[Relationship of fetal hind limb and fetal liver on amino acid metabolism in pregnant rats].
A tracer technique for injections into rat fetal abdomen in utero was employed to obtain a better knowledge of amino acid metabolism in fetal hind limb and fetal liver. Calculation of tissue fluid distribution via 3H-inulin space made possible an estimation of the fetal hind limb and fetal liver intracellular amino acid concentration based upon the fetal plasma and fetal tissue amino acid concentration. A significant concentration gradient between fetal plasma and fetal hind limb was found for glutamate (37.3) but not for alanine (9.7) or leucine (3.0). The radioactivity of fetal hind limb and fetal liver after the injection of 1 microCi of radioactive 14C-glutamate 14C-alanine or 14C-leucine into fetal abdomen was measured. In fetal hind limb, significant radioactivity was recognized after 14C-alanine administration, but not after 14C-glutamate administration as against the significant concentration gradient. However, in fetal liver, significant radioactivity was recognized after 14C-glutamate administration, but not after 14C-alanine administration. We concluded that in fetus, glutamate was released from the hind limbs and a large amount taken up by fetal liver.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D005314:Embryonic and Fetal Development; D005121:Extremities / Q000196:embryology* / Q000378:metabolism; D005260:Female; D005971:Glutamates / Q000378:metabolism; D006614:Hindlimb; D008099:Liver / Q000196:embryology* / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D005971:Glutamates