Mitogenic stimulation transiently increases the exchangeable mitochondrial calcium pool in rat thymocytes.
Exchangeable calcium pools were measured in rat thymocytes by 45Ca labelling and selective depletion of intracellular pools with oligomycin in the presence or absence of rotenone. The mitochondrial pool increased by 150% after 3 min of treatment with the mitogen concanavalin A, and decreased to zero 10 min after mitogen addition. No significant change in the ATP-dependent pool could be detected.
Lakin-Thomas
P L
PL
Department of Biochemistry, University of Cambridge, U.K.
Brand
M D
MD
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012402:Rotenone / Q000494:pharmacology; D013268:Stimulation, Chemical; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism*
D009840:Oligomycins; D012402:Rotenone; D003208:Concanavalin A; D000255:Adenosine Triphosphate; D002118:Calcium; D004121:Dimethyl Sulfoxide