[Kinetic characteristics of 3,4-benzpyrene hydroxylation in the liver microsomes of mice].
The kinetic parameters of binding and hydroxylation of hydrophobic substrate 3,4-benzpyrene have been studied in liver microsomes of untreated and 3-methylcholanthrene treated mice. The reaction of benzpyrene-hydroxylase has been established to be described by hyperbolic curve, which characterizes the dependence of [ES] and d(P)/dt on [E0] for reactions in biphasic system. A key role of microsomal membraneous phospholipids has been revealed in competitive inhibition of 3,4-benzpyrene hydroxylation. For the adequate application of Michaelis--Menten theory for benzpyrene-hydroxylation reaction a modified method of 3,4-benzpyrene-hydroxylation in the samples with low content of protein in microsomal fraction is suggested.
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D006900:Hydroxylation; D007700:Kinetics; D008563:Membrane Lipids / Q000378:metabolism; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D008563:Membrane Lipids; D010743:Phospholipids; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene
Kineticheskie kharakteristiki gidroksilirovaniia 3,4-benzpirena v mikrosomakh pecheni mysheĭ.