title: Identification of two human phosphoproteins (dividin and IEF 59dl) that are first detected late in G1 near the G1/S transition border of the cell cycle.
abstract: Two-dimensional gel electrophoretic analysis (NEPHGE, IEF) of the [32P]-orthophosphate-labeled proteins synthesized throughout the cell cycle of transformed human amnion cells (AMA) revealed two phosphoproteins (dividin, Mr = 54,000, pl = 8.4; IEF 59dl, Mr = 27,000, pl = 5.7) that are present mainly in S-phase cells. These proteins are first detected at the end of G1, near the G1/S transition border, and their levels reach a maximum late in S-phase. Together with the previously identified nuclear protein cyclin, these phosphoproteins are likely candidates for proteins that may play a role in the regulation of the onset of DNA synthesis and cell division.
authors: Department of Medical Biochemistry, Aarhus University, Denmark.
authors: S
authors: S
authors: Nielsen
authors: A
authors: A
authors: Celis
authors: G P
authors: GP
authors: Ratz
authors: J E
authors: JE
authors: Celis
mesh_terms: D000650:Amnion; D002453:Cell Cycle; D002461:Cell Line, Transformed; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007399:Interphase; D007526:Isoelectric Point; D008938:Mitosis; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism*
chemical_list: D010750:Phosphoproteins; C053990:dividin