title: Myocardial norepinephrine, epinephrine and dopamine concentrations after cardiac autotransplantation in dogs.
abstract: Myocardial norepinephrine is markedly reduced after cardiac transplantation because of interruption of postganglionic cardiac sympathetic nerves. There are also substantial stores of dopamine in the myocardium, but the influence of cardiac denervation on dopamine remains unknown. The effect of cardiac transplantation was determined and, thus, the effect of denervation on myocardial norepinephrine, dopamine and epinephrine. Myocardial catecholamines were measured with high-performance liquid chromatography with electrochemical detection in five dogs 6 to 8 weeks and in four dogs 8 to 12 years after cardiac autotransplantation and in six sham-operated dogs with intact cardiac innervation. Norepinephrine, dopamine and epinephrine levels were determined from samples obtained from the right and left atria and ventricles. Samples from the left ventricular apex and base were analyzed separately. There was a striking depletion of norepinephrine in all cardiac chambers after short-term autotransplantation. The norepinephrine content of the left atrium in sham-operated dogs (1,659 +/- 219 ng/g) was significantly higher than that of dogs with long-term autotransplanted hearts (754 +/- 372 ng/g). Sham-operated dogs and dogs with long-term autotransplanted hearts had statistically significant (p less than 0.05) differences in norepinephrine content in the left ventricular apex (480 +/- 197 versus 294 +/- 198 ng/g), left ventricular base (876 +/- 2204 versus 654 +/- 156 ng/g) and right ventricle (766 +/- 133 versus 247 +/- 29 ng/g). In contrast to norepinephrine, dopamine concentrations were relatively preserved in the short-term group despite the virtual depletion of myocardial norepinephrine.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000537:Aluminum Oxide; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004298:Dopamine / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D006321:Heart / Q000294:innervation; D016027:Heart Transplantation; D009206:Myocardium / Q000378:metabolism*; D009416:Nerve Regeneration; D009638:Norepinephrine / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000502:physiology; D013997:Time Factors
chemical_list: D002395:Catecholamines; D000537:Aluminum Oxide; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine