title: Separation of human lymphoid cells by affinity chromatography and cell surface labelling by hydroxyethyl methacrylate particles using monoclonal antibodies.
abstract: The hydroxyalkyl methacrylate gel Separon, Sepharose 6MB and polystyrene dishes with bound antibodies were used for affinity chromatography of cells isolated from human tonsils and peripheral blood. Enrichment of T cells by filtration through anti-human immunoglobulin Separon or Sepharose 6MB columns was comparable to that achieved by "panning" on dishes or by Nylon wool column fractionation. High enrichment of B cells was obtained when cells pretreated with monoclonal anti-T cell antibodies were fractionated on columns or dishes where anti-mouse antibody was bound. Hydroxyethyl methacrylate copolymer microspheres were conjugated with monoclonal antibodies directed against surface markers of human lymphocytes or with anti-mouse immunoglobulin antibodies and used for surface labelling of cells. The results obtained by immunofluorescence were in good correlation with labelling by hydroxyethyl methacrylate immunobeads.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002469:Cell Separation / Q000379:methods; D002846:Chromatography, Affinity; D006801:Humans; D007124:Immunoenzyme Techniques; D007164:Immunosorbents; D008214:Lymphocytes / Q000166:cytology*; D008689:Methacrylates; D014066:Palatine Tonsil / Q000166:cytology; D013499:Surface Properties; D013552:Swine; D013936:Thymidine / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D007164:Immunosorbents; D008689:Methacrylates; C005044:hydroxyethyl methacrylate; D013936:Thymidine