Performance evaluation of non-porous versus porous ion-exchange packings in the separation of proteins by high-performance liquid chromatography.
The performance of a non-porous, anion-exchange packing was evaluated and compared with a number of similar porous high-performance liquid chromatography packings. The non-porous columns were found to be equally efficient for proteins spanning a wide range of molecular weights, while the porous columns exhibited decreasing efficiency as the proteins became larger. The porous materials also exhibited size exclusion effects that were not seen with the non-porous materials, which partially accounts for the loss of efficiency with large proteins. When increasingly steep gradients were employed, the loss of resolution was less with the non-porous materials.
Duncan
J K
JK
Bio-Rad Laboratories, Richmond, CA 94801.
Chen
A J
AJ
Siebert
C J
CJ
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007475:Ion Exchange Resins; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*
D007475:Ion Exchange Resins; D011506:Proteins