title: Corneal antibody levels to ribitol teichoic acid in rabbits immunized with staphylococcal antigens using various routes.
abstract: Although Staphylococcus aureus is an important cause of infectious diseases of the eye and hypersensitivity lesions of the cornea, little is known about ocular immunity to this pathogen. Using an enzyme-linked immunosorbent assay, we measured antibody titers to ribitol teichoic acid, the major antigenic determinant of S. aureus, in corneas as well as serum and tears after immunizing rabbits using the following routes: intradermal injection of cell wall mixed with complete Freund's adjuvant, subconjunctival injection of cell wall mixed with complete Freund's adjuvant, topical application of cell wall to the eye or topical application of viable S. aureus to the eye. IgG titers to ribitol teichoic acid were found consistently in corneas after intradermal and subconjunctival immunization with cell wall and topical immunization with viable S. aureus. After intradermal immunization with cell wall, IgG titers in cornea were higher than tears but lower than serum, which was presumably the source of the IgG antibodies for the cornea. After subconjunctival immunization with cell wall or topical immunization with viable S. aureus, IgG titers in corneas were higher than tears and generally higher than serum, suggesting that the ocular tissues were a local source of IgG. On the other hand, IgA titers to ribitol teichoic acid were found in tears but not in serum and were found only occasionally in corneas, suggesting that IgG responses to staphylococcal antigens may be more important than IgA responses in the cornea. The results of this study suggest that corneal antibodies to ribitol teichoic acid may be influenced by exposure to staphylococcal antigens not only in the external eye but also at sites remote from the eye.
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000942:Antigens, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D003228:Conjunctiva; D003315:Cornea / Q000276:immunology* / Q000378:metabolism; D005260:Female; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood / Q000378:metabolism; D007267:Injections; D007271:Injections, Intradermal; D011817:Rabbits; D013211:Staphylococcus aureus / Q000276:immunology*; D013666:Tears / Q000378:metabolism; D013682:Teichoic Acids / Q000276:immunology*
chemical_list: D000906:Antibodies; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D013682:Teichoic Acids; C044340:ribitol teichoic acid