[Activity of cathepsin B and serine trypsin-like peptide hydrolases in human kidney extracts].
A potentiality to use the available substrates BAPNA and BAME was studied in estimation of activities of cathepsin B and other trypsin-like hydrolases in extracts of human kidney cortex. In these extracts the BAME-hydrolyzing activity of cathepsin B was difficult if impossible to detect due to high level of attendant non-thiol esterases. At the same time, BAPNA might be used for this purpose as a substrate in estimation of cathepsin B- and trypsin-like peptide hydrolase activities in biopsies of human kidney.
D001587:Benzoylarginine-2-Naphthylamide / Q000378:metabolism; D002401:Cathepsin B; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; D013379:Substrate Specificity; D014020:Tissue Extracts / Q000032:analysis; D014357:Trypsin / Q000378:metabolism
D011480:Protease Inhibitors; D014020:Tissue Extracts; D001587:Benzoylarginine-2-Naphthylamide; D002403:Cathepsins; D010450:Endopeptidases; D012697:Serine Endopeptidases; D014357:Trypsin; D002401:Cathepsin B
Aktivnost' katepsina B i serinovykh tripsinpodobnykh peptidgidrolaz v ékstraktakh pochki cheloveka.