[Effect of K+ concentration on the rate of synthesis of fast-labeling proteins in the adrenal cortex].
The effect of K+ concentration on protein biosynthesis and 32P-incorporation into the acid-insoluble fraction of dog and guinea pig adrenal cortex slices was studied. An increase in K+ concentration in the incubation medium from 3 to 8-11 mM induced after 15-20 min of incubation a significant stimulation of 14C-leucine incorporation into the acid-insoluble fraction of post-mitochondrial supernatant. More extensive labelling of this fraction with 32P was observed. Addition of valinomycin caused a shift in the maximum of 14C-leucine incorporation towards lower K+ concentrations. The Na+,K+-ATPase inhibitors--ouabain and strophantin K--reduced the K+-stimulated protein synthesis. These data suggest that K+ transport into the cell is necessary for the stimulating effect to be manifested. Chelation of Ca2+ strongly decreased the incorporation of 14C-leucine into proteins in the presence of 5 mM K+. However, protein labelling increased with a gradual rise in K+ concentration up to 25 mM.
Pushkarev
V M
VM
Tron'ko
N D
ND
Mikosha
A S
AS
D000302:Adrenal Cortex / Q000378:metabolism*; D000450:Aldosterone / Q000096:biosynthesis; D000818:Animals; D004285:Dogs; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008297:Male; D011188:Potassium / Q000494:pharmacology*; D014176:Protein Biosynthesis; D013268:Stimulation, Chemical; D014634:Valinomycin / Q000494:pharmacology
D014634:Valinomycin; D000450:Aldosterone; D007930:Leucine; D011188:Potassium
Vliianie kontsentratsii K+ na skorost' sinteza bystrometiashchikhsia belkov v kore nadpochechnikov.