Separation of amino acids and antibiotics by narrow-bore and normal-bore high-performance liquid chromatography with pre-column derivatization.
The selectivity, efficiency and lifetime of normal- and narrow-bore columns for high-performance liquid chromatography were investigated for the separation and quantification of amino acids and the amino acid-like antibiotics phosphinothricin and phosphinothricylalanylalanine in biological samples. These compounds were determined by an automated pre-column derivatization with o-phthalaldehyde-2-mercaptoethanol reagent and UV detection at 338 nm.
D000596:Amino Acids / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D008623:Mercaptoethanol; D013056:Spectrophotometry, Ultraviolet; D009764:o-Phthalaldehyde
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde