Purification of human plasma inactive renin by immunoaffinity chromatography on profragment-specific IgG.
Inactive renin was purified to apparent homogeneity from human plasma by ion exchange, gel filtration, Affi-Gel blue, immunoaffinity chromatography on profragment-specific IgG coupled to Sepharose, and preparative HPLC. By this method, a 460000-fold purification was obtained. The purified renin was totally inactive and was activated by trypsin.
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D012083:Renin / Q000276:immunology / Q000302:isolation & purification*
D007074:Immunoglobulin G; D012083:Renin