The quantitation of biotinylated compounds by a solid-phase assay using a 125I-labelled biotin derivative.
The biotin analogue biotinylglycyltyrosine has been synthesized and labelled to a specific activity of 2000 Ci/mmol with 125I. This analogue has been used in conjunction with immobilized streptavidin in an assay which detects as little as 1 fmol biotin or biotinylated molecules in solution. The determination of biotinylated insulin in a tissue extract and the quantitation of a transcription assay are given as examples.
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000032:analysis; D002845:Chromatography / Q000379:methods; D003600:Cytosol / Q000032:analysis; D007457:Iodine Radioisotopes; D008099:Liver / Q000032:analysis; D008832:Microchemistry; D051381:Rats; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014158:Transcription, Genetic
D007457:Iodine Radioisotopes; D012996:Solutions; D001710:Biotin