title: Intracellular K+, Na+ and Cl- concentrations and membrane potential in human monocytes.
abstract: The relationship between the resting membrane potential and the intracellular ionic concentrations in human monocytes was investigated. Cell volume, cell water content, and amount of intracellular K+, Na+, and Cl- were measured to determine the intracellular concentrations of K+ (Ki), Na+ (Nai) and Cl- (Cli) of monocytes, and of lymphocytes and neutrophils. Values found for monocytes were similar to those for neutrophils, i.e., cell volumes were 346 and 345 micron3, respectively, cell water content 78%, and Ki, 128 and 125, Nai, 24 and 26, and Cli, 102 and 103 mmol/l cell water, respectively. Lymphocytes, however, had different values: 181 micron3 cell volume, 77% cell water content, and for Ki, Nai, and Cli, 165, 37, and 91 mmol/l cell water, respectively. The resting membrane potential of cultured human monocytes (range -30 to -40 mV), determined by measurement of the peak potential occurring within the first milliseconds after microelectrode entry, was most dependent on extracellular K+, followed by Cl-, and Na+. The membrane permeability ratio of Cl- to K+ was estimated by use of the constant field equation to be 0.23 (range 0.22 to 0.30).
authors: Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
authors: C
authors: C
authors: Ince
authors: B
authors: B
authors: Thio
authors: B
authors: B
authors: van Duijn
authors: J T
authors: JT
authors: van Dissel
authors: D L
authors: DL
authors: Ypey
authors: P C
authors: PC
authors: Leijh
mesh_terms: D001826:Body Fluids / Q000032:analysis*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D006801:Humans; D007424:Intracellular Fluid / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D009994:Osmolar Concentration; D011188:Potassium / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D012964:Sodium; D011188:Potassium