Demonstration of clonable alloreactive host T cells in a primate model for bone marrow transplantation.
The phenomenon of marrow rejection following supralethal radiochemotherapy was explained in the past mainly by non-T-cell mechanisms known to be resistant to high-dose irradiation. In the present study a low but significant number of radiochemoresistant-clonable T cells was found in the peripheral blood and spleen of Rhesus monkeys following the cytoreductive protocol used for treatment of leukemia patients prior to bone marrow transplantation. More than 95% of the clonable cells are concentrated in the spleen 5 days after transplant. The cells possess immune memory as demonstrated by the generation of alloreactive-specific cytotoxicity. The present findings suggest that host-versus-graft activity may be mediated by alloreactive T cells. It is hoped that elimination of such cells prior to bone marrow transplantation will increase the engraftment rate of HLA-nonidentical marrow in leukemia patients.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002453:Cell Cycle; D002999:Clone Cells; D003520:Cyclophosphamide / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D007519:Isoantigens / Q000276:immunology; D008198:Lymph Nodes / Q000528:radiation effects; D008253:Macaca mulatta; D008297:Male; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000528:radiation effects; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology / Q000528:radiation effects; D014916:Whole-Body Irradiation
D007519:Isoantigens; D003520:Cyclophosphamide