title: The antibiotic W341C, its ion transport properties and inhibitory effects on mitochondrial substrate oxidation.
abstract: We have examined the ion transport properties and the inhibition of rat liver mitochondrial substrate oxidation by the antibiotic W341C. W341C was able to transport 22Na+ and 42K+ across a bulk carbon tetrachloride layer. A preference was shown for K+ transport. With equal molar antibiotic concentrations, W341C transported 42K+ at a greater rate than the K+-selective ionophore nigericin, but transported 22Na+ at a lesser rate than the Na+-selective ionophore monensin. Like nigericin, W341C was able to deplete mitochondrial K+, but not Mg2+ nor Ca2+. The inhibition of mitochondrial substrate oxidation by W341C paralleled the patterns obtained with nigericin. These data indicate that W341C is a K+-selective ionophore that inhibits mitochondrial substrate oxidation by a mechanism analogous to that of nigericin.
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D001149:Arsenates / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002251:Carbon Tetrachloride; D002412:Cations; D004988:Ethers, Cyclic / Q000378:metabolism / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D008985:Monensin / Q000378:metabolism; D009550:Nigericin / Q000378:metabolism / Q000494:pharmacology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001149:Arsenates; D002412:Cations; D004988:Ethers, Cyclic; C053077:W 341C; D008985:Monensin; D012964:Sodium; D002251:Carbon Tetrachloride; D008274:Magnesium; C025657:arsenic acid; D009550:Nigericin; D011188:Potassium; D002118:Calcium