Embryonal rhabdomyosarcoma in nude mice and in vitro.
A transplantable tumor strain designated YNnu and a cultured cell line designated YN were established from the human paratesticular rhabdomyosarcoma of a 15-year-old boy. In vitro the cells showed ultrastructural features of immature mesenchymal cells, and a few cells were labeled by antibodies to myoglobin and/or desmin. In nude mice, the cells became to contain numerous myofibrils with Z-bands, and considerable number of cells reacted with anti-myoglobin and/or anti-desmin antibodies. We conclude that spindle-shaped or small round cells are the most immature rhabdomyoblast with multiplicity for cellular differentiation.
D000293:Adolescent; D000818:Animals; D002460:Cell Line; D003893:Desmin / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008854:Microscopy, Electron; D009211:Myoglobin / Q000032:analysis; D009368:Neoplasm Transplantation; D012208:Rhabdomyosarcoma / Q000032:analysis / Q000473:pathology* / Q000648:ultrastructure; D013736:Testicular Neoplasms / Q000032:analysis / Q000473:pathology* / Q000648:ultrastructure
D003893:Desmin; D009211:Myoglobin