Purification of human factor XII from plasma using zinc chelate affinity chromatography.
Human factor XII (Hageman Factor) was isolated from human plasma to apparent homogeneity using a four step procedure with yields up to 30%. The method, which is more rapid than the current conventional procedures, consists of a 25-50% ammonium sulfate fractionation, two affinity chromatography steps using Zinc Chelate Sepharose, followed by gel filtration. The isolated zymogen factor XII was a single protein component when examined by SDS PAGE with a Mr of 80,000. Activation of zymogen factor XII to its active enzymatic forms by kallikrein resulted in factor XIIa and factor XIIf as observed with factor XII purified by other procedures.
D000645:Ammonium Sulfate; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005174:Factor XII / Q000302:isolation & purification*; D006801:Humans; D007610:Kallikreins; D008970:Molecular Weight; D012685:Sepharose / Q000031:analogs & derivatives
C030523:zinc chelate-sepharose; D005174:Factor XII; D012685:Sepharose; D007610:Kallikreins; D000645:Ammonium Sulfate