Ames Salmonella/mammalian-microsome testing of peptides and peptide synthesis reagents.
The Ames Salmonella/mammalian-microsome assay was used to evaluate the bacterial mutagenicity of 6 bioactive peptides and of 11 chemical reagents used in peptide synthesis. Samples of 2 reagents, bis(2-oxo-3-oxazolidinyl)phosphinic chloride and fluoren-9-ylmethyl chloroformate, showed mutagenic activity with strains TA100 and TA1535, and with TA1537, respectively. No mutagenic activity was found with the bioactive peptides or with the other 9 peptide synthesis reagents.
D000818:Animals; D002254:Carbonates / Q000009:adverse effects; D004151:Dipeptides / Q000009:adverse effects; D004305:Dose-Response Relationship, Drug; D005561:Formates / Q000009:adverse effects; D007202:Indicators and Reagents / Q000009:adverse effects*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens; D009842:Oligopeptides / Q000009:adverse effects; D010455:Peptides / Q000009:adverse effects* / Q000138:chemical synthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013388:Succinimides / Q000009:adverse effects; D013758:Tetragastrin / Q000009:adverse effects
D002254:Carbonates; D004151:Dipeptides; D005561:Formates; D007202:Indicators and Reagents; D009153:Mutagens; D009842:Oligopeptides; D010455:Peptides; D013388:Succinimides; D013758:Tetragastrin; C018241:carbobenzoxy chloride; C017330:N-formylmethionylphenylalanine; C029535:lysyl-tryptophyl-alpha-lysine; C001426:N-hydroxysuccinimide; C027600:bis(tert-butoxycarbonyl)oxide