Arachidonate epoxygenase: identification of epoxyeicosatrienoic acids in rabbit kidney.
Epoxyeicosatrienoic acids were isolated and purified from female rabbit kidneys. They were identified as a group, prior to resolution, by packed column gas-liquid chromatography-mass spectroscopic techniques as their methyl esters as well as their trimethylsilyl bromohydrin methyl esters. Initial capillary gas-liquid chromatography-mass spectral analysis of the corresponding hydrogenated pentafluorobenzyl esters revealed the presence of the 8,9- and 14,15-epoxyeicosatrienoate regioisomers. These results, in conjunction with the documented in vitro biological activities of the arachidonate epoxygenase metabolites, suggest a role for them in renal function.
D000818:Animals; D001095:Arachidonic Acids / Q000302:isolation & purification* / Q000378:metabolism; D002849:Chromatography, Gas; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System; D004852:Epoxy Compounds / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism*; D051381:Rats; D013696:Temperature
D001095:Arachidonic Acids; D004852:Epoxy Compounds; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D000090762:Cytochrome P-450 CYP2J2