title: Genetic analysis of DNA repair in Aspergillus: evidence for different types of MMS-sensitive hyperrec mutants.
abstract: To identify genes which affect DNA repair and possibly recombination in Aspergillus nidulans, mutants hypersensitive to methyl methanesulphonate (MMS) were induced with ultraviolet light (UV) or gamma-rays. About half of them contained associated translocations and many were hypersensitive to UV and/or defective in meiosis. Two are alleles of the known uvsB gene while most others define new genes. In addition, among available uvs mutants many were found to be MMS-sensitive. Some of the various uncharacterized ones were identified as alleles of known uvs, but 5 of them were mapped in 2 new genes, uvsH and uvsJ. To identify functional and epistatic groups, mutants from each uvs gene were tested for effects on recombination and mutation, and double mutant uvs strains were compared for UV survival to their component single mutant strains. 3 epistatic pairs were identified, (1) uvsF and H, (2) uvsB and D, and (3) uvsC and E. Conclusive interpair tests were difficult, because such double mutant combinations were frequently lethal or nearly so. The first pair, uvsF and H, shared some of the properties of excision-defective mutants, both uvs being very highly sensitive to UV for mutation as well as survival. But unlike such mutants, uvsH was also sensitive to gamma-rays and defective in meiosis. Both uvs showed normal levels of meiotic recombination, but greatly increased spontaneous mitotic crossing-over, being the most "hyperrec" types among all uvs. The second pair, uvsB and uvsC, which was similarly hyperrec showed only slight increases of UV-induced mutation (less than 2-fold). As a main effect, these uvs caused very high frequencies of unbalanced, unstable segregants from diploid conidia (30 X), but few of these were recognizable aneuploids. The third pair, uvsC and E, which are known to be rec- for gene conversion, caused reduced mitotic crossing-over in diploids and increased levels of haploid segregants. These mutants are spontaneous mutators, but showed less UV-induced mutation than wild-type controls.
mesh_terms: D000483:Alleles; D000782:Aneuploidy; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D004260:DNA Repair; D004271:DNA, Fungal / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000528:radiation effects; D004843:Epistasis, Genetic; D005720:Gamma Rays; D005800:Genes, Fungal / Q000187:drug effects / Q000528:radiation effects; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
chemical_list: D004271:DNA, Fungal; D008741:Methyl Methanesulfonate