[Characteristic parameters of the population of circulating DNA in cases of disseminated lupus erythematosus ].
Important quantities of circulating DNA (up to congruent to 50 micrograms/ml) have been shown to be present in the sera of patients with serious systemic lupus erythematosus. This DNA is double-stranded, as demonstrated by colorimetric, enzymatic and biophysic tests. The circulating DNA population is made up of a main fraction at 250 base pairs and a heterogeneous tail up to 300 bases pairs, as demonstrated by electrophoretic measures on polyacrylamide gel. After hydrolysis in limited conditions with DNAse I and 32P labelling, the repartition of autoradiographed material confirms the reality of a main peak and of a more dispersed tail.
D001345:Autoradiography; D001482:Base Composition; D004247:DNA / Q000097:blood*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D010761:Phosphorus Radioisotopes
D010761:Phosphorus Radioisotopes; D004247:DNA; D003850:Deoxyribonuclease I
Paramètres caractéristiques de la population d'ADN circulant dans les cas de lupus erythémateux disséminé.