[Rapid automated synthesis on paper disks of oligodeoxyribonucleotides constituting the promoter fragment of the vaccinia virus genome].
For automation of segmental solid-phase synthesis a simple approach leading to the optimal scheme of synthesis of a large numbers of oligonucleotides in one reaction vessel has been proposed. An advantage of the scheme as compared with synthesis in four reaction vessels is a lower number of condensation steps and increased economy of the process. Sixteen oligodeoxyribonucleotides constituting promoter fragment of the viral genome have been synthesised by the modified segmental method on "Victoriya-2" synthesizer according to the optimal scheme.
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005813:Genes, Synthetic; D005814:Genes, Viral; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D014616:Vaccinia virus / Q000235:genetics*
D009838:Oligodeoxyribonucleotides
Bystryĭ avtomatizirovannyĭ sintez na bumazhnykh diskakh oligodeoksiribonukleotidov, sostavliaiushchikh promotornyĭ fragment genoma virusa ospovaktsiny.