Studies on the neuromuscular junction with a special preference to nicotinic receptors in normal delivering cows and cows suffering from parturient paresis.
Acetylcholine synthesizing (ACh-s) activity, and binding of 3H-alpha-bungarotoxin (3H-Btx) and 3H-d-tubocurarine (3H-TC) were analysed in gluteus media muscle from normal cows and from cows suffering from parturient paresis, both at parturition and 3 months later. At parturition, the ACh-s activity was 50% lower in the muscle of paretic cows than in non-paretic cows. No difference was found in the number of 3H-Btx and 3H-TC binding sites at parturition, while 3 months after parturition the numbers of 3H-TC and 3H-Btx binding sites were significantly higher in normal than in paretic cows. These significant differences in the number of receptors and a possible underlying receptor regulation defect might be a plausible etiological factor for the disease parturient paresis.
Forslund
K
K
Department of Cattle and Sheep Diseases, College of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Nordberg
A
A
D000109:Acetylcholine / Q000096:biosynthesis; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002118:Calcium / Q000097:blood; D002417:Cattle / Q000378:metabolism*; D002418:Cattle Diseases / Q000097:blood / Q000201:enzymology / Q000378:metabolism*; D002802:Cholinesterases / Q000097:blood; D005260:Female; D009132:Muscles / Q000378:metabolism; D009469:Neuromuscular Junction / Q000378:metabolism*; D010319:Parturient Paresis / Q000097:blood / Q000201:enzymology / Q000378:metabolism*; D010758:Phosphorus / Q000097:blood; D011247:Pregnancy; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014403:Tubocurarine / Q000378:metabolism
D002038:Bungarotoxins; D011978:Receptors, Nicotinic; D010758:Phosphorus; D002802:Cholinesterases; D000109:Acetylcholine; D002118:Calcium; D014403:Tubocurarine