Different specificities of cloned T cells assessed by in vitro proliferation assays and by the ability to mediate skin graft rejection in vivo.
The experiments presented here have compared the specificities of T cell clones as determined by in vitro proliferative responses and their specificities as reflected by their ability to mediate skin graft rejection in vivo. Two proliferative T cell clones with distinct in vitro specificities were evaluated for their ability to mediate rejection of skin grafts from C57BL/10 Scn nu/nu mice. Clone 14.11 (L3T4+, Lyt2-) was specifically stimulated to proliferate in vitro by I-Ad determinants, while clone 3.3.10 (also L3T4+, Lyt2-) recognized M1sa products in the context of MHC-encoded cell surface determinants. The results demonstrate that both clone 14.11 and clone 3.3.10 T cells are capable of mediating rejection of DBA/2 (H-2d, M1sa) skin grafts from B10 nu/nu mice. Surprisingly, neither clone 3.3.10 T cells nor clone 14.11 T cells were found to be effective at rejecting skin grafts from the D1.C congenic donor strain of mice (also H-2d, M1sa) from B10 nu/nu mice. Further, clone 14.11 T cells were also found to be ineffective at rejecting B10.D2 (H-2d, M1sb) skin grafts from B10 nu/nu mice. These data indicate the existence of a striking dichotomy between the specificity of alloreactive T cell clones as determined by in vitro proliferation studies and their functional capabilities in vivo.
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D002455:Cell Division; D002999:Clone Cells / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006084:Graft Rejection; D006183:H-2 Antigens / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008811:Mice, Inbred DBA / Q000276:immunology; D008815:Mice, Inbred Strains / Q000276:immunology; D008819:Mice, Nude / Q000276:immunology; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D006183:H-2 Antigens