title: Vasopressor peptides and depolarization stimulated Ca2+-entry into cultured vascular smooth muscle.
abstract: 45Ca-uptake was measured in monolayers of cultured rat aortic smooth muscle cells. Sufficient extracellular 45Ca could be removed by a 90 second cold La3+ was to reveal stimulation of 45Ca-uptake by high K+-depolarization and the vasopressor peptides angiotensin II and vasopressin. The high K+-stimulated 45Ca-influx was blocked by a dihydropyridine-type Ca2+-antagonist while that stimulated by angiotensin II or vasopressin was not. The 45Ca-influx stimulated by high K+-depolarization was additive to that stimulated by angiotensin II. Vasopressin and angiotensin II stimulated 45Ca-fluxes were not additive. It is concluded that vasopressor peptides stimulate Ca2+-entry through receptor operated Ca2+-channels which are distinct from voltage gated Ca2+-channels.
authors: Department of Research, University Hospital, Basel, Switzerland.
authors: A
authors: A
authors: Zschauer
authors: T
authors: T
authors: Scott-Burden
authors: F R
authors: FR
authors: Bühler
authors: C
authors: C
authors: van Breemen
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004553:Electric Conductivity; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D014667:Vasopressins / Q000494:pharmacology*
chemical_list: D014667:Vasopressins; D000804:Angiotensin II; D011188:Potassium; D002118:Calcium