Trisomy 12 correlates with elevated expression of p21 ras in a human adenosquamous carcinoma of the lung.
Reported herein is the first case in which a specific chromosomal abnormality, namely trisomy 12, was observed in primary cultures of a human adenosquamous carcinoma of the lung. Because the c-K-ras had been shown to localize on chromosome 12, the relationship between trisomy and oncogene expression was examined in this tumor by concurrently assaying for the levels of ras oncogene protein product, p21, in both the tumor and adjacent normal tissues. An approximately tenfold increase in the level of p21 ras was observed in the tumor, compared with the adjacent normal tissue. The present finding suggests a possible relationship between chromosomal trisomy and oncogene activation.
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002881:Chromosomes, Human, Pair 12; D005260:Female; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D016283:Proto-Oncogene Proteins p21(ras); D014314:Trisomy; D014404:Tubulin / Q000032:analysis
D011518:Proto-Oncogene Proteins; D014404:Tubulin; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)