Water-soluble and insoluble crystallins of the developing human fetal lens, analyzed by agarose/polyacrylamide thin-layer isoelectric focusing.
Eight human fetal lenses, selected on basis of normality, of a gestational age of 119 to 231 days were analyzed by thin-layer isoelectric focusing (IEF) in agarose/polyacrylamide gels. This method was adapted for the separation of lens crystallins into HM-, alpha-, beta- and gamma-crystallins. It is especially suitable for analysis under non-denaturing conditions of high-molecular-weight crystallins and of insoluble crystallins (WI) solubilized in formamide. The latter could be separated into HM-, alpha- and gamma-crystallins. During fetal development, a considerable increase of gamma-crystallin proportion was observed due to new synthesis. This increase was balanced by a decrease of alpha-crystallin proportion.
Ahrend
M H
MH
Department of Experimental Ophthalmology, University of Bonn, FRG.
Bours
J
J
Födisch
H J
HJ
D000180:Acrylic Resins; D003459:Crystallins / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism*; D005782:Gels; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007908:Lens, Crystalline / Q000196:embryology / Q000378:metabolism*; D012685:Sepharose; D012995:Solubility; D014867:Water; D000099166:Polyacrylamides
D000180:Acrylic Resins; D003459:Crystallins; D005782:Gels; D012685:Sepharose; D014867:Water; D000099166:Polyacrylamides