Determination of tenoxicam in human plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic method for the determination of tenoxicam in plasma has been developed. Tenoxicam was extracted from buffered plasma (pH 3 or 4, respectively) with dichloromethane and the evaporated extracts were analysed on a C18 reversed-phase column using a methanol-phosphate buffer mobile phase and with UV detection at 371 nm. The detection limit was 20 ng/ml using a 0.5-ml sample. The method is selective with respect to the 5'-hydroxy metabolite, which is present in plasma after multiple administration of tenoxicam; this metabolite may also be determined using this procedure.
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008027:Light; D010894:Piroxicam / Q000031:analogs & derivatives*; D013843:Thiazines / Q000097:blood*; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D013843:Thiazines; D010894:Piroxicam; C032801:tenoxicam