Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems.
A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The characteristic morphology of neurons is maintained, together with the diversity of active channels evident in the intact nervous system.
D000375:Aging; D000818:Animals; D001921:Brain / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D004558:Electric Stimulation; D006168:Guinea Pigs; D008564:Membrane Potentials; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure