title: Ancymidol blocks trichothecene biosynthesis and leads to accumulation of trichodiene in Fusarium sporotrichioides and Gibberella pulicaris.
abstract: Ancymidol, a plant growth regulator, inhibited biosynthesis of diacetoxyscirpenol by Gibberella pulicaris (Fusarium sambucinum) in a defined liquid medium. Ancymidol also inhibited biosynthesis of T-2 toxin by a wild-type strain of Fusarium sporotrichioides and biosynthesis of diacetoxyscirpenol, deacetylated calonectrin, and dideacetylated calonectrin by mutant strains of this species. Ancymidol-treated cultures accumulated the hydrocarbon trichodiene, a biosynthetic precursor of the trichothecenes. Ancymidol did not block trichodiene accumulation by a trichodiene-producing mutant strain of F. sporotrichioides. Ancymidol appears to block the trichothecene biosynthetic pathway after formation of trichodiene and before formation of trichothecenes containing four or more oxygen atoms.
authors: Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Illinois 61604.
authors: A E
authors: AE
authors: Desjardins
authors: R D
authors: RD
authors: Plattner
authors: M N
authors: MN
authors: Beremand
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D003470:Culture Media; D053138:Cyclohexenes; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D009183:Mycotoxins / Q000096:biosynthesis; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000096:biosynthesis*; D013605:T-2 Toxin / Q000096:biosynthesis; D014255:Trichothecenes / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D053138:Cyclohexenes; D009183:Mycotoxins; D010937:Plant Growth Regulators; D011743:Pyrimidines; D012717:Sesquiterpenes; D014255:Trichothecenes; C053577:ancymidol; C012075:trichodiene; D013605:T-2 Toxin; C001809:diacetoxyscirpenol