title: Effect of parbendazole and piperazine adipate on the activity of some enzymes of Ascaridia galli and Heterakis gallinae.
abstract: Adult Ascaridia galli and Heterakis gallinae obtained from the fowl (Gallus gallus) were treated in vitro with 10(-2) to 10(-5) M parbendazole and piperazine adipate for 10-60 min at 38 degrees C. Both the compounds at 10(-2) M caused mortality of A. galli and H. gallinae after a maximum of 30 min exposure. The effect of the drugs on the homogenates of the treated worm was investigated. Parbendazole (10(-2) M) inhibited malate oxidation by 68% in A. galli and 62% in H. gallinae. Piperazine adipate (10(-2) M) inhibited malate oxidation by 78% in both parasites. In A. galli oxaloacetate reduction was inhibited by 41 and 26% by 10(-2) M parbendazole and piperazine adipate, respectively; with H. gallinae this inhibition was found to be 39 and 55%, respectively. Aldolase activity in both the parasites was also inhibited by 10(-2) M parbendazole and piperazine adipate. Both compounds caused an inhibition of acid phosphomonoesterase activity, but the activities of lactate dehydrogenase and alkaline phosphomonoesterase were not affected significantly. Parbendazole (10(-2) M) had no significant effect on the cholinesterase activity of these parasites, but piperazine adipate (10(-2) M) caused an inhibition of 96% in A. galli and 93% in H. gallinae. The possible mode of action of the drugs is discussed.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D001197:Ascaridia / Q000187:drug effects* / Q000201:enzymology; D001199:Ascaridoidea / Q000187:drug effects* / Q000201:enzymology; D001562:Benzimidazoles / Q000494:pharmacology*; D002802:Cholinesterases / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D000077489:Piperazine; D010879:Piperazines / Q000494:pharmacology*
chemical_list: D000969:Antinematodal Agents; D001562:Benzimidazoles; D010879:Piperazines; D000077489:Piperazine; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D002802:Cholinesterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005634:Fructose-Bisphosphate Aldolase; C100292:parbendazole