Measurement of free and bound malondialdehyde in vitamin E-deficient and -supplemented rat liver tissues.
The quantity of free malondialdehyde (MDA) in liver tissues of rats fed vitamin E-deficient or -supplemented diets for 43 wk was measured by a newly developed high performance liquid chromatographic (HPLC) method. Bound MDA was quantified by the same HPLC method after alkaline hydrolysis of tissue homogenates. Tissues from vitamin E-deficient animals showed levels of free MDA about 15 times higher but levels of bound MDA less than 2 times higher than the vitamin E-supplemented animals. Free MDA is the major form in vitamin E-deficient tissues, but bound MDA is predominant in vitamin E-supplemented tissues. Conventional thiobarbituric acid (TBA) test results revealed that the content of TBA-reactive substances expressed in MDA equivalents was much higher than the actual free MDA levels in all groups. Results indicate that free MDA level measured by HPLC is a more sensitive index than the TBA value for lipid peroxidation. Some other TBA-reactive substances seem to exist in liver tissue regardless of the dietary treatment.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000032:analysis*; D008314:Malonates / Q000032:analysis*; D008315:Malondialdehyde / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates; D014810:Vitamin E / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000378:metabolism*
D008054:Lipid Peroxides; D008314:Malonates; D013858:Thiobarbiturates; D014810:Vitamin E; D008315:Malondialdehyde; C029684:thiobarbituric acid