Stimulation by ethanol of triglyceride synthesis in fasted rat hepatocytes is dependent on the increase of glycerol 3-phosphate levels.
Ethanol was without effect on the incorporation of [U-14C]palmitate into triglycerides in hepatocytes from fed rats, but significantly increased triglyceride synthesis in hepatocytes from fasted rats. Glycerol alone induced a similar increase, but brought about a strong synergistic stimulation when added with ethanol. Glycerol 3-phosphate levels were also determined. It is concluded that only in the fasted state the ethanol-induced increase of glycerol 3-phosphate concentration becomes determinant for the stimulation of triglyceride synthesis from exogenous palmitate.
D000818:Animals; D002250:Carbon Radioisotopes; D000431:Ethanol / Q000494:pharmacology*; D005215:Fasting; D005994:Glycerophosphates / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D005994:Glycerophosphates; D010169:Palmitic Acids; D014280:Triglycerides; D019308:Palmitic Acid; D000431:Ethanol; C029620:alpha-glycerophosphoric acid