A recombinant IGF-1 analogue stimulates [3H]thymidine incorporation in human epiphyseal growth cartilage in vitro.
The effect of a recombinant analogue of IGF-1 on human cartilage of different ages has been investigated in vitro. Addition of 100 ng/ml IGF-1 to articular and epiphyseal growth plate cartilage (9 months to 16 years) resulted in an increase in [3H]thymidine incorporation to a mean of 130 +/- 4% (SE) and 378 +/- 53% of controls, respectively. In costal and growth cartilage from normal fetuses of 15-18 weeks gestation, [3H]thymidine incorporation was increased to 119 +/- 3 and 133 +/- 11% of controls, respectively, when 100 ng/ml IGF-1 was added. The mitogenic effect of recombinant IGF-1 in vitro extends to human cartilage and is most notable in postnatal epiphyseal growth plate.
D000293:Adolescent; D000367:Age Factors; D002675:Child, Preschool; D005260:Female; D005333:Fetus / Q000378:metabolism; D006132:Growth Plate / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007334:Insulin-Like Growth Factor I / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D008934:Mitogens; D013002:Somatomedins / Q000031:analogs & derivatives*; D013936:Thymidine / Q000378:metabolism
D008934:Mitogens; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D013936:Thymidine