Marked changes in red cell volume response to isotonic glucose solution during storage.
The changes of erythrocyte-volume caused by isotonic hexose solutions, glucose and galactose, during storage were studied. Blood was stored for 13 weeks at 4 degrees C. The determinations were carried out using a newly developed blood cell volume analyzer which can measure the continuous changes in cell volume. The erythrocyte volume gradually increased and reached a plateau after 13 weeks of storage. The percent volume increase in hexose solutions markedly decreased during the first 4 weeks of storage. These changes may have been due to denaturation of the membrane protein responsible for hexose permeation during storage.
D001793:Blood Preservation; D002463:Cell Membrane Permeability; D004909:Erythrocyte Indices / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D013997:Time Factors
D007552:Isotonic Solutions; D005947:Glucose