title: The influence of dietary fat on the lipogenic activity and fatty acid composition of rat white adipose tissue.
abstract: The in vivo fatty acid synthesis rate, selected enzyme activities and fatty acid composition of rat white adipose tissue from animals fed semisynthetic diets of differing fat type and content were studied. All animals were starved for 48 hr and then refed a fat-free (FF) diet for 48 hr. They were then divided into three groups. One group was continued on the FF diet for 48 hr. Another group was fed a diet containing 44% of calories from corn oil (CO). The final group was fed a diet containing 44% of calories from completely hydrogenated soybean oil (HSO). The animals on the FF diet had a marked increase in adipose tissue fatty acid synthesis during the 96-hr feeding period (as measured by 3H incorporation into adipose fatty acids). Addition of either CO or HSO to the diets did not significantly inhibit fatty acid synthesis in dorsal or epididymal adipose tissue. The activities of the enzymes' fatty acid synthetase, ATP-citrate lyase and glucose-6-phosphate dehydrogenase increased on the FF diet and generally were not inhibited significantly by the addition of either fat to the diets. Linoleic acid was the major polyunsaturated fatty acid (ca. 22%) in adipose tissue. Monounsaturated fatty acids (palmitoleic, oleic, cis-vaccenic) made up ca. 38% of the total adipose fatty acids, while saturated fatty acids accounted for about 32% (myristic, palmitic and stearic). White adipose tissue in mature male rats was a major depot for n-3 fatty acids.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism; D007652:Oxo-Acid-Lyases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005227:Fatty Acids; D008055:Lipids; D009097:Multienzyme Complexes; D064429:Fatty Acid Synthases; D007652:Oxo-Acid-Lyases; C019909:citrate (pro-3S)-lyase