Plasmodium falciparum polypeptides associated with the infected erythrocyte plasma membrane.
Plasmodium falciparum proteins associated with plasma membranes of infected erythrocytes were identified by using three techniques: isolated plasma membranes from infected and uninfected erythrocytes were compared by gel electrophoresis and silver staining; isolated plasma membranes from cells metabolically labeled with [35S]methionine were assayed by gel electrophoresis; and uninfected and infected intact erythrocytes were surface-labeled by lactoperoxidase iodination, and the labeled polypeptides were compared by gel electrophoresis. The results from these experiments indicate that at least six parasite-derived polypeptides (Mr = greater than 240,000, 150,000, 55,000, 45,000, 35,000, and 20,000) are associated with the infected erythrocyte plasma membrane. At least four of these peptides (Mr = 55,000, 45,000, 35,000, and 20,000) may be exposed on the surface of the infected erythrocytes.
D000818:Animals; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D008288:Malaria / Q000097:blood / Q000276:immunology*; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology* / Q000648:ultrastructure
D010455:Peptides