Genetic complementation in Cryptococcus neoformans.
A complementation test was devised for the fungus Cryptococcus neoformans. Complementation was signalled by the growth of prototrophic heterokaryons generated in crosses of the type aB X Ab, where a and b represent any two of the genetic markers ilv1, cys1, cys2, and cys3. The cloned complementing heterokaryons formed characteristic hyphal colonies that contained both hyphae and yeast cells. The heterokaryon-derived yeasts were of three kinds: parental haploids, recombinant haploids, and diploids.
D003001:Cloning, Molecular; D003454:Cryptococcus / Q000235:genetics*; D003455:Cryptococcus neoformans / Q000235:genetics*; D005816:Genetic Complementation Test; D005838:Genotype; D006238:Haploidy; D011995:Recombination, Genetic