Anti-actin antibodies. Detection and quantitation of total and skeletal muscle actin in human plasma using a competitive ELISA.
A competitive ELISA has been used to titrate skeletal muscle and total actins in human plasma. Specific antibodies directed against the variable N-terminal 1-7 sequence and conserved sequences respectively were used. The N-terminus of actin appears to be accessible in native and brevin-complexed actins. The skeletal muscle actin isoform represents about 1% of the total circulating actin (mean: 50 micrograms/ml plasma), but is markedly increased after severe muscle tissue injuries.
D000199:Actins / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D000328:Adult; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D018260:Gelsolin; D006801:Humans; D008840:Microfilament Proteins; D008875:Middle Aged; D009132:Muscles / Q000276:immunology / Q000378:metabolism*; D014947:Wounds and Injuries / Q000097:blood
D000199:Actins; D000906:Antibodies; D002352:Carrier Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; C016953:brevin