ADH and phorbol ester increase immunolabeling of the toad bladder apical membrane by antibodies made to granules.
Polyclonal antibodies were raised to isolated toad bladder granules. On immunoblots, the anti-granule antiserum specifically stained components of isolated granules. Immunocytochemically, the anti-granule antiserum labeled the apical surface of the bladder. Immunolabeling increased at the apical surface when the bladder was exposed to antidiuretic hormone (ADH) serosally or phorbol ester (PMA) mucosally--conditions which stimulate apical granule exocytosis. The increase in granule epitopes on the apical surface was sixfold greater than the net increase in surface area.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011898:Ranidae; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D001743:Urinary Bladder / Q000648:ultrastructure*; D014667:Vasopressins / Q000494:pharmacology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D014667:Vasopressins; D013755:Tetradecanoylphorbol Acetate