Isolation and partial characterization of 2-microns yeast plasmid as a transcriptionally active minichromosome.
Yeast cell extracts from 2-microns-containing strains (cir+) showed higher transcriptional activity than their corresponding isogenic sets (cir0). These extracts were used to purify transcriptionally active 2-microns minichromosomes in a sucrose gradient. Minichromosomes were transcribed in vitro and, employing hybridization techniques, the RNA synthesized was shown to present 2-microns-specific sequences. This model system should permit the direct study of transcriptionally active eucaryotic chromatin.
D002875:Chromosomes; D004270:DNA, Circular / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D010957:Plasmids; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics*; D014158:Transcription, Genetic
D004270:DNA, Circular; D004271:DNA, Fungal; D012331:RNA, Fungal