Diode-array spectrophotometry for simultaneous measurement of hemoglobin pigments.
A prototype oxygen saturation meter was used to measure the concentrations of deoxygenated hemoglobin (rHb), oxyhemoglobin (HbO2), carboxyhemoglobin (HbCO), methemoglobin (MetHb), and sulfhemoglobin (SHb) in 35 microliter blood. Simultaneous absorbance measurements at 535, 560, 577, 622, 636, and 670 nm permitted the composition of any hemoglobin pigment mixture to be determined more accurately, precisely and easily than before. The inclusion of 670 nm, where the hemoglobin pigments have low absorption coefficients, allowed correction for turbidity.
D000328:Adult; D002263:Carboxyhemoglobin / Q000032:analysis; D002849:Chromatography, Gas; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008706:Methemoglobin / Q000032:analysis; D009391:Nephelometry and Turbidimetry; D010100:Oxygen / Q000097:blood; D010108:Oxyhemoglobins / Q000032:analysis; D013053:Spectrophotometry / Q000295:instrumentation; D013435:Sulfhemoglobin / Q000032:analysis
D006454:Hemoglobins; D010108:Oxyhemoglobins; D008706:Methemoglobin; D013435:Sulfhemoglobin; D002263:Carboxyhemoglobin; D010100:Oxygen