Interaction of T-2 toxin with bovine carrier erythrocytes: effects on cell lysis, permeability, and entrapment.
Hemolysis, morphological changes, binding, and effect on encapsulation of exogenous substances were used as a basis to study the interaction of the trichothecene mycotoxin, T-2 toxin, with erythrocytes. T-2 toxin did not cause hemolysis of bovine erythrocytes but readily hemolyzed rat erythrocytes. T-2 toxin interaction with bovine erythrocytes was minimal because T-2 toxin did not bind appreciably to the erythrocytes. Entrapment of T-2 toxin in carrier erythrocytes was independent of toxin concentration, and interaction of T-2 toxin with erythrocytes did not affect the entrapment of the markers sucrose or inulin. T-2 toxin rapidly diffuses from carrier erythrocytes with less than 20% remaining after 4 hr of incubation. Cross-linking of the erythrocyte membrane with glutaraldehyde prevents T-2 toxin efflux from carrier erythrocytes.
D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D051381:Rats; D012717:Sesquiterpenes / Q000633:toxicity*; D013045:Species Specificity; D013605:T-2 Toxin / Q000633:toxicity*
D012717:Sesquiterpenes; D013605:T-2 Toxin