Modulation by concanavalin A of the hormonal response in DNA-synthetic and transcriptional activity of a mouse mammary adenocarcinoma culture.
The tissue responds in culture differently to each of the tested hormones. Insulin, but not prolactin or hydrocortisone, was mitogenic to the culture, but did not alter the overall transcriptional activity pattern displayed by the insulin-free cultures. Prolactin stimulated transcriptional activity above control levels between days 2 and 6 of culture, but hydrocortisone was inhibitory to both DNA-synthetic and transcriptional activity of the culture. Upon initial 24-hr treatment with 50 micrograms/ml monovalent concanavalin A, the tissue altered its hormonal sensitivity in the examined activities, but the presence of any one of the hormones in the culture medium abolished the individual effect of concanavalin A. With hydrocortisone, however, the individual effects of the lectin and the hormone interacted in cultures treated with both compounds, suggesting a type of intracellular communication with the membrane, operative in this mammary cell line, that may involve channels of biochemical changes energized by a hydrocortisone stimulus.
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D046508:Culture Techniques; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D006728:Hormones / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011388:Prolactin / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D004273:DNA, Neoplasm; D006728:Hormones; D007328:Insulin; D012333:RNA, Messenger; D003208:Concanavalin A; D011388:Prolactin; D006854:Hydrocortisone