title: Characterization of alternate reductant binding and electron transfer in the dopamine beta-monooxygenase reaction.
abstract: The steady-state limiting kinetic parameters Vmax, V/KDA, and V/KO2, together with deuterium isotope effects on these parameters, have been determined for the dopamine beta-monooxygenase (D beta M) reaction in the presence of structurally distinct reductants. The results show the one-electron reductant ferrocyanide to be nearly as kinetically competent as the presumed in vivo reductant ascorbate. Further, a reductant system of ferricyanide plus substrate dopamine yields steady-state kinetic parameters and isotope effects very similar to those measured solely in the presence of ferrocyanide, indicating a role for catecholamine in the rapid recycling of oxidized ferrocyanide. Use of substrate dopamine as the sole reductant is found to lead to a highly unusual kinetic independence of oxygen concentration, as well as significantly reduced values of Vmax and V/KDA, and we conclude that dopamine reduces enzymic copper in a rate-limiting step that is 40-fold slower than with ascorbate. The near-identical kinetic parameters measured in the presence of either ascorbate or ferrocyanide, together with markedly reduced rates with dopamine, are interpreted in terms of a binding site for reductant that is physically distinct from the substrate binding site. This view is supported by molecular modeling, which reveals ascorbate and ferrocyanide to possess an unexpected similarity in potential sites for interaction with enzymic residues. With regard to electron flux, identical values of V/KO2 have been measured with [2,2-2H2]dopamine as substrate both in the presence and in the absence of added ascorbate.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Chemistry, University of California, Berkeley 94720.
authors: L C
authors: LC
authors: Stewart
authors: J P
authors: JP
authors: Klinman
mesh_terms: D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001665:Binding Sites; D002417:Cattle; D004298:Dopamine / Q000378:metabolism; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D004579:Electron Transport; D005292:Ferricyanides / Q000378:metabolism; D005295:Ferrocyanides / Q000378:metabolism; D007700:Kinetics; D008433:Mathematics; D008968:Molecular Conformation; D011485:Protein Binding
chemical_list: D005292:Ferricyanides; D005295:Ferrocyanides; D004299:Dopamine beta-Hydroxylase; D001205:Ascorbic Acid; D004298:Dopamine