Ethylene glycol-stabilized haemolysates for glycated haemoglobin assay. Further analytical investigations and applications to quality control programmes.
The direct effect of the ethylene glycol on the quantitation of glycated haemoglobins by ion-exchange minicolumn chromatography, affinity chromatography and high performance liquid chromatography was investigated. No evident effect was found for ethylene glycol concentrations between 0.0 and 0.5 (volume fraction). The ethylene glycol-stabilised haemolysates were also analysed by HPLC: the elution profiles were similar to those of haemolysates fresly prepared from whole blood, and they remained unchanged, even after 18 months of storage at -20 degrees C. A preliminary inter-laboratory trial among 15 Italian laboratories was organized, using these ethylene glycol-stabilised haemolysates and, for comparison, a lyophilized commercial preparation. From the data obtained in this trial we confirmed the validity of using ethylene glycol-stabilized haemolysates as control materials, both for intra- and inter-laboratory standardization of glycated haemoglobin measurements.
Mosca
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Dipartimento di Scienze e Tecnologie Biomediche, Universita' degli Studi di Milano, Italy.
Carpinelli
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Bonini
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PA
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D005026:Ethylene Glycols; D006442:Glycated Hemoglobin / Q000032:analysis*; D006461:Hemolysis; D006801:Humans; D011786:Quality Control
D005026:Ethylene Glycols; D006442:Glycated Hemoglobin