Kinetic behavior of 30S rRNA intermediate labeled in developing embryonic cells of Xenopus laevis.
In Xenopus neurula cells, "30S" RNA was found to be labeled with 3H-uridine after a relatively short labeling period. Results obtained from cumulative labeling and pulse-labeling and chase experiments with cells from late gastrulae, yolk plug-stage embryos, and neurulae showed that the 30S RNA is an intermediate in rRNA processing and is derived from 40S pre-rRNA and processed to 28S rRNA. The half-life of the 30S rRNA intermediate was about 7.5 min or less at the three stages examined.
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D005775:Gastrula / Q000378:metabolism; D007700:Kinetics; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014982:Xenopus laevis
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C040469:ribosomal protein S30; D014316:Tritium; D014529:Uridine