Involvement of thiol-disulfide groups in the sensitivity of fully grown mouse oocytes to calcium-free medium.
The lethality caused by calcium-free medium (CFM) to fully grown mouse oocytes significantly decreases if a disulfide reducing agent (dithiothreitol, reduced glutathione, or L-cysteine) is added to the medium. In this condition, most of the surviving oocytes do not spontaneously resume meiosis. We also show that the sulfhydryl content of fully grown oocytes, estimated by monobromobimane labeling, rapidly decreases during culture in CFM. The hypothesis is discussed that lethality of oocytes cultured in CFM may be a consequence of an alteration of thiol-disulfide balance.
De Felici
M
M
Department of Anatomy and Cell Biology, II University of Rome, Italy.
Dolci
S
S
Siracusa
G
G
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004220:Disulfides / Q000378:metabolism*; D005260:Female; D008540:Meiosis; D051379:Mice; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D004220:Disulfides; D013438:Sulfhydryl Compounds; D002118:Calcium