title: Genetic analysis of X-chromosome dosage compensation in Caenorhabditis elegans.
abstract: We have shown that the phenotypes resulting from hypomorphic mutations (causing reduction but not complete loss of function) in two X-linked genes can be used as a genetic assay for X-chromosome dosage compensation in Caenorhabditis elegans between males (XO) and hermaphrodites (XX). In addition we show that recessive mutations in two autosomal genes, dpy-21 V and dpy-26 IV, suppress the phenotypes resulting from the X-linked hypomorphic mutations, but not the phenotypes resulting from comparable autosomal hypomorphic mutations. This result strongly suggests that the dpy-21 and dpy-26 mutations cause increased X expression, implying that the normal function of these genes may be to lower the expression of X-linked genes. Recessive mutations in two other dpy genes, dpy-22 X and dpy-23 X, increase the severity of phenotypes resulting from some X-linked hypomorphic mutations, although dpy-23 may affect the phenotypes resulting from the autosomal hypomorphs as well. The mutations in all four of the dpy genes show their effects in both XO and XX animals, although to different degrees. Mutations in 18 other dpy genes do not show these effects.
authors: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
authors: P M
authors: PM
authors: Meneely
authors: W B
authors: WB
authors: Wood
mesh_terms: D000483:Alleles; D000818:Animals; D002107:Caenorhabditis / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping; D012734:Disorders of Sex Development; D008297:Male; D009154:Mutation; D010641:Phenotype; D014960:X Chromosome