Antibodies to Coprococcus comes in sera of patients with Crohn's disease. Isolation and purification of the agglutinating antigen tested with an ELISA technique.
Previous studies showed that agglutinating antibodies to Coprococcus comes, an anaerobic Gram-positive coccoid rod isolated from the faecal flora of patients with Crohn's disease, are more frequently found in sera of Crohn patients than in ulcerative colitis patients and healthy subjects. Isolation of the antigen may be useful in developing a more sensitive and specific diagnostic test. The present study describes first a method to improve the presentation of the relevant agglutinating antigen by the bacterium and second, the purification by column chromatography of a relatively crude antigen extract of C. comes described previously by Hazenberg et al. (1). Comparative results with the agglutination reactions and ELISA technique of extensive series of patients with Crohn's disease and healthy subjects have shown that the agglutinating antigen of C. comes has been isolated. Although the present ELISA technique cannot replace the simple and reliable agglutination reaction for screening purposes, the purified antigen will allow further immunological studies and it is to be hoped that a deeper insight into pathogenesis of the disease will be gained.
Hazenberg
M P
MP
Department of Medical Microbiology, Erasmus University, Rotterdam, The Netherlands.
van de Merwe
J P
JP
Peña
A S
AS
Pennock-Schröder
A M
AM
van Lieshout
L M
LM
D000372:Agglutination Tests / Q000379:methods; D000373:Agglutinins / Q000032:analysis / Q000302:isolation & purification*; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D001421:Bacteria, Anaerobic / Q000276:immunology*; D002850:Chromatography, Gel / Q000379:methods; D003424:Crohn Disease / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans
D000373:Agglutinins; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial