Role of the macrophage-derived hybridoma growth factor in the in vitro and in vivo proliferation of newly formed B cell hybridomas.
It has been shown recently that monocyte-macrophage cells produce a growth factor (HGF) active on newly formed B cell hybridomas. We have studied the effect of HGF on the proliferation of an HGF-sensitive clone of B cell hybridoma. Results obtained showed that the murine P388D1 cell-derived HGF has a m.w. of 29,000 and an isoelectric point (pI) of 6.2 whereas the human monocyte-derived HGF has a m.w. of 34,000 and a pI of 4.9. The HGF activity was not mediated by interleukin 1 because the two activities could be completely separated by gel filtration. Results obtained in in vivo experiments showed that HGF-sensitive cells are tumorigenic in mice. The finding that the HGF biochemical parameters (m.w. and pI) are similar to the ones of a recently described plasmacytoma growth factor suggests that HGF and the plasmacytoma growth factor are similar and that the HGF sensitivity of SP 2/O myeloma cells is reactivated after fusion with normal B lymphocytes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001201:Ascites / Q000209:etiology; D001402:B-Lymphocytes / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology; D006133:Growth Substances / Q000502:physiology*; D006388:Hemagglutinins / Q000096:biosynthesis; D006801:Humans; D006825:Hybridomas / Q000187:drug effects* / Q000276:immunology / Q000637:transplantation; D015850:Interleukin-6; D008264:Macrophages / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000378:metabolism; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology; D010954:Plasmacytoma / Q000473:pathology*
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D006388:Hemagglutinins; D015850:Interleukin-6