Enzymatic phosphorylation of macrolide antibiotics.
Five macrolide antibiotics (erythromycin A, 1; oleandomycin, 3a; tylosin, 4a; spiramycins, 5a; leucomycin A3, 6a) have been phosphorylated enzymatically using cell-free extracts derived from Streptomyces coelicolor UC 5240. The necessary cofactors and the rates of the conversion have been determined.
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004917:Erythromycin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007933:Leucomycins / Q000378:metabolism; D013058:Mass Spectrometry; D009711:Nucleotides / Q000378:metabolism; D009827:Oleandomycin / Q000378:metabolism; D010766:Phosphorylation; D013302:Streptomyces / Q000201:enzymology*; D015645:Tylosin
D000900:Anti-Bacterial Agents; D007933:Leucomycins; D009711:Nucleotides; D004917:Erythromycin; D009827:Oleandomycin; D015645:Tylosin