Comparison of four test procedures to identify Staphylococcus aureus isolated from bovine intramammary infections.
A comparison was made between conventional tube coagulase, macrocupsular coagulase, latex agglutination, and miniaturized biochemical test systems for identification of Staphylococcus aureus of bovine origin. A total of 303 gram-positive, catalase-positive cocci of bovine origin were tested. Agreement between each pair of 4-hour tube coagulase, macrocupsular coagulase, latex agglutination, and miniaturized biochemical test results within isolates was greater than 95.0. Seventeen (5.6%) isolates were test negative for 4-hour tube coagulase, but test positive for 24-hour tube coagulase. Thirteen (76.5%) of these isolates were identified as S hyicus, 3 were S aureus, and 1 was not identified.
D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary*; D002417:Cattle; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000302:isolation & purification*