A method for the quantitative analysis of ELISA data.
A method is described for measuring relationships among virus serotypes. An indirect ELISA was used to obtain antibody-response curves for homologous and heterologous combinations of virus and dilutions of antibody. Data from the proportional response region of each curve were converted to logarithmic values and subjected to a form of regression analysis to derive relative titres for the various combinations of virus and antibody. SDI values were then obtained by calculating the mean difference in relative titres between homologous and reciprocal heterologous combinations. Virus models in two different groups were examined using this technique. Relative titres obtained were consistent and reproducible over several experiments indicating that the technique was capable of reliably discriminating among serotypes which differed by as little as 0.2 SDI.
D004797:Enzyme-Linked Immunosorbent Assay; D009029:Mosaic Viruses / Q000145:classification; D010942:Plant Viruses / Q000145:classification*; D012044:Regression Analysis; D012703:Serotyping