Effect of different oxygen saturations on venous versus arterial endothelial cell growth in vitro.
Canine carotid arterial and external jugular venous endothelial cells were derived, cutured, passed, and pooled. Three X 10(6) cells were seeded into capped roller-bottles. Three roller-bottes were seeded with venous, and another three with arterial derived endothelial cells for each of three "oxygen saturations" 5, 20 and 50% O2 respectively, and incubated for seven days and then counted. Arterial endothelial cells showed no cell proliferation in high oxygen saturation compared to an 83% increase in cell numbers when oxygen supply had been held to 20%. Venous endothelial cells showed no cell proliferation in medium or high oxygen supply but low oxygen supply (5% O2) gave a 91% increase in cell counts. The small number of roller-bottles investigated does not allow firm conclusions but our results warrant further evaluation.
D000332:Aerobiosis; D000818:Animals; D002339:Carotid Arteries / Q000166:cytology*; D002455:Cell Division; D002478:Cells, Cultured; D004285:Dogs; D004727:Endothelium / Q000166:cytology*; D007601:Jugular Veins / Q000166:cytology*; D009928:Organ Specificity; D010313:Partial Pressure