Staphylococcosis of turkeys. 2. Assay of protein A levels of staphylococci isolated from turkeys.
Ninety-eight Staphylococcus aureus isolates and 227 coagulase-negative staphylococcal isolates from turkeys were assayed for protein A content. The amount of protein A of each isolate was quantitated using a direct enzyme-linked immunosorbent assay. Of the S. aureus isolates, 83% possessed some protein A, whereas only 13% of the coagulase-negative staphylococci contained some protein A. No correlation was seen between protein A content and ability to adhere to turkey cells. No differences in virulence were seen between isolates of S. aureus possessing high or low levels of protein A; however, an isolate with no protein A was avirulent.
D000818:Animals; D001422:Bacterial Adhesion; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013205:Staphylococcal Protein A / Q000032:analysis*; D013211:Staphylococcus aureus / Q000032:analysis* / Q000302:isolation & purification / Q000472:pathogenicity; D014422:Turkeys / Q000382:microbiology*; D014774:Virulence
D013205:Staphylococcal Protein A