Effect of vitamin A compounds on DNA biosynthesis in murine tumor models in vitro.
The effect of vitamin A compounds on the DNA biosynthesis inhibition of murine ascites tumor models such as P388 lymphocytic leukemia, L1210 lymphoid leukemia, sarcoma 180 and Ehrlich ascites carcinoma was found to be concentration- and time-dependent. The results of the experiments carried out in vitro for 4 h measured by the extent of 3H-thymidine incorporation into the DNA of these tumor cells permit us to conclude that (a) at the given concentration and time, the metabolic role of vitamin A compounds influences the regulation of processes leading to DNA biosynthesis inhibition; (b) the effect of vitamin A is uniform irrespective of tumor cells heterogeneity; (c) vitamin A does not affect transport of thymidine, and (d) the dose-dependent increase in the inhibition of these tumor cells by vitamin A is characterized by undifferentiated morphology showing uniformity in the given tumor cell population associated with a total lack of differentiation.
Satyamoorthy
K
K
Cancer Research Institute, Tata Memorial Center, Parel, Bombay, India.
Chitnis
M P
MP
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D003864:Depression, Chemical; D004224:Diterpenes; D007939:Leukemia L1210 / Q000188:drug therapy / Q000378:metabolism; D007941:Leukemia P388 / Q000188:drug therapy / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism; D012172:Retinaldehyde / Q000494:pharmacology / Q000627:therapeutic use*; D012176:Retinoids / Q000627:therapeutic use*; D000084562:Retinyl Esters; D012510:Sarcoma 180 / Q000188:drug therapy / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology / Q000627:therapeutic use*; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use*
D004224:Diterpenes; D012176:Retinoids; D000084562:Retinyl Esters; D014801:Vitamin A; C009166:retinol acetate; D014212:Tretinoin; D012172:Retinaldehyde