The rapid and sensitive enumeration of antibody-secreting cells using immunogold/silver staining (SIG-blot assay).
A simple and robust method has been developed for the enumeration of antibody-secreting cells. Secretion is assessed on the surface of 96-well microtitre plates using immunogold/silver staining techniques. The method is sufficiently straightforward to allow, for the first time, assessment of secretory cells in the clinical laboratory. This approach has great potential for enumerating cells that secrete other products, such as the interleukins.
Walker
A G
AG
Biotechnology Group, Harwell Laboratory, U.K.
Dawe
K I
KI
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000921:Antibody-Producing Cells / Q000276:immunology*; D006825:Hybridomas; D007150:Immunohistochemistry; D051379:Mice
D000906:Antibodies