title: Cell pairs isolated from adult guinea pig and rat hearts: effects of [Ca2+]i on nexal membrane resistance.
abstract: Cell pairs isolated from adult rat and guinea pig ventricles were used to study the resistance of the nexal membrane, rn. Each cell of a cell pair was connected to a voltage-clamp circuit to obtain simultaneous whole-cell, tight-seal recordings. With this technique, rn was determined under experimental conditions aimed at primarily modifying [Ca2+]i. Moderate changes in [Ca2+]i (produced by trains of depolarizing voltage-clamp pulses activating the slow inward current, or alterations in [Ca2+]o from 0.5 to 10 mM), resulted in no change in rn for normally coupled cell pairs (rn = 5 M omega), but small and reversible changes in slightly uncoupled preparations (rn greater than or equal to 50 M omega). Large increases in rn developed with substantial elevations in [Ca2+]i (secondary to [Na+]o-withdrawal, exposure to strophanthidin in conjunction with isi, or Ca2+-dialysis). Increases in rn brought about via elevation in [Ca2+]i always were accompanied by cell shortening consistent with a sustained contracture. The current-voltage relationship of the nexal membrane was ohmic regardless of whether rn was low (control) or elevated (after increasing [Ca2+]i).
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D004553:Electric Conductivity; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D009206:Myocardium / Q000166:cytology; D011690:Purkinje Fibers / Q000502:physiology; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D016276:Ventricular Function
chemical_list: D002110:Caffeine; D012964:Sodium; D002118:Calcium