The use of 3,3',5,5'-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay.
The assay conditions for the use of 3,3',5,5' tetramethylbenzidine (TMB) in microplate enzyme-linked immunosorbent assay are described. TMB is a safe (non-mutagenic) chromogen that is more sensitive than OPD as a substrate for horseradish peroxidase. We describe the optimum storage and assay conditions for this chromogen.
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D001560:Benzidines; D002863:Chromogenic Compounds; D004797:Enzyme-Linked Immunosorbent Assay; D005872:Giardia / Q000276:immunology; D006735:Horseradish Peroxidase; D013379:Substrate Specificity
D000953:Antigens, Protozoan; D001560:Benzidines; D002863:Chromogenic Compounds; C021758:3,3',5,5'-tetramethylbenzidine; D006735:Horseradish Peroxidase