[Bacteriosorption reaction on a smooth surface for detecting antibodies and antigens].
The solid-phase technique for the detection of antibodies and antigens has been developed and named the bacteriosorption test. The test is based on binding staphylococci containing protein A with the Fc-regions of IgG-antibodies attached to antigens immobilized on polystyrene. The possibilities of this technique have been analyzed with the use of diphtheria toxoid, house-dust allergen and homologous rabbit antisera. In the detection of antibodies the proposed test is not inferior to the passive hemagglutination test, and its sensitivity in the detection of antigens by the sandwich technique reaches 0.05-0.1 micrograms/ml. The specificity of the technique has been experimentally confirmed by the inhibition of the reaction with soluble antigen and staphylococcal protein A. The variability factor of the technique does not exceed 10%.
D000327:Adsorption; D000485:Allergens / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000032:analysis*; D004168:Diphtheria Toxoid / Q000276:immunology; D004391:Dust; D006386:Hemagglutination Tests; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D007117:Immunization, Secondary; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D007158:Immunologic Techniques / Q000295:instrumentation; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000276:immunology; D013210:Staphylococcus / Q000276:immunology*; D013997:Time Factors
D000485:Allergens; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004168:Diphtheria Toxoid; D004391:Dust; D007106:Immune Sera; D007141:Immunoglobulin Fc Fragments; D013205:Staphylococcal Protein A
Reaktsiia bakteriosorbtsii na gladkoĭ poverkhnosti dlia vyiavleniia antitel i antigenov.