III6NeuAcLc4Cer in human SW1116 colorectal carcinoma cells: a possible oncofetal antigen that is not dependent on Lewis gene expression.
Monospecific rabbit antibodies directed against the human milk sialyloligosaccharides III6NeuAcLcOse4 (sialyltetrasaccharide b), IV3NeuAcLcOse4 (sialyltetrasaccharide a), and IV6NeuAcnLc4Ose (sialyltetrasaccharide c) were used to detect their homologous haptens as gangliosides or ganglioside-derived sialyloligosaccharides from the human colorectal carcinoma cell line SW1116. III6NeuAcLc4Cer was first detected in human meconium [P. A. Prieto and D. F. Smith (1985) Arch. Biochem. Biophys. 241, 281-289], and its presence in a total ganglioside fraction of SW1116 cells together with its absence from a total lipid extract of normal human intestinal mucosa are consistent with III6NeuAcLc4Cer being a tumor-associated oncofetal antigen. IV3NeuAcLc4Cer, a ganglioside in human meconium [P. A. Prieto and D. F. Smith (1986) Arch. Biochem. Biophys. 249, 243-253], was also detected in SW1116 cells; an observation that is consistent with its being the immediate precursor to the sialyl-Lea ganglioside in SW1116 cells. Specific antisera against sialylated type 1 oligosaccharide chains whose expression is independent of the Lewis gene fucosyltransferase may be useful diagnostic reagents for oncofetal, carbohydrate antigens.
Law
K L
KL
Department of Biochemistry and Nutrition, Virginia Tech, Blacksburg 24061.
Smith
D F
DF
D000906:Antibodies; D000951:Antigens, Neoplasm; D002240:Carbohydrate Sequence; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology*; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D009844:Oligosaccharides / Q000302:isolation & purification; D012004:Rectal Neoplasms / Q000276:immunology*
D000906:Antibodies; D000951:Antigens, Neoplasm; D006017:Glycolipids; D009844:Oligosaccharides; C031192:oncofetal antigens; C009255:sialoglycolipids; C016735:sialooligosaccharides