Methylation of DNA guanine via the 1-carbon pool in dimethylnitrosamine-treated rats.
Treatment of rats with radioactive methionine and nonradioactive dimethylnitrosamine resulted in the formation of radioactive 7-methylguanine in rat-liver DNA. By comparing the specific activity of administered [14C-Me]-dimethylnitrosamine to the specific activity of isolated 7-methylguanine it was determined that following 20 mg/kg dimethylnitrosamine DNA methylation via the 1-carbon pool may account for up to 30% of the total 7-methylguanine formed.
D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000378:metabolism*; D004128:Dimethylnitrosamine / Q000378:metabolism / Q000633:toxicity*; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D008745:Methylation; D011865:Radioisotope Dilution Technique; D051381:Rats; D011916:Rats, Inbred F344; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D006147:Guanine; C008450:7-methylguanine; D004247:DNA; D004128:Dimethylnitrosamine