Deletion and duplication of DNA sequences is associated with the embryonic lethal phenotype of the t9 complementation group of the mouse t complex.
We have analyzed the genomic structure of three mouse t haplotypes of the t9 complementation group. Each of these t haplotypes, tw18, t4, and tks1, is known to have resulted from a rare recombination event between a complete t haplotype and a wild-type chromosome. Using molecular probes that identify sequences in the distal portion of the t complex, we have shown that each of these t haplotypes contains a similar (perhaps identical) deletion of one group of t complex sequences, and duplication of another group. These data suggest that the recombination events that produced these three t haplotypes involved similar unequal crossovers within the distal inversion. The deletion and duplication of genetic material associated with all members of the t9 complementation group tested provides a molecular explanation for the recessive lethal mutation associated with these t haplotypes.
Búcan
M
M
European Molecular Biology Laboratory, Heidelberg, Germany.
Herrmann
B G
BG
Frischauf
A M
AM
Bautch
V L
VL
Bode
V
V
Silver
L M
LM
Martin
G R
GR
Lehrach
H
H
D000818:Animals; D002872:Chromosome Deletion; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004261:DNA Replication; D005260:Female; D005313:Fetal Death; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D006239:Haplotypes; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D011247:Pregnancy
D004247:DNA