NADPH-induced microsomal lipid peroxidation as measured by malondialdehyde production in rat liver. Inhibitory effect of naftidrofuryl.
The effects of naftidrofuryl have been studied on NADPH-induced microsomal lipid peroxidation by measuring malondialdehyde (MDA) production. Conditions adequate to measure MDA production and effects of naftidrofuryl on MDA production have been tested. It has been shown that the addition of ferric ions is essential with Tris or Pipes buffers while it can be omitted with phosphate known to contain traces of ferric ions. However the initial rate of MDA production is much lower with phosphate in the absence of added ferric ions, showing that the initiation of lipid peroxidation is limited by ferric ions. The effects of naftidrofuryl have been studied on MDA production in phosphate buffer in the presence or absence of ferric ions. Naftidrofuryl inhibits lipid peroxidation in both conditions indicating that the inhibition is not related to an interaction with added ferric ions. Naftidrofuryl is efficient at concentrations slightly higher than butylhydroxytoluene but lower than aminopyrine.
D000818:Animals; D000975:Antioxidants; D002021:Buffers; D005663:Furans / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008315:Malondialdehyde / Q000096:biosynthesis; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D009257:Nafronyl / Q000494:pharmacology*; D051381:Rats
D000975:Antioxidants; D002021:Buffers; D005663:Furans; D008054:Lipid Peroxides; D009257:Nafronyl; D008315:Malondialdehyde; D009249:NADP; D007501:Iron