Monoclonal antibodies to Mojave toxin and use for isolation of cross-reacting proteins in Crotalus venoms.
Hybridomas secreting monoclonal antibodies against Mojave toxin were established. The antibodies were used for identifying cross-reacting proteins in individual C. s. scutulatus and other Crotalus venoms and to isolate Mojave toxin. The antibodies recognized five bands with a pI range from 5.1 to 6.1 in immunoblots of electrofocused crude venom and Mojave toxin purified by immunoaffinity chromatography. The specificity of the antibodies was for the basic subunit of the toxin, which resolved into four bands of pI between 9.3 and 9.6. Individual C. s. scutulatus venoms of snakes from Texas and southern Arizona had multiple bands with pI's ranging from 4.9 to 6.3. Cross-reacting proteins were also recognized by the antibodies in the electrophoresed venoms of C. basiliscus, C. d. durissus, C. d. terrificus, C. h. horridus and C. v. concolor, and may be isolated by immunoaffinity chromatography with the monoclonal antibodies.
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000032:analysis* / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D007158:Immunologic Techniques; D009498:Neurotoxins / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; C018107:Mojave toxin; D009498:Neurotoxins