title: The microphotometric determination of the variability of oxygen saturation of erythrocytes lying within rouleaux.
abstract: It was found that by using nonlinear multicomponent analysis with the spectra of oxygenated and deoxygenated haemoglobin as basic spectra the mean O2 saturation of 2 to 3 red cells lying in a rouleau could be determined with an accuracy of 0.5 - 1.0%. At the same PO2 and PCO2 distinct differences in O2 saturation were found in different red cells; for 50% of the saturation values the differences were in the range +/- 1.5%, for 30% of the values, the differences were in the range of +/- 3% and 15% lay in the range of +/- 4.5%. Only 5% of saturation differences were larger than 4.6%. These differences are so large that they have to be considered in calculations of the PO2 from O2 saturation measurements.
authors: Max-Planck-Institut fur Systemphysiologie, Dortmund, F.R.G.
authors: D W
authors: DW
authors: Lubbers
authors: H
authors: H
authors: Grisar
authors: T E
authors: TE
authors: Gayeski
mesh_terms: D002448:Cell Adhesion; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D010100:Oxygen / Q000097:blood*; D012016:Reference Values; D013053:Spectrophotometry
chemical_list: D006454:Hemoglobins; D010100:Oxygen