Specific purification of elongation factor 2 and isolation of its antibody.
Elongation factor 2 (EF-2) was purified from rat liver extracts by affinity chromatography using fragment A of diphtheria toxin as the ligand. Purified EF-2 has a molecular weight of 96,000 and isoelectric point of 6.6-6.8. The sequence of the nineteen N-terminal amino acid is Val-Asn-Phe-Thr-Val-Asp-Gln-Ile-Arg-Ala Ile-Met-Asp-Lys-Lys-Ala-Asn and the C-terminal amino acid is leucine. Purified rat EF-2 modified with ADP-ribose was injected into rabbits to prepare antibodies against EF-2. The anti-EF-2 antibodies can immunoprecipitate with EF-2 from various eukaryotic cells.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002846:Chromatography, Affinity / Q000379:methods; D003429:Cross Reactions; D004167:Diphtheria Toxin / Q000378:metabolism; D007526:Isoelectric Point; D008099:Liver / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000276:immunology
D000906:Antibodies; D004167:Diphtheria Toxin; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors