Comparison of the protective effects by human and bovine superoxide dismutase against ischemia- and reperfusion-induced impairment of kidney function in anesthetized rats.
In a rat model of ischemia- and reperfusion-induced kidney damage, the protective effects of human superoxide dismutase produced by genetic technology (hum-SOD) were compared to those of bovine superoxide dismutase (bov-SOD). The intravenous infusion of hum-SOD and bov-SOD, started concomitantly with the kidney reperfusion after a 60-min (or 30-min) period of ischemia, significantly improved the renal function (inulin and p-amino-hippuric acid clearance rates) as compared to the vehicle-treated control group. In contrast, inactive apoenzyme of superoxide dismutase (Apo-SOD) did not improve the impaired renal function after the kidney reperfusion. Therefore, the kidney protection by hum-SOD and bov-SOD may reasonably be ascribed to their specific enzymatic function--scavenging of oxygen radicals. In this respect, hum-SOD proved to be as effective as bov-SOD. To our knowledge this is the first report on a direct pharmacologic comparison of superoxide dismutases from natural and recombinant origin.
D000818:Animals; D001051:Apoenzymes / Q000494:pharmacology; D002417:Cattle; D005609:Free Radicals; D006801:Humans; D007444:Inulin / Q000378:metabolism; D007511:Ischemia / Q000188:drug therapy* / Q000378:metabolism; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000494:pharmacology*; D010130:p-Aminohippuric Acid / Q000378:metabolism
D001051:Apoenzymes; D005609:Free Radicals; D007444:Inulin; D013482:Superoxide Dismutase; D010100:Oxygen; D010130:p-Aminohippuric Acid