[Quantitative evaluation of the uptake capacity of intact thrombocytes using fluorescent dyes].
A new approach to quantitative determination of fluorescent dye uptake by intact cells is suggested. Fluorescent amine acridine orange selectively accumulating in 5HT granules of platelets has been used. Fluorescence signal analysis allows the estimation of a relative granule volume and the ratios of acridine orange transfer over cytoplasmic and granule membranes. The following results were obtained in human and rabbit platelets: a relative granule size was 14 +/- 1 % and 29 +/- 2 % of the total cell volume, intra-granule to extra-granule dye concentration ratios were 2260 +/- 382 and 30000 +/- 5550, while intra-cytoplasm to extra-cytoplasm concentration ratios were 375 +/- 60 and 225 +/- 60, respectively.
D000165:Acridine Orange; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D011817:Rabbits; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D000165:Acridine Orange
Kolichestvennaia otsenka akkumuliruiushcheĭ sposobnosti intaktnykh trombotsitov s pomoshch'iu fliuorestsentnykh krasiteleĭ.