title: [Glycosylated and nonglycosylated proteins of the tick-borne encephalitis virus synthesized in continuous pig embryonic kidney cells].
abstract: Synthesis of virus-specific proteins p93, p79, p69, p53, p47, p34, p24, p23, p21, p18, p15, p13, and p12 of which p53 and p13 are analogues of virion proteins V3 (E) and V2 (C) occurs in continuous pig embryo kidney (PEK) cells infected with tick-borne encephalitis virus. The third structural protein, p8 (V1, M) is found in virions but not in the cells. Treatment of the cells with cycloheximide and hypertonic NaCl solution, virtually depressing the radioactive label incorporation into PEK cell proteins, also inhibits the synthesis of virus-specific proteins p69, p21, p15, and p12. Protein p53 is present in the cells in glycosylated and nonglycosylated forms differing in electrophoretic mobility. Intensive glycosylation of not only p53 protein but also of proteins p47 and p21 was observed, and poor glycosylation of proteins p93, p79, and p69.
authors: V N
authors: VN
authors: Liapustin
authors: T S
authors: TS
authors: Gritsun
authors: V A
authors: VA
authors: Lashkevich
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004669:Encephalitis Viruses, Tick-Borne / Q000378:metabolism*; D004675:Encephalitis, Tick-Borne / Q000378:metabolism; D006031:Glycosylation; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D015678:Viral Structural Proteins; D014771:Virion / Q000378:metabolism; D014776:Virus Cultivation
chemical_list: D014764:Viral Proteins; D015678:Viral Structural Proteins
vernacular_title: Glikozilirovannye i neglikozilirovannye belki virusa kleshchevogo éntsefalita, sinteziruiushchiesia v perevivaemykh kletkakh pochki émbriona svin'i.