Poly(styrene-divinylbenzene)-based strong anion-exchange packing material for high-performance liquid chromatography of proteins.
An adsorbed polyethyleneimine coating was applied to sulfonated macroporous, microparticulate poly(styrene-divinylbenzene). The chemical, physical and chromatographic properties of the resulting strong anion-exchange packing material were thoroughly characterized. The dynamic load capacity of the experimental packing was comparable to that of large-pore diameter silica. Good recoveries of protein mass and enzyme activity were achieved. The new column withstood a variety of cleaning procedures and prolonged exposure to aqueous base. The retention times on the polystyrene-based column were similar to those on a silica and a commercial, polymeric, strong anion-exchange column. Chromatographic resolution of the new packing material was equal or superior to that provided by the other two packings.
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Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
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Regnier
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D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins / Q000378:metabolism; D007202:Indicators and Reagents; D011137:Polystyrenes / Q000032:analysis*; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine
D006454:Hemoglobins; D007202:Indicators and Reagents; D011137:Polystyrenes; D011506:Proteins; D012710:Serum Albumin, Bovine; C003771:Amberlite XAD-2 resin