A retinal whole-mount method useful in detecting retrogradely-labeled ganglion cells.
The present report describes a simple retinal whole-mount method that is compatible with horseradish peroxidase histochemistry. The retina is removed unfixed, flattened and fixed. Histochemical procedures are carried out with the retina free-floating, prior to affixing it to the slide with formaldehyde vapor. The method consistently yields whole-mounts free of pigment epithelium and firmly affixed to the slide, with good preservation of retinal morphology and surface topography.
D000818:Animals; D006224:Cricetinae; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase; D008647:Mesocricetus; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology*
D006735:Horseradish Peroxidase