[Modulation of the oxidative metabolism of granulocytes by nonsteroidal anti-inflammatory agents].
In the present study we investigated the influence of the following four nonsteroidal anti-inflammatory drugs on the oxidative response of isolated human granulocytes: carprofen, tenoxicam, indomethacin, piroxicam. The effect of these drugs was assessed by a lucigenin-dependent chemiluminescence. In the experiments carprofen significantly stimulated granulocytes. Indomethacin exhibited a similar effect. However, there was no strong dose-response relation. Preincubation of granulocytes with the four tested drugs resulted only at the highest concentrations in a reduced response of the cells to stimulation with the chemotactic peptide f-met-phe and zymosan-particles. The response of the cells stimulated with zymosan-activated serum as a source of the complement split product C5a and to stimulation with the tumor-promotor phorbol-myristate-acetate was not significantly altered. Only carprofen showed a dose-response-related inhibitory effect. In conclusion, the four tested nonsteroidal anti-inflammatory drugs demonstrate different effects on the oxidative metabolism of human granulocytes. Lucigenin-dependent chemiluminescence, hereby, could be a sensitive tool for measuring the effect of pharmaca on granulocyte function in vitro.
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002227:Carbazoles / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D010101:Oxygen Consumption / Q000187:drug effects*; D010894:Piroxicam / Q000031:analogs & derivatives / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002227:Carbazoles; D010894:Piroxicam; C007005:carprofen; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin; C032801:tenoxicam
Modulation des oxidativen Stoffwechsels von Granulozyten durch nichtsteroidale Antiphlogistika.