title: Electron microscopical structure analysis of yeast fatty-acid synthase at low resolution.
abstract: From an electron microscopical tilt series of the multi-enzyme complex yeast fatty-acid synthase eight three-dimensional molecular structures were obtained by three-dimensional reconstruction of single molecules. The structures confirm present concepts showing a well defined central wall and a sequential arrangement of protein domains in the form of "arches". Additional structural details as ring-shaped parts in the central walls are recognizable. Because of the flattening and the irregular structural deterioration of the single molecules three-dimensional averaging was only partially successful; however, a satisfactory average from five molecules could be obtained. Attempts to find the symmetry of the subunit arrangement by applying correlation methods and by establishing a novel type of correlation analysis ("correlation tables") did not yield a clear proof. However, several strong indications of D3 symmetry were found.
mesh_terms: D064429:Fatty Acid Synthases; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D064429:Fatty Acid Synthases