Selective inhibition of protein synthesis by hypertonic medium in Marituba (Bunyaviridae) virus-infected L-A9 cells.
Elevation of the NaCl concentration in the growth medium of L-A9 cells caused an inhibition of the protein synthesis accompanied by a complete breakdown of polyribosomes. However, a complete recovery of the rate of protein synthesis was observed when isotonicity was restored. In Marituba virus infected cells, protein synthesis became resistant to hypertonic treatment. Under hypertonic conditions cellular protein synthesis was selectively suppressed and an enhancement of virus proteins was observed. Analysis of the virus specific proteins by polyacrylamide gel electrophoresis revealed that the synthesis of G1 was unalterable, and N was stimulated.
Frugulhetti
I C
IC
Instituto de Biofísica Carlos Chagas Filho, UFRJ CCS, Cidade Universitária, Rio de Janeiro, Brazil.
Tavares
C C
CC
Rebello
M A
MA
D000818:Animals; D002043:Bunyaviridae / Q000378:metabolism*; D002460:Cell Line; D003470:Culture Media; D006982:Hypertonic Solutions / Q000494:pharmacology*; D051379:Mice; D014176:Protein Biosynthesis; D012965:Sodium Chloride / Q000494:pharmacology; D014764:Viral Proteins / Q000096:biosynthesis
D003470:Culture Media; D006982:Hypertonic Solutions; D014764:Viral Proteins; D012965:Sodium Chloride