title: The effect of dimethylsulphoxide on the microtubular system of the mouse oocyte.
abstract: The effect of dimethylsulphoxide (DMSO) on the organization of the microtubular system of the mouse oocyte has been examined. Exposure to DMSO causes the immediate appearance of multiple, cold-resistant microtubular asters associated with the foci of pericentriolar material (PCM) normally present in the oocyte. More prolonged exposure to DMSO leads to progressive disassembly of the spindle, and as a result dispersal of the chromosomes and polar PCM foci occurs, and tubulin polymerization becomes confined to PCM-organized asters. Those astral microtubules located between the PCM foci and the cortex of the oocyte appear to be particularly stable, resulting in the development of lengthening radial bundles of microtubules between the PCM and the surface and the progressive movement of the PCM foci towards the centre of the cell. In contrast, after activation of the oocyte the microtubules generated in the presence of DMSO remain located in a cortical mesh. The effects of DMSO do not appear to be fully reversible in most oocytes. We discuss the implications of these results both for the cytoplasmic organization of the oocyte and zygote, and for the attempts at cryopreservation of human oocytes for therapeutic use in infertility programmes.
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology; D002843:Chromatin / Q000378:metabolism; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008870:Microtubules / Q000187:drug effects*; D015739:Nocodazole; D009865:Oocytes / Q000187:drug effects*; D014404:Tubulin / Q000378:metabolism
chemical_list: D000995:Antitubercular Agents; D001562:Benzimidazoles; D002843:Chromatin; D003572:Cytochalasins; D014404:Tubulin; D015638:Cytochalasin D; D015739:Nocodazole; D004121:Dimethyl Sulfoxide