title: Rapid coagglutination test for the direct detection of group A streptococci from throat swabs.
abstract: A one-minute antigen detection test was compared with a conventional culture method for detecting group A beta-hemolytic streptococci. The test detects coagglutination between protein A and streptococcal antigen extracted directly from throat swabs. Of the 307 specimens tested, 66 (21.5%) were positive for group A streptococci by culture and 16 specimens (5.2%) were positive for other beta-hemolytic streptococci. The direct test agreed with the culture in 274 of 307 specimens (accuracy 89.3%). The sensitivity of the test was 86.4% (57/66), the specificity 90% (217/241), the positive predictive value 70.4% and the negative predictive value 96%. If only throat cultures with more than 100 colonies of group A streptococci per plate were considered, the sensitivity of the direct test rose to 96%. If only a strong agglutination was considered positive, the specificity of the direct test rose to 98%. Further studies are needed to determine whether this test could be used alone or in addition to culture.
mesh_terms: D000372:Agglutination Tests; D000942:Antigens, Bacterial / Q000032:analysis*; D006801:Humans; D010612:Pharyngitis / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology*; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000942:Antigens, Bacterial