Monoclonal antibody immunoaffinity chromatography of the nerve growth factor from snake venoms.
1. Pure monoclonal antibodies to Vipera lebetina venom nerve growth factor have been isolated by affinity chromatography using CNBr-agarose bound antigen. 2. Nerve growth factors from ten snake venoms (Vipera lebetina, Vipera russellii, Vipera berus berus, Vipera ursini, Echis carinatus, Agkistrodon halys, Bungarus caeruleus, Naja naja oxiana, Naja naja, Naja naja atra) were purified using monoclonal antibodies against NGF linked to BrCN-activated agarose.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D002642:Chick Embryo; D002846:Chromatography, Affinity; D003435:Crotalid Venoms / Q000032:analysis; D004546:Elapid Venoms / Q000032:analysis; D009414:Nerve Growth Factors / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D012910:Snake Venoms / Q000032:analysis*; D014757:Viper Venoms / Q000032:analysis
D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D004546:Elapid Venoms; D009414:Nerve Growth Factors; D012910:Snake Venoms; D014757:Viper Venoms