title: Conversion of peanut trypsin-chymotrypsin inhibitor B-III to a chymotrypsin inhibitor by deimination of the P1 arginine residues in two reactive sites.
abstract: The deimination of the arginine residues in peanut trypsin-chymotrypsin inhibitor B-III caused the disappearance of its trypsin-inhibitory activity. Peanut protease inhibitor B-III was incubated with peptidylarginine deiminase, resulting in the conversion of 2.5 mol of arginine to citrulline and in the loss of its trypsin-inhibitory activity. However, the ability of the deiminated inhibitor to inhibit chymotrypsin was as strong as before. Structural analysis of the deiminated B-III indicated that the P1 arginine residues at both reactive sites, Arg(10) and Arg(38), were completely modified to citrulline by the action of peptidylarginine deiminase, and that the Arg(60) in the C-terminal region of B-III was partially deiminated. These residues seem to be exposed on the surface of the molecule. The P1' arginine residue at the first reactive site, Arg(11), was not deiminated at all.
authors: Department of Chemistry, Osaka University College of Science.
authors: T
authors: T
authors: Kurokawa
authors: S
authors: S
authors: Hara
authors: H
authors: H
authors: Takahara
authors: K
authors: K
authors: Sugawara
authors: T
authors: T
authors: Ikenaka
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000378:metabolism*; D001665:Binding Sites; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D002956:Citrulline / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006867:Hydrolases / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014361:Trypsin Inhibitors / Q000032:analysis / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014361:Trypsin Inhibitors; C037954:trypsin-chymotrypsin inhibitor B-III, peanut; D002956:Citrulline; D001120:Arginine; D006867:Hydrolases; D002918:Chymotrypsin; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases