Comparative immuno-removal: a novel method for estimating the concentration of an unknown immunoreactive compound.
We describe a method for estimating the molar concentration of an unknown immunoreactive compound. The amount of antibody required to bind half of a standard is compared to the amount required to bind half of an equal number of immunoreactive equivalents of the unknown. We demonstrate the utility of the method using a morphine antibody affinity resin and compounds structurally related to morphine.
D000818:Animals; D002417:Cattle; D003061:Codeine / Q000276:immunology; D003429:Cross Reactions; D007163:Immunosorbent Techniques; D007700:Kinetics; D009020:Morphine / Q000276:immunology*; D011863:Radioimmunoassay; D013797:Thebaine / Q000276:immunology
D013797:Thebaine; D009020:Morphine; D003061:Codeine