Radiation-induced alterations in binding of concanavalin A to cells and in their susceptibility to agglutination.
Cell susceptibility to agglutination mediated by a plant lectin, concanavalin A (Con A), and the binding capacity of Con A to cells following gamma-irradiation have been examined in mouse myeloid leukaemia cells cultured in suspension. Irradiation caused an immediate decrease in the amount of Con A bound to the cell surface, whereas susceptibility of irradiated cells to agglutination by Con A was unchanged when compared to that of the unirradiated cells. Post-irradiation incubation of cells at 37 degrees C resulted in a temporary, more than 1.3-fold increase in cell susceptibility to agglutination 60 min after irradiation, whereas binding capacity of cells for Con A gradually recovered following irradiation, reaching a comparable level to that of unirradiated cells 3 h after irradiation. Cell susceptibility to agglutination by Con A does not depend strongly on its binding capacity.
D000371:Agglutination / Q000187:drug effects / Q000528:radiation effects*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D003208:Concanavalin A / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000473:pathology; D051379:Mice
D003037:Cobalt Radioisotopes; D003208:Concanavalin A