N-hydroxysuccinimide-hippuran ester: application for radiolabeling of macro-molecules.
A method for synthesis of N-hydroxysuccinimide ester of radioactive hippuran is developed in order to label human serum albumin in a simple and efficient manner. Organ distribution in mice and rats for the labeled albumin preparation and the commercial radioiodinated serum albumin is similar. Hippuran metabolite released from the labeled preparation into the blood stream results in its rapid urinary clearance. The hippuran labeling method offers a mild and rapid protocol for radioiodine labeling of proteins and antibodies for application in diagnostic nuclear medicine procedures.
D000818:Animals; D007457:Iodine Radioisotopes; D007465:Iodohippuric Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism; D013388:Succinimides / Q000138:chemical synthesis / Q000378:metabolism; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D012709:Serum Albumin; D013388:Succinimides; C052312:N-hydroxysuccinimide hippuran ester; D007465:Iodohippuric Acid