[Application of the vapor test for the detection and immunologic determination of Escherichia coli heat labile enterotoxin].
The principle of this thin-layer immunoassay (vapour condensation technique or TVAP) is based on the ability of antibodies to absorb firmly to polystyrene surfaces and to retain their reactivity. A condensation pattern consisting of large confluent water drops is noticable when an antibody-antigen reaction takes place. We used this technique to detect and assay the Escherichia coli heat-labile enterotoxin (ETEC LT+) and compared the results of 53 strains (40 positives and 13 negatives) with single radial immune haemolysis, Gm1-ELISA and Vero cell culture tests. With the reagents used, this reaction was specific for a toxin dilution up to 1/14. As little as 0.025 micrograms/ml of cholera toxin could be detected. The TVAP-test is simple, rapid and cost-effective. It is thus quite suitable for use in diarrhoeal endemic areas.
D000818:Animals; D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D002460:Cell Line; D002522:Chlorocebus aethiops; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000032:analysis; D029968:Escherichia coli Proteins; D006461:Hemolysis; D007118:Immunoassay / Q000379:methods; D007668:Kidney; D013045:Species Specificity
D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli
Application du test à la vapeur à la détection et au dosage immunologique de l'entérotoxine thermolabile de Escherichia coli.