Reversal of cerulenin-induced inhibition of phospholipids and sterol synthesis by exogenous fatty acids/sterols in Epidermophyton floccosum.
Cerulenin, a specific inhibitor of fatty acids and sterol biosynthesis inhibited the growth of Epidermophyton floccosum, which was reversed when growth medium was supplemented with palmitic acid and sterols. Unsaturated fatty acids partially restored the growth. Cerulenin inhibited both phospholipid and sterol biosynthesis (60-70%) at the minimum inhibitory concentration (0.5 microgram/ml) as demonstrated by [32P]orthophosphoric acid and [14C]acetate incorporation into the respective lipids. Cerulenin-induced inhibition of phospholipid and sterol synthesis was dose dependent up to 0.5 microgram/ml. Exogenously supplied fatty acids and sterols restored the biosynthesis of phospholipids in cerulenin-treated cultures, while that of sterols was enhanced. The biosynthesis of both saturated and unsaturated fatty acids was inhibited by cerulenin.
D000085:Acetates / Q000378:metabolism; D000935:Antifungal Agents / Q000494:pharmacology*; D002569:Cerulenin / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology; D004821:Epidermophyton / Q000187:drug effects / Q000378:metabolism*; D004875:Ergosterol / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D010743:Phospholipids / Q000096:biosynthesis*; D010756:Phosphoric Acids / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis*
D000085:Acetates; D000935:Antifungal Agents; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010169:Palmitic Acids; D010743:Phospholipids; D010756:Phosphoric Acids; D013261:Sterols; D002569:Cerulenin; D019308:Palmitic Acid; D002784:Cholesterol; C030242:phosphoric acid; D004875:Ergosterol