title: Lipid metabolism indices and plasma corticosterone concentration in chickens treated with prolactin at different time points.
abstract: The effect of exogenous prolactin (PRL) on the weight and lipid content of the liver and abdominal adipose tissue as well as on the concentrations of total lipids and lipoprotein fractions (portomicrons, VLDL, LDL, HDL) in plasma was investigated in chickens. PRL or its solvent were administered at different time points (0, 4, 8, and 12 hours after light onset = HALO) during 5 consecutive days. The birds were exsanguinated at the same time of the day when they received the injections, 24 hours after the last one. At the same time plasma corticosterone (CS) concentration was determined in these chickens and also during 24 hours in intact chickens matched for age. PRL affected fat accumulation in the experimental birds and the intensity of this effect depends on the time of the day at which it was administered. PRL caused rise of the weight of the fat tissue and its lipid content when it was injected at 8 or 12 HALO. This was associated with a significant increase of total plasma lipids and with an insignificant increase in the level of lipoprotein fractions excluding portomicrons. PRL increased also (at 4 HALO) or decreased (at 8 HALO) the liver weight. Handling of the birds during vehicle injections decreased the corticosterone level in the control chickens as compared with the intact ones; PRL raised the level of CS at 0 HALO significantly. The role of PRL in the intensification of fat transport is discussed.
mesh_terms: D000273:Adipose Tissue / Q000032:analysis / Q000033:anatomy & histology / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D002645:Chickens; D003345:Corticosterone / Q000097:blood*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008099:Liver / Q000032:analysis / Q000033:anatomy & histology / Q000187:drug effects; D009929:Organ Size; D011388:Prolactin / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D008055:Lipids; D011388:Prolactin; D003345:Corticosterone