[Isolation of viable human tumor cells and their characteristics during culturing in diffusion chambers].
Methodical approaches for obtaining the viable tumour cells from solid human tumours are developed. Combination of the short-term enzymatic treatment of the tumour tissue pieces and their gradual rubbing through the metal sieve with the decreasing pore sizes permitted obtaining a large number of isolated tumour cells with the high percentage of viability.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D012983:Soft Tissue Neoplasms / Q000473:pathology*
Poluchenie zhiznesposobnykh opukholevykh kletok cheloveka i ikh kharakteristika pri kul'tivirovanii v diffuzionnykh kamerakh.