title: Developmental studies of Hanford miniature swine exposed to 60-Hz electric fields.
abstract: Evaluations of reproductive and developmental toxicology, including teratology, were included as part of a broad screening study in Hanford Miniature swine (HMS) to detect effects of exposure to electric fields. One group (E) was exposed to a uniform, vertical, 60-Hz, 30-kV/m electric field for 20 h/day, 7 days/week; sham-exposed (SE) swine were housed in a separate, environmentally equivalent building. The first generation (F0) gilts were bred after 4 months of study; some were killed for teratologic assays at 100 days of gestation (dg), and the others produced an F1 generation of offspring. The pooled incidence of terata in these litters (teratologic assays and live births) was similar in the E and SE groups. The F0 females, which produced the F1 generation, were bred again after 18 months of exposure and were killed at 100 dg. Malformation incidence in E litters (75%) was significantly greater than in SE litters (29%). No consistent differences in litter size, fetal mass, or mass of fetal organs were detected. The F1 gilts were bred at 18 months of age; defective offspring were found in significantly more of the E litters (71%) than in SE litters (33%). These F1 females were bred again 10 months later and teratologic assays were performed on their second litters at 100 dg. The percentage of litters with malformed fetuses was essentially identical in the E and SE groups (70% and 73%, respectively). There appears to be an association between chronic exposure to a strong electric field and developmental effects in swine, although the change in incidence of malformations between generations and between the first and second breedings makes it impossible to conclude unequivocally that there is a cause-and-effect relation.
authors: Biology and Chemistry Department, Pacific Northwest Laboratories, Richland, Washington 99352.
authors: M R
authors: MR
authors: Sikov
authors: D N
authors: DN
authors: Rommereim
authors: J L
authors: JL
authors: Beamer
authors: R L
authors: RL
authors: Buschbom
authors: W T
authors: WT
authors: Kaune
authors: R D
authors: RD
authors: Phillips
mesh_terms: D000818:Animals; D001724:Birth Weight; D000013:Congenital Abnormalities / Q000209:etiology*; D004574:Electromagnetic Fields; D055590:Electromagnetic Phenomena; D005260:Female; D005298:Fertility; D006128:Growth / Q000528:radiation effects*; D008098:Litter Size; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000528:radiation effects*; D012098:Reproduction / Q000528:radiation effects; D013552:Swine; D013723:Teratogens
chemical_list: D013723:Teratogens