Changes in 2-aminoisobutyric acid and cycloleucine uptake produced by 2,4-dichlorophenoxyacetic acid in Chinese hamster ovary cells.
The effect of dichlorophenoxyacetic acid on the transport of two non-metabolizable amino acids, 2-aminoisobutyric acid (AIB) and cycloleucine (CL) was studied in chinese hamster ovary (CHO) cells. The herbicide did not exert any direct effect on the AIB transport. However, when the pesticide was in contact with the cells for 24 h an inhibition of the uptake was observed. Removal of the pesticide from the culture medium restored the influx of the amino acids which reached maximum values 1 h before cell division. The transport kinetics showed changes in Vmax but no variations in Km. These results may indicate that 2,4-dichlorophenoxyacetic acid produces a decrease in the carrier number but without modification of the affinity.
D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity*; D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D003515:Cycloleucine / Q000378:metabolism*; D006918:Hydroxyurea / Q000633:toxicity; D007399:Interphase / Q000187:drug effects; D007700:Kinetics
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D003515:Cycloleucine; C100049:2-aminoisobutyric acid; D015084:2,4-Dichlorophenoxyacetic Acid; D006918:Hydroxyurea