Immunohistochemical characterization of inflammatory infiltrates at the site of bile duct injury in primary biliary cirrhosis.
Surgically obtained liver specimens from four patients with primary biliary cirrhosis (PBC), Stages I-II, were studied immunohistochemically using a broad panel of monoclonal antibodies. At the site of bile-duct injury (CNSDC), the inflammatory cells were recognized to be Coulter T-11+ (directed against all T-cells) and OKT-8+ (directed against cytotoxic/suppressor: C/S T-cells) cells. The MHC-class I antigen (i.e. HLA-A, B, C) was expressed weakly in the cytoplasm of the minority of damaged bile-duct epithelial cells, and the MHC-class II antigen (i.e. HLA-DR) was not expressed. Thus, OKT-8+ cells may play an important role in the immunologically mediated destruction of ductular epithelium in PBC. Strong MHC-antigen expression and OKT-8+ cell infiltration in destructive bile-duct lesions were not simultaneously observed. In the portal lymphocyte-rich areas, OKT-4+ (directed against helper/inducer: H/I T-cells) predominated over OKT-8+ cells. B-lymphocytes were present predominantly in the peripheral zones of the lymphoid aggregates. The distribution of helper/inducer T-cells and B-lymphocytes indicates that they may play a role in the induction of immunoglobulin in the lymphocyte-rich areas. The inflammatory mononuclear cells within the granuloma observed in one patient were Coulter T-11+, OKT-8+ and Leu-3a+3b+ (directed against helper/inducer T-cells) cells.
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D001652:Bile Ducts / Q000473:pathology; D005260:Female; D006099:Granuloma / Q000276:immunology / Q000473:pathology; D006680:HLA Antigens / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007249:Inflammation; D008105:Liver Cirrhosis, Biliary / Q000276:immunology* / Q000473:pathology; D008875:Middle Aged; D009000:Monocytes / Q000032:analysis / Q000276:immunology; D013601:T-Lymphocytes / Q000032:analysis / Q000145:classification
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II