Identification and characterization of a calcium-binding protein in the mouse chorioallantoic placenta.
Mouse chorioallantoic placenta contains a specific calcium-binding protein (MCaBP). A procedure involving gel filtration and ion-exchange chromatography was developed to purify the MCaBP. The MCaBP activity increased as a function of embryonic gestation and was highly specific for Ca2+. The MCaBP is a monomeric protein of Mr 57000, with pI 4.7. Specific antibodies were prepared against the MCaBP and were used to localize the MCaBP to syncytiotrophoblasts of the chorionic villi of mouse chorioallantoic placenta. These properties suggest that the MCaBP may be involved in transplacental calcium transport.
D000818:Animals; D017317:Annexin A6; D002135:Calcium-Binding Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D002412:Cations / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy
D017317:Annexin A6; D002135:Calcium-Binding Proteins; D002412:Cations