[Conditions of the reversibility of metaphase arrest and induction of multi-polar mitoses after treatment with nocodazole].
Nocodazole at a concentration 0.02 mcg/ml or higher arrests PE cells (pig kidney embryo cells) in K-metaphase. Accumulation of mitotic cells by incubation with 0.02 or 0.6 mcg/ml nocodazole occurs linearly and at the same rate during 12-16 hours. After nocodazole is removed, the mitotic index is resumed to the normal rate. The maximum time of the reversible mitotic arrest in PE cells in 16 hours. After the incubation of cells with 0.2 mcg/ml nocodazole, the time of the reversible mitotic arrest is 12 hours. After the incubation of cells with 0.02 or 0.2 mcg/ml nocodazole, no multipolar mitoses are observed. After the 4 hours incubation with 0.6 mcg/ml nocodazole, multipolar mitotic figures are observed 1.5-2.5 hours after drug removal. It is concluded that the induction of multipolar divisions requires no prolonged mitotic arrest, but it may be caused by a complete depolymerization of spindle microtubules.
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002478:Cells, Cultured; D007668:Kidney; D008677:Metaphase / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects*; D008940:Mitotic Index / Q000187:drug effects; D015739:Nocodazole; D013552:Swine
D001562:Benzimidazoles; D015739:Nocodazole
Izuchenie usloviĭ obratimosti metafaznogo bloka i induktsii mnogopoliusnykh mitozov pri deĭstvii nokodazola.