title: A rapid, objective method for the detection of lymphocytotoxic antibodies using flow cytometry.
abstract: Whilst several centres have reported lymphocytotoxic antibody detection using single and dual fluorescent stains with analysis of the fluorescence emitted from the cell population present in a well of a multiwell plate, problems are encountered with cell concentration and light emission overlap. A method we have developed using flow cytometry produces similar values of percentage cell death using single or double staining techniques (correlation coefficient = 0.9896). This method is not influenced by slight variation in cell number or light emission overlap. The effect of introducing red cell impurities into the normal lymphocyte preparation is described.
mesh_terms: D000961:Antilymphocyte Serum / Q000032:analysis*; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods*; D005452:Fluoresceins; D006801:Humans; D011419:Propidium
chemical_list: D000961:Antilymphocyte Serum; D005452:Fluoresceins; C027780:carboxyfluoresceindiacetate; D011419:Propidium