Vapor-phase modification of sulfhydryl groups in proteins.
Proteins and peptides are readily and specifically modified at their sulfhydryl groups by the vapors of a mixture of 4-vinylpyridine and tributylphosphine. The phenylthiohydantoin derivative of S-beta-(4-pyridylethyl)cysteine formed during sequence analysis is easily detectable in current identification systems.
D000595:Amino Acid Sequence; D003545:Cysteine / Q000032:analysis*; D006801:Humans; D007328:Insulin / Q000032:analysis; D010720:Phosphines; D011480:Protease Inhibitors; D011506:Proteins / Q000032:analysis*; D011725:Pyridines; D013438:Sulfhydryl Compounds; D014835:Volatilization
D007328:Insulin; D010720:Phosphines; D011480:Protease Inhibitors; D011506:Proteins; D011725:Pyridines; D013438:Sulfhydryl Compounds; C043255:tri-n-butylphosphine; C029351:4-vinylpyridine; D003545:Cysteine