title: A water-soluble, monitorable peptide and protein crosslinking agent.
abstract: A novel, freely water-soluble, heterobifunctional crosslinking reagent, N-maleimido-6-aminocaproyl ester of 1-hydroxy-2-nitro-4-benzenesulfonic acid (mal-sac-HNSA), was synthesized and used for conjugation of sulfhydryl (cysteine)-containing peptides to carrier proteins. Reaction with amino groups releases the dianion phenolate, HNSA, which allows convenient spectrophotometric quantitation of the reaction in progress. Since mal-sac-HNSA is completely water soluble, its concentration can be adjusted to maximize the rate of amine reaction and to minimize hydrolysis. Conjugates of peptides to appropriate carriers have elicited peptide-specific antibody and did not elicit detectable antibody specific to the crosslink.
authors: Cetus Corporation, Emeryville, California 94608.
authors: L
authors: L
authors: Aldwin
authors: D E
authors: DE
authors: Nitecki
mesh_terms: D001557:Benzenesulfonates / Q000138:chemical synthesis; D003432:Cross-Linking Reagents / Q000138:chemical synthesis; D008301:Maleimides / Q000138:chemical synthesis; D010455:Peptides; D011506:Proteins; D012995:Solubility; D014867:Water
chemical_list: D001557:Benzenesulfonates; D003432:Cross-Linking Reagents; D008301:Maleimides; D010455:Peptides; D011506:Proteins; D014867:Water; C053984:N-maleimido-6-aminocaproyl 1-hydroxy-2-nitro-4-benzenesulfonic acid ester