Detection of anti-Lea in Le(a-b+) individuals by kinetic ELISA.
Utilizing a kinetic enzyme-linked antiglobulin binding assay, eight examples of anti-Lea were detected in individuals whose Lewis phenotype is Le(a-b+). These antibodies were not detectable by routine hemagglutination techniques, but were indistinguishable from anti-Lea made by Le(a-b-) individuals in terms of their immunologic fine specificity. However, unlike most anti-Lea antibodies from Le(a-b-) individuals, none of the anti-Lea antibodies had an IgG component. Anti-Lea antibodies in Le(a-b+) individuals may represent immune responses on the continuum between autoimmunity and alloimmunity.
D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000032:analysis*; D007700:Kinetics; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008297:Male
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies; D007983:Lewis Blood Group Antigens