A carrier for GABA uptake exists on noradrenaline nerve endings in selective rat brain areas but not on serotonin terminals.
gamma-Aminobutyric acid (GABA) increased in a concentration-dependent way (3-300 microM) the basal release of tritium from rat cerebral cortex and hippocampus synaptosomes, prelabelled with 3H-noradrenaline (3H-NA); however, GABA was ineffective on hypothalamic nerve endings. The effect displayed by low concentrations (less than 10 microM) of GABA was largely bicuculline-sensitive. Muscimol mimicked GABA, while (-)baclofen was inactive. The releasing effects produced by concentrations of GABA higher than 10 microM were largely prevented by SK&F89976A, SK&F100330A and SK&F100561, three novel GABA uptake inhibitors. When present together, GABA uptake blocker and bicuculline counteracted entirely the GABA effects. The basal release of 3H-5-hydroxytryptamine (3H-5-HT) in synaptosomes from various CNS regions was not affected by GABA.


CONCLUSIONS
GABA can enhance 3H-NA release not only through GABA-A receptors but also by penetrating into NA terminals through a GABA uptake system. This implies coexistence of carriers for NA and GABA uptake on a same nerve terminal. The carrier coexistence occurs in selective CNS areas. The phenomenon appears to be transmitter-selective.
D000818:Animals; D001693:Biological Transport, Active; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D009638:Norepinephrine