title: An Escherichia coli mutant exhibiting temperature-sensitive ATP synthesis.
abstract: A mutant strain SM434 (ttr-3) of Escherichia coli that exhibits a temperature-sensitive Unc(succinate-nonutilizing) phenotype was characterized. The mutant allele ttr-3 was not linked to the ilvA gene, but was complemented by Fill carrying 81 min-91 min of the E. coli chromosome. The mutant strain SM434 exhibited resistance to N,N'-dicyclohexylcarbodiimide (DCCD) and a temperature-sensitive phenotype at the level of ATP synthesis, compatible with that of cell growth. These findings indicate that the mutant strain SM434 could carry a mutation (ttr-3) in an unknown gene responsible for the energy-transduction system.
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000483:Alleles; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004351:Drug Resistance; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D010641:Phenotype; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D013696:Temperature
chemical_list: D013386:Succinates; D004024:Dicyclohexylcarbodiimide; D000255:Adenosine Triphosphate; D019802:Succinic Acid