title: alpha-Amylase gene of Streptomyces limosus: nucleotide sequence, expression motifs, and amino acid sequence homology to mammalian and invertebrate alpha-amylases.
abstract: The nucleotide sequence of the coding and regulatory regions of the alpha-amylase gene (aml) of Streptomyces limosus was determined. High-resolution S1 mapping was used to locate the 5' end of the transcript and demonstrated that the gene is transcribed from a unique promoter. The predicted amino acid sequence has considerable identity to mammalian and invertebrate alpha-amylases, but not to those of plant, fungal, or eubacterial origin. Consistent with this is the susceptibility of the enzyme to an inhibitor of mammalian alpha-amylases. The amino-terminal sequence of the extracellular enzyme was determined, revealing the presence of a typical signal peptide preceding the mature form of the alpha-amylase.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics*
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D000516:alpha-Amylases