title: DNA rearrangements associated with instability of an arginine gene in Streptomyces coelicolor A3(2).
abstract: Streptomyces coelicolor A3(2) gives rise to spontaneous chloramphenicol sensitive mutants at a frequency of about 0.3% per spore. These mutants are often genetically unstable and give rise to arginine auxotrophs (Arg-) at frequencies of 1-7% per spore. These Arg- mutants usually lack the enzyme argininosuccinate synthetase (one exception was found that lacked ornithine carbamoyltransferase) and were shown to have deleted the corresponding argG gene by hybridisation analysis using a cloned S. cattleya argG gene. The Arg- strains also showed a variety of different DNA amplification and deletion events in a region homologous to an amplified DNA sequence found in spontaneous Arg- mutants in S. lividans 66.
mesh_terms: D001120:Arginine / Q000096:biosynthesis*; D001124:Argininosuccinate Synthase / Q000235:genetics*; D002701:Chloramphenicol / Q000494:pharmacology; D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial; D005784:Gene Amplification; D005798:Genes, Bacterial; D008025:Ligases / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D002701:Chloramphenicol; D001120:Arginine; D008025:Ligases; D001124:Argininosuccinate Synthase