Aggregation of bilirubin in injectates and incubation media: its significance in experimental studies of CNS toxicity.
In experimental work with bilirubin toxicity, incubates or injectates are prepared without added albumin, or with a molar excess of bilirubin over albumin. Bilirubin is liable to aggregation as free bilirubin acid under these circumstances, giving rise to variable toxicity. Stable solutions are obtained with a molar excess of albumin over bilirubin; such solutions show no toxicity, however. It is suggested that this difficulty may be circumvented by using an excess of albumin, adding a sulfonamide as a bilirubin displacer, to provoke toxicity.
D000818:Animals; D001663:Bilirubin / Q000008:administration & dosage / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007567:Jaundice, Neonatal / Q000139:chemically induced*; D012709:Serum Albumin / Q000008:administration & dosage; D013449:Sulfonamides / Q000008:administration & dosage
D012709:Serum Albumin; D013449:Sulfonamides; D001663:Bilirubin