Endotoxin-mediated pulmonary endothelial cell injury.
Infusion of endotoxin into sheep results in physiological and structural damage to the pulmonary endothelium. It is uncertain whether complement activation and granulocyte sequestration in the pulmonary microcirculation and the ensuing granulocyte migration into the interstitium seen with endotoxemia contribute to the endothelial damage. We have shown that infusion of complement-activated plasma into sheep, although causing the same degree of granulocyte sequestration in the lungs, results in only modest and transient endothelial damage. In addition, migration or chemotaxis of granulocytes across the endothelial layer of intimal explants is not accompanied by either structural evidence of endothelial damage or a detectable increase in vascular permeability. Such studies indicate that neither complement/granulocyte activation nor granulocyte migration across a vessel wall is entirely responsible for the severe endothelial damage seen with endotoxin. In vitro studies of bovine pulmonary endothelial monolayers indicate that endotoxin can cause direct damage to the endothelium; the damage is dose-dependent and more severe in the presence of serum. Structural studies show endothelial cell retraction, pyknosis, and sloughing. Prostacyclin production and lactic dehydrogenase release are increased, as are permeability to small solutes and hydraulic conductance across the endothelium. It seems that endotoxin can cause a direct injury to pulmonary endothelium but complement and granulocyte activation may enhance the damage.
D000818:Animals; D002199:Capillary Permeability; D002634:Chemotaxis, Leukocyte; D003167:Complement Activation; D004727:Endothelium / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D004731:Endotoxins / Q000633:toxicity*; D011464:Epoprostenol / Q000096:biosynthesis; D004926:Escherichia coli; D006098:Granulocytes / Q000473:pathology; D008168:Lung / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D010949:Plasma / Q000502:physiology; D012128:Respiratory Distress Syndrome / Q000473:pathology* / Q000503:physiopathology
D004731:Endotoxins; D011464:Epoprostenol