An enzyme-linked lectin-binding assay on cells (CELLBA) for the comparison of lectin receptor expression on cell surfaces.
Lectins can be used to specifically detect some cell surface glycans. Their expression on different cells or on cells of a given lineage throughout differentiation or following treatment with drugs can be compared using lectins labelled with radioactive, fluorescent or enzymatic probes. We describe a new method which, by analogy with CELISA (ELISA on cells), is called CELLBA (or ELLBA on cells) for cellular, enzyme-linked lectin-binding assay. It permits the comparison of the expression of specific glycans in a large number of different cell samples. As an example, it was able to detect alterations of cell surface glycan expression caused by inhibitors of N-linked oligosaccharide trimming.
Gavériaux
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Preclinical Research Department, Sandoz Ltd., Basel, Switzerland.
Loor
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D000371:Agglutination; D000818:Animals; D001710:Biotin; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006001:Glycoconjugates / Q000032:analysis*; D037102:Lectins / Q000378:metabolism*; D008361:Mannosidases / Q000037:antagonists & inhibitors; D051379:Mice; D009005:Monosaccharides / Q000378:metabolism; D010969:Plastics; D051381:Rats; D011975:Receptors, Mitogen / Q000032:analysis*
D006001:Glycoconjugates; D037102:Lectins; D009005:Monosaccharides; D010969:Plastics; D011975:Receptors, Mitogen; D001710:Biotin; D008361:Mannosidases