Two-dimensional electrophoresis of yeast histones on cellogel strips and polyacrylamide gels.
For a quick analytical identification of histones a two-dimensional electrophoretic system has been developed. First the proteins are separated on cellulose acetate strips in alkaline buffer. Then they are reelectrophoresed in a second dimension on polyacrylamide gels either with sodium dodecyl sulfate or in the presence of urea at pH 2.7. The technique is demonstrated with yeast and calf thymus histones.
D000818:Animals; D002417:Cattle; D004586:Electrophoresis / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013950:Thymus Gland / Q000032:analysis
D006657:Histones; D012967:Sodium Dodecyl Sulfate