Tubulin sequence region beta 155-174 is involved in binding exchangeable guanosine triphosphate.
Assembly-competent microtubule protein was directly photoaffinity labeled with [alpha-32P]guanosine triphosphate by UV irradiation. The labeled tubulin was digested with trypsin. The radioactive fragments were isolated and sequenced, revealing beta-tubulin residues 155-174 to be the major labeled region. An antibody to a synthetic peptide comprising residues beta 154-165 inhibits GTP incorporation and tubulin polymerization.
Hesse
J
J
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.
Thierauf
M
M
Ponstingl
H
H
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D006160:Guanosine Triphosphate / Q000378:metabolism*; D010777:Photochemistry; D011108:Polymers / Q000378:metabolism; D013552:Swine; D014357:Trypsin / Q000378:metabolism; D014404:Tubulin / Q000032:analysis*; D014466:Ultraviolet Rays
D000345:Affinity Labels; D011108:Polymers; D014404:Tubulin; D006160:Guanosine Triphosphate; D014357:Trypsin