Studies on ornithine decarboxylase from liver, kidney and tumoral tissues during activity decay following cycloheximide administration.
We have studied the activity, thiol-dependency and Km of ornithine decarboxylase (ODC) from the following sources: liver of rats subjected to partial hepatectomy or administered thioacetamide, the rat 3924A Morris hepatoma, the rat AH130 Yoshida ascites hepatoma, a mouse transplantable mammary carcinoma and kidney of rats administered testosterone propionate. In order to detect possible changes occurring during in vivo ageing of this enzyme we inhibited protein synthesis with cycloheximide. A gradual decrease of Km during ageing was observed in ODC from liver.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D005260:Female; D006498:Hepatectomy; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D008115:Liver Regeneration; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013853:Thioacetamide / Q000494:pharmacology
D013853:Thioacetamide; D003513:Cycloheximide; D009955:Ornithine Decarboxylase