Analysis of ceramide and monohexaosyl glycolipid derivatives by high-performance liquid chromatography and its application to the determination of the molecular species in tissues.
Several molecular species of monohexaosyl glycolipids and ceramides were analyzed as their benzoyl derivatives by high-performance liquid chromatography on a silica column with mixtures of dioxane and hexane as eluents. Monohexaosyl glycolipid derivatives were classified according to their hydrophobic structures, and ceramides were separated based on the fatty acid groups and the long-chain bases. The method allowed us to observe the heterogeneities in the molecular species of cerebrosides and ceramides from the epidermis and dermis of the guinea pig, and clear differences in the molecular species were observed between the different regions.
Snada
S
S
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
Uchida
Y
Y
Anraku
Y
Y
Izawa
A
A
Iwamori
M
M
Nagai
Y
Y
D000818:Animals; D002518:Ceramides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000032:analysis*; D006168:Guinea Pigs; D008055:Lipids / Q000032:analysis; D012867:Skin / Q000032:analysis
D002518:Ceramides; D006017:Glycolipids; D008055:Lipids