title: Improvement of chemical analysis of antibiotics. XII. Simultaneous analysis of seven tetracyclines in honey.
abstract: An analytical system for the simultaneous determination of residual oxytetracycline, tetracycline, chlortetracycline, doxycycline, methacycline, demethylchlortetracycline and minocycline in honey has been established by a combination of simple thin-layer chromatographic (TLC) and precise high-performance liquid chromatographic (HPLC) methods. In this system, screening by TLC can detect tetracyclines (TCs) at a level of 0.1 ppm in honey without the need for special equipment, and the quantitative method by HPLC can determine TCs with good recovery (83.7-99.6%) and coefficient variation (0.9-4.3%).
authors: Aichi Prefectural Institute of Public Health, Nagoya, Japan.
authors: H
authors: H
authors: Oka
authors: Y
authors: Y
authors: Ikai
authors: N
authors: N
authors: Kawamura
authors: K
authors: K
authors: Uno
authors: M
authors: M
authors: Yamada
authors: K
authors: K
authors: Harada
authors: M
authors: M
authors: Suzuki
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004350:Drug Residues / Q000032:analysis; D006722:Honey / Q000032:analysis*; D007202:Indicators and Reagents; D013754:Tetracyclines / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D013754:Tetracyclines