Endotoxin testing in blood.
A chromogenic assay is presented for the determination of endotoxin (LPS) in blood. The assay is based upon the LPS-dependent activation of Limulus amebocyte lysate (LAL), and the subsequent measurement of the activated enzyme with a chromogenic substrate. Handling and stability of the reagents, details of the assay method in tubes or microtiter-plates, recovery of LPS from spiked blood in platelet-rich plasma (PRP), platelet-poor plasma (PPP) and serum, as well as the possibility to store plasma samples will be discussed. A clinical evaluation of the assay is provided by S. van Deventer et al. in this volume.
D002863:Chromogenic Compounds; D004355:Drug Stability; D004731:Endotoxins / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000097:blood; D009930:Organic Chemicals
D002863:Chromogenic Compounds; D004731:Endotoxins; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D009930:Organic Chemicals; C045172:S 2423