Oxygen consumption of the chick embryo's respiratory organ, the chorioallantoic membrane.
A new technique based on stopping the chick embryo's blood circulation in the intact egg was used to measure in situ the chorioallantoic (CA) oxygen consumption, MCAO2, from incubation day 12 to 20. Total egg MO2, MTOTO2, and wet and dry masses of embryo and CA were also measured daily. Embryo MO2, MEMBO2, was calculated. Mean MCAO2 decreased from 71 mumol X h-1 (17% of MTOTO2, 24% of MEMBO2) at 12 days to 62 mumol X h-1 (5% of MTOTO2) at 20 days. Dry mass of CA did not change significantly. Water remained at a high level in CA (88-94%), but embryo water decreased from 93% to 82% between days 12 and 20. The fairly high level of MCAO2, more marked at young stages, calls for corrections in respiratory and circulatory embryonic variables derived from MTOTO2, such as CA blood flow, CA diffusive capacity for O2, and CA arterio-venous shunt. Mass specific values and intra-specific allometric relations in bird embryos should be recalculated on the basis of MEMBO2 instead of MTOTO2.
D000482:Allantois / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002642:Chick Embryo / Q000502:physiology*; D002823:Chorion / Q000378:metabolism*; D005321:Extraembryonic Membranes / Q000378:metabolism*; D010101:Oxygen Consumption; D012137:Respiratory System / Q000196:embryology* / Q000378:metabolism