title: Cross flow diafiltration of serum with basal medium suitable for growth of hybridomas, sterilization and protein reduction.
abstract: Fetal Calf Serum (FCS) was extensively extracted by cross flow diafiltration (Pellicon, Millipore) and sterilized using the basal growth medium (DMEM) for the extraction. Ultrafiltration membranes of 10(5) and 3 X 10(5) Dalton cut off were used respectively. The diafiltrates were used for hybridoma cultivation and the results of growth and mAb-production were compared with standard medium (DMEM + 5% FCS). Slightly reduced mAb-titers were achieved. These were, however, compensated by decreased concentration of contaminating protein and higher specific mAb/protein ratio as examined by SDS-PAGE and enzyme linked immuno electro transfer blot (EITB).
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D003956:Dialysis; D005374:Filtration; D006825:Hybridomas / Q000166:cytology*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media