title: Impaired neutrophil function during anesthesia and surgery is due to serum factors.
abstract: Neutrophil function was assessed in patients undergoing anesthesia and surgery using a chemiluminescence (CL) assay. With the anesthetic agents enflurane and nitrous oxide, peroperative CL (99.1 mV; 13.8 SEM: postinduction but prior to surgery) was significantly lower than the preoperative value (146.5 mV; 14.1 SEM) with a mean fall of 30% (P less than 0.001). CL measurements taken 24 hr postoperatively were significantly increased (193.9 mV; 16.4 SEM) over the pre- and peroperative values, showing mean increases of 32 and 96%, respectively (P less than 0.001 in both cases). The inhibitory influence on CL appeared to be due to serum factors since peroperative patients' sera inhibited control neutrophils. Significantly depressed levels of the complement component C3 and IgG detected during the peroperative period (P less than 0.05) may explain this phenomenon. Postoperatively, C3 and IgG levels returned to normal. The transient decrease in peroperative neutrophil function may be a contributory factor to the establishment of postoperative sepsis in surgical patients.
authors: Department of Surgery, Trinity College, Dublin, Ireland.
authors: K
authors: K
authors: Mealy
authors: C
authors: C
authors: O'Farrelly
authors: R
authors: R
authors: Stephens
authors: C
authors: C
authors: Feighery
mesh_terms: D000769:Anesthesia, Inhalation; D003176:Complement C3 / Q000032:analysis; D004737:Enflurane; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D013514:Surgical Procedures, Operative; D013530:Surgical Wound Infection / Q000276:immunology
chemical_list: D003176:Complement C3; D007074:Immunoglobulin G; D004737:Enflurane