Whole-body protein turnover in adult hemodialysis patients as measured by 13C-leucine.
Muscle wasting may occur in patients with chronic renal failure (CRF). To determine whether this is due to a decrease in the synthesis or an increase in the breakdown of muscle protein, we evaluated postabsorptive whole-body protein breakdown, oxidation, and synthesis rates at steady state during a primed, continuous infusion of 13C-leucine. This was done in seven subjects on chronic maintenance hemodialysis (MHD) and in seven normal control subjects. The protein breakdown rate in MHD was not different from that in controls (103 +/- 19 and 106 +/- 19 mumol leucine.kg-1.h-1, respectively). In MHD, however, the protein oxidation rate was 43% greater than that in controls (20 +/- 6 and 14 +/- 4 mumol leucine.kg-1.h-1, p less than 0.05), whereas net protein synthesis was less (p less than 0.05). Reduced net synthesis and increased oxidation rates of protein in the postabsorptive state may therefore contribute to the muscle-wasting syndrome in patients with CRF.
Berkelhammer
C H
CH
Department of Medicine, University of Toronto, Ontario, Canada.
Baker
J P
JP
Leiter
L A
LA
Uldall
P R
PR
Whittall
R
R
Slater
A
A
Wolman
S L
SL
D000328:Adult; D000368:Aged; D001774:Blood Chemical Analysis; D002153:Calorimetry, Indirect; D002247:Carbon Isotopes; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000378:metabolism / Q000628:therapy; D007930:Leucine / Q000493:pharmacokinetics; D008297:Male; D008875:Middle Aged; D009752:Nutritional Status; D011506:Proteins / Q000378:metabolism*; D006435:Renal Dialysis
D002247:Carbon Isotopes; D011506:Proteins; D007930:Leucine