Preparation of 4,15-diacetoxyscirpenol from cultures of Fusarium sambucinum NRRL 13495.
Filtrates of Fusarium sambucinum NRRL 13495 grown in a stagnant culture for 9 days contained up to 458 +/- 60 (mean +/- standard error; n = 3) mg of 4,15-diacetoxyscirpenol per liter depending on culture conditions. Extraction with ethyl acetate, chromatography on a column of silica gel, and crystallization from mixtures of ethyl acetate and hexane provided pure material in 96% yield.
D000085:Acetates; D002855:Chromatography, Thin Layer; D005670:Fusarium / Q000378:metabolism*; D012717:Sesquiterpenes / Q000302:isolation & purification*; D014255:Trichothecenes / Q000096:biosynthesis / Q000302:isolation & purification*
D000085:Acetates; D012717:Sesquiterpenes; D014255:Trichothecenes; C007650:ethyl acetate; C001809:diacetoxyscirpenol