Neodymium:YAG laser interaction with Alcon IOGEL hydrogel intraocular lenses: an in vitro toxicity assay.
The objective of this study was to determine the potential toxicity generated by the interaction of the Nd:YAG laser and Alcon IOGEL intraocular hydrogel lens material. The IOGEL lens is composed of poly 2-hydroxyethylmethacrylate, containing 38% water, previously shown to be highly biocompatible in a wide range of tissue culture and implantation experiments. In this study, intraocular lenses (IOLs) immersed in serum-free cell culture medium were purposely exposed to exaggerated doses of laser energy to cause extensive damage. An IOLAB polymethylmethacrylate (PMMA) lens served as a control lens material. The resultant solutions were assayed for cytotoxicity in a bioassay system using fourth passage human corneal endothelial cells. No cytotoxicity was seen in the bioassay for the IOGEL hydrogel IOLs or the PMMA control IOL at any laser range/dosage tested over a 72-hour incubation period. Hydrogel lenses exhibited decreasing yellowing with decreasing energy levels, and no lens discoloration was apparent at the lowest level of irradiation, 5 mJ/50 laser bursts; the PMMA control lens exhibited moderate yellowing at 15 mJ/50 bursts. Lens marking was moderate for all IOGEL IOLs; the PMMA lens marking was severe at the power level tested.
Skelnik
D L
DL
Department of Ophthalmology, University of Minnesota, Minneapolis 55455.
Lindstrom
R L
RL
Allarakhia
L
L
Tamulinas
C
C
Lorenzetti
O J
OJ
D002387:Cataract Extraction; D002470:Cell Survival; D002478:Cells, Cultured; D004728:Endothelium, Corneal / Q000473:pathology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D053685:Laser Therapy; D007910:Lenses, Intraocular; D008768:Methylmethacrylates; D011092:Polyethylene Glycols; D011183:Postoperative Complications / Q000473:pathology*; D011474:Prosthesis Design; D011475:Prosthesis Failure
D008768:Methylmethacrylates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols