Expression of the bacteriophage T4 denV structural gene in Escherichia coli.
The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.
D003001:Cloning, Molecular; D004274:DNA, Recombinant; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D005796:Genes; D005814:Genes, Viral; D010957:Plasmids; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014466:Ultraviolet Rays
D004274:DNA, Recombinant; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer)