Determination of theophylline and its metabolites in plasma and urine by reversed-phase liquid chromatography using an amine modifier.
A high-performance liquid chromatographic method for the determination of the concentrations of theophylline and its metabolites in plasma and urine samples is presented. The method uses the decylammonium ion, an N-alkylammonium modifier, and makes it possible to separate theophylline and its metabolites from other uric acid or xanthine derivatives, especially 1,7-dimethylxanthine, a metabolite of caffeine. The plasma and urine purification procedure is convenient, rapid and reproducible, and it can be used to determine low plasma and urine concentrations.
Naline
E
E
Département de Biochimie et de Pharmacologie, Hôpital A. Mignot, Le Chesnay, France.
Flouvat
B
B
Advenier
C
C
Pays
M
M
D000097:Acetonitriles; D000588:Amines; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013806:Theophylline / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D014970:Xanthines / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine
D000097:Acetonitriles; D000588:Amines; D014970:Xanthines; D013806:Theophylline; C004744:decylamine