title: Cutaneous hyperalgesia: contributions of the peripheral and central nervous systems to the increase in pain sensitivity after injury.
abstract: This study assesses the contributions of the peripheral and central nervous systems in the development of hyperalgesia (increased pain sensitivity) after an injury. Experiments were carried out to examine the role of C-fiber afferents, the spinal cord and sympathetic efferents on inflammation, primary hyperalgesia and referred hyperalgesia produced in rats by a heat injury. A peripheral mechanism was indicated since both primary hyperalgesia and inflammation after a heat injury were significantly attenuated by blocking C-fiber afferents with local capsaicin. In addition, a central mechanism was indicated since the spread of hyperalgesia to the paw contralateral to a heat injury was prevented by either spinal anesthesia or the blocking of sympathetic efferents by guanethidine. A further role for central mechanisms was indicated since referred hyperalgesia--the enhancement of self-mutilation (autotomy) of a denervated limb which had previously sustained a heat injury--was reduced by spinal anesthesia or a combined blocking of C-fiber afferents and sympathetic efferents with intrathecal capsaicin + guanethidine. The results strongly suggest that referred hyperalgesia after a heat injury is dependent on increased spinal cord activity. However, autotomy in rats that did not undergo a previous injury was unaffected by either spinal anesthesia or intrathecal capsaicin. This suggests that spinal cord hyperactivity, although it plays a role in hyperalgesia following a heat injury, is not a crucial factor in producing pain and hyperalgesia after a nerve injury.
mesh_terms: D000818:Animals; D002056:Burns / Q000150:complications*; D002211:Capsaicin / Q000494:pharmacology; D002490:Central Nervous System / Q000503:physiopathology*; D006145:Guanethidine / Q000494:pharmacology; D006930:Hyperalgesia / Q000209:etiology*; D006941:Hyperesthesia / Q000209:etiology*; D008297:Male; D009407:Nerve Block; D009412:Nerve Fibers / Q000503:physiopathology; D010146:Pain; D010525:Peripheral Nerves / Q000503:physiopathology*; D011343:Procaine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011930:Reaction Time; D012652:Self Mutilation; D012867:Skin / Q000293:injuries*
chemical_list: D011343:Procaine; D002211:Capsaicin; D006145:Guanethidine