Direct selection of a specifically blocked mutant of Actinomadura brunnea. Isolation of a third 8-methoxy substituted chlortetracycline.
Actinomadura brunnea produces 2'-N-methyl-8-methoxychlortetracycline. A derivative of this strain has been isolated that is specifically blocked in methylation of the 2'-amino position. This isolate was detected by screening approximately 30,000 colonies of a mutagenized population of Actinomadura brunnea using a direct soft agar overlay with an Escherichia coli indicator. The antibiotic produced by the blocked mutant has been identified as 8-methoxychlortetracycline (Sch 36969) based upon its biological activity, relative mobility on TLC and HPLC, and spectroscopic data.
Smith
E B
EB
Microbiological Sciences, Schering Corporation, Bloomfield, NJ 07003.
Munayyer
H K
HK
Ryan
M J
MJ
Mayles
B A
BA
Hegde
V R
VR
Miller
G H
GH
D055598:Chemical Phenomena; D002621:Chemistry; D002751:Chlortetracycline / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D009154:Mutation; D009618:Nocardiaceae / Q000378:metabolism*; D013754:Tetracyclines / Q000302:isolation & purification* / Q000494:pharmacology
D013754:Tetracyclines; C054355:8-methoxychlortetracycline; D002751:Chlortetracycline