A convenient radiometric assay for flavin-containing monooxygenase activity.
A rapid, convenient assay to determine the activity of the flavin-containing monooxygenase is described. The method is based on direct analysis of quenched incubation mixtures by thin-layer chromatography and utilizes tritiated dimethylaniline as the substrate. The synthesis of the radiolabeled substrate is described. The usefulness of dimethylaniline N-oxide formation as a measure of flavin-containing monooxygenase activity was assessed using the purified hog liver enzyme, hog liver microsomes, and liver microsomes from untreated and phenobarbital-pretreated rats.
D000814:Aniline Compounds / Q000032:analysis; D000818:Animals; D002855:Chromatography, Thin Layer; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D010105:Oxygenases / Q000032:analysis*; D011874:Radiometry / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine
D000814:Aniline Compounds; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming); C005141:dimethylaniline N-oxide