title: Control of mammary gland fibroblast growth by insulin, growth hormone and prolactin.
abstract: Fibroblasts isolated from guinea pig mammary glands were cultured in 96 well culture plates in the presence of various concentrations of insulin, growth hormone and prolactin. Insulin (30 micrograms/ml increased uptake of tritiated thymidine by 30%. Higher concentrations of insulin did not result in any further increase in thymidine uptake. Growth hormone alone did not alter thymidine uptake in concentrations of 0 to 250 ng/ml. 300 ng/ml gave thymidine uptake of 136% of controls. In the presence of 20 g/ml insulin, growth hormone (250 ng/ml) increased thymidine uptake to approximately double that of controls. Prolactin alone (300 ng/ml decreased thymidine uptake by 19%. Insulin increased thymidine uptake, but the negative effect of prolactin was still evident above 150 ng/ml.
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D006168:Guinea Pigs; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000187:drug effects; D011388:Prolactin / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D007328:Insulin; D014316:Tritium; D011388:Prolactin; D013006:Growth Hormone; D013936:Thymidine