title: Transport of immune complexes from the subcapsular sinus into the lymph node follicles of the rat.
abstract: To study the mode of transport of immune complexes from the subcapsular sinus into the follicles of draining popliteal lymph nodes, horse radish peroxidase (HRP)-anti HRP was injected in rat footpads. Within six minutes, complexes were already present in the subcapsular sinus freely or attached to the plasma membrane of different types of cell including cells forming the stroma. A few minutes later, complexes were also seen in the deeper part of the outer cortex, and after two hours they had reached the periphery of the follicles. They were always seen scattered between lymphoid and non-lymphoid cells. After one day, complexes were present on well-developed follicular dendritic cells. After injection of HRP, no localization of this antigen was observed in the deeper part of the outer cortex including the follicles. These results strongly suggest that HRP-anti HRP complexes are passively transported through the outer cortex into the follicles where they are trapped and retained by follicular dendritic cells.
authors: Department of Electron Microscopy, Medical Faculty, Free University, Amsterdam, The Netherlands.
authors: E W
authors: EW
authors: Kamperdijk
authors: C D
authors: CD
authors: Dijkstra
authors: E A
authors: EA
authors: Döpp
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D006735:Horseradish Peroxidase; D007267:Injections; D008198:Lymph Nodes / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000936:Antigen-Antibody Complex; D006735:Horseradish Peroxidase