title: Bromotrichloromethane-induced damage to bronchiolar Clara cells.
abstract: Administration of bromotrichloromethane (BrCCl3) to rats results in selective damage to bronchiolar non-ciliated Clara cells; ciliated bronchiolar cells and pneumocytes were unaffected. The cellular alterations begin very early (10 min) after poisoning. Lipid peroxidation, as measured by the malonic dialdehyde (MDA) content in the lung, is greatly increased 10 min after BrCCl3 administration. A histochemical technique to detect, in vitro, lipid peroxidation in frozen sections was used to demonstrate whether sufficient activation of BrCCl3 occurs in lung tissue. The positivity for the histochemical reaction was observed in bronchiolar epithelium in which cytochrome P450-dependent monooxygenase activity is predominantly located. The data obtained strongly support that BrCCl3 is highly metabolized in bronchiolar Clara cells.
authors: Institute of General Pathology, University of Siena, Italy.
authors: G
authors: G
authors: Lungarella
authors: A
authors: A
authors: Benedetti
authors: C
authors: C
authors: Gardi
authors: M M
authors: MM
authors: de Santi
authors: M
authors: M
authors: Comporti
mesh_terms: D000818:Animals; D001975:Bromotrichloromethane / Q000378:metabolism / Q000633:toxicity*; D001980:Bronchi / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D002251:Carbon Tetrachloride / Q000633:toxicity; D002725:Chloroform / Q000031:analogs & derivatives*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008054:Lipid Peroxides; D002725:Chloroform; D002251:Carbon Tetrachloride; D001975:Bromotrichloromethane