Production of human monoclonal antibodies from immobilized cells in the Opticell culture system.
An EBV-transformed human B cell line, which produces monoclonal IgM antibodies, was cultured in an immobilized state on a ceramic matrix in the Opticell system. Cell growth, metabolic activity and product formation of these cells were optimized in a single semi-continuous culture of 10 inductions for 29 d. All in all, 19.5 g of IgM were harvested in 193 l of culture supernatant. The average production of IgM in the Opticell unit of 20 l was 0.7 g/d which was obtained even in serum-reduced medium (1%). Under optimal conditions IgM yields were enhanced to 1.5-2.0 g/d. These results indicate that the Opticell is a suitable system for the large scale production of monoclonal antibodies, particularly because the capacity for aeration and pumping of one Opticell unit is sufficient to control 3 growth chambers running in parallel.
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001709:Biotechnology / Q000379:methods; D002448:Cell Adhesion; D002478:Cells, Cultured; D002516:Ceramics; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000502:physiology; D007075:Immunoglobulin M / Q000302:isolation & purification; D010101:Oxygen Consumption
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D005973:Glutamine; D005947:Glucose