Developmental changes in the lateral diffusion of Leydig cell membranes measured by the FRAP method.
A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility of membrane lipids and proteins were discovered.
D000375:Aging; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D004058:Diffusion; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000502:physiology*; D008297:Male; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013737:Testis / Q000254:growth & development*
D005456:Fluorescent Dyes; D008563:Membrane Lipids; D008565:Membrane Proteins