Analysis of closely related genes by the use of synthetic oligonucleotide probes labeled to a high specific activity.
A method for labeling synthetic oligonucleotide probes to high specific activity is described. The method utilizes two partly complementary oligonucleotides that are labeled by a fill-in reaction using the Klenow fragment of DNA polymerase I and four alpha 32P-nucleoside triphosphates. Such probes can, in combination with Southern blot analysis, be used for routine analysis of individual genes in multigene families.
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods; D005796:Genes; D005803:Genes, Immunoglobulin; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes
D015345:Oligonucleotide Probes