Factors affecting the production of SCEs by maleic hydrazide in root-tip chromosomes of Allium cepa.
We have investigated the influence of pH on the induction of chromatid-type aberrations and sister-chromatid exchanges (SCEs) by maleic hydrazide (MH) in root-tip cells of Allium cepa. For both cytogenetic endpoints, the lower the pH of the treatment solution, the higher were the frequencies of chromosome alterations detected at metaphase. We have further studied the persistence of lesions giving rise to SCEs during successive cell cycles, as well as the influence of BrdU concentration in the post-treatment medium on the yield of MH-induced SCEs. Our results suggest that the cytogenetic action of MH in many respects resembles that of bifunctional alkylating agents.
Cortés
F
F
Departamento de Citología e Histología Vegetal y Animal, Facultad de Biología, Sevilla, Spain.
Escalza
P
P
Mateos
S
S
Díaz-Recasens
M
M
D000490:Allium / Q000235:genetics; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle; D002869:Chromosome Aberrations; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D008300:Maleic Hydrazide / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D011724:Pyridazines; D001973:Bromodeoxyuridine; D008300:Maleic Hydrazide