Reversed-phase high-performance liquid chromatographic separation and electrochemical detection of retinol and its isomers.
Baseline separation of the isomers of retinol using reversed-phase high-performance liquid chromatography (HPLC) in less than 30 min is presented. A new approach to the detection of retinol using electrochemical detection is developed. The oxidative electrochemistry of retinol is studied at a glassy-carbon electrode using coulometry, ultraviolet-visible spectrophotometry and HPLC. Amperometric detection in HPLC for retinol provided a linear response from 0 to 1.5 micrograms/ml and a detection limit of 4.1 ng/ml. Electrochemical detection was compared to ultraviolet-visible absorbance detection for the determination of retinol in human serum extracts. Good agreement is found for the results obtained with the two detectors.
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014801:Vitamin A / Q000032:analysis* / Q000097:blood
D007202:Indicators and Reagents; D014801:Vitamin A