Characterization of a 19,000 mol. wt. flagellum-specific protein of Trypanosoma cruzi, T. dionisii and T. vespertilionis by a monoclonal antibody.
The flagellum-specific monoclonal antibody VESP 9.1 raised against Trypanosoma vespertilionis reacts with a single protein band of 19,000 mol. wt.. This antigen is present on the flagellum of T. cruzi, T. dionisii and T. vespertilionis as well on the epimastigote and the metacyclic trypomastigote developmental stage. A comparative study using indirect immunofluorescence with 15 different trypanosomatids confirmed the specific reactions.
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis*; D005407:Flagella / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D008970:Molecular Weight; D015800:Protozoan Proteins; D013045:Species Specificity; D014345:Trypanosoma / Q000032:analysis*; D014349:Trypanosoma cruzi / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C052356:flagellum-specific antigen, Trypanosoma