Time-dependent inhibition of sucrase and isomaltase from rat small intestine by castanospermine.
Castanospermine (1,6,7,8-tetrahydroxyoctahydroindolizine) is a potent time-dependent inhibitor of the sucrase-isomaltase complex purified from rat small intestine, in vitro. First-order kinetics for the inactivation of sucrase and isomaltase by castanospermine were observed. Protection studies showed that castanospermine competes for the glucosyl subsite with the substrates of sucrase and isomaltase. The second-order rate constants (k1) for the association reaction between castanospermine and the protein complex were calculated to be 6.5 X 10(3) and 0.3 X 10(3) M-1 s-1 for sucrase and isomaltase, respectively. Only barely detectable reactivation of the inhibited isomaltase was detectable over 24 h, whereas about 30% reactivation of the inhibited sucrase was observed in 24 h (k2 = 3.6 X 10(-6) s-1). These results suggest that castanospermine functions as a transition-state analog that binds extremely tightly to sucrase and isomaltase.
Danzin
C
C
Merrell Dow Research Institute, Strasbourg Research Center, France.
Ehrhard
A
A
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D007212:Indolizines; D007421:Intestine, Small / Q000201:enzymology*; D007700:Kinetics; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors*; D051381:Rats; D013394:Sucrase-Isomaltase Complex / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D013997:Time Factors; D054836:Indolizidines
D000470:Alkaloids; D007212:Indolizines; D009097:Multienzyme Complexes; D013394:Sucrase-Isomaltase Complex; C037806:castanospermine; D054836:Indolizidines