title: The identification of serum tuftsin by reverse-phase high-performance liquid chromatography and mass spectrometry.
abstract: We describe for the first time a method for unambiguously identifying the phagocytic stimulating tetrapeptide tuftsin from trypsinized human serum. The method consists of separating serum tuftsin by reverse-phase (RP)-HPLC, collecting HPLC fractions corresponding to the synthetic tuftsin retention time, and then subjecting those fractions to mass spectrometry/mass spectrometry (MS/MS) analysis, which provides optimal molecular specificity to the measurement. Although quantification was not the goal, it was estimated that the amount of tuftsin found by RP-HPLC and MS/MS was in the hundreds of nanograms per milliliter.
authors: Department of Surgery, Rochester General Hospital, New York 14621.
authors: J O
authors: JO
authors: Naim
authors: D M
authors: DM
authors: Desiderio
authors: J
authors: J
authors: Trimble
authors: J R
authors: JR
authors: Hinshaw
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D014405:Tuftsin / Q000097:blood*
chemical_list: D014405:Tuftsin