High-performance liquid chromatographic analysis of vancomycin in plasma, bone, atrial appendage tissue and pericardial fluid.
Solid-phase extraction coupled with reversed-phase high-performance liquid chromatography and UV detection was employed for the analysis of the antibiotic vancomycin in patient plasma, bone, atrial appendage, and pericardial fluid. Vancomycin was quantitated in samples from patients undergoing cardiac surgery. Calibrations were linear in the range 3-100 micrograms/ml vancomycin; the lower limit of detection was approximately 3 micrograms/ml in fluids with an absolute limit of detection in bone samples of 0.75 microgram per injection.
D001842:Bone and Bones / Q000032:analysis*; D002437:Cefazolin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D001026:Coronary Artery Bypass; D006801:Humans; D009206:Myocardium / Q000032:analysis*; D010490:Pericardial Effusion / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014640:Vancomycin / Q000032:analysis* / Q000097:blood
D014640:Vancomycin; D002437:Cefazolin