Production of human beta-interferon in mouse L-cells.
Mouse L-cells, producing glycosilated human beta-interferon constitutively, were cultivated on microcarriers in bioreactors, equipped with a bubble-free aeration system up to 22 1 scale. Suitable aeration membrane surfaces and medium exchange rates permitted cultivation up to 8 X 10(6) cells/ml with a specific beta-interferon productivity of 2 X 10(4) U/10(6) cells/day. A correlation was found between the number of cell layers on the microcarriers and the specific human beta-interferon productivity.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002448:Cell Adhesion; D004274:DNA, Recombinant; D005285:Fermentation; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D010100:Oxygen / Q000378:metabolism
D000596:Amino Acids; D004274:DNA, Recombinant; D007370:Interferon Type I; D010100:Oxygen