The common structure and activities of four subspecies of rat brain protein kinase C family.
Elucidation of the complete sequences of four cDNA clones (alpha, beta I, beta II, and gamma) of the rat brain protein kinase C family has revealed their common structure composed of a single polypeptide chain with four constant (C1-C4) and five variable (V1-V5) regions. Although these sequences are highly homologous and closely related to one another V3-, V4-, and V5-regions of gamma-subspecies are slightly bigger than the corresponding regions of the other three subspecies. The first constant region, C1, contains a tandem repeat of cysteine-rich sequence (6, total 12 cysteine residues). The third constant region, C3, has an ATP-binding sequence which is found in many protein kinases. In adult rat whole brain, the relative activities of alpha-, beta I-, beta II-, and gamma-subspecies are roughly 16, 8, 55, and 21%, respectively. gamma-Subspecies is expressed after birth apparently only in the central nervous tissue, implying its role in the regulation of specific neuronal functions.
Kikkawa
U
U
Department of Biochemistry, Kobe University School of Medicine, Japan.
Ogita
K
K
Ono
Y
Y
Asaoka
Y
Y
Shearman
M S
MS
Fujii
T
T
Ase
K
K
Sekiguchi
K
K
Igarashi
K
K
Nishizuka
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D011493:Protein Kinase C / Q000145:classification*; D051381:Rats; D013329:Structure-Activity Relationship
D011493:Protein Kinase C