Differences in the rotation spectra of mouse oocytes and zygotes.
Rotation spectra of mouse oocytes, zygotes and embryos in the two-cell stage under the influence of high-frequency rotating fields were studied. The characteristic frequency (fc1) of cells isolated from superovulated + mated mice is different from that of oocytes. This was attributed to an increase in the membrane resistance and, less probably, to a change in the zona pellucida conductivity. The rotation spectra can be used to differentiate between non-fertilized and fertilized eggs. A theoretical interpretation of the measured spectra and simulation of the changes caused by fertilization is given.
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D004558:Electric Stimulation; D004594:Electrophysiology; D005260:Female; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D012399:Rotation; D015044:Zona Pellucida / Q000502:physiology; D015053:Zygote / Q000502:physiology*