Differentiation-associated decrease in muscarinic receptor sensitivity in human neuroblastoma cells.
Muscarinic receptor-linked increases in intracellular free Ca2+ as measured with quin-2 and Ca2+ release from monolayers of cells have been measured in the human neuroblastoma cell line SH-SY5Y. Induction of differentiation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to a decrease in the sensitivity of the cells to low concentrations of agonists with respect to the induced increase in cytosolic free Ca2+ and stimulation of Ca2+ efflux. No decrease in agonist binding affinity was observed when the displacement of a labelled antagonist, 3H-NMS, by a non-labelled agonist was studied.
D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D007700:Kinetics; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology; D019832:N-Methylscopolamine; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012602:Scopolamine Derivatives / Q000378:metabolism
D008688:Methacholine Compounds; D011976:Receptors, Muscarinic; D012602:Scopolamine Derivatives; D016210:Methacholine Chloride; D002118:Calcium; D019832:N-Methylscopolamine