Demonstration of immunohistochemical and immunochemical cross-reactivity of L-histidine and L-dopa decarboxylases using antibodies against the two enzymes.
Both rat L-histidine decarboxylase (HDC) and guinea-pig L-DOPA decarboxylase (DDC) were shown immunohistochemically and immunochemically to react with anti-rat HDC antibody. No cross-reaction was observed in immunoprecipitation experiments, but both anti-rat HDC antibody and anti-rat DDC antibody immunostained neurons in the substantia nigra, raphe nucleus and locus coeruleus of guinea-pig brain. Moreover, on immunoblotting, anti-rat HDC antibody recognized not only rat HDC but also guinea-pig DDC, but not rat DDC. However, anti-rat DDC antibody showed no immunohistochemical or immunochemical cross-reactivity with rat HDC.
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000276:immunology*; D001921:Brain / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000276:immunology*; D011232:Chemical Precipitation; D004296:Dopa Decarboxylase / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006640:Histidine Decarboxylase / Q000276:immunology*; D007034:Hypothalamus, Posterior / Q000201:enzymology; D007163:Immunosorbent Techniques; D051381:Rats; D013378:Substantia Nigra / Q000201:enzymology
D002262:Carboxy-Lyases; D004296:Dopa Decarboxylase; D006640:Histidine Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases