A water-soluble, monitorable peptide and protein crosslinking agent.
A novel, freely water-soluble, heterobifunctional crosslinking reagent, N-maleimido-6-aminocaproyl ester of 1-hydroxy-2-nitro-4-benzenesulfonic acid (mal-sac-HNSA), was synthesized and used for conjugation of sulfhydryl (cysteine)-containing peptides to carrier proteins. Reaction with amino groups releases the dianion phenolate, HNSA, which allows convenient spectrophotometric quantitation of the reaction in progress. Since mal-sac-HNSA is completely water soluble, its concentration can be adjusted to maximize the rate of amine reaction and to minimize hydrolysis. Conjugates of peptides to appropriate carriers have elicited peptide-specific antibody and did not elicit detectable antibody specific to the crosslink.
Aldwin
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Cetus Corporation, Emeryville, California 94608.
Nitecki
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DE
D001557:Benzenesulfonates / Q000138:chemical synthesis; D003432:Cross-Linking Reagents / Q000138:chemical synthesis; D008301:Maleimides / Q000138:chemical synthesis; D010455:Peptides; D011506:Proteins; D012995:Solubility; D014867:Water
D001557:Benzenesulfonates; D003432:Cross-Linking Reagents; D008301:Maleimides; D010455:Peptides; D011506:Proteins; D014867:Water; C053984:N-maleimido-6-aminocaproyl 1-hydroxy-2-nitro-4-benzenesulfonic acid ester