title: An improved method for the colorimetric assay of lipase activity using an optically clear medium.
abstract: Lipase activity can be spectrophometrically measured in an optically clear medium using long chain fatty thioesters of 1-mercapto-2,3-propanediol or 2-mercaptoethanol as substrates. With hexamethylphosphoric triamide solutions of these thiosubstrates, the Michaëlis-Menten constants of lipase from Rhizopus arrhizus were determined. The effects of calcium chloride and of bovine serum albumin on the enzyme activity were established.
mesh_terms: D003124:Colorimetry / Q000379:methods; D007202:Indicators and Reagents; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D012233:Rhizopus / Q000201:enzymology
chemical_list: D007202:Indicators and Reagents; D008049:Lipase