Evaluation of the biocompatibility of a Ni-Cr-Mo dental alloy with human gingival explant culture in vitro: morphological study, immunodetection of fibronectin, and collagen production.
This study was undertaken to evaluate the biocompatibility of a Ni-Cr-Mo dental casting alloy by an in vitro explant culture method. Through ultrastructural features, the immunolabelling of fibronectin, and the immunodetection of type I and III collagen production, we investigated the behaviour of gingival cells (both fibroblasts and epithelial cells) in contact with the alloy in comparison with control cultures. Our results indicate that cultured gingival cells present a well preserved ultrastructure and synthesized fibronectin (the main glycoprotein involved in adhesion to substrates). The alteration of collagen production concerned only type III collagen which decreased significantly in the cultures on the dental alloy.
Exbrayat
P
P
Laboratoire d'Histophysiologie et de Pathologie des Tissus Dentaires, U.A. CNRS 819, Faculté d'Odontologie de Lyon, France.
Couble
M L
ML
Magloire
H
H
Hartman
D J
DJ
D002478:Cells, Cultured; D002857:Chromium / Q000633:toxicity; D003094:Collagen / Q000096:biosynthesis*; D003722:Dental Alloys; D004577:Electron Probe Microanalysis; D005353:Fibronectins / Q000032:analysis*; D005881:Gingiva / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D008854:Microscopy, Electron; D008982:Molybdenum / Q000633:toxicity; D009532:Nickel / Q000633:toxicity; D011863:Radioimmunoassay
D003722:Dental Alloys; D005353:Fibronectins; D002857:Chromium; D009532:Nickel; D008982:Molybdenum; D003094:Collagen