title: Growth requirements and growth pattern of human urothelial cell lines of different grades of transformation.
abstract: Human urothelial cell lines propagated in culture have been classified into three grades of transformation (TGr I-III). This classification is unrelated to the histological classification of the original biopsy material. Mortal TGr I cell lines differed from immortal and nontumorigenic TGr II cell lines and tumorigenic TGr III cell lines; they have a lower growth rate, growth fraction, and saturation density, and a higher requirement for Ca2+. Except for one TGr II cell line, TGr II cells differed from TGr III cells by having a higher serum requirement and a lower saturation density. The ability to grow in agar was not correlated with the TGr of the cell lines.
mesh_terms: D001790:Blood Physiological Phenomena; D002118:Calcium / Q000494:pharmacology; D002295:Carcinoma, Transitional Cell / Q000473:pathology; D002452:Cell Count; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003470:Culture Media; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D014513:Ureter / Q000473:pathology*; D001743:Urinary Bladder / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000473:pathology
chemical_list: D003470:Culture Media; D006133:Growth Substances; D002118:Calcium