Concentration dependence of the binding constant of antibodies.
By simultaneous dilution of the antigen (AG) and the antibody (AB) used in an immune precipitation reaction, at the equivalence AG/AB ratio, it was found that the association constant, Ka, increased from 1.7 X 10(7) 1/M at the highest concn of AG and AB, to 6.5 X 10(11) 1/M at 100-fold dilution of both. An important (but not the only) reason for this increase is taken to be the fact that the AB solution was polyclonal.
van Oss
C J
CJ
Department of Microbiology, State University of New York, Buffalo 14214.
Walker
J
J
D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D004306:Dose-Response Relationship, Immunologic; D007074:Immunoglobulin G / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine