title: Spot-transformation with plasmids.
abstract: A method is described which allows transformation of bacterial cells on the surface of agar plates. It is suitable for transferring large numbers of different plasmids, such as gene libraries, into a new genetic background. One nanogram of plasmid DNA is sufficient for transformation.
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D008722:Methods; D010957:Plasmids; D014169:Transformation, Bacterial