[Effect of adenine nucleotides on the activator function of streptokinase].
The addition of ATP or 3,5-AMP (but not UTP, GTP, CTP, AMP, 2,3-AMP, ADP, inorganic pyrophosphate) at a final concentration of 10(-1) M into streptokinase solution, pH 7.0 or 9.5, causes a dramatic inhibition of streptokinase-induced fibrinolysis. The specificity of ATP effect is fully lost at pH 3.0, when all nucleotides completely inhibit the activating function of streptokinase. Ribose-5-phosphate causes a similar effect at pH 3.0. The character of nucleotide action on the activating function of streptokinase considerably differs from their influence on proteolytic reactions.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010960:Plasminogen Activators; D013300:Streptokinase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000227:Adenine Nucleotides; D013300:Streptokinase; D010960:Plasminogen Activators
Vliianie adenilovykh nukleotidov na aktivatornuiu funktsiiu streptokinazy.