Production of an ovine monoclonal antibody to testosterone by an interspecies fusion.
Hybridomas were made between NS1/1-Ag4-1 mouse myeloma cells and peripheral blood lymphocytes from a sheep immunized with a testosterone-protein conjugate. ELISAs were developed to detect ovine IgG and specific ovine anti-testosterone and these were used to screen the colonies. Although the cells fused acceptably only 3% of colonies secreted detectable quantities of antibody. The antibody was secreted at low levels (2.5 ng/ml) but was of high affinity (KD = 7.63 x 10(-12) M/1). One line, O/M 4.22, continued to secrete in culture for four months until the termination of the experiment.
Groves
D J
DJ
Department of Biochemistry, University of Surrey, Guildford, U.K.
Morris
B A
BA
Tan
K
K
De Silva
M
M
Clayton
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D002459:Cell Fusion; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D010954:Plasmacytoma / Q000276:immunology; D012756:Sheep; D013739:Testosterone / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D013739:Testosterone