Lipoxin synthesis by arachidonate 5-lipoxygenase purified from porcine leukocytes.
Arachidonate 5-lipoxygenase purified from porcine leukocytes produced several more polar compounds from 5,15-dihydroperoxy-eicosatetraenoic acid added as such or generated from 15-hydroperoxy acid. These polar products with absorption maxima at 301-302 nm and shoulders at 289 nm and 316-317 nm were identified as 5S,6R,15S-11-cis-lipoxin A and its 6-epimer, all-trans-lipoxin A isomers, and all-trans-lipoxin B isomers. Most of these lipoxins were presumably degradation products of a 5,6-epoxy intermediate formed by the catalysis of leukotriene A synthase, an integral part of 5-lipoxygenase. The rate of the enzymatic lipoxin synthesis from 15-hydroperoxy acid was about 6% of arachidonate 5-oxygenation.
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000097:blood* / Q000302:isolation & purification; D001091:Arachidonate Lipoxygenases / Q000097:blood*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis* / Q000302:isolation & purification; D007962:Leukocytes / Q000201:enzymology*; D044045:Lipoxins; D013552:Swine
D006893:Hydroxyeicosatetraenoic Acids; D044045:Lipoxins; C040527:lipoxin A4; D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase