Na+-dependent p-aminohippurate transport at the basolateral side of the isolated perfused rat kidney.
The uptake of p-amino[3H]hippurate by isolated perfused rat kidney was studied to characterize the mechanism which was responsible for organic anion transport process. A rapid injection multiple indicator dilution technique and the distributed two-compartment model of Sawada et al. (Computer Methods Programs Biomed., 20 (1985) 51) were employed. Some characteristics of a carrier-mediated transport from the antiluminal space to the intracellular space for p-aminohippurate at the basolateral side were demonstrated: the uptake was stimulated by the countertransport effect and showed Na+ dependency. These findings are consistent with p-amino[3H]hippurate's being taken up into the isolated rat basolateral membrane vesicle by Na+-dependent carrier-mediated transport (J. Pharmacol. Exp. Ther. 227 (1983) 122). It is suggested that the multiple indicator dilution technique is a sensitive new method to study the mechanisms of renal tubular transport in the living kidney as an organ.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D007201:Indicator Dilution Techniques; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology*; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D012964:Sodium; D010130:p-Aminohippuric Acid