title: The bioartificial kidney: progress towards an ultrafiltration device with renal epithelial cells processing.
abstract: The combination of an ultrafiltration device with an exchanger whose semipermeable hollow fibres are covered with renal epithelial cells is proposed as a design for a bioartificial kidney. We first demonstrated that continuous ultrafiltration can be maintained for relatively long periods in the absence of anticoagulation. As a second step, we report here the feasibility of attaching and growing two lines of kidney epithelial cells (MDCK and LLC-PK1) on two different semipermeable materials, an acrylic copolymer and a polysulphone. Cells seeded on acrylic copolymer hollow fibres reach confluence within three weeks. Depending on the chemical and/or physical properties of the polymer, the cells show distinct differentiated morphology, which may influence their ability to perform specialized tasks.
authors: Artificial Organ Laboratory, Brown University, Providence, RI 02912.
authors: P
authors: P
authors: Aebischer
authors: T K
authors: TK
authors: Ip
authors: G
authors: G
authors: Panol
authors: P M
authors: PM
authors: Galletti
mesh_terms: D001705:Bioprosthesis; D004847:Epithelial Cells; D004867:Equipment Design; D006440:Hemofiltration / Q000295:instrumentation*; D006801:Humans; D007668:Kidney / Q000166:cytology*; D007688:Kidneys, Artificial