Is angiotensinogen a renin inhibitor and not the substrate for renin?
The cleavage of the angiotensinogen molecule by renin is slow. The rate constant for cleavage of the enzyme-substrate complex, Kcat (turnover number) is lower than usual for enzymes (0.6/s for the homologous human renin reactions and 0.15/s for the homologous mouse renin reaction). Thus each round of catalysis occurs in 1.7 s in the human and 6.7 s in the mouse reaction. The Kcat/Km values (10(4)-10(6) l/mol per s; Km, Michaelis constant) are high and in the range for protein inhibitors. The association constants (Ka) are (10(6)-10(7) l/mol) close to that of inhibitors. The slow rate seems to be favourable since homologous reactions are slower than heterologous. Angiotensinogen is structurally related to alpha1-antitrypsin. We conclude that kinetic as well as structural data indicate that angiotensinogen might be considered a protease inhibitor for renin.
D000808:Angiotensinogen / Q000378:metabolism*; D000809:Angiotensins / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D008970:Molecular Weight; D012083:Renin / Q000378:metabolism; D000092502:Renin Inhibitors
D000808:Angiotensinogen; D000809:Angiotensins; D012083:Renin; D000092502:Renin Inhibitors