Enhancement of amyloid induction by amyloid fibril fragments in hamster.
An extract (fibril amyloid-enhancing factor) prepared by sonication of a suspension of water-purified hamster AA-amyloid fibrils showed an amyloid-enhancing effect upon intraperitoneal injection into hamsters. The fibril amyloid-enhancing factor was identical to the original fibrils according to the results of infrared spectroscopy, gel filtration, gel electrophoresis, and Western blotting; although lyophilized samples of the extract did not show any green birefringence after staining with Congo red. From these results, it was concluded that fibril amyloid-enhancing factor represents small fragments of amyloid fibrils which enhance in vivo formation of amyloid fibrils.
D000682:Amyloid / Q000096:biosynthesis / Q000502:physiology*; D000686:Amyloidosis / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification / Q000502:physiology*; D007158:Immunologic Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D013055:Spectrophotometry, Infrared; D013154:Spleen / Q000378:metabolism
D000682:Amyloid; D006023:Glycoproteins; C046748:amyloid enhancing factor