[The source and significance of increased urinary dopamine excretion during sodium loading in rats].
To investigate the source and physiological significance of urinary free dopamine (f-DA) in high sodium diet (8% NaCl) loading, the effects of renal denervation and administration of carbidopa, an inhibitor of peripheral dopa decarboxylase, on urinary f-DA excretion were studied in male Wistar rats. Carbidopa was orally administered in a dose of 20mg/kg every 12h. To ascertain the extent of denervation, kidney norepinephrine was measured. Catecholamines were assayed by HPLC with electrochemical detection. Urinary f-DA and sodium excretion were significantly increased in response to the added NaCl. There were few effects of renal denervation on urinary excretion of f-DA and sodium in high sodium diet loading, whereas carbidopa caused a significant fall not only in urinary f-DA excretion but also in urinary sodium excretion. A highly significant correlation was also found between sodium excretion and f-DA excretion on the 5th day of carbidopa treatment. The data indicates that urinary f-DA in a high sodium diet is mainly derived from the renal tubular cells but not from renal nerves and that f-DA may be an important natriuretic hormone.
D000284:Administration, Oral; D000818:Animals; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D002230:Carbidopa / Q000494:pharmacology; D003714:Denervation; D004298:Dopamine / Q000652:urine*; D004837:Epinephrine / Q000378:metabolism; D007668:Kidney / Q000294:innervation / Q000378:metabolism; D008297:Male; D009318:Natriuresis; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000008:administration & dosage*
D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D012965:Sodium Chloride; D002230:Carbidopa; D004298:Dopamine; D004837:Epinephrine