Possible involvement of protein kinase C (PKC) in the regulation of electrically evoked serotonin (5-HT) release from rabbit hippocampal slices.
Protein kinase C (PKC)-activating phorbol esters enhanced the electrically evoked 5-HT release from rabbit hippocampal slices preincubated with [3H]5-HT. The release was diminished by polymyxin B, an inhibitor of PKC. These results are compatible with a stimulatory effect of PKC on the 5-HT release induced by action potentials. The mutual effects of PKC affecting drugs on 5-HT release suggest a functional but not a competitive interaction. The attenuation or the enhancement of effects of 5-HT autoreceptor ligands at various 5-HT biophase concentrations found after PKC-affecting drugs are in line with the view that autoreceptor-mediated events are not directly influenced by the enzyme PKC.
Feuerstein
T J
TJ
Institut für Pharmakologie und Toxikologie, Universität Freiburg, F.R.G.
Allgaier
C
C
Hertting
G
G
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D004347:Drug Interactions; D004558:Electric Stimulation; D005089:Exocytosis / Q000187:drug effects; D006624:Hippocampus / Q000378:metabolism* / Q000502:physiology; D066298:In Vitro Techniques; D009411:Nerve Endings / Q000187:drug effects / Q000378:metabolism; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism*
D010703:Phorbol Esters; D012701:Serotonin; D011493:Protein Kinase C