title: Tissue distribution and molecular heterogeneity of bovine thiol:protein-disulphide oxidoreductase (disulphide interchange enzyme).
abstract: 1. The distribution of thiol:protein-disulphide oxidoreductase (disulphide interchange enzyme) in 17 bovine tissue extracts was determined by rocket immunoelectrophoresis and by measuring the reductive cleavage of insulin. 2. The relative concentration (per mg total protein) was found to be in the order: Pancreas greater than liver greater than lymph node greater than testes, fat tissue greater than parotid gland, brain, spleen, lung greater than small intestine, spinal cord, large intestine, kidney greater than paunch, aorta greater than skeletal muscle greater than heart. 3. The distribution of specific activity showed a similar pattern, irrespectively of whether glutathione or L-cysteine was used as cosubstrate. 4. The concentration varied 200-fold and the specific activity 400-fold between pancreas and heart muscle, respectively. 5. Crossed immunoelectrophoresis demonstrated that a fast-migrating form of the enzyme was the only one present in almost all tissues, but 15% of the enzyme in liver was a slow-migrating form and 50% in heart muscle a medium-migrating form. 6. The lung contains a species having partial immunological identity to the enzyme. 7. Purified enzyme from bovine liver has a somewhat lower mobility than the fast-migrating form in extract. 8. The results seem to support the general view that the enzyme is involved in synthesis of disulphide-bonded extracellular proteins, although the presence of the enzyme in tissues like fat, brain, spinal cord, skeletal muscle and heart indicates other cellular functions as well.
authors: Finsen Laboratory, Finsen Institute, Copenhagen, Denmark.
authors: S
authors: S
authors: Bjelland
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007527:Isoenzymes / Q000378:metabolism*; D007535:Isomerases / Q000378:metabolism*; D008297:Male; D019704:Protein Disulfide-Isomerases; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D007535:Isomerases; D019704:Protein Disulfide-Isomerases