A rapid and simple procedure for dissociation of tumor tissue from the human colon.
A rapid procedure for dissociation of colon tumor tissue which combines enzymatic and mechanical methods is described. Using this method a cell suspension almost free of cell aggregates is obtained which is further characterized by high cell yield and excellent cell viability.
D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000473:pathology*; D046508:Culture Techniques; D006801:Humans; D012004:Rectal Neoplasms / Q000473:pathology*