title: Preliminary studies on the primary structure of rat liver dihydropteridine reductase.
abstract: Cyanogen bromide cleavage of reductively alkylated homogeneous rat liver dihydropteridine reductase afforded several peptide fragments identifiable by polyacrylamide electrophoresis of which 6 (CB-1 to CB-6) could be individually isolated by C8 reverse phase HPLC. Each was characterised by N-terminal amino acid analysis and sequence information was derived for CB-1, CB-4 and CB-6. The blocked N-terminal of the holoenzyme was identified as pyroglutamate and the C-terminal sequence was obtained by sequential degradation.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide; D004093:Dihydropteridine Reductase; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009247:NADH, NADPH Oxidoreductases; D010446:Peptide Fragments; D051381:Rats
chemical_list: D010446:Peptide Fragments; D004093:Dihydropteridine Reductase; D009247:NADH, NADPH Oxidoreductases; D003488:Cyanogen Bromide