Isolation and characterization of a novel haemoprotein b-559 from Bengal gram (Cicer arietinum).
A haemoprotein was purified to apparent homogeneity from Bengal-gram seeds. The purified protein exhibited an absorption maximum at 412 nm (Soret band) that upon reduction with dithionite gave rise to a shift in the Soret band to 426 nm with concomitant appearance of an alpha-band at 559 nm and a beta-band at 530 nm. In the reduced state the Bengal-gram haemoprotein showed reactivity towards CO, nitrite and hydroxylamine. SDS/polyacrylamide-slab-gel electrophoresis showed that the haemoprotein has Mr 78,000. Gel-filtration and ultracentrifugal analyses suggest that the Bengal-gram haemoprotein is oligomeric in nature. Since it differs from photosynthetic membrane cytochrome b-559 in solubility in buffer, in reactivity towards CO and in molecular size, it appears to be a novel haemoprotein b-559.
Ramadoss
C S
CS
Department of Food Chemistry, Central Food Technological Research Institute, Mysore, India.
Shenoy
B C
BC
Borthakur
A
A
D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006418:Heme / Q000032:analysis; D006420:Hemeproteins / Q000302:isolation & purification*; D012639:Seeds / Q000032:analysis* / Q000201:enzymology; D013053:Spectrophotometry; D014461:Ultracentrifugation
D006420:Hemeproteins; C053803:hemeprotein b-559; D006418:Heme