title: Mitochondrial transmembrane potential and pH gradient during anoxia.
abstract: The effect of anoxia on the mitochondrial transmembrane potential and pH gradient was studied in a preparation of isolated hepatocytes. Transmembrane potential (delta psi) was calculated from the distribution of triphenylmethylphosphonium between the mitochondrial, cytosolic, and extracellular compartments, which were separated by digitonin fractionation and centrifugation. Mitochondrial and cytosolic pH values were calculated from the distribution of the weak acid, dimethadione, which was determined similarly. After 30 min anoxia, the magnitude of mitochondrial delta psi was decreased from -163 to -133 mV and the delta pH (mitochondria vs. cytoplasm) was essentially unchanged (aerobic, 0.78 +/- 0.08; anaerobic, 0.76 +/- 0.11). Thus the protonmotive force (delta p = delta psi-Z delta pH), is largely retained even in the absence of electron flow and ATP synthesis. Inhibitors of the ATP synthase (oligomycin), mitochondrial adenine nucleotide carrier (atractyloside), and glycolytic pathway (2-deoxy-D-glucose) do not affect the ability of the cell to maintain delta psi during anoxia. Therefore, the results indicate that retention of the protonmotive force is not due to utilization of ATP produced by glycolysis and suggest that mechanisms exist to preserve ion distribution during anoxia.
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002470:Cell Survival; D003600:Cytosol / Q000502:physiology; D004734:Energy Metabolism; D006019:Glycolysis; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000503:physiopathology*; D007425:Intracellular Membranes / Q000502:physiology; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000502:physiology*; D051381:Rats; D012235:Rhodamines; D013997:Time Factors
chemical_list: D012235:Rhodamines; D000255:Adenosine Triphosphate; C029773:rhodamine B