Immunoperoxidase localization of type X collagen in chick tibiae.
Type X collagen was prepared from medium of long-term cultures of embryonic chick tibiotarsal chondrocytes. Antibodies to type X collagen were raised and used in immunoperoxidase localization studies with embryonic and growing chick tibiotarsus. Strong anti-type X collagen reactivity was detected mainly in the region of hypertrophic chondrocytes, and to a lesser extent in the zone of calcified cartilage. No reactivity was detected in the proliferative zone nor the superficial layer of the cartilage growth plate. These results suggest that type X collagen may play a key role in matrix calcification during growth and development of the skeletal system.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002356:Cartilage / Q000166:cytology; D002642:Chick Embryo; D003094:Collagen / Q000032:analysis* / Q000145:classification; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D011817:Rabbits; D013977:Tibia / Q000032:analysis* / Q000166:cytology
D007106:Immune Sera; D003094:Collagen