5,6-Epoxyeicosatrienoic acid mobilizes Ca2+ in anterior pituitary cells.
Luteinizing hormone releasing hormone stimulates the concomitant release of luteinizing hormone and 45Ca2+ from prelabeled anterior pituitary cells. Indomethacin (10 microM) and nordihydroguaiaretic acid (10 microM) had no effect on the luteinizing hormone releasing hormone-stimulated release of either luteinizing hormone or 45Ca2+. Eicosatetraynoic acid (10 microM) blocked both luteinizing hormone releasing hormone-stimulated luteinizing hormone secretion and luteinizing hormone releasing hormone-stimulated 45Ca2+ efflux. 5,6-Epoxyeicosatrienoic acid stimulated both luteinizing hormone secretion and 45Ca2+ efflux from anterior pituitary cells. Additionally, 5,6-epoxyeicosatrienoic acid closely mimics the ability of luteinizing hormone releasing hormone to increase intracellular free calcium. These results are consistent with the hypothesis that 5,6-EET alters calcium homeostasis in a manner similar to that observed during luteinizing hormone releasing hormone stimulation of luteinizing hormone release.
D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004987:Ethers / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D015759:Ionomycin; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009637:Masoprocol / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004987:Ethers; D005231:Fatty Acids, Unsaturated; D007987:Gonadotropin-Releasing Hormone; D015759:Ionomycin; D009637:Masoprocol; C040776:5,6-epoxy-8,11,14-eicosatrienoic acid; D007986:Luteinizing Hormone; D015126:8,11,14-Eicosatrienoic Acid; D002118:Calcium; D007213:Indomethacin