title: [Mutants of Escherichia coli K-12 with increased resistance to ionizing radiation. VI. Increased radioresistance and heat shock proteins].
abstract: By means of one-dimensional electrophoresis, it is shown that in radiation-resistant Gamr444 and Gamr445 mutants of Escherichia coli K-12 high-molecular weight heat shock proteins are hyperproduced at 32-37 degrees C and are induced more intensively during heat shock (in comparison to the parental wild-type strain AB1157). When the missense htpR15 mutation of the positive regulatory htpR gene for heat shock proteins was introduced by transduction into the genome of the Gamr444 mutant, its enhanced radiation-resistance disappeared but could be restored upon introduction of pKV3 plasmid bearing the htpR+ gene. These data show that heat shock proteins are participating in the enhanced radioresistance of Gamr mutants.
mesh_terms: D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D011836:Radiation Tolerance; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D006360:Heat-Shock Proteins; D011938:Rec A Recombinases
vernacular_title: Mutanty Escherichia coli K-12 s povyshennoĭ ustoĭchivost'iu k ioniziruiushcheĭ radiatsii. Soobshchenie VI. Povyshennaia radiorezistentnost' i belki teplovogo shoka.