Evaluation of the Cellmatics and direct monoclonal fluorescence antibody staining for detection of genital chlamydial infections.
Three methods for Chlamydia trachomatis detection were compared: the Cellmatics (Difco Laboratories, Detroit, MI), a commercially available tissue culture system which contains a rat cell line; a direct fluorescent Chlamydia antibody (DFA) (Microtrak, Syva Corp., Palo Alto, California) stain; and a standard tissue culture isolation method employing McCoy cells. Of the 121 specimens in the study, 20 were positive by the standard cell culture. All 20 of these specimens were also positive by the Cellmatics but only 10 were positive by DFA.
D002460:Cell Line; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D005831:Genital Diseases, Female / Q000175:diagnosis*; D005832:Genital Diseases, Male / Q000175:diagnosis*; D006801:Humans; D008297:Male; D014521:Urethra / Q000382:microbiology