[The use of a dot immunoenzyme method for detection of viral antigens].
The technique of dot immunoenzyme detection has been elaborated for viral antigens identification. The investigated samples (extracts of infected cells, fractions obtained during viruses and viral proteins purification) are placed in nitrocellulose filters, the free binding sites are blocked and then treated with specific immune serum. The formed antigen-antibody complexes are detected using antispecies immunoglobulins or protein A from Staphylococcus aureus, conjugated with horseradish peroxidase. The brown dots appear at samples location containing the viral antigens. Sensitivity of the technique is 1 ng of protein per sample as tested using adenoviral antigens.
D000260:Adenoviruses, Human / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis*; D007124:Immunoenzyme Techniques
D000956:Antigens, Viral
Primenenie tochechnoĭ immunofermentnoĭ detektsii dlia vyiavleniia virusnykh antigenov.