Induction of epidermal and hepatic ornithine decarboxylase by a Prudhoe Bay crude oil.
Application of a Prudhoe Bay crude oil (PBCO) to the backs of mice caused a rapid induction of epidermal ornithine decarboxylase (ODC). A maximum induction of over 60-fold was seen at 6 hr after application of 50 microliters of PBCO. Concurrently, epidermal putrescine levels were elevated 4.7-fold over controls. Intraperitoneal administration of PBCO led to a 15-20-fold increase in hepatic ODC activity but to a 45% decrease in the renal enzyme activity. Maximum induction of ODC was seen at 12 hr following the administration of 4 ml/kg body wt of PBCO. Hepatic putrescine levels were elevated 34-fold over controls. Pretreatment of mice with the protein synthesis inhibitor cycloheximide abolished both epidermal and hepatic ODC induction.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D004817:Epidermis / Q000201:enzymology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D010578:Petroleum / Q000633:toxicity*; D012867:Skin / Q000187:drug effects / Q000201:enzymology*
D010578:Petroleum; D003513:Cycloheximide; D009955:Ornithine Decarboxylase