title: Influence of the incubation of cells with zinc and lithium ions on GVH reactivity of cells and on their ability to form haemopoietic colonies.
abstract: Mouse spleen and bone marrow cells were incubated for 2 h with ZnCl2 and Li2SO4 at different concentrations and tested for the ability to evoke the graft-versus-host reactions (GVHR) and to form pluripotent haemopoietic colonies. ZnCl2 at concentrations 5 X 10(-6) to 5 X 10(-4) M inhibited the regional GVHR. At a concentration of 5 X 10(-6) ZnCl2 also inhibited the ability to elicit the systemic GVHR in irradiated mice. Haemopoiesis was stimulated in cells incubated with ZnCl2 at concentration 5 X 10(-6) M, it was inhibited after incubation of A/01a cells with 5 X 10(-4) M ZnCl2, whereas cells from non-inbred ICR mice were stimulated by the latter concentration of ZnCl2 for haemopoiesis. Li2SO4 inhibited the ability to induce the regional GVHR at concentrations of 5 X 10(-3) and 10(-2) M, had no effect on survival of mice during the systemic GVHR and exerted rather an inhibitory effect on haemopoiesis.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D005260:Female; D006087:Graft vs Host Reaction / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D066298:In Vitro Techniques; D008094:Lithium / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000187:drug effects / Q000637:transplantation; D014184:Transplantation, Homologous; D015032:Zinc / Q000494:pharmacology*
chemical_list: D008094:Lithium; D015032:Zinc