title: On the choice of laser dyes for use in exciting tyrosine fluorescence decays.
abstract: The choice of laser dyes for exciting tyrosine fluorescence using synchronously pumped cavity-dumped dye laser systems is discussed. Rhodamine 560 was found to be optimal for a system based on an argon-ion pumping laser, whereas rhodamine 575 was preferred using a frequency-doubled Nd:YAG laser. Modifications of our fluorescence decay instrument to permit rejection of multiphoton events using a microchannel plate photomultiplier are described. An example of a four-component resolution of tyrosine decays illustrates the dramatic resolution capabilities attainable.
authors: Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
authors: L J
authors: LJ
authors: Libertini
authors: E W
authors: EW
authors: Small
mesh_terms: D007834:Lasers; D012235:Rhodamines; D013050:Spectrometry, Fluorescence; D014443:Tyrosine; D014966:Xanthenes
chemical_list: D012235:Rhodamines; D014966:Xanthenes; D014443:Tyrosine