Diurnal rhythm of vasopressin but not of oxytocin in the cerebrospinal fluid of the goat: lack of association with plasma cortisol rhythm.
Diurnal rhythms in the concentrations of vasopressin (AVP) and oxytocin in cerebrospinal fluid (CSF) differ between species and are unrelated to changes in the levels of these hormones in the peripheral circulation. We have investigated neurohypophysial hormone rhythms in the CSF of the conscious goat by determining whether they are entrained by daily cycles of light and darkness and by assessing the effect of the suppression of plasma cortisol. Goats were implanted with cisternal cannulae under halothane anaesthesia and allowed to recover. They were accustomed to a 12 h light:12 h darkness lighting cycle (lights on from 07.30 to 19.30 h). After initial serial CSF and plasma sampling the daily cycle of light and darkness was reversed. Three goats were kept in constant light for 8 days before the study and five were given dexamethasone (5 mg/12 h) for 4 days. There was a significant (P less than 0.01) diurnal variation in CSF concentrations of AVP, with a maximum of 3.6 +/- 0.8 (S.E.M.) pmol/l at 12.00 h and a minimum of 1.4 +/- 0.3 pmol/l at 24.00 h. There were no significant changes in CSF concentrations of oxytocin or plasma AVP. After the light:darkness cycle was reversed the AVP rhythm in the CSF was disrupted after 24 h and reversed after 8 days. The diurnal rhythm of AVP in CSF persisted in animals exposed to constant light. After treatment with dexamethasone plasma cortisol was suppressed and showed no diurnal rhythm but the AVP rhythm in CSF remained unchanged.
Seckl
J R
JR
Department of Medicine, Charing Cross and Westminster Medical School, Westminster Hospital, London.
Lightman
S L
SL
D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood / Q000134:cerebrospinal fluid*; D002940:Circadian Rhythm / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D006041:Goats / Q000502:physiology*; D006854:Hydrocortisone / Q000097:blood; D008027:Light; D010121:Oxytocin / Q000134:cerebrospinal fluid
D001127:Arginine Vasopressin; D010121:Oxytocin; D003907:Dexamethasone; D006854:Hydrocortisone