Biochemical characterization of polypeptide components involved in neurite fasciculation and elongation.
Polypeptide components and carbohydrate linkage types of F11 antigen and G4 antigen, two chick cell-surface glycoproteins implicated in neurite fasciculation and elongation [Rathjen, F.G., Wolff, J.M., Bonhoeffer, F. and Rutishauser, U. (1987) J. Cell Biol. 104, 343-353], have been studied in comparison to mouse L1 antigen. Tryptic fingerprint analysis does not reveal any relation of the 130-kDa components of G4 or F11 antigens to each other or to neural cell-adhesion molecules. The 180/190-kDa component of G4 antigen comprises parts of the 130-kDa and 80/65-kDa components and shares a sequence corresponding to the amino terminus of the G4 130-kDa component as shown serologically with anti-peptide sera. This closely parallels the relationship found for mouse L1 antigen components. In contrast, the F11 170-kDa component is different from the F11 130-kDa component, as shown serologically and by fingerprint analysis. A combination of chemical and enzymatic deglycosylation methods reveals that while O-glycosylation cannot be detected F11 130-kDa, G4 130-kDa and L1 140-kDa components contain N-linked carbohydrates. Endoglycosidase H treatment shows that the oligosaccharides present in the G4 130-kDa component and mouse L1 are mostly of the complex type, while the F11 130-kDa component consists of two populations, one containing mainly complex-type carbohydrates and a second containing high-mannose/hybrid-type carbohydrates.
Wolff
J M
JM
Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Federal Republic of Germany.
Rathjen
F G
FG
Frank
R
R
Roth
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000502:physiology; D001369:Axons / Q000502:physiology*; D001923:Brain Chemistry; D002241:Carbohydrates / Q000032:analysis* / Q000276:immunology; D002645:Chickens; D006023:Glycoproteins / Q000032:analysis; D051379:Mice; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis*; D011489:Protein Denaturation; D011817:Rabbits; D014357:Trypsin
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002241:Carbohydrates; D006023:Glycoproteins; D010455:Peptides; D014357:Trypsin