title: Expression by human fetal organs of organ-specific cancer neoantigens as measured by leukocyte adherence inhibition.
abstract: Human cancers express organ-specific cancer neoantigens (OSN) as determined by in vitro leukocyte responses to extracts of cancers by the tumor host. In this study, we determined whether the OSNs were normal developmental proteins that were expressed by fetal organs and re-expressed with oncogenesis. Fetal extracts, principally of lung and colon but also of liver and kidney, were tested for their ability to induce leukocyte adherence inhibition (LAI) as compared to extracts from adult tissues of the same organ. Leukocytes from lung cancer patients showed positive LAI responses to 13- and 19-week fetal lung tissue. Likewise, leukocytes from colon cancer patients showed positive LAI responses to 14- and 19-week fetal colon tissue, whereas leukocytes from control subjects did not. Neither group responded positively to 21-week fetal organs. Criss-cross experiments showed that the fetal antigen was organ specific. Multiparous pregnant women showed positive LAI responses to cancer extracts but not to extracts from normal tissues of the same organ. The pattern of the LAI response was bell-shaped. Positive LAI responses to lung and breast cancer were detected at 4 to 7 months gestation and peaked at 5 months. To the fetal colon, LAI positive responses were detected at 5 to 8 months gestation, with the peak response at 6 months. The results indicate that OSN of cancers are also expressed by fetal organs and sufficient antigen is shed by fetal organs to sensitize pregnant women. Older fetal organs (21 weeks) and adult organs do not express an immunogenic or antigenic OSN.
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D003106:Colon / Q000196:embryology / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology; D005260:Female; D005333:Fetus / Q000032:analysis*; D006801:Humans; D007111:Immunity, Cellular; D007668:Kidney / Q000196:embryology / Q000276:immunology; D007680:Kidney Neoplasms / Q000276:immunology; D007957:Leukocyte Adherence Inhibition Test; D008168:Lung / Q000196:embryology / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology; D009369:Neoplasms / Q000276:immunology*; D010298:Parity; D011247:Pregnancy
chemical_list: D000951:Antigens, Neoplasm