title: Inhibition of protein synthesis does not block physiological responses to odors in the frog over the short term.
abstract: Frog olfactory mucosae were stimulated with an odorant every 60 sec and electrophysiological responses (electroolfactograms or EOGs) were monitored continuously--before, during, and after treatment with puromycin, a protein synthesis inhibitor. Repeated administration of puromycin at 4 hr intervals over a 36 hr period failed to suppress EOG responses. In an alternative approach, olfactory responses were inhibited with ethyl bromoacetate, a vaporous alkylating agent. EOG responses failed to reappear over a 72 hr period. We conclude that receptor turnover is not readily influenced by stimulation, and that the turnover time of frog olfactory receptor proteins is of the order of days or longer.
mesh_terms: D000818:Animals; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008465:Mechanoreceptors / Q000187:drug effects / Q000502:physiology; D009297:Nasal Mucosa / Q000187:drug effects / Q000502:physiology*; D009812:Odorants; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology*; D011894:Rana pipiens
chemical_list: D011691:Puromycin; D007930:Leucine