[DNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization].
A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.
Adarichev
V A
VA
Dymshits
G M
GM
Kalachikov
S M
SM
Pozdniakov
P I
PI
Salganik
R I
RI
D002082:Butylamines; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes; D009693:Nucleic Acid Hybridization
D002082:Butylamines; D005456:Fluorescent Dyes; C047901:1-aminooxy-4-aminobutane; D004247:DNA
Izuchenie DNK, nesushcheĭ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoĭ gibridizatsii.