Use of D-lactic acid measurements in the diagnosis of bacterial infections.
This study explored the use of D-lactic acid as a marker for bacterial infections. D-Lactic was produced by frequently encountered human bacterial pathogens under anaerobic growth conditions; Bacteroides fragilis produced the largest amount. Orally administered D-lactic acid was absorbed from the intestines of rats and later found in measurable quantities in the blood and urine. Eunephric and anephric rats that received D-lactic acid intravenously showed similar quantities of this metabolite in the blood. These quantities are consistent with the distribution of D-lactic acid to total body water. Isolated liver and lung tissues from rats did not metabolize or produce D-lactic acid. Rats with experimentally induced, sublethal klebsiella peritonitis had D-lactic acidemia of 0.2 mM and 25.6 mM at 0 and 6 hr of infection, respectively. In a normal human, D-lactic acid was detected in the urine and blood after a subcutaneous injection of D-lactic acid, and pharmacokinetics of elimination similar to those of rats were found.
D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001424:Bacterial Infections / Q000175:diagnosis* / Q000378:metabolism; D001441:Bacteroides fragilis / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007710:Klebsiella Infections / Q000097:blood; D007711:Klebsiella pneumoniae / Q000378:metabolism; D007773:Lactates / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D019344:Lactic Acid; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D010538:Peritonitis / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains
D007773:Lactates; D019344:Lactic Acid