Rapid extraction of anti-inflammatory drugs in whole blood for HPLC analysis.
A rapid and efficient procedure is described for the extraction and analysis of anti-inflammatory drugs in whole blood. Red blood cells were fragmented by sonication and the blood sample extracted by passing through a bonded silica column (Bond-Elut). The adsorbed drugs were washed and eluted followed by analysis by HPLC. Recoveries were in excess of 80% at 5 micrograms/ml concentrations.
D000082:Acetaminophen / Q000097:blood; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005279:Fenoprofen / Q000097:blood; D005554:Forensic Medicine; D006801:Humans; D007052:Ibuprofen / Q000097:blood; D007213:Indomethacin / Q000097:blood; D007660:Ketoprofen / Q000097:blood; D009288:Naproxen / Q000097:blood; D012459:Salicylates / Q000097:blood; D020156:Salicylic Acid
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012459:Salicylates; D000082:Acetaminophen; D009288:Naproxen; D007660:Ketoprofen; D020156:Salicylic Acid; D005279:Fenoprofen; D007052:Ibuprofen; D007213:Indomethacin