title: O2 radicals mediate reperfusion lung injury in ischemic O2-ventilated canine pulmonary lobe.
abstract: This study was undertaken to determine whether lung injury after a period of ischemia reperfusion is caused by O2 ventilation during ischemia and whether this injury is mediated by reactive O2 metabolites. Isolated canine left lower pulmonary lobes were subjected to room temperature ischemia for 6 h while being ventilated with either 100% O2, room air, or 100% N2. After the ischemic period, all lobes were perfused with autologous blood and ventilated with 100% O2 for an additional 4 h. In lobes ventilated with 100% O2 during the ischemic period, massive weight gain (228%) occurred 4 h after reperfusion. A marked increase in pulmonary shunt was noted. Lobes ventilated with room air behaved similarly. In contrast, lobes ventilated with 100% N2 gained significantly less weight (54%) and did not manifest any increase in pulmonary shunt. When lobes ventilated with 100% O2 or room air were pretreated with superoxide dismutase (SOD), the injury was significantly reduced. Pressure-volume deflation study of lobes, after ischemia only, demonstrated that ventilation with 100% O2 and with 100% N2 both equally decreased pulmonary compliance. We conclude that lung ischemia-reperfusion injury is related to O2 ventilation during ischemia and that injury can be prevented by administration of SOD or ventilation with 100% N2. This suggests that the injury is related to O2 metabolites produced during O2 ventilation in the absence of the circulation.
mesh_terms: D000818:Animals; D001794:Blood Pressure; D004285:Dogs; D007511:Ischemia / Q000503:physiopathology*; D008168:Lung / Q000098:blood supply* / Q000187:drug effects; D009929:Organ Size; D010100:Oxygen / Q000378:metabolism*; D011651:Pulmonary Artery / Q000503:physiopathology; D011652:Pulmonary Circulation; D011667:Pulmonary Veins / Q000503:physiopathology; D012039:Regional Blood Flow; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D013482:Superoxide Dismutase; D010100:Oxygen