title: Retrospective analysis of the prognostic significance of DNA content and proliferative activity in large bowel carcinoma.
abstract: In the present study we have evaluated the prognostic significance of ploidy levels and proliferative activity in 279 cases of large bowel carcinomas which were included in a surgical prospective randomized trial. Ploidy levels and proliferative activity were determined on nuclei isolated from paraffin-embedded tissues of 279 colorectal carcinoma patients, with a mean follow-up of 51.9 months. Product limit survival analysis demonstrated a borderline significant association (P = 0.0689 by generalized Breslow; P = 0.0336 by generalized Savage) between ploidy and survival, with a 75th quantile survival of 49.8 months for patients with diploid tumors and 35.9 months for patients with aneuploid tumors. After stratification for staging, Dukes' C cases showed a statistically significant association between tumor ploidy and survival (P = 0.0224 by generalized Breslow, P = 0.0110 by generalized Savage). Product limit survival analysis for proliferative activity and survival showed a similar outcome with the strongest association in Dukes's C stage of disease (75th quantile survival of 38.9 months for low proliferative and 18.0 months for high proliferative tumors).
mesh_terms: D000782:Aneuploidy; D002455:Cell Division; D003110:Colonic Neoplasms / Q000235:genetics* / Q000601:surgery; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D009367:Neoplasm Staging; D011123:Polyploidy; D011379:Prognosis; D011446:Prospective Studies; D011897:Random Allocation; D012004:Rectal Neoplasms / Q000235:genetics* / Q000601:surgery; D012189:Retrospective Studies
chemical_list: D004247:DNA