Elastase activity in capillary and aortic endothelial cells.
Tissue cultured bovine capillary and aortic endothelial cells were examined for cellular and secreted proteinase activity. Cell-lysates from both sources showed significant elastase activity but differed in the level of activity and the pH-optimum and inhibition profile. Secretion of elastase was only detected using aortic cells. Tumour angiogenesis factor (TAF), heparin or 12-0-tetradecanoyl-phorbol-acetate (TPA) had no effect on the levels of cell-associated or secreted elastase.
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000201:enzymology*; D002196:Capillaries / Q000166:cytology / Q000201:enzymology*; D002417:Cattle; D046508:Culture Techniques; D004727:Endothelium / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010196:Pancreatic Elastase / Q000032:analysis*; D013379:Substrate Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D043925:Angiogenesis Inducing Agents; D010196:Pancreatic Elastase; D013755:Tetradecanoylphorbol Acetate