Coal fly ash induces hepatic and pulmonary cytochrome P-450 and sigma-aminolevulinic acid synthetase in rats.
The effect of intratracheal administration of coal fly ash, its benzene-soluble and benzene-insoluble fractions has been studied on the levels of hepatic and pulmonary cytochrome P-450, cytochrome b5, and the activities of sigma-aminolevulinic acid synthetase and heme oxygenase. Fly ash and both its fractions significantly increased the levels of hepatic and pulmonary cytochrome P-450. Benzene-soluble and benzene-insoluble fractions of coal fly ash significantly increased the levels of cytochrome b5 also in both lung and liver. Fly ash and both its fractions increased the activity of sigma-aminolevulinic acid synthetase and reduced the activity of heme oxygenase in lung and liver. Glass bead particles of similar size did not show any effect on hepatic and pulmonary cytochrome P-450 and cytochrome b5.
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis*; D000818:Animals; D002244:Carbon / Q000494:pharmacology*; D003031:Coal; D060729:Coal Ash; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D003573:Cytochrome b Group / Q000096:biosynthesis; D015786:Cytochromes b5; D004790:Enzyme Induction; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis; D008168:Lung / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D052638:Particulate Matter; D051381:Rats; D011919:Rats, Inbred Strains
D003031:Coal; D060729:Coal Ash; D003573:Cytochrome b Group; D052638:Particulate Matter; D002244:Carbon; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D006419:Heme Oxygenase (Decyclizing); D000624:5-Aminolevulinate Synthetase