Regulatory role of human bone marrow fibroblasts in proliferation by granulocyte and macrophage colony-forming cells.
Human bone marrow fibroblasts (BMF) regulate the proliferation of granulocyte and macrophage colony-forming cells (GM-CFC). The mechanism used by BMF to regulate the proliferation of GM-CFC was investigated. When target marrows contained few spontaneous colonies, BMF enhanced granulopoiesis, while BMF-conditioned medium (BMF-CM) failed to do so. When target marrows contained large numbers of spontaneous colonies and when colony-stimulating factor (CSF) was present in the cultures, BMF and BMF-CM inhibited granulopoiesis. Adherent cells were necessary for the stimulation of granulopoiesis by BMF, but not for the inhibition of granulopoiesis by BMF and BMF-CM. Interaction of BMF with GM-CFC in dishes for a short time inhibited colony formation by GM-CFC. From these data, it has been concluded that the BMF regulate granulopoiesis and maintain the homeostasis of granulopoiesis through cell-cell interaction and factors produced by the BMF.
D000328:Adult; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008264:Macrophages / Q000166:cytology*
D003115:Colony-Stimulating Factors; D003470:Culture Media; D003094:Collagen