Kinetics of suicide substrate:enzyme inactivation. Methylenecyclopropaneacetyl-CoA and general acyl-CoA dehydrogenase.
Kinetics of inactivation of general acyl-CoA dehydrogenase from pig kidney by methylenecyclopropaneacetyl-CoA have been analyzed using the theoretical treatment and exact steady-state kinetic solutions reported by Tatsunami (Tatsunami, S., Yago, N., and Hosoe, M. (1981) Biochim. Biophys. Acta 662, 226-235). Thus practical application of these analytical solutions for an important class of enzyme:substrate reactions has been demonstrated for the first time.
D000105:Acetyl Coenzyme A / Q000031:analogs & derivatives* / Q000494:pharmacology; D044944:Acyl-CoA Dehydrogenases / Q000037:antagonists & inhibitors*; D000818:Animals; D001665:Binding Sites; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008962:Models, Theoretical; D011485:Protein Binding; D013552:Swine
C010750:methylenecyclopropyl acetyl-CoA; D000105:Acetyl Coenzyme A; D044944:Acyl-CoA Dehydrogenases