title: Structural elements of bactopterin from Pseudomonas carboxydoflava carbon monoxide dehydrogenase.
abstract: Bactopterin is a novel pterin occurring in bacterial molybdoenzymes as the organic portion of the molybdenum cofactor. Its structure is investigated here. The compound contains a single pterin ring and carries a side chain at carbon atom 6 of the pterin nucleus as indicated by the formation of pterin-6-carboxylic acid upon alkaline permanganate oxidation. Studies with phosphate-cleaving enzymes revealed the presence of two monophosphoric acid monoesters. The affinity of reduced bactopterin for thiol-Sepharose points to the presence of thiol(s) in active bactopterin.
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003067:Coenzymes; D003597:Cytosine Nucleotides; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D009097:Multienzyme Complexes; D010084:Oxidation-Reduction; D010710:Phosphates / Q000032:analysis; D011549:Pseudomonas / Q000201:enzymology*; D011621:Pteridines; D011622:Pterins / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D000445:Aldehyde Oxidoreductases; D003067:Coenzymes; D003597:Cytosine Nucleotides; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D009097:Multienzyme Complexes; D010710:Phosphates; D011621:Pteridines; D011622:Pterins; D013438:Sulfhydryl Compounds; C066895:molybdopterin cytosine dinucleotide; C022261:molybdopterin; C019823:carbon monoxide dehydrogenase