Decoloration and solubilization of plant tissue prior to determination of 3H, 14C, and 35S by liquid scintillation.
A method is described for the decoloration and partial solubilization of plant tissue with 2% sodium hypochlorite. Following treatment of the digest with ammonia, the samples are suitable for the determination of 3H, 14C, and 35S by liquid scintillation counting. The color quenching is negligible and counting efficiencies are high: 30-40% for 3H and 90-95% for 14C.
Smith
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Department of Botany, Ohio University, Athens 45701.
Lang
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D002250:Carbon Radioisotopes / Q000032:analysis; D010858:Pigmentation; D010944:Plants / Q000032:analysis*; D012588:Scintillation Counting / Q000379:methods*; D012973:Sodium Hypochlorite; D012995:Solubility; D013462:Sulfur Radioisotopes / Q000032:analysis; D014316:Tritium / Q000032:analysis
D002250:Carbon Radioisotopes; D013462:Sulfur Radioisotopes; D014316:Tritium; D012973:Sodium Hypochlorite