A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.
D003208:Concanavalin A / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007898:Leishmaniasis, Visceral / Q000097:blood*; D009000:Monocytes / Q000378:metabolism*; D011485:Protein Binding
D003208:Concanavalin A