Electrochemically treated glassy carbon electrode for amperometric detection in high-performance liquid chromatography.
The application of an electrochemically pre-treated glassy carbon electrode for the amperometric detection of electroactive components, such as tyrosine and oxipurinol, in biological samples was studied in order to demonstrate the usefulness of a pre-anodized electrode in high-performance liquid chromatography. The electrochemical pre-treatment was carried out in 0.2 M phosphate buffer (potassium dihydrogenphosphate-potassium hydroxide, pH 6.5) at 1900 mV vs. Ag/AgCl for 2 min. The pre-anodized electrode response for the oxidation of lactic acid and pyruvic acid was also studied. The electrochemical treatment enhanced and stabilized the electrode response to the oxidation of tyrosine and both acids.
Iriyama
K
K
Division of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
Iwamoto
T
T
Yoshiura
M
M
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004566:Electrodes; D006073:Gout / Q000097:blood; D006801:Humans; D007773:Lactates / Q000032:analysis / Q000097:blood; D019344:Lactic Acid; D010117:Oxypurinol / Q000032:analysis / Q000097:blood; D010710:Phosphates; D011773:Pyruvates / Q000032:analysis / Q000097:blood; D019289:Pyruvic Acid; D006435:Renal Dialysis; D014443:Tyrosine / Q000032:analysis / Q000097:blood
D007773:Lactates; D010710:Phosphates; D011773:Pyruvates; D019344:Lactic Acid; D014443:Tyrosine; D019289:Pyruvic Acid; D010117:Oxypurinol