title: [Electron-autoradiographic demonstration of intra- and extracellular bactericidal activity of polymorphonuclear leukocytes].
abstract: A technique for the determination of intra- and extra-cellular bactericidal activity of neutrophils has been suggested. A mixture of neutrophils and bacteria was incubated for 30 min and subsequently 3H-uridine was added to the incubated mixture. If phagocytized and extracellularly located bacteria remain alive they incorporate 3H-uridine. Killed bacteria fail to incorporate 3H-uridine. Intra- and extracellular killing capacity of neutrophils is determined by the content of labelled and unlabelled bacteria in radioautographs.
authors: A A
authors: AA
authors: Pal'tsyn
authors: N V
authors: NV
authors: Chervonskaia
authors: A K
authors: AK
authors: Badikova
authors: E K
authors: EK
authors: Uchaneishvili
mesh_terms: D001345:Autoradiography; D001419:Bacteria / Q000378:metabolism; D001770:Blood Bactericidal Activity; D006801:Humans; D008854:Microscopy, Electron; D009504:Neutrophils / Q000502:physiology*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
chemical_list: D014316:Tritium; D014529:Uridine
vernacular_title: Elektronno-radioavtograficheskoe vyiavlenie intra- i ékstratselliuliarnogo bakteritsidnogo deĭstviia polimorfnoiadernykh leĭkotsitov.