Retrospective confirmation of Mycoplasma infection by the immunoperoxidase technique.
Rat tissues embedded in paraffin blocks stored for 8-10 yr were sectioned for immunoperoxidase staining. Sections of lung, trachea and endometrium of 22 of 32 rats that had exhibited clinical symptoms of mycoplasmal pneumonia prior to euthanasia, and whose organs had shown gross lesions suggesting mycoplasmal infection on necropsy, demonstrated the presence of Mycoplasma pulmonis organisms by the immunoperoxidase technique in one or more of these tissues.
D000818:Animals; D005260:Female; D005831:Genital Diseases, Female / Q000175:diagnosis / Q000382:microbiology; D007124:Immunoenzyme Techniques; D008297:Male; D009174:Mycoplasma / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D051381:Rats; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000382:microbiology