Biochemistry of packed red blood cell concentrates stored in PAGGS-sorbitol solution for 42 days.
We investigated metabolic, blood gas and acid-base balance modifications of erythrocyte concentrates resuspended in PAGGS-sorbitol solution during 6 weeks of storage. Glucose utilization was impaired during the last 2 weeks, while the intraerythrocytic ATP level decreased only 50% from the 1st to the 6th week. The K+ and Hb concentration in the medium showed a progressive increase which was more pronounced during the last 2 weeks. The decrease in pH progressed to reach 6.27 at the 6th week of storage. Intraerythrocytic 2,3-DPG dropped rapidly in the course of the 1st week and the percentage of oxyhemoglobin showed a remarkable increase after the 1st week (90%). Our data suggest that erythrocyte concentrates in PAGGS-sorbitol should be transfused, for optimal efficacy, within 4 weeks.
D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000032:analysis; D001793:Blood Preservation / Q000379:methods*; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D006151:Guanosine / Q000494:pharmacology; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology*
D006454:Hemoglobins; C053620:PAGGS-sorbitol solution; D010710:Phosphates; D006151:Guanosine; D012965:Sodium Chloride; D013012:Sorbitol; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine; D011188:Potassium