Measurement of the rate of substrate cycling between acetate and acetyl-CoA in sheep muscle in vivo. Effects of infusion of acetate.
1. The rate of substrate cycling between acetate and acetyl-CoA in sheep hindlimb muscle was measured from the decrease of [14C]acetate specific radioactivity across the tissue in vivo. 2. The rate of cycling was 0.005-0.015 mumol/min per g and, in two of the three sheep investigated, was approximately doubled as a result of infusing 2 mol acetate/day intravenously. 3. The results indicate that this cycle contributes only approx. 0.5% of the total heat produced by the animal and would probably make only a relatively small contribution to any heat increment of feeding associated with diets producing large amounts of acetate.
Crabtree
B
B
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, U.K.
Marr
S A
SA
Anderson
S E
SE
MacRae
J C
JC
D000085:Acetates / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology; D019342:Acetic Acid; D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D001833:Body Temperature Regulation; D002952:Citric Acid Cycle; D008297:Male; D009132:Muscles / Q000378:metabolism*; D012756:Sheep
D000085:Acetates; D000105:Acetyl Coenzyme A; D019342:Acetic Acid