[The vector containing a signal for specific degradation of chimeric proteins. Synthesis of [Leu5]enkephalin using enteropeptidase].
A plasmid vector (pEK1) coding, in framework of beta-galactosidase gene, for the amino acid sequence (Asp)4Lys which is recognized by bovine enteropeptidase has been constructed. Using this vector and chemically synthesized DNA coding for the [Leu5]-enkephalin, a plasmid (pEK-ENK) has been obtained in which the beta-galactosidase gene is fused, through the enteropeptidase linker, with the gene for [Leu5]enkephalin. The chimeric protein produced by expression of this plasmid has been isolated and then cleaved by the enteropeptidase to give [Leu5]enkephalin with the yield 74%.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D010450:Endopeptidases / Q000378:metabolism*; D004743:Enkephalin, Leucine / Q000096:biosynthesis* / Q000378:metabolism; D004765:Enteropeptidase / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007413:Intestinal Mucosa / Q000201:enzymology; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism
D011994:Recombinant Proteins; D004743:Enkephalin, Leucine; D010450:Endopeptidases; D004765:Enteropeptidase
Vektor, soderzhashchiĭ signal dlia spetsificheskogo rasshchepleniia khimernykh belkov. Poluchenie [Leu5]énkefalina s pomoshch'iu énteropeptidazy.