An enzyme-immunoassay for human interleukin-2.
A highly sensitive enzyme-immunoassay (EIA) for human interleukin-2 (IL-2) has been established. The assay is based on a sandwich method that uses two kinds of anti-IL-2 antibodies raised against Escherichia coli-derived recombinant IL-2 (rIL-2). An affinity-purified-anti-IL-2 goat IgG was used as the first antibody and the Fab' fragment of an affinity-purified-anti-IL-2 rabbit IgG was used as the second antibody after being coupled with horseradish peroxidase (HRP). As little as 30 pg/ml of IL-2 was detected by the EIA, indicating that this method was about 100 times more sensitive than the bioassay using an IL-2-dependent murine natural killer cell line, NKC3. There was a good correlation between the EIA and the bioassay (r = 0.998).
D001681:Biological Assay; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007376:Interleukin-2 / Q000032:analysis*
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007376:Interleukin-2; D006735:Horseradish Peroxidase