title: Purification and characterization of a proline-rich secretory protein that is a precursor to a structural protein of an insect spermatophore.
abstract: The spermatophore or sperm sac of Tenebrio molitor (yellow mealworm beetle) is an acellular structure composed mostly of structural proteins, termed spermatophorins. The proteins are derived from the bean-shaped accessory reproductive glands of the male and are assembled into the multilayered structure within the ejaculatory duct. Homogenates of the secretory plug from this gland were used as immunogens for the production of monoclonal antibodies, including one identified as PL 21.1 which recognizes an antigen in the gland and the spermatophore. With the aid of gel filtration and immunoaffinity chromatography with a PL 21.1, we isolated a glandular secretory protein that is a precursor to a spermatophorin with similar electrophoretic mobility. On native polyacrylamide gels, the antigen from gland homogenates has an apparent molecular mass of 370 kDa. On sodium dodecyl sulfate gels, the antigen from the gland and that from the spermatophore have apparent molecular masses of 23 kDa. According to immunoblots of sodium dodecyl sulfate gels, the 23-kDa glandular antigen is organ-specific and adult-specific. By immunocytochemistry with PL 21.1, we found the antigens to be restricted to secretory vesicles of only one cell type in the gland and to a discrete layer in the outer wall of the spermatophore. The 23-kDa secretory antigen is distinguished by being high in glutamic acid/glutamine (15.4%) and in proline (25.2%).
mesh_terms: D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D007120:Immunochemistry; D007301:Insect Hormones / Q000276:immunology / Q000302:isolation & purification*; D019476:Insect Proteins; D008297:Male; D011392:Proline / Q000032:analysis; D011498:Protein Precursors / Q000276:immunology / Q000302:isolation & purification*; D013093:Spermatogonia / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D013094:Spermatozoa / Q000032:analysis*; D013712:Tenebrio
chemical_list: D000941:Antigens; D007301:Insect Hormones; D019476:Insect Proteins; D011498:Protein Precursors; C052125:SP23 protein, Tenebrio molitor; D011392:Proline