Novel antigens at the neuromuscular junction.
Three novel components of neuromuscular junctions have been identified by use of monoclonal antibodies (McAb) against glycoproteins obtained from a mouse neuroblastoma X human dorsal root ganglion cell hybrid line. Antigen distribution was assessed by fluorescent immunohistochemistry on frozen sections of human intercostal muscle counterstained with labeled alpha-bungarotoxin to identify neuromuscular junctions. Antigen SOS 6 stained exclusively in the neuromuscular junction, whereas antigens SOS 5 and SOS 13 were highly enriched in the junction but also stained extrasynaptic regions. These antigens can be distinguished from previously described components of the neuromuscular junction by their molecular weights, insensitivity to collagenase treatment, and solubility in 0.1% Triton X-100. Indirect evidence suggests that these species-specific antigens are located in the postsynaptic muscle membrane, but location in the junctional basal lamina or subsarcolemmal region cannot be excluded.
D000110:Acetylcholinesterase / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007366:Intercostal Muscles / Q000276:immunology; D008253:Macaca mulatta; D051379:Mice; D009132:Muscles / Q000276:immunology; D009469:Neuromuscular Junction / Q000276:immunology*; D017830:Octoxynol; D010215:Papio; D011092:Polyethylene Glycols; D051381:Rats
D000911:Antibodies, Monoclonal; D000941:Antigens; D011092:Polyethylene Glycols; D017830:Octoxynol; D000110:Acetylcholinesterase