Angiogenic activity in human wound fluid.
Wound drainage fluid from postoperative patients was examined for its ability to induce blood vessel growth using the chick chorio-allantoic membrane (CAM) assay. Sixteen samples from 12 patients were tested. The tests were performed on both whole unfractionated fluid and fluid after separation using diethyl aminoethyl cellulose (DEAE) ion exchange chromatography. All 16 samples were inactive before DEAE-chromatography but a fraction, eluting at between 300-400 mM salt, induced neovascularization in 15 out of 16 samples. These differences were statistically highly significant (p less than 0.001). Angiogenic factor (AF) was detected in fluids obtained from both clean and contaminated wounds, and in patients with benign and malignant disease. As mentioned earlier unfractionated wound fluids were nonangiogenic by themselves and their addition to the active fractions greatly reduced the angiogenic effect of the latter. Therefore we suggest that wound fluid contains both promoters and inhibitors of angiogenesis. The local balance between these may govern the rate of vascularisation during healing.
D043925:Angiogenesis Inducing Agents / Q000032:analysis*; D001681:Biological Assay; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D009369:Neoplasms / Q000601:surgery; D013514:Surgical Procedures, Operative; D014945:Wound Healing; D014950:Wounds, Penetrating / Q000378:metabolism*
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances