[Isolation of inosine-5'-monophosphate from fish muscles].
Conditions for transformation of tissue adenosine-5'-monophosphate (AMP) into inosine-5'-monophosphate (IMP) with the aid of endogenic AMP-aminohydrolase are developed resting on the studied properties of AMP-aminohydrolase (EC 3.5.4.6) from saltwater fish muscles (one of the enzymes participating in the nucleotide metabolism). Sorption of the nucleotide is performed on the activated charcoals A gamma-3 A gamma-5 which eluate IMP from acid solutions. It reduces the process of isolation, permits application of the acid wash solutions to remove salts; the alkaline ethyl alcohol-aid elution at the subsequent stages accelerates the process of nucleotide concentration by means of vacuum evaporation. The suggested approaches allow developing a simple method of IMP production from fish tissues which diminishes the cost of preparation.
D000659:AMP Deaminase / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D005399:Fishes / Q000378:metabolism*; D006868:Hydrolysis; D007291:Inosine Monophosphate / Q000302:isolation & purification* / Q000378:metabolism; D007292:Inosine Nucleotides / Q000302:isolation & purification*; D009132:Muscles / Q000032:analysis* / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet
D007292:Inosine Nucleotides; D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D000659:AMP Deaminase
Poluchenie inozin-5'-monofosfata iz myshts ryb.