Fasciola hepatica: tegumental surface alterations following treatment in vitro with the deacetylated (amine) metabolite of diamphenethide.
The effect of the deacetylated (amine) metabolite of diamphenethide (10 micrograms/ml) on the tegumental surface of Fasciola hepatica over a 24 h period in vitro has been determined by scanning electron microscopy. Blebbing begins around the oral sucker after 3 h and then passes backwards along the body, reaching the ventral sucker and midbody by 6 h, and finally the posterior end of the body (by 12 h). Initially, the blebs are small, the tegument surrounding the spines is swollen and the tegument generally has a smooth, swollen appearance. This submerges the spines below the body surface. At higher magnification the surface is seen to bear microvillous-like projections in addition to the blebs and surface pitting is deeper than normal. Later on, the blebs increase in size and burst, causing lesions and loss of spines. Lesions begin to appear on the oral cone and ventral sucker after 6 h, in the midbody by 12 h and on the dorsal surface of the posterior region after 24 h. By this time the damage is extensive: around the oral and ventral suckers, and over large areas of the oral cone and midbody region the tegument has been stripped off to expose the basal lamina beneath. The dorsal surface of the fluke is consistently more severely affected than the ventral surface.
D000083:Acetanilides / Q000494:pharmacology*; D000818:Animals; D003957:Diamfenetide / Q000031:analogs & derivatives / Q000494:pharmacology*; D005210:Fasciola hepatica / Q000187:drug effects* / Q000648:ultrastructure; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains
D000083:Acetanilides; C030572:bis(beta-(4-aminophenoxy)ethyl) ether; D003957:Diamfenetide