Expression of ricin A chain in Escherichia coli.
DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.
D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012276:Ricin / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology
D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D004247:DNA; D012276:Ricin