1-Nitropyrene: a mutagenic product induced by the action of near ultraviolet light on 1-aminopyrene.
A solution of 1-aminopyrene in dimethyl sulfoxide exposed to an artificial source of near ultraviolet light (600 kJ/m2) induced significant direct-acting mutagenicity in the Ames/Salmonella plating assay utilizing strain TA98. High-performance liquid chromatography of this solution resulted in a fraction that was mutagenic on TA98 but inactive on a nitroreductase-deficient strain of Salmonella (TA98NR). This observation suggested the presence of a nitro-containing compound. Mass spectral analysis confirmed that 1-nitropyrene was the active photoproduct in this fraction. These data implicate photochemical transformation of primary aromatic amines as an alternative mechanism by which nitroaromatic compounds can be formed in the environment.
D002851:Chromatography, High Pressure Liquid; D004785:Environmental Pollutants; D013058:Mass Spectrometry; D009152:Mutagenicity Tests; D009153:Mutagens; D010084:Oxidation-Reduction; D011721:Pyrenes / Q000528:radiation effects*; D012486:Salmonella typhimurium / Q000187:drug effects; D014466:Ultraviolet Rays
D004785:Environmental Pollutants; D009153:Mutagens; D011721:Pyrenes; C004482:1-aminopyrene; C032668:1-nitropyrene