Mass spectral analysis of complex lipids desorbed directly from lyophilized membranes and cells.
Three desorption ionization techniques--laser desorption, plasma desorption and fast atom bombardment mass spectrometry--have been applied to lyophilized cells, membranes, lysed cells and various extracts. It has been shown that intact polar lipids are selectively desorbed from biological membranes by these methods and that their mass spectra provide "fingerprints" which reflect the unique biochemical composition of each class of cell or membrane.
D000818:Animals; D001923:Brain Chemistry; D002554:Cerebrosides / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis*; D005612:Freeze Drying; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry; D008563:Membrane Lipids / Q000032:analysis; D009186:Myelin Sheath / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D013433:Sulfoglycosphingolipids / Q000032:analysis
D002554:Cerebrosides; D008055:Lipids; D008563:Membrane Lipids; D010743:Phospholipids; D013433:Sulfoglycosphingolipids