Synthesis of the trypsin fragment 10-25/75-88 of mouse nerve growth factor. I. The protected tetradecapeptide 75-88.
Cyanogen bromide cleavage followed by trypsin digestion of mouse nerve growth factor (NGF) allowed the isolation of a double chain unsymmetrical cystine peptide of high neurotrophic activity. The presence of tryptophan residues severely limits the synthetic approaches for selective disulfide bridging. The strategy applied for such a purpose as well as the preparation of the suitably protected tetradecapeptide corresponding to sequence 75-88 as key intermediate for the synthesis of the NGF fragment 10-25/75-88 are described.
D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide; D007202:Indicators and Reagents; D051379:Mice; D020932:Nerve Growth Factor; D009414:Nerve Growth Factors / Q000138:chemical synthesis*; D009904:Optical Rotation; D010446:Peptide Fragments / Q000138:chemical synthesis*; D014357:Trypsin
D007202:Indicators and Reagents; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D020932:Nerve Growth Factor; C051997:nerve growth factor (75-88); D014357:Trypsin; D003488:Cyanogen Bromide