Free radical chain oxidation and hemolysis of erythrocytes by molecular oxygen and their inhibition by vitamin E.
Erythrocytes of vitamin E-deficient rats and normal rats were oxidized at 37 degrees C by molecular oxygen using a free radical initiator. The erythrocytes were oxidized by a free radical chain mechanism with kinetic chain length considerably larger than 1 and resulted in hemolysis. Vitamin E suppressed both oxidation and hemolysis, but the extent of hemolysis was determined primarily by the extent of oxidation independent of the presence or absence of vitamin E.
D000578:Amidines; D000818:Animals; D000975:Antioxidants; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005609:Free Radicals; D006461:Hemolysis / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014810:Vitamin E / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000097:blood
D000578:Amidines; D000975:Antioxidants; D005609:Free Radicals; D014810:Vitamin E; C046728:2,2'-azobis(2-amidinopropane); D010100:Oxygen