Insulin or insulin-like peptides in the human pituitary.
Human pituitary cells and pancreatic islet beta cells from surgical and autopsy material showed positive immunoreactivity to anti-porcine insulin antisera demonstrating the presence in these tissues of insulin or insulin-like immunoreactive material. Glutaraldehyde-fixed, one micron epoxy sections were used and the polymerized resin was removed prior to staining with the peroxidase anti-peroxidase technique using guinea pig antisera to porcine insulin. Pancreatic beta cells served as the positive control, and appropriate negative controls were also utilized.
D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000032:analysis; D010903:Pituitary Gland, Anterior / Q000032:analysis*
D007328:Insulin