Immunocytochemical approach of GABAergic innervation of the mouse spinal cord using antibodies to GABA.
The distribution of gamma-amino-butyric acid containing neurons in the Mouse spinal cord has been studied at both the light and electron microscope levels using antibodies against GABA and revelation by the Fab-peroxidase technique. At the light microscope level immunoreactive profiles of perikarya and neuronal processes were particularly abundant in the superficial laminae (I-IV) of the dorsal horn. Scattered soma profiles were found in the other layers and more particularly in the lamina X where Liquor contacting immuno-reactive neurons could be detected. GABAergic cell bodies were very sparse in the ventral horn. Electron microscopic observations confirmed the light microscope results: terminals constituted synaptic symmetrical contacts that provide a morphological basis for inhibition in the dorsal horn and for post-synaptic inhibition of motoneurons in the ventral horn.
Kaduri
A J
AJ
Laboratoire de Physiologie des Interactions Cellulaires, UA 339 CNRS, Université de Bordeaux I, Talence, France.
Magoul
R
R
Lescaudron
L
L
Campistron
G
G
Calas
A
A
D000818:Animals; D000906:Antibodies; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D013116:Spinal Cord / Q000502:physiology* / Q000648:ultrastructure; D005680:gamma-Aminobutyric Acid / Q000276:immunology / Q000502:physiology*
D000906:Antibodies; D005680:gamma-Aminobutyric Acid