Electrochemical detection of benperidol in serum for drug monitoring in humans.
A high-pressure liquid chromatographic (HPLC) method for the serum assay of benperidol is described. One ml of serum is required for a single estimation. The method involves a simple and rapid extraction step (BondElut columns), HPLC separation (C8 10-mu column), and electrochemical detection (+0.65 V). Haloperidol is used as internal standard. On the basis of this procedure, recovery (93-97%) and reproducibility (intra-assay and inter-assay coefficients of variation less than 9%) are satisfactory. The detection limit is 0.2 ng/ml of serum. After therapeutic doses, trough serum levels ranged from 3.8 to 12 ng/ml in five patients.
Furlanut
M
M
Department of Pharmacology, University of Padua, Italy.
Perosa
A
A
Benetello
P
P
Colombo
G
G
D001544:Benperidol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008991:Monitoring, Physiologic
D001544:Benperidol