Modification of the radiation induced damage by 2-deoxy-D-glucose in organ cultures of human cerebral gliomas.
Effects of a glucose antimetabolite, 2-deoxy-D-glucose (2-DG), on the gamma ray induced radiation damage have been studied in organ cultures of human cerebral gliomas. Percentage of cells with micronuclei (M-fraction) was used to assay the radiation damage. Experimental data indicate the following results. Untreated cerebral gliomas show considerable spatial heterogeneity in M-fraction; In spite of this heterogeneity, increases in M-fraction induced by gamma rays can be clearly observed, if multiple and randomly selected explants are analyzed for each group; The radiation induced M-fraction in different gliomas varies over a wide range; Presence of 2-DG (5 mM) for 4 h after irradiation leads to an increase in the radiation induced M-fraction in the majority of tumors, while in a smaller number (congruent to 25%) a decrease is observed under similar conditions. These results can be explained on the basis of a model postulating differential effects of 2-DG on the energy linked modulations of the processes of repair and fixation of DNA damage, which competitively influence the formation of micronuclei.
D001932:Brain Neoplasms / Q000532:radiotherapy* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000528:radiation effects; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D005720:Gamma Rays; D005910:Glioma / Q000532:radiotherapy* / Q000648:ultrastructure; D006801:Humans; D008954:Models, Biological; D009924:Organ Culture Techniques
D004273:DNA, Neoplasm; D003837:Deoxy Sugars; D003847:Deoxyglucose