Monocyte-T lymphocyte interaction for regulation of insulin receptors of the activated T lymphocyte.
During activation the lymphocyte attains functional insulin receptors with precise regulation, a consequence of insulin concentration manipulations. These studies test the hypothesis that insulin receptor (+) monocytes monitor insulin concentrations, so instructing  the T lymphocytes. Monocyte-enriched populations were incubated with insulin (0-10(-6) M) followed by co-culture with T lymphocytes and an activating stimulus. A dose-related fall in T lymphocyte insulin receptor binding was observed that was specific for the monocyte as the signalling cell and for insulin as the signal received. Monocytes from normal volunteers during a euglycemic, hyperinsulinemic clamp were cultured with T lymphocytes and an activating stimulus. A decline in specific insulin receptor binding on T lymphocytes was observed, which Scatchard analysis demonstrated to be a consequence of reduction in receptor numbers. These studies demonstrate that the receptor (+) monocyte perceives the concentration of insulin and passes this information to T lymphocytes regulating the number of activation-induced insulin receptors. The interplay between the monocyte and T lymphocyte parallels the interaction of these cell types for recognition of antigen.
D000818:Animals; D002478:Cells, Cultured; D005215:Fasting; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000097:blood* / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism*; D013154:Spleen; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology*
D007328:Insulin; D011972:Receptor, Insulin