Phosphorylation of branched-chain 2-oxo acid dehydrogenase complex in isolated hepatocytes.
Hepatocytes, isolated from rats fed a low-protein diet, were incubated with [32P]Pi and the phosphoproteins analysed. Immunoprecipitation using antibody against El of branched-chain 2-oxo acid dehydrogenase complex demonstrated phosphorylation of the alpha-subunit of El. Analysis of the tryptic phosphopeptides from the alpha-subunit indicated that two sites were phosphorylated. 4-methyl 2-oxopentanoate and DL-2-chloro 4-methylpentanoate decreased labelling of both sites. No major direct effects of several hormones on phosphorylation of branched-chain 2-oxo acid dehydrogenase was observed.
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000818:Animals; D002208:Caproates / Q000494:pharmacology; D002478:Cells, Cultured; D006843:Hydrocarbons, Chlorinated; D007651:Keto Acids / Q000494:pharmacology; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism*; D010421:Pentanoic Acids / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D010748:Phosphopeptides / Q000302:isolation & purification; D010750:Phosphoproteins / Q000302:isolation & purification; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains
D002208:Caproates; D006843:Hydrocarbons, Chlorinated; D007651:Keto Acids; D009097:Multienzyme Complexes; D010421:Pentanoic Acids; D010710:Phosphates; D010748:Phosphopeptides; D010750:Phosphoproteins; C016211:alpha-keto-beta-methylvaleric acid; C036533:2-chloroisocaproic acid; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)