A novel tetracycline from Actinomadura brunnea. Fermentation, isolation and structure elucidation.
A novel tetracycline antibiotic, Sch 33256, was isolated from a culture broth of a new species of Actinomadura. The antibiotic was isolated by solvent extraction, Sephadex G-25 column chromatography and crystallization. The structure was determined by comparison of the spectra with that of chlortetracycline. Spectroscopic analysis of the compound yielded 2'-N-methyl-8-methoxychlortetracycline as the proposed structure.
Patel
M
M
Department of Microbial Products, Schering Corporation, Bloomfield, NJ 07003.
Gullo
V P
VP
Hegde
V R
VR
Horan
A C
AC
Gentile
F
F
Marquez
J A
JA
Miller
G H
GH
Puar
M S
MS
Waitz
J A
JA
D001419:Bacteria / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D002751:Chlortetracycline / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D005285:Fermentation; D009618:Nocardiaceae / Q000378:metabolism*
C054352:2'-N-methyl-8-methoxychlortetracycline; D002751:Chlortetracycline