The nucleotide sequence of the pepN gene and its over-expression in Escherichia coli.
The complete nucleotide sequence has been determined for the pepN gene of Escherichia coli K-12. The product of this gene, peptidase N, is apparently 870 amino acids in length. The coding sequence is followed by a tandem pair of stop codons and then a sequence capable of forming a stem-and-loop structure in the pepN mRNA. In the process of subcloning the pepN gene we constructed a plasmid which causes peptidase N to be produced at a level of 50% of total protein. The peptidase is fully active and completely soluble and these overproducing cells appear otherwise normal.
D000626:Aminopeptidases / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D010957:Plasmids; D011401:Promoter Regions, Genetic
D003062:Codon; D004269:DNA, Bacterial; D000626:Aminopeptidases; C034113:peptidase N