Thrombogenicity of a fibronectin-coated, experimental polytetrafluoroethylene graft.
To determine if pretreatment of polytetrafluoroethylene (PTFE) vascular grafts with fibronectin (FN) increased platelet adherence to them and adversely affected their patency, we labeled autologous canine platelets with indium III-oxine and interposed a FN-coated, experimental, 4 mm inner diameter, 10 cm long PTFE prosthesis into one carotid artery of 10 dogs. An identical graft, lacking only the FN coating, was implanted as a control in the contralateral carotid artery. A second group of 10 dogs was similarly treated, but each animal received 2 grains of aspirin and 50 mg of dipyridamole (Persantine) daily, beginning 3 days before surgery and continuing for the duration of the experiment. By means of a quantitative, gamma-camera, platelet adherence to the grafts was studied for 5 days after implantation. Graft patency was assessed with the Doppler velocity meter and was confirmed by surgical reexploration when thrombosis was suspected. FN coating increased platelet adhesion to the grafts in animals untreated with aspirin by a factor of threefold to fivefold. In those animals receiving antiplatelet drugs, patency of FN-coated grafts at 5 days was significantly (p less than 0.05) higher (80%) than in untreated animals (27.2%). In addition, mean platelet deposition on the grafts in these animals was reduced compared with untreated controls. Thus although FN coating of PTFE grafts significantly increases their affinity for platelets, this effect can be effectively abolished by pretreatment with aspirin and dipyridamole.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001807:Blood Vessel Prosthesis; D004176:Dipyridamole / Q000494:pharmacology; D004285:Dogs; D005353:Fibronectins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011138:Polytetrafluoroethylene; D013927:Thrombosis / Q000209:etiology*; D014654:Vascular Patency / Q000187:drug effects*
D005353:Fibronectins; D004176:Dipyridamole; D011138:Polytetrafluoroethylene; D001241:Aspirin