An enzyme-linked immunosorbent assay (ELISA) for anti-chlamydial secretory immunoglobulin A in guinea pig tears.
A method is described which permits the assay of specific secretory immunoglobulin A (sIgA) antibodies produced by guinea pigs in response to ocular infection with the guinea pig inclusion conjunctivitis strain of Chlamydia psittaci (GPIC agent). The enzyme-linked immunosorbent assay (ELISA) developed was shown to be more sensitive and less subjective than the micro-immunofluorescence assay as a means of assaying specific antibody.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002691:Chlamydophila psittaci / Q000276:immunology*; D003231:Conjunctivitis / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D013666:Tears / Q000276:immunology*
D000907:Antibodies, Bacterial; D007071:Immunoglobulin A, Secretory