title: Investigation of the procoagulant activity present in ascitic fluid and serum of mice bearing the Landschütz ascites carcinoma.
abstract: The direct procoagulant activity (PCA) of murine tumour cells was found to be more than three orders of magnitude greater than an equivalent concentration of either resident or Corynebacterium parvum-elicited, exudate peritoneal cells. Similarly, a soluble PCA was detected in the extracellular culture medium of only the tumour cells. Studies on the procoagulant nature of the serum and ascitic fluid of tumour-bearing animals suggested that the ascitic fluid may contain a unique PCA factor(s). This activity could not, however, be resolved from inherent procoagulant factors, either by gel filtration or ammonium sulphate fractionation, and a more specific assay for, say, a single enzymic reaction in the coagulation cascade would be required to identify the tumour-associated activity.
authors: Department of Pathology, University of Aberdeen, Scotland, UK.
authors: L M
authors: LM
authors: Morrice
authors: L C
authors: LC
authors: McIntosh
authors: A W
authors: AW
authors: Thomson
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000201:enzymology* / Q000473:pathology; D001779:Blood Coagulation Factors / Q000302:isolation & purification*; D002277:Carcinoma / Q000097:blood / Q000201:enzymology*; D002850:Chromatography, Gel; D005260:Female; D051379:Mice; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D010529:Peritoneal Cavity / Q000473:pathology
chemical_list: D001779:Blood Coagulation Factors; D009363:Neoplasm Proteins; C045072:macrophage procoagulant activity