title: Expression of myosin heavy chain isoforms in regenerating myotubes of innervated and denervated chicken pectoral muscle.
abstract: Monoclonal antibodies were prepared to stage-specific chicken pectoral muscle myosin heavy chain isoforms. From comparison of serial sections reacted with these antibodies, the myosin heavy chain isoform composition of individual myofibers was determined in denervated pectoral muscle and in regenerating myotubes that developed following cold injury of normal and denervated muscle. It was found that the neonatal myosin heavy chain reappeared in most myofibers following denervation of the pectoral muscle. Regenerating myotubes in both innervated and denervated muscle expressed all of the myosin heavy chain isoforms which have thus far been characterized in developing pectoral muscle. However, the neonatal and adult myosin heavy chains appeared more rapidly in regenerating myotubes compared to myofibers in developing muscle. While the initial expression of these isoforms in the regenerating areas was similar in innervated and denervated muscles, the neonatal myosin heavy chain did not disappear from noninnervated regenerating fibers. These results indicate that innervation is not required for the appearance of fast myosin heavy chain isoforms, but that the nerve plays some role in the repression of the neonatal myosin heavy chain.
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002645:Chickens; D005260:Female; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009121:Muscle Denervation; D009132:Muscles / Q000294:innervation / Q000502:physiology*; D009218:Myosins / Q000276:immunology / Q000378:metabolism*; D012038:Regeneration
chemical_list: D000911:Antibodies, Monoclonal; D009218:Myosins