Effects of storage on the pyruvate-lactate system and random migration of human granulocytes.
This work demonstrates a time-dependent loss of polymorphonuclear neutrophilic leukocyte (PMN) functions during the first 48 h with only slight lysis and release of cellular LDH. It confirms that storage decreases ATP levels in PMNs and significantly increases intracellular lactate and pyruvate. These data correlated with an inhibition of PMN migratory function and a considerable decrease in chemiluminescence response.
D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation; D002465:Cell Movement; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D009504:Neutrophils / Q000502:physiology*; D011773:Pyruvates / Q000097:blood*; D019289:Pyruvic Acid
D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase