title: Neuronal differentiation of cloned human teratoma cells in response to retinoic acid in vitro.
abstract: We have analysed immunocytochemically the differentiation in vitro of clones of the human teratoma cell line Tera-2. The proliferating stem cells expressed Thy-1 and N-CAM/D2-CAM antigens. On treatment with 5 X 10(-5) M retinoic acid in either monolayer or aggregate cultures they began to express receptors for tetanus toxin and McAb A2B5. Three weeks after initiating retinoic acid treatment, the cultures contained a variety of cell morphologies, including about 3% of cells with a neuron-like morphology. These cells were reactive with tetanus toxin, McAb A2B5, and antibodies against Thy-1 and N-CAM/D2-CAM. They also expressed 55 000 and 210 000 Da neurofilament subunits.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D015815:Cell Adhesion Molecules; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009474:Neurons / Q000276:immunology / Q000378:metabolism; D013724:Teratoma / Q000276:immunology / Q000503:physiopathology*; D013744:Tetanus Toxin / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D013744:Tetanus Toxin; D014212:Tretinoin