Purification and biochemical characterization of streptothricin acetyltransferase coded by the cloned streptothricin-resistance gene of Streptomyces lavendulae.
Streptothricin acetyltransferase was purified from Streptomyces lividans harboring a plasmid which carried the streptothricin-resistance gene cloned from a streptothricin-producing strain, Streptomyces lavendulae No. 1080. Some properties of the enzyme were determined and the reaction product was identified to be N beta-acetylstreptothricin by NMR spectroscopy.
D000123:Acetyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
D000123:Acetyltransferases; C044496:streptothricin acetyltransferase