Immunohistochemical localization of neurofilaments and neuron-specific enolase in 29 cases of neuroblastoma.
Twenty-nine neuroblastomas have been examined with the use of rabbit antibodies specific for each of the three neurofilament polypeptides, with a monoclonal antibody specific for the NF-L polypeptide, and with a rabbit antibody specific for neuron-specific enolase. When frozen material was used, all neuroblastomas were positive with the neurofilaments antibodies. When alcohol-fixed paraffin-embedded material was used, neurofilament staining was weaker and the fixation procedure appeared to destroy the epitopes recognized by the NF-L antibodies preferentially. Although all neuroblastomas were positive for neurone-specific enolase, so were two rhabdomyosarcomas, suggesting that NSE is not an appropriate marker to distinguish the different small blue cell tumors of children.
D000293:Adolescent; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D003599:Cytoskeleton / Q000648:ultrastructure*; D005260:Female; D006651:Histocytochemistry; D006652:Histological Techniques; D006801:Humans; D007120:Immunochemistry; D007223:Infant; D007382:Intermediate Filaments / Q000648:ultrastructure*; D008297:Male; D009447:Neuroblastoma / Q000201:enzymology / Q000648:ultrastructure*; D010751:Phosphopyruvate Hydratase / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits
D000906:Antibodies; D010751:Phosphopyruvate Hydratase