Blood pressure and metabolic effects of 18-oxo-cortisol in sheep.
18-Oxo-cortisol (18-oxo-F) has been isolated from the urine of subjects with primary aldosteronism. This study examines the pressor, mineralocorticoid and glucocorticoid effects of 18-oxo-F in conscious sheep--a well studied species for the assessment of the pressor effect of steroid hormones. 18-oxo-F (24 mg/day i.v. for 5 days, n = 3) increased mean arterial pressure MAP (64 +/- 2 mmHg control and 75 +/- 6 mmHg on day 5 P less than 0.001). There was no change in heart rate. Plasma [K+] decreased from a control of 4.3 +/- 0.1 mmol/l control to 2.9 +/- 0.3 mmol/l on day 5 (P less than 0.001). Urinary Na+ excretion decreased on the first infusion day (233 +/- 18 mmol/day control and 124 +/- 20 mmol/day on infusion day 1 P less than 0.001). Urinary K+ excretion was reduced on days 1, 4 and 5 of the infusion. Thus in sheep, 18-oxo-F increased blood pressure associated with in vivo evidence of mineralocorticoid activity.
Spence
C D
CD
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Australia.
Coghlan
J P
JP
Denton
D A
DA
Gomez-Sanchez
C
C
Mills
E H
EH
Whitworth
J A
JA
Scoggins
B A
BA
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D004231:Diuresis / Q000187:drug effects; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000494:pharmacology; D008901:Mineralocorticoids / Q000502:physiology; D011188:Potassium / Q000097:blood / Q000652:urine; D012756:Sheep; D012964:Sodium / Q000097:blood / Q000652:urine
D008901:Mineralocorticoids; C038135:18-oxocortisol; D012964:Sodium; D011188:Potassium; D006854:Hydrocortisone