Purification of cytoplasmic precursors of yeast mitochondrial phenylalanyl-tRNA synthetase subunits.
Two polypeptidic precursors of yeast mitochondrial phenylalanyl-tRNA synthetase subunits were purified from the cytoplasm by immunoprecipitation with an insolubilized glutaraldehyde-treated IgG fraction, followed by two chromatographies on Sephadex G-200 and on DEAE-cellulose. Methionine was found as the N-terminal residue in both precursors, which exhibited N-terminal extensions.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D003593:Cytoplasm / Q000201:enzymology; D004792:Enzyme Precursors / Q000302:isolation & purification; D007158:Immunologic Techniques; D008928:Mitochondria / Q000201:enzymology; D008970:Molecular Weight; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D004792:Enzyme Precursors; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase