[Mechanism of the inhibitory effect of pranoprofen on sodium urate crystal-induced inflammation].
The mechanism of the inhibitory effect of pranoprofen on sodium urate crystal-induced inflammation was investigated with several inflammatory parameters using leucocytes stimulated with sodium urate crystals in vitro. At 10(-5) M, pranoprofen tended to inhibit the production of chemotactic factor in guinea pig polymorphonuclear leucocytes (PMNL) stimulated with sodium urate crystals and at 10(-4) M, significantly inhibited it. At 10(-3) M and 10(-4) M, it potentiated the chemotaxis of guinea pig PMNL. Furthermore, it inhibited the production of superoxide anion (O2-) in guinea pig PMNL stimulated with sodium urate crystals, with an IC50 value of 5.0 X 10(-4) M, comparable to that of indomethacin. At 10(-3) M, it inhibited the release of beta-glucuronidase stimulated by sodium urate crystals. From doses as low as 10(-6) M, it inhibited dose-dependently the production of PGE2-like substance by the phagocytosis of sodium urate crystals by rat peritoneal leucocytes, with an IC50 value of 7.5 X 10(-6) M. These results suggest that in inhibiting the production of PGE2 stimulated by sodium urate crystals, pranoprofen shows an inhibitory effect on sodium urate crystal-induced inflammation.
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002630:Chemotactic Factors / Q000096:biosynthesis; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D015232:Dinoprostone; D005260:Female; D006074:Gout Suppressants / Q000494:pharmacology*; D006168:Guinea Pigs; D007213:Indomethacin / Q000494:pharmacology; D016209:Interleukin-8; D008297:Male; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D010653:Phenylbutazone / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology*; D011458:Prostaglandins E / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D001578:Benzopyrans; D002630:Chemotactic Factors; D006074:Gout Suppressants; D016209:Interleukin-8; D011422:Propionates; D011458:Prostaglandins E; C043940:pyranoprofen; D010653:Phenylbutazone; D015232:Dinoprostone; D003078:Colchicine; D007213:Indomethacin