Freeze-fracture electron microscopic observations on the effects of sulphydryl group reagents on human erythrocyte membranes.
Freeze-fracture electron microscopy of human red blood cells at pH 7.4 and 5.5 reveals the presence of membrane elevations (50-100 nm diameter). These are also observed after incubation of the erythrocytes with N-ethylmaleimide but not after incubation with p-chloromercuribenzene sulphonate. Neither of the sulphydryl-group reagents affects the distribution or size of intramembrane particles. The findings are discussed in the light of the effects of mercurials on erythrocyte membrane proteins.
Benga
G
G
Department of Cell Biology, Faculty of Medicine, Cluj-Napoca, Roumania.
Brain
A
A
Pop
V I
VI
Hodarnau
A
A
Wrigglesworth
J M
JM
D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000648:ultrastructure; D005614:Freeze Fracturing / Q000379:methods; D006801:Humans; D008854:Microscopy, Electron; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D013439:Sulfhydryl Reagents