title: Micro-ELISA for the quantitation of human urinary IgG.
abstract: When investigating glomerular changes in the early stages of diabetic renal disease, it is important to be able to estimate low concentrations of urinary immunoglobulins, as well as the albumin/immunoglobulin ratio. For this reason there is a need for highly sensitive and specific routine assays for urinary immunoglobulins. A 'sandwich' enzyme-linked immunoadsorbent assay (ELISA) for the quantitation of urinary immunoglobulin G is described, in which microtest plates are used as the solid phase. The assay is specific, sensitive and simple, and only used commercially available reagents. The assay range was 5-200 micrograms/l. The relative standard deviations within and between assays were 5 and 9%, respectively. Recovery of IgG added to urine was 100-102% (n = 12), and dilution of urine was linear. The assay range allowed for the quantitation of IgG in human urine samples, covering the clinical range from physiological to raised pathological values.
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine*
chemical_list: D007074:Immunoglobulin G