A transcription terminator in the 5' non-coding region of the tyrosyl tRNA synthetase gene from Bacillus stearothermophilus.
The 5' non-coding region of the tyrosyl-tRNA synthetase gene (tyrS) of Bacillus stearothermophilus is 324 nucleotides long. It contains a premature terminator and a strong promoter: these were identified in vitro by RNA run-off experiments and in Escherichia coli by construction of specific mutants. The terminator consists of a stem and loop structure followed by the string of T residues characteristic of rho-independent termination. This is preceded by another stem and loop structure which may permit the formation of an anti-terminator. Neither the promoter nor the premature terminator appears to be regulated by the tyrosyl-tRNA synthetase in vitro or in E. coli.
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D004926:Escherichia coli / Q000235:genetics; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014448:Tyrosine-tRNA Ligase / Q000235:genetics*
D000604:Amino Acyl-tRNA Synthetases; D014448:Tyrosine-tRNA Ligase