A modified trans-membrane migration method for measuring the effect of drugs on sperm motility.
1. The trans-membrane migration ratio (TMMR) technique is a simple method for quantitating and comparing the effect of drugs on sperm motility. 2. The ultrafilter membrane (Nucleopore) used allows mixing between the solutions on either side resulting in 21-fold dilution of a drug initially mixed with semen. 3. The concentration of drug originally mixed with semen does not remain constant over the incubation time. 4. The phosphate buffer saline (PBS) in the lower chamber should be replaced by PBS containing the same concentration of drug as in the upper chamber so that a homogenous system is established throughout the incubation. 5. Reports of previous studies using this technique may have underestimated the effect of drugs on sperm motility.
Raoof
N T
NT
Department of Clinical Pharmacology, St Bartholomew's Hospital Medical College, London.
Pearson
R M
RM
Turner
P
P
D001692:Biological Transport; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008567:Membranes, Artificial; D011433:Propranolol / Q000493:pharmacokinetics / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects*
D008567:Membranes, Artificial; D011433:Propranolol