Human fibroblast-derived growth factor is a mitogen and chemoattractant for endothelial cells.
Human fibroblasts were found to produce a potent mitogen and chemoattractant for fetal bovine aortic endothelial cells. Homogenates from AG1523 and AG1518 foreskin, CCD18Lu lung, and CCD18Co colon fibroblasts produced half-maximal stimulation of endothelial cell growth at concentrations of 1-7 micrograms/ml. The factor was purified from large-scale cultures of the CCD18Co fibroblasts using cation exchange chromatography and heparin-Sepharose chromatography. Such preparations were mitogenic for endothelial cells in vitro at concentrations of about 5-10 ng/ml, and promoted chemotaxis at 0.1-1 ng/ml. Heparinase treatment of the cells prevented the chemotactic response. These properties suggest that the factor may be related to fibroblast growth factor.
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002633:Chemotaxis; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005333:Fetus; D005346:Fibroblast Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008297:Male; D008934:Mitogens
D008934:Mitogens; D005346:Fibroblast Growth Factors