[Effect of the irradiation conditions on the radiation inactivation of subtilisin-72].
A comparative study was made of gamma-inactivation of subtilisin-72 solutions in 5 X 10(-3) M acetate buffer and 0.1 M NaCl in the presence and absence of Ca2+ ions. It was shown that the acetate buffer had a protective action, and the influence of Ca2+ ions depended on the ionic strength of the solution. In general, Ca2+ ions exerted a stabilizing effect irrespective of the subtilisin concentration in the acetate buffer, but this effect competed with the destabilizing influence of the ionic strength increased by Ca2+ ions.
D000085:Acetates / Q000494:pharmacology; D002021:Buffers; D002118:Calcium / Q000494:pharmacology; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D011854:Radiochemistry; D012965:Sodium Chloride / Q000494:pharmacology; D012996:Solutions; D013381:Subtilisins / Q000037:antagonists & inhibitors / Q000528:radiation effects*
D000085:Acetates; D002021:Buffers; D012996:Solutions; D012965:Sodium Chloride; D013381:Subtilisins; D002118:Calcium
Vliianie usloviĭ oblucheniia na radiatsionnuiu inaktivatsiiu subtilizina-72.