Detection of group B streptococcal antigen in necropsy specimens using monoclonal antibody and immunoperoxidase staining.
A murine monoclonal antibody, which recognises various serotypes of group B Streptococcus in formalin fixed, paraffin embedded tissue, was used to show the organism in necropsy specimens of newborn infant lung by an indirect immunoperoxidase technique. The method seemed to be complementary to that of Gram staining, and may be successfully used to identify group B Streptococcus antigen in histopathological material.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn; D008168:Lung / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial