HLA expression on human germinal cells.
Ejaculated human seminal plasma cells obtained from 46 healthy men and 48 infertile patients were tested for expression of HLA class I and II antigens by complement-mediated cytotoxicity, cell-binding radioimmunoassay (CB-RIA), and an ELISA involving monoclonal antibodies (mAbs). In a group of healthy men tested for HLA expression on human spermatozoa, 4 of 46 were positive for class I and II HLA antigens. However, the results were negative in a second examination of the same donors, possibly on account of alterations with time in the expression of various subpopulations of cells which are antigen positive or antigen negative. In a group of infertile patients, we found positive expression of HLA class I and II antigens in 7 of 48 men. The samples contained immature and mature sperm but no contaminating leucocytes or epithelial cells.
Kurpisz
M
M
Institute of Human Genetics, Polish Academy of Science, Poznan.
Fernandez
N
N
Witt
M
M
Kowalik
I
I
Szymczynski
G A
GA
Festenstein
H
H
D000911:Antibodies, Monoclonal; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens / Q000032:analysis*; D006681:HLA-D Antigens / Q000032:analysis*; D006801:Humans; D007248:Infertility, Male / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D013094:Spermatozoa / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006681:HLA-D Antigens