Vitamin D and its metabolites in the pollen of pine. Part 5: Steroid hormones in the pollen of pine species.
Unconjugated vitamin D and its metabolites were investigated in the pollen of Pinus nigra Ar. and Pinus sylvestris L. by TLC, HPLC and competitive radiochemical determination of 25-hydroxycholecalciferol (25-OHD3). It was found that vitamin D (D2, D3) was present in the pollen in amounts about 2 micrograms/10 g and 25-OHD3, 24,25-dihydroxycholecalciferol [24,25-(OH)2D3] and 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] between 0.1 and 3 micrograms/10 g of pollen, dependent on pollen species and methods.
Saden-Krehula
M
M
Department of Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Yugoslavia.
Tajíć
M
M
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D010944:Plants / Q000032:analysis*; D011058:Pollen / Q000032:analysis*; D011485:Protein Binding; D013256:Steroids / Q000032:analysis*; D014807:Vitamin D / Q000032:analysis*
D013256:Steroids; D014807:Vitamin D