title: Abrupt loss of dependence of retinopetal neurons on their target cells, as shown by intraocular injections of kainate in chick embryos.
abstract: We have examined the capacity of neurons in the chick isthmo-optic nucleus (ION) to survive when their target neurons in the contralateral retinal are destroyed by intraocular injections of kainate (KA) at different stages in development. The retinal vulnerability to KA builds up progressively from embryonic day 10 (E10) until a plateau is reached at E15 (see accompanying paper); and the effects on the ION increase in parallel, almost all the ION neurons being rapidly lost after the E15 injections. KA injection before E15 lesioned only part of the retina and caused degeneration only in the topographically corresponding region of the ION. Near the end of the natural cell death period in the ION (E17), this initial dependence on the target cells is rapidly lost. Already at E16 the injections kill less ION neurons, and by E19 they kill none of them. The ION neurons have become completely insensitive to the KA injections and appear normal more than 4 months later, although axotomy (by eye removal) at a similar age would by then have killed them. The ectopic ION neurons, scattered outside the ION but projecting to the retina, are never affected by KA injections at any age.
authors: Institute of Anatomy, University of Lausanne, Switzerland.
authors: S
authors: S
authors: Catsicas
authors: P G
authors: PG
authors: Clarke
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo / Q000502:physiology*; D003078:Colchicine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005123:Eye; D007267:Injections; D007608:Kainic Acid / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009900:Optic Nerve / Q000166:cytology / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D007608:Kainic Acid; D003078:Colchicine