Detection of biotinylated DNA probes by using Eu-labeled streptavidin and time-resolved fluorometry.
Europium has been used as a label in immunoassays as it can be measured with high sensitivity by means of time-resolved fluorometry. Here we have used streptavidin labeled with europium chelates in the detection of adenovirus type 2 DNA bound to microtiter wells after hybridization with a biotinylated probe. The method gave quantitative results and a sensitivity of about 10 pg of the specific DNA.
Dahlén
P
P
Wallac Oy, Turku, Finland.
D000256:Adenoviridae / Q000235:genetics*; D001426:Bacterial Proteins; D001710:Biotin; D004279:DNA, Viral / Q000032:analysis*; D005063:Europium; D005470:Fluorometry; D009693:Nucleic Acid Hybridization; D019809:Streptavidin
D001426:Bacterial Proteins; D004279:DNA, Viral; D005063:Europium; D001710:Biotin; D019809:Streptavidin