Enhancement of survival and proliferation of clonogenic cells by monocyte-derived growth factors.
Human peripheral blood monocytes release a factor that enhances the clonal growth of human epithelial tumor cells in soft agar. The monocyte-derived growth factor was needed for both cellular proliferation and survival. Survival of SW-13 colony-forming cells decreased linearly in the absence of monocyte-conditioned media (MO-CM). Cells failed to respond to MO-CM after four days in culture. Although MO-CM enhanced growth when cells were plated at low density, growth was also enhanced when cell density was not a limiting factor. MO-CM increased DNA synthesis of SW-13 cells growing in monolayer culture as measured by tritiated-thymidine incorporation. These findings support evidence indicating inflammatory products may play a role in maintenance of the transformed phenotype.
D000306:Adrenal Cortex Neoplasms / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D002470:Cell Survival; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects*; D006133:Growth Substances / Q000096:biosynthesis / Q000494:pharmacology*; D006801:Humans; D009000:Monocytes / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014410:Tumor Stem Cell Assay
D006133:Growth Substances; D013936:Thymidine