Insulin-like molecules in Momordica charantia seeds.
Decorticated Momordica charantia seeds were extracted and processed by a method which was developed originally for the purification of insect and annelid insulins. Essentially, the method entailed HCl--ethanol extraction, neutralization with NH4OH, gel filtration on Sephadex G-50, ion exchange chromatography on CM Sepharose CL-6B and desalting on Sephadex G-10. Of the seven fractions collected, three fractions were obtained with antilipolytic and lipogenic activities in isolated adipocytes and one fraction with only lipogenic activity. The data indicate that molecules with insulin-like bioactivity are present in Momordica charantia seeds.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002681:China; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006461:Hemolysis / Q000187:drug effects; D007328:Insulin / Q000032:analysis* / Q000494:pharmacology; D050356:Lipid Metabolism; D010946:Plants, Medicinal / Q000032:analysis*
D007328:Insulin