Visualization of pig sperm chromosomes by in-vitro penetration of zona-free hamster ova.
Zona-free hamster eggs were penetrated by pig spermatozoa capacitated using bovine follicular fluid and Percoll gradients. A mean penetration rate of 80.1% was obtained from 5 ejaculates from 2 boars. Penetrated eggs were cultured and analysable metaphase chromosome spreads were obtained from 16.8%. The analysis of 20 pig sperm complements indicated that 9 were 19, Y, 10 were 19, X and 1 appeared to have an XY sex chromosome constitution.
Creighton
P
P
Department of Microbiology, University College, Galway, Ireland.
Houghton
J A
JA
D000818:Animals; D002499:Centrifugation, Density Gradient; D002875:Chromosomes / Q000648:ultrastructure*; D006224:Cricetinae; D005260:Female; D008297:Male; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000648:ultrastructure*; D013552:Swine / Q000235:genetics*; D014960:X Chromosome / Q000648:ultrastructure; D014998:Y Chromosome / Q000648:ultrastructure