title: Specificity of the amidase and kininogenase methods for the determination of rat urinary kallikrein.
abstract: The specificity of the amidase and kininogenase methods for determining rat urinary kallikrein was studied. Male and female rat urine was employed. Esterase A1, A2 and kallikrein were separated by DEAE-Sephadex A-50 chromatography. Esterase A1 showed no amidase activity towards the substrate H-D-Val-Leu-Arg-p-nitroanilide. In contrast, esterase A2 and kallikrein attacked the substrate, and the activity of kallikrein was especially inhibited by aprotinin, while esterase A2 was more sensitive to soybean trypsin inhibitor. Esterase A1 did not show kininogenase activity, whereas esterase A2 showed this activity, but only towards the dog plasma substrate. Kallikrein possessed kininogenase activity towards both dog and rat plasma kininogen. We believe that the most specific method for measuring rat urinary kallikrein activity is the kininogenase method using partially purified rat plasma kininogen.
authors: Laboratorio de Fisiología, Facultad de Ciencias Biológicas Pontificia Universidad Católica de Chile.
authors: V M
authors: VM
authors: Berthoud
authors: J L
authors: JL
authors: Corthorn
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000581:Amidohydrolases / Q000037:antagonists & inhibitors; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004950:Esterases / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000652:urine*; D007705:Kinins / Q000032:analysis; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007705:Kinins; D004950:Esterases; D007610:Kallikreins; D000581:Amidohydrolases