Optimal parameters for in vitro growth of parvoviruses.
A procedure for optimal production or isolation of parvoviruses was found to be as follows: Indicator cells were infected in suspension using a cell concentration associated with full monolayer not before 3-4 days. The infected monolayer was passaged at similar cell concentrations, and the procedure repeated for up to 28 days culture of samples in this way. The optimal serum supplement used was free of or had low haemagglutination inhibitory activity to the virus studied and was used at minimal concentrations. Alternatively it was possible to remove serum supplement inhibitory activity by treatment with MnCl2/heparin dialysed and sterilized before use as serum supplement.
D000818:Animals; D001769:Blood; D002415:Cats; D002478:Cells, Cultured; D002712:Chlorides; D003470:Culture Media; D004285:Dogs; D006388:Hemagglutinins / Q000096:biosynthesis; D006493:Heparin / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D017895:Manganese Compounds; D010321:Parvoviridae / Q000254:growth & development* / Q000302:isolation & purification; D011309:Preservation, Biological
D002712:Chlorides; D003470:Culture Media; D006388:Hemagglutinins; D017895:Manganese Compounds; D008345:Manganese; D006493:Heparin; C025340:manganese chloride