The use of alkaline phosphatase-conjugated anti-immunoglobulin with immunoblots for determining the specificity of monoclonal antibodies to protein mixtures.
D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis*; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007202:Indicators and Reagents; D008832:Microchemistry; D011506:Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002021:Buffers; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011506:Proteins; D000469:Alkaline Phosphatase