Interrelationships among X-ray-induced anchorage independence, mutagenesis and chromosomal rearrangements in human diploid fibroblasts.
The changes in the frequencies of X-ray-induced mutations to 6-thioguanine resistance, chromosomal abnormalities and transformation to anchorage-independent growth were examined during confluent holding recovery in density-inhibited cultures of human diploid fibroblasts. Chromosomal abnormalities studied included non-stable aberrations, deletions, and reciprocal translocations measured up to 20 mean population doublings post-irradiation. Complex recovery kinetics were observed. A comparison of these kinetics suggests that reciprocal translocations are associated with the induction of both transformation and mutations. Mutagenesis correlated with deletions and translocations, whereas transformation correlated with translocations only.
D002448:Cell Adhesion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D004351:Drug Resistance; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D007621:Karyotyping; D009154:Mutation; D013866:Thioguanine / Q000494:pharmacology
D013866:Thioguanine