Induction and inhibition of cathepsin B and hemoglobin-hydrolase activity in murine B16 melanoma by thiol protease inhibitors.
The effects of potent thiol protease inhibitors in vitro (leupeptin, antipain, chymostatin and E-64 (N-[N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucyl]agmatine) on intracellular cathepsin B and hemoglobin (Hb)-hydrolase from cultured B16 melanoma cells were studied. E-64 induced cultured B16 melanoma cells to decrease the activities of intracellular cathepsin B (EC 3.4.22.1.) but did not have this effect with Hb-hydrolase or acid phosphatase (EC 3.1.3.2). Leupeptin, antipain and chymostatin induced B16 melanoma cells to increase the activities of intracellular cathepsin B and Hb-hydrolase but not that of acid phosphatase. These results indicate that there are two kinds of thiol protease inhibitors, each with a varying reaction to cultured B16 melanoma--inhibition of intracellular cathepsin B, and conversely, inducement of both cathepsin B and Hb-hydrolase.
D000818:Animals; D000976:Antipain / Q000494:pharmacology*; D002401:Cathepsin B / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D007700:Kinetics; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology*; D008546:Melanoma, Experimental / Q000201:enzymology*; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D011480:Protease Inhibitors
D007976:Leupeptins; D009842:Oligopeptides; D011480:Protease Inhibitors; D000976:Antipain; C032425:N-(N-(3-carboxyoxirane-2-carbonyl)leucyl)isoamylamine; C002101:chymostatin; D010447:Peptide Hydrolases; C022759:hemoglobin hydrolase; D002401:Cathepsin B; D007930:Leucine; C032854:leupeptin