Topological distribution of aminophospholipid fatty acids in trout intestinal brush-border membrane.
The transbilayer distribution of aminophospholipids in trout intestinal brush-border membrane has been investigated using trinitrobenzene sulfonic acid (TNBS). In the middle intestine, phosphatidylethanolamine (PE) is symmetrically distributed between the two leaflets while 68% of the phosphatidylserine (PS) are located in the inner membrane leaflet. In the posterior intestine, 64% of the PE and 69% of the PS are located in the inner membrane leaflet. When asymmetrically distributed, the inner species of PE and PS have a higher content of 22:6(n-3) than the outer ones. This asymmetric distribution of docosahexaenoic acid in trout intestinal brush-border membrane might be related to the rod-like shape of the microvillus membrane and to its metabolism to hydroxylated derivatives.
D000818:Animals; D005227:Fatty Acids / Q000032:analysis*; D007422:Intestines / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis*; D008871:Microvilli / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D014337:Trout
D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids