Stimulation of human PMNs in vitro by a succinimide molecular complex of methylfurylbutyrolactone.
The immunopotentiating effects on human neutrophils of a new synthetic immune biological response modifer were studied. The compound is a succinimide crystalline molecular complex of methylfurylbutyrolactone (MFBL). The MFBL succinimide (MFBL-S) derivative was tested for its in vitro effects on polymorphonuclear leukocyte (PMN) functions. At microgram quantities, MFBL-S stimulated a twofold increase in: directed migration of PMNs; adherence to nylon; and uptake of E. coli lipopolysaccharide. The MFBL-S enhanced phagocytosis by PMNs of S. epidermidis and E. coli. Additionally, intracellular killing of S. epidermidis by MFBL-S treated PMNs was significantly increased at all doses studied, whereas killing of E. coli was only significantly different from controls at concentrations of 10 micrograms/ml.
D000447:Aldehydes / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D001770:Blood Bactericidal Activity / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D011765:Pyruvaldehyde / Q000494:pharmacology*; D013210:Staphylococcus / Q000276:immunology
D000447:Aldehydes; D008070:Lipopolysaccharides; D011765:Pyruvaldehyde; C046696:nafocare; D001205:Ascorbic Acid