Gas chromatography-mass spectrometry analysis of tert.-butyldimethylsilyl derivatives of 2-acetylaminofluorene and metabolites in isolated rat hepatocytes.
A new technique for the conversion of 2-acetylaminofluorene and several ring-hydroxylated metabolites to mono- and di-tert.-butyldimethylsilyl derivatives was developed to permit their analysis by gas chromatography-mass spectrometry in order to quantify the metabolism of 2-acetylaminofluorene incubated in freshly isolated rat hepatocytes. This new gas chromatography-mass spectrometry method allowed the separation, identification and quantitation of seven known metabolites comprising five arylhydroxylated compounds, 2-aminofluorene and N-hydroxy-2-acetylaminofluorene.
D015073:2-Acetylaminofluorene / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000378:metabolism*; D017646:Organosilicon Compounds; D051381:Rats; D012825:Silicon / Q000032:analysis / Q000378:metabolism*
D002250:Carbon Radioisotopes; D017646:Organosilicon Compounds; C009660:t-butyldimethylsilyl compounds; D015073:2-Acetylaminofluorene; D012825:Silicon