Preserved respiratory and phagocytic functions of phagocytes exposed to flat sheet plasmapheresis equipment.
Monocytes and polymorphonuclear leukocytes (PMN) were tested for functional integrity after exposure to flat sheet plasmapheresis equipment. Purified PMN were tested for chemiluminescence activity in response to a variety of triggers of the respiratory burst. Monocytes were assessed for their capacity to ingest erythrocytes sensitized with varying amounts of IgG antibodies. Both assays were demonstrated to be sensitive hallmarks of functional modulation. However, no functional differences were noted between phagocytes from blood collected prior to pheresis and those exposed to flat sheet plasmapheresis cylinders. These data suggest that plasmapheresis with the Autopheresis C system does not influence the respiratory and phagocytic function of phagocytes returned back to the donor and implies that contact of phagocytes with artificial surfaces and/or their exposure to surface-activated plasma factors generated in the collection cylinder are minimal when using this plasmapheresis method.
Jungi
T W
TW
Institute for Clinical and Experimental Cancer Research, Berne, Switzerland.
Aeschbacher
B
B
Nydegger
U E
UE
D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology; D010586:Phagocytes / Q000276:immunology*; D010587:Phagocytosis; D010956:Plasmapheresis / Q000295:instrumentation*; D011961:Receptors, Fc / Q000276:immunology
D011961:Receptors, Fc