Assay of crystal violet binding for rapid identification of virulent plasmid-bearing clones of Yersinia enterocolitica.
A rapid, reliable, and simple method based on the binding of crystal violet (CV) is described for differentiating virulence-plasmid-bearing strains of Yersinia enterocolitica from their plasmidless derivatives. As with other plasmid-mediated properties of this organism, the binding of CV occurs at 37 degrees C but not at 25 degrees C. The CV-binding technique provides a simple and efficient means of screening Y. enterocolitica for virulence and for identifying individual plasmid-bearing colonies.
D000818:Animals; D005840:Gentian Violet / Q000378:metabolism*; D008297:Male; D051379:Mice; D010957:Plasmids; D014774:Virulence; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*
D005840:Gentian Violet