Preparation of 111In leukocytes after hemolytic removal of erythrocytes.
An optimized procedure is described for isolation and high-efficiency radiolabeling of leukocytes using 111In-oxine. The chief advantages over conventional methods include virtually no loss of leukocytes during washing and separation steps; a significant reduction in the time required to prepare leukocytes for radiolabeling compared to non-hemolytic preparations; a 28% increase in the average labeling efficiency obtained using 111In-oxine; greater than 95% cell viability as measured by the trypan blue exclusion test; elimination of contaminating red blood cells from the leukocyte pellet prior to labeling; and 80% survivability at 15 min post injection (measured as per cent of blood activity on leukocyte fraction).
D002469:Cell Separation; D004912:Erythrocytes; D006461:Hemolysis; D006801:Humans; D007204:Indium; D007553:Isotope Labeling; D007962:Leukocytes; D011868:Radioisotopes
D011868:Radioisotopes; D007204:Indium