title: Comparison of the MicroScan system with the API Staph-Ident system for species identification of coagulase-negative staphylococci.
abstract: To evaluate the accuracy of the MicroScan System (American Hospital Supply Corp., Sacramento, Calif.) for identification of coagulase-negative staphylococci, we tested 175 clinical isolates of coagulase-negative staphylococci. The results obtained by the MicroScan system were compared with those of the API Staph-Ident system (Analytab Products, Plainview, N.Y.). Forty-three discrepancies between the two systems were resolved by the conventional method of Kloos and Schleifer (W.E. Kloos and K.H. Schleifer, J. Clin. Microbiol. 1:82-88, 1975). The MicroScan and the Staph-Ident systems correctly identified 146 (86.4%) and 154 (88%) of 175 strains, respectively. The API system failed to identify phosphatase-negative Staphylococcus epidermidis. The MicroScan system demonstrated the greatest accuracy in the identification of S. epidermidis and S. saprophyticus, whereas lesser accuracy was achieved with S. hominis, S. warneri, and S. sciuri.
mesh_terms: D001431:Bacteriological Techniques; D003030:Coagulase / Q000378:metabolism; D003201:Computers; D005069:Evaluation Studies as Topic; D006801:Humans; D011933:Reagent Kits, Diagnostic; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000145:classification* / Q000201:enzymology / Q000378:metabolism
chemical_list: D003030:Coagulase; D011933:Reagent Kits, Diagnostic