Quantitative analysis of sterols in serum by high-performance liquid chromatography. Application to the biochemical diagnosis of cerebrotendinous xanthomatosis.
A method for the simultaneous determination of 5 alpha-cholestan-3 beta-ol and cholesterol in serum by high-performance liquid chromatography was developed. After addition of internal standard (5 beta-cholestan-3 alpha-ol) and saponification with ethanolic potassium hydroxide, the sterols were converted into their benzoyl derivatives, which were subjected to reversed-phase liquid chromatography with ultraviolet detection at 228 nm. Only 0.1 ml of serum was needed to give a reproducible result. This method has been used for the biochemical diagnosis of cerebrotendinous xanthomatosis, a hereditary disorder of cholesterol metabolism.
Kasama
T
T
Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.
Byun
D S
DS
Seyama
Y
Y
D001565:Benzoates; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet; D013261:Sterols / Q000097:blood*; D014973:Xanthomatosis / Q000097:blood / Q000175:diagnosis*
D001565:Benzoates; D007202:Indicators and Reagents; D013261:Sterols; C013409:benzoyl chloride