title: Subcellular distribution of glucocorticoid receptor in the neoplastic epithelial duct cell line from human salivary gland.
abstract: Neoplastic epithelial duct cell line from human salivary gland (HSG cell line) contains the specific glucocorticoid receptor. The time course study on the uptake of [3H]triamcinolone acetonide (TA), a synthetic glucocorticoid, by intact HSG cells in a growing monolayer culture showed that translocation of glucocorticoid receptors into nuclei occurred at 37 degrees C, but not at 0 degrees C. To elucidate the subcellular distribution of glucocorticoid receptor from HSG cells, a scaled-up-culture was employed. When the cells were incubated with [3H]TA at 0 degrees C, 94% of the receptors were found in the cytosol fraction, while only 6% of the receptors existed in the nuclei. When the cells were incubated at 37 degrees C, 49% of the receptor complexes were distributed in the nuclei and 74% of these nuclear receptor complexes were extractable with 5 mM pyridoxal phosphate.
mesh_terms: D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D003600:Cytosol / Q000032:analysis; D006801:Humans; D011189:Potassium Chloride / Q000494:pharmacology; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000032:analysis*; D014222:Triamcinolone Acetonide / Q000378:metabolism
chemical_list: D011965:Receptors, Glucocorticoid; D011189:Potassium Chloride; D014222:Triamcinolone Acetonide