Effect of insulin on glucose/oxygen and lactate/oxygen quotients across the hindlimb of fetal lambs.
In order to determine whether insulin stimulates glucose uptake by the hindlimb tissue of the fetal lamb, we performed 7 paired, euglycemic glucose 'clamp' experiments in 7 chronically prepared fetal sheep. Four sample sets for oxygen content, glucose and lactate concentration were drawn from the external iliac artery and vein during a control period and repeated during a euglycemic, hyperinsulinemic 'clamp' period. Insulin was infused at constant rate (4 mU/min/kg) and the fetal arterial glucose concentration was maintained by variable glucose infusion. The glucose/oxygen quotient increased significantly: 1.05 +/- 0.07 (control) versus 2.02 +/- 0.16 (hyperinsulinemia). There was no change in lactate/oxygen quotient. The data demonstrate that with maximal insulin stimulation, fetal hindlimb tissues increase glucose uptake by 92% with no large change in lactate production, indicating a net accumulation of glucose carbon under these experimental conditions.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D005312:Fetal Blood / Q000187:drug effects / Q000378:metabolism*; D006614:Hindlimb / Q000098:blood supply; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007773:Lactates / Q000097:blood*; D010100:Oxygen / Q000097:blood*; D012756:Sheep
D001786:Blood Glucose; D007328:Insulin; D007773:Lactates; D010100:Oxygen