title: A versatile procedure for the radioiodination of proteins and labeling reagents.
abstract: For tracer or analytical studies it is often useful to label proteins by direct iodination or by reacting them with an iodinated reagent. A simple iodination technique with hydrogen peroxide is described for use with either carrier-free or low-specific-activity iodine. The method introduces less oxidative damage to proteins than any other procedure tested, yet the efficiency of labeling approaches that offered by the chloramine T or Iodogen methods. The method has been applied to the facile and inexpensive preparation of the iodinated Bolton-Hunter reagent. This peroxide iodination procedure should be particularly useful for labeling proteins or peptides for structural investigations or for immunoassays.
authors: Department of Fine Structure Research, Boston Biomedical Research Institute, MA 02114.
authors: P F
authors: PF
authors: Davison
mesh_terms: D002700:Chloramines; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D011506:Proteins; D011854:Radiochemistry; D013388:Succinimides; D014105:Tosyl Compounds; D014508:Urea / Q000031:analogs & derivatives
chemical_list: D002700:Chloramines; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011506:Proteins; D013388:Succinimides; D014105:Tosyl Compounds; C016300:chloramine-T; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; C022581:Bolton-Hunter reagent; D014508:Urea; D006861:Hydrogen Peroxide