title: Primary structure of eel (Anguilla japonica) growth hormone.
abstract: Two molecular forms of growth hormone, GHs I and II, were isolated from a culture medium of the eel pituitary (Anguilla japonica). The complete amino acid sequence of GH I was determined in the present study. The hormone was reduced, carboxymethylated, and subsequently cleaved with cyanogen bromide and enzymes. Intact eel GH I was also digested with enzyme. The resulting fragments were separated by reverse-phase high-performance liquid chromatography (HPLC) and subjected to sequence analysis by automated gas-liquid sequencer employing an Edman method. Eel GH I consists of 190 amino acid residues with two disulfide linkages formed between residues 52-163 and 180-188. Sequence comparison with other vertebrate GHs revealed that eel GH I is closer to avian and mammalian GHs with 55% identity than to salmon GH with 48% identity.
mesh_terms: D000595:Amino Acid Sequence; D000811:Anguilla; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D013006:Growth Hormone / Q000032:analysis / Q000302:isolation & purification*; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism
chemical_list: D013006:Growth Hormone