7-Hydro-8-methylpteroylglutamylglutamic acid, a new anti-folate from an actinomycete. Fermentation, isolation, structure and biological activity.
A new anti-folate, 7-hydro-8-methylpteroylglutamylglutamic acid, was isolated from the culture broth of a soil actinomycete. The antibiotic inhibited the growth of Enterococcus faecium which requires folic acid. The inhibitory action was reversed by thymidine or excess amounts of folate-related compounds such as pteroic acid, folic acid, dihydrofolic acid and leucovorin. It inhibited thymidylate synthase from E. faecium, Bacillus subtilis and Ehrlich ascites carcinoma cells.
D000192:Actinomycetales / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D005285:Fermentation; D005492:Folic Acid / Q000031:analogs & derivatives*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D011624:Pteroylpolyglutamic Acids / Q000302:isolation & purification* / Q000627:therapeutic use; D013056:Spectrophotometry, Ultraviolet; D013940:Thymidylate Synthase / Q000037:antagonists & inhibitors
D000900:Anti-Bacterial Agents; D011624:Pteroylpolyglutamic Acids; C052093:7-hydro-8-methylpteroylglutamylglutamic acid; D005492:Folic Acid; D013940:Thymidylate Synthase