Electrorotation of lymphocytes--the influence of membrane events and nucleus.
Electrorotation--the spin of cells in rotating high frequency electric fields--has been used to investigate properties of human peripheral blood lymphocytes. The rotation spectra of lymphocytes deviate from those of single shell spheres. The deviations are caused by the electrical properties of the nucleus in the cell interior. Electrorotation allows the distinction between successfully stimulated lymphocytes and unstimulated cells after application of concanavalin A. Notwithstanding the fact that only a proportion of the cells will be mitogenically stimulated we detected an enhanced cell membrane conductivity for the whole cell population immediately after the addition of mitogen.
D002462:Cell Membrane / Q000502:physiology*; D002467:Cell Nucleus / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods*; D003208:Concanavalin A / Q000494:pharmacology; D004553:Electric Conductivity; D004574:Electromagnetic Fields; D055590:Electromagnetic Phenomena; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009685:Nuclear Envelope / Q000502:physiology* / Q000648:ultrastructure; D012399:Rotation
D003208:Concanavalin A