title: Antibody response to haptenic sugar antigen: immunodominancy of protein-bound lactose formed by amino-carbonyl reaction.
abstract: Several reducing sugars with structural analogy (glucose, galactose, lactose, melibiose, maltose and cellobiose) were bound to a carrier protein ovalbumin by amino-carbonyl reaction, and the non-enzymatically glycosylated proteins were injected into mice (BALB/c, C57BL/6, DBA/2 and C3H/He strains) with Freund's adjuvant. Antibody responses to the haptenic sugar antigens were analyzed quantitatively by enzyme-linked immunosorbent-assay using each sugar-bovine serum albumin complex. The haptenic sugar antigen from lactose induced a markedly higher response of specific antibody as compared with the haptenic sugar antigens from the other sugars. The antibody raised against the lactose adduct reacted well with lactulose (4-o-beta-D-galactopyranosyl-D-fructose) or alpha-N-acetyl-epsilon-N-deoxylactulosyl-L-lysine. The results suggested that immunogenicity of haptenic sugar antigens was remarkably different in sugar chemical structure, and that the lactose adduct formed by lactose-protein amino-carbonyl reaction could be an immunodominant antigenic determinant.
authors: Department of Food Science and Technology, School of Agriculture, Nagoya University, Japan.
authors: T
authors: T
authors: Matsuda
authors: I
authors: I
authors: Nakashima
authors: Y
authors: Y
authors: Kato
authors: R
authors: R
authors: Nakamura
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002241:Carbohydrates / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006031:Glycosylation; D006241:Haptens / Q000276:immunology*; D007785:Lactose / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D006241:Haptens; D010047:Ovalbumin; D007785:Lactose