Correlation of the results of IFAT-DASS and ELISA tests in experimental cysticercosis of mice.
The sera of mice experimentally infected with Taenia crassiceps were tested for the occurrence of the antibodies by the enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence using defined antigen substrate spheres (IFAT-DASS). Results of both tests were compared. From day 11 p.i. until the end of the experiment (day 108 p.i.) antibodies were detected by both tests. The maximal intensity of the ELISA and IFAT-DASS reactions was observed between days 63 and 94 and days 14 and 46 p.i., respectively. ELISA is an easy-to-do and objectively appraisable method, IFAT-DASS is more suitable for testing antigens weakly adsorbing to polystyrene plates.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000947:Antigens, Helminth; D003551:Cysticercosis / Q000175:diagnosis* / Q000276:immunology; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013621:Taenia / Q000276:immunology*
D000906:Antibodies; D000947:Antigens, Helminth