title: Adenosine deaminase-containing hypothalamic neurons accumulate 5-hydroxytryptophan: a dual-colour immunofluorescence procedure using a new fluorescence marker.
abstract: Neurons of the posterior hypothalamic magnocellular nucleus (PHMN) in the rat are immunoreactive for adenosine deaminase (ADA) and cells coextensive with this nucleus show immunoreactivity for serotonin after pretreatment with 5-hydroxytryptophan (5-HTP). The degree to which ADA coexists in cells having 5-HTP uptake capability was analyzed by a new double immunohistofluorescence procedure employing diethylaminocoumarin (DAMC). It was determined that 5-HTP accumulating cells of the PHMN represent a subpopulation of those immunoreactive for ADA. These results add further to the neurochemical characterization of this globally projecting hypothalamic cell group and point out the utility of DAMC as a new immunohistofluorescence marker.
mesh_terms: D006916:5-Hydroxytryptophan / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D005455:Fluorescent Antibody Technique; D007031:Hypothalamus / Q000378:metabolism*; D007034:Hypothalamus, Posterior / Q000378:metabolism*; D008297:Male; D009700:Nucleoside Deaminases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism
chemical_list: D012701:Serotonin; D006916:5-Hydroxytryptophan; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase