[Excision of the plasmid containing the inserted human satellite DNA from Chinese hamster chromosomes after fusion of hamster and human cells].
Fusion of the Chinese hamster stably transformed Tk+ cells containing chromosomally integrated plasmid with a fragment of human satellite III DNA (HS3), with mitomycin C-treated human cells (B-lymphoma, line Raji) results in amplification and excision of heterogeneous plasmid material from hamster chromosomes. Some of these plasmids contain HS3. Functional activity of HS3 in the initiation of DNA replication is shown in transient transfection experiments. The results indicate that mitomycin C induces in human cells some trans-acting factors (possibly proteins) activating a replication origin within HS3 DNA and leading to plasmid replication in situ and their excision from chromosomes.
D000818:Animals; D002459:Cell Fusion; D006224:Cricetinae; D003412:Cricetulus; D004276:DNA, Satellite / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012093:Replicon; D014170:Transformation, Genetic
D004276:DNA, Satellite
Ekstsiziia plazmidy, soderzhashcheĭ vstavku satellitnoĭ DNK cheloveka, iz khromosom kletok kitaĭskogo khomiachka pri sliianii kletok khomiachka s kletkami cheloveka.