[Immunological properties of isepamicin (HAPA-B)].
Immunological properties of isepamicin (HAPA-B), a new semisynthetic aminoglycoside, were compared with those of gentamicin (GM). The results are summarized as follows. Rabbits were immunized by HAPA-B or GM with a strong adjuvant, Freund's complete adjuvant, and specific IgG antibodies were found in sera when assayed using PHA and PCA reactions. In sera from guinea pigs and mice immunized by HAPA-B or GM with aluminum hydroxide gel, no specific IgG nor IgE antibody was detected when assayed using PCA reactions. Specific antibodies (IgG, IgE) produced against HAPA-B and GM were confirmed in different animals immunized by chemically introduced antigens of HAPA-B-carrier protein conjugate and GM-carrier protein conjugate, respectively. Guinea pigs immunized by HAPA-B or HAPA-B-carrier protein conjugate with aluminum hydroxide gel did not exhibit typical systemic anaphylactic reactions when elicited with HAPA-B alone. HAPA-B had some immunocross-reactivity with GM. These results suggested that the antibody-producing activity of HAPA-B was similar to that of GM.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D003429:Cross Reactions; D005839:Gentamicins / Q000276:immunology*; D006168:Guinea Pigs; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011817:Rabbits
D005839:Gentamicins; D007074:Immunoglobulin G; D007073:Immunoglobulin E; C016927:isepamicin