An in situ immunoperoxidase staining procedure for human cell colonies grown in semi-solid agar culture.
An adaptation of the immunoperoxidase staining procedure is described which enables the detection of antigens expressed by human hemopoietic cells grown in semi-solid agar culture. Positive staining was achieved using several different monoclonal antibodies recognizing an array of cell surface antigens on developing myeloid cells.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002999:Clone Cells / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface