Competitive enzyme-linked immunosorbent assay for cholera-related enterotoxins in Salmonella typhimurium.
We report a rapid competitive enzyme-linked immunosorbent assay to screen Salmonella typhimurium strains for cholera-related enterotoxin antigens. Polymyxin B extracts of bacterial cells from syncase-glucose broth cultures of 7 of 15 strains gave positive results. The specificity of the test was confirmed with known heat-labile-enterotoxin-positive and -negative Escherichia coli strains which gave significantly different values.
D000942:Antigens, Bacterial / Q000032:analysis; D001427:Bacterial Toxins / Q000032:analysis*; D002772:Cholera Toxin / Q000032:analysis; D004731:Endotoxins; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012486:Salmonella typhimurium / Q000032:analysis* / Q000276:immunology
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; C027952:salmonella toxin; D002772:Cholera Toxin