title: Computer controlled affinity chromatographical purification of soluble Plasmodium falciparum antigens from supernatants of in vitro cultures.
abstract: Affinity chromatographic procedures are difficult to scale up from the analytical to the preparative level when the ligand used for purification is a limiting factor. A versatile, computer-controlled affinity chromatographic system is described which permits automatic repetition of the purification process and sophisticated control functions based on the ultra-violet absorbance of fluid passing through the affinity column. The system has been used for automation and scaling up of the purification of Plasmodium falciparum exoantigens.
mesh_terms: D000953:Antigens, Protozoan / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003201:Computers; D066298:In Vitro Techniques; D010963:Plasmodium falciparum / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan