Bone formation in organ cultures of bone marrow.
Bone formation in organ cultures of intact marrow fragments from mouse is described. Marrow explants were cultured on the top surface of a millipore filter at a gas-liquid interface. Observations with both light- and electron microscopes demonstrated the formation of a well-organised trabecular matrix lined with osteoblast-like cells. The tissue and cells were positive for alkaline-phosphatase activity. Large amounts of thick, well-banded collagen fibrils and matrix vesicles typical of those found in bone were present. The tissue became mineralised in the presence of 10 mM Na-beta-glycerophosphate; in its absence a similar trabecular matrix developed but mineralisation did not take place.
D000818:Animals; D001846:Bone Development; D001853:Bone Marrow / Q000648:ultrastructure; D001854:Bone Marrow Cells; D003094:Collagen / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000166:cytology / Q000648:ultrastructure
D003094:Collagen