The interaction of prostaglandins with high-density lipoproteins: a non-equilibrium model of ligand-receptor interaction.
Using high-density lipoproteins (HDL) labeled with a fluorescent phospholipid probe (an anthrylvinyl-labeled analogue of sphingomyelin) it was found that low amounts (10(-12) M) of the prostaglandins E1 and F2 alpha induced different structural changes of the HDL surface, whereas prostaglandin E2 had no effect. The effects of prostaglandin E1 on HDL were largely paralleled by those of this prostaglandin on synthetic recombinants prepared from apolipoprotein A1, phospholipids and cholesterol. The prostaglandin E1-HDL interaction resembled that of a ligand with a receptor site because it was specific, reversible, concentration- and temperature-dependent and saturable. However, the maximal HDL retaining capacity for prostaglandin E1 as determined by equilibrium dialysis was very low, and a single prostaglandin E1 molecule was able to induce structural changes in a large number of discrete lipoprotein particles. To explain this remarkable fact, a non-equilibrium model of ligand-receptor interaction is proposed. According to this model in open systems characterized by a short life-time of the ligand-receptor complex, high diffusion rates of the ligand and long relaxation times which exceed the interval between two successive ligand-receptor occupations, the ligand-induced changes will accumulate, resulting in amplification of the primary biological signal. It is emphasized that the low mobility of lipids constituting the environment of the receptor protein plays a critical role in this type of signal amplification.
D000527:Alprostadil / Q000097:blood*; D001054:Apolipoproteins A / Q000097:blood; D015237:Dinoprost; D004735:Energy Transfer; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D011460:Prostaglandins F / Q000097:blood; D013050:Spectrometry, Fluorescence; D013109:Sphingomyelins / Q000097:blood
D001054:Apolipoproteins A; D005456:Fluorescent Dyes; D008075:Lipoproteins, HDL; D011460:Prostaglandins F; D013109:Sphingomyelins; C053698:N-12-(anthryl)-11-dodecenoylsphingosine-1-phosphorylcholine; D015237:Dinoprost; D000527:Alprostadil