Complete amino acid sequence of an immunoreactive form of human pancreatic stone protein isolated from pancreatic juice.
The primary structure of a pancreatic stone protein form has been elucidated for the first time. The protein studied was the lowest-Mr form prepared from human pancreatic juice (PSP S1). The N-terminal sequence up to residue 65 had already been determined. The five peptides obtained after staphylococcal protease digestion of the carboxymethylated reduced and succinylated PSP S1 enabled the deduction of the entire sequence. The tryptic peptides arising from the digest of cyclohexanedione--treated PSP S1 and the amino acids released by carboxypeptidase P digestion of PSP S1 confirmed the data of the sequence. The peptides were purified by Sephadex filtration and, if required, by chromatography on DEAE-cellulose or thin-layer cellulose. The amino acid sequences of the peptides were determined with a sequencer. From the sequence data it was deduced that the PSP S1 polypeptide chain contains 133 amino acid residues and has a Mr of 15,000.
De Caro
A M
AM
Centre de Biochimie et de Biologie Moléculaire du CNRS, Marseille, France.
Bonicel
J J
JJ
Rouimi
P
P
De Caro
J D
JD
Sarles
H
H
Rovery
M
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001322:Autoanalysis; D002135:Calcium-Binding Proteins / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D006801:Humans; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010189:Pancreatic Juice / Q000032:analysis* / Q000276:immunology
D000596:Amino Acids; D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human