The influence of C18 fatty acids on the growth of a 3T6 derived cell line.
The effects of the 18 carbon chain length series of fatty acids on the viability of a 3T6 derived mouse fibroblast cell line were tested. An overall concentration dependent cytotoxic effect was demonstrated with the saturated fatty acid, and cis- and trans- mono- and dienoic fatty acids; however, within a narrow range of concentrations, cell growth was stimulated. As a result of the increase in cell viability over this concentration range, more than one ID50 value is calculable. The most dramatic cytotoxic effects were shown by cis-C18:3 omega 3 and cis-C18:3 omega 6 with ID50 values of +/- 30 micrograms/ml. cis-C18:4 omega 3 showed an initial cytotoxic effect at fatty acid concentrations of 0-20 micrograms/ml, whilst with fatty acid concentrations of 20-100 mg/ml a cytostatic effect was observed. Furthermore, the effects observed do not appear to be dependent upon the 48 hour incubation period or the presence of fetal bovine serum, and thus would seem to be related to the changes in free fatty acid concentration.
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D000431:Ethanol / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology*; D005312:Fetal Blood; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D051379:Mice; D013997:Time Factors
D003470:Culture Media; D005227:Fatty Acids; D000431:Ethanol