Plasma interleukin-1 activity in humans undergoing hemodialysis with regenerated cellulosic membranes.
Because of the similarity between the acute phase response and the post-hemodialysis syndrome, it has been proposed that human blood monocytes adhering to the hemodialysis membrane are exposed to several inducers of interleukin-1 (IL-1). These include endotoxin from the dialysate fluid and C5a activated on the surface of regenerated cellulosic hemodialysis membranes. In order to test this hypothesis, we measured IL-1 plasma activity by employing gel filtration to remove IL-1 inhibitors and subsequent augmentation of lectin-induced murine thymocyte proliferation in each fraction. To evaluate the acute response of end stage renal disease patients during a single hemodialysis with a regenerated cellulosic membrane, plasmas were tested before and after hemodialysis. Control plasmas were obtained from healthy individuals. Plasma IL-1 activity eluted at a molecular weight of predominantly 15 kD. This material was neutralized by anti-human-IL-1 which does not recognize IL-2. IL-1 activity was detected in the pre-dialysis plasma of 6 out of 7 patients and rose in 6 patients following a 5 hour hemodialysis. This increase was associated with a significant rise in body temperature. This study supports the hypothesis that hemodialysis with regenerated cellulose might augment IL-1 production in endstage renal disease patients maintained on longterm hemodialysis. It demonstrates for the first time that dialysis patients have detectable plasma levels of IL-1 compared to normal individuals.
D000328:Adult; D001681:Biological Assay; D002482:Cellulose; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis*; D007676:Kidney Failure, Chronic / Q000276:immunology / Q000628:therapy; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects*
D007375:Interleukin-1; D002482:Cellulose