Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene.
We isolated and characterized the 5' region of the mouse c-Ki-ras gene, including a 5' untranslated exon (exon 0). These studies used genetic material from Y1 mouse adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at the 5' ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a genomic fragment within the 5' region which exhibits bidirectional promoter activity.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D005091:Exons; D005784:Gene Amplification; D051379:Mice; D011401:Promoter Regions, Genetic; D011519:Proto-Oncogenes; D014158:Transcription, Genetic
D004247:DNA