Antigenic characterization of excretory-secretory products of Setaria cervi.
Excretory-secretory products (ES) of Setaria cervi, a bovine filarial parasite, were prepared by in vitro maintenance of the worms in a protein free defined medium at 37 degrees C. RPMI-1640 medium proved to be the best as the worms remained motile in it for the maximum period of time without change of medium, and approximately 12-15 mg protein per 100 adult worms could be obtained. Crossed immunoelectrophoretic analysis of ES products revealed 11-15 antigens and most of them were common to somatic antigenic preparations from both adult worms and microfilariae. The ES products were also found to contain 3-4 host proteins, including serum albumin.
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D003470:Culture Media; D005260:Female; D005370:Filarioidea / Q000276:immunology*; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D011817:Rabbits; D012719:Setariasis / Q000469:parasitology
D000947:Antigens, Helminth; D003470:Culture Media