[Kinetics of 15 alpha-methyl-8-aza-16-oxagona-1,3,5(10), 13-tetraen-17-ona oxidation by cytochrome P-450 from rat liver microsomes].
The kinetics of oxidation of 15 alpha-methyl-8-aza-16-oxagona-1,3,5(10),13-tetraen-17-on with rat liver microsomal cytochrome P-450 was investigated. The kinetic parameters, Km and Vmax, of the oxidation reaction were found to be equal to 1,3 X 10(-4) M and 4.0 X 10(-7) M X s-1, respectively. Using thin-layer chromatography, mass-spectrometry, PMR-spectroscopy and reciprocal synthesis, it was shown that 3-hydroxy-15 alpha-methyl-8-aza-16-oxagona-1,3,5(10), 13-tetraen-17-on is the main reaction product.
Akhrem
A A
AA
Slepneva
L M
LM
Popova
E M
EM
Lis
L G
LG
Lakhvich
F A
FA
D000818:Animals; D001378:Azasteroids / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006067:Gonanes / Q000378:metabolism*; D006900:Hydroxylation; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D011048:Polarography; D051381:Rats; D013260:Steroids, Heterocyclic / Q000378:metabolism*; D013379:Substrate Specificity
D001378:Azasteroids; D006067:Gonanes; D013260:Steroids, Heterocyclic; D003577:Cytochrome P-450 Enzyme System; C053276:15-methyl-8-aza-16-oxagona-1,3,5(10),13-tetraen-17-one
Kinetika okisleniia 15 alpha-metil-8-aza-16-oksagona-1,3,5(10), 13-tetraen-17-ona tsitokhromom P-450 mikrosom pecheni krys.