A simple procedure for isolation of microsomes from human placenta.
A method for rapid isolation of human placenta microsomes, which does not require facilities for ultracentrifugation was described. Such microsomes were compared with microsomes prepared by conventional ultracentrifugation technique. Both microsomal preparations were tested for protein, RNA and phospholipid content as well as for sulphohydrolase activities and glucose-6-phosphatase activity. The degree of contamination with other subcellular particles were tested as well. The data presented showed that microsomal proteins precipitated at pH 5.3 may be conveniently used for preparative separation of microsomal enzymes.
D002458:Cell Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008861:Microsomes / Q000032:analysis / Q000648:ultrastructure*; D010920:Placenta / Q000032:analysis / Q000648:ultrastructure*; D011247:Pregnancy; D014461:Ultracentrifugation