An isoelectric focusing study of acid phosphatase heterogeneity in rat liver.
The different forms of acid phosphatase (EC 3.1.3.2) in rat liver homogenates, lysosomal, mitochondrial, microsomal fractions and cytosol were studied with isoelectric focusing. Evidence is presented that isoelectric focusing of acid phosphatase in subcellular fractions shows individual changes and time related patterns. Mild autolysis shifted all enzyme activity peaks of isoelectric focusing patterns to the one at pH 7.04.
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism; D002940:Circadian Rhythm; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D051381:Rats
D007527:Isoenzymes; D000135:Acid Phosphatase; D001192:Arylsulfatases