Determination of aspirin by pre-column transacetylation reaction of 3-aminophenol and reversed-phase high-performance liquid chromatography: simultaneous determination of aspirin, acetaminophen, and caffeine.
The accuracy of the measurement of aspirin by high-performance liquid chromatography (HPLC) is reduced by its hydrolysis into salicylic and acetic acids during sample preparation. The ready and quantitative transacetylation of 3-aminophenol by aspirin, giving 3-hydroxyacetanilide (which is stable), has been utilized as a pre-column reaction for the determination of aspirin either alone or in the presence of acetaminophen and caffeine by reversed-phase HPLC.
Verma
K K
KK
Department of Chemistry, Rani Durgavati University, Jabalpur, India.
Sanghi
S K
SK
Jain
A
A
Gupta
D
D
D000082:Acetaminophen / Q000008:administration & dosage / Q000032:analysis*; D000107:Acetylation; D000627:Aminophenols; D001241:Aspirin / Q000008:administration & dosage / Q000032:analysis*; D002110:Caffeine / Q000008:administration & dosage / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations
D000627:Aminophenols; D004338:Drug Combinations; D000082:Acetaminophen; D002110:Caffeine; D001241:Aspirin