In vitro ADP-ribosylation of nuclear proteins in mouse tissues. Identification of an unusual group of acceptors in testis nuclei.
1. Acceptor proteins for poly(ADP-ribose) have been identified in nuclei from mouse testis, liver, kidney and spleen. Purified nuclei were incubated in vitro with [14C]NAD, extracted sequentially with 5% HClO4 and 0.25 N-HCl and labelled proteins analysed on acetic acid/urea polyacrylamide gels pH 2.9. 2. Results show that: (a) in vitro there are significant differences between tissues in the extent of poly(ADP-ribosylation) of nuclear proteins; (b) in testis nuclei two tissue specific proteins are poly(ADP-ribosylated) to higher specific activity than histones; (c) there are significant differences between in vivo and in vitro studies on poly(ADP-ribosylation) of nuclear proteins in testis nuclei.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis / Q000378:metabolism*; D002843:Chromatin / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D013737:Testis / Q000378:metabolism* / Q000648:ultrastructure
D002843:Chromatin; D006657:Histones; D011506:Proteins; D000246:Adenosine Diphosphate Ribose