title: Estrogen influences dolichyl phosphate distribution among glycolipid pools in mouse uteri.
abstract: The steroid hormone 17 beta-estradiol dramatically induces uterine N-linked glycoprotein assembly [Dutt, A., Tang, J.-P., Welply, J. K., & Carson, D. D. (1986) Endocrinology (Baltimore) 118, 661-673]. To determine the role that dolichyl phosphate availability plays in this induction, we studied the effects of estrogen priming on the content of dolichyl phosphate and the distribution of dolichyl phosphate among various glycolipids in uteri. Dolichol-linked saccharides were metabolically labeled to equilibrium with either [3H]glucosamine or [3H]mannose and extracted from primary explants of uterine tissue. The amount of dolichol-linked saccharide was calculated from the specific radioactivity determined for the corresponding sugar nucleotides extracted from the tissues. The major dolichol-linked saccharides identified were mannosylphosphoryldolichol (MPD), oligosaccharylpyrophosphoryldolichol (OSL), and N,N'-diacetylchitobiosylpyrophosphoryldolichol (CBL). Estrogen increased the levels of MPD and OSL 4-fold; however, CBL levels did not change. After 3 days of treatment, the levels of these glycolipids were very similar to those in uteri from pregnant mice. Remarkably, MPD constituted 90-95% of dolichol-linked saccharides detected under all conditions. The tissue contents of total dolichyl phosphate and alkali-labile dolichyl phosphate, presumably MPD, were estimated by liquid chromatography. The levels of alkali-labile dolichyl phosphate determined in this way were in good agreement with the values estimated for MPD by metabolic labeling; moreover, alkali-labile dolichyl phosphate constituted 50-98% of the total dolichyl phosphate pool. The variations in MPD content depended upon the steroid hormone influence, most notably that of estrogen.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D004288:Dolichol Phosphates / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005944:Glucosamine / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008358:Mannose / Q000378:metabolism; D051379:Mice; D010052:Ovariectomy; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D014316:Tritium; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004288:Dolichol Phosphates; D006017:Glycolipids; D011106:Polyisoprenyl Phosphates; D014316:Tritium; D004958:Estradiol; D005944:Glucosamine; D008358:Mannose