title: Structural proteins of bovine coronavirus and their intracellular processing.
abstract: The Quebec isolate of bovine coronavirus (BCV) was found to contain four unique major structural proteins. These proteins consisted of the peplomeric protein (gp190/E2, gp100/E2), the nucleocapsid protein (p53/N) and its apparent trimer (p160/N), a family of small matrix glycoproteins (gp26/E1, gp25/E1 and p23/E1) and the putative haemagglutinin (gp124/E3). Pulse-chase experiments utilizing polyclonal antiserum and monoclonal antibodies indicated that the unique BCV E3 protein had its primary precursor an N-linked glycoprotein with an Mr of 59,000 (gp59) which underwent rapid dimerization by disulphide bond formation to yield gp118. Further glycosylation of gp118 produced gp124/E3 which incorporated fucose. Thus gp124/E3 was probably a homodimer. The processing of the E2 and E1 proteins of BCV was similar to that shown previously for mouse hepatitis virus. A large N-linked precursor glycoprotein, gp170, underwent further glycosylation to yield gp190/E2 before subsequent proteolytic cleavage to yield gp100/E2. The glycosylated E1 (gp26, gp25) proteins arose as a result of O-linked glycosylation of p23/E1 as indicated by the resistance of these species to tunicamycin.
authors: University of Saskatchewan, Saskatoon, Canada.
authors: D
authors: D
authors: Deregt
authors: M
authors: M
authors: Sabara
authors: L A
authors: LA
authors: Babiuk
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid / Q000378:metabolism; D003332:Coronaviridae / Q000276:immunology / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D008970:Molecular Weight; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000032:analysis; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D014415:Tunicamycin / Q000494:pharmacology; D014758:Viral Core Proteins / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D006023:Glycoproteins; D010446:Peptide Fragments; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D014415:Tunicamycin