title: Fetal frontal cortex transplanted to injured motor/sensory cortex of adult rats. I. NADPH-diaphorase neurons.
abstract: Fetal frontal cortex from 18-d-old embryonic rat brain was transplanted into cavities of juvenile host motor/sensory cortex. Two to seven months later, sections were reacted for NADPH-diaphorase (NADPH-d) enzyme histochemistry. NADPH-d-positive neurons survived in 11 of 13 grafts. All but one of the transplants had reduced numbers of these neurons, although in 3 transplants the reductions were moderate and not statistically significant. The distribution and morphology of NADPH-d neurons within most grafts was comparable to that of NADPH-d neurons in normal host cortex. At the margin of the 2 transplants with no NADPH-d neuronal perikarya, NADPH-d fibers crossed from host to transplant as far as a millimeter into the transplant, and on rare occasions, the host neurons that gave rise to these fibers were identified. This suggests that host-transplant interactions are possible. One transplant had an abnormally large number of NADPH-d-positive neurons and fibers, possibly due to selective survival of these neurons. The data reported here for NADPH-d in cortical transplants may also apply to neuropeptide Y (NPY), since nearly all neocortical NPY neurons also contain NADPH-d.
authors: Department of Neurology, University of California, San Francisco.
authors: M F
authors: MF
authors: Gonzalez
authors: F R
authors: FR
authors: Sharp
mesh_terms: D000818:Animals; D005333:Fetus; D005625:Frontal Lobe / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000637:transplantation*; D006085:Graft Survival; D008297:Male; D009044:Motor Cortex / Q000502:physiology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009412:Nerve Fibers / Q000201:enzymology / Q000502:physiology; D009474:Neurons / Q000201:enzymology*; D051381:Rats; D013003:Somatosensory Cortex / Q000502:physiology*
chemical_list: D009247:NADH, NADPH Oxidoreductases; D009252:NADPH Dehydrogenase