The capacity of macrophages from different murine tissues to metabolise ethanol and generate an ethanol-dependent non-dialysable cytotoxic activity in vitro.
Tissue macrophages obtained from liver, bone marrow, spleen and thymus of C57 BL/6 mice closely resembled blood-monocyte-derived human macrophages in three characteristics. These were: the rate of metabolism of ethanol to acetate, the biochemical pathways involved in ethanol metabolism and the ability to generate an ethanol-dependent non-dialysable cytotoxic activity in vitro. The metabolism of ethanol by all four types of murine tissue macrophage was only slightly suppressed by pyrazole, 4-iodopyrazole and 3-amino-1,2,4-triazole, which are known to inhibit alcohol dehydrogenase (ADH), pi ADH and catalase respectively. By contrast, ethanol metabolism by these cells was strongly suppressed by three inhibitors of the cytochrome P-450-dependent microsomal ethanol-oxidising system--namely, carbon monoxide, metyrapone and tetrahydrofurane.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D000431:Ethanol / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
D000085:Acetates; D000431:Ethanol