Determination of urokinase in the urine of healthy volunteers and patients with renal diseases.
A sensitive enzyme immunoassay was developed for the determination of urokinase in the plasma and urine. Normal human urine contained 2.68 +/- 0.36 u/ml of UK. Gel filtration of the mixture of normal urine resulted in 2 peaks in the profile, one with molecular weight of 32,000 and the other with molecular weight of 22,000. Patients with glomerulonephritis had higher UK levels. Kidneys with minimal changes and mild type or with the inactive phase of systemic lupus erythematosus (SLE) secreted relatively high levels of UK in the urine.
D000328:Adult; D002850:Chromatography, Gel; D005921:Glomerulonephritis / Q000652:urine; D006801:Humans; D007124:Immunoenzyme Techniques; D007674:Kidney Diseases / Q000652:urine*; D008180:Lupus Erythematosus, Systemic / Q000652:urine; D008297:Male; D008970:Molecular Weight; D014568:Urokinase-Type Plasminogen Activator / Q000652:urine*
D014568:Urokinase-Type Plasminogen Activator