Specificity of antibodies to the purified Con A acceptor glycoproteins of cultured tumour cells.
Con A acceptor glycoproteins from the human Molt 4 (T cell leukaemia) and HeLa (endocervical adenocarcinoma) cell lines were purified by affinity chromatography and used for the preparation of rat antisera. Cross-absorption analysis showed that each antiserum contained antibodies which recognised cell surface antigens preferentially expressed by the donor cell line. Molt 4-associated antigens were fully expressed on T cell tumour lines and normal thymocytes, but not on non T cell tumour lines, peripheral blood lymphocytes or other blood cells. Immunofluorescence studies showed that the antigens were preferentially expressed on a sub-population of immature thymocytes. HeLa-associated antigens were only fully expressed on one other epithelial tumour cell in a panel of 17 cell lines. Immunofluorescence studies showed that the HeLa-associated antigens were expressed on normal endocervical adenoepithelium but not on ectocervical, endometrial or intestinal epithelia. Thus purified Con A acceptor glycoproteins of cultured tumour cell lines are potent immunogens for the generation of antibodies recognising lineage-associated differentiation antigens. These antigens should be useful in tumour classification and in the study of normal differentiation.
D000230:Adenocarcinoma / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D002584:Cervix Uteri / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D007938:Leukemia / Q000276:immunology*; D011952:Receptors, Concanavalin A / Q000276:immunology* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000954:Antigens, Surface; D006023:Glycoproteins; D011952:Receptors, Concanavalin A; C027774:concanavalin A-binding glycoproteins