[A stepwise method of evaluating sugar substitutes--a preliminary study using enzymes. 1. Alpha-glucosidase from yeast].
alpha-Glucosidase from yeast was checked for its catalytic potency under a variety of experimental conditions. Michaelis constants and maximal velocities are reported for 7 disaccharides of the glucosyl-fructosyl or glucosyl-glucosyl type, 4 disaccharide alcohols, and 2 mixtures of each 2 disaccharide alcohols. Reduction of a carbonyl group is of less importance for the substrate properties than the type of the glycoside bond; consequences for the suitability of potential sugar substitutes are derived.
D004187:Disaccharides / Q000378:metabolism; D006868:Hydrolysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013549:Sweetening Agents / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000378:metabolism*
D004187:Disaccharides; D013549:Sweetening Agents; D000520:alpha-Glucosidases
Stufenweises Prüfschema für Zuckeraustauschstoffe--Vorprüfung mittels Enzymen. 1. alpha-Glucosidase aus Hefe.