Organ culture of chicken cecum: morphologic and physiologic observations after 24 and 48 h of culture.
Cecum from chickens, 4 wk old, can best be maintained for 24 h in a serum-free organ culture system using Trowell T8 medium agar sheet at 25 degrees C. As determined by light microscopy as well as scanning and transmission electron microscopy, mucosal architecture involving classic ultrastructure of chicken cecal mucosa was preserved. Protein content of cecal explants did not change up to 48 h of culture. DNA content of cecal explants did not change up to 24 h of culture but decreased significantly to two-thirds of control in 48 h of culture. Based on the morphologic and physiologic findings, it became evident that this organ culture system using Trowell T8 medium at 25 degrees C can be successfully used as an in vitro experimental model for as long as 24 h. The organ culture system could be a useful tool, from the structural integrity of ceca observed in this study, in investigating mucosal function and mucosal response to drugs, carcinogens, trophic factors, and pathogens.
Sasai
K
K
Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
Arakawa
A
A
D000818:Animals; D002432:Cecum / Q000166:cytology* / Q000648:ultrastructure; D002645:Chickens; D004261:DNA Replication; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013936:Thymidine