Drug cytotoxicity at elevated temperature. In vitro study on the U-87MG glioma cell line.
The malignant glioma cell line U-87MG was used for 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), aziridinylbenzoquinone (AZQ), cis-diaminodichloroplatinum (II) (cis-DDP), and spirohydantoin mustard (SHM) treatments at 37 degrees and 42 degrees C. With the exception of SHM, all drugs killed a greater proportion of cells at the higher temperature, as assessed by the colony-formation assay. Drug-dose enhancement ratios were 1.6, 2.8, 2, and 1:1 for BCNU, AZQ, cis-DDP, and SHM, respectively. Because methods to heat discrete volumes of brain are now available, we conclude that hyperthermic increase of BCNU, AZQ, and cis-DDP cytotoxicity might have therapeutic application for malignant gliomas.
Da Silva
V F
VF
Department of Surgery (Neurosurgery), University of Ottawa, Ontario, Canada.
Raaphorst
G P
GP
Goyal
R
R
Feeley
M
M
D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001388:Aziridines / Q000008:administration & dosage; D016227:Benzoquinones; D001932:Brain Neoplasms / Q000188:drug therapy*; D002330:Carmustine / Q000008:administration & dosage; D002460:Cell Line; D002945:Cisplatin / Q000008:administration & dosage; D053138:Cyclohexenes; D005910:Glioma / Q000188:drug therapy*; D006358:Hot Temperature / Q000627:therapeutic use*; D006801:Humans; D006827:Hydantoins / Q000008:administration & dosage; D066298:In Vitro Techniques; D009588:Nitrogen Mustard Compounds / Q000008:administration & dosage
D000970:Antineoplastic Agents; D001388:Aziridines; D016227:Benzoquinones; D053138:Cyclohexenes; D006827:Hydantoins; D009588:Nitrogen Mustard Compounds; C006052:2,5-bis(1-aziridinyl)-3,6-bis(2-methoxyethoxy)-4-benzoquinone; C015511:spiromustine; D002945:Cisplatin; D002330:Carmustine