Repetitive hit-and-run fluoroimmunoassay for T-2 toxin.
A monoclonal antibody for T-2 toxin is converted to a Fab'-fluorescein derivative. The latter is specifically complexed onto a T-2 agarose gel. Fifteen successive doses of T-2 ranging from 1 to 50 ng are then repetitively and linearly detected using a column packed with a small volume (0.2 ml) of this gel without recharging with Fab'-fluorescein. For these assays the effluent from the column is monitored with a spectrofluorometer.
D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid / Q000379:methods; D005452:Fluoresceins; D005453:Fluorescence; D007118:Immunoassay / Q000379:methods; D007140:Immunoglobulin Fab Fragments; D007700:Kinetics; D012717:Sesquiterpenes / Q000032:analysis*; D013605:T-2 Toxin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D007140:Immunoglobulin Fab Fragments; D012717:Sesquiterpenes; D013605:T-2 Toxin