Analysis of paired serum, urine and filter paper blood specimens for presence of filarial antigen by immunoradiometric assay.
Paired serum, urine, and finger-prick whole blood dried on filter paper were analyzed by immunoradiometric assay (IRMA) for filarial antigen using Brugia malayi-specific rabbit antibody. Nine sera and 6 urines from the 10 paired serum-urine samples obtained from individuals with microfilaremia contained IRMA detectable filarial antigen. In contrast, all serum and urine specimens from patients with chronic infections, endemic and non-endemic controls were negative. Whole blood eluted from filter paper spots contained IRMA detectable material; their degree of positivity agreed well with IRMA binding levels obtained with paired urines. Reduced recovery of antigen dried on filter paper was observed at antigen levels less than 10 ng/ml equivalents, presumably due to irreversible absorption onto the filter paper. Urine and finger-prick filter paper blood specimens can be used in the diagnosis of microfilaremic infections that have been associated with circulating antigen in the blood.
Prasad
G B
GB
Mahatma Gandhi Inst. Med. Sciences, Dept. Biochem., Maharashtra, India.
Harinath
B C
BC
Hamilton
R G
RG
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000097:blood* / Q000652:urine; D005370:Filarioidea / Q000276:immunology*; D006801:Humans; D011863:Radioimmunoassay
D000947:Antigens, Helminth