Experience with routine applications of liquid chromatography-mass spectrometry in the pharmaceutical industry.
The combination of liquid chromatography and mass spectrometry (LC-MS) has been established to complement gas chromatography (GC)-MS in the analysis of non-volatile and labile drugs in complex materials. The possibilities of LC-MS in the pharmaceutical industry for the analysis of drug substances and dosage forms, metabolism studies and the elucidation of the structures of materials of biological origin are discussed. Instrumental requirements, limitations and applications of LC-MS are considered and experiences with LC-MS in routine applications are reported. Preliminary results obtained with thermospray LC-MS are compared with those using a direct liquid inlet interface.
D000818:Animals; D001646:Bile / Q000032:analysis; D001971:Bromocriptine / Q000032:analysis; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D003524:Cyclosporins / Q000652:urine; D003984:Dibenzazepines / Q000032:analysis; D004345:Drug Industry; D006801:Humans; D013058:Mass Spectrometry; D009529:Nicardipine / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical
D003524:Cyclosporins; D003984:Dibenzazepines; D001971:Bromocriptine; D009529:Nicardipine; C041528:fluperlapine