Radiation-induced lipid peroxidation: influence of oxygen concentration and membrane lipid composition.
Radiation-induced lipid peroxidation in phospholipid liposomes was investigated in terms of its dependence on lipid composition and oxygen concentration. Non-peroxidizable lipid incorporated in the liposomes reduced the rate of peroxidation of the peroxidizable phospholipid acyl chains, possibly by restricting the length of chain reactions. The latter effect is believed to be caused by interference of the non-peroxidizable lipids in the bilayer. At low oxygen concentration lipid peroxidation was reduced. The cause of this limited peroxidation may be a reduced number of radical initiation reactions possibly involving oxygen-derived superoxide radicals. Killing of proliferating mammalian cells, irradiated at oxygen concentrations ranging from 0 to 100 per cent, appeared to be independent of the concentration of peroxidizable phospholipids in the cell membranes. This indicates that lipid peroxidation is not the determining process in radiation-induced reproductive cell death.
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured / Q000528:radiation effects; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D008081:Liposomes / Q000528:radiation effects*; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D010100:Oxygen / Q000378:metabolism; D013329:Structure-Activity Relationship; D014965:X-Rays
D005231:Fatty Acids, Unsaturated; D008054:Lipid Peroxides; D008081:Liposomes; D008563:Membrane Lipids; D010100:Oxygen