Effect of prostaglandins on Cl and K transport in rat submandibular salivary acini.
Dispersed acini were used to investigate the effects of prostaglandins (PG) on transmembrane Cl and K transport with the aid of radioactive tracers. Neither PGE1, PG2, PGF2 alpha, arachidonic acid or phosphatidic acid (all in 1 microM final concentrations) modified the time-dependent uptake of 36Cl. Steady-state isotope content reached 6-7 nmol/mg protein with or without these substances. PG did not alter the inhibitory effect of 1 mM furosemide on 36Cl uptake, and failed to induce a net efflux of 36Cl from tracer-preloaded cells or to modify the efflux of tracer induced by 1 microM acetylcholine. PG had no significant effect on K uptake, as measured with 86Rb, and did not modify the effect of 1 mM ouabain, which inhibited K uptake or accumulation by 60 per cent. PG did not induce K (86Rb) efflux from acini preloaded with tracer, and did not prevent or enhance the K efflux induced by acetylcholine. Thus PG do not influence the major ion-transport systems that may be involved in saliva secretion by acinar cells. Any inhibitory effects of PGE1 on salivary-fluid secretion in vivo are therefore likely to be the result of extra-acinar PG actions, and not of a direct effect on ion-transport mechanisms.
D000109:Acetylcholine / Q000494:pharmacology; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002712:Chlorides / Q000493:pharmacokinetics*; D015237:Dinoprost; D015232:Dinoprostone; D011188:Potassium / Q000493:pharmacokinetics*; D011453:Prostaglandins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000187:drug effects* / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism
D002712:Chlorides; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D015237:Dinoprost; D000527:Alprostadil; D015232:Dinoprostone; D000109:Acetylcholine; D011188:Potassium