Evaluation of a method of production and purification of monoclonal antibodies for clinical applications.
A procedure is described for the purification of monoclonal antibodies (Mab) from ascitic fluids, which meets the quality control required for in vivo applications of immunoglobulins (Ig) in man. Additional assays were performed to calculate viral and DNA content of the purified Mab. These studies are important to prevent the possible side effects, oncogenic events and virus-related diseases which could follow immunotherapy with Mab.
De Rie
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MA
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Zeijlemaker
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WP
Von dem Borne
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AE
Out
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TA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification* / Q000627:therapeutic use; D001202:Ascitic Fluid / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005590:Fractional Precipitation; D051379:Mice
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D004247:DNA