Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.
Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with various degrees of experience with immunofluorescence methodology.
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D009981:Influenza B virus / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral