title: Protein C: an automated activity assay.
abstract: Protein C in citrated plasma is found to be specifically activated by the snake venom derivative Protac, and the activator is used in a simple, automated assay method. The activation is completed in less than 120 s and an isolation of protein C from its inhibitor before activation is not necessary. The activated protein C is determined with the chromogenic substrate S-2366. Therapeutic concentrations of heparin in the test sample do not influence the result. A strong positive correlation to immunoassay of protein C was found (r = 0.92). Three cases of probable hereditary protein C deficiency belonging to the same family were discovered during the study.
mesh_terms: D000328:Adult; D001322:Autoanalysis / Q000379:methods*; D004796:Clinical Enzyme Tests / Q000379:methods; D005069:Evaluation Studies as Topic; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007118:Immunoassay; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000494:pharmacology; D011486:Protein C / Q000032:analysis* / Q000235:genetics; D020151:Protein C Deficiency; D012015:Reference Standards; D013917:Thrombin; D014859:Warfarin / Q000494:pharmacology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011486:Protein C; C049647:snake venom protein C activator; D014859:Warfarin; D006493:Heparin; D013917:Thrombin