title: Escherichia coli xthA mutant is not hypersensitive to ascorbic acid/copper treatment--an H2O2 generating reaction.
abstract: Ascorbate (vitamin C) in the presence of copper yields H2O2, which seems to be responsible for its toxic effects in bacteria. However, we found that the Escherichia coli xthA mutant strain, which is hypersensitive to H2O2, has almost the same sensitivity as the wild-type strain to ascorbate and copper treatment. Our results suggest that the DNA damage induced in E. coli by H2O2 generated in oxidized ascorbate solutions is different from that induced by direct H2O2 treatment.
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D003300:Copper / Q000494:pharmacology*; D004260:DNA Repair; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000502:physiology; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010084:Oxidation-Reduction
chemical_list: D001426:Bacterial Proteins; D005609:Free Radicals; D003300:Copper; D006861:Hydrogen Peroxide; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D001205:Ascorbic Acid