The kinetic mechanism of acyl-CoA:lysolecithin acyltransferase from rabbit lung.
Acyl-CoA:lysolecithin acyltransferase is a key enzyme in the deacylation-reacylation pathway of biosynthesis of molecular species of lecithin. However, the mechanism of the reaction has been little studied. In this paper, the kinetic mechanism of acyl-CoA:lysolecithin acyltransferase, partially purified from rabbit lung, is studied. The double-reciprocal plots of initial velocity vs substrate concentration gave two sets of parallel lines which fitted to a ping-pong equation with the following parameters: Km (palmitoyl-CoA) = 8.5 +/- 2 microM, Km (lysolecithin) = 61 +/- 16 microM, and V = 18 +/- 4 nmol/min/mg protein. Inhibition studies by substrates, alternate substrates, and products supported the ping-pong mechanism, although some nonclassical behavior was observed. Palmitoyl-CoA did not inhibit even at concentrations of 100 Km. In contrast, lysolecithin was a dead-end inhibitor with a dissociation constant of Ki = 930 +/- 40 microM. Alternate substrates and CoA showed alternate pathways for the reaction due to the formation of ternary complexes. Dipalmitoylphosphatidylcholine inhibition pointed to an isomerization of the free enzyme prior to the start of the reaction. From these results, an iso-ping-pong kinetic mechanism for lysolecithin acyltransferase is proposed. The kinetic steps of the reaction are correlated with previous chemical studies of the enzyme.
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000032:analysis* / Q000037:antagonists & inhibitors; D000217:Acyltransferases / Q000032:analysis*; D000818:Animals; D003065:Coenzyme A / Q000378:metabolism; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D011487:Protein Conformation; D011817:Rabbits
D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D003065:Coenzyme A