Detection of DNA damage in human cells and tissue using sequencing techniques.
Methods have been developed that permit both identification and location of sites of alterations is defined DNA sequences. These methods can be extended to human tissues using the alphoid segment, which comprises 1% of the human genome. This segment can be isolated in ample quantities from human cells and tissues. Once purified and end labeled, this defined segment can be used to detect sites of altered DNA moieties by combining Maxam-Gilbert sequencing protocols with appropriate enzymatic probes and chemical techniques. These studies can be performed in cultured cells or in tissues obtained by surgical excision or autopsy.
D001345:Autoradiography; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D045647:DNA Glycosylases; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008837:Micrococcus / Q000235:genetics; D009154:Mutation; D009699:N-Glycosyl Hydrolases; D014466:Ultraviolet Rays
D004247:DNA; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases