Effect of age on behavioral and enzymatic changes during thiamin deficiency.
Open field behavior and whole brain enzymatic activities were determined during thiamin deficiency in two strains of young, as well as in aged mice. In young CD-1 mice, thiamin deficiency reduced total distance traveled and vertical movements after 7 days and the decline was more than 50% by day 9. The behavioral deficit was highly correlated to decreases in 2-oxoglutarate dehydrogenase activity (KGDH). The open field behavior of Balb/c mice was about 40% less than in CD-1 mice and responded in a qualitatively different manner to thiamin deficiency. The activity of the Balb/c mice increased and then decreased with thiamin deficiency. The activity of 3 month old mice peaked on day 6 (126% of initial score), whereas 10 and 30 month mice showed a much greater increase (about 175% of initial scores), but on day 7. Although the activity of the thiamin dependent enzyme transketolase (TK) was affected similarly at all ages, the activity of KGDH in the aged brain was more sensitive to thiamin deficiency than in the young; KGDH activity declined 41%, 57% or 74% at 3, 10, or 30 months, respectively. Thus, the current mouse model is an attractive one to study the interaction of thiamin deficiency with aging.
Freeman
G B
GB
Cornell University Medical College, Burke Rehabilitation Center, White Plains, NY 10605.
Nielsen
P E
PE
Gibson
G E
GE
D000375:Aging / Q000378:metabolism* / Q000523:psychology; D000818:Animals; D001835:Body Weight; D001921:Brain / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000502:physiology*; D011745:Pyrithiamine; D013832:Thiamine Deficiency / Q000201:enzymology / Q000503:physiopathology*; D014174:Transketolase / Q000378:metabolism
D011745:Pyrithiamine; D007655:Ketoglutarate Dehydrogenase Complex; D014174:Transketolase; D002795:Choline O-Acetyltransferase