Purification and characterization of fish surface mucin.
Fish surface mucin from Pampus argenteus was extracted with different organic solvents and the residue passed through Sephadex G-200. The major peak was purified by DEAE-Cellulose chromatography and five fractions were obtained. Carbohydrate and protein contents showed that major peak is a glycoprotein. Rechromatography of this component on the Sephadex G-200 column gave a single peak, with an estimated minimal molecular weight of 6.9 X 10(5). Analysis of individual sugar components revealed the presence of galactose, glucose, mannose, arabinose, N-acetyl glucosamine, N-acetyl galactosamine and sialic acid. The most represented amino acids are threonine, serine, proline, glutamic acid and glycine. The N-terminal amino acid end was blocked. Nearly 47% of sulphate was acid labile. Sialic acid and fucose were released rapidly by mild acid hydrolysis. The presence of blood group-A activity suggests that some kind of terminal alpha-Gal-NAC may be present.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D005591:Chemical Fractionation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005399:Fishes / Q000378:metabolism*; D005643:Fucose / Q000378:metabolism; D006868:Hydrolysis; D009077:Mucins / Q000302:isolation & purification* / Q000378:metabolism; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D009077:Mucins; D012794:Sialic Acids; D013431:Sulfates; D005643:Fucose; D019158:N-Acetylneuraminic Acid