Cell lineage analysis of the primitive and visceral endoderm of mouse embryos cultured in vitro.
Cell lineages of the primitive endoderm and the visceral endoderm of mouse embryos were examined by culturing whole embryos in vitro. The primitive endoderm and visceral endoderm cells could be labelled by incubation of embryos in a medium containing horse radish peroxidase (HRP). HRP localization was chased throughout the culture period. The results show that the visceral endoderm derives from the primitive endoderm, and the visceral endoderm forms only the extra-embryonic endoderm (yolk sac endoderm) of the conceptus. The definitive endoderm which is probably derived from the head process, newly appears on the ventral surface of the embryo.
D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D046508:Culture Techniques; D004707:Endoderm / Q000166:cytology*; D006735:Horseradish Peroxidase; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013997:Time Factors; D015017:Yolk Sac / Q000166:cytology
D006735:Horseradish Peroxidase