Dye and electrotonic coupling between cultured hippocampal neurons.
Electrotonic coupling was examined between hippocampal neurons in dissociated cell cultures. Injections of Lucifer yellow indicated that approximately 20% of the neurons were dye-coupled. Propionate, which causes cytoplasmic acidification, significantly decreased dye coupling. Simultaneous intracellular recordings from pairs of cultured neurons directly demonstrated electrotonic coupling. Therefore dissociated cell cultures can be used as a model system for the analysis of hippocampal electrotonic coupling.
D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D004558:Electric Stimulation; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000166:cytology* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007365:Intercellular Junctions / Q000378:metabolism / Q000502:physiology*; D007546:Isoquinolines; D008564:Membrane Potentials; D009474:Neurons / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats
D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow