Three kinds of tumour-unique surface molecules on a human T-cell chronic lymphocytic leukaemia (T-CLL) detected by monoclonal antibodies.
Mouse monoclonal antibodies recognizing cell surface molecules on tumour cells from a patient with a T-cell chronic lymphatic leukaemia (T-CLL) have been produced. Three different types of idiotype-like cell surface structures were identified. One molecule had a relative molecular weight (Mr) of 90,000 under non-reduced conditions and of 42,000 upon reduction, which corresponds well to the T-cell receptor for antigen (Ti). The two other molecules, which also behaved like unique tumour markers, have not previously been described, to our knowledge. One molecule was a monomer with an Mr of 74,000-80,000 when non-reduced and 80,000 upon reduction. The other idiotypic molecule was a dimer with a non-reduced Mr of 74,000-80,000, and 38,000 after reduction.
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D004072:Digitonin / Q000494:pharmacology; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008213:Lymphocyte Activation; D008970:Molecular Weight; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007135:Immunoglobulin Variable Region; D011948:Receptors, Antigen, T-Cell; D004072:Digitonin