title: Polyamine metabolism in Acanthamoeba: polyamine content and synthesis of ornithine, putrescine, and diaminopropane.
abstract: Five polyamines which could be separated by high performance liquid chromatography were found in Acanthamoeba castellanii (strain Neff). These included in order of decreasing abundance: 1,3-diaminopropane, spermidine, spermine, norspermidine, and putrescine. Only diaminopropane and norspermidine had been found previously. Spermine was present in cultures grown in broth, but not in defined medium. Radioactive substrates were used to establish that putrescine was synthesized by decarboxylation of ornithine, ornithine was synthesized from arginine or citrulline, and diaminopropane was synthesized from spermidine. The presence of ornithine decarboxylase (EC 4.1.1.17), arginase (EC 3.5.3.1), and urease (EC 3.5.1.5) and the absence of arginine decarboxylase (EC 4.1.1.19) were established. A scheme for polyamine biosynthesis in A. castellanii is proposed.
authors: Department of Microbiology, Ohio State University, Columbus 43210.
authors: B G
authors: BG
authors: Kim
authors: A
authors: A
authors: Sobota
authors: A J
authors: AJ
authors: Bitonti
authors: P P
authors: PP
authors: McCann
authors: T J
authors: TJ
authors: Byers
mesh_terms: D000048:Acanthamoeba / Q000378:metabolism*; D000818:Animals; D001119:Arginase / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D002956:Citrulline / Q000378:metabolism; D003959:Diamines / Q000378:metabolism*; D009952:Ornithine / Q000096:biosynthesis*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011073:Polyamines / Q000032:analysis / Q000378:metabolism*; D011700:Putrescine / Q000096:biosynthesis*; D013095:Spermidine / Q000031:analogs & derivatives / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D014510:Urease / Q000378:metabolism
chemical_list: D003959:Diamines; D011073:Polyamines; D002956:Citrulline; D013096:Spermine; C006760:norspermidine; D001120:Arginine; C009475:trimethylenediamine; D009952:Ornithine; D014510:Urease; D001119:Arginase; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine