title: Intracellular sodium ion activity: reliable measurement and stimulation-induced change in cardiac Purkinje fibers.
abstract: Recently Na+-selective microelectrodes (NaSM) have been used to measure quantitatively small changes in intracellular sodium ion activity (aiNa) and to determine a precise time course of comparatively rapid change in aiNa. In such studies, accurate measurement of aiNa requires the following criteria: (i) NaSM should have a fast response time and (ii) an NaSM and a conventional voltage microelectrode should measure the same membrane potential. These criteria were evaluated by measuring aiNa when membrane potential of cardiac Purkinje fibers was suddenly hyperpolarized and depolarized by changing stimulation rate. The NaSM coated with a conductive silver paint had fast response times so that rapid changes in aiNa could be reliably measured. The cardiac Purkinje fibers stimulated at a constant rate generated uniform membrane voltage and the NaSM and conventional microelectrode measured virtually the same membrane potential. This result is somewhat different from that reported under voltage-clamp condition by other investigators. The aiNa of the fibers increased as the stimulation rate was increased over the range of 0.5-3 Hz. In fibers stimulated at 1 Hz, cessation of stimulation was immediately followed by an exponential decline of aiNa with an average time constant of 53 +/- 9 s (SD, n = 8), or rate constant of 0.020 +/- 0.004/s. Restimulation of the fibers produced an exponential rise of aiNa with an average time constant of 65 +/- 12 s (n = 8). Similar results were obtained in fibers stimulated at 2 Hz.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D004285:Dogs; D004558:Electric Stimulation; D006329:Heart Conduction System / Q000378:metabolism*; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D011690:Purkinje Fibers / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013327:Strophanthidin / Q000494:pharmacology
chemical_list: D013327:Strophanthidin; D012964:Sodium