title: Venom resistance in the hedgehog, Erinaceus europaeus: purification and identification of macroglobulin inhibitors as plasma antihemorrhagic factors.
abstract: Hedgehog (Erinaceus europaeus) plasma contains factor(s) which neutralize the hemorrhagic activity of European viper (Vipera berus) venom. These antihemorrhagic factor(s) were purified by gel filtration on Sephacryl S-200 and chromatography on Cibacron Blue Sepharose. The macroglobulin fraction obtained was characterized for proteinase inhibiting activity, molecular weight and purity by polyacrylamide gel electrophoresis and immunological cross-reactivity and was found to contain the three macroglobulin proteinase inhibitors--alpha 2-macroglobulin, alpha 2-beta-macroglobulin and beta-macroglobulin. The purified macroglobulins were totally able to neutralize Vipera berus venom hemorrhagic activity, but no distinction between them in this respect was possible.
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D006364:Hedgehogs / Q000097:blood*; D006470:Hemorrhage / Q000517:prevention & control*; D008259:Macroglobulins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000302:isolation & purification*; D014757:Viper Venoms / Q000037:antagonists & inhibitors*
chemical_list: D001798:Blood Proteins; D008259:Macroglobulins; D011480:Protease Inhibitors; D014757:Viper Venoms; C003941:antihemorrhagic factor