Colorimetric-detected DNA sequencing.
A sensitive, colorimetric method for visualizing the band pattern of DNA sequencing reaction is described. The enzymatic incorporation of radioactive nucleotides commonly used for the band detection is replaced by biotin conjugated to the 5'-terminus of a synthetic oligonucleotide by chemical synthesis. The oligonucleotide so labeled is used as a primer for dideoxy DNA sequencing in a primer extension reaction. The products of the sequencing reactions are analyzed on a denaturing polyacrylamide gel using the direct blotting electrophoresis technique. This technique makes it possible to transfer the band pattern during the electrophoresis onto an immobilizing matrix, on which it is made visible by an enzymatic reaction in less than 3 h. This biotin-based detection method is so sensitive that the sequencing reactions can be performed under the same conditions and concentrations as those for the radioactive detection.
Beck
S
S
Medical Research Council, Laboratory of Molecular Biology, Cambridge, England.
D001426:Bacterial Proteins; D001483:Base Sequence; D001710:Biotin; D003124:Colorimetry / Q000379:methods*; D004247:DNA; D004586:Electrophoresis / Q000379:methods; D019809:Streptavidin
D001426:Bacterial Proteins; D001710:Biotin; D004247:DNA; D019809:Streptavidin