[Electron-autoradiographic demonstration of intra- and extracellular bactericidal activity of polymorphonuclear leukocytes].
A technique for the determination of intra- and extra-cellular bactericidal activity of neutrophils has been suggested. A mixture of neutrophils and bacteria was incubated for 30 min and subsequently 3H-uridine was added to the incubated mixture. If phagocytized and extracellularly located bacteria remain alive they incorporate 3H-uridine. Killed bacteria fail to incorporate 3H-uridine. Intra- and extracellular killing capacity of neutrophils is determined by the content of labelled and unlabelled bacteria in radioautographs.
Pal'tsyn
A A
AA
Chervonskaia
N V
NV
Badikova
A K
AK
Uchaneishvili
E K
EK
D001345:Autoradiography; D001419:Bacteria / Q000378:metabolism; D001770:Blood Bactericidal Activity; D006801:Humans; D008854:Microscopy, Electron; D009504:Neutrophils / Q000502:physiology*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D014316:Tritium; D014529:Uridine
Elektronno-radioavtograficheskoe vyiavlenie intra- i ékstratselliuliarnogo bakteritsidnogo deĭstviia polimorfnoiadernykh leĭkotsitov.