title: Characterization of the regulatory region of the Escherichia coli penicillin acylase structural gene.
abstract: Penicillin acylase is utilized in the enzymatic production of semisynthetic penicillins. The enzyme is composed of two different subunits that originate from a common precursor. The partial nucleotide (nt) sequence of the structural gene has been published. This paper reports the nt sequence of the regulatory region of this gene, the identification of a functional promoter, the transcriptional start point, and the description of possible regulatory regions.
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D010399:Penicillin Amidase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011953:Receptors, Cyclic AMP / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D011953:Receptors, Cyclic AMP; D000581:Amidohydrolases; D010399:Penicillin Amidase