[Interaction of glucocorticoids with receptors of rat liver cells during the growth of Walker carcinosarcoma].
Specific glucocorticoid hormone receptors with Kass = 1.23 X 10(8) M-1 and the content of binding sites 5.59 X 10(-13) moles per mg of protein were found in liver cytosol of intact rats. During growth of Walker carcinosarcoma concentration of binding sites decreased approximately by 40%, whereas the affinity of receptors to the hormone did not mainly altered. At the same time, a considerable decrease was noticed in the content of free binding sites not bound with endogenous corticosteroids. After thermoactivation about 50% of 3H-dexamethasone receptor complexes acquired the ability to bind DNA, while in the same experimental conditions only about 20% of the complexes were activated in cytosol of tumor-bearing rats. With growing of the tumor thermal inactivation of the hormone-receptor complexes was not practically altered both in presence and in absence of molybdate ions. In a solution with low ion strength at 25 degrees a half-life dissociation of 3H-dexamethasone-receptor complexes was equal to 0.042 and 0.084 min-1, respectively, in cytosols of healthy and tumor-bearing rats.
D000818:Animals; D001667:Binding, Competitive; D002279:Carcinoma 256, Walker / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D003907:Dexamethasone
Analiz vzaimodeĭstviia gliukokortikoidov s retseptorami kletok pecheni krys pri roste kartsinosarkomy Uokera.