Association of polymorphonuclear leukocytes with sites of aortic catheter-induced injury in rabbits.
The kinetics of the association of polymorphonuclear leukocytes (PMNs) with arterial balloon catheter-induced injury have been examined. An average of 6 X 10(7) PMNs were isolated from 20 ml of blood and labelled with 111In-oxine for reinfusion into the donor rabbit. The cells remained viable as demonstrated by both in vitro and in vivo tests of cell function. The abdominal aorta of rabbits was denuded of endothelium and immediately, 24 h, or 5 weeks later, exposed to autologous radiolabelled PMNs for 1 h. The presence of PMNs at sites of denudation was demonstrated by detection of the radioactive label and was confirmed by light and electron microscopy after 24 h, but not at 5 weeks. Immediately following denudation radioactivity was 2.44 +/- 0.33 times control (P = 0.006); 2.52 +/- 0.18 at 24 h (P = 0.005); and 1.88 +/- 0.32 times control at 5 weeks (P = 0.045). The presence of PMNs, or their products, 5 weeks after denudation suggests a more complex role of PMNs and possibly a direct involvement in the long term changes resulting from arterial balloon catheter injury.
Cole
C W
CW
Atherosclerosis Research Laboratory, Duke University Medical Center, Durham, NC 27710.
Hagen
P O
PO
Lucas
J F
JF
Mikat
E M
EM
O'Malley
M K
MK
Radic
Z S
ZS
Makhoul
R G
RG
McCann
R L
RL
D000818:Animals; D001012:Aorta, Abdominal / Q000293:injuries; D001808:Blood Vessels / Q000293:injuries* / Q000473:pathology; D002404:Catheterization; D004730:Endothelium, Vascular / Q000502:physiology; D007205:Indium Radioisotopes; D008297:Male; D008854:Microscopy, Electron; D009504:Neutrophils / Q000502:physiology*; D011817:Rabbits
D007205:Indium Radioisotopes