title: Immunohistochemical reactivity of monoclonal antibodies to human milk fat globule with breast carcinoma and with other normal and neoplastic tissues.
abstract: The immunohistochemical reactivity of three monoclonal antibodies, generated against human milk fat globule membranes (HMFG), were studied using peroxidase conjugated secondary antibody or avidin-biotin-peroxidase complex method. Two of the antibodies, III E 8 and III H 2 showed rather broad reactivity, staining all mammary, ovarian, endometrial, cervical and bronchial adenocarcinomas. They also stained all pancreatic adenocarcinomas but only 30% of the carcinomas of the colon. Reaction with prostatic and renal adenocarcinomas varied. No reaction was seen with squamous or transitional cell carcinomas. Antibody III D 5, which previously has been shown to recognize an estrogen receptor activation associated antigen in mammary carcinomas, stained 60% of mammary or gynecological adenocarcinomas. This antibody had a more limited reactivity with other tissues as well, as all intestinal carcinomas were negative. It stained all bronchial adenocarcinomas, however, and 50% of the prostatic carcinomas. The three monoclonal antibodies, although not specific, show a more restricted reactivity than the so far described HMFG antibodies and they may therefore be of value in histopathological diagnosis.
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D001940:Breast / Q000166:cytology; D001943:Breast Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008565:Membrane Proteins / Q000032:analysis*; D018396:Mucin-1; D009369:Neoplasms / Q000473:pathology*; D012016:Reference Values
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D000951:Antigens, Neoplasm; D008565:Membrane Proteins; D018396:Mucin-1