Release of individual enzymes from guinea pig pancreas following stimulation with CCK-8 or CCK-33.
This paper compares the effects of prolonged stimulation with CCK-33 or CCK-8 on the secretion of individual enzymes from the pancreas of the anaesthetised guinea-pig. Quantitative analysis of the individual secretory proteins was achieved using a newly developed, reversed-phase, high-performance, liquid chromatography technique. Using this system, it was possible to separate and quantify each of the nine major proteins present in a small sample of pancreatic juice in 40 min. Because spontaneous pancreatic secretion was very slow in the anaesthetised guinea-pig, the cholecystokinin (CCK) peptides were administered against a background infusion of secretin. Secretin alone produced a small rise in total protein release with the secretion of all individual proteins being increased in parallel. Doses of CCK-33 and CCK-8 which increased total protein secretion by a similar amount had contrasting effects on the release of individual proteins. CCK-33 produced a parallel release of all proteins over a period of 2 h. By contrast, after 1-h stimulation with CCK-8, the secretion of proteins became nonparallel. These data suggest that CCK-33 and CCK-8 may provoke slightly different intracellular responses within the pancreatic acinar cell.
D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism; D012844:Sincalide / Q000494:pharmacology*; D013997:Time Factors; D014362:Trypsinogen / Q000378:metabolism
D011506:Proteins; D014362:Trypsinogen; D002766:Cholecystokinin; D012844:Sincalide