Use of 'nuclear monolayers' to identify factors influencing DNA double-strand breakage by X-rays.
Nuclear monolayers, prepared by treatment of mammalian cells with non-ionic detergents, showed increased sensitivity to X-ray-induced DNA double-strand breakage (dsb), as compared with intact cells, due to a decrease in the low-dose 'shoulder'. The DNA dsb dose-response shoulder could be restored by irradiating nuclei in the presence of sulphydryl compounds. However, the ineffectiveness of glutathione, when used at near cellular levels, in restoring the shoulder, suggested a possible role for protein sulphydryls in the radiation response of intact cells.
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D006224:Cricetinae; D004247:DNA / Q000528:radiation effects*; D004072:Digitonin / Q000494:pharmacology; D005978:Glutathione / Q000502:physiology; D051379:Mice; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology
D011092:Polyethylene Glycols; D017830:Octoxynol; D004247:DNA; D005978:Glutathione; D004072:Digitonin