Changes in N-acetyl-B-D-glucosaminidase and B-glucuronidase activities in milk during bovine mastitis.
To determine the N-acetyl-B-D-glucosaminidase (NAGase) and B-glucuronidase (B-Gase) activities in mastitic milk, basic enzyme assay conditions, distribution of NAGase and B-Gase, comparison of their activities with California Mastitis Test scores, and the effects of the milking process on their enzyme activities were examined. The mean NAGase and B-Gase activities in milk macrophages were about threefold higher than those of milk and blood polymorphonuclear cells. Very little NAGase activity appeared to be associated with blood mononuclear cells, whereas a relatively higher B-Gase activity was observed. California Mastitis Test scores of each group (1 to 5) appeared to be well correlated (r = 0.86 for NAGase and 0.92 for B-Gase) with the levels of NAGase and B-Gase activity. The milking process was least effective in the normal milk, but some variations of enzyme activities during milking in mastitic milk were found. Changes in NAGase and B-Gase activities in quarter milk were well monitored during the course of clinical mastitis.
D000118:Acetylglucosaminidase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005260:Female; D005966:Glucuronidase / Q000097:blood / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism*; D008214:Lymphocytes / Q000201:enzymology; D008264:Macrophages / Q000201:enzymology; D008414:Mastitis, Bovine / Q000201:enzymology*; D008892:Milk / Q000201:enzymology*; D009504:Neutrophils / Q000201:enzymology
D006596:Hexosaminidases; D005966:Glucuronidase; D000118:Acetylglucosaminidase