The effects of ischaemia, lysophosphatidylcholine and palmitoylcarnitine on rat heart phospholipase A2 activity.
Phospholipase A2 activity was studied in the isolated rat heart following coronary artery ligation. In both the homogenate and mitochondrial fractions phospholipase A2 activity was significantly depressed at 20 min post ligation in the ischaemic region only. This is at a time of peak lysophospholipid concentration and severity of arrhythmias. No such depression of activity was seen in a crude sarcolemmal fraction, possibly due to washout of inhibitory factors during isolation. Lysophosphatidylcholine and palmitoylcarnitine, two amphiphiles known to accumulate during ischaemia, were both shown to be capable of inhibiting phospholipase A2. It is suggested that lysophospholipid and palmitoylcarnitine accumulation during ischaemia may contribute to the depression of phospholipase A2 activity seen and that the decreased metabolism of lysophospholipids may be of more importance in their accumulation than increased production by phospholipase A2.
Bentham
J M
JM
Pharmacology Group School of Pharmacy and Pharmacology, University of Bath, Claverton Down, U.K.
Higgins
A J
AJ
Woodward
B
B
D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives*; D003327:Coronary Disease / Q000378:metabolism*; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010172:Palmitoylcarnitine / Q000378:metabolism / Q000494:pharmacology*; D010477:Perfusion; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors* / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains
D008244:Lysophosphatidylcholines; D010172:Palmitoylcarnitine; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002331:Carnitine