High-performance liquid chromatographic determination of chloramphenicol and four analogues using reductive and oxidative electrochemical and ultraviolet detection.
A high-performance liquid chromatographic procedure is described for the separation, quantitation and identification of chloramphenicol, dehydrochloramphenicol, nitrophenylaminopropanedione, nitrosochloramphenicol and aminochloramphenicol. An isocratic reversed-phase system with ultraviolet and electrochemical detectors in tandem was assembled and used. The system was constructed with special accommodation to enable us to use the electrochemical detector in both reductive and oxidative modes. Retention characteristics, hydrodynamic voltammograms under reductive and oxidative conditions and ultraviolet absorbance are reported. Applicability of the procedure to biological fluids was demonstrated by separation and detection of chloramphenicol after incubation with human blood.
D002701:Chloramphenicol / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet
D002701:Chloramphenicol