Separation of motile human spermatozoa by means of a glass bead column.
Fertility potential of semen depends upon the presence of viable and motile spermatozoa. A variety of techniques has been reported whereby motile spermatozoa are separated from semen for use in homologous insemination. Using a column of glass beads, we tested various sizes for spermatozoa-separating efficiency. This procedure is quick and simple and results in a significantly better recovery of motile and viable spermatozoa from poor-quality semen. Therefore it has the potential for use in intrauterine insemination for treating male factor infertility.
D002469:Cell Separation; D002845:Chromatography / Q000379:methods; D005898:Glass; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*