Adenine specific DNA chemical sequencing reaction.
Reaction of DNA with K2PdCl4 at pH 2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCl4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.
Iverson
B L
BL
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.
Dervan
P B
PB
D000225:Adenine; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D010165:Palladium; D010880:Piperidines; D010957:Plasmids
D004269:DNA, Bacterial; D010880:Piperidines; C030174:tetrachloropalladium; D010165:Palladium; C032727:piperidine; D004247:DNA; D000225:Adenine