title: The molecular basis of the origin of complete and mosaic mutants.
abstract: To study the molecular basis of the origin of complete and mosaic mutants, pBR322 plasmids with damage to one or both DNA strands were constructed by limited chemical modification of plasmid DNA. Damage to one strand of DNA resulted in the induction of predominantly mosaic mutants. Data were obtained indicating that complete mutations arise as a result of damage to both strands in the region of the mutated gene.
mesh_terms: D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D009030:Mosaicism; D009154:Mutation; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial