Surface expression of neural cell adhesion molecule in cultured bovine paraneurones: immunogold and immunoperoxidase methods compared.
The D2 neural cell adhesion molecule immunolabelling patterns of cultured bovine chromaffin cells are compared at the ultrastructural level, using the indirect immunohistochemical method with the secondary antibody labelled with either 17 or 5 nm gold particles or horseradish peroxidase (HRP). With HRP-conjugated antibody an apparently continuous surface staining was produced, while with 17 nm gold particles relatively large areas of membrane remained unlabelled; 5 nm particles gave a pattern more closely resembling the localization obtained with HRP. These differences are explained by differences in steric hindrance inherent to each method.
D000313:Adrenal Medulla / Q000276:immunology*; D000818:Animals; D000954:Antigens, Surface; D002417:Cattle; D015815:Cell Adhesion Molecules; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D006046:Gold; D007118:Immunoassay / Q000379:methods*; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D010316:Particle Size
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D006046:Gold