The laboratory identification of serum antinuclear antibody in the dog.
An indirect immunofluorescence test with sections of rat liver as a substrate, proved useful in detecting antinuclear antibodies (ANA) in the dog. A specific anti-canine IgG reagent conjugated with fluorescein isothiocyanate was used. A proportion of normal dogs gave positive reactions at low titres. The presence of ANA was one of the criteria used to diagnose canine systemic lupus erythematosus. Some dogs within a general hospital population were also positive for the auto-antibody. The indirect immunofluorescence test with Trypanosoma brucei as a substrate was insensitive. The LE-cell test was laborious and insensitive. A commercial latex slide agglutination test used for detecting ANA in man gave false positive and false negative results in the dog. A commercial radioimmunoassay used in man gave many false positive results. A similar assay with synthetic DNA gave encouraging results and is worthy of further study.
Bennett
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Department of Veterinary Clinical Science, University of Liverpool Small Animal Hospital, U.K.
Kirkham
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D000818:Animals; D001168:Arthritis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000662:veterinary*; D002467:Cell Nucleus / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology*; D004285:Dogs; D005260:Female; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D008297:Male; D012016:Reference Values
D001323:Autoantibodies