Multiple DNA-dependent RNA polymerases in Trypanosoma cruzi.
DNA-dependant RNA polymerase solubilized from cultured Trypanosoma cruzi epimastigotes was chromatographed on A-25 Sephadex and gave a single peak of activity. Subsequent phosphocellulose chromatography revealed two peaks of RNA polymerase activity. These peaks have different sensitivities to the toxin alpha-amanitin. The first peak is 50% inhibited by 17.8 micrograms ml-1 amanitin while the second peak is 50% inhibited by an amanitin concentration of 44.6 micrograms ml-1. The activity of both peaks is blocked by actinomycin D, but is unaffected by rifampicin. Each peak is stimulated by Mn2+, and is optimal with single stranded DNA as a template.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002852:Chromatography, Ion Exchange; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics
D000546:Amanitins; D003609:Dactinomycin; D008345:Manganese; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin