A sandwich ELISA for bovine serum in viral vaccines.
A double antibody sandwich ELISA for the detection of bovine serum in viral vaccines was developed and standardized with commercially available reagents. The detection limit by ELISA was 0.5 ng ml-1. ELISA was found to be 50-400 times more sensitive than the currently used assays. It was concluded that ELISA is a specific, sensitive and reproducible method for the determination of residual amounts of bovine serum in viral vaccines.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002417:Cattle; D004340:Drug Contamination / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D007122:Immunoelectrophoresis; D014613:Vaccines, Attenuated / Q000032:analysis / Q000592:standards; D014765:Viral Vaccines / Q000032:analysis / Q000592:standards*
D001798:Blood Proteins; D014613:Vaccines, Attenuated; D014765:Viral Vaccines