Synthesis of porcine neuropeptide Y(NPY) in solution.
The porcine neuropeptide Y (NPY), a 36-residue peptide amide, was synthesized by assembling six peptide fragments followed by thioanisole-mediated deprotection with trifluoromethanesulfonic acid in trifluoroacetic acid. beta-Cycloheptyl aspartate, Asp(OChp), was employed to suppress base-catalyzed succinimide formation. When administered to dogs, the purified peptide (10 micrograms/kg) caused prolonged increase of systemic arterial blood pressure and decreased pancreatic blood flow.
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004285:Dogs; D007202:Indicators and Reagents; D008297:Male; D009478:Neuropeptide Y / Q000138:chemical synthesis* / Q000494:pharmacology; D009904:Optical Rotation; D010446:Peptide Fragments; D012996:Solutions; D013552:Swine
D007202:Indicators and Reagents; D009478:Neuropeptide Y; D010446:Peptide Fragments; D012996:Solutions