Palindromic units from E. coli as binding sites for a chromoid-associated protein.
Several hundred copies of a highly conserved extragenic palindromic sequence, 20-40 nucleotides long, exist along the chromosome of E. coli and S. typhimurium. These have been defined as palindromic units (PU) or repetitive extragenic palindromes (REP). No general function for PUs has been identified. In the present work, we provide data showing that a protein associated with a chromoid extract of E. coli protects PU DNA against exonuclease III digestion. This provides the first experimental evidence that PU constitutes binding sites for a chromoid-associated protein. This result supports the hypothesis that PUs could play a role in the structure of the bacterial chromoid.
D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III