[Culture systems for production of promastigote and amastigote forms of Leishmania. Application to serological diagnosis and therapeutic trials].
Several species of leishmania and three methods of cultivation: monophasic, biphasic and co-cultivation were used in a compared study bearing on the intensive production of leishmania. In addition by applying, a new in vivo model, comprising an injection of sarcomatous cells and promastigotes into BALB/c mice and also an extraction on a discontinuous gradient (Radioselectan 60%), it was possible to obtain highly purified isolates of amastigote forms. The use of two antigens: promastigotes and amastigotes, is to be recommended for the serological diagnosis, by indirect immunofluorescence, of kala-azar. The new in vivo model merits further consideration for research concerning new molecules active against leishmania.
D000818:Animals; D000965:Antimony / Q000627:therapeutic use*; D000981:Antiprotozoal Agents / Q000627:therapeutic use*; D002460:Cell Line; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D007891:Leishmania / Q000254:growth & development*; D007896:Leishmaniasis / Q000188:drug therapy; D008536:Meglumine; D000077485:Meglumine Antimoniate; D051379:Mice; D008807:Mice, Inbred BALB C; D009942:Organometallic Compounds; D012698:Serologic Tests; D013997:Time Factors
D000981:Antiprotozoal Agents; D003470:Culture Media; D009942:Organometallic Compounds; D008536:Meglumine; D000077485:Meglumine Antimoniate; D000965:Antimony
Etude de systèmes de culture pour la production des formes promastigotes et amastigotes des Leishmanies. Application au diagnostic sérologique et aux essais thérapeutiques.