A plaque assay for cells making antibody against HLA antigens.
A plaque assay has been devised, for detecting cells making antibodies to HLA antigens, using intact nucleated cells as targets. The principle is to use a green fluorescent dye to stain living cells and a red fluorescent dye to stain the cells in a plaque that have been lysed by antibody and complement. Plaques are counted under a fluorescence microscope and the preparations can be made permanent and virtually non-quenchable by drying.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology*; D002460:Cell Line; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007158:Immunologic Techniques; D007518:Isoantibodies / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D007518:Isoantibodies