title: Increased prolactin binding and morphological changes in the wound epithelium of regenerating limbs of Notophthalmus viridescens.
abstract: Distribution of prolactin has been examined in regenerating forelimbs from the newt Notophthalmus viridescens. Specific prolactin binding was demonstrated in homogenates of unamputated tissue, and of regenerating limbs at from 3 to 21 days postamputation. Labeled prolactin that was injected intraperitoneally into animals with one regenerating limb accumulated in the most distal portion of the regenerate at 7 and 14 days postamputation. Light-microscopic autoradiography demonstrated that labeled prolactin was localized most heavily in the apical, outer layer of the wound epithelium. Scanning electron microscopy demonstrated that, in addition to changes in prolactin affinity following amputation, morphological changes occurred in the apical wound epithelium as well. Cell surfaces of the stump epidermis were characterized by periodic dispersion of papillae among a network of interconnecting structures 1-2 microns across. By contrast, the surfaces of cells from the area in which labeled prolactin was found to localize most intensely were characterized by lack of papillae and, depending on the stage of regeneration, a pattern of microvilli and microplicae. These morphological alterations appear to reflect functional and biochemical differences between stump epidermis and wound epithelium.
mesh_terms: D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005552:Forelimb; D008855:Microscopy, Electron, Scanning; D011388:Prolactin / Q000378:metabolism*; D011981:Receptors, Prolactin / Q000378:metabolism*; D012038:Regeneration; D012455:Salamandridae; D014947:Wounds and Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D011981:Receptors, Prolactin; D011388:Prolactin