Bactericidal effects induced by laser irradiation and haematoporphyrin against gram-positive and gram-negative microorganisms.
Laser irradiation of tissues treated in vivo with haematoporphyrin (Hpr) is known to result in a cytocidal effect, reportedly more pronounced in tumour tissues. To ascertain whether this cytocidal phenomenon can occur not only in eukaryotic but also in prokaryotic cells, the authors devised a model system in vitro consisting of bacterial cultures in liquid and in solid media. Bacteria photosensitized with Hpr were subsequently exposed to laser beams and to daylight; then normal microbiological techniques were used to determine whether any bactericidal effect occurred. Satisfactory results were achieved, particularly against Gram-positive microorganisms; a partial inhibition of Gram-negative microorganisms was also observed.
D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects*; D006414:Hematoporphyrin Photoradiation; D007834:Lasers; D008027:Light; D010778:Photochemotherapy; D013211:Staphylococcus aureus / Q000187:drug effects*; D013997:Time Factors
D003470:Culture Media