Limited nucleosome migration can completely randomize DNA repair patches in intact human cells.
Following irradiation of human cells with ultraviolet light, DNA repair patches are initially inserted near the 5' and 3' ends of nucleosome core DNA leaving a "gap" in repair synthesis (of approximately 50 bases) near the center of the core DNA. With time, however, these same repair patches become randomized, apparently by nucleosome migration. We have developed both an analytical expression and a computer algorithm (which simulates nucleosome migration along DNA) to determine the average distance nucleosomes must migrate to change the initial, non-uniform distribution of repair patches in nucleosomes to a random distribution. Both of these methods yielded the same result: nucleosomes must migrate an average of about 50 base-pairs in either direction to produce the randomization observed.
Arnold
G E
GE
Biochemistry/Biophysics Program, Washington State University, Pullman 99164-4660.
Dunker
A K
AK
Smerdon
M J
MJ
D002465:Cell Movement / Q000528:radiation effects; D003198:Computer Simulation; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects*; D006801:Humans; D008954:Models, Biological; D009707:Nucleosomes / Q000528:radiation effects*; D012984:Software; D014466:Ultraviolet Rays
D009707:Nucleosomes; D004247:DNA