Purification of very high density lipoproteins by differential density gradient ultracentrifugation.
Differential density gradient ultracentrifugation procedures, utilizing a vertical rotor, were developed for the preparative purification of very high density lipoproteins (VHDL, density greater than 1.21 g/ml). The VHDLs of several insect species were purified as follows. An initial density gradient ultracentrifugation step removed lipoproteins of lower density from the VHDL-fraction, which partially separated from the nonlipoproteins present in the infranatant. A complete separation was achieved by a second centrifugation step employing a modified gradient system. The use of a vertical rotor and specially designed discontinuous gradients allows a relatively fast, efficient, and economical isolation of the class of very high density lipoproteins. Similar gradient systems should be useful for the detection and purification of VHDLs from other sources.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D007313:Insecta / Q000032:analysis*; D008074:Lipoproteins / Q000302:isolation & purification*; D014819:Vitellogenins / Q000302:isolation & purification
D002352:Carrier Proteins; D008074:Lipoproteins; D014819:Vitellogenins; C030363:lipid transfer protein; C032830:lipophorin