Characterization of Tritrichomonas foetus antigens, using bovine antiserum.
Tritrichomonas foetus antigens were identified, using the serum of an Angus heifer that had been repeatedly immunized with suspensions of 1 X 10(8) organisms in Freund's complete adjuvant. Antibody activity against T foetus was determined by dot-blot analysis, using horse-radish peroxidase-conjugated anti-bovine immunoglobulin to detect bound antibody. The antiserum contained antibodies against surface and flagellar components of live or fixed T foetus, as determined by use of immunofluorescence. The antiserum reacted with approximately 38 proteins in a pool of 55 to 60 components resolvable by polyacrylamide-gel electrophoresis of T foetus extracts.
D000818:Animals; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D011528:Protozoan Infections / Q000276:immunology; D011529:Protozoan Infections, Animal; D014318:Tritrichomonas / Q000276:immunology*
D000953:Antigens, Protozoan