Inhibition of taurocholate efflux from rat hepatic canalicular membrane vesicles by glutathione disulfide.
In right-side out rat hepatic canalicular membrane vesicles glutathione disulfide (GSSG) inhibited the efflux of taurocholate approx. 70% in the presence or approx. 55% in the absence of a valinomycin-mediated K+ diffusion potential; maximal inhibition occurred at 5 mM GSSG. The inhibition by GSSG was abolished by dithioerythritol. Neither dithioerythritol alone nor GSH inhibited taurocholate efflux. S-(2,4-Dinitrophenyl)glutathione and N-ethylmaleimide showed intermediate inhibitory effects.
D000818:Animals; D001648:Bile Canaliculi / Q000187:drug effects / Q000378:metabolism*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism*; D001693:Biological Transport, Active; D004226:Dithioerythritol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005033:Ethylmaleimide / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D019803:Glutathione Disulfide; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013656:Taurocholic Acid / Q000378:metabolism*; D014634:Valinomycin / Q000494:pharmacology
D014634:Valinomycin; D013656:Taurocholic Acid; D004226:Dithioerythritol; D005978:Glutathione; D005033:Ethylmaleimide; D019803:Glutathione Disulfide