title: Proteoglycan metabolism associated with mouse metanephric development: morphologic and biochemical effects of beta-D-xyloside.
abstract: Morphology and de novo incorporation of [35S]sulfate into proteoglycans were studied in fetal mouse kidneys at the onset of organogenesis. Branching morphogenesis and nephron development in organ culture and in vivo were associated with de novo synthesis of chondroitin-SO4 and heparan-SO4 proteoglycans. The role of proteoglycan metabolism in metanephrogenesis was then studied by analysis of the effects of p-nitrophenyl-beta-D-xylopyranoside (beta-D-xyloside) on renal development and proteoglycan metabolism. Incubation of fetal kidneys in beta-D-xyloside at concentrations of 1.0 and 0.5 mM, but not at 0.1 mM, caused inhibition of ureteric branching and markedly diminished synthesis of a large Mr 2.0 X 10(6) Da chondroitin-SO4 proteoglycan. Incorporation of [35S]sulfate was stimulated at all beta-D-xyloside concentrations, reflecting synthesis of xyloside initiated dermatan-35SO4 chains. In contrast to dramatic effects on chondroitin-SO4 synthesis and ureteric branching, beta-D-xyloside had no effect on heparan-SO4 synthesis or on development of the glomerulus and glomerular basement membrane. We thus characterize the proteoglycans synthesized early in the course of renal organogenesis and describe observations which suggest an association between metabolism of chondroitin-SO4 proteoglycan and development of the ureter.
mesh_terms: D000818:Animals; D005314:Embryonic and Fetal Development; D006027:Glycosides / Q000494:pharmacology*; D006651:Histocytochemistry; D007668:Kidney / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure; D007678:Kidney Glomerulus / Q000196:embryology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009024:Morphogenesis / Q000187:drug effects; D009924:Organ Culture Techniques; D011509:Proteoglycans / Q000378:metabolism*; D013462:Sulfur Radioisotopes
chemical_list: D006027:Glycosides; D011509:Proteoglycans; D013462:Sulfur Radioisotopes; C010807:xylosides