Characteristics of fresh isolates of wild measles virus.
Characteristics of fresh isolates of wild measles virus were studied by immunofluorescence using monoclonal antibodies, SDS-polyacrylamide gel electrophoresis, and electron microscopy. By the immunofluorescence test, hemagglutinin, nucleocapsid-associated phosphorylated, and fusion proteins were found only in the cytoplasm of cells infected with the virus at a low passage level. Nucleocapsid (NP) and membrane (M) proteins were found in both the cytoplasm and nuclei. Since the M protein is present only in the cytoplasm of cells infected with the laboratory strain of measles virus, this intranuclear M protein may be specific to the wild virus. At an increased passage level, the intranuclear M protein became undetectable, but the NP protein was still present in the nuclei. SDS-polyacrylamide and electron microscopy showed that wild measles viruses were shown to have characteristics similar to those of laboratory strains.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002213:Capsid / Q000032:analysis*; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000032:analysis; D005347:Fibroblasts / Q000382:microbiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006389:Hemagglutinins, Viral / Q000032:analysis*; D006801:Humans; D007668:Kidney; D008459:Measles virus / Q000032:analysis* / Q000276:immunology; D014758:Viral Core Proteins / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000032:analysis*; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D006389:Hemagglutinins, Viral; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins