Luminescent visualization of antigens on blots.
Proteins separated by SDS gel electrophoresis and transferred to a nitrocellulose sheet can be visualised by 'probing' with peroxidase-linked reagents which are detected by luminescence. A modified luminescent substrate is described containing 4-methylumbelliferone which enhances light emission four-fold. Using the modified substrate, luminescent detection was found to be more sensitive than chromogenic detection of peroxidase using 4-chloro-1-napthol. The new technique was used in conjunction with the immunoblot method to demonstrate antigenic differences between rat and mouse erythrocytes.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D002863:Chromogenic Compounds; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane; D006923:Hymecromone / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D051381:Rats
D000941:Antigens; D002863:Chromogenic Compounds; D008565:Membrane Proteins; D006923:Hymecromone; D003101:Collodion