Immunocytochemical demonstration of glial fibrillary acidic protein in the cortical perivascular glial sheat of the cat.
The peroxidase-antiperoxidase method was used on paraffin embedded material to demonstrate the distribution of the glial fibrillary acidic protein (GFA protein) in the perivascular glial membrane of the cortical blood vessels of cats. The nature of the dense zones was determined by ultrastructural immunocytochemistry with anti-GFA protein. Immunoreactivity was seen exclusively in the dense zones of the perivascular glia and in the astrocytic processes of the neuropil. The dense zones may exert a stabilizing function on the basal membrane during the changes in the caliber of the vessels.
D000818:Animals; D002415:Cats; D002540:Cerebral Cortex / Q000098:blood supply* / Q000648:ultrastructure; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009457:Neuroglia / Q000032:analysis*
D005904:Glial Fibrillary Acidic Protein