[Hybridoma cultures using porcine serum not containing immunoglobulin and dialysis fractions].
The whole swine serum was treated with ammonium sulphate to precipitate immunoglobulins. The remained IgG was removed with the use of protein A-sepharose. The hybridoma cells producing monoclonal antibodies to lambda phage (class IgG) were cultured in Dalbecco's modified Eagle medium with addition of a 5% whole swine serum or of a treated unwhole one (final concentration of the protein being 3 mg/ml). Upon these conditions, hybridoma cells had similar growth rate and population density (1-1.3 X 10(6) cells/ml). Maximal antibody concentration was almost similar (80-90 mcg/ml). Purity of a sample of monoclonal antibodies isolated by the method of chromatography with the use of protein A-sepharose from supernatant containing the unwhole serum was no less than 99%, whereas it was considerably lower (12-15%) in the case of the whole serum.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D003584:Cytological Techniques; D003956:Dialysis; D005260:Female; D006825:Hybridomas / Q000187:drug effects* / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology*; D007136:Immunoglobulins / Q000302:isolation & purification*; D008297:Male; D013552:Swine
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007136:Immunoglobulins
Kul'tivirovanie gibridom s ispol'zovaniem svinoĭ syvorotki, ne soderzhashcheĭ immunoglobulinovoĭ i dializnoĭ fraktsiĭ.