Determination of L-buthionine-(S,R)-sulfoximine in plasma by high-performance liquid chromatography with o-phthalaldehyde derivatization and fluorometric detection.
The development of a high-performance liquid chromatography system for the analysis of L-buthionine-(S,R)-sulfoximine (BSO) in human plasma is described. o-Phthalaldehyde derivatization and fluorescence detection were used. The R- and S-BSO peaks were partially separated from each other and completely separated from the matrix components. The limit of detection for BSO was 2 micrograms/ml plasma.
D019328:Buthionine Sulfoximine; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives* / Q000097:blood; D013050:Spectrometry, Fluorescence; D009764:o-Phthalaldehyde
D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D009764:o-Phthalaldehyde