title: Characterization of N-linked gluco-oligomannose type of carbohydrate chains of glycoproteins from the ovary of the starfish Asterias rubens (L.).
abstract: Glycoproteins were isolated from the ovary of the starfish Asterias rubens (L.). After delipidation, sugar analysis revealed the presence of mannose, glucose and N-acetylglucosamine in a molar ratio of 9.0:1.3:2.3. Subsequently, hydrazinolysis, re-N-acetylation, reduction and high-voltage paper electrophoresis were carried out, resulting in a mixture of neutral oligosaccharide alditols which was fractionated on Bio-Gel P-4. The alditols, investigated by 500-MHz 1H-NMR spectroscopy, turned out to be of the oligomannose type or of the gluco-oligomannose type containing 9 mannose and 1-3 glucose residues. The most abundant compounds were established to be: (Formula: see text) and (Formula: see text).
authors: Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
authors: P
authors: P
authors: De Waard
authors: J P
authors: JP
authors: Kamerling
authors: H
authors: H
authors: Van Halbeek
authors: J F
authors: JF
authors: Vliegenthart
authors: J J
authors: JJ
authors: Broertjes
mesh_terms: D000117:Acetylglucosamine / Q000302:isolation & purification; D000818:Animals; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000302:isolation & purification*; D004590:Electrophoresis, Paper; D004735:Energy Transfer; D005260:Female; D005947:Glucose / Q000302:isolation & purification; D006023:Glycoproteins / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000302:isolation & purification; D010053:Ovary / Q000032:analysis; D013215:Starfish / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D006023:Glycoproteins; D005947:Glucose; D008358:Mannose; D000117:Acetylglucosamine