Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg).
Conjugates of goat anti-HBs IgG and horseradish peroxidase (HRP) prepared by two different methods, one using NaIO4 and the other SPDP, were compared. Anti-HBs antibodies obtained from goat, rabbit and guinea-pig were tested as capture serum. The ELISA showed a sensitivity similar to RIA and a level of antigen captation ranging from 4.37 to 8.75 nanograms/ml was obtained when rabbit or guinea-pig captures were used combined with both NaIO4 or SPDP conjugates.
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D006168:Guinea Pigs; D006510:Hepatitis B Antibodies / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D007202:Indicators and Reagents; D011817:Rabbits
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007106:Immune Sera; D007202:Indicators and Reagents