Determination of putrescine in brain tissue using gas chromatography-mass spectrometry.
We used gas chromatography-mass spectrometry to assay putrescine in minute regions of single rat brains. Acid extraction, partial purification on Amberlite CG 120, and derivatization with pentafluoropropionic anhydride preceded the gas chromatography-mass spectrometry. A moving-needle solventless system and a direct inlet system were also used to increase sensitivity. Putrescine was measured accurately at the picomole level; the mean concentration of this polyamine in five regions of rat brain found by this method was 2.7-3.8 times higher than reported by other researchers.
D000818:Animals; D001923:Brain Chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D008832:Microchemistry; D011700:Putrescine / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D011700:Putrescine