Enumeration of antibody-secreting cells by immunoprinting: sequential readout of different antibody isotypes on individual cell monolayers employing the ELISA plaque assay.
A technique is described for obtaining 'prints' of the antibody output of individual antibody-secreting cells (ASC), by repeated exposure of an immobilized ASC monolayer to coverslips coated with antigen. Zones of bound antibody on the coverslips (each being the 'print' of an individual ASC) are subsequently visualized by ELISA technology.
D000921:Antibody-Producing Cells / Q000276:immunology*; D002452:Cell Count; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006462:Hemolytic Plaque Technique; D007132:Immunoglobulin Isotypes / Q000032:analysis*
D007132:Immunoglobulin Isotypes