Ia antigens in plastic-embedded tissues: a post-embedding immunohistochemical study.
The aim of the present study was to establish a plastic embedding technique that makes possible the immunohistochemical demonstration of class II major histocompatibility complex (MHC) antigens (Ia antigens) in undecalcified joint tissues. Therefore a series of fixatives and dehydrating agents was tested for saving Ia immunoreactivity by post-embedding immunostaining of thin sections (2 microns) of rat tissues that had been embedded in glycol methacrylate (GMA), and by comparing with cryostat sections. An indirect immunoperoxidase and the avidin-biotin complex (ABC) technique were used. Combined with fixation by 4% formaldehyde, dehydration with GMA was found to give the best preservation of Ia antigenicity, followed by dehydration with ethylene glycol. The thinness of tissue sections facilitated the association of Ia antigens with different subcellular compartments in distinct cell populations. These various patterns are described.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D003890:Desiccation; D005404:Fixatives; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D009206:Myocardium / Q000276:immunology; D051381:Rats; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014018:Tissue Distribution
D005404:Fixatives; D000949:Histocompatibility Antigens Class II