Highly sensitive determination of chlorpheniramine as fluorescence derivative by high-performance liquid chromatography.
A new method has been developed for the quantitative analysis of chlorpheniramine in human blood using high-performance liquid chromatography with a fluorescence detector. Benzyl chloroformate was found to be suitable as a fluorometric derivatizing reagent. A linear calibration curve ranging from 0 to 40 ng was obtained for chlorpheniramine, and the minimum detectable concentration was 0.1 ng/ml (whole blood) at a signal-to-noise ratio of 2. It was confirmed that chlorpheniramine levels in the blood of healthy adult volunteers could be precisely determined up to 96 h after a single oral administration of a tablet containing 4 mg of chlorpheniramine.
Miyamoto
Y
Y
Pharmacochemistry Section, Japan Air Self Defense Force, Tokyo, Japan.
D002744:Chlorpheniramine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005561:Formates; D006801:Humans; D008832:Microchemistry; D013050:Spectrometry, Fluorescence
D005561:Formates; C018241:carbobenzoxy chloride; D002744:Chlorpheniramine