Type I and III collagen content and fibre distribution in normal human skin during ageing.
The content of type I and III collagen in normal human skin from subjects of different ages was studied by means of a new high performance liquid chromatography method and by SDS-polyacrylamide gel electrophoresis and scanning electron microscopy. The ratio of types I and III collagen in covered skin remained constant throughout childhood and young adult life and the proportion of type III was shown to be the same as previously reported. However, in the elderly, the proportion of type III collagen in the dermis increased to a variable degree. Scanning electron microscopic examination showed a decrease in the number of collagen fibre bundles with age. Average bundle width varied significantly with age. These results may reflect an impaired synthesis of type I collagen in aged skin.
Lovell
C R
CR
Department of Dermatology, Royal United Hospital, Bath, U.K.
Smolenski
K A
KA
Duance
V C
VC
Light
N D
ND
Young
S
S
Dyson
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000378:metabolism*; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D007223:Infant; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D012867:Skin / Q000378:metabolism* / Q000648:ultrastructure
D003094:Collagen; D006909:Hydroxyproline