Idiograms of horse chromosomes at prometaphase, early metaphase, and midmetaphase after R-banding by BrdU incorporation followed by the fluorochrome-photolysis-Giemsa technique.
We present three idiograms of equine chromosomes, R-banded after BrdU incorporation and stained by the fluorochrome-photolysis-Giemsa technique. The haploid set of prometaphasic chromosomes shows 591 bands (range 7-38 per chromosome), the early metaphasic set 404 (range 5-26), and the midmetaphasic set 272 (range 3-18). Following cell synchronization with thymidine, more than twice as many R-bands were revealed on the resulting prometaphasic chromosomes, making possible the establishment of a very accurate and characteristic representation of this banding pattern in the domestic horse.
Romagnano
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Département d'anatomie, Faculté de médecine, Université de Montréal, Qué., Canada.
Drouin
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R
Richer
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CL
D000818:Animals; D001399:Azure Stains; D001973:Bromodeoxyuridine; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D006736:Horses / Q000235:genetics*; D008677:Metaphase; D010782:Photolysis
D001399:Azure Stains; D005456:Fluorescent Dyes; D001973:Bromodeoxyuridine