Chorionic villus sampling: improved method for preparation of karyotypes after short-term incubation.
A method for the isolation and trypsin-Giemsa banding of metaphases obtained after short-term incubation (48 h) of cytotrophoblast cells from chorionic villus sample is described. A new slide-making instrument, developed expressly to enhance the spreading of chromosomes from metaphases released from small tissue pieces, is responsible for the increase yield of analysable metaphases in this protocol.
Terzoli
G L
GL
Laboratorio di Citogenetica Instituti Clinici di Perfezionamento, Milano, Italy.
Lalatta
F
F
Gilbert
F
F
D002824:Chorionic Villi; D002871:Chromosome Banding / Q000379:methods; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D011247:Pregnancy