title: [Isolation of cathepsin D from fish muscle tissue].
abstract: A preparation of cathepsin D was isolated with a 20% yield from the Pacific herring muscles using thermal and acid-alkaline treatment as well as salting out with ammonium sulfate and gel chromatography on Sephadex G-75. Cathepsin D isolated from the Pacific sardine muscles was purified 200-400-fold by means of affinity chromatography on immobilized hemoglobin, the yield being about 5%.
authors: T N
authors: TN
authors: Slutskaia
authors: L A
authors: LA
authors: Igolkina
authors: E V
authors: EV
authors: Sokolova
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D005399:Fishes; D005519:Food Preservation; D009132:Muscles / Q000201:enzymology*
chemical_list: D002402:Cathepsin D
vernacular_title: Vydelenie katepsina D iz myshechnoĭ tkani ryb.