title: Immunological detection of an analogue of the erythroid protein 4.1 in endothelial cells.
abstract: Endothelial cells (EC) of arterial and venous origin were investigated by indirect immunofluorescence and immunoautoradiography for the presence of red cell membrane 4.1-like protein. By immunofluorescence, EC exhibited a relatively uniform fluorescent staining sometimes of a reticular pattern, distributed over the entire cell. All controls were negative. Immunoblot analysis of EC revealed a cross reactive band of a molecular weight comparable to that of the erythrocyte band 4.1. These findings indicate that endothelial cells of arterial and venous origin express a polypeptide immunologically related to the erythrocyte protein 4.1, which may play an important role in membrane-cytoskeleton interactions.
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D001798:Blood Proteins / Q000032:analysis*; D002417:Cattle; D003598:Cytoskeletal Proteins; D004727:Endothelium / Q000166:cytology*; D004910:Erythrocyte Membrane / Q000032:analysis; D005263:Femoral Artery / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008565:Membrane Proteins; D008970:Molecular Weight; D009479:Neuropeptides; D011863:Radioimmunoassay; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1