[Functional state of the hepatocyte cytoplasmic network in rats with thermal burns].
Intensity of xenobiotic biotransformation estimated by means of "hexenal" test as well as enzymatic activity in liver microsomes were studied in rats with burns corresponding to impairment of 3%, 10% and 20% of body surface within 1 hr, 1, 3, 6 and 15 days. Soporific effect of hexenal was distinctly increased in the burns, which correlated to the severity of thermic impairment. Activities of amidopyrine-N-demethylase and aniline hydroxylase as well as content of protein, cytochromes P450 and b5 were decreased in liver microsomes. At the same time, lipid peroxidation was activated in microsomes, content of diene conjugates and diene ketones was increased in blood serum and in liver postmitochondrial fraction of experimental animals. Disfunction of hepatocyte cytoplasmic structures appears to be among the factors responsible for a body intoxication in burns.
D000633:Aminopyrine N-Demethylase / Q000037:antagonists & inhibitors; D000815:Aniline Hydroxylase / Q000037:antagonists & inhibitors; D000818:Animals; D002056:Burns / Q000201:enzymology* / Q000378:metabolism / Q000503:physiopathology; D065607:Cytochrome P-450 Enzyme Inhibitors; D003573:Cytochrome b Group / Q000037:antagonists & inhibitors; D015786:Cytochromes b5; D006591:Hexobarbital; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology* / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D012890:Sleep / Q000187:drug effects
D065607:Cytochrome P-450 Enzyme Inhibitors; D003573:Cytochrome b Group; D008054:Lipid Peroxides; D015786:Cytochromes b5; D006591:Hexobarbital; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase
Funktsional'noe sostoianie tsitoplazmaticheskoĭ seti gepatotsitov u krys s termicheskim ozhogom.