Antibody chimera technique applied to the detection of Escherichia coli heat-labile enterotoxin.
Covalently prepared chimera antibodies were tested in a ganglioside GM1 erythro-immunoassay (CERIA) for E. coli heat-labile enterotoxin (LT) detection. The antibody specific for LT was conjugated with a polyclonal antibody specific for sheep erythrocytes. The assay is based on the specific binding of LT to polystyrene-adsorbed GM1 and subsequent erythro-adsorption via chimera antibody by which the bound toxin is visualized. Enterotoxin titers determined with this CERIA method were similar to those obtained with the Vero cell assay and with ELISA. 5 ng of cholera toxin/ml may be detected with the assay. The CERIA, as described, may be used either qualitatively or quantitatively and is well suited for routine laboratory diagnosis of LT in a culture supernatant of E. coli.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002678:Chimera; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology*; D006358:Hot Temperature; D007118:Immunoassay / Q000379:methods*; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000276:immunology; D012756:Sheep
D000907:Antibodies, Bacterial; D004768:Enterotoxins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C034993:ganglioside receptor