Fluphenazine-resistant Saccharomyces cerevisiae mutants defective in the cell division cycle.
An fls1 mutant of Saccharomyces cerevisiae, which did not grow in the presence of 30 micrograms of fluphenazine per ml, was isolated. Mutants that were resistant to 90 micrograms of fluphenazine per ml and temperature sensitive for growth were obtained from the fls1 mutant. One fluphenazine-resistance mutation, fsr1, was located near the his7 locus on chromosome II. Growth of the fsr1 mutants at 35 degrees C was arrested after nuclear division. The other group of fluphenazine-resistant mutants, carrying fsr2 mutations, showed Ca2+-dependent growth at 35 degrees C. Growth of the fsr2 mutants at 35 degrees C was arrested at the G2 stage of the cell cycle in Ca2+-poor medium.
D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000502:physiology; D002453:Cell Cycle; D004352:Drug Resistance, Microbial; D005476:Fluphenazine / Q000494:pharmacology*; D005800:Genes, Fungal; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics
D002147:Calmodulin; D005476:Fluphenazine; D002118:Calcium