Purification of a young age-related glycoprotein (Ugl-Y) from normal human urine.
Ugl-Y is a glycoprotein that is detected in normal urine samples from young men and women aged 0 to 17 years. It was purified by ammonium sulfate precipitation and various column chromatographies including affinity chromatography using anti-adult urine antibody coupled to Sepharose 4B. The homogeneity of the glycoprotein was confirmed by polyacrylamide gel electrophoresis, isoelectric focusing, column chromatography on Sephadex G-75, and the precipitation reaction with anti-Ugl-Y antibody. It was shown to have a molecular weight of 29,000 by gel filtration, and to contain 5.2% neutral sugars (mannose and galactose) and 4% hexosamine (glucosamine). Amino acid analysis of the glycoprotein indicated high contents of acidic and hydroxylic amino acids. Its origin is unknown.
D000375:Aging / Q000652:urine*; D000596:Amino Acids / Q000032:analysis; D002648:Child; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D007120:Immunochemistry; D008297:Male; D008970:Molecular Weight
D000596:Amino Acids; D006023:Glycoproteins; C052642:young age-related glycoprotein, human