Differential reactivity of 9-NH2-ellipticine on apurinic and apyrimidinic sites in circular DNA.
Endonucleases for apurinic sites as well as chemical compounds reacting with aldehydes do not generally differentiate between apurinic and apyrimidinic sites. We have studied the effect of the apurinic site reagent, 9-NH2-ellipticine, on apyrimidinic sites enzymatically generated on PBR322 DNA and compared it to its' action on apurinic PM2 and PBR322 DNAs. In conditions where this compound induces breakage of apurinic sites, it does not display any action on apyrimidinic sites.
D000470:Alkaloids / Q000494:pharmacology*; D002942:Circular Dichroism; D045647:DNA Glycosylases; D004269:DNA, Bacterial / Q000187:drug effects; D004270:DNA, Circular / Q000187:drug effects*; D004611:Ellipticines / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics; D009699:N-Glycosyl Hydrolases; D011687:Purines; D011743:Pyrimidines; D014498:Uracil / Q000378:metabolism; D051981:Uracil-DNA Glycosidase
D000470:Alkaloids; D004269:DNA, Bacterial; D004270:DNA, Circular; D004611:Ellipticines; D011687:Purines; D011743:Pyrimidines; C038373:9-aminoellipticine; D014498:Uracil; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase