Laser-induced fusion of mammalian cells and plant protoplasts.
An ultraviolet-laser microbeam was shown to be suitable for inducing fusion of individually selected plant protoplasts or of B-lymphocytes with myeloma cells. The fusion took place in normal culture medium and the fusogenic condition perturbed the cells only for a fraction of a millisecond. Without manipulating the cell culture except for exposing the cells to laser light, fusion products between preselected individual pairs may be produced.
D000818:Animals; D001402:B-Lymphocytes / Q000528:radiation effects*; D001937:Brassica / Q000166:cytology; D002459:Cell Fusion; D002478:Cells, Cultured; D006822:Hybrid Cells / Q000528:radiation effects; D007834:Lasers; D011523:Protoplasts / Q000528:radiation effects*; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014466:Ultraviolet Rays