Immunoelectron microscopic localization of progesterone receptor in the chick oviduct.
A peroxidase-anti-peroxidase (PAP) method using polyclonal anti-PR antibodies was used to localize progesterone receptor (PR) electron microscopically in the chick oviduct. The immunoreaction precipitate indicating PR was localized inside the nuclei of epithelial, glandular and stromal cells. In the estrogen withdrawn oviduct cytoplasmic immunoreaction precipitate was not seen. Inside the nucleus unoccupied PR was localized mainly like the heterochromatin. As visualized by the PAP technique, the localization of PR was not systematically changed after progesterone administration. In conclusion, we suggest that progesterone receptor in the chick oviduct is an intranuclear protein.
D000818:Animals; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D010057:Oviducts / Q000378:metabolism* / Q000648:ultrastructure; D011980:Receptors, Progesterone / Q000378:metabolism*
D011980:Receptors, Progesterone