title: 99mTc bone scanning agents--V. Influence of experimental conditions on the labeling efficiency and gel chromatography of 99mTc(Sn)HMDP.
abstract: The preparation of 99mTc(Sn)HMDP was investigated as a function of pH, Sn(II) and ligand concentration. HMDP could be labeled efficiently from pH 2-9. The Sn(II) and the ligand concentrations had a beneficial influence. The composition of the radiopharmaceutical under various experimental conditions was studied by means of gel chromatography on Biogel P-4. Six different complexes were found. A preparation consisted of maximally three major complexes. The presence of a particular complex was mainly determined by pH and ligand concentration. The Sn(II) concentration had little influence.
mesh_terms: D001842:Bone and Bones / Q000000981:diagnostic imaging*; D002850:Chromatography, Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011877:Radionuclide Imaging; D013669:Technetium Tc 99m Medronate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification
chemical_list: C052435:technetium Tc 99m (Sn) hydroxymethylene diphosphonate; D013669:Technetium Tc 99m Medronate