Mutant of Salmonella typhimurium LT2 deficient in DNA adenine methylation.
A mutant of Salmonella typhimurium LT2 deficient in methylation of the adenine residues in the sequence 5'-GATC-3' was isolated. The mutation (dam-1) was linked to the cysG locus, and the properties of the mutant were similar to those of Escherichia coli dam mutants. Reversion of the hisC3076 frameshift marker by 9-aminoacridine was substantially enhanced by the dam-1 mutation, implying a direct role for adenine methylation in the prevention of frameshift mutation induction.
D000225:Adenine / Q000378:metabolism*; D000585:Aminacrine / Q000494:pharmacology; D004269:DNA, Bacterial / Q000378:metabolism*; D005798:Genes, Bacterial; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
D004269:DNA, Bacterial; D000585:Aminacrine; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D000225:Adenine