title: Experimental demonstration of the immunogenicity of silicone-protein complexes.
abstract: Although silicones, as a class, are nontoxic in animal and tissue studies, implanted silicone prostheses and medical devices are associated with various local and systemic host inflammatory reactions. They also have been associated with a form of autoimmune disease. To test the hypothesis that silicones may evoke an immunologically mediated inflammatory reaction, 10 guinea pigs were stimulated for 1 month with intraperitoneal injections of sterile medical-grade silicone oil admixed with homologous serum and complete Freund's adjuvant. Ten controls were stimulated with saline. Four additional animals were passively sensitized with splenic homogenates from four sensitized animals. Intradermal antigenic challenges consisted of silicone-homologous serum, pure silicone, saline-homologous serum, and purified protein derivative. Cutaneous reaction patterns were graded grossly and microscopically. Silicone-serum and purified protein derivative antigens evoked three to four times greater palpable lesions in all 10 actively and all 4 passively sensitized animals at approximately 24 h compared to controls. Biopsies showed a moderate to marked lymphocytic infiltrate. Control sites and naive animals showed only edema at the challenge sites. The data suggest that silicone-protein complexes are potentially immunogenic.
authors: Department of Pathology, University of California at Los Angeles 90024.
authors: N
authors: N
authors: Kossovsky
authors: J P
authors: JP
authors: Heggers
authors: M C
authors: MC
authors: Robson
mesh_terms: D000818:Animals; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000473:pathology; D007116:Immunization, Passive; D007249:Inflammation / Q000209:etiology*; D007274:Injections, Intraperitoneal; D007428:Intradermal Tests; D012826:Silicone Elastomers / Q000276:immunology*; D013997:Time Factors
chemical_list: D012826:Silicone Elastomers