Amino acid composition and NH2-terminal amino acid sequence of rat platelet secretory phospholipase A2.
The amino acid composition and partial NH2-terminal amino acid sequence of the phospholipase A2 secreted by stimulated rat platelets were determined. The most predominant amino acid in the phospholipase A2 was cysteine followed by lysine, suggesting that it is a basic one. This finding is consistent with its high affinity to a cation exchange column. The NH2-terminal 24 amino acids were found to be as follows: X-Leu-Leu-Glu-Phe-Gly-Gln-Met-Ile-Leu-Phe-Lys-Thr-Gly-Lys-Arg-Ala-Asp- Val-Ser-Tyr-Gly-Phe-Tyr-Gly- The enzymes contains 5Phe, 8Met, 9Ile, 24Tyr, and 25Gly residues, all of which are conserved in the sequenced pancreatic phospholipase A2. This is the first report of the tentative characterization of a eukaryotic phospholipase A2, the cellular source of which is known, i.e., it does not originate from a venom or the pancreas.
Hayakawa
M
M
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo.
Horigome
K
K
Kudo
I
I
Tomita
M
M
Nojima
S
S
Inoue
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001792:Blood Platelets / Q000201:enzymology*; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2