Gas chromatographic determination of the fatty acid composition of endotoxins from different bacteria.
Endotoxins from four bacterial species extracted by three different procedures were acid-methanolyzed and the methyl esters of the fatty acids were analyzed by packed-column gas chromatography. There were qualitative and quantitative differences in the fatty acid profiles of the lipopolysaccharides isolated from four Gram-negative bacteria. Our data show considerable lot-to-lot variations in amounts of four methyl esters from the same bacterial serotype extracted by the same procedure and in the same bacterial serotype extracted by different procedures. These results indicate that extraction and perhaps culture conditions, as well as bacterial species, affect the fatty acid composition of endotoxins, hydrolyzed and derivatized by these procedures.
D001419:Bacteria / Q000032:analysis*; D002849:Chromatography, Gas; D004731:Endotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D006868:Hydrolysis; D012475:Salmonella / Q000032:analysis; D012486:Salmonella typhimurium / Q000032:analysis; D012706:Serratia marcescens / Q000032:analysis
D004731:Endotoxins; D005227:Fatty Acids