Dietary fat regulation of the association of protein kinase C activity with epidermal cell membranes.
An apparent redistribution of protein kinase C activity was noted in the particulate and soluble fractions of basal epidermal cells isolated from mice receiving semi-purified diets varying in fat content. An increase in the amount of protein kinase C activity in the particulate fraction and a decrease in the soluble fraction were observed with the mice fed high-fat (24.6% corn oil) diet as compared with those given the low-fat (5% corn oil) diet. Increased levels of phospholipids and an increase in 18:2 and 18:3 side chains of the phospholipids were observed with the high-fat diet.
Donnelly
T E
TE
Department of Pharmacology, Univeristy of Nebraska Medical Center, Omaha 68105.
Birt
D F
DF
Sittler
R
R
Anderson
C L
CL
Choe
M
M
Julius
A
A
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004817:Epidermis / Q000201:enzymology*; D008297:Male; D051379:Mice; D010743:Phospholipids / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*
D004041:Dietary Fats; D010743:Phospholipids; D011493:Protein Kinase C