title: A comparison of two procedures for labelling the surface of the hydatid disease organism, Echinococcus granulosus, with 125I.
abstract: Living, intact protoscoleces of the British horse and sheep strains of Echinococcus granulosus were subjected to surface radioiodination procedures using 125I and Iodogen and 125I-Bolton Hunter reagent. Subsequent combined electron microscopy and autoradiography revealed specific surface membrane labelling with the Iodogen procedure, but significant tegumental labelling with the Bolton-Hunter reagent. The two parasite strains yielded different profiles of electrophoretically separated labelled proteins; the Iodogen method, not surprisingly, resulted in a less complex pattern of labelled polypeptides than the Bolton and Hunter reagent.
mesh_terms: D000818:Animals; D001345:Autoradiography; D004446:Echinococcus / Q000032:analysis* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D006736:Horses; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008854:Microscopy, Electron; D011506:Proteins / Q000032:analysis*; D012756:Sheep; D013388:Succinimides; D014508:Urea / Q000031:analogs & derivatives
chemical_list: D007457:Iodine Radioisotopes; D011506:Proteins; D013388:Succinimides; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; C022581:Bolton-Hunter reagent; D014508:Urea