title: Thymocyte-dependent immunity to toxoplasmosis in the normal and immunocompromised guinea-pig host.
abstract: Guinea-pigs made T-cell deficient by thymectomy and irradiation, and protected with syngeneic bone marrow cells (TXB) have a greatly reduced capacity to express normal cell-mediated immune functions, based on their poor responses to T-cell mitogens, prolonged acceptance of skin allografts, and susceptibility to the lethal effects of graft-versus-host disease. Further evidence for impaired T-cell activity in TXB guinea pigs was based on their inability to be fully sensitized to mycobacterial antigens, and increased susceptibility to an intradermally induced infection with the intracellular protozoan parasite, Toxoplasma gondii (RH strain). After challenge at multiple sites with 10(6) or 10(5) parasites, toxoplasmosis in thymus-intact, fully immunocompetent guinea pigs is a self-limiting and survivable infection, whereas the disease takes an acutely lethal course in the majority of TXB guinea-pigs. The latter also had more parasites disseminating to various tissues sites than their euthymic counterparts. The reduced capacity of TXB guinea-pigs to respond to mycobacterial products, and to generate anti-Toxoplasma immunity can be restored by an intravenous infusion of normal syngeneic thymocytes. These findings provide substantial direct evidence strengthening the concept that protection against toxoplasmosis is heavily dependent upon an intact T-cell component of the host's immune response.
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D004195:Disease Models, Animal / Q000276:immunology; D004198:Disease Susceptibility; D006087:Graft vs Host Reaction; D006168:Guinea Pigs; D006790:Host-Parasite Interactions; D007111:Immunity, Cellular; D007153:Immunologic Deficiency Syndromes / Q000150:complications / Q000276:immunology*; D008221:Lymphoid Tissue / Q000473:pathology; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D013934:Thymectomy; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000150:complications / Q000276:immunology* / Q000469:parasitology