Effects of ascorbic acid supplementation on liver, lung and kidney microsomal drug metabolizing enzymes of the guinea-pig.
1. Male guinea-pigs were administered 8, 180 and 360 mg ascorbic acid/day via drinking water for 1 and 2 months. The two high levels of ascorbic acid were not able to produce saturation in either blood or any of the three tissues (liver, lung and kidney) while the lowest ascorbic acid level was sufficient to prevent scurvy. 2. There was no significant differences among the groups receiving three distinct ascorbic acid levels in body weights, tissue weights or protein contents. 3. No significant alterations were observed in microsomal ethylmorphine N-demethylase activity of any of the three tissues among the groups after experimental periods. 4. The two high ascorbic acid levels produced significant increases in liver microsomal aniline 4-hydroxylase activity as compared to the lowest supplementation of ascorbic acid after 2 months. However, no significant enzyme activity changes were found among the groups after 1 month. 5. In lung, after 1 month significant increase was observed in microsomal aniline 4-hydroxylase activity with the two high ascorbic acid levels as compared to the lowest supplementation of ascorbic acid whereas after 2 months no significant changes were observed. 6. Kidney microsomal aniline 4-hydroxylase activity was unaffected by changes in ascorbate status.
D000815:Aniline Hydroxylase / Q000378:metabolism*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D005037:Ethylmorphine-N-Demethylase / Q000378:metabolism*; D006168:Guinea Pigs; D007668:Kidney / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*
D000815:Aniline Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; D010089:Oxidoreductases, N-Demethylating; D005037:Ethylmorphine-N-Demethylase; D001205:Ascorbic Acid