Allergenic activity of short ragweed pollen (Ambrosia elatior) from different years and/or suppliers: criteria for the selection of an in-house allergen reference preparation.
We have investigated dialyzed extracts (A-F) of short ragweed pollens (Ambrosia elatior) from six consecutive production years and four different companies by protein nitrogen units, high performance liquid chromatography, isoelectric focusing, SDS-PAGE, RAST inhibition, crossed radioimmunoelectrophoresis, and antigen E contents. Extract A showed lowest yields in all quantitative assays. Isoelectric focusing, SDS-PAGE, crossed immunoelectrophoresis, and crossed radioimmunoelectrophoresis showed comparable patterns for all extracts except for extract A. Apparently, the activity of short ragweed pollens depends on the year of production and/or the skill of the producer. These findings are in contrast to similar examinations of other pollen species.
D000485:Allergens / Q000032:analysis / Q000592:standards; D002241:Carbohydrates / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D011058:Pollen / Q000032:analysis / Q000276:immunology*; D011506:Proteins / Q000032:analysis; D012015:Reference Standards; D013997:Time Factors
D000485:Allergens; D002241:Carbohydrates; D011506:Proteins; D009584:Nitrogen