Temperature sensitivity of the penicillin-induced autolysis mechanism in nongrowing cultures of Escherichia coli.
The effect of incubation temperature on the ampicillin-induced autolysis of nongrowing Escherichia coli was determined. The autolysis mechanisms in amino acid-deprived relA mutant cells treated with chloramphenicol were temperature sensitive. This temperature-sensitive autolysis was demonstrated in three independent ways: turbidimetric determinations, viable cell counts, and solubilization of radiolabeled peptidoglycan.
D000596:Amino Acids / Q000378:metabolism; D001329:Autolysis; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D010406:Penicillins / Q000494:pharmacology*; D010457:Peptidoglycan / Q000378:metabolism; D013696:Temperature
D000596:Amino Acids; D010406:Penicillins; D010457:Peptidoglycan; D002701:Chloramphenicol