Expression and properties of two types of protein kinase C: alternative splicing from a single gene.
Two complementary DNA's, encoding the complete sequences of 671 and 673 amino acids for subspecies of rat brain protein kinase C, were expressed in COS 7 cells. The complementary DNA sequence analysis predicted that the two enzymes are derived from different ways of splicing and differ from each other only in the short ranges of their carboxyl-terminal regions. Both enzymes showed typical characteristics of protein kinase C that responded to Ca2+, phospholipid, and diacylglycerol. The enzymes showed practically identical physical and kinetic properties and were indistinguishable from one of the several subspecies of protein kinase C that occurs in rat brain but not in untransfected COS 7 cells. Partial analysis of the genomic structure confirmed that these two subspecies of protein kinase C resulted indeed from alternative splicing of a single gene.
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000235:genetics; D009693:Nucleic Acid Hybridization; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D011817:Rabbits; D051381:Rats
D004247:DNA; D011493:Protein Kinase C