Effects of cytochalasin E on H+ and volume secretion in gastric fistula rats.
Effects of cytochalasin E on the secretion of H+ and volume of gastric juice were investigated in gastric fistula rats. Direct exposure of the gastric mucosa to cytochalasin E (5-20 micrograms/ml) inhibited H+ secretory rate in all of the pentagastrin-, histamine- as well as non-stimulated rats but it did not affect the secretory volume of gastric juice. The inhibitory action of cytochalasin E on H+ secretion had a rapid onset and showed a dose-dependent pattern. The mechanism of inhibitory action was not related to its interaction with plasma membrane receptors or membrane enzyme, K+-stimulated ATPase. Interference with cellular energy production as well as alteration of microfilament function, which are essential for H+ production and secretion respectively, are likely to be the bases of the cytochalasin inhibition.
D000818:Animals; D003572:Cytochalasins / Q000494:pharmacology*; D005744:Gastric Acid / Q000378:metabolism*; D005747:Gastric Fistula / Q000503:physiopathology*; D005750:Gastric Juice / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects; D006859:Hydrogen; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D003572:Cytochalasins; C007215:cytochalasin E; D006859:Hydrogen