Plasmid penicillin resistance in Vibrio cholerae: identification of new beta-lactamase SAR-1.
Two strains of Vibrio cholerae biotype El Tor, isolated in Tanzania, possessed a single IncC resistance plasmid of 113 kilobases. Both plasmids encoded the production of a novel beta-lactamase, SAR-1, which was 33,700 daltons in size and was able to hydrolyze carbenicillin as well as penicillin G. The SAR-1 beta-lactamase was quite distinct from all other plasmid beta-lactamases by virtue of its unusually low isoelectric point and a combination of its size, substrate profile, and inhibition properties. This enzyme is only the second beta-lactamase identified in V. cholerae species and the first to be reported in V. cholerae strains isolated in Southern Africa.
D000667:Ampicillin / Q000494:pharmacology*; D002228:Carbenicillin / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007525:Isoelectric Focusing; D007700:Kinetics; D008970:Molecular Weight; D010400:Penicillin G / Q000378:metabolism; D010403:Penicillin Resistance; D011815:R Factors; D014734:Vibrio cholerae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D000667:Ampicillin; D001618:beta-Lactamases; D002228:Carbenicillin; D010400:Penicillin G