The kinetics of endogenous erythroid haemopoietic spleen colonies in mice between 4 and 10 days after irradiation with doses of 5, 7, or 9 Gy.
The kinetics of post-radiation haemopoietic tissue recovery was studied by evaluating quantitatively the results from histologic studies of the kinetics of endogenous erythroid spleen colony counts in subserial sections of spleens from mice irradiated with 5, 7, or 9 Gy of 60Co gamma-rays at intervals of 4, 6, 8, and 10 days following irradiation. Emphasis was put on individual types of colony-forming cells and on time intervals at which these cells enter into action. Large numbers of rapidly maturing microscopic erythroid colonies appeared between 8 and 10 days after irradiation. From this finding we conclude that in addition to colony-forming cells giving rise to macroscopic colonies, large numbers of already differentiated progenitor cells--the products of the first regeneration wave with a peak approximately 6 days following irradiation--participate in the second wave of regeneration which is typically manifested from day 10 after irradiation.
Hofer
M
M
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Tkadlecek
L
L
Viklická
S
S
Karpfel
Z
Z
D000818:Animals; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D004912:Erythrocytes / Q000166:cytology; D004920:Erythropoiesis / Q000528:radiation effects*; D005720:Gamma Rays; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000528:radiation effects*; D013997:Time Factors