Degradation 1,2-dimethylbenzene by Corynebacterium strain C125.
In an attempt to obtain bacteria growing on 1,2-dimethylbenzene as sole carbon and energy source two different strains were isolated. One was identified as an Arthrobacter strain, the other as a Corynebacterium strain. Corynebacterium strain C125 was further investigated. The organism was not capable to grow on 1,3- and 1,4-dimethylbenzene. cis-1,2-Dihydroxycyclohexa-3,5-diene oxidoreductase and 3,4-dimethylcatechol-2,3-dioxygenase activity was found in cell extracts. When 3,4-dimethylcatechol was added to cell extract of 1,2-dimethylbenzene-grown cells, first a compound with the spectral properties of 2-hydroxy-5-methyl-6-oxo-2,4-heptadienoate was formed and subsequently acetate was produced. It is proposed that dioxygenases are involved in the initial steps of 1,2-dimethylbenzene degradation, and ring opening proceeds via meta-cleavage.
Schraa
G
G
Department of Microbiology, Agricultural University, Wageningen, The Netherlands.
Bethe
B M
BM
van Neerven
A R
AR
Van den Tweel
W J
WJ
Van der Wende
E
E
Zehnder
A J
AJ
D001173:Arthrobacter / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D055598:Chemical Phenomena; D002621:Chemistry; D003352:Corynebacterium / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D010101:Oxygen Consumption; D014871:Water Microbiology; D014992:Xylenes / Q000378:metabolism*
D014992:Xylenes; C026114:2-xylene