T-cell detection with monoclonal antibody T101 kits.
A solid-phase immunoadsorption procedure (Quantigen T&B cell kit; Bio-Rad Laboratories, Richmond, Calif.) employing monoclonal antibody T101 detected mean percentages of peripheral blood T cells comparable to those obtained by rosetting with sheep erythrocytes, while lower values were obtained with an indirect immunofluorescence procedure (Cytotag T&B cell kit; Hybritech, Inc., San Diego, Calif.) employing the same antibody. Therefore, T101 binding appears to be more easily detected by solid-phase immunoadsorption than by immunofluorescence microscopy.
D000911:Antibodies, Monoclonal; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification*
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic