Extent of survival and vascularization of adult superior cervical sympathetic or nodose ganglia transplanted into the septal nuclei or choroid fissure of adult rats.
Adult superior cervical sympathetic ganglia were auto-transplanted, and adult nodose ganglia were homografted into the septal nuclei or the choroid fissure of adult Wistar rats. At times from 4 h to 9 weeks after operation, the distribution of surviving transplanted neurons was compared with the development of the transplant vascularization, as visualized by transcardial Indian ink filling of the host vascular system. Within 24 h, the ganglionic neurons and Schwann cells of the interior of the transplants in both sites were necrotic. The surviving neurons and Schwann cells formed a shell, occupying those areas of the transplant periphery which were in direct contact with the host circulation. Occasional ink-filled vessels were evident at this time in transplants in the choroid fissure, but there were none in the septal nuclei, where vessels did not appear until the next day. Blood vessels reached the centre of the ganglia by 3-4 days in the choroid fissure and one week in the septal nuclei, the finest diameter capillaries forming last. At longer survivals there was a slow loss of neurons, notable between 1 and 2 months, and leading progressively (especially in the septal transplantation site) to the disappearance of all but a very small number of ganglionic neurons. The general findings were similar for both types of ganglion, and in both sites, but the initial cell loss was much greater for both types of ganglia in the septum (over 90%) as compared with about a 50% loss in the choroid fissure. The initial rapid cell loss was probably a result of ischaemia. The subsequent, slow progressive loss may be associated with failure to make or receive neuronal connections, or the absence of appropriate growth factors.
D000818:Animals; D002560:Cerebrovascular Circulation; D005260:Female; D005728:Ganglia, Sympathetic / Q000098:blood supply / Q000502:physiology / Q000637:transplantation*; D006085:Graft Survival; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology / Q000637:transplantation / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000502:physiology / Q000648:ultrastructure; D012686:Septal Nuclei / Q000098:blood supply / Q000502:physiology* / Q000648:ultrastructure; D013238:Stereotaxic Techniques; D013997:Time Factors