Spectropolarimetric determination of the guanine-cytosine content of DNA.
A circular dichroism technique for the determination of DNA base composition in terms of the guanine-cytosine content has been developed. A linear relationship between the ratio of ellipticities at 260 and 280 nm (theta 260/theta 280) and base composition of DNA is obtained in citrate-phosphate buffer, pH 3.0 at 20 degrees C. The DNA base composition obtained with this method is not only highly reproducible but is also the fastest method reported.
Maiti
M
M
Indian Institute of Chemical Biology, Calcutta.
Nandi
R
R
D001482:Base Composition; D002942:Circular Dichroism; D003596:Cytosine / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D006147:Guanine / Q000032:analysis*; D013057:Spectrum Analysis
D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine; D004247:DNA