Incidence of sperm-associated immunoglobulins in infertile men with suspected autoimmunity to sperm.
Semen samples from 120 infertile men with suspected autoimmunity to sperm were investigated by a direct immunobead test (IBT). Fifty-three (44%) of them had 10% or more motile sperm coated with anti-IgG and/or anti-IgA immunobeads. Both classes of immunoglobulins were found to be present in 88.7% of the antibody positive ejaculates. These sperm-bound Igs were associated with sperm autoagglutination in 80% of the ejaculates and with decreased sperm penetration into cervical mucus in 97.6% of the cases. The close correlation found between the IBT results and the occurrence of antisperm antibodies in serum and in seminal plasma suggests that sperm-bound Ig's are sperm-specific antibodies. It is concluded that the direct IBT is not only a reliable screening test for sperm antibodies but is also a relevant test to determine whether these antibodies exert an influence on male fertility.
D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007159:Immunologic Tests; D007248:Infertility, Male / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013073:Sperm Agglutination; D013094:Spermatozoa / Q000276:immunology* / Q000502:physiology
D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G