Demonstration of Skn antigens on mouse epidermal cells by immunofluorescence and flow cytometry.
C57BL/6 (B6) mice that were lethally irradiated and reconstituted with (B6XA/J)F1 spleen cells were immunized against the skin-specific antigens, Skn, by grafting with A/J tail skin. Serum from these mice was shown to contain Skna-specific antibody by a flow cytometric assay using indirect immunofluorescence.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis*; D002678:Chimera; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D012867:Skin / Q000166:cytology / Q000276:immunology*
D000954:Antigens, Surface; D001324:Autoantigens