Isolation of ketoses or nonreducing sugars from a sugar mixture containing aldoses by aniline treatment.
A method for the isolation of ketoses and nonreducing sugars from sugar mixtures by the addition of aniline, in the presence of acetic acid as a catalyst, is described. The aldoses react readily to form aniline derivatives, which are then removed using standard procedures: extraction with organic solvents, and treatment with activated charcoal and with a cation-exchange resin. The ketoses and nonreducing sugars are retained in aqueous solution.
D000085:Acetates; D000814:Aniline Compounds; D002241:Carbohydrates / Q000302:isolation & purification*; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D005947:Glucose; D006207:Half-Life; D007661:Ketoses / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D012997:Solvents
D000085:Acetates; D000814:Aniline Compounds; D002241:Carbohydrates; D007661:Ketoses; D012997:Solvents; D005947:Glucose; C023650:aniline