title: Polyamine metabolism in filarial worms.
abstract: The human and animal filarial parasites Onchocerca volvulus, Dirofilaria immitis, Brugia patei and Litomosoides carinii contained low levels of putrescine but much higher levels of spermidine and spermine as estimated by ion-pair high pressure liquid chromatography; N-acetylated polyamines were present only in minute amounts. Enzyme activities of ornithine decarboxylase (EC 4.1.1.17) and arginine decarboxylase (EC 4.1.1.19), respectively, were not detectable. Experiments carried out with O. volvulus and D. immitis demonstrated the uptake and bioconversion of labeled polyamines. There is evidence for the existence of a complete reverse pathway generating putrescine from spermidine and spermine, respectively, in both worms. N-Acetylating enzyme activities were detected in 100,000 X g preparations of homogenates from D. immitis which were capable to acetylate putrescine, spermidine and spermine. Long term incubation of the worms in the presence of labeled polyamines resulted in the excretion of putrescine and N-acetylputrescine.
mesh_terms: D000818:Animals; D002009:Brugia / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004183:Dirofilaria immitis / Q000032:analysis / Q000378:metabolism; D005260:Female; D005370:Filarioidea / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D008297:Male; D009854:Onchocerca / Q000032:analysis / Q000378:metabolism; D011073:Polyamines / Q000032:analysis / Q000378:metabolism*; D011700:Putrescine / Q000032:analysis / Q000378:metabolism; D013095:Spermidine / Q000032:analysis / Q000378:metabolism; D013096:Spermine / Q000032:analysis / Q000378:metabolism
chemical_list: D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine