Aminoglycoside resistance patterns in clinical isolates of Enterobacteriaceae from Czechoslovakia.
Multi-resistant Enterobacteriaceae isolated mainly from urine specimens from patients at the Department of Urology, Kramáre Hospital, Bratislava, were characterized for resistance phenotype. Seventeen gentamicin-resistant isolates were further studied for the presence of aminoglycoside-modifying enzymes. Five enzymes were detected: AAC(2'), AAC(3)-II, AAC non-characterized, ANT(2") and APH(3')-I. The substrate range of these enzymes was found to correlate with the resistance phenotype in most isolates. In our collection the AAC(3)-II enzyme that inactivates gentamicin, sisomicin, tobramycin and netilmicin was predominant. Predominance of this type of modifying enzyme has been observed also in resistant Gram-negative strains in Belgium, The Netherlands and Chile, in contrast to the United States, Federal Republic of Germany, Switzerland, Greece and Turkey, where ANT(2") has been the most common enzyme.
Kettner
M
M
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Navarová
J
J
Langsádl
L
L
D000123:Acetyltransferases / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001437:Bacteriuria / Q000382:microbiology*; D003604:Czechoslovakia; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D000123:Acetyltransferases