Isolation and characterization of a mucin-type glycoprotein from the lung lavage of a patient with alveolar proteinosis.
A high molecular weight glycoprotein was isolated from the lavage fluid of a patient with alveolar proteinosis by gel chromatography with Sepharose CL-4B. The glycoprotein gave a single band stainable with alcian blue and with periodate-Schiff reagent on the cellulose acetate membrane electrophoresis. The glycoprotein did not penetrate 3.3% polyacrylamide gel but moved into 1% agarose gel as a periodate-Schiff positive single band, when electrophoresed in the presence of sodium dodecyl sulfate. The chemical analysis and the results of the beta-elimination reaction showed the presence of O-linked carbohydrate chains characteristic for a mucin-type glycoprotein. These data provide the first characterization of a mucin-type glycoprotein isolated from lung in pulmonary alveolar proteinosis.
D000596:Amino Acids / Q000032:analysis; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D004586:Electrophoresis; D006801:Humans; D009077:Mucins / Q000032:analysis*; D011649:Pulmonary Alveolar Proteinosis / Q000378:metabolism*; D007507:Therapeutic Irrigation
D000596:Amino Acids; D002241:Carbohydrates; D009077:Mucins