Inactivation of bladder tumor cells and enzymes by methylene blue plus light.
Using a cystoscopic light source and methylene blue as the sensitizing dye, photoactivation was examined in two types of experiments. In the first, the in vitro study destruction of two enzymes (glucose-6-phosphate dehydrogenase and lactic dehydrogenase) was examined in suspensions of whole and homogenized tumor cells from a transplantable bladder tumor. In the second or in vivo study rats were used to demonstrate that tumor cell suspensions treated with methylene blue plus light, when inoculated into susceptible rats, failed to "take" and produce new tumors. These experiments suggest a possible therapeutic use in treatment of human bladder tumors, though further study would be required.
Gill
W B
WB
Department of Surgery (Urology), University of Chicago, Illinois 60637.
Taja
A
A
Chadbourne
D M
DM
Roma
M
M
Vermeulen
C W
CW
D000818:Animals; D004353:Drug Evaluation, Preclinical; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000528:radiation effects; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors / Q000528:radiation effects; D008751:Methylene Blue / Q000627:therapeutic use*; D009368:Neoplasm Transplantation; D010778:Photochemotherapy / Q000379:methods*; D011838:Radiation-Sensitizing Agents; D051381:Rats; D001743:Urinary Bladder / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000201:enzymology
D011838:Radiation-Sensitizing Agents; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D008751:Methylene Blue