Routine dot-blot assay of multiple serum samples using a simple apparatus.
This paper describes a simple method for carrying out dot-blot immunoassays simultaneously with a large number of different sera. No dot-blot manifold is necessary, only a modified 96-well flat-bottom microtiter plate. This apparatus is re-usable, inexpensive and especially suited for field testing of patient sera in serodiagnostic assays. One application of this technique, to the serodiagnosis of visceral leishmaniasis on a pure parasite protein, is demonstrated.
D000906:Antibodies / Q000032:analysis*; D000953:Antigens, Protozoan / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D006801:Humans; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D012698:Serologic Tests
D000906:Antibodies; D000953:Antigens, Protozoan