[Stabilization of native and immobilized urease].
The stability of native and immobilized urease isolated from Staphylococcus saprophyticus was studied at 4 degrees and 25 degrees C. The activity yield was 20% and 1.4% on the enzyme immobilization in albumin gel and latex membrane, respectively. Inactivation of native microbial urease proceeded 10 times slower in the solution containing 1 mM EDTA and 30 mM sodium sulfite. This solution contributed to a great extent to stabilization of immobilized urease both during storage in the phosphate buffer solution and in case of lyophilization.
D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D013210:Staphylococcus / Q000201:enzymology*; D014510:Urease / Q000037:antagonists & inhibitors / Q000302:isolation & purification*
D004800:Enzymes, Immobilized; D014510:Urease
Stabilizatsiia nativnoĭ i immobilizovannoĭ ureazy.