Repression of meiotic crossing over by a centromere (CEN3) in Saccharomyces cerevisiae.
The location of the centromere of chromosome III (CEN3) of Saccharomyces cerevisiae has been altered by means of transformation. The frequency of meiotic crossing over in the CEN3-PGK1 and LEU2-CEN3 intervals increases approximately 1.5- and fourfold, respectively, when CEN3 is repositioned at HIS4. The centromere-distal HIS4-LEU2 region experiences a three- to fivefold decrease in the frequency of meiotic exchange when CEN3 is repositioned at HIS4. The inhibition of meiotic crossing over is conferred by a 627-base-pair fragment of CEN3 DNA and is not dependent on the orientation of CEN3 relative to the rest of chromosome III.
D002503:Centromere / Q000502:physiology*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000502:physiology*; D003434:Crossing Over, Genetic; D005838:Genotype; D008540:Meiosis; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D013489:Suppression, Genetic