Immunohistochemical demonstration of histamine N-methyltransferase-like structures in rat kidney.
Histamine N-methyltransferase (S-adenosylmethionine: histamine N-methyltransferase, E.C. 2.1.1.8) was purified to homogeneity from rat kidney, and antibody was raised against it in guinea pigs. The antibody immunoprecipitated histamine N-methyltransferase. Immunofluorescent histochemical studies with anti-histamine N-methyltransferase antibody as the first antibody and goat anti-guinea pig IgG conjugated with fluorescein isothiocyanate as the second, showed the presence of immunoreactive structures in the proximal tubules of rat kidney. The brain showed no immunoreaction with the antibody.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D006637:Histamine N-Methyltransferase / Q000032:analysis* / Q000276:immunology; D006651:Histocytochemistry / Q000379:methods; D007668:Kidney / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008780:Methyltransferases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D008780:Methyltransferases; D006637:Histamine N-Methyltransferase