Immunoreactive thymopoietin in the mouse central nervous system.
A thymopoietin-immunoreactive substance (TP-IRS) has been detected in homogenates of mouse spinal cord and brain using a radioimmunoassay; levels were maximal at birth. TP-IRS was also detected in supernatants of mouse neuroblastoma (NIE-115) and primary spinal cord cultures but not human astrocytic and meningeal tumors or mouse primary astrocyte cultures. With affinity purified rabbit anti-TP globulin, immunofluorescent staining was seen in mouse spinal cord cultures in association with nuclear membranes of neurons and, to a lesser degree, flat background cells. From supernatants of NIE-115 cells grown in tritiated leucine and lysine, proteins of approximately 8000 and 4500 Da were isolated by TP affinity chromatography (compared with 5562 Da for thymic thymopoietin). When injected into mice, these neural proteins partially blocked neuromuscular transmission in a manner similar to thymic thymopoietin.
D000818:Animals; D001253:Astrocytes / Q000032:analysis; D002460:Cell Line; D002490:Central Nervous System / Q000032:analysis*; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000032:analysis; D006801:Humans; D007525:Isoelectric Focusing; D008579:Meningioma / Q000032:analysis; D051379:Mice; D009447:Neuroblastoma; D011863:Radioimmunoassay; D051381:Rats; D013946:Thymopoietins / Q000032:analysis*; D013951:Thymus Hormones / Q000032:analysis*
D013946:Thymopoietins; D013951:Thymus Hormones