Glucose tolerance factor stimulates 3-O-methylglucose transport into isolated rat adipocytes.
Glucose tolerance factor partially purified from yeast extract powder stimulated [U-14C]-D-glucose uptake to a level 5.6 times greater than the basal level in the absence of insulin in isolated adipocytes prepared from rats fed with normal laboratory chow. The factor also stimulated 3-O-methylglucose transport 2.2-fold from the basal level in the absence of insulin, but not in the presence of 8 nM insulin. Kinetic analysis revealed that glucose tolerance factor increased 3-O-methylglucose transport by decreasing the Ks value for 3-O-methylglucose with little change in the Vmax.
D019325:3-O-Methylglucose; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000596:Amino Acids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002857:Chromium / Q000302:isolation & purification / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D009539:Nicotinic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012441:Saccharomyces cerevisiae
D000596:Amino Acids; D007328:Insulin; D008757:Methylglucosides; D008759:Methylglycosides; D009539:Nicotinic Acids; C015240:glucose tolerance factor; D002857:Chromium; D019325:3-O-Methylglucose; D005947:Glucose