title: Upregulation of imipramine binding and serotonin uptake by estradiol in female rat brain.
abstract: This report describes the effect of chronic estradiol treatment on the serotonin transporter in the female rat brain. Both [3H]imipramine binding and [3H]serotonin uptake increased by 20-30% in the frontal cortex and hypothalamus of ovariectomized rats after 12 days of 17 beta-estradiol treatment. No differences were observed in the binding and uptake parameters as a function of the rats' estrous cycle or in untreated ovariectomized rats, as compared to controls. Estradiol in vitro, inhibits [3H]imipramine binding as well as serotonin uptake in rat brain and human platelets. Like serotonin, estradiol decreases the dissociation rate in vitro of [3H]imipramine from its binding site in a dose-dependent manner.
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D001792:Blood Platelets / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D007099:Imipramine / Q000378:metabolism*; D010052:Ovariectomy; D051381:Rats; D012701:Serotonin / Q000378:metabolism*
chemical_list: D012701:Serotonin; D004958:Estradiol; D007099:Imipramine