Effects of tumor necrosis factor on tumor cell plasma membrane permeability.
Ehrlich ascites tumor cells incubated with rabbit tumor necrosis factor (TNF)-serum showed a temperature-dependent functional impairment of the plasma membrane as reflected by permeability changes. Trypan blue inclusion and Ca2+-transport were considerably increased at hyperthermia temperature. This characteristic of the TNF-plasma membrane interaction may explain the cytotoxicity of TNF.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects*; D006023:Glycoproteins / Q000494:pharmacology*; D013696:Temperature; D014343:Trypan Blue / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D014409:Tumor Necrosis Factor-alpha; D014343:Trypan Blue; D002118:Calcium