Effects of glycyrrhizin and glycyrrhetinic acid on growth and melanogenesis in cultured B16 melanoma cells.
The effects of glycyrrhizin (GL) and its aglycone, glycyrrhetinic acid (GA), on the growth and differentiation of mouse melanoma (B16) cells in culture were studied. GA inhibits the growth of B16 melanoma cells, causes morphological alterations and stimulates melanogenesis. GL also resulted in the same changes but only when the concentration was about 20 times more than that needed for GA. When GA was removed after 84 h of treatment, the growth rate recovered slightly, but the doubling time was about twice that of the control. Cytofluorometric analysis showed that the growth inhibition of GA is the result of inhibition of the transfer from G1 to S phase.
Abe
H
H
Research Institute of Oriental Medicine, Kinki University, Osaka, Japan.
Ohya
N
N
Yamamoto
K F
KF
Shibuya
T
T
Arichi
S
S
Odashima
S
S
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D019695:Glycyrrhizic Acid; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism
D008543:Melanins; D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid