Comparative analysis of Haemophilus influenzae type b and Escherichia coli J5 lipopolysaccharides.
Haemophilus influenzae type b (HIB) and Escherichia coli J5 (J5) lipopolysaccharides (LPS) were examined to explore the basis of previously observed cross-protection. HIB-LPS and J5-LPS contained ketodeoxyoctonate, glucose, glucoheptose and glucosamine as common carbohydrate moieties, and laurate, myristate, beta-hydroxymyristate and palmitate as common fatty acids, although in different ratios. J5-LPS was five times more lethal than HIB-LPS for chick embryos. Weak serological cross-reactivity was observed by haemagglutination and two-dimensional immunoelectrophoresis. No significant cross-reactivity was demonstrated by enzyme-linked immunosorbent or toxicity-neutralisation assays. The cross-reactivity observed between HIB-LPS and J5-LPS was probably due to common components in the core glycolipid.
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001681:Biological Assay; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D003429:Cross Reactions; D004926:Escherichia coli / Q000032:analysis* / Q000276:immunology; D005227:Fatty Acids / Q000032:analysis; D006193:Haemophilus influenzae / Q000032:analysis* / Q000276:immunology; D006388:Hemagglutinins / Q000032:analysis; D007123:Immunoelectrophoresis, Two-Dimensional; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D009500:Neutralization Tests
D001426:Bacterial Proteins; D002241:Carbohydrates; D005227:Fatty Acids; D006388:Hemagglutinins; D008070:Lipopolysaccharides