Oxidative phosphorylation of creatine by respiring pig heart mitochondria in the absence of added adenine nucleotides.
Isolated pig heart mitochondria were found to form phosphocreatine continuously at the rate of 12.5 +/- 1.8 nmol per min per mg of the mitochondrial protein in the respiration medium without externally added adenine nucleotides, and its formation rate showed a concentration dependency with respect to creatine and phosphate. The synthesis of phosphocreatine was completely inhibited by antimycin, FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), and atractyloside. However, oligomycin had no effect on the rate of phosphocreatine formation. These results are discussed in terms of a model that heart mitochondrial creatine kinase is functionally coupled to the oxidative phosphorylating system via the action of the adenine nucleotide translocase.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D003401:Creatine / Q000378:metabolism*; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010725:Phosphocreatine / Q000378:metabolism*; D013552:Swine
D000227:Adenine Nucleotides; D009840:Oligomycins; D010725:Phosphocreatine; D003401:Creatine