title: Grating film for use as a cover-slide in semi-quantitative and quantitative microscopic assessment of biological specimens.
abstract: We have patented a photographic film on which a series of parallel lines or concentric circles are reproduced, in various sizes, to provide a reference measurement for counting surfaces in a histological specimen without having to alter it in any way. The area of the preparation examined can thus be determined in numerical terms. This grating film can be used as a cover-slide for example, to calculate a surface that is different from normal, to map the distribution of different areas, etc. We use the grating film in studies of the condition of the endothelium of the carotid artery and aorta from rats fed a normolipidic diet.
mesh_terms: D000818:Animals; D006652:Histological Techniques; D008297:Male; D008853:Microscopy / Q000379:methods*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D010781:Photography; D051381:Rats; D011919:Rats, Inbred Strains