The application of 1H/13C inversely correlated NMR spectroscopy to the determination of acylation and glycosylation sites in the O-specific polysaccharide from Hafnia alvei 1187.
An inversely correlated 1H/13C NMR spectrum defined the amino sugars acylated by acetyl or 3-hydroxybutyryl groups and revealed partial sequences and glycosylation sites in a tetrasaccharide repeating unit of the title polysaccharide, (----2DGlc alpha 1----3DGlcNAcyl alpha 1----4DGalNAc alpha 1----3DGalNAc beta 1----)n, where Acyl = 3-hydroxybutyryl.
D000215:Acylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D004755:Enterobacteriaceae / Q000276:immunology*; D006027:Glycosides; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011135:Polysaccharides, Bacterial
D002247:Carbon Isotopes; D006027:Glycosides; D011135:Polysaccharides, Bacterial; D006859:Hydrogen