Separation of carbohydrates and lectins on carbohydrate-immobilized resins.
A few carbohydrate-immobilized resins were prepared by coupling glycamines of mono- and disaccharides to an epoxy-activated methacrylate base, and the elution profiles of various carbohydrates and lectins from columns packed with the resultant resins were compared. The introduction of glucamine, maltamine or lactamine to the methacrylate base had little effect on the separation of stereoisomers of aldoses, though it enabled group separation of pentoses as well as 6-deoxyhexoses from hexoses. On the other hand, the maltamine-immobilized resin showed strong specific affinity for concanavalin A, as well as lentil and pea lectins. The lactamine-immobilized resin was specific to ricinus agglutinin, peanut agglutinin and soy bean agglutinin. These carbohydrate-immobilized resins were also useful for the isolation of a lectin-like substance from rat liver.
D000818:Animals; D002241:Carbohydrates / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D007475:Ion Exchange Resins; D037102:Lectins / Q000302:isolation & purification*; D008099:Liver / Q000032:analysis; D051381:Rats
D002241:Carbohydrates; D007475:Ion Exchange Resins; D037102:Lectins