1-O-n-octyl-beta-D-glucopyranoside as a competitive inhibitor of Na+-dependent D-glucose cotransporter in the small intestine brush-border membrane.
1-O-n-Octyl-beta-D-glucopyranoside is a competitive inhibitor of the Na+-dependent D-glucose uptake into rabbit, rat and human intestinal brush-border membrane vesicles. The lack of effect on the equilibrium uptake demonstrates that the detergent does not act by rupturing the vesicles; no membrane leakiness was apparent at the concentrations of octylglucopyranoside used, since D-glucose uptake is not inhibited even in the absence of the Na+ gradient (in K+ solution). There is a competitive interaction between octylglucopyranoside and D-glucose, as shown by Dixon and by Hunter and Down plots. The selectivity of the detergent effect is confirmed by its modest influence on amino acid uptake.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors*; D010695:Phlorhizin / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012964:Sodium / Q000494:pharmacology*; D013863:Thioglucosides / Q000494:pharmacology*; D013865:Thioglycosides / Q000494:pharmacology*
D000596:Amino Acids; D009004:Monosaccharide Transport Proteins; D013863:Thioglucosides; D013865:Thioglycosides; C042989:n-octyl-beta-D-thioglucopyranoside; D012964:Sodium; D010695:Phlorhizin; D005947:Glucose