The turnover of radiolabeled nuclear proteins in rats exposed to environmental and chemical stress.
Exposure to a 12 h light/12 h dark (L/D) cycle for 1 month, followed by reversal to a 12 h D/12 h L (D/L) cycle stimulated within 18 h the incorporation of [3H]leucine and [32P]orthophosphoric acid into new proteins (130-25 kDa) in the G0 phase of the cell cycle of the non-regenerating and regenerating rat liver as observed in two-dimensional gel autoradiograms. Six additional proteins from the rat submaxillary gland (130-20 kDa) revealed labeling with 32P within 3 h following combined administration of isoproterenol and sodium arsenite. Labeling disappeared within 7 days for all stressed proteins.
Pipkin
J L
JL
Department of Health and Human Services, Food and Drug Administration, Jefferson, AR 72079.
Anson
J F
JF
Hinson
W G
WG
Duffy
P H
PH
Burns
E R
ER
Casciano
D A
DA
D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D018053:Arsenites; D002453:Cell Cycle / Q000187:drug effects; D007545:Isoproterenol / Q000633:toxicity*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008115:Liver Regeneration; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D051381:Rats; D017670:Sodium Compounds; D013312:Stress, Physiological / Q000503:physiopathology*; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D014316:Tritium
D018053:Arsenites; D009687:Nuclear Proteins; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D017670:Sodium Compounds; D014316:Tritium; C017947:sodium arsenite; D007930:Leucine; D007545:Isoproterenol; D001151:Arsenic