Implementation of a semiclosed large scale counterflow centrifugal elutriation system.
A semiclosed counterflow centrifugal elutriation system that minimizes the risks of contamination and cell loss is presented. A detailed description of the configuration of the system and its sterilization and assembly is provided along with examples of its application to large-scale separation of leukapheresis buffy coat and cadaveric bone marrow. We are currently using this system to deplete lymphocytes from human bone marrow prior to use in allogeneic bone marrow transplantation. The implementation of a semiclosed system increases the safety, ease of operation, and reproducibility of a technique that has the potential for a wide range of clinical applications.
D002469:Cell Separation / Q000295:instrumentation*; D002498:Centrifugation / Q000295:instrumentation; D003377:Countercurrent Distribution; D006801:Humans