Determination of kynurenine in serum by high-performance liquid chromatography after enzymatic conversion to 3-hydroxykynurenine.
A method for the determination of the physiological level of kynurenine in human serum based upon conversion of kynurenine to 3-hydroxykynurenine by enzymatic reaction with the mitochondrial fraction and NADPH and analysis by reversed-phase high-performance liquid chromatography with electrochemical detection is described. Tryptophan gave no interference. For one analysis, 0.2 ml of serum was sufficient, compared with the large volume (5.0 ml) required for other methods.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007737:Kynurenine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000097:blood*; D050601:Kynurenine 3-Monooxygenase; D008930:Mitochondria, Liver / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism
C005045:3-hydroxykynurenine; D007737:Kynurenine; D006899:Mixed Function Oxygenases; D050601:Kynurenine 3-Monooxygenase