[Assay of thermolabile enterotoxins of Escherichia coli and Vibrio cholerae by inhibition of erythroadsorption on the GM1 ganglioside].
A GM1 erythroassay (GERYDO) for heat-labile Escherichia coli enterotoxin (LT) and cholera toxin (CT) is described. This assay was developed for use in poorly equipped laboratories in developing countries. It uses GM1-coated polystyrene plates and is based on the competition between the toxin to be assayed and CT covalently bound to sheep red blood cells. GERYDO can detect 0.9 or 0.5 ng of CT per ml depending on the method of sensitization of erythrocytes. Good quantitative and qualitative correlation with the enzyme-linked immunosorbent assay and the Vero cell test was observed.
D001427:Bacterial Toxins / Q000032:analysis*; D004768:Enterotoxins / Q000032:analysis*; D029968:Escherichia coli Proteins; D005069:Evaluation Studies as Topic; D005677:G(M1) Ganglioside / Q000378:metabolism*; D006383:Hemadsorption Inhibition Tests / Q000379:methods*; D013696:Temperature
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C037420:stN protein, Vibrio cholerae; D005677:G(M1) Ganglioside; C027538:heat-labile enterotoxin, E coli
Dosage de l'entérotoxine thermolabile de Escherichia coli et de Vibro cholerae par inhibition de l'érythro-adsorption sur le ganglioside GM1.