Stability study of prostacyclin in solution.
The proton-catalyzed hydrolytic decomposition of prostacyclin was assayed by UV spectroscopic and HPLC methods. Changes of the absorbance in aqueous solutions were measured at 220 nm. HPLC studies were performed on C18 column using NH4Cl (0.03 mol X l-1)/NH4OH (0.03 mol X l-1)/methanol (1 : 1 : 4) eluent. Temperature and pH dependence of the hydrolytic process was determined. Effects of alcohol and glycols on the degradation rate were studied.
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D011464:Epoprostenol / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D012996:Solutions; D011464:Epoprostenol