title: Chemical structures of a galactose-rich glycoprotein of Leishmania tarentolae.
abstract: 1. Aqueous phenol treatment of water extracted disrupted cells of Leishmania tarentolae (LV-414) provided a glycoprotein mixture which was purified by gel filtration chromatography, and Concanavalin A-Sepharose column. 2. The bound fraction on Concanavalin A-Sepharose column (protein 74%, and carbohydrate, 26%) had [alpha]D + 9 degrees and contained mannose (18%), galactose (60%), and glucose (22%), and some of the galactose residues were resistant to periodate oxidation. 3. Treatment of the phenol extract with hot aqueous NaBH4 containing NaOH gave a preparation having mannose (12%), galactose (82%), and glucose (6%). 4. Methylation analysis showed the presence of a mainly linear structure with non-reducing end-units of mannopyranose (6%), 3-O-substituted galactopyranosyl (64%), 2-O- (11%), and 6-O- (5%) substituted mannopyranosyl, and 4-O- (9%), and 4,6-di-O- (3%) substituted glycopyranosyl units. 5. The specific rotation of the preparation, +20 degrees, indicated beta-linked galactopyranosyl units.
authors: Departamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro, Brasil.
authors: M T
authors: MT
authors: Xavier
authors: J O
authors: JO
authors: Previato
authors: P A
authors: PA
authors: Gorin
authors: L
authors: L
authors: Mendonça-Previato
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D007891:Leishmania / Q000032:analysis*; D008970:Molecular Weight
chemical_list: D002241:Carbohydrates; D006023:Glycoproteins