title: Serological survey for Toxoplasma infections in sheep.
abstract: A factorial-design survey was performed to determine the prevalence of specific antibody against Toxoplasma in young and adult sheep from 6 areas in 3 different geoclimatic zones in South Australia. Serum samples obtained from 1,159 sheep belonging to 59 flocks were tested by a conventional indirect haemagglutination test (IHAT) as well as by an enzyme-linked immunosorbent assay (ELISA) which used class-specific conjugates to detect both IgG and IgM against Toxoplasma. Titres greater than 64 were detected in 7.4%, 9.2% and 25.2% of the sheep by the IHAT, IgG-ELISA and IgM-ELISA respectively. A significant positive correlation was found between the results of the IgG-ELISA and the IHAT with identical results obtained for 1,050 samples. Antibody detected by all 3 tests was more prevalent and higher in titre in adult sheep than in lambs as well as in sheep from Kangaroo Island than in those from mainland South Australia. Although the regional differences in prevalence suggested a relationship with climate, no significant correlations were detected between the prevalence results and any single climatic factor; namely, average annual rainfall, average annual evaporation or mean temperature range.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D001315:Australia; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology* / Q000276:immunology
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M