Humoral immunity to dietary antigens in atopic dermatitis. II. Analysis of IgE and IgG subclass antibodies.
IgG subclass antibodies to two dietary antigens, ovalbumin (OA) and beta-lactoglobulin (BLG) were measured with quantitative ELISA-techniques in 16 patients with atopic dermatitis (AD) (6-21 years old) and closely matched controls. In addition, IgE-antibodies to OA, BLG and milk were determined with RAST. IgG subclass antibodies were frequently detected in IgG1 and IgG4 for both AD-patients and controls, quantitatively dominated by IgG4. The IgG4 anti-BLG antibody levels were significantly higher (P less than 0.001) in AD-patients (median: 1.1 microgram/ml, range: 0-24.0 microgram/ml) than in controls (median: 0.05 microgram/ml, range: 0-1.05 microgram/ml). No relation was found between IgG4 anti-BLG antibody levels, levels of IgE antibodies to milk or BLG, or severity of disease.
D000293:Adolescent; D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis* / Q000145:classification* / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000008:administration & dosage; D002648:Child; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology*; D004032:Diet; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000145:classification / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D011852:Radioallergosorbent Test
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007074:Immunoglobulin G; D007782:Lactoglobulins; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; D010047:Ovalbumin