Anti-TNP-forming cells in bronchus-associated lymphoid tissue (BALT) and paratracheal lymph node (PTLN) of the rat after intratracheal priming and boosting with TNP-KLH.
The aim of the present study was to elucidate the role of the paratracheal lymph node (PTLN) and bronchus-associated lymphoid tissue (BALT) in the generation of antigen-specific antibody-forming cells after intratracheal administration of trinitrophenyl-conjugated keyhole limpet haemocyanin (TNP-KLH). Priming as well as boosting resulted in the occurrence of specific antibody-forming cells in the PTLN, in BALT and in the lung. No anti-TNP-forming cells were observed in Peyer's patch, mesenteric lymph node and popliteal lymph node. From the data on the kinetics of anti-TNP-forming cells, it is concluded that intratracheal administration of antigen leads to a locally confined plasmacellular reaction. This suggests that the migrating and/or homing capacities of memory cells generated in the lung environment (PTLN, BALT) differ from those generated in the gut.
D000818:Animals; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D001980:Bronchi / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007124:Immunoenzyme Techniques; D008168:Lung / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology; D014132:Trachea / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D014301:Trinitrobenzenes; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin