title: A clinical evaluation of the Cobas Fara clinical chemistry analyzer for some routine serum enzymes and glucose.
abstract: The authors evaluated the Cobas FARA centrifugal analyzer with respect to pipetting precision and accuracy, instrument temperature, spectrophotometric response, and analytic performance for the assay of five serum enzymes and glucose. Spectrophotometric response, temperature response, pipetting precision, and accuracy were satisfactory. However, sufficient time must be allowed for cuvet contents to reach a stable temperature before measurements are made. Total day-to-day imprecision (within plus between run) was less than 5% (coefficient of variation) for aspartate and alanine aminotransferases (AST; Enzyme Commission classification number [EC] EC 2.6.1.1; and ALT; EC 2.6.1.2); alkaline phosphatase (AP; EC 3.1.3.1); gamma-glutamyltransferase (GGT; EC 2.3.1.2); lactate dehydrogenase (LD; EC 1.1.1.17); creatine kinase (CK; EC 2.7.3.1); and glucose assays. Results compare well with those obtained with other current clinical chemistry analyzers; correlation coefficients were greater than 0.993. Sample-to-sample carryover was negligible, and method linearity was satisfactory for all tests.
authors: Department of Clinical Biochemistry, University Hospital, London, Ontario, Canada.
authors: G C
authors: GC
authors: Moses
authors: G O
authors: GO
authors: Lightle
authors: J F
authors: JF
authors: Tuckerman
authors: A R
authors: AR
authors: Henderson
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D002498:Centrifugation / Q000295:instrumentation; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004798:Enzymes / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans
chemical_list: D001786:Blood Glucose; D004798:Enzymes