The complete amino acid sequence of chicken skeletal-muscle enolase.
The complete amino acid sequence of chicken skeletal-muscle enolase, comprising 433 residues, was determined. The sequence was deduced by automated sequencing of hydroxylamine-cleavage, CNBr-cleavage, o-iodosobenzoic acid-cleavage, clostripain-digest and staphylococcal-proteinase-digest fragments. The presence of several acid-labile peptide bonds and the tenacious aggregation of most CNBr-cleavage fragments meant that a commonly used sequencing strategy involving initial CNBr cleavage was unproductive. Cleavage at the single Asn-Gly peptide bond with hydroxylamine proved to be particularly useful. Comparison of the sequence of chicken enolase with the two yeast enolase isoenzyme sequences shows that the enzyme is strongly conserved, with 60% of the residues identical. The histidine and arginine residues implicated as being important for the activity of yeast enolase are conserved in the chicken enzyme. Secondary-structure predictions are analysed in an accompanying paper [Sawyer, Fothergill-Gilmore & Russell (1986) Biochem. J. 236, 127-130].
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D019811:Hydroxylamine; D006898:Hydroxylamines; D007463:Iodobenzoates; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000302:isolation & purification*
D006898:Hydroxylamines; D007463:Iodobenzoates; D010446:Peptide Fragments; D019811:Hydroxylamine; C008154:2-iodosobenzoic acid; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C019913:clostripain; D010751:Phosphopyruvate Hydratase; D003488:Cyanogen Bromide