title: Purification of adenovirus hexon protein by high-performance liquid chromatography.
abstract: Adenovirus (Type 2)-infected HeLa cells were sonicated and treated with 1,1,2-trichlorotrifluoroethane. The water-soluble extract was ultracentrifuged and the supernatant, containing the dissociated proteins, was subjected to anion-exchange high-performance liquid chromatography on a Mono Q column in 50 mM bis-Tris-HCl (pH 6.5). Elution with a linear salt gradient resulted in a major peak, containing the hexon protein.
authors: Department of Virology, University of Turku, Finland.
authors: M
authors: M
authors: Waris
authors: P
authors: P
authors: Halonen
mesh_terms: D000256:Adenoviridae / Q000032:analysis*; D002213:Capsid / Q000032:analysis*; D036022:Capsid Proteins; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus