Determination of succinyldicholine in different tissue samples from guinea pigs after injection of a single intravenous dose.
The distribution and postmortem stability of succinyldicholine in different tissues and urine from guinea-pigs has been studied. Succinyldicholine was extracted from tissue homogenates and urine samples from animals sacrificed by intravenous injections of succinyldicholine hydrochloride (40 mg/kg). The bis-quaternary ammonium compound was demethylated and the tertiary amine was analysed by gas chromatography/mass spectrometry. The concentrations found in muscle, kidney and urine were often low; in muscle below 5 pmol/g, in kidney from 5 to 1500 pmol/g and in urine from 5 to 650 pmol/ml. The eye proved to be the best tissue sample, with a rather high and constant concentration (280 +/- 36 pmol/g) of succinyldicholine. The postmortem stability was studied by storing the bodies at 4 degrees C. After 6 days storage the drug concentrations in the eyes started to decline. Four weeks after death it was not possible to detect any succinyldicholine in this tissue.
D000818:Animals; D005123:Eye / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006168:Guinea Pigs; D007275:Injections, Intravenous; D007668:Kidney / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry; D009132:Muscles / Q000032:analysis; D013390:Succinylcholine / Q000032:analysis* / Q000652:urine
D013390:Succinylcholine