title: Relationship between the steroid and prolactin concentration in follicular fluid and the maturation and fertilization of human oocytes.
abstract: Seventy-eight follicles and their follicular fluid were aspirated from 46 women undergoing in vitro fertilization (IVF) procedures after stimulation of the ovaries with a low-dose human menopausal gonadotropin/human chorionic gonadotropin stimulation regimen. The concentrations of estradiol (E2), progesterone (P), testosterone (T), and prolactin (PRL) were measured in follicular fluid and related to the maturation of the oocyte-corona-cumulus complex (OCCC) and the fertilization of oocytes. Follicles containing mature oocytes had significantly higher follicular fluid E2 and P levels than follicles with intermediate and immature oocytes. A constant decrease in PRL and T values with advancing follicular maturation was observed. Similar results were obtained when the fertilizing ability of the oocytes was examined. The gradual decline in follicular fluid PRL and T levels during follicular development was connected with increasing E2 and P biosynthesis and therefore seems to be an important precondition for normal follicular and oocyte maturation.
mesh_terms: D000328:Adult; D001826:Body Fluids / Q000032:analysis*; D004958:Estradiol / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro; D012739:Gonadal Steroid Hormones / Q000032:analysis*; D006801:Humans; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*; D011374:Progesterone / Q000032:analysis; D011388:Prolactin / Q000032:analysis*; D011863:Radioimmunoassay; D013739:Testosterone / Q000032:analysis
chemical_list: D012739:Gonadal Steroid Hormones; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin