DNA-repair characterization of cdc40-1, a cell-cycle mutant of Saccharomyces cerevisiae.
The cell-cycle specific mutation cdc40-1, which has been previously shown to be sensitive to MMS at the restrictive temperature, was further characterized as a DNA-repair-deficient mutation. cdc40-1 mutants shown only slight sensitivity to UV irradiation. Double mutant studies shown that rad6-l is epistatic to cdc40-1 with respect to sensitivity to UV irradiation and MMS. rad50-1 is epistatic to cdc40-1 with respect to MMS sensitivity in G1 stationary cells, but not in logarithmic cultures. An additive effect is seen between cdc40-1 and rad50-1 with respect to UV irradiation. cdc40-1 mutants are defective in UV-induced mutagenesis at the restrictive temperature. UV-induced levels of recombination are normal at both temperatures, while MMS-induced recombination is enhanced at the restrictive temperature.
D000483:Alleles; D002453:Cell Cycle; D004260:DNA Repair / Q000528:radiation effects; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D009154:Mutation; D011995:Recombination, Genetic / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D013696:Temperature; D014466:Ultraviolet Rays
D004271:DNA, Fungal