Corneal test as a reliable method for detection of the delayed-type hypersensitivity reaction in mice.
Antigen solution could be injected into the cornea of sensitized mice using a fine needle and a stereoscopic dissecting microscope. The resulting corneal reaction was shown to be a reliable method in the detection and estimation of delayed-type hypersensitivity in mice that had been immunized with a water-in-oil emulsion containing an ovalbumin and a cell wall adjuvant. Unlike the delayed skin reaction in the ear lobe, this corneal reaction was not affected by a coexisting Arthus reaction.
Morisaki
I
I
Department of Microbiology and Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
Shimono
T
T
Kohno
N
N
Stewart-Tull
D E
DE
Kotani
S
S
Kitamura
H
H
D000818:Animals; D000917:Antibody Formation; D003315:Cornea / Q000276:immunology* / Q000473:pathology; D006968:Hypersensitivity, Delayed / Q000175:diagnosis* / Q000276:immunology; D007114:Immunization; D007159:Immunologic Tests; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D010047:Ovalbumin / Q000276:immunology*; D011237:Predictive Value of Tests
D010047:Ovalbumin