Immunohistochemical detection of glycogen phosphorylase isoenzymes in rat and human tissues.
Glycogen phosphorylase has at least three isoenzymes, i.e. muscle-, liver-, and brain-types. Antibodies have been raised against highly purified isoenzymes from rat muscle, liver and brain and found to react specifically to extracts from human muscle, liver and brain, respectively. Using these antibodies and the unlabelled antibody-enzyme method, each of the three isoenzymes has been localized in both rat and human tissues.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D006005:Phosphorylases / Q000032:analysis* / Q000145:classification; D051381:Rats; D013045:Species Specificity
D007106:Immune Sera; D007527:Isoenzymes; D006005:Phosphorylases