Radioimmunoassay used to detect DNA alkylation adducts in tissues from populations at high risk for oesophageal and stomach cancer.
N-Nitrosamines are alkylating agents capable of forming various covalent adducts with DNA in vivo. Since formation of promutagenic DNA adducts, particularly O6-methyldeoxyguanosine (O6-medGuo), has been implicated as an initiating event in nitrosamine-induced carcinogenesis, we have used radioimmunoassay (RIA) to probe for such damage in surgical tissue samples from cancer patients in populations likely to be exposed environmentally to nitrosamines.
Wild
C P
CP
International Agency for Research on Cancer, Lyon, France.
Lu
S H
SH
Montesano
R
R
D000478:Alkylation; D002681:China; D004247:DNA / Q000032:analysis* / Q000187:drug effects; D004938:Esophageal Neoplasms / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D008297:Male; D009602:Nitrosamines / Q000009:adverse effects; D011863:Radioimmunoassay / Q000379:methods; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics*
D009602:Nitrosamines; D006147:Guanine; D004247:DNA; C008449:O-(6)-methylguanine