A comparison of the STAPH-Ident and STAPH-Trac systems to conventional methods in the identification of staphylococci isolated from bovine udders.
The STAPH-Ident and STAPH-Trac systems (Analytab Products, Plainview, N.Y.) were compared to conventional methods for identification of staphylococci isolated from bovine udders. The STAPH-Ident system identified 80.5% of isolates correctly. An additional 7.6% of Staphylococcus hyicus strains were delineated from S. epidermidis by characterizing acetoin and pigment production. Final accuracy of the STAPH-Ident system was 88.1%. The STAPH-Trac system identified 66.1% of isolates. Negative phosphatase tests for 42.3% of S. hyicus strains resulted in misidentification as S. simulans. Consequently, only 45.5% of S. hyicus isolates were identified correctly by the STAPH-Trac system. Minor modification of each system would permit accurate, rapid identification of staphylococci isolated from bovine udders.
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D005260:Female; D008321:Mammary Glands, Animal / Q000382:microbiology*; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D013210:Staphylococcus / Q000145:classification* / Q000302:isolation & purification
D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips