High-performance liquid chromatographic (HPLC) assay using fluorescence detection for the simultaneous determination of gallopamil and norgallopamil in human plasma.
Gallopamil is a calcium-channel antagonist with reported activity in experimental animals three to five times higher than that of verapamil. An automated high-performance liquid chromatographic (HPLC) method with fluorescence detection is described for the simultaneous determination of gallopamil and its metabolite norgallopamil in plasma. Gallopamil was well resolved from norgallopamil and other metabolites, allowing simultaneous quantitation of both drugs. The detection limit for both gallopamil and norgallopamil was 0.9 ng/ml. This method has been successfully used for the determination of gallopamil and norgallopamil following the administration of 25-, 37.5-, and 50-mg oral doses of drug.
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D005711:Gallopamil / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
C052471:norgallopamil; D005711:Gallopamil