Identity between cytoplasmic and membrane-bound S-100 proteins purified from bovine and rat brain.
Cytoplasmic and membrane-bound S-100 proteins were purified to homogeneity from bovine and rat brain. Cytoplasmic and membrane-bound S-100 from single species are identical by immunological, electrophoretic, spectrophotometric, and functional criteria. Cytoplasmic and membrane-bound S-100 from bovine brain consists of nearly equal amounts of S-100a and S-100b, whereas cytoplasmic and membrane-bound S-100 from rat brain consists mostly of S-100b. The functional role of membrane-bound S-100 remains to be elucidated.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis*; D002845:Chromatography; D003593:Cytoplasm / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D007700:Kinetics; D051381:Rats; D009418:S100 Proteins / Q000032:analysis*; D013053:Spectrophotometry
D009418:S100 Proteins