Sequence analysis and expression of an X-linked, lymphocyte-regulated gene family (XLR).
The XLR gene family consists of approximately 10 X-linked genes, the expression of which is regulated in lymphocyte development. Certain members of the gene family are closely linked to the murine xid immune deficiency mutation. Sequence analysis of a cDNA clone pM1 derived from the plasmacytoma MOPC167 showed an open reading frame capable of coding for a protein of 208 amino acids and mol wt 24,000. The lack of a signal peptide or transmembrane region indicates a probable cytoplasmic or nuclear localization for the predicted pM1 protein. The predicted protein shares significant homology with lamins A and C and other members of the intermediate filament family of proteins, and shares features important for the coiled-coil structure proposed for these proteins. Analysis of cDNA clones derived from a presecretory lymphoma and from adult thymus indicates that B and T lymphocytes transcribe a common major mRNA identical to pM1, while other rare transcripts were also identified by these studies. A series of clonal T lymphoma lines representing distinct stages of thymic differentiation showed that, as with B lymphoid tumors, XLR expression is correlated with the maturation of the thymomas.
Siegel
J N
JN
Laboratory of Chemical Biology, National Institute of Diabetes, and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Turner
C A
CA
Klinman
D M
DM
Wilkinson
M
M
Steinberg
A D
AD
MacLeod
C L
CL
Paul
W E
WE
Davis
M M
MM
Cohen
D I
DI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D007153:Immunologic Deficiency Syndromes / Q000235:genetics*; D007381:Intermediate Filament Proteins / Q000235:genetics; D034882:Lamins; D008214:Lymphocytes / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics; D014960:X Chromosome
D007381:Intermediate Filament Proteins; D034882:Lamins; D009687:Nuclear Proteins; D004247:DNA