Preparative reversed-phase high-performance liquid chromatography of iodinated insulin retaining full biological activity.
Insulin monoiodinated in Tyr A14, A19, B16 and B26 can be separated from insulin and diiodoinsulins using reversed-phase high-performance liquid chromatography on LiChrosorb RP-18 columns. Monoiodoinsulins with high and low specific activities were isolated from a number of buffer systems without any reduction in binding affinity and biological activity in isolated rat fat cells. The reason for the previously observed reduction in the binding affinity was probably column bleeding, i.e., chemical degradation of the column support.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000378:metabolism; D007202:Indicators and Reagents; D007328:Insulin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D012825:Silicon; D013552:Swine
D007202:Indicators and Reagents; D007328:Insulin; C008147:insulin, iodo-; D005947:Glucose; D012825:Silicon