title: Immunocytochemical localization of neuropeptide Y-like immunoreactivity in adrenergic and non-adrenergic neurons of the rat gastrointestinal tract.
abstract: The immunocytochemical location of neuropeptide Y (NPY)-like immunoreactivity (LI) within the neuronal structures of the rat gastrointestinal (GI) tract was investigated with the indirect immunofluorescence method. NPY immunoreactive neurons were found throughout all regions of the GI tract with the largest number in the duodenum. NPY immunoreactive perikarya were mainly located in the submucosal ganglia. NPY labeled processes were extensively seen in the submucosal and myenteric plexuses, smooth muscles, muscularis mucosa, mucosa and surrounding blood vessels. Following 6-hydroxydopamine (6-OHDA) treatment, NPY immunoreactive nerve fibers around blood vessels disappeared completely and the reactive fibers in other regions were reduced in number. NPY immunoreactive nerve cell bodies in the ganglionic plexuses, however, were not affected by 6-OHDA treatment. Serial sections of the coeliac ganglion showed that NPY-LI was present in cell bodies which also displayed tyrosine hydroxylase (TH) immunoreactivity. Our results suggest that NPY is abundantly contained in both adrenergic and non-adrenergic neurons of the gut and may play an important role in the regulation of the GI tract.
mesh_terms: D000320:Adrenergic Fibers / Q000032:analysis*; D000818:Animals; D004064:Digestive System / Q000294:innervation*; D004947:Esophagus / Q000294:innervation; D005455:Fluorescent Antibody Technique; D005728:Ganglia, Sympathetic / Q000032:analysis; D006651:Histocytochemistry; D007422:Intestines / Q000294:innervation; D008297:Male; D009478:Neuropeptide Y / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000294:innervation
chemical_list: D009478:Neuropeptide Y