Biochemical studies on a cell surface determinant involved in T-cell proliferation.
Previous studies have shown that a monoclonal antibody (TH5.2) recognizes a cell-surface determinant which is involved in the proliferative capability of T cells. The work reported here demonstrates that the T-cell-surface antigen recognized by TH5.2 is a glycoprotein of 55,000 to 60,000 molecular weight. The molecule shows a single molecular weight species upon reduction and denaturation, and it contains only a few percent of tunicamycin-sensitive carbohydrate structures. As shown in sequential immunoprecipitation studies, the TH5.2 antigen is on a molecule distinct from the interleukin-2 (Tac) receptor and the T4 molecule. Cell-surface antigenic modulation experiments indicate that the TH5.2 antigen does not comodulate with, and therefore is distinct from, the T3, T4, T8, and Leu-5 T-cell antigens.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification*; D002460:Cell Line; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology*; D014415:Tunicamycin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006023:Glycoproteins; D008565:Membrane Proteins; D014415:Tunicamycin