Influence of 5-hydroxytryptamine on crypt cell production rate of human duodenal mucosa cultured in vitro.
The effect of 5-hydroxytryptamine (5-HT) on the crypt cell production rate in normal human duodenal mucosa cultured in vitro was studied using a stathmokinetic technique with crypt microdissection. The addition of 5-HT (2 mg/ml) to the culture medium significantly increased the crypt cell production rate (p less than 0.001), suggesting that this amine may have a trophic effect on crypt cells in vitro. Increased synthesis and release of 5-HT from enterochromaffin cells in patients with coeliac disease may increase the crypt cell production rate and lead to a flattened small intestinal mucosa.
D046508:Culture Techniques; D004386:Duodenum / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008938:Mitosis / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013997:Time Factors
D012701:Serotonin