Serodiagnosis of fascioliasis in buffaloes by immunoprecipitation using adult Fasciola gigantica antigens.
Partial purification of a saline extract of adult Fasciola gigantica by chromatography on Sephadex G200 gave four peaks, of which only the first two contained material which gave a precipitation reaction with sera from infected buffaloes. None of these gave false reactions with sera from uninfected animals but even the first and most sensitive fraction only reacted with 57.5 per cent of sera from animals known to be infected with moderate numbers of F gigantica.
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002020:Buffaloes; D002850:Chromatography, Gel; D005188:False Negative Reactions; D005209:Fasciola / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D007158:Immunologic Techniques; D011233:Precipitin Tests; D011237:Predictive Value of Tests
D000947:Antigens, Helminth