title: Improved high-performance liquid chromatographic procedure for the determination of chlormethiazole levels following solid-phase extraction from plasma.
abstract: An improved high-performance liquid chromatographic method for the determination of chlormethiazole levels in plasma is described. The drug is extracted from plasma using commercially available reversed-phase extraction columns; recovery values obtained using Sep-Pak C18 and Bond Elut C1, C2, C4, C6, C8, C18 columns are compared. Separation was achieved by reversed-phase chromatography, using a mobile phase consisting of 0.025 M sodium acetate buffer, pH 4.5-acetonitrile (67:33) at a flow-rate of 1.6 ml/min in conjunction with a 15-cm Jones Chromatography Apex ODS column. The analytical column was protected by a Waters Assoc. Guard-Pak module containing a Guard-Pak CN insert. Using ultraviolet detection at 254 nm chlormethiazole levels in the region of 50 ng/ml can be measured with only 500 microliter of plasma.
mesh_terms: D002719:Chlormethiazole / Q000031:analogs & derivatives / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D007231:Infant, Newborn; D009567:Nitrazepam / Q000097:blood
chemical_list: D007202:Indicators and Reagents; D002719:Chlormethiazole; C034739:clomethiazole edisylate; D009567:Nitrazepam