Reactions of reducing radicals with ribonuclease.
Radiation-induced reactions of hydrated electrons, formate- and ethanol radicals with ribonuclease were studied by pulse radiolysis and by electrophoresis. Initially formate radicals react rapidly and very specifically with the disulphide bonds of ribonuclease. This reaction leads to aggregation by intermolecular S-S-interchange, the process being more effective at pH 4, since formation and decay of S-S-.-radical anions increases with decreasing pH. With high doses additional unreducible aggregates are formed. Radical formation at the positively charged histidine residues seems to be involved. Hydrated electrons do not react as selectively as the formate radicals, but with several sites in native ribonuclease. Thus with low doses unreducible aggregates are formed. Electrophoresis shows that reaction of the electrons causes fragmentation of the peptide chain, when OH-radicals are scavenged. Very weak transient spectra and very little degradation result on reaction of ethanol radicals with ribonuclease.
D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol; D005561:Formates; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D011675:Pulse Radiolysis; D012260:Ribonucleases; D012710:Serum Albumin, Bovine
D005561:Formates; D005609:Free Radicals; C030544:formic acid; D012710:Serum Albumin, Bovine; D000431:Ethanol; D012260:Ribonucleases