DNA sequence preferences for an intercalating porphyrin compound revealed by footprinting.
The DNA sequence preferences of the compound meso-tetra-(4-N-methyl(pyridyl) porphyrin and its nickel complex have been investigated by means of footprinting experiments on several DNA fragments, using DNAase I and micrococcal nuclease as footprinting agents. A complex pattern of both AT and GC-protected sites was found. Ligand-induced long-range conformational changes were inferred in several instances to be related to the observed large-scale blockages of enzymatic cutting.
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D007364:Intercalating Agents; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D011166:Porphyrins
D007364:Intercalating Agents; D011166:Porphyrins; C021096:tetra(4-N-methylpyridyl)porphine; D004247:DNA