Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.
A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleotide splint, a single stranded 98mer was efficiently joined to the immobilized synthetic fragment. After impregnation of the latex microspheres with the fluorescent dye, Nile Red and attachment of an aldehyde 16mer, 5 X 10(5) bead-DNA conjugates could be detected with a conventional fluorimeter.
D000447:Aldehydes; D002264:Carboxylic Acids; D002851:Chromatography, High Pressure Liquid; D004247:DNA; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
D000447:Aldehydes; D002264:Carboxylic Acids; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA