Localization properties of fluorescence cytochemical enzyme procedures.
Fluorescence enzyme cytochemical procedures will contribute significantly to biomedical problems where knowledge of the enzymic composition of individual cells is important. Compared with the number of absorbance enzyme cytochemical methods, relatively few fluorescence procedures have been reported. In this paper, the merits of the described methods are discussed. A distinction is made between methods with and without a capture reaction. Only a few methods satisfy the requirement of accurate localization of the final product and high signal to noise ratios. Thus, there still is a need for valid fluorescence cytochemical enzyme methods. It is concluded that the bottle neck for valid fluorescence cytochemical enzyme methods is the development of efficient fluorogenic capture reactions for the primary enzyme products.
D000818:Animals; D002451:Cell Compartmentation; D004058:Diffusion; D004798:Enzymes / Q000032:analysis*; D005456:Fluorescent Dyes; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D010088:Oxidoreductases / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis; D010544:Peroxidases / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods
D004798:Enzymes; D005456:Fluorescent Dyes; D010088:Oxidoreductases; D010544:Peroxidases; D010447:Peptide Hydrolases