title: An improved acidified glycerol lysis test (AGLT) used to detect spherocytes in pregnancy.
abstract: The acidified glycerol lysis test (Zanella et al, 1980) had proved unreliable in our hands. We now report the test to be stable and reliable when conducted at 37 degrees C rather than at ambient laboratory temperature as used by earlier workers. With this modification, blood samples were tested from 167 pregnant subjects. Forty-eight were less than 26 weeks pregnant and gave normal AGLT values (T50 greater than 1800 s) while of 119 more than 26 weeks pregnant 57 (48%) gave shortened T50 values indicative of the presence of spherocytes.
mesh_terms: D005260:Female; D005865:Gestational Age; D005990:Glycerol; D006403:Hematologic Tests / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000175:diagnosis*; D013103:Spherocytosis, Hereditary / Q000175:diagnosis*; D013696:Temperature
chemical_list: D005990:Glycerol