Relationship between the uptake and cytotoxicity of celiptium in wild type and resistant mutants of the bacterium Streptococcus pneumoniae.
Celiptium, a cationic and amphiphilic drug currently employed in cancer chemotherapy, was found to be accumulated against its concentration gradient by the bacterium Streptococcus pneumoniae. Accumulation was reduced in Celiptium resistant amiA mutants which were also observed to have reduced electric transmembrane potentials delta psi. This suggested a relationship between Celiptium toxicity and accumulation in S. pneumoniae, and indicated a delta psi - driven uptake in a manner reminiscent of that observed for other lipophilic cations such as tetraphenylphosphonium.
D000470:Alkaloids / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004611:Ellipticines / Q000378:metabolism*; D008564:Membrane Potentials; D008727:Methotrexate / Q000378:metabolism; D009154:Mutation; D009861:Onium Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D000470:Alkaloids; D004611:Ellipticines; D009861:Onium Compounds; D009943:Organophosphorus Compounds; C027767:elliptinium; C013289:tetraphenylphosphonium; D008727:Methotrexate