title: Determination of total cholesterol in serum by flow injection analysis with immobilized enzymes.
abstract: Several photometric and fluorimetric methods are proposed for the determination of cholesterol by use of enzymes immobilized on controlled-pore glass and the normal and stopped-flow injection modes, achieving linear ranges of the calibration curves between 26-776 mumol/l and 5-265 mumol/l with excellent regression coefficients and good coefficients of variation. The methods have been applied to the determination of this analyte in serum with excellent results.
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
authors: J M
authors: JM
authors: Fernández-Romero
authors: L
authors: L
authors: de Castro
authors: M
authors: M
authors: Valcárcel
mesh_terms: D002784:Cholesterol / Q000097:blood*; D004800:Enzymes, Immobilized; D006801:Humans; D007202:Indicators and Reagents; D010783:Photometry; D013050:Spectrometry, Fluorescence
chemical_list: D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D002784:Cholesterol