Demonstration of an epidermal growth factor-dependent 58 kDa phosphoprotein secreted by rat kidney fibroblasts.
Epidermal growth factor and 12-O-tetradecanoylphorbol-13-acetate increased the amount of 32Pi found as phosphoserine in a major, hitherto not described 58 kDa phosphoprotein (pp58) secreted by normal rat kidney fibroblasts. Platelet-derived growth factor, insulin, nerve growth factor and fibroblast growth factor did not affect pp58 while transforming growth factor beta decreased the accumulation of radioactivity into pp58. Cycloheximide, actinomycin D and ammonium chloride suppressed the labelling of pp58.
D000818:Animals; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D007668:Kidney / Q000166:cytology*; D051379:Mice; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015291:Transforming Growth Factors
D007328:Insulin; D009414:Nerve Growth Factors; D010455:Peptides; D010750:Phosphoproteins; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D013755:Tetradecanoylphorbol Acetate