Insulin-like effects in the rat of the purified growth factor from Spirometra mansonoides plerocercoids.
The acute effects of injections of the human growth hormone-like factor purified from plerocercoids of the tapeworm Spirometra mansonoides on carbohydrate, lipid, and protein metabolisms were determined in intact rats. Male rats were injected ip with saline, insulin, or various doses of partially purified PGF. The rats injected with insulin had significantly reduced serum glucose concentrations but no dose of PGF caused a change in serum glucose levels. Insulin and PGF stimulated [14C]glucose and [14C]leucine oxidation to 14CO2 in adipose tissue and muscle and increased incorporation of both [14C]glucose carbons into lipids and [14C]leucine into protein in fat and muscle. The responses to PGF were dose-dependent and persisted after 3 hr of incubation in vitro. Injections of naloxone prior to injecting PGF to block the stress response did not prevent the stimulation of insulin-like responses by PGF. Therefore, PGF has intrinsic insulin-like activities in normal male rats.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002589:Cestoda; D005947:Glucose / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013146:Spirometra
D006133:Growth Substances; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; C043871:plerocercoid growth factor; D013006:Growth Hormone; D007930:Leucine; D005947:Glucose