Characterization of acid and neutral alpha-mannosidases in bull semen and reproductive organs.
Acid and neutral alpha-mannosidase activities were studied in the bull reproductive tissues, isolated spermatozoa, epididymal and seminal vesicle secretion and seminal plasma. The acid enzyme in the seminal plasma mainly derived from the epididymal secretion, while the neutral one was enriched in the sperm cells. The latter activity in the seminal plasma appears to be due to an enzyme released from the cytoplasmic droplets in the epididymis. The acid enzyme had a molecular weight of 220,000-320,000, pI 7.3-6.0 and an optimum at pH 4.0. It was sensitive to swainsonine but was stimulated by Zn2+. The neutral enzyme had a molecular weight of 360,000-460,000, pI 5.4-4.7 and showed double optima at pH 5.5 and 6.0-7.0. It was resistant to swainsonine but was markedly activated by Co2+ or Fe2+. The neutral enzyme was also more sensitive to thermal inactivation than the acid one.
D000818:Animals; D002417:Cattle; D004822:Epididymis / Q000201:enzymology* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008361:Mannosidases / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D009928:Organ Specificity; D012661:Semen / Q000201:enzymology*; D012669:Seminal Vesicles / Q000201:enzymology* / Q000378:metabolism; D013094:Spermatozoa / Q000201:enzymology*; D043323:alpha-Mannosidase
D007527:Isoenzymes; D008361:Mannosidases; D043323:alpha-Mannosidase