title: Mode of disulfide bond formation of a heat-stable enterotoxin (STh) produced by a human strain of enterotoxigenic Escherichia coli.
abstract: To determine the modes of three disulfide linkages in the heat-stable enterotoxin (STh) produced by a human strain of enterotoxigenic Escherichia coli, we synthesized STh(6-18), which consists of 13 amino acid residues and has the same intramolecular disulfide linkages as native STh [(1985) FEBS Lett. 181, 138-142], by stepwise and selective formation of disulfide bonds using different types of removable protecting groups for the Cys residues. Synthesis of the peptide with different modes of disulfide bond formation provided three peptides consistent with standard STh(6-18) in their physicochemical and biological properties, thereby indicating that the disulfide bonds in STh(6-18) are Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Thr-Gly-Cys.
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000138:chemical synthesis / Q000378:metabolism* / Q000633:toxicity; D003553:Cystine / Q000032:analysis; D004768:Enterotoxins / Q000138:chemical synthesis / Q000378:metabolism* / Q000633:toxicity; D004926:Escherichia coli / Q000302:isolation & purification / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006801:Humans; D051379:Mice; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; C034151:heat stable toxin (E coli); D003553:Cystine