[Selection of mutants of Clostridium acetobutylicum defective in production of acetone].
The butyrate analogue 2-bromobutyrate is toxic toward Clostridium acetobutylicum. After mutation using nitrosoguanidine, mutants resistant to the suicide co-substrate 2-bromobutyrate were selected. Such mutants no longer produced acetone, whereas the synthesis of butanol and ethanol was unaffected. Enzymatic analysis of a 2-bromobutyrate-resistant mutant showed a low level of coenzyme A transferase and of acetoacetate decarboxylase, the two enzymes implicated in the biosynthesis of acetone, whereas the level of hydrogenase was unaffected.
D000096:Acetone / Q000096:biosynthesis*; D001965:Bromides / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D003013:Clostridium / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004352:Drug Resistance, Microbial; D009154:Mutation
D001965:Bromides; D002087:Butyrates; D000096:Acetone
Sélection de mutants de Clostridium acetobutylicum défectifs dans la production d'acétone.