Purification and characterization of aplysianin E, an antitumor factor from sea hare eggs.
An antitumor factor, aplysianin E, inducing tumor lysis was purified to apparent homogeneity from the supernatant of a homogenate of eggs of the sea hare Aplysia kurodai. Purified aplysianin E was a 250-kDa glycoprotein containing three different subunits. This factor was half-maximally active at 2-114 ng protein/ml and lysed all the tumor cells tested but did not lyse normal white or red blood cells. Aplysianin E was labile on treatments with heat, low pH, urea, guanidine, sodium lauryl sulfate, and periodate, but not with proteases or organic solvents, Aplysianin E completely inhibited the syntheses of DNA, RNA, and protein by tumor cells within 2 h and caused their complete cytolysis within 15 h. Tumor lysis by aplysianin E was inhibited by N-acetylneuraminic acid, suggesting that recognition of the sugar moiety is a key step in cytolysis induced by aplysianin E. Aplysianin E also prolonged the survival of mice bearing syngeneic MM46 ascites or solid tumors. These results suggest that aplysianin E, found in an invertebrate, the sea hare, is a new antitumor factor.
Kisugi
J
J
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Kamiya
H
H
Yamazaki
M
M
D000818:Animals; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D001048:Aplysia / Q000032:analysis*; D006020:Glycopeptides; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000188:drug therapy; D010063:Ovum / Q000032:analysis; D013696:Temperature
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D006020:Glycopeptides; D006023:Glycoproteins; C053778:aplysianin E