title: Developmental expression of crystallin genes: in situ hybridization reveals a differential localization of specific mRNAs.
abstract: The time and place of the accumulation of alpha A-, beta B1- and gamma-crystallin RNA in the developing rat lens have been studied by in situ hybridization. alpha A- and gamma-crystallin RNA were first detected in the lens vesicle, while beta B1-crystallin RNA could be seen only after elongation of the primary fiber cells. Both beta B1- and gamma-crystallin RNA were confined to the fiber cells of fetal lenses, while alpha A-crystallin mRNA could also be detected in the epithelial cells. A quantification of the hybridization pattern obtained in the differentiation zone of the newborn rat lens showed that alpha A-crystallin RNA is concentrated in the cortical zone. alpha B-crystallin mRNA has the same distribution pattern. beta B1-crystallin RNA was relatively poorly detectable by in situ hybridization in both fetal and newborn rat lenses. The grain densities obtained with this probe increased from the periphery of the lens toward the interior, indicating that beta B1-crystallin RNA accumulated during differentiation of the secondary fiber cells. A similar accumulation pattern was obtained for gamma-crystallin mRNA, but, unexpectedly, this RNA could also be detected in the elongating epithelial cells. Our results show that gamma-crystallin RNA starts to accumulate as soon as visible elongation of epithelial cells occurs, during differentiation of the primary as well as the secondary fiber cells.
mesh_terms: D000818:Animals; D003459:Crystallins / Q000235:genetics*; D005314:Embryonic and Fetal Development; D005786:Gene Expression Regulation; D005796:Genes; D007908:Lens, Crystalline / Q000166:cytology / Q000196:embryology*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats; D014158:Transcription, Genetic
chemical_list: D003459:Crystallins