Androgen regulated expression of a spermine binding protein gene in mouse ventral prostate.
A full length cDNA (MP25) encoding the major mouse prostatic secretory glycoprotein (p25), whose expression is androgen dependent, has been cloned and characterised. Steady-state levels of mRNA are decreased approximately 100-fold after 3 days castration but are restored progressively over 4 days with testosterone treatment. The secreted glycoprotein appears to be a spermine binding protein since the nucleotide and predicted amino acid sequence of MP25 shares extensive homology with a spermine binding protein (SBP) found in rat ventral prostate. Genomic clones indicate that there is a single gene for SBP which consists of 4 exons, the first of which is only 11bp in length. The second exon encodes the signal peptide, the third contains a portion of the spermine binding protein unique to the mouse and the largest exon encodes the bulk of the secreted protein.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics*; D010088:Oxidoreductases; D011467:Prostate / Q000378:metabolism*; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013096:Spermine / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology*
D002352:Carrier Proteins; D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger; C032603:Sbp protein, rat; D013096:Spermine; D013739:Testosterone; D004247:DNA; D010088:Oxidoreductases; C053986:Sbp protein, mouse