Evaluation of a new confirmatory assay for antibodies against lymphadenopathy-associated virus (LAV)/human T-lymphotropic virus type III (HTLV III).
We have evaluated a newly developed competitive enzyme immunoassay for the specific detection of antibodies against LAV/HTLV III envelope and core proteins. Comparative studies of sera from high-risk donors showed complete concordance between the results of the confirmatory enzyme immunoassay and immunoblot or indirect immunofluorescence techniques. Studies of sera from blood donors with a repeatedly reactive screening test for anti-LAV/HTLV III demonstrated two specimens which were positive by the confirmatory enzyme immunoassay and negative or indeterminable by immunoblotting and immunofluorescence.
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D000914:Antibodies, Viral / Q000032:analysis*; D001782:Blood Donors; D005069:Evaluation Studies as Topic; D005260:Female; D006678:HIV / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male
D000914:Antibodies, Viral