Heterogeneity in high performance liquid chromatography of a variant surface glycoprotein of Trypanosoma brucei.
High performance liquid chromatography (HPLC) procedures have been used to analyze a preparation of the variant surface glycoprotein AnTat 1.1A of Trypanosoma brucei. The native preparation gives several peaks with a high reproducibility both by reverse-phase (RP-) and gel permeation (GP-) HPLC. Under RP-HPLC conditions, nine fractions are fully resolved. The RP-HPLC fractions migrate with the same molecular weight VSG band on polyacrylamide slab gel electrophoresis and no significant differences are observed in amino acid composition among these fractions. The RP-HPLC resolution is found to be related to the ability of the VSG to polymerize as shown using GP-HPLC. These results suggest the existence of a microheterogeneity of the AnTat 1.1A VSG preparation in relation to post-translational modification of the VSG molecule.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006023:Glycoproteins / Q000302:isolation & purification*; D008970:Molecular Weight; D014346:Trypanosoma brucei brucei / Q000032:analysis*; D014643:Variant Surface Glycoproteins, Trypanosoma
D006023:Glycoproteins; D014643:Variant Surface Glycoproteins, Trypanosoma