IL 1 induction by murine T cell clones: detection of an IL 1-inducing lymphokine.
T cell activation is widely believed to depend on interleukin 1 (IL 1) provided by antigen (Ag)-presenting cells (APC). Because IL 1 is not a constitutive product of APC, we examined the features of its production during the interaction of murine T cell clones and APC. We observed that IL 1 was detectable in supernatants of most myoglobin-specific T cell clones grown with APC and Ag. Two of these T cell clones induced exceptionally high levels of IL 1 in their supernatants, and these same clones demonstrated the unusual restriction to I-Ek, which is a low responder type for sperm whale myoglobin. One of these clones was characterized additionally as to the mechanism of IL 1 induction. This clone rapidly stimulated IL 1 production in the APC population (detectable at 4 hr of co-culture) or in macrophages (M phi) or a M phi-like cell line. IL 1 induction was Ag dependent and H-2 restricted. Induction was radioresistant, both on the part of the T cell and of the IL 1 producer. The IL 1-induction process was attributable to a lymphokine produced by the T cell clone. This lymphokine was distinct from IFN-gamma, TNF and CSF-1 and may account for a principal mechanism of T----APC signalling. The induced IL 1 was the same in size, co-mitogenicity, and pyrogenicity as lipopolysaccharide-induced IL 1.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000276:immunology; D003470:Culture Media; D006023:Glycoproteins / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D007375:Interleukin-1 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009211:Myoglobin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha
D003115:Colony-Stimulating Factors; D003470:Culture Media; D006023:Glycoproteins; D006183:H-2 Antigens; D007375:Interleukin-1; D008222:Lymphokines; D009211:Myoglobin; D014409:Tumor Necrosis Factor-alpha