[Cultivation of murine B-cell hybridomas in the spleen].
The tumorigenic capacity of mouse B-cell hybridomas in both cloned and primary cultures was studied. The cells were selected for inoculation from 24-well plates and introduced into the spleen of syngeneic mice. The cells took in 50% of the animals. The cells of hybridoma tumors induced as the result of intrasplenic inoculation, when subcultured in the second passage following the standard scheme, i.e. inoculated intraperitoneally in a dose of 1 X 10(7) cells into mice previously treated with vaseline oil or pristane, produced tumors in 100% of the animals.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology*; D003584:Cytological Techniques; D004306:Dose-Response Relationship, Immunologic; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology / Q000637:transplantation; D007114:Immunization / Q000379:methods; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology / Q000276:immunology; D013154:Spleen / Q000166:cytology*
D000911:Antibodies, Monoclonal
Kul'tivirovanie myshinykh B-kletochnykh gibridom v selezenke.