Analytical study of kallikrein and kallikrein-like esterase activity in subfractions from rat kidney cortex microsomes and isolated subcellular membranes.
Heavy and light microsomal fractions were subfractioned using high performance zonal rotors, and assayed for apoprotenin sensitive kallikrein-like amidolytic activity (pH 8.2). The activity profiles for the various substrates assayed show rather complex distribution pattern demonstrating kallikrein-like amidolytic activity in plasma membranes, basolateral membranes, rough endoplasmic reticulum and membranes derived from the Golgi complex.
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation; D007425:Intracellular Membranes / Q000201:enzymology*; D007610:Kallikreins / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D000626:Aminopeptidases; D007610:Kallikreins