Cultured mammary carcinoma cells contain gonadotropin-releasing hormone-like immunoreactivity, GnRH binding sites and chorionic gonadotropin.
Immunoperoxidase staining and radioimmunoassay were used to identify gonadotropin-releasing hormone (GnRH) and chorionic gonadotropin (hCG) in 4 mammary carcinoma cell lines. GnRH-like immunoreactivity was found in all cell lines; 2 lines also contained hCG. Binding of a GnRH agonist was demonstrated by radioreceptor assay in cultured mammary carcinoma cells. In cell column perfusion of the same cells, exogenous GnRH did not stimulate release of hCG.
D001665:Binding Sites; D001943:Breast Neoplasms / Q000032:analysis*; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D010906:Pituitary Hormone-Releasing Hormones / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D011863:Radioimmunoassay
D006063:Chorionic Gonadotropin; D010906:Pituitary Hormone-Releasing Hormones