Determination of metoprolol and two major metabolites in plasma and urine by column liquid chromatography and fluorometric detection.
Metoprolol and its alpha-hydroxy metabolite were determined in plasma down to 2 nmol/l (S.D. 10-15%) after solvent extraction and bonded-phase liquid chromatography with fluorometric detection. The major metabolite with a carboxylic function was also measured in plasma when liquid-solid extraction on a column activated with dodecyl sulphate was applied. In urine the three components were assayed by direct injection of a diluted sample.
Balmér
K
K
AB Hässle, Bioanalytical Chemistry, Mölndal, Sweden.
Zhang
Y Y
YY
Lagerström
P O
PO
Persson
B A
BA
D002853:Chromatography, Liquid; D006801:Humans; D007202:Indicators and Reagents; D008790:Metoprolol / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D008790:Metoprolol