Investigation of chromosome-mediated gene transfer using the HPRT region of the human X chromosome as a model.
A panel of over 50 hybrid cells containing varying portions of the long arm of the human X chromosome have been obtained by chromosome-mediated gene transfer (CMGT) of human chromosomes to mouse cells deficient in HPRT. This panel is used to investigate the size and integrity of transfected human chromosome fragments and also to examine the effect of including a selectable DNA plasmid in the transfection mix. Chromosomal rearrangements are found to be generated in the chromosome transfer process, and the human X centromeric region is detected in the transfected cells at an unusually high frequency. Extensive lengths of X chromosome DNA are transferred intact, suggesting potential uses of CMGT in cloning large genes and loci for which only the chromosomal map position is known.
Pritchard
C A
CA
Laboratory of Human Molecular Genetics, Imperial Cancer Research Fund, London, UK.
Goodfellow
P N
PN
D000818:Animals; D002503:Centromere / Q000648:ultrastructure; D002874:Chromosome Mapping; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D051379:Mice; D014162:Transfection; D014960:X Chromosome
D007527:Isoenzymes; D007041:Hypoxanthine Phosphoribosyltransferase