title: Vectorial secretion of transferrin and androgen binding protein in Sertoli cell cultures: effect of extracellular matrix, peritubular myoid cells and medium composition.
abstract: We examined the effect of various culture conditions on the polarized secretion of androgen binding protein (ABP) and transferrin (Trf) by Sertoli cells (Sc) in vitro. Sc from 18-day-old rats were cultured as confluent monolayers on permeable membranes in two-compartment chambers for up to 11 days. Coating of the membranes with extracellular matrix (ECM) components: collagen IV + laminin (CL) or reconstituted basement membrane (RBM) enhanced ABP and Trf secretion (200% and 150%, respectively), with RBM being more effective than CL in stimulating ABP but not Trf secretion. Neither CL nor RBM significantly influenced the relative amounts of ABP and Trf secreted into the outer (OC) and inner (IC) compartments of the culture chamber (OC/IC ratio). All of these effects were not significantly influenced by the presence of testosterone and serum. Co-culture of Sc with peritubular myoid cells (Pc) significantly increased the secretion of both ABP and Trf, although the magnitude of stimulation and the time-response patterns were different for each protein. Co-culture with Pc also dramatically increased the OC/IC ratios for ABP and Trf. Testosterone (10(-6) M) enhanced the Pc effects. In cultures of Sc alone, presence of 2% fetal bovine serum increased the OC/IC ratios, whereas testosterone had no effect. Based on these results, we suggest a possible role of Pc in the regulation of Sc polarized secretions.
mesh_terms: D000727:Androgen-Binding Protein / Q000378:metabolism*; D000818:Animals; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D003470:Culture Media; D005109:Extracellular Matrix / Q000502:physiology*; D007444:Inulin; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000502:physiology*; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013737:Testis / Q000502:physiology*; D013739:Testosterone / Q000494:pharmacology; D014168:Transferrin / Q000378:metabolism*
chemical_list: D000727:Androgen-Binding Protein; D003470:Culture Media; D014168:Transferrin; D013739:Testosterone; D007444:Inulin