title: Comparison of culture media for detecting methicillin resistance in Staphylococcus aureus and coagulase negative staphylococci.
abstract: The detection of methicillin resistance was examined in 51 strains of Staphylococcus aureus and 135 strains of coagulase negative staphylococci using Isosensitest, Diagnostic Sensitivity Test (DST), Mueller-Hinton (MH), Columbia, and Sensitest agars. MH agar with 5% added sodium chloride incubated at 35 degrees C was the most effective in detecting resistance in S aureus, and Columbia agar with 5% added sodium chloride incubated at 35 degrees C was most effective for coagulase negative staphylococci. For clinical purposes, a provisional report of sensitivity for S aureus could be issued after 18 hours; with coagulase negative staphylococci, only resistant strains could be reported at this time. For definitive results cultures must be examined after 40 hours of incubation.
authors: Public Health Laboratory, John Radcliffe Hospital, Oxford.
authors: L M
authors: LM
authors: Milne
authors: G D
authors: GD
authors: Curtis
authors: M
authors: M
authors: Crow
authors: W A
authors: WA
authors: Kraak
authors: J B
authors: JB
authors: Selkon
mesh_terms: D003030:Coagulase / Q000378:metabolism; D003470:Culture Media; D008712:Methicillin / Q000494:pharmacology*; D010403:Penicillin Resistance; D012965:Sodium Chloride; D013210:Staphylococcus / Q000201:enzymology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013997:Time Factors
chemical_list: D003030:Coagulase; D003470:Culture Media; D012965:Sodium Chloride; D008712:Methicillin