title: The establishment of the embryonic-abembryonic axis in the mouse embryo.
abstract: The influence of cell division order on the establishment of the embryonic-abembryonic axis (EA axis) of the mouse embryo was investigated. Aggregate embryos were constructed in which a labelled cell (or pair of cells) was combined with a group of unlabelled cells all of which were up to one cell cycle earlier or later in their progress through development to the blastocyst stage. The aggregates were cultured first to the nascent blastocyst stage and then to the expanded blastocyst stage. The positions of the progeny of the labelled cells in relation to the nascent blastocoel and to the orientation of the embryonic-abembryonic axis were recorded. It was concluded that cell division order does influence the establishment of the EA axis, early dividing cells tending to be associated with the nascent blastocoel and the site of the nascent blastocoel tending to mark the site of the abembryonic pole. However, the influence of division order was diminished by a requirement for intercellular cooperation during blastocoel formation and by a counteracting influence of division order arising from its effects on the allocation of cells to the inner cell mass.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002449:Cell Aggregation; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D004627:Embryonic Induction; D005314:Embryonic and Fetal Development; D051379:Mice; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence; D014327:Trophoblasts / Q000166:cytology