title: Bioenergetics in the aging Fischer 344 rat: effects of exercise and food restriction.
abstract: The capacity for energy production was evaluated in male, Fischer 344 rats as they advanced from adulthood through senescence. At 10 months of age, the animals were divided into three groups: sedentary, fed ad libitum (S); exercised by treadmill running, fed ad libitum (E); and sedentary, caloric restricted by alternate day feeding (R). Activities of selected enzymes, ADP-stimulated respiration and levels of cytochromes, were determined in homogenates of liver and gastrocnemius muscle prepared from young controls (10-month old S) and 18-, 24-, and 30-month old animals. In liver, age-linked decrements were found in the activities of 3-hydroxyacyl-CoA dehydrogenase (S, E, and R) and citrate synthase (S), and in cytochrome c content (S and E), whereas substrate-catalysed oxidations were unaffected. In the gastrocnemius muscle (S, E, and R), respiration, activities of enzymes of the Krebs cycle and glycolysis, and cytochrome content were decreased after the age of 18 months. Oxidative capacity was increased in muscle through exercise (about 40%) and in liver by food restriction (about 20%). Body and soleus muscle mass declined similarly in all groups (about 14% from 30 to 18 months of age), whereas the loss of weight in the gastrocnemius muscle was much greater (34%). The data indicate that energy metabolism in the senescent animal is competent to meet its needs and age-related declines in energy metabolism are secondary to the aging process.
authors: Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors: W L
authors: WL
authors: Rumsey
authors: Z V
authors: ZV
authors: Kendrick
authors: J W
authors: JW
authors: Starnes
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D003580:Cytochromes / Q000378:metabolism; D004734:Energy Metabolism; D005508:Food Deprivation / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000033:anatomy & histology / Q000201:enzymology; D010084:Oxidation-Reduction; D005082:Physical Exertion; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D003580:Cytochromes