title: In vitro cultivation of rickettsia-like-organisms from Glossina spp.
abstract: A method is described for the in vitro cultivation of the rickettsia-like-organisms (RLO) from Glossina spp. which are believed to be associated with susceptibility to trypanosome infection. Cultures of RLO were established by infecting a mosquito cell line (Aedes albopictus) with haemolymph taken from teneral flies. RLO from nine species of Glossina have been isolated and maintained in continuous culture using this technique.
authors: Tsetse Research Laboratory, University of Bristol, Langford, U.K.
authors: S C
authors: SC
authors: Welburn
authors: I
authors: I
authors: Maudlin
authors: D S
authors: DS
authors: Ellis
mesh_terms: D000818:Animals; D006434:Hemocytes / Q000648:ultrastructure; D008854:Microscopy, Electron; D012281:Rickettsia / Q000254:growth & development* / Q000302:isolation & purification / Q000648:ultrastructure; D014370:Tsetse Flies / Q000382:microbiology* / Q000648:ultrastructure