[Study of the interaction of low-density lipoproteins with immobilized fibrinogen and fibronectin using an immunoenzyme assay].
The interaction of serum low density lipoproteins (LDL) with fibrinogen, fibronectin and fibrinogen-fibronectin complex immobilized on polystyrene was studied. LDL binding by these proteins was assessed by enzyme-linked immunosorbent assay. It was shown that LDL interacts with both immobilized fibronectin and immobilized fibrinogen. The fibrinogen-fibronectin complex bound a greater amount of LDL than either component alone, in the same concentration as in the complex. The binding of LDL with immobilized fibronectin or fibrinogen increased in the presence of low concentrations of diluted fibrinogen or fibronectin, respectively, which suggests the formation of the three-component complex on the surface. The formation of fibrinogen-fibronectin-LDL complexes during homeostasis may promote the accumulation of LDL in the vascular wall.
Chulkova
T M
TM
Panasiuk
A F
AF
D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen; D005353:Fibronectins; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D011485:Protein Binding
D005353:Fibronectins; D008077:Lipoproteins, LDL; D005340:Fibrinogen
Izuchenie vzaimodeĭstviia lipoproteidov nizkoĭ plotnosti s immobilizovannymi fibrinogenom i fibronektinom metodom immunofermentnogo analiza.