An azophenolic colorimetric reagent for use in enzyme-linked immunosorbent assays.
A colorimetric reagent, 4-(4'-nitro-2'-methylsulfonylphenylazo)phenyl phosphate (NMPP), has been shown to be an effective substrate of alkaline phosphatase. NMPP and p-nitrophenyl phosphate were applied in comparative studies using enzyme immunoassays for the detection of viral antigens and antiviral antibodies. The new substrate exhibited similar, or even higher, sensitivity than p-nitrophenyl phosphate depending on the substrate concentrations used. Positive and negative reactions were easier to define, even without cumbersome equipment. The enzyme reaction was terminated by the uncompetitive inhibitor, theophylline.
Dietzgen
R G
RG
Department of Plant Pathology, Cornell University, Ithaca, New York 14853.
Snoke
R E
RE
Babb
B
B
D000469:Alkaline Phosphatase; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001391:Azo Compounds; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D007202:Indicators and Reagents; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D013379:Substrate Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral; D001391:Azo Compounds; D007202:Indicators and Reagents; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C053982:4-(4'-nitro-2'-methylsulfonylphenylazo)phenyl phosphate; C008644:nitrophenylphosphate; D000469:Alkaline Phosphatase