Brugia pahangi: inhibition of protein synthesis.
The effects of inhibitors of protein synthesis and electron transport on the incorporation of [14C]leucine and [35S]methionine into protein by the filarial worm Brugia pahangi have been investigated. Cycloheximide inhibits the accumulation of both [14C]leucine and [35S]methionine by the worms and their incorporation into protein. In addition, inhibitors of electron transport and some anti-parasitic compounds also significantly inhibit filarial protein synthesis. Antimycin A and cyanide inhibit [14C]leucine incorporation into protein 63 and 72%, respectively, without affecting either motility or lactate production. Interestingly, the anti-malarial compounds chloroquine and quinacrine also significantly inhibit both accumulation and incorporation of [14C]leucine by B. pahangi. In addition, fluorographs of sodium dodecyl sulfate-polyacrylamide gels of homogenates from filariids incubated in [35S]methionine and cycloheximide with and without chloramphenicol indicate that there is a discrete population of proteins, possibly mitochondrial in origin, that are synthesized in the presence of cycloheximide and are not inhibited by chloramphenicol.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002009:Brugia / Q000187:drug effects*; D002701:Chloramphenicol / Q000494:pharmacology; D002738:Chloroquine / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects*; D007773:Lactates / Q000096:biosynthesis; D008970:Molecular Weight; D009068:Movement / Q000187:drug effects; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011796:Quinacrine / Q000494:pharmacology
D003486:Cyanides; D007773:Lactates; D011500:Protein Synthesis Inhibitors; D000968:Antimycin A; D002701:Chloramphenicol; D002738:Chloroquine; D003513:Cycloheximide; D011796:Quinacrine