title: Nuclear Overhauser effects in linear peptides. A low-temperature 500 MHz study of Met-enkephalin.
abstract: Met5-enkephalin was studied in 1 mM solutions in 2H2O at room temperature and in a cryoprotective mixture (DMSOd6/2H2O, mole fraction of DMSO 0.49) in the temperature range 265-298 K. Small positive effects were observed between the ortho and meta protons of Tyr in aqueous solution at room temperature. Intraresidue effects can be made strong and negative by increasing the viscosity of the medium with a combination of cryoprotective mixtures and low temperatures. The use of mixtures with properties very close to water is very promising for conformational studies of enkephalins and of other small linear peptides.
mesh_terms: D003080:Cold Temperature; D004121:Dimethyl Sulfoxide; D004744:Enkephalin, Methionine; D009682:Magnetic Resonance Spectroscopy; D011487:Protein Conformation
chemical_list: D004744:Enkephalin, Methionine; D004121:Dimethyl Sulfoxide