Amiloride-sensitive net Mg2+ efflux from isolated perfused rat hearts.
Coronary blood vessels of isolated spontaneously beating rat hearts were perfused with an Mg2+-free solution at a rate of 3 ml/min. 1.5-ml fractions were sampled. Net Mg2+ efflux from heart muscle cells was measured by the increase of Mg2+ concentration in the perfusate. Addition of 0.3 microM isoproterenol to the perfusate resulted in a rapidly occurring transient Mg2+ efflux which was inhibited by amiloride.
Vormann
J
J
Institute of Molecular Biology and Biochemistry, Free University of Berlin.
Günther
T
T
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013054:Spectrophotometry, Atomic
D000584:Amiloride; D008274:Magnesium; D007545:Isoproterenol