Partial purification of porcine platelet-derived growth factor (PDGF).
Fresh porcine blood platelets, subjected to ethanol acid extraction and purification on DEAE-Sephadex A-50 and CM-Sephadex C-50, were used to obtain partially purified, cationic PDGF (pI 8.5-10) of Mr 38,000 and biological activity 10(4) U/ml. Reduction of porcine PDGF with 2-mercaptoethanol revealed the presence of two polypeptides with Mr 18,000-22,000.
D000818:Animals; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D005347:Fibroblasts / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010982:Platelet-Derived Growth Factor / Q000302:isolation & purification* / Q000502:physiology; D013552:Swine
D010982:Platelet-Derived Growth Factor