Glycoproteins in the whorls of membrane produced by oligodendroglia in culture.
Two glycoproteins of 99 kDa and 77 kDa which exhibit intense binding to wheat germ agglutinin have been purified from the whorls of membrane produced by oligodendroglia in culture. The whorls of membrane were isolated by gradient centrifugation from purified bovine oligodendroglia maintained in culture. The two glycoproteins were solubilized from the membranes using a non-ionic detergent and purified by Sephadex LH-60 chromatography, wheat germ agglutinin affinity chromatography, and SDS-polyacrylamide pore gradient gel electrophoresis. HPLC peptide mapping of the 99-kDa and 77-kDa glycoproteins revealed structural differences between the two proteins. Peptide mapping suggested that the 99-kDa glycoprotein from the whorls of membrane may be homologous to that from the plasma membranes. The 77-kDa glycoproteins from both sets of membrane may also be structurally related. Lectin binding studies showed that both glycoproteins from the whorls of membrane bound to wheat germ agglutinin, succinylated wheat germ agglutinin, concanavalin A, and lentil lectin, indicating the presence of high mannose and hybrid type oligosaccharide side-chains.
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis*; D002478:Cells, Cultured; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009439:Neuraminidase / Q000378:metabolism; D009457:Neuroglia / Q000648:ultrastructure*; D009836:Oligodendroglia / Q000648:ultrastructure*; D010446:Peptide Fragments / Q000302:isolation & purification
D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D009439:Neuraminidase; D002918:Chymotrypsin