title: The binding of glucosylceramidase to glucosylceramide is promoted by its activator protein.
abstract: A protein activator of glucosylceramidase (EC 3.2.1.45) has been previously identified by us in human placenta [(1985) Biochim. Biophys. Acta 836, 157-166]. In the present paper we report that its function in vitro is to stimulate the binding of the enzyme to its substrate, glucosylceramide. After the purification step which frees the enzyme of most of its activator protein (octyl-Sepharose 4B chromatography), the capacity of glucosylceramidase to bind to the glucosylceramide micelles is dramatically decreased. The addition of the activator protein to the purified enzyme restores this binding.
mesh_terms: D002499:Centrifugation, Density Gradient; D002554:Cerebrosides / Q000378:metabolism*; D004789:Enzyme Activation; D005959:Glucosidases / Q000378:metabolism*; D005962:Glucosylceramidase / Q000378:metabolism*; D005963:Glucosylceramides / Q000378:metabolism*; D006023:Glycoproteins; D006801:Humans; D008823:Micelles; D010920:Placenta / Q000201:enzymology*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D049231:Saposins; D049128:Sphingolipid Activator Proteins
chemical_list: D002554:Cerebrosides; D005963:Glucosylceramides; D006023:Glycoproteins; D008823:Micelles; C483345:PSAP protein, human; D011506:Proteins; D049231:Saposins; D049128:Sphingolipid Activator Proteins; D005959:Glucosidases; D005962:Glucosylceramidase