Immunity to Litomosoides carinii in Mastomys natalensis. II. Effects of chemotherapeutically abbreviated and postpatent primary infections on challenges with various stages of the parasite.
Naive Mastomys natalensis, Litomosoides carinii-infected M. natalensis at a postpatent stage of the infection and L. carinii-infected M. natalensis treated chemotherapeutically with furazolidone (FUR), FUR and diethylcarbamazine (FUR/DEC) or amoscanate (AMOS) were challenged by either injection or implantation of 40 third stage larvae (L3, s.c.), 40 fourth stage larvae (L4, 16 days old, i.p.), 20 male and 20 female preadult worms (36 days old, i.p.), 12 adult female worms (i.p.) or 6 X 10(6) microfilariae/kg (i.v.). Microfilaraemia in animals challenged at a postpatent stage (independent of the kind of challenge), was either totally suppressed or at least greatly reduced. Necropsy of L3-challenged animals showed that neither the length of the worms nor their content of morphologically intact, intrauterine stages was affected. Infected, treated animals challenged with developing stages (L3, L4 and preadult worms) showed reduced levels of microfilaraemia (by up to 75%). Dissection of AMOS-treated, L3-challenged animals showed that both the developmental rate and the fertility of the worms were affected. Microfilaraemia was also reduced after implantation of adult worms into treated animals. This was independent of the interval between treatment and challenge (44-150 days) except in animals challenged 10 days after AMOS-treatment, which showed no difference from naive controls. However, infected, treated M. natalensis, cotton rats and gerbils did not develop immunity against intravenously injected blood microfilariae.
D000818:Animals; D003411:Arvicolinae; D004049:Diethylcarbamazine / Q000494:pharmacology; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000494:pharmacology; D005368:Filariasis / Q000188:drug therapy / Q000276:immunology* / Q000469:parasitology; D005369:Filaricides / Q000494:pharmacology; D005370:Filarioidea / Q000187:drug effects / Q000276:immunology*; D005664:Furazolidone / Q000494:pharmacology; D005849:Gerbillinae; D017879:Isothiocyanates; D008842:Microfilariae / Q000187:drug effects / Q000276:immunology; D009115:Muridae; D013861:Thiocyanates / Q000494:pharmacology
D005369:Filaricides; D017879:Isothiocyanates; D013861:Thiocyanates; D005664:Furazolidone; D004159:Diphenylamine; D004049:Diethylcarbamazine; C012391:amoscanate