title: Long-term effects of estrogen on avian liver: estrogen-inducible switch in expression of nuclear, hormone-binding proteins.
abstract: The stimulation of chicks or embryos with estrogen results in transient, hepatic expression of the vitellogenin gene, as well as long-term, propagatable alterations in the rapidity with which the gene can be reactivated. We examined the possibility that nuclear, type II estrogen-binding sites are involved in this long-term change in response characteristics. We demonstrate that the primary induction kinetics of type II sites in embryos and chicks correlated with the expression of the vitellogenin gene and that once their induction was triggered by estrogen, they accumulated, were propagated, and persisted for months after withdrawal of the hormone. We also show that their accumulation in the embryo was accompanied by prolonged expression of both the vitellogenin and very low-density apolipoprotein II genes, in the absence of elevated levels of type I receptor, and that the type II sites, like the classical receptor, appear to be preferentially associated with active or potentially active chromatin. Finally, we describe a regulatory mechanism, tested by computer modelling, that simulated the behavioral characteristics of these nuclear estrogen-binding sites and which may explain their role in mediating the long-term effects of estrogen.
authors: Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
authors: R J
authors: RJ
authors: Haché
authors: S P
authors: SP
authors: Tam
authors: A
authors: A
authors: Cochrane
authors: M
authors: M
authors: Nesheim
authors: R G
authors: RG
authors: Deeley
mesh_terms: D000367:Age Factors; D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D002642:Chick Embryo; D003850:Deoxyribonuclease I; D004958:Estradiol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008079:Lipoproteins, VLDL / Q000235:genetics; D008099:Liver / Q000502:physiology; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000145:classification / Q000378:metabolism*; D014158:Transcription, Genetic; D014819:Vitellogenins / Q000235:genetics
chemical_list: D008079:Lipoproteins, VLDL; D009687:Nuclear Proteins; D011960:Receptors, Estrogen; D014819:Vitellogenins; D004958:Estradiol; D003850:Deoxyribonuclease I