Application of molecular-secondary-ion mass spectrometry for drug metabolism studies. I. Direct analysis of conjugates by thin-layer chromatography-secondary-ion mass spectrometry.
Molecular-secondary-ion mass spectrometry (SIMS) is a suitable method for the analysis of nonvolatile substances such as conjugated metabolites of drugs. We have developed a simple method for the direct SIMS measurement of conjugates following thin-layer chromatography without any extraction procedure. After separation with a butanol-acetic acid-ethanol-water (3:1:1:1, v/v) system, the spot was cut out and attached to a SIMS probe. The conjugates of p-nitrophenol and 4-hydroxyantipyrine were measured. The quantitative application of the method is also discussed, using deuterium-labelled internal standards for p-nitrophenol conjugates.
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives / Q000032:analysis / Q000652:urine; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D005965:Glucuronates / Q000032:analysis / Q000652:urine; D007275:Injections, Intravenous; D008297:Male; D013058:Mass Spectrometry; D009578:Nitrobenzenes / Q000032:analysis / Q000652:urine; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D005965:Glucuronates; D009578:Nitrobenzenes; D004364:Pharmaceutical Preparations; C002730:4-nitrophenylglucuronide; C023379:4-nitrophenyl sulfate; C007983:4-hydroxyantipyrine; D000983:Antipyrine