T-lymphocyte colony formation of murine thymocytes in agar contained in glass capillaries.
Optimal growth conditions are presented for a new colony test with mouse thymocytes in agar contained in glass capillaries. The kinetics of colony growth and the dependence from the PHA-, I1-2-, agar- and 2-mercaptoethanol concentration are shown. The colony forming cells are identified as T-lymphocytes by usual morphology and by an indirect immunoperoxidase method using mouse anti-Thy 1.2 antibodies.
D000362:Agar; D000818:Animals; D003114:Colony-Forming Units Assay; D005898:Glass; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology*
D000362:Agar