On the potential of packed-column micro liquid chromatography with "in-column" fluorescence detection for trace analysis of drugs.
Packed fused-silica columns of e.g. 0.32 mm I.D. provide a form of chromatography (micro liquid chromatography) which can be surprisingly simple and highly sensitive. Existing commercial instrumentation with fluorescence detection "in column" (in the packing through the transparent column wall) leads to the determination of sub-picogram amounts of compounds in biological or other fluids. A detailed analysis is described as an example. Some aspects of micro liquid chromatography are discussed.
D002853:Chromatography, Liquid; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D004364:Pharmaceutical Preparations