title: Lipolysis products promote the formation of complexes of very-low-density and low-density lipoproteins.
abstract: In the course of lipolysis, surface lipid products may accumulate on very-low-density lipoproteins (VLDL). To investigate potential lipoprotein interactions mediated by such products, radiolabeled low-density lipoproteins (LDL) were incubated with VLDL and bovine milk lipoprotein lipase in the presence of limited free fatty acid acceptor. With partial VLDL degradation, association of radiolabeled LDL with VLDL remnants or larger aggregates of VLDL density was demonstrated by gradient gel electrophoresis, agarose chromatography, and density gradient ultracentrifugation. VLDL-LDL complex formation was also observed in incubations with lipid extracts from lipolyzed VLDL or with purified palmitic acid in the absence of lipolysis. Complex formation was inhibited by addition of increasing amounts of albumin as free fatty acid acceptor, but could be detected at molar ratios of free fatty acids/albumin that occur in vivo. Composition analysis of LDL reisolated following incubation with VLDL and lipase under conditions favoring partial complex formation revealed enrichment in glycerides and depletion of cholesterol. We conclude that lipolysis products can promote the formation of stable complexes of LDL and VLDL, and that physical interactions of this nature may play a role in the transfer of lipids and apolipoproteins between lipoprotein particles.
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D050556:Fatty Acid-Binding Proteins; D006493:Heparin / Q000494:pharmacology; D050356:Lipid Metabolism; D008066:Lipolysis; D008071:Lipoprotein Lipase / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D009363:Neoplasm Proteins; D012492:Salts / Q000494:pharmacology; D013696:Temperature
chemical_list: D000418:Albumins; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D009363:Neoplasm Proteins; D012492:Salts; D006493:Heparin; D008071:Lipoprotein Lipase