[Demonstration of an NAD-dependent 6-phosphogluconate dehydrogenase in Pseudomonas syringae pv. phaseolicola].
Crude extracts from cells of Pseudomonas syringae pv. phaseolicola, a fluorescent pseudomonad, when grown on glucose contain a NAD-linked 6-phosphogluconate dehydrogenase. The reaction of the enzyme, which produces 14CO2 from 1-14C-6-phosphogluconate, is not inhibited by NaF, a potent inhibitor of the Enter-Doudoroff (ED) pathway enzyme 6-phosphogluconate dehydratase. In the presence of phosphate or arsenate ions the NAD-linked glyceraldehyde-3-phosphate dehydrogenase reacts with glyceraldehyde-3-phosphate which, in the ED pathway, is produced from 6-phosphogluconate and overlaps the 6-phosphogluconate dehydrogenase reaction. Only a small proportion of glucose is metabolized via the 6-phosphogluconate dehydrogenase/oxidative pentose phosphate pathway.
D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology
D009243:NAD; D012969:Sodium Fluoride; D010734:Phosphogluconate Dehydrogenase
Nachweis einer NAD abhängigen Gluconat-6-phosphat-Dehydrogenase in Pseudomonas syringae pv. phaseolicola.