Alterations in cloned Xenopus ribosomal spacers generated by high-frequency plasmid recombination.
In an attempt to understand the mechanisms that have led to the complex intraspecific heterogeneity in spacer organisation found in Xenopus r.DNA, we have transformed Escherichia coli strains that exhibit high levels of plasmid recombination with Xenopus laevis r.DNA sequences inserted in pBR322. We have found that in these recBC sbcA strains, plasmid recombination results in the generation of many types of altered r.DNA spacer at a high frequency. The altered spacers result from two types of deletion/duplication events that occur in the promoter-derived sequences making up much of the spacer. The alterations caused by these events mimic in several ways the different types of organisation found among spacers in the genome. The sbcA-dependent plasmid recombination apparently provides a test system for reconstructing some of the recombinational events that operate during the evolution and maintenance of spacer DNA sequences in vivo.
D000818:Animals; D005075:Biological Evolution; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008954:Models, Biological; D010957:Plasmids; D012335:RNA, Ribosomal / Q000235:genetics; D011995:Recombination, Genetic; D014982:Xenopus laevis / Q000235:genetics*
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal