Fingerstick glycosylated hemoglobin, plasma protein, and albumin.
We have developed techniques that permit the affinity-chromatographic determination of glycosylated hemoglobin, plasma protein, and albumin on fingerstick samples of whole blood. The fingerstick glycohemoglobin technique takes advantage of the high sensitivity of measurement of hemoglobin by absorbance at 414 nm. The glycosylated plasma protein is assayed by a highly sensitive method based on binding of Coomassie blue. An enzyme-linked immunosorbent assay is used to measure albumin in the bound and nonbound fractions of an aminophenylboronic acid chromatographic separation. The fingerstick method for assay of glycosylated plasma albumin gives results that are approximately 40% higher than comparable values obtained on the same patient with a 1-ml plasma sample determined with the bromcresol green technique. There is good correlation of fingerstick glycoalbumins with fingerstick glycohemoglobins and glycosylated plasma protein values. These procedures should be useful for children and for large-scale ambulatory screening for diabetes mellitus.
Rendell
M
M
Creighton University School of Medicine, Omaha, Nebraska.
Brannan
C
C
Nierenberg
J
J
Rasbold
K
K
Hestorff
T
T
D001774:Blood Chemical Analysis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D001800:Blood Specimen Collection / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood*; D006442:Glycated Hemoglobin / Q000032:analysis*; D017127:Glycation End Products, Advanced; D006023:Glycoproteins; D006031:Glycosylation; D006801:Humans; D012709:Serum Albumin / Q000032:analysis*; D000093422:Glycated Serum Proteins; D000093423:Glycated Serum Albumin
D001798:Blood Proteins; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D006023:Glycoproteins; D012709:Serum Albumin; D000093422:Glycated Serum Proteins; D000093423:Glycated Serum Albumin