title: Dependency of B cells on the presence of adherent cells, or factors derived from them, for the production of autoantibodies in vitro in the absence of cell division.
abstract: Peritoneal cells from untreated mice secrete autoantibodies after 3-4 days of in vitro culture, although the cells do not divide. Here, peritoneal cells enriched for B cells to contain 95% surface Ig-bearing cells, did not secrete autoantibodies after 3 days of in vitro culture unless plastic-adherent cells derived from the peritoneal cavity were cultured with the B cells. Cell-free media, taken from peritoneal adherent cells that had been cultured for 3 days in vitro, when added a final concentration of 50% in fresh culture medium to purified B cells, substituted for the presence of accessory cells. In contrast to cultures of unfractionated peritoneal cells, little increase in precursor frequency was detected when enriched B cells were cultured in the presence of LPS/DXS. However, the addition of adherent cells, supernatants derived from adherent cells, or cytokines produced by a T-cell hybrid EL4, resulted in an increased precursor frequency when LPS/DXS was added to the culture medium. Three macrophage cell lines, P388-D1, J774, and PU-5-IR, when added to purified B cells. augmented the autoantibody precursor frequency detected in vitro. This is strong evidence that potentially autoreactive B cells require one or more types of accessory cells in order to differentiate into autoantibody secretors during culture in vitro. Further, the results provide indirect evidence that interleukin 1 may be a crucial molecule in the differentiation of B cells.
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology*; D001688:Biological Products / Q000502:physiology; D002448:Cell Adhesion; D002453:Cell Cycle; D002478:Cells, Cultured; D016207:Cytokines; D005260:Female; D008264:Macrophages / Q000502:physiology; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology
chemical_list: D001323:Autoantibodies; D001688:Biological Products; D016207:Cytokines