title: Structural and functional characterization of a Dictyostelium gene encoding a DIF inducible, prestalk-enriched mRNA sequence.
abstract: The pDd56 mRNA sequence is highly enriched in prestalk over prespore cells and is inducible by DIF, the putative Dictyostelium stalk-specific morphogen. We show that the pDd56 gene is composed of forty one copies of a twenty four amino acid, cysteine rich repeat. This is highly homologus to a repeat which we have previously shown to compose the major fraction of the pDd63 mRNA, another DIF inducible, prestalk-enriched sequence. The predicted pDd56 protein contains a putative signal peptide but does not appear to contain a transmembrane segment. In combination these features suggest it to be an extrinsic protein and we confirm this elsewhere by showing that the pDd56 gene encodes a known, extracellular protein of the stalk. The pDd56 mRNA is dependent upon exogenous DIF for its accumulation. We show that this control is exerted at the transcriptional level and that a restriction fragment containing 1.7Kb of upstream sequence directs temporally-regulated expression of the gene.
authors: Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire, UK.
authors: A
authors: A
authors: Ceccarelli
authors: S J
authors: SJ
authors: McRobbie
authors: K A
authors: KA
authors: Jermyn
authors: K
authors: K
authors: Duffy
authors: A
authors: A
authors: Early
authors: J G
authors: JG
authors: Williams
mesh_terms: D000595:Amino Acid Sequence; D004274:DNA, Recombinant; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development; D005656:Fungal Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005800:Genes, Fungal; D006588:Hexanones / Q000494:pharmacology*; D007659:Ketones / Q000494:pharmacology*; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004274:DNA, Recombinant; D005656:Fungal Proteins; D006588:Hexanones; D007659:Ketones; D012331:RNA, Fungal; D012333:RNA, Messenger; C053433:1-((3,5-dichloro)-2,6-dihydroxy-4-methoxyphenyl)-1-hexanone