Longitudinal flow of macromolecules in the endolymphatic space of the rat. An autoradiographical study.
Secretion and resorption of sulphated glycoproteins by the epithelial lining of the endolymphatic space of the rat were studied autoradiographically with the use of 35SO4 as a selective marker. This isotope was found to be incorporated into the sensory area of cristae and maculae and a small area of epithelial cells adjacent to the sensory epithelium. Thereafter 35S-labelled glycoproteins were secreted into cupulae and otolithic membranes. No such process could be established in the tectorial membrane. From the gradual disappearance of 35S-labelled glycoproteins from cupulae and otolithic membrane coupled with an accumulation of this substance in the lumen of the endolymphatic sac, the existence of a longitudinal flow under physiological conditions could be concluded. Resorption of glycoproteins by the saccus epithelium was found to be very slow. Accumulation of 35S-labelled glycoproteins in the endolymphatic duct after saccus obstruction indicates that, in addition to the endolymphatic sac, also the duct contributes to the mechanism underlying the longitudinal flow of macromolecules.
D000818:Animals; D001345:Autoradiography; D004710:Endolymph / Q000502:physiology*; D004711:Endolymphatic Duct / Q000502:physiology; D004712:Endolymphatic Sac / Q000502:physiology; D006023:Glycoproteins / Q000378:metabolism; D007761:Labyrinthine Fluids / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D006023:Glycoproteins