Effect of 4-hydroxynonenal on c-myc expression.
The 4-hydroxynonenal aldehyde (HNE), a product of lipid peroxidation with high biological activity, inhibits cancerous growths in vivo and in vitro. The mechanism by which this aldehyde acts is not yet understood. The c-myc oncogene seems to be involved in the regulation of cellular multiplication and transformation. We evaluated the c-myc expression and the RNA, DNA and protein synthesis in K562 cells. These cells were incubated for 1 hour in presence of several aldehyde concentrations (range 5.10(-7) to 10(-4)), then washed and kept for 20 hours in a growth medium until used. HNE inhibited both the nucleic acids and protein synthesis in a dose dependent manner, and c-myc expression was evaluated in the K562 cells after incubation with 10(-4) M or 10(-6) M HNE. HNE inhibited c-myc expression only at the highest dose. These preliminary results may suggest that the inhibition of c-myc expression is related to nucleic acid synthesis inhibition following HNE exposure.
D000447:Aldehydes / Q000494:pharmacology*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D007951:Leukemia, Myeloid; D009363:Neoplasm Proteins / Q000096:biosynthesis; D011519:Proto-Oncogenes / Q000187:drug effects*; D012334:RNA, Neoplasm / Q000096:biosynthesis
D000447:Aldehydes; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C027576:4-hydroxy-2-nonenal