A simple and rapid method for the determination of macrophage activating factor involving a new type of apparatus suitable for the measurement of macrophage chemiluminescence.
A simple and rapid method for the determination of macrophage activating factor is described. A new type of apparatus suitable for the measurement of macrophage chemiluminescence was devised, and the effect of lymphokines on macrophage activities was studied by measuring phorbol myristate acetate-induced luminol-dependent chemiluminescence. An outstanding feature of the new apparatus is that the plastic dish used for the cell culture can be used as the vessel for the chemiluminescence reaction. When thioglycollate-elicited ICR mouse peritoneal macrophages were incubated with lymphokines, their ability to generate chemiluminescence increased rapidly, reaching a maximal level at about 4 h, and then it progressively decreased to the control level at 8 h. Although this increasing effect of lymphokines on macrophage chemiluminescence was short-lived, it could be seen at a relatively low concentration, at which lymphokine-mediated cytotoxic activity of macrophages was not observed.
D000818:Animals; D001671:Biochemistry / Q000295:instrumentation*; D003601:Cytotoxicity Tests, Immunologic; D008163:Luminescent Measurements; D008165:Luminol; D008222:Lymphokines / Q000032:analysis* / Q000494:pharmacology; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013755:Tetradecanoylphorbol Acetate
D008222:Lymphokines; D016215:Macrophage-Activating Factors; D008165:Luminol; D013755:Tetradecanoylphorbol Acetate