Type-I collagen production by human odontoblast-like cells in explants cultured on cyanoacrylate films. Electron-immunolocalization of fibronectin at cell/film interface.
Odontoblast-like cells derived from human tooth pulps were maintained in explant culture and grown either on glass coverslips only (used as control) or on glass coverslips coated with cyanoacrylate films. Ultrastructural and cyto-morphometric evidence showed that cells exposed to cyanoacrylate, in contrast to controls, display a significant decrease of rough endoplasmic reticulum and mitochondria. In addition, immunofluorescent staining and radioimmunoassays for type-I collagen suggested disturbances in production for the exposed cells. The use of anti-fibronectin antibodies with electron-microscopic immunoperoxidase-labelling demonstrated that the adherence of cells to cyanoacrylate can involve both adhesion plaques and fibronectin. These results therefore suggest that there were no apparent differences in the adhesion interaction of cells between glass and cyanoacrylate substrates.
D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D003094:Collagen / Q000096:biosynthesis*; D003470:Culture Media / Q000032:analysis; D003487:Cyanoacrylates / Q000494:pharmacology*; D005109:Extracellular Matrix; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005898:Glass / Q000494:pharmacology; D006801:Humans; D008854:Microscopy, Electron; D009804:Odontoblasts / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D011863:Radioimmunoassay; D014070:Tooth / Q000166:cytology*
D003470:Culture Media; D003487:Cyanoacrylates; D005353:Fibronectins; D003094:Collagen