Demonstration of anti-mitochondria antibodies by ELISA. Comparison with the indirect immunofluorescence technique on organ sections.
We determined the optimal conditions for the detection of anti-mitochondria antibodies by an enzyme linked immunosorbent assay (ELISA). Pig heart mitochondria was used at 5 mg/ml to coat polystyrene microplates and a serum dilution of 1/200 to carry out testing. This procedure detects anti-M1, M2, M4 and M6 antibodies and is more sensitive than indirect immunofluorescence. Anti-endoplasmic reticulum and anti-ribosome antibodies give negative results but anti-ds-DNA at high titres are a possible cause of false positivity.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D002417:Cattle; D003951:Diagnostic Errors; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006521:Hepatitis, Chronic / Q000276:immunology; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000276:immunology; D051379:Mice; D008928:Mitochondria / Q000276:immunology*; D051381:Rats; D013552:Swine
D001323:Autoantibodies