A sensitive and class specific solid phase enzyme immunoassay for anti-DNA autoantibodies in supernatants of lymphocyte cultures and human hybridomas.
A solid phase enzyme immunoassay using methylated bovine serum albumin (BSA)-precoated and DNA-coated microtiter plates was developed for the detection of IgG and IgM anti-DNA autoantibodies in the supernatants of lymphocyte cultures and hybridomas. In patients with systemic lupus erythematosus (SLE) the significantly raised IgG anti-DNA antibody synthesis indicates that preactivated anti-DNA clones circulate in the peripheral blood. This was associated with the detection of anti-DNA antibodies in the sera. The screening of 53 supernatants from human x mouse hybridomas showed an antibody to denatured DNA in 16 supernatants.
D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D004247:DNA