[Assessment and value of the polybrene microplate test for study and identification of irregular anti-erythrocyte antibodies].
We have adapted Lalezari's manual polybrene test for use with microplate technology for screening and identification of anti-erythrocytes antibodies with a view to future automation. The technical conditions have been standardized, firstly by using a programmable centrifuge and a sequential shaking, secondly by using a preservative medium for panel after dispensing onto microplates. This methodology has been run in parallel with papain test and LIS indirect antiglobulin test: 7,000 screenings have been performed and their results are considered here. Our results are comparable to those described for automatic and manual techniques. The polybrene-microplate test affords a fast, reliable, handy and inexpensive means of screening and identification for irregular antibodies. It appears as an additional method for enzymatic tests in microplate. An antiglobulin test can be carried out after negative tests.
D000906:Antibodies / Q000032:analysis*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology*; D006583:Hexadimethrine Bromide; D006801:Humans; D011073:Polyamines
D000906:Antibodies; D011073:Polyamines; D006583:Hexadimethrine Bromide
Evaluation et intérêt du test au polybrène en microplaque pour la recherche et l'identification des anticorps irréguliers anti-érythrocytes.