Constitutive production of colony-stimulating factor by mouse lymphoma cell lines is correlated with granulocytosis in vivo.
The mouse lymphoma cell line Eb, its highly metastasizing variant ESb, and an unrelated metastasizing tumor MDAY-D2, were shown to produce colony-stimulating factor (CSF) constitutively both in vitro and in vivo in ascites. For each tumor, the amounts of CSF produced on a per-cell basis in vitro and in vivo were similar. The findings were substantiated using two different methods for CSF determination, a colony assay and an isotope incorporation test. Elevated levels of CSF in serum of mice with tumors were also found. Examination of blood from tumor-bearing mice revealed that whereas total leukocyte counts remained within the normal range, all three tumors caused a reversal of the normal neutrophil-lymphocyte ratio. The severity of the reversal correlated with the propensity of the tumor to elevate serum CSF rather than with in vitro CSF-producing capacity. Thus, whereas production of CSF in vivo was not related to the ability of the tumors to metastasize, it could be causative in creating an imbalance in normal hematopoiesis.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D006098:Granulocytes / Q000166:cytology*; D007940:Leukemia L5178 / Q000097:blood / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D007958:Leukocyte Count; D008223:Lymphoma / Q000097:blood / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation
D003115:Colony-Stimulating Factors