In vivo voltammetric characterization of low affinity striatal dopamine uptake: drug inhibition profile and relation to dopaminergic innervation density.
Electrical stimulation of the median forebrain bundle evoked dopamine release in the ipsilateral striatum which was monitored with high-speed cyclic voltammetry. After stimulation, the extracellular concentration of dopamine fell due to uptake in a biphasic manner, showing zero-order and first-order components. The zero-order phase corresponded to a Vmax of 42.8 +/- 1.8 nmol/min/g tissue. The uptake system could be blocked by D-amphetamine, methylphenidate and nomifensine, but not by benztropine, amfonelic acid or mazindol. The density of uptake sites showed a correlation with the density of striatal dopamine innervation.
D000818:Animals; D001590:Benztropine / Q000494:pharmacology; D003342:Corpus Striatum / Q000378:metabolism*; D003913:Dextroamphetamine / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D004558:Electric Stimulation; D008297:Male; D008454:Mazindol / Q000494:pharmacology; D008474:Medial Forebrain Bundle / Q000502:physiology*; D008774:Methylphenidate / Q000494:pharmacology; D009268:Nalidixic Acid / Q000031:analogs & derivatives; D009287:Naphthyridines / Q000494:pharmacology; D009434:Neural Pathways / Q000502:physiology*; D009627:Nomifensine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009287:Naphthyridines; D009627:Nomifensine; D001590:Benztropine; D008774:Methylphenidate; D009268:Nalidixic Acid; D008454:Mazindol; C006486:amfonelic acid; D003913:Dextroamphetamine; D004298:Dopamine