Photochemical properties of Escherichia coli DNA photolyase: a flash photolysis study.
Escherichia coli DNA photolyase contains a stable flavin neutral blue radical that is involved in photosensitized repair of pyrimidine dimers in DNA. We have investigated the effect of illumination on the radical using light of lambda greater than 520 nm from either a camera flash or laser. We find that both types of irradiations result in the photoreduction of the flavin radical with a quantum yield of 0.10 +/- 0.02. While photoreduction with the camera flash is minimal in the absence of an electron donor (dithiothreitol), laser flash photolysis at 532 nm reduces the flavin to the same extent in the presence or absence or an electron donor. Thus, it is concluded that the primary step in photoreduction involves an electron donor that is a constituent of the enzyme itself. Laser flash photolysis produces a transient absorption band at 420 nm that probably represents the absorption of the lowest excited doublet state (2(1)IIII*) of the radical and decays with first-order kinetics with k1 = 0.8 X 10(6) s-1. The photoreduction data combined with the results of recent studies on the activity of dithionite-reduced enzyme suggest that electron donation by excited states of E-FADH2 is the mechanism of flavin photosensitized dimer repair by E. coli DNA photolyase.
D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D007700:Kinetics; D007834:Lasers; D008190:Lyases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010782:Photolysis
D005182:Flavin-Adenine Dinucleotide; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase