An immunofluorescent method for a specific demonstration of granulocytes and some of their proteins (ECP and CCP).
Using p-benzoquinone as a fixative the non-specific fluorescence of granulocytes and especially the eosinophils is removed for both FITC and TRITC. In this way we have been able to detect the eosinophil cationic protein (ECP) and the chymotrypsin-like cationic protein (CCP) in human lung tissue and by double immunofluorescent labelling shown that these two proteins very likely are related to eosinophils respectively the neutrophils.
D000328:Adult; D016227:Benzoquinones; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D047091:Eosinophil Granule Proteins; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D011809:Quinones; D012260:Ribonucleases
D016227:Benzoquinones; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D011809:Quinones; C015078:chymotrypsin-like cationic protein, human; C004532:quinone; D012260:Ribonucleases