Development of mouse embryos in vitro is affected by strain and culture medium.
One-cell and two-cell embryos from three random-bred strains of mice--CF1, Dub:(ICR), and CFW (Swiss-Webster)--were cultured to the blastocyst stage in Spindle's, Earle's, Ham's F10, Whittingham's T6, or Hoppe and Pitts' medium. CFW embryos were more successful than CF1 and Dub:(ICR) embryos in developing to the blastocyst stage in all five media. Dub:(ICR) and CFW two-cell embryos showed the best development in Spindle's, Whittingham's T6, and Hoppe and Pitts', whereas CF1 two-cell embryos were most successful in developing in Hoppe and Pitts' medium. Similar results were obtained with one-cell embryos, although fewer developed to the blastocyst stage, and T6 rather than Hoppe and Pitts' medium sustained the best development of CF1 one-cell embryos. For all strains, the least successful development was in Ham's F10, but CFW embryos did show good development in this medium. In addition to the effects of various media on mouse embryo development, our results indicate that the strain of mouse used for the bioassay of media is of critical importance. Random-bred CFW (Swiss-Webster) mice are as suitable as a hybrid strain for this purpose.
D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology; D006063:Chorionic Gonadotropin; D003470:Culture Media; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D013045:Species Specificity
D006063:Chorionic Gonadotropin; D003470:Culture Media