title: Separation of canavanine and canaline by high performance liquid chromatography.
abstract: The plant amino acid canavanine and its hydrolytic product canaline were successfully separated and identified by Reverse Phase High Performance Liquid Chromatograph (RP-HPLC). This procedure was used to demonstrate the arginase-mediated cleavage of canavanine to yield canaline and urea, and the subsequent formation of a Schiff's base complex between canaline and pyridoxal phosphate. Both aforementioned reactions were demonstrated by RP-HPLC.
mesh_terms: D000613:Aminobutyrates / Q000302:isolation & purification*; D001119:Arginase; D002172:Canavanine / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae; D000455:Medicago sativa; D010945:Plants, Edible / Q000032:analysis*; D010946:Plants, Medicinal; D009764:o-Phthalaldehyde
chemical_list: D000613:Aminobutyrates; D002172:Canavanine; C001926:canaline; D009764:o-Phthalaldehyde; D001119:Arginase