A proteolytic 140-kDa fragment of neurite outgrowth factor and its cell-binding activity.
A neurite outgrowth factor (NOF) from chicken gizzard was treated with elastase, and a protease-resistant fragment of 140 kDa (F-140) with heparin-binding activity, was isolated from the digested mixture. Although F-140 showed no neurite outgrowth activity, it could bind dissociated cells from the dorsal root ganglia of chick embryos. The antiserum to F-140 inhibited the cell binding activity of native NOF in a dose-dependent manner, but not the neurite outgrowth activity. When membrane proteins from whole chick embryos were incubated with NOF or F-140, a protein band of 41 kDa was detected as a major NOF-binding component.
Hayashi
Y
Y
Department of Pharmacology, Kanazawa University, Japan.
Taniura
H
H
Miki
N
N
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002645:Chickens; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology; D007106:Immune Sera / Q000276:immunology; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D009474:Neurons / Q000502:physiology; D010196:Pancreatic Elastase; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D007106:Immune Sera; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D010196:Pancreatic Elastase