Techniques for the detection of lipids in high-performance liquid chromatography.
The use of an infrared detector for the detection of lipids in both normal and reversed-phase chromatography is demonstrated. A system is also described for the derivatisation of lipid molecules containing a C = O group to form the hydroxamic acids and their separation and detection as the iron(III) hydroxamates.
D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids / Q000032:analysis / Q000138:chemical synthesis; D008055:Lipids / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D006877:Hydroxamic Acids; D008055:Lipids