Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method.
Chemical ionization- and electron impact ionization-selective ion monitoring provided a simple and sensitive method for measuring detomidine (Domosedan), a potent sedative-analgesic drug for horses and cattle. Chemical ionization was at least 10 times more sensitive than electron impact ionization. By using propranolol as an internal standard, we found that the recovery of detomidine from the extraction procedure used in this study was greater than 75% for plasma, whole blood, or urine samples. Approximately 68% of detomidine was bound to plasma protein and 53% was bound to red blood cells.
Singh
A K
AK
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Mishra
U
U
Ashraf
M
M
Abdennebi
E H
EH
Granley
K
K
Dombrovskis
D
D
Hewetson
D
D
Stowe
C M
CM
D000700:Analgesics / Q000032:analysis* / Q000097:blood / Q000652:urine; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004912:Erythrocytes / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses; D007093:Imidazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D011485:Protein Binding
D000700:Analgesics; D001798:Blood Proteins; D007093:Imidazoles; C041255:detomidine