The occurrence and distribution of alpha-hydroxy-acid dehydrogenase in some members of the order Kinetoplastida.
LDH-X is the isoenzyme of lactate dehydrogenase found in mammalian spermatozoa, occurring in cytosolic and mitochondrial locations. Gossypol strongly inhibits it, and the spermicidal action of this compound is attributed to the disruption of a reducing shuttle. The flagellated protozoan, Trypanosoma cruzi, contains an enzyme activity similar to LDH-X, called alpha-hydroxy-acid dehydrogenase, which is here shown to possess cytosolic and glycosomal components. The glycosome is a microbody-like organelle containing the early glycolytic enzymes. We postulate that the inhibition of replication of T. cruzi by gossypol derives from interference with glycosomal reducing shuttles. T. lewisi resembles T. cruzi in this respect.
D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000818:Animals; D003421:Crithidia / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase; D045022:Lactate Dehydrogenases; D007891:Leishmania / Q000201:enzymology*; D013045:Species Specificity; D014345:Trypanosoma / Q000201:enzymology*; D014346:Trypanosoma brucei brucei / Q000201:enzymology; D014349:Trypanosoma cruzi / Q000201:enzymology; D014350:Trypanosoma lewisi / Q000201:enzymology
D000429:Alcohol Oxidoreductases; D045022:Lactate Dehydrogenases; D007770:L-Lactate Dehydrogenase; C071195:D-lactate dehydrogenase; C021379:D-2-hydroxyacid dehydrogenase