Agrobacterium tumefaciens virulence locus pscA is related to the Rhizobium meliloti exoC locus.
Agrobacterium tumefaciens and Rhizobium meliloti carry related genetic loci which have important roles in virulence and symbiosis. Previously, it was shown that two virulence loci of A. tumefaciens, chvA and chvB, are related to two R. meliloti symbiosis loci, ndvA and ndvB, respectively (T. Dylan, L. Ielpi, S. Stanfield, L. Kashyap, C. Douglas, M. Yanofsky, E. Nester, D. R. Helinski, and G. Ditta, Proc. Natl. Acad. Sci. USA 83:4403-4407, 1986). Here we show that these two phytobacteria possess additional related virulence/symbiosis genes. Results of genetic complementation and DNA hybridization experiments indicate that the pscA virulence locus of A. tumefaciens is structurally and functionally related to the exoC symbiosis locus of R. meliloti. Thus, A. tumefaciens and R. meliloti bear at least three related genetic loci that have crucial roles in establishing the interactions that each bacterium has with its respective host plants.
Marks
J R
JR
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824.
Lynch
T J
TJ
Karlinsey
J E
JE
Thomashow
M F
MF
D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010944:Plants / Q000382:microbiology; D012231:Rhizobium / Q000235:genetics* / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D013559:Symbiosis
D004269:DNA, Bacterial