An early intermediate of refolding alpha-lactalbumin forms within 20 ms.
The kinetics of alpha-lactalbumin refolding were studied by the stopped-flow method with the registration of CD and intrinsic fluorescence at several wavelengths. It was shown that the early kinetic intermediate forms during the dead-time of the experiment (20 ms). This intermediate has a considerable amount of secondary structure and unpolar clusters in its molecular structure but has no rigid tertiary structure.
Gilmanshin
R I
RI
Institute of Protein Research, Academy of Sciences of the USSR, Moscow Region.
Ptitsyn
O B
OB
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D007700:Kinetics; D007768:Lactalbumin; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D007768:Lactalbumin