Pleiotropic changes in cycloheximide-resistant insect cell clones.
Somatic cell mutants resistant to drugs that interact with the eukaryotic ribosome provide a useful tool for studies on ribosome structure, function, and genetics. From Aedes albopictus (mosquito) cells, cycloheximide-resistant mutants (Cx-705 and Cx-738) that were about 30-fold more resistant to cycloheximide than the parental cells have been obtained. The observation that protein synthesis in cell-free lysates from Cx-705 and Cx-738 cells was resistant to cycloheximide led us to suspect that the alteration in these mutants might affect the ribosome. The present studies show that the cycloheximide-resistant cells grow poorly and eventually die at 34.5 degrees C, a temperature at which wild-type cells grow normally. Relative to control cells, the cycloheximide-resistant cells show increased sensitivity to G-418, another antibiotic that interacts with the eukaryotic ribosome. However, there were no differences between cycloheximide-resistant cells and wild-type cells in sensitivity to puromycin, emetine, or cryptopleurine. Cx-705 cells were predominantly diploid; in contrast, the frequency of tetraploid nuclei in Cx-738 cells was about 40%.
D000330:Aedes; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D002999:Clone Cells; D003513:Cycloheximide / Q000494:pharmacology*; D004351:Drug Resistance; D005839:Gentamicins / Q000494:pharmacology; D007621:Karyotyping; D009154:Mutation; D012269:Ribosomal Proteins / Q000032:analysis*; D013696:Temperature
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D012269:Ribosomal Proteins; D003513:Cycloheximide; C010680:antibiotic G 418