Platelet-associated IgG assay using flow cytometric analysis.
In this study we describe an immunofluorescent assay system to measure platelet-associated immunoglobulin G levels using flow cytometric analysis. This semi-quantitative system allows ready distinction of immune from non-immune related thrombocytopenias. It is simple to perform, highly reproducible and has the advantage of requiring a minimum concentration of 5000 platelets/microliter per assay sample.
D001327:Autoimmune Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D003240:Connective Tissue Diseases / Q000150:complications; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D010976:Platelet Count; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013921:Thrombocytopenia / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology
D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell