Replicative intermediate of hepatitis B virus in transfected murine fibroblasts.
The NIH 3T3-derived cell line psi AM22b, which carries a defective Moloney murine leukemia virus, was transfected with a plasmid carrying the neo gene and two head-to-tail copies of the hepatitis B virus (HBV) genome positioned with opposing polarities. Both the two HBV dimers and the neo gene were located between two Moloney murine leukemia virus long terminal repeats. Poly(A)+ RNAs isolated from one clone that grew in the presence of G418 contained the two major classes of HBV-specific transcripts (3.5-kilobase pregenome and 2.1-kilobase mRNAs) in approximately equivalent amounts, which was reminiscent of the profiles of viral mRNAs from the livers of infected humans and chimpanzees.
D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D051379:Mice; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014162:Transfection; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral