Lipid specific penetration of melittin into phospholipid model membranes.
The relative depth of penetration of melittin into egg phosphatidylcholine and bovine heart cardiolipin model membranes was investigated using fluorescence spectroscopy techniques. The tryptophan intrinsic fluorescence shift suggests a more hydrophobic surrounding of this residue in cardiolipin, while the accessibility for charged and uncharged aqueous quenchers is decreased in the cardiolipin system when compared with the phosphatidylcholine-bound situation. A lipid incorporated hydrophobic, collisional quencher and a resonance energy transfer acceptor on the other hand are more effective in quenching the tryptophan fluorescence of cardiolipin bound melittin. The combination of these results is interpreted as prove of a deeper positioning of the tryptophan containing part of the peptide molecule in the cardiolipin system in comparison with the situation in phosphatidylcholine. Models that take this difference into account are presented, which try to explain the opposite effect of melittin binding to the two lipid systems with respect to supramolecular structure, as reported in the preceding article (Batenburg, A.M., Hibbeln, J.C.L., Verkleij, A.J. and De Kruijff, B. (1987) Biochim. Biophys. Acta 903, 142-154).
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000838:Anions; D000873:Anthracenes; D001514:Bee Venoms / Q000378:metabolism*; D002417:Cattle; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004735:Energy Transfer; D005453:Fluorescence; D008081:Liposomes / Q000378:metabolism*; D008555:Melitten / Q000302:isolation & purification / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D009206:Myocardium / Q000032:analysis; D009566:Nitrates; D010713:Phosphatidylcholines / Q000378:metabolism; D010741:Phospholipases A; D010743:Phospholipids / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013229:Stearic Acids; D014364:Tryptophan
D000596:Amino Acids; D000838:Anions; D000873:Anthracenes; D001514:Bee Venoms; D008081:Liposomes; D008563:Membrane Lipids; D009566:Nitrates; D010713:Phosphatidylcholines; D010743:Phospholipids; D013229:Stearic Acids; D008555:Melitten; D014364:Tryptophan; C053687:6,7-dibromostearic acid; D010741:Phospholipases A; C034020:anthracene