title: Isolation and characterization of a substitution mutation in the ompR gene of Salmonella typhimurium LT2.
abstract: The expression of the genes ompC and ompF encoding major outer membrane proteins is dependent on the ompR-envZ operon. Here we describe the isolation and characterization of an ompR mutation, a single-base-pair change, that results in an Arg-to-Cys substitution. When present in multiple copies, the mutant allele conferred a dominant OmpC- OmpF+ phenotype. Furthermore, the mutant allele exhibited allele-specific negative complementation with other ompR mutations. This ability, together with its dominant character, suggested that the OmpR protein is capable of multimerization.
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins