Covalent attachment of oligonucleotides to solid supports.
Coupling efficiencies for the covalent attachment of oligonucleotides (17-29 bases in length) to solid supports derivatized with alkyl-amino and -carboxylic functionalities have been determined. Attachment efficiencies of 60-80% were obtained for coated long-chain alkylamino controlled pore glass (CPG) supports. Similar efficiencies of immobilization were observed for carboxyl-bearing supports, which additionally exhibited lower levels of non-covalent binding. The extent of terminally linked oligonucleotide was determined to be 50-55% of the overall attachment in the carbodiimide-mediated coupling reaction of a 5'-aminohexyl phosphoramidate derivative of a 29-mer to Sephacryl carboxyl support. While lower overall efficiencies of attachment were obtained in the reaction with Sephacryl N-hydroxysuccinimide-activated carboxyl support, greater than 80% of this coupling results in end-attached oligonucleotides.
D055598:Chemical Phenomena; D002621:Chemistry; D005898:Glass; D007202:Indicators and Reagents; D009841:Oligonucleotides; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D012343:RNA, Transfer; D012116:Resins, Plant; D013604:T-Phages / Q000201:enzymology
D007202:Indicators and Reagents; D009841:Oligonucleotides; D012116:Resins, Plant; D012343:RNA, Transfer; D011116:Polynucleotide 5'-Hydroxyl-Kinase