Minor components of Hb Bart's.
The minor components of Hb Bart's were separated by CM-cellulose chromatography, reverse-phase HPLC, and DEAE-cellulose chromatography. These were characterized by amino acid analysis, tryptic peptide analysis by HPLC, electrophoresis, and carbohydrate and phosphate analysis. Acetylated and glycated components of Hb Bart's were present in cord blood red cells. The ratio of gamma Ic/total gamma in Hb Bart's was nearly the same as that of Hb FIc/Hb F which may indicate that the gamma chain after its release from the ribosome is not a better acetylation substrate than Hb Fo. Glycation of the free gamma-chains seems to take place in vivo as readily as the major Hb components.
Abraham
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Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-3331.
Abraham
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Kasten-Jolly
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D002266:Carboxymethylcellulose Sodium; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D005312:Fetal Blood / Q000032:analysis; D005914:Globins / Q000302:isolation & purification; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D013789:Thalassemia / Q000097:blood
D006455:Hemoglobins, Abnormal; D005914:Globins; C030448:hemoglobin Bart's; D002266:Carboxymethylcellulose Sodium