Use of biotinylated probes in serum hepatitis B virus DNA detection.
A dot-blot hybridisation assay for serum HBV DNA is described using a non-radioactive (biotinylated) DNA probe. The assay is both sensitive (1 pg of HBV DNA) and reproducible, and shows several advantages over similar assays which use 32P-labelled probes for the routine detection of infectious virus particles both in blood and in other biological fluids.
Saldanha
J A
JA
Department of Physiology, Royal Free Hospital School of Medicine, London, U.K.
Karayiannis
P
P
Thomas
H C
HC
Monjardino
J P
JP
D001710:Biotin; D004279:DNA, Viral / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011933:Reagent Kits, Diagnostic
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic; D001710:Biotin