title: Determination of the nucleotide sequence for the exonuclease I structural gene (sbcB) of Escherichia coli K12.
abstract: The complete nucleotide sequence of the structural gene for Escherichia coli exonuclease I has been determined. The coding region corresponds to a 465-amino acid protein with molecular weight of 53,174. The partial amino acid sequence of purified exonuclease I agrees with that predicted by the DNA sequence. Two putative weak promoters have been localized by S1 nuclease analysis. The sbcB coding sequence contains many non-optimal codons, characteristic of many poorly expressed E. coli genes.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I