Effects of prostaglandin biosynthesis inhibitors and ouabain on duodenal mucosa and HCO3- secretion in rats.
A single injection (s.c.) of prostaglandin biosynthesis inhibitors such as indomethacin (5 mg/kg), aspirin (200 mg/kg) and quinacrine (100 mg/kg) or a Na+.K+ ATPase inhibitor such as ouabain (10 mg/kg) significantly reduced the adaptive increase of HCO3- output caused by acid in the duodenum of anesthetized rats. These agents had no effect on basal duodenal HCO3- secretion and histamine-stimulated gastric acid secretion. Either of these agents, when given alone, had no effect on the duodenal mucosa of conscious rats, but produced damage in the proximal duodenum within 8 hr when given together with histamine (40 mg/kg X 3, s.c., every 2.5 hr). A significant relationship was found between the degrees of inhibition of acid-induced HCO3- output and the severity of duodenal lesions induced by these drugs (r = 0.8620, P less than 0.01). These results suggest that an impairment of the mechanisms related to acid-induced HCO3- secretion may be particularly relevant to the pathogenesis of duodenal lesions.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001639:Bicarbonates / Q000378:metabolism*; D004347:Drug Interactions; D004386:Duodenum / Q000187:drug effects; D005753:Gastric Mucosa / Q000187:drug effects*; D006632:Histamine / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D010042:Ouabain / Q000494:pharmacology*; D011448:Prostaglandin Antagonists / Q000494:pharmacology*; D011796:Quinacrine / Q000494:pharmacology; D051381:Rats
D001639:Bicarbonates; D011448:Prostaglandin Antagonists; D010042:Ouabain; D006632:Histamine; D011796:Quinacrine; D001241:Aspirin; D007213:Indomethacin