ELISA detection of influenza haemagglutinin depends on its molecular environment.
Two ELISA techniques (indirect and sandwich) were used for detection of influenza HA in three HA-containing specimens: whole influenza virus, HA + NA, isolated HA. Adsorptive and antigenic properties of the HA were found to depend on its physicochemical state and molecular environment.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006389:Hemagglutinins, Viral / Q000032:analysis*; D007163:Immunosorbent Techniques; D009439:Neuraminidase / Q000276:immunology; D009975:Orthomyxoviridae / Q000276:immunology*; D011817:Rabbits
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D009439:Neuraminidase