Leishmania braziliensis: protein, carbohydrate, and antigen differences between log phase and stationary phase promastigotes in vitro.
When Leishmania species are grown in vitro, parasites from the stationary phase differ from those in log phase growth in being more infective and more resistant to complement and macrophage mediated killing. In the present study, log phase and stationary phase promastigotes of Leishmania braziliensis panamensis were compared at the molecular level. Differences in polypeptide and glycoprotein composition and antigenicity between log and stationary phase promastigotes of L. b. panamensis were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting; the former showed that two polypeptides were unique to log phase promastigotes and one was unique to stationary phase promastigotes. There were also differences in surface lectin binding characteristics of log and stationary phase promastigotes. Live stationary phase promastigotes bound more concanavalin and lentil lectin than log phase promastigotes, indicating a greater number of mannose residues on their surfaces.
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D037102:Lectins / Q000378:metabolism; D007891:Leishmania / Q000254:growth & development*; D007892:Leishmania braziliensis / Q000032:analysis / Q000254:growth & development* / Q000276:immunology; D011506:Proteins / Q000032:analysis*
D000953:Antigens, Protozoan; D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D011506:Proteins