Determination of glucose turnover in sea bass Dicentrarchus labrax. Comparative aspects of glucose utilization.
1. Parameters of in vivo glucose utilization by sea bass (132 +/- 6 g, mean +/- SEM) acclimated at 15 degrees C in sea-water were measured after single injection of labelled glucose. 2. Glucose turnover rate (RG; mumol . min-1 . kg-1) was found to be 0.55-065 (2-3H glucose) and 0.34 +/- 0.42 (U14C glucose). 3. Glucose transit time was 443-449 min, glucose mass 233-261 mumol . kg-1, and glucose recycling 37%. 4. Oxygen consumption (MO2) amounted to 94 +/- 6.2 mumol . min-1 . kg-1. 5. The comparison with other fish species, mammals and birds, taking into account body size, temperature, diet, exercise, in poikilotherms and homeotherms leads to the calculation of a glucose turnover index (RGI = RG x 6 x 100 x MO2(-1)). 6. Value of this, generally lower in ectotherm teleosts (2-9), than in endotherms: mammals, birds and thunidae (22-60), confirms the minor quantitative importance of glucose in the metabolism of most fish.
Garin
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Laboratoire de Physiologie générale et comparée, Université Claude Bernard-Lyon, Villeurbanne.
Rombaut
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Fréminet
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A
D000818:Animals; D001492:Bass / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002645:Chickens; D005215:Fasting; D005399:Fishes / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D010473:Perciformes / Q000378:metabolism*; D005082:Physical Exertion; D051381:Rats; D013045:Species Specificity; D013217:Starvation; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D005947:Glucose