Inhibition of E. coli adenylate cyclase activity by inorganic orthophosphate is dependent on IIIglc of the phosphoenolpyruvate:glycose phosphotransferase system.
The relationship of adenylate cyclase, inorganic orthophosphate and the proteins of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) was studied. A strain deleted for the genes for Enzyme I and IIIglc of the PTS was transformed with plasmids expressing either Enzyme I and HPr, IIIglc or all three proteins. The fully reconstituted strain showed a Pi-dependent stimulation of adenylate cyclase activity; in contrast, the strain expressing only IIIglc showed a Pi-dependent inhibition of adenylate cyclase activity.
D000067956:Adenylyl Cyclase Inhibitors; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D009154:Mutation; D010710:Phosphates / Q000494:pharmacology*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000502:physiology*; D010750:Phosphoproteins / Q000235:genetics / Q000502:physiology*; D010957:Plasmids; D014169:Transformation, Bacterial
D000067956:Adenylyl Cyclase Inhibitors; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D010710:Phosphates; D010750:Phosphoproteins; C032242:crr protein, E coli; C033623:factor III(Glc); D010731:Phosphoenolpyruvate Sugar Phosphotransferase System