title: [Increase in the coefficient of component utilization by balancing the composition of the nutrient medium for growing pneumococci].
abstract: The possibility of using the experimental-analytical balance method (EABM) for development of balanced media, optimal and economic by their composition is shown. The method is based on the specific growth activity of the medium components and mathematical calculation of their concentrations. A balanced medium containing human placenta hydrolysate was developed. The medium has a high component consumption coefficient and provides maintenance of high levels of the pneumococcal culture biological properties such as growth, capsule formation, virulence and agglutinability. The quantities of nutrient yeast extract, cystine, glucose and sodium chloride in the balanced medium are 2, 18-20, 4-4.7 and 32-33 times lower respectively than those in the initial medium. The use of the balanced medium instead of the initial unbalanced one or instead of the meat medium based on the Hottinger broth will provide the total economy of 1010 or 2360-2580 roubles respectively per. 1000 liters of the medium. It is concluded that a wider use of this method in optimization of microbiological nutrient media in production of medical and biological preparations is promising.
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D010920:Placenta; D011247:Pregnancy; D011492:Protein Hydrolysates / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000254:growth & development* / Q000472:pathogenicity
chemical_list: D003470:Culture Media; D011492:Protein Hydrolysates
vernacular_title: Povyshenie koéffitsienta ispol'zovaniia komponentov pri balansirovke sostava pitatel'noĭ sredy dlia vyrashchivaniia pnevmokokkov.