[Genome organization of the plasmid of IncQ/P4 group and its vector derivatives].
The genome organization and functioning of IncQ/P4 plasmids are reviewed. Based on these plasmids, cloning vectors have been constructed for broad host range of gram-negative bacteria. Together with one- and two-replicon vectors for cloning via insertion inactivation of markers, specialized plasmid vectors are described: cosmids, promoter-probe vectors, vectors for direct selection of recombinant molecules. Examples of using broad host range vectors for gene cloning and expression in non-enteric gram-negative bacteria are presented.
D003001:Cloning, Molecular; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D010957:Plasmids
Organizatsiia genoma plazmid IncQ/P4-gruppu i vektory, skonstruirovannye na ikh osnove.