Selection and performance of monoclonal and polyclonal antibodies in an IgM antibody capture enzyme immunoassay for rubella.
Monoclonal anti-human IgM and anti-rubella antibodies were prepared and tested in an IgM capture enzyme immunoassay (MACEIA) for rubella-specific IgM and compared with polyclonal reagents. Assay sensitivity was increased with monoclonal antibodies resulting in the improved discrimination of adult sera with low levels of specific IgM. Despite high IgM binding, interference by IgM anti-Ig was not a major problem. The use of monoclonal antibodies allowed assay simplification by the simultaneous rather than sequential addition of antigen and conjugate. Although comparable results were obtained with 33 test samples in the sequential and simultaneous MACEIA, the specificity and sensitivity of this modification requires further evaluation.
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D007457:Iodine Radioisotopes; D012412:Rubella virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007075:Immunoglobulin M; D007457:Iodine Radioisotopes