Monoclonal antibodies to the two most basic papaya proteinases.
The proteinases from Carica papaya include papain, isoenzymes of chymopapain and two proteinases A and B distinguished by their unusually high pI. The identity of one of the most basic proteinases has been questioned. The present report describes the preparation and characterisation of two monoclonal antibodies that react specifically with papaya proteinases A and B respectively and a third that identifies a common structural feature found in papain and proteinase A.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002917:Chymopapain / Q000276:immunology; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000276:immunology*; D007163:Immunosorbent Techniques; D007526:Isoelectric Point; D008970:Molecular Weight; D010206:Papain / Q000276:immunology; D010940:Plant Proteins; D010944:Plants / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D010940:Plant Proteins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D010206:Papain; C050874:glycyl endopeptidase; C039974:caricain; D002917:Chymopapain