Rapid and simple determination of sennoside A and B in rhei rhizoma by ion-pair high-performance liquid chromatography.
A simple and precise ion-pair high-performance liquid chromatographic method was developed for the determination of sennoside A and B in Rhei Rhizoma. A reversed-phase chromatographic system consisting of a chemically bonded ODS silica gel column with an acetate buffer (pH 5.0)-acetonitrile (68:32), containing 5 mM tetra-n-heptylammonium bromide, as the mobile phase was used. Sennoside A and B in this crude drug were separated and determined within 20 min after direct injection of the solution extracted with 70% methanol. The results for various samples are presented.
Sagara
K
K
Research Center, Taisho Pharmaceutical Co. Ltd., Saitama, Japan.
Oshima
T
T
Yoshida
T
T
D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010946:Plants, Medicinal / Q000032:analysis*; D012250:Rheum / Q000032:analysis*; D012676:Senna Extract; D000081226:Sennosides
D000880:Anthraquinones; D000081226:Sennosides; D012676:Senna Extract