[Use of solid-phase immunoenzyme analysis for the serodiagnosis of pseudotuberculosis].
The diagnostic test system based on the solid-phase enzyme immunoassay (EIA) for the detection of antibodies to Yersinia pseudotuberculosis in the sera of patients with the use of Soviet-made preparations and reagents has been developed. The test has been performed in microchambers for immunological reactions, thus making it possible to decrease the consumption of reagents 10-20 times in comparison with the traditional technique with the use of plates. The results of the titration of 42 sera in EIA and in the passive hemagglutination test (PHAT) are indicative of the presence of positive correlation (r = 0.78; p less than 0.05) between antibody titers in EIA and PHAT. A fourfold or greater increase in antibody titers has been determined by means of EIA in 80% of cases and with the use of PHAT in 55% of cases. The minimum diagnostic titer yielded by EIA has been determined: 1:256.
D000907:Antibodies, Bacterial / Q000032:analysis; D005069:Evaluation Studies as Topic; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D012698:Serologic Tests / Q000379:methods; D013997:Time Factors; D015007:Yersinia / Q000276:immunology; D015009:Yersinia Infections / Q000175:diagnosis*; D015012:Yersinia pseudotuberculosis Infections / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G
Primenenie tverdofaznogo immunofermentnogo analiza dlia serodiagnostiki psevdotuberkuleza.