Frequency analysis of human peripheral blood B cells sensitive to CD2 monoclonal antibody-activated T cells.
Recent studies have demonstrated that appropriate pairs of cluster of differentiation 2 (CD2) monoclonal antibodies (MoAbs) directed against two epitopes on the T11 molecule induced human T-cell activation leading to the production of several lymphokines. Here we report that human peripheral blood B lymphocytes cultured in the presence of the CD2 (D66 + T11) MoAb, together with T lymphocytes and monocytes, secreted larger amount of immunoglobulin (Ig) than when they were incubated in the same culture conditions in the presence of pokeweed mitogen (PWM). We further show that the level of Ig secreted by the progeny of a single responsive B cell is similar in both systems and demonstrate that the increase in the Ig concentration is directly related to the high frequency of B-cell precursors sensitive to CD2 MoAb-activated T cells.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001402:B-Lymphocytes / Q000145:classification*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D008222:Lymphokines / Q000378:metabolism; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D008222:Lymphokines; D011043:Pokeweed Mitogens