Analysis of the lipoteichoic-acid-like macroamphiphile from Bifidobacterium bifidum subspecies pennsylvanicum by one- and two-dimensional 1H- and 13C-NMR spectroscopy.
The native lipoteichoic-acid-like amphiphile from Bifidobacterium bifidum subspecies pennsylvanicum and its basic glycan moiety, obtained by alkaline hydrolysis, were studied by 1H-, 13C-, and 31P-NMR, DEPT (distorsionless enhancement by polarization transfer), 1H-1H correlation spectroscopy and 13C-1H shift correlation NMR spectroscopy. The results are consistent with the structure elucidated by chemical analysis (Fischer, W., preceding paper in this journal): (formula; see text) and establish the structures of the repeating units independently. In addition to characteristic shifts in the 13C-NMR spectrum, the sites of phosphodiester and alanine ester bonds are also manifest in the 1H-NMR spectrum. Substitution of the glycerophosphate residues can be recognized in the 31P-NMR spectrum also.
D001644:Bifidobacterium / Q000032:analysis*; D002247:Carbon Isotopes; D006859:Hydrogen; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010712:Phosphatidic Acids / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis; D012492:Salts; D013682:Teichoic Acids / Q000032:analysis*
D002247:Carbon Isotopes; D008070:Lipopolysaccharides; D010712:Phosphatidic Acids; D011134:Polysaccharides; D012492:Salts; D013682:Teichoic Acids; C009900:lipoteichoic acid; D006859:Hydrogen