Crosslinked concanavalin A-O-(diethylaminoethyl)-cellulose--an affinity medium for concanavalin A-interacting glycoproteins.
When concanavalin A (Con A) is reacted with a low concentration of glutaraldehyde, the product formed strongly binds to DEAE-cellulose. Thus, the resultant material can be used as an affinity medium for those glycoproteins which interact with Con A. This affinity medium is easy to prepare, has a capacity comparable to that of similar commercially available affinity media, and is stable for up to at least 6 months.
Kamra
A
A
Chemistry Department, Indian Institute of Technology, New Delhi.
Gupta
M N
MN
D000135:Acid Phosphatase / Q000302:isolation & purification; D000469:Alkaline Phosphatase / Q000302:isolation & purification; D002482:Cellulose / Q000031:analogs & derivatives*; D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A / Q000031:analogs & derivatives*; D003636:DEAE-Cellulose / Q000031:analogs & derivatives*; D006023:Glycoproteins / Q000302:isolation & purification*; D011952:Receptors, Concanavalin A / Q000302:isolation & purification; D012260:Ribonucleases / Q000302:isolation & purification
D006023:Glycoproteins; D011952:Receptors, Concanavalin A; C053983:concanavalin A-O-(diethylaminoethyl)cellulose; C027774:concanavalin A-binding glycoproteins; D003208:Concanavalin A; D002482:Cellulose; D003636:DEAE-Cellulose; D012260:Ribonucleases; C029600:ribonuclease B; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase