title: Comparative evaluation of the Abbott TDX, the Abbott ABA200, and the Syva LAB5000 for assay of serum gentamicin.
abstract: Three automated chemical assays for serum gentamicin were compared for accuracy, reproducibility and cost. One method utilized fluorescence polarization (Abbott TDX), and the other two enzyme-multiplied immunoassay (Abbott ABA200, and the Syva Lab5000). All three systems produced a high degree of accuracy and reproducibility with spiked samples when the concentrations of gentamicin were within the range of 3.0-8.0 mg/l. However, with concentrations below 2.0 mg/l or above 8.0 mg/l, only the TDX system gave acceptable coefficients of variation and accurate recoveries. Similarly, excellent correlations were obtained between all three systems for assays of clinical specimens containing 2.0-8.0 mg/l gentamicin, but above and below this range, the correlations were poor except between TDX and Lab5000 within the range of 0.0-2.0 mg/l. The Abbott TDX was thus the most accurate and reproducible of the three systems for the assay of serum gentamicin in the critical concentrations below 2.0 mg/l and above 8.0 mg/l. The cost per assay by the Abbott TDX was US$4.57 compared with US$5.40 for the Abbott ABA200, and US$3.20 for the Syva Lab5000.
mesh_terms: D003365:Costs and Cost Analysis; D005069:Evaluation Studies as Topic; D005454:Fluorescence Polarization; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic
chemical_list: D005839:Gentamicins; D011933:Reagent Kits, Diagnostic