Restoration of in vitro responsiveness of xid B cells to TNP-Ficoll by 8-mercaptoguanosine.
8-Mercaptoguanosine (8sGuo) has been reported to enhance responses of normal mice to the type 2 antigen trinitrophenol (TNP)-Ficoll. In this report, we demonstrate that this immune adjuvant restores the immune responsiveness of B cells from mice with the x-linked immune defect (xid), which are nonresponsive to the type 2 antigen TNP-Ficoll. The data demonstrate that TNP-Ficoll, which by itself cannot stimulate anti-TNP responses in CBA/N mice, is able to initiate the initial steps of cell activation in xid B cells and render them sensitive to the subsequent differentiative effects of 8sGuo. We propose that the unresponsiveness of xid B cells to type 2 antigens reflects not the inability of these antigens to stimulate xid B cells from G0 to G1, but rather the inability of these antigen-activated cells to respond to a second signal to which these immune defective B cells are poorly responsive and can be substituted for by 8sGuo.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009578:Nitrobenzenes / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013872:Thionucleosides / Q000494:pharmacology*; D014301:Trinitrobenzenes / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D009578:Nitrobenzenes; D011134:Polysaccharides; C020529:TNP-ficoll; D013872:Thionucleosides; D014301:Trinitrobenzenes; D006151:Guanosine; D005362:Ficoll; C037031:8-thioguanosine