Kinetic studies of four enzyme forms of beta-N-acetylhexosaminidase from embryonic chicken brain.
The separation of four different hexosaminidase forms from embryonic chicken brain (16-day-old) has been performed by ion-exchange chromatography. Two different DEAE-cellulose columns have been used: a first one at pH 7.2 and a second one at pH 6.0. Km and Vmax values were estimated from the Lineweaver-Burk or Dixon plots and ki from the Dixon plots, using N-acetyl-D-glucosamine or N-acetyl-D-galactosamine as inhibitors. In both cases we found a kind of competitive inhibition in which Lineweaver-Burk and Dixon plots curve downwards.
D000118:Acetylglucosaminidase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology*; D002642:Chick Embryo; D006596:Hexosaminidases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D048828:beta-N-Acetyl-Galactosaminidase
D007527:Isoenzymes; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D048828:beta-N-Acetyl-Galactosaminidase