Transcription of H-2 and Qa genes in embryonic and adult mice.
We have isolated cDNAs for three class I genes from an 8.5-day C57Bl/6 mouse embryo cDNA library. Two of these cDNAs encode the classical transplantation antigens, H-2Kb and H-2Db. The third is a novelly spliced form of a Qa region gene, Q9, which lacks several exons including that encoding the transmembrane domain. Since this mRNA is detected on membrane-bound polysomes it may encode a secreted protein. Transcripts for these three genes and the pseudo-allele of Q9, Q7, were present at very low levels in the 8.5-day and 14.5-day embryo and in the 2-day neonate, compared to most adult tissues. Q10, the liver-specific secreted class I gene product in adults, is preferentially expressed in yolk sac and in fetal liver. We found no embryo-specific class I gene expression.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004622:Embryo, Mammalian; D005091:Exons; D005796:Genes; D006183:H-2 Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I; D007438:Introns; D007739:L Cells / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D014018:Tissue Distribution; D014158:Transcription, Genetic
D000954:Antigens, Surface; D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I; C032907:Q surface antigens; D004247:DNA