Fecal short-chain fatty acid (SCFA) analysis by capillary gas-liquid chromatography.
A new method is described for short-chain fatty acid (SCFA) analysis in stool samples. After sample purification by acid vacuum transfer and concentration by alkaline freeze-drying, SCFAs were measured by gas-liquid chromatography using a capillary column. Peak resolution and reproducibility were superior to SCFA analysis on a conventional glass column. With this new technique SCFA were quantitated in stool samples of six healthy volunteers on a basal diet with and without a fermentable dietary fiber preparation; fiber had no effect on fecal SCFA concentration. In two patients with a short-bowel syndrome, SCFA could be demonstrated in stool samples, indicating active fermentation.
Scheppach
W M
WM
Department of Medicine, Wuerzburg University, Wuerzburg, FRG.
Fabian
C E
CE
Kasper
H W
HW
D000085:Acetates / Q000032:analysis; D000328:Adult; D002087:Butyrates / Q000032:analysis; D020148:Butyric Acid; D002849:Chromatography, Gas / Q000379:methods; D003106:Colon / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis*; D005243:Feces / Q000032:analysis*; D005260:Female; D005285:Fermentation; D006801:Humans; D008297:Male; D011422:Propionates / Q000032:analysis; D012778:Short Bowel Syndrome / Q000378:metabolism
D000085:Acetates; D002087:Butyrates; D004040:Dietary Carbohydrates; D005232:Fatty Acids, Volatile; D011422:Propionates; D020148:Butyric Acid