Antiproliferative response of human leukemic cells. Modulation of cytosolic protein kinase C activity by phytohemagglutinin.
Phytohemagglutinin and its isolectins PHA-E4 an PHA-L4 act antiproliferatively on an actively dividing leukemia T-cell line. Both PHA and the isolectins caused an increase in soluble protein kinase C (PK-C) activity without a corresponding decrease in particulate activity. The increase was at a maximum after 10 min and the soluble kinase activity remained high for at least 3 h. There was no direct correlation between the observed antiproliferative potency of the 2 isolectins and their ability to initially affect the distribution of PK-C activity.
D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011493:Protein Kinase C / Q000032:analysis*; D014407:Tumor Cells, Cultured
D037102:Lectins; D010835:Phytohemagglutinins; D011493:Protein Kinase C