Use of mucin and hemoglobin in experimental murine gram-negative bacteremia enhances the immunoprotective action of antibodies reactive with the lipopolysaccharide core region.
An antiserum with a high content of antibodies, binding to the Gram-negative lipopolysaccharide core region, was prepared by immunizing rabbits with the rough Escherichia coli mutant J5. This antiserum was capable of protecting mice against lethal challenge doses of E. coli 0 111:B4 in a mouse model where the animals were compromised by means of mucin plus hemoglobin (LD 50 = 10(3) bacteria). However, no protection was observed in a non-compromised mouse model (LD 50 = 10(7) bacteria). This observation might explain why in the past so many discrepant results have been obtained in mouse protection studies with cross-reactive antisera.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D003429:Cross Reactions; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology*; D005260:Female; D006454:Hemoglobins / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D009077:Mucins / Q000276:immunology*; D018805:Sepsis / Q000276:immunology
D000907:Antibodies, Bacterial; D006454:Hemoglobins; D007106:Immune Sera; D008070:Lipopolysaccharides; D009077:Mucins