Characterization of influenza virus neuraminidase with hemagglutinin activity and its comparison with that of viral neuraminidase.
The neuraminidase associated with the bifunctional protein, hemagglutinin-neuraminidase, of influenza virus has been characterized. The enzyme has a pH optimum of 4.5, does not require Ca2+ and is inactivated (98%) by incubation at 50 degrees C. The enzyme has a Km of 2.00 X 10(-3) M and 0.06 X 10(-3) M with the substrates 2-(3-methoxyphenyl)-N-acetylneuraminic acid and fetuin, respectively. The Ki is 400 X 10(-6) with the inhibitor 2-deoxy-2,3-dehydro-N-acetylneuraminic acid. The incorporation of labeled cysteine, valine and leucine in the hemagglutinin-neuraminidase protein is different from that of viral neuraminidase. A comparison of the properties of the neuraminidase associated with protein hemagglutinin-neuraminidase with that of viral neuraminidase or sialidase showed that the former is biochemically different and an antigenically distinct enzyme. The unique feature of the new enzyme is that it has the hemagglutinin activity as well. The two biological activities could not be separated from each other in all systems used. Apparently, protein hemagglutinin-neuraminidase is genetically transferable and it is detectable in a laboratory recombinant virus E-2971 (H3 Aichi X N7). These results suggest that protein hemagglutinin-neuraminidase is a unique surface protein of the influenza virus A/Aichi/2/68 (H3N2).
D004591:Electrophoresis, Polyacrylamide Gel; D015707:HN Protein; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D007158:Immunologic Techniques; D007700:Kinetics; D009439:Neuraminidase / Q000276:immunology / Q000302:isolation & purification*; D009975:Orthomyxoviridae / Q000201:enzymology* / Q000276:immunology; D011489:Protein Denaturation; D011995:Recombination, Genetic; D013379:Substrate Specificity; D014759:Viral Envelope Proteins / Q000276:immunology / Q000302:isolation & purification*
D015707:HN Protein; D006389:Hemagglutinins, Viral; D014759:Viral Envelope Proteins; D009439:Neuraminidase