Dissociation of muscle cells from the bony fish heart.
A method for dissociating myocardial cells from the bony fish heart is presented. Beating hearts of Pleuronectes platessa were perfused by an aerated medium containing 0.1% collagenase and 0.1% hyaluronidase. After 60 min, the hearts were quite soft and easy to dissociate. The cardiac muscle cells were then collected on Nucleopore membranes, and their surface structure was revealed by scanning electron microscopy.
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Cellular Cardiology Research Group, University of Bergen, Norway.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D005412:Flatfishes / Q000033:anatomy & histology*; D008855:Microscopy, Electron, Scanning; D009206:Myocardium / Q000166:cytology*