Modification of cell surface glycoproteins, macrophage cytostasis, and blood-borne metastatic properties of the murine RAW117 large cell lymphoma by virus superinfection.
Highly metastatic sublines of the murine RAW117-P large cell lymphoma parental line have been selected sequentially in vivo for enhanced blood-borne organ colonization. We found that the subline RAW117-H10, selected 10 times for liver colonization, formed more than 200 times as many liver tumor nodules than the RAW117-P line and showed loss of cell surface RNA tumor virus Mr 70,000 envelope glycoprotein and sensitivity to activated macrophage-mediated cytostasis. Superinfection of RAW117-H10 cells with endogenous RNA tumor virus isolated from RAW117-P cells caused increases in Mr 70,000 envelope glycoprotein expression and sensitivity to activated macrophage-mediated cytostasis concomitant with loss of liver metastatic properties. The results suggest that RAW117 cell surface Mr 70,000 envelope glycoprotein is involved in host macrophage-mediated surveillance mechanisms and metastatic properties.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006023:Glycoproteins / Q000096:biosynthesis*; D008223:Lymphoma / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D008264:Macrophages / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D011070:Poly I-C / Q000494:pharmacology; D012190:Retroviridae / Q000472:pathogenicity*; D014759:Viral Envelope Proteins / Q000096:biosynthesis
D006023:Glycoproteins; D014759:Viral Envelope Proteins; D011070:Poly I-C