Isolation of glutathione-deficient mutants of the yeast Saccharomyces cerevisiae.
Glutathione-deficient (gsh-) mutants of the yeast Saccharomyces cerevisiae were isolated after UV treatment using MNNG as selective agent. For genetic and biochemical characterization 5 mutant strains were chosen which exhibited considerably decreased residual GSH contents varying from 2 to 6% of the wild-type levels. All 5 isolates showed a 2:2 segregation of the gsh-:GSH+ phenotypes alluding to a monogenic recessive mutation. Complementation analysis indicates that all gsh- mutants belong to one complementation group.
D005656:Fungal Proteins / Q000378:metabolism; D005978:Glutathione / Q000172:deficiency* / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D005656:Fungal Proteins; D005978:Glutathione