title: Recognition of a granulocyte-specific membrane protein by monoclonal antibodies.
abstract: Using membranes isolated from leukemic myeloblasts as immunogen, two granulocyte specific monoclonal antibodies 38D2 and 41D2 were developed. The antibodies are positive in membrane immunofluorescence with granulocytes and bands; they give no reaction with monocytes, platelets, leukemic myeloblasts, lymphoblasts, myeloid cell lines, and immature myeloid cells from bone marrow. Both monoclonal antibodies precipitate the same membrane glycoprotein (MW: 70-78 kd, pI: 6-7), which corresponds to a membrane glycoprotein characteristic for granulocytes, as concluded from two-dimensional polyacrylamide gelelectrophoresis patterns obtained from surface labelled myeloid cells. The monoclonal antibodies did not influence the granulocyte functions tested: phagocytosis of opsonized bacteria, chemiluminiscence after stimulation with various stimuli, and chemotaxis.
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000032:analysis; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D010587:Phagocytosis
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D008565:Membrane Proteins