Bubble free cell culture aeration with porous moving membranes.
For the cultivation of mammalian cells a new aeration system was developed. Its construction with hydrophobic porous Accurel hollow fiber membranes and the use in reactors with 1 and 20 l reaction volume will be presented and introduced by means of the cultivation of a genetically manipulated mouse cell line. The mass transfer capacity of this system is demonstrated as a function of gas flow rates through the membrane and of the membrane movement.
D000818:Animals; D001692:Biological Transport; D001709:Biotechnology / Q000295:instrumentation*; D002455:Cell Division; D002478:Cells, Cultured / Q000502:physiology*; D005374:Filtration; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D010100:Oxygen; D014867:Water
D008567:Membranes, Artificial; D014867:Water; D010100:Oxygen