Cytogenetic changes of human peripheral blood lymphocytes in vitro after exposure to cis-DDP (cis-diamminedichloroplatinum II) and oxo-Pt (cis-diamminedichloro-trans-dihydroxyplatinum IV).
Human peripheral blood lymphocytes were tested for induction of micronuclei, chromosome aberrations and sister chromatid exchanges (SCEs) frequency after exposure to two platinum complexes (cis-DDP Platidiam and oxo-Pt) and a comparison was made with the inhibitory effect of these drugs on mitotic activity. When the cis-DDP cell samples were compared with the untreated controls, there was a distinct increase in the frequency of micronuclei and chromosome aberrations, and the statistically significant increase in SCE frequency was accompanied by a significant decrease in mitotic activity. In oxo-Pt cell samples, using an identical concentration of the drug, only a slight increase in micronuclei and chromosome aberration frequency was observed. However, the increase in the SCE frequency was not significant and neither was the decrease of mitotic activity when compared with the controls.
D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D002945:Cisplatin / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D012854:Sister Chromatid Exchange
D002945:Cisplatin