title: III6NeuAcLc4Cer in human SW1116 colorectal carcinoma cells: a possible oncofetal antigen that is not dependent on Lewis gene expression.
abstract: Monospecific rabbit antibodies directed against the human milk sialyloligosaccharides III6NeuAcLcOse4 (sialyltetrasaccharide b), IV3NeuAcLcOse4 (sialyltetrasaccharide a), and IV6NeuAcnLc4Ose (sialyltetrasaccharide c) were used to detect their homologous haptens as gangliosides or ganglioside-derived sialyloligosaccharides from the human colorectal carcinoma cell line SW1116. III6NeuAcLc4Cer was first detected in human meconium [P. A. Prieto and D. F. Smith (1985) Arch. Biochem. Biophys. 241, 281-289], and its presence in a total ganglioside fraction of SW1116 cells together with its absence from a total lipid extract of normal human intestinal mucosa are consistent with III6NeuAcLc4Cer being a tumor-associated oncofetal antigen. IV3NeuAcLc4Cer, a ganglioside in human meconium [P. A. Prieto and D. F. Smith (1986) Arch. Biochem. Biophys. 249, 243-253], was also detected in SW1116 cells; an observation that is consistent with its being the immediate precursor to the sialyl-Lea ganglioside in SW1116 cells. Specific antisera against sialylated type 1 oligosaccharide chains whose expression is independent of the Lewis gene fucosyltransferase may be useful diagnostic reagents for oncofetal, carbohydrate antigens.
authors: Department of Biochemistry and Nutrition, Virginia Tech, Blacksburg 24061.
authors: K L
authors: KL
authors: Law
authors: D F
authors: DF
authors: Smith
mesh_terms: D000906:Antibodies; D000951:Antigens, Neoplasm; D002240:Carbohydrate Sequence; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology*; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D009844:Oligosaccharides / Q000302:isolation & purification; D012004:Rectal Neoplasms / Q000276:immunology*
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D006017:Glycolipids; D009844:Oligosaccharides; C031192:oncofetal antigens; C009255:sialoglycolipids; C016735:sialooligosaccharides