Capacitation-related changes in antigen distribution on mouse sperm heads and its relation to fertilization rate in vitro.
The anti-mouse sperm monoclonal antibody OBF13 did not react with fresh epididymal sperm. However, when sperm were incubated in a culture medium capable of inducing capacitation, the entire head of the sperm began to react with this antibody. This change of reactivity was not observed when sperm were incubated in a Ca2+-free medium. The change of the reactivity to the antibody was studied in relation to the fertilizing ability of sperm as measured in an in vitro fertilization system; a significant correlation was observed between the appearance of head-stained sperm and fertilization rate.
D000177:Acrosome / Q000502:physiology; D000818:Animals; D000941:Antigens / Q000032:analysis*; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013075:Sperm Capacitation; D013077:Sperm Head / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
D000941:Antigens