Growth of murine bone marrow under various oxygen conditions in media buffered with HEPES.
Increased plating efficiencies were observed for marrow granulocyte-macrophage precursor cells (CFU-gm) cultured in an experimental system at reduced gas-phase oxygen concentrations. However, only a small component of this increase was attributable to decreased oxygen. The changed media buffering system was the more dominant factor contributing to increased plating efficiency, suggesting that other factors in addition to a reduction in oxygen may influence clonogenicity.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000166:cytology; D006531:HEPES; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008264:Macrophages / Q000166:cytology; D051379:Mice; D010100:Oxygen
D006531:HEPES; D010100:Oxygen