Total protection from hypoxic liver damage by fructose.
The purpose of these experiments was to evaluate the effect of carbohydrates on hypoxic cell death in the perfused rat liver. Lactate dehydrogenase (LDH) was released maximally at rates up to 300 U/g/h after 40 to 60 min of hypoxia in control livers from fasted rats or in livers perfused with glucose (20 mM). Fructose (20 mM) prevented the release of LDH completely. Rates of anaerobic glycolysis indexed by release of lactate were around 150 mumol/g/h in livers perfused with fructose but were undetectable in livers perfused with glucose. The results demonstrate that fructose prevents hypoxic damage in the liver presumably by providing glycolytic ATP in the absence of oxygen.
D000693:Anaerobiosis; D000818:Animals; D005260:Female; D005632:Fructose / Q000494:pharmacology / Q000627:therapeutic use*; D006019:Glycolysis / Q000187:drug effects; D000860:Hypoxia / Q000378:metabolism / Q000517:prevention & control*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010477:Perfusion; D051381:Rats
D005632:Fructose; D007770:L-Lactate Dehydrogenase