title: Involvement of tyrosine protein kinase in the initiation of flagellar movement in rainbow trout spermatozoa.
abstract: Initiation of flagellar motility in spermatozoa of the rainbow trout, Salmo gairdneri, is closely related to phosphorylation of a protein of molecular mass 15 kDa (Morisawa, M., and Hayashi, H. (1986) Biomed. Res. 6, 181-184). We have been able to solubilize the protein and its kinase and then construct an assay system in vitro for the phosphorylation of the 15-kDa protein. In vitro, the protein was phosphorylated in a cAMP-dependent manner. The phosphorylation absolutely required the presence of Mg2+ ions. at millimolar concentrations, but not of Ca2+ ions. The amino acid residue which was phosphorylated in the 15-kDa protein was tyrosine. The 15-kDa protein was purified to near homogeneity by affinity chromatography on a column of adenosine nucleotides conjugated to Eupergit and ion-exchange chromatography on DEAE-cellulose. The effects of synthetic inhibitors of protein kinase on the phosphorylation of the 15-kDa protein were also studied.
authors: Institute of Molecular Biology, Faculty of Science, Nagoya University, Japan.
authors: H
authors: H
authors: Hayashi
authors: K
authors: K
authors: Yamamoto
authors: H
authors: H
authors: Yonekawa
authors: M
authors: M
authors: Morisawa
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002474:Cell-Free System; D005407:Flagella / Q000502:physiology*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D012016:Reference Values; D012487:Salmonidae / Q000502:physiology*; D013081:Sperm Motility; D013997:Time Factors; D014337:Trout / Q000502:physiology*
chemical_list: D011505:Protein-Tyrosine Kinases; D008274:Magnesium; D002118:Calcium