title: Humoral immune response of foals to experimental infection with Rhodococcus equi.
abstract: Humoral immune response to Rhodococcus equi in experimentally infected foals was studied with the enzyme-linked immunosorbent assay (ELISA) method. Class-specific antibodies were measured by ELISA in the sera of foals after intratracheal or oral inoculation with R. equi ATCC 6939 or T 48 and in the lung washings of a foal after intratracheal inoculation or of normal horses. After intratracheal or oral inoculation with R. equi, serum antibodies were first detected in immunoglobulin G (IgG) followed by IgM and IgA classes, but significant levels of IgM and IgA developed only in the foal infected intratracheally with R. equi T 48. Only the foal infected intratracheally with T 48 developed pneumonia. Anti-R. equi IgG and IgA antibodies appeared in lung washings of the intratracheally infected foal. There were differences in the antibody response to R. equi among the intratracheally infected foals, the orally infected foal and the naturally infected foal. These results suggest that the humoral immune response to R. equi may be affected by the type of R. equi strain and the route and extent of R. equi exposure.
authors: Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Aomori, Japan.
authors: S
authors: S
authors: Takai
authors: S
authors: S
authors: Kawazu
authors: S
authors: S
authors: Tsubaki
mesh_terms: D000193:Actinomycetales Infections / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000032:analysis*; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D006734:Horse Diseases / Q000276:immunology / Q000382:microbiology*; D006736:Horses; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D012240:Rhodococcus / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M