Analysis of inactive renin by renin profragment monoclonal antibodies.
Two peptides were synthesized, corresponding to the sequences (-19 to -7) and (-26 to -17) of the prorenin prosegment. Monoclonal antibodies were raised to these sequences and used to characterize human plasma inactive renin. Only anti (-19 to -7) reacted with inactive renin, as measured by direct assay or affinity chromatography. The data were used to evaluate two possible inactive renin stuctures: plasma inactive renin is a truncated prorenin lacking the prosegment N-terminal portion; its spatial conformation masks the N-terminal extremity, preventing interaction of this region with specific antibodies.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D002846:Chromatography, Affinity; D004792:Enzyme Precursors / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D011487:Protein Conformation; D012083:Renin / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D004792:Enzyme Precursors; D012083:Renin