Macrophage-neutrophil interactions: contrasting effects of the monokines interleukin-1 and tumour necrosis factor (cachectin) on human neutrophil adherence.
Incubation of human neutrophils with recombinant human interleukin-1 alpha (IL-1 alpha) resulted in the suppression of neutrophil adherence. In contrast, similar treatment with recombinant human tumour necrosis factor-alpha (TNF alpha, cachectin) resulted in the enhancement of neutrophil adherence. These contrasting effects were noted as early as 5 min after incubation, and persisted for at least 60 min. Simultaneous addition of these two monokines resulted in intermediate values between suppression by IL-1 and enhancement by TNF alpha. The stimulatory effects of the chemotactic peptide FMLP and the phorbol ester PMA were ameliorated by IL-1 but augmented by TNF alpha. The effects of these monokines on neutrophil adherence were abolished by heating but not by polymyxin B treatment, showing that their modulatory properties were not mediated by endotoxin.
D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007104:Immune Adherence Reaction; D007375:Interleukin-1 / Q000276:immunology*; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D011112:Polymyxin B / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011112:Polymyxin B; D013755:Tetradecanoylphorbol Acetate