Forssman glycolipid, an antigenic marker for a major subpopulation of macrophages from murine spleen and peripheral lymph nodes.
Three hybridoma clones were isolated after hybridization of a mouse myeloma line with splenocytes from rats immunized with Forssman glycosphingolipid (Fo). Two of these clones produced Fo-specific monoclonal antibodies (MAB) of the IgM class, one MAB of the IgG2c class. In complement-dependent depletion experiments and immunofluorescence studies on the nature of Fo-positive leukocytes in CBA/J mice the following results were obtained: whereas blood monocytes, polymorphonuclear leukocytes, and lymphocytes were Fo negative, 5 to 10% of suspended spleen cells were positive. The majority of these were macrophage-like, glass- and nylon-adherent, nonspecific esterase-positive phagocytizing cells carrying Ia and globoside markers. These cells participated as accessory cells in the mixed lymphocyte culture reaction. In cell suspensions from axillary and inguinal lymph nodes, 2% were Fo positive. They were enriched up to 70% in the glass-adherent, esterase-positive population from this source. In contrast, no Fo-positive cells were detected in mesenteric lymph nodes, and less than 0.1% of the resident peritoneal macrophages bore this marker. The percentage of Fo-positive cells increased to 1% in thioglycollate-elicited peritoneal cells. Immunostaining of cryosections of lung and liver tissue showed alveolar macrophages and Kupffer cells, respectively, to be Fo negative.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015478:Antigens, Heterophile / Q000032:analysis*; D002469:Cell Separation; D005260:Female; D005577:Forssman Antigen / Q000032:analysis* / Q000276:immunology; D005915:Globosides / Q000032:analysis* / Q000276:immunology; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology*; D008264:Macrophages / Q000145:classification* / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D010641:Phenotype; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology*
D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D005915:Globosides; D006028:Glycosphingolipids; C010163:Forssman glycolipid; D005577:Forssman Antigen