Endogenous serotonin release from rat cerebellar slices.
Endogenous serotonin (5-HT) content in cerebellar slices and in the incubation medium was measured before and after depolarizing stimulus with high-K+ concentration in the medium. Small amount of 5-HT was spontaneously released from tissue slices incubated in low-K+ and Ca2+-free medium. A significant increase of endogenous 5-HT release was obtained after depolarizing stimulus with high-K+ plus Ca2+ in the medium. It is suggested that the endogenous 5-HT release in cerebellum induced by depolarizing stimuli is a Ca2+-dependent phenomenon.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002531:Cerebellum / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D011188:Potassium; D002118:Calcium