1H- and 13C-NMR spectroscopic study of glucose metabolism in eggs of Angiostrongylus cantonensis during their development.
1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy was used to study aerobic glucose metabolism in eggs of Angiostrongylus cantonensis in an NCTC-109 medium supplemented with fetal calf serum. Without any pretreatment of the spent medium, we were able to identify and quantitate, by NMR, the end-products of glucose metabolism in eggs after cultivation for 2, 4, and 8 days. We demonstrated that A. cantonensis eggs took up glucose rapidly; among the major end products were found lactic acid, acetic acid and alanine. The eggs are parasitic in a sense that the energy metabolism in them is dependent mainly upon the energy source present in outer medium.
D000801:Angiostrongylus; D000818:Animals; D050260:Carbohydrate Metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008681:Metastrongyloidea; D010063:Ovum / Q000378:metabolism*
D005947:Glucose