Distribution of carbon tetrachloride-metabolite(s) to DNase I-sensitive and -resistant chromatin.
The distribution of carbon tetrachloride (CCl4) metabolite(s) to DNase I-sensitive and -resistant regions of mouse hepatic chromatin was determined following incubation of whole chromatin with [14C]CCl4 in the presence of hepatic microsomes and an NADPH-regenerating system. At 2 h of incubation, [14C]CCl4 metabolite(s) bound essentially to the same extent to DNase I-sensitive and -resistant chromatin regions. Binding of [14C]CCl4 metabolite(s) to DNase I-resistant regions, however, increased significantly over the DNase I-sensitive regions when incubation was prolonged to 4 h. This result suggests that CCl4 does not bind preferentially to DNase I-sensitive regions of chromatin.
Oruambo
I F
IF
Institute of Environmental Medicine, New York University Medical Center, NY 10016.
Van Duuren
B L
BL
D001711:Biotransformation; D002251:Carbon Tetrachloride / Q000378:metabolism* / Q000633:toxicity; D002843:Chromatin / Q000378:metabolism*; D004249:DNA Damage; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D006609:High Mobility Group Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000139:chemically induced*; D008862:Microsomes, Liver / Q000378:metabolism
D002843:Chromatin; D006609:High Mobility Group Proteins; D006657:Histones; D002251:Carbon Tetrachloride; D003850:Deoxyribonuclease I