title: Effect of tunicamycin on thiamine transport in Saccharomyces cerevisiae.
abstract: The activity of thiamine transport in Saccharomyces cerevisiae was decreased by the treatment with tunicamycin without affecting the growth of yeast cells. Although the total activity of a soluble thiamine-binding protein in yeast periplasm, which is known to be a glycoprotein, was decreased by tunicamycin treatment, the activity of thiamine uptake by yeast protoplasts was inhibited as much as by whole cells. Furthermore, tunicamycin decreased the activity of the membrane-bound thiamine-binding protein in a dose dependent way and in parallel with the thiamine transport activity. These findings suggested that the membrane-bound thiamine-binding protein is a glycoprotein which plays a functional role in thiamine transport in S. cerevisiae.
mesh_terms: D000135:Acid Phosphatase; D001693:Biological Transport, Active / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives*; D011523:Protoplasts / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013831:Thiamine / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D002352:Carrier Proteins; D029701:Saccharomyces cerevisiae Proteins; C026989:thiamine-binding protein; D014415:Tunicamycin; D003513:Cycloheximide; D000135:Acid Phosphatase; C488913:PHO3 protein, S cerevisiae; C488916:PHO5 protein, S cerevisiae; D005944:Glucosamine; D013831:Thiamine