The genotoxic effect of beta-propiolactone on mammalian oocytes.
beta-Propiolactone (beta PL) has been tested on preimplantation mouse embryos for possible genotoxic effects. Tests were performed at different stages of meiosis (late prophase I, diakinesis/metaphase I, anaphase I, telophase I/prophase II and metaphase II) by injecting females at various times after the induction of superovulation. Male and female derived chromosome complements from first-cleavage embryos were analysed before syngamy for cytogenetic abnormalities. A higher proportion of diploid oocytes, produced by the non-extrusion of the first or second polar body, was found after fertilization when the compound was administered immediately before metaphase I or II. No obvious effect was detected at any other time of beta PL exposure. Based on these results, several possible modes of action for beta PL are postulated.
Santaló
J
J
Departament de Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, Spain.
Estop
A M
AM
Egozcue
J
J
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects*; D005260:Female; D007621:Karyotyping; D007783:Lactones / Q000633:toxicity*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens; D009865:Oocytes / Q000187:drug effects* / Q000648:ultrastructure; D011247:Pregnancy; D011420:Propiolactone / Q000633:toxicity*
D007783:Lactones; D009153:Mutagens; D011420:Propiolactone