title: Evaluation of determination of theophylline concentrations by Seralyzer.
abstract: Determination of theophylline concentrations by Seralyzer in 28 paired serum and plasma samples disclosed almost identical results. Comparison of determinations by Seralyzer and enzyme-multiplied immunoassay in 31 paired serum samples disclosed significant underestimation by Seralyzer when recalibrated whenever results of analysis of a 15-micrograms/mL control exceeded limits of +/- 3 micrograms/mL. Determinations by Seralyzer did not differ significantly from enzyme-multiplied immunoassay with narrower limits of 15 +/- 2 micrograms/mL for Seralyzer calibration for 18 paired samples. Seralyzer results are sufficiently reliable for clinical use if the instrument is recalibrated whenever analysis of the 15-micrograms/mL control falls outside limits of +/- 2 micrograms/mL.
mesh_terms: D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D008722:Methods; D008991:Monitoring, Physiologic / Q000295:instrumentation*; D013806:Theophylline / Q000097:blood*
chemical_list: D013806:Theophylline