Effect of gizzerosine on acid secretion by isolated mucosal cells of chicken proventriculus.
Mucosal cells of the chicken proventriculus were isolated by a collagenase perfusion method and O2 uptake by the isolated cells was measured as an index of the activity of gastric acid secretion. Oxygen consumption was enhanced by histamine; this effect was augmented by the coexistence of isobutylmethylxanthine, an inhibitor of cyclic adenosine 5.-monophosphate (AMP) phosphodiesterase, and suppressed by imidazole, an activator of the enzyme. The action of histamine was inhibited by cimetidine, an antagonist of the histamine H2 receptor. These results indicate that the isolated cells retained the capacity to take up O2, responding to histamine via the H2 receptor and probably the cyclic AMP level. Gizzerosine (2-amino-9-(4-imidazolyl)-7-azanonanoic acid) also stimulated O2 consumption by the isolated cells. The effect of gizzerosine was cancelled by cimetidine, suggesting that the mechanism by which gizzerosine acts on the mucosal cells is similar to that of histamine action. These observations are consistent with our previous presumption that gizzerosine causes gizzard erosion by enhancing gastric acid secretion in chickens.
D000818:Animals; D002645:Chickens / Q000502:physiology*; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology*; D007093:Imidazoles / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011531:Proventriculus / Q000187:drug effects* / Q000378:metabolism
D007093:Imidazoles; C045114:gizzerosine