The effects of chemically modifying serum apolipoproteins on their ability to activate lipoprotein lipase.
Lipoprotein lipase activity was measured in an acetone-dried-powder preparation from rat epididymal adipose tissue using pig serum or pig serum lipoprotein, which had been chemically modified, as activator. Modification of acidic amino acids of lipoproteins with NN-dimethyl-1,3-diamine resulted in a complete loss of ability to activate lipoprotein lipase. Modification of 34% of lipoprotein arginine groups with cyclohexanedione resulted in the loss of 75% of the activation of lipoprotein lipase; approx. 42% of the original activity was recovered after reversal of the modification. This effect was dependent on the cyclohexanedione concentration. Modification of 48% of lipoprotein lysine groups with malonaldehyde decreased the maximum activation by 20%, but three times as much lipoprotein was required to achieve this. Non-enzymic glycosylation of lipoprotein with glucose, under a variety of conditions resulting in up to 28 nmol of glucose/mg of protein, had no effect upon the ability to activate lipoprotein lipase. In contrast non-enzymic sialylation resulted in a time-dependent loss of up to 60% of ability to activate lipoprotein lipase. Reductive methylation and acetoacetylation of serum did not affect the ability to activate lipoprotein lipase. The results are compared to the effects of similar modifications to low density lipoproteins on receptor-mediated endocytosis.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001053:Apolipoproteins / Q000097:blood / Q000494:pharmacology*; D003512:Cyclohexanones / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008315:Malondialdehyde; D008745:Methylation; D019158:N-Acetylneuraminic Acid; D051381:Rats; D012794:Sialic Acids / Q000494:pharmacology; D013552:Swine
D000596:Amino Acids; D001053:Apolipoproteins; D003512:Cyclohexanones; D012794:Sialic Acids; D008315:Malondialdehyde; D008071:Lipoprotein Lipase; D019158:N-Acetylneuraminic Acid; D005947:Glucose