Basement membrane components produced by a mouse ascites teratocarcinoma TB24. Analysis with monoclonal and polyclonal antibodies.
Monoclonal antibodies were produced against two basement membrane glycoproteins, laminin and entactin. The specificity of the antibodies and the absence of cross-reactivity were established by radio-immunoprecipitation of mouse ascites teratocarcinoma cell lysates. This teratocarcinoma, TB24, formed large embryoid bodies filled with extracellular matrix. The major component of the matrix was laminin. Abundant entactin, substantial amounts of fibronectin and rather little collagen type IV were also found in the matrix by biochemical and immunofluorescence analyses. TB24 teratocarcinoma appears to be a good model to test anti-basement membrane antibodies and to isolate certain extracellular matrix components in preparative quantities.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001485:Basement Membrane / Q000276:immunology*; D002460:Cell Line; D004727:Endothelium / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005109:Extracellular Matrix / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D007797:Laminin / Q000276:immunology* / Q000302:isolation & purification; D008562:Membrane Glycoproteins; D051379:Mice; D008854:Microscopy, Electron; D013724:Teratoma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D007797:Laminin; D008562:Membrane Glycoproteins; C029967:nidogen