Activation of the human c-Ha-ras-1 proto-oncogene by in-vitro reaction with N-nitrosomethyl(acetoxymethyl)amine.
Reaction of N-nitrosomethyl(acetoxymethyl)amine in vitro in the presence of esterase with a plasmid containing the human c-Ha-ras-1 gene has been shown to generate a transforming oncogene when the methylated DNA is transfected into NIH 3T3 cells. Our results show that an activated nitrosamine can mutate a normal cellular proto-oncogene, a reaction that may be the necessary first step in the multistage process of tumour induction.
Hirani-Hojatti
S
S
Department of Medical Biophysics, University of Toronto, Ontario, Canada.
Milligan
J R
JR
Archer
M C
MC
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D004247:DNA / Q000187:drug effects*; D004128:Dimethylnitrosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008745:Methylation; D051379:Mice; D008808:Mice, Inbred CBA; D010957:Plasmids; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D014162:Transfection
D002273:Carcinogens; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; C014659:methyl(acetoxymethyl)nitrosamine; D004247:DNA; D004128:Dimethylnitrosamine