title: T lymphocyte population dynamics and function following a primary bovine herpesvirus type-1 infection.
abstract: Following a primary bovine herpesvirus-1 (BHV-1) infection the concanavalin A (Con A) induced proliferative responses of peripheral blood T lymphocytes were suppressed. This suppression occurred in the absence of detectible serum suppressor factors, suppressor cell activity or decreased accessory cell function. However, regression analysis demonstrated a significant correlation between the percentage of T lymphocytes present within the peripheral blood mononuclear cell (PBMC) population and the amplitude of Con-A-induced lymphocyte proliferative responses (LPR). Direct evidence that a numerical deficit of responder T lymphocytes was limiting LPR was obtained by using an immunomagnetic microsphere (IMM) negative enrichment protocol to produce a PBMC population with a constant percentage (75 +/- 6%) of T lymphocytes. The Con-A-induced LPR of these enriched T lymphocytes remained constant following BHV-1 infection. Flow cytometric (FC) analysis of PBMC indicated that the decreased percentage of circulating T lymphocytes, associated with BHV-1 infection, was caused primarily by a selective depletion of the BoT8 subset. These FC data were consistent with the indirect evidence of increased TH activity, as indicated by elevated Con A-induced IL-2 production. Thus, 2 to 5 days following viral infection, the circulating T lymphocytes were activated as shown by elevated IL-2 production, increased recombinant bovine IL-2 (rBo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001831:Body Temperature; D002417:Cattle; D002469:Cell Separation; D003208:Concanavalin A / Q000378:metabolism; D005434:Flow Cytometry; D007132:Immunoglobulin Isotypes / Q000032:analysis; D007165:Immunosuppression Therapy; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology*; D007376:Interleukin-2 / Q000032:analysis; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D007376:Interleukin-2; D003208:Concanavalin A