Localization of inclusion bodies in Escherichia coli overproducing beta-lactamase or alkaline phosphatase.
High-level synthesis of the periplasmic protein beta-lactamase in Escherichia coli caused the formation of insoluble protein precipitates called inclusion bodies. beta-Lactamase inclusion bodies differed from those reported previously in that they appeared to be localized in the periplasmic space, not in the cytoplasm. The inclusion bodies contained mature beta-lactamase and were solubilized more easily than has been reported for cytoplasmic inclusion bodies. In contrast, overproduction of the periplasmic protein alkaline phosphatase caused the formation of cytoplasmic inclusion bodies containing alkaline phosphatase precursor.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D003593:Cytoplasm / Q000032:analysis / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D001618:beta-Lactamases / Q000096:biosynthesis*
D000469:Alkaline Phosphatase; D001618:beta-Lactamases