T cell responses to a Dermatophagoides farinae allergen preparation in allergics and healthy controls.
We have studied possible differences between mite-allergic individuals and healthy controls in their T cell proliferation and T cell surface markers after in vitro stimulation with a Dermatophagoides farinae allergen preparation. No significant differences were found between the two groups in 3H-thymidine incorporation in T cells after 7 days in culture with allergen- and antigen-presenting cells. The frequencies of allergen-reactive T cells determined by limiting dilution were also similar in the two groups. Finally, no differences were found in surface markers on allergen-activated T cells from mite-allergic and healthy blood donors using a variety of monoclonal antibodies reactive with T cell subsets and activation antigens. Our conclusion is that with the D. farinae preparation employed in this investigation and using T cells prepared from peripheral blood no significant differences were seen between mite allergics and controls.
D000485:Allergens / Q000276:immunology*; D002455:Cell Division; D004306:Dose-Response Relationship, Immunologic; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007156:Immunologic Memory; D007231:Infant, Newborn; D007700:Kinetics; D008213:Lymphocyte Activation; D008925:Mites / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000485:Allergens