title: Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions.
abstract: Bovine anterior-pituitary microsomal fractions exhibit high-affinity, saturable and reversible binding of inositol 1,4,5-[32P]trisphosphate; 50% of the labelled ligand is displaced by 3.5 nM-inositol 1,4,5-trisphosphate. 0.5 microM-inositol 1,4-bisphosphate and 10 microM-ATP. Inositol 1,4,5-trisphosphate induces the release of Ca2+ from the microsomal vesicles (half-maximal effect at 290 nM), and its action is potentiated by inositol tetrakisphosphate (half-maximal effect at 4 microM).
authors: Department of Physilogy, Semmelweis University Medical School, Budapest, Hungary.
authors: A
authors: A
authors: Spät
authors: G L
authors: GL
authors: Lukács
authors: I
authors: I
authors: Eberhardt
authors: L
authors: L
authors: Kiesel
authors: B
authors: B
authors: Runnebaum
mesh_terms: D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D007295:Inositol Phosphates / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism*
chemical_list: D007295:Inositol Phosphates; D013403:Sugar Phosphates; D002118:Calcium