Rapid analysis of beta-phenylethylamine in tissues and body fluids utilizing pentafluorobenzoylation followed by electron-capture gas chromatography.
A rapid, sensitive gas chromatographic procedure for analysis of beta-phenylethylamine is reported. The procedure involves extraction with a liquid ion-pairing compound, back-extraction with HCl, basification and reaction with pentafluorobenzoyl chloride under aqueous conditions. The pentafluorobenzoyl derivative of beta-phenylethylamine is then separated and analyzed on a gas chromatograph equipped with a capillary column and an electron-capture detector. The procedure produces a derivative which has good chromatographic properties and a high degree of stability. The method has been applied to analysis of beta-phenylethylamine in a variety of tissues and body fluids.
D000818:Animals; D001565:Benzoates; D001826:Body Fluids / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods*; D003866:Depressive Disorder / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry; D009206:Myocardium / Q000378:metabolism; D010627:Phenethylamines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D005464:Fluorobenzenes; D005466:Fluorocarbons
D001565:Benzoates; D010627:Phenethylamines; C014737:pentafluorobenzoyl chloride; D005464:Fluorobenzenes; D005466:Fluorocarbons; C029261:phenethylamine