Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels: application to ethidium bromide-stained DNA.
We have built an electronic imaging system based on a modified charge-coupled-device television camera that directly quantitates the distribution of fluorescence from electrophoretic gels, chromatograms, and other stationary sources. Exposure times can exceed 1 min. Unlike the photographic system that it replaces, the response of the camera is directly proportional to the intensity of incident fluorescence, and image data are digitized and stored in computer memory ready for analysis immediately upon completion of an exposure. We describe procedures for the display, normalization, and archival storage of image data and programs that use images of ethidium bromide-stained DNA in alkaline agarose gels to quantitate single-strand breaks in DNA.
Sutherland
J C
JC
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
Lin
B
B
Monteleone
D C
DC
Mugavero
J
J
Sutherland
B M
BM
Trunk
J
J
D004247:DNA / Q000032:analysis*; D004581:Electronics / Q000295:instrumentation; D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*
D004247:DNA; D004996:Ethidium