title: Repression of meiotic crossing over by a centromere (CEN3) in Saccharomyces cerevisiae.
abstract: The location of the centromere of chromosome III (CEN3) of Saccharomyces cerevisiae has been altered by means of transformation. The frequency of meiotic crossing over in the CEN3-PGK1 and LEU2-CEN3 intervals increases approximately 1.5- and fourfold, respectively, when CEN3 is repositioned at HIS4. The centromere-distal HIS4-LEU2 region experiences a three- to fivefold decrease in the frequency of meiotic exchange when CEN3 is repositioned at HIS4. The inhibition of meiotic crossing over is conferred by a 627-base-pair fragment of CEN3 DNA and is not dependent on the orientation of CEN3 relative to the rest of chromosome III.
mesh_terms: D002503:Centromere / Q000502:physiology*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000502:physiology*; D003434:Crossing Over, Genetic; D005838:Genotype; D008540:Meiosis; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D013489:Suppression, Genetic