Purification of saikosaponins a, c and d. Application of large-scale reversed high-performance liquid chromatography.
The preparative separation of saikosaponins a, c, and d from Bupleurum falcatum was performed by high-performance liquid chromatography on a 100 X 11 cm I.D. axially compressed column, packed with 20-micron ODS silica gel using a step gradient with mixtures of acetonitrile and water as the mobile phase. Gram quantities of pure compounds were obtained in a single run.
Sakuma
S
S
Chromatographic Division, Kurita Water Industries Ltd., Tokyo, Japan.
Motomura
H
H
D002851:Chromatography, High Pressure Liquid; D007564:Japan; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010946:Plants, Medicinal / Q000032:analysis; D012502:Sapogenins / Q000302:isolation & purification*; D012503:Saponins
D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D