Studies on the metabolism of deoxynivalenol in the rat.
The metabolism and tissue distribution of [14C]deoxynivalenol have been studied in male PVG rats. Following administration of a single oral 10-mg/kg dose, radioactivity excreted in the urine and faeces accounted, respectively, for 25 and 64% of the administered dose within 96 hr. Less than 0.15% of the dose was detected in the respired air. Very little radioactivity appeared to be retained in any of the tissues examined after 96 hr. HPLC separation of several urinary and faecal metabolites was achieved on a reversed-phase column, using two different elution systems, one at neutral pH and one acidified. Two of the major non-polar HPLC peaks were identified by gas chromatography-mass spectrometry as unchanged deoxynivalenol and 3 alpha,7 alpha,15-trihydroxytrichothec-9,12-dien-8-one.
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051381:Rats; D012717:Sesquiterpenes / Q000378:metabolism*; D014018:Tissue Distribution; D014255:Trichothecenes / Q000378:metabolism* / Q000652:urine
D012717:Sesquiterpenes; D014255:Trichothecenes; C007262:deoxynivalenol