Determination of reducing and non-reducing carbohydrates in food products by liquid chromatography with post-column catalytic hydrolysis and derivatization. Comparison with refractive index detection.
Post-column catalytic hydrolysis combined with 4-aminobenzoic acid hydrazide derivatization is employed for the determination of both reducing and non-reducing carbohydrates in a variety of complex sample matrices such as dairy products, processed foods and tobaccos. Comparison with refractive index detection shows the post-column method to be superior from the standpoint of selectivity, sensitivity and simplicity of sample preparation. Limits of detection are in the low-nanogram range. Quantitative results are presented for the determination of sugars in potato extracts. The method is also applicable for the separation and detection of carbohydrate oligomers.
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ABI Analytical, Kratos Division, Ramsey, NJ 07446.
Weinberger
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D001628:Beverages / Q000032:analysis; D002384:Catalysis; D002853:Chromatography, Liquid; D003611:Dairy Products / Q000032:analysis; D004040:Dietary Carbohydrates / Q000032:analysis*; D002523:Edible Grain / Q000032:analysis; D005504:Food Analysis; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis; D012031:Refractometry; D014675:Vegetables / Q000032:analysis
D004040:Dietary Carbohydrates; D009844:Oligosaccharides