Oligonucleotide directed mutagenesis of cauliflower mosaic virus DNA using a repair-resistant nucleoside analogue: identification of an agnogene initiation codon.
Mutation of the initiation codon of the dispensible open reading frame, ORF VII, of cauliflower mosaic virus (CaMV) delayed the appearance of disease symptoms, but the mutants reverted with high frequency. This suggests a role of this start codon in viral expression. Oligonucleotide-directed mutagenesis, utilizing a novel, repair-resistant deoxyguanosine analogue, 2'-deoxy-7-deazainosine (dDI), highly improved the yield of mutants.
D001483:Base Sequence; D001937:Brassica; D003062:Codon; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D009029:Mosaic Viruses / Q000187:drug effects / Q000235:genetics*; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D010957:Plasmids
D003062:Codon; D009838:Oligodeoxyribonucleotides