Identification of two variants of troponin T in the developing chicken heart using a monoclonal antibody.
Troponin T (TNT) expressed in the developing chicken cardiac muscle was examined by immunoblotting combined with two-dimensional electrophoresis (2-D PAGE) and peptide mapping. When the whole lysate of the neonatal heart was examined by 2-D PAGE, two TNT variants were detected on the gel by monoclonal antibody to TNT. Expression of the two variants was developmentally regulated: one isoform (type I) was expressed from embryonic through neonatal stages, and the other (type II) from the late embryonic stage through adulthood during cardiac muscle development. The type-I isoform, but not type-II isoform, was also expressed transiently in chicken skeletal muscle at embryonic stages. As judged from the peptide maps, the two isoforms differed in the N-terminal region but not in the C-terminal region.
Ogasawara
Y
Y
Department of Biology, Faculty of Science, Chiba University.
Komiya
T
T
Obinata
T
T
D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006321:Heart / Q000196:embryology / Q000254:growth & development*; D010449:Peptide Mapping; D014336:Troponin / Q000032:analysis / Q000235:genetics*; D020107:Troponin T
D000911:Antibodies, Monoclonal; D014336:Troponin; D020107:Troponin T