title: Natural killer cell activity in the rat. V. The circulation patterns and tissue localization of peripheral blood large granular lymphocytes (LGL).
abstract: Large granular lymphocytes (LGL) and T cells were separated from blood by centrifugation on discontinuous gradients of Percoll, were labeled with [3H]uridine or [111In]oxine, and were injected i.v. into syngeneic euthymic or athymic nude rats. The tissue distribution of these labeled cells was monitored for up to 24 hr after transfer by scintillation counting of tissue homogenates and autoradiography of tissue sections. In normal euthymic rats, the main sites of LGL localization were the alveolar walls of the lungs and spleen red pulp; however, they were not detectable in the major traffic areas of T lymphocyte recirculation, the spleen white pulp, and lymph nodes. Furthermore, the density of labeled LGL was very low in the small intestine, thymus, kidney, and liver, although on a per-organ basis, about 10% of the injected radioactivity was found in the liver by 24 hr post-injection. When 111In-labeled LGL were injected i.v. into rats with an indwelling thoracic duct cannula, they completely failed to enter the thoracic duct lymphocyte (TDL) population over an observation period of 6 days. This finding was markedly different from the results obtained with T cells and was consistent with the lack of natural killer and antibody-dependent cellular cytotoxicity activity observed among TDL, even in rats pretreated with the biological response modifier, poly I:C. LGL in athymic nude rats also failed to recirculate between blood and lymph. However, in contrast to normal euthymic animals, a significant increase in the localization of radiolabeled LGL to lymph nodes was observed in nude rats between 30 min and 24 hr. Taken as a whole, these findings define the areas within the lungs and spleen in which blood LGL normally localize, and clearly demonstrate that LGL do not normally recirculate between blood and lymph.
mesh_terms: D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001345:Autoradiography; D001775:Blood Circulation; D002465:Cell Movement; D007275:Injections, Intravenous; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology* / Q000637:transplantation; D008196:Lymph / Q000166:cytology / Q000502:physiology; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology* / Q000502:physiology; D008297:Male; D009928:Organ Specificity; D011650:Pulmonary Alveoli / Q000166:cytology; D051381:Rats; D011916:Rats, Inbred F344; D011922:Rats, Mutant Strains; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000502:physiology / Q000637:transplantation; D013897:Thoracic Duct / Q000166:cytology; D014529:Uridine / Q000378:metabolism
chemical_list: D014529:Uridine