Regulation of fructose-2,6-bisphosphate and glycogen synthesis by dichloroacetate and phenazine methosulphate in rat adipose tissue.
The effects of dichloroacetate and phenazine methosulphate on the content of fructose-2,6-bisphosphate and glycogenesis in incubated epididymal adipose tissue were examined. Both agents stimulated the synthesis of fructose-2,6-bisphosphate in the presence of glucose, the effect being higher in tissue from fasted-refed rats than in normal fed rats. Additions of dichloroacetate to the incubation medium also increased the incorporation of [U-14C]glucose into glycogen and this effect was additive with that of insulin. However phenazine methosulphate strongly depressed the insulin-dependent glycogen synthesis. These data are considered in relation to the increased rate of glucose metabolism known to occur in the presence of dichloroacetate and the stimulation of pentose phosphate pathway with phenazine methosulphate.
D000085:Acetates / Q000494:pharmacology*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D004822:Epididymis; D005635:Fructosediphosphates / Q000096:biosynthesis*; D006003:Glycogen / Q000096:biosynthesis*; D006598:Hexosediphosphates / Q000096:biosynthesis*; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology*; D010619:Phenazines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D005635:Fructosediphosphates; D006598:Hexosediphosphates; D007328:Insulin; D010619:Phenazines; D008773:Methylphenazonium Methosulfate; C027652:fructose 2,6-diphosphate; D006003:Glycogen; D003999:Dichloroacetic Acid