Preparation of cell envelopes of large numbers of individual bacterial strains with the use of an automatic cell disruptor.
Analysis of the cell envelopes of large numbers of bacterial strains is used for the epidemiological and taxonomic investigation of clinical, veterinarian, and ecological isolates. Isolation of cell envelopes requires lysis of the bacteria. We developed an apparatus to disrupt bacterial cells of 200 different isolates in suspension by ultrasonication automatically. It is composed of modified standard laboratory equipment (fraction collector, cooling unit, pump), a standard ultrasonifier, and a newly designed control unit, which includes a sampler. This apparatus was applied to the analysis of cell envelope proteins of 96 Haemophilus influenzae strains on sodium dodecyl sulfate-polyacrylamide gel electrophoresis within 3 days after the first culture.
Van Alphen
L
L
Department of Medical Microbiology, Academic Medical Centre, Amsterdam, The Netherlands.
Romijn
C
C
Brandt
H
H
Geelen
L
L
Zanen
H C
HC
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation; D004591:Electrophoresis, Polyacrylamide Gel; D006190:Haemophilus / Q000032:analysis
D001425:Bacterial Outer Membrane Proteins