The response of dog intradental nerves to hypertonic solutions of CaCl2 and NaCl, and other stimuli, applied to exposed dentine.
Responses of single nerve units from the canine and incisor pulps of anaesthetized beagles to CaCl2 (3.5 M, 4.9 M and saturated) and NaCl (2.5 M and 4.0 M) were recorded. The sensitivity of these nerve units to drilling and probing of dentine, and to drying with air blasts, was also studied. Twenty-one out of 22 units responded immediately, either with a few spikes or, sometimes, with a 2-5 s train of impulses, to hypertonic CaCl2 when applied to superficial dentine. Deep in dentine, CaCl2 induced immediately responses in 15 out of 21 units. There were responses to hypertonic NaCl in 15 out of 19 units, but only when applied deep in dentine. This firing had a latency of 15-300 s (mean 94 s) and continued until the solution was washed away. Units sensitive to CaCl2 also responded to drilling, probing and drying. When applied to the exposed pulp, CaCl2 never induced nerve activity, but hypertonic NaCl induced responses in all units tested (n = 19); the latencies were 0-300 s (mean 34 s). The mechanism of nerve activation in response to hypertonic CaCl2 is probably hydrodynamic, and common to several other stimuli as in drilling, probing and air drying. Responses to hypertonic NaCl may have been induced by a direct excitatory effect of Na+-ions on the nerve endings or axons in the pulp-dentine border.
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology*; D003481:Cuspid; D003804:Dentin / Q000187:drug effects / Q000294:innervation*; D004285:Dogs; D005260:Female; D006982:Hypertonic Solutions; D007180:Incisor; D008297:Male; D009411:Nerve Endings / Q000187:drug effects*; D009997:Osmotic Pressure; D012965:Sodium Chloride / Q000494:pharmacology*
D006982:Hypertonic Solutions; D012965:Sodium Chloride; D002122:Calcium Chloride