Alterations of HLe-1 (T200) fluorescence intensity on acute lymphoblastic leukemia cells may relate to therapeutic outcome.
Quantitative differences in HLe-1 expression were studied on normal lymphocytes and lymphoblasts of patients with acute lymphoblastic leukemia (ALL). A relationship was found between quantitative antigen expression and therapeutic outcome. Alterations in fluorescence intensity (FI) were demonstrated using quantitative flow cytometric methods and the monoclonal antibody (MoAb) anti HLe-1 (T200). Lymphoblasts from patients with ALL produced FI peaks ranging form channel 17 to 112 (mean 68; n = 28), while normal lymphoid cells were at FI channel 127 +/- 3 (n = 121). Patients with the dimmest-staining lymphoblasts (channels 17-50) responded better to therapy than those with brighter-staining cells (channels 50-100). Data from this pilot study suggests that the FI of malignant lymphoblasts has implications in the clinical response to therapy.
D000293:Adolescent; D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005455:Fluorescent Antibody Technique; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000473:pathology*; D008214:Lymphocytes / Q000276:immunology*; D008875:Middle Aged; D011379:Prognosis
D000951:Antigens, Neoplasm; D000954:Antigens, Surface