New method of hepatocyte transplantation and extracorporeal liver support.
A technique has been developed by the authors that allows hepatocyte attachment on collagen-coated microcarriers resulting in prolonged hepatocyte viability and function both in vivo and in vitro. Rat hepatocytes were obtained by portal vein collagenase perfusion. Intraperitoneally transplanted microcarrier-attached normal hepatocytes into congeneic Gunn rats were functioning 3-4 weeks later, as shown by the presence and persistence of conjugated bilirubin in recipient bile, sustained decrease in serum bilirubin, uptake of Tc99m-DESIDA, and morphologic criteria. Intraperitoneal transplantation of normal microcarrier-attached hepatocytes into genetically albumin deficient rats (NAR) resulted in marked increase in plasma albumin levels (6 days without and 21 days with Cyclosporin A immunosuppression). Microcarrier-attached hepatocytes transplanted after 2 weeks of storage at -80 C into congeneic Gunn rats were viable and functional as assessed by criteria outlined above. An extracorporeal liver perfusion system was developed using the microcarrier-attached hepatocytes that was capable of synthesizing and conjugating bilirubin and synthesizing liver-specific proteins.
D000818:Animals; D001646:Bile / Q000378:metabolism; D001663:Bilirubin / Q000032:analysis / Q000378:metabolism; D002448:Cell Adhesion; D002855:Chromatography, Thin Layer; D006085:Graft Survival; D008099:Liver / Q000166:cytology; D016031:Liver Transplantation; D008297:Male; D008722:Methods; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011758:Pyrroles / Q000378:metabolism; D051381:Rats; D011911:Rats, Gunn; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism; D014021:Tissue Preservation / Q000379:methods
D011758:Pyrroles; D012709:Serum Albumin; D001663:Bilirubin