Plasma amino-acids analysis: effects of delayed samples preparation and of storage.
This paper describes changes occurring in plasma amino-acid concentrations of: samples which are not prepared for analysis immediately after blood collection and samples stored for 5 to 6 months in the form of either plasma, or of deproteinized plasma, or of deproteinized and at pH 2.2 buffered plasma. Results showed that in order to avoid these changes, plasma should be deproteinized and buffered as soon as possible after blood collection. When analysis could not be performed immediately, storage of samples in a freezer at -18 degrees C in the form of deproteinized and at pH 2.2 buffered plasma showed after 5 to 6 months the best recovery of the initial concentrations, but did not exclude all changes.
D000596:Amino Acids / Q000097:blood*; D001793:Blood Preservation / Q000379:methods; D001798:Blood Proteins; D001800:Blood Specimen Collection; D002021:Buffers; D005615:Freezing; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010949:Plasma; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature
D000596:Amino Acids; D001798:Blood Proteins; D002021:Buffers