Iron-mediated oxidative stress in erythrocytes.
Erythrocytes subjected extracellularly to iron-mediated oxidant stress undergo haemoglobin oxidation and membrane damage, which can be modulated by maintaining the energy requirements of the cells. The results presented here suggest that a balance exists between the oxidation state of the haemoglobin and the oxidative deterioration of the membrane lipids, which is dependent on the metabolic state of the erythrocytes. These findings have important implications for thalassaemic erythrocytes that may be exposed to excess plasma iron levels, in which excessive membrane-bound iron in the form of haemichromes is a characteristic feature and in which cellular ATP levels are lowered.
Rice-Evans
C
C
Department of Biochemistry, Royal Free Hospital School of Medicine, London, U.K.
Baysal
E
E
D000255:Adenosine Triphosphate / Q000097:blood; D001205:Ascorbic Acid / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005978:Glutathione / Q000097:blood; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D010084:Oxidation-Reduction
D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D007501:Iron; D005978:Glutathione; D003676:Deferoxamine; D001205:Ascorbic Acid