title: An in vitro model for the study of acute release of von Willebrand factor from human endothelial cells.
abstract: An in vitro model is described which utilizes human umbilical vein endothelial cells cultured on plastic microcarrier spheres and perfused with serum-free medium. This model was used to study the acute release of von Willebrand factor following stimulation of the cells with putative agonists. Thrombin, plasmin and interleukin-1 were found to release von Willebrand factor. Adrenaline and bradykinin also stimulated release but only at high dosage. 1-desamino-8-D-arginine vasopressin (DDAVP) was inactive.
mesh_terms: D001920:Bradykinin / Q000494:pharmacology; D002478:Cells, Cultured; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology; D005343:Fibrinolytic Agents / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008954:Models, Biological; D013917:Thrombin / Q000494:pharmacology; D014471:Umbilical Veins / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D005343:Fibrinolytic Agents; D007375:Interleukin-1; D014841:von Willebrand Factor; D013917:Thrombin; D001920:Bradykinin; D004837:Epinephrine