Measurement of a glycoprotein with blood group A activity by light scattering.
A light scattering method for quantifying glycoproteins is presented. Conditions of ethanol concentration, wavelength, time of reading, and sample and reagent volumes have been examined. The definitive assay involves addition of 9 vol absolute ethanol to 1 vol sample, mixing, and readings at 105 degrees angle at the interval between 10 and 13 min after placing the cell into the photometer. The obtained values are temperature-dependent. Thus, temperature control is essential. In this assay, a concentration range of 10-60 micrograms glycoprotein has been used. As described, the method is independent of the chemical composition of the different glycoproteins and is barely influenced by size and shape of glycoproteins.
Estelrich
J
J
Departament de Fisicoquímica Biológica, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Pouplana
R
R
D000017:ABO Blood-Group System; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008027:Light; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000379:methods; D013816:Thermodynamics
D000017:ABO Blood-Group System; D006023:Glycoproteins