[Identification of insect cell lines].
Identification of 10 cell lines of 6 insect species received by the cell culture collection of Molecular Biology Institute from different research institutions of the USSR was carried out by karyological analysis and determinations of the spectrum of isoforms of the following enzymes: glucose-6-phosphate dehydrogenase (G-6-PDH), lactate dehydrogenase (LDH), isocitrate dehydrogenase (ICDH), and superoxide dismutase (SOD). Nine out of the 10 cell lines examined were shown to be identical to those of gypsy moth. Possible ways of cell contamination with other cells in long-term cultivation and preparation of new lines were determined.
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding / Q000379:methods; D003584:Cytological Techniques; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D007313:Insecta / Q000166:cytology*; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008677:Metaphase; D013482:Superoxide Dismutase / Q000032:analysis; D014776:Virus Cultivation
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013482:Superoxide Dismutase
Identifikatsiia liniĭ kletok nasekomykh.