[Nonenzymatic incorporation of prostaglandin A1 into phospholipids in rat liver microsomes].
The incorporation of [5,6(n)-3H]prostaglandin A1 (PGA1) and [1-14C]oleic acid into membrane phospholipids of rat liver microsomes was studied. It was shown that PGA1 is incorporated into phospholipids in a much lesser degree than oleic acid. PGA1 is incorporated into phosphatidylethanolamine and, in a lesser degree, into phosphatidylcholine and phosphatidylinositol + phosphatidylserine. The exogenous cofactors of fatty acid acylation (ATP, CoA, Mg2+) exert no marked influence on the incorporation of PGA1 into the phospholipids. PGA1 interacts with isolated rat liver phospholipids; the PGA1-phospholipid conjugate formed is not destroyed in the course of one- or two-dimensional thin-layer chromatography. On the other hand, PGA1 binding to unsaturated phosphatidylcholines is strictly dependent on the phospholipid oxidation index. It is concluded that PGA1 incorporation into rat liver phospholipids is a result of interaction of PGA1 with peroxidized phospholipids.
Kulikov
V I
VI
Levachev
S M
SM
D000818:Animals; D002855:Chromatography, Thin Layer; D006900:Hydroxylation; D008054:Lipid Peroxides / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D009829:Oleic Acids / Q000378:metabolism; D010084:Oxidation-Reduction; D011454:Prostaglandins A / Q000378:metabolism*; D051381:Rats
D008054:Lipid Peroxides; D008563:Membrane Lipids; D009829:Oleic Acids; D011454:Prostaglandins A; C100573:prostaglandin A1
Nefermentativnoe vkliuchenie prostaglandina A1 v fosfolipidy mikrosom pecheni krysy.