Statement on the nomenclature of dog C4 allotypes.
As a collaborative work of three laboratories the polymorphism of the canine fourth complement component (C4) was studied in a total of 131 unrelated dogs from different breeds and mongrels. Using high voltage electrophoresis followed by an immunoblotting technique, we detected eight distinct variants. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of canine C4 showed an additional heterogeneity of the alpha and gamma chains which resulted in a total of 11 variants in the population studied. So that more precise information concerning the respective C4 allotypes will be available, a nomenclature is proposed designating not only the migration pattern of the C4 variants in agarose gels but also the heterogeneity of the C4 chains observed in SDS-PAGE.
D000483:Alleles; D000818:Animals; D003181:Complement C4 / Q000235:genetics*; D004285:Dogs / Q000235:genetics / Q000276:immunology*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D009626:Terminology as Topic
D003181:Complement C4; D046911:Macromolecular Substances