title: Cation effects on taurine release from brain slices: comparison to GABA.
abstract: The effects of cations on the spontaneous and potassium-stimulated taurine release from mouse cerebral cortex slices were assessed with an emphasis on the as yet unestablished calcium dependence of the stimulated release. Spontaneous and stimulated GABA release was analyzed for comparison. A depolarizing concentration (50 mM) of potassium ions caused an approximately 3.5-fold increase in taurine release and the omission of sodium a 6-fold enhancement. GABA release was increased by the same stimuli about 20- and 34-fold, respectively. Omission of calcium ions greatly enhanced basal taurine and GABA release when the medium was supplemented with the calcium chelator ethylenediaminetetraacetate. The potassium stimulation was then abolished, however, with taurine even more readily than with GABA. Magnesium and calcium ions had antagonistic effects on the stimulated release, more clearly with taurine than with GABA. Verapamil abolished the potassium stimulation of both taurine and GABA release, the latter being more sensitive. Although the stimulated taurine release was less in magnitude and had a slower time course than the GABA release, the results are not at variance with the possible neurotransmitter role of taurine.
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism*; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology*; D051379:Mice; D011188:Potassium / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology*; D013654:Taurine / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D013654:Taurine; D005680:gamma-Aminobutyric Acid; D014701:Veratridine; D012964:Sodium; D014700:Verapamil; D008274:Magnesium; D011188:Potassium; D002118:Calcium