[Proliferative activity and the efficacy of radiation therapy in melanoma and squamous-cell keratinizing cancer of the skin].
Proliferative activity (growth fraction) of malignant melanoma and keratinizing squamous cell carcinoma of the skin (19 patients) was studied using L. Schiffer's method (1975) modified by R. K. Karakulov (1982) and based on differences in the activity of DNA-polymerase in proliferating cells and cells "beyond the cycle". The number of active DNA-polymerase containing nuclei was determined using tumor cell nuclear DNA as matrices. The results obtained showed that malignant skin melanoma possessed a low proliferative activity (13.4% before radiation exposure). After the delivery of a dose of 30 Gy growth fraction was lowered by 25% (approximately 10%). Squamous cell skin carcinoma possessed a high proliferative activity, its basal value was 39.4%. After a dose of 30 Gy growth fraction was decreased 3-fold (approximately 12.9%). In malignant melanoma there was no correlation between a growth fraction value, the period and stage of disease as well as between a decrease in growth fraction and a degree of tumor regression by the end of irradiation. In skin cancer there was direct correlation between the depth of a growth fraction drop and a degree of tumor regression after a dose of 30 Gy. Therefore one can assume that the determination of a value of proliferative activity of radiosensitive tumors can serve as a criterion of therapeutic efficacy.
D000328:Adult; D000368:Aged; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000532:radiotherapy*; D002455:Cell Division / Q000528:radiation effects; D004583:Electrons; D006258:Head and Neck Neoplasms / Q000473:pathology / Q000532:radiotherapy; D006801:Humans; D007866:Leg; D008545:Melanoma / Q000473:pathology / Q000532:radiotherapy*; D008875:Middle Aged; D009367:Neoplasm Staging; D011300:Preoperative Care; D011879:Radiotherapy Dosage; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology / Q000532:radiotherapy*
Proliferativnaia aktivnost' i éffektivnost' luchevoĭ terapii melanomy i ploskokletochnogo orogovevaiushchego raka kozhi.