Practical direct plaque assay for coliphages in 100-ml samples of drinking water.
A practical single-agar-layer plaque assay for the direct detection of coliphages in 100-ml samples of water was designed and evaluated. With this assay a 100-ml sample of water, an agar medium containing divalent cations, and the host Escherichia coli C (ATCC 13706) were mixed in a single container, and the mixture was plated on 10 14-cm-diameter petri dishes. It was more sensitive, reliable, and accurate than various other methods and proved rapid, simple, and economic.
D000362:Agar; D003090:Coliphages / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D004926:Escherichia coli; D010948:Viral Plaque Assay; D014871:Water Microbiology
D003470:Culture Media; D000362:Agar