title: IgG subclass distribution of serum antibodies against lipopolysaccharide from Bacteroides gingivalis in periodontal health and disease.
abstract: Serum IgG1, IgG2, IgG3 and IgG4 antibody levels directed against lipopolysaccharide (LPS) from Bacteroides gingivalis were measured in the sera from systemically healthy subjects with and without periodontitis. An enzyme-linked immunosorbent assay was used that included coating of microtiter plates with LPS, and subsequent incubation with patient sera followed by mouse monoclonal subclass-specific antibodies, biotinylated sheep anti-mouse IgG and alkaline phosphatase conjugated to streptavidin. Anti-LPS IgG antibodies were dominated by IgG2, and moderate amounts only of IgG1, IgG3 and IgG4 were found. The periodontitis patients had significantly higher anti-LPS IgG1, IgG2 and IgG3 levels when compared to the subjects with healthy periodontium (p less than 0.05, Mann-Whitney test).
mesh_terms: D000907:Antibodies, Bacterial / Q000145:classification*; D001439:Bacteroides / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D008070:Lipopolysaccharides