A simple assay for the detection of antibodies to endocrine islet cell surface antigens.
A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002478:Cells, Cultured; D006825:Hybridomas; D007457:Iodine Radioisotopes; D007515:Islets of Langerhans / Q000276:immunology*; D051381:Rats
D000906:Antibodies; D007457:Iodine Radioisotopes