An H-2 alloantiserum preserves beta-cell function in mice made diabetic by low-dose streptozocin.
The pancreatic beta-cell mass and function in C57BL/KsJ mice is markedly reduced the day after the last injection of five daily injections of a subdiabetogenic, 40 mg/kg, dose of streptozocin (STZ). In this study, we prepared an H-2 alloantiserum by injecting C57BL/6J mice (H-2b) with spleen lymphocytes from C57BL/KsJ (H-2d) mice. The alloantiserum given on five consecutive days, 5 h before each injection of STZ, did not prevent the initial beta-cytotoxic effect of STZ detected by perfusion of the pancreas and subsequent morphometric analysis of in situ dithizone-perfused pancreas. However, 12 days after the first injection of STZ, total insulin release in response to D-glucose, total pancreatic insulin, and pancreatic glucagon was greater in the alloantiserum-treated mice compared with controls receiving normal mouse serum. It is concluded that an H-2 alloantiserum may protect the function and amounts of beta-cells remaining after the initial five low-dose STZ injections.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology / Q000628:therapy*; D005934:Glucagon / Q000097:blood; D006183:H-2 Antigens / Q000276:immunology*; D007116:Immunization, Passive; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D001786:Blood Glucose; D006183:H-2 Antigens; D007328:Insulin; D005934:Glucagon