Two types of Newcastle disease viruses isolated from feral birds in western Australia detected by monoclonal antibodies.
Thirteen viruses isolated from feral birds and one isolated from a domestic duck, obtained in 1979-1980 during a survey of birds in Western Australia, were shown to be Newcastle disease viruses of low virulence for chickens. The binding of mouse monoclonal antibodies, raised against NDV-Ulster 2C to MDBK cells infected with the isolates was assessed using an indirect immunoperoxidase test. Five viruses caused binding of all 9 monoclonal antibodies tested, whereas the other 9 isolates induced binding of only 4 monoclonal antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal; D001717:Birds / Q000382:microbiology*; D002645:Chickens; D004372:Ducks; D006385:Hemagglutination Inhibition Tests; D007124:Immunoenzyme Techniques; D009521:Newcastle Disease / Q000382:microbiology; D009522:Newcastle disease virus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology; D014774:Virulence
D000911:Antibodies, Monoclonal