title: Receptors for lactogenic hormones in the porcine corpus luteum: properties and luteal phase concentrations.
abstract: Homogenates of pig corpora lutea contained specific, high-affinity receptors for ovine prolactin (oPRL) and human GH (hGH). Specific hormone binding was enhanced by divalent metal ions, but only when included in the binding reaction. Divalent metal ions did not act by increasing the recovery of bound hormone by low-speed centrifugation, but appeared to promote the formation of a more stable hormone-receptor complex. Both oPRL and hGH tracers were bound in similar amounts and with similar affinities by pig luteal homogenates and the concentrations of either unlabelled hormone required to displace specific binding of either tracer by 50% were identical. In contrast, 125I-labelled oGH failed to bind to pig luteal homogenates and oGH competed poorly for hGH or oPRL binding. Only hormones with prolactin-like activity competed for 125I-labelled oPRL binding. Specific prolactin binding was low in recently ovulated and early luteal phase corpora lutea, increased significantly in the mid-luteal phase and declined once more in the late luteal phase. Receptor concentrations increased with increasing gestational age.
mesh_terms: D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D013006:Growth Hormone / Q000378:metabolism; D011388:Prolactin / Q000302:isolation & purification / Q000378:metabolism; D011981:Receptors, Prolactin / Q000187:drug effects / Q000378:metabolism*; D011986:Receptors, Somatotropin / Q000378:metabolism; D013552:Swine
chemical_list: D002413:Cations, Divalent; D002614:Chelating Agents; D011981:Receptors, Prolactin; D011986:Receptors, Somatotropin; D011388:Prolactin; D013006:Growth Hormone