Structure and activity of the translocated c-myc in mouse plasmacytoma XRPC-24.
In the mouse plasmacytoma XRPC-24 both c-mos and c-myc are rearranged. We cloned the rearranged c-myc and found that it was translocated to the immunoglobulin C alpha locus. The breakpoint is at the end of exon 1 in c-myc and approximately 0.5 kb upstream from exon 1 of C alpha. The cloned translocated c-myc linked to a strong transcriptional promoter can efficiently transform rat embryo fibroblasts when co-transfected with the activated Ha-ras. The transformed cells are tumorigenic in syngeneic rats.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis; D005347:Fibroblasts; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D010954:Plasmacytoma / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D051381:Rats; D014162:Transfection; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D004274:DNA, Recombinant; D007127:Immunoglobulin Constant Regions; D007136:Immunoglobulins; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc