Matrix models. Essential tools for microscopic cytochemical research.
An overview is given of the preparation and use of model systems for cytochemistry, dealing with quantitative as well as qualitative aspects. Descriptions are given of the various possibilities to prepare cytochemical matrix models, ranging from macroscopic and microscopic films, to models with more cell-like dimensions as agarose beads, artificial cells and erythrocyte ghosts. Such models allow the study of a large variety of cytochemical processes. Their potentialities are demonstrated in a number of specific applications, comprising: the study of the influence of fixation on cellular processes, reaction specificity and reaction kinetics, quality of reagents and biochemical calibration in cytochemical staining; factors influencing localization of the specific endproduct in enzyme cytochemistry; immunocytochemistry and hybridocytochemistry.
D000180:Acrylic Resins; D000818:Animals; D001219:Aspartate Aminotransferases; D002451:Cell Compartmentation; D002482:Cellulose; D003124:Colorimetry / Q000379:methods; D003593:Cytoplasm / Q000648:ultrastructure*; D004910:Erythrocyte Membrane; D005404:Fixatives; D005898:Glass; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D009693:Nucleic Acid Hybridization; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001219:Aspartate Aminotransferases; D002482:Cellulose; D005404:Fixatives; D000099166:Polyacrylamides