Bromcresol purple dye-binding methods underestimate albumin that is carrying covalently bound bilirubin.
We used automated and manual versions of two commonly used dye-binding methods for albumin to quantify albumin that had been modified by covalent addition of bilirubin. Dye-binding methods based on reaction with bromcresol purple underestimated the modified albumin by as much as 29%. Dye-binding methods based on reaction with bromcresol green were unaffected by the presence of covalently attached bilirubin. Neither method was affected by the presence of noncovalently bound bilirubin.
D001663:Bilirubin / Q000378:metabolism*; D001961:Bromcresol Green; D001962:Bromcresol Purple; D003408:Cresols; D006801:Humans; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis* / Q000378:metabolism
D003408:Cresols; D012709:Serum Albumin; D001962:Bromcresol Purple; D001961:Bromcresol Green; D001663:Bilirubin