The interaction of brain insulin receptors with wheat germ agglutinin.
Brain insulin receptors adsorb to and are recoverable from wheat germ agglutinin-agarose (WGA) columns. Similar results are obtained using dissuccinimidyl suberate (DSS)-crosslinked receptors or photo-affinity labeled receptors. WGA can be used for partial purification of brain insulin receptors provided the appropriate WGA preparation is chosen and the optimal ratio of receptor protein to lectin is achieved.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011972:Receptor, Insulin / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
C042758:wheat germ agglutinin-sepharose 6MB; D012685:Sepharose; D011972:Receptor, Insulin