The regulatory role of sialic acids in the response of class II reactive T cell hybridomas to allogeneic B cells.
Two different kinds of alloreactive T cell hybridomas were established in previous experiments. One is reactive and the other is nonreactive to allogeneic I-A region-associated membrane antigen (mIa) on B cells. In the present experiments the difference between these hybridomas were analyzed by using representative clones, B cell mIa-reactive clone CB-11.4, and nonreactive clone HTB-9.3. Unresponsiveness of HTB-9.3 clone to allogeneic B cells could not be due to the inability of B cells in interleukin 1 production or the density of mIa molecules on B cells. HTB-9.3 clone could respond to C57BL/6 mouse B cells treated with neuraminidase (Nase), and Nase-treated HTB-9.3 clone could respond to normal B cells from C57BL/6 mouse, indicating that sialic acid on both B cells and HTB-9.3 clone plays a regulatory role in the alloreactivity of the clone. In response to B cells from C57BL/6 mouse, T cells from C3H/He mouse spleen showed similar reactivity to HTB-9.3 clone; that is, T cells could respond to Nase-treated B cells, and Nase-treated T cells to B cells, and T cells primed with C57BL/6 spleen cells in vitro showed similar reactivity to CB-11.4 clone. These results suggest that HTB-9.3 clone represents virgin T cells and CB-11.4 clone-primed T cells at least in alloreactivity. Anti-L3T4a was shown to block alloreactivities of both T cell hybridomas and splenic T cells against B cells more efficiently than against splenic adherent cells. These results suggest that L3T4a on T cell plays more important role in allogeneic response to B cells than to splenic adherent cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D007375:Interleukin-1 / Q000302:isolation & purification; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009439:Neuraminidase / Q000494:pharmacology*; D012794:Sialic Acids / Q000502:physiology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D007375:Interleukin-1; D012794:Sialic Acids; D009439:Neuraminidase