Increased erythrocyte stearic acid desaturation in rats with chemically induced colorectal carcinomas.
Our previous studies have demonstrated a desaturation of stearic acid related to oleic acid in the lipid layer of erythrocytes in patients with malignancies. This study investigated the stearic acid desaturation in red blood cell membranes of rats during the induction of colorectal tumours. Male Sprague-Dawley rats were injected weekly with dimethylhydrazine (DMH) and sacrificed at 4-week intervals. Blood was withdrawn via heart puncture, collected in EDTA bottle and erythrocytes separated by centrifugation. Total lipid extraction was carried out and analysed with gas liquid chromatography. In the control rats (injected with normal saline) the mean of the stearic to oleic acid ratio in erythrocyte membranes was 2.0 +/- 0.3 (n = 28, range 1.51-2.62) compared to a mean of 0.94 +/- 0.16 (n = 0.5-1.23) in tumour bearing rats (p less than 0.001). The increased desaturation occurred in parallel with appearance of tumours. These data suggest the regulation of stearic acid desaturation is an important adaptive mechanism of membrane fluidity and could be a useful chemical marker for malignancy.
D000818:Animals; D003110:Colonic Neoplasms / Q000097:blood / Q000139:chemically induced*; D004127:Dimethylhydrazines; D004910:Erythrocyte Membrane / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity; D019301:Oleic Acid; D009829:Oleic Acids / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D012004:Rectal Neoplasms / Q000097:blood / Q000139:chemically induced*; D013229:Stearic Acids / Q000097:blood*
D004127:Dimethylhydrazines; D009829:Oleic Acids; D013229:Stearic Acids; D019301:Oleic Acid; C031183:stearic acid