Radio gas chromatography for evaluation of sub-cellular hormone synthesis in the androgen insensitivity syndrome.
This paper gives a description of a radio gas-liquid chromatographic method for the evaluation of androgen hormone synthesis patterns in the testicular tissue of a patient suffering from the androgen insensitivity syndrome (AIS). A modified dual-column gas chromatography system, equipped with column switching facilities and a radioactivity monitor run parallel to a flame ionisation detector, enables the monitoring of radioactive intermediates of testosterone anabolism and catabolism, generated from a labelled precursor. Tissue preparations were incubated with tritiated pregnenolone for 45 min at 37 degrees C. Steroid hormones were stripped from the aqueous phase by solvent extraction and analysed by gas chromatography as methoxitrimethylsilyl (MO-TMS) derivatives on a 15 m X 0.32 mm I.D. fused-silica capillary column coated with DB-5. The results reveal abnormal enzyme kinetics due to accelerated precursor utilisation. The findings reflect the pathophysiology of AIS at the sub-cellular level of the androgen hormone target organ.
D000293:Adolescent; D000728:Androgens / Q000172:deficiency*; D002849:Chromatography, Gas; D006728:Hormones / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013577:Syndrome; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000032:analysis / Q000096:biosynthesis
D000728:Androgens; D006728:Hormones; D013739:Testosterone