Effect of macrophage colony-stimulating factor on the function of resident peritoneal macrophages.
Resident peritoneal cells of monocyte/macrophage lineage were collected by lavage of mice and incubated in vitro for 1-3 d in a culture medium containing various concentrations of macrophage colony-stimulating factor (M-CSF). Stimulation of the cells by zymosan showed that the potency of producing luminol-dependent chemiluminescence had been markedly increased in the CSF-treated cells, indicating increased generation of active oxygen species in these cells. There was an optimal concentration of M-CSF for the enhancement, and the potency of the cells was notably decreased by an overdose of M-CSF. The result was interpreted as being due to the down-regulation of M-CSF receptor.
D000818:Animals; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000502:physiology* / Q000652:urine; D008163:Luminescent Measurements; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010537:Peritoneum / Q000166:cytology; D015054:Zymosan / Q000494:pharmacology
D007377:Interleukin-3; D015054:Zymosan