Reversed-phase high-performance liquid chromatographic separation of phenolic derivatives of benzo[a]pyrene, benz[a]anthracene, and chrysene with monomeric and polymeric C18 columns.
The separation of monohydroxylated derivatives (phenols) of benzo[a]pyrene, benz[a]anthracene, and chrysene was studied by reversed-phase high-performance liquid chromatography using a monomeric Zorbax ODS column and a polymeric Vydac C18 column. The Vydac C18 column resolved the phenols of each hydrocarbon with a wide range of retention times than the Zorbax ODS column. Four K-region phenols of benzo[a]pyrene are either not separated or marginally separated on both monomeric and polymeric columns. Other K-region and non-K-region phenols of all three hydrocarbons can be separated by using the monomeric and polymeric columns in combination.
D000818:Animals; D001551:Benz(a)Anthracenes / Q000302:isolation & purification*; D001564:Benzo(a)pyrene / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002911:Chrysenes / Q000302:isolation & purification*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010616:Phenanthrenes / Q000302:isolation & purification*; D010634:Phenobarbital / Q000494:pharmacology; D010636:Phenols / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains
D001551:Benz(a)Anthracenes; D002911:Chrysenes; D010616:Phenanthrenes; D010636:Phenols; D001564:Benzo(a)pyrene; D010634:Phenobarbital