Identification of incomplete coding sequences for steroid sulphatase on the human Y chromosome: evidence for an ancestral pseudoautosomal gene?
A cDNA clone (p422) containing about 200bp of coding sequences for steroid sulphatase (STS) has been isolated from a lambda gt11 expression library by antibody screening and has been assigned by mapping with a somatic cell hybrid panel and by in situ hybridization to Xp22.3; a localization coincident with the previously identified locus for STS expression. Although no significant hybridization of this clone to the Y chromosome was observed, p422 has been used to isolate a longer cDNA clone and genomic sequences which do recognize Y-specific restriction fragments. An abbreviated STS gene has been localized to Yq11.2. The coding sequences for the human enzyme shows little homology to sequences in mice.
D000818:Animals; D001192:Arylsulfatases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000235:genetics*; D014960:X Chromosome; D014998:Y Chromosome
D004247:DNA; D013429:Sulfatases; D001192:Arylsulfatases; D043266:Steryl-Sulfatase