Cyanide is a product of the catalase-mediated oxidation of the alcohol deterrent agent, cyanamide.
Cyanide was detected as a product of cyanamide oxidation by bovine liver catalase in vitro under conditions that also produced an active aldehyde dehydrogenase (AlDH) inhibitor. Cyanide formation was directly related to both cyanamide and catalase concentrations and was also dependent on incubation time. The apparent Km for this reaction was 172 microM. Cyanide formation was blocked by ethanol, a known substrate for catalase Compound I. The toxic effects of cyanamide in the dog, a species with limited capacity to conjugate cyanamide by N-acetylation, may be causally related to enhancement of this catalase-mediated pathway for cyanamide metabolism.
D000427:Alcohol Deterrents / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002417:Cattle; D003484:Cyanamide / Q000378:metabolism*; D003486:Cyanides / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D010084:Oxidation-Reduction
D000427:Alcohol Deterrents; D003486:Cyanides; D000431:Ethanol; D003484:Cyanamide; D005949:Glucose Oxidase; D002374:Catalase; D005947:Glucose