Characteristics of normal and maturity-onset diabetic (type II diabetes) cell cultures: life spans and DNA synthetic capabilities.
The replicative ability of fibroblasts derived from normal and type II diabetic (non-insulin-dependent) donors and their DNA synthetic capabilities in response to serum and insulin (+/- dexamethasone) have been studied. Comparative replicative life spans of the fibroblasts studied using several lots of serum showed no significant differences between the two donor groups with any lot of serum (p greater than 0.05). Insulin (i.e. 700 nM) and serum (10% v/v) stimulation of DNA synthesis in normal and type II diabetic cultures exhibited no differences in responses. The insulin:control ratios of the normal vs. type II diabetic were 1.61 +/- 0.08 vs. 1.81 +/- 0.11, respectively (p greater than 0.05) while the serum:control ratios were 3.55 +/- 0.58 vs. 4.02 +/- 0.54, respectively (p greater than 0.05). Dexamethasone amplification of the insulin-stimulated DNA synthetic response over a range of insulin concentrations (i.e. 1.6-66.6 nM) expressed no differences between the two donor groups. Additionally, calculation of the insulin concentration necessary for the half-maximal response showed no differences between the normal and diabetic groups (3.47 +/- 0.5 vs. 4.44 +/- 0.8 nM, respectively) (p greater than 0.05). The data suggest that there are no general age-related abnormalities inherent to the type II diabetic cultured cell.
D000328:Adult; D000368:Aged; D001769:Blood; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D003907:Dexamethasone / Q000494:pharmacology; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D013997:Time Factors
D007328:Insulin; D003907:Dexamethasone; D004247:DNA