Reconstitution of the erythrocyte anion transport system: recent progress.
The anion transport system of the human erythrocyte membrane was reconstituted in phosphatidylcholine vesicles by a new procedure. The reconstituted system shows all major properties of the sulfate transport system found in the erythrocyte membrane (Scheuring, U., K. Kollewe, W. Haase, and D. Schubert: J. Membrane Biol. 90, 123-135 (1986)). Modifications of the reconstitution method have now led to increased values of the internal volumes of the protein-lipid vesicles and of the average turnover number of the transport protein (which is now larger than in the erythrocyte membrane). Gel filtration on Sephacryl S-1000 has allowed the isolation of vesicles of a narrow size distribution.
D000838:Anions; D001693:Biological Transport, Active; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D010316:Particle Size; D010713:Phosphatidylcholines; D013431:Sulfates / Q000097:blood
D000838:Anions; D008081:Liposomes; D010713:Phosphatidylcholines; D013431:Sulfates