Analysis of the cell membrane proteolytic enzymes of the B16, F1, F10, and BL6 melanoma and their role in target cell destruction.
The tumor-induced red blood cell (RBC) cytolysis assay has been used to demonstrate that three B16 melanoma sublines, the F1, F10, and BL6, cause the cytolysis of normal red blood cells in vitro. RBC cytolysis was inhibited for all three sublines by metalloprotease inhibitors. Cell membrane preparations have been prepared for all three sublines and tumor cell membrane-induced RBC cytolysis was also shown to be inhibited by metalloprotease inhibitors. The F10 and BL6 sublines were shown to have cell membrane-bound proteases. The BL6 subline has a cell membrane enriched in an enzyme with a trypsin-like arginine specificity. The trypsin-like protease may have a metal dependence. The BL6 subline has a collagenolytic cell membrane enzymes and a chymotrypsin-like cell membrane enzyme. B16 cell membrane enzymes may be responsible for RBC cytolysis in vitro in a process requiring divalent cations.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D003094:Collagen; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005342:Fibrinolysis; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D008545:Melanoma / Q000201:enzymology* / Q000473:pathology; D008670:Metals; D051379:Mice; D008815:Mice, Inbred Strains; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010447:Peptide Hydrolases / Q000032:analysis*; D011480:Protease Inhibitors / Q000494:pharmacology
D008670:Metals; D011480:Protease Inhibitors; D003094:Collagen; D010447:Peptide Hydrolases