Effects of serum immunosuppressive factors on antitumor cytotoxicity of interleukin 2-induced lymphocytes.
The cytotoxic ability of interleukin 2 (IL2)-induced T cells and the effect on their cytotoxicity of serum suppressive factors obtained from cancer patients were examined. Peripheral blood mononuclear cells (PBL) of healthy adults were IL2-cultured for 72 h either together with a gastric carcinoma cell line MKN-28 or with phytohemagglutinin, then IL2-cultured for another 11 days. Cytotoxicity of the MKN-28 precultured lymphocytes against MKN-28 was particularly increased. Presence during preculture or during cytotoxicity assay of sera from gastric cancer patients having the same histological type as MKN-28 suppressed the cytotoxicity. However, the serum suppressive effect on cytotoxicity of the IL2-cultured lymphocytes was far milder than that on PBL cytotoxicity.
D000230:Adenocarcinoma / Q000276:immunology; D000328:Adult; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors
D006023:Glycoproteins; D007376:Interleukin-2; D009363:Neoplasm Proteins; D010835:Phytohemagglutinins; C038588:serum immunosuppressive factor, human