Typing of Aeromonas species by polyacrylamide-gel electrophoresis of radiolabelled cell proteins.
A method for typing species of Aeromonas by 35S-labelling and sodium-dodecyl sulphate polyacrylamide-gel electrophoresis (SDS-PAGE) of total soluble proteins is described. Radiolabelled-protein profiles from different strains were compared by use of the Dice coefficient. Typability was 100% and discrimination of pairs of differing strains was satisfactory with acceptable reproducibility. The results also yielded information on subgroups of phenotypic species.
Stephenson
J R
JR
Department of Medical Microbiology, St Bartholomew's Hospital Medical College, London.
Millership
S E
SE
Tabaqchali
S
S
D000333:Aeromonas / Q000032:analysis / Q000145:classification*; D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D006801:Humans; D014871:Water Microbiology
D001426:Bacterial Proteins