Effects of free fatty acids, lysophosphatides and phospholipase treatment on lipid peroxidation of myocardial homogenates and membrane fractions.
The effects of various free fatty acids, lysophosphatides and phospholipase treatments on the enzymatic and the non-enzymatic lipid peroxidation capacities in the heart homogenates and subcellular fractions were studied. The results showed a dose related inhibition of both the enzymatic and non-enzymatic lipid peroxidation with free fatty acids. A significant inhibition occurred as early as at the concentration of 25-50 microM of several fatty acids both in homogenates and in organelle fractions. In general, the inhibition was greatest with cis-unsaturated, long-chain fatty acids. The inhibition was also induced by the pretreatment of the homogenates with phospholipase A2 but not with phospholipase C. The lysophosphatidyl cholines (16:0 and 18:1) had a stimulatory effect on the enzymatic lipid peroxidation capacity at the physiological concentrations. The results show that the stimulatory/inhibitory effect of various lipid amphiphiles on lipid peroxidation is strongly structure linked and the mitochondrial fraction is the most susceptible to the injury induced by lipid amphiphiles.
Kihlström
M
M
Department of Cell Biology, University of Jyväskylä, Finland.
Marjomäki
V
V
Salminen
A
A
D000818:Animals; D003327:Coronary Disease / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008055:Lipids / Q000494:pharmacology*; D008246:Lysophospholipids / Q000494:pharmacology; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D010740:Phospholipases / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D005230:Fatty Acids, Nonesterified; D008054:Lipid Peroxides; D008055:Lipids; D008246:Lysophospholipids; D008563:Membrane Lipids; D010740:Phospholipases