Stimulation of Paramecium phagocytosis by phorbol ester and forskolin.
Phorbol ester (PMA) exerted a dose- and time- dependent stimulating effect on phagocytosis in axenic Paramecium aurelia. When cells were exposed to 200-800 nM PMA in the presence of latex beads, the phagocytic coefficient was enhanced 2.25 to 3.14 times, during 10 min of continuous treatment and then rapidly declined. A similar effect was observed when the cells were exposed to a forskolin treatment, which resulted in nearly a twofold increase in phagocytic activity after a 10 min pulse. Both PMA and forskolin strongly stimulated phagocytosis (i.e. fivefold and threefold, respectively) in cells in which such activity had been completely inhibited by pre-exposure to the beta-receptor antagonist 1-propranolol.
Wyroba
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Department of Cell Biology, M. Nencki Institute of Experimental Biology, Warsaw, Poland.
D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D010247:Paramecium / Q000187:drug effects* / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects*; D010703:Phorbol Esters / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D013997:Time Factors
D010703:Phorbol Esters; D005576:Colforsin; D011433:Propranolol