The effects of ethanol methotrexate and diphenylhydantoin on [14C] leucine incorporation by human trophoblast cells cultured in vitro.
The effects of alcohol, diphenylhydantoin and methotrexate on protein synthesis by human trophoblast have been studied in vitro using a cell culture technique. None of the drugs depressed protein synthesis at pharmacological levels and for diphenylhydantoin there was evidence that the cellular accumulation of this drug was negligible.
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology*; D010672:Phenytoin / Q000494:pharmacology*; D014176:Protein Biosynthesis; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism*
D000431:Ethanol; D010672:Phenytoin; D007930:Leucine; D008727:Methotrexate