[Nuclear proteins--substrates for cAMP-dependent protein kinase].
The phosphorylation of nuclear proteins of porcine brain cAMP-dependent protein kinase was studied. Some nuclear proteins after extraction from the nuclei served as substrates for protein kinase. Lysine-rich histones H1, H2a and H2b were found to accept phosphate during chromatin phosphorylation by cAMP-dependent protein kinase. Phosphorylation of intact nuclei revealed that in such a system only histone H1 is a substrate for cAMP-dependent protein kinase. In the presence of DNA the histones are phosphorylated by cAMP-dependent protein kinase in a different manner. It was concluded that DNA can determine the accessibility of protein substrates for the catalytic subunit of cAMP-dependent protein kinase.
Nesterova
M V
MV
Glukhov
A I
AI
Aprikian
A G
AG
Severin
E S
ES
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine
D006657:Histones; D011494:Protein Kinases
Iadernye belki--substraty cAMP-zavisimoĭ proteinkinazy.