title: Corticotrophin-releasing factor in extra-hypothalamic brain of the mouse: demonstration by immunoassay and immunoneutralization of bioassayable activity.
abstract: Corticotrophin-releasing factor (CRF) bioactivity has been described in the extra-hypothalamic brain, but its relationship to hypothalamic CRF has remained questionable. Of the seven regions of the mouse brain examined, highest concentrations of CRF-like immunoreactivity (CRF-LI) and bioassayable CRF activity were present in the median eminence and hypothalamus. However, substantial CRF-LI and bioassayable CRF activity were also seen in brain extracts from the amygdala, thalamus, frontal cortex, pons medulla and cerebellum. Bioactivity was largely neutralized by prior incubation with heat-inactivated antiserum to ovine CRF. These findings, in conjunction with previous immunocytochemical evidence, strongly suggest that a substance closely resembling hypothalamic CRF is present in the extrahypothalamic brain of the mouse.
mesh_terms: D000679:Amygdala / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002531:Cerebellum / Q000032:analysis; D002540:Cerebral Cortex / Q000032:analysis; D003346:Corticotropin-Releasing Hormone / Q000032:analysis*; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D008473:Median Eminence / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011149:Pons / Q000032:analysis; D013788:Thalamus / Q000032:analysis
chemical_list: D003346:Corticotropin-Releasing Hormone