Amperometric enzyme-immunosensor based on ferrocene-mediated amplification.
Coimmobilized glucose oxidase and anti-T4 antiserum are employed in the construction of a biosensor for thyroxine measurements. The resulting enzyme-immunosensor yields an amplified response to T4 with a detection limit of 15 nM.
D015374:Biosensing Techniques; D004563:Electrochemistry; D005296:Ferrous Compounds; D005949:Glucose Oxidase; D007118:Immunoassay; D000075163:Metallocenes; D009942:Organometallic Compounds; D013974:Thyroxine / Q000032:analysis* / Q000276:immunology
D005296:Ferrous Compounds; D000075163:Metallocenes; D009942:Organometallic Compounds; D005949:Glucose Oxidase; D013974:Thyroxine; C004998:ferrocene