Factors involved in the increase of K+ efflux of erythrocytes in low chloride media.
The rate constant of the Rb+(K+) efflux in isotonic NaCl solutions containing sucrose is increased when the NaCl concentration is reduced. When NaCl is replaced by Na-gluconate no change of the rate constant can be observed. The ionic strength of the solution is discussed as one factor influencing the Rb+(K+) efflux. The addition of furosemide (0.5 mM) inhibits the rate constant of the Rb+(K+) efflux in solutions of different ionic strength by about 50%. The increase of the rate constant in solutions of low ionic strength containing sucrose is interpreted as a stimulation of the chloride-dependent Na,K cotransport and the K,Cl cotransport.
D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009994:Osmolar Concentration; D011188:Potassium / Q000378:metabolism*; D012413:Rubidium / Q000097:blood; D012964:Sodium / Q000097:blood
D002712:Chlorides; D005665:Furosemide; D012964:Sodium; D012413:Rubidium; D005033:Ethylmaleimide; D011188:Potassium