title: Determination of selenium in human spermatozoa and prostasomes using base digestion and electrothermal atomic absorption spectrophotometry.
abstract: A method for the determination of selenium in human spermatozoa and prostasomes is described. The samples were digested with 25% (w/v) tetramethylammonium hydroxide (TMAH) in methanol and analyzed by atomic absorption spectrometry with electrothermal atomization and Zeeman background correction (ET-AAS). Nickel was used as a matrix modifier. Calibration was performed using the matrix-based calibration curve. The TMAH-digestion method agreed well with a conventional digestion procedure using concentrated nitric acid. The TMAH-digestion does not require heating or strong acids and it was suitable for small biological samples. The average recovery of added selenium in spermatozoan digests was 95.1 +/- 5.2% (n = 5). The coefficient of variation was 9.1% (n = 21). The accuracy of the method tested with the NBS standard 1577 (bovine liver, certified at 1.1 +/- 0.1 micrograms Se/g) resulted in a value of 0.98 +/- 0.10 micrograms Se/g (n = 16). The method was further tested in an interlaboratory comparison study.
authors: Department of Chemistry, University of Kuopio, Finland.
authors: U
authors: U
authors: Suistomaa
authors: M
authors: M
authors: Saaranen
authors: T
authors: T
authors: Vanha-Perttula
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis; D005399:Fishes; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D009206:Myocardium / Q000032:analysis; D012643:Selenium / Q000032:analysis* / Q000097:blood; D013045:Species Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods; D013094:Spermatozoa / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D012643:Selenium