[Studies on the RAST method using tray added disc].
The radioallergosorbent test (RAST) provides a reliable method for detecting of specific IgE antibodies against a wide variety of antigens. The method using tube added disc for the estimation have been widely used in routine assay. In this study, the method using tray added disc has developed in our laboratory. The results were as follows; (1) The method using tray showed good correlation to the original method. (2) Characteristics of the method are rapidity, simplicity and economics. (3) The reaction well of tray is flat bottomed and is good for disc reaction. (4) Coefficients of variation for intra assay were; (d1): 9.40-9.68%, inter assay were 10.39-12.15% respectively. (5) The seasonal variation of positive allergens and the correlation of d to h, g1 to g3 were estimated. The method using tray seems to be one of the most useful routine methods of RAST assay and checking the seasonal variation of RAST will give good advantages to estimate the regional pollution of pollens allergy.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D011852:Radioallergosorbent Test / Q000295:instrumentation / Q000379:methods*; D011863:Radioimmunoassay / Q000379:methods*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; C026408:anti-IgE antibodies; D007073:Immunoglobulin E