Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells.
A cDNA library was constructed from the mRNA of the ZR-75 oestrogen responsive human breast cancer cell line and screened for oestrogen regulated mRNA sequences. Of the recombinants isolated, 30 contained cDNA that corresponded to a single mRNA species of 2.1 kb that was induced between 10 and 15 fold by oestradiol treatment. The sequence of the entire open reading frame and 3' non-coding region of the mRNA was determined and shown to encode the aspartyl protease cathepsin D. The induction of cathepsin D mRNA is specific for oestrogen and is maximal at 3 X 10(-10) M. Cathepsin D mRNA levels were increased by oestrogen in 3 oestrogen responsive breast cancer cell lines. Cathepsin D mRNA was expressed but not regulated in an oestrogen receptor negative breast tumour cell line, BT 20, and in 2 other malignant cell lines, Hela and A431.
D001483:Base Sequence; D001943:Breast Neoplasms / Q000032:analysis* / Q000473:pathology; D002402:Cathepsin D / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004790:Enzyme Induction / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009376:Neoplasms, Hormone-Dependent / Q000032:analysis* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000302:isolation & purification
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D004958:Estradiol; D004247:DNA; D002402:Cathepsin D