Cytosolic calcium after carbon tetrachloride, 1,1-dichloroethylene, and phenylephrine exposure. Studies in rat hepatocytes with phosphorylase a and quin2.
Carbon tetrachloride (CCl4) and 1,1-dichloroethylene (DCE), both hepatotoxins, inhibit sequestration of Ca2+ by rat liver endoplasmic reticulum (ER) both in vivo and in vitro. It is possible that, as a result, cytosolic Ca2+ concentrations rise in liver cells. In experiments presented here, isolated hepatocytes were exposed to CCl4, DCE, and phenylephrine (PE), a non-hepatotoxic alpha 1-adrenergic agent that mobilizes Ca2+. Cytoplasmic Ca2+ concentrations were evaluated by two methods: indirectly by assaying the activity of glycogen phosphorylase a, and directly by monitoring the fluorescence of quin2. In primary hepatocyte cultures, CCl4, DCE, and PE exposure increased the activity of phosphorylase a at 5 min from 39 +/- 2 to 130 +/- 12, 80 +/- 13, and 97 +/- 10 nmoles PO4(3-)/mg protein/min respectively. In rat hepatocyte suspensions loaded with quin2 and exposed to CCl4, DCE, or PE, cytosolic Ca2+ concentrations were elevated within 20 sec to 0.83 +/- 0.13, 0.59 +/- 0.06 and 0.99 +/- 0.14 microM Ca2+ respectively. Basal Ca2+ levels in these cells averaged 0.25 +/- 0.03 microM. Thus, CCl4 and PE apparently increased cytosolic Ca2+ levels to approximately the same extent, whereas DCE was somewhat less effective. The durations of the effects of CCl4 and PE were examined further by determining their time courses of elevated phosphorylase a activity. In hepatocyte cultures, increased phosphorylase a activity persisted through at least 60 min following CCl4 exposure. In contrast, phosphorylase a activity returned to basal levels by 20 min after PE. Increases in cytoplasmic Ca2+ levels that are sustained rather than transient may distinguish these hepatotoxic chlorinated aliphatic hydrocarbons from non-toxic hormonal agents.
D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000032:analysis*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D003600:Cytosol / Q000032:analysis; D004000:Dichloroethylenes / Q000633:toxicity*; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000032:analysis / Q000187:drug effects*; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology*; D010762:Phosphorylase a / Q000032:analysis*; D006005:Phosphorylases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000634:Aminoquinolines; D004000:Dichloroethylenes; D006843:Hydrocarbons, Chlorinated; D008054:Lipid Peroxides; D010656:Phenylephrine; C029297:vinylidene chloride; D002251:Carbon Tetrachloride; D010762:Phosphorylase a; D006005:Phosphorylases; C035739:Quin2; D002118:Calcium