Deficient induction of sulfobromophthalein conjugating activity by phenobarbital in hamster liver.
Administration of phenobarbital, a known inducer of glutathione S-transferase activity in rat liver, failed to stimulate sulfobromophthalein (BSP) conjugation by liver cytosol in hamsters. The latter displayed poor ability to conjugate this substrate, despite very high glutathione-conjugating activity with the broad-spectrum substrate 1-chloro-2,4-dinitrobenzene (CDNB). Of the six substrates tested, in this species, 1,2-epoxy-3-(4-nitrophenoxy)propane (ENPP) was the only one whose conjugation was greatly enhanced by phenobarbital (+172%). Nevertheless, hamsters proved as responsive to phenobarbital induction as rats, since it increased their relative liver weight and microsomal enzyme activity. The deficient induction of liver BSP-conjugating activity observed with phenobarbital is consistent with the finding that it did not affect the hepatic transport of this substrate in hamsters.
D000818:Animals; D006224:Cricetinae; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004790:Enzyme Induction; D004852:Epoxy Compounds / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D019803:Glutathione Disulfide; D005982:Glutathione Transferase / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D009596:Nitrophenols / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D013448:Sulfobromophthalein / Q000378:metabolism*
D004137:Dinitrochlorobenzene; D004852:Epoxy Compounds; D009596:Nitrophenols; D013448:Sulfobromophthalein; C007606:1,2-epoxy-3-(p-nitrophenoxy)propane; D005982:Glutathione Transferase; D005978:Glutathione; D019803:Glutathione Disulfide; D010634:Phenobarbital