Growth of human-human hybridomas in serum-free media enhances antibody secretion.
Human-human hybridomas derived from fusing lymph node lymphocytes with UC 729-6 were adapted to grow in commercially available serum-free medium and were compared with serum-supplemented [10% fetal bovine serum (FBS)] cultures. Over a 6-d period, no significant changes occurred in the growth of the cells in 10% FBS or serum-free medium. In cultures supplemented with 10% FBS more total proteins were secreted than in serum-free cultures. However, there was an enhanced secretion of three- to four-fold of both immunoreactive human IgG and IgM under serum-free conditions compared to serum-supplemented conditions. Serum-free conditions may provide the appropriate milieu for the increased level of Ig secretion from human hybridomas derived from UC 729-6 in that there are no inhibitors that may be present in serum.
Glassy
M C
MC
Biotherapeutics, Inc., La Jolla, California 92037.
Peters
R E
RE
Mikhalev
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001790:Blood Physiological Phenomena; D002417:Cattle; D003470:Culture Media / Q000494:pharmacology*; D006801:Humans; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism*; D007075:Immunoglobulin M / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007074:Immunoglobulin G; D007075:Immunoglobulin M