The effect of acetylcholine on chloride transport across the mouse lacrimal gland acinar cell membranes.
The mechanisms of Cl- transport and the effects of acetylcholine (ACh) and electrochemical Cl- potential changes across the basolateral plasma membrane on intracellular Cl- activity in the acinar cells of isolated mouse lacrimal glands were studied using double-barreled Cl- -selective microelectrodes. In the resting state, the basolateral membrane potential (Vm) was about -40 mV and intracellular Cl- activity was about 35 mmol/l. Addition of ACh (10(-9) approximately 10(-6) mol/l) hyperpolarized Vm and decreased the Cl- activity in a dose-dependent manner. ACh (10(-6) mol/l) hyperpolarized Vm by 20 mV and decreased the cytosolic Cl- activity with an initial rate of 16.0 mmol/l X min. Reduction of the perfusate Cl- concentration to 1/9 control depolarized Vm and decreased cytosolic Cl- activity at a rate of 1.9 mmol/l X min. A Vm hyperpolarization of 20 mV produced by DC injection to the adjacent cell decreased Cl- activity at a rate of 4.6 mmol/l X min. DIDS (1 mmol/l) hyperpolarized Vm by 8 mV with little change in Cl- activity and increased the input resistance of the cells by 25%. DIDS decreased the rate of change in Cl- activity induced by low-Cl- Ringer to 35% of control, but had no effect on the ACh-evoked decrease in the Cl- activity. Furosemide (1 mmol/l) slightly hyperpolarized Vm and decreased Cl- activity at a slow rate but affected Cl- movements induced by ACh or low-Cl- Ringer only slightly. Cl- uptake into the cells was inhibited partially by furosemide.(ABSTRACT TRUNCATED AT 250 WORDS)
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D004558:Electric Stimulation; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D007765:Lacrimal Apparatus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice
D002712:Chlorides; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D005665:Furosemide; D000109:Acetylcholine; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid