Equilibrium DNA-binding of AraC protein. Compensation for displaced ions.
Experiments on the AraC regulatory protein of Escherichia coli suggest a mechanism that DNA-binding proteins can use to reduce potentially drastic alterations in their affinity for DNA resulting from changes in salt concentration. Measurement of the net number of ions apparently displaced as AraC protein binds DNA and of fluorescence changes in the protein lead to the following picture. About 14 ions are displaced from the DNA as the protein binds the araI site. As the protein binds the DNA, however, it undergoes a conformational change and binds about ten ions. Consequently, the net order of the reaction is reduced from 15th to about fourth order in salt concentration.
Martin
K J
KJ
Department of Biochemistry, Brandeis University, Waltham, MA 02254.
Schleif
R F
RF
D051777:AraC Transcription Factor; D001426:Bacterial Proteins; D004247:DNA / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008954:Models, Biological; D011487:Protein Conformation; D012097:Repressor Proteins / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014157:Transcription Factors / Q000378:metabolism*
D051777:AraC Transcription Factor; C497371:AraC protein, E coli; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D004247:DNA