title: Levels of prodynorphin mRNA in rat dentate gyrus are decreased during hippocampal kindling.
abstract: The effect of hippocampal kindling on the levels of prodynorphin mRNA in rat hippocampus was examined by in situ hybridization using a synthetic oligonucleotide probe. Cryostat tissue sections were hybridised with a 32P-labelled 100 mer DNA probe complementary to the coding region of rat prodynorphin mRNA, and exposed to X-ray film. In rats exhibiting stage 4 seizures, the levels of prodynorphin mRNA in the dentate gyrus were dramatically reduced compared to control animals. This suggests that the development of kindling is accompanied by a reduction in the rate of synthesis of peptides derived from prodynorphin.
authors: Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, F.R.G.
authors: B J
authors: BJ
authors: Morris
authors: M E
authors: ME
authors: Moneta
authors: G
authors: G
authors: ten Bruggencate
authors: V
authors: V
authors: Höllt
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D004745:Enkephalins / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism* / Q000503:physiopathology; D007696:Kindling, Neurologic; D008297:Male; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; C033660:preproenkephalin