title: Differential in vitro stimulation by naloxone and K+ of luteinizing hormone-releasing hormone and catecholamine release from the hypothalami of intact and castrated rats.
abstract: We compared the effects of an opiate receptor antagonist, naloxone (NAL), on in vitro LHRH and catecholamine release from the medial basal hypothalamus-preoptic area (MBH-POA) of intact and castrated adult male rats. The MBH-POA (six per chamber) were perifused in vitro for 6 h. After 1 h of preincubation, basal LHRH, dopamine (DA), norepinephrine (NE), and epinephrine (E) release were estimated in perifusates collected during the second and third hours. During the fourth hour, chambers were perifused for 30 min with medium alone or medium containing NAL; tissue viability was confirmed during the sixth hour by adding 60 mM KCl to the medium. Tissue samples were weighed at the end of the perifusion and homogenized in 0.1 N HCl for subsequent analyses of LHRH contents. The basal release rate and cumulative hourly LHRH output from the MBH-POA of intact rats was about 3 times that from castrated rats (P less than 0.05). The NAL pulse stimulated LHRH release from the MBH-POA of intact and castrated rats (P less than 0.05); the amount released by the MBH-POA of intact rats was significantly higher than that from castrated rats (P less than 0.05). These differential LHRH release responses reflected the differences in the MBH-POA LHRH concentrations that normally occur between intact and castrated rats and also estimated at the end of the perifusion. In contrast to the LHRH response, the basal release rate and hourly output as well as NAL-induced DA release from the MBH-POA of intact and castrated rats were similar. On the other hand, as in the case of LHRH, basal NE release and hourly output from the MBH-POA of castrated rats were significantly reduced compared to those from the MBH-POA of intact rats (P less than 0.05). In addition, NAL promptly stimulated NE release, and the amount released was higher from the MBH-POA of intact rats (P less than 0.05). The basal amount of E released from the MBH-POA of intact and castrated rats was near or below the level of sensitivity of the assay. However, NAL increased E release from the MBH-POA of both groups of rats, and E output was apparently 2-fold higher from the MBH-POA of intact than castrated rats. Prior perfusion with morphine failed to block NAL-evoked stimulation of LHRH release from the MBH-POA of intact rats.(ABSTRACT TRUNCATED AT 400 WORDS)
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D007033:Hypothalamus, Middle / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009270:Naloxone / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism; D009919:Orchiectomy; D011188:Potassium / Q000494:pharmacology*; D011301:Preoptic Area / Q000378:metabolism; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects
chemical_list: D002395:Catecholamines; D007987:Gonadotropin-Releasing Hormone; D009270:Naloxone; D011188:Potassium; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine