Relocation of nonalbumin proteins after albumin resuscitation.
Prior work showed that albumin (5% A) resuscitation lowered serum globulins and coagulation protein activity levels compared to balanced electrolyte solution (BES) resuscitation. In this experiment, the effect of 1.25% A in BES (A-BES) on serum and lymphatic levels of nonalbumin proteins was studied in 20 splenectomized dogs subjected to reservoir shock and then treated with (a) BES or A-BES, (b) shed blood, and (c) autogenous bank blood. Serum levels of albumin, globulin, and coagulation protein activity were measured preshock, postshock, and daily for 3 days; skin lymph levels were measured on Day 3. Compared to BES, A-BES resuscitation significantly decreased serum globulins and coagulation protein activity of fibrinogen, Factor VIII, and antithrombin III. In contrast, skin lymph globulins and coagulation protein activity levels increased significantly with A-BES compared to BES resuscitation. These data suggest that the serum protein changes seen with albumin-supplemented resuscitation result from extravascular relocation of nonalbumin proteins, possibly, due to an oncotic homeostatic factor.
Denis
R
R
Department of Surgery, Wayne State University, Detroit, Michigan 48201.
Smith
R W
RW
Grabow
D
D
Ledgerwood
A M
AM
Lucas
C E
CE
D000818:Animals; D001779:Blood Coagulation Factors / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D004285:Dogs; D005440:Fluid Therapy; D012151:Resuscitation; D012709:Serum Albumin / Q000627:therapeutic use*; D012771:Shock, Hemorrhagic / Q000097:blood / Q000628:therapy
D001779:Blood Coagulation Factors; D001798:Blood Proteins; D012709:Serum Albumin