Serial sectioning techniques for a modified LKB Historesin.
A glycol methacrylate-based plastic that is capable of producing serial sections has been introduced by LKB. This plastic, provided in the LKB 2218-500 Historesin Embedding Kit, has been tested in our laboratory for its ribbon forming capacity. Various block sizes, concentrations of the softening agent polyethylene glycol 400 (PEG), and tissue types have been examined to determine the optimal conditions for ribbon formation. Although unmodified LKB Historesin is capable of forming ribbons, these ribbons often break. The addition of PEG to the embedding solution enhances ribbon formation. When sectioning with glass knives the best results are achieved with the addition of 0.2 ml of PEG/5.0 ml of embedding medium. A conventional AO rotary microtome can be used to produce ribbons if, in addition to the added PEG (optimal concentration 0.25-0.30 per 5 ml of embedding medium) a thin layer of dental wax is added to the upper and lower surfaces of the block. Ribbons form more easily on microtomes, such as the LKB Historange, that have a retractable specimen arm. If serial sections are to be produced it is very important that the upper and lower faces of blocks be parallel.
D000179:Acrylates; D008689:Methacrylates; D008867:Microtomy / Q000379:methods*; D011092:Polyethylene Glycols; D011933:Reagent Kits, Diagnostic
D000179:Acrylates; D008689:Methacrylates; D011933:Reagent Kits, Diagnostic; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate