Circulating intact parathyroid hormone measured by a two-site immunochemiluminometric assay.
A direct immunoassay for circulating intact human PTH (hPTH) is described. The method relies on the formation of an immune complex of labeled antiamino-terminal PTH antibody, intact hPTH, and solid phase antimidregion PTH antibody. A chemiluminescent aryl acridinium ester is used as label. Serum samples (100 microL) are incubated with labeled antibody, and subsequently the bound fraction is separated by the addition of solid phase antibody. The bound luminescence is quantitated in an automatic luminometer. Luminescence intensity is directly proportional to the amount of intact PTH present in the sample. Only intact PTH was found to react in this system; there was no significant interference from PTH fragments. The assay detection limit of 0.8 pmol/L hPTH-(1-84) allowed detection of intact PTH in the serum of all normal subjects tested. A clear distinction was found between hypercalcemic individuals subsequently proven to have primary hyperparathyroidism and those with malignancies. The assay offers several advantages over previously described PTH immunoassays with regard to specificity, rapidity, and reagent stability. It, thus, provides a valuable means of investigating parathyroid physiology and clinical disorders of extracellular calcium metabolism.
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006934:Hypercalcemia / Q000097:blood; D006961:Hyperparathyroidism / Q000097:blood; D007118:Immunoassay / Q000379:methods*; D007120:Immunochemistry; D007676:Kidney Failure, Chronic / Q000097:blood; D008163:Luminescent Measurements; D008875:Middle Aged; D010280:Parathyroid Glands / Q000502:physiology; D010281:Parathyroid Hormone / Q000097:blood*; D012016:Reference Values
D010281:Parathyroid Hormone