Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species.
A rapid microprocedure for isolating detergent (sodium N-lauroyl sarcosinate)-insoluble major outer membrane proteins from Haemophilus species produced results qualitatively identical to those obtained with a commonly used preparative isolation procedure. Proteins isolated by both procedures were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after staining with Coomassie brilliant blue R-250. The time for outer membrane protein isolation was substantially reduced with the rapid procedure, allowing a larger number of membrane preparations to be obtained rapidly for routine analysis.
D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006190:Haemophilus / Q000032:analysis*; D006193:Haemophilus influenzae / Q000032:analysis*; D006801:Humans; D012995:Solubility
D001425:Bacterial Outer Membrane Proteins