[Aspartase activity of Escherichia coli, strain 85, cells grown on glucose-mineral medium with yeast extract].
The conditions for cultivation of E. coli 85 on a glucose-mineral medium with a yeast extract were optimized. The cells obtained had a high aspartate ammonia-lyase activity. The aspartase activity was determined kinetically both by consumption of the substrate, ammonium fumarate, and by accumulation of the product, aspartic acid.
D000642:Ammonia-Lyases / Q000378:metabolism*; D001220:Aspartate Ammonia-Lyase / Q000378:metabolism*; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005947:Glucose; D008903:Minerals; D015003:Yeasts
D003470:Culture Media; D008903:Minerals; D000642:Ammonia-Lyases; D001220:Aspartate Ammonia-Lyase; D005947:Glucose
Aspartaznaia aktivnost' kletok Escherichia coli, shtamm 85, vyrashchennykh na gliukozomineral'noĭ srede s drozhzhevym ékstraktom