Modulation of proliferation of a human head and neck squamous carcinoma cell line (HN-1) by catecholamines and histamine.
The effects of catecholamines and histamine on the HN-1 human head and neck squamous carcinoma cell line were determined by analyzing binding of (-)125 I-iodocyanopindolol (delta-adrenergic antagonist), clonogenicity in soft agar, and cell cycle effects by dual-parameter flow cytometry. Binding studies revealed the presence of delta-adrenergic receptors with an affinity between 11-13 pM and a capacity between 5.8.-8.5 fmol/10(5) cells. A clonogenic assay demonstrated that both isoproterenol and histamine gave a minor inhibition of clonogenicity which could be reversed with propranolol and cimetidine respectively. Pretreatment with propranolol followed by isoproterenol treatment abolished the inhibitory effect of isoproterenol on clonogenicity. Catecholamines and histamine modulated the proliferation of HN-1 cells which was generated through receptor binding.
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002395:Catecholamines / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004273:DNA, Neoplasm / Q000378:metabolism; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism
D002395:Catecholamines; D004273:DNA, Neoplasm; C047975:cyanopindolol; D006632:Histamine; D010869:Pindolol