Protein determination in materials containing melanin.
Strategy of protein determination in melanin containing samples was analyzed, and the prerequisites under which the Lowry procedure could be used were established. (a) Complete solubilization of proteins from melanosomes was not reached until the treatment with 3% sodium dodecylsulfate at 100 degrees C was prolonged to 5 h. (b) It is necessary to correct the data obtained with Folin reaction to eliminate melanin interference. (c) The correction is based upon the determination of the extent of solubilized melanin and subtraction of the corresponding Folin-Ciocalteu reaction values. (d) The extent of correction due to the melanin interference was found to be about 40% of the absorbance value. It was concluded that correction of the data obtained with Lowry assay is possible, but the procedure is complicated and time-consuming. However, a more simple modification has not been developed.
Vedralová
E
E
Department of Biochemistry, Faculty of Medicine, Charles University, Prague, Czechoslovakia.
Borovanský
J
J
Duchon
J
J
D000818:Animals; D006224:Cricetinae; D007202:Indicators and Reagents; D007700:Kinetics; D008543:Melanins; D008544:Melanocytes / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
D007202:Indicators and Reagents; D008543:Melanins; D011506:Proteins