Expression of the art gene protein of human T-lymphotropic virus type III (HTLV-III/LAV) in bacteria.
Human T-lymphotropic virus type III (HTLV-III/LAV or HIV) contains a gene designated art (anti-repressor transactivator). Here, we report the expression of the art gene product in bacteria and show that the 20-kilodalton (kDa) bacterially expressed art protein is recognized by serum of a patient. The bacterially synthesized art protein competed in an immunological reaction with a 20-kDa protein produced in HTLV-III/LAV-infected lymphocytes. Antiserum to a synthetic oligopeptide corresponding to a sequence in the second exon of the art gene also precipitated the 20-kDa protein in HTLV-III/LAV-infected cells. These results demonstrate that the 20-kDa art gene product is expressed in cell lines that produce HTLV-III/LAV virions.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015694:Gene Products, rev; D005796:Genes; D005814:Genes, Viral; D006678:HIV / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D054321:rev Gene Products, Human Immunodeficiency Virus
D001426:Bacterial Proteins; D015694:Gene Products, rev; D014764:Viral Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus