Optimal conditions for the measurement of lipid peroxidation products (lipofuscin) in individual cultivated human glial and glioma cells.
Age pigment (lipofuscin) has been quantified in individual cultivated human glial and glioma cells using fluorescent microscopic techniques and the effects of cell spreading after subcultivation, fixatives, mounting media and storage conditions on these measurements were investigated. The use of formalin fixation on cells that were permitted to spread at least 6 h after subcultivation, combined together with glycerol mounting media and subsequent storage at 4 degrees C provided the optimal versatility for lipofuscin quantification when multiparametric analysis of the cells was desired. Alternatively, Entellan was the mounting media of choice when long-term, non-multiparametrical studies are conducted and the cellular material cannot be stored below room temperature.
D000085:Acetates; D019342:Acetic Acid; D002460:Cell Line; D000431:Ethanol; D005470:Fluorometry; D005557:Formaldehyde; D005910:Glioma / Q000032:analysis*; D005990:Glycerol; D006801:Humans; D008062:Lipofuscin / Q000032:analysis*; D009457:Neuroglia / Q000032:analysis*; D010860:Pigments, Biological / Q000032:analysis*; D012117:Resins, Synthetic; D013696:Temperature
D000085:Acetates; D008062:Lipofuscin; D010860:Pigments, Biological; D012117:Resins, Synthetic; C052885:entellan; D005557:Formaldehyde; D000431:Ethanol; D005990:Glycerol; D019342:Acetic Acid