Assay of measles virus IgM and IgG class antibodies by use of peroxidase-labelled viral antigens.
Purified measles virions, nucleocapsid protein and crude lysate of measles infected cells were labelled with horseradish peroxidase, and used for the detection of IgM and IgG antibodies to measles virus by direct enzyme immunoassay. The assays consisted of three layers: anti-human IgM or IgG immunoglobulins on solid-phase, test serum specimen and enzyme labelled viral antigen. For the expression of the results, a standard curve was included in each test and the O.D. values were changed to arbitrary antibody units. Specificity and sensitivity of the assays were compared with indirect EIAs. The specificity studies included a collection of serum specimens containing either rheumatoid factor, antinuclear antibodies or IgM antibody specific for other viruses. The assays proved both reliable and simple to perform and sensitivity was slightly higher than that for indirect EIAs. However, specificity was dependent on the purity of the viral antigens. When crude infected cell lysate antigen was used, some nonspecific results were obtained, particularly with serum specimens containing antinuclear antibodies. When virion or nucleocapsid protein were used, no nonspecific reactions were obtained.
D000974:Antibodies, Antinuclear / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D008457:Measles / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D012217:Rheumatoid Factor / Q000032:analysis; D013696:Temperature; D013997:Time Factors
D000974:Antibodies, Antinuclear; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor