Quantitative evaluation of colonizing ability of Vibrio cholerae O1.
A new method to evaluate the adhesive ability of Vibrio cholerae O1 was proposed. Broth cultured V. cholerae O1 and a piece of formalin-fixed rabbit intestinal wall were incubated together in KRT buffer and the number of adhered organisms was counted under a scanning electron microscope. This method was much less laborious than other methods that have been used so far, and most significantly, constant results were obtained in repeated experiments. The adhesive properties of toxigenic V. cholerae O1 evaluated by this method correlated well with its observed experimental pathogenicity.
Nakasone
N
N
Department of Bacteriology, University of the Ryukyus School of Medicine, Okinawa.
Iwanaga
M
M
D000818:Animals; D001422:Bacterial Adhesion; D003470:Culture Media; D046508:Culture Techniques; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D014734:Vibrio cholerae / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure
D003470:Culture Media