Detection and quantitation of T-2 mycotoxin in rat organs by radioimmunoassay.
A standard radioimmunoassay was compared with radiochromatography for the ability to detect unlabeled T-2 mycotoxin in organs from exposed animals. When 10% of HT-2, the only known metabolite that cross-reacts with T-2, was included and expressed as T-2 equivalents in the radiochromatographic detection, correlation between toxin detection in liver, spleen, and kidney by the 2 techniques was r = 0.98. An unknown metabolite was detected in heart extract by radiochromatography. Inclusion of this material in the T-2 equivalents detected by radiochromatography indicated a near-perfect correlation (r = 0.95; p greater than 0.05; slope = 0.82; y = intercept = 72) among all 4 tissues.
D000818:Animals; D002606:Charcoal; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D011863:Radioimmunoassay; D051381:Rats; D011916:Rats, Inbred F344; D012717:Sesquiterpenes / Q000032:analysis*; D013605:T-2 Toxin / Q000032:analysis*; D014018:Tissue Distribution
D007202:Indicators and Reagents; D012717:Sesquiterpenes; D002606:Charcoal; D013605:T-2 Toxin