Reversible activation/inactivation of the deacylation/acylation cycle in rat liver microsomes.
Rat liver microsomes incorporate [14C]palmitoyl CoA into membrane phospholipids via the deacylation/acylation cycle. This activity is reversibly inactivated/activated by treatment of the microsomes with ATP, MgCl2, and 105,000 g supernatant or with 105,000 g supernatant alone. These observations suggest that the acylation cycle is controlled by a mechanism involving phosphorylation/dephosphorylation. As the pool of lysolecithin in the membranes is not altered by conditions increasing incorporation of palmitoyl CoA into phospholipid, it is probable that the site of regulation of deacylation/acylation is at the acyltransferase rather than the phospholipase.
D000215:Acylation; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D008563:Membrane Lipids; D010743:Phospholipids; D015636:Magnesium Chloride; D010171:Palmitoyl Coenzyme A; D000255:Adenosine Triphosphate; D008274:Magnesium