The chicken ovalbumin promoter is under negative control which is relieved by steroid hormones.
Steroid hormone regulation of activity of the chicken ovalbumin promoter was studied by microinjection of chimeric genes into the nuclei of primary cultured oviduct tubular gland cells. The chimeric genes contained increasing lengths of ovalbumin gene 5'-flanking sequences fused to the sequence coding for the SV40 T-antigen. Promoter activity was estimated by monitoring synthesis of T-antigen. The activity of the ovalbumin promoter is cell-specifically repressed in these oviduct cells and the repression is relieved upon addition of steroid hormones. The -132 to -425 region of the ovalbumin promoter which is responsible for this negative regulation behaves as an independent functional unit containing the regulatory elements necessary for both repression (in the presence of steroid hormone antagonists) and induced derepression (in the presence of steroid hormones) of linked heterologous promoters.
Gaub
M P
MP
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Dierich
A
A
Astinotti
D
D
Touitou
I
I
Chambon
P
P
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D002678:Chimera; D004274:DNA, Recombinant / Q000032:analysis; D004963:Estrenes / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D005938:Glucocorticoids / Q000037:antagonists & inhibitors; D015735:Mifepristone; D010047:Ovalbumin / Q000235:genetics*; D010057:Oviducts / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000187:drug effects; D013629:Tamoxifen / Q000494:pharmacology*
D004274:DNA, Recombinant; D004963:Estrenes; D005938:Glucocorticoids; D013629:Tamoxifen; D015735:Mifepristone; D011374:Progesterone; D010047:Ovalbumin