Removal of human polymorphonuclear leukocyte surface sialic acid inhibits reexpression (or recycling) of formyl peptide receptors. A possible explanation for its effect on formyl peptide-induced polymorphonuclear leukocyte chemotaxis.
Removal of surface sialic acid specifically inhibits human polymorphonuclear leukocyte (PMN) chemotactic responses to N-formyl-methionyl-leucyl-phenylalanine (FMLP). Neuraminidase-treated (NT)-PMN bound and internalized [3H]FMLP (used as receptor marker) as well as normal PMN. NT-PMN, however, retained more [3H]FMLP-associated radioactivity than normal PMN. Subcellular fractionation studies demonstrated that NT-PMN retained more sedimentable (100,000 X G for 180 min) [3H]FMLP-associated radioactivity within light Golgi-containing fractions than normal PMN. Furthermore, NT-PMN exhibited a defect in their ability to reexpress (or recycle) a population of FMLP receptors. Abnormal receptor recycling was associated with inhibition of FMLP-induced PMN chemotaxis. Thus, it appears that recycling of formyl peptide receptors may be necessary for optimal PMN chemotactic responses to FMLP. We postulate that removal of PMN surface sialic acid inhibits FMLP-induced PMN chemotaxis by blocking the reexpression (or recycling) of a population of formyl peptide receptors, perhaps by preventing trafficking of desialated receptors through a light Golgi pathway.
D000345:Affinity Labels; D002451:Cell Compartmentation; D002634:Chemotaxis, Leukocyte; D004705:Endocytosis; D005089:Exocytosis; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000502:physiology*; D009439:Neuraminidase; D009504:Neutrophils / Q000502:physiology*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000378:metabolism*; D012794:Sialic Acids / Q000502:physiology*; D013997:Time Factors
D000345:Affinity Labels; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D012794:Sialic Acids; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009439:Neuraminidase