The product of the Drosophila zeste gene binds to specific DNA sequences in white and Ubx.
Three different segments of the zeste coding sequence were inserted in an expression vector and antibodies were raised against the resulting zeste-beta galactosidase hybrid proteins. The antibodies were used to analyse the zeste protein produced in bacteria from a different expression vector containing the entire zeste coding region. The major products made in bacteria as well as the products of in vitro translation of zeste RNA migrate anomalously upon SDS--acrylamide gel electrophoresis. Specific DNA fragments from the white and Ubx gene co-immunoprecipitate with zeste protein. At least two independent zeste binding sites are found in a 250-bp interval of the white regulatory region that contains also the sites of wsp mutations, which are known to be deficient in zeste interaction.
D000818:Animals; D000906:Antibodies; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D005796:Genes; D005809:Genes, Regulator; D005822:Genetic Vectors; D007301:Insect Hormones / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D013045:Species Specificity
D000906:Antibodies; D004268:DNA-Binding Proteins; D007301:Insect Hormones; D004247:DNA