title: Alterations to the microtubular cytoskeleton and increased disorder of chromosome alignment in spontaneously ovulated mouse oocytes aged in vivo: an immunofluorescence study.
abstract: Alterations in the organization of the microtubular cytoskeleton and chromosome alignment were examined by tubulin immunofluorescence and DAPI staining during in vivo ageing of naturally ovulated, metaphase-arrested oocytes of CBA/Ca mice in the fallopian tubes. In oocytes isolated from young mice on the day of oestrus, a few hours after ovulation, when they are still tightly surrounded by cumulus, the anti-tubulin fluorescence is almost exclusively restricted to the metaphase spindle. Only some faintly staining foci are observed in the cytoplasm, which presumably represent cytoplasmic MTOC not involved in spindle formation. The spindle is usually barrel-shaped or slightly pointed at its poles and does not possess astral fibres. In oocytes aged for more than 12 h in the fallopian tubes cytoplasmic asters develop, while microtubules seem to become gradually lost from the spindle, preferentially in its central area near the chromosomes. Astral fibres are observed radiating out from the polar centrosomes into the cytoplasm. In oocytes free of cumulus, and consequently more than 24 h post-ovulation, a pronounced shrinking of the spindle is observed. The mean pole-to-pole distance becomes significantly reduced in postovulatory aged cells. At the same time astral microtubules in the cytoplasm appear to become gradually depolymerized. Age-dependent alterations in the microtubular cytoskeleton do not seem to result from a changed pattern of the post-translational detyrosylation of alpha-tubulin in certain sets of microtubules. In freshly ovulated oocytes chromosomes in most spindles are well ordered and precisely arranged at the equatorial plane.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002470:Cell Survival; D002875:Chromosomes / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D005260:Female; D005455:Fluorescent Antibody Technique; D008677:Metaphase; D051379:Mice; D008808:Mice, Inbred CBA; D008870:Microtubules / Q000648:ultrastructure*; D009630:Nondisjunction, Genetic; D009865:Oocytes / Q000166:cytology* / Q000648:ultrastructure; D010060:Ovulation; D008941:Spindle Apparatus / Q000648:ultrastructure*; D014404:Tubulin / Q000032:analysis
chemical_list: D014404:Tubulin