Immunosuppression by canine distemper virus: modulation of in vitro immunoglobulin synthesis, interleukin release and prostaglandin E2 production.
In vitro or in vivo infection of canine mononuclear cells by canine distemper virus (CDV) in short-term microcultures resulted in suppression of lectin-induced 3H-thymidine incorporation. This suppressive effect was also evident in pokeweed mitogen-driven in vitro immunoglobulin synthesis and release. Lectin-induced interleukin-2 production by monocyte-depleted lymphocyte cultures was marginally affected by CDV, whereas interleukin-1 production by adherent mononuclear cells was significantly depressed. Monocyte cultures established from viremic dogs released prostaglandin (PG)E2. The results suggest that, in addition to a direct viral effect upon lectin responsive cellular population(s), CDV modulates monocyte functions by inhibition of interleukin-1 production and by enhancing PGE2 release.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D015232:Dinoprostone; D004216:Distemper / Q000276:immunology*; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D007136:Immunoglobulins / Q000096:biosynthesis*; D007165:Immunosuppression Therapy; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D011458:Prostaglandins E / Q000096:biosynthesis*
D007136:Immunoglobulins; D007375:Interleukin-1; D007376:Interleukin-2; D011458:Prostaglandins E; D015232:Dinoprostone; D007213:Indomethacin