Identification of two forms of molybdate-stabilized, non-transformed glucocorticoid hormone-receptor complex by gel filtration chromatography.
Glucocorticoid-receptor complexes in rat thymus cytosol were characterized by gel-filtration chromatography on Agarose A-1.5 m and Sephacryl S-300. Two forms of non-transformed complex were identified at low ionic strength in the presence of molybdate, with Stokes radii of approx 8 nm and 6 nm. The 8 nm molybdate-stabilized form could be converted to the 6 nm form by chromatography on Sephacryl S-300 or Lipidex 1000 or by incubation with dextran-charcoal or phospholipase C, but not by chromatography on Sephadex G-25; none of the treatments promoted receptor transformation. It is suggested that the change in Stokes radius from 8 to 6 nm results from the removal of a lipid factor responsible for maintaining the complex in the 8 nm form.
D000818:Animals; D002850:Chromatography, Gel; D005938:Glucocorticoids / Q000032:analysis*; D008297:Male; D008982:Molybdenum / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis; D010738:Type C Phospholipases / Q000494:pharmacology
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; C044659:molybdate; D008982:Molybdenum; D010738:Type C Phospholipases