Immunochemical characterization of juvenile hormone esterase from different species of Lepidoptera.
Cross-immunoreactivity of juvenile hormone esterase (JHE) from different species was tested using anti-JHE (Trichoplusia ni) (Noctuidae) polyclonal antibody. Partial cross-reactivity was observed between JHE from Hyphantria cunea, Isia isabella (Arctuidae) and Spodoptera exigua (Noctuidae) in immunoblot analysis. Soluble antigen-antibody complex formation was observed between anti-JHE (T. ni) and antigen(s) from Heliothis virescens (Noctuidae) during immunotitration of antigen(s). Using an ELISA method the highest cross-reactivity was observed for both species from Arctuidae and lower cross-reactivity for antigen(s) from H. virescens.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D007915:Lepidoptera / Q000201:enzymology*; D013045:Species Specificity
D000936:Antigen-Antibody Complex; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase