The synthesis of glucose and ammonia by kidney tubules isolated from suckling and early-weaned lambs.
Glucose and ammonia production were examined in kidney tubules isolated from suckling and early-weaned lambs, on days 10-30 after birth, with abrupt weaning occurring at day 14. There were no differences in the rates of glucose or ammonia production for a given substrate by tubules isolated from any of the lambs, regardless of age or stage of weaning. The preferred substrates for gluconeogenesis were glycerol = lactate greater than propionate = pyruvate = fructose = proline greater than alanine greater than glutamate greater than glutamine greater than aspartate greater than glycine greater than serine, and for ammoniagenesis were glutamine much greater than alanine greater than aspartate much greater than serine greater than glycine = glutamate = proline.
D000375:Aging; D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D050260:Carbohydrate Metabolism; D005947:Glucose / Q000096:biosynthesis*; D007684:Kidney Tubules / Q000254:growth & development* / Q000378:metabolism*; D012756:Sheep; D014886:Weaning
D000596:Amino Acids; D000641:Ammonia; D005947:Glucose