Crystallization of a DNA duplex 15-mer containing unpaired bases: d(CGCGAAATTTACGCG).
Crystals of an almost self-complementary DNA 15-mer d(CGCGAAATTTACGCG) have been grown by the vapor diffusion technique at 4 degrees C. The space group is I222 with a = 37.3 A, b = 54.6 A and c = 104.8 A. Solution studies showed that the 15-mer forms a duplex with the extra adenine residue unpaired: (sequence; see text) Crystals are stable at 4 degrees C and are suitable for medium-resolution structural studies.
Miller
M
M
Center for Chemical Physics, National Bureau of Standards, Gaithersburg, MD 20899.
Wlodawer
A
A
Appella
E
E
Sussman
J L
JL
D001483:Base Sequence; D003460:Crystallization; D011089:Polydeoxyribonucleotides
D011089:Polydeoxyribonucleotides