Use of resealed erythrocytes as delivery system for C-reactive protein (CRP) to generate macrophage-mediated tumoricidal activity.
We have shown previously in several mouse tumor systems that multilamellar vesicles (MLV) are an effective delivery system for generation of macrophage-mediated tumoricidal activity by C-reactive protein (CRP). Here we show that resealed erythrocyte ghosts (red cell ghosts, RCG) can function in the same manner. CRP associated with red cell ghosts (CRP-RCG) inhibited established lung metastases of T241 fibrosarcoma in C57B1/6J mice. The degree of inhibition was comparable to that observed with CRP-MLV. In other experiments, peritoneal exudate cells, obtained from mice pretreated with CRP-RCG i.p., inhibited growth and pulmonary metastases of T241 tumor in the Winn neutralization assay. Similar results were obtained with MCA-38 colon carcinoma in the Winn assay. These studies indicate that erythrocytes deserve consideration as another delivery system for biological response modifiers.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D002097:C-Reactive Protein / Q000008:administration & dosage*; D003110:Colonic Neoplasms / Q000628:therapy; D004910:Erythrocyte Membrane; D005354:Fibrosarcoma / Q000628:therapy; D066298:In Vitro Techniques; D008081:Liposomes; D008175:Lung Neoplasms / Q000556:secondary / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D009500:Neutralization Tests
D000276:Adjuvants, Immunologic; D008081:Liposomes; D002097:C-Reactive Protein