title: Assembly and dynamics of the actin filament system in nonmuscle cells.
abstract: Kinetic analysis has provided a detailed quantitative description of the mechanism of actin polymerization as well as the methods to analyze the mechanisms of action of actin-binding proteins. In Acanthamoeba, five different proteins regulate the pool of monomers available for polymerization, cap the end of filaments, sever filaments, and cross-link filaments. Remarkably, many of these interactions involve very-low-affinity bonds between the protein molecules.
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000656:Amoeba / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D018260:Gelsolin; D007700:Kinetics; D046911:Macromolecular Substances; D008274:Magnesium / Q000378:metabolism; D008840:Microfilament Proteins; D011487:Protein Conformation
chemical_list: D000199:Actins; D002352:Carrier Proteins; D018260:Gelsolin; D046911:Macromolecular Substances; D008840:Microfilament Proteins; C016953:brevin; D008274:Magnesium; D002118:Calcium