Flow-cytometric analysis of mitochondria-associated fluorescence in young and old human fibroblasts.
Rhodamine 123 fluorescence in populations of young and old human fibroblasts was analysed and quantified with a fluorescence-activated cell sorter (FACS). Old fibroblasts exhibited a higher mean relative fluorescence than young fibroblasts. Moreover, two distinct subpopulations were evident in the fluorescence distributions of old cells--but not in those of young cells.
D000367:Age Factors; D002469:Cell Separation; D005347:Fibroblasts / Q000032:analysis*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008928:Mitochondria / Q000032:analysis*; D020112:Rhodamine 123; D012235:Rhodamines
D012235:Rhodamines; D020112:Rhodamine 123