Prevention of an increase in susceptibility to calcium in a neuroblastoma cell line.
An increase in susceptibility of neuroblastoma cells to calcium was inhibited when the cells were treated with hypothermia, diphenylhydantoin, chlorpromazine or pentobarbital; all of these treatments affect membrane permeability to calcium or membrane-associated cell metabolism. Diphenylhydantoin and chlorpromazine were found to have positive effects only when they were administered before the addition of calcium. This was in contrast to flunarizine, which showed a protective effect only in combination with calcium. However, leupeptin did not show any positive effect, indicating that calcium-activated neutral protease may be only slightly involved in this type of calcium-induced cell damage.
Abe
K
K
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Kogure
K
K
D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology*; D003080:Cold Temperature; D005444:Flunarizine / Q000494:pharmacology*; D006801:Humans; D009447:Neuroblastoma; D010424:Pentobarbital / Q000494:pharmacology; D010672:Phenytoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism
D010672:Phenytoin; D010424:Pentobarbital; D005444:Flunarizine; D002118:Calcium; D002746:Chlorpromazine