Application of the fluorometric DNA quantitative assay using 4'6-diamidine-2-phenylindole for the evaluation of lymphocyte blastogenic response.
A fluorometric DNA microassay using the fluorescent reagent 4'6-diamidine-2-phenylindole dihydrochloride (DAPI) was developed for evaluating a lymphocyte DNA synthesis assay. Triton X-100 (0.05 per cent) was added to the cultured lymphocytes, then DAPI (0.8 micrograms ml-1) was added as a DNA-specific fluorescent intercalating agent. Results are expressed as fluorescence intensity (FI) and stimulation index. FIs measured by DAPI fluorometric assay were highly correlated with [3H]-thymidine incorporation (r = 0.868, P less than 0.01, n = 28). The method is simple, reliable and widely applicable for evaluating blastogenic responses.
D000818:Animals; D002417:Cattle / Q000235:genetics / Q000276:immunology*; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes; D005470:Fluorometry; D007211:Indoles; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA