Effects of methylglyoxal bis(guanylhydrazone) and two phenylated analogues on S-adenosylmethionine decarboxylase activity from Eimeria stiedai (Apicomplexa).
1. Activity of S-adenosylmethionine decarboxylase, one of the rate-limiting enzymes of polyamine biosynthesis, was determined in oocysts of Eimeria stiedai, a coccidian parasite of the rabbit. 2. Several properties of the enzyme were compared to the mammalian enzyme. It showed considerably less substrate affinity than the analog enzyme from the rabbit. 3. The E. stiedai enzyme showed a low sensitivity to methylglyoxal bis(guanylhydrazone), a frequently used inhibitor of the enzyme in mammals, and two phenylated derivatives. 4. Results with the inhibitors are discussed in view of their potential use in chemotherapy.
San-Martín Núñez
B
B
Departamento de Toxicología y Farmacología, Facultad de Veterinaria Universidad Complutense de Madrid, Spain.
Alunda
J M
JM
Balaña-Fouce
R
R
Ordóñez Escudero
D
D
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism*; D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D004539:Eimeria / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008935:Mitoguazone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology
C048255:propylglyoxal bis(guanylhydrazone); C049009:diphenylglyoxal bis(guanylhydrazone); D002262:Carboxy-Lyases; D012437:Adenosylmethionine Decarboxylase; D008935:Mitoguazone