[Isolation of cathepsin D from fish muscle tissue].
A preparation of cathepsin D was isolated with a 20% yield from the Pacific herring muscles using thermal and acid-alkaline treatment as well as salting out with ammonium sulfate and gel chromatography on Sephadex G-75. Cathepsin D isolated from the Pacific sardine muscles was purified 200-400-fold by means of affinity chromatography on immobilized hemoglobin, the yield being about 5%.
Slutskaia
T N
TN
Igolkina
L A
LA
Sokolova
E V
EV
D000818:Animals; D002402:Cathepsin D / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D005399:Fishes; D005519:Food Preservation; D009132:Muscles / Q000201:enzymology*
D002402:Cathepsin D
Vydelenie katepsina D iz myshechnoĭ tkani ryb.