[Somatomedin C/insulin-like growth factor I and in vitro erythropoiesis].
The effect of human somatomedin C/insulin-like growth factor I(SM-C/IGF-1) and human growth hormone (hGH) on colony formation by erythroid precursor cells (CFU-E and BFU-E) from children's bone marrow or blood was studied by methylcellulose cloning assay. We found that physiological concentrations of IGF-1, but not of hGH, stimulated erythropoiesis in vitro in the presence of erythropoietin, as demonstrated by the increased activity of a cytosolic enzyme of the heme pathway (uroporphyrinogen I synthase). The results suggest that IGF-I could be involved in the regulation of erythroid differentiation.
D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002648:Child; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000378:metabolism; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D013002:Somatomedins / Q000502:physiology*
D013002:Somatomedins; D004921:Erythropoietin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D011163:Hydroxymethylbilane Synthase
Somatomédine C/"insulin-like growth factor" I (SMC/IGF-I) et érythropoïèse in vitro.