An ELISA for anti-thymocyte autoantibody in NZB mice. Comparison with the cytotoxic assay.
An ELISA is described for the detection of anti-thymocyte antibody found in the serum of NZB autoimmune mice. This assay avoids the disadvantages of previous assay techniques which require carefully selected complement sources and/or radioactive labelled target cells. Thymocytes from a variety of strains of mice can be used as the antigenic targets, and the plates stored as long as 1 month. The lymphoma line EL4 can be used as the antigenic target in this assay, but it not as sensitive to most serum pools as thymocytes.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D003601:Cytotoxicity Tests, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D013601:T-Lymphocytes / Q000276:immunology*
D001323:Autoantibodies