An enzyme immunoassay for mouse epidermal growth factor utilizing a liquid phase double-antibody system.
An enzyme immunoassay for mouse epidermal growth factor (EGF) involving a liquid phase double-antibody system was developed. The EGF-beta-galactosidase conjugate prepared was stable for at least 8 months. By this method, EGF was detectable at a concentration as low as 20 pg per tube. The concentrations of EGF in various tissues of mice are also presented.
D000818:Animals; D004815:Epidermal Growth Factor / Q000032:analysis*; D005260:Female; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012737:Sex Factors; D013363:Submandibular Gland / Q000032:analysis; D014018:Tissue Distribution
D004815:Epidermal Growth Factor