title: Stimulation by thyroid hormone of renin mRNA in mouse submandibular gland.
abstract: Renin in the female mouse submandibular gland (SMG) is known to increase in response to thyroid hormone. In the present study a 500 base-pair renin cDNA was used to quantify renin mRNA, the product of transcription of the Ren-2 gene, in the female mouse SMG in response to thyroid hormone to assess more directly the nature of stimulation of renin biosynthesis. After daily injection for 1 wk renin mRNA increased from control values of 36 +/- 3 SE ng/g tissue to 212 +/- 41 with triiodothyronine (T3; P less than 0.005) and to 217 +/- 33 with thyroxine (T4; P less than 0.005). Treatment of mice with propylthiouracil (PTU) decreased renin mRNA to 1.2 +/- 0.3 (P less than 0.005) and after subsequent injection of T3 for 1 wk renin mRNA increased to 289 +/- 35 ng/g tissue. The time course of the response to a single injection of thyroid hormone indicated a rapid response with significant increases in renin mRNA by 1 h, reaching values 3.5 +/- 0.4 times control (P less than 0.005) by 5 h. Such rapid effects are consistent with a direct nuclear action of thyroid hormone in stimulating transcription of the Ren-2 gene in mouse submandibular gland or in stabilization of nuclear precursor renin mRNA or both.
mesh_terms: D000818:Animals; D005260:Female; D007700:Kinetics; D051379:Mice; D011441:Propylthiouracil / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D012083:Renin / Q000235:genetics*; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D013974:Thyroxine / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D014284:Triiodothyronine; D011441:Propylthiouracil; D012083:Renin; D013974:Thyroxine