Cloning, expression and sequencing the molybdenum-pterin binding protein (mop) gene of Clostridium pasteurianum in Escherichia coli.
mop is the structural gene for the molybdenum-pterin binding protein, which is the major molybdenum binding protein in Clostridium pastuerianum. The mop gene was detected by immunoscreening genomic libraries of C. pastuerianum and identified by determining the nucleotide sequence of the cloned insert of clostridial DNA. The deduced amino acid sequence of an open reading frame proved to be identical to the first twelve residues of purified Mop. The DNA sequence flanking the mop gene contains promoter-like consensus sequences which are probably responsible for the expression of Mop in Escherichia coli. The deduced amino acid composition shows that the protein is hydrophobic, lacks aromatic and cysteine residues and has a calculated molecular weight of 7,038. The N-terminal amino acid sequence of Mop has sequence homology with DNA binding proteins. The pattern and type of residues in the N-terminal region suggest it forms the helix-turn-helix structure observed in DNA binding proteins. We propose that Mop may be a regulatory protein binding the anabolic source of molybdenum.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics*; D003067:Coenzymes; D004269:DNA, Bacterial / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008667:Metalloproteins; D008982:Molybdenum / Q000378:metabolism*; D000090006:Molybdenum Cofactors; D011621:Pteridines / Q000378:metabolism*; D011622:Pterins
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003067:Coenzymes; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D008667:Metalloproteins; D008982:Molybdenum; D000090006:Molybdenum Cofactors; D011621:Pteridines; D011622:Pterins; C050771:mopI protein, Clostridium pasteurianum; C022261:molybdopterin