Monoclonal antibody 7D5 raised to cytochrome b558 of human neutrophils: immunocytochemical detection of the antigen in peripheral phagocytes of normal subjects, patients with chronic granulomatous disease, and their carrier mothers.
We have established a monoclonal hybridoma clone that produces IgG1 against the cytochrome b558 of human neutrophils. The antibody 7D5, secreted by the hybridoma, bound to solubilized cytochrome b of the neutrophils but not to other proteins such as hemoglobin, myeloperoxidase, and pig cytochrome P-450. Immunocytochemical studies of normal human peripheral blood showed that 7D5 bound to neutrophils and monocytes but not to lymphocytes or erythrocytes. The neutrophils of male patients but not of a female patient with chronic granulomatous disease lacked the antigen of 7D5 as well as the absorption spectrum for cytochrome b558. A mosaic of the antigen-positive and -negative neutrophils was observed in mothers of the male patients. These biochemical and immunocytochemical results indicate that 7D5 is a specific antibody against cytochrome b558 of human phagocytes.
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis; D003573:Cytochrome b Group / Q000032:analysis* / Q000276:immunology; D005260:Female; D006580:Genetic Carrier Screening; D006105:Granulomatous Disease, Chronic / Q000097:blood / Q000235:genetics* / Q000276:immunology; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D009000:Monocytes / Q000166:cytology; D019255:NADPH Oxidases; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D010586:Phagocytes / Q000166:cytology / Q000276:immunology*; D012016:Reference Values
D000911:Antibodies, Monoclonal; D000941:Antigens; D003573:Cytochrome b Group; C036888:cytochrome b558; D019255:NADPH Oxidases