Immunological evidence for the involvement of cell wall proteins in phosphate uptake in the yeast Saccharomyces cerevisiae.
Immunological cross-reactivity between cell wall proteins obtained from two yeast genera (Candida tropicalis and Saccharomyces cerevisiae) is reported. Specific retention of two cell wall proteins from Saccharomyces cerevisiae by an immunoabsorbent column coupled with antibodies against phosphate binding protein 2(PiBP2) from Candida tropicalis allowed to generate antibodies against the proteins from S. cerevisiae. These antibodies were effective in inhibiting phosphate uptake by S. cerevisiae cells. The proteins from S. cerevisiae displayed a phosphate binding activity which was inhibited in the presence of the forementioned antibodies. These results and the observation that the amount of these proteins in the shock fluid was dependent of the growth conditions (i.e., in the presence or in the absence of phosphate) support the idea that these proteins are involved in the high affinity phosphate transport system.
D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology; D001692:Biological Transport; D002175:Candida / Q000276:immunology / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002473:Cell Wall; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D009997:Osmotic Pressure; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000276:immunology / Q000378:metabolism*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D002352:Carrier Proteins; D005656:Fungal Proteins; D028044:Phosphate-Binding Proteins; D010710:Phosphates