Application of a simple methylation procedure for the analyses of glycosphingolipids.
Acid and neutral glycosphingolipids (0.01-1 mumol) were completely methylated in high yields and with little formation of by-products in 10 min at room temperature, using methyl sulphoxide, methyl iodide, and powdered NaOH. Re-methylation of methylated and LiAlH4-reduced gangliosides gave a new derivative that was useful for the analysis of gangliosides by mass spectrometry.
D000818:Animals; D002554:Cerebrosides; D002849:Chromatography, Gas; D005732:Gangliosides / Q000302:isolation & purification; D006028:Glycosphingolipids / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008745:Methylation
D002554:Cerebrosides; D005732:Gangliosides; D006028:Glycosphingolipids; D007202:Indicators and Reagents