title: A locus regulating N-acetylglucosaminidase synthesis during development in dictyostelium.
abstract: The cellular specific activity of N-acetylglucosaminidase increases during development in Dictyostelium discoideum. A monoclonal antibody which specifically recognizes Mr 68,000 and 67,000 forms of N-acetylglucosaminidase was used to show that changes in the relative rate of enzyme synthesis during development parallel the pattern of enzyme accumulation. Developmental and regulatory mutants were isolated to study the relationship between development and enzyme accumulation. No evidence was obtained for any dependence of enzyme accumulation on those genes that are required for aggregation. However, a separate regulatory locus was identified which is involved in enzyme accumulation. Mutations in this gene, nagC, prevent enzyme accumulation during development by preventing an increase in the relative synthetic rate of N-acetylglucosaminidase. The accumulation of other enzymes is unaffected and the mutation causes no developmental defects other than those caused by the loss of N-acetylglucosaminidase activity. The nagC mutation, which is recessive, maps to linkage group VI and is therefore unlinked to the structural gene for N-acetylglucosaminidase.
mesh_terms: D000118:Acetylglucosaminidase / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D000911:Antibodies, Monoclonal; D004023:Dictyostelium / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D004171:Diploidy; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D006596:Hexosaminidases / Q000235:genetics*; D007163:Immunosorbent Techniques; D009154:Mutation
chemical_list: D000911:Antibodies, Monoclonal; D006596:Hexosaminidases; D000118:Acetylglucosaminidase