The effect of endothelial cell proliferation in vitro.
The proliferation of bovine aortic endothelial cells is highly stimulated by using a plasma clot instead of glass or plastic as a substrate. The cells form a dense monolayer which is usually under grown by a second layer of endothelial cells showing a more reticular growth pattern. When endothelial cells attached to a plasma clot are covered with a second layer of coagulated plasma, the cells arrange to shapes which bear a great resemblance to capillary structures. It seems that this endothelial cell property is only expressed when the entire cell surface is forced to interact with a surrounding adhesive matrix.
D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D004727:Endothelium / Q000166:cytology*; D010949:Plasma