Quantitative imaging and microanalysis with a scanning soft x-ray microscope.
A scanning soft x-ray microscope has been developed that uses synchrotron radiation focused by a Fresnel zone plate to form a submicron beamspot on the specimen. Transmitted x-rays are detected and used to form a quantitative map of specimen absorptivity. Applications of the instrument to the imaging of whole wet cells and to the mapping of calcium in sections of bone are presented, with a resolution of 300 nm and an elemental sensitivity of 2 micrograms cm-2.
D000818:Animals; D001369:Axons / Q000000981:diagnostic imaging / Q000648:ultrastructure; D002478:Cells, Cultured; D004577:Electron Probe Microanalysis / Q000379:methods; D006801:Humans; D009474:Neurons / Q000166:cytology / Q000000981:diagnostic imaging*; D011859:Radiography; D012886:Skull / Q000000981:diagnostic imaging*; D014965:X-Rays