Determination of myoglobin in human serum by high-performance liquid chromatography with chemiluminescence detection.
A highly sensitive method for the determination of myoglobin in serum is described, based on high-performance size-exclusion chromatography with chemiluminescence detection. Serum proteins are separated according to their molecular masses on columns packed with TSK-SW gel and those containing haem are detected selectively by post-column chemiluminescence reaction with luminol using a conventional fluorimetric detector. The method is rapid (30 min) and sufficiently sensitive for the diagnosis of myocardial infarction. The minimum detectable myoglobin concentration is 10 ng/ml.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008970:Molecular Weight; D009203:Myocardial Infarction / Q000175:diagnosis; D009211:Myoglobin / Q000097:blood*; D011863:Radioimmunoassay
D007202:Indicators and Reagents; D009211:Myoglobin