title: Phorbol ester stimulates proliferation of astrocytes in primary culture.
abstract: Near-confluent primary cultures of astrocytes from the neonatal rat cerebral cortex were transferred to low serum (0.1%) growth medium for 24 h before a single addition of phorbol-12-myristate-13-acetate (0.01-100 ng X ml-1), a phorbol ester which mimics diacylglycerol activation of protein kinase C. After 48 h the cultures were pulsed with [methyl-3H]thymidine. Cultures exposed to phorbol ester exhibited dose-dependent increases in thymidine incorporation which were reversed by amiloride.
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000166:cytology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007328:Insulin / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D007328:Insulin; D000584:Amiloride; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine