Electrophoretic resolution of two rat class I alloantigens expressed on (WF X F344)F1 liver cells in primary culture.
Polyvalent alloantisera, prepared by reciprocal immunization of F344 (RT1lv1 haplotypes) and WF (RT1u haplotype) rats, as well as monoclonal antibodies, were used to immunoprecipitate class I alloantigens from detergent extracts of monolayer cultures of 35S-methionine-labelled liver cells. Two-dimensional IEF/SDS-PAGE gel analysis resolved the RT1.Alv1 and RT1.Au class I antigens expressed on the liver cells in culture.
D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008099:Liver / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D011920:Rats, Inbred WF
D006649:Histocompatibility Antigens