[Identification and characteristics of mRNA's showing increased levels during DNA replication].
A library of double-stranded cDNA was prepared using poly (A) + RNA from regenerating rat liver 20 h after partial hepatectomy. Differential screening of 350 recombinant clones with cDNA-G0 and cDNA-S identified eleven cDNA clones (pRL), the sequences of which were preferentially expressed during the DNA replication period. Levels of mRNAs complementary to these clones were 2--10-fold higher in the S-period, than in G0. Using plasmid cDNAs to different mRNA, pRL we have investigated the changes in the levels of mRNA pRL during liver regeneration. The level of mRNA mRL2 and pRL79 was increased just before DNA replication. mRNA pRL35 accumulates after partial hepatectomy with the maximum at 6 h. The augment of two other mRNA concentrations was expressed to a lesser extent. Northern-blot analysis allowed to determine the individual dual mRNAs corresponding to each of the three clones with their sizes ranging from about 1650 to 3900 bases. Three mRNAs (pRL35, 67 and 79) were shown (by hybrid-selected translation) to code for proteins of about 100, 140 and 120 kDa, respectively.
Timchenko
N A
NA
Timchenko
L T
LT
Bilobrov
S V
SV
Zhuchenko
O P
OP
Krutiakov
V M
VM
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004261:DNA Replication; D008099:Liver / Q000032:analysis; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats; D013698:Templates, Genetic
D012333:RNA, Messenger; D004247:DNA
Identifikatsiia i kharakteristika mRNK, soderzhanie kotorykh povysheno v period replikatsii DNK.