The use of interleukin 1+ and interleukin 1- cell lines to dissociate signals involved in the induction of cytolytic T cells.
The availability of paired homogeneous Ia+ tumour cell lines (P388AD, interleukin (IL)-1+, and P388NA, IL-1-), which differ in inducibility for IL-1, provided a unique opportunity to assess directly the role of Ia and IL-1 in the induction of cytolytic T cells (CTL) to trinitrophenol (TNP)-modified self. TNP-P388AD but not TNP-P388NA consistently induced H-2-restricted, hapten-specific CTL. However, in the presence of an exogenous source of IL-1, TNP-P388NA was able to induce CTL of the magnitude seen with TNP-P388AD. Both Ia expression and IL-1 secretion were necessary in that when TNP-modified purified T cells were utilized as stimulators, CTL activity was not demonstrated even if IL-1 was added, but was only seen when unmodified, P388AD, or spleen cells were added to the cultures.
D000818:Animals; D002460:Cell Line; D005260:Female; D006241:Haptens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007375:Interleukin-1 / Q000502:physiology*; D007941:Leukemia P388 / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D006241:Haptens; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D014301:Trinitrobenzenes