The assay of chloramphenicol acetyltransferase activity by high performance liquid chromatography.
A high performance liquid chromatographic method is described for the assay of chloramphenicol acetyltransferase activity which detects the formation of both 3-acetoxy chloramphenicol and 1,3-diacetoxy chloramphenicol. In experiments performed at pH 6.8 only 3-acetoxy chloramphenicol was detected while in experiments performed at pH 7.8 both 3-acetoxy and 1,3-diacetoxy chloramphenicol were detected.
D000107:Acetylation; D000123:Acetyltransferases / Q000032:analysis*; D015500:Chloramphenicol O-Acetyltransferase; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase