Anti-platelet opsonic activity in alloimmune and autoimmune thrombocytopenia.
A chemiluminescence technique (CLT) has been developed which measures the interaction between human monocytes and antibody-coated (opsonized) platelets. This technique has an objective end-point, is simple to perform and is of comparable sensitivity to the platelet suspension immunofluorescence test (PSIFT) when used to detect anti-platelet allo-antibodies. In contrast, only 4/20 sera from patients with clinically diagnosed autoimmune thrombocytopenia were opsonic in the CLT, while 8/20 of these same sera bound IgG to platelets in the PSIFT. Only one serum gave positive results in both tests.
D000906:Antibodies / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000032:analysis* / Q000276:immunology; D008163:Luminescent Measurements; D008165:Luminol; D008722:Methods; D009000:Monocytes / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis; D013921:Thrombocytopenia / Q000097:blood / Q000276:immunology*
D000906:Antibodies; D006680:HLA Antigens; D007518:Isoantibodies; D009895:Opsonin Proteins; D008165:Luminol