Efficient purification of mouse monoclonal antibodies from ascites fluid by medium-performance anion exchange chromatography.
Medium-performance anion-exchange chromatography was applied to the purification of murine IgG class monoclonal antibodies from ascites fluid. The separations were performed under mild conditions at pH 8 using relatively low sodium-chloride concentrations. Recoveries for monoclonal antibodies of subclasses IgG1, IgG2a, and IgG2b were about 90%. The IgG preparations were free of other ascites fluid proteins.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001201:Ascites / Q000276:immunology; D002852:Chromatography, Ion Exchange / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000145:classification / Q000302:isolation & purification; D007202:Indicators and Reagents
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007202:Indicators and Reagents