Lectins as probes for the assay of rhabdovirus infections in plants.
Thirteen different, biotinylated plant lectins were tested for their ability to recognize specifically the glycoproteins of the two different plant rhabdoviruses potato yellow dwarf virus and eggplant mottled dwarf virus. All viruses were propagated on the same plant host species, Nicotiana rustica L. The lectin-binding to the viral proteins was tested after electrophoretic separation and transfer to nitrocellulose membranes. Besides purified virus also partially pure virus preparations were used for the tests, in order to determine the specificity. The lectins had been selected for specificities to either one of the following monosaccharides: mannose, glucose, galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine and fucose. In the test panel of thirteen lectins, seven were found to react with the viral glycoproteins. Among these, four (LCA, VFA, PSA, Con A) belonged to the mannosyl- or glycosyl-specific group. However, these four lectins reacted also with other host proteins when partially pure virus preparations were used as samples. The other three lectins (GSA2b, STA, WGA) were specific for N-acetyl-D-glucosamine and detected almost exclusively the viral glycoproteins. Two of these lectins, STA and WGA, were extremely suitable for virus-specific assays, since they did not react with glycoproteins in healthy controls that were identical or comparable in their electrophoretic mobility with the rhabdovirus glycoproteins. No binding to viral glycoproteins was observed with galactose-, N-acetyl-galactosamine- and fucose-specific lectins. The assay for rhabdovirus glycoproteins in plants with the lectins was approximately 8-16 times less sensitive than with virus-specific antibodies.
Adam
G
G
Institute for Biology, University of Stuttgart, F.R.G.
Heegard
P
P
Bøg-Hansen
T C
TC
Mundry
K W
KW
D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins; D037121:Plant Lectins; D010944:Plants / Q000382:microbiology*; D012209:Rhabdoviridae / Q000032:analysis / Q000302:isolation & purification*; D014764:Viral Proteins / Q000032:analysis*
D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D014764:Viral Proteins