Control of the action of phospholipases A by "vertical compression" of the substrate monolayer.
Monolayers of rac-1,2-didodecanoyl-sn-glycero-3-phosphoglycerol at an air-water interface were "vertically compressed" by substituting an alkylated glass plate for air while maintaining a constant surface pressure of 15 mN m-1. At this surface pressure the overlaying of the lipid film by the alkylated surface resulted in an average increase of 16 A2/molecule in the mean molecular area of those phospholipid molecules residing at the interface between water and the alkylated glass. Subsequently, the activities of phospholipases A1 and A2 toward the monolayers were measured both in the presence and in the absence of the support. While phospholipase A1 activity was increased 4-fold by the support, the activity of phospholipase A2 was reduced to 15% of the activity measured in the absence of the alkylated surface. These findings indicate that such a "vertical compression" of the monolayer is likely to induce a conformational change in the phospholipid molecules, which in turn would cause the above reciprocal changes in the activities of phospholipases A1 and A2. A molecular model accounting to these findings is presented.
Thuren
T
T
Department of Medical Chemistry, University of Helsinki, Finland.
Virtanen
J A
JA
Kinnunen
P K
PK
D000818:Animals; D007700:Kinetics; D008081:Liposomes; D010179:Pancreas / Q000201:enzymology; D010715:Phosphatidylglycerols; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054466:Phospholipases A1; D054467:Phospholipases A2; D011312:Pressure; D013499:Surface Properties; D013552:Swine
D008081:Liposomes; D010715:Phosphatidylglycerols; C054349:1,2-didodecanoyl-glycero-3-phosphocholine; D010740:Phospholipases; D010741:Phospholipases A; D054466:Phospholipases A1; D054467:Phospholipases A2