Killer toxin from Hansenula mrakii selectively inhibits cell wall synthesis in a sensitive yeast.
Hansenula mrakii secretes extracellularly a killer toxin which kills sensitive Saccharomyces cerevisiae. In protoplasts of this yeast, the killer toxin selectively inhibited the synthesis of alkali-insoluble acid-insoluble polysaccharides consisting mainly of beta-glucan, but did not inhibit either the synthesis of other cell wall polysaccharides, such as mannan, chitin and alkali-insoluble acid-soluble polysaccharides, or the synthesis of protein. Consistent with these results, the toxin was inhibitory to the beta-(1,3)-glucan synthetase activity of a cell-free extract from sensitive S. cerevisiae.
D002473:Cell Wall / Q000502:physiology; D002686:Chitin / Q000096:biosynthesis; D005656:Fungal Proteins / Q000096:biosynthesis; D005936:Glucans / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D055646:Killer Factors, Yeast; D007700:Kinetics; D008351:Mannans / Q000096:biosynthesis; D009183:Mycotoxins / Q000494:pharmacology*; D010843:Pichia; D011134:Polysaccharides / Q000096:biosynthesis; D011523:Protoplasts / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales
D005656:Fungal Proteins; D005936:Glucans; D055646:Killer Factors, Yeast; D008351:Mannans; D009183:Mycotoxins; D011134:Polysaccharides; D029701:Saccharomyces cerevisiae Proteins; D002686:Chitin; D005947:Glucose