Reactivation of nerve agent inhibited human acetylcholinesterases by HI-6 and obidoxime.
Acetylcholinesterase was purified from human caudate nucleus and skeletal muscle. The enzyme preparations were used to study aging and reactivation by HI-6 and obidoxime after inhibition by soman and its isomers. HI-6 was found to be the most potent reactivator. For both enzyme preparations a higher reactivatability and a higher rate of aging were observed after inhibition by C+-soman than after inhibition by C(-)-soman. Aging was retarded by propidium diiodide. Reactivation by the two oximes was also studied after inhibition by tabun, sarin and VX. Tissue homogenates were used for this part of the work. Our conclusion is that HI-6 is superior to obidoxime for human acetylcholinesterases inhibited by soman and sarin, while obidoxime is better towards tabun-inhibited enzyme.
D000110:Acetylcholinesterase / Q000032:analysis / Q000302:isolation & purification; D002421:Caudate Nucleus / Q000201:enzymology; D002801:Cholinesterase Reactivators / Q000494:pharmacology*; D004491:Edrophonium / Q000494:pharmacology; D006801:Humans; D009132:Muscles / Q000201:enzymology; D009768:Obidoxime Chloride / Q000494:pharmacology*; D010755:Organophosphates / Q000633:toxicity; D009946:Organothiophosphorus Compounds / Q000633:toxicity; D010091:Oximes / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D012524:Sarin / Q000633:toxicity; D012999:Soman / Q000633:toxicity; D013237:Stereoisomerism
D000110:Acetylcholinesterase; D002801:Cholinesterase Reactivators; D004491:Edrophonium; D009768:Obidoxime Chloride; D010755:Organophosphates; D009946:Organothiophosphorus Compounds; D010091:Oximes; D011726:Pyridinium Compounds; D012524:Sarin; D012999:Soman; C009680:VX-agent; C022870:asoxime chloride; C009374:tabun