title: Crystallization and preliminary X-ray investigation of uridine phosphorylase from Escherichia coli.
abstract: Crystals of uridine phosphorylase from Escherichia coli K12 have been grown from solutions of polyethylene glycol 4000. The crystals are trigonal, space group R3; the hexagonal axes are a = 154.4 A and c = 49.4 A. The crystals are quite stable to x-rays and diffract beyond 2.6 A resolution. It appears that the molecule is a hexamer with a subunit molecular weight of 27,500 and utilizes the 3-fold symmetry of the space group, resulting in two subunits/asymmetric unit.
mesh_terms: D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D010430:Pentosyltransferases; D014543:Uridine Phosphorylase; D014961:X-Ray Diffraction
chemical_list: D010430:Pentosyltransferases; D014543:Uridine Phosphorylase