Isolation and characterization of a low molecular weight growth-promoting factor from human plasma.
A low molecular weight growth factor (LMW-GF) enriched preparation was purified from human plasma after ultrafiltration or gel filtration by means of molecular sieving chromatography low pressure reversed phase chromatography (LP-RPLC) and electrophoresis. Purification was monitored by a biological assay testing the capacity of the fractions to enhance the sulfation activity of the somatomedins/insulin-like growth factors on chick embryo cartilage. Analysis of its chemical nature show that it is hydrophilic, stable to heat, resistant to most of the proteases but that it is degraded by acid hydrolysis or carboxypeptidase Y action. UV absorption spectrum and ion-exchange chromatographic retention behavior support the hypothesis that the most purified active preparation includes a peptide structure. The presence of sugar is suggested by concanavalin A binding experiments. The fact that the purification fractions also enhance thymidine uptake by other cell lines (fibroblasts, activated lymphocytes) widens the role of such small plasma molecules in the field of growth factor activities.
D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism; D002642:Chick Embryo; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004261:DNA Replication / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006133:Growth Substances / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D013431:Sulfates / Q000378:metabolism; D014462:Ultrafiltration
D006133:Growth Substances; D013431:Sulfates