title: Pitfalls in immunocytochemistry with special reference to the specificity problems in the localization of neuropeptides.
abstract: In this review, the different types of specificity in immunocytochemistry (ICC) are discussed. Some examples of misinterpretations on the basis of ICC results are given. A strategy is proposed to assess the specificity of antisera and to test them again after purification.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001127:Arginine Vasopressin / Q000378:metabolism; D005453:Fluorescence; D007031:Hypothalamus / Q000378:metabolism; D007120:Immunochemistry / Q000379:methods*; D007163:Immunosorbent Techniques; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009481:Neurophysins / Q000378:metabolism; D010121:Oxytocin; D010544:Peroxidases / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D012995:Solubility; D013003:Somatosensory Cortex / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D009481:Neurophysins; D011498:Protein Precursors; D014667:Vasopressins; D001127:Arginine Vasopressin; D010121:Oxytocin; D009074:Melanocyte-Stimulating Hormones; D010544:Peroxidases