Activated N-ras oncogene in a transformant derived from a rat small intestinal adenocarcinoma induced by 2-aminodipyrido[1,2-a:3',2'-d]imidazole.
DNAs from five intestinal adenocarcinomas induced by 2-aminodipyrido[1,2-a:3',2'-d]imidazole, which is present in broiled fish, were subjected to transfection assay using NIH3T3 cells as recipients. The DNA from only one adenocarcinoma induced a morphologically transformed focus. Rat N-ras sequences were detected in the primary transformant and in three tested secondary transformants. In the activated N-ras oncogene, a G----T transversion at the first letter of codon 12 was detected. The original tumor DNA did not hybridize with the oligonucleotide representing the mutated allele, but did hybridize with the one representing the normal allele. From these data we concluded either that the activation of the N-ras oncogene had occurred during the transfection or that the activated N-ras oncogene had been present in a minor population of cells in the original tumor.
Ishizaka
Y
Y
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Ochiai
M
M
Ishikawa
F
F
Sato
S
S
Miura
Y
Y
Nagao
M
M
Sugimura
T
T
D000230:Adenocarcinoma / Q000139:chemically induced / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000032:analysis; D007093:Imidazoles / Q000633:toxicity; D007414:Intestinal Neoplasms / Q000139:chemically induced / Q000235:genetics; D009154:Mutation; D011519:Proto-Oncogenes; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection
D004273:DNA, Neoplasm; D007093:Imidazoles; C023847:Glu-P-2