title: Tissue culture of human choroidal melanoma cells obtained by fine-needle aspiration biopsy.
abstract: The optimal treatment of choroidal melanoma is controversial. Many patients are now being treated with nonenucleation therapies. Fine-needle aspiration biopsy of intraocular tumors may well prove to be safe; we used a needle biopsy technique on three freshly enucleated globes and established choroidal melanoma cell lines in culture from these specimens. This report describes our needle biopsy and tissue culture techniques.
mesh_terms: D001707:Biopsy, Needle; D002830:Choroid Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D046508:Culture Techniques; D006652:Histological Techniques; D006801:Humans; D008545:Melanoma / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron