Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA.
A lambda gt10 library containing DNAs complementary to messenger RNAs from human breast cancer T47-D cells was constructed and screened with a cDNA probe encoding the rabbit progesterone receptor. Four overlapping clones have been sequenced. The open reading frame corresponds to a protein of 933 amino acids with a molecular weight of 98,868 Da. The cysteine rich basic region supposed to be involved in DNA binding is completely homologous in the human and rabbit receptors, whereas the C-terminal end, where hormone binding is thought to take place, differs by a single amino acid change. The human progesterone receptor is characterized, as is the rabbit receptor, by the very high proline content of its N-terminal region. When mRNAs from either human breast cancer cell lines T47-D and MCF-7 or from normal human uterus tissue were blotted and probed with the cloned cDNA, four main bands were observed (5100, 4300, 3700, and 2900 nucleotides).
D000595:Amino Acid Sequence; D001943:Breast Neoplasms; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000235:genetics; D011965:Receptors, Glucocorticoid; D011980:Receptors, Progesterone / Q000235:genetics; D014599:Uterus / Q000502:physiology
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D011980:Receptors, Progesterone; D004247:DNA