Crystallization and preliminary x-ray diffraction studies of subtilisin GX from Bacillus sp. GX6644.
Subtilisin GX, a serine protease from Bacillus species GX6644, has been crystallized by the vapor diffusion method using ammonium sulfate as the precipitant. The space group is P212121 with a = 38.4 A, b = 70.3 A, c = 73.5 A, and one molecule in the asymmetric unit. The crystals diffract to beyond 2.0-A resolution and are suitable for a high resolution three-dimensional structure determination. All x-ray data used in the preliminary crystallographic study were collected with an electronic area detector.
D000645:Ammonium Sulfate; D001407:Bacillus / Q000201:enzymology*; D011232:Chemical Precipitation; D003460:Crystallization; D004058:Diffusion; D013381:Subtilisins; D014961:X-Ray Diffraction
D013381:Subtilisins; D000645:Ammonium Sulfate