title: [Comparative evaluation of the diagnostic value of indirect and reverse ELISA for demonstrations of specific IgM antibodies in toxoplasmosis in man].
abstract: Two ELISA techniques were investigated comparatively regarding their IgM sensitivity and specificity in human toxoplasmosis. Results of the fractionated Toxo-IgM-IIF assay were used as a standard for comparison. In 96 of 264 specimens the Toxo-IgM-IIF assay was positive. Positive sera were investigated quantitatively, negative sera only in the serum dilution 1/50 in both the indirect and the reverse Toxo-IgM ELISA. Sensitivity of the indirect technique was calculated greater than 99% and specificity 97%. The corresponding values for the reverse technique were 66% for sensitivity and greater than 99% for specificity.
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008832:Microchemistry; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology*
chemical_list: D007075:Immunoglobulin M
vernacular_title: Vergleichende Untersuchungen über den diagnostischen Wert des indirekten und des reversen ELISA zum Nachweis spezifischer IgM-Antikörper bei der Toxoplasmose des Menschen.