Retinoblastoma. Immunohistochemistry and cell differentiation.
Tumor from eight enucleated eyes was analyzed by immunohistochemistry, using a panel of specific antibodies including interphotoreceptor retinoid-binding protein (IRBP), S-antigen (S-Ag), opsin, neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP), laminin, and vimentin. In addition, immunoelectron microscopy and enzyme-linked immunosorbent assay (ELISA) for IRBP were performed. Immunohistochemical staining disclosed the most pronounced labeling of tumor cells with NSE and IRBP antibodies. A correlation was found between the degree of tumor differentiation and amount of IRBP, a protein specifically synthesized by photoreceptor cells. Moderate labeling of the better differentiated tumors was also observed with antibodies against S-Ag and focal labeling in a few tumors with opsin antibodies. Anti-GFAP labeling was limited to a smaller number of reactive glial cells and perivascular glial cells. These data indicate the essential neuronal nature of retinoblastoma tumor cells in situ as well as at least partial photoreceptor-like features, as shown by the presence of recognized photoreceptor cell markers (IRBP, S-Ag, opsin). Tissue culture studies using the human Y-79 retinoblastoma cell line also demonstrate that the tumor cells are primitive multipotential retinoblasts capable of at least partial differentiation along neuronal, glial, or pigment epithelial cell lines.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D002454:Cell Differentiation; D002675:Child, Preschool; D005134:Eye Neoplasms / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D005136:Eye Proteins / Q000276:immunology; D006801:Humans; D007223:Infant; D012175:Retinoblastoma / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D012177:Retinol-Binding Proteins / Q000032:analysis / Q000276:immunology; D000099214:Retinol-Binding Proteins, Interstitial
D000888:Antibodies, Anti-Idiotypic; D005136:Eye Proteins; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial