A simplified method for freezing and storage of human semen.
The use of 10-cm-long plastic tuberculin syringes for freezing and storage of human semen is introduced. This new method reduces the number of prefreezing and postthawing manipulations to a minimum. Postthawing motility, penetration of spermatozoa into cervical mucus, and results of the hamster ovum test were similar for spermatozoa stored-frozen in tuberculin syringes and for spermatozoa stored-frozen in french straws. The results of the postinsemination test performed in six women were slightly better with fresh semen than with semen stored-frozen in tuberculin syringes. Donor insemination with fresh semen resulted in eight pregnancies per 24 insemination cycles; with semen stored-frozen in tuberculin syringes five pregnancies were achieved in 22 insemination cycles.
D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000295:instrumentation / Q000379:methods*; D013048:Specimen Handling; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013594:Syringes