[Detection of endotoxins in radiopharmaceutical preparations--II. Comparison of the sensitivity of methods using the rabbit and the Limulus amoebocyte lysate for the detection of endotoxins].
The rise of the rabbit internal temperature after i.v. injection of an endotoxin solution is proportional to concentration. Gelation of Limulus amoebocyte, when in presence of an endotoxin solution, is also related to concentration. We compared the sensitivity of these two methods. With our experimental procedure, the rabbit is sensitive to a 0.40 ng/mL solution and the Limulus amoebocyte lysate to a 0.14 ng/mL solution. The rabbit sensitivity increase is related to the per kilogramme injected volume, whereas sensitivity is not related to the volume to check in the case of the lysate.
D000818:Animals; D004340:Drug Contamination; D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli; D005334:Fever / Q000139:chemically induced*; D008033:Limulus Test; D011817:Rabbits; D011868:Radioisotopes / Q000009:adverse effects*; D012015:Reference Standards
D004731:Endotoxins; D011868:Radioisotopes
La recherche des endotoxines dans les préparations radiopharmaceutiques--II. Comparaison de la sensibilité des methodes utilisant le lapin et le lysat d'amoebocytes de Limule pour la détection d'endotoxines.