Enzymatic deacylations of esterified saccharides. II. De-esterifications of radiolabelled O-acylglucopyranosides by mice serum and liver esterases.
1. 14C-Labelled methyl 2,6-di-O-pivaloyl-alpha-D-glucopyranoside (1) was used as a novel substrate for esterases from mouse serum and liver. 2. Stepwise de-esterification of the diester substrate 1 was achieved, and data on time-course experiments are reported. 3. Kinetic studies were undertaken to compare deacylation rates for the enzymatic de-esterification of the diester substrate 1 using both, mice sera and liver microsomal fractions. 4. Serum and liver esterase activities were studied in mice treated with an immunostimulating agent, peptidoglycan monomer (PGM), and a comparison made with esterases from untreated mice.
Tomić
S
S
Department of Radioimmunology, Institute of Immunology, Zagreb, Yugoslavia.
Trescec
A
A
Tomasić
J
J
D000215:Acylation; D000818:Animals; D002250:Carbon Radioisotopes; D004950:Esterases / Q000097:blood / Q000378:metabolism*; D005260:Female; D005960:Glucosides; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL
D002250:Carbon Radioisotopes; D005960:Glucosides; D004950:Esterases