Complementary base-pairing properties of cyclized and linear oligonucleotides.
Oligouridylates of varying chain lengths were synthesized by polynucleotide phosphorylase and cyclized by RNA ligase. Over chain lengths from 7 to 15, the bindings of the cyclized and linear oligomers to polyadenylate were measured on the basis of differential migration of bound and free oligomers on a gel exclusion column. Binding of the cyclized oligomers was found to be far weaker than that of their linear counterparts of equal length. Such a general reduction in base-pairing capacity due to the cyclized conformation, by limiting the strength of unintended base-pairing without obstructing the possible development of strong specific base-pairing, may represent an advantage important to the function and evolution of loop structures in tRNA and other RNA molecules.
D001482:Base Composition; D001665:Binding Sites; D009690:Nucleic Acid Conformation; D009712:Nucleotides, Cyclic / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis; D009843:Oligoribonucleotides / Q000138:chemical synthesis; D011061:Poly A; D014500:Uracil Nucleotides / Q000138:chemical synthesis
D009712:Nucleotides, Cyclic; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D014500:Uracil Nucleotides; C027916:oligo(U); D011061:Poly A