Scanning acoustic microscopy visualizes cytomechanical responses to cytochalasin D.
The reactions of XTH-2 cells (line derived from Xenopus laevis tadpole hearts) to cytochalasin D (CD) were followed using scanning acoustic microscopy (SAM) at 0.9 GHz, fluorescence and electron microscopy. The first reaction to CD which can be detected by SAM is a loss of image contrast, indicating a decrease in acoustic impedance of about 30%. Based on structural changes revealed by staining of actin with TRITC-phalloidin, and taking theoretical considerations into account, a relationship between impedance decrease and tension in the actin fibrillar system is deduced.
D000199:Actins / Q000032:analysis; D000818:Animals; D002460:Cell Line; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology*; D004727:Endothelium / Q000187:drug effects / Q000648:ultrastructure*; D006321:Heart / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D009206:Myocardium / Q000648:ultrastructure; D014982:Xenopus laevis
D000199:Actins; D003572:Cytochalasins; D015638:Cytochalasin D