Preliminary X-ray data for a D-amino acid amino-transferase from a novel thermophilic Bacillus.
Crystals of the D-amino acid aminotransferase (D-ATA) from a novel thermophilic Bacillus species (Escherichia coli pICT113 cloned gene product) have been examined by X-ray analysis. The crystals grow as hexagonal prisms, with the symmetry of space group P61 or P65 (indistinguishable crystallographically). The cell dimensions are a = b = 135 A, c = 53 A, alpha = beta = 90 degrees, and gamma = 120 degrees. The unit cell has a volume of 850,000 A3 with six asymmetric units per unit cell. There is one dimer of molecular weight 62,000 per asymmetric unit, and the crystals diffract to 2.7 A.
Stoddard
B
B
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 12139.
Howell
L
L
Asano
S
S
Soda
K
K
Tanizawa
K
K
Ringe
D
D
Petsko
G A
GA
D001407:Bacillus / Q000201:enzymology*; D003461:Crystallography; D000637:Transaminases
D000637:Transaminases