Retroviral mediated transfer and expression of human alpha 1-antitrypsin in cultured cells.
Genetic deficiency of alpha 1-antitrypsin in man is a predisposing factor to emphysema and a disorder potentially correctable by somatic gene therapy. A full-length human alpha 1-antitrypsin cDNA was cloned into a retroviral vector and introduced into cells which package the recombinant gene in a retroviral capsule. Cells infected with the recombinant retrovirus express human alpha 1-antitrypsin mRNA and protein. The recombinant protein is glycosylated, secreted and exhibits anti-protease activity against human neutrophil elastase.
D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005818:Genetic Engineering; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis / Q000235:genetics*; D019896:alpha 1-Antitrypsin Deficiency
D011994:Recombinant Proteins; D000515:alpha 1-Antitrypsin; D004247:DNA