Identification of a low molecular weight polypeptide of pregnant bovine uterine origin (LMW-UDF): influence on coagulation system in vitro. Part I.
Acidified extracts of pregnant bovine uterus were found to contain a heparin-affinity polypeptide(s). The eluted peak was assayed in the Chandler loop method for whole blood and fibrin thrombus generation. Significant differences in the mean weight and size of whole blood thrombi (243.5 +/- 50 mg and 3.5 cm +/- 0.6 cm) occurred with 1 microgram of purified polypeptide when compared to control samples containing albumin (47.87 +/- .30 g and 0.8 cm +/- 0.3 cm). This activity was detected by the Chandler loop method with 100 ng to 10 micrograms of protein when analyzed in a dose-response fashion. When tested for fibrin thrombus formation this activity persisted. Experiments with 125I fibrinogen revealed no net increase or decrease of uptake into whole blood thrombi when purified polypeptide was added. The heparin-affinity polypeptide(s) possessed a molecular weight of approximately 6-4 kDa when examined by SDS-PAGE. We have therefore designated this polypeptide as low molecular weight-uterine derived factor (LMW-UDF).
D000818:Animals; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000302:isolation & purification*; D002417:Cattle; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005337:Fibrin / Q000502:physiology; D005340:Fibrinogen / Q000502:physiology; D006493:Heparin; D008970:Molecular Weight; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification / Q000502:physiology*; D011270:Pregnancy, Animal; D014599:Uterus / Q000502:physiology*
D001779:Blood Coagulation Factors; D011257:Pregnancy Proteins; D005337:Fibrin; D005340:Fibrinogen; D006493:Heparin