title: Interaction of melittin with negatively charged phospholipids: consequences for lipid organization.
abstract: A characterization of the structural alterations induced by melittin in model-membranes of dioleoylphosphatidic acid and egg phosphatidylglycerol is presented, based on the use of 31P-NMR, freeze-fracture electron microscopy and small angle X-ray scattering. In accordance with earlier findings on the cardiolipin-melittin system, melittin is found to have an inverted phase inducing effect on these negatively charged lipids, in contrast to the influence on zwitterionic phospholipids. In phosphatidic acid this is expressed in the formation of an HII phase; in phosphatidylglycerol a less ordered, non-lamellar structure with low water content is adopted.
authors: Department of Biochemistry, State University of Utrecht, The Netherlands.
authors: A M
authors: AM
authors: Batenburg
authors: J H
authors: JH
authors: van Esch
authors: J
authors: J
authors: Leunissen-Bijvelt
authors: A J
authors: AJ
authors: Verkleij
authors: B
authors: B
authors: de Kruijff
mesh_terms: D001514:Bee Venoms; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005614:Freeze Fracturing; D009682:Magnetic Resonance Spectroscopy; D008555:Melitten; D008563:Membrane Lipids; D008954:Models, Biological; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines; D010743:Phospholipids; D012542:Scattering, Radiation; D014965:X-Rays
chemical_list: D001514:Bee Venoms; D008563:Membrane Lipids; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines; D010743:Phospholipids; D008555:Melitten