[Degradation of chromatin in thymus lymphocytes during inhibition of repair of spontaneous DNA damage].
Chromatin degradation and cell death were observed after 6-7 h incubation of mouse thymus lymphocytes with 1-beta-D-arabinofuranosylcytosine and hydroxyurea. The time dynamics of both processes was similar. In this case, just as after gamma-irradiation, nucleosomes and their oligomeres were the products of degradation. Puromycin and cycloheximide prevented the toxic action of DNA synthesis inhibitors on thymocytes. It is suggested that the accretion of unrepaired DNA damages to some critical level triggers the process of the internucleosome degradation of chromatin, i.e. implements the program of lymphocyte death.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D002843:Chromatin / Q000528:radiation effects*; D003561:Cytarabine / Q000633:toxicity; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D006918:Hydroxyurea / Q000633:toxicity; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D051379:Mice; D013950:Thymus Gland / Q000473:pathology / Q000528:radiation effects*
D002843:Chromatin; D003561:Cytarabine; D006918:Hydroxyurea
Degradatsiia khromatina v limfotsitakh timusa pri ingibirovanii reparatsii spontannykh povrezhdeniĭ DNK.