title: Specificity of non-histone protein antigens in chicken liver nuclei.
abstract: 1. Chicken liver nuclei were fractionated by disruption with ultrasound and subsequent precipitation with divatent cations. A small, protein-rich fraction (CS), representing less than 5% of the total nuclear DNA reacted strongly with antisera to dehistonized chicken liver chromatin. 2. Further fractionation of the CS by DNA-affinity chromatography yielded two DNA-binding immunoreactive proteins (approximate Mr 35 and 56 kD). 3. We have shown previously (Kiliańska et al., 1981, Kiliańska, 1984) that this DNA-binding component strongly inhibited the in vitro transcription of RNA.
authors: Department of Cytobiochemistry, University of Lódź, Poland.
authors: Z
authors: Z
authors: Kiliańska
authors: R C
authors: RC
authors: Briggs
authors: L S
authors: LS
authors: Hnilica
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D002467:Cell Nucleus / Q000276:immunology; D002645:Chickens; D002843:Chromatin / Q000276:immunology; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000276:immunology*
chemical_list: D000941:Antigens; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone