[Comparison of the effect of the epidermal growth factor (EGF) and its cyanogen bromide derivative on EGF receptor phosphorylation and uridine uptake in a cell culture].
In contrast to the intact EGF, a cyanogen bromide derivative of EGF (EGF-CNBr) does not induce an increase in uridine phosphorylation rate in 3T3 cells, the ability of the EGF-CNBr to stimulate autophosphorylation of the EGF-receptor in A-431 cells being reserved. EGF and EGF-CNBr were used in concentrations promoting their equivalent binding with EGF receptor in both the series of experiments, which was necessary because of a decreased affinity to binding EGF-CNBr. Thus, the EGF-induced receptor autophosphorylation was not enough for uridine kinase activation. The differences between EGF and EGF-CNBr cellular processing made it possible to discuss potential ways of uridine-kinase activity regulation during the early period of stimulation of quiescent cell cultures.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003488:Cyanogen Bromide / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D051379:Mice; D010766:Phosphorylation; D011494:Protein Kinases / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D014529:Uridine / Q000378:metabolism*; D014541:Uridine Kinase / Q000378:metabolism
D004815:Epidermal Growth Factor; D011494:Protein Kinases; D014541:Uridine Kinase; D066246:ErbB Receptors; D003488:Cyanogen Bromide; D014529:Uridine
Sravnenie vliianiia épidermal'nogo faktora rosta (EFR) i ego tsian-bromidnogo proizvodnogo na fosforilirovanie retseptora EFR i postuplenie uridina v kul'ture kletok.