Immunocytochemical localization of serotonin in intracellularly analyzed and dye-injected ganglion cells of the turtle retina.
Combining intracellular recording techniques with immunocytochemistry revealed the existence of serotonin within a few ganglion cells of the turtle retina. Lucifer yellow was intracellularly injected into physiologically classified ganglion cells. Sections containing labeled somata were subsequently incubated with an antibody directed against serotonin and immunoreactivity was revealed using horseradish peroxidase as a marker. In 3 ganglion cells the two markers were co-localized. These cells ramified in layers 2 and 3 of the inner plexiform layer and produced color opponent ON-OFF photoresponses.
D000818:Animals; D001360:Avidin; D001710:Biotin; D006651:Histocytochemistry; D007120:Immunochemistry; D007124:Immunoenzyme Techniques; D007546:Isoquinolines; D012160:Retina / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D014426:Turtles / Q000378:metabolism*
D007546:Isoquinolines; D001360:Avidin; D012701:Serotonin; D001710:Biotin; C017475:lucifer yellow