title: Dog secretin: sequence and biologic activity.
abstract: Secretin was purified from the first 50 cm of proximal intestine of a single dog. The purification sequence included extraction in acid-ethanol, differential precipitation with acetone, Sephadex gel filtration and three successive HPLC steps. Dog secretin has the following sequence: HSDGTFTSELSRLRESARLQRLLQGLV. The underlined amino acid represents a substitution of Glu for Asp at position 15 from the NH2-terminus of pig secretin. Dog secretin is equal in potency to pig secretin in stimulating pancreatic ductal flow in a rat bioassay system.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D004285:Dogs; D007421:Intestine, Small / Q000502:physiology*; D010189:Pancreatic Juice / Q000187:drug effects / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D012633:Secretin / Q000302:isolation & purification* / Q000494:pharmacology; D013552:Swine
chemical_list: D012633:Secretin