Nucleotide sequences of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.
Two genes, acyI and acyII, for distinct cephalosporin acylases from Pseudomonas sp. strain SE83 (A. Matsuda, K. Matsuyama, K. Yamamoto, S. Ichikawa, and K.I. Komatsu, J. Bacteriol. 169:5815-5820, 1987) were sequenced. Each sequence contained a single open reading frame for two nonidentical subunits, predicting a common precursor. Some homologies at the amino acid level were found between the acyII-encoded enzyme, but not the acyI-encoded one, and other related acylases.
Matsuda
A
A
Pharmaceutical Research and Development Department, Asahi Chemical Industry Co., Ltd., Fuji, Japan.
Toma
K
K
Komatsu
K
K
D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010399:Penicillin Amidase / Q000235:genetics*; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012984:Software
D004269:DNA, Bacterial; D000581:Amidohydrolases; D010399:Penicillin Amidase