title: Differentiation-associated carbohydrate chain on human hematopoietic cells recognized by Clerodendron trichotomum lectin.
abstract: In the screening of hematopoietic cell line cell aggregations, the extract of Clerodendron trichotomum seed was found to aggregate K-562 and KG-1 specifically. In the flow cytometric analysis using FITC-conjugated purified CTL, it was confirmed that CTL recognizes the specific carbohydrate(s) which seem to appear only in the early stages of differentiation of myeloid (KG-1) and erythroid (K-562) cell line cells and erythrocytes. The CTL binding to K-562 cells was decreased by TPA treatment which is known to induce retrodifferentiation of K-562. It is also found that this carbohydrate(s) were shaded with NANA on the differentiated cells. In the erythrocyte, CTL receptor was partially shaded by NANA.
mesh_terms: D050260:Carbohydrate Metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: C051213:Clerodendron trichotomum lectin; D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; D013755:Tetradecanoylphorbol Acetate