Two species of dermatan sulfate proteoglycans with different molecular sizes from newborn calf skin.
Two species of dermatan sulfate-proteoglycans (DS-PGs) were isolated from calf skin. The first species, PDS-H (high-molecular-weight proteodermatan sulfate), contains the core protein with a molecular weight of either about 55,000 or 53,000. Both the core proteins are capable of binding to concanavalin A (Con A). The second species, PGs-L (low-molecular-weight proteoglycan containing dermatan sulfate and/or chondroitin sulfate), contains a core protein of Mr = 20,000 that did not bind to Con A. Tryptic peptide mappings revealed that Mr = 55,000 core protein and Mr = 53,000 core protein were of the same origin. However, the tryptic peptides and the amino acid composition of PGs-L core protein were completely different from those of PDS-H core proteins. The polyclonal antibodies against Mr = 55,000 core protein reacted with both the core proteins of Mr = 55,000 and Mr = 53,000 but not with the core protein from PGs-L. The DS was found to be the only glycosaminoglycan component of PDS-H. That is, the glycosaminoglycan from PDS-H was composed of 46% iduronosylhexosamine units and 54% glucuronosylhexosamine units, while the glycosaminoglycan of PGs-L was composed of 30% iduronosylhexosamine units and 70% glucuronosylhexosamine units.
D000818:Animals; D000831:Animals, Newborn; D001216:Asparagine / Q000032:analysis; D002417:Cattle; D002807:Chondroitin / Q000031:analogs & derivatives*; D003871:Dermatan Sulfate / Q000031:analogs & derivatives* / Q000145:classification / Q000302:isolation & purification; D006025:Glycosaminoglycans / Q000032:analysis; D007158:Immunologic Techniques; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D011509:Proteoglycans / Q000145:classification / Q000302:isolation & purification*; D012867:Skin / Q000032:analysis*; D014357:Trypsin
D006025:Glycosaminoglycans; D009844:Oligosaccharides; D011509:Proteoglycans; C028774:proteodermatan sulfate; D003871:Dermatan Sulfate; D001216:Asparagine; D002807:Chondroitin; D014357:Trypsin