Interaction of the voltage-sensing fluorescent probe diS-C3-(5) with dipalmitoylphosphatidylcholine liposomes.
The interaction of the probe diS-C3-(5) with dipalmitoylphosphatidylcholine (DPPC) liposomes has been studied using fluorescence and differential scanning calorimetry (DSC). The partition coefficients (K) of the probe for the lipid and the aqueous phase (in terms of molar part units) were (1.20 +/- 0.4) X 10(6) at 45 degrees C and (0.50 +/- 0.07) X 10(6) at 23 and 36 degrees C. In terms of volume concentration units, these values correspond to Kp = (2.88 +/- 0.10) X 10(4) and Kp = (1.20 +/- 0.17) X 10(4), respectively. DSC thermograms were practically identical both for large unilamellar and multilamellar liposomes. The main transition peak remained practically unchanged over the entire range of the probe concentrations used. The pretransition could be observed up to maximal probe concentrations applied and it widened and shifted from 35.4 degrees C in pure DPPC to approximately 32 degrees C at a probe/lipid ratio of 0.027. These results suggest that in both quasicrystalline and liquid crystalline lipid bilayers the probe molecules are included in "defects" between structurally ordered microregions (microdomains or clusters). The dependence of the fluorescence response on the transmembrane potential in a suspension of unilamellar DPPC vesicles suggest that the equilibrium thermodynamic model is valid for liquid crystalline bilayers.
D015060:1,2-Dipalmitoylphosphatidylcholine; D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007700:Kinetics; D008081:Liposomes; D008564:Membrane Potentials; D008954:Models, Biological; D011804:Quinolines; D013050:Spectrometry, Fluorescence
D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D008081:Liposomes; D011804:Quinolines; D015060:1,2-Dipalmitoylphosphatidylcholine; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine