title: Circular dichroism study of bacteriorhodopsin-lipid interaction.
abstract: Delipidated bacteriorhodopsin purified from purple membrane of H. halobium was reconstituted with the circular dichroism active phospholipid. The observed circular dichroism spectra in the 450-700 nm region characteristic of bacteriorhodopsin showed the temperature dependence characterized by a midpoint at ca. 45 degrees C and this spectral change showed the disaggregation of bacteriorhodopsin trimer to monomer. The circular dichroism spectra in the 250-400 nm region characteristic of the azo chromophore of phospholipid exhibited a remarkable temperature dependence synchronized with the disaggregation of bacteriorhodopsin, suggesting that a large proportion of the phospholipid is present as boundary lipid.
mesh_terms: D001436:Bacteriorhodopsins / Q000378:metabolism*; D002942:Circular Dichroism; D006217:Halobacterium / Q000378:metabolism; D008081:Liposomes; D010713:Phosphatidylcholines / Q000378:metabolism*; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D001436:Bacteriorhodopsins