title: Identification of drugs in human serum by high-performance liquid chromatography with photodiode array detection and a search algorithm for ultraviolet spectra.
abstract: A system is described that identifies a number of unknown drugs (benzodiazepines, antidepressants and neuroleptics) in blood specimens for clinical-toxicological purposes. Reversed ion-pair high-performance liquid chromatography with a photodiode array detector saves the ultraviolet spectrum of every chromatographically significant peak. Post-run data-processing, provided by a microcomputer, retrieves candidate substances from a library of ultraviolet spectra. Selected standard ultraviolet spectra are compared with the unknown by five different similarity tests. The discriminatory efficiency of these algorithms has been determined. Multicomponent analysis, a built-in program of the spectrophotometer, provided the most reliable results.
authors: Central Laboratory of Clinical Chemistry, University Hospital, Zurich, Switzerland.
authors: E I
authors: EI
authors: Minder
authors: R
authors: R
authors: Schaubhut
authors: C E
authors: CE
authors: Minder
authors: D J
authors: DJ
authors: Vonderschmitt
mesh_terms: D000465:Algorithms; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006801:Humans; D007256:Information Systems; D004364:Pharmaceutical Preparations / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation
chemical_list: D004364:Pharmaceutical Preparations