The effect of metyrapone on cytokinin ([8-14C]benzylaminopurine) metabolism in mature green tomato pericarp.
Cytokinin, [8-14C]Benzylaminopurine, metabolism in tomato pericarp was followed during a 3 h period utilizing thin layer chromatography and visualization by fluorography. Fluorography indicated the formation of at least 7 metabolites during 3 h. Cytokinin metabolism was reduced by approximately 40% in 3 h by the presence of 250 microM metyrapone, an inhibitor of cytochrome P-450 related enzyme systems. In the presence of metyrapone, the number of radioactive metabolites on the thin layer plate was reduced from 7 to 4 and 2 of these were unique to the metyrapone-treated sample. These data suggest the initial step in benzylaminopurine metabolism in tomato pericarp may be mediated by a cytochrome P-450 related enzyme system which is altered in the presence of metyrapone.
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D001593:Benzyl Compounds; D003583:Cytokinins / Q000378:metabolism; D007701:Kinetin; D008797:Metyrapone / Q000494:pharmacology*; D010944:Plants / Q000187:drug effects* / Q000378:metabolism; D011687:Purines
D001593:Benzyl Compounds; D003583:Cytokinins; D011687:Purines; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin; D008797:Metyrapone