[Ribosomal protein S1 in the complex of E. coli ribosomal subunit 30S with phage MS2 RNA interacts with internal region of the replicase gene].
The MS2 RNA fragments bound to ribosomal protein S1 within the complex of MS2 RNA with 30S ribosomal subunit have been isolated using a specially developed procedure and sequenced by the base-specific enzymatic method. The S1-binding site on MS2 RNA was identified as UUUCUUACAUGACAAAUCCUUGUCAUG and mapped within the replicase gene at positions 2030-2056. This finding suggests that ribosome-MS2 RNA interaction involves at least two different regions of the phage RNA--the internal region of the replicase gene (S1-binding site) and ribosome-binding site of the coat protein gene. The possible spatial proximity between these two regions is discussed.
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005814:Genes, Viral; D011777:Q beta Replicase / Q000235:genetics*; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D012367:RNA, Viral; D012269:Ribosomal Proteins; C015494:ribosomal protein S1; D011777:Q beta Replicase; D012316:RNA Nucleotidyltransferases
Ribosomnyĭ belok S1 v sostave kompleksa 30S ribosomnoĭ subchastitsy E. coli s RNK faga MS2 vzaimodeĭstvuet s vnutrennim raĭonom gena replikazy.