Fed-batch cultivation of Saccharomyces cerevisiae with increased content of delta 5,7-sterols.
Cultivation of Saccharomyces cerevisiae with a linear nutrient feed was used to test the validity of a mathematical model describing changes in the physiological state of the culture. Markers of these changes were the concentrations of proteins and delta 5,7-sterols in yeast dry mass. The model was used to optimize the production of these sterols with regard to the magnitude and composition of nutrient feed.
D003201:Computers; D003470:Culture Media; D000431:Ethanol / Q000032:analysis; D005656:Fungal Proteins / Q000032:analysis; D008954:Models, Biological; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism; D013261:Sterols / Q000032:analysis / Q000096:biosynthesis*
D003470:Culture Media; D005656:Fungal Proteins; D013261:Sterols; D010758:Phosphorus; D000431:Ethanol; D009584:Nitrogen