A shuttle vector system for Brevibacterium lactofermentum.
We have constructed a shuttle vector that replicates in Escherichia coli, Corynebacterium glutamicum and Brevibacterium lactofermentum, by fusion of a 4.4-kb cryptic plasmid isolated from B. lactofermentum and a derivative of pBR322. Resistance to erythromycin which is expressed in all three bacteria has been a useful selective marker. The frequency of homospecific transformation was 1.5 X 10(5) transformants/micrograms of hybrid plasmid DNA.
D001951:Brevibacterium / Q000187:drug effects / Q000235:genetics*; D003352:Corynebacterium / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D010957:Plasmids; D011815:R Factors; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004917:Erythromycin