Interleukin-1 decreases the level of thymocytes forming rosettes with autologous erythrocytes: a new method of determination of interleukin-1 activity.
It was demonstrated that human, recombinant Il-1 significantly inhibited formation of autologous rosettes by thymocytes. In addition, Il-1 increased the percentage of thymocytes resistant to hydrocortisone. Il-2 was not active in both tests. A new method of determination of Il-1 activity, based on the inhibition of the autologous rosette formation by Il-1 is described. The activity of Il-1 in the supernatant from macrophage cultures, stimulated with carbonyl iron powder, was calculated. The lack of Il-2 interference and no necessity to handle radioactive materials, are the advantages of the new method.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004351:Drug Resistance; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000032:analysis* / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D007375:Interleukin-1; D006854:Hydrocortisone