Column purification of adenovirus cores.
A simple and efficient technique for purifying adenoviral chromatin (nucleoprotein cores) with Sephacryl S-1000 is described. This method is significantly faster than previous methods and gives a higher degree of purity with an increased recovery of the nucleoprotein. In addition, the structural integrity of the cores is maintained.
Cupo
J F
JF
Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Rezanka
L J
LJ
Harpst
J A
JA
D000180:Acrylic Resins; D000260:Adenoviruses, Human / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D014758:Viral Core Proteins / Q000302:isolation & purification*
D000180:Acrylic Resins; C037239:Sephacryl Superfine; D014758:Viral Core Proteins