Modulation of in vitro myelopoiesis by alloreactive T cell clones.
Regulatory effects of mixed lymphocyte culture (MLC)-derived CD4+ human T cell clones on granulocyte-macrophage colony (CFU-GM) formation by normal bone marrow (BM) were studied in an initial attempt to establish an in vitro model for the negative feedback control of myelopoiesis by alloactivated T cells. This is likely to be of clinical significance in the aberrant control of haematopoiesis during some cases of graft-versus-host disease (GVHD) after allogeneic BM transplantation. Whilst 5 such alloproliferative clones generally failed to suppress CFU-GM, the majority of clones with natural killer (NK)-like activity, or those with suppressive activity in MLC, regularly and strongly suppressed in this system, reinforcing the view that certain T cells may have potent negative regulatory effects on haematopoiesis.
D001854:Bone Marrow Cells; D002999:Clone Cells / Q000378:metabolism; D003114:Colony-Forming Units Assay; D006086:Graft vs Host Disease / Q000276:immunology; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006801:Humans; D007694:Killer Cells, Natural / Q000032:analysis / Q000276:immunology; D008264:Macrophages / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014184:Transplantation, Homologous