title: Oestrogen binding sites in fresh human aortic tissue.
abstract: Biopsies of aortic tissue were obtained from patients undergoing heart surgery, and were tested for oestrogen binding sites. Tests were carried out using a 7 + 1-point dextran-coated charcoal method, and receptors were quantified both in the cytoplasmic and in the nuclear fractions using Scatchard plot analyses. Tests yielded positive results in 8 cases out of 13 (cytosol fraction) and in 10 cases out of 13 (nuclear fraction). Receptors showed high affinity for oestrogens, the Kd values ranging from 10(-11) M to 10(-10) M. These findings therefore suggest the occurrence of oestrogen receptors in human blood-vessels.
authors: Instituto di Clinica Medica Generale e Terapia Medica I, University of Palermo, Italy.
authors: D
authors: D
authors: Campisi
authors: A
authors: A
authors: Bivona
authors: S
authors: S
authors: Paterna
authors: M
authors: M
authors: Valenza
authors: R
authors: R
authors: Albiero
mesh_terms: D001011:Aorta / Q000378:metabolism; D001158:Arteries / Q000378:metabolism*; D001706:Biopsy; D002467:Cell Nucleus / Q000378:metabolism; D003327:Coronary Disease / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004054:Diethylstilbestrol; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000032:analysis*
chemical_list: D011960:Receptors, Estrogen; D004054:Diethylstilbestrol