Role of membrane sialic acid content in the adhesiveness of aged erythrocytes to human cultured endothelial cells.
Following our previous observation that the oldest normal red blood cells were the most adherent to human cultured endothelial cells, we attempted to simulate this age-related adherence. Among all the membrane modifications experienced by erythrocytes during their life-span, loss of sialic acids has attracted considerable attention. Using two different preparations of neuraminidase, we performed a sialic acid depletion on the youngest erythrocytes to reach a sialic acid content similar to that observed in physiologically aged erythrocytes. These pretreated youngest cells displayed limited increase in the adhesiveness to endothelial cells, lower than that found with intact oldest cells. To obtain an adhesiveness of pretreated cells similar to that of naturally aged cells, it was necessary to exceed 80% of sialic acid depletion. At this extent of desialation, modifications of the electrophoretic pattern of glycophorins were observed as well as the appearance of peanut agglutinin reactivity which were never found in physiologically aged erythrocytes. Therefore, the sialic acid loss cannot be considered as being a single determinant factor of the naturally aged red cell adhesiveness.
Dhermy
D
D
INSERM U160, Hôpital Beaujon, Clichy, France.
Simeon
J
J
Wautier
M P
MP
Boivin
P
P
Wautier
J L
JL
D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004730:Endothelium, Vascular / Q000166:cytology*; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000502:physiology*; D004912:Erythrocytes / Q000166:cytology*; D005260:Female; D006384:Hemagglutination; D006801:Humans; D037102:Lectins; D019887:Peanut Agglutinin; D012156:Reticulocytes / Q000166:cytology; D012794:Sialic Acids / Q000097:blood*; D014471:Umbilical Veins
D037102:Lectins; D019887:Peanut Agglutinin; D012794:Sialic Acids