Immunohistologic identification of phenotypic antigens associated with Hodgkin and Reed-Sternberg cells. A paraffin section study.
A combination of two monoclonal antibodies, designated LN-1 and LN-2, were used in an attempt to identify the corresponding antigens in Hodgkin and Reed-Sternberg cells. The LN-1 antibody has been shown in previous studies in our laboratories to identify follicular center cells, whereas LN-2 marks certain B-cell subpopulations as well as interfollicular histiocytes. Utilizing the peroxidase-antiperoxidase (PAP) technique, paraffin-embedded sections were examined representing 39 cases of various histologic subgroups of Hodgkin's lymphoma following immunostaining with LN-1, LN-2, and the antibody to S-100. Of these 39 cases, the LN-2 antibody was found to consistently mark the majority of Hodgkin and Reed-Sternberg cells. LN-1 was found to identify Hodgkin and Reed-Sternberg cells in a smaller number of cases. In no instance were Hodgkin or Reed-Sternberg cells found to mark for the S-100 protein. The use of LN-1 and LN-2 antibodies facilitated the identification of Hodgkin and Reed-Sternberg cells and produced additional information regarding the phenotypic nature of these cells.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D006689:Hodgkin Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology; D010641:Phenotype; D009418:S100 Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D009418:S100 Proteins