One-dimensional and two-dimensional nuclear magnetic resonance studies of the reaction of phenyldichloroarsine with glutathione.
14C-labeled phenyldichloroarsine (PDA) enters the red blood cell and forms a 1:2 adduct with intracellular glutathione. Upon gel filtration of the hemolysate, [14C]PDA was recovered with the glutathione-containing fractions. One-dimensional and two-dimensional nuclear magnetic resonance spectroscopy were used to confirm the structure of the adduct and elucidate its stereochemistry, stability, and reactivity.
Dill
K
K
Department of Chemistry, Clemson University, South Carolina 29634-1905.
Adams
E R
ER
O'Connor
R J
RJ
Chong
S
S
McGown
E L
EL
D001152:Arsenicals / Q000097:blood*; D002250:Carbon Radioisotopes; D002850:Chromatography, Gel; D005978:Glutathione / Q000097:blood*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation
D001152:Arsenicals; D002250:Carbon Radioisotopes; C053663:phenyldichloroarsine; D005978:Glutathione