Functional antagonism by monoclonal antibody to digoxin in the guinea-pig atria.
A monoclonal antibody with a high affinity for digoxin (Ka = 1.14 X 10(9) M) was produced by somatic cell fusion. This antibody, designated 35/6.1, displayed little or no cross reactivity with other steroids. In the guinea-pig atria, 35/6.1 antagonized functionally the positive inotropic effect of digoxin but did not alter that of isoproterenol. Our results suggest that this monoclonal antibody may represent a useful probe for studying the physiological role of the putative endogenous digoxin-like factor in various pathophysiological conditions.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D004077:Digoxin / Q000037:antagonists & inhibitors* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects*; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009200:Myocardial Contraction / Q000187:drug effects; D012709:Serum Albumin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012709:Serum Albumin; D004077:Digoxin