title: Immunochemical and immunocytochemical studies of the crustacean vitellogenesis-inhibiting hormone (VIH).
abstract: Immunochemical investigations, using dot immunobinding assay (DIA) and enzyme-linked immunosorbent assay (ELISA), and immunocytochemical studies reveal the following new information about crustacean vitellogenesis-inhibiting hormone (VIH): (1) The structure of VIH is sufficiently different from that of the other sinus gland neuropeptides to allow a selective recognition of VIH by polyclonal antibodies. (2) From immunochemical criteria, VIH does not seem strictly species specific. The antisera raised against VIH of Homarus americanus cross-react with sinus gland extracts of Palaemonetes varians, Palaemon serratus, Macrobrachium rosenbergii, Carcinus maenas, and Porcellio dilatatus. (3) In the sinus gland of H. americanus, VIH immunoreactivity is localized mainly in electron-dense granules of medium size (110-185 nm in diameter) while, in P. dilatatus, the labeling is mostly on the largest granules (200-270 nm in diameter).
authors: Laboratoire de Physiologie de la Reproduction (Groupe Crustacés), U.A. 555, Université Pierre et Marie Curie.
authors: J J
authors: JJ
authors: Meusy
authors: G
authors: G
authors: Martin
authors: D
authors: D
authors: Soyez
authors: J E
authors: JE
authors: van Deijnen
authors: J M
authors: JM
authors: Gallo
mesh_terms: D000818:Animals; D003445:Crustacea / Q000032:analysis*; D004702:Endocrine Glands / Q000032:analysis / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D007447:Invertebrate Hormones / Q000032:analysis*; D008121:Nephropidae / Q000032:analysis*; D013045:Species Specificity; D014818:Vitellogenesis
chemical_list: D007447:Invertebrate Hormones