An antigen present in rat adenocarcinoma and normal colon non-epithelial stroma is a novel Forssman-like glycolipid based on isoglobotetraosylceramide.
A glycolipid with blood group A activity detected in the non-epithelial stroma of normal rat colon but not in epithelial cells (Hansson, G.C., Karlsson, K.-A., and Thurin, J. (1984) Biochim. Biophys. Acta 792, 281-292), was purified to homogeneity from normal rat colon and rat colon adenocarcinoma. Mass spectrometry and 1H-NMR spectroscopy of the intact permethylated derivative and gas chromatography after degradation revealed the structure GalNAc alpha 1----3GAINAc beta 1----3Gal alpha 1----3Gal beta 1----4Glc beta 1----1Cer, with the predominant ceramide containing sphingosine and non-hydroxylated 24:0 fatty acid. This identifies this glycolipid as a novel Forssman-like glycolipid, which is a tumor-associated antigen by definition, since it is not present in the normal rat large intestinal epithelium cells but in rat adenocarcinoma derived from these cells.
D000082:Acetaminophen / Q000031:analogs & derivatives*; D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D015478:Antigens, Heterophile / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002849:Chromatography, Gas; D003106:Colon / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology*; D005577:Forssman Antigen / Q000032:analysis*; D005915:Globosides / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis*; D006028:Glycosphingolipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains
D015478:Antigens, Heterophile; D000951:Antigens, Neoplasm; D005915:Globosides; D006017:Glycolipids; D006028:Glycosphingolipids; D000082:Acetaminophen; C045360:2,6-dimethylacetaminophen; D005577:Forssman Antigen