Interaction of piperine with rat liver microsomes.
Pretreatment of adult male albino rats with piperine did not induce cytochrome P-450 whereas safrole did cause such induction. Piperine and safrole interacted with different forms of cytochrome P-450 as indicated by their in vivo effect on drug metabolising enzymes and mixed function oxidases and electrophoretic patterns. Incubation of piperine or safrole with dithionite- or NADPH-reduced microsomes from untreated rats resulted in a modified type II difference spectrum with an absorption maximum at 427 nm and a trough at 408 nm.
D000470:Alkaloids; D000818:Animals; D052117:Benzodioxoles; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D010880:Piperidines / Q000633:toxicity*; D053284:Polyunsaturated Alkamides; D051381:Rats; D011919:Rats, Inbred Strains; D012451:Safrole / Q000633:toxicity
D000470:Alkaloids; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D003577:Cytochrome P-450 Enzyme System; D012451:Safrole; C008922:piperine