Hairy cell leukemia: in-vitro proliferation and pseudo-colony formation.
In an established double layer clonogenic assay, the PHA-leukocyte feeder colony assay, hairy cell leukemia (HCL) cells formed strong aggregates simulating colonies. After irradiation with 50 Gy, colony formation persisted. Even in a modified colony assay consisting of agar 0.5% overlayered by methylcellulose 0.9%, cell aggregation was still possible due to increasing fluidity of the methylcellulose during the culture period. Time-lapse video recordings confirmed prominent cell motility leading to pseudo-colony formation. Studies with bromodeoxyuridine incorporation showed a low proliferation index (up to 13%) of hairy cells. In conclusion, any assay that facilitates cell motility is unsuitable to study HCL colony growth.
Kluin-Nelemans
J C
JC
Dept. of Hematology, University Hospital, Leiden, The Netherlands.
Hakvoort
H W
HW
van Dissel
J T
JT
van Dierendonck
J H
JH
Beverstock
G C
GC
Fibbe
W E
WE
Willemze
R
R
D001973:Bromodeoxyuridine / Q000378:metabolism; D002449:Cell Aggregation; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*
D001973:Bromodeoxyuridine