title: Acid phosphatase activity in mononuclear phagocytes and the U937 cell line: monocyte-derived macrophages express tartrate-resistant acid phosphatase.
abstract: Tartrate-resistant acid phosphatase (TRAcP) is used as a marker for osteoclasts, which are believed to be derived from phagocytic cells or phagocyte stem cell precursors. To further investigate the relationship between monocytic phagocytes and osteoclasts, acid phosphatase (AcP) activity was measured by three different techniques in human peripheral blood monocytes, monocyte-derived macrophages, and the U937 cell line. We found that cytochemistry and gel electrophoresis led to similar results, but that the colorimetric assay was inconsistent. Normal human peripheral monocytes expressed both tartrate-sensitive and -resistant AcP. In culture these cells formed polykaryons and expressed TRAcP activity that was further identified as an isoenzyme associated with bone tissue. In contrast, the U937 cells did not express TRAcP activity as measured by gel electrophoresis. Both U937 cells and monocytes possess material that interferes with interpretation of the colorimetric assay of AcP. The presence of TRAcP in monocyte-derived macrophages further supports the relationship between phagocytic cells and bone osteoclasts.
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D002117:Calcitriol / Q000494:pharmacology; D002460:Cell Line; D003124:Colorimetry; D006651:Histocytochemistry; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D016403:Lymphoma, Large B-Cell, Diffuse / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology*; D009000:Monocytes / Q000201:enzymology*; D013644:Tartrates / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D013644:Tartrates; D000135:Acid Phosphatase; D002117:Calcitriol; C029768:tartaric acid