Effect of phagocyte membrane stimulation on antibiotic uptake and intracellular bactericidal activity.
Phagocytosis stimulated a substantial increase in clindamycin uptake and a smaller increase in rifampin accumulation by polymorphonuclear leukocytes. The effect of this increased antibiotic uptake on intraphagocytic bactericidal activity was evaluated. Although zymosan stimulated antibiotic uptake by polymorphonuclear leukocytes, neither zymosan nor formyl-methionyl-leucyl-phenylalanine enhanced the ability of clindamycin or rifampin to kill ingested staphylococci. Properties other than antibiotic uptake are important in determining intraphagocytic bactericidal activity.
D001433:Bacteriolysis / Q000187:drug effects; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002981:Clindamycin / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D007424:Intracellular Fluid / Q000032:analysis; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects; D012293:Rifampin / Q000378:metabolism* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D015054:Zymosan / Q000494:pharmacology
D002981:Clindamycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D012293:Rifampin