title: Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli.
abstract: Heat and various inhibitory chemicals were tested in Escherichia coli for the ability to cause accumulation of adenylylated nucleotides and to induce proteins of the heat shock (htpR-controlled), the oxidation stress (oxyR-controlled), and the SOS (lexA-controlled) regulons. Under the conditions used, heat and ethanol initiated solely a heat shock response, hydrogen peroxide and 6-amino-7-chloro-5,8-dioxoquinoline (ACDQ) induced primarily an oxidation stress response and secondarily an SOS response, nalidixic acid and puromycin induced primarily an SOS and secondarily a heat shock response, isoleucine restriction induced a poor heat shock response, and CdCl2 strongly induced all three stress responses. ACDQ, CdCl2, and H2O2 each stimulated the synthesis of approximately 35 proteins by factors of 5- to 50-fold, and the heat shock, oxidation stress, and SOS regulons constituted a minor fraction of the overall cellular response. The pattern of accumulation of adenylylated nucleotides during these treatments was inconsistent with a simple role for these nucleotides as alarmones sufficient for triggering the heat shock response, but was consistent with a role in the oxyR-mediated response.
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology; D000634:Aminoquinolines / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006150:Guanine Nucleotides / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009268:Nalidixic Acid / Q000494:pharmacology; D009711:Nucleotides / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology
chemical_list: D000227:Adenine Nucleotides; D000634:Aminoquinolines; D006150:Guanine Nucleotides; D006360:Heat-Shock Proteins; D009711:Nucleotides; D002104:Cadmium; C018817:6-amino-7-chloro-5,8-dioxoquinoline; D009268:Nalidixic Acid; D000431:Ethanol; D011691:Puromycin; D006861:Hydrogen Peroxide; D019256:Cadmium Chloride