[Effect of dihydrocytochalasin B on the induction of DNA synthesis by epidermal growth factor, insulin and serum in Swiss 3T3 cells].
The addition of a microfilament-disorganizing agent--dihydrocytochalasin B B (5-10 micrograms/ml)--to to quiescent confluent or sparse (in 0.5% serum) Swiss 3T3 cells, 1-2 hours prior to stimulation, inhibited the initiation of DNA synthesis induced by an epidermal growth factor (7.5-10 ng/ml) and insulin (0.5-1.0 micrograms/ml), but exerted a low effect on serum stimulation. DNA synthesis was measured 21-23 hours after the growth factor administration by 14C-thymidine incorporation in acid-insoluble material and the ratio of this fraction to exogenous thymidine uptake. Moreover, the polar solvent dimethylsulfoxide, present in culture medium at low concentration (0.1-0.5%), also caused a decrease in the basal level of 14C-thymidine incorporation in resting cells, and a less decrease in the induced incorporation.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004347:Drug Interactions; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007106:Immune Sera / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D013997:Time Factors
D007106:Immune Sera; D007328:Insulin; C024794:dihydrocytochalasin B; D003571:Cytochalasin B; D004815:Epidermal Growth Factor; D004247:DNA; D004121:Dimethyl Sulfoxide
Vliianie digidrotsitokhalazina B na induktsiiu sinteza DNK épidermal'nym faktorom rosta, insulinom i syvorotkoĭ v kletkakh Swiss 3T3.