Glycosylated prolactin in the murine pituitary: detection by a novel assay and alteration of concentrations by physiological and pharmacological stimuli.
In both rat and mouse pituitary extracts, we detected concanavalin A-binding prolactin immunoreactivity by a lectin-binding radioimmunoassay developed recently. The activity increased in response to estradiol benzoate treatment and lactation, stimuli that augment prolactin secretion, and decreased in response to acute nursing and perphenzine administration, stimuli that cause massive release of prolactin. Western blot analysis revealed a prolactin-immunoreactive band 2,000-3,000 greater in Mr than the main prolactin band that bound to 125I-labeled concanavalin A. These results suggest the existence in the murine adenohypophysis of a glycosylated form of prolactin, which seems to be released under certain physiological states.
Sinha
Y N
YN
Lutcher Brown Department of Biochemistry, Whittier Institute for Diabetes and Endocrinology, Scripps Memorial Hospital, La Jolla, CA 92037.
Jacobsen
B P
BP
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007118:Immunoassay / Q000379:methods; D007774:Lactation; D037102:Lectins; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010052:Ovariectomy; D010546:Perphenazine / Q000494:pharmacology*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D011388:Prolactin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000494:pharmacology
D037102:Lectins; C043928:prolactin, glycosylated; D001971:Bromocriptine; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D010546:Perphenazine