Adenosine potentiation and antagonism may account for the diverse behavioral actions of Ro 5-4864.
The actions of the p-chloroderivative of diazepam, Ro 5-4864 on the spontaneous discharges of rat cerebral cortical neurons and its interactions with depressions evoked by adenosine and adenosine 5'-N-ethylcarboxamide (NECA) were observed. Iontophoretically applied Ro 5-4864 had variable actions on cortical neuronal activity, exciting some neurons and depressing others. During the period of applications. Ro 5-4864 antagonized the depressant effects of NECA, whilst having less of an action on adenosine depressions. Following Ro 5-4864 application adenosine, but not NECA, depressions were potentiated in amplitude and duration for periods of up to 20 min. It is suggested that Ro 5-4864 has both antagonistic and potentiative interactions with adenosine.
D000200:Action Potentials / Q000187:drug effects; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology*; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000502:physiology; D003292:Convulsants / Q000494:pharmacology*; D007478:Iontophoresis; D008297:Male; D009433:Neural Inhibition / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D001570:Benzodiazepinones; D003292:Convulsants; C028513:4'-chlorodiazepam; D019830:Adenosine-5'-(N-ethylcarboxamide); D000241:Adenosine