title: Recovery of cholinesterases in soman-injected superior cervical ganglion of the rat in the presence and absence of innervation.
abstract: We have previously described the procedure for quantitative separation of extracellular and intracellular ChEs using mild treatment of rat superior cervical ganglion with papain. Here, this procedure was used in order to investigate the recovery of ChEs in the two pools after irreversible inhibition by soman which was directly injected into the ganglion. After such treatment only ganglion ChEs were totally inhibited, whereas the activity of ChEs in preganglionic neurons and their axons remained unaffected. Comparing in innervated and decentralized ganglia the pattern of recovery rate and ultrastructural reappearance of ChEs after local inhibition, with that reported after systemic ChEs inhibition, it was possible to distinguish between the indirect effects of innervation on the recovery rate and pattern of ChEs of ganglion origin and the direct contribution to the total ganglion enzyme activity of ChEs originating in the preganglionic elements. The absence of nerve contracts affects mostly extracellular activity, particularly AChE, whereas the intracellular activity of AChE was only slightly decreased and the activity of nsChE was somewhat increased. This increase coincides with the enhanced cytochemical reaction of nsChE in some nonneuronal cells in the ganglion. Actinomycyn D decreased the rate of initial rapid phase of recovery of intracellular ChEs when injected in the ganglion daily for three days, whereas the recovery of extracellular ChEs was already decreased the first day of Actinomycyn D application. This indicates that the externalization of the enzyme is more affected than its synthesis by inhibition the translation step.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D002802:Cholinesterases / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D003714:Denervation; D005728:Ganglia, Sympathetic / Q000201:enzymology* / Q000648:ultrastructure; D006651:Histocytochemistry; D007267:Injections; D008854:Microscopy, Electron; D051381:Rats; D012999:Soman / Q000494:pharmacology*
chemical_list: D003609:Dactinomycin; D012999:Soman; D002802:Cholinesterases