Isolation and properties of androgenic gland hormone from the terrestrial isopod, Armadillidium vulgare.
The androgenic gland hormone (AGH) was purified from the male reproductive organs, including the androgenic glands, of Armadillidium vulgare by pH adjustment, ammonium sulfate precipitation, gel filtration, ion-exchange chromatography, and three kinds of high-performance liquid chromatography (HPLC). AGH consisted of two molecular forms, AGH I and AGH II. They were judged as homogeneous by HPLC, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and isoelectrofocusing, though their N-terminal residues were not detected. They seem to be monomeric proteins consisting of 157 and 166 amino acid residues, having molecular weights of 17,000 +/- 800 and 18,300 +/- 1000, and having isoelectric points of about 4.5 and 4.3, respectively. The activities of AGH I and AGH II were almost the same and their respective specific activities were 560 and 430 times that of the crude extract.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003445:Crustacea / Q000502:physiology*; D004702:Endocrine Glands / Q000502:physiology*; D005837:Genitalia, Male / Q000502:physiology*; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones / Q000302:isolation & purification*; D008297:Male; D008970:Molecular Weight; D004490:Xenarthra / Q000502:physiology*
D000596:Amino Acids; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones; C053644:androgenic gland hormone (Armadillidium)