In vitro studies on leukemia cells and T lymphocytes in hairy cell leukemia.
Hairy cell leukemia cell lines were established from eight untreated patients using purified B cell growth factor (BCGF) in vitro. These cell lines maintained their original cell surface immunophenotype for about 1 month, after which they began to lose one or more of their characteristic surface antigens. The cell lines also maintained typical hairy cell leukemia morphology for 2-3 months in vitro but later showed an increasing number of multinucleate giant cells that maintained a B cell surface phenotype. The cell lines became independent of exogenously provided BCGF after at least 1 month in vitro and secreted BCGF activity into culture supernatants in most cases. Some cell lines also acquired Epstein-Barr virus nuclear antigen positivity after variable period. Two hairy cell leukemia patients also showed hyperactive T cell responses in vitro and exhibited spontaneous T cell proliferation in culture without exogenously supplied interleukin-2. These T cell lines had the T helper phenotype and secreted significant amounts of T cell-associated lymphokines with BCGF and interleukin-2 activity into culture supernatants.
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology; D002455:Cell Division; D019309:Epstein-Barr Virus Nuclear Antigens; D006801:Humans; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology; D007943:Leukemia, Hairy Cell / Q000276:immunology / Q000473:pathology*; D008222:Lymphokines / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007378:Interleukins; D008222:Lymphokines; D015847:Interleukin-4