title: Natural cytotoxic cells from rat liver and spleen kill human glioma cells.
abstract: Natural cytotoxic cells from rat spleen and rat liver, (isolated using the collagenase method) were found to be cytotoxic against different lines of human gliomas: T406, T508, T705, HeRo, HeRoCl 1, HeRoCl 8, and HeRo-SV 7/114. After 18 h the lytic units ranged from 75 to 251 in the liver and from 5 to 24 in the spleen. Analyzing the ratio of lysis at 4 h/18 h, it may be concluded that this natural killing is predominantly macrophage (Kupffer cell)-dependent. Lysis by Kupffer cells cannot be increased by transforming glioma cells with SV40. Experiments with SV40-transformed mouse fibroblasts (3T3) and virus-transformed human cell lines (SV80) suggested a "SV40" receptor on Kupffer cells. Thus Kupffer cells have receptors for glioma cells and SV40-dependent membrane structures.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005910:Glioma / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051381:Rats; D013154:Spleen / Q000276:immunology*