Renal ammonia production from the nitrogens of glutamine in intact acidotic dogs before and after bicarbonate infusions.
Renal ammonia is produced from the amide nitrogen of glutamine, approximately 33-50%. The remainder derives from the amino nitrogen of glutamine and other non amide sources, probably the amino nitrogens of other amino acids. We investigated the acute effects of acid-base perturbations on ammonia production from amide and non amide nitrogen sources to determine how they interrelate. Infusions of glutamine were given to some intact dogs to vary the renal load. Following an acute alkali challenge to dogs in metabolic acidosis, ammoniagenesis from the amide nitrogens decreased significantly when the presentation of glutamine to the kidney was normal or relatively low, but changed less or even increased when the glutamine load was relatively high. In contrast, ammonia from the non amide sources consistently decreased during acute alkalotic challenge at any glutamine load-high or low. Since decreased glutamine deamination leading to glutamate accumulation is generally associated with decreased deamidation in dogs with normal plasma glutamine concentrations, we explain the discrepancy of deamidation at high glutamine loads to an unmasking of a separate effect on the glutaminase (phosphate-dependent) pathway by the acute acid-base changes. Accordingly, our results indicate more than one influence from acute acid-base changes in vivo on renal ammonia formation, one stimulatory and other inhibitory. Nevertheless, the influence of glutamate removal predominates over the other effect on the phosphate-dependent glutaminase pathway at physiological concentrations of glutamine in the intact dog.
Preuss
H G
HG
Department of Medicine (Nephrology), Georgetown University Medical Center, Washington, DC.
Slemmer
D
D
Areas
J
J
Conrado
J
J
D000138:Acidosis / Q000378:metabolism*; D000577:Amides / Q000378:metabolism; D000641:Ammonia / Q000652:urine*; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D004285:Dogs; D005260:Female; D005919:Glomerular Filtration Rate; D005973:Glutamine / Q000378:metabolism*; D007262:Infusions, Intravenous; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009584:Nitrogen / Q000378:metabolism*
D000577:Amides; D001639:Bicarbonates; D005973:Glutamine; D000641:Ammonia; D009584:Nitrogen