Trace amounts of ganglioside GM1 in human milk inhibit enterotoxins from Vibrio cholerae and Escherichia coli.
Gangliosides were isolated from human milk fat and purified by silica gel column chromatography and high performance liquid chromatography (HPLC). Low amounts of the ganglioside GM1, detected by high performance thin layer chromatography (HPTLC)-immunoassay, were found in all fractions with enterotoxin-inhibitory activity, while fractions without GM1 were inactive. It is concluded that GM1 is responsible for enterotoxin-inhibitory activity in the ganglioside fraction from human milk.
D002855:Chromatography, Thin Layer; D004768:Enterotoxins / Q000037:antagonists & inhibitors*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D005260:Female; D005677:G(M1) Ganglioside / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008895:Milk, Human / Q000032:analysis / Q000502:physiology; D011863:Radioimmunoassay; D014734:Vibrio cholerae
D004768:Enterotoxins; D005677:G(M1) Ganglioside