Prostacyclin production by the bovine aortic smooth muscle.
It is well known that cultured aortic smooth muscle cells, the phenotype of which has modulated from contractile to synthetic, are able to release prostacyclin (PGI2). We have studied the release of PGI2 from cultured explants of bovine aortic media, which represent an homogeneous population of smooth muscle cells with a contractile phenotype. These explants released spontaneously huge amounts of PGI2, which was the major eicosanoid produced. PGI2 release was stimulated by serum and by serotonin. This experimental model seems useful to evaluate the contribution of smooth muscle to the biosynthesis of PGI2 by the arterial wall.
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D011464:Epoprostenol / Q000096:biosynthesis*; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D009924:Organ Culture Techniques; D012701:Serotonin / Q000494:pharmacology
D012701:Serotonin; D011464:Epoprostenol