Influence of metal chelators on metalloenzymes.
The influence of chelating agents (1 mmol/kg/day X 6,i.p.) on trace metal mobilization and activities of certain metalloenzymes was investigated in rats. Calcium disodium ethylenediamine tetraacetate (CaNa2EDTA) and calcium trisodium diethylenetriamine pentaacetate (CaNa3DTPA) enhanced urinary excretion of Zn, while sodium 2,3-dimercaptopropane-1-sulfonate (NaDMPS) and sodium diethyldithiocarbamate (NaDDC) increased that of Cu. The activity of Zn-metalloenzymes-blood delta-aminolevulinic acid dehydratase (delta-ALA-D), plasma alkaline phosphatase (ALP) and that of Cu-metalloenzyme-plasma amine oxidase was decreased as a consequence of chelation therapy. However, hepatic levels of delta-ALA-D, ALP and alcohol dehydrogenase remained unaffected by chelation. The activity of hepatic Fe-metalloenzyme-catalase was increased by polyaminocarboxylic acids and lowered by thiol chelators. The metal chelators decreased the hepatic glutathione levels.
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D002374:Catalase / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008670:Metals / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D000623:Porphobilinogen Synthase / Q000378:metabolism; D051381:Rats; D015032:Zinc / Q000378:metabolism
D002614:Chelating Agents; D008670:Metals; D003300:Copper; D007501:Iron; D000426:Alcohol Dehydrogenase; D002374:Catalase; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors; D000469:Alkaline Phosphatase; D000623:Porphobilinogen Synthase; D005978:Glutathione; D015032:Zinc