Extraction of cellular DNA from human cells and tissues fixed in ethanol.
DNA can be extracted from ethanol-fixed lymphoid cells and tissues. The fixation procedure is simple and rapid, and the DNA extraction itself is the same as that normally used for fresh tissue or cells. DNA extracted from ethanol-fixed material is indistinguishable from DNA extracted from fresh samples based on its purity, its ability to be digested with restriction endonucleases, and its ability to specifically hybridize to DNA probes. The capability to extract DNA from ethanol-fixed cells and tissues eliminates the need for stringent handling and storage requirements of fresh or frozen specimens.
D004247:DNA / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D000431:Ethanol; D006652:Histological Techniques; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000166:cytology; D008223:Lymphoma / Q000032:analysis* / Q000473:pathology
D004273:DNA, Neoplasm; D000431:Ethanol; D004247:DNA