[Effect of nutrient medium perfusion on the secretory activity of rat hepatocytes and pancreatic islet cells].
The data are reported on albumin secretion by rat hepatocytes and insulin secretion by pancreatic beta-cells of newborn rats during cell cultivation on flat synthetic membrane in conditions of continuous medium perfusion. Albumin and insulin secretion by the appropriate cultures was higher in continuous medium perfusion than in the control. Enhanced sensitivity of pancreatic beta-cells to glucose, as compared to the control was revealed. It is concluded that continuous medium perfusion of hepatocytes and pancreatic beta-cells in the primary culture had a favourable effect on albumin and insulin secretion by the appropriate cultures.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D003470:Culture Media / Q000008:administration & dosage; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D010477:Perfusion; D051381:Rats
D000418:Albumins; D003470:Culture Media; D007328:Insulin
Vliianie perfuzii pitatel'noĭ sredoĭ na sekretornuiu aktivnost' gepatotsitov i ostrovkovykh kletok podzheludochnoĭ zhelezy krys.