Study of human sperm chromosomes by sequential transmission and scanning electron microscopy.
We describe a method of observing human sperm metaphases by sequential transmission and scanning electron microscopy. This permits the analysis of ultrastructural aspects of sperm chromosomes and allows the relationship between ultrastructure, heterochromatin condensation, and the behaviour and staining properties of sperm chromosomes and heterochromatic regions to be determined.
Navarro
J
J
Departament de Biologia Cellular i Fisiologia, Subunitat de Biologia de la Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Benet
J
J
Genescà
A
A
Castell
N
N
Egozcue
J
J
Templado
C
C
D002503:Centromere / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure*; D002878:Chromosomes, Human, Pair 1 / Q000648:ultrastructure; D002885:Chromosomes, Human, Pair 16 / Q000648:ultrastructure; D002899:Chromosomes, Human, Pair 9 / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013094:Spermatozoa / Q000648:ultrastructure*; D014960:X Chromosome / Q000648:ultrastructure; D014998:Y Chromosome / Q000648:ultrastructure
D002843:Chromatin