Automatic analysis of G-banded human chromosomes by fast scanning microscope photometry. 2. Practical applications.
The practical applications and improvements to a fast scanning microscope photometer system are described. The production of a density profile is demonstrated which, when taken in conjunction with the chromosome scan, allows more information to be extracted from the data than was previously possible. This enables a more accurate correlation to be made between phenotype and karyotype. The system is demonstrated on preparations of known cytogenetically abnormal individuals, showing the range of problems which can be tackled.
D000328:Adult; D002675:Child, Preschool; D002869:Chromosome Aberrations / Q000175:diagnosis*; D002872:Chromosome Deletion; D025063:Chromosome Disorders; D002879:Chromosomes, Human, Pair 10 / Q000032:analysis; D002889:Chromosomes, Human, Pair 2 / Q000032:analysis; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008853:Microscopy / Q000379:methods; D010783:Photometry / Q000379:methods*; D014178:Translocation, Genetic