Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D046911:Macromolecular Substances; D009694:Nucleic Acid Precursors / Q000302:isolation & purification; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000302:isolation & purification; D012342:RNA, Small Nuclear / Q000302:isolation & purification; D012261:Ribonucleoproteins / Q000235:genetics / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D046911:Macromolecular Substances; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins