Reassembly of nucleosomal histone octamers during replication of chromatin.
We have examined critically whether or not new and old histones mix in the octameric units of nucleosomes during chromatin replication. MH-134SC cells were density-labeled by culturing with amino acid mixtures enriched with dense isotopes 2H, 13C, and 15N. Mononucleosomes obtained from labeled cells were fractionated by rate zonal sedimentation through a sucrose gradient in heavy water (Senshu et al. (1985) Eur J. Biochem. 150, 575-580). The fractionation can be performed under conditions that do not destabilize nucleosomes. Density-labeling yielded heterogeneous mononucleosome species which showed higher sedimentation rates than normal mononucleosomes. However, they were indistinguishable with respect to their protein compositions, electrophoretic mobilities, electrophoretic patterns of single-stranded DNA fragments liberated by DNase I digestion, electrophoretic mobilities and sedimentation velocities of the DNA moieties, and metabolic stabilities of the histone moieties. These data suggest that the heterogeneity of density-labeled mononucleosomes resulted from the formation of histone octamers density-substituted to different degrees. This would be an inevitable consequence of mixing of new and old histones in the octameric unit of nucleosomes.
D002478:Cells, Cultured; D002498:Centrifugation; D002843:Chromatin / Q000648:ultrastructure*; D004261:DNA Replication; D006657:Histones / Q000502:physiology*; D046911:Macromolecular Substances; D009024:Morphogenesis; D009707:Nucleosomes / Q000648:ultrastructure*
D002843:Chromatin; D006657:Histones; D046911:Macromolecular Substances; D009707:Nucleosomes