title: A new liquid medium without blood and serum for culture of hemoflagellates.
abstract: A liquid medium without blood or serum was developed for cultivation of hemoflagellates. To a basic LIT medium containing liver infusion broth and tryptose, a mixture of RPMI 1640 and Medium 199 was added. This combination permitted high parasite yields useful for biochemical and immunological studies.
mesh_terms: D000818:Animals; D001769:Blood; D006224:Cricetinae; D003470:Culture Media; D007893:Leishmania donovani / Q000254:growth & development* / Q000472:pathogenicity; D007894:Leishmania mexicana / Q000254:growth & development* / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000472:pathogenicity
chemical_list: D003470:Culture Media