The H-2Kkml mutation: nucleotide sequence and comparative analysis.
Nucleotide sequence analysis of mRNA from the class I murine MHC mutant H-2Kkml has established a site of mutation to be at the codon for amino acid position 152. Complete sequence information for the nucleotides coding for amino acids 136-163 demonstrates an A----C alteration at the codon for amino acid 152, changing Asp (GAT) in Kk to Ala (GCT) in Kkml. Several other murine and human class I MHC variants have similar alterations at amino acid position 152, resulting in altered biological activity. Finally, the pH-2III pseudogene of the H-2k haplotype has a GCT codon at amino acid position 152, suggesting that the GCT codon occurring in Kkml is the result of a gene conversion event.
D000818:Animals; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D008808:Mice, Inbred CBA; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics*
D006183:H-2 Antigens; D012333:RNA, Messenger