Bovine serum hemopexin: properties of the protein from a single animal.
1. Hemopexin was isolated from bovine serum of a single animal in a yield of 0.5 mg/ml. 2. Bovine hemopexin was found to exist in two isoforms of mol. wt 68,000 and 65,000. 3. Treatment of hemopexin with glycopeptidase F yields a single band corresponding to a mol. wt of 51,000. 4. The protein binds heme on an equimolar ratio and shows a single component in reverse-phase high performance liquid chromatography. 5. The amino acid composition of bovine hemopexin compares with that of hemopexin isolated form other animals.
Noiva
R
R
Department of Biological Chemistry, Creighton University School of Medicine, Omaha, NE 68178.
Pete
M J
MJ
Babin
D R
DR
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle / Q000097:blood*; D006418:Heme / Q000378:metabolism; D006466:Hemopexin / Q000302:isolation & purification*; D008297:Male; D008970:Molecular Weight; D009919:Orchiectomy; D011485:Protein Binding; D051381:Rats; D013045:Species Specificity
D000596:Amino Acids; D006418:Heme; D006466:Hemopexin