Characterization of an enzyme immunoassay by means of specific interaction affinities.
Antigen--antibody interactions involved in an enzyme immunoassay are characterized by their specific affinities. The use of a nonlinear least-squares approach of the response versus dose dependence allows the extrapolation of experimental data, as well as qualitative and/or quantitative assumptions about the interactions studied. As a practical test, the present technique has important advantages over common titration methods, by avoiding the fixation of a cut-off limit for positive and negative samples and by determining the affinity of the specific interaction for any dose required.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D006041:Goats / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007700:Kinetics
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G