The transcription of B2 repeated sequences is regulated during the transition from quiescent to proliferative state in cultured rodent cells.
The RNA polymerase III-dependent transcription of B2 repeated sequences has been monitored during the transition from the quiescent to proliferative state in cultured rodent cells and after polyomavirus-induced transformation. The level of RNAs containing B2 sequences was found to be higher in both the proliferative state of normal cells and in polyomavirus-transformed cells. In both systems, nuclear run-off transcription assays indicated that high levels of B2 RNAs are due to an enhanced transcription rate. These results suggest the presence of a B2-specific RNA pol III transcription factor(s) whose activity is sensitive to cell cycle progression and oncogenic transformation.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004261:DNA Replication; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D012320:RNA Polymerase III / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D007532:Isoleucine; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III