A rapid separation method for inositol phosphates and their isomers.
A technique is described using ACCELL QMA anion-exchange SEP-PAKs (Waters Associates) with ammonium formate-based solutions, whereby a sample can be processed within minutes to yield resolution of inositol phosphates. Isomers of inositol trisphosphate can then be separated by using this technique in combination with a rapid (5-6 min) isocratic h.p.l.c. procedure. The use of QMA SEP-PAKs offers a degree of reproducibility comparable with that of h.p.l.c. while maintaining the capacity for automation, allowing large numbers of samples to be processed rapidly.
Wreggett
K A
KA
Department of Biochemistry, Agricultural and Food Research Council, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Irvine
R F
RF
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods*; D007295:Inositol Phosphates / Q000302:isolation & purification*; D007536:Isomerism; D013403:Sugar Phosphates / Q000302:isolation & purification*
D007295:Inositol Phosphates; D013403:Sugar Phosphates