The phagocytosis of yeast cells by monocytes: effects of colchicine, beta-lumicolchicine, and gamma-lumicolchicine.
The effect of microtubule antagonist (MA) colchicine and its two non-MA derivatives beta-lumicolchicine and gamma-lumicolchicine on the phagocytosis of yeast cells by glass-adherent monocytes were studied with a fluorescence quenching technique which allowed a distinction between the adherence and engulfment phases of phagocytosis. The engulfment of yeast cells was depressed by colchicine (p = 0.031) but not by the lumicolchicines. The adherence of yeast cells to the monocytes, on the other hand, was depressed by gamma-lumicolchicine (p = 0.028). The colchicine effect suggests that cytoplasmic microtubules play a role in the engulfment process whereas the lumicolchicine effect suggests a role of nucleoside transport over the cell membrane in the adherence process of phagocytosis.
D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003078:Colchicine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006801:Humans; D008162:Lumicolchicines / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000276:immunology
D008162:Lumicolchicines; D003078:Colchicine