Cloning of whiG, a gene critical for sporulation of Streptomyces coelicolor A3(2).
In whiG mutants of Streptomyces coelicolor A3(2), aerial hyphae do not show any sign of sporulation. A library of S. coelicolor DNA was prepared in a phi C31 temperate phage vector (KC516), and one recombinant phage (KC750) that could restore the wild-type phenotype to a collection of whiG mutants when integrated into their genomes was found. Subcloning experiments with low- and high-copy-number Streptomyces plasmid vectors allowed partial localization of whiG in the cloned DNA and revealed that hypersporulation was associated with the presence of extra copies of whiG.
Mendez
C
C
John Innes Institute, Norwich, England.
Chater
K F
KF
D003001:Cloning, Molecular; D003090:Coliphages; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D009154:Mutation; D010957:Plasmids; D013171:Spores, Bacterial; D013302:Streptomyces / Q000235:genetics* / Q000502:physiology; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
D004269:DNA, Bacterial