Properties of a new crystal form of the complex of concanavalin A with methyl alpha-D-glucopyranoside.
The complex of concanavalin A with methyl alpha-D-glucopyranoside crystallizes as regular rhombic dodecahedra containing 35% protein by weight. The crystal is of space group I23 with a = 167.8 A (1 A = 0.1 nm) and contains one concanavalin A dimer per asymmetric unit. It diffracts to a resolution of 1.9 A and is suitable for crystallographic investigation of the structure of the saccharide-binding site.
Yariv
J
J
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Kalb
A J
AJ
Papiz
M Z
MZ
Helliwell
J R
JR
Andrews
S J
SJ
Habash
J
J
D001665:Binding Sites; D003208:Concanavalin A / Q000378:metabolism*; D003460:Crystallization; D003461:Crystallography; D046911:Macromolecular Substances; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D008757:Methylglucosides; D008759:Methylglycosides; D003208:Concanavalin A