Chemical structure of three neutral trisaccharides isolated in free form from bovine colostrum.
Three neutral trisaccharides, which comprise 25.1% of the neutral oligosaccharide other than lactose, were isolated from bovine colostrum, obtained 6 h after parturition, by l.c. on amino silica gel. The chemical structures were identified, by methylation analysis with direct m.s. and g.l.c.-m.s., and by structural analysis with 13C-n.m.r., as beta-D-Galp-(1----4)-[alpha-L-Fucp-(1----3)-]-D-GlcNAc (3-fucosyl-N-acetyllactosamine), beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D-Glc (3'-galactosyllactose), and beta-D-Galp-(1----6)-beta-D-Galp-(1----4)-D-Glc (6'-galactosyllactose). The The first-named compound was a novel oligosaccharide from mammalian milk.
Saito
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Laboratory of Food Hygiene, Faculty of Social Welfare, Tohoku Fukushi University, Sendai, Japan.
Itoh
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Adachi
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D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D003126:Colostrum / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000302:isolation & purification*; D011247:Pregnancy
D009844:Oligosaccharides