Mitogenic activity in platelet-poor plasma from rats with persistent liver nodules or liver cancer.
Platelet-poor plasma (PPP) from F-344 rats with chemically-induced preneoplastic liver nodules or hepatocellular carcinoma stimulated S-phase DNA synthesis in monolayer cultures of normal rat hepatocytes. Similar mitogenic activity was detected in PPP 6 hrs to 1 week after partial hepatectomy (PH) or after necrotizing doses of CCl4 or diethylnitrosamine (DENA). Very little activity was found in PPP4 from control rats. The mitogenic activity in PPP from animals with nodules was non-dialyzable (greater than 14 kd) and bound to a heparin-sepharose affinity column. None of the mitogenic PPPs competed with [125I] epidermal growth factor (EGF) for binding sites on A431 cells or normal rat hepatocytes. These studies indicate that persistent proliferation of preneoplastic and neoplastic hepatocytes is associated with increased circulating levels of mitogenic hepatocyte growth factor.
Semple
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Department of Pathology, University of Toronto, Ontario, Canada.
Hayes
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MA
Rushmore
T H
TH
Harris
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Farber
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D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D002478:Cells, Cultured; D004261:DNA Replication; D066246:ErbB Receptors / Q000378:metabolism; D007399:Interphase; D007700:Kinetics; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000097:blood*; D008297:Male; D011230:Precancerous Conditions / Q000097:blood*; D051381:Rats; D011916:Rats, Inbred F344
D066246:ErbB Receptors