In vitro synthesis and processing of a bean pathogenesis-related (PR4) protein.
When bean plants (Phaseolus vulgaris var. Saxa) are treated with mercuric chloride or infected with alfalfa mosaic virus, they produce pathogenesis-related (PR) proteins. We report here that functional mRNA encoding bean PR4 protein is only present when synthesis of this protein has been induced. Treatment with mercuric chloride results in a rapid induction of functional bean PR4 mRNA (within 2-3 h), whereas in virus-infected plants this mRNA can only be detected the second day following the infection. Bean PR4 protein is synthesized in vitro, using this mRNA in a rabbit reticulocyte lysate system, as a precursor of 35 kDa. This precursor can be processed into a polypeptide having the same molecular mass (33.5 kDa) as the in vivo PR4 protein by the addition to the cell-free translation system of canine pancreatic microsomal membranes.
D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000378:metabolism*; D007120:Immunochemistry; D066298:In Vitro Techniques; D000455:Medicago sativa; D008627:Mercuric Chloride; D009029:Mosaic Viruses; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D010946:Plants, Medicinal; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism
D010940:Plant Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; C053376:pathogenesis-related proteins, plant; D008627:Mercuric Chloride