title: Concentrations of high density lipoproteins vary among follicular sizes in the bovine.
abstract: This study compared lipoprotein phospholipid, cholesterol, protein, and glycosaminoglycans in the lipoprotein fraction among follicular fluid pools derived from 2633 small (less than 6 mm), 1702 medium (6 to 10 mm), and 491 large (11 to 20 mm) bovine follicles subdivided by estradiol-17 beta concentrations. High density lipoproteins were the only lipoprotein class found in follicular fluid when apolipoprotein profile of the less than 1.21 g/ml density fraction was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and silver staining. Within a follicular size class, concentrations of lipoprotein constituents did not change among follicular fluid pools with varying estradiol-17 beta concentrations. As follicular size increased, follicular fluid lipoprotein phospholipid, cholesterol, and protein significantly increased. The percentage of total follicular fluid glycosaminoglycans in the less than 1.21 g/ml density fraction was highest for small follicles but averaged less than 5% for all follicular size classes. Therefore, bovine follicular fluid contains only high density lipoproteins and concentrations increased with follicular maturation. That increase in high density lipoprotein could be a potentially important source of cholesterol for progesterone production after the LH surge.
authors: Department of Dairy Science, University of Wisconsin, Madison 53706.
authors: S A
authors: SA
authors: Brantmeier
authors: R R
authors: RR
authors: Grummer
authors: R L
authors: RL
authors: Ax
mesh_terms: D000818:Animals; D002417:Cattle / Q000378:metabolism*; D008076:Cholesterol, HDL / Q000032:analysis; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis*; D008075:Lipoproteins, HDL / Q000032:analysis*; D006080:Ovarian Follicle / Q000032:analysis* / Q000166:cytology; D010743:Phospholipids / Q000032:analysis*
chemical_list: D008076:Cholesterol, HDL; D006025:Glycosaminoglycans; D008075:Lipoproteins, HDL; D010743:Phospholipids