Improvements in production of cDNA libraries in lambda gtll. Detection of ligation inhibitor and separation of cDNA from free linkers.
A ligation assay was designed to determine if a ligation inhibitor is present in a Bio-Gel A-50-m column, the traditional tool used for separation of cDNA from free linkers during the lambda gtll cloning procedure. A new and simple electrophoresis method is also presented for performing this separation, which is rapid, efficient, and reduces the chance of carrying along a ligation inhibitor.
D002850:Chromatography, Gel; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI
D004247:DNA; D015246:Deoxyribonuclease EcoRI