Isolation and characterization of a new peptide from hypothalamus and pituitary using a monoclonal antibody to LHRH.
A new peptide with LHRH-like immunoreactivity has been isolated from rat hypothalamus and pituitary using an immunosorbent prepared with monoclonal antibody to luteinizing hormone-releasing hormone (LHRH). This peptide contains part of the C-terminal sequence of LHRH and has a molecular weight of about 2000. It comprises over 20% of hypothalamic immunoreactivity recognized by the monoclonal antibody. Chromatographic and amino acid analysis data confirm the distinction between this peptide and LHRH. Immunocytochemical evidence is consistent with its being a normal constituent of the LHRH system, while its presence in the pituitary suggests that it may play a role in the regulation of this gland.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology*; D007031:Hypothalamus / Q000502:physiology*; D008297:Male; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000302:isolation & purification*; D010902:Pituitary Gland / Q000502:physiology*; D011863:Radioimmunoassay; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D007987:Gonadotropin-Releasing Hormone