Effects of sperm-reactive monoclonal antibodies on the cervical mucus penetrating ability of human spermatozoa.
A group of monoclonal antibodies (Mabs) was tested by immunobead binding and indirect immunofluorescence to determine its reactivity with antigenic moieties present either on the surface of motile human spermatozoa or internally. Those Mabs reactive with the surface of motile spermatozoa impaired their ability to penetrate cervical mucus in vitro, while Mabs solely reactive with freeze-thawed, nonmotile sperm (presumptive subsurface epitopes) were without effect on sperm function.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002417:Cattle; D002584:Cervix Uteri / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D009092:Mucous Membrane / Q000276:immunology; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex