title: Luminal uptake and intracellular transport of insulin in renal proximal tubules.
abstract: It is generally accepted that proteins taken up from the renal tubular fluid are transported into lysosomes in proximal tubule cells. Recently, however, it has been postulated that insulin in isolated perfused rat kidneys did not accumulate in lysosomes but to a certain degree in the Golgi region. The present study was undertaken to investigate the intracellular handling of biologically unaltered insulin in rat renal proximal tubule cells. Rats were prepared for in vivo micropuncture and either a colloidal gold insulin complex or insulin monoiodinated in the A-14 position (125I-insulin) was microinfused into proximal tubules. After 5, 10, 25 or 60 min the tubules were fixed by microinfusion of glutaraldehyde and processed for electron microscopy or electron microscope autoradiography. A qualitative analysis of tubules infused with colloidal gold insulin or 125I-insulin showed that insulin was taken up by endocytosis and transported to lysosomes, and a quantitative autoradiographic analysis of the 125I-insulin microinfused tubules showed that the grain density after five min was significantly increased for endocytic vacuoles and for lysosomes. After 60 min the grain density was still significant over lysosomes. The accumulation of grains was non-significant over all other areas analyzed at any time. This study shows that insulin is taken up from the luminal side of the proximal tubule by endocytosis and transported to the lysosomes. There was no significant transport to the Golgi region.
mesh_terms: D000818:Animals; D001345:Autoradiography; D001692:Biological Transport; D003102:Colloids; D004705:Endocytosis; D006046:Gold; D007328:Insulin / Q000031:analogs & derivatives / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D014617:Vacuoles / Q000378:metabolism
chemical_list: D003102:Colloids; D007328:Insulin; D007457:Iodine Radioisotopes; C008147:insulin, iodo-; D006046:Gold