Radiosensitive antioxidant membrane-bound factors in rat liver microsomes: I. The roles of glutathione and vitamin E.
In vitro lipid peroxidation initiated by NADPH/ADP/Fe3+ reveals an alteration of rat liver microsomal antioxidant factors at day D+4 after whole-body gamma irradiation (8Gy). This alteration is partly reversed by GSH, and more efficiently by Trolox C, a water-soluble analog of vitamin E. This reversion by Trolox C, together with the observed 50% decrease in vitamin E content in microsomes of irradiated rats as compared to those of control animals, indicate that Trolox C acts as a free-radical scavenger like and in place of vitamin E. The antioxidant action of Trolox C is not improved in the presence of GSH, which suggests that the former acts earlier than the latter on the autoxidative free-radical chain reactions. Neither GSH, nor Trolox C, nor both antioxidants totally inhibit in vitro lipid peroxidation, which appeals attention on the possible role of extra-microsomal antioxidant factors, especially cytosolic ones.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology; D005609:Free Radicals; D005978:Glutathione / Q000494:pharmacology*; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D002839:Chromans; D005609:Free Radicals; D008054:Lipid Peroxides; D014810:Vitamin E; D005978:Glutathione; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid