Glycidyl methacrylate copolymers as suitable supports for immobilizing antigens and antibodies.
Glycidyl methacrylate copolymers modified with 1,6-diaminohexane were activated with glutaraldehyde. Such activated supports were directly bound to the chosen antisera. Prepared immunosorbents contained ca. 40 mg of bound protein and were able to purify 2-5 mg of antigen (alkaline proteinases Esperase and Maxatase) per 1 g of solid support. Chaotropic agent (2.5 M ammonium thiocyanate in phosphate or Tris buffer) enabled antigen to be released quickly, the properties of the immunosorbent remaining unchanged.
D000179:Acrylates; D000906:Antibodies / Q000302:isolation & purification*; D000941:Antigens / Q000302:isolation & purification*; D010450:Endopeptidases / Q000302:isolation & purification; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D008689:Methacrylates; D010447:Peptide Hydrolases / Q000302:isolation & purification; D012697:Serine Endopeptidases
D000179:Acrylates; D000906:Antibodies; D000941:Antigens; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D008689:Methacrylates; D010450:Endopeptidases; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C021553:mesentericopeptidase; C007870:glycidyl methacrylate