title: Cartilage grafting in facial reconstruction with special consideration of irradiated grafts.
abstract: The search for the perfect facial implant for reconstruction of the face continues. Cartilage, once thought to be an undesirable graft material because of its propensity for absorption, has regained popularity in the past decade. Various preparation techniques have been employed to ensure graft sterility and diminished absorption. An improved understanding of cartilage structure and physiology has shed considerable light on the host-graft relationship. Gamma irradiation is a time-honored method of preservation. An experiment was undertaken to investigate the physiology of irradiated cartilage grafts following prolonged implantation on the facial skeleton of sheep and dog. Merthiolate preserved grafts were used as controls. Direct observation, histochemical techniques, autoradiography, and transmission electron micrography were used to determine chondrocyte viability and matrix composition. It was surprising to note that following implantation of 16 to 72 months, complete resorption was seen in 87.7% of the irradiated grafts and in 43.8% of the Merthiolate stored controls. Many of the grafts acquired chondrocytes from the host and produced new proteoglycan matrix as well as undergoing some degree of ossification. A comparison to the clinical situation in humans is made.
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002356:Cartilage / Q000032:analysis / Q000166:cytology / Q000502:physiology / Q000528:radiation effects / Q000637:transplantation*; D004285:Dogs; D005147:Facial Bones / Q000601:surgery*; D005720:Gamma Rays; D006085:Graft Survival / Q000528:radiation effects*; D006801:Humans; D010012:Osteogenesis; D012756:Sheep; D013518:Surgery, Plastic / Q000379:methods; D013849:Thimerosal; D014021:Tissue Preservation; D014182:Transplantation, Autologous
chemical_list: D013849:Thimerosal