Susceptibility of human glial cells to infection with human immunodeficiency virus (HIV).
Three human brain-derived cell lines (including two of astrocytic origin) were exposed in vitro to the human immunodeficiency virus (HIV), the etiologic agent of immunodeficiency in AIDS. In all three lines, HIV transcripts were detected by in situ hybridisation in 20-30% of cells 48 h after infection. Synthesis of virus gag gene products p24 and p55 was demonstrated by immunoblotting. No cytopathic effects typical of HIV-infected human T lymphocytes were observed. Our data indicate that HIV is neurotropic, and support the hypothesis that this virus may infect astrocytes in the brain.
D000911:Antibodies, Monoclonal; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D006678:HIV / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D007163:Immunosorbent Techniques; D009457:Neuroglia / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D005904:Glial Fibrillary Acidic Protein; D004247:DNA