Improved enzyme-linked immunosorbent assay for determination of soy protein in meat products.
An indirect, competitive enzyme-linked immunosorbent assay (ELISA) has been developed for quantitation of soy protein in meat products. The methodology allows rapid aqueous extraction of meat samples into a liquid form suitable for assay. The assay is highly specific for soy protein and is designed to measure soy protein levels between 1 and 10% of the wet weight of the sample. Standardized, stabilized reagents for carrying out the procedure are commercially available in a kit. The analysis, including sample preparation, can be completed within a workday, and the actual immunoassay in less than 60 min.
D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D014674:Plant Proteins, Dietary / Q000032:analysis*; D030262:Soybean Proteins; D013552:Swine
D007202:Indicators and Reagents; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins