A fluorometric determination of N-methyl-4-phenylpyridinium ion, using high-performance liquid chromatography.
A simple and sensitive assay procedure for the quantitation of N-methyl-4-phenylpyridinium ion (MPP+), a neurotoxin, was devised using its fluorescence and high-performance liquid chromatography (HPLC). The fluorescence intensity of MPP+ was several thousand times more than that of N-methyl-1,2,3,6-tetrahydropyridine (MPTP), a metabolic precursor of MPP+. This method was found to be sensitive enough to measure less than 10 pmol MPP+ without using HPLC and 10 fmol using HPLC. The oxidation of MPTP by monoamine oxidase in human brain synaptosomal mitochondria was examined by this assay method. This fluorometric-HPLC method should have broad application in the study of the neurotoxin MPP+.
D015655:1-Methyl-4-phenylpyridinium; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007735:Kynuramine; D008995:Monoamine Oxidase / Q000378:metabolism; D009498:Neurotoxins / Q000032:analysis*; D010084:Oxidation-Reduction; D011726:Pyridinium Compounds / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013574:Synaptosomes / Q000201:enzymology
D009498:Neurotoxins; D011726:Pyridinium Compounds; D007735:Kynuramine; D008995:Monoamine Oxidase; D015655:1-Methyl-4-phenylpyridinium