Phospholipase C and the physical states of polar head groups of lipids.
Activity of phospholipase C from Clostridium perfringens on liposomes made from sn-3-phosphatidylcholine, dimyristoyl (DMPC), dipalmitoyl (DPPC) or distearoyl (DSPC) was measured at various temperatures and was correlated with their gel/liquid-crystalline phase transitions (Tc:23, 41.5, 52 degrees C for DMPC, DPPC, DSPC, respectively). In all cases, the activity of phospholipase C was high in the gel phases of the substrates and was almost zero in their liquid-crystalline phases. Fluorescence depolarization measurements of N-dansyl-sn-3-phosphatidylethanolamine (DPE) and 1,6-diphenyl-1,3,5-hexatriene (DPH) incorporated into the liposomes showed that both the head group and the alkyl chains of the lipids were immobilized in the gel phases but were highly mobile in the liquid-crystalline phase. These results indicate that the rotational mobility of lipids (both of the head groups and the alkyl chains) was not a major factor in the phospholipase C reaction. It is inferred that some electrostatic and/or hydrophobic interactions might play important roles in regulation of the phospholipase C activity.
D003619:Dansyl Compounds; D004161:Diphenylhexatriene; D004560:Electricity; D008051:Lipid Bilayers; D008081:Liposomes / Q000378:metabolism*; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D012399:Rotation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D010738:Type C Phospholipases / Q000378:metabolism*
D003619:Dansyl Compounds; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D004161:Diphenylhexatriene; C024284:dansyl phosphatidylethanolamine; D010738:Type C Phospholipases