The effect of exogenous glutathione on the fructolysis of thawed bull Bos bovis spermatozoa.
1. The effect of glutathione (5mM) addition to the diluent used for sperm preservation on fructolysis and motility of bull spermatozoa was studied. 2. Glutathione had no effect on lactate and pyruvate concentration and on the motility of spermatozoa immediately after their thawing. 3. During 3 hr incubation at 37 degrees C glutathione decreased the pyruvate formation, significantly increased the lactate production and prevented the decrease in the number of spermatozoa with maintained progressive movement.
D000818:Animals; D002417:Cattle; D005632:Fructose / Q000378:metabolism*; D005978:Glutathione / Q000494:pharmacology*; D007773:Lactates / Q000096:biosynthesis; D008297:Male; D011309:Preservation, Biological / Q000662:veterinary*; D011773:Pyruvates / Q000096:biosynthesis; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
D007773:Lactates; D011773:Pyruvates; D005632:Fructose; D005978:Glutathione