IgM response and resistance to ascites tumor growth.
Antibody response and protection against Ehrlich ascites tumor (EAT) was studied in eight EAT-immunized strains of mice (AL/N, BALB/C, C57BL/6J, F1 (C57BL/6 x BALB/C), C57BL/10J, B10.BR, CBA/Ca, SW). The results showed a close association between IgM response and resistance to subsequent tumor challenge. Thus, protection was only achieved in those animals giving a measurable IgM response against EAT cell surface antigens, i.e., all inbred strains of mice tested, except CBA/Ca, and some outbred SW mice. The lack of IgM response to these antigens in CBA/Ca was not linked to the strain H-2 haplotype. Resistance could be passively transferred to nonimmunized mice by means of serum, or purified IgM, from protected immune animals. Moreover, complement depletion by cobra venom factor treatment did not modify the protection afforded to those mice. IgM reactivity to EAT cells was completely abolished by previous cell trypsinization. Trypsin removed but did not destroy the antigen(s) recognized by the IgM, since all its activity could be absorbed with the supernatant of the EAT cell trypsinization. Absorption assays with this supernatant treated with different agents, showed that lipids, simple peptides and nucleic acids were not important components of the antigenic determinants. On the contrary, its susceptibility to beta-galactosidase and particularly to a mild periodate oxidation, suggested that determinants recognized by the IgM against the EAT cell surface are carbohydrate in nature.
Subiza
J L
JL
Servicio de Immunologia, Hospital Clínico de San Carlos, Madrid, Spain.
Coll
J
J
Alvarez
R
R
Valdivieso
M
M
de la Concha
E G
EG
D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D002241:Carbohydrates / Q000276:immunology; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology* / Q000473:pathology; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D007116:Immunization, Passive; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D002241:Carbohydrates; D007075:Immunoglobulin M; D003165:Complement System Proteins