Phagocytosis of light- and dark-adapted rod outer segments by cultured RPE cells: a reassessment.
Rod outer segments (ROS) isolated from adult rat retinas are phagocytized by cultured rat retinal pigment epithelial (RPE) cells. Using a double immunofluorescent labeling procedure, we have compared the binding and ingestion of ROS isolated at different times of the day. After 2 hr of incubation, approximately 98% of the ROS are ingested, while 2% are still attached to the RPE cell surface, irrespective of the time of day or lighting conditions under which the ROS are isolated. These findings differ from those reported earlier, using a radioactive method for quantitating ROS phagocytosis (Hall, 1978).
D000221:Adaptation, Ocular; D000818:Animals; D002478:Cells, Cultured; D002940:Circadian Rhythm; D005455:Fluorescent Antibody Technique; D008027:Light; D010587:Phagocytosis; D010786:Photoreceptor Cells / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000502:physiology*; D051381:Rats; D012374:Rod Cell Outer Segment / Q000502:physiology*