Mutation at the APRT locus in Friend erythroleukaemia cells. 1. Mutation rates and properties of mutants.
Spontaneous mutation at the adenine phosphoribosyl transferase (APRT) locus in clone 707 of the Friend cell line was examined. The frequency of cells resistant to 2,6-diaminopurine (DAP) was found to be 2.6 X 10(-5) with a mutation rate of 1.81 X 10(-6) cell-1 generation-1. APRT activities in 9 DAP-resistant clones were found to vary between 0 and 27% the level observed in wild-type cells. It is suggested that clone 707 cells are heterozygous or functionally hemizygous at the APRT locus.
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology; D007942:Leukemia, Experimental / Q000201:enzymology*; D051379:Mice; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*
D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase