In vitro metabolism of adrenocortical hormones by mammary glands of lactating rats. A comparative study.
A comparative study was made of the metabolism of tritium-labeled corticosterone, cortisol and aldosterone on incubation with minced mammary glands of lactating rats. The yield of total nonpolar (acylated) radiometabolites was highest for [3H]corticosterone, lowest for [3H]cortisol and intermediate for [3H]aldosterone. Unlike [3H]corticosterone, [3H]aldosterone yielded two 21-acyl derivatives (Metabolites I and II) in comparable amounts. Metabolite I (39%) was identified as [3H]aldosterone 21-oleate by isotope dilution analysis. Metabolite II (54%) could not be identified: it is intermediate in polarity between corticosterone 21-oleate and the less polar, corticosterone 21-stearate, and is distinctly less polar than the 21-palmityl, linoleoyl (and presumably also less polar than the arachidonyl) derivatives of aldosterone. The [3H]cortisol metabolites were not further investigated.
D000107:Acetylation; D000305:Adrenal Cortex Hormones / Q000378:metabolism*; D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D002855:Chromatography, Thin Layer; D003345:Corticosterone / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D006854:Hydrocortisone / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D010084:Oxidation-Reduction; D011247:Pregnancy; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains
D000305:Adrenal Cortex Hormones; D005227:Fatty Acids; D000450:Aldosterone; D003345:Corticosterone; D006854:Hydrocortisone