The detection of DNA repair in bromodeoxyuridine versus thymidine containing DNA of individual cells.
This study investigates the phenomenon of unscheduled DNA synthesis (UDS) in cells whose DNA contains Bromodeoxyuridine (BrdU) using a double label methodology. The technique utilized is capable of detecting two probes simultaneously from the DNA of individual cells. A significant increase in the degree of UDS in BrdU containing cells was noted compared to cells whose DNA contained only thymidine. However, after adjusting our experimental protocol to eliminate the artifacts produced by the incomplete suppression of DNA synthesis by HU, no significant difference in the degree of UDS could be detected between the two cell populations.
D000818:Animals; D001973:Bromodeoxyuridine; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000378:metabolism; D006918:Hydroxyurea / Q000494:pharmacology; D007399:Interphase; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D013936:Thymidine
D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine; D013936:Thymidine; D006918:Hydroxyurea