title: Capnocytophaga species: increased resistance of clinical isolates to serum bactericidal action.
abstract: Capnocytophaga, a newly recognized genus of capnophilic gram-negative bacilli, is part of the normal oral flora. The capacity of Capnocytophaga to cause sepsis and local infections in both immunocompromised and nonimmunocompromised hosts has been documented. Given the recognition that serum resistance may contribute to the virulence of some gram-negative bacteria, we attempted to define the serum sensitivity of clinical isolates of Capnocytophaga from blood and other sites of infection. Whereas nine of nine isolates from human subgingival plaque showed greater than 95% loss of viability under standardized assay conditions, nonoral isolates exhibited variable serum sensitivity. Six of six isolates from blood showed considerable serum resistance (mean survival, 59.7% +/- 38.3%; range, 14.4%-113.3%). Comparison of the electrophoretic mobilities of lipopolysaccharides (LPSs) from sensitive and resistant strains revealed reduced LPS heterogeneity and lower apparent molecular weight among serum-resistant strains. Thus, serum resistance, possibly influenced by LPS structure, may be an important factor contributing to the pathogenic potential of Capnocytophaga spp.
mesh_terms: D001770:Blood Bactericidal Activity; D002206:Capnocytophaga / Q000032:analysis / Q000276:immunology*; D003167:Complement Activation; D041964:Cytophagaceae / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis
chemical_list: D008070:Lipopolysaccharides