Independent regulation of granulocyte-macrophage colony-stimulating factor and multi-lineage colony-stimulating factor production in T lymphocyte clones.
When murine T lymphocyte clones were cultured with purified recombinant IL 2, a dose-dependent increase in the production of granulocyte-macrophage colony-stimulating factor (GM-CSF) was observed. Whereas these clones produced both GM-CSF and multi-lineage CSF (multi-CSF) when cultured with concanavalin A, IL 2 induced the production of GM-CSF in the virtual absence of detectable multi-CSF. In addition, IL 2 synergistically enhanced the production of both GM-CSF and multi-CSF by some antigen- or Con-A-stimulated clones. Like Con-A-induced CSF production, GM-CSF production in the presence of IL 2 required protein synthesis but could occur in the absence of proliferation by the clone. Analysis of dose-response curves for stimulation of CSF production by Con A in the presence and absence of IL 2 suggested that Con A and IL 2 activated GM-CSF synthesis by different mechanisms. These results indicate that the coordinate production of two factors by a single T cell clone stimulated with Con A can be dissociated when the clone is stimulated with IL 2.
D000818:Animals; D002474:Cell-Free System; D002999:Clone Cells / Q000378:metabolism; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000032:analysis; D003513:Cycloheximide / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006098:Granulocytes; D006133:Growth Substances / Q000096:biosynthesis*; D016298:Hematopoietic Cell Growth Factors; D007376:Interleukin-2 / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D014176:Protein Biosynthesis; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D003470:Culture Media; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007376:Interleukin-2; D008070:Lipopolysaccharides; D003208:Concanavalin A; D003513:Cycloheximide