Mapping of the triazine binding site to a highly conserved region of the QB-protein.
A number of herbicide classes, including the s-triazines and ureas (atrazine, diuron) inhibit photosynthetic electron transport via a direct interaction with the QB-protein. This protein, also known as the 32-kDa protein or herbicide binding protein, is believed to bind the plastoquinone QB, which functions as the second stable electron acceptor at the reducing side of Photosystem II. The site of covalent attachment of the photoaffinity herbicide analog azido-[14C]atrazine to the QB-protein of spinach chloroplast thylakoid membranes has been determined. Two amino acid residues are labeled; one residue is methionine-214, the other lies between histidine-215 and arginine-225. Both residues are within a region of the amino acid sequence which is highly conserved between the QB-protein and the L and M reaction center proteins of Rhodopseudomonas capsulata and R. sphaeroides. This region includes the site of a mutation which results in diuron resistance in Chlamydomonas reinhardi (valine-219). However, this region is well removed from point mutations at phenylalanine-255 (which gives rise to atrazine resistance in C. reinhardi) and at serine-264, (which results in extreme atrazine resistance in C. reinhardi and naturally occurring weed biotypes). The patterns of labeling and mutation imply that the quinone and herbicide binding site is formed by at least two protein domains.
D000345:Affinity Labels; D000595:Amino Acid Sequence; D001665:Binding Sites; D002268:Carboxypeptidases; D002734:Chlorophyll / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes; D010446:Peptide Fragments / Q000378:metabolism; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D011402:Pronase; D014227:Triazines / Q000378:metabolism*; D014357:Trypsin
D000345:Affinity Labels; D045342:Light-Harvesting Protein Complexes; D010446:Peptide Fragments; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D014227:Triazines; D002734:Chlorophyll; C049569:2-azidoatrazine; D002268:Carboxypeptidases; C022633:serine carboxypeptidase; D014357:Trypsin; D011402:Pronase; D003488:Cyanogen Bromide