Circadian modification of 5-fluorouracil-induced teratogenesis in mice.
Pregnant mice were treated intraperitoneally with 5-fluorouracil in a single dose of 30 mg/kg once on gestation day 11 at one of four selected times along the 24-hr time scale. All animals were kept under a lighting regimen of 12 hr (0600-1800) alternating with 12 hr of darkness. They were injected at the same circadian phase as they were mated. The developmental age of all fetuses was 264 +/- 1 hr at the time of injection. Fetuses collected on day 18 showed a circadian variation in teratogenic susceptibility to 5-fluorouracil. The lethal and teratogenic risk posed by the drug were highest among animals treated at the mid-light period until the onset of darkness.
Sauerbier
I
I
Department of Anatomy, Medical School Hannover, FRG.
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000818:Animals; D002940:Circadian Rhythm; D005260:Female; D005472:Fluorouracil / Q000633:toxicity*; D005865:Gestational Age; D011247:Pregnancy; D051381:Rats; D013723:Teratogens
D013723:Teratogens; D005472:Fluorouracil