Interaction of seminalplasmin with chlortetracycline, a fluorescent chelate probe of Ca2+.
The interaction of seminalplasmin with chlortetracycline, a fluorescent chelate probe of Ca2+, was studied. The results indicate that seminalplasmin binds to chlortetracycline. The binding is not influenced by salt. Both Ca2+ and seminalplasmin probably bind to the same site on chlortetracycline. Seminalplasmin also reduced the Tb3+-associated fluorescence of bovine spermatozoal plasma membrane. These results are discussed in relation to the inhibitory effect of seminalplasmin on the uptake of Ca2+ in bovine spermatozoa.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002751:Chlortetracycline / Q000378:metabolism*; D003864:Depression, Chemical; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D029607:Seminal Vesicle Secretory Proteins; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013725:Terbium / Q000494:pharmacology
D005456:Fluorescent Dyes; D011506:Proteins; D029607:Seminal Vesicle Secretory Proteins; D013725:Terbium; D002118:Calcium; D002751:Chlortetracycline