title: Determination of apolipoprotein variants by isoelectric focusing in agarose.
abstract: Isoelectric focusing (IEF) of apolipoproteins is an important procedure for the isolation of apolipoprotein isoforms. In this paper we describe the use of agarose/urea as a focusing medium for IEF of apolipoproteins. This technique, which offers several advantages over the traditional polyacrylamide gel focusing, yields a further microheterogeneity of the known isoforms of apolipoproteins A-I and E. In addition, by subsequent immunoprecipitation, both specificity and sensitivity are enhanced. Using a monospecific antiserum against Apo A-I, a new minor isoform was detected at the basic end of the Apo A-I spectrum (pI 6.02) which has not yet been observed in normal plasma samples. Another advantage of this technique in combination with immunoprecipitation is the specific detection of Apo E phenotypes which is essential for the diagnosis of hyperlipoproteinemia type III.
mesh_terms: D001053:Apolipoproteins / Q000097:blood*; D011232:Chemical Precipitation; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007120:Immunochemistry; D007525:Isoelectric Focusing / Q000379:methods; D010641:Phenotype; D014238:Trichloroacetic Acid; D014508:Urea
chemical_list: D001053:Apolipoproteins; D014238:Trichloroacetic Acid; D014508:Urea