[The role of the immunoglobin G "hinge" region in heat aggregation and activation of complex-formation].
A high degree of correlation between the capability of subclasses of human immunoglobulins G to form aggregates due to thermal treatment, and their complement-binding activity was established. On the basis of the experimental data obtained by the methods of light scattering, circular dichroism, microcalorimetry, it was supposed that "hinge" region of immunoglobulins G participates in the initial stage of thermal aggregation and in the activation of the process of complement binding.
D001666:Binding Sites, Antibody; D002942:Circular Dichroism; D003166:Complement Activating Enzymes / Q000032:analysis; D003167:Complement Activation; D003172:Complement C1 / Q000032:analysis; D015922:Complement C1q; D006358:Hot Temperature; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis*; D011487:Protein Conformation
D003172:Complement C1; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D015922:Complement C1q; D003166:Complement Activating Enzymes
Rol' "sharnirnoĭ" oblasti immunoglobulinov G v teplovoĭ agregatsii i aktivatsii kompleksoobrazovaniia.