In vitro translation of cucumoviral satellites. II. CARNA 5 from cucumber mosaic virus strain S and SP6 transcripts of cloned (S)CARNA 5 cDNA produce electrophoretically comigrating protein products.
This report shows that (S)CARNA 5, the satellite of cucumber mosaic virus (CMV) strain S, directs the synthesis of two small proteins in a wheat germ cell-free translation system. The two proteins are produced by the translation of both (S)CARNA 5 purified from CMV virions and the (+) strand purified from double-stranded (S)CARNA 5. In addition, two protein products that comigrate electrophoretically with those above are produced when transcripts synthesized in vitro from full-length (S)CARNA 5 cDNA cloned in the plasmid pSP65 are substituted for (S)CARNA 5 in the translation system. The two proteins therefore must derive from a single (S)CARNA 5 sequence.
D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D009029:Mosaic Viruses / Q000235:genetics*; D011188:Potassium / Q000378:metabolism; D014176:Protein Biosynthesis; D012526:Satellite Viruses / Q000235:genetics*; D014158:Transcription, Genetic; D014675:Vegetables; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000032:analysis
D014764:Viral Proteins; D004247:DNA; D007930:Leucine; D008274:Magnesium; D011188:Potassium