Increased release of interleukin-1 from mouse peritoneal macrophages in vitro after cisplatin treatment.
The supernatants collected from cis-platin (2 micrograms, 5 micrograms, 10 micrograms, treated macrophage monolayers enhance the proliferation of thymocytes by a submitogenic concentration of con A (1 microgram/ml). The supernatants collected from the untreated macrophage monolayers show a gradual ten fold increase in the interleukin-1 (IL-1) activity during 30 min to 48 h incubation at 37 degrees C. Supernatants collected from macrophage monolayers treated with 2 micrograms/ml of cis-platin show only a marginal increase in IL-1 activity as compared to untreated monolayers. However, compared to controls, 30 to 40 fold increases in IL-1 activity were measured in supernatants collected from the macrophage monolayers incubated with 5, 10 and 20 micrograms/ml cis-platin at 37 degrees C. The IL-1 activity in supernatants collected from macrophage monolayers treated with cis platin and LPS are also compared.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000378:metabolism* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000187:drug effects
D007375:Interleukin-1; D008070:Lipopolysaccharides; D002945:Cisplatin