A mutational analysis of the bacteriophage P1 recombinase Cre.
Bacteriophage P1 encodes a 38,600 Mr site-specific recombinase, Cre, that is responsible for reciprocal recombination between sites on the P1 DNA called loxP. Using in vitro mutagenesis 67 cre mutants representing a total of 37 unique changes have been characterized. The mutations result in a wide variety of phenotypes as judged by the varying ability of each mutant Cre protein to excise a lacZ gene located between two loxP sites in vivo. Although the mutations are found throughout the entire cre gene, almost half are located near the carboxyl terminus of the protein, suggesting a region critical for recombinase function. DNA binding assays using partially purified mutant proteins indicate that mutations in two widely separated regions of the protein each result in loss of heparin-resistant complexes between Cre and a loxP site. These results suggest that Cre may contain two separate domains, both of which are involved in binding to loxP.
Wierzbicki
A
A
Central Research and Development Department, E.I. du Pont de Nemours and Company, Inc., Wilmington, DE 19898.
Kendall
M
M
Abremski
K
K
Hoess
R
R
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004274:DNA, Recombinant; D004279:DNA, Viral; D005814:Genes, Viral; D019426:Integrases; D009154:Mutation; D014764:Viral Proteins
D004274:DNA, Recombinant; D004279:DNA, Viral; D014764:Viral Proteins; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; D019426:Integrases