Is phospholipid a required cofactor for the activity of mammalian signal peptidase?
To determine whether phospholipid is required for the activity of mammalian signal peptidase, the enzyme was partially purified from porcine pancreas and then extensively freed of phospholipid by SP-Sephadex C-50 chromatography. The delipidated enzyme showed signal peptidase activity, with a low concentration of detergent. Phospholipid was found to release the enzyme from the inhibition due to excess detergent.
D000818:Animals; D003902:Detergents / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D006358:Hot Temperature; D008565:Membrane Proteins; D010743:Phospholipids / Q000502:physiology*; D012697:Serine Endopeptidases; D013552:Swine
D003902:Detergents; D008565:Membrane Proteins; D010743:Phospholipids; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase