The adenovirus major late transcription factor activates the rat gamma-fibrinogen promoter.
The major late transcription factor (MLTF) is a 46-kilodalton polypeptide that specifically binds to and activates transcription from the major late promoter of adenovirus. The presence of this promoter-specific transcription factor in uninfected HeLa cell extracts suggests that MLTF is also involved in the transcription of cellular genes. This report demonstrates that MLTF specifically stimulates transcription of the rat gamma-fibrinogen gene through a high-affinity binding site. Stimulation of transcription by MLTF was not dependent on the exact position of the MLTF binding site with respect either to the transcription initiation site or to adjacent promoter elements. These results suggest that one of the cellular functions of MLTF is to control gamma-fibrinogen gene expression.
Chodosh
L A
LA
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Carthew
R W
RW
Morgan
J G
JG
Crabtree
G R
GR
Sharp
P A
PA
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D005340:Fibrinogen / Q000235:genetics*; D005786:Gene Expression Regulation; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D005340:Fibrinogen; D012319:RNA Polymerase II