A comparison by ultracentrifugation of the effects on DNA of ethidium bromide and of acridine orange at low ionic strength.
The application of scaled particle theory to the gels formed by DNA in the ultracentrifuge has provided values for the effective length and the effective radius of the DNA particle. Ethidium bromide has been shown to cause extensive lengthening of the DNA in dilute salt. Acridine orange interaction with DNA resulted in modest changes in DNA dimensions. These results are explained in terms of binding for acridine orange and of denaturation of DNA by ethidium bromide.
Richard
A J
AJ
Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond 23298-0001.
D000165:Acridine Orange / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000648:ultrastructure*; D004996:Ethidium / Q000494:pharmacology*; D009994:Osmolar Concentration; D014461:Ultracentrifugation / Q000379:methods
D004247:DNA; D004996:Ethidium; D000165:Acridine Orange