Mucus stabilization in the urinary bladder.
Mucus in the urinary bladder may act as an important intrinsic defence mechanism by shielding the epithelial layer from pathogens. Visualization of any such layer of mucus using electron microscopy has not been possible, as preparative procedures in current use result in the loss or distortion of mucus. We evaluated methods reported to stabilize polysaccharide layers and demonstrated that pretreatment of rat bladder tissue with anti-mucus antiserum, minimized the collapse of the mucus that normally occurred during dehydration of the tissue for electron microscopic examination. Under these conditions, mucus was stabilized as a layer closely adherent to the epithelium.
D000818:Animals; D000906:Antibodies; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D005615:Freezing; D008855:Microscopy, Electron, Scanning; D009093:Mucus / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D001743:Urinary Bladder / Q000276:immunology / Q000648:ultrastructure*
D000906:Antibodies