Identification of the Yukb allo-antigen on platelet glycoprotein IIIa.
The glycoprotein (GP) localization of a new platelet-specific allo-antigen Yukb is described. The antibody was isolated from serum of a patient with neonatal allo-immune thrombocytopenia. In immunoblot procedure, it bound exclusively to platelet GP IIIa, like anti-PlA1, while the known anti-Baka and anti-Leka reacted with GP IIb. Analysis of GP from chymotrypsin-treated platelets with anti-Yukb revealed no binding in the 68-kilodalton position while anti-PlA1 did. Thus, unlike the PlA1 antigen, the Yukb determinant either resides on the 30-kilodalton fragment of GP IIIa or it is destroyed by chymotrypsin treatment.
Santoso
S
S
Institute of Clinical Immunology and Blood Transfusion, Justus Liebig University, Giessen, FRG.
Shibata
Y
Y
Kiefel
V
V
Mueller-Eckhardt
C
C
D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*
D007519:Isoantigens; D010980:Platelet Membrane Glycoproteins