High-performance liquid chromatographic method for the analysis of N tau-methylhistidine in food, chicken excreta, and rat urine.
A reliable high-performance liquid chromatographic method that utilizes a reversed-phase separation with ion-pairing and post-column fluorescence derivatization for the analysis of N tau-methylhistidine in food, chicken excreta, and rat urine is described. N tau-Methylhistidine in the hydrolyzed sample is first roughly separated from acid and neutral amino acids by an ion-exchange column. The N tau-methylhistidine fraction is then evaporated and the residue is dissolved in the mobile phase (15 mM sodium octane sulfonate in 20 mM potassium phosphate), and subjected to high-performance liquid chromatography.
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000032:analysis*; D005504:Food Analysis; D006639:Histidine / Q000031:analogs & derivatives*; D008762:Methylhistidines / Q000032:analysis* / Q000652:urine; D051381:Rats
D008762:Methylhistidines; D006639:Histidine; C028118:3-methylhistidine