Analysis by BrUdR-labelling technique of induced aneuploidy in mammalian cells in culture.
To study the origin of induced aneuploid cells, the BrUdR-labelling technique was applied to V79/AP4 Chinese hamster cells treated with colcemid or benomyl. In this way we were able to recognize the cells which had undergone one cellular division after the treatment since their chromosomes exhibited sister-chromatid differentiation. The results showed that the induced aneuploid cells can have either a few or numerous additional chromosomes depending on the concentrations of the drug. Moreover, it could be established that aneuploid cells with numerous additional chromosomes were obtained mainly when polyploid cells were also present in the treated population. This strongly suggests that the excess of additional chromosomes found in the aneuploid cells induced by the highest concentrations may be derived by disturbances of the whole mitotic apparatus rather than by a multiplicity of errors affecting individual chromosomes.
D000782:Aneuploidy; D000818:Animals; D001542:Benomyl / Q000494:pharmacology*; D001973:Bromodeoxyuridine; D002219:Carbamates / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003703:Demecolcine / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects
D002219:Carbamates; D001973:Bromodeoxyuridine; D001542:Benomyl; D003703:Demecolcine