Identification of T-kininogen in rat urine.
Studies were carried out in order to characterize the kininogen in rat urine. Rat urine contained a component which was cross-reactive with antibody to rat plasma T-kininogen. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of rat urine revealed a single antigenic band corresponding to the molecular weight of plasma T-kininogen. Induction of acute inflammation in rats by an injection of lipopolysaccharide caused an increase in the urinary excretion of immunoreactive T-kininogen in parallel with an elevation of plasma T-kininogen. Kininogen partially purified from rat urine by affinity chromatography using S-carboxymethylated papain-agarose liberated only T-kinin upon trypsinization, but not upon treatment with rat glandular kallikreins. From these results, we conclude that T-kininogen is the major kininogen present in rat urine.
D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D007610:Kallikreins / Q000378:metabolism; D007704:Kininogens / Q000097:blood / Q000652:urine*; D007705:Kinins / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin / Q000378:metabolism
D007704:Kininogens; D007705:Kinins; D008070:Lipopolysaccharides; D007610:Kallikreins; D014357:Trypsin