title: Comparative metabolism of erucic and oleic acid in hepatocytes from rats fed partially hydrogenated marine oil or palm oil.
abstract: Rats were fed for 8 weeks high fat diets containing 20% of either palm oil (PO) or linoleic-supplemented partially hydrogenated marine oil (PHMO). Hepatocytes were isolated from rats fasted overnight. Incubations were performed with albumin bound [10-14C] oleic acid and [14-14C] erucic acid, respectively, for 15 or 60 min. The cell viability was better than 90% after 60 min of incubation. The incorporation of radioactive metabolites into lipids of the cells and of the secreted products was determined. The uptake and channelling of 18:1 and 22:1 into various lipid classes in the hepatocytes were different, but were little influenced by the diets. Erucic acid disappeared more rapidly from the incubation medium than 18:1 at both incubation times and for both dietary groups, and was mainly incorporated into the hepatocyte triglycerides and found in the free fatty acid pool, whereas only small amounts of labelling were detected in the phospholipids. Oleic acid, however, was readily incorporated into both phospholipids and triglycerides, and little was found in the free fatty acid pool. For both the triglycerides and the free fatty acids more than 90% of the radioactivity was represented by the incubation fatty acid. Chain-shortened products were found in the phospholipids, especially after incubation with 22:1. The chain-shortening capacity was somewhat higher in the PHMO group, relative to the PO group. At both incubation times, the major part (80-90%) of the total radioactivity was found in the lipid-soluble fraction of the incubation medium, and for both groups 60-80% still as the original incubation fatty acid. 20-30% of the radioactivity was found in the triglycerides secreted from the cells. Phospholipids were secreted in minor amounts (1-5%). Water soluble products constituted 3-7% at 15 min of incubation, increasing to 10-12% at 60 min, highest when incubating with 22:1 and similar for both dietary groups.
mesh_terms: D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004883:Erucic Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D000073878:Palm Oil; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004042:Dietary Fats, Unsaturated; D004883:Erucic Acids; D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; D010938:Plant Oils; C049811:erucic acid; D019301:Oleic Acid; D000073878:Palm Oil