title: A simple test to monitor aromatic amine clearance in occupational workers.
abstract: A simple, quick and sensitive procedure is described to monitor urinary clearance of aromatic amines in industrial workers. Estimation is based on the measurement of coloured Schiff's bases developed with 4-dimethylaminocinnamaldehyde-organic acid reagent. Extraction of amines in water saturated butanol ensured 89-97% recovery from biological samples with a coefficient of variation of less than 8%. Regression analyses of the absorbance-concentration curves with different aromatic amines showed excellent correlation coefficient and a high molar absorptivity with the lowest detection limits ranging between 0.05-0.12 microgram ml-1. The proposed reagent has additional use as a spray reagent to detect amine spots on chromatograms. Low final acidity ensures estimates of free amines without causing hydrolysis of conjugated amines.
mesh_terms: D000588:Amines / Q000032:analysis* / Q000097:blood / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D002934:Cinnamates; D004355:Drug Stability; D004784:Environmental Monitoring; D006801:Humans; D007202:Indicators and Reagents; D009784:Occupational Diseases / Q000378:metabolism*
chemical_list: D000588:Amines; D002934:Cinnamates; D007202:Indicators and Reagents; C018523:4-dimethylaminocinnamaldehyde