Regeneration of T lymphocytes from human bone marrow cells after depletion with methylprednisolone.
This study evaluates the propagation of T lymphocytes in bone marrow cells after in vitro methylprednisolone treatment. Previous studies from this laboratory showed that immunocompetent T lymphocytes could be eliminated from the bone marrow cells by incubation with 15 mg/ml (0.04 M) of methylprednisolone for 1 hr. The effectiveness of the chemoseparation was assessed by E-rosette forming cell assay, mitogen-induced lymphoblastogenic responses, and lymphocyte surface markers. In this study, bone marrow cells treated with different concentrations of methylprednisolone were cultured in the presence of PHA and Interleukin-2. The mitogen-induced lymphoblastogenesis was restored in the 15 mg/ml MP-treated bone marrow cells by the 8th day of culture in the presence of Interleukin-2. The cells in the culture were analyzed by E-rosette forming cell assay. It was shown that the predominant cells in the cultures were E-rosette forming cells. This study demonstrates that immunocompetent T lymphocytes can be regenerated in the presence of mitogen and Interleukin-2 from bone marrow cells treated with 15 mg/ml of methylprednisolone.
D001853:Bone Marrow / Q000187:drug effects; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D006087:Graft vs Host Reaction / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000008:administration & dosage; D008213:Lymphocyte Activation / Q000187:drug effects; D008212:Lymphocyte Depletion; D008775:Methylprednisolone / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000008:administration & dosage; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects*; D014184:Transplantation, Homologous
D007376:Interleukin-2; D010835:Phytohemagglutinins; D008775:Methylprednisolone