Application of semi-preparative high-performance liquid chromatography to difficult natural product separations.
Semi-preparative high-performance liquid chromatography on RP-18 with methanol-water as the mobile phase was used for the separation of homologous antifungal chromenes and related homologous dichromenes from Hypericum revolutum Vahl (Guttiferae). In the course of the structural elucidation of new saponins from Phylolacca dodecandra L'Hérit. (Phytolaccaceae), two isomeric oleanane saponins were separated on RP-8 with acetonitrile-water as the mobile phase.
Décosterd
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LA
Institut de Pharmacognosie et Phytochimie, Ecole de Pharmacie de l'Université de Lausanne, Switzerland.
Dorsaz
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AC
Hostettmann
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K
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D007536:Isomerism; D010946:Plants, Medicinal / Q000032:analysis*; D012503:Saponins / Q000302:isolation & purification
D012503:Saponins