title: Identification of 17 alpha,20 beta,21-trihydroxy-4-pregnen-3-one as the major ovarian steroid produced by the teleost Micropogonias undulatus during final oocyte maturation.
abstract: This study describes the identification of 17 alpha,20 beta, 21-trihydroxy-4-pregnen-3-one (20 beta-dihydro-11-deoxycortisol, 20 beta-S) as a major steroid product of the ovary of Atlantic croaker (Micropogonias undulatus) incubated in vitro. This is the first report which describes 17 alpha,20 beta,21-trihydroxy-4-pregnen-3-one as a major product of teleost steroidogenic tissue. The steroid was identified by a variety of methods, including HPLC, TLC, GC-MS, UV absorbance, and reactions with specific enzymes. Full grown oocytes, prior to final oocyte maturation (FOM), accumulated only small amounts of 20 beta-S. However, a substantial increase in 20 beta-S production occurred at the time of FOM. These results suggest that 20 beta-S is a maturational steroid in this species.
mesh_terms: D015065:17-Hydroxycorticosteroids; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003350:Cortodoxone / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D005399:Fishes; D008401:Gas Chromatography-Mass Spectrometry; D009865:Oocytes / Q000032:analysis / Q000254:growth & development*; D011284:Pregnenolone / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015065:17-Hydroxycorticosteroids; C051954:17,20,21-trihydroxy-4-pregnen-3-one; D011284:Pregnenolone; D003350:Cortodoxone