title: One-tube radiotransinassay for determination of cortisol at ambient temperature.
abstract: After extraction of the sample (on filter paper) in the counting vial, cortisol is assayed by adding 50 microL of horse serum containing tritiated cortisol, and 2 mL of toluene scintillator, shaking for 20 min, and counting the radioactivity in a liquid-scintillation counter at ambient temperature. The method can be applied to saliva, serum, or urine--directly as wet samples or dried on the filter paper. Only inexpensive reagents, which are stable for months to years, are needed.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D005260:Female; D006736:Horses / Q000097:blood; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis* / Q000097:blood / Q000652:urine; D011869:Radioligand Assay; D012463:Saliva / Q000032:analysis; D014156:Transcortin / Q000378:metabolism
chemical_list: D014156:Transcortin; D006854:Hydrocortisone