title: Cytogenetic markers in allogeneic bone marrow transplantation.
abstract: Engraftment after bone marrow transplantation (BMT) can be monitored with genetic markers. Cytogenetic markers are the ones that can be studied earliest. Of 27 patients studied, 16 had a sex-mismatched donor, and in 8 cases, there were discriminative markers identified with regular Giemsa and Q-banding. In one case, there was a rather slight polymorphism of the C-bands of chromosomes #9. Thus, donors' cells were identifiable in 25 of 27 cases (93%). The markers studied easiest are the sex chromosomes #3 and characteristic satellites of acrocentric chromosomes are rather frequent and also well suited to discriminate donors' from patients' cells. Nonprominent variabilities (fluorescence of the centromeric regions of chromosomes #4 and #6 after Q-banding), rare polymorphisms (pericentric inversions of chromosomes #1 and #9), and difficult to evaluate polymorphisms (heterochromatic regions of chromosomes #1, #9, and #16) are not very practical for the routine monitoring of engraftment after BMT.
mesh_terms: D016026:Bone Marrow Transplantation; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D012730:Sex Chromosomes