Characterization of the T-cell proliferative response to a purified glycopeptide antigen (GP-25) present on the Trypanosoma cruzi cell surface.
A glycoconjugate (GP-25) was previously purified from Trypanosoma cruzi and shown to be a major immunogenic constituent of the parasite cell surface, capable of inducing specific humoral responses in the vast majority of patients with Chagas' disease. In the present study, the T-cell proliferative response to GP-25 was studied in mice immunized with T. cruzi fractions or whole parasites. Recognition of GP-25 by proliferating T cells requires the participation of syngeneic, accessory spleen cells and is specifically blocked by anti-la antibodies. Furthermore, recognition of GP-25 is influenced by the MHC haplotype of accessory antigen-presenting cells. Short-term, GP-25-specific T-cell lines were used to demonstrate the specificity of anti-GP-25 T cells and to show that this glycoconjugate is not involved in T-cell cross-reactivity with heart antigens. T cells primed with nonpathogenic trypanosomatids are able to recognize the purified T. cruzi GP-25 molecule, indicating that T cells recognize a GP-25 epitope which is shared among trypanosomatids.
D000818:Animals; D000918:Antibody Specificity; D000938:Antigen-Presenting Cells / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification; D006020:Glycopeptides / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007518:Isoantibodies / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D014345:Trypanosoma / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D006020:Glycopeptides; D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies