title: Specific binding of B-CLL cell-derived chemokinetic inhibitory factor (CIF) to human polymorphonuclear leukocytes.
abstract: The chemokinetic inhibitory factor (CIF) is a recently described B-cell derived lymphokine that mediates a chemokinetic inhibitory effect on human polymorphonuclear leukocyte (PMN) migration. In the present report the interaction of CIF with the neutrophil plasma membrane was studied. Normal human peripheral blood neutrophils and purified neutrophil plasma membranes selectively removed biologic activity from CIF-containing concentrates obtained during the purification procedure from conditioned medium. Removal was obtained at both 4 degrees C and 37 degrees C. Furthermore, HL-60 cells treated with dimethyl sulfoxide removed CIF activity (granulocyte-like cells) but HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (macrophage-like cells) did not. Purified human blood monocytes, cells from the macrophage-like U-937 cell line and cells from the basophilic leukemia cell line KU-812 did not remove CIF. These studies suggest that neutrophils express specific binding sites for CIF-activity.
authors: Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
authors: A
authors: A
authors: Siegbahn
authors: R C
authors: RC
authors: Garcia
authors: K
authors: K
authors: Kishi
authors: K
authors: K
authors: Nilsson
authors: P
authors: P
authors: Venge
mesh_terms: D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors; D005944:Glucosamine / Q000494:pharmacology; D006595:Hexosamines / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008222:Lymphokines / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D002630:Chemotactic Factors; D006595:Hexosamines; D008222:Lymphokines; C034397:chemotactic inhibitory factor; D005944:Glucosamine; C002022:N-acetylmannosamine