Detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin-avidin ELISA more sensitive than the fluorescent antibody technique.
The presence of respiratory syncytial virus (RSV) was investigated by immunofluorescent antibody (IF) technique and by a biotin/avidin (BA) ELISA in 156 samples of nasopharyngeal secretions (NPS) obtained from infants and small children with acute respiratory disease. Of 70 RSV-IF-positive NPS, 68 were positive by BA-ELISA. Of 86 RSV-IF-negative NPS, eight were positive by BA-ELISA. BA-ELISA could detect 0.5-1.0 ng RSV-protein.
D000956:Antigens, Viral / Q000032:analysis / Q000302:isolation & purification; D001360:Avidin; D001710:Biotin; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000378:metabolism / Q000382:microbiology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000302:isolation & purification*; D010253:Respirovirus Infections / Q000175:diagnosis* / Q000276:immunology
D000956:Antigens, Viral; D001360:Avidin; D001710:Biotin