Studies on the mechanism of acetaldehyde-mediated inhibition of rat liver transaminases.
Incubation of mitochondria-depleted rat liver homogenates with 5 mmol/l acetaldehyde at 37 degrees C for 1 h inhibited both aspartate and alanine aminotransferases by 30%. Inhibition was prevented by decreasing temperature to 4 degrees C or by preincubating homogenates with cyanate but was unaffected by cyanamide and methylpyrazole which block acetaldehyde oxidation and reduction respectively. Cyanate-sensitive acetaldehyde-mediated inhibition of purified porcine heart transaminases was also demonstrated in the presence of rat liver homogenate but not in Tris/sucrose medium. Moreover, porcine transaminases were inhibited by trichloroacetic acid extracts of rat liver homogenates previously incubated with acetaldehyde but not by extracts of homogenates incubated with both acetaldehyde and cyanate. These findings suggest that acetaldehyde-mediated transaminase inhibition requires further non-oxidative metabolism of acetaldehyde. Since transaminase activities were not restored by addition of pyridoxal 5'-phosphate to the assay systems, acetaldehyde-induced transaminase inhibition does not appear to be mediated by displacement or depletion of this B6 coenzyme.
Solomon
L R
LR
Department of Medicine, Veterans Administration Medical Center, West Haven, CT 06516.
D000079:Acetaldehyde / Q000494:pharmacology*; D000818:Animals; D008929:Mitochondria, Heart / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D011736:Pyridoxine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D000637:Transaminases / Q000037:antagonists & inhibitors*; D014238:Trichloroacetic Acid
D014238:Trichloroacetic Acid; D000637:Transaminases; D000079:Acetaldehyde; D011736:Pyridoxine