title: Initial observations of a kallikrein-like enzyme associated with the plasma membranes of rat adipocytes.
abstract: Homogenates of rat adipocytes and plasma membranes thereof were shown by radioimmunoassay to contain immunoreactive glandular kallikrein. On the basis of the hydrolysis of D-Val-Leu-Arg-p-nitroanilide, the kallikrein-like enzyme associated with the plasma membranes was found not to be stimulated by prior incubation with melittin or phospholipase A2. However, pre-incubation of the membrane preparation with trypsin did increase the activity of the enzyme. Furthermore, activation could also be achieved by incubating the plasma membranes with insulin at a dose that stimulated glucose uptake into intact adipocytes. On the other hand, incubation with insulin at a dose that did not increase glucose uptake into rat adipocytes was ineffective in activating the kallikrein-like enzyme.
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003429:Cross Reactions; D007106:Immune Sera; D007328:Insulin / Q000494:pharmacology; D007610:Kallikreins / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D010741:Phospholipases A / Q000494:pharmacology; D054467:Phospholipases A2; D011863:Radioimmunoassay; D051381:Rats; D013363:Submandibular Gland / Q000201:enzymology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D007106:Immune Sera; D007328:Insulin; D010741:Phospholipases A; D054467:Phospholipases A2; D007610:Kallikreins; D014357:Trypsin