Determination of aspoxicillin (TA-058) by high-performance liquid chromatography. Stability at different temperatures.
A rapid and sensitive HPLC-method has been developed for the determination of serum concentrations of aspoxicillin (TA-058), a new semisynthetic beta-lactam antibiotic. Aspoxicillin was chromatographed with a phosphate buffer/methanol (92:8 v/v) mobile phase and a C-18 reversed phase column and was detected at a wavelength of 220 nm. The stability of aspoxicillin in serum and buffer at different temperatures was studied over a time period of 3 months. Furthermore, the degradation of aspoxicillin versus piperacillin was determined in serum and buffer at 37 degrees C. Aspoxicillin remains stable only at -70 degrees C whereas degradation has been observed at -20 degrees C and 4 degrees C. At 37 degrees C, 20% of aspoxicillin is degraded in serum within 24 h whereas piperacillin is completely degraded under the same conditions.
Knöller
J
J
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, AG Infektabwehrmechanismen, Ruhr Universität, Bochum.
Schönfeld
W
W
Bremm
K D
KD
König
W
W
D000658:Amoxicillin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010878:Piperacillin / Q000493:pharmacokinetics; D013696:Temperature
C037485:aspoxicillin; D000658:Amoxicillin; D010878:Piperacillin