Toxicity of organic acids for repair-deficient strains of Escherichia coli.
The wild-type strain and four DNA repair-deficient strains (uvrA6, uvrB5, recA56, and polA1) of Escherichia coli K-12 were treated with acetic acid, lactic acid, and p-aminobenzoic acid at pH 3.5 during their stationary phase of growth. All three acids were highly toxic to the polymerase-deficient strain. The greater sensitivity of the strain carrying the polA1 gene than its isogenic pol+ derivatives suggested that damage caused by acidity requires polA+ gene products for repair.
D010129:4-Aminobenzoic Acid / Q000633:toxicity*; D000085:Acetates / Q000633:toxicity*; D062365:Aminobenzoates / Q000633:toxicity*; D004260:DNA Repair; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005615:Freezing; D005798:Genes, Bacterial / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000633:toxicity*; D019344:Lactic Acid
D000085:Acetates; D062365:Aminobenzoates; D007773:Lactates; D019344:Lactic Acid; D010129:4-Aminobenzoic Acid