Gas chromatographic-mass spectrometric determination of macrolide antibiotics in beef and pork using single ion monitoring.
A gas chromatographic-mass spectrometric (GC-MS) method using single ion monitoring (SIM) is described for the determination of residual macrolide antibiotics, oleandomycin, kitasamycin, spiramycin and tylosin, in beef and pork. For GC-MS determination, oleandomycin is acid hydrolysed to desoleandomycin and acetylated, in the same way as erythromycin. However, for elution from a GC column, the carbon-carbon double bonds in the antibiotics must be hydrogenated to single bonds before acid hydrolysis. Kitasamycin and spiramycin are therefore converted into hydroforocidine acetate and tylosin into hydro-O-mycaminosyl tylonolide acetate, which are determined by GC-MS with SIM.
D000107:Acetylation; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel; D008401:Gas Chromatography-Mass Spectrometry; D006865:Hydrogenation; D006868:Hydrolysis; D008460:Meat / Q000032:analysis*; D013552:Swine
D000900:Anti-Bacterial Agents