title: Column purification of adenovirus cores.
abstract: A simple and efficient technique for purifying adenoviral chromatin (nucleoprotein cores) with Sephacryl S-1000 is described. This method is significantly faster than previous methods and gives a higher degree of purity with an increased recovery of the nucleoprotein. In addition, the structural integrity of the cores is maintained.
authors: Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
authors: J F
authors: JF
authors: Cupo
authors: L J
authors: LJ
authors: Rezanka
authors: J A
authors: JA
authors: Harpst
mesh_terms: D000180:Acrylic Resins; D000260:Adenoviruses, Human / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D014758:Viral Core Proteins / Q000302:isolation & purification*
chemical_list: D000180:Acrylic Resins; C037239:Sephacryl Superfine; D014758:Viral Core Proteins