Liver porphyrinogen carboxylase in hexachlorobenzene porphyric rats. Studies with intermediate porphyrinogens of series III and with uroporphyrinogen I.
The present work studies the action of hexachlorobenzene (HCB) on the decarboxylation of uroporphyrinogen (Urogen) I and III and also on the decarboxylation of intermediate porphyrinogens of series III under different conditions using liver of normal and porphyric rats as enzyme source. The same enzyme is involved in the Urogen decarboxylation of both isomeric series I and III and catalyses the four steps in both cases. HCB affects all of them. HCB blocks the four steps of Urogen III decarboxylation to the same degree, as a function of intoxication time. HCB leads, in general, to an increase in the efficiency (Km/Vmax) of the porphyric system. These data can be interpreted as a reaction of the organism to overcome the enzymatic blockade.
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002722:Chlorobenzenes; D005260:Female; D006581:Hexachlorobenzene; D008099:Liver / Q000201:enzymology*; D011164:Porphyrias / Q000139:chemically induced / Q000201:enzymology*; D011165:Porphyrinogens / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014575:Uroporphyrinogen Decarboxylase / Q000378:metabolism*; D014577:Uroporphyrinogens / Q000378:metabolism*
D002722:Chlorobenzenes; D011165:Porphyrinogens; D014577:Uroporphyrinogens; D006581:Hexachlorobenzene; D002262:Carboxy-Lyases; D014575:Uroporphyrinogen Decarboxylase