Pharmacokinetics of T-2 tetraol, a urinary metabolite of the trichothecene mycotoxin, T-2 toxin, in dog.
1. The urinary metabolites of T-2 toxin were identified and analysed quantitatively after i.v. administration to dogs. 2. A new routine assay for T-2 tetraol was developed and a pharmacokinetic study was carried out on this final hydrolytic metabolite of T-2 toxin. T-2 tetraol was excreted in urine for 2-3 days. Its 'sigma minus' plot demonstrated a significantly longer apparent half-life than its precursors (T-2 toxin and HT-2 toxin). This fact was explained by extraplasma binding causing prolongation of the metabolism and excretion of T-2 toxin metabolites. 3. The urinary metabolites of T-2 toxin were: HT-2 toxin, T-2 triol and T-2 tetraol. The metabolites were excreted in free and conjugated forms. In two dogs T-2 toxin was found in the urine in an amount which accounts for 3.2 and 16% of the administered dose respectively. The cumulative amount of the identified metabolites and toxins formed in the urine ranged from 9.7 to 17.3% in four dogs and 44.7% in one dog.
Sintov
A
A
Department of Pharmacy, School of Pharmacy, Hebrew University, Jerusalem, Israel.
Bialer
M
M
Yagen
B
B
D000818:Animals; D002849:Chromatography, Gas; D004285:Dogs; D005260:Female; D006207:Half-Life; D006868:Hydrolysis; D008297:Male; D012717:Sesquiterpenes / Q000493:pharmacokinetics*; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000493:pharmacokinetics* / Q000652:urine
D012717:Sesquiterpenes; C046700:T-2 tetraol; D013605:T-2 Toxin