title: Ornithine decarboxylase activity in foetal rat brain cells and in the mouse embryo fibroblasts C3H/10T1/2 CL8 cells: differences in response to medium change and to the tumor promoter TPA.
abstract: 12-O-tetradecanoyl-phorbol-13-acetate (TPA) induced ornithine decarboxylase (ODC, EC 4.1.1.17) in normal, preneoplastic and malignant rat brain cells in culture, but treatment with phorbol, acetate or medium shift resulted in a similar response. Medium shift induced ODC activity in C3H/10T1/2 CL8 cells 4 and 12 hr after treatment. TPA induced only the 12 hr peak. ODC induction in C3H/10T1/2 CL8 cells was completely inhibited by cycloheximide and actinomycin D. Addition of alpha-amanitin abolished the 12 hr peak, but the TPA induced ODC activity was only partly inhibited. ODC induction by TPA was lower in C3H/10T1/2 CL8 cells initiated with 3-methyl-cholanthrene (MCA). ODC increased with TPA up to 10(-7) M and decreased at higher concentrations of TPA.
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology* / Q000201:enzymology; D001932:Brain Neoplasms / Q000139:chemically induced / Q000201:enzymology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D003470:Culture Media; D004622:Embryo, Mammalian; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000201:enzymology*; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D003470:Culture Media; D008748:Methylcholanthrene; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate