Increased adenylate energy charges in rat liver or primary cultured rat hepatocytes by diisopropyl 1,3-dithiol-2-ylidenemalonate.
Six hours after oral administration in rat liver, diisopropyl 1,3-dithiol-2-ylidenemalonate (malotilate) decreased AMP concentrations by 60% with unchanged total adenine nucleotide concentrations and significantly increased adenylate energy charges of 0.87 in comparison to those of 0.81 in vehicle administered control animals. Twelve hours after incubation in primary cultured rat hepatocytes, malotilate decreased AMP concentrations by 18% and increased ATP and GTP concentrations by 13 and 18% respectively with significantly increased adenylate energy charges of 0.89 in comparison to 0.87 in vehicle control. Increased adenylate energy charges by malotilate coincided with the initiation of increases in protein and de novo purine synthesis in vivo, and are associated with the increased rate of de novo purine synthesis in vitro.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D006150:Guanine Nucleotides / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008314:Malonates / Q000494:pharmacology*; D011685:Purine Nucleotides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012265:Ribonucleotides / Q000378:metabolism*
D000227:Adenine Nucleotides; D006150:Guanine Nucleotides; D008314:Malonates; D011685:Purine Nucleotides; D012265:Ribonucleotides; C024808:diisopropyl 1,3-dithiol-2-ylidenemalonate