Secretion of antileucoprotease from a human lung tumor cell line.
Two human tumor cell lines were analyzed for the production of human antileucoprotease (ALP). One of them, a human squamous lung carcinoma cell line (HS-24) synthesized, as confirmed by Western blot analysis, high amounts of ALP in serum-free medium. The supernatant inhibited elastase, chymotrypsin and trypsin. Northern blot analysis with an 18-mer radiolabelled oligonucleotide, derived from an ALP specific cDNA clone, revealed a specific mRNA of about 700-800 nucleotides in HS-24 tumor cells. In contrast, a secondary human lung tumor cell line (SB-3), derived from the adrenal cortex, did not synthesize ALP when assayed under identical conditions. The supernatant inhibited only trypsin and chymotrypsin.
Appelhans
B
B
Institut für Organische Chemie und Biochemie, Technische Hochschule, Darmstadt, FRG.
Ender
B
B
Sachse
G
G
Nikiforov
T
T
Appelhans
H
H
Ebert
W
W
D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000378:metabolism* / Q000556:secondary; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D003470:Culture Media / Q000032:analysis; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000556:secondary; D009363:Neoplasm Proteins / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D011480:Protease Inhibitors / Q000378:metabolism*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000378:metabolism
D003470:Culture Media; D009363:Neoplasm Proteins; D011480:Protease Inhibitors; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D002918:Chymotrypsin; D010196:Pancreatic Elastase