[Carbohydrate determinants of organs of the reproductive tract of the mouse according to the results of using lectins with different specificities].
Histotopography of lectin receptor sites in adult mice ovary, oviduct, uterus, testis and epididymis has been investigated on light-optic level by means of lectin-peroxidase technique. Paraffin sections are treated with peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA) and Laburnum anagyroides lectin (LAL), conjugated with horseradish peroxidase. Concanavalin A (Con A) receptor sites are visualized by indirect method. The usefulness of lectins for selective histochemic evaluation of definite organ structures is demonstrated. Zona pellucida, luteocytes, oviductal and uterine epitheliocytes are rich in receptor sites for all lectin used in the investigation. The most intense binding to zonae pellucidae glycoconjugates possess WGA and LAL, to luteocytes--PNA, SBA and LAL, to oviductal and uterine epitheliocytes--Con A and LAL. The preferential SBA binding to the acrosomal system and plasma membranes of spermatogenic cells is demonstrated. Changes in lectin-binding patterns during the maturation of intraovarian oocytes and spermatogenic cells are also studied. The perspectives of practical application of the results obtained, as well as trends in further lectin histochemistry investigations of the reproductive system are discussed.
D000818:Animals; D050260:Carbohydrate Metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D005836:Genitalia, Female / Q000378:metabolism*; D005837:Genitalia, Male / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D011952:Receptors, Concanavalin A / Q000378:metabolism; D011975:Receptors, Mitogen / Q000378:metabolism*
D011952:Receptors, Concanavalin A; D011975:Receptors, Mitogen; C038223:peanut agglutinin receptor; C046772:soybean lectin receptor; C027503:wheat germ agglutinin receptor
Uglevodnye determinanty organov reproduktivnoĭ sistemy myshi po dannym ispol'zovaniia lektinov razlichnoĭ spetsifichnosti.