Acinar redistribution and heterogeneity in transport of the organic cation rhodamine B in rat liver.
We studied a possible acinar heterogeneity in the transport of organic cations, using rhodamine B as model compound. Employing perfusions of isolated rat livers in the ante- and retrograde mode and quantitative fluorescence microscopy, Zones 1 and 3 were shown to be equally efficient in taking up rhodamine B. Ten minutes after injection in an antegrade perfusion, 95% of the dose was localized in the portal half of the acinus. Fifty minutes later, however, the amount of rhodamine B in Zone 1 had been reduced to 23%; 30 and 31% were in Zones 2 and 3, respectively, and the medium concentration was doubled. Thus, unchanged rhodamine B appeared to be transported downstream within the liver, either via the medium or directly from cell to cell, finally resulting in a relatively higher rhodamine B concentration in Zone 3. To obtain additional data, we designed a perfusion setup in which the zones could be studied separately. In both zones, the amount excreted into the medium was about 30 times the amount excreted into bile. Intracellular sequestration of rhodamine B and the rate constant for sinusoidal secretion were higher in Zone 3, while the sinusoidal uptake rates were equal; biliary excretion was higher in Zone 1. Acinar distribution changed with time because rhodamine B, primarily accumulated in Zone 1, was secreted into the sinusoids and taken up again by downstream cells. The finally higher rhodamine B concentration in Zone 3 was caused by a zonal heterogeneity in intracellular sequestration and sinusoidal secretion of rhodamine B.
D000818:Animals; D001646:Bile / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012235:Rhodamines / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014966:Xanthenes / Q000493:pharmacokinetics*
D005456:Fluorescent Dyes; D012235:Rhodamines; D014966:Xanthenes; C029773:rhodamine B