Effects of eugenol on polymorphonuclear cell migration and chemiluminescence.
In this study, the effects of eugenol on human polymorphonuclear (PMN) cell migration and chemiluminescence were examined in vitro. Utilizing zymosan-activated serum or crude Bacteroides sonicate fractions as chemotractants, we found that eugenol inhibits PMN migration at 6.6 X 10(-2) to 6.6 X 10(-5) mol/L (P less than 0.05). Also, similar effects were observed in PMNs pre-incubated in eugenol. Regardless of concentration, eugenol was not found to induce chemotaxis of PMNs. An examination of PMN membrane activation through chemiluminescence gave results consistent with the chemotaxis data, demonstrating a decrease in light emission at concentrations as low as 6.6 X 10(-6) mol/L (P less than 0.05). In view of these data, the potential effect of eugenol on in vivo (sulcular or periapical) PMN function deserves further study.
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D005054:Eugenol / Q000494:pharmacology*; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D010101:Oxygen Consumption; D013053:Spectrophotometry; D015054:Zymosan / Q000494:pharmacology
D005054:Eugenol; D015054:Zymosan