Fluorometric documentation of increased cutaneous blood flow after topical application of a PGE2 analog in man.
The present study employed fiberoptic fluorometry, a noninvasive means of documenting delivery and removal of fluorescein dye, to evaluate the local circulatory changes elicited by topical application of DHV-PGE2 ME, an investigational PGE2 analog. On Day 1, inactive vehicle was applied to a 5 X 4 cm study site on each thigh of healthy volunteer subjects (n = 12). Symmetrical perfusion was confirmed by similar determinations of dye delivery and removal at each site. On Day 2, DHV-PGE2 ME, 30 or 120 micrograms, was applied to one site while inactive vehicle again was applied to the other. After administration of 120 micrograms in a petrolatum vehicle, fluorometry detected a pronounced increase in nutritive perfusion. There was significant acceleration of dye delivery and removal (p less than 0.05 by ANOVA). Less pronounced changes were noted after the lower dose of DHV-PGE2 ME and when the drug was applied in a triethyl citrate vehicle. The local circulatory changes were not accompanied by systemic effects; there were no changes in vital signs or in fluorometric indices at remote sites.
D000293:Adolescent; D000328:Adult; D015232:Dinoprostone / Q000031:analogs & derivatives*; D005336:Fiber Optic Technology; D005470:Fluorometry; D006801:Humans; D007700:Kinetics; D008297:Male; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply*
D011459:Prostaglandins E, Synthetic; C034231:15-deoxy-16-hydroxy-16-vinylprostaglandin E2; D015232:Dinoprostone