title: Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene.
abstract: A cis-acting regulatory sequence was identified upstream of the mouse H-2Kb class I major histocompatibility gene. Deletions in the H-2Kb promoter revealed that sequences located between 190 and 138 nucleotides upstream of the transcription initiation site contribute to basal gene expression as well as to stimulation by alpha-interferon. Furthermore, a nuclear factor found in several cell types binds with high affinity to a sequence centered 166 nucleotides upstream of the H-2Kb initiation site. In vivo competition experiments demonstrated that this factor plays a direct role in H-2Kb expression in mouse fibroblasts. The binding site for this factor is TGGGGATTCCCCA, a sequence of perfect dyad symmetry. This factor also binds a similar sequence in the 72-base-pair repeat enhancer element of simian virus 40.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002872:Chromosome Deletion; D005796:Genes; D005809:Genes, Regulator; D006183:H-2 Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D006183:H-2 Antigens; C032876:H-2Kb protein, mouse