Adhesion of cultured human endothelial cells onto methacrylate polymers with varying surface wettability and charge.
The adhesion of human endothelial cells (HEC) onto a series of well-characterized methacrylate polymer surfaces with varying wettabilities and surface charges was studied either in serum-containing (CMS) or in serum-free (CM) culture medium. HEC adhesion in CMS onto (co)polymers of hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA) was found to be optimal on the moderately wettable copolymer (mol ratio 25 HEMA/75 MMA). Positively-charged copolymers of HEMA or MMA with trimethylaminoethyl methacrylate-HCl salt (TMAEMA-Cl), both with mol ratios of 85/15 and a negatively-charged copolymer of MMA with methacrylic acid (MAA), mol ratio 85/15, showed high numbers of adhering HEC. In CM, HEC adhered onto the three charged copolymers mentioned above, but neither onto the copolymer of HEMA and MAA (mol ratio 85/15) nor onto the HEMA/MMA co- and homopolymers. Complete cell spreading in CM was only observed on the positively-charged copolymers.
van Wachem
P B
PB
Department of Chemical Technology, Twente University of Technology, Enschede, The Netherlands.
Hogt
A H
AH
Beugeling
T
T
Feijen
J
J
Bantjes
A
A
Detmers
J P
JP
van Aken
W G
WG
D000179:Acrylates; D002448:Cell Adhesion; D002478:Cells, Cultured; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008689:Methacrylates; D011108:Polymers; D013499:Surface Properties
D000179:Acrylates; D003470:Culture Media; D008689:Methacrylates; D011108:Polymers