High-performance liquid chromatography of 2-mercaptopropionylglycine and its metabolite 2-mercaptopropionic acid in plasma and urine after treatment with thiopronine.
2-Mercaptopropionic acid has been identified as a normal metabolite of 2-mercaptopropionylglycine (thiopronine) when this drug was given to humans and dogs. A high-performance liquid chromatographic method was developed to resolve the derivatives of these two thiols and thus enable simultaneous determination of the two compounds in plasma and urine.
Kågedal
B
B
Department of Clinical Chemistry, University Hospital, Linköping, Sweden.
Andersson
T
T
Carlsson
M
M
Denneberg
T
T
Hoppe
A
A
D000603:Amino Acids, Sulfur / Q000302:isolation & purification*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003555:Cystinuria / Q000097:blood / Q000652:urine; D004285:Dogs; D006801:Humans; D013045:Species Specificity; D013438:Sulfhydryl Compounds / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D008625:Tiopronin / Q000302:isolation & purification* / Q000493:pharmacokinetics
D000603:Amino Acids, Sulfur; D013438:Sulfhydryl Compounds; D008625:Tiopronin; C023884:thiolactic acid