Participation of c-myc protein in DNA synthesis of human cells.
The protein product of oncogene c-myc is believed to be important in regulation of the cell cycle. However, its direct role in DNA synthesis has not been explored. Experiments presented here show that the addition of affinity-purified antibodies against the human c-myc protein to nuclei isolated from several types of human cells reversibly inhibited DNA synthesis and DNA polymerase activity of these nuclei. This suggests that c-myc encodes a protein that is functionally involved in DNA synthesis.
D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology; D002474:Cell-Free System; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D006801:Humans; D007158:Immunologic Techniques; D019384:Nucleic Acid Synthesis Inhibitors; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D011519:Proto-Oncogenes
D019384:Nucleic Acid Synthesis Inhibitors; D011518:Proto-Oncogene Proteins; D004247:DNA