Three-dimensional reconstruction of a human metaphase chromosome from electron micrographs.
A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was fairly homogeneous within the chromatids and neither a hollow nor a dense core was observed. The distribution and course of fibers observed are most consistent with a looping model of chromosome structure.
D000465:Algorithms; D002877:Chromosomes, Human / Q000648:ultrastructure*; D003198:Computer Simulation; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000648:ultrastructure; D008677:Metaphase; D008854:Microscopy, Electron / Q000379:methods; D008961:Models, Structural