Effect of tri-iodothyronine on protein turnover in rat hepatocyte primary cultures.
The effect of tri-iodothyronine (T3) on protein turnover was studied using primary cultures of rat hepatocytes. Protein synthesis was significantly stimulated in cells cultured for 6 days in the presence of T3 (1 mumol/l). Protein secretion into the culture medium was not affected by the hormone. Breakdown of long-lived proteins, the bulk of cellular proteins which are preferentially degraded through the autophagic lysosomal pathway, was significantly stimulated by the hormone. It is concluded that T3 elicits a general stimulation of protein turnover in cultured hepatocytes.
D000818:Animals; D002401:Cathepsin B / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology*
D011506:Proteins; D014284:Triiodothyronine; D002401:Cathepsin B; D002402:Cathepsin D