Sensitive method for carbohydrate composition analysis of glycoproteins by high-performance liquid chromatography.
A new high-performance liquid chromatographic system has been developed for separating completely the neutral alditols and aminoalditols derived from sugars in complex carbohydrates. In combination with reduction by NaB3H4, this system affords a very sensitive and rapid method of monosaccharide composition analysis of glycoproteins and glycolipids.
Takeuchi
M
M
Department of Biochemistry, University of Tokyo, Japan.
Takasaki
S
S
Inoue
N
N
Kobata
A
A
D000107:Acetylation; D002241:Carbohydrates / Q000032:analysis*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004590:Electrophoresis, Paper; D004921:Erythropoietin / Q000032:analysis / Q000096:biosynthesis; D006023:Glycoproteins / Q000032:analysis*; D006868:Hydrolysis; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D013402:Sugar Alcohols / Q000032:analysis
D002241:Carbohydrates; D006023:Glycoproteins; D011994:Recombinant Proteins; D013402:Sugar Alcohols; D004921:Erythropoietin