Heterophil Forssman glycoprotein and adenovirus 12: transformed rat cell lines.
Immunobiological significance of Forssman (F) glycoprotein expressed on rat tumor cells derived from transfection of a fibroblast line with whole DNA (WY-3); EcoRI-C fragment, 0-16.5 map units (CY-1); and Accl-H fragment, 0-4.7 map units (HY-1) of adenovirus 12 was investigated. Culturing of F-positive WY-3 and CY-1 cells, but not F-negative HY-1 cells, with monoclonal rat F-antibody resulted in the blocking of their cell-cell adhesion and attachment to plastic surface and inhibition of their growth. Immunization of WY-3 or CY-1 tumor-bearing F344 rats with sheep red blood cells or purified F-antigen in adjuvants brought regression of the tumor in 13 of 19 rats. No such antitumor effect was observed in F-negative HY-1 tumor-bearing rats upon immunization with the F-antigens. Results of this study indicated that the membrane glycoprotein with F-epitope may play a role in lodgement of the tumor cells in vitro and serve as an in vivo target of specific immune effectors.
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D015478:Antigens, Heterophile / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral; D005455:Fluorescent Antibody Technique; D005577:Forssman Antigen / Q000032:analysis / Q000276:immunology*; D006651:Histocytochemistry; D007114:Immunization; D007124:Immunoenzyme Techniques; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344
D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D000954:Antigens, Surface; D004279:DNA, Viral; D005577:Forssman Antigen