title: Factors affecting the yield of viable cells of Corynebacterium pseudotuberculosis in a liquid medium.
abstract: Growth of Corynebacterium pseudotuberculosis in an undefined liquid medium was studied with the aim of obtaining uniform, single-cell suspensions of the organism suitable for experimental infection. Adequate yields of viable cells were shown to be dependent on a fermentable substrate and a buffering system in a medium containing Tween 80. The development of Nutrient Tween 80 Broth for the culture of C. pseudotuberculosis is considered to be a significant advance particularly for studies involving animal inoculation.
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D003352:Corynebacterium / Q000254:growth & development* / Q000302:isolation & purification; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008198:Lymph Nodes / Q000382:microbiology; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology
chemical_list: D003470:Culture Media