title: [Analysis of mutations affecting the expression of catabolite-sensitive operons in Escherichia coli K12 mutants defective in the HPr-component of the carbohydrate transport system].
abstract: Expression of catabolite-sensitive operons in mutants devoid of HPr (a component of the glucose transport system) is severely repressed. ptsH mutants do not utilize substrates of the phosphoenolpyruvate: carbohydrate system (PTS) and many other sugars. Analysis of mutations suppressing the effect of the delta ptsH mutation revealed a new class of reversions which restore the growth of bacteria on different substrates. This mutation (named ptsS) intensifies the growth rate of ptsH mutants and increases the differential rate of beta-galactosidase production. ptsS mutation was mapped in the region of ptsF gene (coding for the fructose specific enzyme II of the PTS) on the 46th min. of the E. coli chromosome map. The effect of the ptsS mutation on the expression of catabolite-sensitive operons manifests only in the presence of the intact enzyme I of the PTS.
mesh_terms: D001692:Biological Transport; D050260:Carbohydrate Metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D009154:Mutation; D009876:Operon; D013489:Suppression, Genetic
vernacular_title: Analiz mutatsiĭ, vliiaiushchikh na vyrazhennost' katabolitchuvstvitel'nykh operonov u mutantov Escherichia coli K12, lishennykh HPr-komponenta sistemy transporta uglevodov.