title: Passive immunization of mice against Klebsiella aerogenes.
abstract: Klebsiella vaccines were isolated by mild diafiltration techniques from culture filtrates of nine capsular types of K. aerogenes (K1, K2, K3, K15, K20, K35, K36, K44 & K63). The bacteria were grown in a chemically defined medium in standardized conditions in a fermenter. The vaccines had a molecular weight of more than 20 000, a carbohydrate content of 40-89%, a protein content of between less than 1 and 16% and small amounts (0.6-1.2%) of lipopolysaccharide. Antisera raised in rabbits to the nine klebsiella vaccines were standardized by passive haemagglutination, immunoglobulin G enzyme-linked immunosorbent assay and by autologous passive protection tests. Each rabbit antiserum when passively transferred to mice showed a variable capacity to passively protect mice against lethal infections by a panel of ten capsular types of K. aerogenes (K1-K10). Seven of the rabbit antisera protected mice against more than half of the challenge strains. A pool of six rabbit antisera (anti-K1, K2, K3, K20, K35 & K44) passively protected mice against lethal infections from strains of bacteria representing each of 77 capsular types of K. aerogenes.
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000032:analysis; D007710:Klebsiella Infections / Q000517:prevention & control*; D007711:Klebsiella pneumoniae; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D011817:Rabbits
chemical_list: D001428:Bacterial Vaccines; D007074:Immunoglobulin G