title: Immunocytochemical demonstration of steroid hormones in the granulosa cells of the medaka, Oryzias latipes.
abstract: Immunocytochemical identification of steroid hormones was performed in granulosa cells isolated from large ovarian follicles of the medaka. The granulosa cells recovered from daily spawning females, 8.5 h after the onset of light, reacted with anti-progesterone and anti-17 alpha-hydroxyprogesterone antibody, while the granulosa cells treated 2.5 h after the onset of light did not. After 6 h stimulation with gonadotropin (PMS) in vitro, the isolated granulosa cells reacted with anti-progesterone antibody. The granulosa cells examined 8.5 h after the onset of light did not react with anti-estradiol or anti-estriol antibody. These results demonstrate that, in Oryzias latipes, progestins (progesterone and 17 alpha-hydroxyprogesterone) are synthesized in the granulosa cells which are directly stimulated by gonadotropin.
mesh_terms: D000818:Animals; D000906:Antibodies; D004958:Estradiol / Q000032:analysis*; D004964:Estriol / Q000032:analysis; D005260:Female; D005399:Fishes; D005455:Fluorescent Antibody Technique; D006107:Granulosa Cells / Q000166:cytology*; D009865:Oocytes / Q000166:cytology; D010777:Photochemistry; D011374:Progesterone / Q000032:analysis*
chemical_list: D000906:Antibodies; D011374:Progesterone; D004958:Estradiol; D004964:Estriol