Laser-excited immunofluorometric assay of prolactin, with use of antibodies coupled to lanthanide-labeled diethylenetriaminepentaacetic acid.
We describe an immunofluorometric assay for prolactin based on lanthanide labeling of a monoclonal antibody and measuring time-resolved fluorescence. In this "sandwich"-type assay, the label (Eu3+) was bound to the second antibody by means of a simple, rapid method involving the anhydride of diethylenetriaminepentaacetic acid. To measure the photoluminescence of europium (or other lanthanides), we have developed a time-resolved fluorometer with a nitrogen laser as the pulsed excitation source. During the assay, the solid-phase antibody immobilized inside a polystyrene tube is incubated with the plasma sample and the second antibody in a one-step procedure. Results for 67 human plasmas correlated well (r = 0.98) with those by an immunoradiometric method.
D000911:Antibodies, Monoclonal; D005260:Female; D005455:Fluorescent Antibody Technique; D005470:Fluorometry / Q000295:instrumentation; D006801:Humans; D007118:Immunoassay; D007202:Indicators and Reagents; D007834:Lasers; D008297:Male; D008674:Metals, Rare Earth; D004369:Pentetic Acid; D011388:Prolactin / Q000097:blood*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D008674:Metals, Rare Earth; D004369:Pentetic Acid; D011388:Prolactin