The 52-kDa estrogen-induced protein secreted by MCF7 cells is a lysosomal acidic protease.
An estrogen-induced 52-kDa glycoprotein secreted by human breast cancer cells and able to autostimulate the growth of MCF7 cells has been purified, using monoclonal antibodies, and characterized. The protein contains mannose 6-phosphate signals on its N-linked high-mannose chains, suggesting that it is a lysosomal enzyme. Both the secreted 52-kDa protein and its processed cellular forms (52-, 48- and 34-kDa) were identified as carboxyl proteinases having an optimal activity at pH 3.5 and being specifically inhibited by pepstatin. This protease is characterized by its inducibility by estrogens and its high concentration in proliferative benign and malignant mammary tissue, when detected by immunohistochemistry. The estrogen-induced secretion of this protease may help to understand how estrogens stimulate mammary tumor growth and/or invasion.
D000911:Antibodies, Monoclonal; D016282:Aspartic Acid Endopeptidases; D001943:Breast Neoplasms / Q000201:enzymology*; D002402:Cathepsin D / Q000378:metabolism; D002460:Cell Line; D004590:Electrophoresis, Paper; D010450:Endopeptidases / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D008360:Mannosephosphates / Q000032:analysis; D008970:Molecular Weight; D010436:Pepstatins / Q000494:pharmacology; D011480:Protease Inhibitors
D000911:Antibodies, Monoclonal; D004967:Estrogens; D008360:Mannosephosphates; D010436:Pepstatins; D011480:Protease Inhibitors; C014195:Streptomyces pepsin inhibitor; C027693:mannose-6-phosphate; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; D002402:Cathepsin D; C031375:pepstatin