Comparison among lipid constituents in native LDL, ultra-water-soluble LDL, and vessel wall, and their significance in arteriosclerosis.
The lipid constituents in native low-density lipoproteins (LDL), ultra-water-soluble LDL (UWS-LDL), and aortic intimal tissues were compared. These lipoproteins were obtained from healthy persons and patients with atherosclerotic diseases. Also, aortic intimas were separated from arterial walls obtained within 5 hr after the donor's death. (1) From the native LDL, cholesterol esters (CE), triglycerides (TG), small amounts of free fatty cid (FFA), free cholesterol (CF), and phospholipids (PL) were demonstrable by iodine vapor on TLC, but from UWS-LDL the above lipids plus a new lipid (spot X) were observed between TG and FFA on TLC. And also, an unknown spot with the same Rf value as spot X was recognized on TLC of lipid extract from the atherosclerotic lesion, but not from the normal intima. (2) The production of spot X in UWS-LDL is probably related to the oxidation of lipids in native LDL. Also, the spot X in UWS-LDL and the spot X in the atherosclerotic lesions are probably related to the oxidation of CE in these lipids. (3) The existence of UWS-LDL is important to the initiation and probably the progression of atherosclerosis.
D001011:Aorta / Q000032:analysis; D001158:Arteries / Q000032:analysis*; D001161:Arteriosclerosis / Q000378:metabolism*; D002784:Cholesterol / Q000032:analysis; D002855:Chromatography, Thin Layer; D003956:Dialysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis* / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D010100:Oxygen; D010743:Phospholipids / Q000032:analysis; D012016:Reference Values; D012995:Solubility; D014280:Triglycerides / Q000032:analysis
D005230:Fatty Acids, Nonesterified; D008055:Lipids; D008077:Lipoproteins, LDL; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol; D010100:Oxygen