title: The brain of Lymnaea contains a family of FMRFamide-like peptides.
abstract: Authentic FMRFamide and two FMRFamide-related heptapeptides were purified from the central nervous system of the fresh water snail Lymnaea stagnalis. The sequences of the heptapeptides were determined as: Ser-Asp-Pro-Phe-Leu-Arg-Phe-NH2 (SDPFLRFamide) and Gly-Asp-Pro-Phe-Leu-Arg-Phe-NH2 (GDPFLRFamide) by modified Edman degradation and enzymatic digestion. Relatively high quantities of the deamidated and therefore non-immunoreactive analogs of these two peptides (SDPFLRF and GDPFLRF) were also found. SDPFLRFamide and GDPFLRFamide were synthesized and were found to be chromatographically and biologically indistinguishable from the natural peptides, confirming the sequences. The log dose-response curves for the chronotropic action of either synthetic peptide on the heart of Lymnaea was very similar to that of FMRFamide. These data indicate that Lymnaea contains a family of FMRFamide-like peptides.
authors: Biological Laboratory, Vrije Universiteit, Amsterdam, The Netherlands.
authors: R H
authors: RH
authors: Ebberink
authors: D A
authors: DA
authors: Price
authors: H
authors: H
authors: van Loenhout
authors: K E
authors: KE
authors: Doble
authors: J P
authors: JP
authors: Riehm
authors: W P
authors: WP
authors: Geraerts
authors: M J
authors: MJ
authors: Greenberg
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D019835:FMRFamide; D008195:Lymnaea / Q000378:metabolism*; D009479:Neuropeptides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis; D011863:Radioimmunoassay
chemical_list: D000596:Amino Acids; D009479:Neuropeptides; D010455:Peptides; D019835:FMRFamide