Hypoxanthine transport through the blood-brain barrier.
The unidirectional influx of hypoxanthine across cerebral capillaries, the anatomical locus of the blood-brain barrier, was measured with an in situ rat brain perfusion technique employing [3H]hypoxanthine. Hypoxanthine was transported across the blood-brain barrier by a saturable system with a one-half saturation concentration of approximately 0.4 mM. The permeability-surface area product was 3 X 10(-4) sec-1 with a hypoxanthine concentration of 0.02 microM in the perfusate. Adenine (4 mM) and uracil and theophylline (both 10 mM), but not inosine (10 mM) or leucine (1 mM), inhibited hypoxanthine transfer through the blood-brain barrier. Thus, hypoxanthine is transported through the blood-brain barrier by a high-capacity, saturable transport system with a half-saturation concentration about 100 times the plasma hypoxanthine concentration. Although involved in the transport hypoxanthine from blood into brain, this system is not powerful enough to transfer important quantities of hypoxanthine from blood into brain.
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Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242.
D000225:Adenine / Q000494:pharmacology; D000818:Animals; D001812:Blood-Brain Barrier; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism*; D007288:Inosine / Q000494:pharmacology; D007930:Leucine / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013806:Theophylline / Q000494:pharmacology; D013831:Thiamine / Q000494:pharmacology; D014498:Uracil / Q000494:pharmacology
D007042:Hypoxanthines; D019271:Hypoxanthine; D014498:Uracil; D007288:Inosine; D013806:Theophylline; D007930:Leucine; D000225:Adenine; D011339:Probenecid; D013831:Thiamine