title: An altered adenylate cyclase in cdc35-1 cell division cycle mutant of yeast.
abstract: Adenylate cyclase activity was studied in Saccharomyces cerevisiae's cell division cycle (cdc) mutant 35-1. The temperature sensitive mutant cdc35-1 was previously mapped as an allele of cyr, the adenylate cyclase gene. However, the adenylate cyclase activities of membranes prepared from cdc35-1 were not thermosensitive. The adenylate cyclase activity of cdc35-1 was found to have an altered Mn2+ dependency and did not respond to Gpp(NH)p stimulation. These results suggest that cdc35-1 mutation may not be at the catalytic site but at a site where adenylate cyclase interacts with regulatory proteins.
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002384:Catalysis; D002453:Cell Cycle; D002455:Cell Division; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D000072235:Mating Factor; D009154:Mutation; D010455:Peptides / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D010455:Peptides; D006165:Guanylyl Imidodiphosphate; D008345:Manganese; D000072235:Mating Factor; D000262:Adenylyl Cyclases