Simple determination of forphenicinol in human plasma and erythrocytes by HPLC with native fluorescence detection.
A high performance liquid chromatographic method is described for monitoring forphenicinol, a possible therapeutic drug for cancer and muscular dystrophy, in human plasma and erythrocytes. Forphenicinol in the deproteinized samples was separated from interfering biogenic substances on an aminopropyl-bonded silica (Unicil NH2) column within 10 minutes with isocratic elution, and determined with fluorescence detection. The detection limits for forphenicinol in plasma and erythrocytes are 65 pmol (12.8 ng)/ml and 160 pmol (31.5 ng)/ml, respectively, corresponding to 2 pmol each in a 100 microliters injection volume. The method is very simple, and sensitive enough to permit the quantification of forphenicinol in the blood samples from man dosed with forphenicinol.
D000276:Adjuvants, Immunologic / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000032:analysis*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D006801:Humans; D013050:Spectrometry, Fluorescence
D000276:Adjuvants, Immunologic; C035946:forphenicinol; D005998:Glycine