Synthesis and properties of vinyl monomer/enzyme conjugates. Conjugation of L-asparaginase with N-succinimidyl acrylate.
Monomer conjugation of an enzyme followed by copolymerization with free monomer is a useful method of enzyme immobilization. L-asparaginase was conjugated with N-succinimidyl acrylate. Analysis of the conjugated enzyme via isoelectric focusing showed that a molar ratio of 9.5 free monomers per enzyme was needed during the conjunction for each vinyl group bound. Only 3% of the enzyme activity was lost per vinyl group added, and conjugation of an average of four monomers per enzyme thermally destabilized the enzyme only at temperatures above 50 degrees C. Activity of the enzyme at physiological temperatures was relatively unaffected.
Shoemaker
S G
SG
Center for Bioengineering, University of Washington 98195.
Hoffman
A S
AS
Priest
J H
JH
D000179:Acrylates; D001215:Asparaginase / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D008970:Molecular Weight; D013388:Succinimides
D000179:Acrylates; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D013388:Succinimides; C053911:N-succinimidyl acrylate; D001215:Asparaginase