title: Analysis of monoamines and their metabolites by high performance liquid chromatography with coulometric electrochemical detection.
abstract: High performance liquid chromatography with coulometric electrochemical detection has been used to achieve simultaneous determination of norepinephrine, epinephrine, 5-hydroxytryptophan, normetanephrine, dopamine, metanephrine, 3,4-dihydroxyphenylacetic acid, N-acetyldopamine, tyramine, tryptophan, 5-hydroxyindoleacetic acid, 5-hydroxytryptamine, N-acetyl-5-hydroxytryptamine, homovanillic acid, tyrosine, p-octopamine, N-acetyl-p-octopamine, and p-synephrine. The procedure has been applied to study monoamine degradation in the insect brain and to demonstrate that N-acetylation rather than oxidative deamination is the primary route of monoamine catabolism in insects.
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003058:Cockroaches; D004563:Electrochemistry
chemical_list: D001679:Biogenic Amines