Analysis of the antidepressant phenelzine in brain tissue and urine using electron-capture gas chromatography.
A novel gas chromatographic method for the analysis of the antidepressant phenelzine has been developed. The procedure involves extraction with a liquid ion-pairing compound, back-extraction with HCl, basification, and derivatization with pentafluorobenzoyl chloride. The dipentafluorobenzoyl derivative (structure confirmed by mass spectrometry) is separated and analyzed on a gas chromatograph equipped with a fused silica capillary column and an electron-capture detector. The procedure has been utilized for quantitation of phenelzine in brain of phenelzine-treated rats and in urine from human subjects receiving phenelzine.
D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D010624:Phenelzine / Q000032:analysis* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D010624:Phenelzine