title: The NADPH consumption regulates the NADPH-producing pathways (pentose phosphate cycle and malic enzyme) in rat adipocytes.
abstract: The changes in the activity of the pentose phosphate cycle and the malic enzyme produced by the activation or inhibition of different NADPH-consuming pathways have been studied. The inhibition of the fatty acid synthesis by kynurenate produced a decrease in the flux through the pentose phosphate cycle and a diminution in the malic enzyme pathway. The incubation of the adipocytes in the presence of ter-butyl-hydroperoxide, a compound which is metabolized via a NADPH-consuming pathway, produced a big increase in the pentose phosphate cycle and the malic enzyme activities. The regulation of these NADPH-producing pathways by the NADPH/NADP ratio is discussed.
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002330:Carmustine / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D007736:Kynurenic Acid / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D020122:tert-Butylhydroperoxide
chemical_list: D010545:Peroxides; D009249:NADP; D020122:tert-Butylhydroperoxide; D008291:Malate Dehydrogenase; D007736:Kynurenic Acid; D002330:Carmustine