Production of monoclonal antibodies against rice dwarf virus and characterization of antigenic proteins with affinity media.
Hybridomas were developed which secreted antibodies to the core and outer capsids of rice dwarf virus (RDV). The reactive proteins of RDV were simply and rapidly analyzed using monoclonal antibody (MoAb)-coupled Affi-Gel 701 beads and SDS polyacrylamide gel electrophoresis and were isolated with a MoAb-coupled Sepharose 4B affinity column.
Kitagawa
Y
Y
Laboratory of Plant Gene Engineering, Akita Prefectural College of Agriculture, Japan.
Okuhara
E
E
Shikata
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006825:Hybridomas; D012275:Oryza / Q000382:microbiology; D010942:Plant Viruses / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins