title: Conservation of plasmids among plant-pathogenic Pseudomonas syringae isolates of diverse origins.
abstract: Thirty isolates of Pseudomonas syringae pv. tabaci, pv. angulata (pathogens on tobacco), pv. coronafaciens, and pv. striafaciens (pathogens on oats) were examined for plasmid DNAs. The strains were obtained from plants throughout the world, some over 50 years ago. Of the 22 tobacco pathogens, 16 contain predominantly one type of plasmid, the pJP27.00 type. The remaining six tobacco-specific strains do not harbor detectable plasmids. The oat pathogens contain one, two, or three plasmids. DNA homology studies indicate that the plasmid DNAs are highly conserved. More importantly, the plasmids harbored by strains isolated from one host plant are conserved most stringently; e.g., the plasmids from the tobacco pathogens are, with one exception, indistinguishable by restriction endonuclease digestion and Southern hybridization. There is also extensive homology among plasmids indigenous to the oat-specific P. syringae pv. coronafaciens and pv. striafaciens strains.
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D002523:Edible Grain / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000382:microbiology*; D010947:Plants, Toxic; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity; D014026:Nicotiana / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial