Analysis and utilization of the preprotoxin gene encoded in the M1 double-stranded RNA of yeast.
The yeast type 1 toxin is a secreted protein which, along with immunity to the toxin, is encoded by an encapsidated double-stranded RNA (dsRNA) as a precursor protein, the preprotoxin. This chapter reviews recent work that has led to sequencing of complementary DNA (cDNA) copies of the preprotoxin gene, expression of toxin and toxin-immunity from cDNA, and use of the cDNA to effect secretion from yeast of a bacterial cellulase.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005796:Genes; D005800:Genes, Fungal; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D055646:Killer Factors, Yeast; D009183:Mycotoxins; D012330:RNA, Double-Stranded; D004247:DNA