title: Energy and protein relations in the broiler chicken. 4. Role of sex, line and substrate on in vitro lipogenesis.
abstract: Experiments were conducted with dwarf (dw) and normal lines of chickens to determine the effect of sex, diet and line on lipogenesis in the 28-day-old chick. The chicks were fed diets containing 12, 18, 23 and 30% protein. In the first experiment, in vitro lipogenesis (incorporation of [2-14C] sodium acetate into hepatic fatty acids) as well as growth from 7 to 28 days of age were determined in males and females of both lines. In the second experiment, only males and females of the dwarf line were fed to determine the relative contribution of acetate and pyruvate to in vitro lipogenesis (incorporation of either [2-14C] sodium acetate or [2-14C] pyruvate into hepatic fatty acids). Chicks of the dwarf line were smaller (P less than .01) than were those of the normal line. Females of both lines were smaller (P less than .01) than males. In vitro lipogenesis was lower (P less than .01) in the dwarf line; however, the rate for both sexes within a given line was equal. An increase in the dietary protein decreased (P less than .05) in vitro lipogenesis in both lines. The use of pyruvate as an in vitro precursor indicated that the regulation of lipid and carbohydrate metabolism may be an integrated process involving pyruvate carboxylation and subsequent flux of pyruvate carbon into either glucose or fatty acids. Based on the data presented, there is no evidence to assume, that the dwarf gene per se influences lipogenesis.
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage*; D004392:Dwarfism / Q000235:genetics / Q000378:metabolism / Q000662:veterinary; D004435:Eating; D004734:Energy Metabolism; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D005260:Female; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology; D008297:Male; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid
chemical_list: D000085:Acetates; D004044:Dietary Proteins; D005227:Fatty Acids; D008055:Lipids; D011773:Pyruvates; D019289:Pyruvic Acid; D064429:Fatty Acid Synthases; D019342:Acetic Acid