title: Incorporation of intraportal ammonia-N into blood and tissue nitrogenous compounds in chickens fed low and high protein diets.
abstract: 1. 15N-Percentage of the amide of glutamine in total blood non-protein-15N was 42 and 48% in chickens fed 5 and 20% protein diets, respectively, when 15N-ammonia was intraportally-infused for 6 hr. 2. The infused ammonia-15N also appeared in the amide of free glutamine in the liver and kidney in large amounts at both levels of protein intake. 3. The 15N incorporated into glutamine-amide in the blood, liver and kidney and non-protein-15N in plasma were greater in chickens fed the low protein diet than in those fed the high protein diet (P less than 0.05 except kidney of P less than 0.01). 4. About 60% of the amide-N of the glutamine which was increased during 6 hr infusion of ammonia was derived from infused ammonia-N and the remainder from endogenous nitrogen, irrespective of protein intake. 5. These results suggest that glutamine is the most important intermediate in detoxication of intraportal ammonia in chickens.
authors: Laboratory of Animal Nutrition and Feed Science, Faculty of Agriculture, Shinshu University, Nagano-ken, Japan.
authors: Y
authors: Y
authors: Karasawa
authors: K
authors: K
authors: Koh
mesh_terms: D000641:Ammonia / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D004044:Dietary Proteins / Q000494:pharmacology*; D005973:Glutamine / Q000096:biosynthesis; D007262:Infusions, Intravenous; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008102:Liver Circulation; D008297:Male; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes
chemical_list: D004044:Dietary Proteins; D009587:Nitrogen Isotopes; D005973:Glutamine; D000641:Ammonia; D009584:Nitrogen