Effects of glucose and fructose added to the intraocular irrigating solutions on the rabbit ERG in vitro.
The addition of various concentrations of glucose to intraocular irrigating solutions for the retina was studied electrophysiologically using an in vitro eye-cup in the pigmented rabbit. Comparison of b-wave amplitudes demonstrated that, glucose at a concentration of 20 mM/l yielded the highest values. With this concentration, the b-wave amplitude was markedly enhanced to 250% at 90 minutes. With 5 mM/l glucose (normogycemic) solutions, the b-waves were decreased to below 20% at 60 minutes. A 40 mM/l glucose solution also increased the b-waves but to a lesser extent than 20 mM/l. These results might be due to the detrimental effects of osmolarity. However, under hypoxic conditions the amplitudes of b-waves obtained with 40 mM/l glucose solutions were greater than those with 20 mM/l glucose solutions. The addition of 10 mM/l fructose to the control solution had no remarkable effects suggesting that fructose is not a direct energy source for the in vitro retina.
D000818:Animals; D004594:Electrophysiology; D004596:Electroretinography; D005632:Fructose / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D009883:Ophthalmic Solutions / Q000008:administration & dosage*; D009994:Osmolar Concentration; D011817:Rabbits; D012160:Retina / Q000502:physiology*; D007507:Therapeutic Irrigation; D013997:Time Factors
D009883:Ophthalmic Solutions; D005632:Fructose; D005947:Glucose