A new method to express embryotoxic data obtained in vitro on whole murine embryos.
This report proposes to express the effect of drugs in the whole embryo culture system by a new method using an intrinsic reference. Percentages of malformed embryos or other defects in development, expressed as percentages of controls, are plotted against concentrations of the drug expressed as percentages of the concentration inducing 50% embryolethality (ELC50). It is suggested that the slope generated by this method is directly related to the intensity of the in vitro teratogenic potential of the agent and allows an estimation of the specific interference with developmental processes. The method has been applied to data obtained from the literature and pertaining to 22 drugs. The slope generated by these drugs varied widely. The effect of these drugs could be meaningfully compared in spite of the wide range of ELC50 displayed by the drugs. In addition, results obtained for two drugs in different laboratories using different methods and species were in fair agreement when they were compared by using the proposed method. Finally, it is suggested that the method provides an improved means to inquire if there is any relevance of in vitro data to teratological results obtained in vivo.
D000818:Animals; D046508:Culture Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013723:Teratogens
D011506:Proteins; D013723:Teratogens