An improved method for elimination of mycoplasmas from cell cultures.
Cell lines infected by different species of mycoplasma (Mycoplasma orale, Mycoplasma hominis) were decontaminated by co-culture with human blood monocyte (BM)-derived macrophages and pooled human immunoglobulin preparations. Co-cultures with BM-derived macrophages or murine peritoneal macrophages (PM) alone were not successful. The phenotype of infected cell lines did not differ from that of uninfected cell lines as revealed by morphological, enzymecytochemical, and immunocytochemical analysis.
Kreipe
H
H
Institute of Pathology, University of Kiel, F.R.G.
Radzun
H J
HJ
Keulers
A
A
Parwaresch
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology*; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000276:immunology; D009174:Mycoplasma / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins