Quantitative in situ hybridization histochemistry reveals increased levels of corticotropin-releasing factor mRNA after adrenalectomy in rats.
A 35S-labeled 48-base synthetic oligonucleotide complementary to a portion of the rat corticotropin-releasing factor (CRF) mRNA was used for in situ hybridization histochemistry. CRF-synthesizing cells were located in the paraventricular nucleus of the hypothalamus. These cells were observed in the medial parvocellular subdivision where there was a 90% increase in the amount of CRF mRNA per unit volume after adrenalectomy.
D000315:Adrenalectomy; D000818:Animals; D001345:Autoradiography; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D006651:Histocytochemistry; D008297:Male; D009693:Nucleic Acid Hybridization; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011910:Rats, Brattleboro; D011919:Rats, Inbred Strains; D013462:Sulfur Radioisotopes
D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D003346:Corticotropin-Releasing Hormone