Thrombospondin in essential thrombocythemia.
Essential thrombocythemia is a myeloproliferative disorder characterized by frequent bleeding and thrombotic complications. On a molecular level, two abnormalities of platelet thrombospondin have been identified: abnormal glycosylation of the intact 185,000-dalton chain has been detected and a shortened form of the thrombospondin chain is present. We have used two monoclonal antibodies and Lens culinaris lectin to probe the structure of thrombospondin in the platelets from three patients with essential thrombocythemia; one patient with polycythemia vera and two patients with secondary thrombocytosis. The presence of abnormal thrombospondin fragments with molecular weights of 160,000 and 30,000 was detected in the intact platelets and in the supernatant from thrombin-treated platelets, in all of the individuals except one of the secondary thrombocytosis patients. Monoclonal antibody binding studies indicate that both fragments are produced by proteolysis at a single site, which results in the removal of a 30,000-dalton fragment from the NH2-terminal. Lens culinaris lectin-binding studies revealed that some of the carbohydrate moieties of thrombospondin are near this cleavage site. The results are consistent with the hypothesis that the abnormal thrombospondin fragments observed under conditions of increased platelet production are due to increased susceptibility to proteolysis which, in turn, may be due to defective glycosylation.
D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D037102:Lectins; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases / Q000378:metabolism; D037121:Plant Lectins; D013920:Thrombocythemia, Essential / Q000097:blood*; D019699:Thrombospondins
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D037102:Lectins; D010446:Peptide Fragments; D037121:Plant Lectins; D019699:Thrombospondins; C033619:lentil lectin; D010447:Peptide Hydrolases