Detection of tetanus toxoid antibodies in human sera in New Zealand by ELISA.
An enzyme-linked immunosorbent assay (ELISA) incorporating the sensitive biotin-streptavidin system was developed to detect IgG antibodies to tetanus toxoid in human serum. Serum samples obtained from 557 normal persons aged 1-65 years from different areas in New Zealand were tested. The proportion of those immune ranged from 60-93% in males, and from 46-86% in females. In the 1-9 years age group 85% were immune. The indirect ELISA is suitable for serological surveys as it is simple to perform, economical and reproducible.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009520:New Zealand; D013742:Tetanus / Q000453:epidemiology; D013745:Tetanus Toxoid / Q000276:immunology*
D000907:Antibodies, Bacterial; D013745:Tetanus Toxoid