Deep-freezing versus 4 degrees preservation of avascular osteocartilaginous shell allografts in rats.
Osteocartilaginous allografts (distal femurs of rats) were stored at 4 degrees for six, 12, 24, and 48 hours and at -80 degrees for five days and then evaluated for viability of the bone and cartilage. Storage at 4 degrees for 12 or 24 hours had little effect on cartilage viability but decreased bone viability to 40% and 10% of controls, respectively. Storage at -80 degrees for five days resulted in nonviable bone in all cases but showed an either/or response of cartilage, with high viability in two cases and nonviability in the other eight cases. In a second set of experiments, femurs from rats were stored in situ at 4 degrees for 12 or 24 hours or were harvested and stored at -80 degrees for five days, after which they were transplanted into rats of a different strain. The antibody response to each set of femurs was measured at two, six, and 12 weeks after operation. The 4 degrees storage resulted in a moderately decreased immunogenicity, whereas the storage at -80 degrees resulted in significantly reduced immunogenicity.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D016025:Bone Transplantation; D001842:Bone and Bones / Q000276:immunology; D002356:Cartilage / Q000276:immunology / Q000637:transplantation*; D005269:Femur / Q000276:immunology / Q000637:transplantation; D005615:Freezing; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D000906:Antibodies