Cortically induced masticatory rhythm in masseter motoneurons after blocking inhibition by strychnine and tetanus toxin.
In the first series of experiments, we studied whether or not strychnine (STR)-resistant inhibition of masseter motoneurons (MASS . MNS) was involved in their rhythmical inhibition that occurs during masticatory activity, induced by repetitive stimulation of the cortical masticatory area (CMA) in the cat. After systemic STR injection, repetitive CMA stimulation induced rhythmically alternating activity in the masseteric and anterior digastric nerves with a shorter cycle time than before STR-administration. The short-latency IPSPS in the MASS . MNS evoked by single shocks applied to the CMA were abolished. In contrast, repetitive CMA stimulation still induced a rhythmical alternation of EPSPS and IPSPS in the MASS . MNS, although the IPSPS were significantly reduced in amplitude. In the second series, we attempted to abolish the STR-resistant component of the rhythmical IPSP with tetanus toxin (TT). This was injected into one superficial masseter muscle of the guinea pig. In the majority of animals, repetitive CMA stimulation induced a tonic EMG superimposed by rhythmical bursts in the TT-intoxicated masseter muscle. Repetitive CMA stimulation induced a rhythmical sequence of EPSPS and superimposed spikes in the MASS . MNS innervating the TT-intoxicated masseter muscle in paralyzed guinea pigs. It was concluded that: (1) the cortically-evoked short-latency inhibition of MASS . MNS is STR-sensitive, as is part of the rhythmical inhibition during CMA-induced mastication; and (2) rhythmical inhibition is not essential for the central generation of the rhythmical activity in the MASS . MNS.
Enomoto
S
S
Department of Physiology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Katakura
N
N
Sunada
T
T
Katayama
T
T
Hirose
Y
Y
Ishiwata
Y
Y
Nakamura
Y
Y
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002415:Cats; D002540:Cerebral Cortex / Q000502:physiology*; D004576:Electromyography; D008406:Masseter Muscle / Q000294:innervation* / Q000502:physiology; D008410:Masticatory Muscles / Q000294:innervation*; D008564:Membrane Potentials / Q000187:drug effects; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D009433:Neural Inhibition / Q000187:drug effects*; D013331:Strychnine / Q000494:pharmacology*; D013744:Tetanus Toxin / Q000494:pharmacology*
D013744:Tetanus Toxin; D013331:Strychnine