Gas-phase hydrolysis of proteins and peptides.
A simplified gas-phase hydrolysis procedure for proteins and peptides is described. The apparatus consists of a glass vacuum desiccator, a ceramic plate, and a Teflon ring. The method was shown to give reproducible compositions for hydrolysis of human serum albumin and microanalysis of alpha-melanocyte stimulating hormone including the quantitation of as little as one residue of tryptophan. It minimizes sample handling and allows for the simultaneous hydrolysis of a large number of samples.
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005740:Gases; D006801:Humans; D006868:Hydrolysis; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis
D000596:Amino Acids; D005740:Gases; D010455:Peptides; D011506:Proteins; D012709:Serum Albumin; D009074:Melanocyte-Stimulating Hormones