Inhibition of insect juvenile hormone synthesis by phorbol 12-myristate 13-acetate.
The synthesis of insect juvenile hormone III (JH III) by isolated corpora allata of the cockroach Diploptera punctata incubated in vitro is inhibited by phorbol 12-myristate 13-acetate (PMA), phorbol 12,13-dibutyrate and 1-oleyl-2-acetylglycerol. 4 alpha-Phorbol 12,13-didecanoate and diolein are inactive. The inhibitory effect of phorbol 12-myristate 13-acetate is fully reversed by 2E,6E-farnesol or by 2E,6E-farnesoic acid. It is highest in corpora allata that are past their peak in secretory activity or that have been inhibited by injections of 20-hydroxyecdysone. This effect of phorbol esters implicates protein kinase C in the regulation of insect corpus allatum activity.
D000818:Animals; D003058:Cockroaches; D004441:Ecdysterone / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007605:Juvenile Hormones / Q000096:biosynthesis*; D009865:Oocytes / Q000201:enzymology; D011493:Protein Kinase C / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D007605:Juvenile Hormones; D004441:Ecdysterone; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate