title: [Optimization of the immunofluorescence test for antibodies in the serum of patients with inner ear diseases].
abstract: Antibodies in the serum of patients with inner ear diseases can be demonstrated immunohistochemically with human kidney tissue. In this study a patient's serum with the typical positive reaction against proximal tubuli (antibodies against microsomes) and a serum with antibodies against mitochondria was tested with different dilutions of the fluorescein marked antiserum (FITC conjugate). For both sera a dilution of 1:160 showed positive reaction. The fluorescence of the control incubation was negligible. Employing the given technical details the immunohistochemical assay of antibodies with human kidney tissue has become a simple and reliable test for the most frequently found antibodies in patients with inner ear diseases.
mesh_terms: D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology*; D007758:Ear, Inner / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006319:Hearing Loss, Sensorineural / Q000276:immunology; D006801:Humans; D007759:Labyrinth Diseases / Q000276:immunology*; D008861:Microsomes / Q000276:immunology; D008928:Mitochondria / Q000276:immunology
chemical_list: D001323:Autoantibodies
vernacular_title: Optimierung des Immunfluoreszenztests für Antikörper im Serum von Patienten mit Innenohrerkrankungen.