Synthesis and application of 13C enriched phenylisothiocyanate as a 13C NMR protein probe.
Phenylisothiocyanate, enriched with 13C at the isothiocyanate carbon, has been synthesized and utilized as a 13C NMR probe of proteins for the first time. The reagent has been used to label the amino groups of oxidized glutathione, and the resulting 13C NMR spectrum shows a prominent thiocarbonyl peak after a single NMR scan. The reagent is also capable of differentiating amino groups on the insulin molecule with distinct peaks corresponding to the amino groups on the A and B chains of insulin. This study illustrates the potential of using a new 13C label to functionalize amino groups of proteins and to study the labeled proteins with 13C NMR.
D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D005978:Glutathione; D007328:Insulin; D017879:Isothiocyanates; D009682:Magnetic Resonance Spectroscopy; D011489:Protein Denaturation; D011506:Proteins; D013861:Thiocyanates / Q000138:chemical synthesis*
D002247:Carbon Isotopes; D007328:Insulin; D017879:Isothiocyanates; D011506:Proteins; D013861:Thiocyanates; C005441:phenylisothiocyanate; D005978:Glutathione