Effect of ellipticine on MFO activity in single living 3T3 fibroblasts studied by microspectrofluorimetry.
The MFO activity in intact cells was monitored through microspectrofluorimetry study of BaP metabolization. This approach allowed observation of individual cells in a cell population to represent the studied samples through histograms as a function either of rate constants or of "residual BaP". The inhibitory effect of ellipticine was demonstrated on intact living 3T3 fibroblasts. The role of DMF dissolution and cellular localisation of compounds were also studied.
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism; D002460:Cell Line; D004126:Dimethylformamide / Q000494:pharmacology; D004611:Ellipticines / Q000494:pharmacology*; D005347:Fibroblasts / Q000201:enzymology; D051379:Mice; D006899:Mixed Function Oxygenases / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D000470:Alkaloids; D004611:Ellipticines; C034192:ellipticine; D001564:Benzo(a)pyrene; D004126:Dimethylformamide; D006899:Mixed Function Oxygenases