Microdetermination of proteoglycans and glycosaminoglycans in the presence of guanidine hydrochloride.
This report describes a microprocedure that may be used for direct measurement of proteoglycans and glycosaminoglycans, after chromatographic elution with chaotropic reagents. The assay is based on the ability of the sulfated glycosaminoglycans to bind to the cationic dye, dimethylmethylene blue, in solution. Inclusion of guanidinium chloride (0.24 M) in the assay resulted in a stable dye-proteoglycan interaction, but eliminated the interference of other anionic macromolecules such as DNA. The assay is rapid, sensitive, and reproducible and therefore useful for processing several samples. Finally, the procedure can be used for quantitative determination of several types of proteoglycans and glycosaminoglycans.
D000818:Animals; D004247:DNA; D006025:Glycosaminoglycans / Q000032:analysis*; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D008751:Methylene Blue / Q000031:analogs & derivatives; D008832:Microchemistry / Q000379:methods; D011509:Proteoglycans / Q000032:analysis*
D006025:Glycosaminoglycans; D006146:Guanidines; D011509:Proteoglycans; D004247:DNA; D019791:Guanidine; D008751:Methylene Blue; C016401:1,9-dimethylmethylene blue