Enzyme-linked immunolysis assay for tumour-specific surface antigens.
Expression of the activity of the cytosolic enzyme lactate dehydrogenase released on cell lysis by tumour specific antibodies in the presence of complement was investigated by direct assay of the expression of the occluded enzyme in tumour cell suspensions. This method, called Enzyme Linked Immunolysis Assay (ELILA) was shown to be more sensitive than conventional cytotoxic assays. The data fit well in the linear regression model, so that the technique can be used for quantitation of the antibody titres.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000920:Antibody-Dependent Cell Cytotoxicity; D000951:Antigens, Neoplasm / Q000032:analysis*; D003167:Complement Activation; D003601:Cytotoxicity Tests, Immunologic; D007124:Immunoenzyme Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D009374:Neoplasms, Experimental / Q000276:immunology; D051381:Rats
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007770:L-Lactate Dehydrogenase