Gene transfer as a novel approach to the gene-controlled mechanism of the cellular expression of glycosphingolipids.
Gene transfer is a useful technique to analyse the gene-controlled mechanism of cellular expression of glycosphingolipids. Using various oncogenes (DNA tumor virus oncogenes: adenovirus 12 E1 and its transcriptional subunits, myc and SV40 T antigens; RNA tumor retrovirus oncogens: fos, src and H-ras) for the transfer, it was demonstrated that these genes invariably caused remarkable and characteristic changes of the cellular expression of gangliosides in the same recipient rat embryonic cell line, 3Y1. As a consequence of the transfer, various ganglioside species appeared or disappeared, the extent of the changes varying according to the type of oncogenes used. Analysis of the enzymatic background of such changes was also carried out. Based on these results, the effects of oncogene transfer on glycosphingolipids were classified and discussed according to the mode of subcellular distribution of gene products.
D000818:Animals; D002460:Cell Line; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005732:Gangliosides / Q000235:genetics; D005786:Gene Expression Regulation; D006028:Glycosphingolipids / Q000235:genetics*; D009857:Oncogenes; D014162:Transfection; D014170:Transformation, Genetic
D005732:Gangliosides; D006028:Glycosphingolipids