Detection of trophoblast-like cells in maternal blood using specific monoclonal antibodies.
Mouse monoclonal antibodies reacting against membrane antigens expressed on syncytiotrophoblast (McAb H315), villous cytotrophoblast (McAb 18B/A5) and placental-type alkaline phosphatase-producing cells (McAb H317) were used in conjunction with flow cytometry to detect placental cells in the maternal circulation. H315 and H317-positive cells were found in uterine veins and peripheral blood of pregnant women, while cells reacting with McAb 18B/A5 were either absent or present in very low numbers.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005260:Female; D006801:Humans; D011247:Pregnancy / Q000097:blood* / Q000276:immunology; D014327:Trophoblasts / Q000166:cytology* / Q000276:immunology; D014599:Uterus / Q000098:blood supply
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface