Immunohistochemical localization of glucagon-like peptide 1. Use of poly- and monoclonal antibodies.
We report the use of poly- and monoclonal antibodies to study the immunohistochemical distribution of glucagon-like peptide-1 immunoreactivity (GLP-1-IR) in various tissues. The polyclonal antibodies against GLP-1 reacted with pancreatic A cells, enteroglucagon (L) cells in the gut, and some neurons in the central nervous system of all species tested. In pancreas and gut the monoclonal antibodies against GLP-1 exhibited a similar, but species specific distribution, relative to the polyclonal antibodies. The colocalization of GLP-1 and glucagon immunoreactivity in pancreatic, intestinal, and nervous tissues is in agreement with previously reported findings that both peptides are part of a single precursor molecule (preproglucagon).
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D052216:Glucagon-Like Peptide 1; D006651:Histocytochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000032:analysis*; D014018:Tissue Distribution
D000906:Antibodies; D000911:Antibodies, Monoclonal; D010455:Peptides; D052216:Glucagon-Like Peptide 1