Fluorescence detection of some nitrosoamines in high-performance liquid chromatography after post-column reaction.
A selective fluorescence detection method for the determination of some N-nitrosoamines after post-column reaction has been developed for reversed-phase liquid chromatography. The N-nitroso compounds are analyzed by allowing their hydrolysis products to react with the oxidizing species Ce4+ to produce the fluorescent ion Ce3+. The detection limit for this method is at the ppb (the American billion, 10(9)) level with a linear dynamic range of 2-3 orders of magnitude.
D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D009602:Nitrosamines / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013464:Sulfuric Acids; D013696:Temperature
D007202:Indicators and Reagents; D009602:Nitrosamines; D013464:Sulfuric Acids