Acyl CoA oxidase is the most suitable marker for hepatic peroxisomal changes caused by treatment of F344 rats with di(2-ethylhexyl)phthalate.
Hepatic peroxisomal changes, caused by treating male Fischer 344 rats with di(2-ethylhexyl)phthalate, were examined by measuring activities of enzymes involved in peroxisomal beta-oxidation, catalase and levels of the 80,000 Da peroxisome proliferation-associated polypeptide. Acyl CoA oxidase activity was increased 2.5-fold after 1 day, and 8-fold after 14 days. Enoyl CoA hydratase activity increased 2-fold after 2 days and 6-fold after 14 days. There were no significant increases in hydroxyacyl CoA dehydrogenase or catalase activities after 3 days of treatment. Thus acyl CoA oxidase activity was the most sensitive marker of early peroxisomal changes. The apparent no-observable-effect level for this change was 0.06 g/kg/day.
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000096:biosynthesis; D042962:Acyl-CoA Oxidase; D000818:Animals; D002374:Catalase / Q000096:biosynthesis; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004746:Enoyl-CoA Hydratase / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008830:Microbodies / Q000187:drug effects* / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D010088:Oxidoreductases / Q000096:biosynthesis*; D010795:Phthalic Acids / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
D010795:Phthalic Acids; D004051:Diethylhexyl Phthalate; D010088:Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; D002374:Catalase; D042962:Acyl-CoA Oxidase; D004746:Enoyl-CoA Hydratase