title: An enzyme-linked lectin-binding assay on cells (CELLBA) for the comparison of lectin receptor expression on cell surfaces.
abstract: Lectins can be used to specifically detect some cell surface glycans. Their expression on different cells or on cells of a given lineage throughout differentiation or following treatment with drugs can be compared using lectins labelled with radioactive, fluorescent or enzymatic probes. We describe a new method which, by analogy with CELISA (ELISA on cells), is called CELLBA (or ELLBA on cells) for cellular, enzyme-linked lectin-binding assay. It permits the comparison of the expression of specific glycans in a large number of different cell samples. As an example, it was able to detect alterations of cell surface glycan expression caused by inhibitors of N-linked oligosaccharide trimming.
authors: Preclinical Research Department, Sandoz Ltd., Basel, Switzerland.
authors: C
authors: C
authors: Gavériaux
authors: F
authors: F
authors: Loor
mesh_terms: D000371:Agglutination; D000818:Animals; D001710:Biotin; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006001:Glycoconjugates / Q000032:analysis*; D037102:Lectins / Q000378:metabolism*; D008361:Mannosidases / Q000037:antagonists & inhibitors; D051379:Mice; D009005:Monosaccharides / Q000378:metabolism; D010969:Plastics; D051381:Rats; D011975:Receptors, Mitogen / Q000032:analysis*
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D009005:Monosaccharides; D010969:Plastics; D011975:Receptors, Mitogen; D001710:Biotin; D008361:Mannosidases