title: Immunochemical detection of cell cycle synchronization in a human erythroleukemia cell line, K562.
abstract: Cells of the human erythroleukemic line K562 can be induced by manipulation of culture conditions to arrest within the G1 phase of the cell cycle, and subsequently to enter S phase synchronously after release from G1. Cell cultures subjected to serum deprivation and hydroxyurea (HU) treatment demonstrated less than 5% of the cells to be in S phase. Four hours after release from HU, 63% of the cells were in S phase, as detected by immunofluorescent staining. This protocol offers a method for synchronization of K562 cells at the G1/S border and a technique for detection of S-phase cells without the use of radioisotopes or flow cytometry instrumentation.
mesh_terms: D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D004261:DNA Replication; D006651:Histocytochemistry; D006801:Humans; D007399:Interphase; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*