title: Transacylase activity of lactating bovine mammary fatty acid synthase.
abstract: An assay for the transacylation reaction catalyzed by fatty acid synthase was developed which does not require model substrates or labelled acyl-derivatives of CoA. It involves the transfer of the acyl group from unlabelled CoA to [3H]CoA. This assay shows the occurrence of transacylation at a relatively high rate with a variety of substrates that the enzyme is able to utilize. The activity is unaffected by dissociation of the enzyme or modification by iodoacetamide or 2-chloroacetyl-CoA.
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism; D000818:Animals; D002417:Cattle; D064429:Fatty Acid Synthases / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D007774:Lactation; D008321:Mammary Glands, Animal / Q000201:enzymology*; D011247:Pregnancy; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; D000123:Acetyltransferases; D064429:Fatty Acid Synthases