Autonomic nerve terminals in relation to contractile and non-contractile structures in the conduit coronary artery of the dog.
The ramus interventricularis anterior (RIA), its first- and second-order branch were prepared for EM (perfused with glutaraldehyde under pressure, or simply fixed with KMnO4). No nerve fibres were found in the tunica media of either of the three consecutive segments. In the tunica adventitia axons with varicosities were found at a distance from the tunica media of 0.5-15 microns (about 50% 0.5-4.5 microns) in the RIA, 0.4-12 microns (about 50% 0.5-3.4 microns) in the first-order branch and 0.3-6.0 microns (about 50% 0.3-2.3 microns) in the second-order branch. Varicosities contain small, dense-cored vesicles (35-60 nm) and large, dense-cored vesicles (70-90 nm, exceptionally up to 120 nm); the other type contains small, clear vesicles (35-60 nm) and few large, dense-cored vesicles (70-90 nm). The remarkably large distance between the nerve terminals and smooth muscle cells fits well with the small range of sympathetic control of the conduit coronary artery. Close apposition of nerve terminals to fibroblasts (30-200 nm) was revealed in all three consecutive coronary portions. Moreover, terminal axons often lose the Schwann cell cover on the abluminal site and face the fibroblast.
D000818:Animals; D001338:Autonomic Fibers, Postganglionic / Q000648:ultrastructure*; D003331:Coronary Vessels / Q000294:innervation* / Q000648:ultrastructure; D004285:Dogs / Q000033:anatomy & histology*; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000294:innervation* / Q000648:ultrastructure; D009411:Nerve Endings / Q000648:ultrastructure*