Molecular definition of retrovirus-induced antigens recognized by tumour-specific H-2-restricted cytolytic T lymphocytes.
The antigenic specificities of H-2-restricted, tumour-specific cytolytic T lymphocytes (CTL) were studied at the molecular level using CTL from BALB.B and C57BL/6 (H-2b) mice sensitized to an H-2b Gross murine leukaemia virus (MuLV)-induced tumour. Target cells were produced by the double transfection of mouse L cells (H-2k) with the cloned H-2Kb or H-2Db gene and retroviral DNA derived from a molecular clone of Akv MuLV (closely related to Gross MuLV). Doubly transfected L cells which express either H-2Kb or H-2Db antigen and retroviral antigens are lysed in a virus-specific manner by Gross MuLV-immune CTL. The existence of two independent Gross MuLV-immune CTL subpopulations, one restricted by H-2Kb and the other by H-2Db, is thus confirmed. Gross MuLV-immune CTL from both BALB.B and C57BL/6 mice killed L cells that express Akv MuLV gag gene products and H-2Kb or H-2Db antigen. In contrast, only CTL from C57BL/6 mice killed L cells that express Akv MuLV env gene products and H-2Kb or H-2Db. This indicates that specific recognition of MuLV-induced antigens by CTL can be selective and varies according to the origin of the CTL.
D000406:AKR murine leukemia virus / Q000235:genetics / Q000276:immunology; D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral / Q000235:genetics; D002460:Cell Line; D005814:Genes, Viral; D006183:H-2 Antigens / Q000276:immunology; D007739:L Cells / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014412:Tumor Virus Infections / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D006183:H-2 Antigens