A new ganglioside in human meconium detected with antiserum against human milk sialyltetrasaccharide a.
Antiserum directed against the alditol derivative of the human milk monosialyloligosaccharide sialyltetrasaccharide a [D. F. Smith, P. A. Prieto, and B. V. Torres (1985) Arch. Biochem. Biophys. 241, 298-303] is used to detect a new ganglioside in human meconium by direct binding on nitrocellulose filters of the sialyl[3H]oligosaccharide alditol obtained from gangliosides after ozonolysis and alkali fragmentation. The sialyl[3H]oligosaccharide is purified by affinity chromatography on a column containing anti-sialyltetrasaccharide a antibodies. The affinity-purified sialyl[3H]oligosaccharide cochromatographs with the 3H-labeled alditol derivative of authentic sialyltetrasaccharide a from human milk. Results of sequential enzyme degradation of the pure sialyl[3H]oligosaccharide and cochromatography of the digestion products with standards are consistent with the presence in meconium of a monosialylganglioside with the structure NeuAc alpha 2-3Gal beta 1-3GlcNAc beta 1-3Gal beta 1-4Glc-ceramide. This ganglioside is presumably the biosynthetic precursor of the sialyl-Lea ganglioside [G. C. Hansson and D. Zopf (1985) J. Biol. Chem. 260, 9388-9392], which is also a component of human meconium.
D002846:Chromatography, Affinity; D005260:Female; D005732:Gangliosides / Q000032:analysis*; D006801:Humans; D007158:Immunologic Techniques; D007231:Infant, Newborn; D008470:Meconium / Q000032:analysis*; D008895:Milk, Human / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis* / Q000276:immunology
D005732:Gangliosides; D009844:Oligosaccharides; C016735:sialooligosaccharides