Aluminium induced damage of the lysosomes in the liver, spleen and kidneys of rats.
The influence of repeated aluminium (Al) administration (0.05 or 0.5 mg 100 g-1 b.w.t. i.p. 5 times weekly for 12 weeks) on the lysosomal enzymes N-acetyl-beta-D-glucosaminidase (beta-NAG) and beta-glucuronidase (beta-Gluc) in serum, liver, spleen and kidneys of adult female rats with intact kidneys, (NR), or following partial nephrectomy (5/6 NX) was investigated. After A1 loading, at the high dose only, the beta-NAG in serum and the free beta-NAG in liver, spleen and kidneys increased. Latent beta-NAG levels decreased in all three organs the effect being dose related. Following A1 loading no elevation in total enzyme activity was observed, with one exception. Depending on A1 doses the spleen of the non-operated animals, the liver of both groups of animals and the serum showed a decrease in beta-Gluc activity. No effect on beta-Gluc activity was observed in the spleen of 5/6 NX animals or in the kidneys of either group of animals. The results confirm that high doses of Al induce toxic effects and damage the lysosomes in the liver, the spleen and the kidneys. The results indicate that the extent of lysosomal damage correlates with dose and duration of Al loading. Repeated administration of Al also interferes selectively with enzyme synthesis.
D000117:Acetylglucosamine / Q000378:metabolism; D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D004798:Enzymes / Q000097:blood; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000648:ultrastructure; D007676:Kidney Failure, Chronic / Q000503:physiopathology; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008247:Lysosomes / Q000187:drug effects*; D010783:Photometry; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000187:drug effects / Q000648:ultrastructure
D004798:Enzymes; D000535:Aluminum; D005966:Glucuronidase; D000117:Acetylglucosamine