Antisera raised against the drug imipramine.
Antisera against 2-aminoimipramine covalently coupled to albumin have been raised in two rabbits. Both antisera bind imipramine and related tricyclic compounds as if to a single class of sites with high affinity and high titres. Displacement/inhibition assays showed that the affinities of various tricyclic compounds for the antisera showed a good correlation with the affinities of these drugs for the tricyclic antidepressant inhibitory sites on plasma-membrane 5-hydroxytryptamine carriers of human platelets and rat brain cortex. 5-Hydroxytryptamine and 5-hydroxytryptamine-uptake-selective drugs did not inhibit [3H]imipramine binding to antisera. The anti-imipramine antibodies were purified using imipramine-Sepharose affinity chromatography and were shown to be IgG class by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and protein A-Sepharose precipitation.
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D000915:Antibody Affinity; D001792:Blood Platelets / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007099:Imipramine / Q000276:immunology* / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D008970:Molecular Weight; D011817:Rabbits / Q000276:immunology; D051381:Rats
D000906:Antibodies; D007106:Immune Sera; D007074:Immunoglobulin G; D007099:Imipramine