Linear multidimensional liquid chromatography in the preparative scale purification of calmodulin from brain extract.
Rapid preparative scale purification of calmodulin from crude bovine brain extract is achieved in a single chromatographic run by physically coupling two different liquid chromatography columns which employ different separation mechanisms. In this case columns packed with newly commercialized 40-microns silica-based hydrophobic interaction and 5-microns micron silica-based weak anion-exchange chromatography media were used. The only sample preparation required for conducting this purification procedure is the addition of salt to the crude brain supernatant to promote the initial binding of calmodulin to the hydrophobic interaction chromatography media. Chromatography carried out on such linear arrangements of columns has been referred to as linear multidimensional liquid chromatography.
Berkowitz
S A
SA
J. T. Baker Chemical Company, Phillipsburg, New Jersey 08865.
D000818:Animals; D001923:Brain Chemistry; D002147:Calmodulin / Q000302:isolation & purification*; D002417:Cattle; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration
D002147:Calmodulin