Interference in an electrochemical detection system for peroxidase-linked reactions based on a fluoride ion-selective electrode.
Use of the fluoride ion-selective electrode as a detector in a linked glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) method for glucose was investigated for possible interference from sugars, metabolites, drugs, and anticoagulants. The CV for the method was 1.5% (SD 0.37 mmol/L) at a glucose concentration of 25.0 mmol/L. Interference was studied with glucose at this concentration, interference being defined as any result differing by more than +/- 3 SD. When present in concentrations of clinical relevance, interference from urea, uric acid, or acetaminophen would preclude the use of this detection system for routine analysis.
D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D005947:Glucose / Q000032:analysis* / Q000592:standards; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D006801:Humans; D010088:Oxidoreductases; D010544:Peroxidases; D011786:Quality Control
D010088:Oxidoreductases; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D010544:Peroxidases; D005947:Glucose