Effect of alpha-1-antichymotrypsin on DNA synthesis in permeabilized human cells.
The effect of Alpha-1-antichymotrypsin(ACT) on DNA synthesis was studied using lysolecithin-permeabilized cultured human stomach carcinoma cells. ACT added in medium inhibited DNA synthesis and the degree of inhibition is incubation time dependent. It is proportional to ACT concentration and the concentration of ACT required for 50% inhibition was 0.8 mg/ml.
D000230:Adenocarcinoma / Q000378:metabolism; D002460:Cell Line; D002463:Cell Membrane Permeability; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D006801:Humans; D008244:Lysophosphatidylcholines; D013274:Stomach Neoplasms / Q000378:metabolism; D000514:alpha 1-Antichymotrypsin / Q000378:metabolism / Q000494:pharmacology*
D004273:DNA, Neoplasm; D008244:Lysophosphatidylcholines; D000514:alpha 1-Antichymotrypsin; D004257:DNA Polymerase II