Determination of bacterial cell volume with the Coulter Counter.
Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., electronic measurement with a Coulter Counter-Analyzer system and biophysical measurement of the total volume and number of cells in sedimented cell pellets. Within experimental errors, the methods gave the same mean cell volumes.
D001431:Bacteriological Techniques / Q000295:instrumentation*; D055592:Biophysical Phenomena; D001703:Biophysics; D002452:Cell Count / Q000295:instrumentation*; D004926:Escherichia coli / Q000648:ultrastructure*