Effect of acute 3,3'-dichlorobenzidine administration on rat hepatic enzymic and nonenzymic microsomal lipid peroxidation and antioxidant status.
The effect of pretreatment with 3,3'-dichlorobenzidine (DCB) on (i) enzymic and nonenzymic lipid peroxidation in hepatic microsomes--as measured by malondialdehyde (MDA) formation, and (ii) hepatic antioxidant status--as measured by the contents of vitamin E and reduced glutathione (GSH) and the activity of glutathione peroxidase, was examined in the rat. DCB-pretreatment (20 mg/kg/day, ip, for 2 days) caused an increase in NADPH-dependent (enzymic) lipid peroxidation, but had no effect on the linoleic acid hydroperoxide-dependent (nonenzymic) lipid peroxidation in microsomes. DCB-pretreatment also caused a 44% decrease in the content of vitamin E in microsomes, but had no effect on the content of GSH or the activities of glutathione peroxidases in the liver. The DCB-induced increase in in vitro microsomal lipid peroxidation is interpreted as resulting from the diminution of vitamin E induced by DCB in vivo.
D015101:3,3'-Dichlorobenzidine / Q000494:pharmacology*; D000818:Animals; D001560:Benzidines / Q000494:pharmacology*; D004347:Drug Interactions; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology
D001560:Benzidines; D008054:Lipid Peroxides; D014810:Vitamin E; D015101:3,3'-Dichlorobenzidine; D005978:Glutathione