Computer controlled affinity chromatographical purification of soluble Plasmodium falciparum antigens from supernatants of in vitro cultures.
Affinity chromatographic procedures are difficult to scale up from the analytical to the preparative level when the ligand used for purification is a limiting factor. A versatile, computer-controlled affinity chromatographic system is described which permits automatic repetition of the purification process and sophisticated control functions based on the ultra-violet absorbance of fluid passing through the affinity column. The system has been used for automation and scaling up of the purification of Plasmodium falciparum exoantigens.
D000953:Antigens, Protozoan / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003201:Computers; D066298:In Vitro Techniques; D010963:Plasmodium falciparum / Q000276:immunology*
D000953:Antigens, Protozoan