Chromium (VI) comutagenesis: characterization of the interaction of K2CrO4 with azide.
In a previous report chromate potentiated the mutagenicity of sodium azide, apparently by affecting repair and/or replication of DNA. Further evidence in support of such a mechanism for chromate potentiation is reported here. Chromate does not react directly with azide or its major mutagenic metabolite, azidoalanine, eliminating such reactions as possible mechanisms for potentiation. Further, azide was unable to potentiate the mutagenicity of chromate in Salmonella typhimurium strain TA104, which is sensitive to chromate mutagenicity but not to azide. Thus, it appears that the potentiation is not due to an action of azide in modulating chromate mutagenicity. Finally, the interaction was not altered by deficiency in recA gene product in S typhimurium GW19, nor by enhancement of SOS repair in the pKM101 containing strain TA100. Thus, induction of recA-dependent functions seems to play no role in the comutagenic actions of chromate. The simplest explanation for potentiation seems to be that chromate is able either to limit error-free recovery from azide-induced DNA damage or to promote error-prone repair or error-prone processing at sites of lesions.
D000409:Alanine / Q000031:analogs & derivatives / Q000633:toxicity; D001386:Azides / Q000008:administration & dosage* / Q000633:toxicity; D001711:Biotransformation; D002840:Chromates / Q000008:administration & dosage*; D004260:DNA Repair / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004357:Drug Synergism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage*; D017680:Potassium Compounds; D012486:Salmonella typhimurium / Q000187:drug effects
D001386:Azides; D002840:Chromates; D009153:Mutagens; D017680:Potassium Compounds; C027373:potassium chromate(VI); C039309:azidoalanine; D000409:Alanine