Evidence of a regulatory role of the level of poly (ADP-ribose) in chromosomal proteins in metallothionein gene expression by glucocorticoids but not by heavy metals.
The induction capacity of dexamethasone, a synthetic glucocorticoid, for the synthesis of metallothionein was about the same as that of 3-aminobenzamide, which is an inhibitor of ADP-ribosylation of chromosomal proteins, in cultured mouse mammary tumor cells. Both inductions of metallothionein were temporally correlated with a decrease in the amount of endogenous poly (ADP-ribose) on nonhistone high-mobility-group 14 and 17 proteins. In contrast, the extent of cadmium-induced metallothionein synthesis was 2-3-times that of dexamethasone or 3-aminobenzamide. However, cadmium had essentially no effect on de-ADP-ribosylation of these proteins.
Tanuma
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S
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Kawashima
K
K
Endo
H
H
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology*; D002460:Cell Line; D002875:Chromosomes / Q000187:drug effects / Q000502:physiology*; D003907:Dexamethasone / Q000494:pharmacology*; D005796:Genes / Q000187:drug effects*; D005809:Genes, Regulator; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D009687:Nuclear Proteins / Q000502:physiology*; D009702:Nucleoside Diphosphate Sugars / Q000502:physiology*; D011064:Poly Adenosine Diphosphate Ribose / Q000502:physiology*
D001549:Benzamides; D009687:Nuclear Proteins; D009702:Nucleoside Diphosphate Sugars; D002104:Cadmium; D011064:Poly Adenosine Diphosphate Ribose; D003907:Dexamethasone; C025160:3-aminobenzamide; D008668:Metallothionein