Determination of histamine in biological fluids by capillary isotachophoresis and fluorescence.
Analytical isotachophoresis was used for the determination of histamine in biological fluids. For biological fluids with a very low content of histamine a method was developed that combines the fluorescing condensation product of histamine and o-phthaldialdehyde with the high concentration effect of isotachophoresis. This method permits the determination of histamine in serum and other biological fluids down to less than 3 ng/ml.
D001826:Body Fluids / Q000032:analysis*; D004586:Electrophoresis; D006632:Histamine / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D006632:Histamine