title: Determination of liothyronine and levothyroxine in thyroid preparations by liquid chromatography.
abstract: Liothyronine and levothyroxine were quantitatively determined in samples of commercial thyroid tablets and bulk powders. Samples were first hydrolyzed using a bacterial protease and then analyzed by high-performance liquid chromatography. Various hydrolysis conditions were investigated. The liothyronine and levothyroxine contents of commercial tablets and bulk powders were found to be approximately 8-11 micrograms and 25-43 micrograms, respectively, per 65 mg of thyroid. The stability of the iodothyronines in thyroid tablets was also investigated.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006868:Hydrolysis; D010447:Peptide Hydrolases; D011208:Powders; D013607:Tablets; D013974:Thyroxine / Q000032:analysis*; D014284:Triiodothyronine / Q000032:analysis*
chemical_list: D011208:Powders; D013607:Tablets; D014284:Triiodothyronine; D010447:Peptide Hydrolases; D013974:Thyroxine