title: The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli. A mutagenesis study.
abstract: Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of defined nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding.
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011072:Poly U / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012335:RNA, Ribosomal / Q000032:analysis*; D012343:RNA, Transfer / Q000032:analysis / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000032:analysis; D013329:Structure-Activity Relationship
chemical_list: D011506:Proteins; D012335:RNA, Ribosomal; D012346:RNA, Transfer, Amino Acyl; D011072:Poly U; D012343:RNA, Transfer