One-step purification of mouse monoclonal antibodies by mass ion exchange chromatography on Zetaprep.
A rapid one-step purification procedure was developed to isolate mouse monoclonal antibodies present in the growth medium of hybridoma cultures. The procedure, based on a sulfopropyl mass ion exchange chromatography, yields considerable amounts of purified monoclonal antibody. The immunoglobulins are essentially free of transferrin and albumin, as determined by SDS-polyacrylamide gel electrophoresis.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D003470:Culture Media / Q000032:analysis; D051379:Mice
D000911:Antibodies, Monoclonal; D003470:Culture Media