A novel method to investigate the heterocliticity of antibodies.
Monoclonal and anti-dinitrophenyl and anti-trinitrophenyl IgE antibodies were used to measure heterocliticity using competitive inhibition assays with homologous and heterologous haptens. The antibodies or antibody-containing ascites fluids were diluted to give 50% of the maximum binding to wells of antigen-coated microtiter plates. The % inhibition of binding of the antibody to the antigen by various concentrations of homologous and heterologous haptens at a standard dilution of antibody can then be compared. The advantages of this method of determination of heterocliticity are that it is fast, simple, quantitative and does not need radiolabeled reagents.
Furusawa
S
S
New York University Medical School, Department of Pathology, NY 10016.
Ovary
Z
Z
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D000918:Antibody Specificity; D004136:Dinitrobenzenes / Q000276:immunology*; D006241:Haptens; D007073:Immunoglobulin E / Q000276:immunology; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology*
D000910:Antibodies, Heterophile; D004136:Dinitrobenzenes; D006241:Haptens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D007073:Immunoglobulin E