title: A monoclonal antibody labels type II neurons of the spiral ganglion.
abstract: Tissue from mature rat and cat cochleas was stained with basic dyes in order to distinguish between the cell bodies of type I and II spiral ganglion neurons. This same tissue was then stained with protargol, or processed immunocytochemically using a monoclonal neurofilament antibody, RT-97. Type II neurons were intensely stained, whereas type I neurons were not. We conclude that type II neurons can be clearly distinguished by their abundant neurofilament content.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003051:Cochlea / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D012836:Silver Proteins; D013136:Spiral Ganglion / Q000166:cytology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D012836:Silver Proteins; C003232:strong silver protein