title: Molybdenum cofactor: a compound in the in vitro activation of both nitrate reductase and trimethylamine-N-oxide reductase activities in Escherichia coli K12.
abstract: Nitrate reductase (nitrite: (acceptor) oxidoreductase, EC 1.7.99.4) and trimethylamine N-oxide reductase (NADH : trimethylamine-N-oxide oxidoreductase, EC 1.6.6.9) activities were reconstituted by incubation of the association factor FA (the putative product of the chlB gene) with the soluble extract of the chlB mutant grown anaerobically in the presence of trimethylamine N-oxide. When soluble extracts of the chlB mutant grown on 10 mM sodium tungstate, a molybdenum competitor, were used in complementation systems, no enzymatic reactivation was observed. Heated extracts of the parental strain 541 were shown to contain a thermoresistant molybdenum cofactor by their ability to reactivate NADPH-nitrate reductase activity in the nit1 mutant of Neurospora crassa. By complementation of parental strain heated extract with association factor FA and soluble extract of the chlB mutant grown in the presence of sodium tungstate, we were able to show for the first time that the molybdenum cofactor is an activator common to the in vitro reconstitution of both nitrate reductase and trimethylamine-N-oxide reductase activities.
mesh_terms: D003067:Coenzymes; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005816:Genetic Complementation Test; D008667:Metalloproteins; D008982:Molybdenum / Q000378:metabolism*; D000090006:Molybdenum Cofactors; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011621:Pteridines / Q000378:metabolism*
chemical_list: D003067:Coenzymes; D008667:Metalloproteins; D008982:Molybdenum; D000090006:Molybdenum Cofactors; D009247:NADH, NADPH Oxidoreductases; D050901:Nitrate Reductase; D009565:Nitrate Reductases; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011621:Pteridines; C022261:molybdopterin; C021567:methylamine dehydrogenase