RNA synthesis inhibitors on young rat adrenal in primary culture. An ultrastructural study.
Adrenal cells of newborn rat adrenals in primary tissue culture were treated with actinomycin D (2, 10 and 25 micrograms/ml), alpha-amanitin (5 and 50 micrograms/ml) and cordycepin (100 micrograms/ml) and studied with the electron microscope. The most striking changes observed with the three drugs were localized on the nucleoli, and consisted, respectively, of segregation (actinomycin D), fragmentation (alpha-amanitin) and microsegregation (cordycepin). These results are consistent with the molecular sites of action of the drugs and are comparable to previous in vivo findings.
D000311:Adrenal Glands / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D003839:Deoxyadenosines / Q000494:pharmacology*; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000187:drug effects
D000546:Amanitins; D003839:Deoxyadenosines; D003609:Dactinomycin; D003345:Corticosterone