title: Both near ultraviolet radiation and the oxidizing agent hydrogen peroxide induce a 32-kDa stress protein in normal human skin fibroblasts.
abstract: We have analyzed the pattern of protein synthesis in solar near ultraviolet (334 nm, 365 nm) and near visible (405 nm) irradiated normal human skin fibroblasts. Two hours after irradiation we find that one major stress protein of approximately 32 kDa is induced in irradiated cells. This protein is not induced by ultraviolet radiation at wavelengths shorter than 334 nm and is not inducible by heat shock treatment of these cells. Although sodium arsenite, diamide, and menadione all induced a 32-kDa protein, they also induced the major heat shock proteins. In contrast, the oxidizing agent, hydrogen peroxide, induced the low molecular weight stress protein without causing induction of the major heat shock proteins. A comparison of the 32-kDa proteins induced by sodium arsenite, H2O2, and solar near ultraviolet radiation using chemical peptide mapping shows that they are closely related. These results imply that the pathways for induction of the heat shock response and the 32-kDa protein are not identical and suggest that, at least in the case of radiation and treatment with H2O2, the 32-kDa protein might be induced in response to cellular oxidative stress. This conclusion is supported by the observation that depletion of endogenous cellular glutathione prior to solar near ultraviolet irradiation lowers the fluence threshold for induction of the 32-kDa stress protein.
authors: Swiss Institute for Experimental Cancer Research, Epalinges/Lausanne.
authors: S M
authors: SM
authors: Keyse
authors: R M
authors: RM
authors: Tyrrell
mesh_terms: D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005978:Glutathione / Q000502:physiology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D018053:Arsenites; D006360:Heat-Shock Proteins; D006861:Hydrogen Peroxide; D005978:Glutathione; C015001:arsenite; D001151:Arsenic