title: The complete amino acid sequence of vitelline coat lysin.
abstract: The vitelline coat lysin of a top shell, Tegula pfeifferi, is a single polypeptide consisting of 118 amino acid residues and having a relative molecular mass of 13800. The complete amino acid sequence of the vitelline coat lysin was determined by the analyses of five peptides obtained by cyanogen bromide degradation and three fragments obtained by Staphylococcus aureus protease digestion of the protein. The sequence showed the presence of microheterogeneities in the vicinity of the C-terminal half of the molecule and the existence of two homologous domain structures.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide; D010450:Endopeptidases / Q000378:metabolism; D008297:Male; D008974:Mollusca / Q000201:enzymology*; D009088:Mucoproteins; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D012697:Serine Endopeptidases; D013094:Spermatozoa / Q000201:enzymology*; D014817:Vitelline Membrane / Q000378:metabolism
chemical_list: D009088:Mucoproteins; D010446:Peptide Fragments; C010393:lysin, gastropoda; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D003488:Cyanogen Bromide