title: The assessment of the chemotherapeutic effects of vinca alkaloids by immunofluorescence and flow cytometry.
abstract: The effects of the vinca alkaloids on the rates of DNA synthesis in the human pancreatic carcinoma line, MIA Pa Ca-2 have been studied by a new technique for measuring cell kinetics and DNA synthesis by flow cytometry and immunofluorescence. The method employs a monoclonal antibody that is highly specific for bromodeoxyuridine or iododeoxyuridine. The drugs vincristine and vindesine do not appear to have a direct effect on DNA synthesis rate across S phase, whereas DHAD, a compound that has been found previously to affect DNA synthesis, does appear by this technique to inhibit DNA synthesis at specific segments of S phase. That vincristine does not block cells in S phase by inhibiting DNA synthesis is borne out of the observation that cells blocked in S or G2 + M can still incorporate BrdUrd at a high rate in these phases of the cell cycle.
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002460:Cell Line; D003201:Computers; D004261:DNA Replication; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007399:Interphase / Q000187:drug effects; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D013997:Time Factors; D014748:Vinca Alkaloids / Q000627:therapeutic use*; D014750:Vincristine / Q000627:therapeutic use
chemical_list: D014748:Vinca Alkaloids; D014750:Vincristine; D001973:Bromodeoxyuridine