Malonate transport in human red blood cells.
Kinetic parameters of [2-14C]malonate uptake by the human erythrocyte membrane have been determined as Km, 24 mM and turnover number, 5 X 10(4) s-1. The translocation of this organic dianion is concentration, pH and temperature dependent. Competitive inhibition of malonate uptake by eosin and inorganic anions, strongly implies that a common route exists for both inorganic anions and organic dianions, namely the anion-exchange Band 3 protein. 14C-Malonate which is nonmetabolized in the erythrocyte, could be a useful probe for monitoring anion-exchange in reconstituted Band 3 systems.
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism; D001693:Biological Transport, Active / Q000187:drug effects; D003998:Dicarboxylic Acids / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007474:Ion Exchange; D007700:Kinetics; D008314:Malonates / Q000097:blood*
D001457:Anion Exchange Protein 1, Erythrocyte; D003998:Dicarboxylic Acids; D008314:Malonates; C030290:malonic acid