[Generation of HTLV-III-specific polypeptides in E. coli cells].
Cloning and expression in E. coli cells of a fragment of the env gene of HTLV-III virus is described. This fragment coding for from 294 to 757 aminoacid residue of virus protein was cloned in plasmid pUC 18. Conditions are described contributing to the regulated functioning of Lac-promoter allowing the expression of proteins toxic for E. coli. Solid-phase enzyme-immunoassay demonstrated a specific reaction of polypeptides synthesized in E. coli with an AIDS patient's serum. The sizes of these polypeptides were determined by the Western-blot method. They were found to be 18, 24, and 32 kilodaltons. The polypeptides synthesized in E. coli may apparently be used for preparation of test-systems for AIDS diagnosis.
D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005815:Genetic Code; D005822:Genetic Vectors; D006678:HIV / Q000235:genetics*; D007763:Lac Operon; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis / Q000235:genetics; D010957:Plasmids
D010455:Peptides
Poluchenie HTLV-III-spetsificheskikh polipeptidov v kletkakh E. coli.