title: Comparison of phenotypic and genotypic features in human primary large bowel carcinomas and lymph node metastases.
abstract: In order to test the contention that metastasis is a selective process and that therefore metastases might show a more restricted pattern of phenotypic and genotypic characteristics than primary tumors, we compared the expression of carcinoembryonic antigen, Ca 19-9, secretory component, serotonin, and mucin production as well as flow cytometric data on DNA content and percentage of S-phase cells in 87 primary large bowel carcinomas and their lymph node metastases. In a majority of the cases primary tumors and their metastases were largely identical with regard to the examined phenotypic features. In discrepant cases, however, metastases did not invariably show a more restricted pattern than primary tumors, indicating high differentiational plasticity of primary and metastatic colorectal cancer cells. In contrast, in a number of cases genotypic discrepancies were observed. We conclude that phenotypic characteristics of colorectal cancer cells cannot be used to study the pathogenesis of lymph node metastasis. Genotypic studies, however, suggest that lymphogenic metastasis may be a selective event.
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis; D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen / Q000032:analysis; D003110:Colonic Neoplasms / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005838:Genotype; D006801:Humans; D008207:Lymphatic Metastasis; D009077:Mucins / Q000096:biosynthesis; D010641:Phenotype; D012004:Rectal Neoplasms / Q000032:analysis*; D012635:Secretory Component / Q000032:analysis; D012701:Serotonin / Q000032:analysis
chemical_list: D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen; D004273:DNA, Neoplasm; D009077:Mucins; D012635:Secretory Component; D012701:Serotonin