Short-chain fatty acids in seven sterile thioglycollate media.
Short-chain fatty acids (SCFAs) were analysed by gas chromatography in 7 sterile fluid thioglycollate media. The total SCFA concentration varied from 250 mumol/l to 1720 mumol/l. Acetic acid (52.0%-91.3% of total SCFA concentration) and n-butyric acid (5.8%-48.0% of total concentration) were the main acids in all sterile media; low concentrations of propionic, isobutyric, isovaleric, and n-valeric acid were present in most media. In studies of bacterial metabolism, or when analyses of SCFAs are used for identification of bacteria, testing of the sterile medium is necessary.
D002849:Chromatography, Gas; D003470:Culture Media / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis*; D013864:Thioglycolates
D003470:Culture Media; D005227:Fatty Acids; D013864:Thioglycolates