A simple, non-chromatographic purification procedure for monoclonal antibodies. Isolation of monoclonal antibodies against cytochrome P450 isozymes.
A simple, non-chromatographic purification procedure for monoclonal antibodies from mouse ascites fluid is described. This procedure, which is rapid, inexpensive, and has high capacity involves the precipitation of contaminating proteins with caprylic acid followed by precipitation of immunoglobulin using ammonium sulfate. This two-step procedure is shown to be effective for the purification of various immunoglobulins including IgG1, IgG2a and IgG2b. In the present report, more than 30 monoclonal antibodies directed against cytochrome P450 isozymes have been purified by this method and characterized.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D001202:Ascitic Fluid / Q000276:immunology; D002210:Caprylates / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology*; D007074:Immunoglobulin G / Q000145:classification; D007527:Isoenzymes / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D002210:Caprylates; D007074:Immunoglobulin G; D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; C031492:octanoic acid