title: Isolation and characterization of a new zinc-binding protein from albacore tuna plasma.
abstract: The protein responsible for sequestering high levels of zinc in the plasma of the albacore tuna (Thunnus alalunga) has been isolated by sequential chromatography. The glycoprotein has a molecular weight of 66,000. Approximately 8.2% of its amino acid residues are histidines. Equilibrium dialysis experiments show it to bind 3 mol of zinc/mol of protein. The stoichiometric constant for the association of zinc with a binding site containing three histidines was determined to be 10(9.4). This protein is different from albumin and represents a previously uncharacterized zinc transport protein.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D011485:Protein Binding; D014413:Tuna; D015032:Zinc / Q000097:blood*
chemical_list: D000596:Amino Acids; D002352:Carrier Proteins; C017800:zinc-binding protein; D015032:Zinc