Modulation of porcine peripheral blood-derived macrophage functions by in vitro infection with African swine fever virus (ASFV) isolates of different virulence.
Macrophages play an important role in defense against invading pathogens and neoplastic diseases. Their ability to maintain their functional characteristics is also important in the survival of the host. Loss of macrophage function during infection may be important in determining resistance or susceptibility of a host species. In this communication, we describe the effect of ASFV replication, in macrophages in vitro, on the functional characteristics of these cells. Infection with ASFV did not alter the expression of Fc receptors nor their ability to mediate ADCC. However, African swine fever virus was shown to modulate antibody mediated phagocytosis, chemiluminescence and chemotaxis. Similar modulation of certain macrophage functions was observed with ASFV isolates that differed in their virulence. We were therefore, unable to correlate the virulence of ASFV with the ability to alter macrophage function.
D000357:African Swine Fever / Q000276:immunology*; D000358:African Swine Fever Virus / Q000302:isolation & purification; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008264:Macrophages / Q000276:immunology*; D010587:Phagocytosis; D011863:Radioimmunoassay; D011961:Receptors, Fc / Q000032:analysis; D013552:Swine; D013997:Time Factors
D000954:Antigens, Surface; D000956:Antigens, Viral; D011961:Receptors, Fc