Antibodies to the C-terminus of the cholecystokinin precursor: radioimmunoassay and immunohistochemical studies in adult and developing rat gut.
Antibodies to the extreme C-terminal pentapeptide of procholecystokinin, YEYPS (in the single letter notation), have been used in radioimmunoassay and immunohistochemistry to characterize the material in rat intestine. There is a single major immunoreactive peptide in intestinal extracts that has the properties of the C-terminal tryptic peptide of the CCK precursor. Similar material has previously been found in rat brain. In immunohistochemistry, a population of cells in rat small intestine is revealed, these also contain C-terminal gastrin/CCK activity, but for the most part do not react with gastrin-specific antibodies. During postnatal development their numbers increase considerably. We conclude that the C-terminal flanking peptide of proCCK is a useful marker for sites of CCK production.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002766:Cholecystokinin / Q000032:analysis*; D002850:Chromatography, Gel; D004064:Digestive System / Q000032:analysis* / Q000254:growth & development; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D010446:Peptide Fragments / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats
D000906:Antibodies; D010446:Peptide Fragments; C052442:cholecystokinin precursor C-terminal pentapeptide; D002766:Cholecystokinin