[A method of isoelectric focusing in a borate-polyol system with pH gradient of 4.0 to 5.6 in a thin layer of polyacrylamide gel].
A procedure is described for thin-layer polyacrylamide gel isoelectric focusing involving borate-polyol system with gradient pH 4.0-5.6, where 0.5 M H3BO3-Tris was used as an electrode buffer. Riboflavin, TEMED, Tris-borate buffer and glycerol were used in preparation of gradient pH, which was stable and did not alter during the electrophoresis. Blood serum proteins, particularly blood serum albumin, from patients with diabetes were studied using the isoelectric focusing procedure. After isoelectrofocusing of blood serum albumin from patients with diabetes additional minor acid fractions of the protein were detected at pH 4.3-4.6. The procedure of electrophoresis in gradient pH 4.0-5.6 was highly effective and might be used in biochemical laboratories.
Azhitskiĭ
G Iu
GIu
Zagorul'ko
G V
GV
Troitskiĭ
G V
GV
D001798:Blood Proteins / Q000032:analysis; D001881:Borates; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D011108:Polymers
D001798:Blood Proteins; D001881:Borates; D011108:Polymers; C024617:polyol
Metodika izoélektricheskogo fokusirovaniia v borat-poliol'noĭ sisteme v gradiente pH ot 4,0 do 5,6 v tonkom cloe PAAG.