Anisomycin and cycloheximide, like growth factors, stimulate rapidly ATP turnover in 3T3 cells.
Addition of EGF and insulin to quiescent cultures of 3T3 cells induce a rapid stimulation of ATP turnover. We show that, like growth factors, anisomycin and cycloheximide, two inhibitors of protein synthesis, induce in 3T3 cells, a rapid increase in ATP turnover. However, this effect was not the result of the inhibition of protein synthesis since puromycin, in the same experimental conditions, did not stimulate ATP turnover.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D010710:Phosphates / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology*; D013997:Time Factors
D006133:Growth Substances; D007328:Insulin; D010710:Phosphates; D011759:Pyrrolidines; D011691:Puromycin; D004815:Epidermal Growth Factor; D000841:Anisomycin; D000255:Adenosine Triphosphate; D003513:Cycloheximide