[A method of determining NADH-methemoglobin reductase activity using amino derivatives of o-benzoquinone].
Rate of methemoglobin reduction was increased in erythrocyte hemolysates in presence of 4-N-(p-sulfanilino)-5-methoxy-1.2-benzoquinone. Kinetic parameters of total reaction and of individual steps showed that the substance and other amino-derivatives of ortho-benzoquinone could be used for estimation of methemoglobin reductase activity.
D000818:Animals; D001557:Benzenesulfonates; D016227:Benzoquinones; D042966:Cytochrome-B(5) Reductase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D007202:Indicators and Reagents; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000097:blood*; D010084:Oxidation-Reduction; D011809:Quinones; D011817:Rabbits
C016764:4-N-(p-sulfoaniline)-5-methoxy-1,2-benzoquinone; D001557:Benzenesulfonates; D016227:Benzoquinones; D007202:Indicators and Reagents; D011809:Quinones; D009247:NADH, NADPH Oxidoreductases; D042966:Cytochrome-B(5) Reductase; C025225:2-benzoquinone
Opredelenie aktivnosti NADH-metgemoglobinreduktazy s pomoshch'iu aminoproizvodnykh ortobenzokhinona.