title: Effect of chronic alcohol consumption on the pregnant mare serum gonadotropin-induced luteinizing hormone surge.
abstract: The studies reported in this paper were undertaken to investigate the effect of chronic (10 day) alcohol consumption on female pituitary-gonadal function. The immature female rat model treated with pregnant mare serum gonadotropin (PMSG) was used since it results in a highly reproducible luteinizing hormone (LH) surge and ovulation. Twenty-day-old female rats were placed on diets that were either (1) unrestricted (ad libitum); (2) contained 5% ethanol in a liquid diet, or (3) isocalorically pair-fed with the liquid diet to the ethanol group. After 10 days on their respective diets, the groups were subdivided and given either 8 IU of PMSG in 0.1 ml saline or 0.1 ml saline s.c. between 10.00 and 11.00 h. The animals were sacrificed by decapitation at 24, 48, 52, 54, 56, 58, 60 and 62 h after injection. Trunk blood was obtained for serum measurements of LH. The uteri were weighed and prepared for the histological study. In all dietary groups, serum LH levels were significantly higher in the PMSG-treated animals when compared to the saline controls at all time intervals with the exception of the alcohol 58-hour group. In the ad libitum animals, plasma LH concentrations were highest at 52 h following hormone administration. The serum LH concentrations were highest at 56 h after hormone administration in the pair-fed group and were significantly less than the ad libitum group at 52, 54, 56, and 58 h after PMSG stimulation. No significant plasma LH surge was observed in the alcohol group and the LH concentrations were significantly less than the pair-fed rats at 56 and 58 h after PMSG treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Physiology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, N.C.
authors: D K
authors: DK
authors: Sundberg
authors: W J
authors: WJ
authors: Bo
authors: J
authors: J
authors: Reilly
mesh_terms: D000437:Alcoholism / Q000097:blood*; D000818:Animals; D002149:Energy Intake; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood*; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011863:Radioimmunoassay; D051381:Rats; D014599:Uterus / Q000187:drug effects / Q000473:pathology
chemical_list: D006064:Gonadotropins, Equine; D007986:Luteinizing Hormone