Cerebellar metabolism of phosphatidylethanolamine and its water-soluble precursors during bicuculline-induced convulsive seizures.
The incorporation of labeled ethanolamine into phosphatidylethanolamine (PE) and its water-soluble precursors, phosphoethanolamine and CDP-ethanolamine, is measured in rat cerebella during the course of bicuculline-induced convulsive seizures. The labeling of CDP-ethanolamine and phosphoethanolamine diminishes 6 min after the administration of both bicuculline and radioactive ethanolamine whereas that of PE is unaffected in these conditions. Time is very important to this effect; indeed, no differences of the labeling of PE water-soluble precursors can be found in rat cerebellum of normal and convulsing animals 12 min after the administration. The cerebellar pool of CDP-ethanolamine doubles after 6 min of convulsions, which means that unlabeled CDP-ethanolamine forms from a non-radioactive source, such as lipid, may be through the reversal of the ethanolamine phosphotransferase reaction. This effect disappears 12 min after the injection of the convulsant.
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology*; D002531:Cerebellum / Q000378:metabolism*; D003565:Cytidine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism; D005260:Female; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism
D004983:Ethanolamines; D010714:Phosphatidylethanolamines; C006933:CDP ethanolamine; D003565:Cytidine Diphosphate; C005448:phosphorylethanolamine; D001640:Bicuculline