Decreased c-myc expression is an early event in retinoic acid-induced differentiation of F9 teratocarcinoma cells.
Retinoic acid-induced differentiation of mouse F9 teratocarcinoma cells is known to be accompanied by changes in gene expression. We examined the expression of the c-myc gene during retinoic acid-induced differentiation of F9 cells. Retinoic acid caused a 50% reduction in the level of c-myc mRNA after 3 hr of treatment and a 90% reduction after 12 hr. We have also examined several features of c-myc genomic structure in F9 cells, PYS2 (differentiated parietal yolk sac) cells, and liver--namely, methylation, amplification, and gross organization. Comparison of Hpa II and Msp I digests of DNAs from F9, PYS2, and liver showed that the c-myc gene in F9 cells is hypomethylated relative to that in PYS2 cells and in liver. The Hpa II sites that undergo methylation during differentiation were found to be in the second intron. Gross structural changes of the c-myc gene have not occurred in F9 or PYS2, and the c-myc gene is not amplified.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D005784:Gene Amplification; D005786:Gene Expression Regulation / Q000187:drug effects*; D008745:Methylation; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D013724:Teratoma / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology*
D011518:Proto-Oncogene Proteins; D014212:Tretinoin