[ATPase inhibitory activities found in the soluble fraction of an ascidian (Halocynthia rotezi) muscle].
Crude extracts obtained by water extraction from the muscle of an ascidian (Halocynthia rotezi) are shown to possess an inhibitory activity on ATPase [(Na, K) ATPase, TF1-ATPase, HMM-ATPase]. Gel filtration of the extract with Sephadex G-10 separated the activity into two peaks, and the latter peak (inhibitor II) was further purified by HPLC. This inhibitor was non-competitive for ATP and associated firmly with the (Na, K) ATPase.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D014020:Tissue Extracts / Q000032:analysis*; D014561:Urochordata; D000097795:ATPase Inhibitory Protein
D011506:Proteins; D014020:Tissue Extracts; D000097795:ATPase Inhibitory Protein