title: Solubilization and characterization of surface antigenic components of Erysipelothrix rhusiopathiae T28.
abstract: The antigenicity of Erysipelothrix rhusiopathiae T28 (serotype 2) was investigated. Antigens were solubilized from the cell surface with detergents. By means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting it was shown that the molecular weight of the main antigenic component--a nonprotein--was 14,000 to 22,000. This major antigen was shown to be a polydisperse anionic polysaccharide located on the surface of E. rhusiopathiae. Affinity chromatography also revealed a number of immunologically active proteins with molecular weights of 78,000, 72,000, 68,000, and 48,000.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000276:immunology; D002846:Chromatography, Affinity; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D004888:Erysipelothrix / Q000276:immunology*; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D012995:Solubility
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D003902:Detergents