Crosslinking of DNA to nuclear lamina proteins by UV irradiation in vivo.
We have been able to demonstrate that a fraction of DNA becomes crosslinked to nuclear lamina shells isolated from Ehrlich ascites tumour cells irradiated with UV light. Terminal labeling of short DNA fragments covalently attached to proteins reveals that DNA has become crosslinked to all three lamins and to a protein comigrating with vimentin.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism / Q000528:radiation effects*; D007381:Intermediate Filament Proteins / Q000378:metabolism / Q000528:radiation effects; D034882:Lamins; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism / Q000528:radiation effects; D009698:Nucleoproteins / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D004273:DNA, Neoplasm; D007381:Intermediate Filament Proteins; D034882:Lamins; D009363:Neoplasm Proteins; D009698:Nucleoproteins