title: Use of biotinylated probes in serum hepatitis B virus DNA detection.
abstract: A dot-blot hybridisation assay for serum HBV DNA is described using a non-radioactive (biotinylated) DNA probe. The assay is both sensitive (1 pg of HBV DNA) and reproducible, and shows several advantages over similar assays which use 32P-labelled probes for the routine detection of infectious virus particles both in blood and in other biological fluids.
authors: Department of Physiology, Royal Free Hospital School of Medicine, London, U.K.
authors: J A
authors: JA
authors: Saldanha
authors: P
authors: P
authors: Karayiannis
authors: H C
authors: HC
authors: Thomas
authors: J P
authors: JP
authors: Monjardino
mesh_terms: D001710:Biotin; D004279:DNA, Viral / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011933:Reagent Kits, Diagnostic
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic; D001710:Biotin