The influence of membrane composition on the solubilizing effects of Triton X-100.
Multilamellar liposomes containing pure phosphatidylcholine (PC) or mixtures of PC with cholesterol, cholesteryl palmitate, beta-carotene, cardiolipin, phosphatidylethanolamine or gramicidin A have been treated with the detergent Triton X-100. Solubilization has been monitored as a decrease in turbidity of the liposome suspension, and also by determination of bilayer components in the solubilized fraction. The same solubilization pattern is found for unsaturated (egg yolk) or saturated (dimyristoyl) PC. Similar results are also found when dimyristoyl PC is solubilized above or below its gel-to-fluid transition temperature. Cholesterol solubilizes in parallel with PC; gramicidin A is solubilized preferentially to this phospholipid and the non-polar lipids cholesteryl palmitate or beta-carotene remain insoluble at detergent concentrations producing complete PC solubilization. Addition of cardiolipin or phosphatidylethanolamine does not seem to alter the general pattern of PC solubilization. Phosphatidylethanolamine is less soluble than PC, while cardiolipin solubilizes at the same detergent concentrations than PC. These results are considered in relation to previous studies with natural membranes.
Urbaneja
M A
MA
Department of Biochemistry, Faculty of Science, University of the Basque Country, Bilbao, Spain.
Nieva
J L
JL
Goñi
F M
FM
Alonso
A
A
D002784:Cholesterol; D003902:Detergents; D007700:Kinetics; D008081:Liposomes; D017830:Octoxynol; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D012995:Solubility; D013501:Surface-Active Agents
D003902:Detergents; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; D002784:Cholesterol