[Staphylococcal toxic shock exotoxin (its isolation and characteristics)].
The method for obtaining the preparation of toxic shock exotoxin (TSE) has been developed. This method comprises the following operations: the sorption of the toxin from the culture fluid on Amberlite CG-50, elution, dialysis, gel chromatography in a column with biogel P-2, isoelectric focusing, and gel chromatography in a column with Sephadex G-75. TSE is a relatively thermostable protein with a molecular weight of 24,000. Its isoelectric point is 7.2. Monospecific antiserum to TSE with precipitating antibody titer equal to 1:16, identical to the reference serum (M. S. Bergdoll), has been prepared. This antiserum has shown no cross reactions with the homogeneous preparations of staphylococcal enterotoxins.
D000818:Animals; D001431:Bacteriological Techniques; D003429:Cross Reactions; D005098:Exotoxins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D011817:Rabbits; D012772:Shock, Septic / Q000382:microbiology*; D013211:Staphylococcus aureus
D005098:Exotoxins; D007106:Immune Sera
Stafilokokkovyĭ ékzotoksin toksicheskogo shoka (vydelenie i kharakteristika).