Use of antibodies to probe membrane glycoproteins associated with drug-resistant J774.2 cells.
Three drug-resistant sublines of the murine macrophage-like cell line J774.2 were selected in vitro for their ability to grow in high concentrations of either taxol, vinblastine, or colchicine. Each contains a major plasma membrane glycoprotein (130-150 kDa), which is barely seen in the drug-sensitive parental cell line. Polyclonal antibodies, raised against the glycoproteins present in the colchicine- and vinblastine-resistant cells, were used to probe for relationships among the three glycoproteins. Our observations suggest that the glycoproteins from the different drug-resistant cell lines share many common domains but are not identical.
D000818:Animals; D000918:Antibody Specificity; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D004351:Drug Resistance; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology*; D008264:Macrophages; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis
D006023:Glycoproteins; D008565:Membrane Proteins; D010446:Peptide Fragments