Determination of organic acids in sugar cane process juice by high-performance liquid chromatography: improved resolution using dual Aminex HPX-87H cation-exchange columns equilibrated to different temperatures.
The application of high-performance liquid chromatography to the quantitative analysis of organic acids in sugar cane process juice is described. Separation of these acids is achieved on Aminex HPX-87H cation-exchange columns. Resolution is improved by connecting two columns in series and equilibrating them at different temperatures. The acids in the sample are first isolated on DEAE-Sephadex A-25 anion-exchange resin equilibrated with 0.5 M isobutyric acid. They are then eluted with 0.5 M sulphuric acid and injected directly onto the HPLC column after filtration through a 0.45-micron membrane. This method allows the simultaneous determination of oxalic, cis-aconitic, citric, phosphoric, malic, trans-aconitic, succinic, glycolic, lactic, formic and acetic acids. Isobutyric acid is also separated on this system and provides a useful guide to the loading capacity of the anion-exchange resin.
Blake
J D
JD
Sugar Research Institute, Mackay, Queensland, Australia.
Clarke
M L
ML
Richards
G N
GN
D000143:Acids / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005525:Food-Processing Industry; D007475:Ion Exchange Resins; D013395:Sucrose / Q000032:analysis*; D013696:Temperature
D000143:Acids; D007475:Ion Exchange Resins; D013395:Sucrose