The mRNA coding for the secreted protease transin is expressed more abundantly in malignant than in benign tumors.
Transin RNA is a 1.9-kilobase RNA transcript induced by oncogenes in rat embryo fibroblast cell lines. We show that RNA species complementary to a cloned transin cDNA are present in mouse skin squamous cell carcinomas induced by a classical initiation-promotion protocol but not in premalignant, benign papillomas or in normal epidermis. A single application of a tumor promoting phorbol ester to normal epidermis elicits a transient increase in these RNA levels. Transin RNA encodes a secreted protease, an activity consistent with a functional role for enhanced expression of transin RNA in the progression of benign, encapsulated tumors to malignant, invasive carcinomas.
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D002472:Cell Transformation, Viral; D010450:Endopeptidases / Q000235:genetics*; D005786:Gene Expression Regulation; D019278:Matrix Metalloproteinase 3; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D010212:Papilloma / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics*; D012878:Skin Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D010450:Endopeptidases; D019278:Matrix Metalloproteinase 3; D013755:Tetradecanoylphorbol Acetate