Mode of action of bestatin and leupeptin to induce the accumulation of acid soluble peptides in rat liver in vivo and the properties of the accumulated peptides. The important role of bestatin- and leupeptin-sensitive proteases in the protein degradation pathway in vivo.
The chemical properties of acid soluble peptides accumulated in liver or excreted into urine after administration of bestatin or leupeptin to rats were investigated extensively. At the same time, the effects of glucagon on the bestatin-induced accumulation of acid soluble peptides were studied. The results show the important role of bestatin- and leupeptin-sensitive proteases in the degradation pathway of intracellular proteins in vivo.
D000626:Aminopeptidases / Q000037:antagonists & inhibitors; D000818:Animals; D005934:Glucagon / Q000494:pharmacology; D007700:Kinetics; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D009842:Oligopeptides / Q000494:pharmacology*; D010449:Peptide Mapping; D010455:Peptides / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility
D007976:Leupeptins; D009842:Oligopeptides; D010455:Peptides; D011480:Protease Inhibitors; D005934:Glucagon; D000626:Aminopeptidases; D007930:Leucine; C012211:ubenimex; C032854:leupeptin