title: Repair of severed peripheral nerves: comparison of the "de Medinaceli" and standard microsuture methods.
abstract: The return of function following transection of the rat sciatic nerve has been assessed after repair by either standard microsurgical techniques (i.e., the use of microsutures to coapt the severed ends) or the new repair technique introduced by de Medinaceli and coworkers. The regeneration after transection was compared with that following sciatic nerve crush, i.e., a lesion in which the return of function is near optimal. Return of function was monitored serially using walking track analysis (i.e., the sciatic functional index, which indicates overall functional performance), the ability to spread the toes (which indicates intrinsic function in the foot), and the determination of muscle twitch tension of the middle digit. Function in the nerve crush group returned to within the normal range by 53 days, but function in the transection and repair groups did not return to normal before the rats were perfused at 85 postoperative days. However, the function regained when nerves were repaired with the de Medinaceli technique was significantly superior to that regained after repair with microsutures. Histological examination of the repair site (at 85 days) revealed that the regenerated nerve fibers in the de Medinaceli group crossed the site of anastomosis in a relatively orderly fashion, whereas they were more randomly arranged when microsutures were used. However, there was no statistical difference between the two groups in the number of regenerated fibers present in the distal stump. The increase in function in the de Medinaceli group may therefore arise primarily from an increase in the proportion of regenerating fibers which reach appropriate targets.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D002452:Cell Count; D003714:Denervation; D008297:Male; D008866:Microsurgery / Q000379:methods*; D009043:Motor Activity; D009132:Muscles / Q000502:physiology; D009409:Nerve Crush; D009413:Nerve Fibers, Myelinated / Q000502:physiology; D009416:Nerve Regeneration; D010525:Peripheral Nerves / Q000502:physiology / Q000601:surgery*; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000502:physiology; D013536:Suture Techniques; D013997:Time Factors; D014034:Toes / Q000502:physiology