Age-specific nuclear proteins in the nematode worm Caenorhabditis elegans.
The nematode worm Caenorhabditis elegans is known to undergo characteristic morphological as well as physiological signs of senescence. Two-dimensional gel electrophoresis shows that alterations also occur in the pattern of the nuclear proteins as a function of age. Non-histone proteins whose level exhibits a steep fall with age are egg-specific and not involved in senescence. However, a distinct set of non-histones accumulates with age and can be considered as senescence markers. Some of these are glycoproteins, as shown by their concanavalin A-binding properties. One age-specific polypeptide, called 'protein S-28', was further characterized by peptide mapping and determination of its N-terminal amino acid sequence.
Meheus
L A
LA
Laboratorium voor Morfologie en Systematiek der Dieren, Rijksuniversiteit Gent, Belgium.
Van Beeumen
J J
JJ
Coomans
A V
AV
Vanfleteren
J R
JR
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D002107:Caenorhabditis / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism; D010449:Peptide Mapping; D010455:Peptides / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology*
D002868:Chromosomal Proteins, Non-Histone; D006023:Glycoproteins; D010455:Peptides; D013256:Steroids