Effect of butylated hydroxyanisole on the metabolism of N-nitrosodi-n-butylamine and N-nitrosobutyl(4-hydroxybutyl)amine by rat hepatic S9 preparations in vitro.
N-Nitrosodi-n-butylamine (NDBA), the NADPH generating system and various concentrations of butylated hydroxyanisole (BHA) added to rat hepatic S9 fractions resulted in a significant drop (30-50%) in N-nitrosobutyl(4-hydroxybutyl)amine (NBHBA) formation and a consequent rise in the amount of substrate recovered unchanged. When NBHBA and NAD+ were incubated with BHA and S9 fractions, the amount of N-nitrosobutyl(3-carboxypropyl)amine (NBCPA) was decreased by 20-40%, and the amount of unmetabolized NBHBA increased.
Pastorelli
R
R
Laboratory of Environmental Pharmacology and Toxicology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Ancidei
A
A
Fanelli
R
R
Airoldi
L
L
D000818:Animals; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002085:Butylhydroxybutylnitrosamine / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats
D009602:Nitrosamines; D002083:Butylated Hydroxyanisole; D002085:Butylhydroxybutylnitrosamine; C006853:butyl(3-carboxypropyl)nitrosamine; C012279:dibutylnitrosamine; D003577:Cytochrome P-450 Enzyme System