Monoclonal antibodies against Trichomonas vaginalis.
Spleen lymphocytes obtained from mice immunized with Trichomonas vaginalis ATCC 30001 were fused with P3-X63-Ag8-653 mouse myeloma in order to produce hybridoma-secreting antibodies against T. vaginalis associated antigens. Six hybridoma cloned cell lines were established; three produced IgG1, two produced IgG2a, and one produced IgM monoclonal antibody. These six monoclonal antibodies showed binding to seven isolated strains of T. vaginalis but did not bind to Giardia lamblia. Three of those monoclonal antibodies did not bind to Tritrichomonas foetus. These anti-trichomonal monoclonal antibodies should prove to be of great value as diagnostic and research reagents.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D005872:Giardia / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D051379:Mice; D009101:Multiple Myeloma; D013048:Specimen Handling; D013154:Spleen / Q000166:cytology; D014244:Trichomonas / Q000276:immunology; D014245:Trichomonas Infections / Q000175:diagnosis; D014247:Trichomonas Vaginitis / Q000175:diagnosis; D014246:Trichomonas vaginalis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M