[In vitro effects of E. coli endotoxin on the membrane permeability and substrate transport of isolated rat liver mitochondria].
Incubation of freshly isolated rat liver mitochondria with E. coli endotoxin resulted in an increased inner membrane permeability for K+ and Cl- ions. This effect was not prevented by addition of the synthetic antioxidant butylated hydroxytoluene. The carrier-mediated transport rates of phosphate, pyruvate, citrate and reducing equivalents via the malate-aspartate shuttle were not altered significantly by endotoxin. Therefore, the endotoxin-mediated impairment of mitochondrial respiration and oxidative phosphorylation could not be attributed to a decrease in transport capacities through the inner mitochondrial membrane.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010539:Permeability; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002712:Chlorides; D004731:Endotoxins; D002084:Butylated Hydroxytoluene; D011188:Potassium
In vitro-Effekt von E. coli-Endotoxin auf Membranpermeabilität und Substrattransport isolierter Rattenlebermitochondrien.