Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction.
The effect of phenylalanine restriction on the level of expression of phenylalanyl-tRNA synthetase from cultured Chinese hamster ovary cells was investigated. By lowering the phenylalanine concentration from 200 to 2 microM, cell growth was arrested, tRNAPhe aminoacylation level was rapidly and specifically decreased and phenylalanyl-tRNA synthetase was derepressed. The progressive 2-fold elevation of phenylalanyl-tRNA synthetase level was determined by activity measurement and immunotitration. None of the other aminoacyl-tRNA synthetases tested were significantly affected.
D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D010053:Ovary; D010649:Phenylalanine / Q000378:metabolism / Q000494:pharmacology*; D010652:Phenylalanine-tRNA Ligase / Q000096:biosynthesis* / Q000235:genetics; D012343:RNA, Transfer / Q000378:metabolism
D010649:Phenylalanine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase