A method for the rapid detection of streptococcal group A antigen by specific monoclonal antibody.
In this study we evaluate the efficacy of a method for the rapid detection of group A streptococci employing a hybridoma mouse antibody specific against group A streptococcal carbohydrate. Using coagglutination technique we demonstrated an absolute specificity. The sensitivity was absolute if the preparation contained greater than or equal to 5 X 10(4) cocci. This test seems therefore a valid criterion for symptomatic patients with a larger number of group A streptococci in throat specimens, more sensitive than other methods, but not sensitive enough for carriers of few bacteria.
D000372:Agglutination Tests; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis*; D004925:Escherichia / Q000276:immunology; D007709:Klebsiella / Q000276:immunology; D008722:Methods; D009343:Neisseria / Q000276:immunology; D013210:Staphylococcus / Q000276:immunology; D013297:Streptococcus pyogenes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial