A low molecular weight growth inhibitor secreted in cultures of chicken embryo fibroblasts.
We report evidence for the occurrence of a novel type of growth inhibitor in conditioned medium of chicken embryo fibroblasts which inhibits both DNA synthesis and cell proliferation. The inhibitor does not compete with the growth factors present in the serum and its effect on the target cells is non-cytotoxic and reversible. The kinetics of the inhibition of thymidine incorporation indicate that the inhibitor acts in the S-phase of the cell cycle. Comparison of the chemical and physico-chemical properties of the inhibitor with other, recently described growth inhibitors of fibroblasts has demonstrated that it is different in many respects. The inhibitor has a Mr of less than 2000, is protease resistant, and acid- and heat-stable.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects; D005347:Fibroblasts / Q000378:metabolism*; D006131:Growth Inhibitors / Q000378:metabolism*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism
D003470:Culture Media; D006131:Growth Inhibitors; D010447:Peptide Hydrolases