Sperm-egg penetration of human spermatozoa treated with various rabbit antisera to human sperm antigens.
The effect of rabbit antisera to human whole spermatozoa, to human lactate dehydrogenase (LDH-X) and to human protamine 1 (HP1) and protamine 2 (HP2), was tested in the hamster zona-free egg penetration assay with human spermatozoa. The human spermatozoa were incubated with TEST yolk buffer washed and incubated with the antisera before penetration assay. Only antiserum to whole spermatozoa could inhibit the penetration capacity of treated human spermatozoa. The significance of the results as well as the advantage of the method used by us are discussed.
D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D006224:Cricetinae; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007770:L-Lactate Dehydrogenase / Q000276:immunology; D008297:Male; D011479:Protamines / Q000276:immunology; D011817:Rabbits; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000276:immunology*
D001324:Autoantigens; D007106:Immune Sera; D011479:Protamines; D007770:L-Lactate Dehydrogenase