Degradation of dipalmitoyl phosphatidylcholine by isolated rat granular pneumocytes and reutilization for surfactant synthesis.
We investigated metabolic utilization of exogenous (modelled after lung surfactant) phospholipids by granular pneumocytes in primary culture. Cells were incubated for 21, 65, and 140 min with [3H-methyl]dipalmitoylphosphatidylcholine (DPPC) containing liposomes prepared from synthetic lipids. Radioactivity in cellular phosphatidylcholine (PC) declined steadily to 50% of the total trypsin-resistant cell-associated radioactivity. The proportion of radioactivity increased with time in cytidine-5'-diphosphate-choline and phosphorylcholine, which suggested reutilization of choline for PC synthesis. Cells incubated with liposomes for 2 h revealed that of the total cell-associated radioactivity, 7% was in lamellar bodies and 10% in the microsomal fraction. The lipid-associated radioactivity was 24% in "soluble," 96% in lamellar bodies, and 92% in the microsomal fraction. Percent of total PC label recovered in disaturated PC of microsomal fractions decreased (slope = -5.27%/h) with time of incubation (r = 0.67). Incubation of cells with liposomes containing ([3H-methyl]choline-[14C]palmitoyl) DPPC led to altered isotope ratios in both lamellar bodies and microsomes. These observations indicate that granular pneumocytes degrade exogenous PC and resynthesize PC from degradation products.
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism*; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002738:Chloroquine / Q000494:pharmacology; D002794:Choline / Q000378:metabolism; D008081:Liposomes; D008168:Lung / Q000166:cytology* / Q000378:metabolism; D008744:Methylamines / Q000494:pharmacology; D009940:Organoids / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000096:biosynthesis*; D011796:Quinacrine / Q000494:pharmacology; D051381:Rats
D008081:Liposomes; D008744:Methylamines; D011663:Pulmonary Surfactants; D000643:Ammonium Chloride; D015060:1,2-Dipalmitoylphosphatidylcholine; D002738:Chloroquine; C027451:methylamine; D011796:Quinacrine; D002794:Choline