title: Release of core DNA from nucleosomal core particles following (ADP-ribose)n-modification in vitro.
abstract: Isolated nucleosomal core particles from rat liver chromatin were ADP-ribosylated in vitro and the consequences of this modification on intranucleosomal DNA-protein interactions were studied by retention gel analysis. A separating force of 11 fN caused the complete release of 146 bp DNA fragments from ADP-ribosylated core particles, while the DNA of unmodified core particles remained protein-associated. We conclude that posttranslational (ADP-ribose)n-modification of chromatin proteins may reduce DNA-protein interactions at the nucleosomal level of chromatin organization.
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D009707:Nucleosomes / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D000246:Adenosine Diphosphate Ribose; D004247:DNA