[Cloning and characteristics of DNA sequences amplified in Djungarian hamster cells with multidrug resistance].
A number of DNA clones containing the amplified DNA sequences were isolated from the genomic library of multidrug-resistant (MDR) Djungarian hamster cells using the DNAC0t 10-250 hybridization probe. Five independent nonoverlapping clones were obtained that covered more than 100 kb of the amplified genomic region. These clones were used as hybridization probes in blot-hybridization with DNA from 7 independently derived MDR Djungarian hamster cell lines selected for the resistance to colchicine or actinomycin D. Some clones contained the DNA sequences amplified in all of the cell lines tested while the others contained the cell line specific amplified sequences. Hybridization in situ was used to localize the amplified DNA in metaphase chromosomes of a MDR cell line that contained about 140 copies of these sequences. The approximate size of an amplicon calculated on the basis of the obtained data is about 1-2 X 10(3) kb.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D005784:Gene Amplification; D009693:Nucleic Acid Hybridization
D004247:DNA
Klonirovanie i kharakteristika uchastkov DNK, amplifitsirovannykh v kletkakh dzhungarskogo khomiachka, obladaiushchikh mnozhestvennoĭ ustoĭchivost'iu.