Suppressive effect of antibiotics on colony formation from human megakaryocyte progenitors (CFU-M) and granulocyte-macrophage progenitors (CFU-GM).
beta-Lactam antibiotics, moxalactam (LMOX), cefotaxime (CTX), flomoxef (FMOX), cefamandole (CMD), carbenicillin (CBPC) and sulbenicillin (SBPC), suppressed colony formation from human megakaryocyte progenitors (CFU-M) and granulocyte-macrophage progenitors (CFU-GM) dose-dependently. The suppressive potencies for both progenitors were weakest for CBPC and SBPC, moderate for LMOX and FMOX, and strongest for CMD and CTX. The stainability of glycoprotein IIb/IIIa complex in the megakaryocyte colonies by the monoclonal antibody was decreased by LMOX and CTX. These data suggest that beta-lactam antibiotics directly suppress proliferation of CFU-M and CFU-GM.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006651:Histocytochemistry; D006801:Humans; D066298:In Vitro Techniques; D007769:Lactams; D008533:Megakaryocytes / Q000032:analysis / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000032:analysis
D000900:Anti-Bacterial Agents; D007769:Lactams; D010980:Platelet Membrane Glycoproteins