Establishment of a human T cell hybridoma cell line producing suppressor factor specific for anti-thyroglobulin antibody production.
Thyroglobulin (Tg)-binding peripheral blood T cells from a normal individual were fused with a T cell leukemia cell line (Jurkat-AG9) treated by emetine and actinomycin D. Several cell lines were established from thus-prepared human T cell hybridomas. The culture supernatant from one of these lines (Tg-Ts47) whose phenotype was OKT3- 11+ 4+ 8- suppressed the generation of Tg-specific antibody-forming cells from the lymphocytes of patients with Hashimotos' chronic thyroiditis, but not anti-SRBC and anti-ovalbumin antibody production from both autologous and patient lymphocytes. Tg-Ts47-derived factors also bore Tg antigen-binding sites. The suppressive activity of the supernatants was shown in almost all patients lymphocytes tested. This indicated that the supernatants of Tg-Ts47 line contain a suppressive factor specific for Tg antigen and capable of acting across allogeneic barriers.
D000917:Antibody Formation; D001665:Binding Sites; D002460:Cell Line; D006801:Humans; D006825:Hybridomas; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D013954:Thyroglobulin / Q000276:immunology* / Q000378:metabolism
D013491:Suppressor Factors, Immunologic; D013954:Thyroglobulin