Intravascular metabolism of lipoprotein cholesteryl esters in African green monkeys: differential fate of doubly labeled cholesteryl oleate.
High density lipoproteins (HDL), doubly labeled with [3H]cholesteryl oleate and cholesteryl [14C]oleate, were reinjected to study HDL cholesteryl ester metabolism in African green monkeys. The transfer of labeled HDL cholesteryl ester to low density lipoprotein (LDL) was rapid and equilibration of the [3H]cholesteryl oleate and cholesteryl [14C]oleate specific activities in LDL and HDL occurred within 90 min after reinjection. The apparent rates of disappearance from the circulation of the two moieties of the cholesteryl ester were different. In the same four animals, the residence time for the turnover of plasma [3H]cholesterol averaged 6.1 days while the residence time for the removal of cholesteryl [14C]oleate from plasma was approximately 2.1 days. These results suggest that for some lipoprotein cholesteryl esters removed from plasma, the cholesterol moiety subsequently reappeared in plasma. The difference between the rate of decay of the 14C-labeled fatty acid moiety, which represents all of the cholesteryl ester removed from plasma (0.48 pools/day) and the decay of the 3H-labeled cholesterol moiety, which represents the sum of cholesteryl ester removal and cholesterol reappearance (0.16 pools/day), is the fraction of the cholesteryl ester pool recycled per day (0.32 pools/day or 22.5 mg/kg per day). In other words, approximately 68% of the cholesterol moiety that was removed from plasma as cholesteryl oleate reappeared in the plasma cholesterol pool. These studies support the concept that an efficient reutilization cycle for plasma cholesterol occurs, i.e., the cholesteryl ester molecule can exit and the cholesterol moiety can re-enter plasma without effective equilibration of the cholesterol moiety with extravascular cholesterol pools.
D000818:Animals; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008074:Lipoproteins / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male
D002788:Cholesterol Esters; D008074:Lipoproteins; C014409:cholesteryl oleate; D002784:Cholesterol