title: Studies on glutathione reductase and methemoglobin from human erythrocytes parasitized with Plasmodium falciparum.
abstract: An improved protein-blotting procedure and a thin layer isoelectric focusing technique are introduced to study glutathione reductase and methemoglobin (Met-Hb). According to our results, there is only one form of glutathione reductase in normal red blood cells. A similar protein was shown to be present at higher concentration in isolated merozoites. Both proteins have a subunit Mr of ca. 50,000 and react with anti-human glutathione reductase serum. Red cells with schizonts do not possess a higher proportion of Met-Hb than non-parasitized erythrocytes. This finding suggests that Met-Hb is not an indicator of metabolic alterations in malaria-infected erythrocytes.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis / Q000201:enzymology / Q000469:parasitology*; D005980:Glutathione Reductase / Q000097:blood*; D006801:Humans; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D008708:Methemoglobinemia; D010963:Plasmodium falciparum / Q000502:physiology*
chemical_list: D005980:Glutathione Reductase