Characterization of the measles virus isolated from the brain of a patient with immunosuppressive measles encephalitis.
Two strains of measles virus with different biologic properties were isolated from the brain of a patient with immunosuppressive measles encephalitis. One strain (Oita-1) grew extremely slowly in Vero cells and did not produce any cell-free virions. The other strain (Oita-2) replicated well, and a small amount of cell-free virus was detected from the culture medium and the freeze-thawed homogenate. Neurovirulence of the Oita-1 strain to mice was, however, stronger than that of the Oita-2 strain. Synthesis of the M protein by the Oita-2 strain was not demonstrable in the infected cells when they were labeled for 24 hr; however, a new protein moiety that was smaller in molecular weight than the M protein and that was not precipitated with monoclonal antibody to M protein was demonstrated by pulse-labeling for 1 hr. Antibodies to the M protein, as well as to other proteins, were detected (by immunoprecipitation) in the serum and cerebrospinal fluid of the patient.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000134:cerebrospinal fluid; D001921:Brain / Q000382:microbiology*; D002675:Child, Preschool; D004660:Encephalitis / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D007108:Immune Tolerance; D008297:Male; D008457:Measles / Q000276:immunology / Q000382:microbiology*; D008459:Measles virus / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis; D015678:Viral Structural Proteins; D014774:Virulence
D000914:Antibodies, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins