Effectiveness of a Prudhoe Bay crude oil and its aliphatic, aromatic and heterocyclic fractions in inducing mortality and aryl hydrocarbon hydroxylase in chick embryo in ovo.
Prudhoe Bay crude oil (PBCO) and its aliphatic, aromatic and heterocyclic fractions were tested on the developing chick embryo for (i) embryotoxicity (ii) their ability to induce hepatic and renal cytochrome P450 levels as well as hepatic, renal and pulmonary aryl hydrocarbon hydroxylase activities. On the basis of its concentration in PBCO, the aromatic fraction was responsible for most of the embryotoxicity as well as for the enzyme inducing ability. The NOS fraction constituted less than 7% (w/v) of PBCO but, on a weight equivalent basis, was roughly as potent as the aromatic fraction in causing embryotoxicity and in inducing cytochrome P450 levels and aryl hydrocarbon hydroxylase. The aliphatic fraction was found to be essentially inactive. The results are consistent with the concept that elevation of aryl hydrocarbon hydroxylase levels by certain components of PBCO may lead to increased embryotoxicity.
Walters
P
P
Biochemistry Department, Memorial University, St. John's, Newfoundland, Canada.
Khan
S
S
O'Brien
P J
PJ
Payne
J F
JF
Rahimtula
A D
AD
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D002642:Chick Embryo; D004790:Enzyme Induction / Q000187:drug effects; D006571:Heterocyclic Compounds / Q000633:toxicity*; D006838:Hydrocarbons / Q000633:toxicity*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D010578:Petroleum / Q000633:toxicity*
D006571:Heterocyclic Compounds; D006838:Hydrocarbons; D010578:Petroleum; D001189:Aryl Hydrocarbon Hydroxylases