title: [Nuclear proteins--substrates for cAMP-dependent protein kinase].
abstract: The phosphorylation of nuclear proteins of porcine brain cAMP-dependent protein kinase was studied. Some nuclear proteins after extraction from the nuclei served as substrates for protein kinase. Lysine-rich histones H1, H2a and H2b were found to accept phosphate during chromatin phosphorylation by cAMP-dependent protein kinase. Phosphorylation of intact nuclei revealed that in such a system only histone H1 is a substrate for cAMP-dependent protein kinase. In the presence of DNA the histones are phosphorylated by cAMP-dependent protein kinase in a different manner. It was concluded that DNA can determine the accessibility of protein substrates for the catalytic subunit of cAMP-dependent protein kinase.
authors: M V
authors: MV
authors: Nesterova
authors: A I
authors: AI
authors: Glukhov
authors: A G
authors: AG
authors: Aprikian
authors: E S
authors: ES
authors: Severin
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine
chemical_list: D006657:Histones; D011494:Protein Kinases
vernacular_title: Iadernye belki--substraty cAMP-zavisimoĭ proteinkinazy.