Immunochemical similarity between a gastric mucosa non-pepsin acid proteinase and neutrophil cathepsin E of the rat.
Antiserum against a rat gastric mucosa non-pepsin acid proteinase precipitates rat neutrophil cathepsin E, with a precipitation curve essentially similar to that of the gastric enzyme. Taken together that the antiserum precipitates a cathepsin E-like acid proteinase from rat spleen (Muto, N., Yamamoto, M. and Tani, S. (1987) J. Biochem. (Tokyo) in press), the data indicate that the non-cathepsin D acid proteinases in rat neutrophils, gastric mucosa and spleen are immunochemically closely related. In contrast with the earlier data, cathepsin E from rabbit neutrophils exhibited a maximal activity at around pH 3.0-3.2 and preferred hemoglobin to albumin as substrate, which supports that the non-cathepsin D acid proteinases in the rat tissues are relevantly classified as cathepsin E.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D020059:Cathepsin E; D002403:Cathepsins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D005753:Gastric Mucosa / Q000201:enzymology*; D007700:Kinetics; D009504:Neutrophils / Q000201:enzymology*; D051381:Rats
D000906:Antibodies; D000936:Antigen-Antibody Complex; D002403:Cathepsins; D020059:Cathepsin E