Production of Vi monoclonal antibodies and their application as diagnostic reagents.
Serum antibodies to Vi antigen were detected in mice immunized with the purified antigen but not with Vi-bearing Salmonella typhi whole cells. Fusion of the spleen cells from one of the Vi antibody-producing mice with NSI myeloma cells produced four stable hybridomas that secreted antibodies to Vi. Monoclonal antibodies from these four clones were all of the immunoglobulin G class and, as determined by competition, appeared to have the same epitope specificity. Despite their immunoglobulin G nature, mouse ascitic fluids induced by one of the hybridomas strongly agglutinated the Vi-positive strains of S. typhi, S. dublin, and Citrobacter strain 5396/38. Thus, 10 clinical isolates of S. typhi but not 98 strains of other bacteria were reactive in slide agglutination tests with the monoclonal antibodies.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D002954:Citrobacter / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial; D012475:Salmonella / Q000276:immunology; D012485:Salmonella typhi / Q000276:immunology* / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial