title: [Gel chromatography (GPC) as a clean-up procedure in residue analysis of veterinary drugs].
abstract: In this study we investigated the chromatographic behaviour of 40 veterinary drugs belonging to different classes (sulphonamides, nitrofurans, nitroimidazoles, antibiotics, anthelmintics, psychopharmacological substances, growth promoters) using the gel permeation media Sephadex LH-20, Bio Beads S-X 3 and Fractogel PGM 2,000. To allow the use of acetic acid in mobile phases all parts containing brass in the GPC equipment (Autoprep 1002 A) had to be exchanged for stainless steel components. The curves of elution with Sephadex LH-20 as stationary phase demonstrated good separation of the majority of drugs from co-extractives from liver, kidney, muscle, egg and milk by gravimetric measurement as well as by UV absorption. A clean-up of similar efficiency could however also be obtained with Sep-Pak silica cartridges, if final residue analysis was made by HPLC with UV detection.
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000032:analysis; D008892:Milk / Q000032:analysis; D009132:Muscles / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D014730:Veterinary Medicine
chemical_list: D004364:Pharmaceutical Preparations
vernacular_title: Untersuchungen zur Gelchromatographie (GPC) als Reinigungsverfahren in der Rückstandsanalytik von Tierarzneimitteln.