Linear response curves from an ELISA assay: measurement of anti-myelin IgG and IgM during experimental allergic neuritis.
An ELISA method for generating linear dose-response curves for measuring anti-myelin IgG and IgM is described. Linearity is achieved without logarithmic transformations. This test is used to measure plasma levels of anti-myelin antibodies in rabbits with EAN. Results are expressed as relative concentrations of antibody in arbitary mass units with 95% confidence limits fitted by inverse prediction. In the 8 animals studied, appearance of anti-myelin antibody always preceded onset of clinical signs and neither anti-myelin IgG nor IgM could be detected in any animal pre-inoculation. Five out of 8 animals displayed steady and progressive increases in anti-myelin IgG with the remaining 3 animals showing plateaus in IgG levels 21-30 days post-inoculation. Increases in anti-myelin IgG were generally parallelled by increases in disease severity. However, 2 animals showed recovery and relapse from EAN with no change in plasma levels of anti-myelin IgG. Patterns of production of anti-myelin IgM varied with some animals displaying early peaks while others showed gradual increases.
Harvey
G K
GK
Department of Medicine, University of Sydney, NSW, Australia.
Schindhelm
K
K
Antony
J H
JH
Pollard
J D
JD
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D009186:Myelin Sheath / Q000276:immunology*; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M