[Specificity of action of DNA methylases BcnI, CfrI and Cfr10I].
The site specificity of three DNA methylases BcnI, CfrI and Cfr10I was determined to be 5'Cm4C(C/G)GG, 5'PyGGm5CCPu and 5'Pum5CCGGPy, respectively. Using the modification methylases under investigation with known restriction endonucleases, fourteen new DNA cleavage specificities can be created. Some aspects of the use of restriction endonucleases in DNA methylation analysis are discussed.
D001407:Bacillus / Q000201:enzymology; D001483:Base Sequence; D002954:Citrobacter / Q000201:enzymology; D004247:DNA / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D015257:DNA-Cytosine Methylases; D008745:Methylation; D008780:Methyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
D004247:DNA; C039051:DNA modification methylase BcnI; C052231:DNA modification methylase Cfr10I; C052232:DNA modification methylase CfrI; D015257:DNA-Cytosine Methylases; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases
Spetsifichnost' deĭstviia DNK-metilaz BcnI, CfrI i Cfr10I.