title: Membrane-associated sickle hemoglobin: a major determinant of sickle erythrocyte rigidity.
abstract: Micropipette aspiration tests on single erythrocytes have previously shown that the static rigidity (membrane shear modulus) of oxygenated sickle cells increased with increasing hemoglobin concentration, whereas the rigidity of normal cells was independent of hemoglobin concentration. Moreover, it was observed that after mechanical extension, sickle cells exhibited persistent deformation more frequently and to a greater extent than normal cells. To ascertain if differences in association of normal and sickle hemoglobin with the membrane could account for these observations, we measured rheologic properties of normal membranes reconstituted with sickle hemoglobin and sickle membranes reconstituted with normal hemoglobin. The static rigidity of normal ghosts reloaded with sickle hemoglobin was higher than those of either normal ghosts reloaded with normal hemoglobin or native normal cells. On the other hand, the increased rigidity of native sickle cells decreased to near-normal values following reconstitution with normal hemoglobin. Furthermore, we observed that normal ghosts reconstituted with sickle hemoglobin exhibited persistent bumps after mechanical extension, but no bumps formed on normal ghosts reconstituted with normal hemoglobin. Moreover residual bumps were not produced on sickle cells reloaded with normal hemoglobin. Since mechanical characteristics peculiar to sickle cells could be induced in normal cells by incorporation of sickle hemoglobin, and since normal characteristics could be restored to sickle cells by incorporation of normal hemoglobin, we suggest that the interaction of sickle hemoglobin with the cell membrane is responsible for augmented static rigidity of oxygenated sickle erythrocytes.
authors: Department of Pathology, University of British Columbia, Vancouver, Canada.
authors: E A
authors: EA
authors: Evans
authors: N
authors: N
authors: Mohandas
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000648:ultrastructure*; D006451:Hemoglobin, Sickle / Q000502:physiology*; D006801:Humans; D010108:Oxyhemoglobins / Q000502:physiology; D012016:Reference Values; D012212:Rheology
chemical_list: D006451:Hemoglobin, Sickle; D010108:Oxyhemoglobins; C029775:oxyhemoglobin S