Identification of Bacteroides species from adult periodontal disease.
Samples from deep (4-7 mm) periodontal pockets were collected from 17 patients with adult-type periodontal disease and one with the juvenile form of the disease. They were streaked immediately on selective and non-selective media and incubated anaerobically for 96 h. There was a heavy growth of Bacteroides spp. from most samples and 10 representative colonies from each sample were sub-cultured for identification. In a total of 149 isolates from patients with adult-type disease, the commonest species were B. oralis (40), B. asaccharolyticus (35), B. intermedius (31), B. fragilis (12) and B. ureolyticus (10); B. gingivalis was not detected. The distribution of species was not distorted by multiple identical isolates from individual patients. There was a heavy growth of a single species, B. ureolyticus, from the patient with juvenile-type disease.
Duerden
B I
BI
Department of Medical Microbiology, University of Sheffield Medical School.
Goodwin
L
L
O'Neil
T C
TC
D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000382:microbiology; D001439:Bacteroides / Q000254:growth & development / Q000302:isolation & purification*; D001442:Bacteroides Infections / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology*