Studies on tumor necrosis factor (TNF)--I. Pharmacokinetics of human recombinant TNF in rabbits and monkeys after intravenous administration.
By intravenous (bolus) administration of human recombinant tumor necrosis factor (2.5 X 10(6) units) into rabbits and monkeys it has been possible to follow its decay in the plasma and to determine the pharmacokinetic parameters. After labelling TNF with 125I, simultaneous determination of protein-bound radioactivity and bioactivity was carried out with the result that radioactivity decays somewhat slower than bio-activity suggesting that the use of the former tracer alone may underestimate TNF catabolism. Simultaneous determination of 125I-TNF in rabbit plasma and lymph after intravenous and subcutaneous administration indicated a lymph to plasma TNF concentration ratio of about 0.1 and 1 or more for the two routes, respectively.
D000818:Animals; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes; D007700:Kinetics; D008196:Lymph / Q000378:metabolism; D008252:Macaca fascicularis; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha