Influence of embedding media in prolactin labelling with immunogold techniques.
Immunogold labelling of prolactin in three different embedding media was compared. The polymeric prolactin in secretory granules was labelled in the three media, however, acrylic monomers (Lowicryl K4M and LR White) provided a more intense labelling with higher dilutions of the primary antibody, compared to the labelling in the epoxy resin (araldite). An intense labelling of monomeric prolactin in Golgi complex was detected only in acrylic embedments, and the labelling on the rough endoplasmic reticulum was significant only in LR White embedded tissues.
D000180:Acrylic Resins; D000818:Animals; D004853:Epoxy Resins; D005260:Female; D006046:Gold; D006651:Histocytochemistry; D007158:Immunologic Techniques; D010795:Phthalic Acids; D010796:Phthalic Anhydrides; D010902:Pituitary Gland / Q000032:analysis; D011388:Prolactin / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000180:Acrylic Resins; D004853:Epoxy Resins; D010795:Phthalic Acids; D010796:Phthalic Anhydrides; C005752:araldite; D006046:Gold; C037883:Lowicryl 4KM resin; D011388:Prolactin; C048707:LR white