Contradistinctive growth responses of cultured rat intestinal epithelial cells to epidermal growth factor depending on cell population density.
The growth response of cultured rat intestinal epithelial (RIE-1) cells to epidermal growth factor (EGF) depends on the cell population density. EGF stimulated the proliferation of RIE-1 cells in dense cultures, but inhibited the proliferation of cells growing at low population densities. In contrast, insulin enhanced RIE-1 cell growth irrespective of the population density. The tumour promoter 12-0-tetradecanoylphorbol 13-acetate (TPA), like EGF, inhibited the proliferation of low-density RIE-1 cells, but differed from EGF in that it did not stimulate the growth of dense cultures.
D000818:Animals; D001769:Blood; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells; D006133:Growth Substances / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007421:Intestine, Small / Q000166:cytology*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D006133:Growth Substances; D007328:Insulin; D004815:Epidermal Growth Factor; D013755:Tetradecanoylphorbol Acetate