title: Fetal expression of muscle-specific isoactins in multiple organs of the Wistar-Kyoto rat.
abstract: Actin, a cytoskeletal and contractile protein, is expressed in six different isoforms that exhibit striking specificity. No studies have considered the muscle-specific actin expression in multiple organ systems in the intact fetus. Using a monoclonal antibody (B4) which reacts specifically with the isoactins of the smooth and skeletal muscle our immunohistochemical study examined whole fetal body sections to follow the development of actin expression throughout the last third of gestation in the Wistar-Kyoto rat. B4 staining was exclusively localized to muscle, confirming its high specificity and its usefulness for studying the ontogeny of muscle-specific isoactins. At 15 days of gestation, B4 staining was detected in the heart, the thoracic aorta and the skeletal muscle of the chest wall. The distribution and intensity of staining in the heart were initially higher than in the aorta or skeletal muscle and remained unchanged throughout the remainder of gestation, suggesting that the maturation of cardiac actin expression is well developed, although not fully completed before birth. Expression of muscle-specific actins in skeletal muscle was age-dependent and correlated with the maturational changes of muscle cell precursors. B4 staining in the fetal kidney was not apparent until day 20 of gestation and was localized to the inner cortical vessels. in association with the most mature nephrons, suggesting a centrifugal maturation of the intrarenal vasculature. The intensity of B4 staining in most tissues including bronchi, bowel, diaphragm, chest wall muscle and peripheral and pulmonary arteries increased by the end of gestation.
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D004064:Digestive System / Q000196:embryology; D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D005865:Gestational Age; D006321:Heart / Q000196:embryology; D007668:Kidney / Q000196:embryology; D008168:Lung / Q000196:embryology; D009131:Muscle, Smooth, Vascular / Q000196:embryology; D009132:Muscles / Q000196:embryology*; D009928:Organ Specificity; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D000199:Actins