Protein kinase C activity in the spleen of trout (Salmo gairdneri) and the rectal gland of dogfish (Scyliorhinus canicula), and the effects of phosphatidylserine and diacylglycerol containing (n-3) polyunsaturated fatty acids.
1. High speed supernatant fractions of trout spleen and dogfish rectal gland contained 22.5 and 7.2 nmol/min/g tissue of protein kinase C activity respectively. 2. The effect of Ca2+ concentration on the activities with phosphatidylserine (PtdSer) alone, diacylglycerol (DAG) alone and PtdSer and DAG together were determined. Both enzymes required Ca2+ but activity was independent of Ca2+ concentration within the physiological range of 0.1-10 microns. 3. The effect of PtdSer and DAG containing (n - 3) polyunsaturated fatty acids (PUFA) on the activity of protein kinase C from both tissues was examined. Both enzymes were active with all lipids tested and showed little or no discrimination between lipids differing in their contents of (n-3) or (n-6) polyunsaturated fatty acids.
Bell
M V
MV
Department of Biological Science, University of Stirling, Scotland, UK.
Sargent
J R
JR
D000818:Animals; D001921:Brain; D002417:Cattle; D004075:Diglycerides / Q000494:pharmacology*; D004284:Dogfish / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated; D005989:Glycerides / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver; D010718:Phosphatidylserines / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D012487:Salmonidae / Q000378:metabolism*; D012491:Salt Gland / Q000201:enzymology*; D012754:Sharks / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology*; D014337:Trout / Q000378:metabolism*
D004075:Diglycerides; D005231:Fatty Acids, Unsaturated; D005989:Glycerides; D010718:Phosphatidylserines; D011493:Protein Kinase C