Isolation and spectral characteristics of four toxins from the dinoflagellate Ptychodiscus brevis.
Four ichthyotoxins were isolated from crude toxic extracts of Ptychodiscus brevis using a combination of solvent partitioning, thin layer chromatography and reversed phase high pressure liquid chromatography. The toxins were analyzed by mass, infrared, 1H- and 13C-NMR spectroscopy and were found to constitute two structural families of two toxins each: brevetoxins 1 and 2 and brevetoxins 3 and 4. Comparison with literature data indicates that brevetoxins 1 and 2 are identical to the previously described and characterized 11-ring polyether toxins brevetoxins C and B, respectively. The other two compounds (brevetoxins 3 and 4) also represent a structural pair (with chloroacetone and alpha-methylene-propanal side chains, respectively) which has a different, but related, basic ring-structure.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004141:Dinoflagellida / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008387:Marine Toxins / Q000302:isolation & purification* / Q000633:toxicity; D013058:Mass Spectrometry; D011036:Poecilia; D013055:Spectrophotometry, Infrared
D008387:Marine Toxins