Neutrophil mobility during anaesthesia in children. A trial of ascorbate premedication.
In 20 healthy children undergoing elective surgery, mobility of neutrophils, both unstimulated and stimulated by endotoxin, was studied using a millipore filter system with microscopic determination of leading front migration. Paired samples were incubated with 10(-2) mol l-1 calcium ascorbate and ten children also received 10 mg kg-1 ascorbic acid before premedication. Stimulation of mobility was reduced after the opioid premedication (P less than 0.05) in the ascorbate group only, but not significantly during anaesthesia and surgery. A few individuals showed persisting abnormally low values. No effect of ascorbate in vivo or in vitro was demonstrated. There were no infections.
D000293:Adolescent; D000769:Anesthesia, Inhalation; D001205:Ascorbic Acid / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D011229:Preanesthetic Medication; D011897:Random Allocation; D013268:Stimulation, Chemical
D004731:Endotoxins; D001205:Ascorbic Acid