Effect of aspirin and vitamin E on phase separation in calf lens homogenate.
Vitamin E (d-alpha-tocopherol) and aspirin were evaluated for their effects on the phase separation temperature, Tc, of calf lens cytoplasm. Vitamin E and aspirin were added to nuclear homogenate and the change in Tc was measured. Aspirin had no measurable effect on Tc. Vitamin E lowered Tc at a rate of -22 degrees C M-1 l-1. The results suggest that the effects of aspirin on cataract are unrelated to phase separation and that vitamin E may act on the phase separation in precataractous lens cells.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001831:Body Temperature / Q000187:drug effects; D002386:Cataract / Q000517:prevention & control; D002417:Cattle; D007834:Lasers; D007908:Lens, Crystalline / Q000187:drug effects*; D009885:Ophthalmology / Q000295:instrumentation; D014810:Vitamin E / Q000494:pharmacology*
D014810:Vitamin E; D001241:Aspirin