Isolation and properties of two rat plasma T-kininogens.
Two species of T-kininogen which release T-kinin (Ile-Ser-bradykinin) have been purified from plasma of rats treated with Freund's complete adjuvant. The molecular weight was estimated to be 69,000 for either T-kininogen I and II by SDS-polyacrylamide gel electrophoresis. Trypsin released one mole of T-kinin from one mole of either T-kininogen, but glandular kallikrein, including rat urinary and rat submandibular gland kallikreins and human urinary kallikrein, did not release any kinin from T-kininogens. Cathepsin D, which was purified from rat liver, released T-kinin from T-kininogens at pH 4.0. These results indicate that rat plasma contains two types of T-kininogen which differ from high molecular weight and low molecular weight kininogens.
D000818:Animals; D006801:Humans; D007610:Kallikreins / Q000378:metabolism; D007700:Kinetics; D007704:Kininogens / Q000097:blood* / Q000302:isolation & purification; D007705:Kinins / Q000097:blood*; D010447:Peptide Hydrolases; D051381:Rats; D013363:Submandibular Gland / Q000201:enzymology; D014357:Trypsin / Q000378:metabolism
D007704:Kininogens; D007705:Kinins; D010447:Peptide Hydrolases; D007610:Kallikreins; D014357:Trypsin