title: The demonstration of rabies antigen in paraffin-embedded tissues using the peroxidase-antiperoxidase method: a comparative study.
abstract: Mice experimentally infected with challenge virus standard rabies virus as well as skunks and foxes experimentally infected with street rabies virus were used to demonstrate rabies viral antigen in paraffin-embedded tissue by the peroxidase-antiperoxidase method. Tissues fixed with different fixatives (10% formalin, Bouin's, acetone, ethanol) for various times and fresh frozen tissues were stained by the fluorescent antibody and the peroxidase-antiperoxidase method. Formalin- and Bouin's-fixed tissues were tested with and without use of digestive enzyme (pepsin). The results demonstrated that a procedure using formalin-fixed paraffin-embedded tissue treated with pepsin and stained by peroxidase-antiperoxidase was the best method for both preservation of morphological details and demonstration of antigen.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001921:Brain / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005589:Foxes; D005615:Freezing; D007124:Immunoenzyme Techniques; D012889:Mephitidae; D051379:Mice; D009474:Neurons / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*
chemical_list: D000956:Antigens, Viral