Purification and characterization of a novel human angiogenic factor (h-AF).
Serum-free supernatants of the human melanoma cell line A-375/2 contain an angiogenic activity as detected by the chorioallantois membrane assay which does not induce proliferation of cultured endothelial cells. This human angiogenic factor (h-AF) was purified by immunoaffinity chromatography as well as by conventional chromatographic procedures. A monoclonal antibody designated 5F4 was raised against h-AF, which binds but does not neutralize angiogenic activity. h-AF consists of a protein at MW 67 kD which focuses at pH 5.0. By biological and biochemical criteria it is shown that h-AF differs from other known growth factors.
D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification*; D002460:Cell Line; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000302:isolation & purification*; D006801:Humans; D008545:Melanoma; D008970:Molecular Weight
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances