Enzymatic assays for 2-deoxyglucose and 2-deoxyglucose 6-phosphate.
Methods for 2-deoxyglucose (2-DG) and 2-deoxyglucose 6-phosphate (DG6P) are described which are based on the fact that DG6P is oxidized by glucose-6-phosphate dehydrogenase (G6PDH), but at a rate 1000-fold slower than for glucose 6-phosphate, whereas hexokinase phosphorylates 2DG and glucose at comparable rates. Therefore, by adding the two enzymes in a suitable order, and in appropriate concentrations, 2DG, glucose, DG6P, and glucose 6-P can all be separately measured. To avoid a side reaction from the use of a high level of G6PDH, when measuring DG6P, glucose is first removed with glucose oxidase plus aldose reductase.
D001923:Brain Chemistry; D003837:Deoxy Sugars / Q000032:analysis*; D003847:Deoxyglucose / Q000032:analysis*; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005958:Glucosephosphates / Q000032:analysis*; D006593:Hexokinase / Q000032:analysis; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D003837:Deoxy Sugars; D005958:Glucosephosphates; C015785:2-deoxyglucose-6-phosphate; D019298:Glucose-6-Phosphate; D003847:Deoxyglucose; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase