title: Ethidium bromide: destruction and decontamination of solutions.
abstract: Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution may be completely degraded by reaction with sodium nitrite and hypophosphorous acid. Only non-mutagenic reaction mixtures were produced. Destruction was greater than 99.8% in all cases; the limit of detection was 0.5 micrograms ethidium bromide per milliliter of solution. Ethidium bromide also may be removed completely from the above solutions by using Amberlite XAD-16 resin. The limit of detection was 0.05 micrograms ethidium bromide per milliliter of solution (0.27 micrograms/ml when cesium chloride solution was used).
mesh_terms: D003666:Decontamination / Q000379:methods*; D004996:Ethidium / Q000633:toxicity; D009152:Mutagenicity Tests; D012722:Sewage; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014865:Waste Disposal, Fluid
chemical_list: D012722:Sewage; D012996:Solutions; D004996:Ethidium