Gas chromatographic evaluation of trapidil and its desethyl metabolite in biological fluids for pharmacokinetic and bioavailability investigations.
This paper describes a suitable and cost-saving method for quantitative analysis of trapidil (5-methyl-7-diethylamino-s-triazolo [1,5-a]pyrimidine) and its desethyl metabolite in plasma and urine for pharmacokinetic and bioavailability investigations. Trapidil and desethyl-trapidil were extracted from plasma, concentrated and injected into the gas chromatography without any derivatization process. The authors suggest a series of internal standards. A thermoionic specific detector enables high sensitivity to be achieved, i.e. 20 ng/ml, and good specificity. The method is cost-saving in that it allows about 30 analyses a day to be performed without automatic integrators, and about 50 analyses a day with these integrators.
Marzo
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B.T.B. Industria Chimica S.p.A., Tribiano/Milano, Italy.
Pasotti
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V
Maggi
G C
GC
D001682:Biological Availability; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011743:Pyrimidines / Q000493:pharmacokinetics*; D014192:Trapidil / Q000031:analogs & derivatives / Q000032:analysis / Q000493:pharmacokinetics*
D011743:Pyrimidines; C054092:desdiethyltrapidil; D014192:Trapidil