Secretion of plasminogen activator by cerebral astrocytes and its modulation.
Plasminogen activator (PA) has been related to the neuron migration during brain development. PA has also been shown to degrade myelin basic protein. We present data to show that neonatal Balb/c astrocytes show PA activity on 125I-fibrin coated plates. Secreted and cell associated fibrinolytic activity is detected only in the presence of plasminogen. Modulants like concanavalin A and phorbol myristate acetate enhance PA production and this function involves a transcriptional event. Dexamethasone inhibits baseline as well as concanavalin A induced enhancement of PA activity. These results raise the possibility that astrocytes may have an active role in myelinoclastic disorders and CNS developmental defects.
Toshniwal
P K
PK
Department of Neurology, Cook County Hospital, Chicago, IL 60612.
Tiku
M L
ML
Tiku
K
K
Skosey
J L
JL
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010960:Plasminogen Activators / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003208:Concanavalin A; D003609:Dactinomycin; D003907:Dexamethasone; D003513:Cycloheximide; D010960:Plasminogen Activators; D013755:Tetradecanoylphorbol Acetate