title: [Islet cell surface autoantibodies: their immunoenzyme analysis using rat target cells].
abstract: A hard phase immunoenzymatic system for primary screening and subsequent quantitative determination of autoimmunobodies to the surface of islet cells (ASIC) was worked out using rat target cells. ASIC organ-specificity was confirmed by the absence of crossreactions with rat hepatocytes and splenocytes. The determination of the upper normal bound with 99% confidence interval combined with a high sensitivity of the method allowed an objective and significant assertainment of ASIC-positive sera even with a low level of antibodies. ASIC quantitative characteristic is their titer determined with the help of the intersection of a titration curve with the upper normal bound. ASIC were detected approximately in half of the patients with insulin-dependent diabetes of a duration of maximum 5 years and were not detected in the patients with insulin-dependent diabetes compensated with diet and sugar lowering drugs using the above method.
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D007515:Islets of Langerhans / Q000276:immunology*; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D015478:Antigens, Heterophile; D001323:Autoantibodies; D007074:Immunoglobulin G
vernacular_title: Autoantitela k poverkhnosti ostrovkovykh kletok podzheludochnoĭ zhelezy: immunofermentnoe opredelenie s ispol'zovaniem kletok-misheneĭ krys.