DNA synthesis in Bloom's syndrome fibroblasts.
The relationship between relative rates of DNA synthesis and DNA content in Bloom's syndrome fibroblasts (BS cells) was investigated by flow cytometry. The cells were pulse labelled with 5-bromo-2'-deoxyuridine (BrdU). The BrdU content and cellular DNA content of individual BS cells were simultaneously measured by flow cytometry in which the cells were double-stained by a FITC-conjugated anti BrdU monoclonal antibody (mAb) for the BrdU content (green) and by PI (propidium iodide) (red) for total DNA content. Their red fluorescence histograms were analysed by a microcomputer to evaluate the cell fractions of each S compartment. The BrdU uptake in the early S phase of BS cells was lower than that of normal cells (fibroblasts from skin of a normal human), whereas the uptake in the middle and late S phase was essentially the same as that of normal cells. The early S phase in BS cells accounted for over 50% of the S phase cells. These findings suggest that, in comparison with normal cells, the rate of DNA synthesis in the early S phase of BS cells is lower, but is identical to controls in the middle and late S phases.
D001816:Bloom Syndrome / Q000378:metabolism* / Q000473:pathology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D007700:Kinetics; D008938:Mitosis
D004247:DNA; D001973:Bromodeoxyuridine