Synthesis and intracellular localization of chick acid alpha-glucosidase in chick erythrocyte-human fibroblast heterokaryons. A model system for the study of lysosomal enzyme synthesis.
The synthesis and localization of chick acid alpha-glucosidase has been studied in chick erythrocyte-human fibroblast heterokaryons. Monospecific antibodies raised against purified chick liver acid alpha-glucosidase were used. It was found that the acid alpha-glucosidase in the heterokaryons is of chick origin, and is localized in the same lysosomes as the human lysosomal enzymes. It is concluded that chick erythrocyte-human fibroblast heterokaryons provide a useful model system for the study of lysosomal enzyme synthesis and routing.
D000818:Animals; D002451:Cell Compartmentation; D002645:Chickens / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005959:Glucosidases / Q000378:metabolism*; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology* / Q000648:ultrastructure; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D010920:Placenta / Q000201:enzymology; D000520:alpha-Glucosidases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D005959:Glucosidases; D000520:alpha-Glucosidases