title: Effect of cold environment on hepatic microsomal delta 6 and delta 9 desaturase activity of male rats.
abstract: Male rats maintained at 24 C and then shifted to 5 C for 5 days increased food intake and decreased in growth rate and food conversion. No modification was observed in delta 6 desaturase activity, while delta 9 desaturase activity decreased after this period of time. These results were confirmed by liver microsomal and mitochondrial fatty acid composition. The phospholipid composition of liver microsomes was unaltered, whereas in mitochondria, phosphatidylcholine and sphingomyelin decreased and phosphatidylethanolamine increased due to the cold environment. The influence of food intake and weight changes on fatty acid metabolism was studied using (i) rats maintained at 5 C with restricted food intake to match the food intake of those kept at 24 C with food ad libitum and (ii) rats maintained at 24 C whose food intake was also restricted so that their growth rate would be the same as that of rats maintained at 5 C with food ad libitum, respectively. These results indicate that the negative metabolic balance state of these cold conditions is not an active factor modifier of delta 6 desaturase activity, whereas it decreases delta 9 desaturase activity, reflecting the lipogenic characteristics of the latter enzyme.
authors: Instituto de Investigaciones Bioquimicas de La Plata, CONICET-UNLP, Facultad de Ciencias Medicas, Argentina.
authors: A
authors: A
authors: Ves Losada
authors: R O
authors: RO
authors: Peluffo
mesh_terms: D000064:Acclimatization; D000818:Animals; D003080:Cold Temperature; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D050602:Linoleoyl-CoA Desaturase; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013230:Stearoyl-CoA Desaturase
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D050602:Linoleoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase