus influenzae / Q000032:analysis*; D006868:Hydrolysis; D008070:Lipopolysaccharides; D010766:Phosphorylation; D013400:Sugar Acids / Q000302:isolation & purification*
D008070:Lipopolysaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D000469:Alkaline Phosphatase