Determination of hippurate hydrolysis by gas-liquid chromatography.
A rapid gas-liquid chromatographic procedure was developed to determine hippurate hydrolysis by microorganisms. Bacterial cells were inoculated into 0.4 ml of 1% sodium hippurate and incubated for 2 h at 37 degrees C. Cells were removed by centrifugation, and the benzoate released by enzyme activity was converted to methyl benzoate and analyzed by gas-liquid chromatography. This procedure is sensitive, and its specificity provides a high degree of reliability for organisms with weak hippuricase activity.
Wallace
P L
PL
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Patton
C M
CM
Moss
C W
CW
D001565:Benzoates / Q000032:analysis*; D002167:Campylobacter / Q000378:metabolism*; D002168:Campylobacter fetus / Q000378:metabolism*; D002849:Chromatography, Gas; D006626:Hippurates / Q000378:metabolism*; D006868:Hydrolysis; D007875:Legionella / Q000378:metabolism*; D011237:Predictive Value of Tests
D001565:Benzoates; D006626:Hippurates