Isolation of pure LpB from human serum.
Low density lipoproteins (LDL), even after isolation from a narrow density cut and after several washes by preparative ultracentrifugation, are contaminated by 3-5% non-apoB proteins. Incubation of these LDL with artificial triglyceride-rich lipid emulsions (TGRP) removed all contaminating apoC and also, under certain conditions, apoA proteins. TGRP treatment did not, however, change the lipid composition and the flotation behavior of LDL. Residual apoE and albumin, amounting up to 0.5% of the apoB mass, were resistant to removal by TGRP treatment as well as by heparin-Sepharose column chromatography. ApoE and albumin could only be removed by immunoabsorption.
D001053:Apolipoproteins / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood; D004655:Emulsions; D006801:Humans; D007163:Immunosorbent Techniques; D008077:Lipoproteins, LDL / Q000097:blood*; D012709:Serum Albumin / Q000302:isolation & purification; D014280:Triglycerides; D014461:Ultracentrifugation
D001053:Apolipoproteins; D001055:Apolipoproteins B; D004655:Emulsions; D008077:Lipoproteins, LDL; D012709:Serum Albumin; D014280:Triglycerides