[Detection of intracytoplasmic immunoglobulins by cytofluorography].
In this report the authors describe a method of cell permeabilisation compatible with flow cytometry. To maintain cell integrity, lysolecithin is used. This method is simple, rapid, and reproducible. Control tests demonstrated no non-specific fluorescence due to lysolecithin treatment. This technique was used in several cases of chronic lymphocytic leukemia to detect intracytoplasmic immunoglobulins and monoclonality and in one case of multiple myeloma.
D001402:B-Lymphocytes / Q000276:immunology; D002463:Cell Membrane Permeability / Q000187:drug effects; D003593:Cytoplasm / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D009101:Multiple Myeloma / Q000276:immunology*
D007136:Immunoglobulins; D008244:Lysophosphatidylcholines
Détection d'immunoglobulines intracytoplasmiques sur cytofluorographe.