[Determination of purine and pyrimidine derivatives in acid-soluble fractions of organs and tissues using high-performance reverse-phase ion-pair chromatography].
Simultaneous separation of the main ribonucleotides, nucleosides and bases was carried out using tetrabutyl ammonium phosphate (TBA) hetaerons with a reversed-phase (C18) packing material. Chromatographic properties of purine and pyrimidine metabolites in radially compressed NovaPak C18 system was studied at various concentrations of acetonitrile and TBA in the mobile phase. The optimal conditions for gradient separation of nucleotides, nucleosides and bases were evaluated. The procedure was used for analysis of blood from healthy persons and patients with acute myocardial infarction.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D051379:Mice; D009203:Myocardial Infarction / Q000097:blood; D011684:Purine Nucleosides / Q000032:analysis* / Q000097:blood; D011685:Purine Nucleotides / Q000032:analysis* / Q000097:blood; D011741:Pyrimidine Nucleosides / Q000032:analysis* / Q000097:blood; D011742:Pyrimidine Nucleotides / Q000032:analysis* / Q000097:blood; D013950:Thymus Gland / Q000032:analysis
D011684:Purine Nucleosides; D011685:Purine Nucleotides; D011741:Pyrimidine Nucleosides; D011742:Pyrimidine Nucleotides
Opredelenie purinovykh i pirimidinovykh proizvodnykh v sostave kislotorastvorimykh fraktsiĭ organov i tkaneĭ metodom vysokoéffektivnoĭ zhidkostnoĭ obrashchenno-faznoĭ ion-parnoĭ khromatografii.