Identification and quantification of a protein-bound ligand in uremic serum.
A major protein-bound ligand in the serum of chronic hemodialysis patients was isolated from heat-deproteinized uremic serum by "high-performance" liquid chromatography (HPLC). The isolated compound was identified as 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid by use of liquid secondary-ion mass spectrometry and gas chromatography-mass spectrometry. On HPLC, an authentic sample of this compound showed a retention time identical to that of the protein-bound ligand peak. The concentration of the furancarboxylic acid in serum, as estimated by HPLC, is markedly greater in chronic hemodialysis patients than in normal subjects.
D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005663:Furans; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013058:Mass Spectrometry; D011422:Propionates / Q000097:blood*; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood* / Q000628:therapy
D001798:Blood Proteins; D005663:Furans; D011422:Propionates; C041693:3-carboxy-4-methyl-5-propyl-2-furanpropionic acid