title: The "manganese(III)-containing" purple acid phosphatase from sweet potatoes is an iron enzyme.
abstract: An improved purification of the purple acid phosphatase from sweet potatoes has been developed, and the properties of the enzyme have been reexamined. Contrary to previous reports, (e.g., Y. Sugiura, et al., J. Biol. Chem., 256, 10664-10670 (1981) ), the enzyme contains two moles of iron and insignificant amounts of manganese. The specific activity of the iron-containing preparations is ca. 14 times higher than that reported previously for the purported "Mn(III)" enzyme. The sweet potato purple acid phosphatase does indeed bind manganese, but it can be removed by dialysis with no changes in specific activity or spectral properties.
mesh_terms: D000135:Acid Phosphatase / Q000302:isolation & purification* / Q000378:metabolism; D007501:Iron / Q000032:analysis; D007700:Kinetics; D010944:Plants / Q000201:enzymology*; D011198:Solanum tuberosum
chemical_list: D007501:Iron; D000135:Acid Phosphatase