The effects of organochlorine pesticides as inducers of testosterone and benzo[a]pyrene hydroxylases.
p,p'-DDE, phenobarbital, dieldrin heptachlor, chlordane and toxaphene induced rat liver microsomes exhibited increased formation of the 4,5-dihydrodiol, 3,6-quinone, 9- and 3-hydroxymetabolites of benzo[a]pyrene and the latter three compounds also induced an increase in the rate of formation of the 9,10-dihydrodiol metabolite. Lindane was inactive as an inducer of benzo[a]pyrene hydroxylase. With the exception of lindane, all the organochlorine pesticides and PB induced testosterone 16 alpha- and 16 beta-hydroxylases; in contrast lindane induced testosterone 6 alpha-, 7 alpha- and 6 beta-hydroxylases and PB also induced testosterone 15 beta-hydroxylase and androstenedione formation. Using a battery of monooxygenase enzyme assays it was evident that there were significant differences between PB and several organochlorine pesticides as inducers of rat hepatic cytochrome P-450-dependent monooxygenases.
D000633:Aminopyrine N-Demethylase / Q000096:biosynthesis; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001579:Benzopyrene Hydroxylase / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013250:Steroid Hydroxylases / Q000096:biosynthesis*
D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D001564:Benzo(a)pyrene; D006899:Mixed Function Oxygenases; C019647:aldrin epoxidase; D013250:Steroid Hydroxylases; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; C025619:steroid 16-beta-hydroxylase; C022724:testosterone 7-alpha-hydroxylase, hamster; D000633:Aminopyrine N-Demethylase; D010634:Phenobarbital