Rat (and mouse) monoclonal antibodies. V. A simple automated technique of antigen purification by immunoaffinity chromatography.
A technique to automate the purification of antigens by immunoaffinity chromatography is described. It is operated on a time basis and measurements of the optical density of the immunoaffinity column eluate are made at 280 nm. Immunoaffinity columns of Sepharose 4B coupled mouse or rat monoclonal antibody can be used for 150 or more cycles. From 23 to 120 mg of monoclonal antibody were purified per run. There is a potential application of this system to other forms of affinity chromatography.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000941:Antigens / Q000302:isolation & purification*; D001322:Autoanalysis; D002846:Chromatography, Affinity / Q000379:methods*; D007163:Immunosorbent Techniques; D051379:Mice; D051381:Rats
D000911:Antibodies, Monoclonal; D000941:Antigens