[In-vivo marking of human tumors using BUDR for flow-cytometry determination of kinetic cell parameters].
The flow-cytometry results of solid, human tumours are reported which were labelled with Bromodeoxyuridine in vivo. The preparation of the single cell suspension is prescribed. The S-phase percentage could be determined exactly in all cases but one. It is a rapid procedure which gives results in one day and the in vivo labelling had no adverse effects.
D001973:Bromodeoxyuridine / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D007399:Interphase; D008297:Male; D010051:Ovarian Neoplasms / Q000378:metabolism*; D012509:Sarcoma / Q000378:metabolism*
D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine
In-vivo-Markierung menschlicher Tumoren mit BUdR zur flusszytometrischen Bestimmung zellkinetischer Parameter.