Respiration of bull spermatozoa in presence of exogenous glutathione (GSH).
In the investigations of the rate of oxygen uptake by sperm cells in frozen bull semen it was found that exogenous glutathione (GSH) in a 5 mM concentration failed to stimulate mitochondrial oxidative processes in the sperm cells. At the same time it was observed that the respiration of sperm cells changed in relation to the season of the year when ejaculates had been collected and preserved. The highest values were noted in winter and the lowest ones in summer. Moreover, in the observations on the motility of spermatozoa a difference was observed in the reaction of sperm cells to exogenous GSH, and this difference depended on the season. A significant (p less than or equal to 0.05) rise was disclosed in the number of sperm cells with maintained advancing motility in the presence of GSH in the medium as compared to controls without GSH, but this rise was found only in semen obtained and preserved in winter.
D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects*; D012621:Seasons; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000378:metabolism*
D005978:Glutathione