Calcium influx in a single rat basophilic leukemia cell as revealed with a digital imaging fluorescence microscope.
Using a digital imaging fluorescence microscope, we have detected a rapid transient increase in the free cytosolic calcium concentration in a single rat basophilic leukemia cell (RBL-2H3) after antigen stimulation. Calcium ions were transported very rapidly (within 1 s) after a lag time (about 10 s at 37 degrees C) from the external environment into the cytoplasm. On the basis of the present experimental results we conclude that the gradual changes in the overall fluorescence intensity observed for a cell suspension are due to the distribution of different lag times shown by different cells as to the calcium influx through membrane calcium channels.
Kato
K
K
Faculty of Pharmaceutical Sciences, University of Tokyo.
Nakanishi
M
M
Arata
Y
Y
Teshima
R
R
Terao
T
T
Miyamoto
H
H
D000818:Animals; D001491:Basophils / Q000378:metabolism; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D016257:Fura-2; D007942:Leukemia, Experimental / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D051381:Rats
D001572:Benzofurans; D005456:Fluorescent Dyes; D002118:Calcium; D016257:Fura-2