Concentration-dependent effect of met-enkephalin on human polymorphonuclear leukocytes.
Met-enkephalin in 10(-9)-10(-7)M concentrations exerts an ADCC-stimulating effect on human PMNLs through naloxone-sensitive opiate receptors, elevating the cytoplasmic free Ca2+ and cGMP levels. In higher, 10(-6)-10(-5)M concentrations ME has a cAMP-elevating effect and a rapid 45Ca2+ influx was observed. This latter effect of ME, which is also associated with the suppression of ADCC activity, was abolished by the enkephalinase inhibitor, puromycin. A strong relationship is suggested between the suppressing effect of metenkephalin, enkaphalinase and the protein kinase C system.
D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D002118:Calcium / Q000097:blood; D003600:Cytosol / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D011691:Puromycin / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology
D014268:Trifluoperazine; D011691:Puromycin; D004744:Enkephalin, Methionine; D002118:Calcium