Plastic-adherent progenitor cells in human bone marrow.
Human bone marrow contains plastic-adherent hemopoietic progenitor cells whose plating efficiency is increased by brief (2 h) exposure to methylprednisolone (MP). When subsequently covered with methylcellulose medium, they form colonies of monoblastoid cells. Colony size, but not number, and mature cell production are increased by erythropoietin (epo) and granulocyte-macrophage colony-stimulating factor (GM-CSF). However, colonies do not grow under serum-free conditions. The resistance of plastic-adherent progenitors to treatment with 5-fluorouracil (5FU), their growth pattern, and their capacity to produce granulocytic and erythroid colonies on replating, suggest that they may be similar to the primitive, 5FU-resistant, plastic-adherent progenitor cells (HPP-CFC) in murine marrow.
D001854:Bone Marrow Cells; D002448:Cell Adhesion; D005472:Fluorouracil / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006651:Histocytochemistry; D006801:Humans; D008775:Methylprednisolone / Q000494:pharmacology; D010641:Phenotype; D010969:Plastics
D006133:Growth Substances; D010969:Plastics; D005472:Fluorouracil; D008775:Methylprednisolone