Assembly of pre-mRNA splicing complex is cap dependent.
To study the influence of the ubiquitous cap structure of nuclear pre-mRNAs on the assembly of a functional splicing complex, the in vitro splicing of a truncated human metallothionein pre-mRNA was examined in the presence of the cap analogue m7GTP. Significant inhibition of splicing was observed at a concentration as low as 5 microM m7GTP. Analysis of the splicing reaction on glycerol density gradients showed two complexes sedimenting at 45S and 22S. When the reaction was carried out in presence of m7GTP a marked decrease of the material sedimenting at 45S, representing the active splicing complex, was observed. When capped pre-mRNA was replaced by uncapped pre-mRNA, complex formation was significantly reduced. These data indicate that the cap structure plays an important yet unknown role in the assembly of spliceosomes.
D005091:Exons; D005796:Genes; D006801:Humans; D007438:Introns; D008668:Metallothionein / Q000235:genetics*; D009694:Nucleic Acid Precursors / Q000235:genetics*; D012315:RNA Caps; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
D009694:Nucleic Acid Precursors; D012315:RNA Caps; D012322:RNA Precursors; D012333:RNA, Messenger; D008668:Metallothionein