Iris-ciliary complex inhibits protein synthesis by organ cultured mammalian ocular lens cells.
Freshly isolated rabbit ocular lenses with or without adhering iris-ciliary complex were organ cultured. It was found that lenses with iris-ciliary complex showed decreased protein synthetic ability. Vimentin, a cytoskeletal protein and MP-26, a membrane associated protein, were used as markers for protein synthetic activity. Removal of the iris-ciliary complex at least partially restores the protein synthetic capability of the lens. The ocular lenses cultured with iris-ciliary complex for 48 hr showed marked equatorial opacities.
D000818:Animals; D020346:Aquaporins; D002924:Ciliary Body / Q000502:physiology*; D005136:Eye Proteins / Q000096:biosynthesis*; D007498:Iris / Q000502:physiology*; D007908:Lens, Crystalline / Q000378:metabolism*; D008562:Membrane Glycoproteins; D009924:Organ Culture Techniques; D011817:Rabbits; D014746:Vimentin / Q000096:biosynthesis*
D020346:Aquaporins; D005136:Eye Proteins; D008562:Membrane Glycoproteins; D014746:Vimentin; C030328:aquaporin 0