Uracil-DNA glycosylase activity in human blood cells.
We studied uracil-DNA glycosylase activities systematically in all types of human peripheral blood cells. The highest amounts of uracil-DNA glycosylase activity were found in cells capable of using their genetic information either in DNA replicative or repair synthesis or in DNA transcription. These cells included cytotoxic/suppressor and inducer/helper T lymphocytes, B lymphocytes and monocytes. On the other hand, the peripheral blood mature end cells, erythrocytes, platelets and granulocytes, contained very little if any uracil-DNA glycosylase activity. In addition to this biological capacity, we show that the housekeeping excision repair capacity of uracil-DNA glycosylase is well maintained in human peripheral blood mononuclear cells throughout life from the neonatal period to old age.
D000328:Adult; D000367:Age Factors; D000368:Aged; D001402:B-Lymphocytes / Q000201:enzymology; D001792:Blood Platelets / Q000201:enzymology; D045647:DNA Glycosylases; D004260:DNA Repair; D004912:Erythrocytes / Q000201:enzymology; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007231:Infant, Newborn; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology; D009699:N-Glycosyl Hydrolases / Q000097:blood*; D013601:T-Lymphocytes / Q000201:enzymology; D051981:Uracil-DNA Glycosidase
D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase