title: Measurement of acetylcholinesterase in erythrocytes in the field.
abstract: We describe here a field method we developed for colorimetry of erythrocytic acetylcholinesterase (EC 3.1.1.7) in capillary blood samples. Three stable, premixed assay reagents and de-ionized water (but no centrifuge or balance) are required. This method, adapted for a microplate format, is essentially that of Ellman et al. (Biochem Pharmacol 1961;7:88-95) as modified by George and Abernethy (Clin Chem 1983;29:365-8). Assays were quantified and corrected for hematocrit by using a battery-powered colorimeter with a silicon carbide (blue) light-emitting-diode source. Advantages over existing field methods include better portability, ruggedness, greater precision, and lower cost per sample.
authors: Department of Internal Medicine, University of Cincinnati Medical Center, OH 45267-0585.
authors: R A
authors: RA
authors: Magnotti
authors: J P
authors: JP
authors: Eberly
authors: D E
authors: DE
authors: Quarm
authors: R S
authors: RS
authors: McConnell
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood*; D000293:Adolescent; D000328:Adult; D003124:Colorimetry; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D007306:Insecticides / Q000506:poisoning; D008297:Male; D008722:Methods; D009943:Organophosphorus Compounds; D011933:Reagent Kits, Diagnostic
chemical_list: D007306:Insecticides; D009943:Organophosphorus Compounds; D011933:Reagent Kits, Diagnostic; D000110:Acetylcholinesterase