title: The production of prostaglandin and the regulation of cell division in neonate rat primary mixed glial cultures.
abstract: The production of prostaglandins has been studied in neonate rat primary mixed glial cultures. A correlation was found between inhibition of [3H]thymidine incorporation in the cultures and production of prostaglandin, which was stimulated by the addition of supernatant from Con A-activated rat splenocytes. Inhibition of prostaglandin synthesis in the cultures by indomethacin results in a striking increase in incorporation of [3H]thymidine into the cultures, an effect which was reversed by the addition of exogenous PGE2, but not PGF2 alpha. PGE was the principal prostaglandin detected, with both macrophages/microglia and astrocytes contributing to the output. A possible role for prostaglandins in the modulation of inflammatory responses in the central nervous system is discussed.
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism / Q000502:physiology; D051379:Mice; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains
chemical_list: D008222:Lymphokines; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D003208:Concanavalin A; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin