Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus.
Thirteen adherent human non-lymphocyte cell lines were tested for their susceptibility to infection by human immunodeficiency virus. Productive infection could be demonstrated in three of five colorectal carcinoma cell lines examined; the other eight human non-lymphocyte cell lines were uninfectible. A susceptible colon carcinoma cell line (HT29), as well as normal colonic mucosa, was shown to contain a 3.0-kilobase species of poly(A)+ CD4 RNA, whereas uninfectible colon carcinoma and rhabdomyosarcoma cell lines synthesized no detectable T4 RNA. A persistently infected colon carcinoma cell line was established that continued to produce progeny human immunodeficiency virus for more than 10 weeks postinfection.
D002460:Cell Line; D003110:Colonic Neoplasms / Q000382:microbiology*; D004198:Disease Susceptibility; D006678:HIV / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D009369:Neoplasms / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012004:Rectal Neoplasms / Q000382:microbiology*