Reduction of total hemolytic complement activity with Naja haje cobra venom factor does not prevent endotoxin-induced lung injury in sheep.
We studied the effects of reducing total hemolytic complement activity with Naja haje cobra venom factor on the lung injury caused by intravenously infused endotoxin in 5 unanesthetized sheep with lung lymph fistulas. In normal sheep, infusions of lipopolysaccharide W from Escherichia coli (1.0 micrograms/kg) intravenously over 30 min caused increases in protein-rich lung lymph flow as well as the appearance in plasma and lung lymph of complement (C5)-derived chemotactic activity for polymorphonuclear leukocytes. Reduction of total hemolytic complement activity by treatment with Naja haje cobra venom factor (12 to 17 U/kg intraperitoneally) did not prevent the lung injury caused by endotoxin and also did not prevent the appearance in plasma and lung lymph of chemotactic activity. We conclude that although complement appears to be activated following intravenously infused endotoxin in sheep, a completely intact complement system is not necessary for endotoxin-induced lung injury.
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D002634:Chemotaxis, Leukocyte; D003165:Complement System Proteins / Q000502:physiology*; D004546:Elapid Venoms / Q000494:pharmacology*; D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli; D006461:Hemolysis; D008171:Lung Diseases / Q000139:chemically induced*; D008196:Lymph / Q000378:metabolism; D008208:Lymphatic System / Q000502:physiology; D011652:Pulmonary Circulation; D012756:Sheep; D014655:Vascular Resistance / Q000187:drug effects
D001427:Bacterial Toxins; D004546:Elapid Venoms; D004731:Endotoxins; C016310:cobra venom factor; D003165:Complement System Proteins