Dithiothreitol-induced oxidative damage to thymine and DNA in solution.
Studies on dithiothreitol-induced oxidative damage to thymine and DNA in solution are reported. The major thymine products, cis- and trans-5,6-dihydroxy-5,6-dihydrothymine (thymine glycols), are produced rapidly in 37 degrees C neutral solutions of 10mM thymine and 10mM dithiothreitol. Iron-EDTA enhances while the iron chelator, diethylenetriaminepentaacetic acid, inhibits the reaction. In experiments using 3H-TdR-labeled Escherichia coli DNA, DNA damage was measured as increased ethanol-soluble radioactivity after treatment of the DNA with 5mM dithiothreitol at 45 degrees C. The findings are important with respect to current research interest in thiol radioprotection and thiol-plus-heat toxicity.
D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology*; D005609:Free Radicals; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D012996:Solutions; D013941:Thymine / Q000031:analogs & derivatives / Q000378:metabolism*
D004269:DNA, Bacterial; D005609:Free Radicals; D012996:Solutions; C029389:thymine glycol; D013941:Thymine; D004229:Dithiothreitol