Pertussis toxin inhibits the action of insulin-like growth factor-I.
Effect of pertussis toxin treatment on the actions of insulin-like growth factor-I (IGF-I) was examined in Balb/c 3T3 cells. In competent cells primed with epidermal growth factor, IGF-I stimulated both calcium influx and DNA synthesis. When these primed competent cells were treated with various concentrations of pertussis toxin for 2 hrs, IGF-I-induced calcium influx and DNA synthesis were inhibited in a similar dose dependent manner. The inhibitory action of pertussis toxin well coincided with the toxin-induced ADP-ribosylation of a 41 K-Da protein. These results suggest a possible involvement of a pertussis toxin-sensitive GTP-binding protein in the action of IGF-I.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin; D013002:Somatomedins / Q000037:antagonists & inhibitors*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D013002:Somatomedins; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D037342:Pertussis Toxin; D002118:Calcium