title: A new tetracycline antibiotic from a Dactylosporangium species. Fermentation, isolation and structure elucidation.
abstract: An actinomycete identified as a Dactylosporangium sp. produces a new tetracycline, 4a-hydroxy-8-methoxychlortetracycline (Sch 34164). The addition of magnesium ions to complex fermentation media increased the antibiotic titers. Sch 34164 was isolated by solvent extraction and Sephadex G-25 column chromatography. The novel structure was proposed based on spectroscopic analysis. The shift of C-4a (35 to 77 ppm) and C-8 (140 to 163 ppm) in the 13C NMR as compared to chlortetracycline was indicative of the novel hydroxyl and methoxy substituents, respectively.
authors: Department of Microbial Products, Schering Corporation, Bloomfield, NJ 07003.
authors: M
authors: M
authors: Patel
authors: V P
authors: VP
authors: Gullo
authors: V R
authors: VR
authors: Hegde
authors: A C
authors: AC
authors: Horan
authors: J A
authors: JA
authors: Marquez
authors: R
authors: R
authors: Vaughan
authors: M S
authors: MS
authors: Puar
authors: G H
authors: GH
authors: Miller
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002751:Chlortetracycline / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy
chemical_list: C054353:dactylocyclinone; D002751:Chlortetracycline