Effect of succinate on mitochondrial lipid peroxidation. 1. Comparative studies on ferrous ion and ADP . Fe/NADPH-induced peroxidation.
Lipid peroxidation in isolated rat liver mitochondria, mitoplast, and mitochondrial inner membrane fragments was induced either by ferrous ions, or in an NADPH-dependent process by complexing with adenine nucleotides (ADP or ATP) iron. The Fe2+-induced lipid peroxidation is nonenzymic when inner membrane fragments are used, while the differences in the inhibitory effect of Mn2+ ions and the stimulatory effect of the ionophore A-23187 in mitochondria and inner membrane fragments suggest an enzymic mechanism for ferrous ion-induced lipid peroxidation in intact mitochondria. Contrary to this the ADP/Fe/NADPH-dependent lipid peroxidation is an enzymic process both in mitochondria and inner membrane preparations. We have shown that cytochrome P450 is involved in the ADP/Fe/NADPH-induced lipid peroxidation. Succinate, a known inhibitor of NADPH-dependent lipid peroxidation, inhibited the Fe2+-induced process also, and there was no difference in this effect when inner membrane preparations, mitochondria, or mitoplasts were used.
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D001696:Biomechanical Phenomena; D005296:Ferrous Compounds / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013386:Succinates / Q000494:pharmacology*; D019802:Succinic Acid
D005296:Ferrous Compounds; D008054:Lipid Peroxides; D013386:Succinates; D009249:NADP; D000244:Adenosine Diphosphate; D019802:Succinic Acid