[Effect of temperature on thrombin- and trypsin-catalyzed hydrolysis of synthetic substrates].
A comparative analysis of the temperature effect on the thrombin- and trypsin-catalyzed hydrolysis of synthetic substrates which are derivatives of A(alpha)-chain of fibrinogen has been carried out. The substrates have general formula: Tos-P2-Arg-OCH3 and Tos-P3-P2-Arg-OCH3, where P2-Gly, Val, MeVal; P3 = Gly, Sar. The activation parameters delta G not equal to, delta H not equal to and delta S not equal to are determined. Thrombin is shown to split the most effectively tripeptide containing amino acids sequence Gly-Gly at the subsites of P3 and P2. It is suggested to be caused by an ability of the above compound to take a bent conformation at the active site of thrombin.
D000596:Amino Acids; D001665:Binding Sites; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D013379:Substrate Specificity; D013696:Temperature; D013917:Thrombin / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*
D000596:Amino Acids; D005340:Fibrinogen; D014357:Trypsin; D013917:Thrombin
Vliianie temperatury na kataliziruemyĭ trombinom i tripsinom gidroliz sinteticheskikh substratov.