Small intestinal mucosal hyperplasia caused by an enterokinase inhibitor from red kidney bean in rats.
A specific enterokinase inhibitor (EKI) was purified from red kidney bean (RKB). Male weanling rats fed a diet containing this purified EKI (0.06%) for 6 d showed increases in mucosal weights, protein, DNA and lactic dehydrogenase contents in their small intestines compared to age-matched control rats fed a standard diet. Total mucosal EK and disaccharidase activities were, however, decreased in EKI-fed rats. Thus, oral consumption of EKI from RKB led to small intestinal mucosal hyperplasia in rats. The mucosal hyperplasia observed in EKI-fed rats is not likely due to decreased turnover of mucosal proteins as a result of reduced luminal proteases since luminal contents of trypsin, chymotrypsin and elastase in EKI-fed rats were similar to those of control rats. Enterokinase inhibitor may have a direct hyperplastic effect on the small intestine of rats.
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight; D010450:Endopeptidases; D004765:Enteropeptidase / Q000009:adverse effects / Q000037:antagonists & inhibitors*; D007887:Fabaceae / Q000009:adverse effects*; D006965:Hyperplasia / Q000209:etiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D010946:Plants, Medicinal; D011480:Protease Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin / Q000378:metabolism
D011480:Protease Inhibitors; D007770:L-Lactate Dehydrogenase; D010450:Endopeptidases; D014357:Trypsin; D004765:Enteropeptidase