A comparison of an enzyme-linked immunosorbent assay and counter current electrophoresis for the detection of bovine serum albumin in virus vaccines.
A monoclonal antibody directed against bovine serum albumin (BSA) has been developed and used in an enzyme-linked immunosorbent assay (ELISA) system for the detection of BSA in virus vaccines. The results correlated well with those obtained with a counter current electrophoresis system which has been employed routinely for this purpose. The ELISA was slightly more sensitive and more readily applicable to the screening of large numbers of samples but could not be used in the presence of certain stabilizers.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D003378:Counterimmunoelectrophoresis; D004340:Drug Contamination / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D007122:Immunoelectrophoresis; D012710:Serum Albumin, Bovine / Q000032:analysis*; D014765:Viral Vaccines / Q000032:analysis / Q000592:standards*
D000911:Antibodies, Monoclonal; D014765:Viral Vaccines; D012710:Serum Albumin, Bovine