Isolation and characterization of the receptor on human neutrophils that mediates cellular adherence.
The receptor on human neutrophils (polymorphonuclear leukocytes or PMN) that mediates cellular adherence has been purified from the peripheral blood PMN obtained from an individual with chronic myelogenous leukemia (CML). This receptor consists of two noncovalently associated subunits, designated alpha M (Mac-1 alpha, CD11b) (Mr = 170,000) and beta (Mac-1 beta, CDw18) (Mr = 100,000), respectively, which are identical on normal and CML PMN. The subunits were purified by monoclonal antibody 60.1-Sepharose (anti-alpha M) affinity chromatography and separated in 5-nmol quantities by high pressure liquid chromatography on a TSK-4000 gel filtration column. Subunits were characterized by amino acid composition, NH2-terminal amino acid sequence, and carbohydrate content. The NH2-terminal sequence of the human PMN alpha M subunit contains regions of homology with the human platelet glycoprotein IIb alpha. We conclude that nanomole amounts of individual alpha M and beta subunits of the receptor on human PMN that mediates cellular adherence can be isolated and separated using CML PMN.
D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000302:isolation & purification* / Q000502:physiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002460:Cell Line; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood*; D015470:Leukemia, Myeloid, Acute; D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; D016177:Macrophage-1 Antigen; D008970:Molecular Weight; D009504:Neutrophils / Q000502:physiology*; D011946:Receptors, Antigen / Q000302:isolation & purification* / Q000502:physiology
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; D016177:Macrophage-1 Antigen; D011946:Receptors, Antigen