Abnormalities of the ear associated with exencephaly in mouse fetuses induced by maternal exposure to cadmium.
Exencephaly was induced in mouse fetuses by maternal injection of cadmium chloride (CdCl2) on day 7 of gestation. The heads of exencephalic, nonexencephalic experimental, and control fetuses were embedded in paraffin and sections were stained with hematoxylin and eosin. Compared to those of controls, the ears of the exencephalic fetuses were smaller (microtia) and low set. The meatal plug representing the external auditory canal was thick, variously branched, and often directed inferiorly. Usually, there were just two ossicles. The stapedial artery, facial nerve, and stapedius muscle were hypoplastic; the tensor tympani was small or absent. There were 1.0 to 2.0 turns of the cochlea in contrast to 2.5 turns in the controls. The organ of Corti was underdifferentiated; the spiral ganglion had fewer cells. In the control, the long axes of the anterior and posterior semicircular ducts were at right angles to each other and in vertical planes, but in the exencephalics, they tended more laterally towards the horizontal plane. The differentiation of the cristae ampullares and maculae was also severely affected. In several specimens, the entire membranous labyrinth had been distended; these labyrinths also had unusual epithelial infoldings. In cadmium-treated nonexencephalic fetuses, the external ears were normal and appropriate to the body size; five of them were examined histologically; in all, the five middle ear contents were hypoplastic; in three, the cochlea had a maximum of two turns and the organ of Corti, crista ampullaris, and macula were hypoplastic. By an analogy to abnormalities of mutants with neural tube defects, it is suggested that the exencephaly induced by cadmium might affect the differentiation of the ear. Partial involvement of the ear in nonexencephalic experimental embryos may be the result of direct action of cadmium during critical stages of development.
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001921:Brain / Q000002:abnormalities*; D002104:Cadmium / Q000633:toxicity*; D004423:Ear / Q000002:abnormalities*; D004431:Ear, External / Q000002:abnormalities / Q000473:pathology; D007758:Ear, Inner / Q000002:abnormalities / Q000473:pathology; D004432:Ear, Middle / Q000002:abnormalities / Q000473:pathology; D005260:Female; D005865:Gestational Age; D008431:Maternal-Fetal Exchange; D051379:Mice; D011247:Pregnancy
D002104:Cadmium