Serodiagnosis of New World leishmaniasis by using a genus-specific antigen in enzyme linked immunosorbent assays.
A genus-specific monoclonal antibody (83L-5G9), generated against promastigotes of Leishmania braziliensis panamensis (WRAIR-470), has been used as a ligand in immunochromatography columns for the recovery and purification of the genus-specific antigen. When the purified polypeptide, actually a doublet comprised of a 58 kd and a 31 kd moiety, was used as the antigen in enzyme linked immunosorbent assays (ELISA), it was reactive with 36 of 85 sera from Leishmania patients and with 1 of 49 sera from confirmed cases of Chagas' disease. An additional 25 sera from an unexposed population and five specimens from individuals seropositive for Toxoplasma gondii were nonreactive.
D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007158:Immunologic Techniques; D007891:Leishmania / Q000276:immunology*; D007892:Leishmania braziliensis / Q000276:immunology*; D007896:Leishmaniasis / Q000175:diagnosis*; D013045:Species Specificity
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface