Pattern of Na-K-ATPase inhibitors in plasma and urine of hypertensive patients: a preliminary report.
The pattern of endogenous Na-K-ATPase inhibitors in plasma and urine from patients with essential hypertension and normal controls was established by sequential Amicon ultrafiltration through membranes with molecular weight cut-offs of 50 K daltons, 30 K daltons and 1 K daltons, followed by C18 Sep-Pak cartridge separation of the 1 K filtrate. Significantly increased Na-K-ATPase inhibitory activity in the hypertensive population was found in a high molecular weight (30-50 K daltons) urine fraction, one low molecular weight (less than 1 K daltons) urine fraction and two low molecular weight plasma fractions. Preliminary evidence is presented to suggest that large molecular weight plasma fractions may contain a "masked" Na-K-ATPase inhibitor, in either carrier or precursor form.
D001798:Blood Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration / Q000379:methods; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000652:urine; D008970:Molecular Weight; D011506:Proteins; D011507:Proteinuria; D012016:Reference Values; D000097795:ATPase Inhibitory Protein
D001798:Blood Proteins; D011506:Proteins; D000097795:ATPase Inhibitory Protein