Evidence of a direct action of triiodothyronine (T3) on the cell membrane of GH3 cells: an electrophysiological approach.
Electrophysiological experiments demonstrate that triiodothyronine (T3) exerts a direct effect on the membrane of a strain of cultured rat pituitary tumor cells, GH3/B6. These cells respond to pressure application of T3 (2-5 nl, concentration 1 X 10(-10) M) with an increase in the membrane resistance (Rm) and a hyperpolarization. Spontaneously firing cells become silent.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D004553:Electric Conductivity; D004594:Electrophysiology; D008564:Membrane Potentials / Q000187:drug effects; D010911:Pituitary Neoplasms / Q000503:physiopathology*; D051381:Rats; D014284:Triiodothyronine / Q000494:pharmacology*
D014284:Triiodothyronine