A competitive ELISA technique for the measurement of von Willebrand factor antigen (vWF:Ag) using staphylococcal protein A peroxidase.
A competitive ELISA technique for measurement of von Willebrand factor antigen (vWF:Ag) using Staphylococcal Protein A peroxidase is described. The standard used in this assay is partially purified Factor VIII:C/vWF which has been standardized against a conventional method (electroimmunoassay). The results show a close correlation (correlation coefficient 0.956) as compared to the standard Laurell electroimmunoassay technique. Inter-assay and intra-assay coefficients of variation were less than 5%. The technique is simple to perform and results may be obtained within three hours of specimen collection.
D000941:Antigens / Q000032:analysis* / Q000592:standards; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D006735:Horseradish Peroxidase; D006801:Humans; D012015:Reference Standards; D013205:Staphylococcal Protein A; D014841:von Willebrand Factor / Q000276:immunology*
D000941:Antigens; D013205:Staphylococcal Protein A; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; D006735:Horseradish Peroxidase