Pitfalls in immunocytochemistry with special reference to the specificity problems in the localization of neuropeptides.
In this review, the different types of specificity in immunocytochemistry (ICC) are discussed. Some examples of misinterpretations on the basis of ICC results are given. A strategy is proposed to assess the specificity of antisera and to test them again after purification.
D000818:Animals; D000918:Antibody Specificity; D001127:Arginine Vasopressin / Q000378:metabolism; D005453:Fluorescence; D007031:Hypothalamus / Q000378:metabolism; D007120:Immunochemistry / Q000379:methods*; D007163:Immunosorbent Techniques; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009481:Neurophysins / Q000378:metabolism; D010121:Oxytocin; D010544:Peroxidases / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D012995:Solubility; D013003:Somatosensory Cortex / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism
D009419:Nerve Tissue Proteins; D009481:Neurophysins; D011498:Protein Precursors; D014667:Vasopressins; D001127:Arginine Vasopressin; D010121:Oxytocin; D009074:Melanocyte-Stimulating Hormones; D010544:Peroxidases