An enzyme-linked immunoassay for lipoprotein lipase.
Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.
Goers
J W
JW
Department of Chemistry, California Polytechnic State University, San Luis Obispo 93407.
Pedersen
M E
ME
Kern
P A
PA
Ong
J
J
Schotz
M C
MC
D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000032:analysis; D007668:Kidney / Q000201:enzymology; D008071:Lipoprotein Lipase / Q000032:analysis*; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008715:Methionine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D008715:Methionine; D008071:Lipoprotein Lipase