Effects of myosin light-chain kinase inhibitor on catecholamine secretion from rat pheochromocytoma PC12h cells.
Release of dopamine from rat pheochromocytoma PC12h cells by high K+ (50 mM) was inhibited by a specific inhibitor of myosin light-chain kinase (ML-9) dose-dependently. The myosin light-chain kinase inhibitor also specifically inhibited the phosphorylation of a 20 KDa protein by myosin light-chain kinase. Myosin light chain kinase may play a stimulatory role in the release reaction of catecholamines from the rat pheochromocytoma cells.
D000310:Adrenal Gland Neoplasms / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004298:Dopamine / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors* / Q000502:physiology; D010673:Pheochromocytoma / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats
D004791:Enzyme Inhibitors; D011493:Protein Kinase C; D009219:Myosin-Light-Chain Kinase; D004298:Dopamine