Sealed vesicles prepared by fusing Mycoplasma gallisepticum membranes and preformed lipid vesicles.
Sealed vesicles were obtained by fusing Mycoplasma gallisepticum membranes with asolectin-cholesterol vesicles. The fusion was induced by freezing and thawing followed by a brief sonication treatment and was detected using a fluorescence sedimentation behavior in a sucrose density gradient and were shown to be impermeable to small solutes. The advantages of such fused preparations for transport studies in mycoplasmas are discussed.
Cirillo
V P
VP
Department of Biochemistry, State University of New York at Stony Brook.
Katzenell
A
A
Rottem
S
S
D001692:Biological Transport; D002462:Cell Membrane; D002499:Centrifugation, Density Gradient; D002784:Cholesterol; D005453:Fluorescence; D008081:Liposomes; D008561:Membrane Fusion; D009174:Mycoplasma / Q000032:analysis*; D010713:Phosphatidylcholines; D010743:Phospholipids
D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; C024990:asolectin; D002784:Cholesterol