[Use of antibiotics in selecting Escherichia coli as a producer of penicillin acylase].
The effect of neomycin, streptomycin, chloramphenicol and erythromycin on survival of E. coli producing penicillinacylase and its variation with respect to the property of penicillinacylase production was studied. Neomycin and chloramphenicol had the highest lethal effect on the cells of E. coli. It was shown that neomycin, erythromycin and chloramphenicol, as selective factors, promoted detection of variants with the activity levels lower than the control ones. Streptomycin promoted detection of low active and high active variants with the same frequency.
D000581:Amidohydrolases / Q000096:biosynthesis*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D014644:Genetic Variation / Q000187:drug effects; D010399:Penicillin Amidase / Q000096:biosynthesis*; D012641:Selection, Genetic
D000900:Anti-Bacterial Agents; D000581:Amidohydrolases; D010399:Penicillin Amidase
Ispol'zovanie antibiotikov v selektsii Escherichia coli--produtsent penitsillinatsilazy.