Effects of retinoic acid on growth, insulin secretion, and hexose incorporation into macromolecules of a continuously growing, insulin-secreting cell line (RINm5F).
The continuously growing, insulin-secreting cell line RINm5F does not respond to glucose with increased rates of insulin secretion and cell proliferation. The possibility that retinoic acid, which acts as a differentiating agent in several cell systems, could induce such responses to glucose has been investigated. Retinoic acid (10(-6)-10(-5) mol/l) failed to affect the cell viability, cell proliferation, 3H-thymidine incorporation or the DNA contents of the cultured RINm5F cells, irrespective of the glucose concentration of the culture medium. The insulin release was not affected either by glucose or by retinoic acid. Higher concentrations of the drug (10(-4) mol/l) proved toxic to the cells. The incorporation of 3H-mannose and 3H-glucosamine into TCA precipitable material of the RINm5F cells was strongly decreased by an increased glucose concentration of the medium. The incorporation of 3H-mannose, but not that of 3H-glucosamine, into macromolecules which could be precipitated with Concanavalin A or wheat germ lectin was diminished by retinoic acid (10(-5) mol/l).
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014316:Tritium; D014909:Wheat Germ Agglutinins
D007328:Insulin; D037102:Lectins; D014909:Wheat Germ Agglutinins; D014316:Tritium; D003208:Concanavalin A; D014212:Tretinoin; D008358:Mannose; D013936:Thymidine