[Clonogenic capacity and proliferative activity of the cells of malignant tumors of the bones].
The authors presented their first results of tumor cell cloning in 48 patients with malignant bone tumors. Colony growth was observed in 32 patients. Colony formation efficacy, expressed in the number of grown colonies per 10(5) tumor cells incubated in the culture, varied greatly (from 0.4 to 90.7) in different patients. Rather a high colony forming capacity of tumor cells was prognostically unfavorable. Tumor cells in the patients with malignant bone tumors were characterized by a low proliferative activity: the ratio of DNA-synthesizing cells evaluated by immunofluorescence or flow cytometry in 14 examinees was an average of 3.3% varying in different patients from 0 to 8.0%. No distinct correlation between tumor cell colony forming capacity and proliferative activity was revealed in the patients.
D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D002648:Child; D002675:Child, Preschool; D002999:Clone Cells; D003470:Culture Media; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D012512:Sarcoma, Ewing / Q000378:metabolism / Q000473:pathology*
D003470:Culture Media; D004273:DNA, Neoplasm
Klonogennaia sposobnost' i proliferativnaia aktivnost' kletok zlokachestvennykh opukholeĭ kosteĭ.