Cloning of a gene encoding choline transport in Saccharomyces cerevisiae.
By genetic complementation in a yeast choline transport mutant from a yeast gene library, we isolated plasmids encoding choline transport. The cloned plasmids contained a common 4.0-kilobase DNA fragment and also complemented an ethanolamine transport defect. The cloned sequence present in the yeast genome was possibly unique.
D001483:Base Sequence; D001692:Biological Transport; D002794:Choline / Q000378:metabolism*; D003001:Cloning, Molecular; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D005800:Genes, Fungal; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D004983:Ethanolamines; D019856:Ethanolamine; D002794:Choline