title: A system for the partial purification of the human androgen receptor following reversible denaturation.
abstract: Androgen receptors from normal human foreskins were partially purified by sequential phosphocellulose chromatography and affinity chromatography resulting in a 28,000-fold purification and an 81% recovery. SDS-electrophoresis of the partially purified receptor preparation demonstrated that binding activity could be recovered and showed two peaks of specific binding mol. wt 35,000-55,000 and 85,000-105,000). This method demonstrates that androgen receptors can withstand harsh denaturation conditions and should prove to be a valuable tool for purifying the human androgen receptor.
mesh_terms: D002845:Chromatography; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004963:Estrenes / Q000378:metabolism; D006801:Humans; D008297:Male; D015741:Metribolone; D010413:Penis; D011489:Protein Denaturation; D011944:Receptors, Androgen / Q000302:isolation & purification* / Q000378:metabolism; D012867:Skin / Q000032:analysis
chemical_list: D004963:Estrenes; D011944:Receptors, Androgen; D015741:Metribolone