Specific small nuclear RNAs are associated with yeast spliceosomes.
Two different methods have been devised for the analysis and purification of spliceosomes formed in a yeast in vitro splicing system. The first method relies on the electrophoretic separation of ribonucleoprotein particles in composite acrylamide-agarose gels. A large fraction of added substrate is located in spliceosomes, the formation of which can be shown to be dependent on the presence of both a yeast 5' splice junction and a TACTAAC box on the RNA substrate. The second method relies on oligo(dT)-cellulose chromatography of spliceosomes formed with a polyadenylated substrate. Purification of spliceosomes by either method indicates that at least three small nuclear RNAs, approximately 160, 185, and 215 nucleotides in length, are specifically associated with yeast spliceosomes.
D002845:Chromatography; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis; D012326:RNA Splicing; D012331:RNA, Fungal / Q000032:analysis*; D012342:RNA, Small Nuclear / Q000032:analysis*; D012261:Ribonucleoproteins / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000235:genetics*
D005656:Fungal Proteins; D012331:RNA, Fungal; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins