[Determination of the biological activity of Clostridium difficile toxins in in vivo and in vitro experiments].
The biological activity of the filtrates of 29 C. difficile strains was studied in vivo (suckling white mice) and in vitro (cell cultures of different species and origin). The action of the filtrates on the experimental models in vivo was evaluated from the cytotoxic effect index, while in vitro the intensity of the cytotoxic effect was evaluated from the percentage of dead cells in the monolayer. The results of the comparative determination of toxicity characteristics in vivo and in vitro demonstrated that cell cultures were more sensitive experimental models than suckling white mice. The use of cell cultures permitted the quantitative evaluation of the cytotoxic activity of the filtrates under study, as well as the detection of their cell-directed action at minimal concentrations.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003013:Clostridium; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000633:toxicity*; D006801:Humans; D051379:Mice; D013045:Species Specificity
D004768:Enterotoxins
Opredeleneie biologicheskoĭ aktivnosti toksinov Clostridium difficile v éksperimentakh in vivo i in vitro.