Cell cycle analysis by flow cytometry in an antibody secreting cell line.
This presentation reports on the routine use of flow cytometric techniques in suspension cultures. As a model system an antibody secreting mouse hybridoma cell line was used to monitor antibody production (a Bromodeoxyuridine specific antibody) and proliferative status as obtained by flow cytometry (FCM) and cell counting.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002453:Cell Cycle; D005434:Flow Cytometry; D006825:Hybridomas / Q000166:cytology*; D051379:Mice
D000911:Antibodies, Monoclonal