[Screening model of anticancer drugs against human cancer transplanted into the subrenal capsule of nude mice].
Xenografts of human tumors in nude mice have more advantages to evaluate drug response than conventional experimental models. However, this method has difficulties in establishing tumor growth and in evaluating drug response in short period. In order to evaluate drug response in short time and to apply it on clinical study, we examined a newly devised method of sensitivity test in which tumor tissues were inoculated subrenal capsules of nude mice. The drug doses were decided from LD50 values of nude mice. Drug effects were estimated ten to twelve days after tumor implantation. Subrenal capsule implants grew early without latent period in nude mice, but in BDF1 normal mice those were diminished and disappeared within four to seven days after inoculation. The results of drug response in this method almost agreed with the method of subcutaneous inoculation in which drug effects were estimated three weeks after tumor inoculation.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000473:pathology; D003114:Colony-Forming Units Assay; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000473:pathology; D014410:Tumor Stem Cell Assay
D000970:Antineoplastic Agents