title: Sequence of the M1-2 region of killer virus double-stranded RNA.
abstract: A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D005800:Genes, Fungal; D005814:Genes, Viral; D012330:RNA, Double-Stranded / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013698:Templates, Genetic; D014780:Viruses / Q000235:genetics*
chemical_list: D012330:RNA, Double-Stranded; D004247:DNA