Identification of subunits of acetylcholine receptor that interact with a cholesterol photoaffinity probe.
All four subunits of the acetylcholine receptor in membrane vesicles isolated from Torpedo californica have been labeled with [3H]cholesteryl diazoacetate. As this probe incorporates into lipid bilayers analogously to cholesterol, this result indicates that acetylcholine receptor interacts with cholesterol. This investigation also demonstrates that this probe is a useful reagent for studying the interaction of cholesterol with membrane proteins.
D000345:Affinity Labels; D000818:Animals; D001391:Azo Compounds; D002462:Cell Membrane / Q000648:ultrastructure; D002784:Cholesterol / Q000378:metabolism*; D002788:Cholesterol Esters; D003979:Diazonium Compounds; D046911:Macromolecular Substances; D008563:Membrane Lipids / Q000378:metabolism; D010782:Photolysis; D011487:Protein Conformation; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014101:Torpedo
D000345:Affinity Labels; D001391:Azo Compounds; D002788:Cholesterol Esters; D003979:Diazonium Compounds; D046911:Macromolecular Substances; D008563:Membrane Lipids; D011978:Receptors, Nicotinic; C052336:cholesteryl diazoacetate; D002784:Cholesterol