title: [Fundamental studies on thyroid stimulating hormone immunoradiometric assay kit (TSH RIABEAD II)].
abstract: We have reported fundamental studies on the TSH immunoradiometric assay, using TSH RIABEAD II kit (Dainabot). The sensitivity of the assay was 0.03 mu IU/ml and its C.V. was 27.2%. Intra- and inter-assay C.V. were less than 5%. Dilution test and recovery test were good. Serum TSH level was 0.3-4.0 mu IU/ml in normal subjects, less than 0.03 mu IU/ml in untreated Graves' disease and subacute thyroiditis. Therefore, it was found that the clear difference exist in serum TSH levels between normal subjects and patients with untreated Graves' disease. There was a well correlation on the serum TSH levels between this method and TSH radioimmunoassay kit (Amerlex TSH, r = 0.983). Especially, the measurement of serum TSH levels, using immunoradiometric assay kit, was useful for the diagnosis of patients with Graves' disease.
mesh_terms: D005069:Evaluation Studies as Topic; D006111:Graves Disease / Q000097:blood; D006801:Humans; D007037:Hypothyroidism / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D013966:Thyroiditis / Q000097:blood; D013972:Thyrotropin / Q000097:blood*
chemical_list: D011933:Reagent Kits, Diagnostic; D013972:Thyrotropin