Isolation of N-phenylprotoporphyrin IX from the red cells and spleen of the phenylhydrazine-treated rat.
Blood and spleens of phenylhydrazine-injected rats were treated with a solution of acidic methanol and zinc ion to isolate a green pigment. The pigment was resolved into two, I and II, by thin-layer chromatography. Pigment I was a mixture of two isomers of zinc complex of esterified N-phenylprotoporphyrin IX, in which vinyl-substituted pyrrole rings A and B were phenylated; and pigment II was a mixture of two isomers of the porphyrin complex with the N-phenyl group on propionic acid-substituted rings C and D. These pigments were also chemically prepared from the reaction of phenylhydrazine with oxyhemoglobin, independently characterized, and used to confirm the structures of the biological pigments. Determination revealed that the total amount of pigments found in the blood and spleen at 24 h after injection of phenylhydrazine corresponds to about 0.4% of the injected phenylhydrazine.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D010659:Phenylhydrazines / Q000494:pharmacology*; D011166:Porphyrins / Q000302:isolation & purification*; D011524:Protoporphyrins / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism
D010659:Phenylhydrazines; D011166:Porphyrins; D011524:Protoporphyrins; C030299:phenylhydrazine; C032609:N-phenylprotoporphyrin IX