title: Interaction of spectrin with phospholipids. Quenching of spectrin intrinsic fluorescence by phospholipid suspensions.
abstract: Phospholipid suspensions prepared of phosphatidylethanolamine, phosphatidylserine and their mixtures are able to influence the intrinsic protein fluorescence of spectrin. In the case of phosphatidylethanolamine suspension up to 75% of protein fluorescence can be quenched. The interaction of phospholipid aggregates with spectrin is modulated by pH and ionic strength. Phospholipids, particularly phosphatidylethanolamine display a 'stabilizing' effect against the changes of protein fluorescence induced by increasing ionic strength and by thermal denaturation.
authors: Uniwersytet Wrocławski, Instytut Biochemii, Poland.
authors: A F
authors: AF
authors: Sikorski
authors: K
authors: K
authors: Michalak
authors: M
authors: M
authors: Bobrowska
mesh_terms: D004910:Erythrocyte Membrane / Q000032:analysis; D005227:Fatty Acids / Q000494:pharmacology; D005453:Fluorescence; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D010713:Phosphatidylcholines / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010718:Phosphatidylserines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D011487:Protein Conformation; D011489:Protein Denaturation; D013049:Spectrin; D013050:Spectrometry, Fluorescence
chemical_list: D005227:Fatty Acids; D046911:Macromolecular Substances; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; D013049:Spectrin