An improved procedure for the purification of Trypanosoma cruzi (Chagas, 1909) metacyclics from the insect vector.
We have developed an improved procedure for isolating and purifying the metacyclic trypomastigote form of Trypanosoma cruzi from infected Triatoma infestans. The procedure was simple, did not require time-consuming removal of the insect gut, and gave a good recovery of metacyclics. Purification involved centrifugal flotation of the parasites in Percoll followed by diethylaminoethyl cellulose column chromatography. The resulting purified metacyclics exhibited no loss of infectivity when assayed in mice as compared to metacyclics taken directly from the insects.
D000818:Animals; D007303:Insect Vectors / Q000469:parasitology*; D051379:Mice; D008815:Mice, Inbred Strains; D012756:Sheep; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity