Stage-specific insulin binding in mouse preimplantation embryos.
Stage-specific insulin binding in the developing mouse embryo was demonstrated by an indirect immunofluorescence technique using an antibody against insulin. Concentration-dependent fluorescence labeling was observed in the morula and blastocyst stages of development, whereas no reactivity was seen in unfertilized oocytes or in 2-, 4-, and 8-cell embryos. The possible significance of these observations is discussed. This represents the first report of stage-specific insulin binding during mammalian preimplantation development.
D000818:Animals; D047108:Embryonic Development; D005260:Female; D005455:Fluorescent Antibody Technique; D007328:Insulin / Q000378:metabolism*; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D011247:Pregnancy; D015053:Zygote / Q000378:metabolism*
D007328:Insulin