Surface proteins and antigens of adult Schistosoma mansoni tegumental membranes detached onto poly-lysine coated beads.
Poly-lysine coated beads attached readily onto Schistosoma mansoni. On detachment, the beads removed membranes from the surface of the tegument. Analysis of the proteins of the detached membranes showed that three major proteins of 94, 73 and 62 kDa were present in contrast to a more complex range of proteins present in the phosphate-buffered saline released membranes. The membranes attached to beads were radio-iodinated and the antigens examined in immunoprecipitates by sodium dodecyl sulphate-polyacrylamide gel electrophoresis using various antisera. In addition to the well-established 32 and 20 kDa antigens of the tegument, other major antigens of 200, 25 and 11-12 kDa were iodinated in the membranes attached to the beads. The results suggest that the major antigens studied in the tegument may not correspond to the major proteins identified. The present approach shows promise for deducing the topography of the surface antigens and proteins of schistosomes.
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D007457:Iodine Radioisotopes; D008566:Membranes / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008863:Microspheres; D011107:Polylysine; D011506:Proteins / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000276:immunology / Q000378:metabolism*
D000947:Antigens, Helminth; D000954:Antigens, Surface; D007457:Iodine Radioisotopes; D011506:Proteins; D011107:Polylysine