Differentiation of creatine kinase MB and IgA-linked BB isoenzymes on electrophoresis.
IgA-linked creatine kinase (CK, EC 2.7.3.2) is a macro CK type 1 isoenzyme that has an identical electrophoretic mobility to CK-MB. Its presence has the potential of causing misdiagnosis of myocardial infarction. Mixing anti-CK-B antiserum with the sample prior to electrophoresis did not unequivocally distinguish between the two isoenzymes. Similarly, anti-human IgG and IgM antibodies were also ineffective. However, the IgA-linked isoenzyme band was removed by anti-human IgA antiserum. While anti-CK-M antibodies did not affect the electrophoretic mobility of IgA-linked CK-BB, the antibody eliminated both the CK-MB and CK-MM bands. Thus, specific anti-IgA and anti-CK-M antibodies may be used to establish the presence of the myocardial isoenzyme.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D003402:Creatine Kinase / Q000276:immunology / Q000302:isolation & purification*; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007527:Isoenzymes; D009203:Myocardial Infarction / Q000175:diagnosis / Q000201:enzymology
D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007527:Isoenzymes; D003402:Creatine Kinase