PSL, a nuclear cell-cycle associated antigen is increased during retinoic acid-induced differentiation of HL-60 cells.
PSL(p55) is a nuclear 55kD antigen present in various mammalian cell systems, which has been first identified by use of human autoimmune antibodies (Barque et al. 1983, EMBO J. 2, 743). It has been shown to be associated with interphase chromatine and to be synthesized in during the S phase of the cell cycle. In this work, we have analysed the status of PSL in promyelocytic HL-60 human cells in exponential or stationary growth, or undergoing granulocytic differentiation in presence of Retinoic acid. By use of 2-dimensional electrophoresis, PSL was found to be composed of two acidic proteins designated p55A and p55B. Unexpectedly, estimated 10-20 fold higher amounts of each species were found in cells treated for 5 days with 10(-6)M Retinoic acid, than in asynchronously growing cells or resting cells. Moreover, the p55A protein was phosphorylated during the process. On the basis of these results, PSL appears to be involved in some steps of the granulocytic differentiation process.
Barque
J P
JP
Unité 301 INSERM, Hôpital St-Louis, Paris, France.
Lagaye
S
S
Ladoux
A
A
Della Valle
V
V
Abita
J P
JP
Larsen
C J
CJ
D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D009687:Nuclear Proteins; D010750:Phosphoproteins; C044854:nuclear antigen p55, human; D014212:Tretinoin