title: Efficient encapsulation of water-soluble materials into small-sized liposomes.
abstract: A large proportion of the aqueous phase was encapsulated into small-sized liposomes with diameters less than 100 nm, when multilamellar liposomes, prepared by vortexing a lipid film at an extremely high concentration of lipids up to 512 mM, were subsequently rendered to small vesicles by sonication. Sixty percent of adriamycin (8.6 mM) and 39% of sucrose (50 mM) presented were respectively entrapped into the vesicles prepared with 256 mM lipids. The vesicles formed were quite stable and retained a major portion of the entrapped materials. This simple procedure may be useful for encapsulation of valuable, water-soluble materials into small-sized liposomes.
authors: Laboratory of Biological Products, University of Tokyo, Japan.
authors: T
authors: T
authors: Tomita
authors: T
authors: T
authors: Tadakuma
authors: K
authors: K
authors: Tomochika
authors: T
authors: T
authors: Yasuda
mesh_terms: D004317:Doxorubicin; D004355:Drug Stability; D008081:Liposomes; D012995:Solubility; D013395:Sucrose; D014867:Water
chemical_list: D008081:Liposomes; D014867:Water; D013395:Sucrose; D004317:Doxorubicin