Immunolocalization of a spectrin-like protein (fodrin) in pancreatic acinar cells.
A spectrin-like protein (fodrin) was localized in porcine pancreas using an immunoperoxidase procedure with antibodies raised against erythrocyte spectrin. Fodrin was primarily associated with the cell plasma membrane although some was also detectable in the cytoplasm of the acinar cells. The membrane labelling of the acinar cells was uneven such that the lateral and basal membranes were strongly labelled by anti-spectrin antibodies whereas the apical membranes were poorly labelled. The implications of the results to secretion and to the occurrence of specific membrane domains are discussed.
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D002462:Cell Membrane / Q000032:analysis; D007124:Immunoenzyme Techniques; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis* / Q000276:immunology; D010179:Pancreas / Q000032:analysis / Q000166:cytology*; D013049:Spectrin / Q000276:immunology; D013552:Swine
D002352:Carrier Proteins; D008840:Microfilament Proteins; D013049:Spectrin