title: Effects of preparative technique on the rate of adoption of crawling-like movements by polymorphonuclear leukocytes.
abstract: Various component steps of several "standard" techniques for separating polymorphonuclear leukocytes (PMN) from whole blood were tested for their effects on the rate at which these cells exhibit crawling-like movements when suspended in 100% plasma. The rate was depressed by excessive centrifugation, excessive agitation, erythrocyte lysis techniques, prolonged preparation time and one type of separation medium used. More than 95% of PMN in all preparations excluded the dye Trypan blue. The rate at which PMN exhibit crawling-like movements in plasma could be a sensitive index of the viability of these cells.
mesh_terms: D002465:Cell Movement; D002498:Centrifugation; D003470:Culture Media; D003584:Cytological Techniques; D004912:Erythrocytes / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology*; D013696:Temperature; D014343:Trypan Blue
chemical_list: D003470:Culture Media; D014343:Trypan Blue