Aqueous-organic solvent extraction of water-insoluble protein from ragweed pollen.
A water-insoluble material was obtained from either short ragweed (Ambrosia elatior) or false ragweed (Franseria acanthicarpa) pollen extracted by organic solvent (50% N,N-dimethylformamide). Further investigation of this water-insoluble material showed it contained at least five proteins. Two of them were isolated and shown to possess antigenicity as well as allergenicity. RFA2 contains carbohydrate and is a glycoprotein. RFB2 not only possesses antigenic determinants found in the water-soluble antigens AgE, AgK and Ra3, but also contains a separate and unique antigenic determinant. Immunodiffusion tests of RFB2, isolated from false ragweed and short ragweed, showed immunological identity. This communication further supports the suggestion that aqueous-organic solvent systems may be useful for the extraction of the biologically-active protein components from short ragweed pollen which can not be obtained with the currently used standard aqueous buffer system.
D000485:Allergens; D052179:Antigens, Plant; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification*; D011058:Pollen / Q000032:analysis* / Q000276:immunology; D011852:Radioallergosorbent Test; D012995:Solubility; D012997:Solvents; D014867:Water
D000485:Allergens; C026967:Amb a I protein, Ambrosia artemisiifolia; D052179:Antigens, Plant; D010940:Plant Proteins; D012997:Solvents; D014867:Water