title: In vitro tetracycline susceptibility of Chlamydia trachomatis in clinical specimens.
abstract: A novel approach to determine the tetracycline susceptibility of Chlamydia trachomatis directly from specimens without cell culture propagation and adaptation has been explored. Out of a total of 1290 genital specimens from a sexually transmitted disease clinic, 211 (16.4%) were positive for C. trachomatis. A tetracycline concentration of 0.032 microgram/ml completely inhibited the appearance of inclusions in all of the 211 positive specimens. Of the positive specimens, 120 (56.9%) and 18 (8.5%) respectively showed the presence of 1 to 9 and 100 or more inclusions per microtiter well in antibiotic free medium. Other antibiotics are being tested in the same manner.
authors: Ontario Ministry of Health, Public Health Laboratory, Ottawa, Canada.
authors: A H
authors: AH
authors: Hsu
authors: B
authors: B
authors: Knelsen
authors: S
authors: S
authors: Kasatiya
mesh_terms: D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000302:isolation & purification; D005835:Genitalia / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D013752:Tetracycline / Q000494:pharmacology*; D013753:Tetracycline Resistance
chemical_list: D013752:Tetracycline