High-performance liquid chromatographic separation and partial characterization of human protamines 1, 2, and 3.
A method for separating the three human protamines by HPLC of underivatized, total protamine extracts on a Nucleosil RP-C18 column is described. The identities of the three proteins have been confirmed by a combination of disc gel electrophoresis, amino acid composition, and primary sequence analysis. The results show that human protamine 3 elutes first, closely followed by protamine 2. Protamine 1 elutes later. The amino acid compositions and partial amino terminal sequences of human protamines 2 and 3 indicate that these two proteins are very closely related and suggest that they differ only by three amino-terminal amino acids.
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D011479:Protamines / Q000032:analysis / Q000302:isolation & purification*; D012661:Semen / Q000032:analysis*
D000596:Amino Acids; D011479:Protamines; C046533:protamine P1; C048529:protamine P2; C052196:protamine P3