Anti-immunoglobulin antibody detection in adjuvant arthritis by an ELISA technique.
Sprague-Dawley rats, injected in the hind paw with heat-killed mycobacteria dispersed in oil, develop a severe polyarthritis. In this paper, we detected and quantified by a micro-ELISA technique autoantibodies against immunoglobulins in rats with adjuvant arthritis. Increased total anti-immunoglobulin antibodies levels were found from 3 days after induction and remained elevated until day 42. IgG anti-immunoglobulin antibodies in arthritic animals were significantly elevated during days 35-42. These results show that alterations in the humoral immune response (synthesis of anti-immunoglobulin antibodies) are present in adjuvant arthritis as they are in human rheumatoid arthritis.
D000818:Animals; D001168:Arthritis / Q000276:immunology*; D001169:Arthritis, Experimental / Q000209:etiology / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051381:Rats; D011919:Rats, Inbred Strains; D012217:Rheumatoid Factor / Q000032:analysis*; D013997:Time Factors
D012217:Rheumatoid Factor