Separation and identification of geometric isomers of 3-hydroxyretinoids and occurrence in the eyes of insects.
The 9-cis, 11-cis, 13-cis and all-trans isomers of 3-hydroxyretinal oxime, and the 11-cis, 13-cis and all-trans isomers of 3-hydroxyretinol have been resolved by high pressure liquid chromatography (HPLC) and identified by their spectral properties. The antimony chloride reaction product of 3-hydroxyretinol is spectrally indistinguishable from that of retinol. Heads of insects from 8 genera of Diptera and Lepidoptera were found to contain 3-hydroxyretinoids. The most abundant isomers of 3-hydroxyretinal are 11-cis and all-trans.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004330:Drosophila / Q000378:metabolism*; D005123:Eye / Q000032:analysis*; D007536:Isomerism; D007915:Lepidoptera / Q000378:metabolism*; D012172:Retinaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D012176:Retinoids; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000032:analysis
D012176:Retinoids; D014801:Vitamin A; C015943:3-hydroxyretinol; C049428:3-hydroxyretinal oxime; D012172:Retinaldehyde