[Effect of the lipophilic spin-labeled inhibitor of cytochrome P-450 on the activity of the microsomal system].
It was shown that the lipophilic nitroxyl radical--2-hexyl-2,3,5,5-tetramethyl-4-(3-iodo-2-oxopropyliden)-im idazolidine- 1-oxyl, an affinity modified of rat liver microsomal cytochrome P-450, interacts with various forms of cytochrome P-450 as substrate type I, and it inhibits the oxidation of substrates specific for these forms. During its intravenous injection with egg phosphatidylcholine liposomes the radical is partly bound to liver microsomes, which is accompanied by a decrease of the oxygenase activity of microsomal preparations (by 30-50%) as well as by prolongation of the soporific effect of hexabarbital (2-3-fold).
Subkhankulova
T N
TN
Liakhovich
V V
VV
Vaĭner
L M
LM
D000818:Animals; D003497:Cyclic N-Oxides / Q000378:metabolism / Q000494:pharmacology*; D065607:Cytochrome P-450 Enzyme Inhibitors; D006591:Hexobarbital; D006900:Hydroxylation; D007093:Imidazoles / Q000378:metabolism / Q000494:pharmacology*; D048289:Imidazolidines; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology* / Q000378:metabolism; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012890:Sleep / Q000187:drug effects; D013113:Spin Labels; D013379:Substrate Specificity
D003497:Cyclic N-Oxides; D065607:Cytochrome P-450 Enzyme Inhibitors; D007093:Imidazoles; D048289:Imidazolidines; D010743:Phospholipids; D013113:Spin Labels; C053920:2-hexyl-2,3,5,5-tetramethyl-4-(3-iodo-2-oxopropylidene)imidazolidine-1-oxyl; D006591:Hexobarbital
Vliianie lipofil'nogo spin-mechenogo ingibitora tsitokhroma P-450 na aktivnost' mikrosomal'noĭ sistemy.