Analysis of the integrant in MyK-103 transgenic mice in which males fail to transmit the integrant.
Males in the transgenic mouse pedigree MyK-103, although fertile, do not transmit the integrant to offspring. The integrant is on chromosome 6 near the T-cell receptor beta-chain locus. It contains four fragments of the plasmid pMK (a metallothionein-thymidine kinase fusion gene) and a 532-base-pair fragment of displaced mouse DNA originating from a previously uncharacterized repetitive DNA family. The integration complex is flanked on either side by a 5-kilobase duplication of mouse DNA normally found in a single copy at this locus. Sequence analysis of the six novel junctions and their donor sequences shows that plasmid-plasmid junctions occurred at patches of limited homology, whereas chromosome-plasmid junctions were nonhomologous.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D008668:Metallothionein / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013091:Spermatogenesis; D013737:Testis / Q000502:physiology*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection
D008668:Metallothionein; D013937:Thymidine Kinase