Formation of prostanoids in human umbilical vessels perfused in vitro.
Four major prostanoids (6-keto-PGF1 alpha, PGE2, PGF2 alpha and TXB2) were measured by specific radioimmunoassays in the outputs from human umbilical vessels perfused in vitro. As evaluated by scanning electron microscopy (SEM) only few blood platelets were attached to the vessel wall. After an initial flush with decreasing concentrations of all four prostanoids, a stable stage was reached, lasting for 4-5 hours. During this stage the production could be inhibited by indomethacin and only slightly stimulated with arachidonic acid. The TXA2 synthetase inhibitor UK 38485 depressed the TXB2 production, while only slightly affecting the other three prostanoids at very high concentrations. The arteries produced relatively more 6-keto-PGF1 alpha than did the vein.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D015237:Dinoprost; D015232:Dinoprostone; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D011863:Radioimmunoassay; D013929:Thromboxane B2 / Q000096:biosynthesis*; D014469:Umbilical Arteries / Q000378:metabolism*; D014471:Umbilical Veins / Q000378:metabolism*
D007093:Imidazoles; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; C039242:dazmegrel; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin