Effect of growth temperature on lipid composition and ultraviolet sensitivity of human cells.
Human skin fibroblasts were incubated at either 25 or 37 degrees C before UV irradiation. Cells incubated at 25 degrees C were more resistant to near UV radiation than cells grown at 37 degrees C, but cells grown at the lower temperature were more sensitive to 254 nm radiation. Fatty acid analysis of membranes of cells showed that cells incubated at the lower temperature contained significantly higher amounts of linoleic acid (18:2) and linolenic acid (18:3) than cells incubated at 37 degrees C. To determine if this difference in fatty acid content of the membranes was responsible for the UV survival characteristics of cells incubated at different temperatures, cells were enriched with either linoleate or linolenate during a 37 degrees C incubation period. Gas chromatography revealed that cells incorporated the supplied fatty acid. Fatty acid enriched cells were then irradiated with near UV, and survival characteristics were compared to those obtained with cells grown at the lower incubation temperature. The results suggest that the different proportion of fatty acid content of the cells is not the cause of different UV sensitivities of cells grown at 25 degrees C compared to cells grown at 37 degrees C.
D002455:Cell Division; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000528:radiation effects*; D012867:Skin / Q000166:cytology / Q000528:radiation effects*; D013696:Temperature; D014466:Ultraviolet Rays
D008055:Lipids