title: New method for formulation of amino acid concentrations and ratios in diets of rats.
abstract: Parameters derived from the application of the saturation kinetics model (SKM) to rat growth experiments were used to formulate a complete dietary amino acid mix for weanling rats. Dietary ratios and concentrations for indispensable amino acids (IAA) and arginine were calculated using the parameter K0.5. A curve shift technique was used to determine dietary concentrations for conditionally indispensable amino acids (CAA) and dispensable amino acids (DAA). The model was also used to determine a dietary ratio of (IAA + Arg)/(CAA + DAA). Using the dietary amino acid concentrations suggested by the model and an (IAA + Arg)/(CAA + DAA) ratio of 1, a growth response curve was constructed and compared to a similar curve using the amino acid mix of Rogers and Harper. The modeling approach produced a 10-15% improvement in growth over the Rogers and Harper mix. The SKM is discussed in terms of calculating an ideal nutrient ratio and choosing a desired response level. It is demonstrated that the model can rapidly produce accurate estimates for dietary amino acid levels, while minimizing required numbers of laboratory animals.
authors: Department of Biochemistry, School of Medicine, Oral Roberts University, Tulsa, OK 74171.
authors: L P
authors: LP
authors: Mercer
authors: S J
authors: SJ
authors: Dodds
authors: D L
authors: DL
authors: Smith
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage* / Q000494:pharmacology / Q000502:physiology; D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004435:Eating; D007700:Kinetics; D008297:Male; D008433:Mathematics; D008954:Models, Biological; D009751:Nutritional Requirements; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000596:Amino Acids; D001120:Arginine