[The effect of natural and recombinant leukocyte interferons on DNA repair and the formation of chromosome aberrations induced by N-methyl-N'-nitro-N-nitrosoguanidine].
The anticlastogenic action of natural leukocyte and recombinant (alpha 2) interferons was studied in human lymphocyte cultures treated with N-methyl-N'-nitro-N-nitrosoguanidine. The criteria of cell viability, proliferation, chromosome aberrations, frequency of micronucleus formation, formation and repair of DNA breaks were used for estimation of interferons activity. Reduction of the induced chromosomal aberrations was obtained in cells pretreated with interferons. The protective effect of natural leukocytic interferon was more expressed as compared with the effect of recombinant (alpha 2) interferon. The natural interferon was also more efficient than the recombinant one in DNA breaks formation and repair.
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008769:Methylnitronitrosoguanidine; D008938:Mitosis / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology
D007370:Interferon Type I; D011994:Recombinant Proteins; D008769:Methylnitronitrosoguanidine; D004247:DNA
Izuchenie deĭstviia prirodnogo i rekombinantnogo leĭkotsitarnykh interferonov na reparatsiiu DNK i formirovanie khromosomnykh aberratsiĭ, indutsirovannykh N-metil-N'-nitro-N-nitrozoguanidinom.