Radioimmunoassay in blood plasma of arginine esterase: the major secretory product of dog prostate.
We have developed a radioimmunoassay to determine serum levels of arginine esterase, the major secretory product of the canine prostate. Although the antibodies recognized other related proteins in immunoblotting conditions, particularly in the pancreas, the radioimmunoassay appeared specific for arginine esterase of prostatic origin and reacted only slightly or not at all with cytosolic proteins from salivary glands, liver, skeletal muscles, and kidney. In serum samples, the sensitivity limit of the assay was about 2.5 ng per ml. The interassay and intraassay variations for serum samples that contained concentrations of arginine esterase ranging from 6 to 21 ng per ml were, respectively, 14% and 24%. Sera from 30 adult males and 14 adult females contained, respectively, 71 +/- 9 and 5.3 +/- 0.4 ng per ml. In a group of 12 dogs treated daily for 36-39 days with 50 micrograms of D-Trp6-luteinizing hormone-releasing hormone ethylamide, the concentrations of arginine esterase in the serum decreased progressively and became identical with those observed in the females. These results constitute the first evidence for the presence of a serum marker of the normal prostate in an experimental model. It should be particularly useful to test the effects of various agents on the prostate.
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D003429:Cross Reactions; D004285:Dogs / Q000097:blood*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives; D007163:Immunosorbent Techniques; D008297:Male; D011467:Prostate / Q000201:enzymology / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods; D012661:Semen / Q000032:analysis; D013739:Testosterone / Q000097:blood; D017329:Triptorelin Pamoate / Q000031:analogs & derivatives*
D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; C019176:Tryptal; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases