[Detection of a system of microsomal monooxygenases in the rodent malaria parasite Plasmodium berghei].
A microsomal fraction from the cells of the malaria parasite of rodent Plasmodium berghei was obtained. The spectral properties of microsomal preparations suggest that P. berghei microsomes contain cytochromes b5 and P-420. Electrophoretic separation of microsomal proteins revealed the presence of proteins whose molecular mass corresponds to NADPH-cytochrome c reductase, cytochrome P-450 and epoxide hydratase. The activities of NADPH-cytochrome c reductase and benzpyrene hydroxylase were determined. The spectral parameters, electrophoretic data and enzymatic activities of microsomal proteins indicate that P. berghei cells contain a cytochrome P-450 monooxygenase system. The interrelationship between the activity of the microsomal monooxygenase system and the resistance of P. berghei cells to the antimalaria preparation chloroquine is discussed.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004352:Drug Resistance, Microbial; D008861:Microsomes / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009115:Muridae / Q000469:parasitology*; D010962:Plasmodium berghei / Q000187:drug effects / Q000201:enzymology*
D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases
Vyiavlenie sistemy mikrosomal'nykh monooksigenaz u maliariĭnogo parazita gryzunov Plasmodium berghei.