title: Effect of secretory particles in bovine seminal vesicle secretion on sperm motility and acrosome reaction.
abstract: Particles found in bovine seminal vesicle secretion were enriched by centrifugation. They varied in size and morphology and contained Mg2+,Ca2+-activated ATPase, aminopeptidase A, alanyl aminopeptidase, gamma-glutamyl transpeptidase and dipeptidyl peptidase IV activities. Hyperactivation of sperm motility and the acrosome reaction were induced by these particles in epididymal spermatozoa suspended in a modified Ringer medium. The hyperactivation, analysed with a microscopic slide test, started within minutes of exposure to membrane particles and continued for 3-4 h, during which time spermatozoa underwent the acrosome reaction. Acrosome staining, phase-contrast microscopy and transmission electron microscopy revealed that the acrosome reaction started within 60 min at 37 degrees C and affected up to 80% of spermatozoa in 4 h. These membrane particles differed from those reported previously in other species in enzyme composition, function and organ of origin.
mesh_terms: D000177:Acrosome / Q000502:physiology*; D000818:Animals; D002417:Cattle / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology / Q000502:physiology; D008297:Male; D008561:Membrane Fusion; D012661:Semen / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D012669:Seminal Vesicles / Q000378:metabolism*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*