A practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity.
A known ornithine decarboxylase assay working with ion exchange separation of [3H]ornithine and [3H]putrescine has been revised. The assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. The separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. The detection limit is about 50 pmoles [3H]putrescine formed.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002852:Chromatography, Ion Exchange / Q000379:methods; D007700:Kinetics; D009952:Ornithine / Q000302:isolation & purification; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011700:Putrescine / Q000302:isolation & purification; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D014316:Tritium; D011732:Pyridoxal Phosphate; D009952:Ornithine; D009955:Ornithine Decarboxylase; D011700:Putrescine