Actin antibodies. Preparation and characterization of antibodies specific for smooth-muscle actin isoforms.
We have determined the specificity of sera elicited by glutaraldehyde-stabilized bovine aortic actin. This modification induces a high titre of antibodies directed against the N-terminal (residues 1-39) and the C-terminal region of smooth-muscle actins. The crude antisera were purified on peptide (corresponding to the 1-9 or 1-8 N-terminal sequences of smooth-muscle isoactins)-polyacrylic-resin columns. By fractionating the antisera we obtained oligoclonal antibody populations specific for each isoactin.
D000199:Actins / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D002417:Cattle; D007536:Isomerism; D009131:Muscle, Smooth, Vascular / Q000276:immunology*
D000199:Actins; D000906:Antibodies