[Postradiation activation of proteinases associated with the hepatocyte nuclear matrix in rats].
Proteinase activity of the nuclear matrix of rat hepatocytes was 7-8-times as high as that of initial nuclei. Activity of nuclear matrix proteinases was optimum at pH 8-9. Proteolytic activity associated with the nuclear matrix, increased by 1.4-2.8 times 2 h following irradiation with doses from 5 to 30 Gy. Cycloheximide, a protein synthesis inhibitor, administered to animals failed to suppress the radiation-induced increase of proteinase activity of the nuclear matrix.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D003513:Cycloheximide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D010450:Endopeptidases / Q000378:metabolism / Q000528:radiation effects*; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D008297:Male; D051381:Rats; D013997:Time Factors
D003513:Cycloheximide; D010450:Endopeptidases
Postradiatsionnaia aktivatsiia proteinaz, assotsiirovannykh s iadernym matriksom gepatotsitov krys.