Interaction between the gene 5 protein, gene 5 protein/single stranded fd DNA complex and gene 8 protein of the filamentous phage fd.
An affinity column consisting of gene 8 protein, the major coat protein of fd phage, bound to Sepharose was prepared. Isolated gene 5 protein/single stranded fd DNA complex was found to bind to this column and was eluted with fd phage single stranded fd DNA. pH changes, and 1 M CaCl2 were not effective in eluting the protein from the affinity column. Gene 5 protein/single stranded fd DNA complex from the crude extracts of fd-infected E. coli also bound to the column, as did isolated gene 5 protein; whereas fd single stranded DNA alone did not. These results may be relevant for the illucidation of the molecular events occurring in the early stages of fd phage assembly.
D001120:Arginine / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D002213:Capsid / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002846:Chromatography, Affinity; D003090:Coliphages / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D008970:Molecular Weight; D012685:Sepharose; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000378:metabolism*
D002250:Carbon Radioisotopes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012685:Sepharose; D001120:Arginine; D002122:Calcium Chloride; D013936:Thymidine