[Effect of monooxygenase reactions catalyzed by cytochrome P-450 on the microsomal membrane].
Hydroxylation of dimethylaniline in rabbit liver microsomes is accompanied by inactivation of cytochrome P-450 and the formation of products inhibiting the catalytic activity of non-inactivated cytochrome P-450. Other enzymes and electron carriers of microsomal membrane (cytochrome b5, NADH-ferricyanide reductase, NADPH-cytochrome c and NADPH-cytochrome P-450 reductases) as well as glucose-6-phosphatase were not inactivated in the course of the monooxygenase reactions. Phospholipids and microsomal membrane proteins were also unaffected thereby. Consequently, the changes in the microsomal membrane during cytochrome P-450 dependent monooxygenase system functioning are confined to the inactivation of cytochrome P-450.
Karuzina
I I
II
Mengazetdinov
D E
DE
Kapitanov
A B
AB
Zhukov
A A
AA
Ivanova
L I
LI
D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism; D015786:Cytochromes b5; D005557:Formaldehyde / Q000378:metabolism; D007425:Intracellular Membranes / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009249:NADP; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011817:Rabbits; D013379:Substrate Specificity
D000814:Aniline Compounds; D065607:Cytochrome P-450 Enzyme Inhibitors; D003573:Cytochrome b Group; D005557:Formaldehyde; D009249:NADP; C015157:N,N-dimethylaniline; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases
Vliianie monooksigenaznykh reaktsiĭ, kataliziruemykh tsitokhromom P-450, na mikrosomal'nuiu membranu.