Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms.
A crude soluble extract (AWH) obtained from homogenized adult Nematospiroides dubius worms was fractionated into normal (NMIgAg) and immune (IMIgAg) antigens by sequential passage through Sepharose 4B, and CNBr-activated Sepharose 4B to which immunoglobulins from normal (NMIg) and N. dubius-infected mouse serum (IMIg) had been coupled. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions showed that MNIgAg and IMIgAg contained several proteins with molecular weights (MW) between 20,000 and 200,000. IMIgAg contained 2 polypeptides, MW 24,000 and 55,000, which reacted strongly in western blot analysis against IMIg; the larger molecule related to a cuticular component of the worm. Protective high MW AWH G-200 filtrates shared 5 polypeptides with IMIgAg (MW 85,000-200,000) together with several smaller molecules (MW 30,000-less than 10,000). The MW 55,000 and 24,000 components concentrated in IMIgAg were restricted to non-protective low MW AWH gel filtrates. BCF1 mice vaccinated with IMIgAg were protected against N. dubius. Worm numbers, size and fecundity were reduced to an extent commensurate with that obtained in mice vaccinated with AWH. Mice vaccinated with NMIgAg showed partial resistance. The role of surface and dominant parasite immunogens in immunity is discussed.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D006369:Heligmosomatoidea / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D009349:Nematode Infections / Q000517:prevention & control*; D009351:Nematospiroides dubius / Q000276:immunology*; D010270:Parasite Egg Count; D014611:Vaccination
D000906:Antibodies; D000947:Antigens, Helminth