title: Influence of lipid environment on insulin binding in cultured hepatoma cells.
abstract: The influence of alterations of plasma membrane physico-chemical properties on insulin binding have been characterized in an insulin-sensitive rat hepatoma cell line adapted to grow for several generations in culture medium enriched with linoleic acid (18:2) or with 25-hydroxycholesterol. The cells took up 18:2 and 25-hydroxycholesterol added to the culture medium, without exhibiting any sign of intolerance or intoxication. These compounds respectively increased and decreased membrane fluidity at 37 degrees C. The cells demonstrated extensive changes in insulin binding parameters in response to experimental modifications of their membrane lipid composition. When determined at 4 degrees C, insulin receptors were present in the control cells at 136,000 sites/cell but this fell to 111,000 (P less than 0.05) in cells enriched in 18:2, and rose to 176,000 (P less than 0.001) in hydroxysterol-grown cells. According to a two-site model, the main effect of 18:2 was a significant increase of the number of high-affinity sites with a concomitant decrease of low-affinity sites. The hydroxysterol had the opposite effects on these parameters. The high-affinity insulin binding capacity of the hepatoma cells was affected by lipid supplementation in a similar way, whether it was determined at 4 degrees C or at 37 degrees C. Assuming a negative cooperativity model, 18:2 enhanced the degree of negative cooperativity among the sites, while 25-hydroxycholesterol reduced it. The time-course of insulin-induced receptor down-regulation was accelerated in the cells enriched in polyunsaturated fatty acids, but reduced in cells exposed to 25-hydroxycholesterol. These insulin-binding alterations cannot be directly related to modifications of cellular growth rate, receptor internalization or membrane fluidity per se, and are discussed as being more likely due to membrane lipid composition than to overall cell metabolism modifications.
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D006888:Hydroxycholesterols / Q000502:physiology; D007328:Insulin / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000502:physiology; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000502:physiology; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism*
chemical_list: D006888:Hydroxycholesterols; D007328:Insulin; D008041:Linoleic Acids; D008563:Membrane Lipids; C007997:25-hydroxycholesterol; D019787:Linoleic Acid; D011972:Receptor, Insulin