Enzymatic inactivation of aztreonam by faecal enzyme preparations from healthy volunteers.
The enzymatic inactivation of aztreonam by the faecal flora was investigated in 25 healthy human volunteers. Ten volunteers received aztreonam orally and 15 volunteers did not receive antibiotic treatment. Residual aztreonam and its inactivated open ring form were simultaneously detected by an HPLC-assay. Independent of aztreonam treatment, beta-lactamase activity was detected in the faecal flora of 20 volunteers. The inactivation of aztreonam by the faecal supernatants was inhibited by clavulanic acid.
D000328:Adult; D001398:Aztreonam / Q000378:metabolism*; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000201:enzymology*; D006801:Humans
D001398:Aztreonam