Identification of Mycobacterium bovis, using miniaturized thin-layer chromatography and morphologic characteristics.
Lipid analysis was performed on 204 isolates of mycobacteria, using miniaturized thin-layer chromatography (MTLC). A previously described lipid component was found in 92 (96.8%) of 95 isolates of Mycobacterium bovis and in 2 (1.8%) of 109 isolates of other mycobacteria. Using MTLC, an isolate could be identified as M bovis 3 to 4 weeks earlier than by use of conventional biochemical techniques. Examination of isolates for colonial and cellular morphologic characteristics was a useful adjunct in the identification of M bovis by use of MTLC.
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D008055:Lipids / Q000032:analysis*; D009163:Mycobacterium bovis / Q000032:analysis / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification
D008055:Lipids