title: Enzyme immunoassay for captopril.
abstract: A simple enzyme immunoassay for the determination of captopril was developed. A specific antibody for captopril was produced in rabbits that were immunized with a hapten-bovine immunoglobulin G conjugate, which was prepared by using 4-(maleimidomethyl)cyclohexane carboxylic acid as a spacer group. The limit of detection in plasma is 0.5 ng/mL. The assay has an adequate specificity, so isolation of captopril is unnecessary.
mesh_terms: D000818:Animals; D002216:Captopril / Q000032:analysis*; D003429:Cross Reactions; D005696:Galactosidases; D007124:Immunoenzyme Techniques; D011817:Rabbits; D013050:Spectrometry, Fluorescence
chemical_list: D002216:Captopril; D005696:Galactosidases