[Optimization of conditions for preparation of the solid phase of infected cells in the immunoenzyme analysis of monoclonal antibodies].
The conditions of immunoenzyme assay have been studied on the solid state phase of infected cells using the model of monoclonal antibodies MAK-14-7 to the virus of Venezuelan equine encephalomyelitis (VVEE) and monoclonal antibodies OKA-1 to vaccine virus in the systems of VNK-21 cells or 4647 cells infected by VVEE, or HeLa cells infected by vaccine virus. The titer of monoclonal antibodies detected grows with the dose of infected cells fixed in the holes of micropanel used for reaction and with the multiplicity of infection. The most intensive and contrasting dyeing of conjugate has been registered when the cells have been fixed with 0.25% glutaraldehyde 24 h after infection. The titers of ascytic preparations of monoclonal antibodies MAK-14-7 and OKA-1 under the optimal conditions of immunoenzyme assay reaction on the solid phase of infected cells present 1 : 10 000 and 1 : 100 000.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002478:Cells, Cultured; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*
D000911:Antibodies, Monoclonal
Optimizatsiia usloviĭ prigotovleniia tverdoĭ fazy infitsirovannykh kletok dlia immunofermentnogo analiza monoklonal'nykh antitel.