Parinaric acid as a sensitive fluorescent probe for the determination of lipid peroxidation.
The decrease in fluorescence of conjugated polyenic acyl chains is used as a sensitive assay for lipid peroxidation. The fatty acid cis-trans-trans-cis-9,11,13, 15-octadecatetraenoic acid (cis-parinaric acid) is introduced into liposomal membranes as free fatty acid or, by using the PC specific transfer protein from bovine liver, as 1-palmitoyl-2-cis-parinaroyl-sn-glycero-3-phosphocholine. The peroxidation process as monitored by the decrease in fluorescence intensity is compared with other peroxidation assay systems. Applications of the new assay system are discussed.
D000727:Androgen-Binding Protein; D002352:Carrier Proteins / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D049294:Phospholipid Transfer Proteins; D013050:Spectrometry, Fluorescence
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D008054:Lipid Peroxides; D008081:Liposomes; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D049294:Phospholipid Transfer Proteins; C030048:2-parinarolyllecithin; D003300:Copper; D006861:Hydrogen Peroxide; C013641:parinaric acid