Estrogen stimulation of phosphatidylinositol metabolism in mouse uterine tissue.
The effect of estrogens on incorporation of labeled precursor into inositol-containing phospholipids (PI) from ovariectomized mouse uterus was investigated. The results indicate that diethylstilbestrol (DES) a potent mitogen, stimulated incorporation of myo-[3H]inositol into uterine PI in 1-3 h, with maximal incorporation occurring at 6 h. This activity followed a dose-dependent increase, with a maximal effect at 5 micrograms/kg. Incorporation of radiolabeled phosphorous into the polyphosphoinositides was also increased in estrogen-stimulated uterine tissue. Studies using a weak uterotropic DES derivative, Z,Z-dienestrol, produced an early stimulation of the PI response comparable to DES. This activity was increased by Z,Z-dienestrol, with minimal estrogen receptor occupancy, and did not result in stimulation of DNA synthesis. These findings would suggest that uterine PI stimulation may not occur via an estrogen receptor-mediated mechanism related to tissue proliferation induced by estrogens.
D000818:Animals; D004028:Dienestrol / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D007294:Inositol / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D010052:Ovariectomy; D010710:Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D011800:Quinestrol / Q000031:analogs & derivatives / Q000494:pharmacology; D011960:Receptors, Estrogen / Q000502:physiology; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D004967:Estrogens; D010710:Phosphates; D010716:Phosphatidylinositols; D011960:Receptors, Estrogen; D007294:Inositol; D004054:Diethylstilbestrol; C010722:nylestriol; D011800:Quinestrol; D004028:Dienestrol