Computer aided prediction and evaluation of the tertiary structure for rat elastase II.
Predictive methods have been extended to generate a proposed tertiary structure of rat elastase II on the basis of primary amino acid sequence and structural homologies within the family of mammalian serine proteinases. Force field refinement calculations were used to relax the structure. Structurally conserved molecules of solvation were introduced and the structure was again refined by means of force-field calculations. The resulting structure suggests probable substrate cleavage preferences. An independent statistical analysis of the crystallographically refined structures of serine proteinases (0.01-0.13 A, RMS) shows a close similarity to the final predicted model of rat pancreatic elastase II (0.03-0.14 A, RMS).
D000595:Amino Acid Sequence; D000818:Animals; D002918:Chymotrypsin; D003201:Computers; D006860:Hydrogen Bonding; D008958:Models, Molecular; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase; D011487:Protein Conformation; D051381:Rats; D013379:Substrate Specificity; D013552:Swine; D014867:Water
D014867:Water; D002918:Chymotrypsin; D010196:Pancreatic Elastase