title: Dependence of intestinal amino acid uptake on dietary protein or amino acid levels.
abstract: To understand how intestinal amino acid (AA) transport is regulated by dietary substrate levels, we measured uptake of seven AAs and glucose across the jejunal brush-border membrane of mice kept on one of three isocaloric rations differing in nitrogen content. In the high-protein ration, uptake increased by 77-81% for the nonessential, less toxic AAs, proline, and aspartate but only by 32-61% for the more toxic essential AAs tested. In the nitrogen-deficient ration, uptake decreased for the nonessential aspartate and proline but stayed constant or increased for essential AAs and for the nonessential alanine. These patterns imply independent regulation of the intestine's various AA transporters. With decreasing dietary AA (or protein), the imino acid and acidic AA "private" transporters are repressed, while activities of the basic AA transporter and the neutral AA "public" transporter decrease to an asymptote or else go through a minimum. These regulatory patterns can be understood as a compromise among conflicting constraints imposed by protein's multiple roles as a source of calories, nitrogen, and essential AAs and by the toxicity of essential AAs at high concentrations.
mesh_terms: D000596:Amino Acids / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D004032:Diet; D004044:Dietary Proteins / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009584:Nitrogen / Q000172:deficiency; D010539:Permeability; D014018:Tissue Distribution
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D005947:Glucose; D009584:Nitrogen