title: Normal murine melanocytes in culture.
abstract: A major obstacle to applying the techniques of molecular biology to the genetics and cell biology of pigmentation has been our inability to grow normal murine melanocytes in culture. We report here the establishment and characterization of continuously proliferating cultures of cutaneous pigment cells from seven strains of mice. Melanocytes were grown from the dermis of newborn mice in medium containing 12-0-tetradecanoyl-13-phorbol-acetate; a substance, such as melanotropin, that raises intracellular levels of cyclic AMP; and an extract made from human placenta.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004261:DNA Replication / Q000187:drug effects; D007621:Karyotyping; D007700:Kinetics; D008544:Melanocytes / Q000166:cytology*; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D013755:Tetradecanoylphorbol Acetate