[Method for the simultaneous determination of residues of chemotherapeutic agents, antiparasitic agents and growth promoters in food of animal origin. 3. Determination of chloramphenicol and meticlorpindol].
Chloramphenicol and meticlorpindol may be determined by HPLC with UV-detection with a limit of 0.02-0.03 mg/kg. Residues of 0.001 mg/kg Chloramphenicol are determined by Capillary-GC with an ECD after an additional clean up step using chromatography on a miniaturized silica column, followed by a silylation step. HMDS/TMCS/Pyridine forms the disilylated Chloramphenicol. In samples of eggs, milk and meat the limit of detection is roughly 0.0002 mg/kg and is adequate to check the FDR tolerance levels for these foods. The proposed method was checked with residues of chloramphenicol in the range of 0.0005 to 0.005 mg/kg in milk from animals dosed with chloramphenicol. Results obtained by this method and results of a RIA-method correlated very well. The analytical procedure (applicable for all substances) was abbreviated compared to the method reported in the first communication on this topic.
D000818:Animals; D002417:Cattle; D002701:Chloramphenicol / Q000031:analogs & derivatives / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003007:Clopidol / Q000031:analogs & derivatives / Q000032:analysis*; D005260:Female; D005504:Food Analysis; D008460:Meat / Q000032:analysis; D008832:Microchemistry; D008892:Milk / Q000032:analysis; D011725:Pyridines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D011725:Pyridines; D002701:Chloramphenicol; D003007:Clopidol
Multimethode zur Bestimmung der Rückstände von Chemotherapeutica, Antiparasitica und Wachstumsförderern in Lebensmitteln tierischer Herkunft. 3. Mitteilung: Bestimmung von Chloramphenicol und Meticlorpindol.