title: Nitrogen flux across ovine maternal and fetal hindquarters during fasting.
abstract: Nitrogen flux across the hindquarters of fetal and maternal sheep (n = 15) was determined during normal feeding and following 5 days of maternal fasting. Arterial and venous whole blood concentrations of free amino acids, ammonia and oxygen were measured entering and exiting the hindquarters. Further, the DNA, protein and nitrogen contents of the hindlimb skeletal muscle of the fetus were determined in the fed state and following the 5-day fast. Results of these studies indicate that maternal and fetal hindlimb metabolism differ during fasting. There is a net efflux of alanine, glutamine and total nitrogen from the maternal hindquarters following 5 days of fasting. The fetus also releases glutamine and alanine from the hindquarters during the fast, presumably as potential energy substrate. However, nitrogen balance across the fetal hindquarter remains positive as a result of increased positive arteriovenous differences for other amino acids (particularly leucine and isoleucine). The concentrations of DNA, protein and nitrogen in fetal skeletal muscle remain unchanged during fasting. These data indicate that, whereas the mother undergoes protein catabolism and net nitrogen loss from the hindquarter during fasting, the fetus maintains a positive nitrogen balance across the hindquarter.
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D005215:Fasting; D005260:Female; D006614:Hindlimb / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D009132:Muscles / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D011247:Pregnancy; D012756:Sheep
chemical_list: D000596:Amino Acids; D009584:Nitrogen; D010100:Oxygen