Labeling of sarcoma associated monoclonal antibody with 111In, 67Ga and 125I.
Labeling of human sarcoma-associated murine monoclonal antibody (MAb) 23H7 with 67Ga and 111In by the bifunctional ligand method is reported. 67Ga was chelated to the MAb via desferrioxamine B and 111In via the cyclic anhydride of DTPA. Higher specific activity was obtained with 67Ga (4-5 microCi/micrograms) as compared with 111In (2 microCi/micrograms). The binding capacity of the MAb was confirmed by repeated indirect immuno-fluorescence assays performed before and after labeling. A fast blood clearance was observed: 33% recovered dose (R.D.) blood level 3 h post-injection as compared with 56% after injection of control polyclonal IgG. Preliminary results on chemically induced sarcoma bearing mice showed a relatively high tumor uptake of the labeled antibody.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000912:Antibodies, Neoplasm; D005710:Gallium Radioisotopes; D006801:Humans; D007204:Indium; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D051379:Mice; D011868:Radioisotopes; D012509:Sarcoma / Q000276:immunology*; D012513:Sarcoma, Experimental / Q000378:metabolism; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D005710:Gallium Radioisotopes; D007457:Iodine Radioisotopes; D011868:Radioisotopes; D007204:Indium