Procoagulant activity of platelet arachidonic acid metabolites.
1 Arachidonate metabolites have been extracted from indomethacin-treated human platelets after incubation with arachidonic acid. 2 After separation from platelet phospholipids, the extracts promoted the generation of large amounts of thrombin in normal plasma, but not in plasma devoid of lipoproteins. 3 The procoagulant activity was associated with a minor component of the mixture, which was active at concentrations below 10 micrograms ml-1. 4 The activity was similar to that of autoxidised arachidonic acid previously described. 5 Platelet arachidonic acid metabolites could play a role in the coagulation system.
Barrowcliffe
T W
TW
Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire.
Edwards
S J
SJ
Gray
E
E
Kerry
P J
PJ
D001095:Arachidonic Acids / Q000097:blood / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D001792:Blood Platelets / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D006801:Humans; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000494:pharmacology; D010084:Oxidation-Reduction; D010314:Partial Thromboplastin Time; D010743:Phospholipids / Q000097:blood
D001095:Arachidonic Acids; D008054:Lipid Peroxides; D010743:Phospholipids