Chemical modification of enzymes with activated magnetic modifier.
An activated magnetic modifier, which could render biological materials magnetic property, was synthesized in following two steps: oxidation of ferrous ions (Fe2+) with hydrogen peroxide in the presence of alpha, omega-dicarboxymethylpoly(oxyethylene) (DCPEG) to obtain DCPEG-magnetite (Fe3O4); free carboxyl groups in the DCPEG-magnetite were activated with N-hydroxysuccinimide. By coupling the activated magnetic modifier to amino groups of lipase or L-asparaginase, magnetic enzymes were prepared. They dispersed stably not only in aqueous solution but also in organic solvents with high enzymic activities. Magnetic enzymes were readily recovered from reaction mixture in a magnetic field of 6000 Oe without loss of enzymic activity.
D001215:Asparaginase; D006861:Hydrogen Peroxide; D007501:Iron; D008049:Lipase; D008280:Magnetics; D011092:Polyethylene Glycols; D013388:Succinimides
D013388:Succinimides; C052889:alpha, omega-dicarboxymethylpoly(oxyethylene)-magnetite; D011092:Polyethylene Glycols; D006861:Hydrogen Peroxide; D007501:Iron; D008049:Lipase; D001215:Asparaginase; C001426:N-hydroxysuccinimide