Enzyme immunoassay for detection of Mycoplasma bovis antigens in bull semen and preputial washings.
A capture enzyme immunoassay was developed for the detection of Mycoplasma bovis antigens in bull semen or preputial washings. IgG prepared from rabbits immunised with M bovis was passively adsorped to 96-well polystyrene plates. This antibody captured M bovis antigens which were then detected by using an IgG preparation from an immunised cow and murine monoclonal antibody to the bovine L-chain conjugated with horseradish peroxidase. The sensitivity of the assay was approximately 200 colour changing units (ccu)/ml and the specificity was excellent in that other species of mycoplasma, ureaplasma or acholeplasma did not react. A blind study of bull semen experimentally contaminated with M bovis detected all specimens with more than 200 ccu/ml.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D010413:Penis; D012661:Semen / Q000276:immunology*
D000942:Antigens, Bacterial