A method for screening penicillin G acylase-producing bacteria by means of 2-nitro-5-phenylacetaminobenzoic acid test paper.
A simple, rapid assay for screening penicillin G acylase-producing bacteria is presented. The method is based on the formation of yellow 2-nitro-5-aminobenzoic acid by penicillin G acylase acting on 2-nitro-5-phenylacetaminobenzoic acid (NIPAB). NIPAB test paper is briefly applied to bacterial colonies on the agar surface, which are subsequently scored individually on the paper by color; bright yellow indicates the presence of penicillin G acylase, natural color its absence. The present method is suitable not only for screening penicillin G acylase-production by a variety of bacteria but also for detection from a large number of transformant colonies of clones containing a gene encoding for the enzyme.
D000581:Amidohydrolases / Q000032:analysis*; D062365:Aminobenzoates; D001407:Bacillus / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology; D009579:Nitrobenzoates; D010209:Paper; D010399:Penicillin Amidase / Q000032:analysis* / Q000096:biosynthesis
D062365:Aminobenzoates; D003470:Culture Media; D009579:Nitrobenzoates; C008641:6-nitro-3-phenylacetamidobenzoic acid; D000581:Amidohydrolases; D010399:Penicillin Amidase