Lactate accumulation in ischemic- and anoxic-isolated rat hearts assessed by H-1 spectroscopy.
Proton magnetic resonance (MR) spectroscopy with homonuclear editing was used to assess lactate accumulation in ischemic and anoxic hearts perfused according to the Langendorff method. An increase in the lactate signal was observed after 5 minutes of ischemia and after 10 to 20 minutes of anoxia. This study demonstrates that proton MR spectroscopy can be used to monitor the time course of MR-visible lactate levels in ischemic and anoxic isolated rat hearts.
Richards
T L
TL
Department of Radiology, University of California School of Medicine, San Francisco 94143.
Terrier
F
F
Sievers
R E
RE
Lipton
M J
MJ
Moseley
M E
ME
Higgins
C B
CB
D000818:Animals; D003327:Coronary Disease / Q000175:diagnosis*; D007773:Lactates / Q000032:analysis*; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000473:pathology*; D010100:Oxygen; D051381:Rats; D011919:Rats, Inbred Strains
D007773:Lactates; D019344:Lactic Acid; D010100:Oxygen