Evidence for existence of various homologues and analogues of platelet activating factor in a lipid extract of bovine brain.
Vasodepressor phospholipid with platelet-aggregating activity was highly purified from a lipid extract of bovine brain and subjected to field desorption-mass spectrometry. It was further analyzed by gas-liquid chromatography-mass spectrometry after hydrolysis with phospholipase C and conversion to tert-butyldimethylsilyl derivatives. Results indicated the presence of four species of platelet activating factor (1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine, PAF) and ten acyl analogues of PAF. The acyl analogues of PAF included species having an sn-2-propionyl or sn-2-butyryl group, which have not been previously detected in natural sources. The total amount of acyl analogues of PAF was much higher than that of PAF.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D008055:Lipids / Q000302:isolation & purification; D013058:Mass Spectrometry; D010743:Phospholipids / Q000302:isolation & purification; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000302:isolation & purification*
D008055:Lipids; D010743:Phospholipids; D010972:Platelet Activating Factor