Separation of two isozymes of polyamine oxidase from murine L1210 leukemia cells.
Polyamine oxidase from L1210 cells has been separated into two isozymes, A and B on the basis of chromatography on hydroxyapatite. The two isozymes also show different elution characteristics from MGBG Sepharose and Sephacryl-300. The molecular weights of A and B isozymes are estimated to be 260,000 and 200,000 daltons, respectively. The two isozymes show different kinetic characteristics with spermidine, spermine, N1-acetylspermine, and N1,N12-diacetylspermine.
D000818:Animals; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D007939:Leukemia L1210 / Q000201:enzymology*; D051379:Mice; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000302:isolation & purification* / Q000378:metabolism; D013096:Spermine / Q000031:analogs & derivatives; D013379:Substrate Specificity; D000097667:Polyamine Oxidase
D007527:Isoenzymes; D000587:Oxidoreductases Acting on CH-NH Group Donors; D013096:Spermine; D000097667:Polyamine Oxidase; C029452:N'-acetylspermine; C029453:N',N''-diacetylspermine