Method for determination of sodium, potassium, calcium, magnesium, chloride, and phosphate in the rat choroid plexus by flame atomic absorption and visible spectroscopy.
A rapid simple technique for the measurement of Na+, K+, Mg2+, Ca2+, PO4(3-), and Cl- was developed to analyze ion contents in the choroid plexus of the rat. The technique involves digestion in piperidine, precipitation of proteins with HClO4, and analysis of Na+, K+, Ca2+, and Mg2+ by atomic absorption spectroscopy and Cl- and PO4(3-) by visible spectroscopy. The coefficient of variation for the measurement of eight replicates was 1-3% for all ions. Analysis of choroid plexuses from eight rats yielded coefficients of variation of about 6% and the values for Na+, K+, and Cl- compared favorably to previous works. The analytical procedure described in this paper allows the determination of six major physiologic ions in rat choroid plexus (4 mg wet wt).
D000818:Animals; D002118:Calcium / Q000032:analysis; D002712:Chlorides / Q000032:analysis; D002831:Choroid Plexus / Q000032:analysis*; D007477:Ions; D008274:Magnesium / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D011188:Potassium / Q000032:analysis; D051381:Rats; D012015:Reference Standards; D012964:Sodium / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods*; D013054:Spectrophotometry, Atomic
D002712:Chlorides; D007477:Ions; D010710:Phosphates; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium