New candidates for GABAergic neurons in the rat cerebellum: an immunocytochemical study with anti-GABA antibody.
Cerebellar cortical neurons immunoreactive to anti-gamma-aminobutyric acid (GABA) antibody were examined in the rat. In addition to the Purkinje, Golgi, basket and stellate cells, spindle-shaped cells lying just below the Purkinje cell layer were found to be strongly immunoreactive to the antibody. By the combination of immunofluorescence and hematoxylin stainings, these GABA-positive cells were shown to be the Lugaro cells. Unlike the immunopositive small Golgi cell, the pale cell was not immunoreactive to the antibody.
D000818:Animals; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid