Activities of NAD-and NADP-specific isocitrate dehydrogenases in kidney mitochondria of rachitic rat.
In the previous research we have demonstrated that rats fed on a rickets-inducing diet show increasing citrate levels in kidney and intestinal mucosa. The study of the enzymes related to citrate metabolism has shown that both NAD+-and NADP+-dependent isocitrate dehydrogenases decrease in kidney mitochondria of rachitic rat. The inhibitory effect of Ca2+ and citrate on the activity of the two dehydrogenases has been also investigated; these metabolites behave as competitive inhibitors against Mg2+ both in normal and in rachitic rats.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D012279:Rickets / Q000378:metabolism*
D002951:Citrates; D010710:Phosphates; D009243:NAD; D009249:NADP; D007521:Isocitrate Dehydrogenase; D000469:Alkaline Phosphatase; D008274:Magnesium; D002118:Calcium