A rapid enhanced chemiluminescent immunoassay for allergen-specific IgE antibodies.
An enhanced chemiluminescent immunoassay is described for the measurement of allergen-specific IgE antibodies. The assay is demonstrated for pollen from four grass species. A comparison was made between results obtained by this method and those obtained by the radioallergosorbent (RAST) procedure; a high degree of correlation (r = 0.95) was found for D. glomerata specific IgE. The assay is rapid and can be carried out in under 1 hour. The advantages of the luminescent assay as compared with the RAST procedure are discussed.
D000485:Allergens / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007073:Immunoglobulin E / Q000032:analysis*; D008163:Luminescent Measurements; D011058:Pollen; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*
D000485:Allergens; D007073:Immunoglobulin E