Composition of affinity-purified alpha-hemolysin of Escherichia coli.
Escherichia coli alpha-hemolysin was purified from culture supernatants by affinity chromatography, using a hemolysis-neutralizing monoclonal antibody ligand. Purified hemolysin contains several proteins and lipopolysaccharides. Thus, alpha-hemolysin exists as a macromolecular complex and may be exported from E. coli cells by outer membrane fragmentation.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000032:analysis*; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D008070:Lipopolysaccharides