Absence of detectable calmodulin in cows' milk by a modified gel electrophoresis method.
With a simple, sensitive, and rapid SDS-PAGE procedure, developed during the course of this study, calmodulin was not found in cows' milk. The absence of calmodulin at the level of detection provided by this procedure (.4 micrograms) was unexpected. Because milk is a repository for many protein and enzyme systems, and because the mammary gland is known to contain calmodulin, it was conjectured that at least some "leakage" of the protein into milk would occur, especially into colostrum.
D000818:Animals; D002147:Calmodulin / Q000032:analysis*; D002417:Cattle; D003126:Colostrum / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008892:Milk / Q000032:analysis*; D011247:Pregnancy
D002147:Calmodulin