Localization of the streptococcal C5a peptidase to the surface of group A streptococci.
Immunofluorescent staining was used to determine that the streptococcal C5a peptidase (SCP) exists as a cell surface antigen on group A streptococci. The ability of hyperimmune serum to neutralize cell-associated SCP activity provided further evidence for the location of SCP. Quantification of SCP during growth in vitro by indirect enzyme-linked immunosorbent assay showed that approximately 90% of the measurable antigen is cell bound.
D000954:Antigens, Surface / Q000032:analysis; D003182:Complement C5 / Q000378:metabolism*; D015936:Complement C5a; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D010447:Peptide Hydrolases / Q000032:analysis* / Q000276:immunology; D013297:Streptococcus pyogenes / Q000201:enzymology*
D000954:Antigens, Surface; D003182:Complement C5; D007106:Immune Sera; D015936:Complement C5a; D010447:Peptide Hydrolases