Measurement of acetylcholinesterase in erythrocytes in the field.
We describe here a field method we developed for colorimetry of erythrocytic acetylcholinesterase (EC 3.1.1.7) in capillary blood samples. Three stable, premixed assay reagents and de-ionized water (but no centrifuge or balance) are required. This method, adapted for a microplate format, is essentially that of Ellman et al. (Biochem Pharmacol 1961;7:88-95) as modified by George and Abernethy (Clin Chem 1983;29:365-8). Assays were quantified and corrected for hematocrit by using a battery-powered colorimeter with a silicon carbide (blue) light-emitting-diode source. Advantages over existing field methods include better portability, ruggedness, greater precision, and lower cost per sample.
Magnotti
R A
RA
Department of Internal Medicine, University of Cincinnati Medical Center, OH 45267-0585.
Eberly
J P
JP
Quarm
D E
DE
McConnell
R S
RS
D000110:Acetylcholinesterase / Q000097:blood*; D000293:Adolescent; D000328:Adult; D003124:Colorimetry; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D007306:Insecticides / Q000506:poisoning; D008297:Male; D008722:Methods; D009943:Organophosphorus Compounds; D011933:Reagent Kits, Diagnostic
D007306:Insecticides; D009943:Organophosphorus Compounds; D011933:Reagent Kits, Diagnostic; D000110:Acetylcholinesterase