Effect of elastin peptides on human monocytes: Ca2+ mobilization, stimulation of respiratory burst and enzyme secretion.
The effect of elastin peptides (Kappa-elastin) was investigated on human monocytes. The data presented here indicate that elastin peptides increase the intracellular Ca2+ level measured by Quin 2 fluorescence and mediate the release of beta glucuronidase and elastase. The O2 consumption and H2O2 release were stimulated in a dose-dependent manner. The early rise of cAMP was followed by a return to the original level at 30 min and by a concomitant increase of cGMP level. The action of elastin peptides on intracellular calcium level and cGMP levels may well be related to its previously demonstrated chemotactic activity. These activities may well play a role in the modifications of the extracellular matrix following elastin degradation as observed in atherosclerosis, emphysema and aging.
D002118:Calcium / Q000378:metabolism*; D004549:Elastin / Q000494:pharmacology*; D005089:Exocytosis / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009000:Monocytes / Q000502:physiology*; D009712:Nucleotides, Cyclic / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D010196:Pancreatic Elastase / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology
D009712:Nucleotides, Cyclic; D010446:Peptide Fragments; D004549:Elastin; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase; D010196:Pancreatic Elastase; D002118:Calcium