UV response of the temperature-conditional rad 54 mutant of the yeast Saccharomyces cerevisiae.
The survival of the yeast mutant rad 54-3, which is temperature-conditional for the repair of double-strand breaks, was measured after exposure to UV-light (254 nm) and incubation at 23 degrees C and 36 degrees C. It was found that survival was drastically reduced with incubation at the restrictive temperature. Temperature-shift experiments indicated that repair of UV-induced damage which is controlled by the rad 54 gene proceeds with a half-value-time of about 7 h.
D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005800:Genes, Fungal; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000528:radiation effects*; D013696:Temperature; D014466:Ultraviolet Rays