Differentiation of gut endoderm in dependence of the notochord.
Presumptive intraembryonic endoderm, either isolated or together with adhering mesoderm, from 19-h chick embryos, was grafted to the coelom of 50-h host embryos. The viability of such grafts was low and endodermal differentiation was poor. In a second series the endoderm (with or without adhering mesoderm) was combined with a fragment of notochordal tissue from 48-60-h donor embryos. Then the recovery was much higher, notably after longer periods of in vivo culture. After 10 days of cultivation well-developed entero-endocrine (argyrophilic) cells were found among the regular enterocytes in both series.
D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D004064:Digestive System / Q000196:embryology*; D004622:Embryo, Mammalian / Q000502:physiology*; D004625:Embryo, Nonmammalian; D004707:Endoderm / Q000166:cytology* / Q000637:transplantation; D004848:Epithelium / Q000196:embryology; D006085:Graft Survival; D008648:Mesoderm / Q000166:cytology; D009672:Notochord / Q000502:physiology* / Q000637:transplantation; D013997:Time Factors