[Amino acid and carbohydrate levels in Trichophyton verrucosum and homologous purified trichophytin].
The object of this study was to determine of aminoacids and sugar contents of T. verrucosum mycelium and of homologous purified trichophytin Tv-GP. The analysis of aminoacids contents was performed by ion-exchange chromatography on Czechoslovak analyser (type AAA-881) in acid and alkaline hydrolizates. Sugar were identified on gas chromatograph (PYE-105) by Sawerdeker and Sloneker technique. Seventeen identical aminoacids were determined in mycelium and trichophytin, but some quantity differences were observed. In mycelium there was 40.8% aminoacid nitrogen and 59.2% aminosaccharide nitrogen. In trichophytin, however, 93.5% nitrogen originated from aminoacids and only 6.5% from aminosaccharides. In both analized materials hexoses dominated: mannose, glucose and galactose. Besides trace quantities of D-glycero-D-mannoheptose, L-glycero-D-mannoheptose and L-glycero-D-glucoheptose were identified.
D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D000946:Antigens, Fungal / Q000032:analysis*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D066298:In Vitro Techniques; D014248:Trichophytin / Q000032:analysis*; D014249:Trichophyton / Q000276:immunology / Q000378:metabolism*
D000596:Amino Acids; D000946:Antigens, Fungal; D002241:Carbohydrates; D014248:Trichophytin
Skład aminokwasów i cukrów Trichophyton verrucosum oraz homologicznej oczyszczonej trychofityny.