title: Properties of glucose uptake in vegetative and sporulating cells of Saccharomyces cerevisiae.
abstract: The effect of digitonin, acetic acid, urea and ethanol treatment on the glucose uptake of vegetative cells and of sporulating cells (3 h after transfer to sporulation medium) was examined in Saccharomyces cerevisiae. Both glucose uptake activities decreased at a similar rate, and a slightly different rate, in treatment with various concentrations of digitonin and of acetic acid, respectively, at 25 degrees C for 10 min. The glucose uptake activity of the sporulating cells was much more stable to urea treatment than that of the vegetative cells; the activity decreased about 36% and 76% in the sporulating cells and the vegetative cells, respectively, under conditions of 2.5 M urea at 25 degrees C for 10 min. The glucose uptake activity of the vegetative cells was more stable to ethanol treatment than that of the sporulating cells; the activity decreased about 56% and 88% in the vegetative cells and the sporulating cells, respectively, in 25% ethanol at 25 degrees C for 10 min.
mesh_terms: D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D004072:Digitonin / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013172:Spores, Fungal / Q000187:drug effects / Q000378:metabolism; D014508:Urea / Q000494:pharmacology
chemical_list: D000085:Acetates; D000431:Ethanol; D014508:Urea; D005947:Glucose; D004072:Digitonin; D019342:Acetic Acid