[The degree of modification of serum albumin as a toxicological test].
A procedure is developed, involving electrophoresis and TCA-ethanol treatment of electrophoregramms, which enabled to estimate quantitatively the modified derivatives of albumin just in blood serum without the protein isolation. The method is based on ability of native albumin to maintain its solubility after TCA-ethanol treatment. At the same time, modified albumin with conformational alterations was fixed on a carrier and might be estimated using colorimetry or densitometry. The procedure developed might be used in chemical industry for evaluation of toxic effects of some substances.
D002617:Chemical Industry; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D009784:Occupational Diseases / Q000097:blood* / Q000139:chemically induced; D012709:Serum Albumin / Q000032:analysis*
D012709:Serum Albumin
Stepen' modifikatsii syvorotochnogo al'bumina kak toksikologicheskiĭ test.