The dendrites of single brain-stem motoneurons intracellularly labelled with horseradish peroxidase in the cat. Morphological and electrical differences.
The geometrical differences between individual dendrites of a given motoneuron were investigated in the cat. We chose two brain-stem motoneurons involved in different motor activities. One abducens and one laryngeal motoneuron were selected from two series of experiments which had combined intracellular recording and horseradish peroxidase staining. Three-dimensional reconstructions were made using a computer-aided microscope to obtain high-resolution measurements from serial histological sections. Each dendrite was characterized by computer dissection. Comparisons between dendrites were made on the basis of the following parameters: spatial projections, length, diameters, tapering, branching pattern, daughter--branch ratio and branching power. The present findings show that each dendrite projects to specific terminal fields for both motoneurons and are different in the complexity of their geometry and branching structure. The consequences of this complexity for the cable properties of the motoneurons were analysed. The dendrites of the two motoneurons were partitioned into a series of contiguous regions deemed short enough to be considered an isopotential cylinder and the steady-state properties were calculated for each segment. The properties of each segment were then combined for each dendrite for the following parameters: electronic distance, somatopetal and somatofugal voltage attenuation, input resistance and charge transfer effectiveness ratio. The present results show significant differences in the electrical behaviour of individual dendrites. Branch-to-branch computation reveals low attenuation pathways between branches suggesting the possibility of local influences within the distal branches of the dendritic arborization. It is proposed that the individual dendrites of the motoneuron function as distinct channels and/or integrators for afferent inputs.
Bras
H
H
INSERM-U 6, CNRS-UA 634, Marseille, France.
Gogan
P
P
Tyc-Dumont
S
S
D000010:Abducens Nerve / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D001933:Brain Stem / Q000502:physiology* / Q000648:ultrastructure; D002415:Cats; D003198:Computer Simulation; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D007091:Image Processing, Computer-Assisted; D007823:Laryngeal Nerves / Q000502:physiology / Q000648:ultrastructure; D008564:Membrane Potentials; D009046:Motor Neurons / Q000502:physiology* / Q000648:ultrastructure; D009431:Neural Conduction
D006735:Horseradish Peroxidase