title: Large scale nonproton ion release and bacteriorhodopsin's state of aggregation in lipid vesicles. I. Monomers.
abstract: Light-induced conductivity transients have been observed in preparations of bacteriorhodopsin (bR) in phospholipid vesicles at high lipid/protein molar ratios. Under these conditions, bR is known to be dissolved as monomers in the lipid bilayer. The conductivity transients are due mostly to proton movements, including a trans-membrane component. Kinetic resolution of the conductance change due to proton ionophore-induced leakage through the vesicle membrane provides a novel method to quantitate the number of protons pumped, even in heavily buffered solutions. Some of the transient signal seen on the timescale of the bR photocycle is due to nonproton ions but is smaller than that observed in native purple membranes at pH 7 in low salt. Furthermore, when the pH is raised to 8, the very large transient nonproton ion release seen in purple membranes is not seen in the vesicles. This correlates well with previous results (Marinetti, T., and D. Mauzerall, 1986, Biophys. J., 50:405-415), in which the nonproton ion movements observed with native purple membranes were abolished by solubilization in Triton X-100. Thus, the nonproton ion release appears to be a property of bR in the native aggregated state.
mesh_terms: D001436:Bacteriorhodopsins / Q000502:physiology*; D004134:Dimyristoylphosphatidylcholine; D004553:Electric Conductivity; D006217:Halobacterium / Q000502:physiology; D008027:Light; D008081:Liposomes; D046911:Macromolecular Substances; D008954:Models, Biological
chemical_list: D008081:Liposomes; D046911:Macromolecular Substances; D001436:Bacteriorhodopsins; D004134:Dimyristoylphosphatidylcholine