title: Structural studies on the murine granulocyte colony-stimulating factor.
abstract: Granulocyte-colony stimulating factor (G-CSF) is a glycoprotein hemopoietic growth factor which regulates the production of granulocytes and macrophages. Reversed-phase microbore high-performance liquid chromatography was employed to purify a number of tryptic and Staphylococcus aureus V8 proteinase peptides generated from approximately 400 pmol G-CSF purified from medium conditioned by lungs from mice previously injected with endotoxin. N-Terminal amino-acid sequence analyses were performed on the parent polypeptide and on four tryptic peptides and one Staphylococcus aureus V8 protease peptide, yielding 68 unique amino-acid assignments; this corresponds to approximately 38% of the molecule.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003115:Colony-Stimulating Factors / Q000032:analysis*; D006098:Granulocytes; D006868:Hydrolysis; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis; D014357:Trypsin
chemical_list: D003115:Colony-Stimulating Factors; D010455:Peptides; D014357:Trypsin