Radiometric-high-performance liquid chromatographic assay for nicotine and twelve of its metabolites.
A sensitive, reproducible radiometric-high-performance liquid chromatographic assay has been developed to measure concentrations of nicotine and twelve of its metabolites in biological fluids. Following administration of nicotine ([2-14C]pyrrolidine) to rats, the assay was used in a pharmacokinetic investigation.
Kyerematen
G A
GA
Department of Pharmacology, Pennsylvania State University, College of Medicine, Hershey 17033.
Taylor
L H
LH
deBethizy
J D
JD
Vesell
E S
ES
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008297:Male; D009538:Nicotine / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D007202:Indicators and Reagents; D009538:Nicotine