Use of salicylate to estimate the "threshold" inducer level for de novo synthesis of the phenol-degrading enzymes in Pseudomonas putida strain H.
A special approach was used to elucidate the "threshold" inducer concentration for coordinative de novo synthesis of phenol hydroxylase(s), catechol 2,3-dioxygenase and the 2-hydroxymuconic semialdehyde-metabolizing enzymes which initiate phenol catabolism in Pseudomonas putida strain H. It is based on cell-precultivation with glucose (as the carbon and energy source) in the presence of different concentrations of sodium salicylate which proved to be a potent non-metabolizable inducer in strain H of these enzymes. Subsequent estimation of the activity status of resting cell suspensions and cell-free extracts, respectively, prepared from those strain H cultures clearly revealed failing de novo synthesis of the mentioned phenol-degrading enzymes at salicylate concentrations lower than 0.2 mg/l.
Janke
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Akademie der Wissenschaften der DDR.
D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D004790:Enzyme Induction; D006867:Hydrolases / Q000096:biosynthesis*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D010105:Oxygenases / Q000096:biosynthesis*; D019800:Phenol; D010636:Phenols / Q000378:metabolism; D011506:Proteins; D011549:Pseudomonas / Q000201:enzymology*; D012980:Sodium Salicylate / Q000378:metabolism*
D010636:Phenols; D011506:Proteins; D019800:Phenol; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; C022336:phenol 2-monooxygenase; D006867:Hydrolases; C092541:2-hydroxymuconate-semialdehyde hydrolase; D012980:Sodium Salicylate