title: Glycine neurons in the brain and spinal cord. Antibody production and immunocytochemical localization.
abstract: Antibodies were raised against glycine and they were specific for immunocytochemistry. Obtained from rabbits immunized with glycine conjugated to glutaryled protein-carriers, antisera were then purified by adsorption on the various glutaraldehyde-conjugated protein-carriers. Using a modified ELISA method, their specificity was determined in competition experiments between conjugated glycine and either non-conjugated glycine or other conjugated amino acids or derivatives, preincubated with anti-glycine antibodies. Calculated at half-displacement, the resulting cross-reactivity ratios showed conjugated glycine to be the best recognized compound. By revealing the presence of the majority of the glycine-containing cell bodies in the brainstem and spinal cord, immunocytochemical applications of glycine antibodies confirmed their use as specific tools for a better understanding of the role of glycine in the central nervous system.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000917:Antibody Formation; D002490:Central Nervous System / Q000378:metabolism*; D005998:Glycine / Q000276:immunology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D011817:Rabbits / Q000276:immunology; D051381:Rats
chemical_list: D000906:Antibodies; D005998:Glycine