Evidence that the sigma 1 protein of reovirus serotype 3 is a multimer.
In this report, we study the reovirus serotype 3 (strain Dearing) sigma 1 protein obtained from various sources: from Escherichia coli expressing sigma 1 protein, from reovirus-infected mouse L cells, and from purified reovirions. We demonstrate that the sigma 1 protein is a multimer in its undisrupted form and present biochemical evidence suggesting that the multimer is made up of four sigma 1 subunits.
D000818:Animals; D036022:Capsid Proteins; D004926:Escherichia coli / Q000378:metabolism; D007739:L Cells / Q000378:metabolism; D012089:Mammalian orthoreovirus 3 / Q000032:analysis*; D051379:Mice; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000032:analysis*; D012087:Reoviridae / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000032:analysis
D036022:Capsid Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; C031728:sigma 1 protein, reovirus