Immunohistological detection of Legionella pneumophila in lung sections.
Immunohistology was used for the detection of Legionella pneumophila serogroup I in necropsy tissue. Study of pneumonic lung from the recent Stafford outbreak has shown that this technique has a high sensitivity. A retrospective postmortem examination showed that L pneumophila serogroup 1 was an unusual cause of pneumonia in Oxfordshire during the study period. L pneumophila serogroup 1 can be successfully subgrouped, using a panel of monoclonal antibodies on formalin fixed paraffin embedded sections. Immunohistological methods have a potentially useful role in the diagnosis of Legionellosis at postmortem examination and in the epidemiological investigation of individual cases and outbreaks.
D000911:Antibodies, Monoclonal; D006801:Humans; D007124:Immunoenzyme Techniques; D007875:Legionella / Q000276:immunology / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000175:diagnosis* / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000382:microbiology*; D012189:Retrospective Studies; D012703:Serotyping
D000911:Antibodies, Monoclonal