The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum.
A cDNA clone encoding a human cellular thyroid hormone binding protein (p55) has been isolated. The cDNA sequence contains a single open reading frame of 1524 nucleotides which encodes a polypeptide of 491 amino acids and a signal sequence of 17 amino acids. Two partial protein sequences determined from the purified protein correspond to the deduced amino acid sequence. In vitro translation of mRNA prepared by transcription of the cDNA clone yielded a protein of the size of an unprocessed precursor (Mr 57,000) as well as smaller fragments which were immunoprecipitated by antibodies against p55. The isolation of the cDNA clone should allow elucidation of the cellular function of this thyroid hormone binding protein which is present in the endoplasmic reticulum and nuclear envelope.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003488:Cyanogen Bromide; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D010446:Peptide Fragments; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011988:Receptors, Thyroid Hormone / Q000235:genetics*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D010446:Peptide Fragments; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D004247:DNA; D003488:Cyanogen Bromide