Individual clones of hemopoietic cells in murine long-term bone marrow culture.
Forty-seven individual hemopoietic cell clones bearing unique radiation markers were studied in long-term bone marrow cultures. Throughout cultivation clones appeared at different times, from 1 to 12 weeks after explantation, survived during 1-10 more weeks, and were characterized by marked variability in size. Usually, the number of metaphases peculiar to an individual clone rapidly increased, achieved maximum, and then underwent a decline. Cells of reliably disappearing clones were never seen again. The experimental results provide further evidence for the model of hemopoiesis by clonal succession.
Chertkov
J L
JL
Central Institute of Hematology and Blood Transfusion, Moscow, U.S.S.R.
Deryugina
E I
EI
Drize
N J
NJ
Udalov
G A
GA
D000818:Animals; D001854:Bone Marrow Cells; D002453:Cell Cycle; D002478:Cells, Cultured; D002999:Clone Cells; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000528:radiation effects; D008677:Metaphase; D051379:Mice; D011839:Radiation, Ionizing