[Autoradiography studies of the effect of oxaceprol on the metabolism of joint cartilage in vitro and in vivo].
Using the radiolabelled precursors (3H-Glucosamine, 3H-proline) and a new procedure of quantitative microautoradiography we investigated the influence of N-acetyl-L-hydroxyproline (Oxaceprol) on anabolic processes in joint cartilage tissue of hens. After in vitro incubation, Oxaceprol stimulated the uptake of 3H-glucosamine and 3H-proline in chondrocytes and enhanced the incorporation of 3H-proline into the macromolecular structures of the cartilage matrix. Also, after in vivo intra-articular injection of Oxaceprol into the knee joints we could demonstrate a significant increase of intracellular glucosamine uptake. Because in our micro-autoradiographic detection method low molecular substances are excluded, the higher rate of precursor incorporation into cells and matrix can be interpreted as a stimulation of proteoglycan and collagen synthesis.
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001345:Autoradiography; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002645:Chickens; D004734:Energy Metabolism / Q000187:drug effects*; D005944:Glucosamine / Q000378:metabolism; D006801:Humans; D006909:Hydroxyproline / Q000031:analogs & derivatives* / Q000494:pharmacology; D011392:Proline / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011509:Proteoglycans; D011392:Proline; D005944:Glucosamine; C012393:oxaceprol; D006909:Hydroxyproline
Autoradiographische Untersuchungen über den Einfluss von Oxaceprol auf den Stoffwechsel des Gelenkknorpels in vitro und in vivo.