Localization of a determinant for HEp-2 adherence by enteropathogenic Escherichia coli.
pMAR2, a 60-megadalton plasmid encoding localized HEp-2 adherence in enteropathogenic Escherichia coli, was mapped with BamHI, HindIII, and SalI. Deletion and insertion mutants were constructed and used to define a potential DNA probe. Preliminary results indicate that this probe is sensitive and specific for the genes encoding the enteropathogenic E. coli adherence factor.
D000268:Adhesiveness; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D005798:Genes, Bacterial; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids