In vitro growth potential of fibroblasts isolated from pigs with radiation-induced fibrosis.
Degenerative processes were studied in pig muscles irradiated with single doses of 30 or 40 Gy. Damaged muscle was gradually replaced by an invasive fibrotic tissue. As a control, surgical muscle exeresis was performed of the same size as the radiation-induced lesions at the same anatomical site. Primary cultures were set up comprising cells freshly extracted from normal dermis, or from tissue exhibiting either normal wound fibrosis or radiation-induced fibrosis. The growth potential of cells taken from the latter region far exceeded that of the two other types; attachment efficiency was higher, and fibronectin was detected early by immunofluorescence. These in vivo and in vitro observations imply that a pathological repair process occurs after localized irradiation.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D005356:Fibromyalgia / Q000473:pathology*; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D008297:Male; D009132:Muscles / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D013552:Swine
D005353:Fibronectins