Designed transfer of specific immune responses with bone marrow transplantation.
Bone marrow transplant donors were immunized with tetanus/diphtheria toxoids 6-7 d before bone marrow donation to investigate the role of B cell subpopulations in reconstitution of humoral immunity. Lymphoblastoid B cells spontaneously producing IgG antitetanus and/or antidiphtheria toxoid were detected in the donor marrows at the time of transplantation. Recipients rapidly demonstrated 3-90-fold increases in serum IgG antitetanus and antidiphtheria toxoid levels. Antidiphtheria fragment A antibody in three donor/recipient pairs demonstrated spectrotypic identity indicating transfer of the donors' response. Reimmunization of three recipients 64-154 d after transplant revealed an IgG antibody response associated with reappearance of spontaneous antibody-producing B cells and an antidiphtheria fragment A response characteristics of the donor's immune response. These observations extend the understanding of the role of B cell subpopulations and provide a basis for specific modulation of immunity in the setting of bone marrow transplantation.
D000328:Adult; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D016026:Bone Marrow Transplantation; D004167:Diphtheria Toxin / Q000032:analysis; D004168:Diphtheria Toxoid / Q000276:immunology; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007938:Leukemia / Q000628:therapy; D010446:Peptide Fragments / Q000032:analysis; D013745:Tetanus Toxoid / Q000276:immunology
D004167:Diphtheria Toxin; D004168:Diphtheria Toxoid; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010446:Peptide Fragments; D013745:Tetanus Toxoid; C029795:diphtheria toxin fragment A