Participation of the dnaK and dnaJ gene products in phosphorylation of glutaminyl-tRNA synthetase and threonyl-tRNA synthetase of Escherichia coli K-12.
The heat shock proteins DnaK and DnaJ of Escherichia coli participate in phosphorylation of both glutaminyl-tRNA synthetase and threonyl-tRNA synthetase. When cellular proteins extracted from the dnaK7(Ts) and dnaJ259(Ts) mutant cells labeled with 32Pi at 42 degrees C were analyzed by two-dimensional gel electrophoresis, no phosphorylation of these proteins was observed when they were compared with those from wild-type cells.
D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006360:Heat-Shock Proteins / Q000378:metabolism*; D009154:Mutation; D010766:Phosphorylation; D010769:Phosphothreonine / Q000032:analysis; D013696:Temperature; D013914:Threonine-tRNA Ligase / Q000032:analysis / Q000378:metabolism*
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D010769:Phosphothreonine; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase; D013914:Threonine-tRNA Ligase