Benzoyl peroxide activation of protein kinase C activity in epidermal cell membranes.
We have investigated the effects of various tumor promoting agents on protein kinase C activity in adult female SENCAR mice. Topical application of benzoyl peroxide increased the calcium-independent activity of protein kinase C in the particulate fraction of basal epidermal cells while decreasing protein kinase C activity in the soluble fraction of basal epidermal cells isolated from the mice. Benzoyl peroxide apparently altered protein kinase C indirectly, as it had no direct effect on the activity of protein kinase C partially purified from bovine brain. Topical application of 12-O-tetradecanoyl phorbol-13-acetate to mouse skin resulted in increased protein kinase C activity associated with the particulate fraction of the epidermal cells with no change in the calcium-dependence of the enzyme. Since the proliferative agent ethylphenylpropiolate had no effect on protein kinase C in epidermis from SENCAR mice, the ability of benzoyl peroxide to influence protein kinase C activity in mouse skin may reflect its tumor promoting capabilities rather than its ability to induce hyperplasia.
Donnelly
T E
TE
Department of Pharmacology, University of Nebraska Medical Center, Omaha 68105.
Pelling
J C
JC
Anderson
C L
CL
Dalbey
D
D
D000480:Alkynes / Q000494:pharmacology; D000818:Animals; D001585:Benzoyl Peroxide / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D004789:Enzyme Activation; D000078404:Epidermal Cells; D004817:Epidermis / Q000201:enzymology*; D005260:Female; D051379:Mice; D010545:Peroxides / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000480:Alkynes; D010545:Peroxides; C015202:ethylphenylpropiolate; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D001585:Benzoyl Peroxide