Measurement of penbutolol and 4-hydroxypenbutolol in plasma or serum by HPLC.
A simple HPLC method for penbutolol and 4-hydroxypenbutolol assay has been developed. Plasma or serum (200 microliters) is vortex-mixed (30 s) with Tris solution (2 M, pH 10.6) containing an internal standard (50 microliters) and methyl t-butyl ether (200 microliters). After centrifugation, the extract (100 microliters) is analysed using an unmodified silica column (250 x 5 mm ID) and iso-octane-methanol-methyl t-butyl ether (55:25:20) containing ammonium perchlorate (10 mM, pH 5.7) as eluent and with fluorescence detection. No interference has been encountered and the limit of accurate measurement for both compounds is 5 micrograms/l.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010394:Penbutolol / Q000031:analogs & derivatives / Q000097:blood*; D011412:Propanolamines / Q000097:blood*; D013050:Spectrometry, Fluorescence
D011412:Propanolamines; D010394:Penbutolol; C034270:4-hydroxypenbutolol