title: Short-chain fatty acids and succinate in feces of healthy human volunteers and their correlation with anaerobe cultural counts.
abstract: Short-chain fatty acids (SCFA) and succinate in fresh feces from 10 healthy human volunteers were detected with gas-liquid chromatography and were correlated with their fecal cultural counts. The SCFA content of feces did not change significantly after freezing, but the cultural counts of total anaerobes and bifidobacteria showed significant changes (0.001 less than P less than 0.01). A significant negative correlation (r = -0.78; 0.01 less than P less than 0.02) was found between the bifidobacteria cultural counts and the succinic acid concentration in fresh feces, which disappeared after freezing of the samples. Because of the significant differences in cultural counts and the shift in significant correlations after freezing, it is important to use fresh fecal samples for these correlation studies. Lactic acid and i-caproic acid were not found in the fecal samples. The results show that SCFA and organic acid concentrations cannot replace (an)aerobe cultural counts in fecal flora studies of healthy human volunteers because of the low rate of significant correlations between these two.
authors: Laboratory for Medical Microbiology, State University of Groningen, The Netherlands.
authors: G J
authors: GJ
authors: Meijer-Severs
authors: E
authors: E
authors: van Santen
mesh_terms: D000328:Adult; D001420:Bacteria, Aerobic / Q000032:analysis*; D001421:Bacteria, Anaerobic / Q000032:analysis*; D002849:Chromatography, Gas; D005232:Fatty Acids, Volatile / Q000032:analysis*; D005243:Feces / Q000032:analysis* / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D013048:Specimen Handling; D013386:Succinates / Q000032:analysis*
chemical_list: D005232:Fatty Acids, Volatile; D013386:Succinates