Ultrastructural and phenotypic changes in Langerhans cells induced in vitro by contact allergens.
Ultrastructural changes of murine Langerhans cells (LC) were examined following exposure of crude epidermal cell suspensions to the contact allergens dinitrochlorobenzene, nickel sulphate and lead nitrate at various concentrations and for various incubation times. An immunogold labelling technique was employed to study changes in surface expression of MHC Class II (Ia) molecules. In all cases, activation of LC was evident after as little as 15 min exposure and was characterized by a marked increase in surface expression of Ia molecules, prominent rough endoplasmic reticulum and numerous ribosomes and lysosomes. Degenerative changes in LC were apparent to varying degrees depending on the allergen, its concentration and the time of incubation. Degenerative changes included swollen mitochondria, membrane disruption or rupture, loss of density of the cytoplasm (cytolysis), loss of dendritic processes and decreased expression of Ia molecules. In the case of dinitrochlorobenzene, degenerative changes were present and usually severe at concentrations greater than 10 micrograms/ml, while exposure to nickel sulphate and lead nitrate was associated with only mild degenerative changes. These observations indicate that contact allergens have a variety of direct effects on LC, including activation and degeneration, which are dose- and time-dependent. Since these alterations of LC were observed in the absence of other immunologically active cells, peripolesis cannot be involved in these events.
D000485:Allergens / Q000276:immunology*; D000818:Animals; D002478:Cells, Cultured; D004137:Dinitrochlorobenzene / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004342:Drug Hypersensitivity / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006968:Hypersensitivity, Delayed / Q000276:immunology; D066298:In Vitro Techniques; D007801:Langerhans Cells / Q000276:immunology / Q000648:ultrastructure*; D007854:Lead / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009532:Nickel / Q000276:immunology*; D009566:Nitrates / Q000276:immunology*
D000485:Allergens; D004137:Dinitrochlorobenzene; D000949:Histocompatibility Antigens Class II; D009566:Nitrates; D007854:Lead; C029938:nickel sulfate; C017461:lead nitrate; D009532:Nickel