[The small RNP acceptor of glucocorticoids bound to chromatin in liver cell nuclei].
The buoyant density in the CsCl gradient of the small nuclear RNP tightly bound to chromatin has been studied. It was shown that the buoyant density of alpha-RNP is characteristic for ribonucleoproteins (p = 1.36-1.50 g/cm3). The alpha-particles are of extraordinary stability. These RNP were shown to remain stable under drastic conditions (high ionic strength, SDS, 6 M urea) and resist unfixed caesium chloride density centrifugation. The alpha-RNA hybridizes with total rat liver DNA at C0t1/2 = 10(3). The oligonucleotide analysis of the alpha-RNA shows that the alpha-RNA is heterogeneous.
Konstantinova
I M
IM
Volkova
I V
IV
Kulichkova
V A
VA
Turoverova
L V
LV
Gauze
L N
LN
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000032:analysis; D007700:Kinetics; D008099:Liver / Q000032:analysis*; D008297:Male; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D012261:Ribonucleoproteins / Q000032:analysis*
D002843:Chromatin; D009841:Oligonucleotides; D011965:Receptors, Glucocorticoid; D012261:Ribonucleoproteins; D004247:DNA
Issledovanie malogo RNP-aktseptora gliukokortikoidov, sviazannogo s khromatinom iader kletok pecheni.