Replacement of the anterior cruciate ligament with a bone-ligament-bone anterior cruciate ligament allograft in dogs.
Acute replacement of the canine anterior cruciate ligament (ACL) with a frozen, bone-ligament-bone anterior cruciate ligament preparation was studied using biochemical, immunologic, and biomechanical testing methods. Nine dogs were used for the study, six dogs received allografts and three received autografts. No tissue antigen matching was performed. All nine dogs were killed nine months after surgery. Necropsy examination revealed that the ACL was not present in three joints (one autograft, two allografts). The two autograft and four allograft ligaments available for mechanical testing sustained mean maximum loads that were 10% and 14%, respectively, of the mean maximum loads sustained by the contralateral ACL. Autoradiography indicated that cellular activity was more pronounced in the autograft specimens. Hydroxyproline uptake was 200% and 45% of normal in the autograft and allograft ligaments, respectively. Both autograft and allograft specimens were producing Type I collagen at the time of killing. Antidonor dog leukocyte antigen (DLA) antibody was detected in the synovial fluid taken at the time of killing from six of six dogs that received allografts and in zero of three dogs that received autografts.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D016025:Bone Transplantation; D004285:Dogs; D005615:Freezing; D006909:Hydroxyproline / Q000032:analysis; D007719:Knee Joint / Q000601:surgery*; D007962:Leukocytes / Q000276:immunology; D008022:Ligaments / Q000032:analysis / Q000637:transplantation*; D011309:Preservation, Biological; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D000906:Antibodies; D006909:Hydroxyproline