Simple and sensitive determination of methylglyoxal in biological samples by gas chromatography with electron-capture detection.
Methylglyoxal was allowed to react with 4,5-dichloro-1,2-phenylenediamine, and the 6,7-dichloro-2-methylquinoxaline formed was determined by gas chromatography with electron-capture detection. The standard curve of the quinoxaline was linear up to 160 pmol/ml. The recoveries of methylglyoxal from coffee and rat liver homogenate were 84.1 and 77.6%, respectively. This procedure was very selective and so sensitive that as little as 9 fmol of the quinoxaline could be measured in biological and food samples.
D000447:Aldehydes / Q000032:analysis*; D000818:Animals; D002849:Chromatography, Gas; D003069:Coffee / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004563:Electrochemistry; D005215:Fasting; D008297:Male; D011765:Pyruvaldehyde / Q000032:analysis*; D011810:Quinoxalines / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014867:Water / Q000032:analysis
D000447:Aldehydes; D003069:Coffee; D011810:Quinoxalines; D014867:Water; D011765:Pyruvaldehyde