Modulation of collagen synthesis by fat-storing cells, isolated from CCl4- or vitamin A-treated rats.
In an attempt to elucidate the role of fat-storing cells (FSCs) in liver fibrosis, we investigated the collagen synthesis by FSCs freshly isolated from rats treated with CCl4, with vitamin A, and from untreated rats. FSCs from CCl4-treated rats contained a small number of lipid droplets and an abundant rough endoplasmic reticulum (RER), while those from vitamin A-treated rats showed numerous large lipid droplets and scanty RER. The population doubling times of FSCs isolated from normal, CCl4-treated, and vitamin A-treated rats were 38 +/- 4.3, 24 +/- 2.5, and 48 +/- 6.3 hr, respectively. The rate of collagen synthesis by FSCs from CCl4-treated rats was four- to sixfold enhanced, while collagen synthesis by FSCs from vitamin A-treated rats was suppressed. The ratio of collagen type I to type III produced by FSCs from CCl4 rats was enhanced as compared with control rats (94.7:5.3 vs 87.6:12.4). Therefore, FSCs can be considered to play an important role in the pathogenesis of liver fibrosis.
Shiratori
Y
Y
Laboratory for Cell Biology and Histology, Faculty of Medicine and Pharmacy, Free University of Brussels, Belgium.
Ichida
T
T
Geerts
A
A
Wisse
E
E
D000818:Animals; D002251:Carbon Tetrachloride / Q000009:adverse effects*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D004224:Diterpenes; D004721:Endoplasmic Reticulum / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology
D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate; D003094:Collagen; D002251:Carbon Tetrachloride