title: Primary structure of anti-lipopolysaccharide factor from American horseshoe crab, Limulus polyphemus.
abstract: The complete amino acid sequence of anti-lipopolysaccharide (LPS) factor purified from the hemocytes lysate of the American horseshoe crab, Limulus polyphemus, was determined by characterization of the NH2-terminal sequence and the peptides generated after digestion of the protein with lysyl endopeptidase, clostripain, and Staphylococcus aureus V8 protease. Upon sequencing the peptides by the automated Edman method, the following primary structure was obtained: (Sequence: in text). During the sequence analysis, two species of the protein, which differed from each other at one locus, were found and characterized. L. polyphemus anti-LPS factor was a basic protein consisting of a single polypeptide chain of 101 residues and a calculated molecular weight of 11,786 or 11,800. The hydrophobic NH2-terminal sequence and the clustering of positive charges found in the disulfide loop yielded a typical amphipathic character of this protein. Moreover, L. polyphemus anti-LPS factor showed 83% sequence identity with the Tachypleus tridentatus protein, and the sequence similar to that observed in the EF-hand structure was found to contain in the COOH terminal portions of these proteins, although its function is unknown.
authors: Department of Biology, Faculty of Science, Kyushu University.
authors: T
authors: T
authors: Muta
authors: T
authors: T
authors: Miyata
authors: F
authors: F
authors: Tokunaga
authors: T
authors: T
authors: Nakamura
authors: S
authors: S
authors: Iwanaga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D006737:Horseshoe Crabs / Q000032:analysis*; D007447:Invertebrate Hormones / Q000302:isolation & purification*; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D008070:Lipopolysaccharides; C034705:antilipopolysaccharide factor (Limulus)