[Isolation and fractionation of the nuclear matrix from cultured cells].
A new method for the isolation of tissue culture cell nuclei is presented which involves incubation of the nuclei in the presence of Cu2+- or Zn2+-ions. This method eliminates the danger of nuclear aggregation and permits nuclear matrix isolation and subsequent fractionation. Stabilization of the inner matrix by Cu2-ions permits analysis of the role of nucleic acids in the maintenance of the matrix structure. It is shown that solubilization of more than 95% of matrix-bound DNA and more than 90% of matrix-bound RNA did not cause any significant changes in the nuclear matrix structure.
D000818:Animals; D002449:Cell Aggregation; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000302:isolation & purification; D051379:Mice; D010954:Plasmacytoma / Q000648:ultrastructure; D012334:RNA, Neoplasm / Q000302:isolation & purification
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm
Vydelenie i fraktsionirovanie iadernogo matriksa iz kul'tiviruemykh kletok.