NMR studies on an oligodeoxynucleotide containing 2-aminopurine opposite adenine.
A heteroduplex containing the mismatch 2-aminopurine (AP)-adenine has been synthesized and studied by proton NMR. The mismatch was incorporated into the sequence d[CGG(AP)GGC].d-(GCCACCG). One-dimensional nuclear Overhauser effect measurements in H2O and two-dimensional nuclear Overhauser effect spectra in D2O show AP.A base pairs in a wobble structure in which both bases are in the anti conformation. The adenine is stacked well in the helix, but the helix twist between the adenine and neighboring cytosine in the 3' direction is unusually small. As a result, the aminopurine on the opposite strand is somewhat pushed out of the helix. From the measurements of the imino proton line widths, the two adjacent G.C base pairs are not found to be significantly destabilized by the presence of the purine-purine wobble pair.
Fazakerley
G V
GV
Departement de Biologie, Centre d'Etudes Nucleaires de Saclay, Gif-sur-Yvette, France.
Sowers
L C
LC
Eritja
R
R
Kaplan
B E
BE
Goodman
M F
MF
D015075:2-Aminopurine; D000225:Adenine / Q000031:analogs & derivatives; D001482:Base Composition; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides
D009838:Oligodeoxyribonucleotides; D015075:2-Aminopurine; D000225:Adenine