Effect of transforming growth factor beta (TGF-beta) on ATP citrate lyase in isolated hepatocytes.
Transforming growth factor beta (TGF-beta) activates ATP citrate lyase in freshly isolated rat liver hepatocytes in a time dependent manner. Maximal stimulation of the enzyme occurred with less than thirty minutes of incubation of the cells with TGF-beta. The half-maximal effect on the enzyme determined in hepatocytes incubated with TGF-beta for 10 min at 37 degrees C was elicited by TGF-beta concentrations in the 10-11 - 10-12 M range. The potential role of TGF-beta stimulation of ATP citrate lyase activity in new membrane synthesis is discussed.
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004789:Enzyme Activation; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D009363:Neoplasm Proteins / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D015291:Transforming Growth Factors
D009363:Neoplasm Proteins; D010455:Peptides; D015291:Transforming Growth Factors; D001275:ATP Citrate (pro-S)-Lyase