title: A comparison of three ELISA systems for the detection of Mycoplasma pulmonis antibody in rats.
abstract: Barrier maintained and conventionally reared rats were tested for the presence of antibody to M. pulmonis using three ELISA test systems. Agreement between the two commercial test kits was 100% for both the barrier maintained and conventionally reared rats. Agreement between the kits and the non-commercial ELISA was 96% for barrier maintained rats, but only 56% for conventionally reared rats. Procedural differences or the presence of reactive substances in the sera of conventionally reared rats could have accounted for the discrepancy between the non-commercial and commercial tests.
mesh_terms: D000818:Animals; D000830:Animals, Laboratory / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D009174:Mycoplasma / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000907:Antibodies, Bacterial