Hydrophobic labelling of spectrin in erythrocytes using arylisothiocyanates.
Labelling of spectrin with the hydrophobic probes 2-naphthylisothiocyanate and 4-[125I]-iodophenylisothiocyanate dispersed in lipid vesicles was studied. Although spectrin is a peripheral protein of the erythrocyte membrane, it was found to be labelled as a result of incubation of washed red blood cells with hydrophobic arylisothiocyanates.
D015058:1-Naphthylisothiocyanate; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D017879:Isothiocyanates; D013049:Spectrin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013861:Thiocyanates
D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D017879:Isothiocyanates; D013861:Thiocyanates; D013049:Spectrin; C052479:4-iodophenylisothiocyanate; D015058:1-Naphthylisothiocyanate; C031287:2-naphthylisothiocyanate