title: Demonstration of carboxyl and thiol protease activities in adult Schistosoma mansoni, Dirofilaria immitis, Angiostrongylus cantonensis and Ascaris suum.
abstract: Evidence has been presented showing two kinds of acidic protease activities in adult Schistosoma mansoni, Dirofilaria immitis, Angiostrongylus cantonensis and Ascaris suum. A haemoglobinolytic activity without adding any SH-containing compounds was maximal at pH 3.5, 2.5, 3.0 and 3.5 in S. mansoni, D. immitis, Angiostrongylus cantonensis and Ascaris suum respectively. The inhibitor studies demonstrated that this activity is ascribable to carboxyl protease(s). In the presence of dithiothreitol, activity on Azocoll (azo-dye coupled hide powder) was maximal at pH 4.6, 4.6, 3.5 and 5.6 in S. mansoni, D. immitis, Angiostrongylus cantonensis and Ascaris suum respectively. The effects of inhibitors demonstrated that this activity belongs to the thiol protease class. The intraspecific distribution of the protease activities was studied in some of the nematodes from which the organs could be anatomically separated. The distribution patterns of the haemoglobinolytic and azocollytic activities were quite different in An. cantonensis and much the same in As. suum. Based on the present results, acidic haemoglobinolytic activities reported in adult S. mansoni by other authors are thought to be due to carboxyl and thiol protease(s) respectively.
mesh_terms: D000801:Angiostrongylus / Q000201:enzymology; D000818:Animals; D001200:Ascaris / Q000201:enzymology; D016282:Aspartic Acid Endopeptidases; D001391:Azo Compounds / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D003546:Cysteine Endopeptidases; D004183:Dirofilaria immitis / Q000201:enzymology; D004229:Dithiothreitol; D004285:Dogs; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D009348:Nematoda / Q000201:enzymology*; D051381:Rats; D012550:Schistosoma mansoni / Q000201:enzymology*; D013552:Swine
chemical_list: D001391:Azo Compounds; D006454:Hemoglobins; C028470:Azocoll; D003094:Collagen; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D016282:Aspartic Acid Endopeptidases; D004229:Dithiothreitol