[Stimulating effect of arginine vasopressin on the release of free fatty acids by isolated rat adipose cells].
Arginine vasopressin (AVP) action on the lipolysis, both the basic and induced with noradrenaline, was studied in adipocytes isolated from epididymal fat pads of Wistar rats sacrificed after 24-hr food deprivation. Adipocytes were exposed to hormones for 10 or 15 min. Lipolysis was estimated by measuring FFA release. AVP alone in relatively small concentrations (8 X 10(-9) M and 8 X 10(-11) M) stimulated FFA release by 39.6% and 56.3%, resp. Higher concentrations (8 X 10(-7) M) exerted no effect. Having been added to noradrenaline, however, AVP in concentrations 8 X 10(-7) M and 8 X 10(-9) M decreased lipolytic effect of the former, although the decrease was statistically insignificant. Whether the activating effect of AVP is due to the stimulation of lipolysis or to changes in membrane permeability for FFA, is to be elucidated.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001127:Arginine Vasopressin / Q000008:administration & dosage / Q000494:pharmacology*; D004347:Drug Interactions; D004822:Epididymis; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D066298:In Vitro Techniques; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D009638:Norepinephrine / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D005230:Fatty Acids, Nonesterified; D001127:Arginine Vasopressin; D009638:Norepinephrine
Stimuliruiushechee deĭstvie arginin-vazopressina na vydelenie svobodnykh zhirnykh kislot izolirovannymi zhirovymi kletkami krys.