Effect of DFP on loading of fura 2/AM and quin 2/AM into single smooth muscle cells prepared from guinea pig taenia coli.
The effect of diisopropyl fluorophosphate (DFP), a potent cholinesterase (ChE) inhibitor, on loading quin 2 acetoxymethyl ester (quin 2/AM) and fura 2/AM into smooth muscle cells isolated from guinea pig taenia coli was investigated spectrofluorometrically. The presence of DFP during the loading permitted the incorporation of quin 2 into the cells, so that it became possible to measure intracellular Ca2+ concentrations using the ester of this dye. Also, DFP significantly enhanced the incorporation of fura 2 into the cells. These results indicate that loading of quin 2/AM and fura 2/AM into the smooth muscle cells may depend on the suppression of ChE or various serine protease activities outside cells.
D000634:Aminoquinolines / Q000378:metabolism*; D000818:Animals; D001572:Benzofurans / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D016257:Fura-2 / Q000031:analogs & derivatives*; D006168:Guinea Pigs; D006868:Hydrolysis; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects*
D000634:Aminoquinolines; D001572:Benzofurans; D005456:Fluorescent Dyes; C049925:fura-2-am; D007531:Isoflurophate; C035738:Quin2-acetoxymethyl ester; D002118:Calcium; D016257:Fura-2