In vitro growth of mastitis pathogens in mammary secretions of the dry and peripartum periods.
In vitro growth of Escherichia coli, Streptococcus uberis, and Staphylococcus aureus was measured in skimmed, cell-free secretions of bovine mammary glands collected on d 0, 7, 14, and 28 of the dry period; 12, 8, and 4 d before expected calving; and on d 0 and 14 postpartum. There were marked and nearly inverse changes in growth of Escherichia coli and Streptococcus uberis over the experimental period. Escherichia coli grew well in secretions of d 0 of the dry period and d 0 and 14 postcalving but poorly or not at all in secretions of the mid-dry period. In contrast, Streptococcus uberis grew well in secretions of the involuted gland and poorly in samples from day of dry off and day of calving. Growth of Staphylococcus aureus changed relatively little over the dry period, but there was reduction in growth at d 7 of the dry period. Changing ability of the secretion to support bacterial growth may influence changing susceptibility to infection, but for Streptococcus uberis, poor correspondence between changes in growth supported by the secretion and changes in the incidence of naturally occurring infection over the dry period indicates that other factors may be more important.
D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000254:growth & development*; D005260:Female; D066298:In Vitro Techniques; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000254:growth & development*; D013291:Streptococcus / Q000254:growth & development*