Effect of different fractions of dialyzable leukocyte extract--transfer factor--on the chemiluminescence of human phagocytes.
The chemiluminescence (CL) inducing effect of the fractions of dialyzable leukocyte extract (DLE)--transfer factor--from tonsil leukocytes was tested on the phagocytes of human whole blood. The fractionation of DLE by Sephadex G-25 results in three main fractions: I, II, III. We found only fraction II having a direct CL inducing effect on the non-stimulated cells. Fraction II increased further the CL of phagocytes which were stimulated by zymosan (Mannozym). Fractions I and III were ineffective. We suggest that in the complex "transfer factor" effects of DLE, the stimulation of the peripheral phagocytes of blood-by the oligopeptides of fraction II may have an important role.
Sipka
S
S
3rd Department of Medicine, University Medical School, Debrecen, Hungary.
Schröder
I
I
Brazsnikova
N A
NA
D006801:Humans; D008163:Luminescent Measurements; D010586:Phagocytes / Q000187:drug effects*; D014165:Transfer Factor / Q000031:analogs & derivatives / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology
D014165:Transfer Factor; D015054:Zymosan