Gonadotropin-releasing peptide from human follicular fluid: isolation, characterization, and chemical synthesis.
A gonadotropin-releasing peptide has been isolated from human follicular fluid. Its amino acid composition and sequence are completely different from the hypothalamic lutropin-releasing hormone. It is designated human follicular gonadotropin-releasing peptide and abbreviated as hF-GRP. The primary structure of this peptide (H-Thr-Asp-Thr-Ser-His-His-Asp-Gln-Asp-His-Pro-Thr-Phe-Asn-OH) has been confirmed by chemical synthesis. In the mouse pituitary incubation assay, the ED50 value for follitropin or lutropin release is estimated to be 1.2-1.6 nM.
D001826:Body Fluids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D006080:Ovarian Follicle / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification*; D010906:Pituitary Hormone-Releasing Hormones / Q000138:chemical synthesis / Q000302:isolation & purification*
D010455:Peptides; D010906:Pituitary Hormone-Releasing Hormones