title: Schwann cell marker defined by a monoclonal antibody (224-58) with species cross-reactivity. I. Cellular localization.
abstract: We have demonstrated by indirect immunofluorescence the cellular localization of a monoclonal antibody (mAb 224-58), produced after immunization of a mouse with human central nervous system (CNS) myelin. Serologically, mAb 224-58 was found to be specific for 3'-sulfomonogalactosylglycolipids, namely 3'-sulfogalactosylceramide (SGC) and 3'-sulfogalactosyl 1-O-alkyl ether 2-O-acylglycerol (seminolipid). This mAb did not bind to SGC-containing tissues such as kidney, liver, spleen, or brain, nor to muscle. However mAb 224-58 did stain positively mouse, rat, and human peripheral nerve sections. In these latter sections, mAb 224-58 was bound to Schwann cell bodies and processes. The specificity of mAb 224-58 for Schwann cells was ascertained on teased rat sciatic nerves and rat Schwann cell cultures. Cells positive for mAb 224-58 were also positive for laminin, and negative for Thy 1-1 antigens both in teased fibers and Schwann cell cultures. In addition, in teased nerve preparations, mAb 224-58-positive cells were also galactosylceramide (GalC)- and SGC-positive. Isolated Schwann cells also expressed 224-58 antigen, even after prolonged time in culture. On testis sections, which contain both SGC and seminolipid, the SGC-positive cells, i.e., the spermatogonia, were always 224-58-negative. But the other germinal cells were 224-58-positive. This suggests that although 224-58 does not discriminate between SGC and seminolipid in serological tests, these lipids in their naturally occurring membrane acquire a spatial configuration that renders them distinguishable to their respective antibody.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D005699:Galactosylceramides / Q000276:immunology; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000276:immunology; D010525:Peripheral Nerves / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000032:analysis*; D012584:Sciatic Nerve / Q000166:cytology; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D005699:Galactosylceramides; D006017:Glycolipids; C009233:seminolipid