title: Factors influencing invasion of erythrocytes by Plasmodium falciparum parasites: the effects of an N-acetyl glucosamine neoglycoprotein and an anti-glycophorin A antibody.
abstract: When schizont-infected erythrocytes were incubated with N-acetyl glucosamine coupled to bovine serum albumin (GluNAc-BSA), the number of new ring forms which appeared several hours later was reduced and the number of abnormal and unruptured schizont-infected erythrocytes was increased compared with controls, indicating that GluNAc-BSA prevents invasion by a toxic effect on schizonts rather than by receptor blockade. Invasion of erythrocytes by Plasmodium falciparum was inhibited by a monoclonal antibody against glycophorin A, but inhibition also occurred with P. knowlesi, a parasite that is known to invade independently of glycophorin A. Inhibition of invasion with anti-glycophorin A is unlikely to be related to receptor blockade and is probably related to decreased deformability of the erythrocyte membrane caused by the binding of this antibody. Previous studies suggesting that GluNAc-BSA and anti-glycophorin A antibodies inhibit invasion by receptor blockade should be reevaluated. Erythrocytes deficient in glycophorin C and band 4.1 were also resistant to invasion by both P. falciparum and P. knowlesi.
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal; D001798:Blood Proteins / Q000502:physiology; D003598:Cytoskeletal Proteins; D004910:Erythrocyte Membrane / Q000502:physiology; D004912:Erythrocytes / Q000469:parasitology*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006021:Glycophorins / Q000502:physiology; D006801:Humans; D008565:Membrane Proteins; D009479:Neuropeptides; D010963:Plasmodium falciparum / Q000502:physiology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D006021:Glycophorins; D008565:Membrane Proteins; C050080:N-acetylglucosamine-bovine serum albumin conjugate; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1; D012710:Serum Albumin, Bovine; D005944:Glucosamine; D000117:Acetylglucosamine