title: The complete amino acid sequence of human brain-derived acidic fibroblast growth factor.
abstract: Acidic fibroblast growth factor is a potent mitogen for a variety of cells in culture, including vascular endothelial cells, and is angiogenic in vivo. The complete amino acid sequence of human brain-derived acidic fibroblast growth factor has been determined from amino terminal sequence analysis and carboxypeptidase A digestion of the whole protein and sequence analyses of peptides generated by tryptic, Staphylococcus aureus V8 protease and cyanogen bromide cleavages. A potential Asn-Gly-Ser glycosylation sequence is present in the human protein. The complete amino acid sequence is compared to that of the equivalent protein purified from bovine brain.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D010450:Endopeptidases; D005346:Fibroblast Growth Factors / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010446:Peptide Fragments / Q000032:analysis; D012697:Serine Endopeptidases; D013045:Species Specificity; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D005346:Fibroblast Growth Factors; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin