title: Factors affecting production of coccoid forms by Campylobacter jejuni on solid media during incubation.
abstract: Conditions influencing the conversion of oxygen into toxic derivatives in media were investigated for their effects on production of coccoid forms in cultures of the enteric pathogen Campylobacter jejuni. Compared with stored media, production of coccoid forms was less on freshly prepared media. Whether freshly prepared or stored before use, brucella agar media produced the fewest coccoid forms under the test conditions. Addition of supplements used as detoxifying agents minimized production of these forms on media but antibiotic formulations used in selective media did not influence production of coccoid forms. Furthermore, the type of incubation atmosphere and the strain of C. jejuni influenced the proportions of coccoid forms in cultures. It was deduced from electron microscopy observations during prolonged incubation of cultures that the process of conversion to coccoid forms involves a loss of spiral morphology, a shortening of the cell and retraction of the cytoplasm towards a cell terminal region. Coccoid forms and some intermediate forms in thin sections were found to lack cell integrity. It is concluded that coccoid form production in cultures is a degenerate response to toxic oxygen derivatives in cultures.
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D002168:Campylobacter fetus / Q000166:cytology* / Q000254:growth & development / Q000648:ultrastructure; D003470:Culture Media; D006801:Humans; D008027:Light; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning
chemical_list: D003470:Culture Media