6 beta-hydroxycortisol in serum and urine as determined by enzyme immunoassay on microtitre plates.
We modified our previous enzyme immunoassay (Clin Chim Acta 1986;157:267-76) for estimating 6 beta-hydroxycortisol (6 beta-OHF) to provide a more routine procedure for measuring it in urine and to increase sensitivity for its measurement in serum. Precision, analytical recovery, specificity, and detection limit are reported. 6 beta-OHF and 17-hydroxycorticosteroids in 24-h and 4-h (08:00-12:00 h) urine collections from healthy volunteers showed no significant day-to-day differences in excretion rate for either collection period. Mean values for 6 beta-OHF in serum of men, women, and women taking oral contraceptives showed no significant differences among these groups.
D015065:17-Hydroxycorticosteroids / Q000652:urine; D000293:Adolescent; D000328:Adult; D002853:Chromatography, Liquid; D003276:Contraceptives, Oral / Q000378:metabolism; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013223:Statistics as Topic
D015065:17-Hydroxycorticosteroids; D003276:Contraceptives, Oral; D007202:Indicators and Reagents; C001380:6 beta-hydroxycortisol; D006854:Hydrocortisone