title: [Effect of methotrexate and actinomycin D on the frequency and ratio of early mitotic cells].
abstract: Different methods for obtaining early mitotic chromosomes have been comparatively analyzed. Lymphocyte cultures have been treated with methotrexate, actinomycin-D and with a combination of both agents. It is shown that a high rate of chromosomes in prophase, prometaphase and mid-metaphase can be achieved by the combined methotrexate and actinomycin-D treatment of the lymphocyte culture. Methotrexate treatment of cultures is preferable to obtain the great number of early metaphase chromosomes.
mesh_terms: D002478:Cells, Cultured; D003609:Dactinomycin / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008677:Metaphase / Q000187:drug effects; D008727:Methotrexate / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects*; D011418:Prophase / Q000187:drug effects; D013997:Time Factors
chemical_list: D003609:Dactinomycin; D008727:Methotrexate
vernacular_title: Vliianie metotreksata i aktinomitsina-D na chastotu i sootnoshenie rannemitoticheskikh kletok.