Properties of a CCAAT box-binding protein.
NF-Y is a sequence-specific DNA-binding protein that interacts with the conserved Y motif of the major histocompatibility complex class II gene, E alpha. Since it is actually a CCAAT box-binding protein, NF-Y also attaches to other promoters bearing CCAAT sequences; yet, it is neither of the previously described transcription factors, CBP or CTF/NF-1. In this report, we document the cell-type distribution and various biochemical properties of NF-Y. The most important findings are that this protein is ubiquitously distributed, that it is probably a metallo-protein, that it has a protease-resistant DNA-binding domain and that the NF-Y/E alpha-olgo complex seems extremely large (greater than 200kD). These data should prove useful in comparisons of NF-Y with other sequence-specific DNA-binding proteins; they have already provided new insights into NF-Y's structure.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002614:Chelating Agents / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008285:Major Histocompatibility Complex; D051379:Mice / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid
D002614:Chelating Agents; D004268:DNA-Binding Proteins; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen