L-asparaginase effects on inhibition of protein synthesis and lowering of the glutamine content in cultured rat hepatocytes.
Primary cultures of rat hepatocytes were exposed to various concentrations of L-asparaginase derived from Escherichia Coli. Protein synthesis was inhibited by about 33% and cellular glutamine was reduced proportionally to the enzyme concentration. However, protein synthesis was inhibited only by amounts of enzyme able to reduce glutamine to critical levels below 10 nmol/mg cell protein. These data suggest that the glutaminase activity which probably contaminates E. coli asparaginase may be responsible for reduced liver protein synthesis.
D000818:Animals; D001215:Asparaginase / Q000633:toxicity*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004926:Escherichia coli; D005973:Glutamine / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats
D005973:Glutamine; D003907:Dexamethasone; D001215:Asparaginase