Localization of elongation factor Tu on the ribosome.
The EF-Tu-binding center of the E. coli ribosome has been localized by immunoelectron microscopy after cross-linking of the specific EF-Tu X 70 S ribosomal complex with dimethylsuberimidate. EF-Tu has been found to be in contact with the 50 S subunit in the region of the L7/L12 stalk and with the 30 S subunit in the upper part of its body on the side opposite the top of the ledge (the platform). The EF-Tu position on a model of the 70 S ribosome is presented.
D001666:Binding Sites, Antibody; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004120:Dimethyl Suberimidate / Q000494:pharmacology; D004926:Escherichia coli / Q000648:ultrastructure*; D007158:Immunologic Techniques; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008961:Models, Structural; D010444:Peptide Elongation Factor Tu / Q000276:immunology / Q000378:metabolism*; D012270:Ribosomes / Q000276:immunology / Q000378:metabolism*
D003432:Cross-Linking Reagents; D046911:Macromolecular Substances; D004120:Dimethyl Suberimidate; D010444:Peptide Elongation Factor Tu