[Use of ganglioside-containing magnetic polyacrylamide sorbents for the immunoenzyme method of determining cholera enterotoxin].
A variant of EIA techniques for the determination of cholera enterotoxin is proposed. This method is based on the selective sorption of the toxin on ganglioside-containing magnetic granules with its subsequent detection by means of immune serum and antispecific immunoperoxidase conjugate. The proposed method permits the detection of 0.052 +/- 0.02 ng of protein of the purified toxin.
D000180:Acrylic Resins; D002772:Cholera Toxin / Q000032:analysis; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005732:Gangliosides; D005782:Gels; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007164:Immunosorbents; D008280:Magnetics; D012882:Skin Tests; D014734:Vibrio cholerae
D000180:Acrylic Resins; D004768:Enterotoxins; D005732:Gangliosides; D005782:Gels; D007164:Immunosorbents; C016680:polyacrylamide gels; C037420:stN protein, Vibrio cholerae; D002772:Cholera Toxin
Primenenie gangliozidsoderzhashchikh magnitnykh poliakrilamidnykh sorbentov dlia immunofermentnogo metoda oprdeleniia kholernogo énterotoksina.