Immunohistochemical localization of apolipoprotein E in atherosclerotic lesions of the aorta and coronary arteries.
The distribution of apolipoprotein E (apo E) immunoreactive substances (IRS) in atherosclerotic lesions and lesion-free areas of the aorta and coronary arteries obtained from 17 autopsied cases was studied using a specific anti-apo E serum and the unlabeled peroxidase-antiperoxidase (PAP) method. In fatty streak lesions of the aorta, many cells containing apo E-IRS were found in the deeper layer of the intima and diffuse staining of apo E in the extracellular spaces was also noted. In more advanced lesions apo E-positive cells could not be found. Immunohistochemical findings of coronary arteries differed distinctly from those of the aorta in that the apo-E-positive cells were absent in the deeper layer of the intima. The endothelial cells of coronary arteries, but not those of the aorta, showed positive staining for apo E.
D000328:Adult; D000368:Aged; D001011:Aorta / Q000032:analysis*; D001057:Apolipoproteins E / Q000032:analysis* / Q000276:immunology; D001161:Arteriosclerosis / Q000378:metabolism*; D003331:Coronary Vessels / Q000032:analysis*; D004727:Endothelium / Q000502:physiology; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000032:analysis; D009131:Muscle, Smooth, Vascular / Q000032:analysis
D001057:Apolipoproteins E