A new method for extracting circular and supercoiled genome segments from cytoplasmic polyhedrosis virus.
The genome of a cytoplasmic polyhedrosis virus (CPV) consisting of 10 segments was extracted by a new two-step extraction method. The results from polyacrylamide gel electrophoresis, phenol and diepoxybutane treatment and electron microscopic examination indicated the extracted genome segments to have circular and supercoiled structures as genome-protein(s) complexes. Multiple cycles of the transcription of each segmented genome of CPV may take place on the circular structure of the segment.
D000818:Animals; D012831:Bombyx; D004591:Electrophoresis, Polyacrylamide Gel; D004852:Epoxy Compounds; D005814:Genes, Viral; D007304:Insect Viruses / Q000235:genetics*; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D010636:Phenols; D012330:RNA, Double-Stranded / Q000302:isolation & purification* / Q000648:ultrastructure; D012367:RNA, Viral / Q000302:isolation & purification* / Q000648:ultrastructure; D014158:Transcription, Genetic
D004852:Epoxy Compounds; D010636:Phenols; D012330:RNA, Double-Stranded; D012367:RNA, Viral; C007366:diepoxybutane