(1-Pyrene)sulfonyl azide: a fluorescent probe for measuring the transmembrane topology of acetylcholine receptor subunits.
(1-Pyrene)sulfonyl azide (PySA), a fluorescent, lipophilic photolabel, was used as a probe for the transmembrane topology of the acetylcholine receptor (AchR) subunits. Photolabeling of native, alkaline-extracted, and reconstituted AchR membrane preparations resulted in the labeling of all the AchR subunits. However the reconstituted AchR membrane preparations incorporated twice as much PySA into each mole of the AchR complex. Photolabeling of all subunits of the AchR does not appear to alter the agonist concentration response of AchR-mediated cation translocation.
D000818:Animals; D001386:Azides; D002462:Cell Membrane / Q000032:analysis; D005456:Fluorescent Dyes; D010777:Photochemistry; D011950:Receptors, Cholinergic / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014101:Torpedo
D001386:Azides; D005456:Fluorescent Dyes; D011950:Receptors, Cholinergic; C019547:pyrenesulfonyl azide