[Isolation and characterization of erythrogenic toxins. VIII. Purification of a biologically active protein of the molecular weight 10,000 (LMP-10k) from filtrates of Streptococcus pyogenes, strain NY-5. Relationship to erythrogenic toxin type A].
The "classical" method for purification of erythrogenic toxin type A results in two products: erythrogenic toxin type A and a low molecular weight protein, m.w. 10,000 (LMP-10k) with mitogenic activities. LMP-10k was purified from culture supernatants of S. pyogenes (group A) by CM-Sepharose CL-6B chromatography and Sephacryl S-200 and Sephadex G75 gel filtration to a high degree of purity with minimal amounts of residual erythrogenic toxin A. The isoelectric point of LMP-10k is the same as for erythrogenic toxin A: 5.2. The immunogenic activity is low, only one of two rabbits produced anti-LMP-10k-antibodies after a prolonged course of immunization. On the other hand it is possible to induce antierythrogenic toxin A-antibodies by immunization with LMP-10k preparations contaminated with small amounts of erythrogenic toxin A. Possibly the data given by some authors for the m.w. of erythrogenic toxin type A as 8 000 D are the results of a mix-up with co-purified LMP-10k.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005098:Exotoxins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008565:Membrane Proteins; D008970:Molecular Weight; D011817:Rabbits; D013297:Streptococcus pyogenes / Q000378:metabolism*
D000596:Amino Acids; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin
Isolierung und Charakterisierung von erythrogenen Toxinen VIII. Die Reinigung eines biologisch aktiven Proteins vom Molekulargewicht 10 000 (LMP-10k) aus Kulturfiltraten des Streptococcus pyogenes, Stamm NY-5. Beziehung zum erythrogenen Toxin Typ A.