Effect of parbendazole and piperazine adipate on the activity of some enzymes of Ascaridia galli and Heterakis gallinae.
Adult Ascaridia galli and Heterakis gallinae obtained from the fowl (Gallus gallus) were treated in vitro with 10(-2) to 10(-5) M parbendazole and piperazine adipate for 10-60 min at 38 degrees C. Both the compounds at 10(-2) M caused mortality of A. galli and H. gallinae after a maximum of 30 min exposure. The effect of the drugs on the homogenates of the treated worm was investigated. Parbendazole (10(-2) M) inhibited malate oxidation by 68% in A. galli and 62% in H. gallinae. Piperazine adipate (10(-2) M) inhibited malate oxidation by 78% in both parasites. In A. galli oxaloacetate reduction was inhibited by 41 and 26% by 10(-2) M parbendazole and piperazine adipate, respectively; with H. gallinae this inhibition was found to be 39 and 55%, respectively. Aldolase activity in both the parasites was also inhibited by 10(-2) M parbendazole and piperazine adipate. Both compounds caused an inhibition of acid phosphomonoesterase activity, but the activities of lactate dehydrogenase and alkaline phosphomonoesterase were not affected significantly. Parbendazole (10(-2) M) had no significant effect on the cholinesterase activity of these parasites, but piperazine adipate (10(-2) M) caused an inhibition of 96% in A. galli and 93% in H. gallinae. The possible mode of action of the drugs is discussed.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D001197:Ascaridia / Q000187:drug effects* / Q000201:enzymology; D001199:Ascaridoidea / Q000187:drug effects* / Q000201:enzymology; D001562:Benzimidazoles / Q000494:pharmacology*; D002802:Cholinesterases / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D000077489:Piperazine; D010879:Piperazines / Q000494:pharmacology*
D000969:Antinematodal Agents; D001562:Benzimidazoles; D010879:Piperazines; D000077489:Piperazine; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D002802:Cholinesterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005634:Fructose-Bisphosphate Aldolase; C100292:parbendazole