Measurement of protein using flow injection analysis with bicinchoninic acid.
We have used the bicinchoninic acid reagent developed by Pierce Chemical Co. to measure proteins in a simple flow injection analyzer. The sensitivity is comparable to that of the Lowry method and no pipetting of reagents is needed. Results are obtained in less than 1 min and samples may be run at a rate of 60/h. The response is linear over a range of protein concentration (0-10 micrograms) and sample size (5-20 microliters) convenient for most analytical requirements. A peristaltic pump, a controlled-temperature water bath, and a spectrophotometer with flow cuvette are the only special apparatus required.
D011506:Proteins / Q000032:analysis*; D011804:Quinolines; D013053:Spectrophotometry / Q000379:methods*
D011506:Proteins; D011804:Quinolines; C047117:bicinchoninic acid