Synergism between eosinophil cationic protein and oxygen metabolites in killing of schistosomula of Schistosoma mansoni.
To study the cytotoxic reactions responsible for mediating eosinophil damage to schistosomula of Schistosoma mansoni, we have used cytoplasts (eosinophil or neutrophil vesicles devoid of granules and nuclei, with an intact oxidase in their plasma membrane) in combination with purified eosinophil cationic protein (ECP) or major basic protein (MBP) in a cytotoxicity test toward schistosomula. Suboptimal concentrations of ECP (10(-6) M) or MBP (10(-6) M) resulting in less than 10% killing were used in combination with cytoplasts. Cytoplasts alone in the presence of immune serum tested over a wide range of cytoplast:schistosomula ratios generated superoxide and hydrogen peroxide, but were unable to damage schistosomula. However, when a suboptimal ECP concentration (10(-6) M) was combined with neutroplasts or eosinoplasts, 43.9% +/- 8.5 (n = 7) and 24.7% +/- 9.8 (n = 3), respectively, of the schistosomula were killed. Oxygen metabolites were responsible for the synergism, because cytoplasts from a patient with chronic granulomatous disease were unable to act in synergy with ECP. In contrast to ECP, no synergism was found between cytoplasts and MBP (10(-6) to 2 X 10(-5)M). These results show that oxygen metabolites are important for the killing of schistosomula by lowering the concentration of ECP needed to inflict damage.
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000502:physiology; D003601:Cytotoxicity Tests, Immunologic; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000502:physiology*; D006105:Granulomatous Disease, Chronic / Q000276:immunology; D006801:Humans; D007814:Larva; D009504:Neutrophils / Q000502:physiology; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology*; D012260:Ribonucleases; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000254:growth & development
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D012260:Ribonucleases; D010100:Oxygen