Isolation and amino acid sequence of calcitonin gene related peptide from porcine spinal cord.
Porcine calcitonin gene related peptide (pCGRP) was isolated from spinal cord by HPLC combined with bioassays using the rat vas deferens and the rat heart. The amino acid sequence was determined by microsequencing methods to be; S-C-N-T-A-T-C-V-T-H-R-L-A-G-L-L-S-R-S-G-G-M-V-K-S-N-F-V-P-T-D-V-G-S-E-A- F-NH2. The structure determined was found to be more similar to that of the second human CGRP (beta-CGRP) than that of the first human CGRP (alpha-CGRP). Of the spinal cord peptides, only pCGRP showed strong relaxant activity on the electrically induced contraction of the rat vas deferens and strong enhancing effect on the rat heart rate.
D000595:Amino Acid Sequence; D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D006339:Heart Rate / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009479:Neuropeptides / Q000032:analysis*; D051381:Rats; D013116:Spinal Cord / Q000032:analysis*; D013552:Swine
D009479:Neuropeptides; D015740:Calcitonin Gene-Related Peptide