title: [Interaction of gangliosides with low-density proteins from human blood].
abstract: Gangliosides have been shown to modulate the receptor-mediated endocytosis of low density lipoproteins (LDL). The direct interaction of LDL with various gangliosides has been studied. Binding of gangliosides to LDL immobilized on CNBr-Sepharose (LDL-Sepharose) and the influence of gangliosides on the fluorescence of LDL containing anthrylvinyl-labeled sphingomyelin were investigated. The binding of 3H-gangliosides to LDL-Sepharose, as well as fluorescence polarization of LDL were found to depend on the structure and concentration of the gangliosides in a specific saturable manner. The data obtained indicate that gangliosides interact with apolipoprotein B via specific binding sites.
mesh_terms: D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism*; D012685:Sepharose
chemical_list: D005456:Fluorescent Dyes; D005732:Gangliosides; D008077:Lipoproteins, LDL; C021298:ganglioside, GD1a; D005677:G(M1) Ganglioside; D012685:Sepharose
vernacular_title: Vzaimodeĭstvie gangliozidov s lipoproteinami nizkoĭ plotnosti krovi cheloveka.