Nucleic acid chemistry: resolution of supercoiled, intact relaxed, and nicked double-stranded DNAs by formamide-sucrose gradients.
A technique which will separate superhelical, intact nonsuperhelical, and damaged mitochondrial DNA molecules by use of sedimentation gradients of formamide-sucrose is described. This technique can be used to detect damage to the mitochondrial DNA helix.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004270:DNA, Circular / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis*; D004278:DNA, Superhelical / Q000032:analysis; D005260:Female; D005559:Formamides; D008930:Mitochondria, Liver / Q000032:analysis; D009690:Nucleic Acid Conformation; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose
D004270:DNA, Circular; D004272:DNA, Mitochondrial; D004278:DNA, Superhelical; D005559:Formamides; D013395:Sucrose