Chemical synthesis of the T4 endonuclease V gene and its expression in Escherichia coli.
A gene coding for T4 endonuclease V was constructed by joining chemically-synthesized oligodeoxyribonucleotides. This gene was inserted into an expression vector and expressed in E. coli.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000138:chemical synthesis*; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005813:Genes, Synthetic; D005814:Genes, Viral; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; D004706:Endodeoxyribonucleases; C470698:endonuclease V, phage T4; D043224:Deoxyribonuclease (Pyrimidine Dimer)