Fibronectin is present on B-cells but not on OKT 3-positive T-lymphocytes or Leu 11-positive natural killer cells.
We examined T-cells, B-cells, and natural killer cells of four normal individuals for surface-bound fibronectin (FN) using fluorescence-activated cell sorter analysis and double fluorescence labeling with monoclonal antibodies. While Leu 12 (CD 19)-positive B-cells stained also uniformly with anti-FN antibody, neither OKT 3 (CD 3)-positive T-cells nor Leu 11 (CD 16)-positive natural killer cells could be labeled with anti-FN. Although an anti-FN receptor antibody was not available, these data strongly suggest a distinct pattern of FN-binding by human lymphocytes.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000032:analysis*; D002469:Cell Separation; D005353:Fibronectins / Q000032:analysis* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000032:analysis*; D013601:T-Lymphocytes / Q000032:analysis* / Q000145:classification
D000911:Antibodies, Monoclonal; D005353:Fibronectins