title: Effects of glucose and fructose added to the intraocular irrigating solutions on the rabbit ERG in vitro.
abstract: The addition of various concentrations of glucose to intraocular irrigating solutions for the retina was studied electrophysiologically using an in vitro eye-cup in the pigmented rabbit. Comparison of b-wave amplitudes demonstrated that, glucose at a concentration of 20 mM/l yielded the highest values. With this concentration, the b-wave amplitude was markedly enhanced to 250% at 90 minutes. With 5 mM/l glucose (normogycemic) solutions, the b-waves were decreased to below 20% at 60 minutes. A 40 mM/l glucose solution also increased the b-waves but to a lesser extent than 20 mM/l. These results might be due to the detrimental effects of osmolarity. However, under hypoxic conditions the amplitudes of b-waves obtained with 40 mM/l glucose solutions were greater than those with 20 mM/l glucose solutions. The addition of 10 mM/l fructose to the control solution had no remarkable effects suggesting that fructose is not a direct energy source for the in vitro retina.
mesh_terms: D000818:Animals; D004594:Electrophysiology; D004596:Electroretinography; D005632:Fructose / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D009883:Ophthalmic Solutions / Q000008:administration & dosage*; D009994:Osmolar Concentration; D011817:Rabbits; D012160:Retina / Q000502:physiology*; D007507:Therapeutic Irrigation; D013997:Time Factors
chemical_list: D009883:Ophthalmic Solutions; D005632:Fructose; D005947:Glucose