title: [RNA-ligase of bacteriophage T4. IV. Synthesis of pentadecaribonucleotide ApUpG(pU)6(pA)5pAp].
abstract: Oligoribonucleotide ApUpG(pU)6(pA)5pAp (I) has been prepared by means of RNA ligase. ApUpG, synthesised by the phosphotriester approach, was elongated in the 3'-direction by adding (pU)6 and then (pA)6, which was 3'-blocked with the phosphate or with the periodate-oxidized AMP residue, the latter giving considerably lower level of by-products. Condensation of ApUpG(pU)6 with blocked (pA)6 was carried out under conditions optimal for poor acceptors (20 degrees C, 48 h, pH 8,7) to afford (I) with the yield of 20% (105 OE260); ApUpG(pU)6(pA)10pAp was identified as a byproduct.
authors: V V
authors: VV
authors: Antsiferova
authors: A G
authors: AG
authors: Ven'iaminova
authors: M N
authors: MN
authors: Repkova
authors: V I
authors: VI
authors: Iamkovoĭ
mesh_terms: D002384:Catalysis; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D011118:Polynucleotide Ligases; D011131:Polyribonucleotides / Q000138:chemical synthesis*; D011130:RNA Ligase (ATP); D013604:T-Phages / Q000201:enzymology*
chemical_list: D009843:Oligoribonucleotides; D011131:Polyribonucleotides; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)
vernacular_title: RNK-ligaza bakteriofaga T4. IV. Sintez pentadekaribonukleotida ApUpG(pU)6(pA)5pAp.