Evaluation of the endothelium of human donor corneas by induced dilation of intercellular spaces and trypan blue.
The endothelium of 30 pairs of human cadaver corneas was stained by trypan blue and the intercellular spaces were visualized by induced dilation prior to corneal culture. Trypan blue staining and induced dilation of intercellular spaces by 0.9% and 0.45% NaCl were found to be atraumatic. Only a fraction of damaged cells were stained by trypan blue. Endothelial cell losses in culture did not correlate with the number of trypan-blue stained cells, the post-mortem time, or donor age.
D000328:Adult; D000368:Aged; D002470:Cell Survival; D003315:Cornea / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D046508:Culture Techniques; D003584:Cytological Techniques / Q000009:adverse effects*; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005110:Extracellular Space / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D008875:Middle Aged; D014019:Tissue Donors; D014343:Trypan Blue / Q000009:adverse effects*
D014343:Trypan Blue