Characterization of the anticoagulants from Taiwan cobra (Naja naja atra) snake venom.
Taiwan cobra (Naja naja atra) snake venom was separated into 19 fractions by means of CM-Sephadex C-50 column chromatography. Anticoagulant Fractions V-VII were refractionated by gel filtration on Sephadex G-50 and the purified component possessed phospholipase A2 activity and an inhibitory effect on collagen-induced platelet aggregation. The anticoagulant action could be antagonized by phospholipid or platelet factor 3. Anticoagulant Fraction XVII was also further refractionated by gel filtration on Sephadex G-50 and the purified component was shown to be cardiotoxin. It was a weak anticoagulant, caused direct hemolysis and potentiated collagen-induced platelet aggregation. Thromboelastographic studies showed that the anticoagulant action of cobra venom is due to the synergistic effects of phospholipase A2 and cardiotoxin.
D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification* / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D002850:Chromatography, Gel; D004179:Cobra Cardiotoxin Proteins / Q000302:isolation & purification / Q000494:pharmacology; D004546:Elapid Venoms / Q000302:isolation & purification* / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D010741:Phospholipases A / Q000302:isolation & purification / Q000494:pharmacology; D054467:Phospholipases A2; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits
D000925:Anticoagulants; D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms; D010741:Phospholipases A; D054467:Phospholipases A2