title: Bile acids inhibit secretion of very low density lipoprotein by rat hepatocytes.
abstract: The influence of taurocholate on very low density lipoprotein (VLDL) triacylglycerol synthesis and secretion was studied by isolated rat liver-parenchymal cells. The incorporation of [3H]glycerol into cell-associated and VLDL triacylglycerols were measured after incubation in medium containing 0.75 mM oleate. Taurocholate caused a maked decrease in VLDL [3H]triacylglycerol secretion from the hepatocytes: 50-150 microM taurocholate inhibited secretion of VLDL [3H]triacylglycerols by 70-90%. Similar results were obtained when the mass of secreted VLDL triacylglycerols was measured. Taurocholate caused a decreased secretion of VLDL [3H]triacylglycerols after 15-30 min incubation. A higher amount of cellular triacylglycerols was found in taurocholate-supplemented cells. Furthermore taurocholate did not change the intracellular lipolysis of triacylglycerols. These results suggest that bile acids interfere more probably with the assembly and/or secretion of VLDL-particles and not with earlier stages of VLDL formation, e.g. triacylglycerol synthesis.
authors: Institut für Physiologie und Biochemie der Ernährung der Bundesanstalt für Milchforschung, Kiel.
authors: R
authors: R
authors: del Pozo
authors: C A
authors: CA
authors: Barth
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism; D066298:In Vitro Techniques; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001647:Bile Acids and Salts; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D005990:Glycerol