Preparation and characterization of monoclonal antibodies specific for GoCodGdCGoCo duplex with high-anti conformation.
Monoclonal antibodies were prepared using a self-complementary oligonucleotide duplex containing cyclonucleosides with the high-anti conformation as an antigen. A competition ELISA assay showed that the monoclonal antibodies specifically recognized high-anti left-handed oligonucleotides but not oligonucleotides with B- or Z-conformation.
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides