Characterization of concanavalin A induced peritoneal exudate cells in infant mice.
Con A induced peritoneal exudate (PE) cells were characterized in 9-12 days old infant mice. It was observed that 100-200 micrograms of Con A induces two peaks viz. first at 48 hrs post inoculation (PI) and second on 8-10 days PI. Majority of the induced cell population was adherent macrophages (75-80%) and non adherent population was 20-25%. During 2nd peak of induction non adherent population increased upto 40%. The macrophage population was heterogenous. However, majority of them were 8-12 micron in size. The cells showed strong non specific esterase and acid phosphatase activities. Subsequently, there was vesicle formation in this population. Con A also induced vesiculation in PE cells markedly when compared with normal unstimulated mice.
Kelkar
S D
SD
Department of Virology, National Institute of Virology, Pune, India.
Gogate
S S
SS
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004950:Esterases / Q000032:analysis; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology* / Q000276:immunology
D003208:Concanavalin A; D004950:Esterases; D000135:Acid Phosphatase