Subcellular distribution of glucocorticoid receptor in the neoplastic epithelial duct cell line from human salivary gland.
Neoplastic epithelial duct cell line from human salivary gland (HSG cell line) contains the specific glucocorticoid receptor. The time course study on the uptake of [3H]triamcinolone acetonide (TA), a synthetic glucocorticoid, by intact HSG cells in a growing monolayer culture showed that translocation of glucocorticoid receptors into nuclei occurred at 37 degrees C, but not at 0 degrees C. To elucidate the subcellular distribution of glucocorticoid receptor from HSG cells, a scaled-up-culture was employed. When the cells were incubated with [3H]TA at 0 degrees C, 94% of the receptors were found in the cytosol fraction, while only 6% of the receptors existed in the nuclei. When the cells were incubated at 37 degrees C, 49% of the receptor complexes were distributed in the nuclei and 74% of these nuclear receptor complexes were extractable with 5 mM pyridoxal phosphate.
D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D003600:Cytosol / Q000032:analysis; D006801:Humans; D011189:Potassium Chloride / Q000494:pharmacology; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000032:analysis*; D014222:Triamcinolone Acetonide / Q000378:metabolism
D011965:Receptors, Glucocorticoid; D011189:Potassium Chloride; D014222:Triamcinolone Acetonide