[Attempt to adapt measurement of serum iron levels to the Centrifichem with a direct method using bathophenanthroline sulfonate-guanidine. Value and limits].
The authors have studied a direct automated colorimetric determination of iron serum on Centrifichem. Accuracy and linearity are the same than these obtained by the SFBC recommended method. Precision is poor: the coefficient of variation is about 15% for low values. No alteration in the absorbance is recognized by bilirubin, even in the serum which contains 342 mumol.l-1 of bilirubin. The value of serum iron increases significantly as hemoglobin and copper are added to the serum.
D001322:Autoanalysis / Q000379:methods; D001663:Bilirubin / Q000097:blood; D003124:Colorimetry; D003300:Copper / Q000097:blood; D019791:Guanidine; D006146:Guanidines; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007501:Iron / Q000097:blood*; D010618:Phenanthrolines
D006146:Guanidines; D006454:Hemoglobins; D010618:Phenanthrolines; C017049:bathophenanthroline disulfonic acid; D003300:Copper; D007501:Iron; D019791:Guanidine; D001663:Bilirubin
Essai d'adaptation du dosage du fer sérique sur Centrifichem par une méthode directe à la bathophénanthroline sulfonée-guanidine. Intérêt et limites.