A specific histochemical marker (lectin Ricinus communis agglutinin-1) for normal human microglia, and application to routine histopathology.
Microglia were demonstrated in paraffin-embedded human nervous tissues with an avidin-biotin peroxidase method and Ricinus communis agglutinin-1 (RCA-1). Specific staining was observed in cell bodies and processes of microglia. Although endothelial cells and blood cells reacted with RCA-1, they were easily distinguished morphologically from microglia. Astrocytes, oligodendrocytes, and neurons did not react with RCA-1. These results suggest that RCA-1 can be used as a new histochemical marker for microglia in normal human brain.
D000293:Adolescent; D000328:Adult; D001360:Avidin; D001710:Biotin; D001921:Brain / Q000166:cytology*; D002648:Child; D002675:Child, Preschool; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D037121:Plant Lectins
D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D001360:Avidin; D001710:Biotin