Characterization of a monoclonal antibody recognizing a monomorphic determinant of the alpha chain of class II DQ antigens.
We have characterized a monoclonal antibody (L2) specific for a determinant of Human DQ class II antigens. After immunoprecipitation and immunofluorescence analysis performed on a panel of DR homozygous cell lines and HLA deleted mutants, it behaves like a monomorphic anti-DQ reagent and does not react with the products of the various DR alleles. On immunoblotting, it reacts with an epitope of the DQ alpha chain.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D011232:Chemical Precipitation; D005455:Fluorescent Antibody Technique; D006683:HLA-DQ Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006683:HLA-DQ Antigens; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances