Radioimaging of human ovarian carcinoma xenograft in nude mice.
Human ovarian carcinomas in nude mice were radioimaged using a well-characterized antibody against a tumor-associated antigen (CA 125) and three transplantable human ovarian carcinoma tumor lines: NIH:OVCAR 3, NIH:OVCAR 5, and NIH:OVCAR 9. Radioiodinated monoclonal antibody OC125 was used in these studies. In order to establish the optimal conditions for imaging, tumor/blood ratios were determined. Gamma scintigraphy of nude mice bearing subcutaneous transplants of human ovarian carcinomas 3-4 days after 131I-OC125 administration demonstrated selective localization of the radiolabeled monoclonal antibody by these tumors without need for any background subtraction techniques.
Manetta
A
A
Department of Obstetrics and Gynecology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Satyaswaroop
P G
PG
Hamilton
T
T
Ozols
R
R
Mortel
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D002460:Cell Line; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000032:analysis / Q000000981:diagnostic imaging*; D011877:Radionuclide Imaging; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes