Effect of a 2-methylthio-N6-isopentenyladenosine deficiency on peptidyl-tRNA release in Escherichia coli.
We examined the effect of miaA, a mutation conferring a deficiency in 2-methylthio-N6-isopentenyladenosine in tRNA, on patterns of peptidyl-tRNA accumulation in Escherichia coli strains deficient in peptidyl-tRNA hydrolase activity. A specific reduction in peptidyl-tRNA accumulation was seen for tRNAs which normally contain the 2-methylthio-N6-isopentenyladenosine modification. These results provide new evidence in support of the ribosome editor model, which links peptidyl-tRNA release to mistranslation events.
D000241:Adenosine / Q000031:analogs & derivatives*; D002474:Cell-Free System; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007541:Isopentenyladenosine / Q000031:analogs & derivatives* / Q000172:deficiency; D009154:Mutation; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism
D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-; C008503:2-methylthio-N-6-isopentenyladenosine; D007541:Isopentenyladenosine; D012343:RNA, Transfer; D000241:Adenosine