A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures.
A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin-EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up to 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations.
Cheung
S W
SW
Department of Obstetrics and Gynecology, Washington University School of Medicine, Jewish Hospital of St. Louis, Missouri 63110.
Crane
J P
JP
Johnson
A
A
Simms
L
L
Reid
J
J
D000653:Amniotic Fluid / Q000166:cytology*; D002478:Cells, Cultured; D002877:Chromosomes, Human; D005260:Female; D006801:Humans; D008677:Metaphase; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods