Relationship between primary tumor volume and lung metastasis in Lewis lung carcinoma (1). Assay for time course of development of metastasis.
Relationship between primary tumor volume and development of lung metastases was investigated in Lewis lung carcinoma in male C57BL/6 mice. Tumor cells (1-3 X 10(5] were injected into the muscle of the right hind legs and growth curves of primary tumors were obtained. The time course of development of metastases was determined from the size of lung colonies at autopsy by using growth rate of lung colonies. The first development of metastasis was 4 to 7 days after injection. The incidence of metastases increased as a function of time and was presented by Gompertz function as in primary tumor volume. Therefore, a linear relationship was found to exist between the incidence of metastases and the primary tumor volume. This assay is considered to be useful to understand the time course of development of metastases without resection of the primary tumors.
D000818:Animals; D002277:Carcinoma / Q000473:pathology / Q000556:secondary*; D002460:Cell Line; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000473:pathology*; D013997:Time Factors