Mannose inhibition as a significant marker for differentiating among novobiocin-resistant staphylococci of relevance in clinical microbiology.
A specific method for the identification of Staphylococcus saprophyticus among novobiocin-resistant species isolated from man is described. The test is based on novobiocin resistance, non-fermentation of D-mannose and early inhibition with late secondary growth on glucose/mannose + novobiocin agar plates. On this medium novobiocin-resistant Staphylococcus cohnii showed a confluent, continuous and homogeneous growth after 24 h which remained unchanged at 48 h whether or not it fermented D-mannose, whereas novobiocin-resistant Staphylococcus xylosus fermented D-mannose. These results are discussed in relation to a possible causal role of PTS enzymes and phosphomannose isomerase deficiency in mannose inhibition.
Cristino
J A
JA
Instituto Bacteriológico Câmara Pestana, University of Lisbon, Portugal.
Pereira
A T
AT
Mira
M L
ML
Manso
C
C
D004352:Drug Resistance, Microbial; D008358:Mannose / Q000037:antagonists & inhibitors*; D009675:Novobiocin / Q000494:pharmacology*; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects*
D009675:Novobiocin; D008358:Mannose