Regions of SV40 large T antigen necessary for oligomerization and complex formation with the cellular oncoprotein p53.
The simian virus 40 (SV40) T antigen is composed of 708 amino acids and forms monomers and various oligomers and, in small amounts, heterologous complexes with the cellular oncoprotein p53 (T-p53). Using SV40 mutants coding for T antigen fragments which are either deleted in the N-terminal half or truncated by various lengths at the C-terminal end, we found that a region between amino acids 114 and 152 and a C-terminal region up to amino acid 669 are essential for the formation of high Mr oligomers of T antigen. Furthermore, only the C-terminal end up to amino acid 669 is essential for T-p53 complex formation but not the N-terminus up to amino acid 152.
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D010750:Phosphoproteins / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D016159:Tumor Suppressor Protein p53
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D011108:Polymers; D016159:Tumor Suppressor Protein p53