Inhibition of lipid peroxidation by superoxide dismutase following regional intestinal ischemia and reperfusion.
In a feline model of regional intestinal ischemia, reoxygenation resulted in a rise in the concentration of oxidized glutathione, from 2.3 +/- 0.7 to 4.1 +/- 0.5% of the total glutathione. Also conjugated diene as an indirect measurement for lipid peroxidation increased after reperfusion from 2.5 +/- 0.5 mumol/g to 5.5 +/- 1.2 micrograms mol/g tissue. These results are in line with the hypothesis that ischemia results in an accumulation of hypoxanthine and a conversion of xanthine dehydrogenase into its O2-dependent form. Upon reoxygenation, hypoxanthine can be oxidized giving yield to a burst of O2-. and its interconversion products. These may initiate peroxidative tissue damage. Pretreatment of the cats with superoxide dismutase inhibited the biochemical alterations and protected the tissue from peroxidation damage.
D000818:Animals; D002415:Cats; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007422:Intestines / Q000098:blood supply* / Q000187:drug effects; D007511:Ischemia / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D010477:Perfusion; D013482:Superoxide Dismutase / Q000494:pharmacology*
D008054:Lipid Peroxides; D013482:Superoxide Dismutase