Autoradiographic localization of binding sites for 3H-serotonin and 3H-ketanserin on neurones and astrocytes of cultured rat brain stem and spinal cord.
By means of lightmicroscopic autoradiography we have studied the cellular localization of binding sites for 3H-serotonin and 3H-ketanserin in organotypic cultures of rat brain stem and spinal cord. In both types of cultures, a relatively great number of neurones revealed binding sites for 3H-serotonin which predominantly labels S1-receptors. 3H-ketanserin, an S2-antagonist was also bound to many neurones although to a lesser extent than 3H-serotonin. Binding sites for both radio-ligands were also observed on astrocytes. These findings together with electrophysiological investigations indicate that astrocytes possess serotonin receptors.
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001345:Autoradiography; D001665:Binding Sites; D001933:Brain Stem / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D007650:Ketanserin / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D011985:Receptors, Serotonin / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
D011985:Receptors, Serotonin; D012701:Serotonin; D007650:Ketanserin