Serum-free serial culture of adult human keratinocytes from suction-blister roof epidermis.
Coating cell culture flasks with natural extracellular matrix (ECM) enhanced the culture of adult human keratinocytes from suction-blister roof epidermis in an environment without fetal calf serum (FCS), bovine pituitary extracts or cellular feeder layers. A higher incidence of cell attachment on natural ECM was observed than on collagen and human fibronectins (HFN)-coated plastic dishes, and natural ECM was necessary for growth and proliferation of attached cells under the culture conditions used. Cells in primary culture grew to confluency on natural ECM-coated surfaces within about 14 days, and subsequent serial passage could be made up to fourth passage in collagen- and HFN-coated plastic flasks. Cultured keratinocytes in this serum-free environment formed colonies of small cuboidal, healthy cells with little keratinization or stratification and demonstrated antigenic characteristics of human basal cells.
Furukawa
F
F
Department of Dermatology, University of Colorado School of Medicine, Denver 80262.
Huff
J C
JC
Weston
W L
WL
Norris
D A
DA
D000293:Adolescent; D000328:Adult; D001768:Blister / Q000473:pathology; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D004817:Epidermis / Q000473:pathology*; D005109:Extracellular Matrix; D005260:Female; D005353:Fibronectins; D006801:Humans; D008297:Male; D008875:Middle Aged; D010969:Plastics
D003470:Culture Media; D005353:Fibronectins; D010969:Plastics; D003094:Collagen