Comparative study on the therapeutic ultrasound effects on erythrocyte membrane-bound and free acetylcholinesterase.
Kinetics of erythrocyte acetylcholinesterase activity alterations exposed to ultrasound of therapeutic intensities of 0.88 MHz and 0.05-1.5 W/cm2 was studied. The differences were studied between the mechanisms of the inactivation of membrane-bound and free enzyme: the diminution of active enzyme sites for membrane-bound acetylcholinesterase and the decrease of enzyme-substrate affinity for the free form during sonication. The combined mechanical stresses in the ultrasonic field did not produce inactivation of free enzyme, as compared to the membrane-bound enzyme. Exponential ultrasonic/acoustochemical inactivation curves were obtained for the soluted crystalline form of acetylcholinesterase.
Braginskaya
F I
FI
Institute of Chemical Physics, Academy of Sciences USSR, Moscow.
Zorina
O M
OM
D000110:Acetylcholinesterase / Q000097:blood*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008954:Models, Biological; D012995:Solubility; D014464:Ultrasonic Therapy / Q000009:adverse effects*
D000110:Acetylcholinesterase