title: Inactivation of Bacillus piliformis spores by heat and certain chemical disinfectants.
abstract: The inactivation of Tyzzer's organism (Bacillus piliformis) spore isolated from rats by heat and various chemical disinfectants was studied. The spores were from B. piliformis-infected rat liver tissues. The spore suspension (10(4) 50% of rat liver lesion producing dose with prednisolone treatment/ml) was treated with heart or disinfectants. Inactivation of the spores was examined in experimentally infected rats. Rats were inoculated perorally with a treated spore suspension and injected subcutaneously with prednisolone. On the sixth day after inoculation, rats were examined grossly for liver lesions. Spores were inactivated at 80 degrees C for 15 min but not at 60 degrees C for 30 min. Spores were inactivated by 0.4% peracetic acid, 0.015% sodium hypochrolite, 1% iodophol, 5% phenol. Alcide and 0.37% formaldehyde solution, but not by 0.037% formaldehyde solution, 70% ethanol, 0.3% benzethonium chloride solution, 3% cresol and soap solution, or 4% chlorhexidine digluconate. These findings suggest that B. piliformis spores are relatively sensitive to heat and certain chemical disinfectants.
authors: Central Institute for Experimental Animals, Kawasaki, Japan.
authors: T
authors: T
authors: Itoh
authors: M
authors: M
authors: Ebukuro
authors: N
authors: N
authors: Kagiyama
mesh_terms: D000818:Animals; D001407:Bacillus / Q000187:drug effects / Q000502:physiology*; D001424:Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D004202:Disinfectants / Q000494:pharmacology*; D006358:Hot Temperature; D012376:Rodent Diseases / Q000382:microbiology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology
chemical_list: D004202:Disinfectants; D012973:Sodium Hypochlorite