title: Abnormally high values for direct bilirubin in the serum of newborns as measured with the DuPont aca.
abstract: To assess notably high measurements of direct bilirubin in the serum of newborns obtained on the DuPont aca analyzer, the authors compared results from the aca instrument with the results from the Technicon RA-1000 analyzer. Total and direct bilirubin values in 11 serum samples of newborn babies were measured in parallel on both instruments. There was good correspondence between the two instruments in determination of total bilirubins. However, the diazotized sulfanilic acid method (used by both instruments) yielded much higher direct bilirubin values on the aca analyzer than on the RA-1000. The possibly spuriously elevated direct bilirubin results produced by the aca may cause clinical confusion.
mesh_terms: D001663:Bilirubin / Q000097:blood*; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D003979:Diazonium Compounds; D006801:Humans; D007231:Infant, Newborn; D009994:Osmolar Concentration; D012977:Sodium Nitrite; D013425:Sulfanilic Acids
chemical_list: D003979:Diazonium Compounds; D013425:Sulfanilic Acids; D012977:Sodium Nitrite; D001663:Bilirubin; C000469:diazobenzenesulfonic acid