A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles.
D000818:Animals; D005230:Fatty Acids, Nonesterified / Q000097:blood; D007202:Indicators and Reagents; D008264:Macrophages / Q000032:analysis; D051379:Mice; D009532:Nickel; D011868:Radioisotopes; D014280:Triglycerides / Q000097:blood*; D014304:Triolein / Q000032:analysis
D005230:Fatty Acids, Nonesterified; D007202:Indicators and Reagents; D011868:Radioisotopes; D014280:Triglycerides; D014304:Triolein; D009532:Nickel