Eukaryotic elongation factor Tu is present in mRNA-protein complexes.
By two-dimensional gel electrophoresis, partial peptide mapping, and antibody binding we have shown that eukaryotic elongation factor Tu is in close contact with mRNA in rabbit reticulocytes. It can be crosslinked to mRNA by irradiating both polysomes and 40-80 S mRNA-protein complexes with short-wave UV light. To our knowledge this is the first case in which a known translation factor has been shown to be associated with mRNA in native ribonucleoproteins.
Greenberg
J R
JR
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
Slobin
L I
LI
D000818:Animals; D010444:Peptide Elongation Factor Tu / Q000032:analysis* / Q000528:radiation effects; D010449:Peptide Mapping; D012333:RNA, Messenger / Q000032:analysis / Q000528:radiation effects; D011817:Rabbits; D012261:Ribonucleoproteins / Q000032:analysis* / Q000528:radiation effects; D014466:Ultraviolet Rays
D012333:RNA, Messenger; D012261:Ribonucleoproteins; D010444:Peptide Elongation Factor Tu