title: The splenic microenvironment and self recognition as factors in allograft rejection in rats. A study using indium-111-labeled cells.
abstract: Splenectomy facilitates organ allograft survival in some rat strains, and in weak donor-recipient histoincompatible pairs. We have found using a heart spleen "twin" graft model, using ACI rats as recipients and Lewis rats as donors, that the transplanted heart will survive in most recipients after delayed host splenectomy. The presence of a viable mass of splenic tissue will allow rejection to proceed only when the transplanted spleen is of host origin, and not when it comes from the donor (i.e., when it is allogeneic). The use of 111In-labeled cells has allowed us to show that lymphocyte traffic and trapping is markedly altered in the transplanted allogeneic spleens, when compared with control transplanted syngeneic spleens. Thus, despite the presence of the splenic "microenvironment," cardiac allograft rejection does not occur in the absence of syngeneic splenic tissue. We conclude that the role of the spleen in the immune response is to facilitate the recognition of self and the acquisition of alloreactivity in weak responder rat strains and donor-recipient pairs.
mesh_terms: D000818:Animals; D006084:Graft Rejection; D016027:Heart Transplantation; D007204:Indium; D011868:Radioisotopes; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology* / Q000637:transplantation; D013156:Splenectomy; D014184:Transplantation, Homologous
chemical_list: D011868:Radioisotopes; D007204:Indium