title: Gabaergic neurons of the hippocampus: development in homotopic grafts and in dissociated cell cultures.
abstract: The hippocampus taken from E18-E19 rat embryos was dissociated into a cell suspension and was either grafted into the hippocampus of adult rats or cultured. The growth of GABAergic neurons was examined using a GABA directed antiserum. The implanted tissue was capable of survival and growth without exhibiting a laminar organization. Most of the various morphological neuronal types could be observed, establishing different types of synapses; however, granule neurons were rarely encountered. A substantial proportion of GABA-positive neurons was detected within the graft with profuse labelling of the neuropil. In cultures issued from the same cell suspension, GABA-immunoreactive neurons were numerous and had different morphologies. Altogether these data suggest that GABA neurons express a high potential for growth and sprouting in vitro and in vivo.
authors: INSERM U-29, Höpital de Port-Royal, Paris, France.
authors: O
authors: O
authors: Robain
authors: G
authors: G
authors: Barbin
authors: Y
authors: Y
authors: Ben-Ari
authors: F
authors: F
authors: Rozenberg
authors: A
authors: A
authors: Prochiantz
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D006085:Graft Survival; D006624:Hippocampus / Q000166:cytology* / Q000196:embryology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D005680:gamma-Aminobutyric Acid