title: Role of membrane N-linked oligosaccharides in host cell interaction with invasive forms of Trypanosoma cruzi.
abstract: The removal of N-linked oligosaccharides by peptide-N4-[N-acetyl-beta-glucoseaminyl]asparagine amidase (previously known as aspartoglycosylamine amidohydrolase and abbreviated N-glycanase) from the surface of blood or insect-transmissible forms of Trypanosoma cruzi markedly increased the capacity of these organisms to associate with (i.e., bind and penetrate) either mouse peritoneal macrophages or rat heart myoblasts. This effect was evidenced by a significant elevation in both the percentage of infected host cells and the average number of parasites per 100 cells. Conversely, N-glycanase treatment of either host cell markedly reduced both parameters to levels significantly below those obtained with cells mock treated with medium alone. The N-glycanase effect on the parasites was inhibited by heat inactivation of the enzyme or by the presence of fetuin, an N-glycanase substrate. The enhanced capacity of N-glycanase-treated T. cruzi to engage the host cells started to subside 2 h after the treatment, indicating the reversibility of the effect. The decreased reactivity of N-glycanase-treated macrophages or myoblasts with T. cruzi suggests that N-linked oligosaccharides on these host cells are involved in the initial phase of the cell infection process. Instead, because T. cruzi interacted more effectively with host cells after treatment with N-glycanase, parasite surface N-linked oligosaccharides would seem to interfere with the association.
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006321:Heart / Q000469:parasitology*; D006790:Host-Parasite Interactions; D008264:Macrophages / Q000469:parasitology*; D051379:Mice; D009206:Myocardium / Q000166:cytology; D009844:Oligosaccharides / Q000378:metabolism*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D051381:Rats; D014349:Trypanosoma cruzi / Q000378:metabolism / Q000502:physiology*
chemical_list: D009844:Oligosaccharides; D006026:Glycoside Hydrolases; C020668:glycanase; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase