title: Localisation of the protein and glycoprotein components of bovine nasal epithelial desmosomes by immunoelectron microscopy.
abstract: Desmosomal proteins (dp1-4) and glycoproteins (dg1-3) have been localised within desmosomes of bovine nasal epithelium by immunogold labelling of ultrathin frozen sections. Beginning in the extracellular space and proceeding through the plaque to the tonofilaments, the following localisations were found. Labelling for the 130,000 and 115,000 Mr glycoproteins (dg2 and dg3) was predominantly in the extracellular space, a location consistent with their proposed adhesive function. The glycoproteins of 175,000-164,000 Mr (dg1) were also found in the extracellular space and in addition had cytoplasmic domains extending throughout the cytoplasmic plaque. The 83,000 Mr protein (dp3) was located along the cytoplasmic face of the membrane and extended into the plaque, whereas an antibody which recognises both the 83,000 Mr protein (dp3) and the 75,000 Mr protein (dp4) gave labelling both in and beyond the plaque. Labelling for the high mol. wt proteins of Mr 250,000 and 215,000 (dp1 and dp2) was largely excluded from the plaque, and was located distally, adjacent to the tonofilaments. Hemidesmosomes could not be labelled with antibodies to dg1-3 or dp3 and 4, but some labelling was obtained with antibody to dp1 and 2.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D002417:Cattle; D003896:Desmosomes / Q000648:ultrastructure*; D004848:Epithelium / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis*; D007106:Immune Sera; D008854:Microscopy, Electron; D008970:Molecular Weight; D009297:Nasal Mucosa / Q000648:ultrastructure*; D011506:Proteins / Q000032:analysis*
chemical_list: D000936:Antigen-Antibody Complex; D006023:Glycoproteins; D007106:Immune Sera; D011506:Proteins