title: In vitro gliadin antibody production by peripheral blood mononuclear cells from patients with coeliac disease.
abstract: In vitro spontaneous IgG gliadin antibody production was shown in peripheral blood mononuclear cell (PBMC) cultures from 12/14 patients with active coeliac disease; in most cases no increase and sometimes a marked reduction of the in vitro synthesis was observed after pokeweed mitogen (PWM) addition. Lower levels of gliadin antibodies were also detected in PBMC cultures from 7/12 coeliac patients in remission; in all the cases the synthesis was increased by PWM. In vitro production was confirmed by higher levels in 7-day culture supernatants than in 0-day frozen-thawed cell pellets and by the inhibitory effect of cycloheximide. Spontaneous release of antibodies occurred within the first 3 days of culture, while PWM-induced antibody production reached a plateau after 7-9 days. The analysis of the in vitro gliadin antibody production is a promising technique to assess the regulatory mechanisms involved in the humoral immune response to gliadin.
authors: Department of Pediatrics, 2nd Faculty of Medicine, University of Naples, Italy.
authors: R
authors: R
authors: Troncone
authors: E
authors: E
authors: Farris
authors: A
authors: A
authors: Donatiello
authors: S
authors: S
authors: Auricchio
mesh_terms: D000293:Adolescent; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D002446:Celiac Disease / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003513:Cycloheximide / Q000494:pharmacology; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007223:Infant; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D010940:Plant Proteins / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013997:Time Factors
chemical_list: D010940:Plant Proteins; D011043:Pokeweed Mitogens; D005903:Gliadin; D003513:Cycloheximide