Selective proteolysis defines two DNA binding domains in yeast transcription factor tau.
Transcription of eukaryotic transfer RNA genes involves, as a primary event, the stable binding of a protein factor to the intragenic promoter. The internal control region is composed of two non-contiguous conserved sequence elements, the A and B blocks. These are variably spaced depending on the genes. tau, a large transcription factor purified from yeast cells, interacts with these two control elements as shown by DNase I footprinting, exonuclease digestion, dimethyl sulphate protection experiments and by analysis of point mutations. Here we used a limited proteolysis treatment to obtain a smaller form of tau with drastically altered DNA binding properties. A protease-resistant domain interacts solely with the B block region of tRNA genes.
D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D010447:Peptide Hydrolases / Q000494:pharmacology*; D012343:RNA, Transfer / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D004247:DNA; D012343:RNA, Transfer; D010447:Peptide Hydrolases