Two forms of phosphatidylinositol-specific phospholipase C from bovine brain.
Two immunologically distinct forms of phosphoinositide-specific phospholipase C were purified to near homogeneity from bovine brain. Their molecular weights determined by SDS-PAGE are 150,000 (enzyme I) and 145,000 (enzyme II), respectively. Under a nondenaturing condition, purified enzyme I exists mainly in dimeric form and as tetramer to a small extent, while enzyme II is predominantly in monomer, to a small extent as dimer and to a very small extent as trimer. Multiple forms of phosphoinositide-specific phospholipase C in brain tissue described in the literature might be, therefore attributed to the oligomerization of the two independent forms.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D010716:Phosphatidylinositols / Q000378:metabolism*; D010738:Type C Phospholipases / Q000276:immunology / Q000302:isolation & purification*
D010716:Phosphatidylinositols; D010738:Type C Phospholipases