Interaction of promazine with human ceruloplasmin.
Promazine is enzymically oxidized by ceruloplasmin without reduction of the 610 nm absorption band of the enzyme. Fluoride inhibited the reaction in a non-competitive manner. The ceruloplasmin oxidase activity is markedly enhanced when promazine is added in the presence of NADH; possibly through a change in enzyme conformation.
D002570:Ceruloplasmin / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009243:NAD / Q000494:pharmacology; D010084:Oxidation-Reduction; D011395:Promazine / Q000378:metabolism*; D013053:Spectrophotometry
D009243:NAD; D002570:Ceruloplasmin; D011395:Promazine; D005459:Fluorides