[Possibility of detecting the matrix protein of the influenza virus in intact and disrupted virions by immunoenzyme analysis and immune electron microscopy].
ELISA and immune electron microscopy were used to study possible causes of the detection of antigenic reactivity of influenza virus matrix protein in purified virus suspension directly adsorbed on polystyrene. No interaction of antibody to M protein with the surface of virus and subvirus particles was observed. The process of virus sorption on polystyrene for a long period was shown not to lead to disruption of intact virus particles, and the detection of the internal matrix protein in preparations of purified influenza virus was due only to the presence of partially or completely destroyed virions in the viral suspension.
D000042:Absorption; D000914:Antibodies, Viral / Q000302:isolation & purification; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D009980:Influenza A virus / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology; D014771:Virion / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins
Issledovanie vozmozhnosti vyiavleniia matriksnogo belka virusa grippa v intaktnykh i razrushennykh virionakh s ispol'zovaniem immunofermentnogo analiza i immunnoĭ élektronnoĭ mikroskopii.