Colour development of immunogold-labelled antibodies for light microscopy.
We describe an improvement of the immunogold technique, which is based on the colour development of silver-intensified immunogold signals. This method (referred to as the coloured-SIG technique) was found to be as sensitive as the silver-intensified immunogold method and more sensitive (in two of the three tested systems) than immunoenzymatic procedures, such as the peroxidase/antiperoxidase technique or the avidin-biotin system. The coloured SIG-method results in either a magenta-red or a cyan-blue final reaction product. Therefore, this new improvement of the immunogold technique should be useful in double-staining methods, immunoblot methods and conventional immunostaining methods.
D000906:Antibodies; D000954:Antigens, Surface / Q000032:analysis*; D001172:Arthritis, Rheumatoid / Q000473:pathology*; D006046:Gold; D006801:Humans; D007124:Immunoenzyme Techniques; D008853:Microscopy / Q000379:methods; D010003:Osteoarthritis / Q000473:pathology*; D014066:Palatine Tonsil / Q000473:pathology*; D013583:Synovial Membrane / Q000473:pathology*; D013601:T-Lymphocytes / Q000166:cytology*
D000906:Antibodies; D000954:Antigens, Surface; D006046:Gold