Identification of a modified nucleoside located in the first position of the anticodon of Torulopsis utilis tRNAPro as 5-carbamoylmethyluridine.
An unknown nucleoside in the first position of the anticodon of Torulopsis utilis tRNAPro has been isolated. The UV, 1H NMR and secondary ion mass spectra indicated that this nucleoside is a uridine derivative, 5-carbamoylmethyluridine. The structure was completely established by comparison of the instrumental analysis results and chromatographic behavior of the isolated nucleoside with those of a synthetic sample.
D000926:Anticodon / Q000032:analysis*; D002175:Candida / Q000032:analysis*; D002845:Chromatography; D012331:RNA, Fungal / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*; D013057:Spectrum Analysis; D014529:Uridine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D000926:Anticodon; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; C030690:5-carbamoylmethyluridine; D012343:RNA, Transfer; D014529:Uridine