RecA-like activity in mammalian cell extracts of different origin.
The occurrence of a RecA-like activity similar to the one detected in the fibroblast cell line GM1492 derived from a patient suffering from the autosomal recessive disease Bloom's syndrome has been investigated in cell extracts of different origin. The formation of D-loop containing joint molecules from phi X174 RFI DNA and fragments of phi X174 single-stranded DNA by partially purified extracts was measured by a filter binding assay. The RecA-like activity, dependent on ATP and Mg2+, was detected at an elevated level only in the human and rodent cell lines, GM1492 and CHO respectively. The level of activity in DNA-cellulose-purified cell extracts from these cell lines was 4-7-fold higher compared to normal human fibroblasts. Low levels of activity were also detected in extracts from two additional Bloom's syndrome fibroblast cell lines, Fanconi's anemia fibroblasts, virus- (Epstein-Barr virus, Simian virus 40) transformed human cells and human placenta. Cell extracts from rat testis, spleen and calf thymus were also of low activity.
Kenne
K
K
Department of Medical Cell Genetics, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.
Ljungquist
S
S
D000741:Anemia, Aplastic / Q000235:genetics*; D000818:Animals; D001816:Bloom Syndrome / Q000235:genetics*; D002417:Cattle; D002460:Cell Line; D002461:Cell Line, Transformed; D004260:DNA Repair; D005199:Fanconi Anemia / Q000235:genetics*; D006801:Humans; D051381:Rats; D011938:Rec A Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic; D014018:Tissue Distribution
D011938:Rec A Recombinases