title: Evaluation of immunosorbents for the analysis of small molecules. Isolation and purification of cytokinins.
abstract: This paper describes the preparation and evaluation of immunosorbents for the isolation of cytokinins. The nature of both the solid support and the linkage chemistry affected the non-specific adsorption of sample contaminants and the characteristics of the immobilized antibody. All the immunosorbents investigated provided sufficient purification of cytokinins for high-performance liquid chromatographic analysis with no further clean-up. This demonstrates a rapid and powerful purification method for small molecules for which antibodies can be generated. As a consequence, the procedures described for the immobilization of cytokinin antibodies are generally applicable to the preparation of immunosorbents.
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003583:Cytokinins / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D010936:Plant Extracts / Q000032:analysis; D010937:Plant Growth Regulators / Q000302:isolation & purification*; D011817:Rabbits
chemical_list: D003583:Cytokinins; D007164:Immunosorbents; D010936:Plant Extracts; D010937:Plant Growth Regulators