title: The immunopathology of acute experimental allergic encephalomyelitis. IV. An ultrastructural immunocytochemical study of class II major histocompatibility complex molecule (Ia) expression.
abstract: Cell surface expression of Class II major histocompatibility complex (Ia) molecules is required for antigen recognition by T cells. To determine the ultrastructural cellular distribution of Ia molecules in the autoimmune disease model acute experimental allergic encephalomyelitis (EAE) we studied central nervous system (CNS) tissues from adult Strain 13 guinea pigs (GP). Experimental allergic encephalomyelitis was induced by sensitization with GP spinal cord homogenate in complete Freund's adjuvant (CFA). Nine of 11 sensitized GP had clinical and histologic EAE whereas unsensitized and CFA-sensitized controls were normal. Central nervous system tissues were reacted with monoclonal antibodies to either GP Ia or T cell surface antigen using an avidin-biotin immunoperoxidase technique and studied by electron microscopy; Ia was found on luminal but not abluminal surfaces of many meningeal and parenchymal vascular endothelial cells in GP with EAE. In EAE perivascular lymphocytes and macrophages and processes of unidentified cells in the parenchyma expressed surface Ia and Ia+ macrophages encircled and phagocytosed myelin. T cells were found predominantly in perivascular inflammatory cuffs. These observations indicate that following immunologic challenge Ia is expressed on luminal surfaces of vascular endothelium and on resident CNS cells, suggesting the possibility that these cells may have active antigen-presenting functions in CNS inflammatory reactions.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002196:Capillaries / Q000276:immunology / Q000648:ultrastructure; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D004727:Endothelium / Q000276:immunology / Q000648:ultrastructure; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007158:Immunologic Techniques; D008264:Macrophages / Q000276:immunology; D008285:Major Histocompatibility Complex; D008578:Meninges / Q000098:blood supply* / Q000276:immunology; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000276:immunology*; D014699:Venules / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II