Colistin (polymyxin E)--induced cell leakage in Mycobacterium aurum.
Addition of 100 micrograms/ml of colistin (polymyxin E) to an actively-growing culture of Mycobacterium aurum caused apparent loss of viability starting after 20 min of drug addition, whereas significant Mg++ efflux was apparent within 5 min of drug addition. Therefore, Mg++ efflux preceded cell death by at least 15 min. These data confirmed earlier reports showing that colistin interacted with the cytoplasmic membrane of mycobacteria.
D002462:Cell Membrane / Q000187:drug effects; D003091:Colistin / Q000494:pharmacology*; D008274:Magnesium / Q000378:metabolism; D009161:Mycobacterium / Q000187:drug effects* / Q000648:ultrastructure
D008274:Magnesium; D003091:Colistin