Peroxide(s) of vanadium: a novel and potent insulin-mimetic agent which activates the insulin receptor kinase.
The actions of insulin, vanadate (V) and hydrogen peroxide (H2O2) on IGF-II binding and insulin receptor tyrosine kinase activity were studied in rat adipocytes. Incubating adipocytes with a combination of V plus H2O2 resulted in a potent synergistic effect on both the increase in IGF-II binding and the activation of the insulin receptor kinase. Catalase, which removes H2O2, abolished this synergism if added at the time of mixing of V plus H2O2 but not if added 10 min. later, suggesting that the formation of peroxide(s) of vanadate generated a potent insulin mimicker. The data support a critical role for the insulin receptor kinase in insulin action. The novel insulin-mimetic compound, a presumed peroxide of vanadate, could prove useful for investigating insulin action and may be valuable for treating insulin resistance.
D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D008297:Male; D010545:Peroxides / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism*; D014639:Vanadium / Q000494:pharmacology*
D010545:Peroxides; D014639:Vanadium; D007335:Insulin-Like Growth Factor II; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin