A chromium release assay for the assessment of spontaneous and immune hemolysis of heterologous erythrocytes by sera of rainbow trout.
This report describes the development of a microtiter system for measuring spontaneous hemolytic activity and immune hemolysins in rainbow trout serum. The chromium release assay, utilizing complement-mediated lysis of heterologous erythrocytes allows rapid, efficient quantitation of hemolytic activity in small serum samples from numerous fish.
D000818:Animals; D002645:Chickens; D002860:Chromium Radioisotopes; D004912:Erythrocytes / Q000276:immunology / Q000502:physiology*; D006386:Hemagglutination Tests; D006461:Hemolysis; D006462:Hemolytic Plaque Technique; D006801:Humans; D012487:Salmonidae / Q000097:blood*; D012756:Sheep; D014337:Trout / Q000097:blood*
D002860:Chromium Radioisotopes