Cellular progesterone receptor phosphorylation in response to ligands activating protein kinases.
Progesterone receptors were immunoprecipitated with monoclonal antibodies KD68 from lysates of human breast carcinoma T47D cells labelled to steady state specific activity with 32Pi. The 120 kDa 32P-labelled progesterone receptor band was resolved by polyacrylamide gel electrophoresis and identified by autoradiography. Phosphoamino acid analysis revealed serine phosphorylation, but no threonine or tyrosine phosphorylation. Treatment of the 32Pi-labelled cells with EGF, TPA or dibutyryl cAMP had no significant quantitative effect on progesterone receptor phosphorylation, though the EGF receptor and the cAMP-dependent protein kinases have been reported to catalyze phosphorylation of purified avian progesterone receptor preparations in cell free systems. Progesterone receptor phosphorylation on serine residues was increased by 2-fold in cells treated with 10 nM progesterone; EGF had no effect on progesterone-mediated progesterone receptor phosphorylation.
D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000378:metabolism; D002460:Cell Line; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006801:Humans; D007700:Kinetics; D008024:Ligands; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011374:Progesterone / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D008024:Ligands; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D011980:Receptors, Progesterone; D011374:Progesterone; D004815:Epidermal Growth Factor; D011494:Protein Kinases