Carbohydrate interference of complement-dependent cell lysis.
The antibody-mediated cytotoxicity of three autoreactive sera, an allogeneic hyperimmune serum and a xenogeneic hyperimmune serum was abrogated by the presence of either glucosamine, galactosamine, lactulose or lactose. This inhibition could be overcome in a dose-dependent fashion by increasing the amount of complement in the cytotoxicity assay, but not by increasing the amount of antibody. Furthermore, the inhibition was specific for these sugars in that isomers and N-acetylated derivatives were not inhibitory. The results suggest that these sugars directly blocked events of the complement cascade.
D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D002241:Carbohydrates / Q000494:pharmacology*; D003169:Complement Inactivator Proteins; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005688:Galactosamine / Q000494:pharmacology; D005944:Glucosamine / Q000494:pharmacology; D007106:Immune Sera / Q000276:immunology; D007785:Lactose / Q000494:pharmacology; D007792:Lactulose / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB
D001323:Autoantibodies; D002241:Carbohydrates; D003169:Complement Inactivator Proteins; D007106:Immune Sera; D007792:Lactulose; D005688:Galactosamine; D003165:Complement System Proteins; D007785:Lactose; D005944:Glucosamine