Delta 1-pyrroline-5-carboxylate dehydrogenase in the bovine ciliary body and iris.
Delta 1-pyrroline-5-carboxylate dehydrogenase extracted from the combined ciliary body and iris of bovine eyes was studied biochemically. The enzyme was purified 120-fold. The partially purified enzyme had broad optimum at pH 8.0. Apparent Km values for DL-delta 1-pyrroline-5-carboxylate and NAD were 0.14 and 0.18 mM, respectively. The enzyme activity was strongly inhibited by GABA, proline, hydroxyproline, and glutamine.
D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002924:Ciliary Body / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007498:Iris / Q000201:enzymology*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*
D000596:Amino Acids; D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors