title: Oxidative phosphorylation of creatine by respiring pig heart mitochondria in the absence of added adenine nucleotides.
abstract: Isolated pig heart mitochondria were found to form phosphocreatine continuously at the rate of 12.5 +/- 1.8 nmol per min per mg of the mitochondrial protein in the respiration medium without externally added adenine nucleotides, and its formation rate showed a concentration dependency with respect to creatine and phosphate. The synthesis of phosphocreatine was completely inhibited by antimycin, FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), and atractyloside. However, oligomycin had no effect on the rate of phosphocreatine formation. These results are discussed in terms of a model that heart mitochondrial creatine kinase is functionally coupled to the oxidative phosphorylating system via the action of the adenine nucleotide translocase.
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D003401:Creatine / Q000378:metabolism*; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010725:Phosphocreatine / Q000378:metabolism*; D013552:Swine
chemical_list: D000227:Adenine Nucleotides; D009840:Oligomycins; D010725:Phosphocreatine; D003401:Creatine