title: The intracellular pathway of antagglutinin secretion in the boar caput epididymidis as revealed by immunogold labelling.
abstract: Antagglutinin, a specific protein synthesized by the boar epididymis, was localized by an ultrastructural immunogold-labeling procedure in the principal cells of the three regions of the caput epididymidis, most notably at the sites of synthesis and secretion. The intensity of the reaction was variable in the three epididymal zones. Labeling was of low intensity in the proximal and middle caput, except in the granules of the latter. These granular "storage sites" did not correspond to typical secretory granules but appeared to be intracellular sites of degradation of this protein. In the distal caput, which was devoid of these granules, intense secretory activity for antagglutinin was detected. Few gold particles were localized in the RER profiles but labeling was detected in the Golgi zone, in numerous dense vesicles, in structures distributed between the Golgi zone and the apex of the cell, and in the epididymal lumen. This study has enabled us to visualize immunocytochemically antagglutinin along its intracellular secretory pathway, i.e. at the site of its synthesis, during its passage via the Golgi zone, and its intracellular transport to the lumen.
mesh_terms: D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D004822:Epididymis / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D006046:Gold; D008297:Male; D008854:Microscopy, Electron; D009928:Organ Specificity; D013552:Swine; D013735:Testicular Hormones / Q000032:analysis / Q000378:metabolism*
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D013735:Testicular Hormones; C053626:antagglutinin (boar); D006046:Gold