title: Elemental changes associated with chondrocyte differentiation in rat rib growth plate.
abstract: Quantitative X-ray microanalysis was under-taken to follow the elemental changes that occur in the process of chondrocyte differentiation. For analysis at the cellular level, semi-thick freeze-dried cryosections of rat rib growth plate cartilage were used. For evaluation of the elemental concentrations at the subcellular level, thin sections of freeze-dried and low temperature vacuum embedded cartilage were analyzed. Levels of Na, P, S, Cl, K, and Ca were determined in the cells and extracellular matrix in different zones of the cartilage--resting, proliferative, and hypertrophic. Proliferative cells had a sodium concentration that was twice that of resting cells, suggesting that Na may play an important role in the regulation of DNA- and protein-synthesis in chondrocytes, A concomitant rise in Na and S concentration occurred between resting zone and proliferative zone cartilage matrix. The high concentrations of Na and K in the matrix are probably due to the high amount of sulfate in proteoglycans which may bind these cations.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D004577:Electron Probe Microanalysis; D004602:Elements / Q000032:analysis*; D005612:Freeze Drying; D006132:Growth Plate / Q000032:analysis / Q000166:cytology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012272:Ribs / Q000032:analysis / Q000166:cytology
chemical_list: D004602:Elements