Comparison of the immunocytochemical assay (ER-ICA) and the biochemical assay for estrogen receptor in human breast cancer cell lines.
Estrogen receptor content of 8 cell lines from non-malignant human breast tissues was determined by an immunocytochemical assay using the Abbott ER-ICA monoclonal kit. The results were in accordance with those obtained by the conventional radiochemical (DCC) assay. Primary cultures of breast tissues are suggested as an important field for in situ application of the ER-ICA.
D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000032:analysis*; D002460:Cell Line; D006801:Humans; D007118:Immunoassay; D011933:Reagent Kits, Diagnostic; D011960:Receptors, Estrogen / Q000032:analysis*
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D011960:Receptors, Estrogen