Effects of acute and chronic ethanol ingestion on pantothenate and CoA status of rats.
Total coenzyme A (CoA) and pantothenate levels in tissues were compared in three studies in which rats were fed Lieber-DeCarli formula diet with either 5% ethanol or maltose-dextrin (controls) for 19-36 d. The heart pantothenate levels of ethanol-fed rats were 51-78% of that in controls. The heart total CoA levels of ethanol-fed rats were 78-90% of that in controls. Liver total CoA and pantothenate levels were lower in ethanol-fed rats than in controls in one and two studies, respectively. Differences were diminished in rats fasted for 24 h. Control and chronic ethanol-treated rats given no ethanol for 24 h were intubated with [1-14C]pantothenate. Accumulation of radioactive pantothenate in hearts of ethanol-treated rats was 58% and accumulation in livers was 75% of that in controls. An acute effect of ethanol on pantothenate conversion to CoA in liver was found in naive rats intubated with 0.5-2.5 g ethanol/kg body weight and given [1-14C]pantothenate by intubation or intraperitoneal injection. In ethanol-treated rats, conversion of [1-14C]pantothenate to CoA was 4-28% of that in controls. These studies demonstrated an effect of chronic ethanol ingestion on pantothenate and total CoA contents of heart and possibly other tissues and an acute effect of ethanol ingestion on [1-14C]pantothenate conversion to CoA in liver.
D000818:Animals; D003065:Coenzyme A / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006321:Heart / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D010205:Pantothenic Acid / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D010205:Pantothenic Acid; D000431:Ethanol; D003065:Coenzyme A