Fatty acid acylation of proteins in Physarum polycephalum.
We have investigated the occurrence of protein-fatty acid acylation by metabolic incorporation of [3H]myristic and [3H]palmitic acids in Physarum polycephalum. We show that this organism contains fatty acylated proteins with mainly myristic acid covalently attached in alkali-stable linkages, probably amides. We find no evidence for ester-linked fatty acids, in contrast to the situation in vertebrate cells.
Monteiro
M
M
National Institute for Medical Research, Mill Hill, London, U.K.
Magee
A I
AI
D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000378:metabolism*; D008970:Molecular Weight; D009227:Myristic Acids / Q000378:metabolism; D010169:Palmitic Acids / Q000378:metabolism; D010804:Physarum / Q000201:enzymology / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
D005227:Fatty Acids; D009227:Myristic Acids; D010169:Palmitic Acids; D011506:Proteins; D000217:Acyltransferases