A new type of methylamine oxidase: the sole oxidase produced during growth of Sporobolomyces albo-rubescens on primary alkylamines.
Under conditions known to separate methylamine oxidase from benzylamine oxidase in other yeast strains, only a single oxidase could be detected in Sporobolomyces albo-rubescens. This occurred irrespective of whether methylamine or n-butylamine was the nitrogen source for growth. The oxidase did not attack benzylamine. It was concluded that this organism can only produce a methylamine oxidase. The enzyme was purified to 90% homogeneity and found to have properties significantly different from the methylamine oxidases previously characterised. It lost only 40% of its activity in 30 min at 45 degrees C, whereas methylamine oxidases previously described had half-lives of from 2 to 9 min at 45 degrees C. It showed also a lower activity with short chain 1-aminoalkanes and a higher activity with longer chain 1-aminoalkanes than other methylamine oxidases, and had a significantly smaller subunit molecular weight (57,000 compared with 80,000).
D000473:Alkanes / Q000378:metabolism; D000588:Amines / Q000378:metabolism; D002082:Butylamines / Q000378:metabolism*; D007700:Kinetics; D008744:Methylamines / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000378:metabolism*; D008970:Molecular Weight; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000096:biosynthesis* / Q000302:isolation & purification; D013379:Substrate Specificity; D013696:Temperature
D000473:Alkanes; D000588:Amines; D002082:Butylamines; D008744:Methylamines; C027451:methylamine; C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; C032462:n-butylamine