Choosing near-linear parameters in the four-parameter logistic model for radioligand and related assays.
Ten parameters extracted from six currently used parametrizations of the four-parameter logistic model, and one new proposal, were examined for their statistical behavior in nonlinear least-squares estimation in combination with ELISA and RIA data. Those which are adequately near-linear on the basis of the Lowry-Morton lambda statistic were identified and can be recommended for use in practice.
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007986:Luteinizing Hormone / Q000032:analysis; D008433:Mathematics; D008962:Models, Theoretical; D011863:Radioimmunoassay / Q000379:methods; D011869:Radioligand Assay / Q000379:methods*; D014805:Vitamin B 12 / Q000032:analysis
D007073:Immunoglobulin E; D007986:Luteinizing Hormone; D014805:Vitamin B 12