Identification of nuclear envelope proteins and glycoproteins which co-isolate with the nuclear protein matrix.
Nuclear envelopes and nuclear matrices were isolated from rat liver nuclei. Although differences in polypeptide composition of the structures are evident on SDS gel electrophoresis, they have an almost identical distribution of concanavalin A-binding glycoproteins. These matrix-associated concanavalin A-binding glycoproteins derive entirely from the nuclear envelope and are recovered almost quantitatively in the matrix. They constitute easily identifiable markers for nuclear envelope association with matrix or other nuclear subfractions. Surface labelling of nuclei with 125I using solid-phase lactoperoxidase further confirmed that a large number of envelope-associated nuclear surface proteins co-isolate with the matrix. Protein kinase activity, as well as endogenous substrates for the kinase(s) are shown to be the same in both envelopes and matrix. Envelope-derived proteins and glycoproteins may comprise a substantial proportion of total matrix protein.
Smith
P J
PJ
UCT-CSIR Research Centre for Molecular Biology, Department of Biochemistry, University of Cape Town, Rondebosch, Republic of South Africa.
Sabbatini
G P
GP
Grant
K I
KI
von Holt
C
C
D000262:Adenylyl Cyclases / Q000032:analysis; D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D006023:Glycoproteins / Q000302:isolation & purification*; D006657:Histones / Q000302:isolation & purification; D008099:Liver / Q000032:analysis; D008565:Membrane Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000032:analysis* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000302:isolation & purification*; D051381:Rats
D006023:Glycoproteins; D006657:Histones; D008565:Membrane Proteins; D009687:Nuclear Proteins; D000262:Adenylyl Cyclases