A new agglutinin from the Tulipa gesneriana bulbs.
Two agglutinins with different agglutinating activity exist in Tulipa gesneriana bulbs. One is the T. gesneriana lectin which agglutinates yeasts as reported previously [Oda, Y. and Minami, K. (1986) Eur. J. Biochem. 159, 239-245]. The other agglutinin is a new one which agglutinates animal erythrocytes and was purified from the tulip bulbs using affinity chromatography on thyroglobulin-Sepharose 4B. The agglutinin agglutinated mouse and rat erythrocytes at a minimum concentration of 2 micrograms/ml and 30 micrograms/ml respectively, but did not agglutinate erythrocytes from other animals and yeasts even at a concentration of 1000 micrograms/ml. The agglutinin appeared homogeneous by disc gel electrophoresis at pH 4.3 and gel filtration. Its relative molecular mass was determined by gel filtration to be approximately 40,000. It was suggested that the agglutinin was composed of two different subunits of 26 kDa and 14 kDa by sodium dodecyl sulfate/polyacrylamide gel electrophoresis analysis. Binding of radioiodinated agglutinin to mouse erythrocytes indicated that the presence of a high-affinity site with a dissociation constant of 2.00 X 10(-9) M. In inhibition experiments thyroglobulin glycopeptides were the most potent inhibitors; thyroglobulin was also a potent inhibitor. Orosomucoid and mucin showed weak inhibition. The other glycoproteins, glycopeptides and sugars examined showed no inhibition.
D000372:Agglutination Tests; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D006801:Humans; D007525:Isoelectric Focusing; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D037121:Plant Lectins; D010944:Plants / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D011975:Receptors, Mitogen / Q000378:metabolism; D012756:Sheep
D000596:Amino Acids; D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; C052928:Tulipa gesneriana agglutinin