Further studies on the mechanism of CFU-S determination--III. CFU-S cell lineage determination is not influenced by GM-CSF, Epo, multi-CSF nor WEHI 3B conditioned medium.
CFU-S proliferation is under the control of factors such as stimulators and inhibitors whereas commitment of CFU-S is controlled by pluripoietins. We have studied the effects in vitro of Epo, GM-CSF, multi-CSF and WEHI 3B conditioned medium in order to elucidate their eventual role on CFU-S proliferation and cell lineage determination. Neither Epo nor GM-CSF are able to induce the entry of CFU-S into cycle. Multi-CSF and WEHI 3B conditioned medium (similar to interleukin 3) stimulate CFU-S into DNA synthesis. However, we were able to show that the stimulator we have studied is different from IL 3. Epo, GM-CSF, multi-CSF and WEHI 3B CM have no effect on CFU-S commitment. Therefore, these four hemopoietic regulators are different from the pluripoietins we have previously described and have no influence on CFU-S determination.
D000818:Animals; D003470:Culture Media; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D007377:Interleukin-3; D008222:Lymphokines / Q000494:pharmacology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA
D003470:Culture Media; D007377:Interleukin-3; D008222:Lymphokines; D004921:Erythropoietin