Effects of polynucleotide inhibitors on transcriptional events of influenza virions.
Polynucleotide inhibitors of the influenza virion transcriptase, which is activated in vitro by detergent, can be divided into two categories. Polyribonucleotides comprised of adenine and guanine bases or adenine and uracil bases inhibit initiation but not elongation events of transcription. In contrast, polyribonucleotides containing cytidine and uracil or inosine and uracil block both initiation and elongation. Effects on elongation are apparent on addition of polynucleotides at either 5 or 10 min post-initiation. Dose-response studies with these and other polynucleotides have shown that the concentrations causing 50% inhibition of transcription vary considerably with the most potent inhibitor being the modified polymer, poly(C,S4U10).
D009975:Orthomyxoviridae / Q000235:genetics* / Q000378:metabolism; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D011119:Polynucleotides / Q000037:antagonists & inhibitors*; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014771:Virion / Q000235:genetics* / Q000378:metabolism
D011119:Polynucleotides; D012333:RNA, Messenger; D012367:RNA, Viral