Regulation of low density lipoprotein metabolism by 26-hydroxycholesterol in human fibroblasts.
Oxygenated derivatives of cholesterol are believed to play a role in cellular cholesterol homeostasis through the feed-back control of its biosynthesis. We report that 26-hydroxycholesterol inhibits the specific binding, uptake and degradation of 125I-LDL in human fibroblasts. The effect is dose-dependent, and saturation kinetics indicates a reduction of LDL-binding sites with no effect on ligand affinity. The results support a possible role of 26-hydroxycholesterol, a physiological oxysterol, in the regulation of cellular cholesterol homeostasis.
D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis / Q000494:pharmacology; D005246:Feedback; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D012867:Skin / Q000166:cytology
D006888:Hydroxycholesterols; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008077:Lipoproteins, LDL; C007042:cholest-5-ene-3 beta,26-diol; D002784:Cholesterol