title: Evidence for the existence of an expressed minor variant tRNAPhe in yeast.
abstract: Two expressed brewer's yeast tRNAsPhe, a major and a minor one, have been purified and sequenced. The major tRNAPhe corresponds to the already known tRNAPhe, whereas the minor one differs from the former in the substitution of T6-A67 by C6-G67 base pair in the "acceptor stem". The minor tRNAPhe contaminates all preparations of yeast tRNAPhe except those prepared by polyacrylamide gel electrophoresis.
mesh_terms: D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D009690:Nucleic Acid Conformation; D010649:Phenylalanine; D012343:RNA, Transfer / Q000032:analysis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000235:genetics
chemical_list: D010649:Phenylalanine; D012343:RNA, Transfer