Determination of metoclopramide and its glucuronide and sulphate conjugates in human biological fluids (plasma, urine and bile) by ion-pair high-performance liquid chromatography.
Metoclopramide was determined in human biological fluids (plasma, urine and bile) by reversed-phase ion-pair high-performance liquid chromatography using a newly introduced cyanopropyl column. The method is precise, selective and sensitive: the mean recoveries of metoclopramide from plasma, urine and bile were 74.4, 99.1 and 85.9%, respectively; the mean within- and between-run coefficients of variation were, respectively, 0.8 and 8.5% for plasma and 2.0 and 8.2% for urine at the drug concentration of 100 ng/ml, and 2.3 and 11.2% for bile at the concentration of 20 ng/ml; the lower detection limit was 2 ng/ml for 1 ml of each biological fluid. Enzymic hydrolysis of a urine or bile specimen was used in the identification of metoclopramide, as well as its glucuronide and sulphate conjugates, from the human samples. A preliminary study on metoclopramide determinations from plasma and urine samples of a healthy subject and from bile samples of a patient demonstrated the clinical applicability of the method for therapeutic monitoring and pharmacokinetic studies.
Takahashi
H
H
Department of Biopharmaceutics, Meiji College of Pharmacy, Tokyo, Japan.
Ogata
H
H
Echizen
H
H
Ishizaki
T
T
D001646:Bile / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D008787:Metoclopramide / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D013464:Sulfuric Acids / Q000032:analysis
D005965:Glucuronates; D007202:Indicators and Reagents; D013464:Sulfuric Acids; D008787:Metoclopramide