An attempt to determine lipid peroxides with polyethylene glycol-modified hemin.
Hemin, having two carboxyl groups, was coupled with alpha-(3-aminopropyl)-omega-methoxypoly(oxyethylene) through the acid-amide bond formed with carbodiimide. The modified hemin catalyzed the peroxidase reaction in 1,1,1-trichloroethane using benzoyl peroxide or peroxides in unsaturated fatty acids as the hydrogen acceptor and leuco crystal violet as the hydrogen donor. A basic study on quantitative microanalysis of the lipid peroxides was attempted.
D006418:Heme / Q000031:analogs & derivatives; D006427:Hemin; D008054:Lipid Peroxides / Q000032:analysis*; D011092:Polyethylene Glycols; D013055:Spectrophotometry, Infrared
D008054:Lipid Peroxides; D011092:Polyethylene Glycols; D006418:Heme; D006427:Hemin