title: Influence of the Hal locus and standardized stress on antibody response and in vitro reactivity of peripheral blood lymphocytes in pigs.
abstract: The antibody response to two Escherichia coli antigens (O149 and K88) and the in vitro reactivity of peripheral blood lymphocytes (PBLs) to pokeweed mitogen was studied in growing pigs after exposure to varying degrees of stress. Three experimental groups were used; transportation by lorry, transportation by lorry after a previous injection of a tranquillizing drug (amperozide), no transportation but an amperozide injection. Another group was used as a control group. This group was not transported and received no amperozide injection. The animals were the offspring of boars and sows heterozygous (Nn) for the Hal gene, and all 3 Hal genotypes (NN, Nn, nn) were identified and could thus be compared within litters. Immediately after the experimental treatment, the highest PBL reactivity was found for the amperozide-treated animals and for the non-transported animals, with no differences in reactivity between Hal genotypes. Two weeks later, the treatments caused different effects on pigs of the 3 Hal genotypes, both with regard to the PBL reactivity and the IgG response to O149. The NN pigs had a higher PBL reactivity than the nn pigs for all treatments except the 'drug + transport' class where the reverse rank order was found. The NN pigs also had a higher IgG response to O149 than the nn pigs in the 'drug + no transport' class. The amperozide treatment was followed by a higher PBL reactivity in non-transported NN pigs and in transported nn pigs. The amperozide-treated non-transported NN pigs also had a higher IgG response to O149. The highest PBL reactivity and IgG response to O149 were found mainly in pigs with the lowest cortisol levels. Pronounced differences between litters were found for both the antibody response and the PBL reactivity.
mesh_terms: D000758:Anesthesia; D000818:Animals; D005260:Female; D005838:Genotype; D006221:Halothane; D006854:Hydrocortisone / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D010879:Piperazines / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013312:Stress, Physiological / Q000235:genetics / Q000276:immunology*; D013552:Swine / Q000235:genetics / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D010879:Piperazines; D011043:Pokeweed Mitogens; C049058:amperozide; D006221:Halothane; D006854:Hydrocortisone