A new Golgi method for adult human brains.
A new fast and reliable Golgi method to impregnate neurons in adult human brains which have been routinely fixed in neutral buffered formalin is described. In addition to potassium dichromate and sucrose, this technique uses microwave energy and hydrogen peroxide. Dendrites and perikarya of cortical pyramidal neurons and large subcortical multipolar neurons are the neuronal structures most commonly impregnated by this method.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D006056:Golgi Apparatus / Q000648:ultrastructure*; D006652:Histological Techniques; D006801:Humans; D002679:Pan troglodytes