Rapid method to prepare mammalian oocytes and embryos for transmission electron microscopy.
A method for the preparation of mammalian oocytes and embryos for transmission electron microscopy employing agar embedding of oocytes and re-embedding of semithin sections is described. This method offers rapid and safe handling of small specimens, and it allows analyses of even very small structures in the oocytes.
D000818:Animals; D002417:Cattle; D005404:Fixatives; D008322:Mammals; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy / Q000379:methods; D009865:Oocytes / Q000648:ultrastructure*; D015053:Zygote / Q000648:ultrastructure*
D005404:Fixatives