Immunohistochemical localization of types I and III collagen and fibronectin in the dentine of carious human teeth.
Reparative dentine formed as a response to caries was mostly type I collagen similar to that of normal dentine. The predentine related to reparative dentine reacted positively with antisera to types I and III collagen and to fibronectin. Normal odontoblasts and their processes reacted positively both with types I and III collagen antibodies. Fibronectin was related to odontoblasts and their processes pericellularly. Odontoblasts appeared not to lose totally their developmental ability to synthesize type III-like molecules after maturation. Pulp fibroblasts reacted positively both with types I and III antibodies as well as antifibronectin. The cell-free and cell-rich zones revealed a dense layer of fibres reacting positively with type III collagen and fibronectin antibodies. The width of these zones were reduced in relation to reparative dentine.
D000293:Adolescent; D000328:Adult; D003094:Collagen / Q000378:metabolism*; D003731:Dental Caries / Q000378:metabolism* / Q000473:pathology; D003782:Dental Pulp / Q000473:pathology; D003804:Dentin / Q000378:metabolism* / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry
D005353:Fibronectins; D003094:Collagen