Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides.
The "polymerase chain reaction" (PCR) procedure for amplifying specific gene sequences has recently been combined with sequence-specific oligonucleotide (SSO) probe hybridization to develop a highly sensitive, rapid, and simple method for analyzing allelic variations in genomic DNA. In the present study we have used PCR/SSO to analyze partially purified DNA extracted from formalin-fixed, paraffin-embedded tissue specimens. We report that this DNA, including samples that were partially degraded, proved to be suitable for analysis by the PCR/SSO procedure.
D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D005404:Fixatives; D005557:Formaldehyde; D005838:Genotype; D005914:Globins / Q000235:genetics*; D006652:Histological Techniques; D006801:Humans; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D010232:Paraffin
D005404:Fixatives; D009841:Oligonucleotides; D005557:Formaldehyde; D010232:Paraffin; D005914:Globins; D004247:DNA; D004259:DNA-Directed DNA Polymerase