title: Functioning of the F-plasmid origin of replication in an Escherichia coli K12 Hfr strain during exponential growth.
abstract: The pattern of chromosome replication in the Escherichia coli K12 Hfr strain KL99 was investigated during exponential growth by DNA-DNA hybridization. The levels of chromosomal markers close to the point of insertion of F (near pyrC) were raised in relation to other markers by comparison with the situation in an isogenic F- strain. The data are shown to be consistent with the proposal that the integrated F plasmid was regulating its copy number by a mass-titration mechanism.
mesh_terms: D002455:Cell Division; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005784:Gene Amplification; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012093:Replicon
chemical_list: D004269:DNA, Bacterial