title: Lithium augments GM-CSA generation in canine cyclic hematopoiesis.
abstract: Cyclic hematopoiesis in gray collie dogs can be cured by lithium treatment. We examined the mechanism of lithium's effect by developing an assay for the canine equivalent of GM-CSF (called GM-CSA). Phytohemagglutinin (PHA)-stimulated canine blood mononuclear cells produce GM-CSA in a dose-dependent manner; this GM-CSA stimulates more neutrophil-containing colonies than does endotoxin-treated dog serum. Production of GM-CSA by PHA-stimulated normal dog cells was not altered by lithium. However, cells from gray collies during their neutrophilic period increased their GM-CSA when lithium (2 mEq/L) was added to low doses of PHA, whereas neutropenic gray collie cells did not. These data suggest that lithium could modulate cyclic hematopoiesis by increasing intramedullary GM-CSA at the time when marrow neutrophilic progenitor cells are at their nadir.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003524:Cyclosporins / Q000494:pharmacology; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D005260:Female; D006402:Hematologic Diseases / Q000188:drug therapy / Q000662:veterinary*; D006410:Hematopoiesis / Q000187:drug effects*; D007377:Interleukin-3 / Q000096:biosynthesis*; D008094:Lithium / Q000494:pharmacology* / Q000627:therapeutic use; D008264:Macrophages / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000502:physiology; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D003524:Cyclosporins; D007377:Interleukin-3; D010835:Phytohemagglutinins; D008094:Lithium