In vitro effects of organic solvents on immunity indicators in serum.
Serum treatment in vitro with organic solvents (chloroform, ether, toluene) failed to produce an effect on immunoglobulin levels and activity. After chloroform and ether treatment, no complement activity could be determined, with chloroform-treated serum beginning to express anticomplement activity against autologous, allogenic and xenogenic sera. The classical pathway of complement activation (C1, C4, C2, C3) was primarily inhibited, whereas the alternative pathway remained unaffected. Chloroform-treated sera exhibited significantly declined levels of C1-INH, C3 and C4 as well as of circulating immune complexes. Toluene did not influence any of the parameters tested, while ether blocked complement activity without affecting either the concentration or activity of the other components under investigation. The obtained findings are discussed from the aspect of organic solvent applications in preparing immune products and determining immunity indicators in the serum or other biological fluids.
D000906:Antibodies / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis*; D002725:Chloroform / Q000494:pharmacology*; D003167:Complement Activation / Q000187:drug effects*; D003174:Complement C1 Inactivator Proteins / Q000378:metabolism; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D003170:Complement Pathway, Alternative / Q000187:drug effects; D003171:Complement Pathway, Classical / Q000187:drug effects; D003165:Complement System Proteins / Q000378:metabolism; D004986:Ether / Q000494:pharmacology*; D005019:Ethyl Ethers / Q000494:pharmacology*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D014050:Toluene / Q000494:pharmacology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003174:Complement C1 Inactivator Proteins; D003176:Complement C3; D003181:Complement C4; D005019:Ethyl Ethers; D007136:Immunoglobulins; D004986:Ether; D014050:Toluene; D002725:Chloroform; D003165:Complement System Proteins