title: Subunit dissociation and activation of wild-type and mutant glucocorticoid receptors.
abstract: Apparent molecular weights of wild-type and nti ('increased nuclear transfer') mutant glucocorticoid receptors were obtained from Stokes radii and sedimentation coefficients. At low salt concentrations molecular forms of Mr 328,000 and 298,000 of the wild-type and mutant, respectively, were predominant. Increasing ionic strength resulted in receptor dissociation. Dissociated forms of Mr 130,000 and 63,000 of the wild-type and mutant, respectively, were obtained at 300 mM KCl and above. Some metal oxi-anions prevented dissociation. Receptor activation to allow DNA binding produced the dissociated forms which could be separated from non-activated receptors by filtration through DNA-cellulose or by DEAE-cellulose chromatography. Non-activated wild-type and nti receptors eluted from DEAE-cellulose under identical conditions while activated wild-type and nti receptors eluted differently. Partially proteolyzed wild-type receptors behaved identically to nti receptors. We conclude that the large forms of wild-type and nti receptors are heteromeric and contain only one hormone-building polypeptide per complex.
authors: Institut für Biologische Chemie der Universität, Heidelberg, F.R.G.
authors: U
authors: U
authors: Gehring
authors: K
authors: K
authors: Mugele
authors: H
authors: H
authors: Arndt
authors: W
authors: W
authors: Busch
mesh_terms: D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D008223:Lymphoma; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D011965:Receptors, Glucocorticoid / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D046911:Macromolecular Substances; D011965:Receptors, Glucocorticoid