Production and partial characterization of monoclonal antibodies against 3,3',5-triiodo-L-thyronine.
Spleen cells of Biozzi HL mouse (selection V) immunized with bovine albumin-triiodothyronine conjugate were fused with P3-X63-Ag8.653 mouse myeloma cells. Thirteen monoclonal antibodies, selected by an enzyme-linked immunosorbent assay and a radioimmunoassay, were produced in mouse ascites fluid, purified and analyzed. All of them were IgG1 (kappa). The cross-reactivity of all these monoclonal antibodies with thyroxine (T4) was less than 0.2%. The association constant determined by Scatchard analysis ranged from 1.5 x 10(9) M-1 to 2.7 x 10(10) M-1.
Marchand
J
J
Centre de Recherches Clin-Midy, Groupe Sanofi, Montpellier, France.
Gorce
A
A
Badouaille
G
G
Bras
J M
JM
Simon
D
D
Pau
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000032:analysis; D002460:Cell Line; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D010954:Plasmacytoma; D011863:Radioimmunoassay; D014284:Triiodothyronine / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D014284:Triiodothyronine