title: Determination of hippurate hydrolysis by gas-liquid chromatography.
abstract: A rapid gas-liquid chromatographic procedure was developed to determine hippurate hydrolysis by microorganisms. Bacterial cells were inoculated into 0.4 ml of 1% sodium hippurate and incubated for 2 h at 37 degrees C. Cells were removed by centrifugation, and the benzoate released by enzyme activity was converted to methyl benzoate and analyzed by gas-liquid chromatography. This procedure is sensitive, and its specificity provides a high degree of reliability for organisms with weak hippuricase activity.
authors: Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
authors: P L
authors: PL
authors: Wallace
authors: C M
authors: CM
authors: Patton
authors: C W
authors: CW
authors: Moss
mesh_terms: D001565:Benzoates / Q000032:analysis*; D002167:Campylobacter / Q000378:metabolism*; D002168:Campylobacter fetus / Q000378:metabolism*; D002849:Chromatography, Gas; D006626:Hippurates / Q000378:metabolism*; D006868:Hydrolysis; D007875:Legionella / Q000378:metabolism*; D011237:Predictive Value of Tests
chemical_list: D001565:Benzoates; D006626:Hippurates