title: Cytokinesis is defective in Dictyostelium mutants with altered phagocytic recognition, adhesion, and vegetative cell cohesion properties.
abstract: Mutants that have been selected for defects in phagocytic recognition, adhesion, and vegetative cell-cell cohesion were found to be larger and more highly multinucleate than their parent strain. This defect is associated with the complex mutant phenotype of these mutants since revertants of the mutants coordinately acquire the wild-type phenotype for all of the defects. The larger size and multinuclearity were due to a high frequency of failure of cytokinesis in cells of wild-type size. This was shown by purifying the small cells in mutant populations and observing their growth and cell division. The mutant phenotype is more penetrant during axenic growth. Most of the mutants are not multinucleate when grown on bacteria. Recently, new mutants have been isolated that are also multinucleate when grown on bacteria by a strong selection procedure for non-adhesion to tissue culture dishes. The pleiotropic mutant phenotype and the greater penetrance of the mutant phenotype in axenic culture can be explained by hypothesizing a deficiency in a membrane component of the actomyosin motor that is involved in all of the processes defective in the mutants.
authors: Bergische Universität GHS, Wuppertal, Federal Republic of Germany.
authors: D R
authors: DR
authors: Waddell
authors: K
authors: K
authors: Duffy
authors: G
authors: G
authors: Vogel
mesh_terms: D002467:Cell Nucleus / Q000648:ultrastructure; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D010587:Phagocytosis