T cell reactivity of conjugates of N-formyl-methionyl-leucyl-phenylalanine and rye-grass pollen allergens.
Conjugates of rye-grass pollen extract and N-formyl-methionyl-leucyl-phenylalanine were prepared using an activated form of the tripeptide. Introduction of the peptide into the extract brought about an extensive reduction of reactivity with grass-pollen-specific IgE, as measured by RAST inhibition. Despite this loss, guinea pig alveolar macrophages and murine splenic macrophages readily presented the conjugates to T lymphocytes specific for grass pollen allergens and caused their proliferation in vitro.
D000818:Animals; D006168:Guinea Pigs; D007073:Immunoglobulin E / Q000032:analysis; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000276:immunology*; D006109:Poaceae; D011058:Pollen / Q000276:immunology*; D012434:Secale; D013601:T-Lymphocytes / Q000276:immunology*
D007073:Immunoglobulin E; D009240:N-Formylmethionine Leucyl-Phenylalanine