Further purification of human pituitary-derived chondrocyte growth factor: heparin-binding and cross-reactivity with antiserum to basic FGF.
The previously described human pituitary-derived chondrocyte growth factor (CGF), mitogenic for rabbit fetal chondrocytes, was found to bind to heparin-Sepharose and was eluted with 1.5M NaCl. Further characterization of CGF demonstrated a molecular weight of 18-20 kD and cross-reactivity with antiserum to synthetic bovine basic fibroblast growth factor (FGF1-24). When human pituitaries were homogenized in 0.15 ammonium sulfate (pH 5.5) and the extract chromatographed on heparin-Sepharose, 98% of the mitogenic activity was adsorbed to heparin and eluted with 3M NaCl. These findings indicate that CGF is closely related or identical to basic FGF and that the bulk of mitogenic activity in the human pituitary extracts binds to heparin.
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000302:isolation & purification*; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera; D010902:Pituitary Gland / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D011817:Rabbits
D006133:Growth Substances; D007106:Immune Sera; D011506:Proteins; D005346:Fibroblast Growth Factors; D006493:Heparin