title: In vitro calcification in human osteoblastic cell line derived from periosteum.
abstract: Human osteoblastic cell cultures were established from human periosteum, identified on the basis of high alkaline phosphatase activity in the confluent state. The cells in the growing state showed a fibroblastic morphology and grew to form multiple layers. The cells could spontaneously calcify, and the process was accelerated by alpha-glycerophosphate. Minerals deposited on the cells consisted exclusively of calcium and phosphorus, and matured into hydroxyapatite crystals. The calcification was stimulated by the treatment with 1 alpha,25-dihydroxy-vitamin D3. These results indicate that the osteoblastic cells have the capacity to differentiate into osteocytes and form calcified human bone tissue in vitro.
mesh_terms: D000328:Adult; D002113:Calcification, Physiologic / Q000187:drug effects; D002117:Calcitriol / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010521:Periosteum / Q000166:cytology; D010758:Phosphorus / Q000378:metabolism
chemical_list: D010758:Phosphorus; D002117:Calcitriol; D002118:Calcium