[Flow cytometer (FCM) in biological characteristics of nasopharyngeal carcinoma xenografts in nude mice].
Tumor tissue from a patient with nasopharyngeal carcinoma was transplanted to nude mice (BALB/c) and had successfully been maintained through ten passages. The nude mice tumor and the primary human tumor were assayed by FCM for the DNA content in addition to conventional pathological examination, chromosome analysis and EBV assay. The tumor incidence in the different passages showed a marked change ranging from 25% to 81% with a mean value of 52% which tended to increase, however. The doubling time of six tumors in the ninth passage showed no great change 6-12 weeks after transplantation with a mean about 4.3 days. But at week 12, there was a significant difference between each tumor volume, ranging from 438 to 1,998 mm3. By FCM, it was found that the DNA index remained constant in both the primary and nude mice tumors. Among the nude mice tumors in different passages, the distribution of various phase cells in cell cycle was similar to that of the primary tumor. In conclusion, the use of FCM to assay the cellular DNA content in nude mice tumor is rapid and sensitive. It is helpful, for the nude mice tumor, in identifying stability of the biological characteristics and in studying of the mechanism of chemotherapy and radiotherapy in the future.
D000818:Animals; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D051379:Mice; D008807:Mice, Inbred BALB C; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D009368:Neoplasm Transplantation
D004273:DNA, Neoplasm