Transformation induced by Echinococcus granulosus protoscoleces in unprimed murine spleen cells: identity and MHC restriction of participating cell types.
Transformation induced by Echinococcus granulosus in unprimed murine spleen cells in vitro was not prevented by rigorous T- or B-cell depletion. When naïve T- or B-cell populations were further depleted of macrophages, B-cell transformation was relatively unaffected whereas T-cell transformation was totally inhibited. Peritoneal macrophages from infected mice could present a mitogenic stimulus to purified, naïve T-cells, provided that the T-cells had identity in the I region of the H2 complex. K/D identity was neither necessary nor sufficient. It is concluded that transformation can be induced by E. granulosus in both T- and B-cells and that, at least in naïve T-cells, the stimulus can be an I region-restricted, macrophage-presented signal.
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D004446:Echinococcus / Q000276:immunology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000947:Antigens, Helminth