Formation and utilization of acetoin, an unusual product of pyruvate metabolism by Ehrlich and AS30-D tumor mitochondria.
[14C]Pyruvate was rapidly non-oxidatively decarboxylated by Ehrlich tumor mitochondria at a rate of 40 nmol/min/mg of protein in the presence or absence of ADP. A search for decarboxylation products led to significant amounts of acetoin formed when Ehrlich tumor mitochondria were incubated with 1 mM [14C] pyruvate in the presence of ATP. Added acetoin to aerobic tumor mitochondria was rapidly utilized in the presence of ATP at a rate of 65 nmol/min/mg of protein. Citrate has been found as a product of acetoin utilization and was exported from the tumor mitochondria. Acetoin has been found in the ascitic liquid of Ehrlich and AS30-D tumor-bearing animals. These unusual reactions were not observed in control rat liver mitochondria.
D000093:Acetoin / Q000378:metabolism*; D000818:Animals; D002074:Butanones / Q000378:metabolism*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002849:Chromatography, Gas; D002951:Citrates / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats; D013053:Spectrophotometry
D002074:Butanones; D002951:Citrates; D011773:Pyruvates; D000093:Acetoin