The utilization of biotin-antibiotin interaction for the detection of antibody to hepatitis B surface antigen (anti-HBs).
A biotin-antibiotin solid-phase enzyme-linked immunoassay for the detection and quantitation of the antibody to hepatitis B surface antigen (anti-HBs) is described. The assay utilizes hepatitis B surface antigen as a solid-phase 'capture' reagent and a mixture of biotinylated HBsAg and antibiotin-conjugated horseradish peroxidase as a detector reagent. The assay was compared to a commercial enzyme immunoassay (AUSAB EIA) which used the biotin-avidin system for anti-HBs detection. The two assays were found to measure the same molecules and to correlate well regarding anti-HBs titers.
D001710:Biotin / Q000276:immunology; D001782:Blood Donors; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D001710:Biotin