Microinjection of normal cell extracts into Fanconi anemia fibroblasts corrects defective scheduled DNA synthesis recovery after 8-methoxypsoralen plus UVa treatment.
Fanconi anemia (FA) cells show an increased sensitivity to 8-methoxypsoralen (8-MOP) plus UVa treatment; after an initial reduction of their semiconservative DNA synthesis rate, they do not recover like normal cells. We microinjected extracts from normal cells into FA fibroblasts from complementation group A and determined semiconservative DNA synthesis rates by autoradiography; the hampered recovery phase was completely restored.
D000741:Anemia, Aplastic / Q000235:genetics*; D002478:Cells, Cultured; D002648:Child; D004249:DNA Damage; D004260:DNA Repair; D005199:Fanconi Anemia / Q000235:genetics*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008730:Methoxsalen / Q000494:pharmacology; D008845:Microinjections; D014466:Ultraviolet Rays
D008730:Methoxsalen