The preparation of leukocyte-poor red cells from AS-1 red cells by the spin-cool-filter method.
Ninety-eight units of AS-1 red cells (RBC) were studied to determine the optimal unit age for the preparation of leukocyte-poor red cells by the spin-cool-filter method. After 1 week of storage, the residual leukocyte counts of AS-1 units prepared by this method averaged 0.5 x 10(9) or fewer white cells, which indicated removal of more than 79 percent of the total white cells. The spin-cool-filter method, in conjunction with the rapid degeneration of the white cells in the AS-1 RBC, yielded leukocyte-poor units comparable to those prepared from citrate-phosphate-dextrose-adenine (CPDA-1) RBC.
D000225:Adenine / Q000494:pharmacology; D001793:Blood Preservation / Q000379:methods; D002449:Cell Aggregation / Q000187:drug effects; D002498:Centrifugation / Q000379:methods; D002951:Citrates / Q000494:pharmacology; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007937:Leukapheresis / Q000379:methods*; D007958:Leukocyte Count; D008851:Micropore Filters; D010710:Phosphates / Q000494:pharmacology; D012996:Solutions
C034876:CPDA solutions; D002951:Citrates; D010710:Phosphates; D012996:Solutions; D005947:Glucose; D000225:Adenine