Fracture labelling of boar spermatozoa for the fucose-binding-protein (FBP).
Labelling of fractured boar spermatozoa with the FUC-HRP gold method for a fucose-binding-protein (FBP) gave evidence the FBP is localized in the acrosomal matrix. All fracture faces through the acrosome from the rostral end towards the equatorial segment show similar labelling pattern. This labelling is completely blocked by preincubation of the fractured tissue with focoidan.
D000177:Acrosome / Q000032:analysis; D000818:Animals; D005614:Freeze Fracturing; D006046:Gold; D006735:Horseradish Peroxidase; D037102:Lectins / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000494:pharmacology; D013094:Spermatozoa / Q000032:analysis* / Q000648:ultrastructure; D013552:Swine
D037102:Lectins; D011134:Polysaccharides; C027112:fucose-binding lectin; D006046:Gold; C007789:fucoidan; D006735:Horseradish Peroxidase