[Reactive chemiluminescence of human lymphocytes during phytohemagglutinin stimulation].
The luminol-mediated chemiluminescence of highly purified human peripheral blood lymphocytes has been studied. The incubation of lymphocytes with phytohemagglutinin has lead to dose-dependent chemiluminescence, most pronounced by the end of the first minute. The kinetics of the reaction does not depend on the dose of the stimulant, the concentration of lymphocytes or the presence of mononuclear phagocytes. Monocytes (adhering cells) are not stimulated with phytohemagglutinin though their presence in the total fraction of mononuclear blood cells enhances the chemiluminescence of lymphocytes.
D002469:Cell Separation / Q000379:methods; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013997:Time Factors
D010835:Phytohemagglutinins; D008165:Luminol
Reaktivnaia khemiliuminestsentsiia limfotsitov cheloveka pri stimuliatsii fitogemaggliutininom.