Interleukin 1-driven secretion of interleukin 2 is highly temperature-dependent.
To investigate the temperature dependence of T lymphocyte activation, we have examined the IL 1-induced secretion of IL 2 by the murine T lymphoma cell line LBRM-33-1A5. During 24-hr incubations, there are modest increases in IL 2 secretion as culture temperatures are increased from 33 degrees to 37 degrees C, but IL 2 secretion declines at higher temperatures. However, the kinetics of IL 2 release during the first 8 hr of culture are highly temperature-dependent. The rate of IL 2 release increases linearly with temperature over the range from 33 degrees to 41 degrees C, demonstrating temperature coefficients (Q10) greater than 70. In contrast, IL 2-promoted proliferation of a continuous T cell line is much less temperature-dependent with Q10 values of less than 4.0.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D007375:Interleukin-1 / Q000502:physiology*; D007376:Interleukin-2 / Q000378:metabolism*; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
D007375:Interleukin-1; D007376:Interleukin-2