Pesticide clastogenicity in Chinese hamster ovary cells.
Paraquat, alachlor, butachlor, phorate and monocrotophos, several of the most extensively used pesticides in Taiwan, were investigated for their clastogenicity using chromosome aberration (CAb) induction in Chinese hamster ovary (CHO) cells. Significance levels of the binomial trend analysis and binomial mutagenicity data test were two criteria for the summary judgement of the pesticide clastogenicity. Except for phorate, all pesticides tested were clastogenic to CHO cells in the absence of in vitro metabolic activation by S9. 5 microliters/ml rat-liver extract, S9, were used as the source of in vitro metabolic activation. 3 different outcomes were found after the addition of S9. Paraquat: significant decrease in induced CAbs. Monocrotophos: concomitant occurrence of decreased cytotoxicity and increased clastogenicity. Alachlor, butachlor and phorate: increased cytotoxicities with no sign of enhancement in clastogenicity.
D000081:Acetamides / Q000633:toxicity; D000818:Animals; D001711:Biotransformation; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D008862:Microsomes, Liver / Q000378:metabolism; D008999:Monocrotophos / Q000633:toxicity; D009153:Mutagens; D010269:Paraquat / Q000633:toxicity; D010575:Pesticides / Q000633:toxicity*; D010702:Phorate / Q000633:toxicity; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013624:Taiwan
D000081:Acetamides; D009153:Mutagens; D010575:Pesticides; C000188:alachlor; D010702:Phorate; D008999:Monocrotophos; D010269:Paraquat