Short-term biological reproducibility of serum fatty acid composition in children.
To assess the biological day-to-day reproducibility of the fatty acid composition in serum lipid fractions in children on their habitual diets, fasting serum specimens were collected from healthy 8- to 9-year-old boys at 2-day (n = 21) or 7-day (n = 19) intervals. Percentage compositions of fatty acids in serum cholesteryl esters (CE), triglycerides (TG), free fatty acids (FFA) and phospholipids (PL) were analyzed by gas chromatography. The reproducibility was estimated by calculating linear correlation coefficients between the values obtained at the two time-points. Generally the correlations were higher in CE and PL than in TG and FFA. In CE the 7-day correlations of major fatty acids were only slightly lower than the 2-day ones. In PL the impairment of correlations was somewhat greater than in CE, but in TG and FFA there was a marked drop in reproducibility from 2- to 7-day intervals. The 2-day correlation coefficients of the percentages of linoleate, dihomo-gamma-linolenate, arachidonate and eicosapentaenoate in CE were 0.95, 0.84, 0.91 and 0.92, respectively. In PL, the respective values were 0.86, 0.82, 0.95 and 0.90. The present results indicate high short-term biological reproducibility of fatty acid composition in serum CE and PL and clearly lower in those of TG and FFA.
D002648:Child; D002788:Cholesterol Esters / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005069:Evaluation Studies as Topic; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D008297:Male; D010743:Phospholipids / Q000097:blood*
D002788:Cholesterol Esters; D005227:Fatty Acids; D010743:Phospholipids