Fast transmembrane exchange in red cells studied with NMR.
13C NMR, with two fundamentally different procedures, was used to measure the H13CO3- permeability coefficient (P) of human erythrocytes. The values of P (approximately 3 X 10(-4) cm s-1) were similar to those obtained by other, non-NMR methods. A third procedure was used with 31P NMR to measure the permeability coefficient of human erythrocytes for the non-electrolyte dimethyl methylphosphonate; the effects on transmembrane exchange of varying haematocrit and butanol were studied.
D001639:Bicarbonates / Q000097:blood; D001693:Biological Transport, Active; D002463:Cell Membrane Permeability; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009943:Organophosphorus Compounds / Q000097:blood
D001639:Bicarbonates; D009943:Organophosphorus Compounds; C031116:dimethyl methylphosphonate