title: Metabolism of three trichothecene mycotoxins, T-2 toxin, diacetoxyscirpenol and deoxynivalenol, by bovine rumen microorganisms.
abstract: The three trichothecene mycotoxins T-2 toxin, diacetoxyscirpenol (DAS) and deoxynivalenol (DON) were incubated in vitro for 12, 24 and 48 h with rumen microorganisms obtained from a fistulated dairy cow. Gas chromatographic and gas chromatographic-mass spectrometric analyses of extracts indicated all three toxins were biotransformed to a variety of deepoxy and deacylated products. DON was partially converted to a product identified as deepoxy DON. DAS was rapidly converted to four products including 15-monoacetoxyscirpenol (MAS), scirpentriol and two new compounds identified as 15-acetoxy-3 alpha,4 beta-dihydroxytrichothec-9,12-diene (deepoxy MAS) and 3 alpha,4 beta,15-trihydroxytrichothec-9,12-diene (deepoxy scirpentriol). T-2 toxin was also completely biotransformed to the products HT-2, T-2 triol and two new metabolites identified as 15-acetoxy-3 alpha,4 beta-dihydroxy-8 alpha-(3-methylbutyryloxy) trichothec-9,12-diene (deepoxy HT-2) and 3 alpha,4 beta,15-trihydroxy-8 alpha-(3-methylbutyryloxy)trichothec-9,12-diene (deepoxy T-2 triol).
mesh_terms: D000818:Animals; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001711:Biotransformation; D002417:Cattle; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D012417:Rumen / Q000382:microbiology*; D012717:Sesquiterpenes / Q000378:metabolism*; D013605:T-2 Toxin / Q000378:metabolism*; D014255:Trichothecenes / Q000378:metabolism*
chemical_list: D012717:Sesquiterpenes; D014255:Trichothecenes; D013605:T-2 Toxin; C007262:deoxynivalenol; C001809:diacetoxyscirpenol