Use of a dynamic filtration method for separation of animal cells.
For solving the problem of concentrating and washing of viable cell suspensions a dynamic filter device has been evaluated. Results show that concentrating of cells under sterile conditions can be performed. With a laboratory scale filter (Sulzer-Biodruckfilter BDF-01) a 9-fold concentration was achieved, the filtration speed was about 15 l/h. The viability did not change significantly. Details of experiments are given and advantages are discussed.
D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured / Q000502:physiology*; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D051379:Mice; D012399:Rotation