Selective induction of OX19+ (CD5+) or OX19- (CD5-) alloreactive cytolytic lymphocytes in the rat.
The phenotypes of alloselective cytolytic lymphocytes of the rat are defined by staining of peritoneal cells of alloimmunized donors with monoclonal antibodies, sorting in a cytofluorometer and evaluating cytolytic capacity in a 51Cr-release assay. We demonstrate that alloimmunization of BN rats can result in either OX19+ (CD5+) or OX19- (CD5-) cytolytic alloselective lymphocytes and show that the OX19- (CD5-) cytolytic cells are OX34+ W3/25- (CD4-) OX8+ (CD8+) lymphocytes not exposing surface Ig. It is further demonstrated that the appearance of CD5+ and CD5- cytolytic alloselective lymphocytes are mutually exclusive; immunization with (WF X BN) F1 cells leading exclusively to appearance of OX19+ effector cells while immunization with WF cells leads to OX19- effector cells. Alloimmunization of WF rats only results in appearance of OX19+ cytolytic lymphocytes.
D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry / Q000379:methods; D007114:Immunization; D010529:Peritoneal Cavity / Q000166:cytology; D010641:Phenotype; D051381:Rats; D011914:Rats, Inbred BN; D011920:Rats, Inbred WF; D011947:Receptors, Antigen, B-Cell / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011947:Receptors, Antigen, B-Cell