A novel method for the synthesis of kyotorphin, Tyr-Arg, and 3H-Tyr-Arg, catalyzed by tyrosyl-tRNA synthetase from Bacillus stearothermophilus.
A novel method of dipeptide synthesis is described that can be carried out in aqueous solution and does not require complicated protecting and deprotecting procedures. An analgesic neuropeptide named kyotorphin, H-Tyr-Arg-OH, was synthesized from unprotected tyrosine and arginine in a new enzymatic reaction catalyzed by immobilized tyrosyl-tRNA synthetase from Bacillus stearothermophilus. The reaction could be a useful tool in the syntheses of radioisotope-labeled oligopeptides to be used in receptor binding assays. 3H-Kyotorphin was prepared by this method at a yield of 72% and could be used in receptor binding assays after a single chromatographic separation.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000700:Analgesics / Q000302:isolation & purification*; D001120:Arginine / Q000378:metabolism; D002384:Catalysis; D004723:Endorphins / Q000096:biosynthesis* / Q000302:isolation & purification; D004800:Enzymes, Immobilized; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000378:metabolism; D006868:Hydrolysis; D007553:Isotope Labeling; D013696:Temperature; D014443:Tyrosine / Q000378:metabolism; D014448:Tyrosine-tRNA Ligase / Q000378:metabolism*
D000700:Analgesics; D004723:Endorphins; D004800:Enzymes, Immobilized; C019448:kyotorphin; D014443:Tyrosine; D001120:Arginine; D000604:Amino Acyl-tRNA Synthetases; D014448:Tyrosine-tRNA Ligase