In vitro distribution of porphyrin metabolites from 10(-3) M delta-aminolevulinic acid in primary neural tissue cultures.
The distribution of porphyrin metabolites (uro-7-6-5-copro-3-proto-heme) from 10(-3) M delta-aminolevulinic acid (ALA) in neuronal and glial primary cultures was examined by an isotopic technique. In both cell types, 8-9% of the total production (cell + medium) was found in the cells and more than 90% in the medium. In both the cells and the medium the predominant porphyrin fraction was uro + 7 carboxylic. The heme fraction of the cells was also very high. Through the exclusion of ALA-synthetase activity, the "Uroporphyria-like-model" of "hepatic porphyria" was demonstrated.
D000622:Aminolevulinic Acid / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002642:Chick Embryo; D002855:Chromatography, Thin Layer; D007982:Levulinic Acids / Q000378:metabolism*; D009457:Neuroglia / Q000032:analysis / Q000378:metabolism*; D009474:Neurons / Q000032:analysis / Q000378:metabolism; D011166:Porphyrins / Q000032:analysis / Q000096:biosynthesis*
D007982:Levulinic Acids; D011166:Porphyrins; D000622:Aminolevulinic Acid