Binding of non-histone chromosomal protein HMG1 to histone H3 in nucleosomes detected by photochemical cross-linking.
The interaction of non-histone chromosomal protein HMG1 with core histones in nucleosomes was studied via reconstitution and photochemical cross-linking. The results obtained indicated that photoaffinity-labeled HMG1 interacted in nucleosomes with histone H3. Similar experiments with peptides derived from HMG1 by V8 protease digestion allowed to identify N-terminal domain of HMG1 (peptide V3) as a binding region for histone H3 in nucleosomes.
D000818:Animals; D002645:Chickens; D003432:Cross-Linking Reagents; D004912:Erythrocytes; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D009707:Nucleosomes / Q000648:ultrastructure*; D011485:Protein Binding
D003432:Cross-Linking Reagents; D006609:High Mobility Group Proteins; D006657:Histones; D009707:Nucleosomes