Measurement of the alpha 2 component of a novel oncodevelopmental antigen by a rapid electroimmunoassay.
A method was required for the quantification of a novel oncodevelopmental antigen which increases in the serum of pregnant women as well as patients with ovarian cancer. The native antigen is composed of two components having alpha 2 and beta electrophoretic mobilities. An assay method, involving electroimmunodiffusion according to Laurell, was developed for the measurement of the alpha 2 component of the antigen. The method is sufficiently sensitive to determine significant differences in the levels of antigen in the serum of non-pregnant and pregnant subjects, and patients with ovarian cancer. In addition to routine quantitation, the technique will also be an indispensable analytical tool in the purification of the protein.
Nakai
M
M
Department of Obstetrics and Gynaecology, Kitasato University School of Medicine, Kanagawa, Japan.
Atsumi
M
M
Odagiri
J
J
Arai
M
M
D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D010051:Ovarian Neoplasms / Q000276:immunology*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*
D000951:Antigens, Neoplasm; D011257:Pregnancy Proteins