[Micromethod of leukocyte typing using monoclonal antibodies and indirect immunofluorescence: cell adsorption on support slides treated with poly-L-lysine].
A micromethod of indirect immunofluorescence with monoclonal antibodies to study surface markers of leukocytes and precursors, is described. This method consists of attaching non specifically living cells on a slide, previously treated with a high molecular weight polymer (L-lysin). The advantage of this method consists of using only 1.10(4) cells by typage, and low amounts monoclonal antibodies (twenty to forty less). In addition, this common method can be used in most biology laboratories.
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood; D007962:Leukocytes / Q000145:classification*; D011107:Polylysine
D000911:Antibodies, Monoclonal; D011107:Polylysine
Une microméthode pour le typage leucocytaire par les anticorps monoclonaux en immunofluorescence indirecte: adsorption cellulaire à des lames supports traitées par la poly-L-lysine.