LD50 determination: objections to the method of Beccari as modified by Molinengo.
The efficiency of the method of Beccari as modified by Molinengo is compared with the sequential technique of Dixon and Mood. It is concluded that the sequential technique is less constrained, gives estimates that are less disperse and is simpler to analyze statistically than the regression of Beccari-Molinengo. Both techniques are comparable in the number of animals required, as well as in the time invested in each assay.
Sevcik
C
C
Laboratory of Cellular Neuropharmacology, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas, Venezuela.
D000826:Animal Testing Alternatives; D000818:Animals; D000997:Antivenins / Q000592:standards / Q000633:toxicity; D007928:Lethal Dose 50; D051379:Mice; D009010:Monte Carlo Method; D014688:Venoms / Q000592:standards / Q000633:toxicity
D000997:Antivenins; D014688:Venoms