Rapid determination of the enantiomers of metoprolol, oxprenolol and propranolol in urine.
A method is described that makes possible the rapid determination of the enantiomers of beta-blocking agents. After extraction from urine samples (at pH 9.9) using toluene, the enantiomers are derivatised with S-(+)-benoxaprofen chloride. The chromatographic separation can be performed on thin-layer plates with toluene-acetone as mobile phase. The derivatives can be detected by measuring the fluorescence (lambda ex = 313 nm,lambda em = 365 nm).
D001711:Biotransformation; D002855:Chromatography, Thin Layer; D006801:Humans; D007202:Indicators and Reagents; D008790:Metoprolol / Q000652:urine*; D010096:Oxprenolol / Q000652:urine*; D011433:Propranolol / Q000652:urine*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D007202:Indicators and Reagents; D010096:Oxprenolol; D011433:Propranolol; D008790:Metoprolol