[Determination of lymphokine-induced cytolytic activity of macrophages by measuring the 3H-thymidine residue in prelabeled tumor cells].
A technique of macrophage-activating factors (MAF) detection by the in vitro determination of macrophage (Mph) antitumour cytolytic activity by 3H-thymidine residue in the pre-labelled neoplastic target cells (TC) is suggested. Peptone-induced Mph were cultivated for 20-22 hours in the presence of crude supernatants from concanavalin-A-stimulated spleen cells or from the secondary mixed lymphocyte culture. 3H-thymidine-labelled cells of mastocytoma P815 were then added to the washed Mph. The lysis was measured in 48 hours according to the isotope remainder in the non-acid-soluble fraction. The optimal conditions for MAF detection have been selected. Parallel MAF testing with this particular method and with a standard technique using labelled 51Cr TC made it possible to conclude that the method suggested is more sensitive because it permits a combined cultivation of Mph and TC for a relatively long time period.
D000818:Animals; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D008222:Lymphokines / Q000032:analysis*; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000276:immunology*; D013936:Thymidine / Q000032:analysis
D008222:Lymphokines; D016215:Macrophage-Activating Factors; D003208:Concanavalin A; D013936:Thymidine
Opredelenie indutsirovannoĭ limfokinom tsitoliticheskoĭ aktivnosti makrofagov po ostatku 3H-timidina v predvaritel'no mechennykh kletkakh opukholi.