title: A human cell-surface glycoprotein that carries Cromer-related blood group antigens on erythrocytes and is also expressed on leucocytes and platelets.
abstract: A new human erythrocyte glycoprotein has been identified by immunoblotting with murine monoclonal antibodies under non-reducing conditions. The glycoprotein has a MW of 70,000 and carries Cromer-related blood group antigens. The monoclonal antibodies also react with normal peripheral blood leucocytes and platelets and several haemopoietic cell lines. The glycoprotein has a reduced MW after sialidase treatment. The MW is markedly reduced in Tn erythrocyte membranes and slightly increased in Cad erythrocyte membranes. These results suggest that the glycoprotein has a substantial content of O-glycans. The glycoprotein appears to be absent from, or grossly altered in, the erythrocytes of two individuals with the rare Inab phenotype.
authors: Western Regional Blood Transfusion Centre, Southmead, Bristol, London, U.K.
authors: F A
authors: FA
authors: Spring
authors: P A
authors: PA
authors: Judson
authors: G L
authors: GL
authors: Daniels
authors: S F
authors: SF
authors: Parsons
authors: G
authors: G
authors: Mallinson
authors: D J
authors: DJ
authors: Anstee
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis*; D001789:Blood Group Antigens / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008970:Molecular Weight; D010641:Phenotype
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001789:Blood Group Antigens; D006023:Glycoproteins