Mercaptoethanol assists in cleaving a cysteine/cystine-free protein into its subunits.
The alpha-mannosidase from Canavalia ensiformis is devoid of cysteine and cystine. Nevertheless, its cleavage into subunits is facilitated by the presence of mercaptoethanol in the denaturing buffer.
D003545:Cysteine / Q000378:metabolism; D003553:Cystine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D008361:Mannosidases / Q000378:metabolism*; D008623:Mercaptoethanol / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism*; D010946:Plants, Medicinal; D012639:Seeds / Q000201:enzymology*; D043323:alpha-Mannosidase
D010940:Plant Proteins; D003553:Cystine; D008623:Mercaptoethanol; D008361:Mannosidases; D043323:alpha-Mannosidase; D003545:Cysteine