The synthesis and use of oligodeoxynucleotides in plasmid DNA sequencing.
A convenient procedure for the synthesis and purification of oligonucleotides is described. 16-base long primers synthesised by this method were used to investigate DNA sequencing using plasmid DNA as a template. This allowed the further analysis of the E. coli glt A sequence coding for citrate synthase and enabled determination of the 5'-non-coding regulatory region of the aminoglycoside phosphotransferase gene.
D001483:Base Sequence; D003090:Coliphages; D004269:DNA, Bacterial; D004279:DNA, Viral; D004926:Escherichia coli; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D010957:Plasmids; D013698:Templates, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides