Identification of anti-Lea by platelet complement fixation.
Two anti-Lea sera which were able to detect Lea antigen on platelets were identified in a screening for anti-platelet antibodies by means of a platelet complement fixation test. These two antisera hemolyzed erythrocytes without enzyme treatment. The anti-Lea activity could be completely absorbed by red cells, platelets and lymphocytes of Le(a+b-) donors but not by cells from Le(a-b+) or Le(a-b-) donors. The antibody activity against red cells was eliminated by treatment of the antisera with dithiothreitol, thereby suggesting that the activity resided in the IgM class of immunoglobulins. As the anti-Lea was more reactive at 37 degrees C than at room temperature against both red cells and platelets, we suggest that transfusion of platelets of Lea-negative donors should be considered for patients with this type of anti-Lea.
D001792:Blood Platelets / Q000276:immunology*; D003168:Complement Fixation Tests; D006461:Hemolysis; D006801:Humans; D007106:Immune Sera; D007201:Indicator Dilution Techniques; D007518:Isoantibodies / Q000032:analysis*; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D013696:Temperature
D007106:Immune Sera; D007518:Isoantibodies; D007983:Lewis Blood Group Antigens