title: Factors affecting the secretion of submandibular salivary kallikrein in cats.
abstract: Glandular kallikrein has been assessed in submandibular saliva, homogenates and plasma by the fluorimetric substrate D-Val-Leu-Arg-7-amino-4-trifluoromethylcoumarin (AFC) and histochemically in tissue sections by the 4-methoxy-2-naphthylamide (MNA) analogue. Nerve stimulation was used to produce salivary secretion. Parasympathetic saliva contained low concentrations of kallikrein, independently of any circulating catecholamines from the adrenals. Sympathetic saliva contained very high concentrations of kallikrein; the amounts in individual drops rapidly reached a peak then declined gradually. Adrenergic blocking drugs during mixed parasympathetic and sympathetic stimulation showed that beta-adrenergic effects normally increase the secretion of kallikrein in response to the alpha-adrenergic influence from sympathetic nerve impulses. Small amounts of a glandular kallikrein-like activity are present in the plasma. Effluent blood from the submandibular gland before, during and after stimulation of either nerve gave no indication that submandibular kallikrein passes from the glandular compartment to the blood under conditions of unobstructed salivary flow. Excision of the chorda tympani indicated that parasympathetic nerve impulses are required for the normal resynthesis of submandibular kallikrein. The secretion of salivary kallikrein is essentially an exocrine function but its role in the saliva remains obscure. The results suggest that sudden mobilization of kallikrein may occur at times into the saliva and that a separate population of adrenergic axons, under separate central control, may pass to the striated ducts specially for this purpose.
mesh_terms: D000818:Animals; D002415:Cats; D002814:Chorda Tympani Nerve; D004087:Dihydroergotamine / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D006651:Histocytochemistry; D007610:Kallikreins / Q000097:blood / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010275:Parasympathetic Nervous System / Q000502:physiology; D011433:Propranolol / Q000494:pharmacology; D012463:Saliva / Q000378:metabolism*; D013363:Submandibular Gland / Q000294:innervation / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000502:physiology
chemical_list: D004087:Dihydroergotamine; D011433:Propranolol; D007610:Kallikreins