Exhalation of N-nitrosoethylvinylamine after application of N-nitrosodiethylamine to Sprague-Dawley rats.
Our method of endotracheal intubation makes it possible to collect exhaled air directly from the respiratory tract, thus eliminating the possibility of artefact formation and decomposition of metabolites. N-Nitrosodiethylamine (NDEA) has been postulated as a precursor of N-nitrosoethylvinylamine (NEVA), however, NEVA has not been detected as a metabolite of NDEA. Following endotracheal intubation and intravenous application of 550 micrograms NDEA to Sprague-Dawley rats, appreciable amounts of NEVA and unaltered NDEA were found in exhaled air. Further confirmation that NEVA is a metabolite of NDEA was obtained when, after eliminating oxidative decomposition of the nitrosamine with disulfiram in an enzyme inhibition assay, pretreated rats exhaled only traces of NEVA. Such findings could be informative with respect to the organotropism of nitrosamine carcinogenesis.
Klein
R G
RG
Institute for Toxicology and Chemotherapy, German Cancer Research Center, Heidelberg, Federal Republic of Germany.
Schmezer
B
B
Schmezer
P
P
Spiegelhalder
B
B
D000818:Animals; D001944:Breath Tests; D004052:Diethylnitrosamine / Q000378:metabolism*; D005260:Female; D009602:Nitrosamines / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D009602:Nitrosamines; C032754:vinylethylnitrosamine; D004052:Diethylnitrosamine