title: Oxime-metabolizing activity of liver aldehyde oxidase.
abstract: Liver aldehyde oxidase in the presence of its electron donor exhibited a significant oxime-metabolizing activity toward some different types of oximes under anaerobic conditions. Acetophenone oxime and salicylaldoxime were exclusively converted to the corresponding oxo compounds, whereas benzamidoxime was converted to the corresponding ketimine. With d-camphor oxime, the formation of both the corresponding oxo compound and ketimine was observed. Stoichiometric studies showed that the formation of oxo compounds is accompanied by nearly equimolar ammonia. We propose a mechanism of oxime biotransformation that liver aldehyde oxidase catalyzes the reduction of oximes to the corresponding ketimines which in turn undergo, depending on their chemical stability, nonenzymatic hydrolysis to the corresponding oxo compounds and ammonia.
mesh_terms: D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000693:Anaerobiosis; D000818:Animals; D006224:Cricetinae; D003600:Cytosol / Q000201:enzymology; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D051379:Mice; D010091:Oximes / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine
chemical_list: D010091:Oximes; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase