Loss of phospholipid asymmetry in dilauroylphosphatidylcholine induced plasma membrane vesicles from human platelets.
Incubation of human platelets with unilamellar vesicles composed of dilauroylphosphatidylcholine (DLPC) induces shedding of small vesicular structures from the platelet plasma membrane. No significant cell lysis is observed during the process of shedding. Isolated spicules contain the major membrane glycoproteins, Ib, IIb, and IIIa, which are used to define the sidedness of the spicule membrane. These glycoproteins are completely susceptible to chymotrypsin treatment, whereas cytoskeletal proteins are inaccessible towards this enzyme. This demonstrates that the spicule membranes have a right-side-out orientation in as far as membrane proteins are concerned. Isolated spicules were 30-fold more active than platelets in stimulating prothrombin conversion to thrombin by the prothrombinase complex (factors Xa, Va and Ca2+). The increased prothrombinase activity reflects an increased amount of phosphatidylserine in the outer leaflet of the spicule membrane. Protein analysis of platelet spicules and native platelets reveals a number of differences, the most conspicuous of which is the virtual absence of myosin in the spicule preparations. It is proposed that a lack of myosin produces a different cytoskeletal organization in the spicules. This enables phosphatidylserine to become exposed at the outer surface of the spicule membrane.
D001792:Blood Platelets / Q000648:ultrastructure*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D008081:Liposomes; D008563:Membrane Lipids / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood; D008854:Microscopy, Electron; D009218:Myosins / Q000097:blood; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010718:Phosphatidylserines / Q000097:blood; D010743:Phospholipids / Q000097:blood*; D013925:Thromboplastin / Q000097:blood; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D008081:Liposomes; D008563:Membrane Lipids; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D010743:Phospholipids; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; C015153:1,2-dilauroylphosphatidylcholine; D013925:Thromboplastin; D009218:Myosins