Monolayer system of cultivating bovine polymorphonuclear leukocytes (PMNL): effect of long-term cultivation on the viability and phagocytic function of the cells.
A monolayer system of cultivating bovine PMNL is described. Poly-L-Lysine (PLL) significantly (P less than 0.01) enhanced the attachment of bovine PMNL to glass. Long-term cultivation up to seven days, in a mixture of calf serum and RPMI 1640, had no effect on the viability of the cells, but cultivation for 3 days or more significantly reduced the ability of the PMNL to phagocytose bacteria.
D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D010587:Phagocytosis
D003470:Culture Media