High frequency antigens of human erythrocyte membrane sialoglycoproteins, III. Studies on the EnaFR, Wrb and Wra antigens.
The nature of the common erythrocyte antigens EnaFR and Wrb, that are both absent from En(a-) cells, and the rare Wra receptor, apparently encoded by an allele of Wrb, was investigated. Various modification, fractionation or cleavage products of erythrocyte membranes were used in hemagglutination inhibition assays. The EnaFR and Wrb antigens were shown to represent labile structures within the residues approx. 62-72 of the major (MN) sialoglycoprotein that require lipids, at least for complete expression of antigenic activity. During the course of these experiments, the arrangement of the MN glycoprotein's peptide chain with respect to the lipid bi-layer was also studied, using various proteinases. Furthermore, the MN glycoprotein was found to aggregate with the major membrane protein (band 3) in the presence of Triton X-100. The Wra antigen was shown to exhibit properties that differ considerably from those of the Wrb receptor. Analyses on the MN glycoprotein, isolated from the red cells of the only known Wra homozygote and two WraWrb individuals, did not reveal any amino-acid exchange within the residues 40-96 of the molecule. Therefore, the Wr locus that determines the presence or absence of the Wrb antigen on the MN glycoprotein might influence the post-translational modification of amino-acid residues, the structure of tightly bound lipids or the aggregation of the MN glycoprotein with a different protein such as band 3.
D001789:Blood Group Antigens / Q000235:genetics / Q000276:immunology*; D010450:Endopeptidases; D004910:Erythrocyte Membrane / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007519:Isoantigens / Q000235:genetics / Q000302:isolation & purification*; D011499:Protein Processing, Post-Translational; D012795:Sialoglycoproteins / Q000097:blood / Q000235:genetics / Q000276:immunology*; D012995:Solubility
D001789:Blood Group Antigens; D007519:Isoantigens; D012795:Sialoglycoproteins; D010450:Endopeptidases