title: Effect of serum sources and colostral whey on bovine semen quality and spermatozoa immunoglobulin G immunofluorescence.
abstract: Heifer, bull, fetal calf sera, and colostral whey were used to evaluate the influence of protein concentrations on percent progressive motility, head-to-head agglutination, acrosomal integrity, and immunoglobulin G immunofluorescence of bovine spermatozoa using ejaculates from 10 bulls. In the first experiment, 10% (vol/vol) addition of undiluted colostral whey resulted in the highest head-to-head agglutination, acrosomal integrity, and immunoglobulin G immunofluorescence. Ten percent (vol/vol) addition of whey diluted to a protein concentration equivalent to fetal calf serum produced significantly lower agglutination, acrosomal integrity, and immunoglobulin G immunofluorescence. Fetal calf serum was unable to produce agglutination and immunoglobulin G immunofluorescence of bovine spermatozoa. Heifer and bull sera produced similar responses for all seminal measurements. In Experiment 2, unheated whey and heifer serum resulted in higher response for all variables than heat inactivated whey and heifer serum. Whey treatment produced greater spermatozoal motility, agglutination, acrosomal integrity; and immunoglobulin G immunofluorescence than treatment with heifer serum. Spermatozoal immunofluorescence indicated antibodies in normal whey, bull, and heifer serum bound to spermatozoal membranes at the acrosomal region. Colostral whey was an effective source of agglutinin factor. Normal unheated whey and heifer serum did not cause sperm damage or immobilization.
mesh_terms: D000818:Animals; D001798:Blood Proteins; D002417:Cattle / Q000502:physiology*; D003126:Colostrum / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis* / Q000276:immunology
chemical_list: D001798:Blood Proteins; D007074:Immunoglobulin G