title: Electrophysiological properties of cultured adult rat ventricular cardiac muscle cells.
abstract: Action and resting potential characteristics of isolated adult rat myocardial cells maintained in culture for 10-28 days are described. Resting potentials averaged -76.3 +/- 2 mV in 5 mM extracellular [K+] ([K+]o). Resting potentials changed by 54.3 mV/decade change in [K+]o for concentrations greater than 5 mM. The average maximum rate of rise of action potential (Vmax) was 117.7 +/- 10 V/s with overshoots of 34.6 +/- 2.5 mV. Action potential durations (APD) to 0 and -40 mV and full repolarization were 21.8 +/- 3.9, 36.3 +/- 6.0, and 206 +/- 16.9 ms respectively. Action potential configurations were qualitatively similar to those previously reported by others for rat myocardial tissue or freshly dissociated cells. Tetrodotoxin (4 micrograms/ml) decreased Vmax to less than 24 V/s and decreased overshoot and APD. Isoproterenol (10(-8) M) decreased APD with slight elevation of the overshoot. Verapamil (10(-5) to 10(-4) M) depressed overshoot and plateau while slowing the final phase of repolarization. Verapamil (10(-4) M) depressed upstroke velocity and blocked excitability. While APDs recorded from these cultured cells are apparently longer than those reported by others for rat myocardial APDs, the values of all other electrophysiological parameters recorded are within the limits previously reported for normal rat myocardial tissue. These data indicate that adult rat myocardial cells maintained in tissue culture for 10-28 days possess electrophysiological properties and responses to pharmacological agents similar to adult rat myocardial tissue or undamaged freshly isolated cells.
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques; D004594:Electrophysiology; D005260:Female; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006352:Heart Ventricles; D007545:Isoproterenol / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009206:Myocardium / Q000166:cytology; D051381:Rats; D013779:Tetrodotoxin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D013779:Tetrodotoxin; D014700:Verapamil; D007545:Isoproterenol