[Chemical reactions in double-stranded nucleic acids. The nature of the bond formed during chemical ligation using a cis-diol group].
Chemical and enzymatic ligation between the 5'-terminal phosphate of one oligonucleotide and the 3'-terminal 2',3'-cis-diol group of the other oligonucleotide on a complementary template was studied. Carbodiimide, imidazolide and N-hydroxybenzotriazole ester methods were used for chemical activation of the phosphate group, and T4 DNA ligase for enzymatic ligation. All the chemical activation methods produced 3',5'- and 2',5'-phosphodiester bonds (40-45 and 55-60%, resp.), whereas enzymatic ligation gave the product only with 3',5'-phosphodiester bond.
Kuznetsova
S A
SA
Ivanovskaia
M G
MG
Shabarova
Z A
ZA
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000138:chemical synthesis*; D004274:DNA, Recombinant; D006868:Hydrolysis; D009841:Oligonucleotides / Q000138:chemical synthesis
D004274:DNA, Recombinant; D009841:Oligonucleotides; D004247:DNA
Khimicheskie reaktsii v dvukhspiral'nykh nukleinovykh kislotakh. Priroda sviazi, obrazuiushcheĭsia pri khimicheskom ligirovanii s uchastiem tsis-diol'noĭ gruppirovki.