The inhibitory effect of dithiothreitol on the assembly of the filamentous phage fd.
Assembly of the filamentous phage fd is preceded by the formation of a complex between the viral single-stranded (ss) DNA and the virally coded gene 5 protein (gene 5 protein-ssDNA complex). The presence of 5 mM dithiothreitol in the growth medium prevents phage production; however, phage infection, phage DNA replication and phage genome expression are still observed. In contrast, the gene 5 protein-ssDNA complex is not formed in the presence of dithiothreitol in vivo, although the complex is not affected by the disulfide reducing agent in vitro. Furthermore, host lipid composition is altered by growth in the presence of dithiothreitol. The zwitterionic lipid, phosphatidylethanolamine, increases while the cationic phospholipid content, cardiolipin and phosphatidylglycerol, decreases. This suggests a role for lipids or membranous structures in the process of gene 5 protein-ssDNA complex formation.
D002308:Cardiolipins / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D002308:Cardiolipins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D014764:Viral Proteins; D004229:Dithiothreitol