Rapid micromethod for the analysis of mitotane and its metabolite in plasma by gas chromatography with electron-capture detection.
A rapid micromethod for 50-microliter samples is described for the analysis in plasma of mitotane (o,p'-DDD) and its metabolite (o,p'-DDE), using p,p'-DDD as internal standard. The compounds are extracted with heptane without sample pretreatment, and an aliquot is directly analysed by gas chromatography with electron-capture detection. Absolute recoveries for all three compounds were ca. 87% and coefficients of variation were less than 5%. The method is being used successfully for monitoring patients with Cushing's syndrome who receive chronic oral mitotane therapy.
Benecke
R
R
Section Biosciences, Karl Marx University, Leipzig, G.D.R.
Vetter
B
B
De Zeeuw
R A
RA
D002648:Child; D002849:Chromatography, Gas; D003480:Cushing Syndrome / Q000097:blood; D003633:Dichlorodiphenyl Dichloroethylene / Q000097:blood; D003632:Dichlorodiphenyldichloroethane / Q000097:blood; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D008939:Mitotane / Q000097:blood* / Q000493:pharmacokinetics
D007202:Indicators and Reagents; D003633:Dichlorodiphenyl Dichloroethylene; D008939:Mitotane; D003632:Dichlorodiphenyldichloroethane