Retrospective glycemic status of diabetic patients: glycosylation of blood proteins in diabetes and chronic renal failure.
In vivo and in vitro studies were carried out to evaluate the clinical application of glycosylated hemoglobin and plasma proteins in the diagnosis and management of diabetes mellitus. Glycosylated hemoglobin registered an almost 80% fall in diabetic patients following controlled glycemia for two months while glycosylated plasma protein level registered an 80% fall in the patients after fifteen days of blood glucose homeostasis. Human serum proteins were glycosylated in vitro and glycosylation was linearly proportional to the glucose concentration and incubation time. Polyacrylamide gel electrophoresis of glycosylated serum proteins revealed that albumin and transferrin are the major proteins that are significantly glycosylated. Glycosylated hemoglobin and plasma protein levels were also increased in chronic renal failure patients without any history of diabetes.
D001786:Blood Glucose / Q000378:metabolism*; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000378:metabolism*; D003920:Diabetes Mellitus / Q000097:blood*; D006442:Glycated Hemoglobin / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism*; D012189:Retrospective Studies
D001786:Blood Glucose; D001798:Blood Proteins; D006442:Glycated Hemoglobin; D006023:Glycoproteins; D006454:Hemoglobins