Rapid separation of oligomers of polysialic acid by high-performance liquid chromatography.
A rapid procedure utilizing high-performance liquid chromatography was developed for the separation of homooligomers of sialic acid (N-acetylneuraminic acid). The method utilizes the anion exchanger Mono-Q HR 5/5 and can resolve sialyl oligomers with degrees of polymerization (DP) from 2 to 20 in 25 min. Previous methods required 1 to 9 days. Recoveries are quantitative and the method can be used either analytically to analyze the enzymatic digestion products of polysialic acid or semipreparatively to prepare sialyl oligomers of defined length. The method is potentially useful for analyzing other anionic oligosaccharides.
D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009844:Oligosaccharides / Q000302:isolation & purification*; D012794:Sialic Acids / Q000302:isolation & purification*
D009844:Oligosaccharides; D012794:Sialic Acids; C021319:polysialic acid