title: Comparison of Dermatophagoides pteronyssinus allergens from culture medium extract and whole body extract by using the same probe of pooled human serum.
abstract: Two distinct allergens of Dermatophagoides pteronyssinus were isolated from culture medium extract and whole body extract monitored by the same probe of pooled human serum and compared. IgE-binding activity of allergens from the two sources was detected by microplate-ELISA with the same probe throughout the column chromatography. An allergen obtained from culture medium extract (Ag-CME) had a molecular weight of 27,000 by SDS-polyacrylamide gel electrophoresis (PAGE) and two isoelectric point ranges, pI 4.0-4.7 and 5.9-6.8. Another allergen from whole body extract (Ag-WBE) had a molecular weight of 15,000 by SDS-PAGE and an isoelectric point range, pI 4.8-5.3 Ag-CME and Ag-WBE are probably the same allergens as antigen P1 and DpX, respectively.
mesh_terms: D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007525:Isoelectric Focusing; D008925:Mites / Q000276:immunology*; D008970:Molecular Weight
chemical_list: D000485:Allergens; D003470:Culture Media; D007073:Immunoglobulin E