Synthesis and distribution of laminin-related polypeptides in early amphibian embryos.
Western blotting experiments carried out with several heterospecific antibodies against mouse-derived laminin allowed the identification of four laminin-related polypeptides in early Pleurodeles waltlii embryos. Synthesis of all four polypeptides was detected from the early blastula stage to late gastrula stage. Immunofluorescent staining with anti-laminin and anti-fibronectin antibodies provided evidence for a close association of these laminin-related polypeptides with the fibronectin fibrillar network.
D000818:Animals; D000911:Antibodies, Monoclonal; D001755:Blastocyst / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D005455:Fluorescent Antibody Technique; D005775:Gastrula / Q000378:metabolism; D007797:Laminin / Q000032:analysis / Q000096:biosynthesis*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D010999:Pleurodeles
D000911:Antibodies, Monoclonal; D007797:Laminin; D010455:Peptides