Influence of ornithine on albumin synthesis by fetal and neonatal hepatocytes maintained in culture.
Fetal rat as well as fetal and neonatal human hepatocytes were cultured in an arginine-free medium with or without L-ornithine. This amino acid was found greatly to improve cell survival of both rat and human parenchymal cells and to favour their proliferation. In addition, L-ornithine appeared to modulate the expression of various functions and particularly to increase albumin secretion. Since a correlated increase of the corresponding mRNA was observed, it may be postulated that L-ornithine acts at a pre-translational level.
D000418:Albumins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D009952:Ornithine / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000418:Albumins; D012333:RNA, Messenger; D004247:DNA; D009952:Ornithine