Determination of desaminosulfamethazine, sulfamethazine, and N4-acetylsulfamethazine by gas chromatography with electron capture detection and confirmation by gas chromatography-chemical ionization mass spectrometry.
An electron capture gas chromatographic method for the determination of sulfamethazine was modified to separate and quantitate simultaneously sulfamethazine and 2 of its metabolites, N4-acetylsulfamethazine and desaminosulfamethazine. The modified method was applied to incurred residues in a veal calf depletion study and to incurred residues in swine tissues. With capillary column gas chromatography-positive ion chemical ionization mass spectrometry, confirmation of the identities of incurred desaminosulfamethazine, N4-acetylsulfamethazine, and sulfamethazine in tissues was obtained from a single injection.
D000818:Animals; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis; D008460:Meat / Q000032:analysis; D013418:Sulfamethazine / Q000031:analogs & derivatives* / Q000032:analysis*; D013552:Swine; D000096926:Benzenesulfonamides
D007202:Indicators and Reagents; D013418:Sulfamethazine; C032633:N-acetylsulfamethazine; C028503:desaminosulfamethazine; D000096926:Benzenesulfonamides