A circulating complex between ASAT and IgG in serum in an apparently healthy woman.
An elevated level of serum aspartate aminotransferase (ASAT) activity in a subjectively healthy woman was shown to be due to circulating ASAT:IgG complexes. The complexes were demonstrated by taking advantage of the specific interaction between protein A and IgG. Thus, greater than 90% of the patient's ASAT activity in serum could be bound to protein A-Sepharose demonstrating that nearly all the patient's serum ASAT was complexed to IgG. The ASAT-binding capacity of patient serum was calculated as approximately 850 micrograms ASAT/l which corresponds to about 0.01% of patient's IgG.
D000328:Adult; D001219:Aspartate Aminotransferases / Q000097:blood*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D011485:Protein Binding; D012685:Sepharose; D013205:Staphylococcal Protein A
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D012685:Sepharose; D001219:Aspartate Aminotransferases