An intrinsic membrane glycoprotein of the lens.
A major glycoprotein of relative molecular weight of 130,000 daltons of chick lens cell membranes was identified by Concanavalin-A staining and enriched for by Con A-Sepharose chromatography. Using both Con A binding and immunological analyses with a specific polyclonal antibody, the glycoprotein was detected in chick lens epithelium, annular pad, cortex and nucleus. Localization to the plasma membranes of lens cells was demonstrated by immunofluorescence. A similar protein, which cross-reacts with the antibody to the chick protein and also binds Con A was demonstrated in human and bovine lenses.
D000818:Animals; D002417:Cattle; D002645:Chickens; D003208:Concanavalin A; D003459:Crystallins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D007231:Infant, Newborn; D007908:Lens, Crystalline / Q000378:metabolism* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000378:metabolism*
D003459:Crystallins; D006023:Glycoproteins; D008565:Membrane Proteins; D003208:Concanavalin A