Role of neutrophils in hemorrhagic shock-induced gastric mucosal injury in the rat.
Gastric mucosal clearance of 51Cr-labeled red blood cells (51Cr-RBC) was measured in rats during a 30-min control period, a 30-min ischemic period (hemorrhage to 27 mmHg arterial pressure), and a 60-min reperfusion period (reinfusion of shed blood). In untreated (control) rats, a dramatic rise in the leakage of 51Cr-labeled red blood cells into the gastric lumen was observed during the reperfusion period. Treatment with neutrophil antiserum attenuated 51Cr-labeled red blood cell flux into the gastric lumen. Using the radioactive microsphere technique, neutrophil-depleted animals were shown to have higher blood flows in the ischemic period than the untreated rats. Bleeding of untreated rats to a mean arterial pressure of 40 mmHg resulted in blood flows that were not different from those in antiserum-treated rats bled to 27 mmHg and leakage of 51Cr-labeled red blood cells similar to that measured in antiserum-treated rats. The results of this study indicate that neutrophils play an important role in hemorrhagic shock-induced gastric bleeding.
D000818:Animals; D001783:Blood Flow Velocity; D005753:Gastric Mucosa / Q000098:blood supply; D006471:Gastrointestinal Hemorrhage / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D007511:Ischemia / Q000209:etiology / Q000503:physiopathology; D008297:Male; D008833:Microcirculation; D009503:Neutropenia / Q000150:complications; D009504:Neutrophils / Q000502:physiology*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012771:Shock, Hemorrhagic / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D013272:Stomach Diseases / Q000097:blood / Q000209:etiology* / Q000503:physiopathology