An enzyme-linked immunosorbent assay for detection of anti-Bacillus piliformis serum antibody in rabbits.
An enzyme-linked immunosorbent assay (ELISA) is described for the detection of rabbit serum antibody directed against the causative agent of Tyzzer's disease, Bacillus piliformis. Ninety-four percent agreement was found between the ELISA and an indirect fluorescent antibody test. The sensitivity of the ELISA was 95% and its specificity was 92% as compared to the indirect fluorescent antibody test (IFAT). The rabbit origin B. piliformis isolate used in this ELISA was found to be cross-reactive by ELISA and IFAT to B. piliformis isolates of rat, gerbil and horse origin. This suggests that a single B. piliformis isolate may be used as antigen for an ELISA utilizable for multiple species.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001407:Bacillus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D011817:Rabbits
D000907:Antibodies, Bacterial