A possible mechanism of increased sodium influx in red cells with abnormal membrane lipid levels induced by phospholipase A2.
Membrane transport--sodium (Na+) influx and calcium (Ca2+) uptake--was examined in human mature red cells treated with phospholipase A2 (PLase A2) from snake venom. PLase A2-induced conversion of phosphatidyl choline (PC) to lysophosphatidyl choline (L-PC) was associated with a marked increase in Na+ influx and Ca2+ uptake. After L-PC was removed from the cell membrane of the PLase A2-treated red cells in the presence of albumin, an additional increase in Ca2+ transport was observed. These results indicate that membrane lipid abnormalities, such as increased L-PC and/or a loss of total lipids, appear to induce increased membrane transport.
D000255:Adenosine Triphosphate / Q000097:blood; D001692:Biological Transport; D002118:Calcium / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000097:blood; D008563:Membrane Lipids / Q000097:blood*; D010713:Phosphatidylcholines / Q000097:blood; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2; D011188:Potassium / Q000097:blood; D012709:Serum Albumin / Q000502:physiology; D012964:Sodium / Q000097:blood*
D008244:Lysophosphatidylcholines; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D012709:Serum Albumin; D000255:Adenosine Triphosphate; D012964:Sodium; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D011188:Potassium; D002118:Calcium