Effect of cholesterol alpha and beta epoxides on cell killing and transformation.
V-79 Chinese hamster lung fibroblasts and C3H-10T1/2 mouse embryo cells were used to study the toxicity and transformation effects of the 5 alpha,6 alpha, and 5 beta,6 beta-epoxy derivatives of cholesterol. Both epoxides were found to be carcinogenic, and the transformation frequency increased with epoxide concentration and exposure time. The 5 beta,6 beta-epoxide caused a higher level of transformation than the alpha-isomer, and this is consistent with its greater toxicity.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects*; D008168:Lung; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013237:Stereoisomerism
C002415:cholesterol alpha-oxide; D002784:Cholesterol