5'-adenosine monophosphate inhibits ternary complex formation by rat liver eIF-2.
The initiation factor 2 (eIF-2) was purified from rat liver essentially to homogeneity. The effect of physiological concentrations of AMP on ternary complex formation by eIF-2 was tested. It is shown that AMP inhibits ternary complex formation (ca. 50% at 100 microM AMP). None of the other adenine-nucleotides tested (ADP, ATP, cAMP, NADH, NAD+ and NADPH) affected ternary complex formation.
D000227:Adenine Nucleotides / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D015852:Eukaryotic Initiation Factor-2; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D046911:Macromolecular Substances; D010448:Peptide Initiation Factors / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats
D000227:Adenine Nucleotides; D015852:Eukaryotic Initiation Factor-2; D046911:Macromolecular Substances; D010448:Peptide Initiation Factors; D011506:Proteins; D000249:Adenosine Monophosphate; D012343:RNA, Transfer