Influence of insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA) on influenza virus multiplication.
Insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA) interfere with the multiplication of fowl plague virus, an influenza A virus, in primary chick embryo cells. Specifically the production of the viral glycoproteins hemagglutinin and neuraminidase are affected by the drugs. A decrease or omission of glucose from the culture medium enhances this effect, which is in agreement with the idea that these drugs act on virus replication via a shortage of glucose in the host cell. Virus replication in cells of different organs is affected to different extents by insulin and TPA.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism*; D009980:Influenza A virus / Q000187:drug effects* / Q000502:physiology; D007328:Insulin / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D007328:Insulin; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate