High performance liquid chromatography determination of cystathionase activity in human and rabbit lenses.
Cystathionase activity has been demonstrated in human and rabbit lenses, using L-homoserine as substrate. The amount of activity found was quite low, 0.05 and 0.08 nmol of alpha-ketobutyrate formed/h/mg protein in human and rabbit lenses, respectively. The human lens cystathionase was found to have a pH maximum of 8.5 and a Km of 23.8 mM for L-homoserine. A sensitive assay, involving the use of high performance liquid chromatography, was developed to measure the small amounts of alpha-ketobutyrate produced.
D000818:Animals; D002087:Butyrates / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003540:Cystathionine / Q000494:pharmacology; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D006714:Homoserine / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007908:Lens, Crystalline / Q000201:enzymology*; D008190:Lyases / Q000378:metabolism*; D011817:Rabbits
D002087:Butyrates; D003540:Cystathionine; C005087:alpha-ketobutyric acid; D006714:Homoserine; D008190:Lyases; D003542:Cystathionine gamma-Lyase