High-performance liquid chromatographic analysis of ascorbate-2-phosphate, adenine and hypoxanthine in stored human blood.
A high-performance liquid chromatographic procedure was developed to assay for adenine, hypoxanthine, and ascorbate-2-phosphate in both red blood cells and plasma. The samples were diluted and filtered through Centriflo filter cones to remove most of the blood proteins before injection onto the column. The application of the technique to an experimental blood preservation study is illustrated.
Moore
G L
GL
Letterman Army Institute of Research, Presidio of San Francisco, CA 94129.
Fishman
R M
RM
D000225:Adenine / Q000097:blood*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000032:analysis; D006801:Humans; D007042:Hypoxanthines / Q000097:blood*; D010949:Plasma / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D007042:Hypoxanthines; C011669:ascorbate-2-phosphate; D000225:Adenine; D001205:Ascorbic Acid