beta-D-mannosidase in human polymorphonuclear leukocytes and lymphocytes: a comparative study.
All lymphocytes and polymorphonuclear leukocytes (PMNL) beta-D-mannosidase activities are adsorbed on DEAE-Trisacryl column at pH 7.0. Only one form is eluted with a 0.15 M linear gradient. The two enzymes isolated from either type of cells exhibit similar properties. The chromatographic profiles of beta-D-mannosidase from leukemic lymphocytes (chronic lymphoïd leukemia and hairy cells leukemia) differ from the normal ones by the presence of a more acidic minor form.
D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D008361:Mannosidases / Q000097:blood*; D009504:Neutrophils / Q000201:enzymology*; D044902:beta-Mannosidase
D008361:Mannosidases; D044902:beta-Mannosidase