title: Reduced gap junctional communication is associated with the lethal condition characteristic of DDK mouse eggs fertilized by foreign sperm.
abstract: Communication through gap junctions was examined in 8-cell zygotes generated by fertilization of eggs of the DDK inbred strain of mice with spermatozoa of the C3H strain. These zygotes spontaneously begin to extrude cells at the late 16-cell stage and 95% die by the blastocyst stage. The transfer of Lucifer Yellow between cells of DDK/C3H zygotes that had not yet begun to express the defect was significantly slower than in DDK/DDK controls or in controls from other strains. Treatment with the weak base methylamine, to raise intracellular pH, speeded the transfer of Lucifer in all strains; transfer between cells of DDK/C3H zygotes became as fast as that between cells of control zygotes. DDK/C3H zygotes cultured in methylamine either from the 4- to 8-cell stage to the early 16-cell stage (19h) or from the early to the late 16-cell stage (6 h) showed significant rescue to the blastocyst stage. Once spontaneous decompaction of cells from DDK/C3H zygotes had begun (the late 16-cell stage onwards) methylamine treatment was no longer able to bring about rescue. We conclude that zygotes developed from eggs of the DDK strain fertilized by foreign spermatozoa are characterized physiologically by defective gap junctional communication. Improving gap junctional communication is sufficient to allow many zygotes to maintain the compacted state, suggesting a link between compaction and communication through gap junctions.
mesh_terms: D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D002678:Chimera; D005314:Embryonic and Fetal Development; D005306:Fertilization; D006863:Hydrogen-Ion Concentration; D007365:Intercellular Junctions / Q000502:physiology*; D007546:Isoquinolines; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D013094:Spermatozoa; D015053:Zygote / Q000502:physiology
chemical_list: D007546:Isoquinolines; C017475:lucifer yellow