Antidiuretic factor from the nervous corpora cardiaca of the migratory locust: improvement of an existing in vitro bioassay.
Nervous lobes of the migratory locust corpora cardiaca contain an antidiuretic (AD) factor which increases the fluid reabsorption of isolated recta in a dose-dependent manner. Several extraction procedures were developed and compared. The very satisfactory thermostability of the AD factor was demonstrated. By using a succession of different incubating media on the same preparation, we improved our bioassay. The developed variant is faster and more sensitive than the existing test and saves time and biological material.
Fournier
B
B
Laboratoire de Neuroendocrinologie, UA CNRS 1138, Université de Bordeaux I, Talence, France.
Herault
J P
JP
Proux
J
J
D000818:Animals; D001681:Biological Assay; D004231:Diuresis; D006110:Grasshoppers / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008970:Molecular Weight; D009490:Neurosecretory Systems / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*
D014020:Tissue Extracts