title: [The role of tryptophan residues of NADPH-adrenodoxin reductase in the formation of complex with adrenodoxin].
abstract: Chemical modification of tryptophan residues by N-bromosuccinimide was used to determine the role of these residues in the NADPH-adrenodoxin-catalyzed reduction of adrenodoxin, dichlorophenolindophenol and ferricyanide. It was shown that the rate of reduction of all electron acceptors diminishes with modification of tryptophan residues. The most significant decrease of the enzyme activity is observed in case of adrenodoxin-catalyzed reactions. It was suggested that tryptophan residues are responsible for the adrenodoxin reductase interaction with adrenodoxin.
authors: E G
authors: EG
authors: Sarkisova
authors: S S
authors: SS
authors: Mardanian
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology / Q000378:metabolism; D000325:Adrenodoxin / Q000378:metabolism*; D000818:Animals; D001974:Bromosuccinimide / Q000494:pharmacology; D002384:Catalysis; D002417:Cattle; D004579:Electron Transport; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D000325:Adrenodoxin; D009249:NADP; D014364:Tryptophan; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases; D001974:Bromosuccinimide
vernacular_title: Rol' ostatkov triptofana NADPH-adrenodoksinreduktazy v obrazovanii kompleksa s adrenodoksinom.