title: [Determination of phosphate residues participating in the formation of the spatial structure of tRNA- Leu IAG from cow mammary glands].
abstract: The phosphates of the tRNA-Leu IAG from cow mammary gland (tRNA which has a long variable loop) participating in the formation of three-dimensional structure were studied by alkylation with ethylnitrosourea and methylnitrosourea. A low degree of modification was observed for the phosphates of the following nucleotides: 7, 8, 9, 10 (at the bend site between the acceptor and D-stem); 18, 19, 20A and 21 in the D-loop; 47H and 49 at the joint of variable and T-stem; 57, 58 and 59 in the T-loop.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D008321:Mammary Glands, Animal / Q000032:analysis*; D009690:Nucleic Acid Conformation; D010710:Phosphates / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*
chemical_list: D010710:Phosphates; D012346:RNA, Transfer, Amino Acyl
vernacular_title: Opredelenie ostatkov fosfornoĭ kisloty, uchastvuiushchikh v obrazovanii prostranstvennoĭ struktury tRNK- Leu IAG molochnoĭ zhele korov.