title: Low-molecular-weight trypsin inhibitors of microbial origin.
abstract: Three hundred actinomyces cultures newly isolated from the soil of different regions of the Soviet Union were tested for their ability to produce inhibitors of trypsin-like proteases. Seven previously not known to produce trypsin inhibitors (Streptomyces bikiniensis 17-5, S. sporoclivatus 28-1, S. filamentosus 32-11, S. diastatochromogenes 20-4, S. lavendulae 29-4, S. violacens 52-8, and Streptoverticillium cinnamoneum 36-8) were found to possess high antitrypsin activity. The morphological and cultural properties of the strains and the dynamics of inhibitor production were investigated. S. bikiniensis 17-5 was studied in greatest detail. Its culture filtrate contained several inhibitors for trypsin and one for chymotrypsin. A mixture of oligopeptides with Mr of 300-500 was obtained by the described procedure which included the adsorption of the culture fluid filtrate on charcoal followed by ion-exchange chromatography on CM-cellulose. Four trypsin inhibitors (Sb-IT1, Sb-IT2, Sb-IT3, and Sb-IT4) were isolated from the mixture in a highly purified state by reversed-phase high-performance liquid chromatography. Sb-IT2 has been recognized as formylhistidylvaline with an Mr of 282. No trypsin inhibitor of this structure has been described previously.
authors: A.N. Bakh Institute of Biochemistry, Academy of Sciences of the USSR, Moscow.
authors: I V
authors: IV
authors: Ulezlo
authors: N A
authors: NA
authors: Kuropatkina
authors: M V
authors: MV
authors: Shulgina
authors: A M
authors: AM
authors: Bezborodov
mesh_terms: D000190:Actinomyces / Q000254:growth & development*; D011480:Protease Inhibitors / Q000302:isolation & purification; D013302:Streptomyces / Q000254:growth & development*; D014361:Trypsin Inhibitors / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D011480:Protease Inhibitors; D014361:Trypsin Inhibitors