Thyroid hormone affects the expression of neoplastic transformation induced by DNA-transfection.
Thyroid hormone can dramatically modulate oncogenic transformation of cells in culture. To further investigate this we have used DNA-mediated gene transfer (transfection) to transform cells grown in the presence (+T3) or absence (-T3) of thyroid hormones. Removal of thyroid hormones from the culture media greatly reduced the appearance of transformed foci subsequent to transfection. However, +T3 or -T3 media had no effect on the appearance of ouabain-resistant (ouar) colonies following transfection of ouabain-sensitive (ouas) cells with DNA isolated from ouar cells and selection in 3 mM ouabain. These results suggest that thyroid hormone does not effect the uptake or integration of exogenous DNA, but instead may modify the expression of transformation.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000209:etiology* / Q000648:ultrastructure; D003470:Culture Media; D004247:DNA / Q000302:isolation & purification; D004351:Drug Resistance; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D009154:Mutation; D009857:Oncogenes / Q000187:drug effects*; D010042:Ouabain / Q000494:pharmacology; D013963:Thyroid Hormones / Q000494:pharmacology*; D014162:Transfection
D003470:Culture Media; D013963:Thyroid Hormones; D010042:Ouabain; D004247:DNA