A novel polymorphism of human complement component C3 detected by means of a monoclonal antibody.
A mouse monoclonal antibody, HAV 4-1, obtained after immunization of a BALB/c mouse with purified C3F, detected a novel genetic polymorphism of human complement component C3 in a simple immunoblotting system. The frequency of HAV 4-1-positive genes was 20.1%. Reactivity of HAV 4-1 was closely related to C3F, but certain individuals with the C3F allele did not react with HAV 4-1. Conversely, certain C3S homozygous individuals did react with HAV 4-1. The polymorphism detected by this monoclonal antibody is therefore different from the previously described polymorphism based on charge differences.
D000483:Alleles; D000911:Antibodies, Monoclonal; D003176:Complement C3 / Q000235:genetics* / Q000276:immunology; D005787:Gene Frequency; D006801:Humans; D007163:Immunosorbent Techniques; D010375:Pedigree; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D003176:Complement C3