Characterization of an antigen from rat male accessory glands.
An isolated soluble antigen involved in the autoimmune response against rat male accessory glands migrated as a double band when it was submitted to analytical disc polyacrylamide gel electrophoresis and as a homogeneous molecular species in SDS electrophoresis. The purified antigen had a molecular weight of approximately 78 K by SDS electrophoresis and 80 K by gel filtration chromatography. The antigen was identified as a protein and displays androgen dependency. The purified fraction was pathogenically active in microgram doses and induced humoral and delayed-type hypersensitivity responses. Besides, lesions were observed in the target organ. The cellular infiltration was located in prostate glands according to the localization of antigen by immunofluorescence findings.
D000367:Age Factors; D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D001324:Autoantigens / Q000276:immunology / Q000302:isolation & purification*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005837:Genitalia, Male / Q000276:immunology*; D007114:Immunization; D008297:Male; D008970:Molecular Weight; D009919:Orchiectomy; D011467:Prostate / Q000276:immunology; D011506:Proteins / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012669:Seminal Vesicles / Q000276:immunology; D012741:Sexual Maturation
D000941:Antigens; D001324:Autoantigens; D011506:Proteins