A system for the analysis of expression signals in Aspergillus.
To analyse gene expression signals in Aspergillus we have constructed a set of integration vectors each of which contains in front of the Escherichia coli 'lacZ gene a unique BamHI site in one of the three possible translational reading frames and the A. nidulans argB gene as a selection marker. The vectors allow in-phase translational fusion of any gene to 'lacZ. After transformation of an A. nidulans argB strain, the vectors integrate with a high percentage at the argB locus of the genome, as a single copy. The insertion of the fusion genes at the argB locus assures the constancy of influences of the chromosomal environment on gene expression.
D001233:Aspergillus nidulans / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005822:Genetic Vectors; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D011993:Recombinant Fusion Proteins