Upregulation of imipramine binding and serotonin uptake by estradiol in female rat brain.
This report describes the effect of chronic estradiol treatment on the serotonin transporter in the female rat brain. Both [3H]imipramine binding and [3H]serotonin uptake increased by 20-30% in the frontal cortex and hypothalamus of ovariectomized rats after 12 days of 17 beta-estradiol treatment. No differences were observed in the binding and uptake parameters as a function of the rats' estrous cycle or in untreated ovariectomized rats, as compared to controls. Estradiol in vitro, inhibits [3H]imipramine binding as well as serotonin uptake in rat brain and human platelets. Like serotonin, estradiol decreases the dissociation rate in vitro of [3H]imipramine from its binding site in a dose-dependent manner.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D001792:Blood Platelets / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D007099:Imipramine / Q000378:metabolism*; D010052:Ovariectomy; D051381:Rats; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D004958:Estradiol; D007099:Imipramine