title: Regulation of androgen production by frog (Rana esculenta) testis: an in vitro study on the effects exerted by estradiol, 5 alpha-dihydrotestosterone, testosterone, melatonin, and serotonin.
abstract: The possible role of estradiol-17 beta (E2), testosterone (T), 5 alpha-dihydrotestosterone (DHT), melatonin, and serotonin on the regulation of androgen (A) production by the frog, Rana esculenta, testes was studied in vitro. E2 (10(-6) M) inhibited A production whether alone or in combination with oLH (20 micrograms) after 6 hr incubation. After 24 hr incubation. A production was reduced by E2 concentration of around 10(-6) and 10(-9) M. Melatonin and serotonin did not induce any change whichever experimental condition was used. Preincubation for 6 hr with 10(-6) M T or DHT enhanced the oLH-stimulated A production after 18 hr incubation. These data suggest that steroids may regulate their intratesticular levels without passing into the blood stream.
mesh_terms: D000728:Androgens / Q000096:biosynthesis*; D000818:Animals; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D011893:Rana esculenta / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology; D013737:Testis / Q000502:physiology*; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000728:Androgens; D013196:Dihydrotestosterone; D012701:Serotonin; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D008550:Melatonin