Assembly of microfibrils in vivo and in vitro from (1----3)-beta-D-glucan synthesized by protoplasts of Saccharomyces cerevisiae.
Polymer chains of (1----3)-beta-D-glucan were dissolved with 1 M NaOH at 4 degrees C from native microfibrillar protoplast nets. The chains associated into microfibrils during NaOH neutralization or dialysis. In contrast to the native microfibrils which are of uniform width individually (10 to 20 nm) and arranged in flat bundles, the microfibrils formed in vitro showed no band formation and consisted of fibrous spindle-shaped subunits of variable width or loose elementary fibrils about 1.7 nm wide. X-ray diagrams of native nets indicated a fairly high crystallinity and were different for wet and dry specimens. They corresponded to those of paramylon. Precipitated glucans produced diagrams different from the former and revealing a lower crystallinity especially with the dry samples. The X-ray pattern, combined with other data, allowed the precipitated microfibrils to be identified as aggregates of molecular strands composed each of three intertwined helical glucan chains. Since these triple helical chains are about 1.7 nm wide the elementary fibrils of this width can represent only single triple-helical strands. These helices have 7 glucose residues per turn and therefore a low symmetry which explains the poor crystallizing properties. The 7 membered helix represents a basic difference with the well crystallized native glucan which is built of highly symmetrical triple helices with 6 glucose residues per turn. Since 6(1) helical conformation is not formed in vitro at normal temperatures its generation in vivo must be due to the action of synthesizing enzymes at the protoplast membrane. The intertwining of these helices and crystallization of the strands are determined by their symmetry and physical properties of the chains.(ABSTRACT TRUNCATED AT 250 WORDS)
D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D005936:Glucans / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008854:Microscopy, Electron; D011523:Protoplasts / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*
D005936:Glucans