Characterization of cadmium-binding proteins detected in rat liver by the western blotting technique.
Out of the three cadmium-binding proteins (CD-BPs) in rat liver parenchyma (40K, 29K, and 24K CdBPs), the 40K Cd-BP showed the highest affinity for cadmium (Cd), with a dissociation constant (KD) of 1.2 x 10(-8) M. This is in between the affinity of human serum albumin KD = 3.8 x 10(-5) M) and metallothionein (KD = less than 10(-11)). These Cd-BPs may be responsible for hepatic sequestration of cadmium.
D000818:Animals; D015153:Blotting, Western; D002104:Cadmium / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008297:Male; D008668:Metallothionein / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
C004203:cadmium-binding protein; D002104:Cadmium; D008668:Metallothionein