[Determination of urinary oxalate using a Sigma kit].
Many methods for measuring urinary oxalate have been reported. However, most of them are unsuitable for clinical use because of complexity and difficulty. We tested the value of the Sigma kit for measuring urinary oxalate. In principle, after extraction from urine, oxalate is oxidized to hydrogen peroxide in the presence of oxalate oxidase and the hydrogen peroxide reacts with 3-methyl-2 benzothiozolinone hydrazone and 3-(dimethylamino) benzoic acid in the presence of peroxidase to yield an indamine dye with a maximum absorbance at 590 nm. The linearity of the standard curve and reproducibility of this method were measured and good results were obtained. (Linearity: r = 0.998, within assay: C.V. = 1.5 to 5.0%, between assays: C.V. = 4.0 to 11.6%). The correlation between this method and modified Hodgkinson and Williams' method or ion chromatographic method were 0.922 and 0.796, respectively. The overestimation effect of added ascorbic acid was completely inhibited by FeCl3 which had been added beforehand.
Suzuki
K
K
Department of Urology, Kanazawa Medical University.
Domiki
C
C
D000328:Adult; D006801:Humans; D008297:Male; D008722:Methods; D010070:Oxalates / Q000652:urine*; D019815:Oxalic Acid; D011933:Reagent Kits, Diagnostic; D012016:Reference Values
D010070:Oxalates; D011933:Reagent Kits, Diagnostic; D019815:Oxalic Acid