Ketone bodies inhibit leucine degradation in chick skeletal muscle.
1. DL-beta-hydroxybutyrate (4 mM) increased the net rate of leucine transamination and the net rate of 2-oxoisocaproate (KIC) production in extensor digitorum communis muscles from fed chicks. 2. DL-beta-hydroxybutyrate at 1 and 4 mM inhibited leucine oxidative decarboxylation in muscles from fed chicks. 3. Acetoacetate at 1 and 4 mM inhibited leucine oxidative decarboxylation and total leucine oxidation, but increased net KIC production in muscles from fed chicks. 4. Both DL-beta-hydroxybutyrate and acetoacetate at 1 and 4 mM inhibited the net rate of leucine transamination and the rates of leucine oxidative decarboxylation and total leucine oxidation in muscles from 24-hr fasted chicks.
Wu
G
G
Department of Animal Science, University of Alberta, Edmonton, Canada.
Thompson
J R
JR
D000090:Acetoacetates / Q000494:pharmacology; D000818:Animals; D002642:Chick Embryo; D005215:Fasting; D007657:Ketone Bodies / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*
D000090:Acetoacetates; D007657:Ketone Bodies; D007930:Leucine