Phosphoethanolamine- and fructose 1,6-diphosphate-induced calcium uptake in bone formed in vitro.
The purpose of this investigation was to determine whether biologically available organic phosphates other than beta-glycerophosphate were capable of inducing mineralization of bone in vitro. The chick periosteal osteogenesis model was used to demonstrate that endogenously available organic phosphates, fructose 1,6-diphosphate (F1,6-D) and phosphoethanolamine (PEA) induce calcium accumulation in bone formed in vitro. Alkaline phosphatase activity was inhibited in a dose-dependent manner by PEA, and the sodium salt of F1,6-D. There was an inverse correlation between alkaline phosphatase activity and organic phosphate-mediated mineralization. The data demonstrate that certain biologically available organic phosphates can induce mineralization and modulate bone metabolism in vitro.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000493:pharmacokinetics*; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D004983:Ethanolamines / Q000494:pharmacology*; D005635:Fructosediphosphates / Q000494:pharmacology*; D006598:Hexosediphosphates / Q000494:pharmacology*; D066298:In Vitro Techniques
D004983:Ethanolamines; D005635:Fructosediphosphates; D006598:Hexosediphosphates; C005448:phosphorylethanolamine; D000469:Alkaline Phosphatase; C029063:fructose-1,6-diphosphate; D002118:Calcium