Poly(I).poly(C), a potential drug carrier for the antitumor agent mitoxantrone: in vitro drug binding study.
Coupling of mitoxantrone, a new antitumor agent, to a macromolecular carrier system may improve the drug's selectivity of action and pharmacokinetic properties. We have studied in vitro binding of mitoxantrone to poly(I).poly(C), a macromolecular, double-stranded homoribopolymer, by equilibrium dialysis and high-performance liquid chromatography (HPLC). Results showed high binding affinity for mitoxantrone to poly(I).poly(C) (Kd = 1.05 X 10(-6) M), the calculated number of mitoxantrone-binding sites is 60 per molecule poly(I).poly(C). In view of the good tolerance in clinical studies, poly(I).poly(C) may thus be a useful drug carrier for mitoxantrone. A mitoxantrone:poly(I).poly(C) ratio of 1:30 (w/w) is recommended for therapeutic studies.
D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008942:Mitoxantrone / Q000032:analysis / Q000378:metabolism*; D011070:Poly I-C / Q000032:analysis / Q000378:metabolism*; D011107:Polylysine / Q000032:analysis / Q000378:metabolism*
D011107:Polylysine; C040125:poly ICL; D008942:Mitoxantrone; D011070:Poly I-C