The effect of L-cysteine on the portion-selective uptake of cadmium in the renal proximal tubule.
Cadmium (Cd), co-administered with an excess of L-cysteine, accumulates rapidly in the kidneys of the rat. After subcutaneous (s.c.) injection of 3 mumol CdCl2/kg body wt the concentrations of Cd in the blood and kidneys increase with the dose of cysteine over the range 0.06-5.0 mmol/kg body wt. At cysteine doses of less than 1.5 mmol/kg body wt the ratio of the concentrations of Cd in the outer medulla and cortex of the kidney remains the same as that after the injection of Cd alone. This ratio, however, is more than doubled at dose levels of 5-10 mmol cysteine/kg body wt. Hepatic uptake of Cd is unaffected by doses of cysteine below 1.5 mmol/kg body wt but decreases markedly at higher doses. In animals that are dosed simultaneously with 5 mmol cysteine/kg body wt, renal uptake of 109Cd is known to occur in the straight segments of the proximal tubules. At a dose level of less than 1.5 mmol cysteine/kg body wt the present autoradiographical studies show that 109Cd is taken up predominantly by the proximal convoluted tubules of the kidney cortex. At the critical dose level (1.5 mmol/kg body wt), cysteine decreases the retention of Cd at the s.c. injection site, but probably has little effect on the distribution of Cd between protein and other carrier molecules in the blood. This distribution, however, is altered at higher cysteine dose levels. It is suggested that, under the latter conditions, stable Cd-cysteine complexes are formed in the blood and are filtered readily through the glomeruli.(ABSTRACT TRUNCATED AT 250 WORDS)
Murakami
M
M
Basic Medical Sciences Division, National Institute for Environmental Studies, Japan Environment Agency, Ibaraki.
Sano
K
K
Webb
M
M
D000818:Animals; D001345:Autoradiography; D002104:Cadmium / Q000493:pharmacokinetics*; D002106:Cadmium Radioisotopes; D003545:Cysteine / Q000493:pharmacokinetics / Q000494:pharmacology*; D005260:Female; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013462:Sulfur Radioisotopes; D014018:Tissue Distribution
D002106:Cadmium Radioisotopes; D013462:Sulfur Radioisotopes; D002104:Cadmium; D003545:Cysteine