[Effect of alpha-thrombin on DNA synthesis and excretion by lymphocytes].
Thrombin-activated spleen lymphocytes of mice synthesize 1.5 times more DNA than unstimulated cells. The proportions of palindromic sequences in the newly-synthesized nuclear DNA of control and thrombin-stimulated lymphocytes are 1.1% and 0.5%, respectively. Both the control and thrombin-stimulated lymphocytes release about 10-12% of the newly-synthesized cellular DNA into the culture medium. It is shown that the newly-synthesized DNA of viable, rather than non-viable cells, is the source of extracellular DNA.
D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis* / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007942:Leukemia, Experimental / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology*
D004273:DNA, Neoplasm; D004247:DNA; D013917:Thrombin
Vliianie alpha-trombina na sintez i ékskretsiiu DNK limfotsitami.