title: Changes in the pattern of protein synthesis during zoospore germination in Blastocladiella emersonii.
abstract: Using two-dimensional gel electrophoresis, we analyzed the pattern of proteins synthesized during Blastocladiella emersonii zoospore germination in an inorganic solution, in both the presence and absence of actinomycin D. During the transition from zoospore to round cells (the first 25 min), essentially no qualitative differences were noticeable, indicating that the earliest stages of germination are entirely preprogrammed with stored RNA. Later in germination (after 25 min), however, changes in the pattern of protein synthesis were found. Some of these proteins (a total of 6 polypeptides) correspond possibly to a selective translation of stored messages, whereas the majority of the changed proteins (22 polypeptides) corresponds to newly synthesized mRNA. Thus, multiple levels of protein synthesis regulation seem to occur during zoospore germination, involving both transcriptional and translational controls. We also analyzed the pattern of protein synthesis during germination in a nutrient medium; synthesis of specific polypeptides occurred during late germination. During early germination posttranslational control was also observed, several labeled proteins from zoospores being specifically degraded or charge modified.
mesh_terms: D001754:Blastocladiella / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation; D008411:Chytridiomycota / Q000502:physiology*; D003609:Dactinomycin / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D013172:Spores, Fungal / Q000502:physiology*
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D003609:Dactinomycin