Rapid isolation and identification of reovirus-3.
Mammalian reoviruses are connected with a variety of humans diseases, including gastroenteritis, malabsorption and hepatitis. Recently, reovirus-3 was found to be associated with neonatal biliary atresia. We describe a technique for the rapid isolation and identification of reovirus-3. Mouse fibroblasts (L 929 cells) were grown in monolayers in a RPMI 1640 medium containing 10% calf serum. The cytopathic effects were visualized by the rounding of the L 929 cells and the appearance of fine granulation in the cytoplasm 48 h after the infection. Hematoxylin-eosin staining showed swelling and rounding of the infected cells, diminished chromatin in the nuclei, and the absence of mitoses. The immunohistochemical staining by the avidin-biotin-peroxidase technique was positive in the infected monolayers of the L 929 cells. The positive staining was limited to cytoplasmic inclusions, which were surrounded by a halo and sometimes by vacuoles. We conclude that the described technique is useful for the rapid isolation and identification of reovirus-3.
Abramowitz
A
A
Department of Pediatrics, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Arad
G
G
Livni
N
N
Morag
A
A
Tamir
I
I
D000818:Animals; D001360:Avidin; D001710:Biotin; D007150:Immunohistochemistry; D012089:Mammalian orthoreovirus 3 / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D051379:Mice; D012087:Reoviridae / Q000302:isolation & purification*
D001360:Avidin; D001710:Biotin