Effects of diethyl maleate on non-protein sulfhydryl content and cellular functions of mouse thymocytes in vitro.
The electrophilic compound diethyl maleate (DEM, 10(-5)-10(-3) M) decreased the content of non-protein sulfhydryl groups in mouse thymocytes in vitro and affected mitotic activity, spontaneous migration and viability of the cells. The mitotic activity of mouse thymocytes was most sensitive to DEM. It is probable that the decrease of the cell content of reduced glutathione (GSH) led to a failure of GSH-dependent cellular functions, such as mitosis and migration. Cytosolic supernatants of mouse and rat thymocytes exhibited GSH S-transferase activities on DEM and 1-chloro-2,4-dinitrobenzene as electrophilic substrates.
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008298:Maleates / Q000378:metabolism / Q000633:toxicity*; D051379:Mice; D008808:Mice, Inbred CBA; D008938:Mitosis / Q000187:drug effects; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology
D008298:Maleates; D013438:Sulfhydryl Compounds; D005982:Glutathione Transferase; C014476:diethyl maleate; D005978:Glutathione