Liquid chromatographic determination of taurine in vitamin premix formulations.
A liquid chromatographic (LC) method is described for the determination of taurine in vitamin and vitamin-mineral premix formulations. The method involves extraction of taurine with 0.1 M bicarbonate buffer, followed by precolumn derivatization with dansyl chloride and LC using fluorescence detection. 6-Aminocaproic acid is used as an internal standard. A reverse phase analytical column and a mobile phase of 0.1 M acetate buffer solution (pH 7.2)-acetonitrile (75 + 25) are used. Vitamins, minerals, and other excipients in the premix formulations do not interfere in the determination. The method is simple, precise, and accurate.
Rao
G N
GN
Abbott Laboratories, Pharmaceutical Products Division, North Chicago, IL 60064.
D002626:Chemistry, Pharmaceutical; D002853:Chromatography, Liquid; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence; D013654:Taurine / Q000032:analysis*; D013696:Temperature; D014815:Vitamins / Q000032:analysis*
D014815:Vitamins; D013654:Taurine