[An antioxidant prevents and reverses calcium-induced uncoupling of rat liver mitochondria].
Accumulation of Ca2+ by rat liver mitochondria in the presence of inorganic phosphate results in spontaneous activation of respiration accompanied by a progressive loss of the accumulated cation. The lipid peroxidation inhibitor, ionol, completely prevents and reverses the Ca2+/phosphate-induced loss of accumulated Ca2+ and restores the respiration to state 4 level without having any effect on the rate of Ca2+ accumulation and respiration in the presence of an uncoupler. No correlation between the ionol-dependent loss of Ca2+ and the formation of malonic dialdehyde in mitochondria was found. The measurements of delta psi across the inner mitochondrial membrane during a progressive loss of Ca2+ suggest that the Ca2+/phosphate-induced "uncoupling" is mainly due to the appearance of electrogenic fluxes (but not Ca2+ cycling) which is under control of some products of initial steps of lipid peroxidation.
Novgorodov
S A
SA
Gogvadze
V G
VG
Medvedev
B I
BI
Zinchenko
V P
VP
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010710:Phosphates / Q000494:pharmacology; D051381:Rats; D014475:Uncoupling Agents / Q000037:antagonists & inhibitors*
D000975:Antioxidants; D010710:Phosphates; D014475:Uncoupling Agents; D002084:Butylated Hydroxytoluene; D002118:Calcium
Antioksidant predotvrashchaet i obrashchaet indutsiruemoe ionami kal'tsiia razobshchenie mitokhondriĭ pecheni krys.