Structural study of phosphomannan-protein complex of Citeromyces matritensis containing beta-1,2 linkage. Application of partial acid degradation and acetolysis techniques under mild conditions.
The phosphomannan-protein complex of Citeromyces matritensis IFO 0651 strain was investigated for its chemical structure by a sequential degradation procedure, partial acid degradation followed by acetolysis under mild conditions. Upon treatment with 10 mM HCl at 100 degrees C for 1 h, this complex released mannotriose and mannotetraose consisting solely of 1,2-linked beta-D-mannopyranosyl residues, ca. 20% on weight basis of the parent complex. The acid-degraded complex was then subjected to acetolysis using an acetolysis medium of low sulfuric acid concentration, a 100:100:1 (v/v) mixture of acetic anhydride, acetic acid, and sulfuric acid at 40 degrees C for 36 h. A phosphate-containing manno-oligosaccharide fraction eluted in the void-volume region of a Bio-Gel P-2 column was found to consist of Manp beta 1----2Manp beta 1----2Manp alpha 1----2Man to which 1 mol of phosphate group was attached, while a manno-oligosaccharide fraction eluted in the diffusable region was a mixture of Manp beta 1----2Manp beta 1----2Manp beta 1----2Manp alpha 1----2Man, Manp beta 1----2Manp beta 1----2Manp alpha 1----2Man, Manp beta 1----2Manp alpha 1----2Man, Manp alpha 1----2Man, and mannose in the molar ratio of 0.08:0.33:0.19:0.32:1.00. Therefore, the structural analysis of the polysaccharide moiety of a beta-1,2 linkage-containing phosphomannan-protein complex of fungal origin can be achieved by means of a sequential degradation procedure, partial acid degradation followed by acetolysis under mild conditions.
D000096:Acetone; D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D005656:Fungal Proteins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007120:Immunochemistry; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D011485:Protein Binding; D004718:Saccharomycetales / Q000032:analysis*
D000596:Amino Acids; D005656:Fungal Proteins; D008351:Mannans; D010710:Phosphates; D000096:Acetone; C005437:phosphomannan