Detection of anomalies in cell-surface carbohydrates on thrombasthenic platelets using 125I-labeled lectins.
The composition of carbohydrates on the surface of platelets from a patient with Glanzmann's thrombasthenia and from seven normal donors were determined and compared. To this end, binding studies were performed using nine different purified 125I-labeled lectins; Concanavalin A, P-Phytohaemagglutinin, Wheat Germ Agglutinin, Dolichos biflorus, Pisum sativum, Ricinus communis II Agglutinin, Tetragonolobus purpureus, Lens culinaris and Soybean Agglutinin. These studies show that thrombasthenic platelets bear significantly decreased numbers of receptors for Concanavalin A and Lens culinaris, both with a specificity for D-mannose, and Ricinus communis II, with specificity for D-galactose. There were no detectable differences in the numbers of other lectin receptors. These results provide further evidence of molecular defects in thrombasthenic platelets. Moreover, the use of 125I-labeled lectins, as shown here, provides a fast and reliable technique for identifying abnormalities in the carbohydrate composition on the surface of platelets in various thrombopathies.
D001791:Blood Platelet Disorders / Q000097:blood*; D001792:Blood Platelets / Q000032:analysis*; D002241:Carbohydrates / Q000097:blood*; D003208:Concanavalin A / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007700:Kinetics; D037102:Lectins / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D010835:Phytohemagglutinins / Q000378:metabolism; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000378:metabolism; D030262:Soybean Proteins; D013915:Thrombasthenia / Q000097:blood*; D014909:Wheat Germ Agglutinins
D002241:Carbohydrates; D007457:Iodine Radioisotopes; D037102:Lectins; D010835:Phytohemagglutinins; D037121:Plant Lectins; D011975:Receptors, Mitogen; C017707:Ricinus communis agglutinin-1; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C033619:lentil lectin; C028824:soybean lectin; D003208:Concanavalin A; D008358:Mannose; D005690:Galactose