Genetic and molecular analyses of Escherichia coli N-acetylneuraminate lyase gene.
Two plasmids containing the N-acetylneuraminate lyase (NALase) gene (nanA) of Escherichia coli, pNL1 and pNL4, were constructed. Immunoprecipitation analysis indicated that the 35,000-dalton protein encoded in pNL4 was NALase. The synthesis of NALase in E. coli carrying these plasmids was constitutive.
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008970:Molecular Weight; D007652:Oxo-Acid-Lyases / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D012794:Sialic Acids / Q000378:metabolism
D012794:Sialic Acids; D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase