Opal suppressor phosphoseryl-tRNA is not a substrate of phosphoserine aminotransferase.
A proposal of the role of animal opal suppressor phosphoseryl (Ps)-tRNA is that Ps-tRNA plays a role as an intermediate in the metabolic pathway from 3-phosphoglycerate to glycine. The labeled [32P]phospho[3H]seryl-tRNA was prepared and used as a substrate in the reaction of bovine brain Ps aminotransferase (EC 2.6.1.52) in the presence of alpha-ketoglutarate. By analysis of the reaction product, no amount of 3-phosphohydroxypyruvate was found, even though phosphohydroxypyruvate was noted in the control experiment by use of Ps and alpha-ketoglutarate. These results showed that Ps-tRNA was not a substrate of Ps aminotransferase.
D000818:Animals; D007700:Kinetics; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D013379:Substrate Specificity; D013489:Suppression, Genetic; D000637:Transaminases / Q000378:metabolism*
D012346:RNA, Transfer, Amino Acyl; C043354:tRNA, O(beta)-phosphoserine-; D000637:Transaminases; C022393:phosphoserine aminotransferase