Effects of Maillard browned egg albumin on drug-metabolizing enzyme systems in the rat.
Adult male Sprague-Dawley rats were fed a purified diet containing 5% Maillard browned egg albumin (EA-B) or browned hydrolysed egg albumin (HEA-B) for 10 wk. Control animals were pair-fed a corresponding isocaloric, isonitrogenous non-browned egg albumin (EA-C) or hydrolysed egg albumin (HEA-C) diet. At the end of 10 wk, the rats were killed and hepatic, small intestinal and colonic microsomes and cytosol fractions were prepared by ultracentrifugation. Animals fed EA-B exhibited significantly (P less than 0.05) increased hepatic benzo[alpha]pyrene hydroxylase activity and significantly (P less than 0.05) decreased colonic aminopyrine N-demethylase activity compared to control (EA-C) animals. HEA-B-fed animals also exhibited a significant (P less than 0.05) decrease in colonic aminopyrine N-demethylase activity compared with HEA-C controls, but no significant differences were detected in hepatic or small intestinal enzyme activities in this group. These data suggest that Maillard browned protein products may modify hepatic and/or colonic drug-metabolizing enzyme system activities, and may thus contribute to alterations in the metabolism of endogenous substrates and of exogenous drugs, precarcinogens and other xenobiotics.
D000633:Aminopyrine N-Demethylase / Q000032:analysis; D000818:Animals; D001579:Benzopyrene Hydroxylase / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D003106:Colon / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D010047:Ovalbumin / Q000494:pharmacology*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004364:Pharmaceutical Preparations; D010047:Ovalbumin; D001579:Benzopyrene Hydroxylase; D000633:Aminopyrine N-Demethylase