[Binding of Ca2+ to glycoprotein from bovine heart mitochondria].
It is shown that glycoprotein from bovine heart mitochondria which forms Ca2+-selective conductance channels in a bilayer lipid membrane possesses Ca2+-binding activity. Ca2+-binding sites of two kinds were revealed in the glycoprotein molecule: high affinity sites with Kd = 2.8 X 10(-6) M and low affinity sites with Kd 1.1 X 10(-5) M. Ca2+-binding by the high affinity sites occurs co-operatively. The Hill coefficient is about 2.
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D006023:Glycoproteins / Q000378:metabolism*; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*
D006023:Glycoproteins; D002118:Calcium
Izuchenie sviazyvaniia Ca2+ glikoproteidom iz mitokhondriĭ serdtsa byka.