The detection of heat-aggregated IgG (as a model for immune complexes) by reverse passive haemagglutination using a human monoclonal rheumatoid factor coupled to erythrocytes.
A human monoclonal IgM rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus (EBV)-immortalized cell line was purified by protein A-Sepharose adsorption and coupled by the chromic chloride method to human erythrocytes. The RF-coupled cells were incorporated in reverse passive haemagglutination (RPH) assays to detect immune complexes (IC) using heat-aggregated human IgG as a model system. The sensitivity of the RPH was comparable to an enzyme-linked immunosorbent assay (ELISA) using sheep C1q for the detection of ICs.
Welsh
C J
CJ
University of Cambridge, Department of Pathology, U.K.
Steinitz
M
M
Clark
M R
MR
Coombs
R R
RR
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis*; D004912:Erythrocytes; D006386:Hemagglutination Tests; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D011108:Polymers; D012217:Rheumatoid Factor
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D011108:Polymers; D012217:Rheumatoid Factor