The association of the rabies glycoprotein with liposome (immunosome) induces an in vitro specific release of interleukin 2.
BALB/c mice were primed by receiving a unique intraperitoneal injection of rabies virus antigens presented as complete inactivated virus (P.V. strain) or as purified glycoproteins either in the aggregated form or in physical combination with liposomes (i.e., in the form of "immunosomes"). The splenocytes of these mice were restimulated, 6-15 days after priming, in culture with rabies virus antigens, and antigen-specific IL-2 production was measured. It was found that rabies antigens presented as immunosomes were as active as the inactivated virus, whereas equivalent amounts of purified glycoproteins were inactive. The optimal amounts of rabies immunosomes used for priming was found to be 0.5 to 0.05 micrograms per mouse.
D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage / Q000276:immunology*; D002478:Cells, Cultured; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D007114:Immunization / Q000379:methods*; D007376:Interleukin-2 / Q000378:metabolism*; D008081:Liposomes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology*
D000956:Antigens, Viral; D006023:Glycoproteins; D007376:Interleukin-2; D008081:Liposomes; D014764:Viral Proteins