Effect of vanadate of PHA-induced proliferation of human lymphocytes from young and old subjects.
The effect of sodium orthovanadate on mitogen-induced proliferation of lymphocytes from young and old human subjects is reported. We found that vanadate is not mitogenic per se; it has an inhibitory effect during the first 3 days of culture, when both differentiation and proliferation take place; it enhances DNA synthesis, acting as a co-mitogen, in the following days of culture, when proliferation prevails. In spite of the fact that lymphocytes from the two groups differ in their responsiveness to PHA and in the activity of (Na+,K+)ATPase, no difference was found as for the effects of vanadate.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013997:Time Factors; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
D010835:Phytohemagglutinins; D014639:Vanadium; D014638:Vanadates; D004247:DNA