title: Antibodies isolated by using cloned surface antigens recognize antigenically related components of Legionella pneumophila and other Legionella species.
abstract: We studied the antigenic cross-reactivity of surface proteins among various strains of Legionella pneumophila and other Legionella species by using a novel method of antibody purification. Anti-bacterial antibodies in hyperimmune sera were adsorbed to and eluted from the surface of recombinant E. coli cells that express individual L. pneumophila antigens on their surface. These affinity-purified antibodies were then used to probe protein immunoblots prepared from the test strains to detect cross-reactive domains. We found that antigenic proteins are generally conserved in all L. pneumophila serogroups. Although some of these antigenic domains are shared with members of other Legionella species, they are associated with proteins of different molecular mass. Our approach to the study of antigenic cross-reactivity has potential advantages over similar studies that use either monoclonal antibodies or monospecific antibodies prepared by immunization with purified antigens.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D003001:Cloning, Molecular; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D007875:Legionella / Q000145:classification / Q000276:immunology*; D008970:Molecular Weight; D010209:Paper; D011817:Rabbits; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D003101:Collodion