Subcellular distribution of [3H]oestradiol-17 beta and salt resistance of nuclear oestrogen complexes in the rat uterus after in-vivo administration of [3H]oestradiol-17 beta.
The distribution of injected [3H]oestradiol-17 beta into nuclear and post-nuclear fractions as well as resistance to KCl (0.6 mol/l) extraction of nuclear oestrogen-binding sites in the rat uterus were studied. Both ovariectomized untreated and oestrogen-treated rats were used. One hour after injection of [3H]oestradiol into ovariectomized rats, 373 and 195 fmol/100 mg uterus were recovered in the nuclear and post-nuclear fractions respectively. About 17% of the nuclear-associated [3H]oestradiol was resistant to KCl extraction. After 18 h, the amount of [3H]oestradiol decreased to about 30% of that found after 1 h, and about 23% of nuclear complex remained resistant to extraction. In cardiac tissue, practically no [3H]oestradiol accumulated in the nuclear fractions and the amount recovered in post-nuclear fractions was about one-third of that in the uterine post-nuclear fractions. In the ovariectomized oestrogen-treated rats the distribution of [3H]oestradiol in nuclear and post-nuclear fractions was very similar and the proportion of the total nuclear fraction inextractable by KCl slightly higher (26%) than in untreated rats. After 18 h, while measurable amounts of [3H]oestradiol were found in post-nuclear fractions, no radioactivity was detected in the nuclear fraction. The results demonstrate the existence of salt-resistant nuclear oestrogen-binding sites in the mature ovariectomized rat uterus in vivo. Further, oestrogenization of ovariectomized rats did not significantly influence the amount or proportion of salt-resistant nuclear sites.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004958:Estradiol / Q000032:analysis / Q000378:metabolism*; D005260:Female; D009206:Myocardium / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D011189:Potassium Chloride; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000378:metabolism; D013347:Subcellular Fractions / Q000032:analysis; D014599:Uterus / Q000378:metabolism*
D011960:Receptors, Estrogen; D004958:Estradiol; D011189:Potassium Chloride