[Localization of binding sites of E. coli DNA-dependent RNA-polymerase with photosensitive template analogs].
The photoinduced covalent binding of E. coli RNA polymerase with decathymidylic templates containing 5-bromouracil residue has been carried out. Peptides from beta and beta' subunits of the core-enzyme, situated in the DNA-template binding site of the RNA polymerase active center have been localized.
D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D009841:Oligonucleotides; D010777:Photochemistry; D013379:Substrate Specificity; D013698:Templates, Genetic
D009841:Oligonucleotides; D012321:DNA-Directed RNA Polymerases
Lokalizatsiia mest sviazyvaniia DNK-zavisimoĭ RNK-polimerazy E. coli s fotochuvstvitel'nymi analogami matritsy.