title: 31P-NMR spectra of the Ha-ras p21.nucleotide complexes.
abstract: Phosphorus nuclear magnetic resonance spectra of the Ha-ras oncogene product p21 and its nucleotide complexes have been obtained. It is shown that the 31P nuclear magnetic resonance spectra of a number of nucleotide-enzyme complexes show some common features. In particular, the chemical shift values of the beta-phosphorus resonance of enzyme-bound NTP and NDP (N = A, G) of hydrolases exhibit a downfield shift virtually identical for myosin, elongation factor Tu, and the Ha-ras oncogene product p21. This suggests that the stereochemistry around the beta-phosphorus might be similar in these compounds.
mesh_terms: D006153:Guanosine Diphosphate / Q000032:analysis; D006160:Guanosine Triphosphate / Q000032:analysis; D008274:Magnesium / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D009363:Neoplasm Proteins / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D015689:Oncogene Protein p21(ras); D009857:Oncogenes; D010758:Phosphorus; D011485:Protein Binding
chemical_list: D009363:Neoplasm Proteins; D009711:Nucleotides; D006153:Guanosine Diphosphate; D010758:Phosphorus; D006160:Guanosine Triphosphate; D015689:Oncogene Protein p21(ras); D008274:Magnesium