The effects of protein synthesis inhibition, and of mutations rna1.1 and rna82.1, on the synthesis of small RNAs in yeast.
Strains of Saccharomyces cerevisiae have been constructed that possess temperature-sensitive defects in tRNA precursor (pre-tRNA) splicing and which also lack the processing endonuclease that acts at the 3'-terminus of 5 S rRNA and 35 S rRNA precursors (pre-rRNAs). The unspliced pre-tRNAs accumulated by such strains at the nonpermissive temperature are identical in structure to those accumulated by pre-tRNA splicing-defective strains with a functional pre-5 S RNA processing enzyme. The pre-RNA processing activity is therefore not obligatorily involved in maturation of several yeast tRNAs. However, gels of the pulse-labelled RNAs of RNA82+ and rna82.1 strains provide evidence that this enzyme acts upon a few small unstable transcripts that are not 5 S RNA forms. The most prominent of these transcripts on gels was, in wild-type strains, an RNA 145 +/- 2 nucleotides in length.
D003513:Cycloheximide / Q000494:pharmacology; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis; D009154:Mutation; D009694:Nucleic Acid Precursors / Q000096:biosynthesis; D012322:RNA Precursors; D012326:RNA Splicing; D012331:RNA, Fungal / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D005656:Fungal Proteins; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D003513:Cycloheximide; D004722:Endoribonucleases