Effects of plasticizers and plastic bags on granulocyte function during storage.
The influence of the plasticizers, di-(2-ethylhexyl)phthalate (DEHP) and tri-(2-ethylhexyl)trimellitate (TOTM), on granulocyte function was examined. Polyvinyl chloride (PVC) bags with DEHP (DEHP-PVC) leaked DEHP into plasma, but TOTM did not dissolve in plasma under the same conditions. Glow discharge treatment inhibited the leakage of DEHP from DEHP-PVC bags. Depending on the amount of DEHP added into granulocyte suspension, chemotaxis and bactericidal activity decreased, but cell counts and phagocytosis were not affected. During storage for 24 h at 22 degrees C, granulocyte function decreased greatly in DEHP-PVC, but was well maintained in the bags which did not leak plasticizers, TOTM-PVC and glow-discharged DEHP-PVC.
Miyamoto
M
M
Central Blood Center, Japanese Red Cross, Tokyo.
Sasakawa
S
S
D001565:Benzoates / Q000494:pharmacology; D001793:Blood Preservation / Q000295:instrumentation; D004051:Diethylhexyl Phthalate / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D010968:Plasticizers / Q000494:pharmacology*; D011143:Polyvinyl Chloride / Q000494:pharmacology*; D011145:Polyvinyls / Q000494:pharmacology*
D001565:Benzoates; D010968:Plasticizers; D011145:Polyvinyls; C036101:tri-(2-ethylhexyl)trimellitate; D011143:Polyvinyl Chloride; D004051:Diethylhexyl Phthalate