Evidence that the methylation inhibitor cycloleucine causes accumulation of a discrete ribosomal RNA precursor in hamster mitochondria.
A novel RNA fraction, 'Cy RNA,' that accumulates in mitochondria when hamster cells are treated with the methylation inhibitor cycloleucine, has been characterized by high resolution acrylamide gel electrophoresis and DNA-RNA hybridization. Cy RNA ran in gels as a discrete band, with an apparent chain length of 2 600. It hybridized specifically to restriction fragments containing genes for the mitochondrial ribosomal RNAs. We infer that Cy RNA is a discrete polycistronic ribosomal RNA precursor transcript, whose processing is dependent on normal methylation.
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003515:Cycloleucine / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D007668:Kidney; D008745:Methylation; D008928:Mitochondria / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009694:Nucleic Acid Precursors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012322:RNA Precursors; D012335:RNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
D000596:Amino Acids; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012335:RNA, Ribosomal; D003515:Cycloleucine