title: Lyophilized airborne Clostridium botulinum spores as inocula that intestinally colonize antimicrobially pretreated adult mice.
abstract: Adult mice, made susceptible to Clostridium botulinum by feedings of metronidazole, were immobilized with an anesthetic and held for 30 min in isolators in which a fine powder of lyophilized pathogen spores was made airborne. Exposed mice were surface decontaminated before being kept for 2 days in holding isolators. Mice were intestinally colonized by the pathogen. Colonization rates were related to spore numbers (10(4) to 10(7) type A or B) seeded into isolators.
mesh_terms: D000336:Aerosols; D000818:Animals; D001906:Botulism / Q000635:transmission*; D003014:Clostridium botulinum / Q000472:pathogenicity*; D005612:Freeze Drying; D008795:Metronidazole / Q000494:pharmacology; D051379:Mice; D013171:Spores, Bacterial
chemical_list: D000336:Aerosols; D008795:Metronidazole