The effect of 2,5-hexanedione on newly formed collagen in granulation tissue.
The mechanical strength of newly formed collagen in granulation tissue was investigated by implantation of cellulose sponges into rats intoxicated with 2,5-hexanedione (2,5-HD) and control rats. Intoxication with 2,5-HD caused a significant decrease in mechanical strength after 20 days of implantation. No differences were found between 2,5-HD intoxicated and control groups in the amount of collagen formed in the sponges. The mechanical strength of granulation tissue depends on formation and cross-linking of the newly formed collagen fibrils. 2,5-HD is known to react with epsilon-amino groups of lysine and it is therefore able to interfere with both fibril aggregation and cross-linking of the collagen. On the other hand, 2,5-HD induces the formation of cross-links in intact tendon collagen. Therefore, we stress that it is essential to distinguish between intact and newly formed fibrils when evaluating the effect of 2,5-HD on fibillary proteins.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003094:Collagen / Q000378:metabolism*; D006097:Granulation Tissue / Q000187:drug effects / Q000378:metabolism*; D006588:Hexanones / Q000494:pharmacology*; D007659:Ketones / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013718:Tensile Strength
D006588:Hexanones; D007659:Ketones; D003094:Collagen; C011269:2,5-hexanedione