Surface affinity chromatographic separation of blood cells. VI. Capacity factors of human peripheral blood cells on poly(propylene glycol) bonded chromagel columns and their correlation with surface hydrophobicities of the gel beads and the cells.
The chromatographic behaviour of human peripheral blood cells was investigated on poly(propylene glycol) (PPG)-bonded Chromagel. The mobile phase was 0.09 M phosphate-buffered 2% (w/w) dextran T40 solution at the pH of the respective isoelectric points of human blood cells. The elution order of four kinds of blood cells from PPG-C3-Chromagel columns coincided with that of the delta log K values of these cells determined by the hydrophobic affinity partition method using Pluronic P84 as a hydrophobic ligand. The capacity factors of granulocytes and lymphocytes increased with increase in the delta log K values of PPG-C3-Chromagel beads. Hydrophobic interactions contributed to the retention of the four kinds of blood cells on PPG-bonded Chromagel columns.
Shibusawa
Y
Y
Division of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
Matsumoto
U
U
Takatori
M
M
D001773:Blood Cells; D002469:Cell Separation / Q000379:methods*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity; D005782:Gels; D006801:Humans; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011108:Polymers; D011409:Propylene Glycols
D005782:Gels; D011108:Polymers; D011409:Propylene Glycols; C012504:polypropylene glycol