[Injection of glyoxylate ions from the recording microelectrode for the fluorescent microscopic detection of neuronal monoamines].
To make it sure that a physiological experiment is carried out on the monoaminergic neurone, we propose to fill the intracellular recording glass microelectrode with 2 M solution of potassium glyoxylate, with pH 6.0. After the experiment is over, glyoxylate ions are injected ionophoretically into the neurone, and then the specimen is dried in the air and heated to obtain fluorescence of a cellular monoamine, if it is present in the cell. Some details of the method are described.
D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D006038:Glyoxylates; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D007478:Iontophoresis; D008839:Microelectrodes; D008856:Microscopy, Fluorescence / Q000379:methods; D008974:Mollusca; D009474:Neurons / Q000166:cytology* / Q000378:metabolism
D001679:Biogenic Amines; D006038:Glyoxylates
In''ektsiia glioksilat-ionov iz registriruiushchego mikroélektroda dlia liuminestsentno-mikroskopicheskogo vyiavleniia neĭronal'nykh monoaminov.