Cytoskeletal characterization of a neuroectodermal cell line and embryonic mouse brain.
The cytoskeleton of a newly isolated mouse embryo brain-derived cell line and of dissected mesencephalon-rhombencephalon samples of the 10- to 11-day-old mouse embryo, constituted of a ventricular zone only, has been examined by immunocytochemistry, electrophoretic separation and Western blotting techniques. In accordance with their epithelial organization, the ventricular cells contain cytokeratins as main constituents of their cytoskeleton. Vimentin has been also revealed as early as day 10.5 of gestation. The complex cytokeratin polypeptide pattern puts this histological type of epithelia into the category of complex, rather than simple, epithelia and calls for explanations other than keratinization for the presence and functional role of the high-molecular weight, basic keratins. The cytoskeletal composition of the embryo brain-derived gliogenic cell line reflects the vimentin- and cytokeratin-positive character of the ventricular cells.
D000818:Animals; D001921:Brain / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D002454:Cell Differentiation; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000648:ultrastructure*; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009420:Nervous System / Q000166:cytology / Q000196:embryology* / Q000201:enzymology / Q000648:ultrastructure; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D013997:Time Factors
D003598:Cytoskeletal Proteins; D007527:Isoenzymes; D010751:Phosphopyruvate Hydratase