Stabilization of human prostate acid phosphatase by cross-linking with diimidoesters.
1. Modification of dimeric human prostate acid phosphatase (EC 3.1.3.2) by diimidoesters leads to the formation of water-soluble preparations of high enzymatic activity, resistant to denaturing agents. 2. Monomeric, dimeric, trimeric and tetrameric species were found in SDS-polyacrylamide gel electrophoresis of the phosphatase cross-linked with dimethyl-suberimidate, and dimeric, trimeric and tetrameric enzymatically active species on thin-layer Sephadex 200 gel filtration. This molecular pattern evidenced formation of the inter-subunit covalent linkages. All molecular forms are immunoreactive against the polyclonal rabbit anti-phosphatase antibodies. 3. The catalytic properties of the modified phosphatase are almost the same as those of the native enzyme. Differences in the optical properties between the modified and the native enzymes point to slight conformational transitions in the modified enzyme.
Wasylewska
E
E
Institute of Medical Biochemistry, N. Copernicus Academy of Medicine, Kraków, Poland.
Dulińska
J
J
Trubetskoy
V S
VS
Torchilin
V P
VP
Ostrowski
W S
WS
D000135:Acid Phosphatase / Q000302:isolation & purification; D000328:Adult; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006801:Humans; D007096:Imidoesters; D008297:Male; D008968:Molecular Conformation; D011467:Prostate / Q000201:enzymology*; D011489:Protein Denaturation; D013057:Spectrum Analysis
D003432:Cross-Linking Reagents; D007096:Imidoesters; D000135:Acid Phosphatase