title: A simple procedure for reducing sampling problems in the transmission electron microscope diagnosis of human tumors.
abstract: A simple procedure is described which permits more extensive sampling in the transmission electron microscopic analysis of human tumor tissue. Thin slices of 2 to 4 mm side are cut from fresh or fixed specimens and processed for embedding in exactly the same manner as for conventional mm-cubes pieces, with blocking out in 8 mm-diameter flat-bottomed molds. Advantages and shortcomings of the technique compared with other published methods having the same objective, are discussed. Examples are given to illustrate the usefulness of the procedure.
mesh_terms: D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis* / Q000648:ultrastructure; D013048:Specimen Handling / Q000379:methods