title: Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells.
abstract: A polypeptide termed oncostatin M, which inhibits the replication of A375 melanoma and other human tumor cells, but not normal human fibroblasts, has been isolated from serum-free supernatants of U-937 histiocytic lymphoma cells that have been induced to differentiate into macrophage-like cells following treatment with the phorbol ester phorbol 12-myristate 13-acetate. No such growth inhibitory activity is detected in the supernatant of untreated U-937 cells, indicating that the protein is induced or increased in expression in the phorbol ester-induced differentiated cells. Oncostatin M is stable between pH 2 and 11 and after heating for 1 hr at 56 degrees C but is not stable at 90 degrees C. Purification of oncostatin M has been achieved by gel chromatography and reversed-phase HPLC, using sequentially acetonitrile and n-propanol in the presence of aqueous trifluoroacetic acid. The apparent molecular weight of oncostatin M is approximately 18,000, as determined by gel chromatography, and 28,000, as determined by polyacrylamide gel electrophoresis. The amino-terminal amino acid sequence of the purified polypeptide has been determined. No substantial sequence homology between oncostatin M and other proteins was found, including other tumor cell inhibitory proteins produced by mononuclear cells. Oncostatin M, therefore, appears to represent a distinct cell growth regulator.
mesh_terms: D000595:Amino Acid Sequence; D001688:Biological Products / Q000302:isolation & purification* / Q000494:pharmacology; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines; D005347:Fibroblasts / Q000166:cytology; D006131:Growth Inhibitors / Q000302:isolation & purification*; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000032:analysis* / Q000503:physiopathology; D008264:Macrophages / Q000502:physiology; D008546:Melanoma, Experimental / Q000473:pathology; D008970:Molecular Weight; D053683:Oncostatin M; D010455:Peptides / Q000302:isolation & purification*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D001688:Biological Products; D016207:Cytokines; D006131:Growth Inhibitors; C508351:OSM protein, human; D010455:Peptides; D053683:Oncostatin M; D013755:Tetradecanoylphorbol Acetate