[Purification of human plasma fibronectin by double affinity chromatography on gelatin and heparin-Trisacryl].
In this work the human plasma fibronectin was purified by affinity chromatography using a tandem column system. The first affinity column was filled with gelatin-Trisacryl whereas the second one contained heparin-Trisacryl. This double affinity chromatography demonstrated its high efficiency in term of purity and yield. Several analytical methods (electrophoresis, immunoelectrophoresis, F.P.L.C. and adhesion assay on cultured eucaryotic cells) evidenced in fact the high purity of the preparation as well as its biological behaviour in term of cell adhesion and spreading. The performances of the sorbents used facilitate the scaling up when large quantities of FNP are needed.
D000180:Acrylic Resins; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods; D005353:Fibronectins / Q000097:blood* / Q000302:isolation & purification; D006493:Heparin; D006801:Humans
D000180:Acrylic Resins; D005353:Fibronectins; D006493:Heparin
Purification de la fibronectine plasmatique humaine par double chromatographie d'affinité sur gélatine et héparine-trisacryl.