Vasopressin gene transcripts in the bovine corpus luteum are defective.
cDNA clones corresponding to vasopressin gene transcripts were isolated from a lambda gt11 library made using mRNA extracted from a bovine corpus luteum of the early non-pregnant cycle. None of the characterized clones included the vasopressin-encoding exon A sequence, instead two of these clones included sequence from the first intron. Together these data and controls indicate that vasopressin gene transcription in this tissue does not yield translatable mRNA and that positive RNA-DNA hybridization signals are not necessarily evidence for local biosynthesis of the neuropeptide.
Morley
S D
SD
Institut für Zellbiochemie und klinische Neurobiologie, Universität Hamburg, F.R.G.
Ivell
R
R
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003338:Corpus Luteum / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D005260:Female; D066298:In Vitro Techniques; D007438:Introns; D008969:Molecular Sequence Data; D014158:Transcription, Genetic; D014667:Vasopressins / Q000235:genetics* / Q000378:metabolism
D014667:Vasopressins; D004247:DNA