title: Melittin induces HII phase formation in cardiolipin model membranes.
abstract: The interaction of melittin with bovine heart cardiolipin model membranes was investigated via binding assays, 31P-NMR, freeze-fracture electron microscopy, small angle X-ray diffraction and fluorescence based fusion assays. A strong binding (Kd less than 10(-7) M) appeared to be accompanied by the formation of large structures, resulting from a fusion process of extremely fast initial rate. As the melittin content is increased, bilayer structure is gradually lost and from a cardiolipin to melittin ratio of about 6 the lipid starts to organize itself in an hexagonal HII phase. At lower temperatures (T less than 40 degrees C) the coexistence of another structure is observed, characterized by a broad isotropic 31P-NMR signal and giving rise to sharp X-ray reflections, most probably a cubic phase, as suggested also be freeze-fracture images, showing orderly stacked particles. The results are discussed in relation to contrasting observations on the structural changes induced by melittin in the zwitterionic phospholipid system of dipalmitoylphosphatidylcholine (Dufourcq. J. et al. (1986) Biochim. Biophys. Acta 859, 33-48). The biological relevance of the observations with respect to the process of protein insertion into membranes is indicated.
mesh_terms: D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D002308:Cardiolipins / Q000378:metabolism*; D002417:Cattle; D005614:Freeze Fracturing; D008051:Lipid Bilayers / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008555:Melitten / Q000378:metabolism / Q000494:pharmacology*; D008561:Membrane Fusion / Q000187:drug effects; D008854:Microscopy, Electron; D009206:Myocardium / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D001514:Bee Venoms; D002308:Cardiolipins; D008051:Lipid Bilayers; D008081:Liposomes; D008555:Melitten