title: Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group.
abstract: Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) was found to induce the synthesis of mRNA encoding a basic protein with a 67% amino acid sequence homology to the known acidic pathogenesis-related (PR) proteins 1a, 1b and 1c. By Southern blot hybridization it was shown that the tobacco genome contains at least eight genes for acidic PR-1 proteins and a similar number of genes encoding the basic homologues. Clones corresponding to three of the genes for acidic PR-1 proteins were isolated from a genomic library of Samsun NN tobacco. The nucleotide sequence of these genes and their flanking sequences were determined. One clone was found to correspond to the PR-1a gene; the two other clones do not correspond to known TMV-induced PR-1 mRNA's and may represent silent genes. Compared to the PR-1a gene, these genes contain an insertion or deletion in the putative promoter region and mutations affecting the PR-1 reading frame.
authors: Department of Biochemistry, Leiden University, The Netherlands.
authors: B J
authors: BJ
authors: Cornelissen
authors: J
authors: J
authors: Horowitz
authors: J A
authors: JA
authors: van Kan
authors: R B
authors: RB
authors: Goldberg
authors: J F
authors: JF
authors: Bol
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D014764:Viral Proteins