Foam counter-current chromatography with the cross-axis synchronous flow-through coil planet centrifuge.
A novel coil planet centrifuge system performs efficient foam separation in a long multilayer coil. Potential capability of the method was successfully demonstrated in separations of two sets of test samples using sodium dodecyl sulfate as a foaming agent. Rhodamine B with high foam affinity was quickly separated from Evans blue lacking foam affinity. A high concentration of salts such as disodium hydrogenphosphate in the surfactant solution effectively lowered the foam affinity of basic dyes, yielding complete peak resolution of rhodamine B and methylene blue according to a subtle difference in foam affinity.
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National Heart, Lung, and Blood Institute, Laboratory of Technical Development, Bethesda, MD 20892.
D002498:Centrifugation / Q000295:instrumentation*; D002845:Chromatography / Q000295:instrumentation*; D004396:Coloring Agents; D007202:Indicators and Reagents; D008751:Methylene Blue / Q000032:analysis; D012235:Rhodamines / Q000032:analysis
D004396:Coloring Agents; D007202:Indicators and Reagents; D012235:Rhodamines; C029773:rhodamine B; D008751:Methylene Blue