title: [Method of simultaneous analysis of vitamins A, E and beta-carotene in dairy products].
abstract: A method has been suggested for simultaneous assay of vitamins A, E and beta-carotene in milk products. A specimen sample is subjected to alkaline hydrolysis, then vitamins are extracted and separated in the column with aluminium oxide; for beta-carotene elution hexane is used, for vitamin E - acetone-hexane (10:90), for vitamin A - acetone-hexane (15:85). beta-carotene is assayed in hexane by absorption. Vitamin A and E fractions are evaporated, the residue is diluted in absolute alcohol, vitamin A is identified by absorption, vitamin E - by the colour Emmery-Engel reaction. The variation coefficient in the assay of vitamins A, E and beta-carotene was less than 10% in 5 out of 6 cases.
mesh_terms: D002338:Carotenoids; D002845:Chromatography; D003611:Dairy Products / Q000032:analysis*; D008722:Methods; D013053:Spectrophotometry; D014801:Vitamin A / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*; D019207:beta Carotene
chemical_list: D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids
vernacular_title: Metod odnovremennogo opredeleniia vitaminov A, E i beta-karotina v molochnykh produktakh.