Electron microscopic localization of a fucose-binding protein in acrosome reacted boar spermatozoa by the fucosyl-peroxidase-gold method.
In this study we have examined the behaviour and the localization of the fucose-binding protein (FBP) in boar spermatozoa during ionophore induced acrosome reaction (AR) by means of normal TEM and specimen preparation in toto. During early stages of AR the FBP is first localized at the border between equatorial segment and anterior acrosome. With the propagation of the AR the FBP is dramatically expressed and visible over the entire surface of the acrosome and equatorial segment. TEM pictures of this stages show that the FBP is associated with the OAM. At later stages of AR, when acrosomal ghost formation occur, the FBP is associated with the acrosomal ghost, and equatorial segment and to a very low degree also with the IAM. It is concluded from this data that the FBP is responsible for the specific binding of the ghost-sperm unit to the zona pellucida.
D000177:Acrosome / Q000032:analysis / Q000502:physiology / Q000648:ultrastructure*; D000818:Animals; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D013094:Spermatozoa / Q000032:analysis / Q000648:ultrastructure*; D013552:Swine
D037102:Lectins; C027112:fucose-binding lectin