The labelling of peptides and proteins with a new fluorophore: N-acetyl-DL-(p-N',N'-dimethylamino)phenylalanine.
It was found that N-acetyl-DL-(p-N',N'-dimethylamino)phenylalanine, in the form of azlactone, can be introduced into a peptide or protein molecule as a new convenient fluorescent marker. A fluorophore of similar properties: N-acetyl-(p-amino)phenylalanine can be introduced into a peptide chain by the reaction with the azlactone of N-acetyl-(p-nitro)phenylalanine followed by reduction of nitro group to amino group. This method, however, cannot be applied to proteins.
D005456:Fluorescent Dyes / Q000138:chemical synthesis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009113:Muramidase / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; C052922:N-acetyl-4-(N',N'-dimethylamino)phenylalanine azlactone; D010455:Peptides; D011506:Proteins; D010649:Phenylalanine; D009113:Muramidase