Biological role of DNA methylation: sequence-specific single-strand breaks associated with hypomethylation of GATC sites in Escherichia coli DNA.
The effect of methylation of GATC sites in Escherichia coli DNA on the formation of single-strand breaks was studied with dam+, dam mutant, and Dam-overproducer strains. Single-strand breaks have been observed in dam mutant cells predominantly at TpT and, to a lesser extent, at CpC. In dam mutant cells harboring pTP166 (a plasmid containing the dam gene), no such nicks were observed.
D001483:Base Sequence; D004249:DNA Damage; D004269:DNA, Bacterial / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000502:physiology*; D010957:Plasmids; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)