H-2-specific antibodies induced by injection of syngeneic leukemia cells.
AKR/J mice immunized with several syngeneic leukemia cells contained antibodies in their sera which reacted with certain AKR leukemia cell lines, depending on their H-2 expression, and precipitated H-2K antigens from lysates of leukemia cells. Precipitation of H-2K was not due to virus-specific antibodies: it could not be blocked by prior absorption with H-2-negative leukemias, but was blocked by certain allogeneic lymphocytes. Tumor-specific H-2K antibodies did not react with H-2K from normal AKR lymphocytes either on the cell surface or after detergent solubilization; however, they did react with H-2K from mitogen-activated AKR and BALB.K lymphoblasts. Since both these latter cells were also lysed by AKR-Gross/MuLV-specific and H-2Kk-restricted cytotoxic T lymphocytes, we consider the possibility that antibodies detecting conformational alterations induced in H-2Kk molecules by viral association may be present in syngeneic AKR antileukemia sera.
D000406:AKR murine leukemia virus; D000818:Animals; D006183:H-2 Antigens / Q000276:immunology*; D007518:Isoantibodies / Q000096:biosynthesis*; D007942:Leukemia, Experimental / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008934:Mitogens / Q000494:pharmacology; D009368:Neoplasm Transplantation; D014185:Transplantation, Isogeneic
D006183:H-2 Antigens; D007518:Isoantibodies; D008934:Mitogens