High technology diagnostics: detection of enterotoxigenic Escherichia coli, using DNA probes.
A relatively new technique termed colony blot hybridization appears to be a reliable replacement for the ligated porcine gut loop assay previously used to detect stable toxin-B producing Escherichia coli. The highly reproducible blot assay enables screening of large numbers of isolants for the presence of E coli stable-B toxin genes, thus avoiding variations in phenotypic expression and poor repeatability inherent in the in vivo assays. Availability of this diagnostic test can aid practitioners in identifying enterotoxigenic E coli related disease in swine and in determining the benefits of vaccination programs.
D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D004247:DNA / Q000235:genetics; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000662:veterinary*; D066298:In Vitro Techniques; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D004768:Enterotoxins; D004247:DNA