Isolation, characterization, and pharmacological actions of peptide histidine valine 42, a novel prepro-vasoactive intestinal peptide-derived peptide.
The primary structure and biological activity of a novel prepro-vasoactive intestinal peptide (prepro-VIP)-derived peptide has been determined from an adrenal pheochromocytoma. The peptide was purified sufficiently for characterization by fast atom bombardment mapping after cation-exchange and reverse-phase fast protein liquid chromatography. The sequence of this novel peptide corresponds exactly to prepro-VIP-81-122 and has been designated peptide histidine valine 42 (PHV-42). Synthetic PHV-42 reduced both the force and frequency of spontaneous contractions of isolated rat uterus and was at least 12 times more potent than peptide histidine methionine (prepro-VIP-81-107), and over a hundred times more potent than noradrenaline. PHV-42 was also more potent than peptide histidine methionine in relaxing smooth muscle preparations of rat stomach and guinea pig trachea, but was approximately 4-fold less potent in reducing blood pressure than VIP. PHV-42 thus forms a separate subsystem in the VIP family of peptides and may be the most biologically active product of prepro-VIP in certain tissues such as the uterus and trachea.
Yiangou
Y
Y
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
Di Marzo
V
V
Spokes
R A
RA
Panico
M
M
Morris
H R
HR
Bloom
S R
SR
D000310:Adrenal Gland Neoplasms / Q000032:analysis*; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D010673:Pheochromocytoma / Q000032:analysis*; D011498:Protein Precursors / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects; D014660:Vasoactive Intestinal Peptide / Q000302:isolation & purification* / Q000494:pharmacology
D000596:Amino Acids; D010446:Peptide Fragments; D011498:Protein Precursors; C053900:peptide histidine valine 42; D014660:Vasoactive Intestinal Peptide