Use of individual human follicles to compare oocyte in vitro fertilization to granulosa cell in vitro luteinization.
Granulosa-lutein (G-L) cells from individual follicles aspirated during cycles of in vitro fertilization-embryo transfer were examined after 3 and 6 days in culture. G-L cells from follicles that contained an oocyte that fertilized in vitro were compared with G-L cells from follicles that contained an oocyte that did not fertilize in vitro. Spent culture media was assayed for progesterone at days 3 and 6 of culture and luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor content of G-L cells was determined at day 6. G-L cell cultures from follicles that contained an oocyte that fertilized in vitro produced significantly more progesterone over 3 and 6 days of culture than those obtained from follicles in which the oocyte did not fertilize. Furthermore, LH/hCG receptor content after 6 days was significantly higher in G-L cells obtained from follicles with fertilized oocytes compared with follicles with unfertilized oocytes. Increased progesterone output and LH/hCG receptor acquisition demonstrate more maturation or "luteinization" by G-L cells aspirated from individual follicles that contain oocytes that fertilized in vitro.
D002478:Cells, Cultured; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000166:cytology*; D006801:Humans; D007986:Luteinizing Hormone / Q000502:physiology*; D009865:Oocytes / Q000166:cytology*; D006080:Ovarian Follicle / Q000166:cytology*; D011374:Progesterone / Q000097:blood; D011974:Receptors, LH / Q000032:analysis
D011974:Receptors, LH; D011374:Progesterone; D007986:Luteinizing Hormone