title: The effects of epidermal growth factor on neural crest cells in tissue culture.
abstract: Epidermal growth factor (EGF) stimulates the release of hyaluronic acid (HA) and chondroitin sulfate proteoglycan (CSPG) from quail trunk neural crest cultures in a dose-dependent fashion. It also promotes the expression of cell-associated heparan sulfate proteoglycan (HSPG) as detected by immunofluorescence and immunoprecipitation of the 3H-labeled proteoglycan. Furthermore, EGF stimulates [3H]thymidine incorporation into total cell DNA. These results raise the possibility that EGF or an analogous growth factor is involved in regulation of neural crest cell morphogenesis.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003370:Coturnix; D004261:DNA Replication / Q000187:drug effects; D004625:Embryo, Nonmammalian; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans; D004815:Epidermal Growth Factor; D006820:Hyaluronic Acid; D066246:ErbB Receptors; D005944:Glucosamine