A semiquantitative probe for radiation-induced normal tissue damage at the molecular level.
Sheep antibodies to bovine type I collagen were employed in the immunohistochemical detection of type I collagen in lung tissue sections of irradiated LAF1 mice. A video image digitizing system was developed to estimate collagen levels, by assigning a numerical value (0-63) to each of approximately 53,800 picture elements (pixels) in the microscope field, according to the collagen-dependent fluorescence intensity at each locus. For lungs harvested 52 weeks subsequent to graded doses of 60Co gamma radiation between 0 and 10 Gy, a dose-dependent increase in type I collagen was observed in the alveolar walls. A reproducible increase was evident for doses as low as 5 Gy: doses of 7 to 10 Gy elicited type I collagen levels significantly elevated with respect to those of age-matched controls. These results are consistent with a role for type I collagen in the development of radiation-induced pulmonary fibrosis. The assay system developed here will be used to explore the role of connective tissue macromolecules in the development of radiation pneumonitis and fibrosis.
D000818:Animals; D003037:Cobalt Radioisotopes; D003094:Collagen / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005720:Gamma Rays; D008168:Lung / Q000528:radiation effects; D008297:Male; D051379:Mice; D011658:Pulmonary Fibrosis / Q000209:etiology / Q000473:pathology; D011832:Radiation Injuries / Q000473:pathology*
D003037:Cobalt Radioisotopes; D003094:Collagen