Semiquantitative evaluation of histochemically detectable calcium binding in mammalian brain slices.
A method is described for the electron histochemical demonstration of Ca in the central nervous system, based on fixation on Ca-containing paraformaldehyde solution and the subsequent complexing of Ca by ammonium oxalate. The method resulted in highly electron-dense deposits, with good ultrastructural preservation. The Ca content of the deposits was proved by physico-chemical analysis. The high electron density permitted the counting of deposits and thereby an estimation of their numerical density, via planimetry of electron micrographs. Since pre- and postsynaptic localizations could be distinguished on the basis of ultrastructure, this procedure is regarded as a unique semiquantitative method for estimation of the tissue Ca binding of mammalian brain slices.
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism*; D006168:Guinea Pigs; D006651:Histocytochemistry / Q000379:methods*; D066298:In Vitro Techniques; D008854:Microscopy, Electron
D002118:Calcium