Histochemistry of MAO-A and MAO-B in the locus coeruleus of the Mongolian gerbil.
A coupled peroxidation technique for localization of monoamine oxidase (MAO-A and MAO-B), applied to post-mortem fixed tissue of the locus coeruleus of the Mongolian gerbil is demonstrated. Tyramine hydrochloride, beta-phenylethylamine and 5-hydroxytryptamine creatinine sulphate were used as substrates, 1-deprenyl and clorgyline served as specific inhibitors. All three substrates stained the neurons of locus coeruleus in the absence of inhibitor. In the presence of 1-deprenyl, tyramine hydrochloride and 5-hydroxytryptamine creatinine sulphate were metabolized, whereas in the presence of clorgyline no reaction with either substrate could be observed. Immunocytochemical staining of tyrosine hydroxylase (TH) was employed as comparison.
Konradi
C
C
Department of Psychiatry, University of Würzburg, Federal Republic of Germany.
Jellinger
K
K
Riederer
P
P
Nagatsu
T
T
D000818:Animals; D005849:Gerbillinae; D006651:Histocytochemistry; D008125:Locus Coeruleus / Q000032:analysis / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000032:analysis*
D008995:Monoamine Oxidase