title: Patterning of the head in hydra as visualized by a monoclonal antibody. I. Budding and regeneration.
abstract: A monoclonal antibody, CP8, has been isolated which displays a position-specific binding pattern to epithelial cells of Hydra oligactis. Antibody binding is restricted to the head of adult animals. When a new head develops during the budding process, CP8 binding is present in the area which will form the head well before morphological signs of it. Similarly, following decapitation as a new head regenerates, CP8 label appears covering a domed area at the apical end of the regenerate before tentacles evaginate delineating the head. When bud development or regeneration is complete, CP8 label is restricted to the new head. Experiments indicate the appearance of CP8 label during the formation of a head correlates closely with the patterning events which result in the determination of the tissue to form a head. The usefulness of CP8 as a diagnostic tool for exploring the dynamics of head pattern formation in hydra is discussed.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006829:Hydra / Q000502:physiology*; D009024:Morphogenesis; D012038:Regeneration; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal