Endotoxin increases pulmonary vascular protein permeability in the dog.
Endotoxin increases pulmonary vascular permeability consistently in some species but fails to reliably cause injury in the dog. We wondered whether this phenomenon depended on the method of injury assessment, as others have relied on edema measurement; we quantified injury by monitoring the rate of extravascular protein accumulation. 113mIn-labeled protein and 99mTc-labeled erythrocytes were injected into anesthetized dogs and monitored by an externally placed lung probe. A protein leak index, the rate of extravascular protein accumulation, was derived from the rate of increase in lung protein counts corrected for changes in intravascular protein activity. After administration of Salmonella enteriditis endotoxin (4 micrograms/kg), the protein leak index was elevated 2.5-fold (41.1 +/- 4.6 X 10(-4) min-1) compared with control (16.0 +/- 2.8 X 10(-4) min-1). In contrast, wet-to-dry weight ratios failed to increase after endotoxin (4.6 +/- 0.8 vs. control values of 4.2 +/- 0.5 g/g dry bloodless lung). However, we observed that endotoxin increased lung dry weight (per unit body weight), which may have attenuated the change in wet-to-dry weight ratios. To determine whether low microvascular pressures following endotoxin attenuated edema formation, we increased pulmonary arterial wedge pressures in five dogs by saline infusion, which caused an increase in wet-to-dry weight ratios following endotoxin but no change in the five controls. We conclude that low dose endotoxin causes pulmonary vascular protein leak in the dog while edema formation is minimal or absent.
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D002199:Capillary Permeability / Q000187:drug effects*; D004285:Dogs; D004731:Endotoxins; D004768:Enterotoxins / Q000633:toxicity*; D004912:Erythrocytes / Q000502:physiology; D007204:Indium; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D011506:Proteins / Q000378:metabolism*; D011652:Pulmonary Circulation / Q000187:drug effects*; D011868:Radioisotopes; D012477:Salmonella enteritidis; D013667:Technetium
D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; D011506:Proteins; D011868:Radioisotopes; C027952:salmonella toxin; D007204:Indium; D013667:Technetium