Glycoconjugate biosynthesis in human epidermis.
Epidermal glycoconjugates synthesized by skin slices in media containing radiolabelled sugars were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A large proportion of the epidermal glycoconjugates were soluble in 10% (w/v) trichloroacetic acid. In addition to glycolipids and glycosaminoglycans, the acid-soluble fraction contained glycoproteins, a group of which (mol. wt. 70,000-160,000) were highly glycosylated and particularly rich in fucose. Analysis of the glycosaminoglycans by cellulose acetate electrophoresis combined with specific enzymic and nitrous acid degradation studies revealed that hyaluronic acid (73%) and heparan sulphate (18%) were the main components.
Roberts
G P
GP
Department of Medicine, University of Wales College of Medicine, Heath Park, Cardiff, U.K.
D002478:Cells, Cultured; D004588:Electrophoresis, Cellulose Acetate; D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000378:metabolism*; D006001:Glycoconjugates / Q000096:biosynthesis*; D006017:Glycolipids / Q000096:biosynthesis; D006023:Glycoproteins / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight
D006001:Glycoconjugates; D006017:Glycolipids; D006023:Glycoproteins; D006025:Glycosaminoglycans; D046911:Macromolecular Substances