title: Rearrangement of tubulin, actin, and myosin in cultured ventricular cardiomyocytes of the adult rat.
abstract: Antitubulin, phalloidin, and antimyosin were used to study the distribution of microtubules, microfilaments, and myofibrils in cultured adult cardiomyocytes. These cells undergo a stereotypic sequence of morphological change in which myotypic features are lost and then reconstructed during a period of polymorphic growth. Microtubules, though rearranged during these events in culture, are always present in an organized network. Myosin and actin structures, on the other hand, initially degenerate. This initial degeneration is reversed when a cell attaches to the culture substratum. Upon attachment, new microtubules are laid down as a cortical network adjacent to the sarcolemma and, subsequently, as a network in the basal part of the cell. Actin and then myosin filament bundles appear next, in a pattern corresponding to the pattern of the microtubules. Finally, striated myofibrils are formed, first in the central part of the cell, and subsequently in the outgrowing processes of the cell. A mechanism is suggested by which the eventual polymorphic shape of a cell is related to the shape of its initial area of contact with the culture substratum. Finally, a model of myofibrillogenesis is proposed in which microtubules participate in the insertion of myosin among previously formed actin filament bundles to produce myofibrils.
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006352:Heart Ventricles / Q000166:cytology; D007106:Immune Sera; D008297:Male; D009206:Myocardium / Q000166:cytology*; D009218:Myosins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014404:Tubulin / Q000032:analysis*
chemical_list: D000199:Actins; D007106:Immune Sera; D014404:Tubulin; D009218:Myosins