Intensity and anisotropy decays of the Wye base of yeast tRNA(Phe) as measured by frequency-domain fluorometry.
The intensity and anisotropy decays of Wye base fluorescence from yeast tRNA(Phe) were determined by frequency-domain fluorometry. The intensity decay is at least a double exponential in the presence and absence of Mg2+, but the multi-exponential character of the decay is more pronounced in the absence of Mg2+. The anisotropy decay displays components due to overall tRNA rotational diffusion and to local torsional motions. The amplitude of the local motion is decreased 2-fold by the presence of Mg2+. The results are broadly consistent with a more homogeneous environment for the Wye base in the presence of Mg2+.
D002614:Chelating Agents; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D008274:Magnesium; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D015003:Yeasts / Q000032:analysis
D002614:Chelating Agents; D012346:RNA, Transfer, Amino Acyl; C012099:wye; D006147:Guanine; D008274:Magnesium