The effect of low molecular weight heparin on experimental thrombosis and haemostasis--the influence of production method.
Three low molecular weight heparins prepared by enzymatic depolymerization, chemical degradation, and fractionation, respectively were studied in experimental thrombosis and haemostasis models in vivo and in biological assays in vitro. The three low molecular weight heparins, which had comparable molecular weight distributions, showed very similar activities both in vitro and in vivo. All three showed dose dependent thromboprophylactic effect. The antithrombotic effects of the low molecular weight heparins and conventional heparin administered in the same dose (30 XaI u/kg b.w.) did not differ. Neither LMW heparin nor conventional heparin (60 or 90 XaI u/kg b.w.) showed significant effects on the haemostatic plug formation time in the rabbit mesenteric microcirculation. These experiments confirm that low molecular weight heparins are potential antithrombotic drugs, which by intravenous administration have effects similar to those of standard heparin. The method of preparation seems to be of no or minor importance, at least if the molecular weight distributions of the products are similar.
D000818:Animals; D001760:Bleeding Time; D004305:Dose-Response Relationship, Drug; D004798:Enzymes / Q000494:pharmacology; D005260:Female; D006487:Hemostasis / Q000187:drug effects*; D006493:Heparin / Q000302:isolation & purification / Q000494:pharmacology*; D007275:Injections, Intravenous; D008297:Male; D008641:Mesenteric Vascular Occlusion / Q000188:drug therapy; D008970:Molecular Weight; D011817:Rabbits; D012062:Relative Biological Effectiveness; D013927:Thrombosis / Q000188:drug therapy / Q000517:prevention & control*
D004798:Enzymes; D006493:Heparin