Hemoglobin J-Baltimore (beta 16(A13)Gly----Asp): interference with the assay of HbA1c.
Three independent cases of Hemoglobin J-Baltimore(beta 16(A13)Gly----Asp) were detected through the assay of HbA1c in diabetic patients. Using chromatography on Bio-Rex 70 resin, one large peak replaced the usually well resolved peaks of HbA1a + b and HbA1c. The species that overlapped the latter fractions was identified as HbJ1c. HbJ-Baltimore itself was identified using HPLC of the beta-chain tryptic peptides. This observation emphasizes the errors that hemoglobin variants may introduce in the assay of HbA1c.
Vandenesch
F
F
CNRS UA 1171, Faculté de Médecine Grange-Blanche, Lyon, France.
Baklouti
F
F
Francina
A
A
Vianey-Liaud
C
C
Bertrand
A
A
Le Dévéhat
C
C
Delaunay
J
J
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood; D005189:False Positive Reactions; D006442:Glycated Hemoglobin / Q000032:analysis*; D006448:Hemoglobin J / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged
D006442:Glycated Hemoglobin; D006448:Hemoglobin J; D006455:Hemoglobins, Abnormal; C030639:hemoglobin J Baltimore