Y-body detection in formalin-fixed lymph nodes, using proteolytic enzymes and oxidative deamination.
The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine-T and Sodiumhypochlorite) on formalin-fixed paraffin-embedded lymph nodes with regard to detection of fluorescent Y-bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y-body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.
D002700:Chloramines; D005453:Fluorescence; D005557:Formaldehyde; D006651:Histocytochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008198:Lymph Nodes / Q000166:cytology*; D008297:Male; D008722:Methods; D010232:Paraffin; D010447:Peptide Hydrolases / Q000378:metabolism*; D012973:Sodium Hypochlorite; D014105:Tosyl Compounds; D014998:Y Chromosome / Q000032:analysis*
D002700:Chloramines; D007202:Indicators and Reagents; D014105:Tosyl Compounds; C016300:chloramine-T; D005557:Formaldehyde; D010232:Paraffin; D012973:Sodium Hypochlorite; D010447:Peptide Hydrolases