Endotoxin-like activity associated with Lyme disease Borrelia.
The newly recognized spirochete, Borrelia burgdorferi, the causative agent of Lyme Disease, has been examined for endotoxin-like activities as measured by the standard Farmacopea Ufficiale della Republica Italiana rabbit fever test and the Limulus amoebocyte lysate assay. The suspension of heat-killed microorganism caused a febrile response at a dose of 1 X 10(8) bacteria pro kilo. Similar results were obtained in the Limulus assay where the heat-killed spirochetes stimulated formation of solid clot until the concentration of 1 X 10(5) per ml. Both in pyrogen test and in Limulus assay heat-killed Escherichia coli exhibited a higher degree of potency. These results show that LD-Borrelia possess endotoxin-like activities which could help in understanding the pathogenesis of the clinical symptomatology of the disease.
D000818:Animals; D001898:Borrelia / Q000472:pathogenicity*; D004731:Endotoxins / Q000032:analysis*; D005334:Fever / Q000209:etiology; D008033:Limulus Test; D008193:Lyme Disease / Q000382:microbiology; D011817:Rabbits; D013987:Ticks / Q000382:microbiology
D004731:Endotoxins