title: Production of antibodies and development of enzyme immunoassay for determination of monensin in biological samples.
abstract: Monensin is converted to monensin bromoacetate, which provides successful coupling to protein and allows production of monensin-specific rabbit antisera. A modified indirect enzyme-linked immunosorbent assay (ELISA) was developed, which is highly sensitive (2 ng/mL) to monensin determination. Monensin recovery was 53-81% at 10 ppb in sera or urine, and 81-130% at 100 ppb in dichloromethane-extracted feces or water. Overall recovery was 98.8% with coefficients of variation from 6 to 52% over the range of monensin concentrations studied. This is the first immunoassay reported for the carboxylic ionophore antibiotics.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses / Q000276:immunology; D006801:Humans; D007202:Indicators and Reagents; D008985:Monensin / Q000032:analysis* / Q000276:immunology; D011506:Proteins / Q000032:analysis
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D008985:Monensin