Prostasomes in human semen contain ADP and GDP.
Prostasomes from human semen contained ADP and GDP (mean values 81 +/- 58 respective 71 +/- 46 nmoles/g protein; n = 20) while other nucleoside phosphates were not detected as determined by high performance liquid chromatography. A strong linear relationship (r = 0.80, p less than 0.001) existed between ADP and GDP in prostasomes, while ADP (but not GDP) correlated inversely with semen volume (r = 0.54, p less than 10.02). The possibility is discussed of ADP and GDP being incapsulated within the prostasomes as a macromolecular complex involving stacked nucleotides held together by divalent cations through the phosphate groups.
D000244:Adenosine Diphosphate / Q000032:analysis*; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006150:Guanine Nucleotides / Q000032:analysis*; D006153:Guanosine Diphosphate / Q000032:analysis*; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D009940:Organoids / Q000032:analysis*; D011467:Prostate / Q000166:cytology*; D012661:Semen / Q000032:analysis / Q000166:cytology*
D006150:Guanine Nucleotides; D046911:Macromolecular Substances; D006153:Guanosine Diphosphate; D000244:Adenosine Diphosphate