Effect of heat on endotoxin in plasma and in pyrogen-free water, as measured in the Limulus amoebocyte lysate assay.
Four modes of heating endotoxin in plasma and two different times of heating endotoxin in pyrogen-free water were compared. There were no significant differences in standard curves after heating endotoxin in plasma at 100 degrees C for 1 and for 10 min. However, the standard curve after heating for 10 min at 75 degrees C had a significantly less steep slope, and after heating for 10 min at 56 degrees C, it was completely flat. Heating of endotoxin in pyrogen-free water for 1 min also resulted in the flattening of the standard curve, which was even more pronounced after 10 min of heating.
D004731:Endotoxins / Q000032:analysis / Q000097:blood*; D004926:Escherichia coli; D006358:Hot Temperature; D006801:Humans; D008033:Limulus Test; D011749:Pyrogens; D013997:Time Factors; D014867:Water / Q000032:analysis
D004731:Endotoxins; D011749:Pyrogens; D014867:Water; C044101:endotoxin, Escherichia coli