A differential pulse polarographic serum protein assay.
Human serum and albumin show a differential pulse polarographic band near -0.6 V. For sufficiently dilute solutions (less than 7 micrograms/ml) at pH 7.0, the polarographic response is linearly related to the solution concentration. At pH 12.6 a near-linear response to 70 micrograms/ml is obtained, but sample equilibration time is slow. The polarographic assay for total serum protein using bovine serum albumin (BSA) to generate a standard curve gave a correlation coefficient of 0.89. The cellular constituents of whole blood do not interfere. The correlation with BSA does not hold for protease-digested serum or albumin.
Hertl
W
W
Research and Development Division, Corning, New York 14831.
D000327:Adsorption; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D005898:Glass; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008832:Microchemistry; D010969:Plastics; D011048:Polarography; D012709:Serum Albumin / Q000032:analysis; D012996:Solutions
D001798:Blood Proteins; D010969:Plastics; D012709:Serum Albumin; D012996:Solutions