Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells.
Light-scattering properties of human T-lymphocyte subpopulations selected by immunofluorescence were studied. Based on differences in orthogonal light scattering, two subpopulations of T8-positive cells can be distinguished. The first population (T8a) has the same orthogonal light-scattering properties as T4-positive cells, whereas the orthogonal light scattering of the second population (T8b) was about 70% larger. Orthogonal light scattering of Leu7-positive lymphocytes resembles that of the T8b population. We have studied the occurrence of the subpopulation in healthy individuals and we discuss their possible functional identification. Light-scattering properties of lymphocyte subpopulations in two patients with B-cell chronic lymphatic leukemia suggest that this observation is of clinical interest.
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes; D002469:Cell Separation; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology; D008027:Light; D012542:Scattering, Radiation; D013601:T-Lymphocytes / Q000145:classification* / Q000166:cytology / Q000473:pathology
D000911:Antibodies, Monoclonal