[Addressed modification of the promoter region of DNA in a complex with RNA polymerase by alkylating oligonucleotide derivatives].
Addressed chemical modification of double-stranded DNA unwound with the specific protein has been demonstrated. 4(N-2-chloroethyl-N-methylamino)benzyl-5'-phosphoamides of oligonucleotides d(CG)rC, d(ATCG)rC, d(AATCG)rC, which can serve as primers in the RNA polymerase-catalyzed transcription, alkylated A2 promoter region of pSK-A2 plasmid in its "open" complex with E. coli RNA polymerase.
Butorin
A S
AS
Vlasov
V V
VV
Grachev
M A
MA
Zaĭchikov
E F
EF
Konovets
D A
DA
D000477:Alkylating Agents; D004247:DNA / Q000032:analysis*; D012321:DNA-Directed RNA Polymerases; D004587:Electrophoresis, Agar Gel; D009841:Oligonucleotides; D010957:Plasmids; D011401:Promoter Regions, Genetic
D000477:Alkylating Agents; D009841:Oligonucleotides; D004247:DNA; D012321:DNA-Directed RNA Polymerases
Adresovannaia modifikatsiia promotornogo uchastka DNK v komplekse c RNK-polimerazoĭ alkiliruiushchimi proizvodnymi oligonukleotidov.