Fixation of neural tissue for electron microscopy with an electronically controlled perfusion pump.
Many attempts to improve the perfusion of mammalian tissues aim at changes of the osmotic pressure. We describe a method for fixation of nervous tissues controlling both the hydrostatic pressure and the flow rate of a perfusion solution. The constancy of these parameters is guaranteed by an electronically controlled perfusion pump. Thus, a more uniform and complete preservation can be achieved. Further advantages of this method include provision for a rapid succession of rinsing and fixation solution and a continuous control of the hydrostatic pressure during perfusion.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D006652:Histological Techniques; D008297:Male; D008854:Microscopy, Electron; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains