Intracellular site of insulin action: mitochondrial Krebs cycle.
Effect of insulin on the oxidation of carbons-1,4 and -2,3 of succinate and their incorporation into protein were studied in isolated rat hepatocytes and diaphragm muscle pieces. Oxidation of carbons-2,3 of succinate and their incorporation into hepatocyte protein were stimulated significantly by insulin. Insulin had only a trivial effect on 14CO2 formation from the carboxyl carbons of succinate. These data suggest that insulin affects only those carbons of succinate that are metabolized in the intramitochondrial Krebs cycle.
D000818:Animals; D002952:Citric Acid Cycle / Q000187:drug effects*; D006593:Hexokinase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008928:Mitochondria / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000187:drug effects; D008954:Models, Biological; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D007328:Insulin; D013386:Succinates; D019802:Succinic Acid; D006593:Hexokinase