In vivo time course of synthesis and processing of major schizont membrane polypeptides in Plasmodium falciparum.
A rapid method of separating membrane co-sedimentable and soluble components of Plasmodium infected erythrocytes is presented. We propose a nomenclature for major P. falciparum polypeptides, applicable to different isolates and based on their cellular location and stage specificity. For four of these polypeptides (185 kDa = Mp1; 120 kDa = Mp3; 76 kDa = Mp5; 90 kDa= Sp2) supposed to play a role in protective immunity, monospecific antibodies were available. We have studied their fate at the time of merozoïte release and reinvasion, and the possible correlations between these polypeptides, by pulse-chase experiments.
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000378:metabolism*; D000954:Antigens, Surface / Q000096:biosynthesis / Q000378:metabolism*; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis / Q000469:parasitology; D006801:Humans; D008854:Microscopy, Electron; D010452:Peptide Biosynthesis; D010455:Peptides / Q000276:immunology / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000276:immunology*; D011499:Protein Processing, Post-Translational
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D010455:Peptides