A radioimmunocytological quantitative method for the rapid detection of ras oncogene p21 protein in mammalian cells.
In order to provide a sensitive and quantitative detection method of ras p21 at the cytological level, the monoclonal antibody Y13 259 and iodinated protein A were used to locate the ras protein in various mammalian cell lines. The subsequent autoradiograph can be analysed by a computer-assisted system which showed in these reported experiments that the relative levels of p21 detected in these cells corresponded to results obtained earlier using conventional biochemical methods.
D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D007457:Iodine Radioisotopes; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D011518:Proto-Oncogene Proteins