Occurrence of pahutoxin and homopahutoxin in the mucus secretion of the Japanese boxfish.
The mucus secreted by the Japanese boxfish O. immaculatus was extracted with n-butanol. The extract was fractionated by silica gel open column chromatography and reversed phase high performance liquid chromatography to give two hemolytic components. The major hemolysin was identical with pahutoxin which was previously isolated from the Hawaiian boxfish O. lentiginosus, while the other hemolysin was a new choline chloride ester, named homopahutoxin.
D000818:Animals; D002794:Choline / Q000031:analogs & derivatives* / Q000032:analysis / Q000633:toxicity; D005397:Fish Venoms / Q000032:analysis* / Q000633:toxicity; D005399:Fishes / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000032:analysis* / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D009093:Mucus / Q000032:analysis*; D011817:Rabbits
D005397:Fish Venoms; D006460:Hemolysin Proteins; C052963:homopahutoxin; C052962:pahutoxin; D002794:Choline