Oxygen-sensitive 19F NMR imaging of the vascular system in vivo.
The fluorine nuclear magnetic resonance spin-lattice relaxation rate (1/T1) of the perfluorochemical blood substitute perfluorotripropylamine (FTPA) is very sensitive to oxygen tension. This presents the possibility of measuring blood oxygen tension by 19F MR imaging. We obtained oxygen-sensitive 19F NMR images of the circulatory system of rats infused with emulsified FTPA. Blood oxygenation was assessed under conditions of both air- and 100% O2-breathing. T1 relaxation times were derived from MR images using a partial saturation pulse sequence. The T1 times were compared with a phantom calibration curve to calculate average blood pO2 values in the lung, liver, and spleen. The results showed marked, organ-specific increases in blood oxygen tension when the rat breathed 100% O2 instead of air.
Fishman
J E
JE
Pendergrass Diagnostic Radiology Research Laboratory, Department of Radiology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Joseph
P M
PM
Floyd
T F
TF
Mukherji
B
B
Sloviter
H A
HA
D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology*; D005461:Fluorine; D005466:Fluorocarbons; D006321:Heart / Q000033:anatomy & histology; D008099:Liver / Q000098:blood supply; D008168:Lung / Q000098:blood supply; D008279:Magnetic Resonance Imaging; D010100:Oxygen / Q000097:blood*; D051381:Rats; D013154:Spleen / Q000098:blood supply
D005466:Fluorocarbons; D005461:Fluorine; C039422:perfluorotripropylamine; D010100:Oxygen