A protocol and guide for the in vivo rat hepatocyte DNA-repair assay.
The in vivo rat hepatocyte DNA-repair assay is a valuable tool in assessing the genotoxic activity of chemical agents. An advantage of the system is that it reflects the complex patterns of uptake, distribution, metabolism, detoxification and excretion that actually occur in the whole animal. This article provides a typical procedure and guidelines for conducting the rat in vivo hepatocyte DNA-repair assay.
Butterworth
B E
BE
Chemical Industry Institute of Toxicology, Research Triangle Park, NC 27709.
Ashby
J
J
Bermudez
E
E
Casciano
D
D
Mirsalis
J
J
Probst
G
G
Williams
G
G
D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D009152:Mutagenicity Tests / Q000592:standards*; D009153:Mutagens / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity; D010477:Perfusion; D051381:Rats; D012107:Research Design
D009153:Mutagens