Comparison of basal and noradrenaline stimulated methylation of chloroform-extractable products in synaptosomal preparations from the rat brain.
Noradrenaline addition increases (S-adenosyl-L-methionine)-methylation of chloroform extractable products in the rat brain synaptosomal preparation. The characteristics of this methylation (MgCl2 dependence, S-adenosyl-L-methionine concentration dependence) are different from those of phosphatidylethanolamine methylase. The greatest increase was induced by noradrenaline in the 10(-4) M concentration range; preincubation of noradrenaline with the membranes, before S-adenosyl-L-methionine addition, was necessary. The TLC analysis of chloroform extractable products showed that: methylation of phosphatidylethanolamine derivatives was increased and the production of another unknown product (corresponding to that described by Wazer et al., Life Sci. 32, 2535, 1983) was more enhanced.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002251:Carbon Tetrachloride; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008745:Methylation; D009638:Norepinephrine / Q000494:pharmacology*; D010714:Phosphatidylethanolamines / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D012436:S-Adenosylmethionine / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*
D010714:Phosphatidylethanolamines; D015636:Magnesium Chloride; D012436:S-Adenosylmethionine; D002251:Carbon Tetrachloride; D008274:Magnesium; D009638:Norepinephrine