Affinity purification of cytosolic epoxide hydrolase from human, rhesus monkey, baboon, rabbit, rat and mouse liver.
An affinity purification system based on elution of cytosolic epoxide hydrolase from a methoxycitronellyl thiol ligand with 4-azidochalcone oxide was applied to a variety of samples including liver from human, monkey, baboon, rabbit, rat and mouse as well as mammary gland from mouse. Hepatic tissues yielded a major 58 kDa band on SDS-PAGE, but the system had to be modified slightly to remove a 33 kDa band for rat. All of the affinity purified hydrolases showed similar properties with regard to substrate selectivity, pH dependence and mobilities on SDS-PAGE.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003600:Cytosol / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004851:Epoxide Hydrolases / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008099:Liver / Q000201:enzymology; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000201:enzymology; D008875:Middle Aged; D010215:Papio; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D004851:Epoxide Hydrolases