The effects of 1-aminooxy-3-aminopropane and S-(5'-deoxy-5'-adenosyl)methylthioethylhydroxylamine on ornithine decarboxylase and S-adenosyl-L-methionine decarboxylase from Escherichia coli.
1-Aminooxy-3-aminopropane (APA) was shown to be a potent competitive inhibitor (Ki = 1.0 nM) of partially purified Escherichia coli ornithine decarboxylase. APA did not inhibit S-adenosyl-L-methionine decarboxylase and spermidine from E. coli. S-(5'-Deoxy-5'-adenosyl)methylthioethylhydroxylamine (AMA), which is a structural analogue of decarboxylated S-adenosyl-L-methionine, was for the first time shown to be an irreversible inhibitor of bacterial S-adenosyl-L-methionine decarboxylase and a competitive inhibitor (Ki = 47 microM) of bacterial ornithine decarboxylase. AMA had no effect on spermidine synthase.
D012437:Adenosylmethionine Decarboxylase / Q000037:antagonists & inhibitors*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D003839:Deoxyadenosines / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000201:enzymology*; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000096:biosynthesis*; D011437:Propylamines / Q000494:pharmacology*; D011436:Spermidine Synthase / Q000378:metabolism; D013997:Time Factors
D003839:Deoxyadenosines; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D011437:Propylamines; C042768:S-(5'-deoxyadenosyl-5')-S-methylthioethylhydroxylamine; C046252:1-aminooxy-3-aminopropane; D011436:Spermidine Synthase; D002262:Carboxy-Lyases; D012437:Adenosylmethionine Decarboxylase