[Effect of lipids on CO recombination with ferrocytochrome P-450].
Using the flash photolysis technique the kinetics of recombination of carbon monoxide with ferrocytochrome P-450 LM-2 was investigated. Ferrocytochrome P-450 was incorporated into liposomes prepared from different lipids: from microsomal lipids, phosphatidylcholine from egg yolk, dipalmitoylphosphatidylcholine. It was found that the activity and structure of ferrocytochrome P-450 conformers is affected by the lipid microenvironment. The kinetics of the CO-binding is affected also by the nature of lipids.
Khanina
O Iu
OIu
Uvarov
V Iu
VIu
Davydov
R M
RM
D002248:Carbon Monoxide / Q000032:analysis*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D005296:Ferrous Compounds / Q000032:analysis*; D007700:Kinetics; D008563:Membrane Lipids / Q000032:analysis*; D010084:Oxidation-Reduction; D011510:Proteolipids / Q000032:analysis*
D005296:Ferrous Compounds; D008563:Membrane Lipids; D011510:Proteolipids; C020485:proteoliposomes; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System
Vliianie lipidov na rekombinatsiiu CO s ferrotsitokhromom P-450.