Different patterns of testicular in vitro metabolism of [14C]testosterone in several Betta (Anabantoidei, Belontiidae) species.
Testicular tissues of Betta picta, Betta smaragdina, and the short-finned variety of Betta splendens were incubated with [14C]testosterone at 27 degrees for 120 min and the metabolites were isolated and characterized by paper and thin-layer chromatography and eventually by crystallization to constant specific activity. The metabolic profiles of the species were totally different. The short-finned B. splendens formed mainly 11-ketotestosterone (51.4%) as does the veiltail variety. B. smaragdina was the only species which formed considerable amounts of conjugates (24.3%), whereas in B. picta almost exclusively reduced (5 beta-) compounds (66.2%) were metabolites of testosterone. The results are discussed to be attributable to differences in testicular steroid metabolism. The significance of this observation remains unclear.
D000728:Androgens / Q000302:isolation & purification; D000818:Animals; D002250:Carbon Radioisotopes; D005399:Fishes / Q000378:metabolism*; D008297:Male; D013045:Species Specificity; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
D000728:Androgens; D002250:Carbon Radioisotopes; D013739:Testosterone