Human allospecific cytolytic T lymphocyte lysis of a murine cell transfected with HLA-A2.
A variety of molecules are involved in the interaction of human allospecific cytolytic T lymphocytes (CTL) with target cells. Monoclonal antibodies specific for these molecules inhibit CTL-target conjugate formation and/or lysis. To further study recognition and lysis of targets by human CTL, we used a murine mastocytoma cell line transfected with the histocompatibility leukocyte antigen (HLA)-A2 gene (P815-A2+) as a target for human HLA-A2-specific CTL. We find that only a subset of human HLA-A2-specific CTL can lyse murine P815-A2+ cells, suggesting that the murine cells may lack one or more accessory molecules needed for CTL recognition and lysis.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D015789:HLA-A2 Antigen; D006801:Humans; D007111:Immunity, Cellular; D016169:Lymphocyte Function-Associated Antigen-1; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006680:HLA Antigens; D015789:HLA-A2 Antigen; D016169:Lymphocyte Function-Associated Antigen-1