Detection of lactate dehydrogenase B4 in human hemolysate of patients deficient in lactate dehydrogenase B subunit activity using enzyme immunoassay.
We developed a sensitive enzyme immunoassay system specific for human lactate dehydrogenase (LDH)-B4 with antiacetylated LDH-B4 Fab'-horseradish peroxidase conjugate. The enzyme immunoassay system was not interfered with by up to 0.3 mg/tube of hemoglobin. Thus, we measured LDH-B4 concentrations in the hemolysate of seven heterozygous individuals deficient in LDH-B subunit activity and eight normal individuals. We could not find a significant difference between the LDH-B4 concentrations in heterozygous and those in normal individuals. These results demonstrate that heterozygous individuals deficient in LDH-B subunit activity produce enzymatically inactive B subunits.
D001219:Aspartate Aminotransferases / Q000235:genetics; D004912:Erythrocytes / Q000201:enzymology; D006580:Genetic Carrier Screening; D006461:Hemolysis; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000172:deficiency; D046911:Macromolecular Substances
D007527:Isoenzymes; D046911:Macromolecular Substances; D007770:L-Lactate Dehydrogenase; D006735:Horseradish Peroxidase; D001219:Aspartate Aminotransferases