Primary structure of O- and N-glycosylic carbohydrate chains derived from murine submandibular mucin (MSM).
The carbohydrate moiety of mouse submandibular mucin (MSM) contains mainly D-mannose and 2-acetamido-2-deoxy-D-glucose together with sialic acid, D-galactose, and 2-acetamido-2-deoxy-D-galactose. O-Glycosylically bound saccharides, obtained by treatment of MSM with alkaline borohydride, were shown by methylation analysis to have the structure: alpha-NeuAc-(2----3)-beta-Gal-(1----3)-GalNAc-ol. N-Glycosylically bound saccharides obtained from MSM by hydrazinolysis, and analysed by 500-MHz 1H-n.m.r. spectroscopy, were shown to have the following comprehensive structures. (Formula: see text).
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006027:Glycosides; D007202:Indicators and Reagents; D008745:Methylation; D051379:Mice; D009077:Mucins / Q000302:isolation & purification; D013363:Submandibular Gland / Q000032:analysis*
D006027:Glycosides; D007202:Indicators and Reagents; D009077:Mucins