Use of elevated pressure to promote the difference in permeability of Adriamycin and hematoporphyrin between neoplastic and normal lung cells.
The effect of increased pressure on the permeability of Adriamycin into human lung cells was studied as a function of a combination of pressure and temperature. Normal diploid lung cells as well as cells with adenocarcinoma were subjected to elevated pressures of up to 225 bars. The penetration of Adriamycin and hematoporphyrin into the cells, were assayed by use of fluorescence and absorption spectroscopy. After cells were subjected to pressure in the presence of Adriamycin, there was a greater concentration of Adriamycin in malignant cells than in normal cells. Malignant cells subjected to a pressure of 8 bars, for 210 min at 37 degrees C were found to contain twice the concentration of Adriamycin than normal cells, under the same conditions. The present results suggest that elevated pressure techniques may serve to increase the permeability of malignant cells to antineoplastic drugs.
D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004317:Doxorubicin / Q000008:administration & dosage* / Q000378:metabolism; D006415:Hematoporphyrins / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D006874:Hydrostatic Pressure; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D011312:Pressure; D013696:Temperature
D006415:Hematoporphyrins; D004317:Doxorubicin