DNA hybridization assay for detection of Salmonella in foods: collaborative study.
A collaborative study was performed in 11 laboratories to validate a DNA hybridization (DNAH) procedure for detection of Salmonella in foods. The DNAH procedure was compared to the standard culture method for detection of Salmonella in 6 foods: ground pepper, soy flour, dry whole egg, milk chocolate, nonfat dry milk, and raw deboned turkey. With the exception of turkey which was naturally contaminated, uninoculated and inoculated samples of each food group were analyzed. Results for the DNAH method were significantly better than for the standard culture method at the 5% probability level for the detection of Salmonella in turkey. There was no significant difference between the methods for the other 5 foods. The method has been adopted official first action.
D000818:Animals; D002099:Cacao / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D003611:Dairy Products / Q000032:analysis; D004531:Eggs / Q000032:analysis; D005374:Filtration; D005516:Food Microbiology; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D013025:Glycine max / Q000032:analysis; D014422:Turkeys
D004269:DNA, Bacterial; D007202:Indicators and Reagents