Optimal isolation conditions for rainbow trout (Salmo gairdneri) hepatic ribosomes.
1. Isolation conditions for rainbow trout (Salmo gairdneri) liver ribosomes were optimized. 2. Optimal initial buffer (Buffer I) concentrations were 250 mM sucrose, 50 mM Tris (pH 7.6, 25 degrees C), 75 mM KCl, and 5 mM MgSO4.7H2O. 3. Optimal concentrations for post-105 supernatant buffer (Buffer III) were 25 mM Tris (pH 7.6, 25 degrees C), 75 mM KCl, and 8 mM MgSO4.7H2O.
Orvos
D R
DR
Department of Biology, James Madison University, Harrisonburg, VA 22807.
Sellers
C M
CM
Garrison
N E
NE
D000818:Animals; D002021:Buffers; D002458:Cell Fractionation / Q000379:methods; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D008278:Magnesium Sulfate; D011189:Potassium Chloride; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure*; D014325:Tromethamine; D014337:Trout; D014461:Ultracentrifugation
D002021:Buffers; D014325:Tromethamine; D011189:Potassium Chloride; D008278:Magnesium Sulfate