Membrane polyunsaturated fatty acids and lithium-sodium countertransport in human erythrocytes.
Two groups of individuals, 26 normotensive normolipemic and 37 normotensive hyperlipemic, all without family history of hypertension have been selected in attempt to demonstrate whether Li-Na countertransport of erythrocytes is influenced by plasma and membrane lipid composition. The maximal rate of Li-Na countertransport was elevated in hyperlipemics (0.344 +/- 0.168 vs 0.220 +/- 0.074 mmol/l erythrocytes/h). This difference is highly significant. Hyperlipemics had different composition of membrane lipids than normals. The most important variations were: increase of palmitic, palmitoleic and total saturated fatty acids (SFA) as well as increase of cholesterol/phospholipids ratio (C/PL); in contrast, hyperlipemics had a reduced amount of linoleic acid and total unsaturated fatty acids (UFA) as well as total polyunsaturated fatty acids (PUFA). Consequently, UFA/SFA and PUFA/SFA ratios were lower than in normals. Li-Na countertransport was negatively correlated with the amount of PUFA (P less than 0.02), whereas it was positively correlated with the following parameters: oleic/linoleic ratio (p less than 0.02), monounsaturated fatty acids/polyunsaturated fatty acids ratio (p less than 0.03) as well as with the SFA + monounsaturated fatty acid/PUFA ratio (p less than 0.03). These findings suggest that the V max of Li-Na countertransport in erythrocytes is influenced by the lipid composition of the membrane.
D000328:Adult; D001692:Biological Transport; D001794:Blood Pressure; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D006801:Humans; D008055:Lipids / Q000097:blood; D008094:Lithium / Q000097:blood*; D008297:Male; D008563:Membrane Lipids / Q000097:blood; D008875:Middle Aged; D012964:Sodium / Q000097:blood*
D005231:Fatty Acids, Unsaturated; D008055:Lipids; D008563:Membrane Lipids; D008094:Lithium; D012964:Sodium