Purification of human macrophage colony stimulating factor (CSF-1) from medium conditioned by pancreatic carcinoma cells.
Colony Stimulating Factor-1 has been purified to apparent homogeneity from the serum-free medium conditioned by cultured human pancreatic carcinoma cells which had been induced with phorbol myristate acetate. The purification scheme consisted of sequential steps of batchwise adsorption to calcium phosphate gel, adsorption to lentil lectin-Sepharose, binding to immobilized antibodies, hydrophobic interaction chromatography, and reversed-phase high-performance liquid chromatography. The purified glycoprotein was found to have a subunit molecular weight corresponding to the smallest of four species (approximately 40,000, 33,000, 28,000 and 23,000) which were observed when less purified preparations were examined.
D001483:Base Sequence; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003115:Colony-Stimulating Factors / Q000302:isolation & purification*; D003470:Culture Media; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D046911:Macromolecular Substances; D008264:Macrophages; D008970:Molecular Weight; D010190:Pancreatic Neoplasms / Q000032:analysis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D003470:Culture Media; D046911:Macromolecular Substances; D004247:DNA; D013755:Tetradecanoylphorbol Acetate