[Effects of environmental changes around tumor cells on immunogenicity, and its mechanisms].
I studied alterations in the tumorigenicity and immunogenicity of cultured rat fibrosarcoma clone in different growth environments. When the fetal calf serum concentration in a medium was lowered from 10% to 1%, the tumorigenicity was diminished while the immunogenicity was enhanced, accompanied by a prolongation of the in vitro doubling time in a reversible manner. These phenomena were not associated with the quantities of the tumor-associated antigen and/or the rat major histocompatibility complex, but were associated with the appearance of a unique antigen (s), which consist of glycoprotein and exist as a crypt-antigen (s). Our observations indicate that the in vitro growth environment modifies the surface of tumor cells and causes a reduction in their tumorigenicity and an enhancement of their immunogenicity.
Chiba
I
I
First Department of Oral Surgery, Hokkaido University, School of Dentistry, Sapporo, Japan.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002453:Cell Cycle; D002999:Clone Cells; D003470:Culture Media; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000276:immunology* / Q000473:pathology
D000951:Antigens, Neoplasm; D003470:Culture Media