Improved resolution of DNA fragments in polysaccharide-supplemented agarose gels.
The electrophoretic separation of nucleic acids, including small DNA fragments in the range 50-1000 bp, is presently carried out in polyacrylamide gels or in gels containing high concentrations of agarose. We have developed an alternative gel matrix composition which is inexpensive, nontoxic, easy to prepare, and highly transparent to visible and uv light. The composition combines a soluble nonionic polysaccharide such as hydroxyethylcellulose, methylcellulose, or galactomannan with a minimum but sufficient concentration of agarose to form a gel which immobilizes the "liquid phase sieve." These mixtures do not replace polyacrylamide for resolving fragments smaller than approximately 75 nucleotides. However, the new gels show DNA fragment resolution (band separation versus distance traveled) and optical clarity superior to those of conventional agarose.
D002241:Carbohydrates; D002482:Cellulose / Q000031:analogs & derivatives; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005690:Galactose / Q000031:analogs & derivatives; D008351:Mannans; D008747:Methylcellulose; D011108:Polymers
D002241:Carbohydrates; D008351:Mannans; D011108:Polymers; C012990:galactomannan; D002482:Cellulose; C002283:hydroxyethylcellulose; D008747:Methylcellulose; D004247:DNA; D005690:Galactose