High-level, heat-regulated synthesis of proteins in eukaryotic cells.
Plasmids have been constructed in which promoters of 70-kDa heat-shock protein genes (hsp70) of human and Drosophila origin were linked to three different eukaryotic genes encoding human growth hormone (hGH), chicken lysozyme (cL) and a human influenza haemagglutinin (HA). Following transfection into widely divergent eukaryotic cells, the hybrid genes direct the transient, heat-regulated synthesis of the three proteins. hGH and cL are secreted into the medium. A human hsp70-hGH construct was used to establish stable mouse fibroblast lines that are capable of producing and secreting hGH at high levels following heat induction: hGH is secreted at a 500-1200-fold higher rate by heat-treated than by untreated cells.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D013006:Growth Hormone / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006389:Hemagglutinins, Viral / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D009113:Muramidase / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014981:Xenopus
D006360:Heat-Shock Proteins; D006389:Hemagglutinins, Viral; D013006:Growth Hormone; D009113:Muramidase