title: A technique for repeated sampling of CSF from the anesthetized rat.
abstract: A method for repeatedly sampling cerebrospinal fluid (CSF) from anesthetized rats is described. The technique reliably and quickly yields blood-free samples of CSF and requires supplies that are commonly available. Samples as large as 250 microliter can be collected in a few minutes. There is no apparent malaise even when CSF is withdrawn once every three days for two weeks. This technique offers an alternative to surgical cannulation for sampling of CSF from the rat.
mesh_terms: D000768:Anesthesia, General; D000818:Animals; D002555:Cerebrospinal Fluid; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D013238:Stereotaxic Techniques