Quantification by fluorescence of apurinic sites in DNA.
Time dependent fluorescence is observed when single or double stranded DNA with apurinic sites are mixed with 9-NH2-ellipticine. A concentration dependent plateau is obtained which is linearly related to the ratio of apurinic sites in DNA. We therefore suggest that it is possible to have a direct measurement of apurinic sites in DNA by fluorescence.
D000470:Alkaloids; D000818:Animals; D001080:Apurinic Acid / Q000032:analysis*; D002417:Cattle; D004247:DNA / Q000032:analysis*; D004249:DNA Damage; D004611:Ellipticines; D066298:In Vitro Techniques; D011119:Polynucleotides / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D000470:Alkaloids; D004611:Ellipticines; D011119:Polynucleotides; D001080:Apurinic Acid; C038373:9-aminoellipticine; D004247:DNA