Distribution of transferrin synthesis in brain and other tissues in the rat.
Levels of transferrin mRNA were measured by hybridization to transferrin cDNA in extracts from various areas of rat brain and other tissues. The highest concentrations of transferrin mRNA were found in the liver and the choroid plexus of the lateral and third ventricles. Lower concentrations were observed in the medulla and thalamus, choroid plexus of the fourth ventricle, cortex, hypothalamus, cerebellum, pituitary, testis, placenta, stomach, spleen, kidney, muscle, and heart. Yolk sac, small intestine, and adrenal glands did not contain detectable transferrin mRNA levels. The size of transferrin mRNA was the same in liver, brain, and testis. Upon incubation of choroid plexus pieces with [14C]leucine in vitro, about 4% of the radioactive protein secreted into the medium was found to be transferrin. Together with previous data (Dickson, P.W., Howlett, G.J., and Schreiber, G. (1985) J. Biol. Chem. 260, 8214-8219; Dickson, P.W., Aldred, A.R., Marley, P.D., Bannister, D., and Schreiber (1986) J. Biol. Chem. 261, 3475-3478) the obtained data suggest that the choroid plexus plays a role in maintenance of homeostasis in the microenvironment of the central nervous system by synthesizing and secreting plasma proteins.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002831:Choroid Plexus / Q000378:metabolism; D004247:DNA / Q000032:analysis; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011915:Rats, Inbred BUF; D014018:Tissue Distribution; D014168:Transferrin / Q000096:biosynthesis*
D012333:RNA, Messenger; D014168:Transferrin; D004247:DNA; D007930:Leucine