Characterization of protamines from four avian species.
No data are available on the protamines of birds, with the exception of galline. We have characterized the protamines from four species of birds belonging to four different orders. All of them have very similar properties. They have been purified by carboxymethylcellulose chromatography and analyzed with respect to amino acid composition and electrophoretic behaviour. They are very arginine-rich proteins (63.4-67.3%) but do not contain lysine. Serine (12.0-18.2%), tyrosine (5.8-9.0%) and glycine (4.5-7.1%), along with arginine, make up the bulk of the amino acid residues in these molecules. The electrophoretic mobility of bird protamines in acetic acid-urea-polyacrylamide gels is intermediate between that of somatic histones and salmine. The molecular size, estimated from amino acid analysis and electrophoretic migration, is 65 +/- 5 amino acid residues.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001717:Birds / Q000378:metabolism*; D010856:Columbidae / Q000378:metabolism; D003370:Coturnix / Q000378:metabolism; D004372:Ducks / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D010240:Parakeets / Q000378:metabolism; D011479:Protamines / Q000032:analysis* / Q000302:isolation & purification; D013045:Species Specificity; D013087:Spermatids / Q000032:analysis; D013094:Spermatozoa / Q000032:analysis
D000596:Amino Acids; D011479:Protamines