title: Experimental stress ulcer and gastric catecholamine contents in spontaneously hypertensive rats.
abstract: To determine the role of the sympathetic nervous system in the development of stress ulcer, we carried out experiments on spontaneously hypertensive rats (SHR) exposed to restraint and water immersion stress for 7 h. Normotensive Wistar-Kyoto rats (WKY) were used as controls. Catecholamine (CA) contents in gastric tissues, divided into mucosal and muscular layers of both antrum and corpus, were quantitated by high-performance liquid chromatographic electrochemical detection (HPLC-EC). The stress ulcer formation was much less frequently induced in SHR than in WKY. The noradrenaline (NA) contents in all regions of gastric tissues were higher in SHR than in WKY. The contents of adrenaline (A) and dopamine (DA), present in small quantities in gastric tissues showed no difference between SHR and WKY. After exposure to stress, the NA contents in mucosal and muscular layers of the gastric corpus decreased significantly in both SHR and WKY, whereas the value remained higher in the former. On the contrary, a remarkable increase of A contents (probably released from the adrenal medulla by the stress) was observed in all gastric tissues, of both SHR and WKY. Increase of the A contents in the mucosal layer was remarkable in the SHR. The DA contents increased in both strains. These results suggest that the peripheral sympathetic hyperfunction in the stomach in the SHR may have an inhibitory role in stress ulcer formation.
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D011706:Pyloric Antrum / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013276:Stomach Ulcer / Q000209:etiology / Q000378:metabolism*; D013312:Stress, Physiological / Q000150:complications*; D013564:Sympathetic Nervous System / Q000503:physiopathology
chemical_list: D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine