title: Evidence of a selective major vascular marginal pool of lymphocytes in the lung.
abstract: The size and mobilization of the pulmonary vascular pool of lymphocytes was studied in young pigs. Blood lymphocytes, labeled in vitro with a fluorescent dye, were injected either intravenously or into the left heart. Comparable high numbers of lymphocytes were found in the lung after 30 min, demonstrating a specific homing of lymphocytes to the lung vascular bed. When the left lung was perfused with a cell-free medium for as long as 4 h in vitro, a continuous venous release of lymphocytes was found that resulted in about 1.5 x 10(9) lymphocytes and 5.7 x 10(6)/min leukocytes. Peripheral blood lymphocytes were labeled with 51Cr in vitro and injected intravenously. Fifteen minutes or 1 day later the lungs were perfused, and the release of cell-bound radioactivity was measured. The data indicate the existence of a large intravascular pulmonary lymphocyte pool. The sequestration of lymphocytes in the lung in many lymphocyte migration studies is not a removal of effete cells but a physiologic phenomenon.
authors: Centre of Anatomy, Medical School of Hannover, FRG.
authors: R
authors: R
authors: Pabst
authors: R M
authors: RM
authors: Binns
authors: S T
authors: ST
authors: Licence
authors: M
authors: M
authors: Peter
mesh_terms: D000818:Animals; D001803:Blood Transfusion / Q000379:methods; D002860:Chromium Radioisotopes; D066298:In Vitro Techniques; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D007958:Leukocyte Count; D008168:Lung / Q000276:immunology*; D017710:Lymphocyte Transfusion; D008214:Lymphocytes; D010477:Perfusion; D013552:Swine
chemical_list: D002860:Chromium Radioisotopes