Lactobacillus protoplast transformation.
A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.
D004269:DNA, Bacterial / Q000235:genetics; D007778:Lactobacillus / Q000235:genetics*; D010957:Plasmids; D011092:Polyethylene Glycols; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D011092:Polyethylene Glycols