[A new vector for cloning of genes and replicons in yeasts].
A novel Escherichia coli-Saccharomyces cerevisiae shuttle vector lambda MAN78 has been constructed. The vector contains phage lambda 47.1 DNA, Sacch. cerevisiae chromosomal segment with TRP1 gene and the yeast ARS1 replicator. This vector may be propagated as a phage and, similar to parental lambda 47.1, allows direct selection of large DNA inserts (15-24 kbp) in E. coli. lambda MAN78 can efficiently transform LiCl-treated yeast cells (3-5 X 10(3) transformants per 1 microgram DNA). Replication of hybrid molecules in E. coli cells does not influence the ability of the molecules to transform yeast cells and replicate in the cells.
D003001:Cloning, Molecular; D005800:Genes, Fungal; D005822:Genetic Vectors; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014169:Transformation, Bacterial
Novyĭ vektor dlia klonirovaniia genov i replikonov v drozhzhakh.