title: Stimulation of phosphatidylcholine synthesis by insulin and ATP in isolated rat adipocyte plasma membranes.
abstract: Added individually or together, insulin and/or ATP significantly and rapidly increased the concentration of phosphatidylcholine in an enriched plasma membrane preparation from rat adipocytes. The increase in phosphatidylcholine synthesis mediated by insulin or ATP was suppressed by the phospholipid methyltransferase inhibitor, S-adenosylhomocysteine. These results suggest that the activity of phospholipid methyltransferase from adipocyte plasma membranes may be increased by phosphorylation and that insulin may further increase the activity of the phosphorylated phospholipid methyltransferase by an alternative pathway.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000273:Adipose Tissue / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008780:Methyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D050918:Phosphatidylethanolamine N-Methyltransferase; D010714:Phosphatidylethanolamines / Q000378:metabolism; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000494:pharmacology
chemical_list: D007328:Insulin; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D000255:Adenosine Triphosphate; D012435:S-Adenosylhomocysteine; D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase