Subnuclear associations of the v-myb oncogene product and actin are dependent on ionic strength during nuclear isolation.
The method used to isolate nuclei has a direct effect on the subnuclear association of the v-myb product, p48v-myb, and nuclear actin. Analysis of nuclei subjected to various isolation procedures showed that disruption of native nuclear structure during hypotonic treatment resulted in dissociation of p48v-myb from the nuclear matrix.
Boyle
W J
WJ
Department of Pathology, School of Medicine, University of California, Los Angeles 90024.
Baluda
M A
MA
D000199:Actins / Q000378:metabolism*; D000818:Animals; D009189:Avian Myeloblastosis Virus; D002451:Cell Compartmentation; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D009994:Osmolar Concentration; D012995:Solubility; D013696:Temperature
D000199:Actins; D009856:Oncogene Proteins, Viral