title: [Pre-steady state kinetics of the ferroxidase reaction catalyzed by ceruloplasmin].
abstract: Analysis of the presteady-state step of the ferroxidase reaction catalyzed by ceruloplasmin revealed that the kinetic curve for the reaction product accumulation versus time is characterized by an induction period, tau, whose value does not change within the pH range of 5.0-7.5. The value of tau decreases with a rise in the enzyme concentration and increases with a rise in the substrate concentration at constant Fe(II) and E0 concentrations. A reaction scheme is proposed which points to the existence of an induction period in the ceruloplasmin-catalyzed ferroxidase reaction. It was shown that the calculated values of tau = f([Fe(II)]0) and tau = f([E]0) obtained through the use of differential equations qualitatively coincide with the corresponding experimental values.
authors: O B
authors: OB
authors: Siverina
authors: E L
authors: EL
authors: Saenko
authors: V V
authors: VV
authors: Basevich
authors: A I
authors: AI
authors: Iaropolov
mesh_terms: D002570:Ceruloplasmin / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D010084:Oxidation-Reduction
chemical_list: D005296:Ferrous Compounds; D002570:Ceruloplasmin
vernacular_title: Izuchenie predstartsionnoĭ kinetiki ferrooksidaznoĭ reaktsii, kataliziruemoĭ tseruloplazminom.