On the choice of laser dyes for use in exciting tyrosine fluorescence decays.
The choice of laser dyes for exciting tyrosine fluorescence using synchronously pumped cavity-dumped dye laser systems is discussed. Rhodamine 560 was found to be optimal for a system based on an argon-ion pumping laser, whereas rhodamine 575 was preferred using a frequency-doubled Nd:YAG laser. Modifications of our fluorescence decay instrument to permit rejection of multiphoton events using a microchannel plate photomultiplier are described. An example of a four-component resolution of tyrosine decays illustrates the dramatic resolution capabilities attainable.
Libertini
L J
LJ
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Small
E W
EW
D007834:Lasers; D012235:Rhodamines; D013050:Spectrometry, Fluorescence; D014443:Tyrosine; D014966:Xanthenes
D012235:Rhodamines; D014966:Xanthenes; D014443:Tyrosine