Production of transgenic fish: introduction and expression of chicken delta-crystallin gene in medaka embryos.
To produce a model of transgenic fish, recombinant plasmids containing chicken delta-crystallin gene were microinjected into the oocyte nucleus of a small teleost, medaka (Oryzias latipes). About 50% of the microinjected oocytes developed to 7-day-old embryos. By Southern blotting delta-crystallin gene was detected in 4 of 8 embryos, and, by Western blotting, delta-crystallin polypeptides in 5 of 16. In 1 of 6 examined histologically, delta-crystallin DNA was detected in all the tissues, and delta-crystallin polypeptides, in many of the tissues including the lens. Thus, the exogenous gene and/or its products were detected in 10 of 30 embryos examined. This is the first report of successful production of transgenic fish.
D000818:Animals; D002645:Chickens / Q000235:genetics*; D003459:Crystallins / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism; D005399:Fishes / Q000235:genetics*; D005786:Gene Expression Regulation; D007163:Immunosorbent Techniques; D008845:Microinjections; D009865:Oocytes; D010957:Plasmids
D003459:Crystallins; D004274:DNA, Recombinant