Tubulin oligomers and microtubule assembly studied by time-resolved X-ray scattering: separation of prenucleation and nucleation events.
This paper describes a time-resolved X-ray scattering study of microtubule assembly by synchrotron radiation. The method is complementary to light scattering but allows a better distinction between oligomeric and polymeric assembly states. With an improved rapid temperature jump device, it is shown that temperature-induced microtubule assembly is preceded by prenucleation and nucleation events involving oligomers of tubulin, in analogy with earlier results from near-equilibrium temperature scans. In general, the two phases closely overlap, but in certain conditions they can be observed separately. The prenucleation events seen by X-rays can be described as a rapid temperature-dependent equilibrium, with ring oligomers dissociating into smaller oligomers and subunits at elevated temperature. Different solution conditions affect mainly the time lag between the prenucleation and nucleation phases; this in turn determines the apparent magnitude of the prenucleation steps. By contrast, the temperature dependence of the equilibrium between the prenucleation oligomers shows little influence on solution conditions. The results suggest that the ring-forming and tubule-forming assembly modes of tubulin are governed by different interactions between subunits, although they may be based on a pool of similar intermediates.
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D008954:Models, Biological; D011487:Protein Conformation; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism*; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D014404:Tubulin