Immunostaining of colorectal cancer with monoclonal anti-CEA antibodies compared to serum and tumor CEA content.
Carcinoembryonic antigen was measured in serum and in extracts from 37 colorectal tumors and we found a poor correlation between circulating and tumor CEA. Monoclonal antiCEA antibodies were used in indirect immunoperoxidase staining of the corresponding formalin fixed tissue sections. We found that serum CEA measurement had a sensitivity of only 41.9% as compared to 90.3% for the immunohistochemical staining. The positive and negative predictive values for immunostaining were respectively 96.6% and 72.7%. Immunohistochemical staining of tissue sections with monoclonal anti CEA coupled to other biochemical or immunological assays could be a useful adjunct for the diagnosis of premalignant or slightly modified tissues.
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000097:blood / Q000276:immunology; D003110:Colonic Neoplasms / Q000032:analysis*; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D012004:Rectal Neoplasms / Q000032:analysis*
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen