Quantitative microphotometric studies of Feulgen-stained nuclei on sections of the human uterine cervix: optimization of preparation and measuring technique.
The total extinction of apparently normal intermediate cell nuclei was microphotometrically measured in formalin-fixed Feulgen-stained paraffin sections of the uterine cervix. We investigated whether reproducible microphotometric results can be obtained from a histologic section expected to contain a certain amount of sectioned nuclei and stained by a standard technique. The results have shown that exact reproduction of microphotometrically measured mean total extinction values of intermediate cell nuclei can be achieved with a threshold value of 90% transmission in 10 microns sections.
D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D002584:Cervix Uteri / Q000378:metabolism / Q000648:ultrastructure*; D004396:Coloring Agents; D004247:DNA / Q000378:metabolism; D005260:Female; D006652:Histological Techniques; D006801:Humans; D008875:Middle Aged; D010783:Photometry / Q000379:methods*; D012394:Rosaniline Dyes
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA