Relationship between immune system and gram-negative bacteria, binding of Salmonella pullorum-gallinarum to chicken lymphocytes.
Salmonella pullorum-gallinarum binding to chicken lymphocytes has been evaluated in individuals of different age (1-20 wks). Bacterial adherence has been studied on lymphoid cells recovered from thymus, spleen, bursa and peripheral blood. Binding was age-dependent and neuraminidase treatment of lymphoid cells led to an increase of adherence. In addition, by using two monoclonal antibodies, the CT-1, which defines thymocytes and the M-4 which identifies surface IgM and an avian homologue of mammalian IgD on B lymphocytes, distribution of Salmonella pullorum-gallinarum binding to T and B lymphocytes has been analyzed. Results show that either T lymphocytes or B lymphocytes have the capacity to form rosettes with Salmonella. In particular, B cells from bursa and spleen display the highest capacity of adherence to bacteria, this supporting the defence function which has been attributed to bursa of Fabricius.
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001422:Bacterial Adhesion; D002060:Bursa of Fabricius / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D009439:Neuraminidase / Q000494:pharmacology; D012397:Rosette Formation; D012475:Salmonella / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology
D000911:Antibodies, Monoclonal; D009439:Neuraminidase