Fractionation of human milk oligosaccharides by high-performance liquid chromatography.
An ion-exchange chromatographic system was used to isolate several human milk oligosaccharides, the elution being carried out with a linear gradient of a sodium borate buffer. Lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose I, II and III, lacto-N-difucohexaose I and 2'-alpha-fucosyllactose can be separated by this method.
D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008895:Milk, Human / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*
D009844:Oligosaccharides