Cellular localization of the Ro/SS-A antigen.
The cellular localization of the Ro/SS-A antigen was defined using indirect immunofluorescence and eight monospecific anti- Ro/SS-A antisera which were identified by immunoblotting. Several mammalian tissues were used as substrates. The Ro/SS-A antigen was located mainly in the nucleus of dog liver and Hep-2-cells, and anti-Ro sera had produced a speckled staining pattern. Cytoplasmic fluorescence appeared only in one serum. IgG was the predominant immunoglobulin with anti-Ro/SS-A activity; five sera had complement fixing activity. The sera absorption studies with partially purified Ro/SS-A antigen and the negativation of the ANA tests had confirmed the specificity of our findings.
D000042:Absorption; D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis; D000941:Antigens / Q000276:immunology*; D001324:Autoantigens / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D002477:Cells / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000276:immunology; D051379:Mice; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014018:Tissue Distribution; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D000941:Antigens; D001324:Autoantigens; D007106:Immune Sera; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human