Detection of double-stranded RNA by immunoblot electrophoresis.
A procedure is described which permits the serological detection of double-stranded RNA (dsRNA) which has been electrophoretically transferred from agarose gels to Zeta-Probe blotting membranes. Cucumber mosaic virus replicative forms of RNAs 1-3 and cassava (Manihot esculenta) clone 'Secundina' dsRNAs can be routinely detected from 1 g of leaf tissue. This procedure allows for the detection and size estimation of dsRNAs in crude nucleic acid preparations.
D004587:Electrophoresis, Agar Gel; D007158:Immunologic Techniques; D009029:Mosaic Viruses / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*
D012330:RNA, Double-Stranded; D012367:RNA, Viral