Genetic studies on the beta subunit of Escherichia coli RNA polymerase. IX. The role of the carboxy-terminus in enzyme assembly.
The assembly of RNA polymerase was studied in Escherichia coli mutants encoding large N-terminal amber fragments of the beta subunit. Whereas the removal of up to 20% of the carboxy-terminus does not prevent the formation of premature core enzyme, the amber fragments seem to interfere with holoenzyme production. These studies permit, therefore, the localization of a region on the beta polypeptide involved in sigma binding.
D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005838:Genotype; D046911:Macromolecular Substances; D008970:Molecular Weight; D009154:Mutation; D011499:Protein Processing, Post-Translational; D013045:Species Specificity
D046911:Macromolecular Substances; D012321:DNA-Directed RNA Polymerases