Quality control of estrogen and progesterone receptor assays.
The intra-laboratory QC materials for estrogen and progesterone receptor assays were prepared using different methods. The most convenient and economical method was found to be the use of lyophilized rat uterine cytosol. The level of receptors remained stable for 12 months when stored at -70 degrees C with the inclusion of 10 mM phenylmethyl sulfonyl-fluoride in the cytosol extraction buffer. For inter-laboratory comparison, we received QC materials from Dr E D Ryan (McMaster University, Canada) monthly and our results fell within +/- 1SD of the mean value of 15 centres from North America and Europe participating in this program.
D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D005260:Female; D006801:Humans; D051379:Mice; D011786:Quality Control; D051381:Rats; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D014599:Uterus / Q000032:analysis
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone