title: Effects of dipalmitoylphosphatidylcholine liposomes on high affinity transport of choline and synthesis of acetylcholine in cerebral cortical synaptosomes in the rat.
abstract: The presynaptic cholinergic effects of the phospholipid dipalmitoylphosphatidylcholine were investigated in non-depolarized synaptosomes from the cerebral cortex of the rat. This naturally-occurring phospholipid, in the form of small unilamellar vesicles (3.0 mg/ml), inhibited both high affinity uptake of [3H] choline and the synthesis of [3H] acetylcholine. This lipid-induced inhibition was apparently competitive, since it was observed at 1 microM but not 20 microM extracellular concentration of choline. These results indicate that dipalmitoylphosphatidylcholine may reduce the synthesis of acetylcholine in resting nerve terminals.
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000494:pharmacology*; D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D002794:Choline / Q000378:metabolism*; D003864:Depression, Chemical; D066298:In Vitro Techniques; D008081:Liposomes / Q000008:administration & dosage; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D002794:Choline; D000109:Acetylcholine