In vitro damage to rat lens by lumazine and xanthine oxidase: prevention by superoxide dismutase.
Intact rat lenses incubated with lumazine and xanthine oxidase are physiologically damaged as evidenced by a decrease in the net accumulation of rubidium ions against a concentration gradient. Superoxide dismutase protected the tissue against this damage. These experiments, therefore, demonstrate the susceptibility of the lens tissue to O2- injury under ambient and nonphotochemical conditions, suggesting a possible implication of this radical in the tissue in vivo and eventual cataract formation. The lumazine/xanthine oxidase system which is known to cause oxygen reduction predominantly by the monovalent route, producing superoxide, appears quite suitable to evaluate the toxicity of O2- to the tissues in vitro.
D000818:Animals; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000187:drug effects* / Q000473:pathology; D010084:Oxidation-Reduction; D011621:Pteridines / Q000494:pharmacology*; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000494:pharmacology*
D005609:Free Radicals; D011621:Pteridines; C008297:lumazine; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase