title: Evaluation of radioimmunoassays: comparison of dose interpolation calculations by four parameter logistic and spline functions.
abstract: Two computerized methods for dose interpolation calculation were compared. Generated data sets with a known coefficient of variation as well as laboratory RIA data were analysed. The four parameter logistic method, which is based on an approximation of the mass action law, performed better than the Spline method, a procedure which makes no a priori assumptions about the data. Correct weighting of the data was important for obtaining satisfactory fits. The determination of the response error relationship proved to be the most satisfactory approach in obtaining suitable weighting factors.
mesh_terms: D001708:Biopterins / Q000031:analogs & derivatives / Q000097:blood; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007328:Insulin / Q000097:blood; D008432:Mathematical Computing; D009010:Monte Carlo Method; D019798:Neopterin; D009716:Numerical Analysis, Computer-Assisted; D011786:Quality Control; D011863:Radioimmunoassay
chemical_list: D007328:Insulin; D001708:Biopterins; D019798:Neopterin