Modulation of murine coxsackievirus-induced myocarditis utilizing anti-idiotypes.
Pre-treatment of syngeneic mice with polyclonal populations of Protein-A separated anti-idiotypic antibodies prepared against anti-CVB3 viral idiotypes resulted in reduction of inflammatory myocarditis in virus-challenged mice. Cellular immunity as assessed by cell-migration-inhibition resulted in specific cell-mediated sensitivity against anti-Ids (CVB3) as well as viral preparations. Animals pre-treated with anti-idiotypic preparations developed an anamnestic anti-viral antibody response, with antibodies capable of specifically binding CVB3 virus antigen in an ELISA assay; but without CVB3 viral neutralizing capability. Adoptive transfer of limited numbers of syngeneic anti-Id immunized lymphoid cell populations failed to alter the course of inflammatory myocarditis in CVB3 virus-challenged animals. Cellular binding studies utilizing anti-Ids suggested increased, but nonspecific binding of anti-Ids to lymphoid and myocyte populations in CVB3 infected animals. The data suggest an immunomodulatory role of idiotype-anti-idiotype interactions in the development of myocarditis.
D000818:Animals; D000917:Antibody Formation; D000954:Antigens, Surface / Q000032:analysis; D002464:Cell Migration Inhibition; D002846:Chromatography, Affinity; D003384:Coxsackievirus Infections / Q000276:immunology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D007111:Immunity, Cellular; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009205:Myocarditis / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage*
D000954:Antigens, Surface; D007130:Immunoglobulin Idiotypes; D014765:Viral Vaccines