[Effects of subinhibitory concentrations of pefloxacine on hemolysin production and adherence of urinary Escherichia coli].
The in vitro effect of subinhibitory concentrations of pefloxacin on E. coli's production of hemolysin and adherence to eucaryotic cells was studied. Six uropathogenic E. coli strains, with mannose-resistant and/or mannose-sensitive adhesins were tested. All strains produced an alpha-hemolysin and were susceptible to pefloxacin (0.125 less than or equal to MIC less than or equal to 0.250 mg/l). The effect on hemolysin production was studied using microtitration plates to determine the concentration inhibiting 50% of E. coli's hemolytic activity. The inhibition of adhesion was tested following adhesion of E. coli to uroepithelial cells. Sub-MICs of pefloxacin strongly inhibited the production of alpha-hemolysin and adhesion of E. coli regardless of adhesin type. A correlation between the inhibition of hemolysin production and the inhibition of adhesion to uroepithelial cells was found for four E. coli strains.
D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002648:Child; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000096:biosynthesis; D006801:Humans; D009643:Norfloxacin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015366:Pefloxacin; D014552:Urinary Tract Infections / Q000382:microbiology
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D015366:Pefloxacin; D009643:Norfloxacin
Action de concentrations subinhibitrices de péfloxacine sur la production d'hémolysine et le pouvoir d'adhérence d'Escherichia coli urinaires.