Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions.
Bovine anterior-pituitary microsomal fractions exhibit high-affinity, saturable and reversible binding of inositol 1,4,5-[32P]trisphosphate; 50% of the labelled ligand is displaced by 3.5 nM-inositol 1,4,5-trisphosphate. 0.5 microM-inositol 1,4-bisphosphate and 10 microM-ATP. Inositol 1,4,5-trisphosphate induces the release of Ca2+ from the microsomal vesicles (half-maximal effect at 290 nM), and its action is potentiated by inositol tetrakisphosphate (half-maximal effect at 4 microM).
Spät
A
A
Department of Physilogy, Semmelweis University Medical School, Budapest, Hungary.
Lukács
G L
GL
Eberhardt
I
I
Kiesel
L
L
Runnebaum
B
B
D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D007295:Inositol Phosphates / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism*
D007295:Inositol Phosphates; D013403:Sugar Phosphates; D002118:Calcium