Impaired spermatogenesis in the finch hybrid L. castaneothorax by L. punctulata: transmission electron microscopy and genetic analysis.
As part of a project to determine whether there is any correlation between the form of hybrid sterility and the genetic relatedness of the parental species, we studied a male intrageneric hybrid between two finch species (Lonchura castaneothorax X L. punctulata), and compared the ultrastructural basis of hybrid sterility in this species with that reported by Swan [1985] for an intergeneric bird hybrid. In the latter study the sterility appeared to have an autoimmune basis, due to lack of Sertoli-Sertoli tight junctions. In the hybrid examined in the present study, lanthanum tracing showed that the junctions were tight. There was no testicular immune reaction; the parental species were almost identical in chromosomal constitution, having only a small inversion difference on chromosome 5, and only two structural protein differences could be detected through examination of the variation at 38 protein loci. Nevertheless, the hybrid appeared sterile and had the following ultrastructural testicular features. Intercellular bridges where present were usually abnormal in structure; centrioles in a centriole pair were arranged in parallel. Many spermatocytes and spermatids degenerated and were phagocytosed by Sertoli cells. Some spermatids progressed to mature testicular spermatozoa in sperm bundles, but commonly had multiple (2-4) axonemes or disrupted doublets and accessory fibers. The multiple axonemes present in most spermatids inserted separately into the base of the nucleus and the multiple centrioles were capable of organizing separate neck structures. We conclude that these cytological abnormalities were caused by genic effects and discuss why they appeared to be restricted to the germ line.
D000818:Animals; D001717:Birds / Q000235:genetics*; D003433:Crosses, Genetic; D005260:Female; D006824:Hybridization, Genetic; D007621:Karyotyping; D008297:Male; D008854:Microscopy, Electron; D013091:Spermatogenesis; D013094:Spermatozoa / Q000002:abnormalities* / Q000648:ultrastructure; D013737:Testis / Q000648:ultrastructure*