[Gluconeogenesis, ketogenesis, and ureogenesis in isolated hepatocytes from diabetic rats: effects of insulin].
The intermediary metabolism in hepatocytes isolated from diabetic rats has been studied. The incubation medium is a Krebs-Henseleit buffer containing albumin-bound oleate (1 mmol/l). The high rates of gluconeogenesis, ureogenesis and ketogenesis were consistent with the diabetic state of the rats. Insulin (8 X 10(-7) mol/l) decreased glucose and urea productions from alanine (10 mmol/l) by 30%; beta-hydroxybutyrate production, oleate utilization and malate efflux (calculated) from mitochondria were also decreased. No effect of insulin was found with lactate (10 mmol/l) as gluconeogenic substrate. The glycogen content of cells was constant during the time of incubation (4h). These data suggest that the oxidation-reduction state of mitochondria and the cytoplasmic oxaloacetate concentration could be important factors in the action of insulin.
D000409:Alanine / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007657:Ketone Bodies / Q000096:biosynthesis*; D007773:Lactates / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D051381:Rats; D014508:Urea / Q000096:biosynthesis*
D007328:Insulin; D007657:Ketone Bodies; D007773:Lactates; D009829:Oleic Acids; D019301:Oleic Acid; D014508:Urea; D005947:Glucose; D000409:Alanine
Gluconéogénèse, cétogénèse et uréogénèse dans les hépatocytes isolés de rats diabétiques: effets de l'insuline.