Induction of class II MHC antigens in vitro on pancreatic B cells isolated from BB/E rats.
The ability of recombinant Interferon-gamma to induce class II expression in vitro on pancreatic islet B cells has been investigated by exposing islets isolated from BB/E and normal Wistar rats to Interferon-gamma and then staining successively with monoclonal antibodies specific for rat class II MHC antigens and insulin. Induction of class II expression was never observed on islet cells obtained from either normal Wistar rats or rats from the BB/E low diabetes incidence (less than 2%) subline. In contrast, pancreatic B cells from rats from the BB/E high diabetes incidence (60-70%) subline expressed class II antigen following culture with Interferon-gamma.
D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000032:analysis*; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051381:Rats; D011919:Rats, Inbred Strains
D006649:Histocompatibility Antigens