Altered heat-shock response in polyamine-depleted bacteria.
A polyamine-auxotrophic mutant of E. coli was cultivated in the presence or absence of putrescine and submitted to heat shock over 3 different ranges of temperature. In all cases, protein synthetic capacity measured in comparison to that of cultures at the preshift temperature was much higher in polyamine-depleted bacteria under thermic stress. Addition of putrescine only before the shift-up was able to restore gradually normal control of the relative protein synthetic capacity.
D001426:Bacterial Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D011700:Putrescine / Q000378:metabolism* / Q000494:pharmacology; D013462:Sulfur Radioisotopes
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine; D011700:Putrescine