Immunohistochemical diagnosis of toxoplasmosis in fetuses and fetal membranes of sheep.
A peroxidase antiperoxidase technique was developed for visualizing intact Toxoplasma gondii and its antigenic residues in sections of formalin-fixed, paraffin-embedded tissues from fetal membranes recovered from ewes with induced toxoplasmosis. The peroxidase antiperoxidase method was specific and sensitive, allowing the detection of T gondii antigen even in severely decomposed tissues. Toxoplasma gondii infection was most commonly demonstrated in the cotyledons, heart, lungs, brain, and skeletal muscles of the fetus.
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D005321:Extraembryonic Membranes / Q000469:parasitology; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis / Q000662:veterinary*; D005333:Fetus / Q000469:parasitology; D007124:Immunoenzyme Techniques; D051379:Mice; D010920:Placenta / Q000469:parasitology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000662:veterinary*; D012756:Sheep / Q000469:parasitology; D012757:Sheep Diseases / Q000151:congenital / Q000175:diagnosis*; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000151:congenital / Q000175:diagnosis*
D000953:Antigens, Protozoan