Location of penicilloyl groups on CNBr fragments of the albumin from penicillin-treated patients.
Two fixation sites for penicilloyl groups on human albumin were demonstrated. Using CNBr cleavage the first site was located between methionine 123 and methionine 297 and the second one between methionine 297 and the C-terminal residue. In both cases, penicilloyl groups were unmasked by pronase degradation or disulfide bond reduction.
D001665:Binding Sites; D003488:Cyanogen Bromide / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D010406:Penicillins / Q000097:blood*; D011402:Pronase / Q000494:pharmacology; D012709:Serum Albumin / Q000378:metabolism*
D010406:Penicillins; D012709:Serum Albumin; D011402:Pronase; D003488:Cyanogen Bromide