Dermal penetration of carbofuran in young and adult Fischer 344 rats.
Dermal penetration of carbofuran was determined in young (33 d) and adult (82 d) female Fischer 344 rats employing in vivo and in vitro methods. In vivo dermal penetration at 120 h was 43% for young and 18% for adult rats. The half-time for carbofuran skin penetration (in vivo) was 128 h for the young and 400 h for the adults. The young to adult ratio of dermal penetration was greater than 1 at all time points (average 2.9) and had a maximum of 4.2 at 24 h. Cumulative urinary excretion approached about 95% of the absorbed dose in both the young and adult animals at 120 h. Whole-body retention was slightly higher in adults. Kidney showed the highest tissue-to-blood concentration ratio (4.6 in adult, 2.3 in young). The ratio for the carcass was 2.8 in the adult and 2.4 in the young. The urine/blood concentration ratio was high, 435 in the adult and 573 in the young. The feces/blood ratio was 44 in the adult and 65 in the young. Skin absorption by the in vitro continuous-flow system was 41% for the young and 11% for the adult at 72 h, compared to 36% and 13% by the in vivo method. The static in vitro method gave consistently lower skin penetration values of 12% for the young and 8.8% for the adult. Differences in the kinetics of retention and excretion were observed between the young and adult animals.
Shah
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Northrop Services, Inc., Research Triangle Park, North Carolina.
Fisher
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Sumler
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Hall
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D000287:Administration, Topical; D000367:Age Factors; D000818:Animals; D002235:Carbofuran / Q000008:administration & dosage* / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D007306:Insecticides / Q000008:administration & dosage*; D008657:Metabolic Clearance Rate; D010539:Permeability; D051381:Rats; D011916:Rats, Inbred F344; D012867:Skin / Q000378:metabolism*; D014018:Tissue Distribution
D007306:Insecticides; D002235:Carbofuran