title: Batch to batch imprecision in the affinity chromatography assay of glycated hemoglobin.
abstract: An affinity chromatographic method (AC) was compared with ion exchange chromatography (IEC) for determination of glycated hemoglobin (gHb). In vitro, increasing glucose concentration produced no change on AC-determined gHb. gHb measurement by AC was highly correlated with mean blood glucose (r = 0.96). Mean values of gHb were apparently higher, and variation greater, by AC than by IEC. Intra-assay variation was 2.0% and 3.0%, respectively. Inter-assay variation was 4.8% by IEC and 6.4% by AC. When different batches of AC-columns were compared, mean values as well as precision differed considerably. The affinity chromatographic method tested here shows several expected advantages, but precision and inter-batch variation of the columns remain to be improved.
mesh_terms: D001786:Blood Glucose / Q000032:analysis; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin