Pertussis toxin facilitates secretagogue-induced catecholamine release from cultured bovine adrenal chromaffin cells.
Pretreatment of cultured bovine adrenal chromaffin cells with pertussis toxin facilitated nicotine-induced catecholamine release. This facilitation was correlated with the ability of the toxin to catalyze the ADP-ribosylation of an approximately 40-kDa membrane protein. The actions of the toxin were reversed by isonicotinamide, an inhibitor of ADP-ribosylation. Catecholamine release due to high K+ and muscarine was also enhanced by pertussis toxin. In all cases, 45Ca2+ uptake was unaltered in cells treated with the toxin. These results suggest that ADP-ribosylation of a 40-kDa membrane protein facilitates catecholamine release from bovine chromaffin cells without affecting 45Ca2+ uptake.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D007700:Kinetics; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009116:Muscarine / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology*; D037342:Pertussis Toxin; D011188:Potassium / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D002395:Catecholamines; D008565:Membrane Proteins; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D009538:Nicotine; D009116:Muscarine; D037342:Pertussis Toxin; D011188:Potassium; D002118:Calcium