Interleukin-1 secretion by peripheral blood monocytes and synovial macrophages from patients with rheumatoid arthritis.
Blood monocytes and synovial fluid and tissue macrophages were examined for their ability to produce interleukin-1 (IL-1) measured in a mouse thymocyte proliferation assay. Spontaneous production of IL-1 by monocytes from patients with rheumatoid arthritis (RA) or ankylosing spondylitis was higher than that by cells from normal subjects, patients with osteoarthritis or patients with RA treated with gold. IL-1 production in response to LPS stimulation was similar in all groups. Spontaneous IL-1 production by synovial fluid macrophages from patients with RA was similar to that of their monocytes, but the response to LPS was smaller. Synovial tissue macrophages produced little IL-1. Similar results were obtained in assays of fibroblast proliferation.
D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000276:immunology*; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use; D006046:Gold / Q000627:therapeutic use; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology*; D010003:Osteoarthritis / Q000276:immunology; D013167:Spondylitis, Ankylosing / Q000188:drug therapy / Q000276:immunology; D013582:Synovial Fluid / Q000166:cytology; D013583:Synovial Membrane / Q000473:pathology
D005938:Glucocorticoids; D007375:Interleukin-1; D006046:Gold