Structure of two new aminophospholipids from Methanobacterium thermoautotrophicum.
The methanogenic bacterium Methanobacterium thermoautotrophicum (A.T.C.C. 29183) was shown to contain two new aminophospholipids. These are 2-aminoethyl phosphate ester of diphytanylglycerol diether and a sugar containing bisdiphytanyldiglycerol tetraether. The two aminophospholipids were stable to acid methanolysis except for the sugar on the bisdiphytanyldiglycerol tetraether. Strong acid (6 M-HCl) hydrolysed the alkyl ether and aminophosphate ester bonds. The structure of the phosphate linkage was demonstrated by 31P n.m.r., and the 2-ethanolamine structure was elucidated by 1H- and 13C-n.m.r. spectroscopy and by fast-atom-bombardment m.s.
Kramer
J K
JK
Animal Research Centre, Agriculture Canada, Ottawa, Ontario.
Sauer
F D
FD
Blackwell
B A
BA
D002855:Chromatography, Thin Layer; D019605:Euryarchaeota / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000302:isolation & purification*
D010743:Phospholipids