Kinetic pulse-labeling study of Fusarium culmorum. Biosynthetic intermediates and dead-end metabolites.
A kinetic pulse-labeling method was utilized in Fusarium culmorum to detect plausible biosynthetic intermediates and differentiate them from dead-end metabolites. The ultimate test to demonstrate a precursor relies on feeding experiments. We now report the detection of four new metabolites, one of them (compound 1) behaves as a dead-end metabolite, whereas compounds 2, 3, and 4 seem to be putative intermediates: they metabolize with time just when 3-acetyldeoxynivalenol (3-ADN) and/or sambucinol (SOL) start to be produced. Feeding experiments confirmed these results: compound 1 is not converted to 3-ADN or SOL, and compounds 2-4 are precursors to 3-ADN. In addition 3 is a precursor to SOL.
Zamir
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LO
Université du Québec, Institut Armand-Frappier, Laval-des-Rapides, Canada.
Devor
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KA
D005670:Fusarium / Q000378:metabolism*; D007700:Kinetics; D008798:Mevalonic Acid / Q000378:metabolism; D008954:Models, Biological; D014255:Trichothecenes / Q000096:biosynthesis
D014255:Trichothecenes; C043247:3-acetyldeoxynivalenol; C054183:sambucinol; D008798:Mevalonic Acid