Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line.
We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. From results of Northern- and Southern-blot analyses, we conclude that these cDNAs represent two distinct genes, which we have designated mdm-1 and mdm-2. Using DNAs from a panel of Chinese hamster-mouse somatic cell hybrids together with in situ hybridization protocols for gene mapping studies, we have found that these DM-associated, amplified DNA sequences originate from mouse chromosome 10, region C1-C3. Sequences homologous to mdm-1 and mdm-2 are present in the genomes of several species examined, including that of man.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis; D005347:Fibroblasts / Q000473:pathology; D005784:Gene Amplification; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D012333:RNA, Messenger; D004247:DNA