On the molecular mechanism of DNA translocation during in vitro packaging of bacteriophage T3 DNA.
The process of packaging of bacteriophage T3 DNA in a defined in vitro system can be separated into two stages: formation of a precursor complex (50 S complex) in the presence of adenosine-5'-O-(3'-thiotriphosphate) (ATP-gamma-S) and subsequent translocation of DNA into the head by the addition of ATP. Packaged DNA exits when DNA translocation is interrupted by the addition of ATP-gamma-S (M. Shibata, H. Fujisawa, and T. Minagawa, 1987, Virology, in press; M. Shibata, H. Fujisawa, and T. Minagawa, 1987, J. Mol. Biol., in press). The in vitro system packaged nicked and cross-linked DNAs but did not package single-stranded DNA. DNA packaging was inhibited by intercalating reagents such as ethidium bromide, acridine orange, and 4',6-diamino-2-phenylindole dihydrochloride. The inhibitory effect was proportional to the ability of intercalating agents to unwind DNA. Ethidium bromide did not inhibit the formation of 50 S complex but blocked translocation of DNA into and out of the capsid. DNA packaging was inhibited by actinomycin D and distamycin A which bind to the minor groove of the DNA helix. From these results, we conclude that DNA packaging mechanism utilizes the exterior structure of duplex DNA for translocating the DNA into the capsid.
Fujisawa
H
H
Department of Botany, Faculty of Science, Kyoto University, Japan.
Hamada
K
K
Shibata
H
H
Minagawa
T
T
D002213:Capsid / Q000235:genetics; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000187:drug effects / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000494:pharmacology; D013604:T-Phages / Q000235:genetics* / Q000378:metabolism
D004279:DNA, Viral; D004996:Ethidium