Evaluation of human blood monocytes in erythrophagocytosis assay with anti-Rh antibodies.
Results of the phagocytosis assay with monocytes and erythrocytes sensitized with subclass specific anti-Rh(D) antibodies (IgG1, IgG3, IgG1 + 3) are dependent on the selection of sera used for sensitization. Phagocytic abilities expressed as the percentage of active monocytes as well as the number of interacting red cells requires a certain degree of the sensitization of erythrocytes and is higher if serum contains IgG3 antibodies.
D002448:Cell Adhesion; D004912:Erythrocytes / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D015795:HLA-B8 Antigen; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis; D011961:Receptors, Fc / Q000276:immunology*
D006680:HLA Antigens; D015795:HLA-B8 Antigen; D007074:Immunoglobulin G; D007518:Isoantibodies; C033242:RH-antibodies; D011961:Receptors, Fc