title: Fixation of neural tissue for electron microscopy with an electronically controlled perfusion pump.
abstract: Many attempts to improve the perfusion of mammalian tissues aim at changes of the osmotic pressure. We describe a method for fixation of nervous tissues controlling both the hydrostatic pressure and the flow rate of a perfusion solution. The constancy of these parameters is guaranteed by an electronically controlled perfusion pump. Thus, a more uniform and complete preservation can be achieved. Further advantages of this method include provision for a rapid succession of rinsing and fixation solution and a continuous control of the hydrostatic pressure during perfusion.
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D006652:Histological Techniques; D008297:Male; D008854:Microscopy, Electron; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains