Identification of drugs in human serum by high-performance liquid chromatography with photodiode array detection and a search algorithm for ultraviolet spectra.
A system is described that identifies a number of unknown drugs (benzodiazepines, antidepressants and neuroleptics) in blood specimens for clinical-toxicological purposes. Reversed ion-pair high-performance liquid chromatography with a photodiode array detector saves the ultraviolet spectrum of every chromatographically significant peak. Post-run data-processing, provided by a microcomputer, retrieves candidate substances from a library of ultraviolet spectra. Selected standard ultraviolet spectra are compared with the unknown by five different similarity tests. The discriminatory efficiency of these algorithms has been determined. Multicomponent analysis, a built-in program of the spectrophotometer, provided the most reliable results.
Minder
E I
EI
Central Laboratory of Clinical Chemistry, University Hospital, Zurich, Switzerland.
Schaubhut
R
R
Minder
C E
CE
Vonderschmitt
D J
DJ
D000465:Algorithms; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006801:Humans; D007256:Information Systems; D004364:Pharmaceutical Preparations / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation
D004364:Pharmaceutical Preparations