A comparison between 45Ca2+ and atomic absorption in calcium flux determinations in perfused rat liver.
Calcium efflux from perfused rat liver following the administration of Ca2+ releasing agents was measured with two methods, 45Ca2+ labeling and atomic absorption. The values obtained with atomic absorption were usually higher than the values obtained with 45Ca2+. These indicate that the intracellular Ca2+ did not equilibrate with the perfusate ca2+ during the 90-minute labeling period. A similar conclusion was reached by measuring the liver 45Ca2+ and 40Ca2+ content. In addition, the types of albumin added to the perfusate influenced the amounts of Ca2+ released.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D008099:Liver / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine; D013054:Spectrophotometry, Atomic
D002132:Calcium Radioisotopes; D012710:Serum Albumin, Bovine; D002118:Calcium