Isolation of bovine cardiodilatin by fast protein liquid chromatography and reversed-phase high-performance liquid chromatography.
Cardiodilatin (CDD), a polypeptide exhibiting vasorelaxant and diuretic natriuretic bioactivity, was isolated from bovine atria. The isolation procedure reported here is different from that originally used for the purification of porcine and bovine CDD. Instead of cation-exchange chromatography on Fractogel TSK-CM 650 S and several purification steps on different high-performance liquid chromatographic (HPLC) columns, it is now possible to obtain CDD-88 by an automated fast-protein liquid chromatography system for repeated injections and a motor valve as fraction collector and only one final step of reversed-phase HPLC on a TSK-ODS-120T column.
Hock
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D
Institute of Anatomy III, University of Heidelberg, F.R.G.
Schriek
U
U
Fey
E
E
Forssmann
W G
WG
Mutt
V
V
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D009124:Muscle Proteins / Q000032:analysis / Q000302:isolation & purification*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D009124:Muscle Proteins; D009320:Atrial Natriuretic Factor; C040651:cardiodilatin