The use of microinjected colloidal gold and immunocytochemistry to localise pressor sites in the rostral medulla oblongata of the rat.
A new technique for localising the centre of sites of microinjection of drugs in the brain using the silver intensification of colloidal gold is described. The technique is compatible with immunocytochemistry and in this study was used in Wistar-Kyoto rats to investigate the relationship between the location of phenylethanolamine-N-methyltransferase-like immunoreactive (PNMT-LI) cell bodies in the medulla and the precise sites where microinjection of L-glutamate elicited pressor responses. Colloidal gold provided a simple technique for localising the centre of sites of microinjection with a high degree of spatial resolution and allowed pressor sites to be precisely localised in the region of ventrolateral medulla with the highest density of PNMT-LI cells.
D000818:Animals; D006046:Gold / Q000008:administration & dosage*; D006651:Histocytochemistry; D008526:Medulla Oblongata / Q000166:cytology*; D008845:Microinjections; D051381:Rats; D011921:Rats, Inbred WKY
D006046:Gold