Synthesis of methyl-branched fatty acids from methylmalonyl-CoA by fatty acid synthase from both the liver and the harderian gland of the guinea pig.
Partially purified fatty acid synthase preparations from both the liver and the harderian gland of guinea pig showed the same relative rates of utilization of methylmalonyl-CoA when compared to malonyl-CoA. Radio gas-liquid chromatographic analysis of the products generated from [methyl-14C]methylmalonyl-CoA and from [2-14C]malonyl-CoA in the presence of unlabeled methylmalonyl-CoA showed that the enzyme from both tissues generated identical mixtures of branched fatty acids. Therefore, it is concluded that the production of methyl-branched acids only by the harderian gland is not due to any unique specificity of the fatty acid synthase of this gland, in contrast to the conclusion reached from results obtained from mass spectrometry of the products generated by crude extracts (Y. Seyama, H. Otsuka, A. Kawaguchi, and T. Yamakawa J. Biochem. 90, 789-797, 1981).
D000214:Acyl Coenzyme A / Q000031:analogs & derivatives*; D000818:Animals; D002850:Chromatography, Gel; D064429:Fatty Acid Synthases / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis*; D006168:Guinea Pigs; D006243:Harderian Gland / Q000201:enzymology*; D007765:Lacrimal Apparatus / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008316:Malonyl Coenzyme A / Q000031:analogs & derivatives* / Q000378:metabolism; D008745:Methylation
D000214:Acyl Coenzyme A; D005227:Fatty Acids; C015357:methylmalonyl-coenzyme A; D008316:Malonyl Coenzyme A; D064429:Fatty Acid Synthases