title: Inhibition of phosphoglucose isomerase allozymes from the wing polymorphic waterstrider, Limnoporus canaliculatus, by pentose shunt metabolites.
abstract: Inhibition of phosphoglucose isomerase (PGI) allozymes from the wing-polymorphic waterstrider, Limnoporus canaliculatus, by three pentose-shunt metabolites was studied at several different temperatures. This was done to determine if the allozymes exhibited a differential ability to participate in lipid biosynthesis via differential partitioning of carbon flux through the pentose shunt versus glycolysis. 6-Phosphogluconate and erythrose-4-phosphate proved to be strong competitive inhibitors of PGI, while sedoheptulose-7-phosphate was a very weak inhibitor. The PGI allozymes from L. canaliculatus were differentially inhibited by 6-phosphogluconate at two of the three temperatures studied. However, this property does not appear to be an adaptive difference between the allozymes but, rather, a correlated effect resulting from variation in substrate binding. Estimates of reaction rates for the allozymes indicate that the differences in inhibition result in no detectable differences in reaction velocities. Thus, no evidence in support of the hypothesis that PGI allozymes from Limnoporus canaliculatus were adapted to function in different metabolic capacities via differential inhibition was obtained in this study. However, the importance of this characteristic in allozymic adaptation in natural populations remains an open question.
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D005942:Gluconates / Q000494:pharmacology; D005956:Glucose-6-Phosphate Isomerase / Q000037:antagonists & inhibitors* / Q000235:genetics; D006430:Hemiptera / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D010427:Pentose Phosphate Pathway; D011110:Polymorphism, Genetic; D013403:Sugar Phosphates / Q000494:pharmacology
chemical_list: D005942:Gluconates; D013403:Sugar Phosphates; C020495:sedoheptulose 7-phosphate; C026959:erythrose 4-phosphate; D005956:Glucose-6-Phosphate Isomerase; C008884:6-phosphogluconic acid