title: Polarized attenuated total reflectance spectra of oriented purple membranes.
abstract: The technique of polarized Fourier transform infrared attenuated total reflectance spectroscopy has been applied to the study of oriented purple membranes of Halobacterium cutirubrum. This method offers a fast and simple approach for probing conformations of proteins in-situ and capable of obtaining polarized infrared spectra at an angle of incidence that is much greater than the Brewster angle.
mesh_terms: D001436:Bacteriorhodopsins / Q000302:isolation & purification / Q000378:metabolism*; D006217:Halobacterium / Q000378:metabolism; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods
chemical_list: D001436:Bacteriorhodopsins