Measurement of glycated albumin in diabetic patients by biospecific affinity chromatography.
The percentage of glycated plasma albumin was measured by a procedure involving ammonium sulphate precipitation and Affi-Gel-Blue and phenylboronate chromatographies. The value correlates well with the amount of ketoamine-bound sugars determined by colorimetric assay (r = 0.98, n = 39). The normal mean value is 3.9 +/- 0.3% (mean +/- S.D., coefficient of variation = 7.7%, n = 32) and varies from 3.9 to 21% in diabetics (n = 54). A good correlation is found with the mean blood glucose value of the preceding twenty days (r = 0.92, n = 57). Because of its relative ease of determination, glycated albumin constitutes a good short-term glycemic index and an alternative to glycated haemoglobin in some specific cases.
Ducrocq
R
R
Laboratoire de Biochimie B, Hôpital Bichat, Paris, France.
Le Bonniec
B
B
Carlier
O
O
Assan
R
R
Elion
J
J
D001786:Blood Glucose / Q000378:metabolism; D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D017127:Glycation End Products, Advanced; D006801:Humans; D012709:Serum Albumin / Q000032:analysis* / Q000302:isolation & purification; D000093423:Glycated Serum Albumin
D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D000093423:Glycated Serum Albumin