Immunological relation between serum antibodies against pneumolysin and against streptolysin O.
The immunological relation between serum antibodies to pneumolysin and to streptolysin O was studied in patients with pneumococcal pneumonia (n = 40), patients with infections due to beta-haemolytic streptococci (n = 35), healthy human controls (n = 60) and in rabbits immunized with pneumolysin. There was no correlation between anti-pneumolysin and anti-streptolysin O titers (r = -0.279). The distribution of anti-pneumolysin titers in patients with high anti-streptolysin O titers did not differ from healthy controls. However, there was a tendency to increased or rising anti-streptolysin O titers in patients with pneumococcal infection. Antibodies obtained during pneumococcal infection might thus give false-positive reactions in the streptolysin O neutralization test. Serum antibodies to streptolysin O do not cross-react with pneumolysin in an ELISA. The pneumolysin ELISA for detection of pneumococcal disease will therefore not be disturbed by false-positive reactions due to antibodies directed against beta-haemolytic streptococci.
Kanclerski
K
K
National Bacteriological Laboratory, Stockholm, Sweden.
Granström
M
M
Möllby
R
R
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D000989:Antistreptolysin / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins; D003429:Cross Reactions; D003603:Cytotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009500:Neutralization Tests; D011018:Pneumonia, Pneumococcal / Q000276:immunology*; D011817:Rabbits; D013290:Streptococcal Infections / Q000276:immunology*; D013301:Streptolysins / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D003603:Cytotoxins; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae; C025964:streptolysin O; D000989:Antistreptolysin