Increased sister-chromatid exchange rate and its regression during prolonged incubation in lymphocyte cultures from patients with multiple sclerosis.
Peripheral venous blood lymphocytes from multiple sclerosis patients, cultured for 72 h in the presence of phytohemagglutinin, appeared to have a higher sister-chromatid exchange (SCE) rate than cells from matched controls. Prolongation of the incubation time to 9 days by adding interleukin-2 to the cultures, caused the cells from the MS patients to lose their increased SCE frequency, so that the mean rate no longer differed from that of the controls. The SCE rate of the controls did not change significantly on prolonged incubation.
D000328:Adult; D002453:Cell Cycle; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D009103:Multiple Sclerosis / Q000235:genetics* / Q000473:pathology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013997:Time Factors
D007376:Interleukin-2; D010835:Phytohemagglutinins