Fluorescence-detected circular dichroism studies of serum albumins.
Multidimensional fluorescence-detected circular dichroism (FDCD) is used to investigate the binding of bilirubin to four mammalian serum albumins. It is shown that the complexes formed with the albumins have distinctly different FDCD spectra. The effect of the pH-dependent transition from basic to acidic conditions on the complexes is examined. The binding of warfarin to albumin is also presented to demonstrate the general analytical utility of the multidimensional FDCD measurement for biochemical analysis.
Thomas
M P
MP
Department of Chemistry, Emory University, Atlanta, Georgia 30322.
Patonay
G
G
Warner
I M
IM
D000818:Animals; D001663:Bilirubin; D002942:Circular Dichroism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D012709:Serum Albumin; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
D012709:Serum Albumin; D001663:Bilirubin