[A simple enzyme assay for the simultaneous determination of penicillin derivatives and clavulanic acid in biological fluids].
A simple enzymatic assay for simultaneous determinations of amino-/ureidopenicillins and clavulanic acid concentrations in various body fluids is described; no pretreatment of the samples is required. The assay is based on the competitive inhibition of the chromogenic compound Padac by clavulanic acid in the presence of Proteus vulgaris beta-lactamase. Detection limit for clavulanic acid amounts 0.02 mg/l. Interferences with other antibiotics such as aminoglycosides, macrolides, quinolones etc. can be ruled out.
D001667:Binding, Competitive; D002511:Cephalosporins / Q000494:pharmacology; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D004796:Clinical Enzyme Tests / Q000379:methods*; D006801:Humans; D010406:Penicillins / Q000032:analysis* / Q000097:blood / Q000652:urine; D011514:Proteus vulgaris / Q000201:enzymology; D001618:beta-Lactamases / Q000494:pharmacology
D002511:Cephalosporins; D002969:Clavulanic Acids; D010406:Penicillins; D019818:Clavulanic Acid; C034313:pyridine-2-azo-4-dimethylaniline cephalosporin; D001618:beta-Lactamases
Ein einfaches enzymatisches Verfahren zur simultanen Bestimmung von Penicillinderivaten und Clavulansäure in biologischen Flüssigkeiten.