title: A defined medium for a fastidious Spiroplasma.
abstract: A defined medium (H-1) was developed for cultivation of the suckling mouse cataract agent, Spiroplasma mirum, a fastidious member of the class Mollicutes that causes cataracts and chronic brain infection in inoculated neonate mice. The H-1 medium was used to show the importance of sphingomyelin as a growth factor for the culture of the spiroplasma in vitro. The growth of Spiroplasma mirum and the pathology it induces in sphingomyelin-rich tissues in vivo may be related to this dependency.
mesh_terms: D000818:Animals; D002386:Cataract / Q000382:microbiology; D003470:Culture Media; D051379:Mice; D013149:Spiroplasma / Q000145:classification / Q000254:growth & development*
chemical_list: D003470:Culture Media