title: Choline incorporation by Schistosoma mansoni: distribution of choline metabolites during development and after sexual differentiation.
abstract: Choline metabolism was investigated in Schistosoma mansoni during the main phases of its development, namely, schistosomula, 11- and 15-day-old worms, and adults. At the physiological choline concentration used in the assay (20 microM), betaine was, along with phosphatidylcholine, one of the most abundant choline metabolites, revealing considerable choline oxidation activity. Very little radioactivity was associated with CDP-choline, whereas a sustained incorporation into phosphocholine occurred. These results provide good evidence that CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) plays a regulatory role in the de novo pathway of phosphatidylcholine biosynthesis. During development, the incorporation of choline into its various metabolites was maximal in 11-day-old worms. At this stage, the oxidative pathway predominated over the Kennedy pathway, whereas at all other stages the de novo phosphatidylcholine biosynthesis was predominant. Furthermore, choline incorporation into betaine was much more important in the adult female worm than in the male, indicating a major difference in choline incorporation and distribution between the 2 sexes of the adult worms.
mesh_terms: D000818:Animals; D001622:Betaine / Q000378:metabolism; D002250:Carbon Radioisotopes; D002794:Choline / Q000378:metabolism*; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D005260:Female; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis; D010767:Phosphorylcholine / Q000032:analysis; D012550:Schistosoma mansoni / Q000378:metabolism*; D012733:Sex Differentiation
chemical_list: D002250:Carbon Radioisotopes; D010713:Phosphatidylcholines; D010767:Phosphorylcholine; D001622:Betaine; D003566:Cytidine Diphosphate Choline; D002794:Choline