Characterization of a silent gene for human pancreatic elastase I: structure of the 5'-flanking region.
A cDNA for porcine pancreatic elastase I has been cloned and used as a probe for blot hybridization analysis. Southern blot analysis of total DNA demonstrated that the elastase I gene is conserved among the porcine, rat, calf, and human genomes. Northern blot analysis, however, indicated that the elastase I gene is not expressed in the human adult pancreas, even though abundant expression is observed in the rat and porcine pancreas. Sequence analysis of the cloned human elastase I gene revealed that the proximal 5'-flanking region from -1 to -200 shows 78% identity with that of the actively transcribed rat elastase I gene, and contains a consensus TATA box and a putative tissue specific enhancer sequence. The transcriptional activity of the human elastase I gene appears to be suppressed in the human adult pancreas.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D006801:Humans; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000235:genetics*; D051381:Rats; D013045:Species Specificity; D013552:Swine; D014158:Transcription, Genetic
D004247:DNA; D010196:Pancreatic Elastase