Antimicrobial binding of a radiolabeled cationic neutrophil granule protein.
A 57,000-dalton protein (CAP57) purified from human polymorphonuclear leukocytes has antimicrobial activity against a number of gram-negative bacteria. We developed a procedure using solid-phase Iodo-gen to radiolabel CAP57 without destroying its antibacterial activity. Iodinated and native CAP57 were electrophoretically identical. Autoradiographs of sodium dodecyl sulfate-polyacrylamide gels revealed greater than 95% of the 125I in a single heavy band in the 57,000-molecular-weight region. The quantity of [125I]CAP57 bound to bacterial test strains was directly proportional to the sensitivity to CAP57.
D023181:Antimicrobial Cationic Peptides; D001419:Bacteria / Q000378:metabolism*; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009504:Neutrophils / Q000502:physiology; D012486:Salmonella typhimurium / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; C030509:neutrophil basic proteins