Lack of response of rat chondrosarcoma chondrocytes to interleukin-1 to produce proteoglycan- and collagen-degrading enzymes.
Chondrocytes obtained from rat articular cartilage and Swarm rat chondrosarcoma were maintained in culture. Human interleukin-1 (IL-1) was added at confluency, and three days later the spent media was evaluated for proteoglycan- and collagen-degrading enzymes. Unlike chondrocytes from articular cartilage from several species, chondrocytes from Swarm rat chondrosarcoma did not respond to IL-1 stimulation. This lack of sensitivity to IL-1 may be a useful biochemical marker to classify various types of chondrosarcomas. However, it appears that the use of chondrosarcoma chondrocyte cultures are not useful as a source for metalloproteinases.
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000201:enzymology*; D002460:Cell Line; D002478:Cells, Cultured; D002813:Chondrosarcoma / Q000201:enzymology*; D003094:Collagen / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000302:isolation & purification / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits; D051381:Rats
D007375:Interleukin-1; D011509:Proteoglycans; D003094:Collagen; D010447:Peptide Hydrolases