Rapid detection of group B streptococci directly from vaginal swabs.
Duplicate vaginal swabs were obtained from patients who attended obstetric or gynecologic clinics affiliated with the Magee Womens Hospital in Pittsburgh. One swab was cultured semiquantitatively on 5% sheep blood agar to detect group B streptococci (GBS). The other swab was subjected to a rapid method (25 min) for antigen detection and micronitrous acid exposure to extract the GBS antigen, followed by latex particle agglutination. A total of 464 swabs were evaluated by direct plating. Fifty-two swabs (11.2%) were found to contain GBS. Overall, the rapid method detected 21 of 52, or 40.4%, positive specimens. The sensitivity of the rapid method for identifying the most heavily colonized samples was 85.7%. This method can be used to identify maternity patients who are heavily colonized with GBS and are at high risk of delivering septic infants.
D000942:Antigens, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D007841:Latex Fixation Tests; D011247:Pregnancy; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology*
D000942:Antigens, Bacterial