[Morphometric analysis of the toxic effect of ethanol on the structure of chromatin in interphase nuclei of rat hepatocytes].
A method of automated morphometric analysis of hepatic cell interphase nuclear chromatin (CHIN) structure was elaborated using scanning microspectrophotometer SMP-05 ("Opton", FRG). Liver sections of rats were used to investigate hepatic cell CHIN structure. The animals were administered ethanol intraperitoneally once per day within a month period in doses 4, 6, 8, 10 g/kg (1/3-4/5 LD50). Morphometric indexes indicating dose-dependent changes in hepatic cell CHIN structure are demonstrated. Statistical analysis of morphometric data has led to the conclusion that hepatic cell CHIN structural modification depends on two factors: ethanol damaging effect and hepatic cell regeneration.
D000435:Alcoholic Intoxication / Q000473:pathology*; D000818:Animals; D002843:Chromatin / Q000187:drug effects* / Q000648:ultrastructure; D007399:Interphase; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats
D002843:Chromatin
Morfometricheskiĭ analiz toksicheskogo deĭstviia étanola na struktury khromatina interfaznykh iader gepatsitov krys.