title: Antibodies against glycosphingolipids in sera of patients with idiopathic thrombocytopenic purpura.
abstract: Evidence is presented for the existence of antibodies against platelet glycosphingolipids in sera of patients with chronic idiopathic thrombocytopenic purpura and antiplatelet antibodies. Increased binding of IgG/IgM to neutral glycosphingolipids or gangliosides extracted from donor platelets was measured by an ELISA in 17 sera of 30 patients. Thirteen sera, five with anticardiolipin antibodies, demonstrated an increased binding to sulphatides confirmed by immuno HPTLC. Four sera showed reactivity with the platelet minor gangliosides established by immuno-HPTLC.
authors: Department of Haematology, University Hospital Rotterdam-Dijkzigt, The Netherlands.
authors: H H
authors: HH
authors: van Vliet
authors: M C
authors: MC
authors: Kappers-Klunne
authors: J W
authors: JW
authors: van der Hel
authors: J
authors: J
authors: Abels
mesh_terms: D000906:Antibodies / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis; D001792:Blood Platelets / Q000276:immunology*; D002308:Cardiolipins / Q000276:immunology; D005732:Gangliosides / Q000276:immunology; D006028:Glycosphingolipids / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D011696:Purpura, Thrombocytopenic / Q000276:immunology*; D013433:Sulfoglycosphingolipids / Q000276:immunology
chemical_list: D000906:Antibodies; D001323:Autoantibodies; D002308:Cardiolipins; D005732:Gangliosides; D006028:Glycosphingolipids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013433:Sulfoglycosphingolipids