Identification of c-myb (chicken), c-myb (mouse) and v-myb (AMV) protein products by immunoprecipitation with antibodies directed against a synthetic peptide.
A synthetic nonadecapeptide (IL 19) derived from a sequence of v-myb was covalently bound to haemocyanin and used for immunization. Anti-IL 19 serum immunoprecipitated a 75 kDa protein in the lysate of metabolically labelled chicken and murine thymus cells. Presaturation of the serum with IL 19 abolished this immunoprecipitation, thus indicating that the product of c-myb in both chicken and murine thymuses is the 75 kDa protein (p75c-myb). Anti IL 19 serum also precipitated p48v-myb in the lysate of nonproducer myeloblasts.
D000818:Animals; D009189:Avian Myeloblastosis Virus; D002472:Cell Transformation, Viral; D011232:Chemical Precipitation; D002645:Chickens; D006241:Haptens / Q000276:immunology; D007120:Immunochemistry; D051379:Mice; D020626:Oncogene Proteins v-myb; D010779:Photofluorography; D011518:Proto-Oncogene Proteins / Q000276:immunology / Q000302:isolation & purification*; D020598:Proto-Oncogene Proteins c-myb; D012191:Retroviridae Proteins / Q000276:immunology / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis
D006241:Haptens; D020626:Oncogene Proteins v-myb; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012191:Retroviridae Proteins