title: Purification, reconstitution and polymorphic transition of halobacterial flagella.
abstract: Flagellar filaments of Halobacterium halobium have been purified by dissociation and reconstitution. Three different protein bands (23,500, 26,500 and 31,500 apparent molecular weight) are seen on sodium dodecyl sulphate/polyacrylamide gels, thus confirming that all three proteins are intrinsic to the flagellar structure. We designate them as flagellin Fla I (23,500), Fla II (26,500) and Fla III (31,500). Polymorphic transitions from normal to a curly, a ring and a straight form are induced by different pH values and heat treatments.
mesh_terms: D005407:Flagella / Q000648:ultrastructure*; D005408:Flagellin / Q000302:isolation & purification; D006217:Halobacterium / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008970:Molecular Weight
chemical_list: D005408:Flagellin