An approach to DNA fragment synthesis from unprotected nucleoside.
Oligodeoxynucleotides have been synthesized from unprotected nucleoside by use of morpholinophosphordichloridite as a phosphorylating reagent. This procedure consists of three (in situ) steps; the reaction of 5'-O-protected deoxynucleotide with morpholinophosphordichloridite, the reaction of the resulting on active mononucleotide derivative with the second nucleoside, non-aqueous oxidation, and then removal of amino moiety at phosphate diester linkage.
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D003853:Deoxyribonucleosides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
D003853:Deoxyribonucleosides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA