title: Effect of NH4+ ions on phi 29 DNA-protein p3 replication: formation of a complex between the terminal protein and the DNA polymerase.
abstract: Ammonium ions stimulated the formation of the phi diameter 29 protein p3-dAMP initiation complex by decreasing the Km value for dATP in a purified system containing the viral terminal protein p3, the viral DNA polymerase p2, and the phi 29 DNA-protein p3 complex as a template. In addition, NH4+ ions stimulated the amount of p3-dAMP complex elongation and increased by about twofold the rate of elongation. The stimulatory effect of NH4+ ions on in vitro phi 29 DNA replication is probably related to the formation of a stable complex between the terminal protein and the DNA polymerase, which was detected only in the presence of NH4+ ions.
authors: Centro de Biología Molecular, Universidad Autónoma de Madrid, Spain.
authors: L
authors: L
authors: Blanco
authors: I
authors: I
authors: Prieto
authors: J
authors: J
authors: Gutiérrez
authors: A
authors: A
authors: Bernad
authors: J M
authors: JM
authors: Lázaro
authors: J M
authors: JM
authors: Hermoso
authors: M
authors: M
authors: Salas
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology*; D001435:Bacteriophages / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D007700:Kinetics; D013698:Templates, Genetic; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D004279:DNA, Viral; D003838:Deoxyadenine Nucleotides; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase; D000645:Ammonium Sulfate