Deoxythymidine and deoxycytidine incorporation into DNA in B leukemic cells.
Measurements of radioactive DNA after incubation of normal and B leukemic peripheral mononuclear cells, from c-ALL and CLL with labeled deoxythymidine (dTh) and deoxycytidine (dCt) showed that for dTh, incorporation into DNA was similar for normal and c-ALL cells but lower in B-CLL cells and that for dCt, incorporation was highest in c-ALL and lowest in CLL cells. These results contrast with those of dTh and dCt kinase activities; the former has been previously found elevated in c-ALL cells, and the latter is found, in the present study, similar in the three groups tested.
D001402:B-Lymphocytes / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D003841:Deoxycytidine / Q000378:metabolism*; D003842:Deoxycytidine Kinase / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000097:blood*; D010641:Phenotype; D013936:Thymidine / Q000378:metabolism*
D003841:Deoxycytidine; D004247:DNA; D003842:Deoxycytidine Kinase; D013936:Thymidine