In vivo incorporation of [14C]leucine into brain protein of mice treated with methylmercury and thiol complexes of methylmercury.
The effect of methylmercury and thiol complexes of methylmercury on inhibition of protein synthesis was evaluated. Mice were injected (i.p.) with the following treatments: methylmercuric chloride, methylmercury-glutathione, methylmercury-cysteinylglycine and control (vehicle) for 10 days. Ten animals from each group were injected with [14C]leucine 90 min prior to death. The brains were removed and the extracted protein was subjected to liquid scintillation analysis. Mice receiving the methylmercury and methylmercury-glutathione treatments exhibited significantly greater weight loss than the control while the methylmercury-cysteinylglycine treatment was not significantly different than the control. Incorporation of [14C]leucine into brain protein was significantly depressed in the methylmercury (81% of control) and the methylmercury-glutathione (79% of control) treatments. Protein synthesis in mice receiving the methylmercury-cysteinylglycine complex although not significantly different than the methylmercury treatments was only 92% of the control mice.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004151:Dipeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D005260:Female; D005978:Glutathione / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008767:Methylmercury Compounds / Q000138:chemical synthesis / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*
D004151:Dipeptides; D008767:Methylmercury Compounds; D009419:Nerve Tissue Proteins; C030587:methylmercury cysteinylglycine; C030588:methylmercury glutathione; D005978:Glutathione; D007930:Leucine; C004925:methylmercuric chloride