title: A glyco-phosphoprotein in human milk.
abstract: A highly glycosylated phosphoprotein (HGPP) was isolated from a human casein fraction by reversed-phase high-performance liquid chromatography. This component contained carbohydrates to approximately 38.2% (w/w) and phosphorus to approximately 1.6% (w/w). The molecular weight of this HGPP as estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis approximately 41,000. Ultracentrifugal analysis revealed that the sedimentation coefficient of the HGPP was 2.6S in a buffer at pH 7.0 and 27 degrees C, but this component interacted with human k-casein and formed a complex with s = 10.4S.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008895:Milk, Human / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D006023:Glycoproteins; D010750:Phosphoproteins