Fibrinolysis by urokinase endowed with magnetic property.
The activated magnetic modifier was synthesized from magnetite, alpha, omega-dicarboxymethylpoly(oxyethylene) and N-hydroxysuccinimide (Biochem. Biophys. Res. Commun., 145, 908-914, 1987). Urokinase was directly coupled with the activated magnetic modifier to obtain magnetic urokinase. The magnetic urokinase dispersed in saline and exerted high fibrinolytic activity (13.8 X 10(4) IU/mg protein), and was readily recovered from saline by magnetic force of 250 Oe. By applying magnetic force, the urokinase was attracted at our will and local fibrinolysis was achieved on fibrin gel in a petri dish.
Inada
Y
Y
Laboratory of Biological Chemistry, Tokyo Institute of Technology, Japan.
Ohwada
K
K
Yoshimoto
T
T
Kojima
S
S
Takahashi
K
K
Kodera
Y
Y
Matsushima
A
A
Saito
Y
Y
D005342:Fibrinolysis; D006801:Humans; D007700:Kinetics; D008280:Magnetics; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism* / Q000652:urine
D014568:Urokinase-Type Plasminogen Activator