title: Ultrastructure of in vivo fertilization in the goat.
abstract: In vivo fertilization of goat eggs has been studied by electron microscopy. Eggs were recovered from superovulated or natural cyclic goats, 32 to 52 hours after the onset of oestrus; only eggs recovered between 46 and 52 hours were fertilized. Spermatozoa penetrated the zona pellucida tangentially leaving vesiculated products of the acrosome reaction at the zona surface. As sperm penetrated into the ooplasm, the second meiotic division completed and cortical granule exocytosis occurred. However a few unreacted cortical granules usually remained in the cortex of the fertilized eggs, adjacent to the plasma membrane. After swelling the two pronuclei presented similar ultrastructural morphology: they contained small, compact, agranular nucleoli and unevenly distributed chromatin. The cytoplasm in close vicinity to the apposed pronuclei contained large stacks of annulate lamellae, smooth endoplasmic reticulum, prominent Golgi complexes, as well as dense areas of unidentified material. The abundance of cytoplasmic organelles near the pronuclei might be the expression of intensive metabolic activity. Conversely, in the cortex of fertilized ova several large organelles-free cytoplasmic areas were randomly distributed.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure; D005260:Female; D005307:Fertilization in Vitro; D006041:Goats / Q000196:embryology*; D008297:Male; D008854:Microscopy, Electron; D009865:Oocytes / Q000648:ultrastructure*; D013084:Sperm-Ovum Interactions