The isolation of the two electrophoretic forms of cowpea mosaic virus using fast protein liquid chromatography.
This report describes the first time entire viral capsids have been purified using fast protein liquid chromatography (FPLC) techniques. The FPLC is used here to separate the two electrophoretic forms of cowpea mosaic virus. The capsid forms are shown to be separated by the Mono-Q column without damaging the capsids.
Smith
T J
TJ
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
D002213:Capsid / Q000032:analysis / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D007525:Isoelectric Focusing; D009029:Mosaic Viruses / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal