Purification of human interleukin 2 produced from normal human peripheral blood mononuclear cells.
We have developed a simple protocol for the routine purification of essentially homogeneous human interleukin 2. The procedures applied include ammonium sulfate precipitation, ACA-54 gel filtration, ultrafiltration and chromatofocusing. The product has a molecular weight of 14 000, as determined by electrophoretic mobility, and is free of interleukin 1, interferon, granulocyte and monocyte stimulating factors, B cell growth factor and phytohemagglutinin. The method is efficient, rapid and reproduceable and provides a helpful method for preparation of IL-2 for biochemical and biological studies at moderate cost and without the use of complex equipment.
D000645:Ammonium Sulfate / Q000494:pharmacology; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods; D003470:Culture Media / Q000032:analysis; D006801:Humans; D007376:Interleukin-2 / Q000302:isolation & purification*; D007526:Isoelectric Point; D008214:Lymphocytes / Q000032:analysis*; D008970:Molecular Weight
D003470:Culture Media; D007376:Interleukin-2; D000645:Ammonium Sulfate