Splicing of plant pre-mRNAs in animal systems and vice versa.
To investigate similarities and differences of pre-mRNA splicing between higher plants and animals, we tested whether pre-mRNAs of higher plants can be accurately spliced in animal systems, and whether an animal pre-mRNA can be spliced in plant cells. Pre-mRNAs of the maize bronze locus and oat phytochrome type 3 are accurately spliced with moderate efficiency in a human (HeLa cell) nuclear extract. The first intervening sequence (IVS1) of bean phaseolin pre-mRNA is not excised in the human nuclear extract, but is removed (although at low efficiency) in intact monkey cells. However, the IVS1 of human alpha-globin pre-mRNA is not removed in tobacco cells. Our data suggest that the mechanisms of pre-mRNA splicing are similar, but not identical, in plants and animals.
van Santen
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VL
Laboratory of Genetics, University of Wisconsin, Madison 53706.
Spritz
R A
RA
D000818:Animals; D005914:Globins / Q000235:genetics; D006801:Humans; D007438:Introns; D010834:Phytochrome / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D010944:Plants / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing; D014158:Transcription, Genetic
D010940:Plant Proteins; D012322:RNA Precursors; C048826:phaseolin protein, Phaseolus vulgaris; D010834:Phytochrome; D005914:Globins