title: Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum.
abstract: About 96% of the amino acid sequence of an alpha-tubulin from the slime mould Physarum polycephalum has been determined. Of 430 sequenced amino acids, 30 differ from the deduced amino acid sequence of a recently published alpha-tubulin complementary DNA from the plasmodial form of P. polycephalum. The myxamoebal alpha-tubulin differs from all other known alpha-tubulins in one of the last three C-terminal amino acids that are Gly-Glu-Tyr instead of the usual Glu-Glu-Tyr. These last three amino acids are preceded by 11 residues that appear to be particularly susceptible to mutation. No heterogeneity was found whilst sequencing the myxamoebal alpha-tubulin, indicating that only one type of alpha-tubulin is present in myxamoebae. This alpha-tubulin appears to be less conserved than the previously described plasmodial alpha-tubulin, supporting the hypothesis that the structural constraints on tubulin in axonemes have a significant effect on its rate of mutation.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D006801:Humans; D010804:Physarum / Q000032:analysis*; D051381:Rats; D012617:Sea Urchins; D013552:Swine; D014404:Tubulin
chemical_list: D014404:Tubulin