Determination of adriamycin and its fluorescent metabolites in human plasma by HPLC.
We describe a method for measuring adriamycin and its metabolites, adriamycinol and adriamycinone in plasma, using reversed phase HPLC and fluorescence detection. The lower limit of detection is approximately 1 ng/mL for each compound. An extraction technique for serum is described which is capable of an almost equal recovery (greater than 93%) of adriamycin and metabolites without interference from endogenous components of plasma and from other common drugs. Within-day and day to day coefficients of variation are estimated.
D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000097:blood*; D006801:Humans; D009279:Naphthacenes / Q000097:blood; D013050:Spectrometry, Fluorescence
D009279:Naphthacenes; C010012:adriamycin aglycone; D004317:Doxorubicin; C010013:adriamycinol