A new ribonuclease from cobra venom (Naja naja) showing specificity towards cytidylic acid.
A ribonuclease was isolated and purified to homogeneity from cobra venom. The enzyme was found to be homogeneous on SDS and non-SDS polyacrylamide gel electrophoreses, and high performance liquid chromatography. The purified enzyme hydrolysed poly(rC), but not poly(rA), poly(rU), poly(rG), poly(rI), or poly(rI).poly(rC). The presence of magnesium was found to be an essential requirement for the nucleolytic activity of this enzyme.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003568:Cytidine Monophosphate / Q000378:metabolism*; D003597:Cytosine Nucleotides / Q000378:metabolism*; D004546:Elapid Venoms / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008274:Magnesium / Q000494:pharmacology; D011061:Poly A / Q000378:metabolism; D011066:Poly C / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity
D003597:Cytosine Nucleotides; D004546:Elapid Venoms; D011061:Poly A; D011072:Poly U; D011066:Poly C; D012260:Ribonucleases; D003568:Cytidine Monophosphate; D008274:Magnesium