Studies on the biological activity of an insulin-stimulating peptide from a tryptic digest of bovine serum albumin.
A two-chain polypeptide, which corresponds to amino acid residues 115-143 and 144-184(185) of bovine serum albumin, connected to each other by a disulfide bridge, potentiated the effects of insulin on glucose transport and glucose metabolism in isolated rat adipocytes. Although the peptide alone had little activity, it shifted the concentration-response curves of insulin-stimulated D-[1-14C]glucose oxidation, 2-deoxyglucose transport, and lipid synthesis from D-[U-14C]glucose to lower insulin concentrations. It also increased the maximal responses of these parameters to insulin. However, it did not affect insulin binding to adipocytes. The peptide protected insulin considerably from degradation, but this effect alone cannot account for its effect in increasing the maximal responses to the hormone, and even when degradation of a submaximal concentration of insulin was suppressed by bacitracin, the peptide still had an enhancing effect. These results suggest not only that the peptide influences a step distal to receptor-mediated insulin binding but also that inhibition of insulin degradation alone cannot explain its total effect. The peptide lost its insulin-stimulating activity completely when it was further digested with V8 or lysine-specific endopeptidase, or when it was reduced and then carboxamidomethylated or oxidized with performic acid. Similar active tryptic fragments were obtained from human and rat albumins. Insulin-stimulating peptides should be useful in studies on the mechanisms of insulin action including both the sensitivities and responsiveness of target cells to the hormone.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001414:Bacitracin / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D005227:Fatty Acids / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism
D005227:Fatty Acids; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D010455:Peptides; C047279:insulin-stimulating peptide, human; D001414:Bacitracin; D012710:Serum Albumin, Bovine; D014357:Trypsin; D005947:Glucose