title: The pH optima for papain and bromelain treatment of red cells.
abstract: The action of papain and bromelain, prepared over a pH range from 4.6 to 8.6, was evaluated for the ability to render red cells agglutinable by five incomplete antibodies of differing blood group specificities using a two-stage technique. The optimal pH for treatment of red cells by activated papain or bromelain was between 5.4 and 5.8. Above this pH range, a fall in serological sensitivity was apparent which was much more pronounced with papain than with bromelain. The optimal pH for enzyme treatment of red cells can be achieved in two-stage techniques, but not in one-stage techniques due to the buffering effect of serum proteins.
mesh_terms: D000371:Agglutination / Q000187:drug effects*; D001788:Blood Grouping and Crossmatching / Q000379:methods / Q000592:standards; D001963:Bromelains / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010206:Papain / Q000494:pharmacology*; D012699:Serology / Q000379:methods / Q000592:standards
chemical_list: D001963:Bromelains; D010206:Papain