Changes in the nucleoprotein complexes of a baculovirus DNA during infection.
The nature of the DNA-protein complexes assumed by Autographa californica nuclear polyhedrosis virus (AcNPV) DNA during infection of Spodoptera frugiperda cells was investigated by micrococcal nuclease digestion of infected nuclei. Both parental viral DNA and progeny viral DNA assumed a chromatin-like structure early in infection. By late times (24 hr) p.i., the viral DNA acquired a unique nucleoprotein structure. In addition to fragments of mononucleosome size (185 bp), two subnucleosomal bands of 120 and 90 bp were observed. The subnucleosomal bands contained exclusively viral DNA. No alteration in the nature of the host chromatin structure following AcNPV infection was observed. An examination of the basic chromatin-associated proteins revealed two major viral-induced proteins having molecular weights of 15K and 39K. The induction of the basic 15K protein between 10 and 24 hr p.i. coincided with the appearance of the altered nucleoprotein structure observed by 24 hr p.i. and the cessation of histone synthesis.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis; D004279:DNA, Viral / Q000378:metabolism*; D006657:Histones / Q000096:biosynthesis; D007304:Insect Viruses / Q000254:growth & development / Q000378:metabolism*; D007915:Lepidoptera; D008836:Micrococcal Nuclease / Q000378:metabolism; D008970:Molecular Weight; D009698:Nucleoproteins / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism*
D002843:Chromatin; D004279:DNA, Viral; D006657:Histones; D009698:Nucleoproteins; D009707:Nucleosomes; D014764:Viral Proteins; D008836:Micrococcal Nuclease