Modulation of Friend virus infectivity in vivo by administration of purified preparations of human lactoferrin and recombinant murine interleukin-3 to mice.
Purified iron-saturated human milk lactoferrin (LF) and purified recombinant murine interleukin-3 (IL-3) were assessed in vivo for their effects on replication of spleen focus forming viruses (SFFV) in spleens of DBA/2 mice injected with the polycythemia-inducing strain of the Friend virus complex. LF and IL-3, inoculated 2 hr prior to the administration of the polycythemia-inducing strain of the Friend virus complex, respectively decreased and increased the replication of SFFV in mice as assessed by the spleen focus forming unit assay in primary and secondary DBA/2 mice. Since virus infectivity is associated with the DNA synthetic phase of the cell cycle and it has been shown elsewhere that LF decreases and IL-3 increases the percent of hematopoietic progenitor cells in S-phase in vivo, the results suggest that the opposing actions of LF and IL-3 on replication of SFFV may reflect the actions of these molecules on cycling of the target cells for SFFV.
D000818:Animals; D005260:Female; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000472:pathogenicity*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D007781:Lactoferrin / Q000494:pharmacology*; D007782:Lactoglobulins / Q000494:pharmacology*; D051379:Mice; D008811:Mice, Inbred DBA; D011086:Polycythemia / Q000382:microbiology; D011994:Recombinant Proteins / Q000494:pharmacology*; D013155:Spleen Focus-Forming Viruses / Q000187:drug effects / Q000472:pathogenicity; D014779:Virus Replication / Q000187:drug effects
D007377:Interleukin-3; D007782:Lactoglobulins; D011994:Recombinant Proteins; D007781:Lactoferrin