title: Insulin-like material in parotid and submaxillary salivary glands of normal and diabetic adult male mice.
abstract: Acid-ethanol extracts of homogenates from the parotid and submaxillary salivary glands of normal and streptozocin-induced diabetic adult male mice were investigated for insulin-like material. Extracts of both the parotid and submaxillary glands contained insulin-like immunoreactivity. The values were 156 +/- 72 ng/g wet tissue in the parotid and 104 +/- 36 ng/g wet tissue in the submaxillary gland. Fractionation of this material on Sephadex G-50 (superfine) columns revealed a single peak corresponding to the elution volume of isotopically labeled insulin. Isolated fat cells were stimulated by these extracts to convert [14C]glucose to 14CO2. This effect was blocked by preincubation with anti-insulin serum. It was observed with the avidin-biotin immunocytochemical technique that both the parotid and submaxillary glands of adult male mice possess a population of cells containing an insulin-like material. After intraperitoneal injection of streptozocin there was a marked decrease of insulin-like material extractable from both the parotid and submaxillary glands. However, this beta-cell cytotoxic agent did not completely destroy the salivary cells containing the insulin-like material. These data suggest that both the parotid and submaxillary salivary glands may be extrapancreatic sources of insulin in mice.
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006651:Histocytochemistry; D066298:In Vitro Techniques; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000032:analysis; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects; D010306:Parotid Gland / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D013363:Submandibular Gland / Q000032:analysis*; D014020:Tissue Extracts / Q000494:pharmacology
chemical_list: D007328:Insulin; D014020:Tissue Extracts; D005947:Glucose