title: Expression of the mouse P(1)450 gene during differentiation without foreign chemical stimulation.
abstract: Transcriptional activation of the P(1)450 and P(3)450 genes, mRNA accumulation, and induction of the P(1)450 and P(3)450 enzymes are known to be stimulated by foreign chemicals such as tetrachlorodibenzo-p-dioxin and 3-methylcholanthrene. It has been established that this induction process is dependent on trans-acting factor(s) that include a complex between the environmental chemical inducer and the aromatic hydrocarbon (Ah) receptor. In this report we show that constitutive P(1)450 mRNA but not P(3)450 mRNA is elevated in 7-day-old mouse embryos and following retinoic acid-induced differentiation of F9 embryonal carcinoma cells. In both instances it is shown that P(1)450 gene activation can occur in the absence of a foreign chemical stimulus. These data suggest that the P(1)450 gene may serve an important developmental function during differentiation.
authors: Laboratory of Developmental Pharmacology, National Institute of Child Health and Human Development, Bethesda, MD 20892.
authors: S
authors: S
authors: Kimura
authors: J C
authors: JC
authors: Donovan
authors: D W
authors: DW
authors: Nebert
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D004622:Embryo, Mammalian; D054278:Embryonal Carcinoma Stem Cells; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D006498:Hepatectomy; D008115:Liver Regeneration; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D014411:Neoplastic Stem Cells; D009693:Nucleic Acid Hybridization; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System