Hydrophobic interaction chromatography of autoproteolysis products of human seminal plasma.
Hydrophobic interaction chromatography was employed for the fractionation of the glycopeptide mixture obtained after autoproteolysis and delipidization treatment of normal human seminal plasma samples. This sequence of procedures led to the elimination of highly hydrophobic components and to the concentration of the carbohydrate-rich fractions. A partial separation between the O- and N-glycosidic-linked oligopeptides was also achieved after the chromatographic step.
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D008297:Male; D012661:Semen / Q000032:analysis*
D000596:Amino Acids; D006020:Glycopeptides