title: Isolation and properties of androgenic gland hormone from the terrestrial isopod, Armadillidium vulgare.
abstract: The androgenic gland hormone (AGH) was purified from the male reproductive organs, including the androgenic glands, of Armadillidium vulgare by pH adjustment, ammonium sulfate precipitation, gel filtration, ion-exchange chromatography, and three kinds of high-performance liquid chromatography (HPLC). AGH consisted of two molecular forms, AGH I and AGH II. They were judged as homogeneous by HPLC, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and isoelectrofocusing, though their N-terminal residues were not detected. They seem to be monomeric proteins consisting of 157 and 166 amino acid residues, having molecular weights of 17,000 +/- 800 and 18,300 +/- 1000, and having isoelectric points of about 4.5 and 4.3, respectively. The activities of AGH I and AGH II were almost the same and their respective specific activities were 560 and 430 times that of the crude extract.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003445:Crustacea / Q000502:physiology*; D004702:Endocrine Glands / Q000502:physiology*; D005837:Genitalia, Male / Q000502:physiology*; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones / Q000302:isolation & purification*; D008297:Male; D008970:Molecular Weight; D004490:Xenarthra / Q000502:physiology*
chemical_list: D000596:Amino Acids; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones; C053644:androgenic gland hormone (Armadillidium)