Isolation of alpha-crystallin subunits by gel filtration.
In the presence of 0.1 M glycine, pH 2.5, alpha-crystallin dissociates into aggregates containing only alpha A chains plus monomeric alpha B chains. Advantage has been taken of this to develop a method for the purification of the alpha A and alpha B chains by gel filtration on Sephadex G-75. This method gives high yields of purified subunits and avoids the risk of carbamylation associated with other methods which use high concentrations of urea.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D003459:Crystallins / Q000302:isolation & purification*; D008722:Methods
D003459:Crystallins