[Evaluation of an experimental animal model allowing the study of the cecal microflora in the hamster, antagonistic to clostridium difficile].
The purpose of this study was the development and evaluation of an experimental model allowing the investigation of hamster anti-Clostridium difficile coecal microflora. The existence of this "barrier" was verified in conventional hamsters. Such hamster coecal flora was then orally transferred to C3H germ-free mice. In such animals, the "barrier effect" was maintained. After treatment with erythromycin, the colonization resistance was always maintained; despite two subsequent processes, dilutions of coecal contents (10(-2] and subsequent heating of this fluid (70 degrees C, 10 min), the inhibitory activity against C. difficile was partially maintained (10(4) UFC/g faeces). The isolation of anaerobic strains implicated in colonization resistance will next be carried out in an anaerobic chamber using this microflora.
Su
W J
WJ
Laboratoire de Microbiologie, Centre d'Etudes Pharmaceutiques, Chatenay Malabry, France.
Bourlioux
P
P
Bournaud
M
M
Besnier
M O
MO
Fourniat
J
J
D000818:Animals; D002432:Cecum / Q000382:microbiology*; D003013:Clostridium / Q000187:drug effects*; D006224:Cricetinae; D004917:Erythromycin / Q000494:pharmacology*; D006361:Heating; D051379:Mice; D008809:Mice, Inbred C3H; D008954:Models, Biological
D004917:Erythromycin
Mise au point d'un modèle expérimental animal permettant l'etude de la microflore coecale du hamster, antagoniste de Clostridium difficile.