A simple fast micromethod for measuring enzyme activities which release tritium as tritium water.
The entire procedure is carried out in a counting vial by mixing the reagents as a 20- to 30-microliters drop in the cap of a counting vial, incubating, quenching the reaction, and then distilling the tritium water produced into the chilled vial, in which it is assayed after the addition of scintillation solvent and a clean cap. The application of this technique to the analysis of serum transaminases is described.
D006801:Humans; D000637:Transaminases / Q000097:blood*; D014316:Tritium; D014867:Water
D014867:Water; D014316:Tritium; D000637:Transaminases