Isolation and liquid chromatographic determination of the cyclic peptide mycotoxin cyclosporin A from rice.
A simple method for determination and quantitation of a cyclic peptide mycotoxin, cyclosporin A, in rice is presented. Rice inoculated with Trichoderma polysporum (Link ex Pers.) was extracted with methylene chloride after 4 weeks of incubation. Cyclosporin A was isolated from extracts by using open bed gel filtration column chromatography (LH-20, acetonitrile) and monitored with thin layer chromatography and reverse phase liquid chromatography coelution with a standard. Preliminary thin layer chromatographic methods were developed. Cyclosporin A was detected by iodine and after partial acid hydrolysis by ninhydrin and UV light. A liquid chromatographic method was developed that used a reverse phase disposable cartridge cleanup and isocratic chromatography with a reverse phase octadecylsilica column and a UV detector set at 212 nm. Recovery of cyclosporin A from spiked rice samples (mg/g range) was 85%.
D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003524:Cyclosporins / Q000032:analysis* / Q000302:isolation & purification; D005516:Food Microbiology; D007202:Indicators and Reagents; D012275:Oryza / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014242:Trichoderma / Q000378:metabolism
D003524:Cyclosporins; D007202:Indicators and Reagents