Isolation of a sequence which maps close to the human sex determining gene.
A sequence mapping close to the human sex determining gene (TDF) has been isolated from a lambda library constructed with DNA derived from a chromosome transfectant hybrid cell line. This sequence is shown to be present in the DNA of X-Y interchange males at a very high frequency and, based on these studies, it is categorised with the sequence defined by the probe, GMGY3, as the closest known Y chromosome derived marker to TDF. In contrast to GMGY3, however, this locus shares no homology with any other human chromosome. Southern blot analysis also reveals specific hybridization to the Y chromosome of other primates. It therefore defines, for the first time, a conserved and Y chromosome unique locus that is near to TDF.
D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D006801:Humans; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis; D014162:Transfection; D014998:Y Chromosome