[Transparent nutrient medium for culturing Francisella tularensis].
A transparent nutrient medium T (tularemic) for cultivation of F. tularensis was developed on the basis of heart-brain infusion, yeast extract, bactotryptone, technical casamino acids, bactoagar, salts, L-cysteine and glucose. Introduction of an antibiotic (polymyxin B, 100 units/ml) to the medium composition provided selective conditions for growth of F. tularensis. Comparative study of the medium growth properties revealed that medium T by this parameter was not inferior to the Emel'ianova and Antsiferov media and by the yield of the bacterial mass was superior to them. A liquid variant of the medium (broth T) may be successfully used in various experiments requiring broth cultures of F. tularensis (interspecies conjugation, transformation, mutagenesis, etc.). The medium provides the use of wider temperature ranges for cultivation of F. tularensis. Because of transparency the medium may be used in identification of strains and their description by certain biochemical features. Medium T may be also used in studies with field materials (dead rodents, water).
D001431:Bacteriological Techniques; D003470:Culture Media / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005604:Francisella tularensis / Q000254:growth & development*; D013997:Time Factors
D003470:Culture Media
Prozrachnaia pitatel'naia sreda dlia kul'tivirovaniia Francisella tularensis.