Purification and characterization of bacteriophage 9NA lysozyme.
Bacteriophage 9NA is a virulent phage of Salmonella typhimurium which induces a lysozyme in host cells toward the later stages of its multiplication. 9NA lysozyme has been purified about 1000 fold starting from the lysate of 9NA infected cells. The enzyme has an optimum pH between 7 and 8 and its activity is dependent on the ionic strength of the assay medium. Salts like NaCl and KCl are inhibitory to the lysozyme. Gram-negative cells act as better substrate for the lysozyme than do Gram-positive cells. The enzyme has a molecular weight of about 2.1 X 10(4) and rapidly loses its activity at temperatures higher than 45 degrees C. The properties of 9NA lysozyme have been compared with those of T4, lambda and P22 lysozymes.
D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008837:Micrococcus / Q000201:enzymology; D009113:Muramidase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D009994:Osmolar Concentration; D012483:Salmonella Phages / Q000201:enzymology*; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000254:growth & development; D013045:Species Specificity; D013379:Substrate Specificity
D009113:Muramidase