Gene transfer into intact plant cells by electroinjection through cell walls and membranes.
Tobacco mosaic virus (TMV) RNA was introduced directly into mesophyll cells of Nicotiana tabacum var. Samsun using electric-field pulses (electroinjection). The injected gene was successfully expressed in the recipient cells as judged by the assay for the virus coat protein using immunofluorescence and by the virus infectivity assay of the homogenate of the electroinjected cells for local lesions on tobacco leaves. As much as 50% of the cells that survived 24 days after electroinjection showed immunofluorescent specks.
D002213:Capsid / Q000032:analysis / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D005818:Genetic Engineering / Q000379:methods; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D012367:RNA, Viral / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics; D014027:Tobacco Mosaic Virus / Q000235:genetics*
D012367:RNA, Viral