The probability with which EMS-initiated mutagenic lesions generate mutations in CHO cells.
In this note we distinguish between multiple mutations affecting a given locus which are generated at separate error-prone lesions and multiple independent mutations generated at a single error-prone lesion. We describe a basis for determining the probability with which the latter class of mutations occurs based on the mutant fraction in the progeny and determine an average probability of 0.6 mutations/replication/mutagenic site for those EMS-modified sites which are mutagenic for G6PDH activity in CHO cells.
Aronson
J F
JF
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Stamato
T D
TD
D000818:Animals; D006224:Cricetinae; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects*; D011336:Probability
D004247:DNA; D005020:Ethyl Methanesulfonate