The role of interleukin 2 in hemorrhage-induced abnormalities of lymphocyte proliferation.
Depression of lymphocyte proliferative response occurs after trauma and hemorrhage. Because abnormalities in production or utilization of interleukin 2 (IL-2) can result in depression of mitogen-induced lymphocyte proliferation, we investigated the effects of unanesthetized hemorrhage on the generation of IL-2 by rat peripheral blood lymphocytes and the response of these cells to exogenous IL-2. Two hours after loss of 30% of total blood volume, IL-2 production was reduced by greater than 90%. Return to normal levels of IL-2 generation occurred by 48 hr after hemorrhage. Addition of purified rat IL-2 to cultures of phytohemagglutin-stimulated peripheral blood lymphocytes obtained from normal animals resulted in suppression of the proliferative response. Exogenous IL-2 produced a similar degree of suppression in the proliferation of cells obtained from hemorrhaged animals. These results show a profound hemorrhage-induced suppression of IL-2 generation, but no benefits of exogenous IL-2 in improving the depressed lymphocyte proliferative response that exists after hemorrhage.
D000818:Animals; D006470:Hemorrhage / Q000097:blood / Q000276:immunology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D007375:Interleukin-1; D010835:Phytohemagglutinins