Corneal transplantation using long-term cultured donor material.
Twenty-seven corneal transplantations with a mean duration of donor incubation of 14.3 days (group 1) and 36 transplantations with a mean duration of incubation of 29.8 days (group 2) were followed for 18 months. No significant difference was found in graft survival between the two groups (81 versus 80%). Almost all graft failures were found among pre-operatively defined high-risk cases, i.e. previously transplanted and/or vascularized recipient cornea. The profile of the corneal thickness curve was almost identical in the two groups, and normal values were reached in both. Visual acuity was 0.5 or better in 23% of the cases in group 1, and 35% in group 2. These figures were highly influenced by co-existing and complicating eye disease i.e. amblyopia, cataract, macular degeneration and glaucoma. The results show that 2 weeks and 4 weeks cultured cornea do not differ with respect to graft survival and thickness. The over all results indicate that long-term cultured donor material is suitable for corneal transplantation and fully comparable to material stored by other methods.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D016039:Corneal Transplantation; D005260:Female; D006085:Graft Survival; D006801:Humans; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D013997:Time Factors; D014019:Tissue Donors; D014021:Tissue Preservation / Q000379:methods*