Release of glucagon-like peptide 1 immunoreactivity from the perfused rat pancreas.
A reliable radioimmunoassay for glucagon-like peptide 1 (GLP-1) was developed with a detection limit of 12 pmol/l, which enabled us to detect the subtle change in concentrations of this peptide in the perfusate from the perfused rat pancreas. With this RIA, GLP-1 immunoreactivity (GLP-1 IR) was found to be secreted synchronously with glucagon immunoreactivity upon arginine stimulation from the perfused rat pancreas. Gel chromatographic analysis showed the presence of GLP-1 IR in the pancreatic extract which is eluted at the same position as synthetic GLP-1 (1-37). Moreover HPLC analysis confirmed the presence of GLP-1 IR, which eluted at exactly the same position as synthetic GLP-1, in the perfusate from pancreas perfusion upon stimulation of arginine. These results suggest that the pancreas stores and secrets GLP-1 IR concomitantly with glucagon as one of the cleavage products of their common precursor.
D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005934:Glucagon / Q000032:analysis / Q000378:metabolism*; D052216:Glucagon-Like Peptide 1; D008297:Male; D010179:Pancreas / Q000187:drug effects*; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D010477:Perfusion; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D010455:Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D001120:Arginine