title: Electron microscopic localization of glutamate dehydrogenase in rat liver mitochondria by an immunogold procedure and monoclonal and polyclonal antibodies.
abstract: Glutamate dehydrogenase (GDH) was localized in rat liver by indirect electron microscopic immunogold, using different sizes of gold particles and monoclonal and polyclonal antibodies. Using the protein A-gold technique in double immunocytochemical experiments, both antibodies, at their optimal dilutions, gave similar results. A novel assessment of the distribution of GDH was made by measurements of the number of gold particles per square micrometer of cross-sectional images of individual mitochondria. The data indicate intracellular homogeneity among mitochondria in individual parenchymal cells. The enzyme is almost absent in non-parenchymal cells. Finally, GDH was found mainly in association with the mitochondrial inner membrane.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002451:Cell Compartmentation; D005969:Glutamate Dehydrogenase / Q000032:analysis* / Q000276:immunology; D006046:Gold; D006651:Histocytochemistry; D007158:Immunologic Techniques; D007425:Intracellular Membranes / Q000276:immunology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013205:Staphylococcal Protein A
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D013205:Staphylococcal Protein A; D006046:Gold; D005969:Glutamate Dehydrogenase