In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum.
A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.
D000818:Animals; D001792:Blood Platelets / Q000276:immunology / Q000502:physiology*; D007106:Immune Sera / Q000494:pharmacology*; D066298:In Vitro Techniques; D008533:Megakaryocytes / Q000276:immunology / Q000502:physiology*; D051381:Rats
D007106:Immune Sera