title: Is phospholipid a required cofactor for the activity of mammalian signal peptidase?
abstract: To determine whether phospholipid is required for the activity of mammalian signal peptidase, the enzyme was partially purified from porcine pancreas and then extensively freed of phospholipid by SP-Sephadex C-50 chromatography. The delipidated enzyme showed signal peptidase activity, with a low concentration of detergent. Phospholipid was found to release the enzyme from the inhibition due to excess detergent.
mesh_terms: D000818:Animals; D003902:Detergents / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D006358:Hot Temperature; D008565:Membrane Proteins; D010743:Phospholipids / Q000502:physiology*; D012697:Serine Endopeptidases; D013552:Swine
chemical_list: D003902:Detergents; D008565:Membrane Proteins; D010743:Phospholipids; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase