Characterization of rabbit uterine estrogen receptor proteins by radioiodination and partial peptide mapping.
Two estrogen binding proteins (Mr = 50,000 and 65,000) were purified from rabbit uterine cytosol using an improved procedure for affinity chromatography on diethylstilbestrol-agarose. The estrogen receptors were radioiodinated while adsorbed to the resin using the lactoperoxidase or Bolton-Hunter techniques. After elution, the labeled receptors were utilized for peptide mapping studies and investigations of receptor function. Partial peptide mapping revealed strong homology between the Mr 50,000 and 65,000 proteins suggesting common structural features. Estrogen receptors labeled by the lactoperoxidase procedure were rendered unable to bind immobilized heparin or hormone; in contrast, the Bolton-Hunter labeling technique yields proteins that retain both their ability to bind hormone and to absorb on heparin-agarose. The development of these iodination methodologies appears useful for the investigation of both the structure and functional properties of the receptor proteins.
D000818:Animals; D002846:Chromatography, Affinity; D004054:Diethylstilbestrol / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D007784:Lactoperoxidase / Q000378:metabolism; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis*; D011817:Rabbits; D011960:Receptors, Estrogen / Q000302:isolation & purification / Q000378:metabolism*; D012697:Serine Endopeptidases; D013388:Succinimides; D014158:Transcription, Genetic; D014357:Trypsin / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
D010446:Peptide Fragments; D011960:Receptors, Estrogen; D013388:Succinimides; C022581:Bolton-Hunter reagent; D004054:Diethylstilbestrol; D007784:Lactoperoxidase; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin