Antioxidant systems in tumour cells: the levels of antioxidant enzymes, ferritin, and total iron in a human hepatoma cell line.
The human hepatoma cell line Hep 3B, which has the hepatitis B virus genome, shows over 80% decrease of copper/zinc superoxide dismutase activity, over 90% decrease of manganese superoxide dismutase activity, over 70% decrease of catalase activity, absence of glutathione peroxidase and glutathione S-transferase activities, over 270-fold increase of ferritin content and 25-fold increase of total iron compared to normal autopsy liver. These conditions of low antioxidant enzyme activities and iron overload are those which support the accumulation of oxygen free-radicals and DNA damage commonly considered to be carcinogenic mechanisms.
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002461:Cell Line, Transformed; D003300:Copper / Q000378:metabolism; D005293:Ferritins / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D008345:Manganese; D003300:Copper; D005293:Ferritins; D007501:Iron; D010544:Peroxidases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D015032:Zinc