Enhancement of fluorescence of pyrene-containing lipids by polar media, detergents and phospholipids.
The fluorescence intensities of a medium-chain fatty acid and of several amphiphilic lipids, each containing pyrene in covalent linkage, were enhanced considerably by: 1) Dissolving in mixtures of a polar solvent (e.g. methanol, ethanol, tetrahydrofuran or dimethylsulfoxide) and water; for each individual compound, a certain ratio of solvent to water provided maximal fluorescence intensity. 2) Incorporating into micelles of reduced Triton X-100; an excess of detergent was used so that, statistically, only one molecule of lipid resided in one micelle of the Triton X-100. 3) Incorporating into liposomes of egg phosphatidylcholine; maximal fluorescence was observed using a large excess of phosphatidylcholine. When related to the fluorescence intensities in chloroform/methanol (2:1, by vol.) or water, the enhancement of fluorescence in the above three systems was about 2-6-fold or up to 60-fold, respectively.
D002518:Ceramides / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005453:Fluorescence; D007850:Lauric Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008081:Liposomes / Q000378:metabolism; D000432:Methanol / Q000494:pharmacology; D017830:Octoxynol; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011721:Pyrenes / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013109:Sphingomyelins / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology*
D002518:Ceramides; D003902:Detergents; D005227:Fatty Acids; D007850:Lauric Acids; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D011721:Pyrenes; D012997:Solvents; D013109:Sphingomyelins; D013501:Surface-Active Agents; C030358:lauric acid; D011092:Polyethylene Glycols; D017830:Octoxynol; D000432:Methanol