A free radical scavenger role for daunomycin: inhibition of nonenzymatic oxidation of NADH.
The effect of daunomycin(Dm) on the nonenzymatic oxidation of NADH in the presence of metal ions, H2O2 and various ligands was examined. The results show that daunomycin inhibits the oxidation of NADH by reacting with one or more of the reaction intermediates which include OH., HO2., O2. and NAD.. This reaction is a reductive one involving the anthraquinone moiety of daunomycin as confirmed by control experiments by comparative UV-Vis analysis of the spectral character of daunomycin after reduction by NaBH4. Daunomycin, once reduced, apparently does not transfer its electron to another species such as O2 and is thus thought to play the role of free radical scavenger.
D004247:DNA / Q000187:drug effects; D003630:Daunorubicin / Q000494:pharmacology; D004579:Electron Transport; D005609:Free Radicals; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D008670:Metals; D009243:NAD; D010084:Oxidation-Reduction
D005609:Free Radicals; D008670:Metals; D009243:NAD; D004247:DNA; D006861:Hydrogen Peroxide; D003630:Daunorubicin