title: Ampicillin-dextrin agar medium for the enumeration of Aeromonas species in water by membrane filtration.
abstract: Published selective media were evaluated for the isolation of Aeromonas spp. from environmental samples by membrane filtration. Satisfactory recoveries were obtained only with mA agar (Rippey & Cabelli) and dextrin-fuchsin-sulphite agar (Schubert), but neither was sufficiently selective. The positive aspects of these two media were combined in a new medium, ampicillin-dextrin agar. Recovery from pure cultures and environmental samples was optimal at an ampicillin concentration of 10 mg/l and incubation for 24 h at 30 degrees C under aerobic conditions, and specificity was high (i.e. confirmation rate usually greater than 90%, no false negative colonies encountered). The medium can also be used for isolation of Aeromonas spp. from sea water provided that the vibriostatic agent 0/129 is added at 50 mg/l.
mesh_terms: D000333:Aeromonas / Q000254:growth & development / Q000302:isolation & purification*; D000667:Ampicillin; D003470:Culture Media; D003912:Dextrins; D005374:Filtration; D005618:Fresh Water; D012623:Seawater; D012722:Sewage; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D003912:Dextrins; D012722:Sewage; D000667:Ampicillin