Aromatase cytochrome P-450. Purification and characterization of the enzyme from human placenta.
Aromatase cytochrome P-450 (P-450arom) was purified from human placental microsomes. Preparations exhibit a single major band of approximately 55 kDa on SDS-polyacrylamide gel electrophoresis and have a specific content of 11-13 nmol P-450/mg protein. The purified enzyme exhibits spectral properties typical of ferric and ferrous forms of cytochromes P-450. Full enzymatic activity can be reconstituted with rabbit liver P-450 reductase, and catalytic characteristics similar to aromatase in microsomes are observed. Rabbit antibodies to purified P-450arom were affinity purified and show high specificity and sensitivity on immunoblots.
D000906:Antibodies / Q000302:isolation & purification; D001141:Aromatase / Q000032:analysis / Q000302:isolation & purification*; D002384:Catalysis; D005260:Female; D006801:Humans; D008861:Microsomes / Q000201:enzymology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013053:Spectrophotometry; D013379:Substrate Specificity
D000906:Antibodies; D001141:Aromatase