In vivo administration of lymphocyte-specific monoclonal antibodies in nonhuman primates. Delivery of ribosome-inactivating proteins to spleen and lymph node T cells.
The selective delivery in vivo of a T lymphocyte-specific monoclonal antibody and immunotoxin conjugates to T cells in lymph node and spleen was assessed in rhesus monkeys. A transient coating of all T lymphocytes in the lymph nodes and spleens of healthy rhesus monkeys could be achieved after infusion of unconjugated anti-T11. Because derivatized antibody is cleared more rapidly than unconjugated antibody, it was necessary to infuse a higher dose of immunotoxin than antibody alone to achieve saturation of the lymphocyte binding sites with anti-T11. When sufficient antibody-toxin conjugate was infused, toxin was readily demonstrable on lymph node and spleen T cells by 16 h after infusion. This demonstration that toxins can be successfully delivered with specificity to target T cell populations in the monkey suggests that killing of restricted cell populations in vivo should be feasible.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000032:analysis / Q000276:immunology; D000941:Antigens / Q000276:immunology; D000972:Antineoplastic Agents, Phytogenic; D004220:Disulfides; D006651:Histocytochemistry; D000922:Immunotoxins; D008198:Lymph Nodes / Q000166:cytology*; D008253:Macaca mulatta; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000008:administration & dosage* / Q000032:analysis; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D000972:Antineoplastic Agents, Phytogenic; D004220:Disulfides; D000922:Immunotoxins; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum; D009699:N-Glycosyl Hydrolases; D000076985:Saporins