Identification and quantification of 5-hydroxypipecolic acid and hydroxyproline in mammalian brain and blood by selected ion monitoring.
A method for the analysis of 5-hydroxypipecolic acid and 4-hydroxyproline in mammalian brain and blood is reported. The identification and quantification of the two hydroxyimino acids were accomplished with gas chromatography/mass spectrometry including a selected ion-monitoring technique following HPLC prepurification. The lower limit of detection for the method is 2 to 10 pmol. The amounts of 5-hydroxypipecolic acid and 4-hydroxyproline in blood were 20 to 30 pmol/ml and 3 to 6 nmol/ml, respectively. Their concentrations in the rabbit whole brain were determined to be 5 and 120 pmol/g, respectively.
Miyata
T
T
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Okano
Y
Y
Nagata-Tanoue
J
J
Ijima-Miyamura
S
S
Iwamura
H
H
Takahama
K
K
Hitoshi
T
T
D000328:Adult; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis* / Q000097:blood; D008297:Male; D010875:Pipecolic Acids / Q000032:analysis* / Q000097:blood; D011817:Rabbits
D010875:Pipecolic Acids; C053574:5-hydroxypipecolic acid; D006909:Hydroxyproline