[Genetic transformation of somatic cells. XIV. Expression of gene coding for the human hepatitis B virus surface antigen in mammalian cells].
A set of recombinant plasmids with a gene encoding surface antigen of hepatitis B virus (HBsAg) is constructed. The plasmids were transfected by DEAE-dextran method into different lines of cultured cells and transient expression of the HBsAg gene was studied. The results indicate that: transcriptional enhancer of hepatitis B virus situated downstream from HBsAg gene is active in green monkey kidney cells (CVI), promoter of 5 LTR of bovine leukemia virus is trans-activated in the goat or calf cells infected with BLV. The results are discussed in the light of hypothesis on the role of transcriptional enhancers in determination of tissue-specificity of hepatitis B virus.
D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005815:Genetic Code; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D006514:Hepatitis B Surface Antigens
Geneticheskaia transformatsiia somaticheskikh kletok. XIV. Ekspressiia gena, kodiruiushchego poverkhnostnyĭ antigen virusa gepatita B cheloveka, v kletkakh mlekopitaiushchikh.