Antimicrobial activity of ceftriaxone, cefotaxime, desacetylcefotaxime, and cefotaxime-desacetylcefotaxime in the presence of human serum.
Eighty-seven organisms were tested against ceftriaxone, cefotaxime, desacetylcefotaxime, and the combination of cefotaxime and desacetylcefotaxime (1:1 ratio) in broth containing 0, 25, or 50% human serum. In the presence of human serum, ceftriaxone MICs were four- to eightfold higher than those obtained in broth, changing 98% of Staphylococcus aureus strains from the susceptible to the moderately susceptible category and 53% of selected gram-negative strains to a more resistant category. The MICs of cefotaxime, desacetylcefotaxime, and cefotaxime plus desacetylcefotaxime were not adversely affected by human serum; in fact, the bactericidal activity was slightly improved.
D001419:Bacteria / Q000187:drug effects*; D001798:Blood Proteins / Q000378:metabolism* / Q000494:pharmacology; D002439:Cefotaxime / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D002443:Ceftriaxone / Q000378:metabolism / Q000494:pharmacology*; D004338:Drug Combinations; D004352:Drug Resistance, Microbial; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000187:drug effects
D001798:Blood Proteins; D004338:Drug Combinations; C029281:desacetylcefotaxime; D002443:Ceftriaxone; D002439:Cefotaxime