A non-competitive enzyme immunoassay of human alpha-lactalbumin in biological fluids and tissue extracts.
A sandwich non-competitive enzyme immunoassay for the measurement of human alpha-lactalbumin in serum, milk, and tumour tissue cytosol was developed using affinity-purified polyclonal antibody adsorbed to solid phase. The detection limits of this procedure in tubes (macromethod) and in wells of microtitre plates (micromethod) are 50 pg and 25 pg/sample, respectively, which means 2.5 micrograms/l of serum at appropriate dilution. The micromethod enables a reduction of the volumes used in the reaction with equal sensitivity, accuracy and reproducibility. A comparison of alpha-lactalbumin concentrations in serum of healthy, pregnant, and lactating women determined by our method with those measured formerly by radioimmunoassays proved the specificity of our procedure.
D000918:Antibody Specificity; D001943:Breast Neoplasms / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007768:Lactalbumin / Q000032:analysis* / Q000097:blood; D007774:Lactation; D008297:Male; D008895:Milk, Human / Q000032:analysis*; D011247:Pregnancy
D007768:Lactalbumin