Screening pesticides for their ability to damage bacterial DNA.
Twenty-six pesticides and pesticide degradation products were screened (125 micrograms - 2000 micrograms) for their ability to induce unrepairable damage to bacterial DNA. Three repair test systems were utilized in this study, the Salmonella typhimurium (TA1538/TA1978), the E. coli K-12 (Pol A1+/Pol1-) and the E. coli WP2 (WP2, WP2uvrA, WP67, CM611 and CM571). Aldicarb (1000 micrograms), benomyl (250 micrograms), 2-aminobenzimidazole (2000 micrograms), captan (125 micrograms), fenazalor (500 micrograms), 5,6-dichloro-2-trifluoromethylbenzimidazole (NC-2983) (250 micrograms), isothymol (250 micrograms), maleic hydrazide (1000 micrograms), pentachloronitrobenzene (1000 micrograms) were DNA-damaging to one or more bacterial test systems. Isothymol and NC-2983 affected all three test systems. Chlorinated hydrocarbon insecticides, some being recognized as carcinogens, did not produce a zone of inhibition in any of the tester strains possibly due to their poor solubility and diffusion in the agar overlay. It was concluded that these tests can be performed along with bacterial reversion tests to complement each other as short-term screening tests for potential carcinogens and mutagens.
D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D010575:Pesticides / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
D004269:DNA, Bacterial; D010575:Pesticides