Possible role of glutathione in cofactor regeneration of dopamine beta-hydroxylase.
The activity of the enzyme dopamine beta-hydroxylase was determined in rat brain stem by a sensitive coupled radiometric assay. The appropriate copper and dilution parameters have been determined for this tissue. Reduced glutathione has been shown to activate the enzyme homogenate at concentrations of 24-240 microM. This paper shows that glutathione cannot contribute to the inhibitory activity coming from the brain stem. A mechanism is proposed for the role of glutathione in cofactor regeneration of dopamine beta-hydroxylase.
D000818:Animals; D001933:Brain Stem / Q000201:enzymology*; D003300:Copper / Q000494:pharmacology; D004299:Dopamine beta-Hydroxylase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D003300:Copper; D004299:Dopamine beta-Hydroxylase; D005978:Glutathione