Biosynthetic preparation of radioactively labelled ethanolamine plasmalogen (1-O-[1'-14C]octadec-1'-enyl-2-acyl-sn-glycero-3-phosphoethanola mine) using a protozoan cell culture.
Ethanolamine plasmalogen radiolabelled mainly in the O-alkenyl moiety was prepared from cell suspension cultures of the flagellate Leishmania donovani previously incubated with [1-14C]octadecanol over one growth period. The optimal concentration of [1-14C]octadecanol for labelling was shown to be 1 microM, when 60% of total lipid radioactivity appeared in the 1,2-diradyl-sn-glycero-3-phosphoethanolamine fraction, with an overall yield of approx. 35%. Analysis of this fraction revealed that 93% of the label was present in O-octa-dec-1-enyl, 3% in O-alkyl and 4% in acyl moieties. A specific radioactivity of approx. 14 mCi/mmol was determined. Raising the culture medium concentration of [1-14C]octadecanol to 2 microM yielded a product with a specific radioactivity of 25 mCi/mmol.
D000818:Animals; D002250:Carbon Radioisotopes; D005233:Fatty Alcohols / Q000378:metabolism; D007700:Kinetics; D007893:Leishmania donovani / Q000254:growth & development / Q000378:metabolism*; D010955:Plasmalogens / Q000096:biosynthesis* / Q000302:isolation & purification; D011865:Radioisotope Dilution Technique
D002250:Carbon Radioisotopes; D005233:Fatty Alcohols; D010955:Plasmalogens; C020791:phosphatidal ethanolamines; C009316:stearyl alcohol