Use of particulate antigen to enhance primary in vitro antibody responses to trinitrophenyl-keyhole limpet hemocyanin.
Methods are described for the efficient induction of primary anti-hapten antibody responses to trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH) in vitro. Mouse leukocytes stimulated in vitro with particulate (bentonite-adsorbed) TNP-KLH demonstrated 10-fold more IgM-secreting, plaque-forming cells (PFC) as measured by a hemolytic plaque assay than did leukocytes stimulated with soluble TNP-KLH. The T dependency of the enhanced antibody response was confirmed using highly enriched B and T lymphocyte populations. These methods should greatly facilitate studies regarding primary in vitro antibody responsiveness to T-dependent antigens.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D002352:Carrier Proteins / Q000276:immunology; D003470:Culture Media; D005260:Female; D006241:Haptens / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009578:Nitrobenzenes / Q000276:immunology*; D012995:Solubility; D014301:Trinitrobenzenes / Q000276:immunology*
D002352:Carrier Proteins; D003470:Culture Media; D006241:Haptens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin