An animal model of antibody binding in cutaneous lupus.
To study antibody binding in cutaneous lupus, we used human skin grafted onto nude mice. By immunofluorescence examination, mice injected with anti-Ro (SS-A) sera from subacute cutaneous or neonatal lupus erythematosus patients showed evidence of human IgG deposited in the skin, while mice injected with anti-native DNA or normal sera did not. We present evidence that there is specific binding of anti-Ro (SS-A) antibodies to Ro (SS-A) antigen in the skin, and we propose that these antibodies may be directly involved in cutaneous disease.
D000328:Adult; D000818:Animals; D000974:Antibodies, Antinuclear / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001324:Autoantigens / Q000032:analysis / Q000528:radiation effects; D001666:Binding Sites, Antibody; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007231:Infant, Newborn; D008179:Lupus Erythematosus, Discoid / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D012867:Skin / Q000276:immunology / Q000528:radiation effects; D016038:Skin Transplantation; D014183:Transplantation, Heterologous; D014466:Ultraviolet Rays; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D001324:Autoantigens; D007074:Immunoglobulin G; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human; C035356:SS-A antibodies