Simple preparative two-step purification of interleukin-2 from culture medium of lectin stimulated normal human lymphocytes.
A procedure has been developed for the rapid purification of human IL-2 using reversed-phase liquid chromatography on LiChroprep RP-8 and Separon SGX C-18. The resulting IL-2 has a specific activity of 1 X 10(7) U/mg protein and reveals in SDS-PAGE 2 molecular weight forms (approximately 14 kDa and approximately 16 kDa) under reducing and non-reducing conditions. Both forms are biologically active. In 2-dimensional electrophoresis purified IL-2 shows a complex pattern of protein spots located in the IL-2 region which may be due to differences in glycosylation.
D002853:Chromatography, Liquid; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005615:Freezing; D006801:Humans; D007376:Interleukin-2 / Q000302:isolation & purification*; D008214:Lymphocytes / Q000032:analysis* / Q000187:drug effects; D011309:Preservation, Biological; D013154:Spleen / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D007376:Interleukin-2; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate