[Incorporation, into the DNA, chain of a fluorescent derivative of 2-deoxyuridylic acid during catalysis of synthesis by DNA-polymerase A].
A 2'-deoxyuridine 5'-triphosphate analogue with a dansyl (5-dimethylaminonaphtalene 1-sulphonyl) residue in the 5-position of uracyl has been synthesised. This compound substitutes dTTP in the DNA synthesis catalyzed by Klenow's fragment of E. coli DNA polymerase I on the M13mp10 phage DNA as template with synthetic 14-member primer. When the synthesis is terminated by four termination substrates, structure of the synthesised DNA chain can be read. It demonstrates in principle possibility of determination of DNA sequence by means of fluorescence.
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000096:biosynthesis*; D004277:DNA, Single-Stranded; D004259:DNA-Directed DNA Polymerase; D003856:Deoxyuracil Nucleotides / Q000138:chemical synthesis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*
D004277:DNA, Single-Stranded; D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; C052975:5-(dansylglycyl-6-aminohexanoylaminoprop-1-enyl)-2'-deoxyuridine 5'-triphosphate; D004247:DNA; D004259:DNA-Directed DNA Polymerase
Vkliuchenie v tsep' DNK fluorestsentnogo proizvodnogo 2'-dezoksiuridilovoĭ kisloty pri katalize sinteza DNK-polimerazoĭ A.