A simple and generally applicable procedure for releasing DNA from bacterial cells.
Treatment of Staphylococcus simulans biovar staphylolyticus cells with acetone before digestion with lysozyme made the cells susceptible to lysis by sodium dodecyl sulfate. This technique was found to be useful for releasing DNA from a wide variety of gram-positive and gram-negative organisms.
D000096:Acetone / Q000494:pharmacology; D001431:Bacteriological Techniques; D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D009113:Muramidase / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*
D004269:DNA, Bacterial; D000096:Acetone; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase