Therapeutic drug monitoring in a pediatric laboratory.
Therapeutic drug monitoring using a new and faster high pressure liquid chromatographic (HPLC) method is discussed. Analysis of chloramphenicol and succinate esters and a large number of anticonvulsive drugs can be carried out using the same buffer and reverse-phase HPLC column by changing the solvent concentration to reflect the choice of drugs. A buffer prepared from 0.02 M sodium acetate and trifluoroacetic acid provides an optimal mobile phase for separation of a wide variety of drugs commonly used in pediatric medicine. Sample preparation and the use of special internal standards without extensive extractions provides excellent accuracy, reliability and sensitivity, and requires a minimum of preparation.
D000927:Anticonvulsants / Q000097:blood; D002110:Caffeine / Q000097:blood; D002648:Child; D002701:Chloramphenicol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008991:Monitoring, Physiologic / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000097:blood*; D013806:Theophylline / Q000097:blood
D000927:Anticonvulsants; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D002110:Caffeine; D002701:Chloramphenicol; D013806:Theophylline