title: Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae.
abstract: Deletions of a tyrosine tRNA suppressor gene, SUP4-o, are mediated by recombination between short repeated delta sequences in Saccharomyces cerevisiae. The arrangement of the five solo delta sequences that surround the SUP4 locus was established by DNA sequence analysis. Seven deletion classes were identified by genomic blotting. DNA sequence analysis also showed that the delta sequences within a 6.5-kilobase region of the SUP4 locus were the endpoints of these events. In three of these classes, an adjacent interval surrounded by delta sequences was inverted in concert with the deletion. The frequency of all deletion classes decreased in strains that contained mutations in the recombination and repair gene RAD52. We present two gene conversion mechanisms by which these rearrangements could have been generated. These models may also explain deletions between repeated sequences in other systems.
mesh_terms: D001483:Base Sequence; D002872:Chromosome Deletion; D004271:DNA, Fungal / Q000235:genetics; D005785:Gene Conversion; D005800:Genes, Fungal; D008938:Mitosis; D008957:Models, Genetic; D009154:Mutation; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D004271:DNA, Fungal; D012346:RNA, Transfer, Amino Acyl