Extraction and isolation by high performance liquid chromatography of uroporphyrin and coproporphyrin isomers from biological tissues.
A simple, rapid procedure has been developed for extraction of uroporphyrin and coproporphyrin isomers from biological tissues. The recoveries of known standards of uroporphyrin I and III and coproporphyrin I and III were performed from liver, kidney, testis, and bone marrow of the rat. The extracted samples were analyzed by high performance liquid chromatography. This method is suitable for the study of drug- and toxicant-induced porphyrias characterized by alterations of the ratios of the I and III isomers of uroporphyrin and coproporphyrin.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000302:isolation & purification*; D007536:Isomerism; D008099:Liver / Q000378:metabolism; D008297:Male; D011166:Porphyrins / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D014578:Uroporphyrins / Q000302:isolation & purification*
D003306:Coproporphyrins; D011166:Porphyrins; D014578:Uroporphyrins; C026971:coproporphyrin III; C031467:coproporphyrin I