title: Import of the carboxy-terminal portion of acyl-CoA oxidase into peroxisomes of Candida tropicalis.
abstract: We report the sequence of a cDNA clone that codes for the carboxy-terminal portion of the peroxisomal protein, acyl-CoA oxidase, from the yeast, Candida tropicalis. This is a newly identified acyl-CoA oxidase sequence, most likely a second allele of POX4. The cDNA clone was expressed by in vitro transcription followed by translation. The major product, a 43-kD protein, associated with isolated peroxisomes in an in vitro import assay. More than half of the peroxisome-associated protein was protected from added protease, implying that it was internalized within the organelle. These findings indicate that there is sufficient information in the carboxy-terminal portion of the protein to target it to peroxisomes.
mesh_terms: D042962:Acyl-CoA Oxidase; D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002175:Candida / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005796:Genes; D008830:Microbodies / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D004247:DNA; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase