A rapid ELISA for measurement of antibodies to nucleic acid antigens using UV-treated polystyrene microplates.
Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their capacity for binding to single-stranded DNA, double stranded DNA and various synthetic polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens provides a simple, rapid, and specific ELISA for measuring anti-nucleic acid antibodies. The assay is at least as sensitive as the more complex method of precoating plates with poly(L-lysine). It is useful for detection of anti-DNA antibodies in sera of systemic lupus erythematous patients, as well as in culture fluids of murine and human anti-DNA-secreting hybridomas.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D046508:Culture Techniques / Q000295:instrumentation; D004247:DNA / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D011107:Polylysine; D011119:Polynucleotides / Q000138:chemical synthesis / Q000378:metabolism; D011137:Polystyrenes / Q000528:radiation effects; D014466:Ultraviolet Rays
D000974:Antibodies, Antinuclear; D004277:DNA, Single-Stranded; D011119:Polynucleotides; D011137:Polystyrenes; D011107:Polylysine; D004247:DNA