Expression of synthetic suppressor tRNA genes under the control of a synthetic promoter.
A synthetic promoter derived from the Escherichia coli lipoprotein promoter sequence was used to express synthetic suppressor tRNA genes in E. coli. These constructs, cloned into a plasmid of the pEMBL family, gave very high yields of suppressor tRNA in vivo.
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012343:RNA, Transfer / Q000235:genetics*; D013489:Suppression, Genetic
D012343:RNA, Transfer