title: [Long-term stability of enzymes in solution].
abstract: Two proteases (thermitase, a thermostable serine protease from Thermoactinomyces vulgaris and subtilisin Carlsberg) and one non-proteolytic enzyme (urate oxidase from Penicillium spp.) were used for revealing the main influences leading to the inactivation of enzyme preparations during their long-time storage at low temperatures. The temperature dependences (0 degree C-60 degrees C) of inactivation resulted in a linear Arrhenius-plot for each of the three native enzymes as well as in the presence of all stabilizing substances tested. Therefore, a method is available which shortens the time considerably needed for the experiments aimed at the discovery of substances stabilizing enzymes under storage conditions (i.e. long time at low temperatures). Because of the linear Arrhenius-plots potential stabilizers can be tested experimentally at suitable higher temperatures and one can extrapolate on their influence on the enzyme at storage temperatures (0 degree C-10 degrees C). By using this method effective combinations of stabilizers for urate oxidase were found, and possible reasons of their stabilizing influence on the enzyme are discussed.
mesh_terms: D003080:Cold Temperature; D010450:Endopeptidases; D004795:Enzyme Stability / Q000187:drug effects; D007700:Kinetics; D012697:Serine Endopeptidases; D013381:Subtilisins; D014503:Urate Oxidase
chemical_list: D014503:Urate Oxidase; D010450:Endopeptidases; D012697:Serine Endopeptidases; D013381:Subtilisins; C022728:thermitase
vernacular_title: Untersuchungen zur Langzeitstabilität von Enzymen in Lösung.