Comparison of Clostridium difficile detection by monolayer and by inhibition of nucleoside uptake.
Detection and identification of Clostridium difficile toxin by traditional monolayer assay were compared with results obtained by a new procedure based on toxin-dependent inhibition of target cell uptake of a radioactive nucleoside. A high degree of correlation was noted between the two determinations. Although the new procedure was quantitative and objective, its value is seen at present as a rapid screen that may support results obtained in monolayers and as a potential assay for other, currently unidentified, toxins.
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D002460:Cell Line; D003013:Clostridium / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D006801:Humans; D007065:Idoxuridine / Q000378:metabolism; D007457:Iodine Radioisotopes; D007940:Leukemia L5178 / Q000378:metabolism; D051379:Mice; D009705:Nucleosides / Q000378:metabolism*
D001427:Bacterial Toxins; D007457:Iodine Radioisotopes; D009705:Nucleosides; D007065:Idoxuridine