Evaluation of immunosorbents for the analysis of small molecules. Isolation and purification of cytokinins.
This paper describes the preparation and evaluation of immunosorbents for the isolation of cytokinins. The nature of both the solid support and the linkage chemistry affected the non-specific adsorption of sample contaminants and the characteristics of the immobilized antibody. All the immunosorbents investigated provided sufficient purification of cytokinins for high-performance liquid chromatographic analysis with no further clean-up. This demonstrates a rapid and powerful purification method for small molecules for which antibodies can be generated. As a consequence, the procedures described for the immobilization of cytokinin antibodies are generally applicable to the preparation of immunosorbents.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003583:Cytokinins / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D010936:Plant Extracts / Q000032:analysis; D010937:Plant Growth Regulators / Q000302:isolation & purification*; D011817:Rabbits
D003583:Cytokinins; D007164:Immunosorbents; D010936:Plant Extracts; D010937:Plant Growth Regulators