title: The reaction of acetaldehyde with brain microtubular proteins: formation of stable adducts and inhibition of polymerization.
abstract: Stable adducts were formed by treatment of bovine brain microtubular proteins (MTP) with acetaldehyde, followed by gel filtration to remove excess acetaldehyde. The extent of stable adduct formation was determined using [14C]acetaldehyde and was correlated with acetaldehyde concentration and reaction time. Significant inhibition of MTP polymerization was observed at adduct concentrations of 0.6 mol acetaldehyde/mol tubulin dimer. The data suggest that repeated exposure of MTP to low concentrations of acetaldehyde, as would occur in the brain and other tissues of alcoholics, may inhibit MTP polymerization with neurological consequences.
authors: Department of Biochemistry, University of Queensland, St. Lucia, Australia.
authors: G
authors: G
authors: McKinnon
authors: J
authors: J
authors: de Jersey
authors: B
authors: B
authors: Shanley
authors: L
authors: L
authors: Ward
mesh_terms: D000079:Acetaldehyde / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D006160:Guanosine Triphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008868:Microtubule Proteins / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism
chemical_list: D008868:Microtubule Proteins; D011108:Polymers; D006160:Guanosine Triphosphate; D000079:Acetaldehyde