title: Effect of simulated hypoxia (7620 m, 6 h) on hepatic microsomal drug metabolising enzymes activity in rats given ethanol and vitamin A.
abstract: The hepatic MFO activity has been studied in rats exposed to acute hypoxia given single dose of ethanol (3 g/kg body weight) alone or along with 10,000 I.U. of vitamin A. The cytochrome P-450 was found to be depressed on ethanol administration in rats. Hypoxic exposure of rats given ethanol with or without vitamin A, however, restored the depressed cytochrome P-450 activity. Cytochrome-c-reductase, on the other hand, was increased on administration of ethanol with or without vitamin A supplementation. Exposure to hypoxia did not have any additional effect on cytochrome c-reductase activity. Aminopyrine-N-demethylase was depressed on ethanol administration. Vitamin A administration further depressed this enzyme. Hypoxic exposure, however, did not restore this enzyme level. Aniline hydroxylase was not affected by ethanol administration, vitamin A supplementation and hypoxic exposure. Thus, mixed function oxidase system appears to be differently affected on ethanol administration, vitamin A supplementation and hypoxic exposure suggesting that these effectors have different loci of interaction in microsomal structure and/or in its micromileau of cofactors.
mesh_terms: D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D000860:Hypoxia / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014801:Vitamin A / Q000494:pharmacology*
chemical_list: D014801:Vitamin A; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D009245:NADH Dehydrogenase