Characterization of an alveolyn-like protein associated with mouse type 2 pneumocytes using immunochemical methods.
Delipidated proteins from a fraction of mouse lung homogenate enriched for lamellar bodies of type 2 pneumocytes were characterized by a combination of gel filtration and enzyme immunoassay for the presence of a surfactant-associated antigen. Immunoreactivity was associated with a protein of Mr 250-270,000 as well as its multimers and proteolytic fragments. The characteristics of the antigenic protein closely resembled those of a surfactant-associated protein termed alveolyn which is reported to be secreted by type 2 pneumocytes of various other species. Surfactant-associated proteins of M 32-38,000 in bronchoalveolar lavage fluid may be derived from this molecule by proteolysis.
D000818:Animals; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008168:Lung / Q000032:analysis / Q000166:cytology*; D051379:Mice; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011650:Pulmonary Alveoli / Q000032:analysis; D037662:Pulmonary Surfactant-Associated Protein A; D007507:Therapeutic Irrigation
D006023:Glycoproteins; D010446:Peptide Fragments; D037662:Pulmonary Surfactant-Associated Protein A; C445842:SFTPA1 protein, human