Purification of the phoU protein, a negative regulator of the pho regulon of Escherichia coli K-12.
Thermally induced transcription of the phoU gene under control of the major leftward promoter, pL, of phage lambda resulted in production of the PhoU protein to compose approximately 5% of the total cell protein. The PhoU protein was present in the cytoplasm in the form of an aggregate. The amino acid composition and N-terminal amino acid sequence of the purified protein confirmed the reading frame established earlier for the phoU gene.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification*; D001692:Biological Transport; D004926:Escherichia coli / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005809:Genes, Regulator; D026901:Membrane Transport Proteins; D010710:Phosphates / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014157:Transcription Factors
D000596:Amino Acids; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D010710:Phosphates; D014157:Transcription Factors; C050770:phoU protein, E coli