Nucleoprotein complexes that regulate gene expression in adipocyte differentiation: direct participation of c-fos.
Adipocyte differentiation is accompanied by the transcriptional activation of many new genes, including a putative lipid-binding protein termed adipocyte P2 (aP2). The aP2 gene contains a regulatory element (FSE2) 124 bases 5' to its start of transcription. This element binds nuclear factors in sequence-specific and differentiation-dependent fashion as determined by altered mobility in gel retardation assays. Deletion analysis of promoter-linked transfection assays and competition of these constructions in cells with a synthetic FSE2 element suggest that trans-acting factors bind to this region and act as negative regulators of aP2 gene activity in preadipocytes. c-fos appears to participate directly in this nucleoprotein complex, as demonstrated by the ability of antibodies to c-fos to disrupt specific binding of factors to the FSE2 sequence but not to factor-binding sequences from several other genes. Antibodies to c-fos specifically immunoprecipitate protein complexes covalently bound to FSE2 DNA via UV cross-linking.
D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002454:Cell Differentiation; D002874:Chromosome Mapping; D011416:Complement Factor D; D004268:DNA-Binding Proteins / Q000502:physiology; D010450:Endopeptidases / Q000235:genetics; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005786:Gene Expression Regulation; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics; D051379:Mice; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009698:Nucleoproteins / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D011519:Proto-Oncogenes; D012697:Serine Endopeptidases; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology*
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009698:Nucleoproteins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D005993:Glycerolphosphate Dehydrogenase; D010450:Endopeptidases; D012697:Serine Endopeptidases; D011416:Complement Factor D; C493491:complement factor D, mouse