Localization of cells containing LHRH-like mRNA in rat forebrain using in situ hybridization.
Using in situ hybridization, we localized cells in the rat forebrain which contain mRNA that hybridizes with a radiolabeled, synthetic oligodeoxyribonucleotide (59-mer) complementary to human LHRH mRNA in the region which includes the coding sequence for the decapeptide. These brain areas have been shown previously to contain immunoreactive LHRH cell bodies.
D000818:Animals; D004247:DNA; D004027:Diencephalon / Q000032:analysis* / Q000166:cytology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D007031:Hypothalamus / Q000032:analysis; D009693:Nucleic Acid Hybridization; D011301:Preoptic Area / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013687:Telencephalon / Q000032:analysis* / Q000166:cytology
D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; D004247:DNA