title: The beta-delta crossover leading to the beta delta hybrid gene of hemoglobin P-Nilotic is located within 54 base-pairs of the 5' end of exon 2 or between codons 31 and 50.
abstract: The crossover region of the beta delta hybrid gene of the hemoglobin variant Hb P-Nilotic was defined in detail through cloning and sequencing of appropriate DNA segments. The crossover must have occurred without loss of bases within a 54 base-pair stretch of DNA between bases 275 and 330 (or between amino acid residues 31 and 50), indicating that the exon 1 and IVS-1 originate from beta, and exon 2, IVS-2 and exon 3 from delta. The data support the speculation that the IVS-1, in contrast to IVS-2, has no effect on the expression of this hybrid gene.
mesh_terms: D000328:Adult; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D003434:Crossing Over, Genetic; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005914:Globins / Q000235:genetics*; D006455:Hemoglobins, Abnormal / Q000235:genetics*; D006801:Humans
chemical_list: D003062:Codon; D004274:DNA, Recombinant; D006455:Hemoglobins, Abnormal; C049659:hemoglobin P-Nilotic; D005914:Globins