DNA-free liposome-assisted transfection of Streptococcus faecium SF 77R protoplasts.
Two virulent phages of Streptococcus faecium SF 77R were used in DNA-free liposome assisted polyethylene-glycol-mediated protoplast transfection. This procedure, established to develop an effective gene-cloning system, optimized the conditions for DNA uptake by the protoplasts of Str. faecium. Transfection frequencies of up to 10(7)-10(8) P.F.U./microgram of DNA were achieved.
D001435:Bacteriophages / Q000187:drug effects / Q000235:genetics*; D002118:Calcium / Q000494:pharmacology; D004279:DNA, Viral / Q000235:genetics; D008081:Liposomes; D008274:Magnesium / Q000494:pharmacology; D011523:Protoplasts / Q000502:physiology; D013291:Streptococcus / Q000187:drug effects / Q000235:genetics*; D013696:Temperature; D014162:Transfection
D004279:DNA, Viral; D008081:Liposomes; D008274:Magnesium; D002118:Calcium