A simple gas-flow chamber for cultured cell electrophysiology in a controlled atmosphere.
A simple and inexpensive device is described to control the gaseous environment while recording membrane potentials and contractile motion from single cultured cells. This equipment was used to study the electrophysiological and mechanical responses to hypoxia of cultured rat heart cells, but should also be suitable for a wide range of applications with several cell types.
D000200:Action Potentials; D000818:Animals; D001273:Atmosphere Exposure Chambers; D002462:Cell Membrane / Q000502:physiology*; D002478:Cells, Cultured; D004867:Equipment Design; D008564:Membrane Potentials; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology; D051381:Rats