A versatile and sensitive method for measuring oxygen.
Oxygen dependence of the lifetime of the excited triplet state of phosphorescent molecules can be used to measure the oxygen concentration in aqueous media. These measurements are insensitive to much of the optical interference that limits the usefulness of measurements based on the oxygen dependent quenching of luminescence intensity. The measurements also extend to significantly lower oxygen concentrations than are normally attainable using oxygen electrodes. The phosphorescence lifetimes can be accurately measured from a few microseconds to seconds, permitting a wide dynamic range of oxygen concentration measurements. With currently available probes, for example, it is possible to make continuous measurement of oxygen concentrations from 10(-4) M to 10(-8) M in a single experiment.
Wilson
D F
DF
Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104.
Vanderkooi
J M
JM
Green
T J
TJ
Maniara
G
G
DeFeo
S P
SP
Bloomgarden
D C
DC
D000818:Animals; D003306:Coproporphyrins; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008930:Mitochondria, Liver / Q000032:analysis / Q000378:metabolism; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption; D010165:Palladium; D051381:Rats
D003306:Coproporphyrins; D010165:Palladium; D010100:Oxygen