title: Specific detection of noradrenaline in the rat brain by using antibodies.
abstract: Noradrenaline (NA) was conjugated to different protein carriers with glutaraldehyde. During the catecholamine conjugate synthesis, precautions were taken in order to preserve the structure of NA. Anti-NA antibodies were raised in rabbits and tested using a modified enzyme-linked immunosorbent assay and immunocytochemistry. The most immunoreactive antigen was found to be the reduced NA conjugate. The octopamine conjugate was recognized by the NA antiserum 16 X less than the reduced NA conjugate. The other conjugates, dopamine, L-dihydroxyphenylalanine and adrenaline were poorly recognized by the anti-NA antibodies. A good correlation was found between results obtained using the in vitro enzyme-linked immunosorbent assay and immunocytochemistry.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001921:Brain / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D008125:Locus Coeruleus / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits / Q000276:immunology; D051381:Rats; D013378:Substantia Nigra / Q000378:metabolism
chemical_list: D009638:Norepinephrine