Repeated tandem translocations in a clone and subclones of B16-F10 murine melanoma.
In one clone and three subclones isolated from the F10 line of the B16 mouse melanoma, a family of extraordinarily long marker chromosomes was found. Banding analyses showed that these long markers represented repeated tandem translocations. Most of these markers exhibited only two or three C-bands. Immunofluorescence staining using antikinetochore serum revealed that these markers had either two active kinetochores or one active and one inactive kinetochore. The original clone and one of the subclones were highly unstable with respect to the composition of the markers and to the ability for retaining the markers. The other two subclones were found to be relatively stable. Because all three subclones were derivatives of one clone, which was unstable, our data suggest that stable genomes can be generated from unstable progenitors.
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002999:Clone Cells; D005819:Genetic Markers; D007621:Karyotyping; D008545:Melanoma / Q000235:genetics*; D051379:Mice; D014178:Translocation, Genetic
D005819:Genetic Markers