title: Studies of the combined use of steroid and Shosaikoto, one of the Kampohozai (Chinese traditional medicine), on pituitary adrenocortical axis function and immune responses.
abstract: The combined effects of Shosaikoto, one of the Kampohozai (Chinese traditional medicine), and prednisolone were examined for suppressive actions on pituitary adrenocortical axis function and immune response induced by prednisolone using rats and mice. The administration of Shosaikoto, 1.2 g/kg p.o., for 45 d showed a tendency to increase adrenal weight. By the combined use of Shosaikoto, 0.12 and 1.2 g/kg p.o., and prednisolone, 0.016 g/kg p.o., for 45 d, the decrease of adrenal weight induced by the treatment with prednisolone was restored. The administration of Shosaikoto, 1.2 g/kg p.o., elevated the blood corticosterone level. In the case of combined use, Shosaikoto, 0.12 and 1.2 g/kg p.o., inhibited the decrease of blood corticosterone level induced by the treatment with prednisolone, 0.004 g/kg p.o., and Shosaikoto, 1.2 g/kg p.o., inhibited the decrease of blood corticosterone level induced by the treatment with prednisolone, 0.016 g/kg p.o. On the other hand, the administration of Shosaikoto, 1.2 g/kg p.o., for 7 d reduced the number of hemolytic plaque forming cells (HPFC) in spleen cells. But, Shosaikoto, 1.2 g/kg p.o., administered for 7 d inhibited the decrease of the number of HPFC induced by the treatment with prednisolone, 0.01 and 0.03 g/kg s.c., for 3 d. Furthermore, Shosaikoto, 1.2 g/kg p.o., restored the number of rosette forming cell (RFC) which decreased by prednisolone, 0.03 g/kg s.c. The decrease of 7S HA titer of the serum by the treatment with prednisolone, 0.01 g/kg s.c., was also inhibited by the combination with Shosaikoto, 1.2 g/kg p.o.
mesh_terms: D000311:Adrenal Glands / Q000032:analysis / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003345:Corticosterone / Q000097:blood; D004365:Drugs, Chinese Herbal; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives / Q000032:analysis; D019695:Glycyrrhizic Acid; D006388:Hemagglutinins / Q000032:analysis; D006462:Hemolytic Plaque Technique; D007109:Immunity / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D009929:Organ Size / Q000187:drug effects; D010913:Pituitary-Adrenal System / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D010946:Plants, Medicinal; D011239:Prednisolone / Q000008:administration & dosage / Q000097:blood / Q000276:immunology / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012397:Rosette Formation; D012502:Sapogenins / Q000032:analysis; D012503:Saponins; D013154:Spleen / Q000033:anatomy & histology / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D006388:Hemagglutinins; D010936:Plant Extracts; D012502:Sapogenins; D012503:Saponins; C461321:shosaikoto-go-keishika-shakuyakuto; D019695:Glycyrrhizic Acid; D009828:Oleanolic Acid; D011239:Prednisolone; D006034:Glycyrrhetinic Acid; C025759:saikosaponin D; D003345:Corticosterone