Highly frequent detection of transforming genes in acute leukemias by transfection using in vivo selection assays.
DNAs from nine out of ten acute leukemia cases that were negative by in vitro focus forming assays exhibited transforming activity tested by in vivo selection assays in nude mice using transfected NIH3T3 cells. Of the nine cases, six cases contained activated N-ras genes, and one case exhibited activation of the c-K-ras gene. None of the ras gene family showed homology with the transforming genes derived from the other two cases. Our observations indicate that in vivo selection assays detect transforming genes including ras oncogenes at high frequency, and that activated N-ras genes are frequently detected in human acute leukemias.
D000818:Animals; D001681:Biological Assay; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009857:Oncogenes; D014162:Transfection
D004273:DNA, Neoplasm