Antigenic pattern of human brain glycoproteins as described by monoclonal antibodies.
A battery of monoclonal antibodies was raised against a preparation of lentil lectin-binding membrane glycoproteins from human brain. Out of 26 established hybridomas, nine produced antibodies against the human Thy-1 antigen. For the remaining 17 lines, reactivity with at least six other antigens could be identified after immunoprecipitation and immunoblotting. Several of the antigens were di- or trimeric, mainly in the molecular weight range of 60-120 kDa. Two of the antibodies were reactive with high-molecular-weight aggregates and four targets for the antibody reactivity were not identifiable by immunoprecipitation of iodinated antigens. Three of the identified antigens were shown by quantitative enzyme-linked immunosorbent assay tests on various human tissues to be specifically expressed in the brain.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D001921:Brain / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007159:Immunologic Tests; D007163:Immunosorbent Techniques; D037102:Lectins / Q000378:metabolism; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000941:Antigens; D006023:Glycoproteins; D037102:Lectins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D037121:Plant Lectins; C033619:lentil lectin