[Antigenicity study of rokitamycin].
The antigenicity of rokitamycin (TMS-19-Q, TMS) was studied. Rabbits and mice were repeatedly challenged with TMS emulsified with adjuvant to be strongly immunized. Neither IgG nor IgE antibodies were, however, detected in serum from these animals when assayed by PCA and PHA reactions. Furthermore, guinea pigs immunized with TMS together with Freund's complete adjuvant did not exhibit any systemic anaphylactic reactions when elicited with TMS alone. In contrast, specific antibody production against TMS was confirmed in animals immunized with chemically introduced antigen of TMS-carrier protein conjugate, where TMS had antigenic nature as hapten.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D004353:Drug Evaluation, Preclinical; D006168:Guinea Pigs; D006241:Haptens; D006386:Hemagglutination Tests; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007933:Leucomycins / Q000276:immunology*; D008297:Male; D051379:Mice; D015644:Miocamycin / Q000031:analogs & derivatives*; D010323:Passive Cutaneous Anaphylaxis; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000941:Antigens; D006241:Haptens; D007074:Immunoglobulin G; D007933:Leucomycins; D007073:Immunoglobulin E; D015644:Miocamycin; C033383:rokitamycin