Synthesis and secretion of plasma cholesteryl ester transfer protein by human hepatocarcinoma cell line, HepG2.
We have examined the synthesis, secretion, and functional and physical characteristics of a lipid transfer protein synthesized by a human hepatocellular carcinoma line. We found that this protein shares immunochemical determinants and many other properties with the lipid transfer protein, LTP-I, which has been purified from human plasma. We conclude that the human liver cell line, HepG2, synthesizes and secretes LTP-I. Thus, hepatocytes may be the source of LTP-I in human plasma.
D000818:Animals; D001054:Apolipoproteins A / Q000378:metabolism; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D002460:Cell Line; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D006023:Glycoproteins; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007526:Isoelectric Point; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D001054:Apolipoproteins A; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters; D006023:Glycoproteins; D007106:Immune Sera; D003513:Cycloheximide