Primary structure of the Dolichos biflorus seed lectin.
The Dolichos biflorus seed lectin is a tetramer composed of equal amounts of two subunit types. The subunit types are structurally very similar, yet only the larger subunit exhibits the ability to bind carbohydrate. A cDNA clone representing the entire coding region of the D. biflorus lectin mRNA has been sequenced. This cDNA represents 1075 nucleotides of seed lectin mRNA encoding a polypeptide of Mr = 29,674. Analysis of the deduced sequence indicates that the NH2 termini and COOH termini of both lectin subunits are present within the mRNA coding region. This information supports previous data indicating that both subunits of the lectin are encoded by a single mRNA and that the difference between the subunit types apparently arises by the proteolytic removal of a 10-amino acid sequence from the COOH terminus of the larger subunit. Comparison of the D. biflorus seed lectin sequence to the sequence of other leguminous seed lectins indicates regions of extensive homology. The residues of concanavalin A involved in metal binding are highly conserved in the D. biflorus lectin, but those involved in saccharide binding show a much lower degree of conservation. Prediction of the secondary conformation of the D. biflorus polypeptide suggests that structures involved in the formation of quaternary structure in concanavalin A are also conserved.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D003208:Concanavalin A; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D007887:Fabaceae; D037102:Lectins / Q000235:genetics; D008670:Metals / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics; D037121:Plant Lectins; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000235:genetics
D004274:DNA, Recombinant; D037102:Lectins; D008670:Metals; D010446:Peptide Fragments; D037121:Plant Lectins; D012333:RNA, Messenger; C036554:dolichos biflorus agglutinin; D003208:Concanavalin A; D004247:DNA