Biological and chemical studies on overheated brewed coffee.
Vapour formed from overheated decaffeinated coffee was condensed and tested for mutagenicity using the Ames assay in Salmonella typhimurium strains TA98 and TA100. Vapour produced at 73 and 100 degrees C exhibited no mutagenicity. The basic fraction of vapour produced at 350 degrees C showed weak mutagenicity towards strains TA98 with metabolic activation. The chemical analysis of this fraction identified pyridines and pyrazines as the major constituents. None of the compounds identified in this fraction has been reported as mutagenic when tested in the Ames assay.
D002110:Caffeine; D005591:Chemical Fractionation; D003069:Coffee / Q000032:analysis / Q000633:toxicity*; D006358:Hot Temperature; D008752:Methylene Chloride; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis
D003069:Coffee; D009153:Mutagens; D002110:Caffeine; D008752:Methylene Chloride