title: Hybrid formation within the genus Leishmania?
abstract: Leishmanial organisms isolated from a desert rodent (Psammomys obesus) and a feral dog (Canis familiaris) in the Eastern Province of Saudi Arabia were isoenzymically distinct from Leishmania major and L. arabica, organisms usually associated with human and wild animal cutaneous leishmaniasis in this area. Further examination of isoenzyme banding patterns of cloned populations of these organisms, together with karyotyping using orthogonal field alternation gel electrophoresis and the use of highly specific kinetoplast DNA probes, has produced evidence suggesting that these organisms isolated from the dog and the rodent are hybrids of L. major and L. arabica.
mesh_terms: D000818:Animals; D003411:Arvicolinae; D015342:DNA Probes; D004285:Dogs; D004586:Electrophoresis; D006824:Hybridization, Genetic; D007527:Isoenzymes / Q000032:analysis*; D007891:Leishmania / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D015342:DNA Probes; D007527:Isoenzymes