Rapid identification of gram-negative bacilli from blood cultures.
Blood-culture broths showing macroscopic or radiometric evidence of growth of gram-negative bacilli were examined by a rapid automated bacterial identification system. A differential centrifugation technique was developed to prepare suitable inocula. The identification results obtained were confirmed by the API 20E method, with single colonies of the strains isolated 24 h later. Of 90 organisms tested, seven did not give the same identification by the two systems. With the rapid automated technique a presumptive identification of gram-negative bacilli can be made 24 h earlier than by more conventional methods.
D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology; D002498:Centrifugation; D004755:Enterobacteriaceae / Q000145:classification; D006090:Gram-Negative Bacteria / Q000145:classification*; D006801:Humans; D011549:Pseudomonas / Q000145:classification; D018805:Sepsis / Q000382:microbiology*; D013997:Time Factors