A new tetracycline antibiotic from a Dactylosporangium species. Fermentation, isolation and structure elucidation.
An actinomycete identified as a Dactylosporangium sp. produces a new tetracycline, 4a-hydroxy-8-methoxychlortetracycline (Sch 34164). The addition of magnesium ions to complex fermentation media increased the antibiotic titers. Sch 34164 was isolated by solvent extraction and Sephadex G-25 column chromatography. The novel structure was proposed based on spectroscopic analysis. The shift of C-4a (35 to 77 ppm) and C-8 (140 to 163 ppm) in the 13C NMR as compared to chlortetracycline was indicative of the novel hydroxyl and methoxy substituents, respectively.
Patel
M
M
Department of Microbial Products, Schering Corporation, Bloomfield, NJ 07003.
Gullo
V P
VP
Hegde
V R
VR
Horan
A C
AC
Marquez
J A
JA
Vaughan
R
R
Puar
M S
MS
Miller
G H
GH
D000192:Actinomycetales / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002751:Chlortetracycline / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy
C054353:dactylocyclinone; D002751:Chlortetracycline