Hapten-specific unresponsiveness in mice. IV.--Significance of anti-hapten antibody responses elicited by E. coli lipopolysaccharides in tolerant mice.
The antibody response to lipopolysaccharide (LPS) and trinitrophenol-LPS (TNP-LPS) was investigated in mice after tolerance induction to chemically reactive hapten 2-4-6-trinitrobenzene sulphonic acid. The depression of the anti-TNP IgM response elicided by LPS was moderate and lasted 1 week. Depression of the response to TNP-LPS was severe and lasted 5 weeks. Tolerant mice given LPS 7 days after tolerance induction, at a time when it elicited a normal response, and challenged with TNP-LPS 2 weeks later, exhibited the same depression of their anti-TNP response as tolerant mice not given LPS. Furthermore, the anti-TNP IgM elicided by TNP-LPS in normal mice was of higher avidity than that observed with LPS. These results suggest that the anti-TNP response elicided by LPS cannot account for the overall immune status of tolerant mice.
D000818:Animals; D000917:Antibody Formation; D004926:Escherichia coli / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D007075:Immunoglobulin M / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL
D006241:Haptens; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; C048761:trinitrophenyl-lipopolysaccharide