Calcium signals in neutrophils can be divided into three distinct phases.
Rabbit neutrophils were loaded with the fluorescence probe indo-1 and cytosolic free calcium levels were monitored during chemotactic peptide (fMet-Leu-Phe) stimulation. The fMet-Leu-Phe-induced calcium signal consisted of three consecutive phases: (1) an initial peak that was independent of extracellular calcium, (2) a secondary shoulder that required extracellular calcium but was totally blocked by hyperosmolality and (3) a final plateau of elevated calcium that was dependent on extracellular calcium but insensitive to hyperosmolality.
Chandler
D E
DE
Department of Zoology, Arizona State University, Tempe 85287.
Kazilek
C J
CJ
D000818:Animals; D002118:Calcium / Q000097:blood*; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007211:Indoles; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits
D005456:Fluorescent Dyes; D007211:Indoles; D009240:N-Formylmethionine Leucyl-Phenylalanine; C048960:indo-1; D002118:Calcium