Preparation and characterization of radioactive castanospermine.
A procedure for the preparation of tritiated castanospermine is described. The tritiated alkaloid was shown to be chromatographically identical to the native material and exhibited the same inhibitory properties. Radiolabeled castanospermine tightly bound to purified intestinal sucrase. Following gel chromatography, each mole of enzyme was shown to have bound 1 mol of the radioactive alkaloid. Cultured MDCK cells were also shown to take up the labeled castanospermine. This compound should be a useful tool in the investigation of enzymes that are responsible for the processing of glycoprotein oligosaccharides.
Keenan
R W
RW
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284.
Pan
Y T
YT
Elbein
A D
AD
D000470:Alkaloids / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D007887:Fabaceae / Q000032:analysis; D005260:Female; D007212:Indolizines; D007422:Intestines / Q000201:enzymology; D010946:Plants, Medicinal; D051381:Rats; D013393:Sucrase / Q000037:antagonists & inhibitors; D014018:Tissue Distribution; D014316:Tritium; D054836:Indolizidines
D000470:Alkaloids; D007212:Indolizines; D013393:Sucrase; D014316:Tritium; C037806:castanospermine; D054836:Indolizidines