Increased susceptibility to cephamycin-type antibiotics of methicillin-resistant Staphylococcus aureus defective in penicillin-binding protein 2.
A methicillin-resistant strain of Staphylococcus aureus which produced low-affinity penicillin-binding protein 2' (PBP 2') spontaneously lost PBP 2 when the strain was cultivated at 43 degrees C overnight. At 37 degrees C, the mutant had increased susceptibility to cephamycin-type beta-lactams, which showed high affinity for PBP 4. This result suggests that inhibition of PBP 4, in addition to that of PBP 2, is necessary to kill methicillin-resistant strains.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000172:deficiency*; D002511:Cephalosporins / Q000494:pharmacology; D002513:Cephamycins / Q000494:pharmacology*; D006602:Hexosyltransferases; D008565:Membrane Proteins / Q000032:analysis; D008712:Methicillin; D002267:Muramoylpentapeptide Carboxypeptidase / Q000172:deficiency*; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins; D010405:Penicillinase / Q000172:deficiency; D010458:Peptidyl Transferases; D013211:Staphylococcus aureus / Q000187:drug effects*; D013696:Temperature
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D002511:Cephalosporins; D002513:Cephamycins; D008565:Membrane Proteins; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D010405:Penicillinase; D008712:Methicillin