Use of Vero cells for the isolation and propagation of malignant catarrhal fever virus.
Vero cells were compared with primary bovine thyroid (BTh) cultures for the isolation of malignant catarrhal fever virus from infected blood and tissues. Comparative titrations showed Vero cells detected only two-fold less infectivity in rabbit spleen suspensions than BTh cells. Twenty three of 32 bovine buffy coat cell preparations which were positive on BTh cells were also positive on Vero cells. The cytopathic effects (CPE) of virus isolates in Vero cells consisted of syncytia and refractile cytomegalic cells which were as easy to recognise and developed as rapidly as CPE in BTh cells. Two laboratory strains of malignant catarrhal fever virus were readily adapted to and maintained by passage in Vero cells.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006564:Herpesviridae / Q000254:growth & development / Q000302:isolation & purification*; D008304:Malignant Catarrh / Q000382:microbiology; D011817:Rabbits; D014709:Vero Cells