Preparation of standards and determination of sizes of long-chain polyphosphates by gel electrophoresis.
Procedures are presented for isolating fractions of long-chain polyphosphates which have a narrow range of sizes and for determining their chain lengths. The polyphosphates are isolated by elution from preparative polyacrylamide gels. Then, the lengths of these polymers are determined by a method of successive approximations of length from data obtained by electrophoresis on several different gels of varying polyacrylamide concentrations. Once sized, these isolated polyphosphates may be used as electrophoresis standards, making it possible to rapidly and accurately ascertain the size of other samples having unknown chain lengths. By comparison with two other procedures for sizing polyphosphates, it is shown that the method is definitely valid to a length of 450 and most likely to a length of at least 900. This electrophoresis procedure allows, for the first time, the determination of the range of sizes present and the average chain length with only 2-20 micrograms of polyphosphate.
D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005941:Glucokinase / Q000032:analysis; D011122:Polyphosphates / Q000032:analysis*; D012015:Reference Standards
D011122:Polyphosphates; D004247:DNA; D005941:Glucokinase