title: Alterations in carotenoid metabolism during ochratoxicosis in young broiler chickens.
abstract: The mechanism by which ochratoxin impairs the ability of chickens to utilize dietary carotenoids for carcass pigmentation was investigated. Graded doses of pure ochratoxin A (0, .5, 1.0, 2.0, and 4.0 micrograms of toxin/g of feed) were incorporated into a white corn-soy diet supplemented with an efficiently used oxycarotenoid (110 micrograms free lutein/g) and fed to broiler chicks from day of hatch to 3 weeks of age. Concentrations of free lutein and its metabolites, lutein diester, lutein monoester, and oxolutein, in the jejunal contents, jejunal mucosa, serum, liver, and toe web from these birds were measured by high performance liquid chromatography. Based on the threshold level of ochratoxin required for an effect on the concentrations of carotenoids and on the severity of the effect, five separate loci for the action of ochratoxin on carotenoid metabolism were detected: dilution of carotenoids in intestinal contents, depressed uptake by intestinal mucosa, depressed transport in serum, altered accumulation in liver, and altered acylation steps in the integument.
mesh_terms: D000818:Animals; D002338:Carotenoids / Q000097:blood / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005766:Gastrointestinal Contents / Q000032:analysis; D007583:Jejunum / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000032:analysis / Q000378:metabolism; D008297:Male; D009793:Ochratoxins / Q000506:poisoning*
chemical_list: D009793:Ochratoxins; D002338:Carotenoids