Immunolocalization of glyceraldehyde-3-phosphate dehydrogenase, hexokinase, and carboxypeptidase Y in yeast cells at the ultrastructural level.
We have developed a simple and effective method to embed whole yeast cells in Lowicryl resins with excellent ultrastructural and antigenic preservation. Using affinity-purified antibodies eluted from electrophoretically separated proteins transferred to nitrocellulose, we have shown by immunoelectron microscopy that two glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase and hexokinase, are present in the cytoplasm and the nucleus. Carboxypeptidase Y is localized in the yeast vacuole. These results agree with earlier localization studies based on subcellular fractionation.
D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D000941:Antigens / Q000276:immunology; D002268:Carboxypeptidases / Q000032:analysis* / Q000276:immunology; D043402:Cathepsin A; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis* / Q000276:immunology; D006046:Gold; D006593:Hexokinase / Q000032:analysis* / Q000276:immunology; D007159:Immunologic Tests; D008854:Microscopy, Electron; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D013205:Staphylococcal Protein A; D014617:Vacuoles / Q000201:enzymology
D000906:Antibodies; D000941:Antigens; D029701:Saccharomyces cerevisiae Proteins; D013205:Staphylococcal Protein A; D006046:Gold; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006593:Hexokinase; D002268:Carboxypeptidases; D043402:Cathepsin A; C499283:PRC1 protein, S cerevisiae; C022633:serine carboxypeptidase