Experimental autoimmune thyroiditis induced by a 5-10-kDa tryptic fragment from porcine thyroglobulin.
Monoclonal autoantibodies obtained using the native porcine thyroglobulin (Tg) molecule for immunization were shown to react against a defined epitope of the Tg molecule. Its biochemical characterization was undertaken, using trypsin treatment of Tg, followed by gel filtration, affinity chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high pressure liquid chromatography analysis. Immunoreactivity tested by immunoblotting with appropriate monoclonal anti-Tg antibody allowed the detection of one peptide fragment of 5-10 kDa, after limited proteolysis with trypsin. We demonstrated that this peptide reacts exclusively with monoclonal anti-Tg antibodies which block in vitro proliferative T cell response; moreover, the 5-10-kDa tryptic fragments from porcine Tg were able to induce thyroiditis when injected into normal CBA mice, while porcine Tg depleted from these fragments was not able to do so.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D006651:Histocytochemistry; D007114:Immunization; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D013552:Swine; D013954:Thyroglobulin / Q000276:immunology* / Q000378:metabolism; D013961:Thyroid Gland / Q000166:cytology / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000139:chemically induced / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D013954:Thyroglobulin; D014357:Trypsin