[A study of tumor-associated cellular proteins from carcinoma and adenoma of the human colon].
In order to detect tumor-associated cellular proteins and to obtain information pertaining to the theory of an adenoma-carcinoma sequence, cellular proteins from mucosa, carcinoma, adenoma, carcinoma in adenoma, and polypoid carcinoma of the human colon were analyzed by two-dimensional (isoelectric focusing-SDS PAGE) electrophoresis. The results revealed the presence of about 300 spots with pIs from 5.5 to 8.5 and MWs from 20,000 to 200,000 in all of the five tissue types. The vast majority of them were common to these five tissue types. However, there were nine spots which differed between mucosa and carcinoma of the colon: Three spots (82/6.3, 65/8.2, 56/8.1, MW X 10(-3)/pI) were detected often in carcinoma but seldom in mucosa, and four spots (72/8.2, 72/8.5, 61/7.5, 38/6.5) were increased in amount in carcinoma. These seven spots could be the tumor-associated cellular proteins. The remaining two spots (31/7.2, 28/6.5), which were decreased in amount in carcinoma, were considered to be normal colon-associated cellular proteins. The three spots 82/6.3, 65/8.2 and 56/8.1 were also detected in adenoma, carcinoma in adenoma, and polypoid carcinoma. The four spots 72/8.2, 72/8.5, 61/7.5 and 38/6.5 were increased in amount in these three tissue types and spots 31/7.2 and 28/6.5 were decreased. The above results might provide evidence, though indirect, for the theory of an adenoma-carcinoma sequence.
D000236:Adenoma / Q000032:analysis*; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D002277:Carcinoma / Q000032:analysis*; D003110:Colonic Neoplasms / Q000032:analysis*; D003111:Colonic Polyps / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*
D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins