title: Antibody to coliform antigens in urine samples from patients with symptoms of urinary tract infection.
abstract: An enzyme linked immunoadsorbent assay (ELISA) was used to detect IgG, IgM, and IgA antibody in urine to a crude antigenic mixture prepared from six arbitrarily chosen strains of common coliform urinary pathogens. In a group of patients with symptoms of urinary tract infection 24 of 80 (30%) urine samples were positive on culture by conventional methods, 58 of 80 (72.5%) were positive for polymorphs on microscopy, and 72 of 80 (90%) were positive for antibody by ELISA. Secretory component was detected in 28 of 80 (35%) samples, and this is consistent with local antibody production. The results show that antibody can be detected in a wide range of urine samples using a simple antigen mixture, and this may form the basis of a useful test to diagnose urinary tract infection.
mesh_terms: D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D004755:Enterobacteriaceae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000652:urine; D007074:Immunoglobulin G / Q000652:urine; D007075:Immunoglobulin M / Q000652:urine; D007136:Immunoglobulins / Q000652:urine*; D008297:Male; D014552:Urinary Tract Infections / Q000276:immunology* / Q000652:urine
chemical_list: D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins