Induction of methamphetamine-specific antibody using biodegradable carboxymethyl-chitin.
Induction of a specific antibody for methamphetamine, an antihypnotic drug, has been studied using carboxymethyl-chitin (CM-chitin) as a hapten carrier. The hapten-specific antiserum was induced by only a couple of hypodermal injections every 2 weeks. Inhibition of antigen-antibody complex formation was linearly related to methamphetamine concentration in the range 0.5 to 50 ng/50 microliter when an enzyme-linked immunosorbent assay was performed using an avidin-biotin-peroxidase complex. Little antibody directed against CM-chitin and CM-chitin oligomer was detected. Thus it seems to be advantageous in the hapten-bovine serum albumin system. The specificity of the antibody was high as shown by the use of various methamphetamine analogs.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001673:Biodegradation, Environmental; D002686:Chitin / Q000031:analogs & derivatives* / Q000276:immunology; D003429:Cross Reactions; D006241:Haptens; D008297:Male; D008694:Methamphetamine / Q000031:analogs & derivatives / Q000276:immunology*; D011817:Rabbits
D006241:Haptens; D002686:Chitin; D008694:Methamphetamine; C044872:O-(carboxymethyl)chitin