[A method for determining the kinetic characteristics of Ca2+-transporting systems of subcellular structures in smooth muscle].
A simple method is suggested to determine kinetic characteristics of the Ca2+ active transport systems in the smooth muscle. The use of this method has shown that the initial rate of Ca2+ accumulation in the myometrium mitochondria (57.5 nmol per 1 mg of protein/1 min) is 50 times higher than in the sarcolemma vesicles. The calcium capacity of mitochondria (254 nmol per 1 mg of protein) also exceeds essentially (36 times) that of the membrane vesicles. Meanwhile, the Ca2+-transporting systems of these two subcellular structures practically do not differ from each other in the magnitude of the cation semiaccumulation period (4-7 min).
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D007700:Kinetics; D008722:Methods; D009130:Muscle, Smooth / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism*
D002118:Calcium
Metod opredeleniia kineticheskikh kharakteristik Ca2+-transportiruiushchikh sistem subkletochnykh struktur gladkikh myshts.