Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-ammonium nitrate eluent. The effect of the mobile phase and the operating conditions.
The reproducibility of capacity factors and relative capacity factors are compared as methods for recording retentions for the high-performance liquid chromatography of basic drugs on a silica column with methanol-aqueous ammonium nitrate as the eluent. The effects of changing the column temperature and the ionic strength, pH and proportion of organic modifier in the eluent on the retentions and selectivity have been studied. The results suggest that the mobile phase and operating conditions must be closely defined in order to obtain results of adequate reproducibility to develop a data base of retention values for interlaboratory comparisons and/or for the identification of basic drugs.
Smith
R M
RM
Department of Chemistry, University of Technology, Loughborough, Leics, U.K.
Hurdley
T G
TG
Gill
R
R
Osselton
M D
MD
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D009566:Nitrates; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013696:Temperature
D009566:Nitrates; D004364:Pharmaceutical Preparations; C006568:ammonium nitrate; D000432:Methanol