title: Immunohistochemical localization of laminin, neural cell adhesion molecule, collagen type IV and T-61 antigen in the embryonic retina of the Japanese quail by in vivo injection of antibodies.
abstract: Antibodies against laminin (LN), fibronectin (FN), collagen type IV (Col IV), neural cell adhesion molecule (N-CAM), T-61 antigen, actin, tubulin and neurofilament protein were injected into the eyes of quail embryos (Coturnix coturnix japonica) of different ages. Twenty h after injection, the heads of the embryos were fixed and the antibodies visualized in sections with the use of fluorescein-isothiocyanate (FITC) or peroxidase-labeled second antibodies by light- and electron microscopy. Antibodies against cell surface molecules, such as N-CAM, LN, Col IV and T 61, labeled matrix and membrane components of the retinal cells in different antigen-specific patterns. Antibodies against intracellular antigens, such as actin, tubulin and neurofilament protein labeled nonspecifically the vitreous body and the inner basal lamina of the retina, but resulted in only a very weak and diffuse labeling of retinal cells. N-CAM was detected in high concentration in the optic fiber layer on the surface of axons and on the membranes of all retinal cells. Col IV, LN and T 61 antigen were found predominantly in the optic fiber layer. LN and Col IV were located on the surface of axons and the endfeet of ventricular (neuroepithelial) cells in a patchy distribution. The T-61 antigen was found in early stages in the cell-free space of the optic fiber layer, on the surface of ventricular cells and axons, and at later stages also in high-density patches between nerve fibers. The distribution of LN and T-61 antigen together with data from in vitro experiments suggests a crucial role of these proteins in axon extension in the avian retina during early development of the optic fiber layer.
authors: Max-Planck Guest Laboratory, Institute of Cell Biology, Academia Sinica, Shanghai, China.
authors: W
authors: W
authors: Halfter
authors: C S
authors: CS
authors: Fua
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D003094:Collagen / Q000032:analysis*; D003370:Coturnix; D004625:Embryo, Nonmammalian; D005136:Eye Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D012160:Retina / Q000166:cytology* / Q000196:embryology / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D005136:Eye Proteins; D007797:Laminin; D008565:Membrane Proteins; D003094:Collagen