Induction of neoplastic lesions in the livers of C57BL x C3HF1 mice by chloral hydrate.
Chloral hydrate is a compound of environmental significance. The current investigation was undertaken to evaluate the carcinogenic effect of chloral hydrate, because it is present in drinking water and it is also used as a sedative. Fifteen-day-old C57BL x C3HF1 male mice were given a single dose of chloral hydrate in distilled water at two dose levels: group 1, 5 micrograms/g BW; group 2, 10 mu/g BW (20-25 mice per group). Thirty-five mice given distilled water only served as controls. Animals were sacrificed at 24 hr and thereafter at various intervals up to 92 weeks. The entire liver was fixed and examined histologically. Mice sacrificed between 48 and 92 weeks showed hepatic lesions ranging from hyperplastic to trabecular carcinomas. The tumor incidence in mice given 10 micrograms/g chloral hydrate (six of eight) was significantly higher (P less than 0.05) than the incidence in the controls (two of 19). These findings indicate that chloral hydrate should be more thoroughly studied for potential carcinogenicity.
D000818:Animals; D002697:Chloral Hydrate / Q000633:toxicity*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D008099:Liver / Q000473:pathology* / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008940:Mitotic Index / Q000187:drug effects
D002697:Chloral Hydrate