Grafting of cultured allogeneic epidermis on second- and third-degree burn wounds on 26 patients.
Twenty-six individuals with second- and third-degree burn wounds have been grafted with cultured allogeneic epidermal cells. These epidermal cell grafts were grown in culture from cadaver skin according to a technique which we have developed. After being grafted with cultured allogeneic epidermal cells, superficial wounds, e.g., donor sites, healed within 7 days, compared to 14 days for mirror image control sites. Deep second-degree burn wounds which were excised before grafting with cultured cells healed in a mean time of 10 days. Deep second-degree burn wounds which were not excised before grafting healed in a mean time of 14 days. The cultured cells produced rapid healing in 11 of the 12 patients with deep second-degree burn wounds. The deep second-degree wounds grafted with cultured allogeneic epidermal cells healed with results which were comparable to the deep second-degree wounds which were autografted. Grafts of cultured allogeneic epidermal cells placed on full-thickness, or third-degree burn, wounds did not grow well.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002056:Burns / Q000145:classification / Q000601:surgery*; D002478:Cells, Cultured; D002648:Child; D000078404:Epidermal Cells; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016038:Skin Transplantation; D014945:Wound Healing