Increased norepinephrine sensitive intracellular Ca2+ pool in the caudal artery of spontaneously hypertensive rats.
The contractions evoked by norepinephrine (NE) and caffeine in Ca2+-free solution were determined using denervated caudal artery rings from normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). The magnitude of contractions produced by different concentrations of NE was significantly greater (P less than 0.05) in SHR caudal artery rings compared to WKY. The contractions evoked by NE in Ca2+-free solution were mediated primarily through the activation of postsynaptic alpha 1-adrenoceptors. In addition to alpha 1-adrenoceptor stimulation, caffeine also evoked significantly greater (P less than 0.05) contractions in Ca2+-free solution in SHR caudal arteries compared to WKY. From these observations it is concluded that intracellular Ca2+ pool (presumably sarcoplasmic reticulum, SR) is increased in SHR caudal arteries which, at least in part, may account for the increased contraction observed in response to NE and caffeine stimulation in the absence of extracellular Ca2+.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000097:blood*; D002413:Cations, Divalent; D008297:Male; D008729:Methoxamine / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000503:physiopathology*; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D002413:Cations, Divalent; D002110:Caffeine; D008729:Methoxamine; D002118:Calcium; D009638:Norepinephrine