A colorimetric method for measuring the candidacidal activity of leucocytes.
The candidacidal activity of mouse neutrophils was determined by a colorimetric method, using a tetrazolium dye that detects living, but not dead, yeast cells. The assay is carried out in microtitre trays, read using a multiwell scanning spectrophotometer (ELISA reader), and can be analysed by computer.
D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D003124:Colorimetry; D003470:Culture Media; D005260:Female; D051379:Mice; D009504:Neutrophils / Q000276:immunology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D003470:Culture Media; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue