Cloning and identification of lupin nodule specific cDNA sequences.
Yellow lupin nodule specific sequences were selected by screening of cDNA library prepared from lupin nodule poly(A)+RNA. From about 3,000 clones containing fragments of lupin DNA 150-1,500 base pair long, 7% of clones carrying nodule specific sequences were identified. Among them the most abundant sequence species, represented by 32% clones, encodes leghemoglobin. Another abundant species designated pLN13 is represented by 13% clones. The Northern blot analysis of lupin mRNA confirmed nodule specificity of the cloned sequences. The nucleotide sequence of one clone, pLN281 of 225 bp, is presented.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000235:genetics* / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000235:genetics; D012231:Rhizobium
D012333:RNA, Messenger; D004247:DNA