title: Na+/adenosine co-transport in Vibrio parahaemolyticus.
abstract: Adenosine transport in Vibrio parahaemolyticus was studied. Na+ greatly stimulated adenosine uptake. Addition of adenosine to a cell suspension under anaerobic conditions elicited Na+ uptake, and the Na+ uptake was inhibited by monensin, an Na+ ionophore. Imposition of an electrochemical potential of Na+ or a membrane potential in energy-depleted cells elicited adenosine uptake. Therefore, adenosine transport in this organism was concluded to proceed by an Na+/adenosine co-transport mechanism. The Na+/adenosine co-transport system was induced when cells were grown in the presence of adenosine, and repressed by glucose. Although Na+ uptake elicited by adenosine was reduced by glucose, it was enhanced by methyl alpha-glucoside, which reduced the intracellular ATP level. Thus, the effects of glucose and the glucoside on the Na+/adenosine co-transport system did not seem to be due to inducer exclusion, but to be related to the intracellular ATP level.
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D008757:Methylglucosides / Q000494:pharmacology; D008985:Monensin / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D014733:Vibrio / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D008757:Methylglucosides; D008985:Monensin; D012964:Sodium; D005947:Glucose; D000241:Adenosine