The preliminary characterization of mitogens secreted by embryonic chick wing bud tissues in vitro.
Embryonic chick wing bud tissues secrete diffusible mitogens when cultured in vitro (Bell & McLachlan, 1985). These molecules may play an important role in limb development since media conditioned by morphogenetically active regions of the wing bud possess greater mitogenic activity than media conditioned by non-morphogenetic regions. These studies show that while the chick-derived growth factors were mitogenic for mouse-derived NIH 3T3, 10T1/2 and NR6 cells and chick limb bud cells, they did not stimulate DNA synthesis in 3B11, PC13 END, normal rat kidney or bovine endothelial cells. Furthermore, the effects of chick-derived mitogens were synergistically enhanced by insulin and PGF 2 alpha but remained unaffected by ECDGF, EGF, FGF and MSA. These findings indicate that embryonic chick limb bud cells synthesize and secrete growth factors which resemble in function other well-characterized growth factors and in particular PDGF.
D000818:Animals; D002460:Cell Line / Q000187:drug effects; D002642:Chick Embryo; D015237:Dinoprost; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D008934:Mitogens / Q000378:metabolism; D010455:Peptides; D011460:Prostaglandins F / Q000494:pharmacology; D014921:Wings, Animal / Q000196:embryology* / Q000378:metabolism
D006133:Growth Substances; D007328:Insulin; D008934:Mitogens; D010455:Peptides; D011460:Prostaglandins F; C044343:embryonal carcinoma-derived growth factor; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D007335:Insulin-Like Growth Factor II; D015237:Dinoprost