title: Differential recognition patterns of human immunoglobulin classes to antigens of Onchocerca gibsoni.
abstract: Adult Onchocerca gibsoni worms were fractionated into a surface-enriched fraction, a saline extract, a saline insoluble-detergent soluble fraction and a total glycoproteins extract. The antigens in each fractions were separated by electrophoresis in polyacrylamide gels and examined with an immuno-blot technique for reactive antibodies in sera from individuals infected with a variety of filarial and non-filarial nematode worms. Radiolabelled monoclonal antibodies were used to determine the Ig heavy chain isotypes. A number of antigens were demonstrated in all of the extracts, with many antigens of each extract being unique. Although some Onchocerca antigens stimulated antibodies of all human immunoglobulin classes, the panel of antigens recognized by each Ig isotype was different. The IgE response was restricted and directed at antigens not recognized by antibodies to other nematode parasites. IgM and IgA responses tended to recognize many antigens, whilst IgG responses were directed at intermediate numbers of antigens. The control of isotype balance to individual parasite antigens is thus independently regulated. This survey provides a rational basis for the exploration of Onchocerca antigen-human antibody class systems with relevance for diagnosis, protection and pathology.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology*; D007158:Immunologic Techniques; D009854:Onchocerca / Q000032:analysis / Q000276:immunology*; D009855:Onchocerciasis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007073:Immunoglobulin E