title: Development of pig blastocysts in a uterine environment advanced by exogenous oestrogen.
abstract: Routine embryo transfer techniques were used to establish recipient groups in which blastocysts were either asynchronous (blastocysts 24 h behind recipient uterus) or synchronous with their uterine environment. Oestradiol valerate (5 mg) was administered on Day 11 of the recipient's cycle to stimulate release of uterine secretion in the synchronous gilts (Group SE) and one group (AE) of asynchronous gilts. The gilts in the other asynchronous group (Group AC) were injected with vehicle (sesame oil). Embryos recovered on Day 14 by hysterectomy and flushing were evaluated for morphological development. Oestradiol treatment resulted in a failure of blastocyst development in Group AE gilts only. Recoverable oestradiol in the uterine flushings was increased in gilts in Groups AC and SE which contained elongated blastocysts. Plasmin inhibitor levels were lower in Groups AC and SE while PGF tended to be increased. Acid phosphatase activity was higher and recoverable Ca2+ was lower in Groups AE and SE. Failure of blastocyst development in Group AE is believed to have resulted from a failure to undergo trophoblastic elongation due to premature alteration of the uterine environment at a critical period of blastocyst development or from the presence of an unfavourable uterine environment for blastocyst attachment and development shortly after Day 12.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002118:Calcium / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D004958:Estradiol / Q000032:analysis / Q000494:pharmacology*; D005260:Female; D011247:Pregnancy; D011460:Prostaglandins F / Q000032:analysis; D011506:Proteins / Q000032:analysis; D013552:Swine / Q000502:physiology*; D013997:Time Factors; D014599:Uterus / Q000032:analysis / Q000502:physiology*
chemical_list: D011460:Prostaglandins F; D011506:Proteins; D004958:Estradiol; D000135:Acid Phosphatase; D002118:Calcium