Comparisons of in vivo and in vitro photosensitivities and DNA repair in fibroblast and keratinocyte cells.
Minimal erythema dose of ultraviolet light correlated significantly with the UV-sensitivity of fibroblast cells from 5 patients with xeroderma pigmentosum, 13 patients with keratinocytic neoplasms of the skin, and 21 control subjects, but not with that of cells from 6 patients with photosensitive dermatitis. In unscheduled DNA synthesis after UV irradiation, the number of grains per nucleus was much less in keratinocytes than in fibroblasts, but the relative dose-response relationship was similar. This indicates that keratinocytes can also be used in vitro UV-sensitivity studies.
D001913:Bowen's Disease / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000528:radiation effects*; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism / Q000528:radiation effects*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D010787:Photosensitivity Disorders / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000378:metabolism
D004247:DNA