Triton X-114 phase separation of platelet membrane glycoproteins from normal subjects and a patient with type I thrombasthenia.
Surface-labelled normal and thrombasthenic platelets have been subjected to phase separation in Triton X-114. Triton-rich and Triton-poor fractions have been analysed by SDS-PAGE and IEF-SDS-PAGE. Partitioning characteristics of the major glycoproteins have been defined. The Triton-rich fraction contained GPIIb, III, IV, VI, VII, VIII, GP38 and the IIb beta subunit. In contrast, the Triton-poor fraction contained the HMWGP, GPIa, Ib, IIb, III, V and GPIX. Analysis of the platelet membrane glycoproteins of a patient with Type 1 thrombasthenia has been carried out using Triton X-114. The value of the method in diagnosis of this condition and differences between our findings and those published previously are discussed.
D001791:Blood Platelet Disorders / Q000097:blood*; D001792:Blood Platelets / Q000032:analysis*; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000097:blood*; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D012967:Sodium Dodecyl Sulfate; D013915:Thrombasthenia / Q000097:blood*
D006023:Glycoproteins; D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40