The mode of action of some benzimidazole drugs on Trichinella spiralis.
In an attempt to find possible targets for benzimidazole action in muscle-stage larvae of Trichinella spiralis, the effects of mebendazole and thiabendazole were tested in vivo by oral treatment of infested mice and in vitro by including these anthelmintics in an adequate maintenance medium containing decapsulated larvae. The effects of the anthelmintics on succinate dehydrogenase and fumarate reductase activities, measured in the mitochondrial fraction obtained from the in vivo- or in vitro-treated larvae showed that only thiabendazole causes significant inhibition of fumarate reductase activity. On the other hand, measurements of free glucose, glycogen reserves and soluble protein in the treated larvae indicate that in vivo, mebendazole and thiabendazole clearly diminish free glucose levels, although in vitro only mebendazole produces the same diminution. Both the glycogen and protein contents of the larvae remained unchanged after treatment in vivo or in vitro. The importance of these findings with regard to a possible site of action for mebendazole and thiabendazole is discussed.
Criado Fornelio
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Department of Parasitology, Faculty of Pharmacy, University of Alcalá de Henares, Spain.
Rodriguez Caabeiro
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Jimenez Gonzalez
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D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D008463:Mebendazole / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D011506:Proteins / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013827:Thiabendazole / Q000494:pharmacology* / Q000627:therapeutic use; D014234:Trichinella / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D014235:Trichinellosis / Q000188:drug therapy* / Q000469:parasitology
D011506:Proteins; D008463:Mebendazole; D006003:Glycogen; D013385:Succinate Dehydrogenase; D005947:Glucose; D013827:Thiabendazole; D003078:Colchicine