Mitoxantrone sensitivity of human hematopoietic cell lines.
A panel of 18 human hematopoietic cell lines was tested for sensitivity to mitoxantrone, an anthracenedione derivative with antitumor activity in vitro and in vivo. Cells in logarithmic growth phase were tested for mitoxantrone at concentrations of 0.1-1000 ng/ml for 1-3 days. Cell growth inhibition was profound for all lines at a mitoxantrone concentration of 10 ng/ml, whereas a variable response was seen at a concentration of 1 ng/ml. Inhibition of cell proliferation was mainly depending on arrest at the G2 phase of the cell cycle (or S + G2 at higher mitoxantrone concentrations), together with a cytotoxic effect. The cell cycle block was found in all lines tested, whereas the cytotoxic activity varied much between different cell lines.
D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D008942:Mitoxantrone / Q000494:pharmacology*
D004247:DNA; D008942:Mitoxantrone