Chromatin structure of the HLA-DR alpha gene in different functional states of major histocompatibility complex class II gene expression.
We utilized DNase I hypersensitivity mapping to study chromatin structure within the HLA-DR alpha gene. We found a single DNase I-hypersensitive site coinciding with the HLA-DR alpha gene promoter in all cells studied. Moreover, in cells that constitutively express HLA-DR, two additional DNase I-hypersensitive sites were observed. These lie within the first intron of the HLA-DR alpha gene and encompass DNA sequences that share homologies with regulatory loci of the immunoglobulin and immune response genes, as well as with core enhancer consensus sequences.
D001483:Base Sequence; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure*; D002874:Chromosome Mapping; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D005796:Genes; D006681:HLA-D Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D014158:Transcription, Genetic
D002843:Chromatin; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D003850:Deoxyribonuclease I