Structures of novel sialylated O-linked oligosaccharides isolated from human erythrocyte glycophorins.
The O-linked oligosaccharides attached to human erythrocyte glycophorins were extensively characterized. In addition to the previously described disialylated tetrasaccharide, NeuNAc alpha 2----3Gal beta 1----3 (Neu-NAc alpha 2----6)GalNAcOH and monosialylated trisaccharide, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, novel trisialylated oligosaccharides were isolated. Methylation analysis, fast atom bombardment-mass spectrometry, and enzymatic degradation were used to elucidate the following novel structures: formula; see text: These results suggest that O-linked oligosaccharides with a disialosyl group, NeuNAc alpha 2----8NeuNAc alpha 2----, may be present in various tissues.
D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006021:Glycophorins / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis*
D006021:Glycophorins; D009844:Oligosaccharides; D012794:Sialic Acids; D012795:Sialoglycoproteins