Ibuprofen as an antagonist of inhibitors of fibrinolysis in wound fluid.
Fibrin plate assay revealed that rat serum and wound fluid harvested from seven day subcutaneously implanted wound chambers prevented fibrinolysis. Samples of wound fluid from one to four hour burns displayed greater inhibiting activity than unburned or 24 hour old burns. Ibuprofen, a non-steroid anti-inflammatory drug, reversed the blocking of fibrinolysis in wound fluid, but it had no action on rat serum. The activity of ibuprofen appears unrelated to the synthesis of prostanoids. Fractionation of wound fluid and serum by column chromatography showed differences in elutions of inhibitors of fibrinolysis. Serum fractions having molecular weights greater than 60,000 prevented fibrinolysis and they were unaffected by the addition of ibuprofen. Fractionations of wound fluid produced a number of inhibitors, some of which had molecular weights of approximately 40,000. This inhibitor(s) was not detected in serum and was reversed by adding ibuprofen. Wound fluid has a fibrinolytic inhibitor which differs from that in the circulatory system, and which may be critical to the vascular changes of burn trauma.
D000818:Animals; D000933:Antifibrinolytic Agents / Q000037:antagonists & inhibitors; D002056:Burns / Q000378:metabolism; D005110:Extracellular Space / Q000032:analysis / Q000502:physiology; D005337:Fibrin / Q000187:drug effects; D005342:Fibrinolysis / Q000187:drug effects*; D007052:Ibuprofen / Q000494:pharmacology*; D008297:Male; D008722:Methods; D051381:Rats; D011919:Rats, Inbred Strains; D014945:Wound Healing
D000933:Antifibrinolytic Agents; D005337:Fibrin; D007052:Ibuprofen