Characterization of beta-lactamases in situ on polyacrylamide gels.
An inhibitor-based characterization system which allowed the identification of beta-lactamases after isoelectric focusing on polyacrylamide gels was developed. This system, using potassium clavulanate and oxacillin, distinguished type I chromosomally mediated enzymes from other beta-lactamases of gram-negative bacteria.
D002969:Clavulanic Acids; D003023:Cloxacillin; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics; D007525:Isoelectric Focusing; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
D002969:Clavulanic Acids; D001618:beta-Lactamases; D003023:Cloxacillin