title: Study of cathepsin A, B and D activities in the skin wound edges. Its application to the differential diagnosis between vital and postmortem wounds.
abstract: The aims of the authors in this paper has been to check the diagnostic ability of the Cathepsin A, B and D concentrations in the skin wound edges to the differential diagnosis between vital and postmortem wounds. We have studied 56 domestic pigs grouped in seven experimental series consisting of 8 animals in each, according to the time (0, 5, 15, 30 min and 1, 3 and 6 h) after the injury. The enzymatic activities were investigated following the methods by Bowen and Davison Biochem. J., 131 (1973) 417-419, Suhar and Marks J. Biochem., 101 (1979) 23-30 and by Anson (modified by Yamamoto, Eur. J. Biochem., 95 (1979) 459-467) for Cathepsin A, B and D, respectively. For the differential diagnosis between vital and postmortem wounds, our results showed that the most useful markers studied are the Cathepsin A and D activities, Cathepsin D that of the first one.
authors: Cátedra de Medicina Legal, Facultad de Medicina, Universidad de Granada, Spain.
authors: C
authors: C
authors: Hernández-Cueto
authors: A
authors: A
authors: Luna
authors: J A
authors: JA
authors: Lorente
authors: E
authors: E
authors: Villanueva
mesh_terms: D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism*; D043402:Cathepsin A; D002401:Cathepsin B / Q000378:metabolism*; D002402:Cathepsin D / Q000378:metabolism*; D002403:Cathepsins / Q000378:metabolism*; D011180:Postmortem Changes; D012867:Skin / Q000293:injuries* / Q000473:pathology; D013552:Swine
chemical_list: D002268:Carboxypeptidases; D002403:Cathepsins; D043402:Cathepsin A; D002401:Cathepsin B; D002402:Cathepsin D