title: Chronic exercise stress in mice depresses splenic T lymphocyte mitogenesis in vitro.
abstract: This study investigated changes in functional response to splenic T lymphocytes of mitogens following acute and chronic exposure to endurance exercise. Splenic T cell response in vitro to concanavalin A (Con A) and the total number of lymphocytes per spleen were compared between mice assigned to the following treatment conditions: (a) exercise training (EX) by treadmill running (28 m/min, 8 degrees slope for 30 min, 5 times per week for 4 weeks preceded by 2 weeks of endurance build-up), (b) exercise training as above followed by a single, acute bout of exercise to exhaustion (EX + AC) (35 m/min, 8 degrees slope, 30 min to 2 h duration) (c) exposure to the novel environment for 6 weeks without exercise (control), and exposure to the novel environment as in (c) followed by a single, acute bout of exercise to exhaustion. Treadmill running for 6 weeks significantly enhanced succinate dehydrogenase activity in skeletal muscle compared to the sedentary, control condition, and was broadly interpreted as indicative of a training effect. EX mice had significantly reduced splenic lymphocyte proliferative responses to optimal and supraoptimal concentrations of Con A compared with control animals. Incorporation of [3H]thymidine into splenic lymphocytes from EX + AC mice was the most markedly depressed. Total number of lymphocytes per spleen was significantly lower in EX compared with control mice. These results suggests that chronic exercise challenge in mice is associated with T lymphocyte hyporesponsiveness in the secondary lymphoid organs, such as the spleen.
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000201:enzymology; D005082:Physical Exertion; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D003208:Concanavalin A; D013385:Succinate Dehydrogenase; D013936:Thymidine