Synthesis of 5'-phosphatidylnucleosides by phospholipase D-catalyzed transphosphatidylation.
Phospholipase D from Streptomyces effectively catalyzed the transfer reaction of the phosphatidyl residue from phosphatidylcholine to the 5'-hydroxyl group of nucleosides. A variety of 5'-phosphatidylnucleosides were prepared by this reaction in high yields. Some of them showed marked antitumor activities in mice.
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000138:chemical synthesis*; D007941:Leukemia P388 / Q000188:drug therapy; D051379:Mice; D009711:Nucleotides / Q000138:chemical synthesis* / Q000627:therapeutic use; D010712:Phosphatidic Acids / Q000138:chemical synthesis* / Q000627:therapeutic use; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology; D013329:Structure-Activity Relationship
D000964:Antimetabolites, Antineoplastic; D009711:Nucleotides; D010712:Phosphatidic Acids; D010740:Phospholipases; D010739:Phospholipase D