Immunolabelling of freeze-fractured tissue: thin sections of rat aorta after labelling of elastic laminae.
Rat aortae were fixed, reduced into fragments, fixed again according to the fracture-label method from Pinto da Silva et al. (1981) and labelled with anti-elastin antibodies. We thus demonstrate that this method, extensively used with lectins, is also usable with antibodies to investigate components of the extracellular matrix.
D000818:Animals; D001011:Aorta / Q000648:ultrastructure*; D004549:Elastin / Q000032:analysis* / Q000276:immunology; D005614:Freeze Fracturing; D006046:Gold; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000648:ultrastructure*; D051381:Rats
D006046:Gold; D004549:Elastin