The effect of the non-ionic surfactant Brij 30 on the cytotoxicity of adriamycin in monolayer, spheroid and clonogenic culture systems.
The effects of a non-ionic polyoxyethylated lauryl ether surfactant (Brij 30) on monolayer uptake and spheroid penetration of adriamycin have been studied. Co-incubation of adriamycin with Brij 30 increases intracellular adriamycin levels by 2-3-fold. Although, in the concentrations used, Brij 30 alone is not cytotoxic, adriamycin and Brij 30 mixtures are significantly more cytotoxic (monolayer ID90 = 0.6 microgram/ml; disaggregated spheroid ID50 = 1.9 micrograms/ml) and induce significantly longer spheroid growth delay than adriamycin alone (monolayer ID90 = 2.1 micrograms/ml; disaggregated spheroid ID50 = 3.3 micrograms/ml). Adriamycin is equally cytotoxic to mouse normal granulocytes and chronic myeloid leukaemic (M1 cell line) cells in agar clonogenic cultures. The addition of Brij 30 appears to enhance preferentially the activity of adriamycin against these tumour cells relative to the normal granulocytes.
Kerr
D J
DJ
Department of Medical Oncology, Glasgow University, UK.
Wheldon
T E
TE
Russell
J G
JG
Maurer
H R
HR
Florence
A T
AT
Halbert
G W
GW
Freshney
R I
RI
Kaye
S B
SB
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D004357:Drug Synergism; D006098:Granulocytes / Q000187:drug effects; D006801:Humans; D051379:Mice; D000077423:Polidocanol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D013997:Time Factors
D003902:Detergents; D013501:Surface-Active Agents; D000077423:Polidocanol; D011092:Polyethylene Glycols; D004317:Doxorubicin