The lack of attachment of transformed embryonic lung epithelial cells to collagen I is corrected by fibronectin and FXIII.
PER cells, a transformed pulmonary epithelial cell line that adhered to a large extent to a fibronectin substratum, were found to be attachment-deficient to collagen I. Although fibronectin can bind to collagen I monomers and polymers, the addition of exogenous fibronectin in the attachment medium induced the adhesion of these cells to collagen I polymers but not to monomers. By adding the transglutaminase of blood coagulation, FXIII, in the presence of fibronectin, the attachment of PER cells to collagen I monomers could be recovered while the minimal concentration of fibronectin needed to promote their adhesion to polymers was lowered. These studies indicate that FXIII enhances the fibronectin-mediated attachment of PER cells to collagen I.
Paye
M
M
Laboratoire de Dermatologie Expérimentale, Université de Liège, Belgium.
Lapière
C M
CM
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line / Q000502:physiology*; D002471:Cell Transformation, Neoplastic; D003094:Collagen / Q000378:metabolism*; D004848:Epithelium / Q000196:embryology; D005176:Factor XIII / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D008168:Lung / Q000196:embryology; D051381:Rats
D005353:Fibronectins; D003094:Collagen; D005176:Factor XIII