Factors influencing the assay of Inoue-Melnick virus.
We describe factors influencing the assay of Inoue-Melnick virus (IMV) and practical comments for its assay. MRC-5 cells at a population doubling level (PDL) of more than 32 were more sensitive than younger cells for demonstrating cytopathic effect (CPE) of the virus. The PDL of MRC-5 cells also influenced the virus yield, particularly when the amount of viral inoculum was small. Cells at younger PDL produced less virus. The appearance of CPE and the virus yield were inhibited by increasing the concentration of NaHCO3 to 0.14% in the maintenance medium. The release of the virus into the medium was also inhibited as NaHCO3 was increased.
Nishibe
Y
Y
Institute for Virus Research, Kyoto University, Japan.
Inoue
Y K
YK
Melnick
J L
JL
D001639:Bicarbonates / Q000494:pharmacology; D001927:Brain Diseases / Q000382:microbiology*; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D014780:Viruses / Q000302:isolation & purification*
D001639:Bicarbonates; D003470:Culture Media