Immunobeads test (antibody coated polyacrylamide beads) in the immunological characterization of lymphoproliferative disorders.
Three groups of patients with immunoproliferative disorders (15 multiple myeloma, 11 non-Hodgkin's lymphoma, 21 chronic lymphocytic leukemia) were studied by immunological characterization and compared to a group of 20 normal subjects (controls) using anti-immunoglobulin coated polyacrylamide beads (T-B Quantigen test, QT), erythrocyte rosettes (ER), surface immunoglobulin (SIg), and monoclonal antibodies for T and B cells (OKT3; OKT11; OKT8; OKT4; IaDR); null cells (NC) and double marker (DM) cells were also considered. The values for normal subjects for T-B, NC and DM cells were comparable. Results for the patient groups strikingly differed. There were progressively larger differences between the T and B percentages obtained with different techniques. The largest differences were seen in patients with chronic lymphocytic leukemia and the smallest in multiple myeloma patients; values were intermediate in non-Hodgkin lymphoma. The different findings were related to the number of DM cells (ER+, SIg+ QT+) and the different tests used. The importance of these findings in the diagnostic approach to lymphoproliferative disorders is discussed.
D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000032:analysis / Q000145:classification; D006801:Humans; D007136:Immunoglobulins; D008215:Lymphocytes, Null / Q000032:analysis; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000175:diagnosis*; D008863:Microspheres; D010641:Phenotype; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000032:analysis / Q000145:classification; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D000099166:Polyacrylamides