Monkey B-lymphotropic papovavirus genome: the entire DNA sequence and variable regions.
DNA from monkey B-lymphotropic papovavirus (LPV) adapted to growth in human B-lymphoblastoid cell line BJA-B consisted of three classes of molecules of 5.1, 5.0, and 4.9 kilobases (kb), and the entire DNA sequence of 5.1-kb molecule was determined. The three types of LPV DNA yielded infectious virus upon transfection to BJA-B cells. Comparison of the structures among these clones and clone K38 sequenced by Pawlita et al. (1985) suggests that the transcriptional control region, the small-T antigen gene, and the VP-1 gene are variable in the LPV genome, and that the 5.0-kb LPV resembles the parent virus, from which all the others had evolved by duplication and deletion during passages in BJA-B cells, probably for adaptation to better growth in human cells.
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D001402:B-Lymphocytes / Q000382:microbiology; D001483:Base Sequence; D002522:Chlorocebus aethiops; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes; D005809:Genes, Regulator; D027383:Papillomaviridae / Q000235:genetics*; D027382:Polyomaviridae; D014158:Transcription, Genetic
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral