[Inhibition of influenza virus A RNA-polymerase activity by various 3'-amino-3'-deoxy- and 3'-azido-3'-deoxyribonucleoside-5'-triphosphates].
The effects of 3'-amino-3'-deoxy- and 3'-azido-3'-deoxyribonucleoside-5'-triphosphates on the RNA synthesis catalyzed by influenza virus A RNA polymerase were studied. All nucleotide analogues tested decreased the RNA synthesis twofold at the inhibitor: substrate ratio about 1:5 (in moles). The hypothetic mechanism of inhibitors action based on the incorporation of inhibitors into the 3'-termini of the RNA chains and subsequent blocking of the RNA chains elongation is proposed. The nucleotide analogues under investigation were several times more effective as compared with the ribavirine 5'-triphosphate, a well-known inhibitor of influenza A virus reproduction.
D001386:Azides / Q000494:pharmacology; D003854:Deoxyribonucleotides / Q000494:pharmacology*; D009980:Influenza A virus / Q000201:enzymology*; D012316:RNA Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors*; D012254:Ribavirin / Q000494:pharmacology; D013379:Substrate Specificity
D001386:Azides; D003854:Deoxyribonucleotides; D012254:Ribavirin; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase
Ingibirovanie aktivnosti RNK-polimerazy virusa grippa A nekotorymi 3'-amino-3'-dezoksi- i 3'-azido-3'-dezoksiribonukleozid-5'-tri- fosfatami.