[Mechanisms of activation of lipid peroxidation and mobilization of the endogenous antioxidant alpha-tocopherol in stress].
Effect of various stress factors (immobilization, catecholamines and glucocorticoids) on lipid peroxidation (LP) and mobilization of alpha-tocopherol in rat blood plasma were studied. Immobilization stress caused an activation of LP in blood plasma, while content of tocopherol was increased in intact animals; in adrenalectomy activation of LP and mobilization of tocopherol were not observed. In intact rats adrenaline and hydrocortisone increased similarly the content of tocopherol in blood plasma, stimulated accumulation of diene conjugates (DC); an increase in the content of Shiff bases was found only after hydrocortisone administration; simultaneous administration of both these drugs led to an increase in DC content as well as the additive effect on tocopherol mobilization was found. In adrenalectomized rats the content of DC and tocopherol were distinctly increased and a decrease in the content of Shiff bases was found after adrenaline administration; hydrocortisone did not affect the content of both DC and Shiff bases, the level of tocopherol was increased only slightly; under conditions of simultaneous administration of these drugs hydrocortisone prevented accumulation of DC in blood plasma, caused by adrenaline; the additive effect on tocopherol mobilization was not observed. The data obtained suggest that glucocorticoids realized their effect on LP via catecholamines and the decrease in the content of Shiff bases, found after adrenaline administration, occurred due to tocopherol mobilization.
D000315:Adrenalectomy; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D012545:Schiff Bases / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013312:Stress, Physiological / Q000378:metabolism*; D014810:Vitamin E / Q000097:blood / Q000378:metabolism*
D008054:Lipid Peroxides; D012545:Schiff Bases; D014810:Vitamin E; D006854:Hydrocortisone; D004837:Epinephrine
Mekhanizmy aktivatsii perekisnogo okisleniia lipidov i mobilizatsiia éndogennogo antioksidanta alpha-tokoferola pri stresse.