Alteration of the redox state of NADPH and glutathione in perfused rabbit lung by paraquat.
The glutathione and NADP+ status of perfused rabbit lung was determined both before and after perfusion with 1 mM paraquat. The pulmonary glutathione redox state was similar to that of liver, having a glutathione/glutathione disulfide ratio of about 240. This ratio was lower in lungs perfused with glucose-free medium, a condition in which NADPH probably becomes limiting. Perfusion with paraquat significantly increased the pulmonary glutathione disulfide content, particularly in the absence of glucose, resulting in a glutathione/glutathione disulfide ratio of less than 100. NADP+ levels in rabbit lung were increased approximately two-fold by perfusion with paraquat in medium containing glucose and three-fold in the absence of glucose.
D000818:Animals; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism*; D019803:Glutathione Disulfide; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010269:Paraquat / Q000494:pharmacology*; D010477:Perfusion; D011817:Rabbits
D009249:NADP; D005978:Glutathione; D010269:Paraquat; D019803:Glutathione Disulfide