A new experimental model of venous thrombosis in rats involving partial stasis and slight endothelium alterations.
Venous thrombus formation is induced in rats using saline flushing in a venous bag created in the vena cava and subsequent stasis by stricture of the vein. Maximum thrombus weight is obtained in 15 minutes. Neither flushing nor partial stasis alone could trigger thrombus formation during this time. Transmission electron microscopy demonstrated that flushing induces discrete endothelial lesions with no evident desendothelialisation areas. Anticoagulant agents are found to be active in preventing thrombus formation. However, heparin or related compounds such as pentosan polysulphate are active at dose levels not demonstrating anticoagulant activity. Thrombosis could be induced in thrombocytopenic rats indicating the non-involving of platelets. Moreover, aspirin did not prevent thrombus formation. Ticlopidine, another antiplatelet agent, did show an activity which might not involved platelets. This model thus possesses the features necessary for reliable investigation of potential antithrombotic agents for human use.
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D004727:Endothelium / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013924:Thrombophlebitis / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control
D000925:Anticoagulants; D001241:Aspirin