Genotoxicity of fecapentaene-12 in bacterial and mammalian cell assay systems.
Fecapentaene-12 (Fec-12), a compound thought to be responsible for much of the mutagenicity in fecal extracts from groups at high risk for colon cancer, was assayed for genotoxic potential in a battery of bacterial and mammalian cell short-term assays. This compound demonstrated significant mutagenic activity with Salmonella typhimurium tester strains TA104, TA100 and TA98, inducing approximately 1400, 700 and 100 revertants/micrograms, respectively. Fec-12 caused dose-dependent increases in unscheduled DNA synthesis in both rat hepatocytes and human fibroblasts, indicating its potential genotoxicity to mammalian as well as bacterial cells. Finally, Fec-12 had the ability to induce neoplastic transformation in mouse BALB/c 3T3 cells in the absence of exogenous metabolic activation.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D011090:Polyenes / Q000633:toxicity*; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects
D011090:Polyenes; C037357:1-(1-glycero)dodeca-1,3,5,7,9-pentaene; D004247:DNA