Purification of a chick brain-derived growth factor by reversed-phase high-performance liquid chromatography.
A chick brain-derived growth factor (CBGF) has been purified to apparent homogeneity by a combination of ultrafiltration, DEAE ion-exchange, and reversed-phase high-performance liquid chromatography on a C18 column. CBGF has an apparent molecular mass of approximately 1500 Da. The amino acid composition of CBGF has been determined and reveals a peptide containing predominantly glycine, glutamic acid/glutamine, and aspartic acid/asparagine. This mitogen is highly active, with half-maximal stimulation of chick brain astrocytes at 5 ng/ml in an assay using incorporation of methyl-3H-thymidine into DNA. In addition to its effects on chick astrocytes, purified CBGF from the 18 d chick embryo is mitogenic for amphibian limb regeneration blastema cells in vitro. A possible role for this mitogen in the nerve-dependent regeneration of amphibian appendages is discussed.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005121:Extremities / Q000187:drug effects / Q000502:physiology; D006133:Growth Substances / Q000302:isolation & purification*; D008934:Mitogens / Q000302:isolation & purification*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D012038:Regeneration; D012455:Salamandridae / Q000502:physiology*
D006133:Growth Substances; D008934:Mitogens; D009419:Nerve Tissue Proteins; C046909:chicken brain growth factor