The development of lymphocytes with T- or B-membrane determinants in the lizard embryo.
To study the ontogeny of lymphocytes in the lizard, Chalcides ocellatus antisera were raised in rabbits against thymocytes collected from adult lizards (AATS) or from embryos of stages 40 and 41 (AETS), and against serum immunoglobulins (AGGS). AATS recognized in adult lizards a surface membrane antigenic system (TA) specific to thymocytes and thymus-derived (T-) cells in spleen, and a distinct antigenic entity (Tt) found exclusively on intrathymic lymphocytes. After exhaustive absorption with adult thymocytes, AETS defined a further antigen (TE). On the other hand, AGGS combined with the immunoglobulin (Ig) determinants present in the cytoplasm and surface membrane of lizard presumptive B lymphocytes. Immunofluorescence assays during the development of the T and B lineage showed that TA+ and Tt+ thymocytes increased from 35% at stage 37 to 96% at stage 41, and were exported precociously to the embryonic splenic environment. Embryonic thymocytes were shown to totally lack surface Ig, whereas 40-50% of splenocytes carried cytoplasmic and/or surface membrane Ig determinants. The data revealed further the presence of a subset of splenocytes bearing the specific markers of both T and B cells.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D005260:Female; D007107:Immune System / Q000196:embryology*; D008116:Lizards / Q000196:embryology* / Q000276:immunology; D008297:Male; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology / Q000196:embryology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology
D000954:Antigens, Surface; D011947:Receptors, Antigen, B-Cell