title: Molecular cloning and cDNA structure of the regulatory subunit of type I cAMP-dependent protein kinase from rat brain.
abstract: Complementary DNA (cDNA) clones encoding the regulatory subunit of the type I cAMP-dependent protein kinase (R-I) were isolated by screening of rat brain cDNA libraries. A 1.5-kilobase (kb) cDNA insert containing the entire coding region was sequenced and full amino acid sequence has been deduced from the nucleotide sequence. The clone encodes for a protein of 380 amino acids that shows 97% homology to the bovine R-I subunit. Northern blot analysis demonstrated two major mRNA species (2.8 and 4.4 kb in size) in rat brain and liver.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D011494:Protein Kinases / Q000235:genetics*; D051381:Rats
chemical_list: D046911:Macromolecular Substances; D004247:DNA; D011494:Protein Kinases