High-performance liquid chromatography of amino acids, peptides and proteins. LXXIV. Separation of heparin-binding growth factors by reversed-phase high-performance liquid chromatography.
The separation of several immunologically related forms of the bovine brain basic heparin-binding growth factor by reversed-phase high-performance liquid chromatography is described. With Bakerbond C4 reversed-phase columns, it is possible to resolve the 0.8-1.0 M sodium chloride and the 1.0-1.3 M sodium chloride components from the preceding heparin-Sepharose affinity chromatographic step in the purification procedure for these polypeptide mitogens into multiple active forms, all of which exhibit similar molecular weights and immunoreactivity with specific polyclonal antisera. Structural characterisation suggests that it is likely that these forms represent different stages in the post-translational processing of these polypeptide mitogens.
Hearn
M T
MT
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Guthridge
M
M
Bertolini
J
J
D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D016222:Fibroblast Growth Factor 2; D005347:Fibroblasts / Q000378:metabolism; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D006493:Heparin / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D013936:Thymidine / Q000378:metabolism
D000596:Amino Acids; D006133:Growth Substances; D008934:Mitogens; D010455:Peptides; D011506:Proteins; D016222:Fibroblast Growth Factor 2; D006493:Heparin; D013936:Thymidine