Evidence for a single common Na+-dependent transport system for alanine, glutamine, leucine and phenylalanine in brush-border membrane vesicles from bovine kidney.
The characteristics of the Na+-dependent transport of alanine, glutamine, leucine and phenylalanine were studied in bovine renal brush-border membrane vesicles. Inhibition of the transport of any one of these amino acids by any other was mutually competitive. The Ki value for the inhibition of alanine transport by leucine was similar to the Km for leucine transport; similar interrelationships existed for the other amino acids. Each amino acid was shown to exchange with each of the other amino acids across the membrane. From these and other results it is concluded that the Na+-dependent transport of these four amino acids is catalysed by a single common transport system.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D005973:Glutamine / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology*
D005973:Glutamine; D010649:Phenylalanine; D012964:Sodium; D007930:Leucine; D000409:Alanine