Cytochemistry and biochemistry of acid phosphatases VII: Immunohistochemistry of canine prostatic acid phosphatase.
Acid phosphatase (E.C. 3.1.3.2.) has been isolated from canine prostatic gland homogenates by gel permeation chromatography (AcA34 or G150), by affinity chromatography (con A-Sepharose), or by using fluid phase liquid chromatography (FPLC) using Superose 12 and Mono P columns. Acid phosphatase-enriched fractions were submitted to analytical SDS-PAGE or to analytical isoelectric focusing. A protein with a molecular weight of 30 kD (on SDS gels) was used for immunization of rabbits. The antiserum produced was cross-reactive with prostatic acid phosphatase (canine and human) as shown by immunoblotting. When applied to paraffin or plastic sections of normal canine prostate, a positive immunoreaction was found exclusively in the secretory cells. In experimentally altered glands (castration and/or hormone treatment), a varying pattern of immunoreactive cells was found. In canine prostatic carcinomas, intensively reacting cell clusters were found along with nonreactive cells. The antiserum was also slightly cross-reactive with the respective human antigen, but the cross-reactivity of an antiserum prepared against human prostatic secretory acid phosphatase with canine prostatic acid phosphatase was far more pronounced.
Aumüller
G
G
Department of Anatomy and Cell Biology, Philipps-Universität, Marburg, Federal Republic of Germany.
Vedder
H
H
Enderle-Schmitt
U
U
Seitz
J
J
D000135:Acid Phosphatase / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D011467:Prostate / Q000201:enzymology* / Q000648:ultrastructure; D011471:Prostatic Neoplasms / Q000201:enzymology
D000941:Antigens; D000135:Acid Phosphatase