title: A novel 60-kDa smooth muscle protein that binds filamin-actin filament complex.
abstract: From the low salt-extracted debris of bovine stomach smooth muscle, a protein having a molecular mass of 60 kDa in SDS-PAGE was newly isolated. Co-sedimentation assay with actin filaments and several actin binding proteins such as filamin, alpha-actinin, caldesmon and fodrin showed that this protein co-sediments with actin only in the presence of filamin. Falling ball viscometric assay showed that this protein increases the viscosity of actin-filamin solution in a dose-dependent manner. Immunoblotting analysis showed specific localization of this protein in smooth and striated muscles.
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002498:Centrifugation; D002845:Chromatography; D003285:Contractile Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D064448:Filamins; D007118:Immunoassay; D008840:Microfilament Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009130:Muscle, Smooth / Q000032:analysis*; D013270:Stomach; D014783:Viscosity
chemical_list: D000199:Actins; D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D009124:Muscle Proteins