title: GABAergic input of cholecystokinin-immunoreactive neurons in the hilar region of the rat hippocampus. An electron microscopic double immunostaining study.
abstract: Double immunostaining was performed for electron microscopy to analyze the synaptic connections between glutamate decarboxylase (GAD)-immunoreactive axons and cholecystokinin (CCK)-immunoreactive neurons in the hilar region of the rat hippocampal formation. Following immunostaining for CCK, the diaminobenzidine (DAB) reaction product was silver-intensified and gold-substituted. In a subsequent second immunostaining for GAD, the immunoreactive elements were labeled using a single DAB reaction. Electron microscopic analysis of the double-stained Vibratome sections demonstrated that the single DAB-labeled GAD-immunoreactive boutons form symmetrical synaptic connections on the soma and primary dendrites of the DAB-gold-labeled CCK-immunoreactive neurons.
mesh_terms: D015100:3,3'-Diaminobenzidine; D000818:Animals; D001369:Axons / Q000201:enzymology; D002766:Cholecystokinin / Q000032:analysis*; D005260:Female; D005968:Glutamate Decarboxylase / Q000032:analysis*; D006624:Hippocampus / Q000032:analysis / Q000648:ultrastructure*; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009474:Neurons / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000032:analysis*
chemical_list: D015100:3,3'-Diaminobenzidine; D002766:Cholecystokinin; D005968:Glutamate Decarboxylase