title: Cellular inflammatory responses in human allergic skin reactions.
abstract: To define better the role of inflammation in the response to pollen antigens, we have used our skin chamber model to study inflammatory cells recovered from the sites of ongoing allergic reactions. In 15 atopic subjects, paired skin blister sites were simultaneously challenged with ragweed- or grass-pollen antigen or buffer for 5 hours. There were 10 times as many cells recovered at antigen (20.7 X 10(5)) than at buffer (2.0 X 10(5)) sites, p less than 0.005; greater than 97% of the cells recovered were neutrophils. The number of cells recovered at the antigen sites correlated with the total amount of histamine released (r = 0.57; p less than 0.05) but not with the extinction dilution skin test reactivity nor with the intensity of the late cutaneous allergic response measured 6 hours after the injection of antigen. Phase-contrast microscopic examination of the cells recovered from the antigen sites demonstrated that 82% to 95% were polarized compared to 0% to 1.5% of autologous blood neutrophils obtained simultaneously from the peripheral blood. Antigen site cells were as capable of serum-dependent phagocytosis as peripheral blood neutrophils. There was no significant difference in the migratory response to buffer, the chemoattractant N-formyl-methionyl-leucyl-phenylalanine, or leukotriene B4, but there was a significantly decreased response to platelet-activating factor when the cells recovered from antigen sites were compared to autologous blood neutrophils.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000941:Antigens / Q000009:adverse effects; D002634:Chemotaxis, Leukocyte; D005122:Exudates and Transudates / Q000473:pathology; D005260:Female; D006632:Histamine / Q000032:analysis; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000473:pathology*; D007249:Inflammation; D008297:Male; D009504:Neutrophils / Q000473:pathology* / Q000502:physiology; D010587:Phagocytosis; D011058:Pollen / Q000009:adverse effects; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000276:immunology / Q000473:pathology*
chemical_list: D000941:Antigens; D006632:Histamine