Study of the metabolism of pyrazinamide using a high-performance liquid chromatographic analysis of urine samples.
A reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of pyrazinamide and its metabolites in urine. Study of the metabolism of pyrazinamide by this method demonstrated that 5-hydroxypyrazinamide excretion was compatible with pyrazinoic acid excretion and allopurinol decreased in vivo conversion of pyrazinamide to 5-hydroxypyrazinamide and blocked that of pyrazinoic acid to 5-hydroxypyrazinoic acid.
D000493:Allopurinol / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011718:Pyrazinamide / Q000031:analogs & derivatives / Q000378:metabolism / Q000652:urine*; D014969:Xanthine Oxidase / Q000172:deficiency; D014970:Xanthines / Q000652:urine
D014970:Xanthines; C032191:5-hydroxypyrazinamide; D011718:Pyrazinamide; C005296:pyrazinoic acid; D000493:Allopurinol; D014969:Xanthine Oxidase