Ultramicro-ELISA for calmodulin and anticalmodulin.
A non-competitive ELISA-technique for the determination of anticalmodulin as well as a sensitive competitive ELISA for calmodulin in 10 microliters samples has been developed. The assay for calmodulin has an inter-assay and intra-assay variability of 18% and 17%, respectively; the limit of detection is 20 pg per assay. The corresponding values for the assay of anticalmodulin are less than 6%, less than 6% and 35 pg, respectively. The methods for the purification of calmodulin and the production of anticalmodulin are also described.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002147:Calmodulin / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D008832:Microchemistry; D011817:Rabbits; D012756:Sheep; D013737:Testis / Q000032:analysis
D000906:Antibodies; D002147:Calmodulin