title: Myosin isoforms in normal and dystrophic chickens.
abstract: The myosin isoform content in the affected fibers of chickens with inherited muscular dystrophy has been investigated with a new high-performance liquid chromatographic procedure for separation of the tryptic fragments of myosin subfragment 1 (S-1). The results indicate that dystrophic muscle contains substantial amounts of normal adult myosin, together with various myosin species present in normal 5-day posthatch chickens. Confirmation was obtained by comparative peptide mapping of the S-1 tryptic fragments and by N-terminal sequencing of 20-kDa species. Together with data on other contractile proteins and certain metabolic enzymes [Obinata, T., Takano-Ohmura, H., & Matsuda, R. (1980) FEBS Lett. 120, 195-198; Mikasa, T., Takeda, S., Shimizu, T., & Kitaura, T. (1981) J. Biochem. (Tokyo) 89, 1951-1962; Feit, H., & Domke, R. (1982) Cell Motil. 2, 309-315; Cosmos, E. (1966) Dev. Biol. 13, 163-181; Cosmos, E., & Butler, J. (1967) in Exploratory Concepts in Muscular Dystrophy and Related Disorders (Milhorat, A. R., Ed.) pp 197-204, Excerpta Medica, Amsterdam], the results are consistent with the hypothesis that there is a general defect in muscle maturation in avian dystrophy.
authors: Department of Biochemistry, State University of New York, Brooklyn 11203.
authors: J I
authors: JI
authors: Rushbrook
authors: C
authors: C
authors: Weiss
authors: A G
authors: AG
authors: Wadewitz
authors: A
authors: A
authors: Stracher
authors: A G
authors: AG
authors: Wadevitz
mesh_terms: D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D008970:Molecular Weight; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism*; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D009218:Myosins / Q000235:genetics* / Q000302:isolation & purification; D010449:Peptide Mapping; D012016:Reference Values
chemical_list: D009218:Myosins