Production of tetrodotoxin and its derivatives by Pseudomonas sp. isolated from the skin of a pufferfish.
Bacteria isolated from the skin of the pufferfish Fugu poecilonotus were screened for tetrodotoxin production. Tetrodotoxin and its derivatives were detected by HPLC analyses in broth cultures of a Pseudomonas sp. Upon alkali treatment the compounds yielded, as do tetrodotoxin and its derivatives, 2-amino-6-hydroxymethyl-8-hydroxyquinazoline, the identity of which was confirmed, after methylation or trimethylsilylation, by HPLC, mass spectrometry and gas chromatography - mass spectrometry.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005400:Fishes, Poisonous / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D012867:Skin / Q000382:microbiology; D013779:Tetrodotoxin / Q000096:biosynthesis* / Q000302:isolation & purification
D013779:Tetrodotoxin