Ontogenesis of 28 kDa vitamin D-induced calcium-binding protein in human kidney.
The kidney distribution of 28 kDa vitamin D-induced calcium binding protein (CaBP) was studied in 15 fetuses (11 to 33 weeks old), six children and adults (12 days to 32 years old) by immunocytochemistry using a specific antibody to rat renal 28 kDa CaBP. Similar results were obtained on frozen and fixed tissues. Kidneys from one adult and three fetuses were studied by immunoelectronmicroscopy for antigen localization at the subcellular level using the indirect immunoperoxidase technique. The 28 kDa CaBP was present in all kidneys from the eleventh week of gestation. At that stage, all deep parts of collecting ducts were homogeneously stained and a few distal tubules located in the deep cortex were intensely labeled. No labeling was observed in the early stage of nephron differentiation (S-body). 28 kDa CaBP distribution changed with kidney maturation. There was a progressive reduction of the deep part of collecting duct labeling and a concomitant increase in the number and intensity of stained distal tubular cells. At the ultrastructural level, 28 kDa CaBP was observed in the cytosol and the nuclear euchromatin. Our study demonstrates the early cellular synthesis of 28 kDa CaBP and its transient expression by deep collecting duct cells during early fetal life, at a time when only a few distal convoluted tubular cells synthetize it.
D000328:Adult; D000375:Aging; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D002675:Child, Preschool; D005260:Female; D005333:Fetus; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000254:growth & development*; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D011247:Pregnancy; D064030:S100 Calcium Binding Protein G / Q000032:analysis*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D064030:S100 Calcium Binding Protein G