Activation of effector cells of experimental allergic encephalomyelitis in Lewis rats: comparison of T-cell lines with primary cultured lymph node cells.
We studied the mechanism of activation of effector cells in experimental allergic encephalomyelitis (EAE) by using T-cell lines (EAE/TL) reactive against guinea pig myelin basic protein generated from in vivo primed lymph node cells (LNC) of Lewis rats. EAE-effector cells are activated in the presence of a specific antigen and antigen-presenting cells (APC) with a compatible Ia antigen. The antigen presentation occurs during the first 18 h. EAE-effector cells cannot be activated by allogeneic stimulator cells, but a nonspecific T-cell mitogen, concanavalin A, can activate the effector cells in the presence of syngeneic as well as allogeneic APC.
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000954:Antigens, Surface / Q000235:genetics; D002417:Cattle; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D010641:Phenotype; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000906:Antibodies; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II