title: A common positive trans-acting factor binds to enhancer sequences in the promoters of mouse H-2 and beta 2-microglobulin genes.
abstract: Using gel retardation and in vitro "footprinting", we have analyzed the interactions between nuclear proteins derived from various mouse cells and the enhancer and interferon response sequences of the H-2Kb gene. We have found that a protein factor binds a site in the enhancer sequence that partially overlaps the interferon response sequence. This factor also binds to a similar sequence lying in the opposite orientation in the promoter of the mouse beta 2-microglobulin gene, suggesting a common regulatory mechanism. Transfection competition experiments indicate that this factor acts as a positive element in the expression of H-2 and beta 2-microglobulin genes.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004742:Enhancer Elements, Genetic; D005796:Genes; D005809:Genes, Regulator; D006183:H-2 Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex; D051379:Mice; D011401:Promoter Regions, Genetic; D001613:beta 2-Microglobulin / Q000235:genetics*
chemical_list: D006183:H-2 Antigens; D001613:beta 2-Microglobulin