title: The effects of various nutritional supplements on the growth, migration and differentiation of Xenopus laevis neural crest cells in vitro.
abstract: This study investigates the nutritional requirements of Xenopus laevis neural crest cells and melanophores developing in vitro. A comparison is made between the growth and differentiation of cells in serum-containing medium and a chemically defined, serum-free medium that we have designed. Our chemically defined medium is more efficient than serum-supplemented medium in promoting proliferation of these cells. Several supplements are required to enhance culture development. These include insulin, alpha-melanocyte stimulating hormone, somatotropin, luteotrophic hormone, linoleic acid, uridine, and putrescine. In addition, collagen and fibronectin provide the most conducive environment tested for cell migration and adhesion.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D003470:Culture Media; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D003470:Culture Media