title: [Steroid metabolism in the cellular components of the rat ovarian follicle].
abstract: Steroid metabolism by different cellular components of preovulatory follicles was studied. Granulosa cells(G), theca cells (T), recombination of granulosa and theca cells (G + T), and cut follicles (F) were isolated from proestrus rats. Each component was then incubated for 3-24h with or without androstenedione (5 X 10(-7) M), 14C-androstenedione or 14C-progesterone. Steroid accumulations were measured by RIA. Metabolites of 14C-steroid were separated by TLC and radioassayed. Estradiol was accumulated in a several fold greater amount in F than in G, T or G + T. In 14C-androstenedione metabolites, testosterone was the major metabolite of G and G + T and 5 alpha-androstane-3 alpha, 17 beta-diol was the second metabolite. Androsterone was the major metabolite of T, and 17 beta-OH compounds were detected in small amounts. Androsterone and 5 alpha-androstane-3 alpha, 17 beta-diol were major metabolites of F. In 14C-progesterone metabolites, 5 alpha-pregnane-3 alpha,20 alpha-diol was the major metabolite of G and G + T after the 24h incubation. 5 alpha-pregnane-3 alpha,20 alpha-diol and 3 alpha-hydroxy-5 alpha-pregnane-20-one were the major metabolites of F, and androsterone was also produced. Smaller amounts of 17 beta-OH and 20 alpha-OH compounds were produced in F than in G + T, and 5 alpha-reduced compound production was considerable. Therefore, 17 beta- and 20 alpha-hydroxysteroid dehydrogenase activity were lower in F than in G + T, and/or 5 alpha-reductase activity was higher in F than in G + T.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Obstetrics and Gynecology, Yamagata University School of Medicine.
authors: H
authors: H
authors: Takahashi
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000735:Androstenedione / Q000378:metabolism; D000738:Androsterone / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013799:Theca Cells / Q000378:metabolism
chemical_list: D000728:Androgens; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D000738:Androsterone