[The use of highly purified staphylococcal alpha-toxin in the immunochemical purification of antibodies].
A simple procedure is described for isolation of highly purified staphylococcal alpha-toxin (SAT) by means of sorption on macroporous glass beads and gel filtration. Various conditions were tested of affinity immunosorption of antibodies and SAT. Highly-porous polyacrylamide gel activated by glutaraldehyde was found as a readily available and selective immunosorbent. The immobilized antigen was stabilized using reduction of azomethyne bonds. Specific activity of antibodies was elevated as the SAT purification degree increased. Correlation between antihemolytic and antilethal activities was unaltered during the immunochemical purification of antibodies.
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000276:immunology* / Q000633:toxicity; D006460:Hemolysin Proteins; D006736:Horses; D007114:Immunization; D007163:Immunosorbent Techniques; D051379:Mice; D009500:Neutralization Tests; D013211:Staphylococcus aureus / Q000276:immunology*
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin
Ispol'zovanie vysokoochishchennogo stafilokokkovogo alpha-toksina dlia immunokhimicheskoĭ ochistki antitel.