A personal computer program using a 4-component logit for the evaluation of ELISA calibration curves with unknown asymptotic values.
A BASIC program is described for the calculation of ELISA calibration curves using a 4-component logit for the linearisation of the sigmoidal shape. The difficulty of estimating the four logit parameters without knowledge of upper and lower asymptotic values is overcome by statistical analysis of the experimental data. The program is run on an IBM-PC and the curves are plotted by a HP7475A plotter.
D001699:Biometry; D003201:Computers; D004797:Enzyme-Linked Immunosorbent Assay; D008838:Microcomputers; D012984:Software