Cytochemical study of the fluorescence reaction of chromatin by the porphyrin derivative meso-tetra (4-N-methylpyridyl) porphine.
Application of meso-tetra (4-N-methylpyridyl) porphine (TMPP) on smears of chicken blood revealed an intense red fluorescence on the nuclei under blue-violet exciting light, which seemed to be dependent on the DNA component of chromatin. The possibility that an intercalative binding mechanism based on hydrophobic interaction occurs between TMPP molecules and paired bases of DNA is briefly discussed.
D000818:Animals; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D001952:Bridged-Ring Compounds; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis; D006651:Histocytochemistry / Q000379:methods; D017905:Phosphites; D013050:Spectrometry, Fluorescence
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D001952:Bridged-Ring Compounds; D002843:Chromatin; D017905:Phosphites; C018165:trimethylolpropane phosphite; D004247:DNA