Effects of avian and mammalian serum on steroidogenesis in granulosa cells of the hen (Gallus domesticus).
Addition of serum (0.02-2%, v/v) from laying hens to dispersed granulosa cells isolated from the largest and second-largest ovarian follicles of regularly laying hens significantly potentiated both ovine- and chicken LH-induced progesterone production. A similar treatment with mammalian sera, on the other hand, tended to suppress steroidogenesis, though not to a statistically significant degree. When steroidogenesis was stimulated by forskolin or 8-bromo-cyclic AMP, both of which act at postreceptor sites, the presence of either avian or mammalian sera enhanced steroidogenesis over controls stimulated by agonist alone. It is suggested that serum lipoproteins potentiate steroidogenesis, but that factors present in mammalian sera interfere with the action of LH at a step preceding cyclic AMP generation.
D000818:Animals; D002645:Chickens / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006736:Horses / Q000097:blood; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology; D011374:Progesterone / Q000096:biosynthesis*; D011817:Rabbits / Q000097:blood; D051381:Rats / Q000097:blood; D012756:Sheep / Q000097:blood
D011374:Progesterone; D007986:Luteinizing Hormone