Spectrin oxidation correlates with membrane vesiculation in stored RBCs.
An increase in spectrin oxidation in a variety of erythrocytes displaying a tendency to vesiculate has been previously described. To explore this relationship in more detail, we have studied blood stored in citrate-phosphate-dextrose-adenine under blood bank conditions because, in this system, vesiculation occurs slowly. Vesiculation was quantitated by measuring acetylcholinesterase release, and the extent of spectrin oxidation was detected by using thiol-disulfide exchange chromatography. A strong correlation (r = .92) was found between the extent of spectrin oxidation and vesiculation when blood from five donors was analyzed at weekly intervals during storage. This strongly suggests that spectrin oxidation plays a role in the formation of spectrin-free vesicles, thereby limiting the shelf life of stored blood.
D004910:Erythrocyte Membrane / Q000502:physiology* / Q000648:ultrastructure; D005089:Exocytosis; D006801:Humans; D010084:Oxidation-Reduction; D013049:Spectrin / Q000378:metabolism*
D013049:Spectrin