The microphotometric determination of the variability of oxygen saturation of erythrocytes lying within rouleaux.
It was found that by using nonlinear multicomponent analysis with the spectra of oxygenated and deoxygenated haemoglobin as basic spectra the mean O2 saturation of 2 to 3 red cells lying in a rouleau could be determined with an accuracy of 0.5 - 1.0%. At the same PO2 and PCO2 distinct differences in O2 saturation were found in different red cells; for 50% of the saturation values the differences were in the range +/- 1.5%, for 30% of the values, the differences were in the range of +/- 3% and 15% lay in the range of +/- 4.5%. Only 5% of saturation differences were larger than 4.6%. These differences are so large that they have to be considered in calculations of the PO2 from O2 saturation measurements.
Lubbers
D W
DW
Max-Planck-Institut fur Systemphysiologie, Dortmund, F.R.G.
Grisar
H
H
Gayeski
T E
TE
D002448:Cell Adhesion; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D010100:Oxygen / Q000097:blood*; D012016:Reference Values; D013053:Spectrophotometry
D006454:Hemoglobins; D010100:Oxygen