Immuno-enhancing activity of the amino-terminal domain of human prealbumin: isolation, characterization and synthesis.
A decapeptide isolated from highly purified preparations of human prealbumin was able to restore azathioprine (Az) sensitivity, a property of a sub-class of T-lymphocytes, to the spleen rosette-forming cells (RFC) of adult thymectomized (ATx) mice in vitro. The peptide was sequenced by the Edman method and shown to correspond to the ten amino-terminal residues of prealbumin, Gly-Pro-Thr-Gly-Thr-Gly-Glu-Ser-Lys-Cys. Synthesis of this peptide by solid phase methodology confirmed its activity both in vitro and in vivo. Synthesis of a number of structural analogues indicated that the amino-terminal deca, undeca and dodecapeptides of prealbumin as well as some of their derivatives were also able to restore Az sensitivity to RFC in vitro and in vivo. The Cys10 residue and the Glu7 residues both contributed significantly to potency in vitro. Removal of up to three amino acids from the N-terminus of the decapeptide led to a progressive loss of activity. The data indicates that the ability of human prealbumin to restore the Az sensitivity to the RFC of adult Tx mice is intrinsic to the protein and resides in the amino-terminal domain of the molecule.
D000595:Amino Acid Sequence; D000818:Animals; D001379:Azathioprine / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology; D011228:Prealbumin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D012397:Rosette Formation; D013154:Spleen / Q000187:drug effects / Q000276:immunology*
D010446:Peptide Fragments; D011228:Prealbumin; D001379:Azathioprine