Fluorescence-activated cell analysis of P-fimbriae receptor accessibility on uroepithelial cells of patients with renal scarring.
Pyelonephritogenic Escherichia coli possess P-fimbriae that bind to uroepithelial cells by recognizing a receptor containing the alpha-D-Galp-(1-4)-beta-D-Galp carbohydrate structure. The accessibility of P-fimbriae receptors was determined using uroepithelial cells from 19 female patients with verified renal scarring and a history of febrile urinary tract infection, and 13 healthy controls. The binding of fluorescein isothiocyanate labeled P-fimbriated Escherichia coli to a large number of uroepithelial cells from each individual was studied using the method of fluorescence-activated cell sorting analysis, which allows examination of a large number of cells and selection of cells of a given size. The uroepithelial cells from the patients exhibited a significantly higher binding capacity to P-fimbriated Escherichia coli than did uroepithelial cells from healthy controls (p less than 0.01). The determination of P-fimbriae receptor accessibility on uroepithelial cells may be useful for detecting risk groups among patients with recurrent urinary tract infections.
D000328:Adult; D002469:Cell Separation; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000382:microbiology; D004912:Erythrocytes / Q000378:metabolism / Q000382:microbiology; D004926:Escherichia coli / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007668:Kidney / Q000473:pathology; D008875:Middle Aged; D011704:Pyelonephritis / Q000378:metabolism / Q000473:pathology*; D011971:Receptors, Immunologic / Q000032:analysis* / Q000378:metabolism; D014551:Urinary Tract / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D014552:Urinary Tract Infections / Q000382:microbiology; D014556:Urine / Q000166:cytology
D011971:Receptors, Immunologic; C037872:pili, bacterial receptor