Detection of HTLV-III antibody in cerebrospinal fluid from patients with AIDS and pre-AIDS with the use of a commercial test system.
Sixty-one paired sera and cerebrospinal fluid (CSF) specimens were tested for the presence of human T-cell lymphotropic virus/lymphadenopathy-associated virus (HTLV-III) antibodies. Of 16 sera negative for HTLV-III antibodies by enzyme-linked immunosorbent assay (ELISA), the corresponding CSF results were also negative. Forty-two of 45 (93%) CSF specimens tested (in which the corresponding sera were HTLV-III antibody positive) were positive by ELISA. Western blot analysis (WB) of 24 paired specimens, as well as retrospectively acquired case histories, confirmed the results obtained by ELISA.
D000386:AIDS-Related Complex / Q000134:cerebrospinal fluid* / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000134:cerebrospinal fluid* / Q000276:immunology; D000914:Antibodies, Viral / Q000134:cerebrospinal fluid*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans; D007122:Immunoelectrophoresis; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic