The phosphoenolpyruvate: sugar phosphotransferase system of Streptococcus salivarius. Identification of a IIIman protein.
A double-spontaneous mutant resistant to the growth inhibitory effect of alpha-methylglucoside and 2-deoxyglucose was isolated from Streptococcus salivarius. This mutant strain, called alpha S3L11, did not grow on mannose and grew poorly on 5 mM fructose and 5 mM glucose. Isolated membranes of strain alpha S3L11 were unable to catalyse the phosphoenolpyruvate-dependent phosphorylation of mannose in the presence of purified enzyme I and HPr. Addition of dialysed membrane-free cellular extract of the wild-type strain to the reaction medium restored the activity. The factor that restored the phosphoenolpyruvate-mannose phosphotransferase activity to membranes of strain alpha S3L11 was called IIIman. This factor was partially purified from the wild-type strain by DEAE-cellulose chromatography, DEAE-TSK chromatography, and molecular seiving on a column of Ultrogel AcA 34. This partially purified preparation also enhanced the phosphoenolpyruvate-dependent phosphorylation of glucose, fructose, and 2-deoxyglucose in strain alpha S3L11.
D001426:Bacterial Proteins / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D008757:Methylglucosides; C027020:methylglucoside; D003847:Deoxyglucose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D008358:Mannose