Structure of sialyl-oligosaccharides isolated from bronchial mucus glycoproteins of patients (blood group O) suffering from cystic fibrosis.
The carbohydrate chains of the bronchial-mucus glycoproteins of six cystic fibrosis patients with blood group O were released by alkaline borohydride treatment. Low-molecular-mass, monosialyl oligosaccharide-alditols were isolated by anion-exchange chromatography and fractionated by high-performance liquid chromatography. Structural characterization was performed by 500-MHz 1H-NMR spectroscopy in combination with quantitative sugar analysis. The established structures range in size from tetra- up to heptasaccharides. They are all sialyl analogs of neutral oligosaccharides that were characterized previously [Lamblin G., Boersma A., Lhermitte M., Roussel P., Mutsaers J. H. G. M., Van Halbeek H. & Vliegenthart J. F. G. (1984) Eur. J. Biochem. 143, 227-236]. The NeuAc residue was found to occur either in alpha (2----3)-linkage to Gal, or in alpha (2----6)-linkage to GalNAc-ol or Gal.
Breg
J
J
Department of Bio-organic Chemistry, University of Utrecht.
Van Halbeek
H
H
Vliegenthart
J F
JF
Lamblin
G
G
Houvenaghel
M C
MC
Roussel
P
P
D000017:ABO Blood-Group System; D001980:Bronchi / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003550:Cystic Fibrosis / Q000378:metabolism*; D004735:Energy Transfer; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D000017:ABO Blood-Group System; D006023:Glycoproteins; D009844:Oligosaccharides; C047874:gastric mucus glycoproteins; C016735:sialooligosaccharides