Partial differentiation of rat egg cylinders in serum-free and protein-free medium.
Modified organ cultures of rat egg cylinders were grown for 2 weeks in Eagle's MEM without serum or with serum added at different times. Explant survival was decreased only in cultures grown for the entire 2 weeks in serum-free medium, whereas the explant growth was impeded in all but the cultures grown for 2 weeks in 50% MEM plus 50% serum. Differentiation of epidermis and cartilage in the cultures deprived of serum for the entire 2-week period was comparable to that in fully serum-supplemented medium, whereas other differentiated tissues were rare or absent. In explants cultivated without serum for only the first week, neuroblasts were scarce.
D000818:Animals; D001769:Blood; D002454:Cell Differentiation; D003470:Culture Media; D005260:Female; D009924:Organ Culture Techniques / Q000379:methods*; D010063:Ovum / Q000166:cytology*; D051381:Rats; D011916:Rats, Inbred F344
D003470:Culture Media