A new model for multiple sclerosis: chronic experimental allergic encephalomyelitis induced by immunization with cerebral endothelial cell membrane.
Multiple sclerosis is considered to be an autoimmune demyelinating disease of the central nervous system. Damage to the blood-brain barrier, of which endothelial cells are the main constituent, occurs in multiple sclerosis, probably due to immunological mechanisms. We report here the results of immune-mediated damage to these cells, produced by immunizing guinea pigs with an endothelial cell membrane fraction. The fraction was obtained from cerebral endothelial cells grown in vitro and was free from myelin basic protein. The immunized animals developed a chronic neurological illness with evidence of delayed hypersensitivity to the cell membrane fraction but not to myelin antigens. Histological examination of the brain in the acute stage showed mononuclear cell infiltrates around blood vessels, while in the chronic phase large areas of demyelination, especially in the periventricular region, were present. This bore a striking similarity to the brain in multiple sclerosis. This may prove to be a useful new animal model for the investigation of the human demyelinating disease.
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000648:ultrastructure; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D002908:Chronic Disease; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000150:complications / Q000209:etiology* / Q000473:pathology; D004727:Endothelium / Q000276:immunology*; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007114:Immunization; D007668:Kidney / Q000473:pathology; D008168:Lung / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008854:Microscopy, Electron; D009103:Multiple Sclerosis; D051381:Rats; D013347:Subcellular Fractions