Ganglioside GM1 localization in cholinergic neurons visualized by use of a double avidin-biotin complex system.
Localization of ganglioside GM1 in cholinergic neurons from the septal area of a primary culture newborn rat brain was studied with a double avidin-biotin complex system. Cholinergic neurons were identified by double immunolabeling techniques that use choline acetyltransferase (ChAT) and neurofilament (NF) protein-antibodies. ChAT-positive neurons also were stained for ganglioside GM1 by using an avidin-biotin complex technique.
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies; D001360:Avidin; D001710:Biotin; D001921:Brain / Q000166:cytology* / Q000201:enzymology; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside / Q000032:analysis*; D007382:Intermediate Filaments / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D051381:Rats
D000906:Antibodies; D001360:Avidin; D005677:G(M1) Ganglioside; D001710:Biotin; D002795:Choline O-Acetyltransferase