Chromomycin staining of vertebrate chromosomes: enhancement of banding patterns by NaOH.
A simple technique is described for staining vertebrate chromosomes with the GC-base pair specific DNA fluorochrome, chromomycin A3. Addition of NaOH crystals to a glycerol mounting medium resulted in good differentiation of chromosome bands and eliminated the need for counterstaining.
D000818:Animals; D001482:Base Composition; D002865:Chromomycins; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D002877:Chromosomes, Human / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D006801:Humans; D012972:Sodium Hydroxide; D013045:Species Specificity; D014714:Vertebrates
D002865:Chromomycins; D012972:Sodium Hydroxide; D004247:DNA