The contribution of corticosterone and testosterone to the suppression of serum LH in hyperprolactinaemic adult male rats with pituitary transplants.
The effects of hyperprolactinaemia on serum levels of LH were investigated in adult male rats of the R X U strain. Hyperprolactinaemia was induced by three pituitary grafts under the kidney capsule, transplanted on day 0 of each experiment. Special attention was paid to the contribution of prolactin-stimulated testes, adrenals and corticosterone. In experiment 1, hyperprolactinaemia significantly reduced the serum concentrations of LH in intact rats. In spite of a significant increase in the serum levels of corticosterone, serum testosterone was not significantly affected by hyperprolactinaemia. The weights of both the adrenals and accessory sex glands were significantly increased at autopsy. In experiment 2, treatment with 10 mg corticosterone s.c. daily from day 14 to day 28 after pituitary grafting significantly reduced serum levels of both LH and testosterone. The suppression of testosterone in the hyperprolactinaemic corticosterone-treated animals was significantly less than in the corticosterone-treated control animals. The weights of the accessory sex glands were significantly increased in the hyperprolactinaemic animals. In experiment 3, rats were adrenalectomized and half of them were substituted with corticosterone. Serum testosterone levels significantly increased in both hyperprolactinaemic adrenalectomized rats and in adrenalectomized corticosterone-treated animals without any significant effect on serum LH. Again the weights of the accessory sex glands were significantly increased in the hyperprolactinaemic animals. In experiment 4, rats were adrenalectomized, gonadectomized and corticosterone treated on day 0 and then implanted with a 2, 1.5 or 1 cm silicone elastomer capsule containing testosterone.(ABSTRACT TRUNCATED AT 250 WORDS)
D000315:Adrenalectomy; D000818:Animals; D003345:Corticosterone / Q000097:blood* / Q000494:pharmacology; D005837:Genitalia, Male / Q000033:anatomy & histology; D006966:Hyperprolactinemia / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010902:Pituitary Gland / Q000637:transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000097:blood*
D013739:Testosterone; D007986:Luteinizing Hormone; D003345:Corticosterone