Alkali-labile oligosaccharide units of a sialoglycoprotein from rabbit erythrocyte membranes.
Two glycoproteins (apparent molecular weights 120,000 and 70,000) were extracted from rabbit erythrocyte membranes, and only one (Mr 120,000), which is a sialoglycoprotein, contained O-glycosidically linked sugar chains. Alkali-labile oligosaccharide units of the sialoglycoprotein were released as reduced oligosaccharides by NaOH-NaB3H4 treatment, and then purified by gel filtration on a Bio-Gel P-4 column followed by ion-exchange chromatography. From the results of methylation analysis, mass spectrometry and chromium trioxide oxidation, the main oligosaccharide unit was determined to be a linear trisaccharide (85% by weight), NeuNGc alpha(2----3)Gal beta(1----3)GalNAcol. In addition, small amounts of a tetrasaccharide (11% by weight) and a disaccharide (4% by weight) were found, which were determined to have the following structures, NeuNGc alpha(2----3)Gal beta(1----3)[NeuNGc alpha(2----6)] GalNAcol and Gal-GalNAcol, respectively.
Fukuda
K
K
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Honma
K
K
Manabe
H
H
Utsumi
H
H
Hamada
A
A
D000818:Animals; D002236:Carbohydrate Conformation; D004910:Erythrocyte Membrane / Q000032:analysis*; D006021:Glycophorins / Q000032:analysis*; D013058:Mass Spectrometry; D008745:Methylation; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification*; D011817:Rabbits; D012795:Sialoglycoproteins / Q000032:analysis*
D006021:Glycophorins; D009844:Oligosaccharides; D012795:Sialoglycoproteins