Expression of c-sis and platelet-derived growth factor in in vitro-transformed glioma cells from rat brain tissue transplacentally treated with ethylnitrosourea.
Long-term culturing of brain cells from neonatal BD-IX rats after transplacental treatment with N-ethyl-N-nitrosourea (ENU) results in malignantly transformed cells after a lag period of about 250 days. During culturing, the brain cells undergo a sequence of morphological changes. We examined oncogene expression in cultured cells from ENU-treated animals and found that transformed glioma cells differ from premalignant glial cells by containing high levels of c-sis transcripts. We also report that the transformed cells synthesize functional platelet-derived growth factor. Because glial cells have receptors for platelet-derived growth factor, we propose that an autocrine mechanism plays an important role in ENU-induced brain tumorigenesis.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000473:pathology; D001932:Brain Neoplasms / Q000139:chemically induced / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005038:Ethylnitrosourea / Q000633:toxicity; D005260:Female; D005796:Genes; D005910:Glioma / Q000139:chemically induced / Q000235:genetics*; D008431:Maternal-Fetal Exchange; D009857:Oncogenes; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats
D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D005038:Ethylnitrosourea