Antibodies to the ras gene product inhibit adenylate cyclase and accelerate progesterone-induced cell division in Xenopus laevis oocytes.
Microinjection of monoclonal antibodies (lines 238, 172, and 259) directed against the ras gene product, p21, into Xenopus laevis oocytes accelerated progesterone-induced germinal vesicle breakdown. Antibody 238 had the greatest effect on the acceleration of progesterone-induced oocyte maturation, and this effect was correlated with in vitro inhibition of adenylate cyclase (EC 4.6.1.1) activity in a concentration-dependent manner. Inhibition of adenylate cyclase by antibody 238 was also measured in membranes prepared from oocytes pretreated with either cholera toxin or pertussis toxin. These results suggest a role for the ras gene product in the regulation of vertebrate cell adenylate cyclase activity.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000235:genetics*; D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005796:Genes; D008845:Microinjections; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D011374:Progesterone / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000276:immunology; D016283:Proto-Oncogene Proteins p21(ras); D011519:Proto-Oncogenes; D014981:Xenopus
D000067956:Adenylyl Cyclase Inhibitors; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D011518:Proto-Oncogene Proteins; D011374:Progesterone; D016283:Proto-Oncogene Proteins p21(ras); D000262:Adenylyl Cyclases