In vitro drug screening in isolated male Onchocerca gibsoni using motility suppression.
A primary in vitro screen was developed to screen for drug activity against isolated Onchocerca gibsoni. The assay estimates variation in motility through the use of a motility meter. Of the seven compounds tested in the screen; ivermectin, CGP 6140, CGP20376, Mel W and furapyrimidone gave MI50 concentrations (the concentration at which the motility was reduced to 50% of the control value at 72 hours) below 10(-4) M, whereas suramin gave variable results depending on the varying susceptibility of individual worms and levamisole at 10(-4) M had no significant effect on the worms. The effects of these drugs were not reversible as removal of the worms into drug-free medium caused no increase in motility. Thus the reduction in motility is regarded as indicating significant metabolic damage. The results compared favourably with reported in vivo tertiary screens for activity against Onchocerca species. This is a quantitative, inexpensive and reproducible method for assessing the effectiveness of drugs against Onchocerca and could be included into the primary screens for activity against filarial worms.
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001152:Arsenicals / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D005369:Filaricides / Q000494:pharmacology*; D007559:Ivermectin / Q000494:pharmacology; D007978:Levamisole / Q000494:pharmacology; D008297:Male; D009068:Movement / Q000187:drug effects; D009581:Nitrofurans / Q000494:pharmacology; D009854:Onchocerca / Q000187:drug effects* / Q000502:physiology; D010879:Piperazines / Q000494:pharmacology; D013498:Suramin / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D000871:Anthelmintics; D001152:Arsenicals; D005369:Filaricides; D009581:Nitrofurans; D010879:Piperazines; D013844:Thiazoles; D007978:Levamisole; C015980:melarsonic acid; D013498:Suramin; D007559:Ivermectin; C032572:furapyrimidone; C051745:CGP 20376; C051744:amocarzine