Reversal effect of isaxonine on modifications of rat polymorphonuclear migration by colchicine.
Using a modified Boyden chamber, random migration and chemotaxis of rat polymorphonuclear leucocytes (PMN) were assessed after incubation in vitro in a solution of colchicine (1.5 X 10(-5) M). The results obtained were compared to random migration or chemotaxis of the same cellular pool treated in vitro or in vivo by N-isopropyl-amino-2-pyrimidine phosphate (isaxonine 4.10(-5) M or 75 and 150 mg/kg respectively). Isaxonine partially inhibited the effect of colchicine on PMN chemotaxis while random migration remained unaffected. Isaxonine might partially inhibit the noxious effect of colchicine on microtubule disruption by a direct effect (polymerization of free tubulin) and/or indirect effect (impairment of disorganizing activity) on PMN microtubules.
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003078:Colchicine / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D008297:Male; D008870:Microtubules / Q000187:drug effects; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects*; D011743:Pyrimidines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D011743:Pyrimidines; D009240:N-Formylmethionine Leucyl-Phenylalanine; C020375:isaxonine; D003078:Colchicine