Polyploidization of G2M phase cells separated from aerobically and anaerobically grown Ehrlich ascites tumor cells.
G2-enriched fractions of Ehrlich ascites tumor cells (up to 80%-85% G2 cells) separated from anaerobically and aerobically cultured asynchronous populations by centrifugal elutriation revealed the same growth characteristics after recultivation under standard conditions: a significant proportion of cells with increased DNA (DNA content greater than 4C) emerged. Interruption of DNA synthesis by deprivation of oxygen may account for polyploidization (over-replication) of DNA but other mechanisms must be taken into consideration.
D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics*; D002455:Cell Division; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry
D004247:DNA