[Selective method of isolating mutants sensitive to ultraviolet radiation from a culture of Rhodopseudomonas sphaeroides].
The method of penicillin selection used after UV-irradiation (lambda = 254 nm) allows one to select UV-sensitive mutants (uvs-mutants) of the phototrophous bacterium Rhodopseudomonas sphaeroides induced by nitrosomethylurea with an effectiveness greater by an order of magnitude. Over 30% of the uvs-mutants obtained using this method had an elevated sensitivity not only to far-UV (F-UV, lambda = 254 nm) but also to near-UV (N-UV, lambda greater than 280 nm) UV-irradiation. No correlation was found in the degree of sensitivity to F-UV and N-UV-irradiation of the uvs-mutants. Mutants highly sensitive to the lethal action of N-UV were isolated.
D005821:Genetic Techniques; D008770:Methylnitrosourea / Q000494:pharmacology; D009154:Mutation; D010406:Penicillins / Q000494:pharmacology; D011836:Radiation Tolerance; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000302:isolation & purification / Q000528:radiation effects*; D012641:Selection, Genetic; D014466:Ultraviolet Rays
D010406:Penicillins; D008770:Methylnitrosourea
Selektivnyĭ metod vydeleniia mutantov, chuvstvitel'nykh k ul'trafioletovomu izlucheniiu, kul'tury Rhodopseudomonas sphaeroides.