Biosynthesis of apolipophorin-III by the fat body in locusts.
Biosynthesis of locust apolipophorin-III (apo-III) was studied in vitro. Gel electrophoresis and immunoblotting analyses of the locust hemolymph demonstrated that apo-III first appears in the hemolymph on the day 3 of the adult stage after the final molt and its hemolymph concentration increases thereafter. When incubated in vitro in a medium containing radioactive amino acid, the fat body cells synthesized the radiolabeled apo-III and released it into the medium. The developmental change in the apo-III synthesizing activity in the fat body reflected that of the apo-III concentration in the hemolymph. RNA isolated from the adult fat body directed the synthesis of apo-III as a major translation product in a cell-free system. These results indicate that the fat body is the tissue responsible for the synthesis of the locust apo-III, and biosynthesis of apo-III is developmentally regulated at the level of mRNA in accordance with the flight activity of the locust.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001053:Apolipoproteins / Q000096:biosynthesis*; D001798:Blood Proteins / Q000378:metabolism; D002474:Cell-Free System; D005216:Fat Body / Q000378:metabolism*; D006110:Grasshoppers / Q000254:growth & development / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification
D001053:Apolipoproteins; D001798:Blood Proteins; D012333:RNA, Messenger; C042470:apolipophorin III