Application of an in vivo culture system for rapid demonstration of anti-Leishmania activity of monoclonal antibodies.
In mice, infection with Leishmania by the subcutaneous route becomes evident after about 2 months. This delay impedes the selection of monoclonal antibodies able to interfere with the infectiousness of the parasite. Using an in vivo culture system--intraperitoneal injection of TG 180 sarcoma cells along with promastigotes or amastigotes--it was possible to define within 15 to 20 days a monoclonal antibody preventing the development of Leishmania. Pretreatment of promastigotes and amastigotes of several Leishmania species with a monoclonal antibody raised against Leishmania infantum prevented infection equally in either system. These cross-reactions may be of importance in designing new approaches of immunotherapy.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002478:Cells, Cultured; D005260:Female; D007891:Leishmania / Q000276:immunology*; D007893:Leishmania donovani / Q000276:immunology; D007896:Leishmaniasis / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors
D000911:Antibodies, Monoclonal