Ethidium bromide: destruction and decontamination of solutions.
Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution may be completely degraded by reaction with sodium nitrite and hypophosphorous acid. Only non-mutagenic reaction mixtures were produced. Destruction was greater than 99.8% in all cases; the limit of detection was 0.5 micrograms ethidium bromide per milliliter of solution. Ethidium bromide also may be removed completely from the above solutions by using Amberlite XAD-16 resin. The limit of detection was 0.05 micrograms ethidium bromide per milliliter of solution (0.27 micrograms/ml when cesium chloride solution was used).
D003666:Decontamination / Q000379:methods*; D004996:Ethidium / Q000633:toxicity; D009152:Mutagenicity Tests; D012722:Sewage; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014865:Waste Disposal, Fluid
D012722:Sewage; D012996:Solutions; D004996:Ethidium