Detection of interference by cyclobenzaprine in liquid-chromatographic assays of tricyclic antidepressants.
We evaluated a technique for detecting cyclobenzaprine interference with liquid-chromatographic assays for tricyclic antidepressants. The technique involves dual-wavelength absorbance monitoring of the column effluent at 214 and 254 nm. Ratios of analyte peak heights at each wavelength are used to check for the presence of co-eluting interferences. With this technique, one can detect interference with an amitriptyline assay caused by 10 micrograms of cyclobenzaprine per liter.
D000639:Amitriptyline / Q000031:analogs & derivatives* / Q000494:pharmacology; D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D002853:Chromatography, Liquid; D006801:Humans; D009125:Muscle Relaxants, Central / Q000494:pharmacology*; D013053:Spectrophotometry
D000929:Antidepressive Agents, Tricyclic; D009125:Muscle Relaxants, Central; D000639:Amitriptyline; C004704:cyclobenzaprine