title: Carboquone alters the protein synthesis of Chinese hamster V79 cell.
abstract: The effect of carboquone (CQ) on protein synthesis in Chinese hamster V79 cells was determined. While the syntheses of higher molecular weight proteins decreased, the relative level of a 43-kilodalton (kd) protein increased following exposure to CQ at the concentration of 0.5 microgram/ml, in a time-related fashion, determined using one-dimensional gel electrophoresis. Using two-dimensional gel electrophoresis, the 43-kd protein may be actin protein, and the syntheses of another two proteins of 8.5/45 and 4.8/270 (designated isoelectric point/molecular weight, kd) were increased in the CQ-treated V79 cells. These changes of the proteins induced by CQ in V79 cells were noted in HeLa cells. Three CQ-induced proteins are candidates for the elucidation of the antineoplastic effect of CQ.
mesh_terms: D000818:Animals; D001345:Autoradiography; D001389:Azirines / Q000494:pharmacology*; D002225:Carbazilquinone / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D014176:Protein Biosynthesis
chemical_list: D001389:Azirines; D002225:Carbazilquinone