A rapid enzyme-linked semi-microwell assay for the enumeration of antibody-forming cells to viral and bacterial antigens in domestic animals.
Details of an enzyme immunoassay for the enumeration of antibody-forming cells are given with examples of its application to immunization with viral (Newcastle disease virus) or bacterial (Haemophilus pleuropneumoniae) pathogens of domesticated species. The assays showed reciprocal specificity when compared to another virus (avian paramyxovirus serotype 3) or another bacterium (Bordetella bronchiseptica) and were used to show the class specificity of avian AFC and the effect of passive antibody transfer on porcine AFC induction.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D006190:Haemophilus / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009522:Newcastle disease virus / Q000276:immunology; D013552:Swine
D000942:Antigens, Bacterial; D000956:Antigens, Viral