Utilization of synthetic peptides for the study of calcitonin and biosynthetic precursors for calcitonin.
By using synthetic peptides and a library of monoclonal anti-peptide antibodies, we have developed a panel of techniques that allow the dissection of circulating immunoreactive calcitonin in the serum. C Cells of the thyroid were found to release both mature calcitonin and biosynthetic intermediates in the circulation. Finally, these products were found to circulate as heterogenous molecular species. A methodology for the standardization of the measurement of calcitonin is proposed in the form of a two-site immunoradiometric assay specific for mature calcitonin.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002116:Calcitonin / Q000097:blood* / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011498:Protein Precursors / Q000097:blood* / Q000276:immunology
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D011498:Protein Precursors; C033183:preprocalcitonin; D002116:Calcitonin