Interference with Fc signals increases an antibody response by T-cell-deprived cultures to a T-dependent antigen.
Affinity column purified goat anti-mouse immunoglobulin antibodies specific for the Fc portion of IgG increased an in vitro antibody response to a T-dependent antigen when T cells were limiting. Picogram amounts of specific anti-Fc antibody at culture initiation and nanogram quantities up to 3 days were required to demonstrate this effect. The demonstration of reconstitution by anti-Fc antibodies requires that the cultures be T-cell depleted and stimulated by antigen. These results support the concept that anti-Fc antibody and T cells block endogenously generated negative Fc signals.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D007108:Immune Tolerance; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007141:Immunoglobulin Fc Fragments