title: Perforated hepatocytes as a system for studying intracellular proteolysis.
abstract: Isolated rat hepatocytes were treated either by electroshock or enterotoxin in order to perforate the plasma membrane. Under appropriate conditions perforated cells could be incubated for a couple of hours without further disruption. The rate of proteolysis was higher in the perforated cells than in homogenized cells. ATP, a thiol reagent and Ca2+/Mg2+ stimulated proteolysis. By using inhibitors, it was shown that the proteolysis was partly lysosomal, and that all four classes of endoproteinases were involved.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D010447:Peptide Hydrolases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011506:Proteins; D000135:Acid Phosphatase; D010447:Peptide Hydrolases