Partitioning behaviour of rat bone marrow cells in aqueous two-phase systems. Dependence of cell partition on the interfacial tension and electrical potential difference between the phases.
The partitioning of heterogeneous cell populations of rat bone marrow, in comparison with that of homogeneous populations of erythrocytes, has been studied in aqueous two-phase systems containing increasing concentrations of dextran-poly-(ethylene glycol) (to increase the interfacial tension) and/or decreasing phosphate concentrations (to decrease both the electrical potential difference between the phases and the interfacial tension). At any polymer and/or phosphate concentration tested the partition is lower for bone marrow cells, reflecting their lower surface charge and hydrophobicity.
García-Pérez
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Departamento de Bioquimica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
Recio
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Sancho
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Luque
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D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D004563:Electrochemistry; D004912:Erythrocytes / Q000166:cytology; D008297:Male; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains; D013500:Surface Tension
D011092:Polyethylene Glycols