An improved extraction procedure for an HPLC method for cyclosporin A in whole blood and plasma.
Cyclosporin A (CyA) was assayed by high performance liquid chromatography (HPLC) following extraction of the whole blood or plasma with diethyl ether and chromatography on a Sep-Pak Silica cartridge. Using carefully chosen volumes and ratios of ethyl acetate and hexane in the eluting sequences on the Sep-Pak, the major interferent present in normal blood and serum was eliminated.
D000085:Acetates; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003524:Cyclosporins / Q000097:blood*; D004986:Ether; D006586:Hexanes; D006801:Humans; D012997:Solvents
D000085:Acetates; D003524:Cyclosporins; D006586:Hexanes; D012997:Solvents; D004986:Ether; C007650:ethyl acetate