Selective complement fixation by pancreatic B-cells after binding to islet cell surface antibodies.
A two wavelength immunofluorescence system was used to demonstrate islet cell surface antibodies (ICSA) from juvenile diabetics and complement on the same viable rat islet cell. When incubated with rat islet cells at 4 C and at 37 C, ICSA specific for B-cells proved capable of binding complement. In contrast, ICSA specific for both B and non-B cells were able to fix complement on all cell types at 4 C but only on B-cells at 37 C. This study provides further evidence for the B-cell specific cytotoxic effect of ICSA in the presence of complement at physiological temperature. In addition, the ultrastructural events leading to B-cell destruction after ICSA and complement binding were studied by transmission electron microscopy (TEM).
D000818:Animals; D001323:Autoantibodies / Q000378:metabolism*; D001666:Binding Sites, Antibody; D003179:Complement C3b / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D001323:Autoantibodies; C024211:islet cell antibody; D003179:Complement C3b