[Effect of temperature on the conformation of native DNA in aqueous solutions of various electrolytes].
Effect of the temperature on the conformation of the native DNA molecule in solution of different electrolytes (LiCl, NaCl, KCl, CsCl, Gu-HCl) at ionic strengths mu = 5; 0.1; 0.01; 0.005 and temperatures ranging from 10 to 40 degrees C were studied by the methods of flow birefringence and viscometry. The experiments showed that the value of intrinsic viscosity [eta] of DNA increases at increase of temperatures in solutions of all the chlorides studied, excluding guanidine. The effect of temperature on the value of [eta] doesn't depend on the type of the cation at a fixed value of mu and is elevated when mu decreases. The observed alterations of the value of [eta] for DNA in water-salt solutions at different temperatures can be explained by an increase in the hydration of the alkaline ions at temperature increase. The experiments showed the specificity of the effect of different ions on the dimensions of the DNA molecule in solution. The data on optical anisotropy of the DNA molecule testify, that the thermodynamic rigidity of the latter doesn't depend on the temperature of solutions of different electrolytes in the temperature range studied.
Sibileva
M A
MA
Veselkov
A N
AN
Shilov
S V
SV
Frisman
E V
EV
D000818:Animals; D002417:Cattle; D004247:DNA; D004573:Electrolytes; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D012996:Solutions; D013696:Temperature; D013950:Thymus Gland; D014783:Viscosity
D004573:Electrolytes; D012996:Solutions; D004247:DNA
Vliianie temperatury na konformatsiiu molekuly nativnoĭ DNK v vodnykh rastvorakh razlichnykh élektrolitov.