Individual mouse VL30 elements transferred to rat cells by viral pseudotypes retain their responsiveness to activators of protein kinase C.
By exploiting the retroviral characteristics of mouse VL30 elements, we transferred individual copies of VL30 to Rat-1 cells by infection. Analysis of clonal isolates containing single VL30 elements integrated into the Rat-1 genome indicates that responsiveness to activators of protein kinase C is an inherent property of at least some of the VL30 sequences.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004789:Enzyme Activation; D051379:Mice; D011493:Protein Kinase C / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics; D051381:Rats; D012190:Retroviridae / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D004279:DNA, Viral; D012367:RNA, Viral; D011493:Protein Kinase C