title: Natural transmission of Dirofilaria immitis by Aedes aegypti.
abstract: The Liverpool strain of the mosquito Aedes aegypti was infected with microfilariae of the canine heartworm, Dirofilaria immitis, and was used to transmit heartworm larvae to three dogs. Methods of confirming heartworm infection in these dogs included the modified Knott's test, a commercial enzyme-linked-immunosorbent assay (ELISA), an indirect fluorescent antibody (IFA) test, and post-mortem examination.
mesh_terms: D000330:Aedes; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D004183:Dirofilaria immitis / Q000276:immunology / Q000302:isolation & purification; D004184:Dirofilariasis / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D004283:Dog Diseases / Q000469:parasitology / Q000635:transmission*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D007303:Insect Vectors; D008842:Microfilariae / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000909:Antibodies, Helminth