Selenium and the growth of Haemophilus ducreyi.
One of the growth media in current use for Haemophilus ducreyi comprises Mueller Hinton agar, chocolatised horse blood, serum and IsoVitalex (BBL). For a better understanding of growth factors, attempts were made to simplify this complex medium. The horse blood was replaced by haemin (200 micrograms/ml), the serum by albumin (0.2%), and IsoVitalex was substituted only by L-glutamine 0.01%. Most of the strains grew, but when selenium ions were added in a concentration of 3.25 x 10(-3) micrograms/ml, growth was stimulated and became more luxuriant than growth on conventional media.
D003470:Culture Media; D005973:Glutamine / Q000378:metabolism; D006191:Haemophilus ducreyi / Q000254:growth & development*; D012643:Selenium / Q000378:metabolism*; D013268:Stimulation, Chemical
D003470:Culture Media; D005973:Glutamine; D012643:Selenium