Routine clinical determination of carotene, vitamin E, vitamin A, 25-hydroxy vitamin D3 and trans-vitamin K1 in human serum by straight phase HPLC.
A universal extraction procedure is described for fat-soluble vitamins in human serum. Methods are presented for routine quantitative analysis by isocratic straight phase HPLC with UV-detection of (alpha + beta)-carotene, vitamin E (alpha-tocopherol) and vitamin A (all-trans-retinol) in one single run, and of vitamin K1 (trans-phylloquinone) and 25-hydroxy vitamin D3 after sample clean-up using disposable reversed-phase cartridges. The limits of detection, precisions and selectivities of the developed assays are shown to be satisfactory after more than three years' experience. The routine clinical determination of fat-soluble vitamins can be performed in less than 5 mL of serum. Analyses of external quality control and randomly taken outpatient samples are shown to be of great value in assessing laboratory performance.
D002112:Calcifediol / Q000097:blood; D002338:Carotenoids / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood; D010837:Vitamin K 1 / Q000097:blood; D014815:Vitamins / Q000097:blood*
D014815:Vitamins; D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids; D010837:Vitamin K 1; D002112:Calcifediol