title: Unresponsive, a behavioral mutant in Xenopus laevis: electrophysiological studies of the neuromuscular system.
abstract: Normal Xenopus laevis embryos begin movements at 1 day after fertilization. Embryos homozygous for the unresponsive mutation fail to move until 4 days after fertilization (just prior to feeding), after which they recover slowly. Electrophysiological studies were undertaken to determine the focus of this mutation. Formamide treatment of normal embryos was used to produce a phenocopy of the unresponsive condition, permitting direct comparisons between mutant and normal embryos. Intracellular recordings from muscle cells were obtained in formamide-treated and untreated preparations with both normal and unresponsive animals. Local electrical stimulation evoked either isolated endplate potentials and action potentials or after-discharges of these events in all preparations. A decrease in illumination also caused a burst of endplate potentials and action potentials. Therefore, the electrophysiology of the neuromuscular junction in unresponsive appears qualitatively normal; the effect of the mutation on the motor system is probably distal to the neuromuscular junction, either at or subsequent to excitation-contraction coupling.
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D004625:Embryo, Nonmammalian / Q000502:physiology; D005559:Formamides / Q000494:pharmacology; D008027:Light; D008564:Membrane Potentials; D009154:Mutation; D009469:Neuromuscular Junction / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: D005559:Formamides; C031066:formamide