title: Changes in the quaternary structure of phosphoenolpyruvate carboxylase induced by ionic strength affect its catalytic activity.
abstract: Phosphoenolpyruvate carboxylase from maize leaves dissociated into dimers and/or monomers when exposed to increasing ionic strength (e.g. 200-400 mM NaCl) as indicated by gel filtration experiments. Changes in the oligomerization state were dependent on pH, time of preincubation with salt and protein concentration. A dissociation into dimers and monomers was observed at pH 8, while at pH 7 dissociation into the dimeric form only was observed. Exposure of the enzyme to higher ionic strength decreased the activity in a time-dependent manner. Turnover conditions and glucose 6-phosphate protected the carboxylase from the decay in activity, which was faster at pH 7 than at pH 8. The results suggest that changes in activity of the enzyme, following exposure to high ionic strength, are the consequence of dissociation. Tetrameric and dimeric forms of the phosphoenolpyruvate carboxylase seemingly reveal different catalytic properties. We suggest that the distinct catalytic properties of the different oligomeric species of phosphoenolpyruvate carboxylase and changes in the equilibrium between them could be the molecular basis for an effective regulation of metabolite levels by this key enzyme of C4 plants.
mesh_terms: D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002384:Catalysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D010730:Phosphoenolpyruvate Carboxylase / Q000037:antagonists & inhibitors*; D012965:Sodium Chloride / Q000494:pharmacology; D003313:Zea mays / Q000201:enzymology
chemical_list: D005958:Glucosephosphates; D046911:Macromolecular Substances; D012965:Sodium Chloride; D019298:Glucose-6-Phosphate; D002262:Carboxy-Lyases; D010730:Phosphoenolpyruvate Carboxylase