Fluorescent antibody technique to detect Cowdria ruminantium in in vitro-cultured macrophages and buffy coats from cattle, sheep, and goats.
Fluorescent antibody tests, Giemsa stain, and electron microscopy were used to detect colonies of Cowdria ruminantium in in vitro-cultured macrophages and buffy coats from heartwater-infected cattle, sheep, and goats. Antibodies were obtained from C ruminantium-infected cattle, sheep, and goats treated with a small dose of oxytetracycline HCl. Cowdria ruminantium elementary bodies were small-coccus forms (0.14 micron) and large-coccus forms (0.22 micron to 0.6 micron). The size of inclusion bodies varied from 1.5 micron to 2 micron. Inclusion bodies and elementary bodies were observed in the cytoplasm of macrophages and neutrophils.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001773:Blood Cells / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000382:microbiology*; D006357:Heartwater Disease / Q000382:microbiology*; D008264:Macrophages / Q000382:microbiology*; D012287:Rickettsiaceae / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
D000942:Antigens, Bacterial