title: High-performance liquid chromatography-inductively coupled plasma profiles of cadmium, zinc, sulphur, and other elements in rat liver supernatants after cadmium injection.
abstract: The distribution of cadmium, zinc, sulphur and other elements in the liver supernatants of rats and their variation with time following a single intraperitoneal injection of cadmium chloride were characterized by high-performance liquid chromatography (HPLC) coupled with an inductively coupled plasma. Cadmium that was bound to high-molecular-weight-proteins before the induction of metallothionein was analysed more easily using an Asahipak GS-520 column, whereas the distributions of other elements and of cadmium bound to metallothionein were better characterized on a TSK G3000SW column. Cadmium bound primarily to a distinct high-molecular-weight protein before the induction of metallothionein. After the injection its distribution shifted with time to metallothionein. An atomic emission spectrometer, employing an inductively coupled argon plasma and a vacuum UV monochromator was effectively used as a multi-element specific detector for HPLC.
authors: National Institute for Environmental Studies, Ibaraki, Japan.
authors: K T
authors: KT
authors: Suzuki
authors: H
authors: H
authors: Sunaga
authors: E
authors: E
authors: Kobayashi
authors: N
authors: N
authors: Sugihira
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000032:analysis; D007274:Injections, Intraperitoneal; D008099:Liver / Q000032:analysis*; D008297:Male; D008668:Metallothionein / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000032:analysis; D013455:Sulfur / Q000032:analysis*; D015032:Zinc / Q000032:analysis*
chemical_list: D002104:Cadmium; D013455:Sulfur; D003300:Copper; D008668:Metallothionein; D015032:Zinc