Direct evidence for a coupling between synthesis and export of PhoS in E. coli.
The accumulation of pre-PhoS under conditions of PhoS overproduction has been previously described. It is now demonstrated that during the induction of PhoS, a delay in the completion of polypeptide chain elongation can be detected. This delay is related to the extent of jamming of export sites by pre-PhoS or by other exported proteins. These results suggest that a component required for completion of pre-PhoS polypeptide becomes limiting, being titrated by the excess of nascent chains bearing signal peptides. This component thus probably acts at an early step in the export pathway.
D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000032:analysis; D004926:Escherichia coli / Q000378:metabolism*; D005786:Gene Expression Regulation; D010441:Peptide Chain Elongation, Translational; D028044:Phosphate-Binding Proteins; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D013997:Time Factors
D002352:Carrier Proteins; D028044:Phosphate-Binding Proteins; D011498:Protein Precursors