Quantitative homogeneous enzyme immunoassays for amitriptyline, nortriptyline, imipramine, and desipramine.
We describe specific EMIT homogeneous enzyme immunoassays for amitriptyline, nortriptyline, imipramine, and desipramine in patients' serum samples. Before analysis, an easily performed extraction step involving the use of 500 microL of sample and a 1-mL disposable column eliminates cross-reacting polar metabolites. The range of the standard curve for the first three drugs is 25 to 250 micrograms/L, and for desipramine is 50 to 500 micrograms/L. Within-run and between-run CVs are less than 10% throughout the range of the assays. Results for patients' samples obtained by this method and by "high-performance" liquid chromatography compare well, showing a slope range of 0.94-1.04 and correlation coefficients ranging from 0.93 to 0.96, depending on the assay.
D000639:Amitriptyline / Q000032:analysis*; D000929:Antidepressive Agents, Tricyclic / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D003891:Desipramine / Q000032:analysis*; D006801:Humans; D007099:Imipramine / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008722:Methods; D009661:Nortriptyline / Q000032:analysis*
D000929:Antidepressive Agents, Tricyclic; D000639:Amitriptyline; D009661:Nortriptyline; D007099:Imipramine; D003891:Desipramine