A protocol and guide for the in vitro rat hepatocyte DNA-repair assay.
The in vitro rat-hepatocyte DNA-repair assay is a valuable tool in assessing the genotoxic activity of chemical agents. An advantage of the assay is that the target cells themselves are metabolically competent, so that the patterns of metabolic activation and detoxification closely reflect those in the whole animal. This article provides a typical procedure and guidelines for conducting the rat in vitro hepatocyte DNA-repair assay.
Butterworth
B E
BE
Chemical Industry Institute of Toxicology, Research Triangle Park, NC 27709.
Ashby
J
J
Bermudez
E
E
Casciano
D
D
Mirsalis
J
J
Probst
G
G
Williams
G
G
D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects*; D009152:Mutagenicity Tests / Q000592:standards*; D009153:Mutagens / Q000032:analysis*; D051381:Rats
D009153:Mutagens