title: Determination of magnesium binding to macromolecules.
abstract: An equilibrium dialysis technique for examining magnesium binding to macromolecules is described. The technique is used to determine the binding constants of magnesium to human prothrombin. This procedure should be of great utility for many biochemical systems which exhibit magnesium affinity.
mesh_terms: D003956:Dialysis / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism*; D011485:Protein Binding; D011516:Prothrombin / Q000378:metabolism*
chemical_list: D011516:Prothrombin; D008274:Magnesium