A general transcription factor forms a stable complex with RNA polymerase B (II).
A general transcription factor (BTF3) has been purified from HeLa whole cell extracts and shown to be required for accurate initiation of transcription from the adenovirus-2 major late promoter (Ad2MLP) and other RNA polymerase class B promoters. We show that purified BTF3 (27 kd) binds to RNA polymerase B (II), forming a complex that is transcriptionally active. We found no evidence that purified BTF3 interacts with DNA or is required for the formation of the stable preinitiation complex.
D000256:Adenoviridae / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic; D014157:Transcription Factors; D014158:Transcription, Genetic
D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases