title: Cloning and sequencing of the medium-chain S-acyl fatty acid synthetase thioester hydrolase cDNA from rat mammary gland.
abstract: cDNA clones coding for the medium-chain S-acyl fatty acid synthetase thioester hydrolase (thioesterase II) from rat mammary gland were identified in a bacteriophage lambda gt11 library and their nucleotide sequences were determined. The predicted coding region spans 263 amino acid residues and includes a sequence identical with that of a peptide derived from the enzyme active site. The rat thioesterase II cDNA sequence exhibits homology with that of a thioesterase found in duck uropygial glands.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats; D013869:Thiolester Hydrolases / Q000235:genetics*
chemical_list: D004247:DNA; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase