title: Correlation between the in vitro interaction of tumor cells with an organ environment and metastatic behavior in vivo.
abstract: The purpose of this study was to determine whether the in vitro interaction of tumor cells with an organ fragment correlated with organ colonization in vivo. Several clones of the K-1735 melanoma that exhibit different potentials for lung colonization or the heterogeneous M-5076 reticulum cell sarcoma which preferentially colonizes the liver, were used. The simultaneous subcutaneous implantation of a lung fragment with high lung-colonizing K-1735 cells stimulated the production of tumors, but this was not the case with the M-5076 cells, which do not colonize the lungs after intravenous injection. The ability of the tumor cells to survive in vitro in a lung fragment correlated with their capacity to produce lung tumor colonies after intravenous injection. Moreover, lung-conditioned medium stimulated the growth of lung-colonizing K-1735-M2 cells but not liver-colonizing M-5076 cells. Collectively, the data suggest that tissue-mediated growth stimulation may be one of the factors influencing the metastatic pattern and potential of tumor cells.
mesh_terms: D000818:Animals; D002450:Cell Communication; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D008168:Lung / Q000502:physiology; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation
chemical_list: D003470:Culture Media