The purification of fully active recombinant transforming growth factor alpha produced in Escherichia coli.
Recombinant human transforming growth factor alpha (TGF alpha), which is active as assessed by competition with epidermal growth factor (EGF) for binding to the EGF receptor, has been produced in Escherichia coli and separated from misfolded and inactive forms of recombinant TGF alpha using reverse-phase high performance liquid chromatography. The purified recombinant TGF alpha was used to produce a monoclonal antibody that binds to active TGF alpha specifically. The antibody was coupled to Sepharose and used as an independent method for purifying active TGF alpha. The EGF receptor binding activity of antibody affinity purified TGF alpha is comparable to that of high performance liquid chromatography-purified active TGF alpha, and is 0.55 mg of EGF eq/mg of TGF alpha. The disulfide arrangement of the active TGF alpha was determined after digestion with thermolysin, and found to be analogous to the disulfide arrangement previously determined for EGF (Savage, C. R., Hash, J. H., and Cohen, S. (1973) J. Biol. Chem. 248, 7666-7672).
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000302:isolation & purification*; D013820:Thermolysin; D015291:Transforming Growth Factors
D000596:Amino Acids; D004220:Disulfides; D010455:Peptides; D011994:Recombinant Proteins; D015291:Transforming Growth Factors; D013820:Thermolysin