Enhancement of testicular cysteine proteinase inhibitor level in vitamin E-deficient rats.
The effect of vitamin E deficiency on levels of proteinase inhibitors in sex glands of male rats was studied. Inhibitor levels against cysteine proteinases, such as ficin and cathepsin H, and against serine proteinase such as trypsin were examined. Vitamin E deficiency for 4 mo after weaning induced a fivefold increase in cysteine proteinase inhibitor level in testis, a two- to fourfold increase in prostate and epididymis and no change in seminal vesicle. No appreciable change was observed in trypsin inhibitor level in testis, epididymis or seminal vesicle. Therefore, vitamin E deficiency was reflected most sensitively by the cysteine proteinase inhibitor level in testis. These observations agree with our previous findings that alpha-cysteine proteinase inhibitors in serum increased greatly whereas trypsin inhibitor in serum did not change in vitamin E-deficient rats. Major histological changes were observed in the testes of rats fed a vitamin E-deficient diet for 4 mo, although testis weight was not significantly affected by vitamin E deficiency.
D000818:Animals; D056655:Cathepsin H; D002403:Cathepsins / Q000037:antagonists & inhibitors; D003546:Cysteine Endopeptidases; D015853:Cysteine Proteinase Inhibitors; D004822:Epididymis / Q000378:metabolism; D005361:Ficain / Q000037:antagonists & inhibitors; D008297:Male; D011467:Prostate / Q000378:metabolism; D011480:Protease Inhibitors / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D012669:Seminal Vesicles / Q000378:metabolism; D015842:Serine Proteinase Inhibitors; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D014361:Trypsin Inhibitors / Q000378:metabolism; D014811:Vitamin E Deficiency / Q000378:metabolism* / Q000473:pathology
D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D014361:Trypsin Inhibitors; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056655:Cathepsin H; C534128:Ctsh protein, rat; D005361:Ficain