The role of microtubules in the phagocytosis of collagen by fibroblasts.
The effects of the anti-microtubular agents, colchicine and vinblastine, on the phagocytosis of collagen by fibroblasts were assessed quantitatively in cultured mouse bone explants. It was found that in the absence of the microtubular system the volume density of lysosomal vacuoles containing cross-banded collagen fibrils in periosteal cells did not differ from that seen in controls. In contrast, cytochalasin B which interferes with the microfilament system prevented the accumulation of collagen-containing vacuoles in the cytoplasm. The data indicate that the phagocytosis of collagen fibrils by fibroblasts does not depend on the integrity of the microtubular apparatus, but seems to require an intact microfilament system.
D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism*; D003571:Cytochalasin B / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D005347:Fibroblasts / Q000378:metabolism; D007976:Leupeptins / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects; D014617:Vacuoles / Q000378:metabolism / Q000648:ultrastructure; D014747:Vinblastine / Q000494:pharmacology
D007976:Leupeptins; D003571:Cytochalasin B; D014747:Vinblastine; D003094:Collagen; C032854:leupeptin; D003078:Colchicine