Plasmodium falciparum: S-adenosyl-L-methionine decarboxylase.
Putrescine-dependent S-adenosyl-L-methionine decarboxylase has been detected in the malaria parasite Plasmodium falciparum. Mg2+ did not affect the enzyme activity. The apparent Km value of the plasmodial enzyme for adenosyl-methionine was found to be 33 microM. Methylglyoxal bis(guanylhydrazone) competitively inhibited the enzyme activity with respect to adenosylmethionine. The inhibition constant for methylglyoxal bis(guanylhydrazone) was determined to be 0.46 microM. Spermidine was the main polyamine detected in the parasite. There was significant decrease in the S-adenosyl-L-methionine decarboxylase activity when the infected erythrocytes were incubated with chloroquine and mefloquine for 2 hr at 1 and 10 microM, respectively. Since at similar concentrations these drugs did not directly affect the plasmodial enzyme activity, the interaction of these drugs with the polyamine biosynthesis remains unclear.
D012437:Adenosylmethionine Decarboxylase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002738:Chloroquine / Q000494:pharmacology; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015767:Mefloquine; D008935:Mitoguazone / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000032:analysis / Q000187:drug effects / Q000201:enzymology*; D011073:Polyamines / Q000032:analysis; D011700:Putrescine / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology
D011073:Polyamines; D011804:Quinolines; D002738:Chloroquine; D002262:Carboxy-Lyases; D012437:Adenosylmethionine Decarboxylase; D008935:Mitoguazone; D015767:Mefloquine; D011700:Putrescine