Isolation and partial characterization of an eosinophil chemotactic factor from metacestodes of Taenia taeniaeformis (ECF-Tt).
Eosinophil chemotactic activity associated with protein extracts of Taenia taeniaeformis metacestodes was investigated. Chemotactic activity was associated with the nonbound protein after QAE cellulose chromatography of a 3 M KCl extract of homogenized larvae. When this material was precipitated with ammonium sulfate, activity was present in the 40 to 80% precipitate. Upon rechromatography on QAE cellulose equilibrated in a low ionic strength buffer, eosinophil chemotactic activity was retained by the gel and eluted after application of the NaCl gradient. Gel filtration of Sephacryl S-300 yielded an estimated m.w. of 91,000. Chromatofocusing revealed a broad peak of activity with a pI of 4.5 to 5.0. SDS-PAGE showed the active fraction migrated as a protein with a m.w. of 10,400. ECF-Tt had chemotactic and chemokinetic activity for equine eosinophils and murine eosinophils, but not for equine and murine neutrophils.
D000645:Ammonium Sulfate; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002415:Cats; D002630:Chemotactic Factors / Q000302:isolation & purification*; D002631:Chemotactic Factors, Eosinophil / Q000032:analysis / Q000302:isolation & purification* / Q000502:physiology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004804:Eosinophils / Q000502:physiology; D005590:Fractional Precipitation; D006736:Horses; D006358:Hot Temperature; D007525:Isoelectric Focusing; D007814:Larva / Q000032:analysis; D051379:Mice; D010447:Peptide Hydrolases; D051381:Rats; D013621:Taenia / Q000032:analysis*
D002241:Carbohydrates; D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil; D010447:Peptide Hydrolases; D000645:Ammonium Sulfate