Inhibitory effects of beta 2-microglobulin on in vitro calcification of osteoblastic cells.
Elevated levels of aluminum and beta 2-microglobulin have been demonstrated in chronic dialysis patients. The role of aluminum in the pathogenesis of renal osteodystrophy has also been shown. We report on the effects of beta 2-microglobulin on calcification in vitro using osteoblastic cells, clone MC3T3-El. At concentrations comparable to those in plasma of chronic dialysis patients, both beta 2-microglobulin and aluminum suppressed calcification while collagen synthesis and alkaline phosphatase activity were maintained. These observations may be related to the impaired bone mineralization frequently observed in chronic dialysis patients.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000535:Aluminum / Q000494:pharmacology; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002999:Clone Cells; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000494:pharmacology*
D001613:beta 2-Microglobulin; D000535:Aluminum; D000469:Alkaline Phosphatase; D002118:Calcium