Inhibition of natural killer cell activity by antigen-antibody complexes.
The natural killer (NK) cell activity of human peripheral blood mononuclear cells was found to be inhibited by precipitated tetanus toxoid anti-tetanus toxoid complexes (Te/aTe) as well as soluble Te/aTe. Preincubation of the immune complexes with protein A decreased the inhibition of NK cell activity. When mononuclear cells were preincubated with interferon (IF) or interleukin 2 (Il-2) before incubation with Te/aTe, the immune complex-induced inhibition was decreased, while IF or Il-2 added after incubation with the immune complexes had no effect. Using NK cell-enriched suspensions in a single cell agarose assay, the immune complexes were shown to inhibit NK cell activity by inhibiting the formation of effector/target cell conjugates.
D000936:Antigen-Antibody Complex / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology; D007376:Interleukin-2 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000494:pharmacology; D013745:Tetanus Toxoid / Q000276:immunology
D000936:Antigen-Antibody Complex; D007370:Interferon Type I; D007376:Interleukin-2; D013205:Staphylococcal Protein A; D013745:Tetanus Toxoid