title: New approach to the rapid optimisation of conditions for the isocratic separation of multicomponent mixtures.
abstract: Despite the rapid advances in chromatographic technology, the development of high-performance liquid chromatographic methods remains expensive with respect to both time and materials. A new approach is proposed here which is suitable for the investigation of eluents and packings for the resolution of multicomponent samples. This is based upon the use of a small column to mimic the retentive characteristics of isocratic analytical systems but on a greatly reduced time scale. The general utility of this approach is illustrated by three applications, using data for purines and pyrimidines on both reversed-phase and ion-exchange systems.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D009711:Nucleotides / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D011725:Pyridines / Q000302:isolation & purification; D011743:Pyrimidines / Q000302:isolation & purification
chemical_list: D004338:Drug Combinations; D009711:Nucleotides; D004364:Pharmaceutical Preparations; D011725:Pyridines; D011743:Pyrimidines