The synthesis of stereoselectively labelled porphobilinogen and its incorporation into protoporphyrin-IX.
11-(R)-2H porphobilinogen, stereospecifically labelled with deuterium in the aminomethylene group has been incorporated into protoporphyrin-IX by haemolysates of chicken erythrocytes. High field NMR spectroscopy confirms that the overall biochemical process is stereospecific, deuterium being retained at the alpha-, gamma- and delta-meso positions and lost from the beta-meso position.
D000818:Animals; D002645:Chickens; D003903:Deuterium; D004912:Erythrocytes / Q000378:metabolism*; D008968:Molecular Conformation; D011162:Porphobilinogen / Q000097:blood / Q000138:chemical synthesis*; D011166:Porphyrins / Q000097:blood*; D011524:Protoporphyrins / Q000097:blood*
D011166:Porphyrins; D011524:Protoporphyrins; D011162:Porphobilinogen; D003903:Deuterium; C028025:protoporphyrin IX