Acute inflammation in gram-negative infection: endotoxin, interleukin 1, tumor necrosis factor, and neutrophils.
Experimental bacterial infection of the dermis induced with gram-negative microorganisms is associated with an acute inflammatory reaction, which represents the principal local defense against spread of the infection. When the inflammatory reaction is quantitated with radiolabeled cells and proteins, the kinetics resemble acute inflammation induced with other agents, such as immune complexes or chemotaxins. There is an interrelationship between the components or events of the inflammatory reaction; inasmuch as vascular injury is neutrophil-dependent, neutrophils must migrate to the site where the bacteria multiply. In neutropenic animals there is no such emigration and bacterial multiplication is not inhibited. The microorganisms shed endotoxin, which in turn induces secretion of interleukin 1 (IL 1) and probably tumor necrosis factor. Endotoxin is the most potent agent (10(-15) mol vs. 10(-12) mol of C5ades Arg) capable of inducing a neutrophil influx. Desensitization or tachyphylaxis of the tissues (probably of postcapillary venular endothelium) to IL 1 seems to control cessation of the neutrophil influx (also in vitro evidence). Phagocytosis of the bacteria by neutrophils is associated with release of oxygen radicals and lysosomal proteases from the neutrophils. These are instrumental in eliciting microvascular injury, which is characterized by enhanced vasopermeability, hemorrhage, and thrombosis.
D000208:Acute Disease; D000818:Animals; D003165:Complement System Proteins / Q000276:immunology; D004731:Endotoxins / Q000633:toxicity; D004927:Escherichia coli Infections / Q000473:pathology*; D006023:Glycoproteins / Q000502:physiology; D007249:Inflammation / Q000209:etiology / Q000503:physiopathology*; D007375:Interleukin-1 / Q000502:physiology; D007970:Leukopenia / Q000503:physiopathology; D008833:Microcirculation; D009504:Neutrophils / Q000502:physiology; D011817:Rabbits; D012874:Skin Diseases, Infectious / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha
D004731:Endotoxins; D006023:Glycoproteins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D003165:Complement System Proteins