Rat liver mitochondria contain two immunologically distinct dihydrolipoamide dehydrogenases.
We have raised antisera against dihydrolipoamide dehydrogenase. One antigen was isolated from purified bovine kidney pyruvate dehydrogenase complex (PDC). The other antigen was a commercial preparation of porcine heart dihydrolipoamide dehydrogenase (E3) which did not first involve purification of the alpha-keto acid dehydrogenase complex(es). Both antibody preparations cross-reacted with the E3 components of PDC, alpha-ketoglutarate dehydrogenase complex, and branched-chain keto acid dehydrogenase complex. This demonstrates the immunological identity of the E3 components. These sera totally precipitated E3 activity from the purified complexes, from purified preparations of E3, and from extracts of rat heart and kidney mitochondria. The two sera vary in their reaction with rat liver mitochondrial extracts: the anti PDC-E3 serum left residual E3 activity (approximately 50% of the original) that was precipitable by the anti-E3 anti-serum. This indicates that liver contains two immunologically distinct forms of E3. Metabolic assays measuring the differential effects of the two sera on the glycine decarboxylation reaction suggest that the form which is immunologically nonreactive with the anti-PDC-E3 serum could represent the E3 involved in the glycine cleavage system.
D000818:Animals; D000936:Antigen-Antibody Complex; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D007106:Immune Sera; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology; D007668:Kidney / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D011768:Pyruvate Dehydrogenase Complex / Q000032:analysis; D051381:Rats
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007527:Isoenzymes; D011768:Pyruvate Dehydrogenase Complex; D008058:Dihydrolipoamide Dehydrogenase