An enzyme immunoassay for the quantitation of dihydropteridine reductase.
A competitive, enzyme-linked immunosorbent assay for the quantitative determination of dihydropteridine reductase (DHPR) is described. This highly sensitive method can determine the content of DHPR protein in tissue preparations independently of the enzymatic activity of the protein molecule. The method involves initial incubation of samples containing soluble enzyme in microtiter plates coated with purified goat antibodies to rat DHPR and further incubation with DHPR conjugated to alkaline phosphatase. The assay is used to study the ontogeny of DHPR in rat liver.
Kwan
S W
SW
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
Shen
R S
RS
Abell
C W
CW
D000367:Age Factors; D000818:Animals; D000918:Antibody Specificity; D004093:Dihydropteridine Reductase / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D008099:Liver / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D004093:Dihydropteridine Reductase; D009247:NADH, NADPH Oxidoreductases