[Chemical mutagenesis and the use of indirect enzymatic criteria for the selection of virulent clones of Bacillus thuringiensis].
The effect of the mutagene nitrosoguanidine (N-methyl-N'-nitro-N-nitrosoguanidine) on the growth of Bacillus thuringiensis subsp. galleriae st. 69/6 was being studied. It depends on the physiological state of the cells, dose of the mutagene, pH of the culture medium and exposition. Nitrosoguanidine was found to have the maximum mutagenic effect on the vegetative cells at pH 6.2 and on the spores at pH 5.6.
D001413:Bacillus thuringiensis / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D001431:Bacteriological Techniques; D009154:Mutation; D009604:Nitrosoguanidines / Q000633:toxicity; D010405:Penicillinase / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis; D012641:Selection, Genetic; D014774:Virulence
D009604:Nitrosoguanidines; D010447:Peptide Hydrolases; D010405:Penicillinase
Khimicheskiĭ mutagenez i ispol'zovanie kosvennykh énzimaticheskikh kriteriev dlia otbora virulentnykh klonov kul'tury Bacillus thuringiensis.