title: Characterization of amino acid transport during erythroid cell differentiation.
abstract: We have studied the changes in amino acid transport in fetal erythroid cells isolated from rat fetal liver at different gestation days. Our results show that System A transport as measured by the Na+-dependent uptake of 2-(methylamino)isobutyric acid (MeAIB) was conspicuous at day 13 but virtually disappeared between days 16 and 18. In contrast, the activity of System ASC measured by the Na+-dependent uptake of MeAIB-insensitive threonine uptake increased after day 14 and was optimal between days 16 and 18. This transport system regressed in activity with further maturation, but remained conspicuously saturable in the matured red blood cell. Interestingly, the newly discovered Na+-independent System asc (Vadgama, J. V., and Christensen, H.N. (1985) J. Biol. Chem. 260, 2912-2921), selective for the uptake of test substrates threonine, serine, and alanine, was present in these erythroid cells. Its activity increased during gestation days 16-18. System L transport was present simultaneously with the Na+-independent System asc. As we had previously demonstrated for the pigeon red blood cell, these two transport systems are kinetically independent as confirmed with inhibition studies and the special selectivity of System L to trans stimulation. Tryptophan uptake could be attributed predominantly to System L, as also observed for the nucleated pigeon red blood cells and certain other cells. Arginine showed its familiar Na+-independent mode of uptake as a cation throughout the interval of study. An exceptional Na+-dependent component of arginine uptake emerged after day 14, peaked at day 18, and then disappeared on further maturation of the erythroid cell.
authors: Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109.
authors: J V
authors: JV
authors: Vadgama
authors: M
authors: M
authors: Castro
authors: H N
authors: HN
authors: Christensen
mesh_terms: D000596:Amino Acids / Q000097:blood* / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002454:Cell Differentiation; D005314:Embryonic and Fetal Development; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005312:Fetal Blood; D007700:Kinetics; D008099:Liver / Q000196:embryology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D012964:Sodium