A simple and sensitive determination of histamine and N tau-methylhistamine in biological fluids by high-performance liquid chromatography with electrochemical detection.
The determination of picomolar levels of histamine and its major metabolite, N tau-methylhistamine, in biological fluids was achieved using reversed-phase liquid chromatography coupled with electrochemical detection. A simple sample purification procedure for blood and urine samples was carried out prior to analysis using an Amberlite CG-50 cation-exchange resin, which afforded an excellent recovery of both compounds.
Houdi
A A
AA
College of Pharmacy, Division of Medicinal Chemistry and Pharmacognosy, University of Kentucky, Lexington 40536-0053.
Crooks
P A
PA
Van Loon
G R
GR
Schubert
C A
CA
D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis*; D008297:Male; D008761:Methylhistamines / Q000032:analysis*
D008761:Methylhistamines; C031205:3-methylhistamine; D006632:Histamine