Inhibition of pyrimidine dimer excision in ultraviolet-irradiated Escherichia coli overproducing RecA protein.
Escherichia coli Br Hcr+ cells transformed with the recombinant multicopy plasmid pBR322 carrying recA gene contain increased amounts of RecA protein. When these cells were UV-irradiated, excision of pyrimidine dimers was reduced by about 50%. It is suggested that the damaged DNA strands may be coated with RecA protein which makes them insensitive to the action of the uvrABC excision nuclease.
D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects*; D010957:Plasmids; D011740:Pyrimidine Dimers / Q000378:metabolism*; D011938:Rec A Recombinases / Q000502:physiology*; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D011938:Rec A Recombinases