title: Megakaryocyte fragments and the microtubule coil.
abstract: We have examined megakaryocyte process fragments that migrate out of bone marrow explants after a short period of incubation and assume a beaded form, consisting of 2 or more putative platelets. The fragmentation appears to occur in vivo and supports the proposal that platelet liberation does not always occur in a sequential manner from the distal ends of megakaryocyte processes. Transmission electron microscopy revealed that microtubules were generally oriented longitudinally in the process fragments. Rarely, a microtubule coil was found in a terminally located putative platelet. The observations favour the view that the marginal coil of microtubules, which is a characteristic of circulating platelets, does not usually form until after platelets have been liberated.
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D001854:Bone Marrow Cells; D007700:Kinetics; D008297:Male; D008533:Megakaryocytes / Q000166:cytology* / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000648:ultrastructure*; D009924:Organ Culture Techniques