Acid phosphatases of the mosquito Culex tarsalis coquillett.
1. Spectrophotometric and isoelectric focusing (IEF) electrophoretic characterization of the acid phosphatases (ACP) of the mosquito, Culex tarsalis, are presented. 2. ACP hydrolysis of P-nitrophenylphosphate (Pnp) was optimal at 37 degrees C, pH 5.25 in the presence of 15 mM MgCl2 and 0.1% (w/v) polyvinylpyrollidone (PVP). Vmax and Km values varied significantly between the various mosquito strains examined. 3. Several divalent cations (i.e. Mn2+, Ca2+, Ba2+ and Co2+), either the chloride or sulphate salts, were stimulatory for ACP. Both Cu2+ and Fe2+ (15 mM) were inhibitory. 4. Slight inhibition (i.e. 10%) of ACP activity was observed with dithiothreitol (100 mM) and 50% inhibition by cysteine (100 mM). 5. ACP activity was cyclic during the 15-day post-adult emergence period of the study. No significant differences were noted between the ACP specific activities of males and females nor between geographic strains. 6. IEF electrophoresis revealed three alpha-naphthyl phosphate hydrolytic ACP isozymes within the pH 4.5-5.5 range (i.e. ACP4.8, ACP5.3 and ACP5.5). 7. IEF ACP isozymes were stimulated by PVP, Mg2+, Zn2+ and inhibited by cysteine, EDTA (except ACP5.3) and NaFl. 8. IEF detection of ACP with Pnp revealed an ACP isozyme (ACP4.3) distinct from those ACP isozymes capable of alpha-naphthyl phosphate hydrolysis.
Houk
E J
EJ
School of Public Health, Naval Biosciences Laboratory, University of California, Berkeley 94720.
Hardy
J L
JL
D000135:Acid Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002413:Cations, Divalent; D002467:Cell Nucleus / Q000201:enzymology; D003465:Culex / Q000201:enzymology*; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology
D002413:Cations, Divalent; D000135:Acid Phosphatase