Semi-preparative high-performance reversed-phase displacement chromatography of insulins.
A reversed-phase displacement chromatographic method has been developed for the purification of bovine and porcine insulin samples. Up to 500 mg of raw insulin could be purified on a Nucleosil C8 analytical column using a methanol-containing phosphate buffer carrier and a cetrimide-containing displacer. A proinsulin contamination level as low as 100 ppm in the collected fractions could be achieved.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007328:Insulin / Q000032:analysis*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D007328:Insulin