title: Divinylsulphone-activated agarose. Formation of stable and non-leaking affinity matrices by immobilization of immunoglobulins and other proteins.
abstract: Divinylsulphone-activated agarose is an attractive alternative to several of the activated supports usually used. Unlike CNBr-activated gels, it does not leak the immobilized protein at high pH. It reacts readily with proteins at near-neutral pH (unlike the epoxy-activated supports). Generally, divinylsulphone-activated agarose reacts with amino, hydroxyl, and sulphydryl groups, thus allowing immobilization of a wide spectrum of ligands. Moreover, it is available in an aqueous suspension free of organic solvents and neither requires time-consuming swelling nor washing.
mesh_terms: D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity; D004355:Drug Stability; D006041:Goats / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology; D012685:Sepharose / Q000032:analysis*; D013450:Sulfones
chemical_list: D007136:Immunoglobulins; D011506:Proteins; D013450:Sulfones; C009873:divinyl sulfone; D012685:Sepharose