P-450(11beta)-dependent conversion of androgen to estrogen, the aromatase reaction.
Purified bovine adrenal P-450(11)beta has been shown to act as an aromatase which catalyzes conversion of 19-oxoandrostenedione to estrone. No conversions took place when any one of the required components such as NADPH, NADPH:adrenodoxin reductase, adrenodoxin and P-450(11)beta was omitted from the complete reconstituted system. P-450scc, another mitochondrial P-450 obtained from adrenal cortex, did not substitute for the P-450(11)beta in the aromatase reaction. These results show that P-450(11)beta is able to catalyze a series of reaction which can generate adrenal estrogen through androstenedione and its 19-hydroxy- and 19-oxo-derivatives. The P-450(11)beta-dependent reaction appears to be quite different from the placental aromatase reaction in that the latter is catalyzed by a microsomal P-450.
D000728:Androgens / Q000378:metabolism*; D000735:Androstenedione / Q000031:analogs & derivatives / Q000378:metabolism; D001141:Aromatase / Q000378:metabolism*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004967:Estrogens / Q000096:biosynthesis*; D004970:Estrone / Q000096:biosynthesis; D008401:Gas Chromatography-Mass Spectrometry; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*
D000728:Androgens; D004967:Estrogens; D004970:Estrone; D000735:Androstenedione; D003577:Cytochrome P-450 Enzyme System; C014142:19-oxo-delta(4) androstene-3,17-dione; D013250:Steroid Hydroxylases; D001141:Aromatase; D013252:Steroid 11-beta-Hydroxylase