title: [Chromatin on a membrane: accessibility of histone H5 for antibodies in the supernucleosomal structure].
abstract: Histone H5 accessibility for the antibodies in chromatin was studied. Chromatin was immobilised on the nitrocellulose membrane in conditions which provide different levels of its compactization. Antiserum specific to the globular domain of histone H5 was used. It was shown, that for establishing real protection of histone H5 in the supernucleosomal structure it is necessary to use long fibers of chromatin. Their linking to the membrane must occur by a minimum quantity of points. It was established, that histone H5 is 5 times more accessive in the preparations of dispersed chromatin (low ionic strength) then in chromatin with the supernucleosomal organization (physiological ionic strength). We suppose that the small level of accessibility of histone H5 for the antibodies in the compact chromatin can be explained by some disruptions in the supernucleosomal organization. On the contrary, the long equable solenoid of nucleosomes provides complete protection of histone H5. In accordance with the results obtained, the model of ordered packaging of nucleosomes in the solenoid is discussed. In this model the point of entrance and exit of DNA on the nucleosomes, fixed by globular region of histone H5, is localized inside the solenoid.
authors: A M
authors: AM
authors: Erkin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D006657:Histones / Q000276:immunology; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D009707:Nucleosomes
chemical_list: D000906:Antibodies; D006657:Histones; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D009707:Nucleosomes
vernacular_title: Khromatin na membrane: issledovanie dostupnosti gistona H5 dlia antitel v nadnukleosomnoĭ strukture.