Effect of aluminium on mineralization of rat third molar in vitro.
Crl: COBS rat third-molar explants were cultured for 12 days in either 0.6 X 10(-2), 4 X 10(-2) or 6 X 10(-2) mM aluminium, or for 12 days with exposure to 13 X 10(-2) mM Al at different 6-day intervals. Total protein, alkaline phosphatase, calcium and phosphorous were measured to evaluate cell viability and the degree of mineralization. Al in concentrations above 4 X 10(-2) mM significantly reduced the Ca and P content of explants cultured for 12 days. Explants exposed to 13 X 10(-2) mM Al for the first 6 days had less Ca and P than those exposed for the last 6 days of culture. Haematoxylin and eosin-stained sections of explants showed no gross abnormalities.
D000469:Alkaline Phosphatase / Q000032:analysis; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000032:analysis; D008964:Molar, Third; D009924:Organ Culture Techniques; D010758:Phosphorus / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014070:Tooth / Q000032:analysis / Q000187:drug effects*; D014074:Tooth Calcification / Q000187:drug effects*
D010758:Phosphorus; D000535:Aluminum; D000469:Alkaline Phosphatase; D002118:Calcium