title: Transport of methionine hydroxy analog across the brush border membrane of rat jejunum.
abstract: Brush border membrane vesicles and everted rings of rat jejunum were used to study the absorption of 2-hydroxy-4-methylthiobutyric acid, the alpha-hydroxy analog of methionine (DL-MHA). Uptake by membrane vesicles was found to be unaltered by an inwardly directed sodium gradient in contrast to that of L-methionine, D-methionine and L-lactate, which is sodium stimulated and exhibits a characteristic "overshoot" phenomenon. Initial uptake rates of both L- and D-methionine (0.77 mM) were respectively 6.8 and 1.5 times higher than that of DL-MHA (0.80 mM) under Na+-gradient conditions. However, the hydroxy analog significantly inhibited L-lactate accumulation into membrane vesicles. Uptake of DL-MHA into rat jejunal rings was the sum of a saturable Michaelian component [KappM = 4.4 mM and Vappmax = 587 nmol/(g X min)] and a diffusive component [Kd = 47 microL/(g X min)]. Furthermore, L-lactate completely inhibited the carrier-mediated uptake of DL-MHA. All this demonstrates that jejunal uptake of DL-MHA in the rat is mediated by an Na+-independent carrier system associated with L-lactate transport and thus is quite distinct from the L-methionine pathway. This difference could help explain numerous findings with respect to a lower biological utilization of the hydroxy analog in some species when fed as the sole source of methionine in purified crystalline amino acid diets.
mesh_terms: D000818:Animals; D001692:Biological Transport; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D009940:Organoids / Q000378:metabolism; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D007773:Lactates; C008391:alpha-hydroxy-gamma-methylmercaptobutyric acid; D008715:Methionine