Inhibition of sodium-dependent transport systems in rat renal brush-border membranes with N,N'-dicyclohexylcarbodiimide.
Treatment of rat renal brush-border membrane vesicles with N,N'-dicyclohexylcarbodiimide (DCCD) causes irreversible inhibition of the Na+-coupled transport systems for D-glucose, L-phenylalanine, L-glutamate, and sulfate. The DCCD-reactive side groups of these transport systems differ in their sensitivity towards DCCD and protection by substrates. The D-glucose and L-glutamate transporters cannot be protected by their substrates. In contrast, Na+ protects the transport systems for L-phenylalanine and sulfate from inactivation by DCCD. The data suggest covalent modification by DCCD of D-glucose and L-glutamate transporters apart from their substrate binding sites and of L-phenylalanine and sulfate transporters within their Na+-binding regions.
D026905:Amino Acid Transport Systems; D001692:Biological Transport / Q000187:drug effects; D002234:Carbodiimides / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism*; D027682:Cation Transport Proteins; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D000077003:Sodium Sulfate Cotransporter; D013431:Sulfates / Q000378:metabolism; D027981:Symporters
D026905:Amino Acid Transport Systems; D002234:Carbodiimides; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D005971:Glutamates; D009004:Monosaccharide Transport Proteins; D000077003:Sodium Sulfate Cotransporter; D013431:Sulfates; D027981:Symporters; D010649:Phenylalanine; D004024:Dicyclohexylcarbodiimide; D012964:Sodium; D005947:Glucose