Isolation of complement protein D from urine of patients with Fanconi's syndrome.
Complement protein D is the least abundant of all complement proteins and, thus, one of the most difficult to purify. We report a new method for obtaining pure D from urine of patients with Fanconi's syndrome. The method is simple and allows the purification of milligram amounts of D within a few days. It involves three chromatographic steps using Bio-Rex 70, hydroxylapatite HPLC, and reverse-phase HPLC. Protein D purified by this method is suitable for both functional and structural studies.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D003166:Complement Activating Enzymes / Q000652:urine*; D011416:Complement Factor D / Q000302:isolation & purification / Q000652:urine*; D004589:Electrophoresis, Disc; D005198:Fanconi Syndrome / Q000652:urine*; D006801:Humans
D003166:Complement Activating Enzymes; C493488:CFD protein, human; D011416:Complement Factor D