title: Tissue distribution, developmental profile and hormonal regulation of androgen-responsive secretory proteins of rat seminal vesicles studied by immunocytochemistry.
abstract: The seminal vesicles of the rat synthesise large amounts of androgen-regulated secretory proteins. Indirect immunofluorescence cytochemistry and immunoblotting with monospecific polyclonal antibodies against three of the major secretory proteins (II, S and F) have been used to investigate the tissue distribution, subcellular localisation, androgen-regulation and developmental profile of secretory protein synthesis. There was no evidence for regional specialisation of the seminal vesicle epithelium; every epithelial cell synthesizes all three proteins via a classical secretory involving storage in secretory vesicles. Proteins S and II are contained within the same secretory vesicles. The time course of deinduction of proteins S and F after castration and their reinduction by testosterone closely followed that for their specific mRNAs described previously. During development, proteins S and F first appear between 10 and 15 days after birth. A protein immunologically related to seminal vesicle protein II is present in the lateral and dorsal lobes of the prostatic complex.
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D004848:Epithelium / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007159:Immunologic Tests; D008297:Male; D029584:Prostatic Secretory Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology; D014018:Tissue Distribution
chemical_list: D000728:Androgens; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D013739:Testosterone