title: Magnetic particle motions within living cells. Measurement of cytoplasmic viscosity and motile activity.
abstract: Submicrometer magnetic particles, ingested by cells and monitored via the magnetic fields they generate, provide an alternative to optical microscopy for probing movement and viscosity of living cytoplasm, and can be used for cells both in vitro and in vivo. We present methods for preparing lung macrophages tagged with magnetic particles for magnetometric study. Interpretation of the data involves fitting experimental remanent-field decay curves to nonlinear mechanistic models of intracellular particle motion. The model parameters are sensitive to mobility and apparent cytoplasmic viscosity experienced by particle-containing organelles. We present results of parameter estimation for intracellular particle behavior both within control cells and after (a) variable magnetization duration, (b) incubation with cytochalasin D, and (c) particle twisting by external fields. Magnetometric analysis showed cytoplasmic elasticity, dose-dependent motion inhibition by cytochalasin D, and a shear-thinning apparent viscosity.
authors: Department of Environmental Science and Physiology, Harvard School of Public Health, Boston, Massachusetts 02115.
authors: P A
authors: PA
authors: Valberg
authors: H A
authors: HA
authors: Feldman
mesh_terms: D000818:Animals; D006224:Cricetinae; D003593:Cytoplasm / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D005290:Ferric Compounds / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008280:Magnetics; D008433:Mathematics; D008854:Microscopy, Electron / Q000379:methods; D008954:Models, Biological; D014783:Viscosity
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide