Regulation of hexose transport in cultured bovine retinal microvessel endothelium by insulin.
In microvessel endothelium obtained from young calf retinas, insulin stimulated the uptake of 2-deoxy-D-glucose in a dose-dependent manner. The maximal level of glucose uptake (50-100% over basal values) was observed at 1 ng ml-1 insulin. The effects of insulin on glucose uptake were time-dependent, with optimum stimulation of glucose transport observed after 90 min of insulin treatment. The effects of insulin were prevented by 15 min pretreatment with 2 micrograms ml-1 cycloheximide or by 2 micrograms ml-1 actinomycin D, suggesting a role for new protein synthesis in the actions of insulin on glucose transport in this cell type. These results demonstrate that cultured retinal microvessel endothelial cells exhibit an insulin-sensitive glucose transport system.
D019325:3-O-Methylglucose; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism / Q000031:analogs & derivatives; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D008757:Methylglucosides / Q000378:metabolism; D012171:Retinal Vessels / Q000187:drug effects / Q000378:metabolism*; D013268:Stimulation, Chemical; D013997:Time Factors
D007328:Insulin; D008757:Methylglucosides; D019325:3-O-Methylglucose; D003609:Dactinomycin; D003513:Cycloheximide; D003847:Deoxyglucose; D005947:Glucose; C037904:6-deoxyglucose