Structures of glycosphingolipids isolated from human embryonal carcinoma cells. The presence of mono- and disialosyl glycolipids with blood group type 1 sequence.
Structures of glycolipids present in the human embryonal carcinoma cell PA1, were elucidated by fast atom bombardment-mass spectrometry, methylation analysis, and exo- and endoglycosidase digestion. PA1 cells contain globotriaosylceramide, sialosylgangliotriaosylceramide, sialylated and nonsialylated lacto-N-neotetraosylceramide, and the following glycolipids with a blood group type 1 sequence: (formula; see text) The two former glycolipids, lacto-N-tetraosylceramide and sialosyllacto-N-tetraosylceramide, reacted with monoclonal antibodies, K21 and K4, respectively. K21 and K4 antigens are present in many of the human embryonal carcinoma cells but not in a variety of other cell lines, suggesting that sialylated but not fucosylated blood group type 1 sequences are characteristic markers for human embryonal carcinoma cells and malignant teratocarcinomas.
D000941:Antigens / Q000032:analysis; D001789:Blood Group Antigens / Q000276:immunology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D054278:Embryonal Carcinoma Stem Cells; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D006026:Glycoside Hydrolases; D006028:Glycosphingolipids / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D013058:Mass Spectrometry; D008745:Methylation; D014411:Neoplastic Stem Cells / Q000032:analysis*
D000941:Antigens; D001789:Blood Group Antigens; D006017:Glycolipids; D006028:Glycosphingolipids; C009255:sialoglycolipids; D006026:Glycoside Hydrolases