title: Differences in the distribution of F-actin in outer hair cells along the organ of Corti.
abstract: There is evidence of differences in the structure, innervation and physiological responses between outer hair cells (OHCs) of the basal and apical turns of the mammalian cochlea. In this study we have used rhodamine-labelled phalloidin to investigate the differential distribution of F-actin in OHCs along the organ of Corti of the guinea pig. Isolated OHCs and surface preparations and cryosections of the organ of Corti were studied. F-actin was observed in stereocilia and the cuticular plate of all OHCs. In addition, some OHCs had a network of F-actin extending from the cuticular plate towards the nucleus. This infracuticular network was observed in most OHCs of the apical cochlear turns but was not seen in any OHCs of the basal turn. These microstructural differences between OHCs of the base and apex could be related to differences in OHC function between the apical and basal portions of the cochlea.
authors: Kresge Hearing Research Institute, University of Michigan Medical Center, Ann Arbor 48109.
authors: P R
authors: PR
authors: Thorne
authors: L
authors: L
authors: Carlisle
authors: G
authors: G
authors: Zajic
authors: J
authors: J
authors: Schacht
authors: R A
authors: RA
authors: Altschuler
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009925:Organ of Corti / Q000033:anatomy & histology*
chemical_list: D000199:Actins