Rapid and simple HPLC determination of 5,5-dimethyl-2,4-oxazolidynedione in rabbit serum.
A new method for the determination of 5,5-dimethyl-2,4-oxazolidynedione (DMO) in rabbit serum by reversed phase-HPLC with UV detection is described. The determination of DMO is performed without derivatisation. The internal standard is 5,5-diethylbarbituric acid (barbital). The method is rapid and simple with sensitivity limit of 20 ng/mL, high recovery (above 92%) and is suitable for pharmacokinetic studies.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004114:Dimethadione / Q000097:blood*; D010080:Oxazoles / Q000097:blood*; D011817:Rabbits
D010080:Oxazoles; D004114:Dimethadione