Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants.
The frequencies of non-recombinants are directly determined in 21 human DNA libraries prepared from flow-purified chromosomes. A radiolabeled 18-base oligodeoxynucleotide, whose sequence spans the cloning site, stably hybridizes to non-recombinants. When the cloning site is interrupted by an insert, less stable hybridization occurs. Non-recombinant frequencies range from 1-34% with the frequency of false positives being less than 1%. Libraries are being prepared for each human chromosome as part of the National Laboratory Gene Library Project and the specifications of seven new libraries are presented.
D001483:Base Sequence; D002877:Chromosomes, Human; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D006801:Humans; D007256:Information Systems; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D004247:DNA