The formulation of buffers and media for enzyme histochemistry.
Buffer solutions and incubating media for enzyme histochemistry are discussed in terms of pH, ionic strength and buffering capacity. A specially written program is presented. This program enables (i) buffers and media of known pH and ionic strength to be formulated; (ii) the ionic strength of a buffer solution of known molarity, and (iii) the thermodynamic acid dissociation constant of a buffer substance, to be calculated.
D002021:Buffers; D004798:Enzymes / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D012984:Software; D012996:Solutions; D013816:Thermodynamics
D002021:Buffers; D004798:Enzymes; D012996:Solutions