Erythrocyte metabolism in hyperthyroidism: a microcalorimetric study on changes in the Embden-Meyerhof and the hexose monophosphate pathways.
Erythrocyte metabolism was studied in vitro by microcalorimetry in 10 hyperthyroid subjects before and after treatment. By inhibiting the enzyme enolase in the Embden-Meyerhof pathway with sodium fluoride (NaF) we have recorded the anaerobic and aerobic contributions in erythrocyte thermogenesis. The decrease in heat production rate in samples with NaF corresponds to the anaerobic contribution, whereas the values from samples with NaF reflect aerobic processes. Before treatment, total heat production rate was 120 +/- 2 mW/l erythrocytes which was higher than the post-treatment value of 99 +/- 2 (P less than 0.001) as well as the value for 14 euthyroid subjects, 108 +/- 2 mW/l (P less than 0.001). The NaF inhibitable rate was 73 +/- 2 before and 63 +/- 1 mW/l after therapy (P less than 0.01). These values correspond to 61 +/- 1 and 64 +/- 1% (n.s.) of the total heat production rate, and were similar to that of 61 +/- 2% for the controls. Heat production rates in the presence of NaF were 47 +/- 1 before and 36 +/- 1 mW/l after therapy (P less than 0.001), representing 39 +/- 1 and 36 +/- 1% of total values, respectively. The present results show that overall metabolism is increased in erythrocytes from hyperthyroid subjects before treatment and returns to normal after normalization of the thyroid function. Moreover, by using microcalorimetry we found that the metabolic activity along the Embden-Meyerhof anaerobic pathway as well as along the hexose monophosphate aerobic pathway in erythrocytes is stimulated by thyroid hormones.
D000328:Adult; D000368:Aged; D002151:Calorimetry; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006019:Glycolysis / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010427:Pentose Phosphate Pathway; D010751:Phosphopyruvate Hydratase / Q000097:blood; D012969:Sodium Fluoride / Q000494:pharmacology; D013963:Thyroid Hormones / Q000097:blood
D013963:Thyroid Hormones; D012969:Sodium Fluoride; D010751:Phosphopyruvate Hydratase