title: Human luteinizing hormone-releasing hormone gene (LHRH) is located on short arm of chromosome 8 (region 8p11.2----p21).
abstract: Luteinizing hormone-releasing hormone (LHRH) is synthesized by hypothalamic neurons and affects the release of gonadotropic hormones from the anterior pituitary gland. A cDNA clone encoding the human LHRH precursor molecule was used to assign the LHRH gene to a human chromosome by in situ hybridization and Southern blot analysis. Metaphase spreads from two normal individuals were hybridized with 3H-labeled LHRH-specific sequence. Of 120 cells analyzed, 33 had silver grains over the p11.2----p21 bands of chromosome 8. No other chromosomal site was labeled above background, indicating the presence of a single site for LHRH sequences in the human genome. Independent confirmation for this location of the human LHRH gene on chromosome 8p was provided by analysis of DNA from human X Chinese hamster somatic cell hybrids. DNA samples were digested with EcoRI, blotted, and hybridized with the 32P-labeled human LHRH precursor cDNA probe. The single 11.5-kb human-specific band was detected only in hybrids containing human chromosome 8. Also, hybridization was observed in DNA from hybrids in which a portion of human chromosome 8 (region 8pter----8q21) had been spontaneously translocated onto a Chinese hamster chromosome.
mesh_terms: D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005796:Genes; D005819:Genetic Markers; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization
chemical_list: D005819:Genetic Markers; D007987:Gonadotropin-Releasing Hormone; D004247:DNA