Characterization, purification, and analysis of solvent yellow 33 and solvent green 3 dyes.
Thin-layer chromatography and high-performance liquid chromatography (HPLC) with linear photodiode array detection (LPDA) were used to separate impurities from two commercial dyes. Gravity flow liquid chromatography was used to purify reference standards of the dyes. Normal phase HPLC with LPDA detection was used to determine purities of submitted samples by comparing responses to those of the reference standards. Electron impact mass spectrometry and nuclear magnetic resonance were utilized to confirm structures of the dyes and their impurities.
D000880:Anthraquinones / Q000032:analysis* / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011804:Quinolines / Q000032:analysis* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
D000880:Anthraquinones; D007202:Indicators and Reagents; D011804:Quinolines; C036239:cyanine green G base; C027012:2-((2'-quinolinyl)-1,3-indandione)