Identification and characterization of the protein encoded by the human c-myb proto-oncogene.
We have identified the product of the human c-myb proto-oncogene as a 80,000-Mr protein, p80c-myb, by using polyclonal and monoclonal antibodies raised against a bacterially synthesized polypeptide from the amino terminus of the viral myb protein. p80c-myb shares at least two distinct antigenic sites with the amino terminal region of the v-myb protein. p80c-myb is found only in hematopoietic cells or in cells that contain amplified c-myb genes. Like the chicken myb proteins, p80c-myb is a nuclear DNA-binding protein that is predominantly associated with chromatin and exhibits a short half-life of approximately 1 hour.
D000818:Animals; D000911:Antibodies, Monoclonal; D009189:Avian Myeloblastosis Virus / Q000235:genetics; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D003110:Colonic Neoplasms; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005822:Genetic Vectors; D006207:Half-Life; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000302:isolation & purification; D020598:Proto-Oncogene Proteins c-myb; D011519:Proto-Oncogenes; D011817:Rabbits
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb