title: Enhancement of human monocyte and peritoneal macrophage chemiluminescence activities in women with endometriosis.
abstract: Preliminary reports indicate that products of human mononuclear phagocytes may contribute to the infertility associated with endometriosis. To determine whether the generation of reactive oxygen metabolites by blood monocytes and peritoneal macrophages is altered in women with endometriosis, the present study evaluated luminol-enhanced chemiluminescence (CL) in cells at rest and following stimulation with phorbol myristate acetate (PMA) or serum-opsonized zymosan (SOZ). Peripheral venous blood and peritoneal fluid samples were collected from 60 infertile women undergoing diagnostic laparoscopy at midluteal phase and mononuclear phagocytic cell fractions were obtained by density gradient centrifugation. Whereas there was no significant difference between resting CL values in peripheral blood monocytes collected from women with and without endometriosis, PMA- and SOZ-stimulated monocyte CL was significantly greater in endometriosis patients. In contrast, there was a significant elevation in resting CL values when peritoneal macrophages from endometriosis patients were compared with macrophages obtained from patients with normal pelvic organs. It appears that chronic stimulation of macrophages in the peritoneal cavity provokes constitutive release of large quantities of reactive oxygen products in women with endometriosis. This may occur secondary to the accumulation of activated monocytes into the peritoneal cavity.
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000166:cytology / Q000378:metabolism; D004715:Endometriosis / Q000150:complications / Q000378:metabolism*; D005260:Female; D005609:Free Radicals; D006801:Humans; D007247:Infertility, Female / Q000209:etiology; D008163:Luminescent Measurements; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism
chemical_list: D005609:Free Radicals; D010100:Oxygen