title: Morphological and electrophysiological study of the inner ear and the central auditory pathways following whole body fetal irradiation.
abstract: Pregnant CBA/CBA mice were whole body irradiated with 2 Gy on the 13th or 16th day of gestation, respectively. The exposed fetuses were raised to an age of 21 postnatal days. Auditory brainstem recordings of threshold levels showed a considerable elevation independent of if irradiation had been performed on either the 13th gestational day or the 16th gestational day. In exposed animals a latency difference occurs in the peaks that increases from peak 1 to peak 5, measuring in peak 5 up to 1.16 ms. Also the peak-to-peak length of waves 1-5 increases in irradiated animals. Scanning electron microscopy of the cochleae showed varying degrees of stereociliary derangement of both outer and inner hair cells, particularly in cochleae where irradiation had been performed on the 13th gestational day, but not loss of hair cells. Light microscopic analysis of auditory brainstem nuclei revealed normal conditions except that in inner ears exposed on the 16th gestational day the flocculus was fused to the lateral surface of the anterior ventral cochlear nucleus. It is concluded that the elevated threshold levels in irradiated animals are most likely due to pathological changes in the peripheral receptor organ whereas the increased latencies and the increased peak-to-peak length likewise reflect functional changes in the brainstem auditory nuclei.
mesh_terms: D000818:Animals; D001306:Auditory Pathways / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects*; D001309:Auditory Threshold / Q000528:radiation effects; D001933:Brain Stem / Q000528:radiation effects; D002531:Cerebellum / Q000528:radiation effects; D003051:Cochlea / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects*; D004594:Electrophysiology; D005072:Evoked Potentials, Auditory / Q000528:radiation effects; D005260:Female; D005333:Fetus / Q000528:radiation effects*; D006198:Hair Cells, Auditory / Q000528:radiation effects; D006199:Hair Cells, Auditory, Inner / Q000528:radiation effects; D051379:Mice; D008808:Mice, Inbred CBA; D011247:Pregnancy; D013997:Time Factors; D014916:Whole-Body Irradiation