The dinitrophenyl group as a selective label in high-performance liquid chromatography of peptides.
1-Fluoro-2,4-dinitrobenzene can be used to selectively label histidine, tyrosine, and cysteine residues in maleylated proteins. The usefulness of the resulting chromophores for peptide mapping by high-performance liquid chromatography was demonstrated with the lectin from sainfoin (Onobrychis viciifolia). The 2,4-dinitrophenyl (Dnp) label also can be used in a hydrophobic modulation approach as the mobility of a labeled model peptide changes considerably when its Dnp group is removed by thiolysis. Application of the method for checking sequences obtained by DNA or amino acid methods was shown by experiments with Viciae lectins. The probable cleavage site that generates the pea lectin's beta-chain from the alpha-beta precursor was identified and the sequence differences between the lentil and pea lectin beta-chains were examined.
D002851:Chromatography, High Pressure Liquid; D004140:Dinitrophenols; D007202:Indicators and Reagents; D037102:Lectins; D010449:Peptide Mapping; D013056:Spectrophotometry, Ultraviolet
D004140:Dinitrophenols; D007202:Indicators and Reagents; D037102:Lectins