Differentiation-associated carbohydrate chain on human hematopoietic cells recognized by Clerodendron trichotomum lectin.
In the screening of hematopoietic cell line cell aggregations, the extract of Clerodendron trichotomum seed was found to aggregate K-562 and KG-1 specifically. In the flow cytometric analysis using FITC-conjugated purified CTL, it was confirmed that CTL recognizes the specific carbohydrate(s) which seem to appear only in the early stages of differentiation of myeloid (KG-1) and erythroid (K-562) cell line cells and erythrocytes. The CTL binding to K-562 cells was decreased by TPA treatment which is known to induce retrodifferentiation of K-562. It is also found that this carbohydrate(s) were shaded with NANA on the differentiated cells. In the erythrocyte, CTL receptor was partially shaded by NANA.
D050260:Carbohydrate Metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
C051213:Clerodendron trichotomum lectin; D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; D013755:Tetradecanoylphorbol Acetate