Demonstration of hapten augmentable plaques in the bromelain treated anti-mouse red blood cell system: putative evidence for anti-idiotypic regulation of autoantibody secretion.
Spleen cells from normal, nonautoimmune mice (C3H/HeN) spontaneously produce hemolytic plaques against autologous bromelain-treated red blood cells (BrMRBC). The number of anti-BrMRBC plaques detectable can be increased by including either a 3 M KCl extracted antigen from BrMRBCs or the hapten phosphorylcholine chloride (PC) as an antigenic analog in the plaque assay. Optimal PC concentration for augmenting the number of plaque forming cells (PFCs) was between 10(-7) and 10(-8) M. Incubation of spleen cells with an equal volume of 10(-7) M PC one, two, or three times resulted in the preparation of populations of cells which yielded increased numbers of PFCs. In addition, the number of anti-BrMRBC plaques of cells incubated three times could not be further increased by adding PC to the plaquing mixture. The eluate produced by the initial incubation of spleen cells with 10(-7) M PC specifically suppressed the anti-BrMRBC PFC response of these nonhapten augmentable cell populations (3 X eluted). These studies indicate that a naturally occurring autoantibody response is normally regulated by the presence of a molecule bound to the cell surface of autoantibody forming cells.
D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis*; D001789:Blood Group Antigens / Q000276:immunology* / Q000494:pharmacology; D001963:Bromelains / Q000276:immunology*; D002474:Cell-Free System; D004912:Erythrocytes / Q000187:drug effects; D006241:Haptens / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008214:Lymphocytes / Q000145:classification; D051379:Mice; D008809:Mice, Inbred C3H; D010767:Phosphorylcholine / Q000276:immunology; D013154:Spleen / Q000166:cytology
D001323:Autoantibodies; D001789:Blood Group Antigens; D006241:Haptens; D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine; D001963:Bromelains