Purification of a bovine liver S6 kinase.
A bovine liver protein serine kinase that catalyzes the multisite phosphorylation of ribosomal protein S6 has been purified to near homogeneity. The enzyme has an Mr of 67,000 on SDS-polyacrylamide gel electrophoresis and an apparent molecular weight of 55,000 on glycerol gradient sedimentation. Its enzymic properties, substrate specificity, molecular size and chromatographic behaviour are similar to those of the principal growth factor--and phorbol 12-myristate 13-acetate-stimulated S6 kinase of cultured cells.
D000818:Animals; D002417:Cattle; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D019893:Ribosomal Protein S6 Kinases; D012269:Ribosomal Proteins / Q000378:metabolism; D013379:Substrate Specificity
D012269:Ribosomal Proteins; D011494:Protein Kinases; D019893:Ribosomal Protein S6 Kinases