title: Evidence for a novel FMRFamide-related heptapeptide in the pulmonate snail Siphonaria pectinata.
abstract: Extracts of whole false limpets (Siphonaria pectinata) were analysed to determine their complement of FMRFamide-related peptides. As in other pulmonates, FMRFamide itself was found to account for only a portion of the immunoreactivity; the largest immunoreactive peptide peak eluted during HPLC under acidic conditions at the same position as a peak also found in other pulmonates. This major peak was resolved into two components by HPLC at neutral pH, and one component was identified as the heptapeptide amide, GDPFLRFamide, previously described from Lymnaea. The amino acid composition of the second component indicates that it is also a heptapeptide, but that it has two Asx (aspartic acid or asparaginyl) residues instead of the one found in the previously identified pulmonate heptapeptides.
authors: C. V. Whitney Laboratory, University of Florida, St. Augustine 32086.
authors: D A
authors: DA
authors: Price
authors: C G
authors: CG
authors: Cobb
authors: K E
authors: KE
authors: Doble
authors: J K
authors: JK
authors: Kline
authors: M J
authors: MJ
authors: Greenberg
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D019835:FMRFamide; D008195:Lymnaea / Q000378:metabolism; D009479:Neuropeptides / Q000032:analysis*; D011863:Radioimmunoassay; D012908:Snails / Q000378:metabolism*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000596:Amino Acids; D009479:Neuropeptides; D012997:Solvents; D019835:FMRFamide