title: Changes in cell surface glycoprotein expression during differentiation of human keratinocytes.
abstract: Six cell surface glycoproteins defined by monoclonal antibodies were selected for study on human epidermal cells. In tests on tissue sections, three of the glycoproteins [J143 (gp140/30); T43 (gp85/36); H99 (gp38)] were expressed in the basal cell layer of the epidermis, whereas the other three glycoproteins [T179 (gp140/95); T16 (gp40/50); BT15 (gp80)] were preferentially expressed in maturing keratinocytes above the basal layer. We compared synthesis of these glycoproteins in fresh epidermis and in primary epidermal short term cultures using [35S]methionine for metabolic labeling. Synthesis of J143 was 8- to 20-fold higher and synthesis of T43 was 4- to 10-fold lower in cultured cells compared with fresh epidermis. BT15, an antigen strongly expressed on terminally differentiating keratinocytes, was synthesized at 5- to 15-fold higher levels in fresh epidermis than in cultured cells. Biosynthesis levels of H99, T179, and T16 did not change in cultured epidermal cells. Based on our findings, we propose a model of surface antigenic changes that occur during keratinocyte differentiation in vivo.
authors: Memorial Sloan-Kettering Cancer Center, New York, New York.
authors: C E
authors: CE
authors: Klein
authors: C
authors: C
authors: Cordon-Cardo
authors: R
authors: R
authors: Soehnchen
authors: R J
authors: RJ
authors: Cote
authors: H F
authors: HF
authors: Oettgen
authors: M
authors: M
authors: Eisinger
authors: L J
authors: LJ
authors: Old
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000096:biosynthesis* / Q000145:classification / Q000276:immunology; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000145:classification / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D008565:Membrane Proteins