Oral immunization of mice with attenuated Salmonella enteritidis containing a recombinant plasmid which codes for production of the B subunit of heat-labile Escherichia coli enterotoxin.
We used Salmonella enteritidis serotype dublin strain SL1438, a nonreverting, aromatic-dependent, histidine-requiring mutant, as a recipient for a recombinant plasmid coding for production of the nontoxic B subunit of the heat-labile Escherichia coli enterotoxin. The S. enteritidis derivative EL23 produced heat-labile enterotoxin subunit B that was indistinguishable from heat-labile enterotoxin subunit B produced by strains of E. coli or Salmonella typhi harboring the same plasmid. Mice immunized orally with strain EL23 developed progressively increasing mucosal and serum antibody responses to both heat-labile enterotoxin subunit B and to the lipopolysaccharide of the vaccine strain. The mucosal antibody response was shown to be immunoglobulin A specific and to be capable of neutralizing the biological activities of both E. coli heat-labile enterotoxin and cholera enterotoxin in vitro.
D000284:Administration, Oral; D000818:Animals; D000992:Antitoxins / Q000032:analysis; D001427:Bacterial Toxins / Q000235:genetics* / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D002772:Cholera Toxin / Q000235:genetics; D004274:DNA, Recombinant; D004768:Enterotoxins / Q000235:genetics* / Q000276:immunology; D029968:Escherichia coli Proteins; D005260:Female; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D012477:Salmonella enteritidis / Q000235:genetics / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology
D000992:Antitoxins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004274:DNA, Recombinant; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D014613:Vaccines, Attenuated; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli