Continuous chromogenic tissue factor assay: comparison to clot-based assays and sensitivity established using pure tissue factor.
The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor. Log-log plots of the clot-times versus A405/min2 are linear over the mutually useful ranges of tissue factor concentration. Using human placental tissue factor reconstituted into phosphatidylserine-phosphatidylcholine (30:70) vesicles, the chromogenic assay quantitatively detected tissue factor in dilutions containing from 3 femtomoles to less than 10 attomoles of protein.
Carson
S D
SD
Department of Pathology, University of Colorado, School of Medicine, Denver 80262.
D000818:Animals; D001780:Blood Coagulation Tests; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002863:Chromogenic Compounds / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008722:Methods; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D013925:Thromboplastin / Q000032:analysis*; D014914:Whole Blood Coagulation Time
D002863:Chromogenic Compounds; D013925:Thromboplastin