Inhibition of cellular immunity by products of Aspergillus fumigatus.
A fraction, FB, from extracts of Aspergillus fumigatus which previously was found to display a high degree of cellular hypersensitivity in skin tests in sensitized guinea-pigs but a low ability to affect transformation of lymphocytes in vitro, was studied. This fraction was found to be capable of inhibiting lymphocyte transformation induced in vitro by tuberculin and by T and B cell mitogens. The inhibitory properties of FB on lymphocyte activation were heat labile, destroyed by proteolysis, and could be reversed by washing 24 h after addition. Antibody to FB did not reduce inhibition of lymphocyte transformation. Fluorescence labeling studies showed a uniform and non-capping distribution of FB on lymphocyte surfaces which resulted in exclusion of attachment of fluorescein-labeled Concanavalin A to the cell surface.
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D006168:Guinea Pigs; D006358:Hot Temperature; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012882:Skin Tests; D014357:Trypsin / Q000494:pharmacology; D014373:Tuberculin / Q000276:immunology
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D014373:Tuberculin; D003208:Concanavalin A; D014357:Trypsin