An enzyme immunoassay for quantitation of a type 2 pneumocyte-specific surfactant-associated antigen in bronchoalveolar lavage fluid.
A sensitive, specific and reproducible assay for quantitation of a surfactant-associated antigen in mouse bronchoalveolar lavage fluid is described. The procedure employed was a non-equilibrium competitive enzyme immunoassay in which detection of unreacted antibody was significantly enhanced by the use of a second antibody and peroxidase-antiperoxidase as the reporter label. The sensitivity of the assay was such that bronchoalveolar lavage fluid had to be diluted 15 to 30-fold prior to assay. No interference by serum proteins or soluble tissue proteins was observed. This assay may provide in vitro quantitative assessment of the functional status of type 2 pneumocytes.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001826:Body Fluids / Q000276:immunology; D001980:Bronchi / Q000276:immunology; D005260:Female; D007124:Immunoenzyme Techniques; D008168:Lung / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011650:Pulmonary Alveoli / Q000276:immunology; D011663:Pulmonary Surfactants / Q000276:immunology*; D007507:Therapeutic Irrigation
D000941:Antigens; D011663:Pulmonary Surfactants