Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro.
P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells have an absolute requirement for cholesterol for growth under serum-free conditions. This requirement can be satisfied by low density lipoprotein at 2-6 micrograms/ml or by BSA-bound cholesterol at 5-10 micrograms/ml. Cholesterol-independent variants can be selected after prolonged growth in low concentrations of serum.
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology; D051379:Mice; D009101:Multiple Myeloma / Q000473:pathology*
D003470:Culture Media; D008077:Lipoproteins, LDL; D002784:Cholesterol