Expression of an interferon-alpha gene variant in E. coli using tandemly repeated synthetic ribosomal binding sites.
A human interferon-alpha 2 gene variant was expressed in Escherichia coli under the control of the tryptophan operon promoter and of a series of synthetic partially overlapping ribosomal binding sites, which control initiation of translation. Variation in the distance between the start codon and such a set of ribosomal binding sites affected the level of interferon expression much less than previously described for single ribosomal binding sites.
D001483:Base Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005809:Genes, Regulator; D007370:Interferon Type I / Q000235:genetics*; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000235:genetics
D004274:DNA, Recombinant; D007370:Interferon Type I; D014364:Tryptophan