title: Transplantation of cryopreserved hepatocytes or liver cytosol injection in the treatment of acute liver failure in rats.
abstract: Intraperitoneally injected cryopreserved hepatocytes or liver cytosol were used for the treatment of acute galactosamine-induced liver failure in rats. Both methods were similarly capable to improve the survival rates as compared to control groups. Furthermore, a new viability test for transplanted hepatocytes, using a nuclearmedical tracer technique, was evaluated in this study. This test demonstrated viability of intraperitoneally transplanted hepatocytes for at least 6 days after operation. Cytosol injection should be preferred to hepatocyte transplantation in the treatment of acute liver failure, avoiding the problems of cell storage and post-transplant immunosuppression.
mesh_terms: D000208:Acute Disease; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D002469:Cell Separation; D002470:Cell Survival; D003600:Cytosol; D005340:Fibrinogen / Q000032:analysis; D005615:Freezing; D005688:Galactosamine / Q000633:toxicity; D007274:Injections, Intraperitoneal; D008107:Liver Diseases / Q000628:therapy*; D016031:Liver Transplantation; D008297:Male; D010314:Partial Thromboplastin Time; D051381:Rats; D011917:Rats, Inbred Lew; D014021:Tissue Preservation
chemical_list: D005688:Galactosamine; D005340:Fibrinogen; D001219:Aspartate Aminotransferases; D001663:Bilirubin