Isolation and localization of filamin in heart muscle.
High-molecular-mass protein was isolated from chicken heart muscle. The apparent molecular mass of a single polypeptide chain is similar to that of chicken gizzard filamin: 250-270 kDa. The protein interacts with antibodies against chicken gizzard filamin and induces F-actin gelation in a concentration-dependent manner. Immunofluorescent staining of cardiomyocytes and chicken heart sections with antifilamin antibody demonstrates two types of filamin localization: filamin was located on the sarcomere border in the periphery of the Z-disk; filamin was found in intercalated disks between cardiomyocytes.
D000818:Animals; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D003285:Contractile Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D064448:Filamins; D005455:Fluorescent Antibody Technique; D005895:Gizzard, Avian; D008840:Microfilament Proteins / Q000302:isolation & purification*; D008858:Microscopy, Phase-Contrast; D009206:Myocardium / Q000032:analysis* / Q000648:ultrastructure; D009210:Myofibrils / Q000032:analysis / Q000648:ultrastructure
D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins