Sodium chloride-esculin hydrolysis test for rapid identification of enterococci.
The ability of enterococci to cause severe disease in humans and their relative resistance to chemotherapeutic agents make it desirable to rapidly differentiate these organisms from other streptococci. We developed and evaluated a test that within 2 h distinguishes enterococci from other alpha-, beta-, or nonhemolytic streptococci in a buffered solution containing 0.2% esculin and 5% sodium chloride. All 239 strains of enterococci tested gave a positive reaction within 2 h, whereas 95 of 96 isolates of other streptococci remained negative at 4 h.
D004929:Esculin / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012965:Sodium Chloride / Q000494:pharmacology*; D013291:Streptococcus / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
D005419:Flavonoids; D004929:Esculin; D012965:Sodium Chloride