title: Novel heterophile chicken antigen: immunohistochemical localization using antisera to Mycobacterium smegmatis and possible association with lymphocyte maturation.
abstract: A novel heterophile antigen shared by Mycobacterium smegmatis and chicken tissues was demonstrated by the indirect immunoperoxidase method using antisera raised in rabbits immunized with a complete Freund's adjuvant containing killed Mycobacterium smegmatis as an immunostimulating component. This antigen was strongly expressed in medullary lymphocytes of the thymus and bursa of Fabricius, but was undetectable in lymphoid cells of the cortical regions of these organs. Only a few lymphocytes stained positively for the antigen in T- and B-cell areas of the spleen. These data suggest that the heterophile antigen is associated with the intrathymic and intrabursal maturation of chicken lymphocytes. The antigen was also detected in some nonlymphoid cells. It was not found in sheep erythrocytes, human and rat tissues or in killed bacillus Calmette--Guerin.
mesh_terms: D000818:Animals; D000910:Antibodies, Heterophile; D015478:Antigens, Heterophile / Q000032:analysis*; D002645:Chickens; D005620:Freund's Adjuvant / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D008213:Lymphocyte Activation; D009161:Mycobacterium / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D013950:Thymus Gland / Q000032:analysis / Q000166:cytology
chemical_list: D000910:Antibodies, Heterophile; D015478:Antigens, Heterophile; D007106:Immune Sera; D005620:Freund's Adjuvant