title: Repair of rabbit duodenal mucosa after acid injury in vivo and in vitro.
abstract: Epithelial repair after luminal acid exposure was studied in the rabbit duodenum in vivo and in vitro. Hydrochloric acid (200 mM, 30 min in vivo; 10 mM, 10 min in vitro) caused a uniform damage of the mucosa confined to the villi. After demarcation and detachment of the necrotic tissue the defect was bridged by the remaining viable epithelial cells, resulting in a reduction of villus height. This process of mucosal repair proceeded continuously, so that 9 h after acid damage only 33% of villi in the proximal duodenum and 41% in the distal duodenum remained not fully restored, at both luminal pH 7 and luminal pH 3. The difference is due to the higher acid susceptibility of the distal duodenum. In vitro, most of the mucosal surface was reconstituted 5 h after acid injury, at both luminal pH 7.4 and luminal pH 3. The potential difference declined and reversed after mucosal damage; restitution to original values was only observed at luminal pH 7 in vivo and luminal pH 7.4 in vitro.
mesh_terms: D000818:Animals; D002455:Cell Division; D004386:Duodenum / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D005753:Gastric Mucosa / Q000502:physiology; D006851:Hydrochloric Acid / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009336:Necrosis; D011817:Rabbits; D013997:Time Factors
chemical_list: D006851:Hydrochloric Acid