A theoretical analysis of the effects of sonication on differential absorption flattening in suspensions of membrane sheets.
A theoretical analysis is presented for the flattening of absorption and circular dichroic spectra of suspensions of membrane sheets containing proteins. Equations are presented to describe the dependence of this artifact on the size of the sheets. Values for the flattening coefficients QA and QB are calculated both as a function of absorptivity and sheet size. These studies show that sonication in an inadequate procedure for eliminating flattening in these samples.
D008433:Mathematics; D008565:Membrane Proteins / Q000502:physiology*; D008954:Models, Biological; D011487:Protein Conformation; D014465:Ultrasonics
D008565:Membrane Proteins