cDNA cloning and chromosomal localization (4q11-13) of a gene for statherin, a regulator of calcium in saliva.
On the basis of the known amino acid sequence of statherin, a human salivary protein, mixed synthetic oligonucleotides were synthesized and used to screen a cDNA library constructed from human parotid-gland mRNA. A cDNA clone coding for statherin was isolated from this library and has been completely sequenced. The cDNA represents a full-length (or nearly full-length) copy of an approximately 640-bp statherin mRNA. Statherin appears to be coded by a single-copy gene that maps to chromosome 4q11-4q13 when somatic-cell hybrids are used.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics; D012471:Salivary Proteins and Peptides / Q000235:genetics*
D009841:Oligonucleotides; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D004247:DNA