title: Eukaryotic initiation factor 4A stimulates translation in microinjected Xenopus oocytes.
abstract: The injection of heterologous mRNA into fully grown Xenopus oocytes results not only in the synthesis of the heterologous protein but also in a reciprocal decrease in the synthesis of endogenous proteins. This indicates that injected and endogenous mRNAs compete for some component which is rate-limiting for translation in oocytes. We have attempted to identify this rate-limiting translational component. We find that heterologous and homologous polysomes compete with endogenous mRNAs as effectively as naked mRNA, indicating that polysomes do not contain detectable levels of the rate-limiting factor. In addition, we have used micrococcal nuclease digestion and a mRNA-specific oligonucleotide to destroy the mRNA component of polysomes. The remaining polysome factors, when injected into oocytes, failed to stimulate translation. When several eukaryotic translation initiation factors were injected into oocytes, initiation factor 4A consistently increased general oocyte protein synthesis by about twofold. It is possible that the availability of eIF-4A in oocytes is a key factor in limiting the overall rate of protein synthesis.
mesh_terms: D000818:Animals; D039601:Eukaryotic Initiation Factor-4A; D005786:Gene Expression Regulation; D008836:Micrococcal Nuclease; D009865:Oocytes / Q000502:physiology*; D010448:Peptide Initiation Factors / Q000494:pharmacology* / Q000502:physiology; D011132:Polyribosomes / Q000502:physiology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D039601:Eukaryotic Initiation Factor-4A; D008836:Micrococcal Nuclease