title: Roles of neonatal and prepubertal testicular androgens on androgen-induced proliferative response of seminal vesicle cells in adult mice.
abstract: Male mice were castrated at 0, 10, 20, 30, 40 and 60 days of age; daily injections of testosterone propionate (TP, 4 micrograms/g b. wt) were started from day 90. On various days after starting the TP injections, the incorporation of 5-[125I]iodo-2'-deoxyuridine into the whole seminal vesicles was determined as an index for proliferation. The seminal vesicle cells in mice castrated on days 0 and 20 were characterized by low weight (0.5-1 mg) before TP injection, long duration of androgen-induced proliferation (greater than 20 days) with a low peak, and involvement of both epithelial and fibromuscular cells (neonatal castration type). The seminal vesicle cells in mice castrated on days 60 and 40 were characterized by relatively high weight (5-10 mg) before TP injection, short duration of androgen-induced proliferation (10 days) with a high peak, and involvement of only the epithelial cells (adult castration type). In mice castrated on days 0 and 20, the neonatal castration type of androgen-induced proliferation was completely changed to the adult castration type when TP pretreatment (2 micrograms/g b. wt per 12 h) had been given from day 20 to day 40. However, the TP pretreatment given from day 90 to day 110 instead of days 20-40 had no such effect in 140-day old mice castrated on day 0. The present findings suggest that testicular androgens secreted from day 20 to day 40 play an indispensable role in the induction of irreversible proliferative response of the mouse seminal vesicle. The activity of the prepubertal androgens may not be completely compensated by androgen activity at adulthood.
authors: Department of Pathology, Osaka University Medical School, Japan.
authors: T
authors: T
authors: Yamane
authors: M
authors: M
authors: Ayata
authors: S
authors: S
authors: Okamoto
authors: N
authors: N
authors: Terada
authors: Y
authors: Y
authors: Kitamura
authors: K
authors: K
authors: Matsumoto
mesh_terms: D000728:Androgens / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D007065:Idoxuridine / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012669:Seminal Vesicles / Q000166:cytology*; D012741:Sexual Maturation; D013737:Testis / Q000254:growth & development*; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D000728:Androgens; D013739:Testosterone; D007065:Idoxuridine