Cardiovascular tissue phospholipid metabolism in the Dahl rat: influence of dietary salt.
Cardiac and thoracic aortic tissue were removed from 8-9 week old Dahl salt resistant (DR) and salt sensitive (DS) rats following 2 and 4 weeks of 8% NaCl diets. The tissue was used to determine the pool size and rate of [32P] Pi incorporation into phosphatidylinositol-4-5-bisphosphate (PIP2), phosphatidylinositol-4-phosphate (PIP) and phosphatidic acid (PA). The studies show no strain differences in the pool size of these phospholipids nor any changes in pool size as a consequence of the duration of exposure to the 8% NaCl diet. However, [32P] Pi incorporation into PIP2, PIP and PA was increased in the cardiac tissue isolated from both DR and DS rats exposed to 4 versus 2 weeks of 8% NaCl diet prior to sacrifice. The relative increase was comparable in both strains. Further, the extent of [32P] Pi incorporation into these phospholipids was also increased in the aorta of DR, but not DS, rats exposed to 8% NaCl diets for 4 versus 2 weeks. The present study defines a strain specific difference in aortic tissue response to prolonged 8% NaCl diet exposure.
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002319:Cardiovascular System / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology*; D013045:Species Specificity
D010743:Phospholipids; D012965:Sodium Chloride