title: The prognostic significance of proliferative activity in poor histology non-Hodgkin's lymphoma: a flow cytometry study using archival material.
abstract: The DNA content and proliferative activity of paraffin-embedded lymph node tissue from 111 patients with poor histology non-Hodgkin's lymphoma were measured by flow cytometry. These patients had been entered into a prospective randomized trial which, to date, has shown no survival difference by treatment arm. Forty-four (40%) samples showed evidence of aneuploidy with three samples having more than one aneuploid population. The aneuploid populations had a bimodal distribution with one group having a DNA index between 1.1-1.3 and the other 1.8-2.2. The incidence of aneuploidy did not correlate with age, stage or survival. In 56 diploid samples the S phase values below 10% had a significantly better survival than those with S phase values above 10% (P less than 0.011). For patients with diffuse large cell histology the corresponding discriminatory S phase value was 19% (P less than 0.009).
authors: Kanematsu Laboratories, Royal Prince Alfred Hospital, Camperdown, Sydney, Australia.
authors: G A
authors: GA
authors: Young
authors: D W
authors: DW
authors: Hedley
authors: C A
authors: CA
authors: Rugg
authors: H J
authors: HJ
authors: Iland
mesh_terms: D000328:Adult; D001315:Australia; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis
chemical_list: D004273:DNA, Neoplasm