Quantitative epifluorescence assay of adherence of coagulase-negative staphylococci.
A quantitative epifluorescence assay was developed to assess the adherent qualities of clinical isolates of coagulase-negative staphylococci. This method compared favorably with the standardized tube adherence assay, yet demonstrated a wide range of surface binding affinities among the slime-producing and non-slime-producing strains of coagulase-negative staphylococci examined. This technique can also be adapted to evaluate the adherence of bacteria to other plastics and biomedical materials used to manufacture implantable medical devices.
D001422:Bacterial Adhesion; D003030:Coagulase / Q000378:metabolism; D005470:Fluorometry; D006801:Humans; D008856:Microscopy, Fluorescence; D013210:Staphylococcus / Q000201:enzymology / Q000378:metabolism*
D003030:Coagulase