A method of oxygen supply to a low volume tissue bath containing a protein solution.
A method that makes it possible to follow the in vitro activity of a ring of the rabbit ear artery submerged in a protein solution for hours is described. The necessary oxygen and carbon dioxide are supplied to the tissue bath through a semipermeable membrane from a pressure chamber. By this procedure, disturbing foam formation is avoided. The tissue bath has a volume of 5 ml. For the rabbit ear artery, it is demonstrated that the response to electrical field stimulation is influenced by the addition of albumin.
D000818:Animals; D004423:Ear / Q000098:blood supply; D004558:Electric Stimulation; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D010477:Perfusion / Q000295:instrumentation*; D011506:Proteins; D011817:Rabbits; D012996:Solutions
D011506:Proteins; D012996:Solutions; D010100:Oxygen