Application of the stoichiometric displacement model of retention to anion-exchange chromatography of nucleic acids.
The stoichiometric displacement model has been refined in its application to anion-exchange chromatography. The revised stoichiometric displacement model has been shown to be valid for anion-exchange chromatography with respect to all particulars of the model tested. It has been shown that the use of displacing agent activity is not a necessary condition for valid application of the stoichiometric displacement model to anion-exchange chromatography. While the cation of the displacing salt can influence anion-exchange chromatography, the data indicate that the displacing anion is of primary importance. It has been shown that solutes with three-dimensional structure have Zn value to solute charge ratios less than unity, and that the stoichiometric displacement model may be useful as a probe of solute three-dimensional structure.
D000225:Adenine / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007202:Indicators and Reagents; D008956:Models, Chemical; D009696:Nucleic Acids / Q000302:isolation & purification*; D009711:Nucleotides / Q000302:isolation & purification; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis; D013936:Thymidine / Q000032:analysis
D007202:Indicators and Reagents; D009696:Nucleic Acids; D009711:Nucleotides; D012346:RNA, Transfer, Amino Acyl; D000225:Adenine; D013936:Thymidine