Microglia in the hypendyma of the rat subcommissural organ following brain lesion with serotonin neurotoxin.
The population of microglial cells in the subependymal layer of the subcommissural organ is sparse in normal adult rats. The number of microglial cells was substantially increased in this area following intraventricular injection of the serotonin neurotoxin 5,6-dihydroxytryptamine (5,6-DHT). In sections of plastic embedded material, 1 micron thick, the majority of phagocytic cells scattered in the subependymal layer had an appearance similar to that described in classical studies of microglial cells. At the electron microscopic level microglial cells exhibited the characteristic elongate nucleus with peripheral chromatin condensation. The perikaryon was scanty, containing strands of rough endoplasmic reticulum. The abundant organelles in the processes included Golgi complexes, mitochondria, rough and smooth endoplasmic reticulum as well as dense and multivesicular bodies. In addition, the processes contained phagocytosed axon terminals originating from the dense serotoninergic input to the subcommissural organ, which had degenerated on accumulating the serotonin neurotoxin. A fraction of the phagocytosed material was contained in subependymal subcommissural organ cells, astrocytes and oligodendrocytes. At the light microscopic level the phagocytosed terminals were visualized histochemically with Schmorl's reaction, which resulted in Prussian Blue precipitates. This allowed screening of microglial cells in complete series of sections through the well-defined subependymal layer of the subcommissural organ.
D015115:5,6-Dihydroxytryptamine / Q000494:pharmacology*; D000818:Animals; D001927:Brain Diseases / Q000139:chemically induced; D005260:Female; D007276:Injections, Intraventricular; D008854:Microscopy, Electron; D009457:Neuroglia / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure; D009490:Neurosecretory Systems / Q000473:pathology*; D010587:Phagocytosis; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000502:physiology*; D013351:Subcommissural Organ / Q000473:pathology*
D012701:Serotonin; D015115:5,6-Dihydroxytryptamine