Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector.
A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.
Ghosh-Choudhury
G
G
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Graham
F L
FL
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D006224:Cricetinae; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014162:Transfection
D004274:DNA, Recombinant; D013762:Tetrahydrofolate Dehydrogenase