Metronidazole-resistant clinical isolates of Trichomonas vaginalis have lowered oxygen affinities.
Oxygen affinities of metronidazole susceptible and resistant isolates of the parasitic flagellate protozoon Trichomonas vaginalis were determined by mass spectrometric methods. Apparent O2Km values for the respiration of non-proliferating cell suspensions were about 10-fold higher for metronidazole resistant strains than for the susceptible strains C1-NIH or NYH-286. Simultaneous monitoring of hydrogen evolution in the presence of increasing O2 tensions enabled apparent Ki values for H2 to be determined; and this function was independent of metronidazole susceptibility. Apparent O2 affinities of the hydrogenosomal and non-sedimentable fractions were determined for the strains CDC 85 (metronidazole resistant) and C1-NIH, which showed the deficiency in the O2 scavenging capacity by the resistant strain to be associated with the hydrogenosome-containing fraction.
D000818:Animals; D004351:Drug Resistance; D007700:Kinetics; D008795:Metronidazole / Q000494:pharmacology*; D010101:Oxygen Consumption; D014246:Trichomonas vaginalis / Q000187:drug effects / Q000378:metabolism*
D008795:Metronidazole