Monoclonal and polyclonal antibodies compared for radioimmunoassay of somatomedin-C in patients with acromegaly or hypopituitarism.
We used a synthetic recombinant analog of somatomedin-C (Sm-C) to directly compare the performance of polyclonal and monoclonal antibodies for measuring Sm-C in serum of normal persons and in acromegalic or hypopituitary patients. Mean concentrations of Sm-C in healthy adults were 181 (SD 42) micrograms/L as measured with the monoclonal antibody, 194 (SD 61) micrograms/L with the polyclonal antibody. Both antisera gave excellent discrimination between acromegalics and normals. However, the assay with the polyclonal antibody was more sensitive than that with the monoclonal antibody (lower detection limits: 5 vs 100 micrograms/L) and thus better suited for quantifying Sm-C in samples from hypopituitary patients.
D000172:Acromegaly / Q000097:blood* / Q000175:diagnosis; D000328:Adult; D000911:Antibodies, Monoclonal; D006801:Humans; D007018:Hypopituitarism / Q000097:blood* / Q000175:diagnosis; D007334:Insulin-Like Growth Factor I / Q000097:blood*; D011786:Quality Control; D011863:Radioimmunoassay; D013002:Somatomedins / Q000097:blood*
D000911:Antibodies, Monoclonal; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I