title: Morphological response of injured adult rabbit optic nerve to implants containing media conditioned by growing optic nerves.
abstract: Adult rabbit retina can express regeneration-associated characteristics after optic nerve injury, provided it is supplied with appropriate diffusible substances originating from media conditioned by regenerating fish optic nerves or by optic nerves of a newborn rabbit [Hadani et al., Proc. Natl. Acad. Sci. U.S.A., 81 (1984) 7965; Schwartz et al., Science, 228 (1985) 600]. This was shown by applying the active substances to the injured axons in the form of 'wrap-around' implants, consisting of collagen-coated silicone tubes which had been soaked in the conditioned media (CM). The regeneration-associated response was manifested biochemically and by sprouting of nerve fibers in culture. The present work provides morphological evidence that the implantation prolongs survival of ganglion cells and optic nerve fibers and induces new growth. Light microscopic analysis (using horseradish peroxidase (HRP) for labeling the fibers) revealed, 1 week following optic nerve injury, labeled fibers and ganglion cells in both the implanted and control (injured only or injured and implanted with collagen-coated silicone tubes free of CM) nerves. However, from the second week after the injury, distinct differences in the appearance of viable ganglion cells and labeled fibers, were seen between experimental and control preparations. In sections taken through the optic nerve, at the region distal to the site of injury, HRP-labeled fibers were seen in the experimental nerves 1 week, 2 weeks and to a significantly lesser extent 1 month after injury.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
authors: V
authors: V
authors: Lavie
authors: A
authors: A
authors: Harel
authors: A
authors: A
authors: Doron
authors: A
authors: A
authors: Solomon
authors: D
authors: D
authors: Lobel
authors: M
authors: M
authors: Belkin
authors: S
authors: S
authors: Ben-Basat
authors: S
authors: S
authors: Sharma
authors: M
authors: M
authors: Schwartz
mesh_terms: D000818:Animals; D002470:Cell Survival; D005399:Fishes; D008854:Microscopy, Electron; D009416:Nerve Regeneration / Q000187:drug effects*; D009900:Optic Nerve / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D011817:Rabbits; D012160:Retina / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000502:physiology* / Q000648:ultrastructure; D014020:Tissue Extracts
chemical_list: D014020:Tissue Extracts