Identification of attenuating mutations on the reovirus type 3 S1 double-stranded RNA segment with a rapid sequencing technique.
Reovirus type 3 variants with mutations in the major neutralization domain of the sigma 1 protein have attenuated neurovirulence and restricted neurotropism. We devised a variation of the rapid RNA sequencing technique to facilitate the analysis of double-stranded RNA. We sequenced the S1 double-stranded RNA segment, which encodes the sigma 1 protein, of five attenuated reovirus type 3 variants. Four of the variants have changes in codon 419, and a fifth variant has a change at codon 340, all of which resulted in amino acid substitutions in the sigma 1 protein. We identified two sites on the reovirus type 3 sigma 1 protein that play a critical role in neurovirulence.
D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins; D003062:Codon / Q000235:genetics; D005809:Genes, Regulator; D005814:Genes, Viral; D005821:Genetic Techniques; D012089:Mammalian orthoreovirus 3 / Q000235:genetics* / Q000472:pathogenicity; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012087:Reoviridae / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D036022:Capsid Proteins; D003062:Codon; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; C031728:sigma 1 protein, reovirus