[Preparation of monospecific antisera to IgA in laboratory animals using cascade immunization without preliminary isolation of the antigen].
The proposed method of "cascade" immunization for the preparation of monospecific antisera to IgA of the experimental animals (mice, rats, guinea-pigs) requires no isolation and purification of an antigen, since it is based on using precipitation lines containing IgA. The subsequent steps of the method are the following: 1) preparation of a polyspecific antiserum against one of the secretions or IgA-containing serum fraction; 2) preparation of precipitation lines using this antiserum and some other secretions; 3) immunization of other rabbits with these precipitation lines to obtain monospecific anti-IgA antiserum. The antisera obtained do not require any additional absorption except for the removal of anti-IgA antibodies. The method can be used for the preparation of monospecific antisera to any other serum or secretion proteins.
Liubinskaia
M M
MM
Chernokhvostova
E V
EV
D000818:Animals; D000918:Antibody Specificity; D006168:Guinea Pigs; D007106:Immune Sera; D007114:Immunization / Q000379:methods; D007070:Immunoglobulin A / Q000276:immunology*; D051379:Mice; D051381:Rats
D007106:Immune Sera; D007070:Immunoglobulin A
Poluchenie monospetsificheskikh antisyvorotok k IgA laboratornykh zhivotnykh metodom kaskadnoĭ immunizatsii bez predvaritel'nogo vydeleniia antigena.