title: Individual variation in immunosuppressive activity of bovine seminal plasma on concanavalin A-stimulated bovine T lymphocytes in vitro.
abstract: Individual dialyzed seminal plasma samples from 21 bulls in routine artificial breeding service were tested for their immunosuppressive activity on in vitro induced blastogenesis of bovine lymphocytes from 3 nonpregnant cows. Concanavalin A was used to induce blastogenesis and thymidine uptake was monitored. Dialyzed seminal plasma from 15 of 21 bulls inhibited deoxyribonucleic acid synthesis response to concanavalin with cow A lymphocytes, and 21 of 21 were immunosuppressive to lymphocytes from cows B and C. Degree of immunosuppression varied according to bull and cow, ranging from 0 to 100%. The lower the percentage response of the lymphocytes to concanavalin stimulation, the less their inhibition by dialyzed seminal plasma. The degree of immunosuppression produced by a bull's dialyzed seminal plasma was not highly related either to amount of protein in the seminal plasma or to the bull's 60 to 90-d nonreturn rate.
mesh_terms: D000818:Animals; D002417:Cattle; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D014644:Genetic Variation; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D007315:Insemination, Artificial / Q000662:veterinary; D008213:Lymphocyte Activation; D008297:Male; D012661:Semen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D003208:Concanavalin A