Stress induced biosynthesis of a 31 kd-glycoprotein in goldfish brain.
The de novo protein synthesis in goldfish brain has been studied under defined stress conditions and after training in an active swimming task. One- and two-dimensional gel electrophoresis of the brain proteins previously labelled in vivo with 35S-methionine indicated that the synthesis of one distinct protein increases after stress but not after successful training. This protein is a glycoprotein with an apparent molecular weight of 31 kd and an isoelectric point of pH 5. It is discussed that the protein may be the ACTH precursor "pro-opiomelanocortin".
D000818:Animals; D001362:Avoidance Learning; D001921:Brain / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006054:Goldfish; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D013315:Stress, Psychological / Q000378:metabolism*
D006023:Glycoproteins; D009419:Nerve Tissue Proteins