Carboxypeptidase digestion in the presence of detergents.
The detergents, sodium dodecyl sulfate (SDS), lauryl glutamate (LG), and octyl-(polydisperse)oligooxyethylene (Octyl-POE), were tested as to their effects on the activities of carboxypeptidases A, B, and P. In general, Octyl-POE showed little inhibition and SDS showed the strongest inhibition. Carboxypeptidase B was only slightly inhibited by SDS. The inhibitory effect of SDS on these enzyme activities depended not on its concentration but on its absolute amount. For a constant amount of SDS, the activities of carboxypeptidases A and B remained almost constant with increasing reaction volume. Commercial carboxypeptidase B is usually contaminated by carboxypeptidase A. With the addition of SDS, almost only carboxypeptidase B activity was detected.
Kamo
M
M
Department of Chemistry, Faculty of Science, Science University of Tokyo, Chiba.
Tsugita
A
A
D002268:Carboxypeptidases / Q000037:antagonists & inhibitors*; D003902:Detergents / Q000494:pharmacology*; D007700:Kinetics; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D002268:Carboxypeptidases