Simplified sodium dodecyl sulfate-polyacrylamide gel electrophoresis of unconcentrated urine with enhanced resolution and detection sensitivity.
We applied a simple sodium dodecyl sulfate-polyacrylamide gel electrophoresis method to urine. The method, developed for serum protein analysis (Clin Chem 1984;30:475-9), has a high sample throughput and gives excellent resolution with unconcentrated urine. It clearly distinguishes and characterizes proteinuric urine (7.5 microL) by Coomassie Blue staining and gives complex silver-stained patterns with nonproteinuric urine (2 microL). The former is recommended for routine clinical screening, the latter for research purposes.
Marshall
T
T
Biology Department, University of Ulster, Coleraine, Northern Ireland.
Williams
K M
KM
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D011507:Proteinuria / Q000652:urine; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; D014556:Urine / Q000032:analysis*
D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; C048139:Coomassie blue