Comparison of chromogranins A, B, and secretogranin II in human adrenal medulla and pheochromocytoma.
Human phenochromocytomas were analyzed for the presence of chromogranins A and B and secretogranin II (chromogranin C). In immunohistochemistry, a positive staining of the tumor cells was observed for all three antigens. For immunoblotting, a method had to be developed which prevented proteolytic degradation of the antigens. Boiling homogenates of freeze-dried tissues achieved this and in addition, led to a significant enrichment of these secretory proteins. All three antigens could be identified in the tumor by immunoblotting. Chromogranin A and B were the major components present in about equal amounts. The relative concentration of all these antigens in the tumors was similar to those in adrenal medulla. A method involving high pressure liquid chromatography is presented which allows the separation of the human chromogranins/secretogranins.
Schober
M
M
Department of Pharmacology, University of Innsbruck, Austria.
Fischer-Colbrie
R
R
Schmid
K W
KW
Bussolati
G
G
O'Connor
D T
DT
Winkler
H
H
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D002864:Chromogranins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007118:Immunoassay; D007150:Immunohistochemistry; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010673:Pheochromocytoma / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*
D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D011506:Proteins