title: Expression of the bacteriophage T4 denV structural gene in Escherichia coli.
abstract: The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.
mesh_terms: D003001:Cloning, Molecular; D004274:DNA, Recombinant; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D005796:Genes; D005814:Genes, Viral; D010957:Plasmids; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: D004274:DNA, Recombinant; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer)