title: The N-CAM D2-protein as marker for synaptic remodelling in the red nucleus.
abstract: We have followed the time-course of changes in the concentration of 3 neuronal and one glial antigen in the red nucleus in rats after unilateral lesion of the cerebellorubral connections. The neuronal markers were the neuronal cell adhesion molecule (N-CAM) D2-protein which is prevalent in newly formed neuronal membranes, and the D1- and D3-proteins, which are found mainly in mature neuronal membranes. The glial marker was S-100, a cytoplasmic protein. Six days after the lesion no changes in the concentration of the markers were found in the partially deafferentiated red nucleus. However, 10 days after the lesion the D2-protein concentration was significantly increased, in contrast to the D1-protein concentration which was decreased. After a further 3 days the D2-protein concentration began to decrease, approaching the still significantly decreased D1-protein concentration. Twenty-one days after the lesion the marker protein concentrations were not significantly changed from normal. However, whereas the concentrations of neuronal membrane markers were lower, the glial S-100 concentration showed a tendency to increase. Furthermore, although the changes in D3-protein concentration were unable to reach statistical significance alone they always followed the direction of D1-protein and were significantly in variance with the changes in D2-protein and S-100 concentrations. Our results support the notion of the N-CAM D2-protein as a useful marker for synaptic turnover in adult brain.
mesh_terms: D000818:Animals; D001927:Brain Diseases / Q000378:metabolism / Q000503:physiopathology; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009473:Neuronal Plasticity; D051381:Rats; D011919:Rats, Inbred Strains; D012012:Red Nucleus / Q000032:analysis* / Q000503:physiopathology
chemical_list: D009419:Nerve Tissue Proteins; C023513:brain-specific protein D2, rat; C028116:nervous system specific protein D1; C028117:nervous tissue specific protein D3