Inhibitory effect of carp muscle trypsin inhibitor on mammalian pancreatic proteinases.
Carp muscle trypsin inhibitor showed an inhibitory effect on bovine trypsin, bovine alpha-chymotrypsin and porcine elastase in a non-competitive, competitive and competitive manner, respectively. The inhibitor formed a stable complex with the above proteinases which was not dissociated in the presence of 2-mercaptoethanol and SDS. The true target proteinase for carp muscle trypsin inhibitor, as yet unknown, seems to be an alpha-chymotrypsin- or elastase-like enzyme rather than trypsin, judging from the manner of inhibition.
D000818:Animals; D002347:Carps; D002417:Cattle; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D007700:Kinetics; D009132:Muscles / Q000502:physiology*; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D013552:Swine; D014361:Trypsin Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*
D014361:Trypsin Inhibitors; D002918:Chymotrypsin; D010196:Pancreatic Elastase