Porphyrin test as an alternative to benzidine test for detecting cytochromes in catalase-negative gram-positive cocci.
A total of 66 strains of gram-positive cocci, including 21 catalase-negative members of the family Streptococcaceae and strains of Stomatococcus mucilaginosus, were investigated for the ability to produce porphobilinogen and porphyrin from delta-aminolevulinic acid as an alternative to the benzidine test for detecting the presence of cytochromes. Production of porphobilinogen correlated 100% with membership in the family Micrococcaceae.
Wong
J D
JD
Microbial Diseases Laboratory, California State Department of Health Services, Berkeley 94704.
D000622:Aminolevulinic Acid / Q000378:metabolism; D002374:Catalase / Q000096:biosynthesis; D003580:Cytochromes / Q000032:analysis*; D006094:Gram-Positive Bacteria / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D008835:Micrococcaceae / Q000032:analysis / Q000145:classification / Q000378:metabolism; D011162:Porphobilinogen / Q000096:biosynthesis*; D011166:Porphyrins / Q000096:biosynthesis*; D013289:Streptococcaceae / Q000032:analysis / Q000145:classification / Q000378:metabolism
D003580:Cytochromes; D011166:Porphyrins; D011162:Porphobilinogen; D000622:Aminolevulinic Acid; D002374:Catalase