Solid phase enzyme immunoassays of pertussis toxin using peroxidase or biotin labelled antibodies.
The quantitative determination of pertussis toxin (PT) is generally estimated by biological tests which are time-consuming, cumbersome and unsuitable for simultaneous testing of a large number of samples. The present work describes a rapid and sensitive ELISA procedure allowing PT assay based on a sandwich technique amplified via avidin-biotin interaction. As low as 0.1 ng of PT in 0.1 ml sample could be detected by the procedure described.
D001360:Avidin; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase; D007700:Kinetics; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000032:analysis*
D010566:Virulence Factors, Bordetella; D001360:Avidin; D001710:Biotin; D006735:Horseradish Peroxidase; D037342:Pertussis Toxin