Growth of human oligodendrocytes in vitro on extracellular matrix.
Based on the successful use of a basement membrane-like extracellular matrix (ECM) as a physiological substrate for the growth of adult rat oligodendrocytes, we have applied the system to oligodendroglia of human origin. ECM is excreted and deposited on a culture dish by endothelia cells. Glial cells isolated from fresh human brains by a Percoll density gradient were maintained on ECM for several weeks in vitro. Cells identified as oligodendrocytes by anti-galactocerebroside (GalC) could be transferred and replated on ECM-coated plates. In one case, GalC-positive cells isolated from a biopsy of an 8-year old girl were able to incorporate [3H]thymidine as revealed by autoradiography. ECM can be used successfully for the growth and in vitro maintenance of human oligodendrocytes.
D001345:Autoradiography; D002478:Cells, Cultured; D005109:Extracellular Matrix; D005455:Fluorescent Antibody Technique; D005699:Galactosylceramides / Q000378:metabolism; D006801:Humans; D009457:Neuroglia; D009836:Oligodendroglia; D013936:Thymidine / Q000378:metabolism
D005699:Galactosylceramides; C002454:galactocerebroside; D013936:Thymidine