title: Comparative studies of acid and alkaline phosphatase activities in nonvascular smooth muscle membranes.
abstract: Acid and alkaline phosphosphatase activities of subcellular fractions isolated from rat gastric muscle and vas deferens by differential centrifugation, sucrose density gradient and cation-induced aggregation methods were studied using p-nitrophenyl phosphate as the substrate. Alkaline phosphatase and a large portion of acid phosphatase activities were found to be of plasmalemmal origin. Acid and alkaline phosphatase activities were different in the effect of Mg2+, fluoride, vanadate, EDTA and resistance to heat inactivation suggesting that these two phosphatase activities were not expressed by the same enzyme.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000201:enzymology; D009130:Muscle, Smooth / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000201:enzymology; D014649:Vas Deferens / Q000201:enzymology
chemical_list: D000469:Alkaline Phosphatase; D000135:Acid Phosphatase