title: Effects of free fatty acids, lysophosphatides and phospholipase treatment on lipid peroxidation of myocardial homogenates and membrane fractions.
abstract: The effects of various free fatty acids, lysophosphatides and phospholipase treatments on the enzymatic and the non-enzymatic lipid peroxidation capacities in the heart homogenates and subcellular fractions were studied. The results showed a dose related inhibition of both the enzymatic and non-enzymatic lipid peroxidation with free fatty acids. A significant inhibition occurred as early as at the concentration of 25-50 microM of several fatty acids both in homogenates and in organelle fractions. In general, the inhibition was greatest with cis-unsaturated, long-chain fatty acids. The inhibition was also induced by the pretreatment of the homogenates with phospholipase A2 but not with phospholipase C. The lysophosphatidyl cholines (16:0 and 18:1) had a stimulatory effect on the enzymatic lipid peroxidation capacity at the physiological concentrations. The results show that the stimulatory/inhibitory effect of various lipid amphiphiles on lipid peroxidation is strongly structure linked and the mitochondrial fraction is the most susceptible to the injury induced by lipid amphiphiles.
authors: Department of Cell Biology, University of Jyväskylä, Finland.
authors: M
authors: M
authors: Kihlström
authors: V
authors: V
authors: Marjomäki
authors: A
authors: A
authors: Salminen
mesh_terms: D000818:Animals; D003327:Coronary Disease / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008055:Lipids / Q000494:pharmacology*; D008246:Lysophospholipids / Q000494:pharmacology; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D010740:Phospholipases / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005230:Fatty Acids, Nonesterified; D008054:Lipid Peroxides; D008055:Lipids; D008246:Lysophospholipids; D008563:Membrane Lipids; D010740:Phospholipases