Isolation of viable mouse primordial germ cells by antibody-directed flow sorting.
The germ line represents a cell type of unique interest in mammals because it retains complete genotypic totipotency while undergoing significant phenotypic differentiation. Analysis of the mechanism that underlies the maintenance of this totipotency requires the ability to isolate and study all stages of the germ cell lineage. The primordial germ cells (PGC) are the earliest identifiable germ cells in the embryo. It has not previously been possible to isolate PGC in sufficient numbers and purity to facilitate biochemical and/or molecular analysis. We report here that the use of a monoclonal antibody in combination with flow cytometry does permit the isolation of reasonably large and pure yields of viable mouse PGC.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002469:Cell Separation / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D005854:Germ Cells; D006066:Gonads / Q000166:cytology; D051379:Mice
D000911:Antibodies, Monoclonal