Determination of total cholesterol in serum by flow injection analysis with immobilized enzymes.
Several photometric and fluorimetric methods are proposed for the determination of cholesterol by use of enzymes immobilized on controlled-pore glass and the normal and stopped-flow injection modes, achieving linear ranges of the calibration curves between 26-776 mumol/l and 5-265 mumol/l with excellent regression coefficients and good coefficients of variation. The methods have been applied to the determination of this analyte in serum with excellent results.
Fernández-Romero
J M
JM
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
de Castro
L
L
Valcárcel
M
M
D002784:Cholesterol / Q000097:blood*; D004800:Enzymes, Immobilized; D006801:Humans; D007202:Indicators and Reagents; D010783:Photometry; D013050:Spectrometry, Fluorescence
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D002784:Cholesterol