title: Liquid chromatographic assay for the simultaneous determination of pyrazinamide and rifampicin in serum samples from patients with tuberculous meningitis.
abstract: A simple high-performance liquid chromatographic assay for the simultaneous determination of pyrazinamide and rifampicin in serum from patients with tuberculous meningitis is presented. The drugs and internal standard, p-acetamidobenzoic acid, were extracted from the acidified sample containing 2% ascorbic acid at pH 4.2 into dichloromethane-diethyl ether (2:3). The solvent extract was evaporated to dryness with the aid of nitrogen and the residue redissolved in methanol (75 microliters). The concentrate was analysed by a liquid chromatograph using a reversed-phase 30-microns C8 pre-column linked to a 5-microns C8 analytical column with a gradient solvent programme, which delivered 6% to 48% (v/v) acetonitrile in phosphate buffer (10 mM potassium dihydrogenphosphate, pH 3.5) in 10 min at 1.5 ml/min. The eluate was detected at 215 nm. Twelve patients with tuberculous meningitis were given daily chemotherapy, and their serum samples were assayed for pyrazinamide and rifampicin.
authors: Department of Medicine, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T.
authors: J
authors: J
authors: Woo
authors: C L
authors: CL
authors: Wong
authors: R
authors: R
authors: Teoh
authors: K
authors: K
authors: Chan
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011718:Pyrazinamide / Q000097:blood*; D012293:Rifampin / Q000097:blood*; D014390:Tuberculosis, Meningeal / Q000097:blood*
chemical_list: D011718:Pyrazinamide; D012293:Rifampin