title: Insulin inhibits specific norepinephrine uptake in neuronal cultures from rat brain.
abstract: Neuronal cells in primary culture have been demonstrated to possess specific insulin receptors (Boyd et al., J. Biol. Chem., 260 (1985) 15880-15884). Incubation of these cultures with insulin causes a dose-dependent inhibition of maprotiline-sensitive [3H]norepinephrine uptake. Maximum inhibition of 95% of maprotiline-sensitive norepinephrine uptake was observed at an insulin concentration of 167 nM with an ED50 of 30 nM. Competition-inhibition and Scatchard analysis of the insulin binding data suggested that maprotiline competed for high-affinity insulin receptors. These observations suggest that both insulin and maprotiline specifically inhibit neuronal norepinephrine uptake possibly involving insulin receptors.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D007328:Insulin / Q000494:pharmacology*; D008376:Maprotiline / Q000494:pharmacology; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism
chemical_list: D007328:Insulin; D008376:Maprotiline; D011972:Receptor, Insulin; D009638:Norepinephrine