The structure of the basement membrane of human lymph node high endothelial venules: an ultrastructural, histochemical and immunocytochemical study.
The structure of the basement membrane of the high endothelium of reactive human lymph nodes was investigated by techniques selective for carbohydrates (periodic acid-Schiff; critical electrolyte concentration staining with Alcian Blue; lectin histochemistry), specific proteins (immunohistochemistry for laminin and fibronectin) and by conventional techniques of light and transmission electron microscopy. Adjacent small lymphocytes were assigned to B and T cell subsets by use of monoclonal antibodies and they were analysed for non-specific esterase, beta-glucuronidase, beta-N-acetylglucaminidase and proteolytic activities. The basement membranes were shown to be distinctive and to contain three layers, of differing laminin, glycosaminoglycan and glycoprotein oligosaccharide content. Certain lymphocytes (probably T) contained enzymes potentially able to degrade some components of these basement membranes.
D001485:Basement Membrane / Q000032:analysis / Q000648:ultrastructure; D001692:Biological Transport; D004727:Endothelium / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000648:ultrastructure; D008208:Lymphatic System / Q000648:ultrastructure*; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism