Cloning of early erythroid and mixed myeloid/erythroid human bone marrow progenitor cells: comparison of different sources of burst-promoting activity (BPA).
The ability of conditioned media from the 5637 cell line and human placenta (HPCM) to stimulate the in vitro growth of human early erythroid and mixed myeloid/erythroid clones was tested and compared to phytohemagglutinin-stimulated leukocyte supernatants (PHA-LCM). Both 5637 supernatant and PHA-LCM were equally effective with a linear dose-response relationship. HPCM at various concentrations did not exhibit burst-promoting activity (BPA). Thus, "pluripoietin"-containing media provide a large-scale source of BPA similar in its biological activity to standard sources used for studying human hematopoiesis in vitro.
D002999:Clone Cells / Q000166:cytology / Q000502:physiology; D003115:Colony-Stimulating Factors / Q000502:physiology*; D004920:Erythropoiesis; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D010835:Phytohemagglutinins / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D010835:Phytohemagglutinins