Heat stability of lyophilized C1 inactivator concentrates.
Heat stability of lyophilized C1 inactivator (C1-INA) concentrates of intermediate and high purity has been investigated under several heat treatment protocols that include heating for 96 and 192 h at 68 degrees C and for 10 h at 80, 90 and 100 degrees C. Both types of concentrate showed high stability in functional activity, with not more than 5% loss in any of the time-temperature combinations evaluated. However, the C1-INA antigen from both concentrates showed small but progressive changes in crossed immunoelectrophoretic pattern, in proportion to the intensity of heat treatment. High-pressure size-exclusion chromatography revealed only minimal signs of aggregation in the high-purity concentrate, but a significant and progressive aggregation of nonspecific protein contaminants present in the intermediate-purity concentrate, making the high-purity concentrate preferable for heat treatment.
Wickerhauser
M
M
Plasma Derivatives Laboratory, American Red Cross Laboratories, Rockville, Md.
Williams
C
C
Kolen
B L
BL
Busby
T F
TF
D003174:Complement C1 Inactivator Proteins / Q000302:isolation & purification; D004355:Drug Stability; D005612:Freeze Drying; D006358:Hot Temperature; D006801:Humans
D003174:Complement C1 Inactivator Proteins