The calibration of rabbit tissue thromboplastins: experience of the Dutch Reference Laboratory for anticoagulant control.
A number of commercial rabbit tissue thromboplastins used in oral anticoagulant control have been calibrated against the first International Reference Preparation for thromboplastins. This was done in a three-stage procedure by one laboratory, each stage representing a different level of thromboplastin comparability. The calibration model recently recommended by ICTH and ICSH was tested. This model proved to be suitable, although a statistically significant aberration was observed for some of the thromboplastins. The bias introduced by using the model in these non-ideal cases was small compared to the overall variation of the International Normalized Ratio, being the universal scale for reporting the prothrombin time during oral anticoagulant control. Batch-to-batch calibration using lyophilized pooled plasmas could be reliably performed for several commercial thromboplastins.
D000284:Administration, Oral; D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage / Q000592:standards*; D001777:Blood Coagulation / Q000187:drug effects; D006801:Humans; D007394:International System of Units / Q000592:standards; D011517:Prothrombin Time; D011817:Rabbits; D012015:Reference Standards; D013223:Statistics as Topic; D013925:Thromboplastin / Q000008:administration & dosage / Q000592:standards*
D000925:Anticoagulants; D013925:Thromboplastin