Bioluminescent assay of D-3-hydroxybutyrate in serum.
An enzymatic assay of D-3-hydroxybutyrate in which the hydroxybutyrate dehydrogenase reaction is coupled to the bacterial oxidoreductase-luciferase system is described. The bioluminescent assay is based on either, end-point, or on initial velocity measurements. This simple and rapid assay requires a single serum sample of 10 microliters. Its linear range covers two orders of magnitude from 10(-6) mol/l upwards. This assay is suitable for the routine determination of D-3-hydroxybutyrate in human blood with good accuracy.
D020155:3-Hydroxybutyric Acid; D006801:Humans; D006884:Hydroxybutyrate Dehydrogenase; D006885:Hydroxybutyrates / Q000097:blood*; D008156:Luciferases; D008163:Luminescent Measurements; D009243:NAD
D006885:Hydroxybutyrates; D009243:NAD; D006884:Hydroxybutyrate Dehydrogenase; D008156:Luciferases; D020155:3-Hydroxybutyric Acid