title: [Study of alkaline hydrolysis of reumycin using NMR spectroscopy].
abstract: The transformation sequence of reumycin in aqueous (D2O) solutions with various pD was studied by NMR spectroscopy and the structures of the yielding products were determined. It was shown that formation of 6-(3-methylureido)-1,2,4-triazine-5-carboxylic acid was the first stage of reumycin alkaline hydrolysis. The subsequent cyclization of this compound resulted in obtaining 5-methyl-5H-imidazo [4,5-e]-1,2,4-triazin-6 (7H) one-4a-carboxylic acid in mono-,di- or trianionic form which depended on the medium pH and was due to dissociation of the carboxylic group, N(4)H group of the triazine ring and N(7)H group of the imidazolidinone ring.
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D012996:Solutions; D014227:Triazines / Q000032:analysis / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D012996:Solutions; D014227:Triazines; C009169:reumycin
vernacular_title: Izuchenie shchelochnogo gidroliza reumitsina metodom spektroskopii IaMR.