title: Osteonectin--a differentiation marker of bone cells.
abstract: Bone matrix consists of type-I collagen and non-collagenous proteins. The latter represent only 10% of its total protein content. Since type-I collagen is also present in various other connective tissue sites (e.g., skin) it cannot be considered as bone specific. Among the non-collagenous components osteonectin--a 32 kilodalton (KD) glycoprotein linking mineral to collagen fibrils--is thought to be bone specific due to its biochemical properties. In the present study various skeletal and non-skeletal tissues were investigated for the presence of osteonectin by means of immunocytochemical methods. Two polyclonal antibodies against human and bovine osteonectin were applied. Immunocytochemically, osteonectin could be demonstrated in active osteoblasts and osteoprogenitor cells as well as in young osteocytes, while aged, quiescent osteocytes did not contain the protein, suggesting that the protein is a marker of the osteoblastic functional differentiation of bone cells. Osteonectin was absent in all non-skeletal tissues with the exception of chondrocytes in so-called mineralizing chondroid bone.
mesh_terms: D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D001857:Bone Matrix / Q000166:cytology* / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis*; D002356:Cartilage / Q000473:pathology; D002454:Cell Differentiation; D050723:Fractures, Bone / Q000473:pathology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology; D015676:Osteonectin
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D015676:Osteonectin