Occurrence of two beta-tubulin isoforms with different polymerizing abilities in L5178Y cells.
Mouse lymphoma L5178Y cells express at least two isoforms of beta-tubulin, designated M beta I and M beta II, as revealed by isoelectrofocusing, whereas two independently isolated normal T-cell clones, 3D10 and K23, express only M beta I. M beta II-tubulin is more acidic (pI, 5.10) than M beta I-tubulin (pI, 5.15). L5178Y cells were disrupted under the microtubule-stabilizing conditions, followed by centrifugation to separate fractions containing polymerized and unpolymerized tubulin. We found that a proportion of M beta II to total beta-tubulins is larger in the fraction containing unpolymerized tubulin than in that containing polymerized tubulin. In addition, when tubulin was purified from extracts of L5178Y cells by repeated cycles of polymerization-depolymerization, the M beta II-tubulin isoform was gradually lost during the successive purification steps. The low recovery of M beta II-tubulin was observed, irrespective of the presence or absence of MAPs, and even in the presence of an excess amount of essentially polymerizable porcine brain tubulin. These results indicate that M beta II-tubulin is less able to polymerize than is M beta I-tubulin, both in vivo and in vitro.
D000818:Animals; D002460:Cell Line; D008214:Lymphocytes / Q000032:analysis / Q000648:ultrastructure; D008223:Lymphoma / Q000032:analysis; D051379:Mice; D008870:Microtubules / Q000648:ultrastructure; D011108:Polymers; D013601:T-Lymphocytes / Q000032:analysis; D014404:Tubulin / Q000032:analysis* / Q000378:metabolism
D011108:Polymers; D014404:Tubulin