SOS induction of the gene sulA is partly inhibited in Escherichia coli K12 cells overproducing the RecA protein.
A study was made of the SOS induction of the gene sulA of Escherichia coli K12 in relation to the gene dosage of the gene recA. In experiments the sulA::lacZ fusion strain PQ37 and derivatives of PQ37 with the multi-copy plasmids pDR1453 or pBR322 were used. The SOS response was induced with nitrofurantoin, SOS induction of the gene sulA was determined on the basis of the amount of beta-galactosidase synthesized, i.e. by the SOS chromotest (Quillardet et al., 1982a). It was found in this work that cells with the plasmid pDR1453, which contain the gene recA of E. coli K12 (Sancar and Rupp, 1979), have a decreased SOS induction of the gene sulA. Cells with the plasmid pBR322 do not exhibit this decrease. Inactivation of the gene recA in the plasmid pDR1453 with preservation of the functional gene recA in the chromosome leads to a restoration of 'standard' SOS induction of the gene sulA. The results show that the amount of the gene product of the gene recA affects the SOS induction of the gene sulA.
D004260:DNA Repair / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009582:Nitrofurantoin / Q000494:pharmacology; D010957:Plasmids; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000235:genetics*
D009582:Nitrofurantoin; D011938:Rec A Recombinases