Ontogenesis of muscarinic receptors in cultured rat hippocampal cells.
The ontogeny of muscarinic cholinergic receptors was studied in primary cultures of dissociated rat hippocampal cells. The specific [3H]quinuclidinyl benzilate [( 3H]QNB) to intact cells was detected after two days in culture. Scatchard analysis revealed only a unique binding site at all of the days tested, with a very high affinity (0.205-0.253 nM) for the substrate. Specific binding kept increasing up to a level of 2035 fmol/mg protein after 15 days in culture. Protein content by itself also increased over time, reaching the highest level at 15 days (286 micrograms protein/35 mm plate). The cultured hippocampus can thus serve as a model system for the study of the development of muscarinic receptors in vitro.
D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000254:growth & development* / Q000378:metabolism; D007700:Kinetics; D011506:Proteins / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*
D011506:Proteins; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate