Influence of prednisolone and methotrexate on cellular and biochemical aspects of acute and chronic inflammation studied in subcutaneously implanted sponges in rats.
The effects of prednisolone and methotrexate on the histology and the content of collagen and proteoglycans of the inflammatory tissue in polyurethane sponges impregnated with heat killed M. tuberculosis and implanted subcutaneously in hooded Wistar rats for up to 63 days were studied. Drugs were administered during the 6-day period before sponge removal 7, 21, 35, 49 and 63 days after implantation. Our findings indicate that prednisolone and methotrexate have profound effects on the cellular events of acute and chronic inflammation, and influence the synthesis or degradation of connective tissue macromolecules at certain stages of the inflammatory process.
D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents; D002908:Chronic Disease; D003094:Collagen / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008727:Methotrexate / Q000627:therapeutic use*; D011239:Prednisolone / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D014574:Uronic Acids / Q000032:analysis
D000893:Anti-Inflammatory Agents; D006025:Glycosaminoglycans; D014574:Uronic Acids; D003094:Collagen; D011239:Prednisolone; D006909:Hydroxyproline; D008727:Methotrexate