Sodium binding to and protonation of ATP: a multinuclear magnetic double resonance study at 8.46 tesla.
We show that the interaction of ATP with Na+ and H+, whether binding or dissociation, gives rise to exchange broadened 31P-NMR spectra at 8.4 T, pH 6.7 and 310 K. We interpret the effect as being due to a two-step conversion between two NMR-differentiated ATP pools. A quantitative analysis yields all involved equilibrium constants and some of the dynamic parameters. Our results help to understand previous studies of magnesium binding to ATP and the appearance of high-field in vivo 31P-NMR spectra.
D000255:Adenosine Triphosphate; D007700:Kinetics; D008274:Magnesium; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D012964:Sodium; D012965:Sodium Chloride
D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D012964:Sodium; D008274:Magnesium