Revertants of v-fos-transformed fibroblasts have mutations in cellular genes essential for transformation by other oncogenes.
Morphologic revertants of FBJ murine sarcoma virus (v-fos)-transformed rat-1 fibroblasts were isolated using a novel selection procedure based on prolonged retention of rhodamine 123 within mitochondria of v-fos-transformed versus normal fibroblasts. Two classes of revertants were isolated: class I revertants have sustained mutations in cellular genes, and a class II revertant has a nonfunctional v-fos provirus. Somatic-cell hybridization studies suggested that the revertant phenotype was recessive to the transformed phenotype. Class I revertants were also resistant to retransformation by v-gag-fos-fox, v-Ha-ras, v-abl, and v-mos, but could be retransformed by the trk oncogene and polyoma virus middle T antigen. These results suggest that the class I revertants sustained mutations in one or more cellular genes essential for transformation by some, but not all, oncogenes. Our data suggest the existence of common biochemical pathways for transformation.
Zarbl
H
H
Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Quebec, Canada.
Latreille
J
J
Jolicoeur
P
P
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D005347:Fibroblasts / Q000166:cytology; D005796:Genes; D051379:Mice; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification; D009857:Oncogenes; D051381:Rats; D012513:Sarcoma, Experimental / Q000235:genetics / Q000473:pathology*
D009363:Neoplasm Proteins