Leukocyte depletion of red cell units at the bedside by transfusion through a new filter.
Standard packed red cell (PRC) units can be depleted of leukocytes and platelets if they are transfused through a blood administration set in which the usual 170-mu filter has been replaced by a leukocyte removal filter (Sepacell R-500). During a 6-month period, 1550 PRC units were transfused through this filter in 611 transfusions to 80 multitransfused patients with thalassemia who had had a patient reaction rate (PRR) of 63 percent and a transfusion reaction rate (TRR) of 13 percent when given standard PRC or buffy-coat-depleted PRC. When given filtered PRC, PRR and TRR became 3.7 percent and 0.5 percent, respectively. The effectiveness of the filter was also evaluated in vitro. By filtering 2 standard PRC units through the same filter, median values (and ranges) for red cell recovery and for residual leukocytes and platelets were 87 percent (83-92), 6.1 X 10(6) (0-100), and 2.7 X 10(9) (0.6-9.7), respectively. Although refinements are needed to improve standardization of the filter and to increase red cell recovery (which is low when 1 unit is filtered through one filter) and blood administration rate, the ability to provide leukocyte-free red cells prepared at the bedside for virtually all recipients appears to be a realistic goal.
D001769:Blood; D001803:Blood Transfusion / Q000295:instrumentation / Q000379:methods*; D017707:Erythrocyte Transfusion; D005069:Evaluation Studies as Topic; D005334:Fever / Q000209:etiology / Q000517:prevention & control; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology; D007937:Leukapheresis / Q000295:instrumentation / Q000379:methods*; D007958:Leukocyte Count; D010976:Platelet Count; D010983:Plateletpheresis; D065227:Transfusion Reaction; D014462:Ultrafiltration / Q000295:instrumentation*