Measurement of human and mouse anti-tetanus antibodies and isotype analysis by ELISA.
A rapid and sensitive enzyme immunoassay (ELISA) was developed for the quantitation of anti-tetanus antibodies. This technique was used to measure antibody levels in the plasma of immunized donors, in human anti-tetanus IgG preparations and in human and mouse hybridomas producing monoclonal antibodies to tetanus toxoid. The assay was capable of detecting antibody levels as low as 5 X 10(-4) IU/ml. By inclusion of an extra step involving antibodies to mouse Ig isotypes, a sandwich enzyme immunoassay (SEI) was developed which permitted determination of the Ig isotype of mouse anti-tetanus antibodies including tetanus-specific mouse monoclonal antibodies. SEI confirmed Protein A-Sepharose fractionation of mouse ascites fluid containing anti-tetanus antibody. The tetanus toxoid-coated plates have a shelf life of at least 1 year.
D000818:Animals; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D006801:Humans; D007124:Immunoenzyme Techniques; D007126:Immunoglobulin Allotypes / Q000032:analysis*; D051379:Mice; D013743:Tetanus Antitoxin / Q000032:analysis*; D013745:Tetanus Toxoid / Q000276:immunology
D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid