title: Identification of the proteins in direct contact with duck globin mRNA.
abstract: Proteins in direct contact with translationally active and repressed duck globin mRNA were determined by irradiating blood or lysates with ultraviolet light. Cross-linked proteins from polyribosomes and free mRNP particles were 14C-labeled by reductive methylation and identified on SDS-polyacrylamide gels upon autoradiography. Results indicate that ten cross-linked proteins are common to both polysomal and free mRNP, however, a 44 kDa protein appears to be specific for repressed mRNP particles. Furthermore, the notable lack of cross-linked proteins in the 20-30 kDa range in free mRNP supports the view that the characteristic low molecular mass 'prosomal' proteins, previously found associated with translationally repressed duck globin free mRNP [(1984) EMBO J. 3, 29-34], do not interact directly with the mRNA molecule.
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D004372:Ducks; D005914:Globins / Q000235:genetics* / Q000302:isolation & purification; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D012156:Reticulocytes / Q000378:metabolism* / Q000528:radiation effects; D012261:Ribonucleoproteins / Q000097:blood / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D001798:Blood Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D005914:Globins