Efflux of 3H-thymidine by erythrocytes from mice infected with Trypanosoma brucei brucei.
Erythrocytes from mice infected with Trypanosoma brucei brucei showed a higher rate of efflux of labelled thymidine than did control erythrocytes from uninfected mice (0.56 +/- 0.10 and 0.38 +/- 0.06 mumole min-1 ml-1 packed cells respectively). Efflux of the nucleoside from erythrocytes of normal and infected mice were inhibited to the same extent by a specific nucleoside transport inhibitor, nitrobenzylthioinosine. Enumeration of nitrobenzylthioinosine binding sites on the erythrocytes showed that both have similar numbers of sites (6.2-6.6 X 10(3) sites/erythrocyte). It is concluded that the membrane permeability of the erythrocytes from infected mice was affected by the trypanosome in such a way as to enhance the purine nucleoside transport capacity. This may result in an increased supply of vital purine bases and nucleosides to trypanosomes which depend on their hosts for these nutrients.
Ogbunude
P O
PO
Nigerian Institute for Trypanosomiasis Research, Vom, Plateau State, Nigeria.
D000818:Animals; D001665:Binding Sites; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011687:Purines / Q000097:blood / Q000493:pharmacokinetics*; D013868:Thioinosine / Q000031:analogs & derivatives / Q000494:pharmacology; D013936:Thymidine; D014316:Tritium; D014346:Trypanosoma brucei brucei; D014353:Trypanosomiasis, African / Q000378:metabolism*
D011687:Purines; D014316:Tritium; D013868:Thioinosine; C001789:4-nitrobenzylthioinosine; D013936:Thymidine