Sensitive ferrocene reagents for derivatization of thiol compounds in high-performance liquid chromatography with dual-electrode coulometric detection.
Three N-substituted maleimides possessing ferrocene as an electrophore were prepared and evaluated for pre-column derivatization of thiol compounds in high-performance liquid chromatography with dual-electrode coulometric detection. The utility of these reagents was investigated by employing N-acetyl-L-cysteine as a model compound. Among the three, N-(ferrocenyl) maleimide was the most favourable reagent with respect to reactivity, stability and electrochemical properties. The dual-electrode coulometric detection of the adduct showed high selectivity and sensitivity with a detection limit of 0.06 pmol. The proposed method is applicable to the determination of glutathione in biological specimens.
Shimada
K
K
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Oe
T
T
Nambara
T
T
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005296:Ferrous Compounds; D005978:Glutathione / Q000032:analysis / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis; D008297:Male; D000075163:Metallocenes; D009942:Organometallic Compounds; D013438:Sulfhydryl Compounds / Q000032:analysis*
D005296:Ferrous Compounds; D007202:Indicators and Reagents; D000075163:Metallocenes; D009942:Organometallic Compounds; D013438:Sulfhydryl Compounds; D005978:Glutathione; C004998:ferrocene