title: [Flavonoids extracted from Ribes nigrum L. and Alchemilla vulgaris L.: 1. In vitro inhibitory activities on elastase, trypsin and chymotrypsin. 2. Angioprotective activities compared in vivo].
abstract: In vitro experiments were conducted on the inhibitory properties of extracts from Ribes nigrum L. and Alchemilla vulgaris L. (fractions A1 + A2, A1, A2) on activity of the proteolytic enzymes elastase, trypsin and alpha-chymotrypsin. Extracts from Ribes Nigrum L. and Alchemilla Vulgaris L. (Fraction A1) inhibited 50% of the activity of porcine pancreas elastase at concentrations of 0.56 mg/ml and 0.16 mg/ml, respectively, against a synthetic substrate. Inhibition was less effective on activity of trypsin and alpha-chymotrypsin. Marked in vivo angioprotective properties were shown by the compounds studied, except Fraction A2 of Alchemilla vulgaris L. which had no significant activity. The results suggest a possible role by these inhibitors in the protection of conjunctive and elastic tissues adversely affected by proteolytic enzymes. An additional advantage is their lack of toxicity.
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000187:drug effects; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D005419:Flavonoids / Q000494:pharmacology*; D066298:In Vitro Techniques; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D011480:Protease Inhibitors / Q000494:pharmacology*; D013552:Swine; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D002918:Chymotrypsin; D010196:Pancreatic Elastase
vernacular_title: Flavonoïdes extraits de Ribes nigrum L. et d'Alchemilla vulgaris L.: 1. Activités inhibitrices vis-à-vis de l'élastase, de la trypsine et de l'alpha-chymotrypsine in vitro. 2. Activités angioprotectrices comparées in vivo.