Electrochemistry in vivo: monitoring dopamine release in the brain of the conscious, freely moving rat.
Changes in electrochemical responses at stearate-modified and unmodified graphite paste electrodes were compared simultaneously by intrastriatal chronoamperometry in freely moving rats. Responses at the modified electrodes were unaffected by ascorbate administration, decreased by pergolide and increased by pargyline and haloperidol. The effects of haloperidol were reversed by gamma-butyrolactone but not by pargyline. In contrast, responses at the unmodified electrodes were increased by ascorbate or pergolide and decreased by pargyline. Haloperidol-induced increases at these electrodes were rapidly reversed by pargyline. Responses of the two electrodes differed significantly in both magnitude and temporal characteristics after amphetamine administration. The results demonstrate that the modified electrodes can selectively monitor released dopamine in the freely moving animal, even when there are simultaneous, large changes in ascorbate and 3,4-dihydroxyphenylacetic acid.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003913:Dextroamphetamine / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006108:Graphite; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D010293:Pargyline / Q000494:pharmacology; D010479:Pergolide / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D010479:Pergolide; D006108:Graphite; D010293:Pargyline; D006220:Haloperidol; D001205:Ascorbic Acid; D003913:Dextroamphetamine; D004298:Dopamine