Repair of potentially lethal radiation damage in confluent and non-confluent cultures of human hybrid cells.
The repair of potentially lethal damage (PLDR) in a gamma-irradiated human hybrid cell line (skin fibroblast X HeLa) and its tumourigenic segregant has been studied as a function of cell density at the time of irradiation and during the postirradiation repair period. The data show that PLDR occurs in both non-confluent and confluent cultures of both cell lines. Furthermore, there is evidence that the extent of PLDR is dependent on cell density and that cell-cell contact may be an important factor in this regard.
D002450:Cell Communication; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D004260:DNA Repair; D005347:Fibroblasts; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects*; D013997:Time Factors; D014343:Trypan Blue / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D014357:Trypsin; D014343:Trypan Blue