On the existence of an amphotericin B--sterol complex in lipid vesicles and in propanol-water systems.
The amphotericin B (AmB) - ergosterol complex, formed by interaction of the antibiotic with ergosterol-containing phospholipid vesicles, is associated with the lipid bilayer. It has been shown by circular dichroism studies that the AmB-ergosterol complex formed in water-propanol binary mixtures has a similar structure to that observed in phospholipid vesicles. A positive cooperativity is found for the interaction of AmB with ergosterol. The similar AmB-cholesterol complex is much less stable and rearranges rapidly to a different conformation.
D000433:1-Propanol; D000666:Amphotericin B; D002784:Cholesterol; D002942:Circular Dichroism; D004875:Ergosterol; D008081:Liposomes; D008954:Models, Biological; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D014867:Water
D008081:Liposomes; D010713:Phosphatidylcholines; D014867:Water; D000666:Amphotericin B; D000433:1-Propanol; D002784:Cholesterol; D004875:Ergosterol