title: Modification of muscle proteins IEF patterns in a fish Lebistes reticulatus, related to diet, immunization and genetic selection.
abstract: The effects of some environmental and genetic factors on the isoelectric focusing (IEF) patterns of muscle proteins from a fish Lebistes reticulatus (Cyprinodonts) were investigated. Of two diets applied the restricted one resulted in a loss of bands in the IEF patterns of the treated fishes muscle homogenate. Instead immunization gave an increase in both number and intensity of IEF bands in (4.0-6.0; 7.0-7.9) pH ranges which are typical of immunoglobulins. Lastly IEF patterns from random and assortative mating animals were compared, resulting in loss of IEF-bands in the inbred lines. Results showed that IEF of muscle proteins could be an excellent method to evaluate the response by fish phenotypes to environmental or genetic factors.
mesh_terms: D000818:Animals; D003532:Cyprinodontiformes / Q000378:metabolism*; D004032:Diet; D006863:Hydrogen-Ion Concentration; D007114:Immunization / Q000662:veterinary*; D007525:Isoelectric Focusing; D009124:Muscle Proteins / Q000032:analysis*; D011036:Poecilia / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012641:Selection, Genetic
chemical_list: D009124:Muscle Proteins