Replication of DNA containing apurinic sites in human and mouse cells probed with parvoviruses MVM and H-1.
We studied the effect of apurinic sites on DNA replication in mouse and human cells, using parvoviruses MVM (minute virus of mice) and H-1 as probes. Although apurinic sites are efficient blocks to the replication of these single-stranded DNA viruses in vivo, depurinated parvoviruses can be reactivated if host cells have been preexposed to a subtoxic dose of UV light. The target of this conditional reactivation process is the conversion of depurinated input DNA into double-stranded replicative forms; the concomitant increase in viral mutagenesis strongly suggests that apurinic sites can be bypassed in mammalian cells.
D000818:Animals; D004261:DNA Replication / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D051379:Mice; D010321:Parvoviridae / Q000235:genetics / Q000528:radiation effects; D011685:Purine Nucleotides / Q000235:genetics*; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D011685:Purine Nucleotides