title: Mononuclear cell subsets in patients with oral cancer.
abstract: Peripheral mononuclear cells of ten patients with oral squamous cell carcinoma (SCC) and ten controls were evaluated for their cell subset composition. Flow cytometry experiments using commercially available subset-specific monoclonal antibodies (of the Leu series) were conducted to determine the percentage and fluorescent pattern of cell subsets. Leu-1+ (total T-cells), Leu-2a+ (T suppressor/cytotoxic cells), Leu-3a+ (T-helper/suppressor-inducer cells), Leu-7+ (natural killer cells), Leu-12+ (B-cells), and Leu-M3+ (monocytes) were analyzed. No significant difference was found between patients and controls in most instances; however, Leu-7+ cells were increased in patients (P less than 0.01). Preliminary experiments (and published evidence) suggest that this increase in Leu-7+ cells reflects an increase in natural killer cells, induced by a soluble factor released by squamous cell carcinoma cells.
mesh_terms: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D009062:Mouth Neoplasms / Q000276:immunology* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface