title: [Mechanism of action of DNAse from the rat brain].
abstract: The mechanism of action of exodeoxyribonuclease from rat brain predominantly localized in neuronal nuclei was studied. The enzyme which is highly specific towards one-chain DNA can effectively hydrolyze the two-chain DNA of plasmid pBR 322 "activated" by the disruption of the phosphodiester bond in one chain. A kinetic analysis of DNA hydrolysis with a tritium label evenly distributed along the DNA molecule and terminally labeled with phosphate revealed that the enzyme hydrolyzes polynucleotides from the 5'-to the 3'-end via a partially processive mechanism.
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D006868:Hydrolysis; D051381:Rats
chemical_list: D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I
vernacular_title: Mekhanizm deĭstviia DNKazy golovnogo mozga krys.