Kinetics of binding of lucensomycin to natural and artificial membranes.
The binding of the polyenic antibiotic lucensomycin to native or modified human erythrocyte ghosts and to model membranes has been studied by monitoring the absorbance variations of the polyene at 320 nm. The non-steroidal components of the membranes (such as proteins and phospholipids) seem to affect the rate of the individual reaction steps leading to the formation of cholesterol-lucensomycin complexes rather than the ratio among these heterologous aggregates at equilibrium.
D000935:Antifungal Agents / Q000378:metabolism*; D001665:Binding Sites; D002784:Cholesterol; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008155:Lucensomycin / Q000378:metabolism*; D008433:Mathematics; D008563:Membrane Lipids / Q000378:metabolism; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D013053:Spectrophotometry
D000935:Antifungal Agents; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D008155:Lucensomycin; D002784:Cholesterol