title: Cytochemical detection of carbohydrate and immunocytochemical detection of relaxin in the same secretory granule.
abstract: We administered estradiol and progesterone to spayed guinea pigs, with resultant accumulation of secretory granules in endometrial gland cells. By initially employing protein A-colloidal gold immunolocalization of relaxin, followed by cytochemical staining of carbohydrate with the thiocarbohydrazide-silver proteinate method on the same section, we showed clearly that the secretory granules were composed of a central core containing relaxin and a cortex of carbohydrate-rich material. Use of normal rabbit serum rather than relaxin antiserum, and omission of periodic acid, demonstrated the specificity of the technique.
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004717:Endometrium / Q000166:cytology / Q000648:ultrastructure*; D004958:Estradiol / Q000008:administration & dosage; D005260:Female; D006168:Guinea Pigs; D006651:Histocytochemistry; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D011374:Progesterone / Q000008:administration & dosage; D012065:Relaxin / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D011374:Progesterone; D004958:Estradiol; D012065:Relaxin