[Relation between the changes in thiamine metabolism and energy processes in erythrocytes of patients with diabetes mellitus and approaches to their correction with drugs].
Erythrocytes, obtained from blood of 12 patients with diabetes mellitus and of 6 donors, were incubated in isotonic medium at 37 degrees within 180 min. Patterns of thiamin metabolism (activity of transketolase and TDP-effect), of glycolysis (content of glucose, lactate) and of energy metabolism (ATP, ADP, AMP and 2,3-DPG) were studied during the incubation. The ratios of energy-synthesizing and energy-consuming reactions were shifted towards the latter reactions in erythrocytes of the patients, which appears to be among the factors responsible for impairment of thiamin metabolism in diabetes. In order to correct the impairments found some drugs, such as insulin, thiamin, cocarboxylase, thiamin combined with adenine were added into the incubation medium. Insulin and cocarboxylase as well as insulin combined with thiamin and adenine exhibited the best effect on the patterns studied; these drugs normalized the vitamin B1 metabolism and improved the parameters of energy metabolism within 120 min of incubation in erythrocytes isolated from blood of patients with diabetes mellitus.
D000225:Adenine / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D003920:Diabetes Mellitus / Q000097:blood* / Q000188:drug therapy / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D013831:Thiamine / Q000097:blood* / Q000494:pharmacology; D013835:Thiamine Pyrophosphate / Q000494:pharmacology
D007328:Insulin; D000225:Adenine; C003434:thiamine bromide; D013835:Thiamine Pyrophosphate; D013831:Thiamine
Vzaimosviaz' narusheniĭ obmena tiamina i énergeticheskikh protsessov v éritrotsitakh bol'nykh sakharnym diabetom i puti ikh lekarstvennoĭ korrektsii.