Allogeneic transplantation of normal epidermal cells and squamous cell carcinomas in SENCAR mice.
A method used in our laboratory for the transplantation of single cell suspensions of epidermis and skin carcinomas is described. Silicone chambers were placed into a subcutaneous granulation tissue formed by the implantation of a glass disk. The skin was closed on top of the chamber by using Michael autoclips. Cells were injected 24 to 48 hr later through the skin using a syringe and needle. The neoformation of epithelia with adnexa was observed when newborn epidermal cells were injected. When a fresh cell suspension of squamous cell carcinoma was used, a typical differentiated carcinoma was formed within 2 to 4 weeks. However, after 4 weeks, some of the grafts showed mild signs of rejection. The technique described is a useful system to transplant squamous cell carcinomas and can also be used as a rapid assay for malignancy.
D000818:Animals; D000831:Animals, Newborn; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004848:Epithelium / Q000033:anatomy & histology; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D012867:Skin / Q000033:anatomy & histology; D012878:Skin Neoplasms / Q000473:pathology*; D016038:Skin Transplantation; D014184:Transplantation, Homologous