Non-selective inhibition of mammalian protein kinases by flavinoids in vitro.
In vitro, the flavinoids quercetin, morin, and rutin produced comparable inhibition of protein kinase C prepared from bovine brain, the epidermal growth factor (EGF)-stimulated tyrosine specific protein kinase associated with A431 cell membranes, and the cAMP-stimulated protein kinase activity associated with rat brain tubulin. Concentrations producing 50% inhibition (IC50's) were in the 10 microM range. These findings demonstrate the lack of specificity of the flavinoids toward cyclic nucleotide-dependent vs cyclic nucleotide-independent protein kinases.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D047428:Protein Kinase Inhibitors
D005419:Flavonoids; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; D004815:Epidermal Growth Factor; D000255:Adenosine Triphosphate; D011493:Protein Kinase C