Rabbit bone marrow glucose-6-phosphate dehydrogenase during erythroid cell development.
Studies were carried out on glucose-6-phosphate dehydrogenase (G6P-DH) during the differentiation of rabbit bone marrow erythroid cells. It was found that G6P-DH, although displaying a 7-fold activity decrease, did not change the relative amounts of its three dimeric forms. Using homogeneous enzyme preparations, we observed that from dividing to non-dividing erythroblasts the following properties remained constant: V max dependence on pH and temperature, Km for G6P dependence on pH, heat stability, 2-deoxy glucose-6-phosphate utilization, molecular weight, while the Km for NADP significantly increased in non-dividing erythroblasts. These results indicate that no shift towards the oxidized form of the enzyme and no substantial modifications of the protein take place during cell differentiation.
D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D002454:Cell Differentiation; D004900:Erythroblasts / Q000166:cytology / Q000201:enzymology*; D004920:Erythropoiesis; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006454:Hemoglobins / Q000032:analysis; D007700:Kinetics; D011817:Rabbits
D006454:Hemoglobins; D005954:Glucosephosphate Dehydrogenase