Fluorescence studies on the Ca2+ and Zn2+ binding properties of the alpha-subunit of bovine brain S-100a protein.
The single cysteine on the alpha-subunit of bovine brain S-100a protein has been modified with the thiol specific probe, Acrylodan. When the labelled apoprotein was excited at 380 nm the fluorescence emission maximum was centered at 484 +/- 2 nm, suggesting that the probe is in a fairly hydrophobic environment. Addition of Ca2+ to the protein caused the emission maximum to undergo a red shift to 504 +/- 2 nm, implying that the fluorophore is now more exposed to the solvent. Zn2+, when added to the protein, induced only a small perturbation and the emission maximum shifted to 481 +/- 2 nm. Ca2+ was able to perturb the fluorophore in the presence of Zn2+. 2-p-Toluidinylnaphthalene-6-sulfonate (TNS)-labelled alpha-subunit when excited at 345 nm exhibited very little fluorescence in the absence of Ca2+. Addition of Ca2+ resulted in an increase in TNS fluorescence accompanied by a blue shift of the emission maximum to 445 +/- 1 nm indicating that the probe in the presence of Ca2+ moves to a hydrophobic domain. The fact that Ca2+ and Zn2+ can perturb the labelled sulfhydryl group in the presence of each other clearly demonstrates that the binding sites for the two metal ions must be different on the alpha-subunit as well as on the S-100a protein.
D015081:2-Naphthylamine / Q000031:analogs & derivatives; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D003545:Cysteine; D009282:Naphthalenesulfonates; D009418:S100 Proteins / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000378:metabolism*
D009282:Naphthalenesulfonates; D009418:S100 Proteins; C009497:2-(4-toluidino)-6-naphthalenesulfonic acid; C038518:acrylodan; D015081:2-Naphthylamine; D015032:Zinc; D003545:Cysteine; D002118:Calcium