[Distribution of proteins and enzymes in 3 submitochondrial fractions isolated from the internal mitochondrial membrane of the rat liver].
Three fractions, A, B and C, were separated from rat liver inner mitochondrial membranes by sonication and centrifugation on a discontinuous sucrose gradient, and their distribution of enzyme activities, cytochromes and proteins was studied, in order to obtain data about the heterogeneous composition of the inner membrane. Fraction C was mitochondrial matrix. It contained about 90% of glutamate dehydrogenase activity, and its protein distribution in polyacrylamide gels was similar to that of mitochondrial matrix. Fraction B, with nearly 85% of enzyme activities and cytochrome contents of control inner membranes, would correspond to the crista membrane. Only 4% of cytochromes and enzyme activities of the inner membrane were found in fraction A, which would be the inner peripheral membrane, in contact with the outer membrane.
D000818:Animals; D003580:Cytochromes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D051381:Rats
D003580:Cytochromes; D004798:Enzymes; D008565:Membrane Proteins
Distribución de proteínas y enzimas en tres fracciones submitocondriales aisladas de la membrana interna mitocondrial de hígado de rata.