Screening tests for Sezary cells in cutaneous T-cell lymphomas.
Blood samples from normal individuals (n = 7), from patients with cutaneous T-cell lymphomas (Sezary syndrome = 7; mycosis fungoides = 18) and from patients with chronic benign skin disease (n = 8) were examined for the presence of Sezary cells. The samples were analysed using a Coulter model S Plus IV with a three Cell Population upgrade, and a Becton Dickinson FACS analyser; and the results were compared with those obtained from morphological examination of peripheral blood films. Using the FACS analyser a population of aneuploid cells was only identified in two out of eight patients with confirmed circulating Sezary cells. This was in contrast to the results from the Coulter S Plus IV which detected an abnormality in six out of the eight samples with confirmed circulating Sezary cells. These results indicate that leukocyte volume analysis is a useful, additional screening tool for the identification of Sezary cells.
D005434:Flow Cytometry; D006801:Humans; D007962:Leukocytes / Q000473:pathology; D008223:Lymphoma / Q000473:pathology*; D009182:Mycosis Fungoides / Q000473:pathology; D012751:Sezary Syndrome / Q000473:pathology*; D012871:Skin Diseases / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology*