Permanent preservation of whole alizarin red S skeletons by clearing and embedding in polyester resins.
A one-step clearing and embedding procedure for alizarin red S stained skeletons is described. Embryos are fixed in formalin, skinned and eviscerated. After staining in a 10 mg/liter solution of alizarin red S in 5% aqueous KOH, specimens are dehydrated in a graded series of acetone-polyester monomer solutions. Finally, the specimens are embedded at room temperature in the polyester resin. A special reusable metallic mold is described for embedment of large fetuses. Specimens previously cleared in glycerol can be processed with this method.
D000880:Anthraquinones; D001842:Bone and Bones / Q000033:anatomy & histology*; D005333:Fetus / Q000033:anatomy & histology; D006652:Histological Techniques; D006801:Humans; D012117:Resins, Synthetic; D012863:Skeleton
D000880:Anthraquinones; D012117:Resins, Synthetic; C004468:Alizarin Red S