Purification and properties of three intracellular proteinases from Candida albicans.
Three intracellular proteinases termed A, B and C were purified to homogeneity from the unicellular form of the yeast Candida albicans. Enzyme A is an aspartic proteinase that acts on a variety of proteins. Its optimal pH is around 5 and it is displaced to 6.5 by KSCN. It is not significantly inhibited by PMSF, TLCK (Tos-Lys-CHCl2) or soybean trypsin inhibitor but it is inhibited by pepstatin. Its molecular weight is 60 000. Enzyme B is a dipeptidase that acts on esters or on dipeptides without blocks in either the carboxyl or amino ends. Its pH optimum is around 7.5 and the molecular weight is 57 000. It is inhibited by PMSF, TLCK and DANME (N2Ac-Nle-OMe). Proteinase C is an aminopeptidase with an optimum pH around 8. Its molecular weight was 67 000 when determined by SDS gel electrophoresis and 243 000 when determined by gel weight was 67 000 when determined by SDS gel electrophoresis and 243 000 when determined by gel filtration. It is active towards dipeptides in which at least one amino acid is apolar and is not active when the N-terminal amino acid is blocked. It is inhibited by EDTA or o-phenanthroline and activated by several divalent cations.
D016282:Aspartic Acid Endopeptidases; D002176:Candida albicans / Q000201:enzymology*; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D010450:Endopeptidases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D010436:Pepstatins / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; D013379:Substrate Specificity; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology; D029701:Saccharomyces cerevisiae Proteins
D016282:Aspartic Acid Endopeptidases; D002268:Carboxypeptidases; D010450:Endopeptidases; D007527:Isoenzymes; D010436:Pepstatins; D011190:Potassium Cyanide; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; D014107:Tosyllysine Chloromethyl Ketone; D014360:Trypsin Inhibitor, Kunitz Soybean; C014195:Streptomyces pepsin inhibitor; C022471:proteinase C; C023302:yeast proteinase B; D029701:Saccharomyces cerevisiae Proteins; C022325:aspartic proteinase A; C031375:pepstatin