title: Arginine auxotrophs of Candida albicans deficient in argininosuccinate lyase.
abstract: Auxotrophic mutants of Candida albicans FC18 were induced by a combination of treatments with nitrous acid and UV irradiation. Arginine (Arg-), histidine (His-) and methionine/cysteine (MetA-) auxotrophs were recovered by this means. The Arg- auxotrophs lacked active argininosuccinate lyase (EC 4.3.2.1), the enzyme catalysing the final step in arginine biosynthesis. Thus the locus may be designated arg-4. The mutant strains bearing this mutation did not form germ tubes unless the germination medium contained arginine.
mesh_terms: D001120:Arginine / Q000378:metabolism*; D056807:Argininosuccinic Aciduria; D002176:Candida albicans / Q000201:enzymology* / Q000528:radiation effects; D006639:Histidine / Q000378:metabolism; D008190:Lyases / Q000172:deficiency*; D009154:Mutation; D014466:Ultraviolet Rays
chemical_list: D006639:Histidine; D001120:Arginine; D008190:Lyases