[Characteristics of [3H]cycloheximide distribution, protein and DNA biosynthesis in rat organs after sublethal blocking of translation].
The kinetics of accumulation and release of [3H]cycloheximide (CHI) as well as protein and DNA biosyntheses in some organs of the rats injected with sublethal doses of CHI were studied. It was shown that in the majority of organs under study (especially in the liver, kidneys and adrenals) the inhibition is completed within 12 hours after CHI injection followed by the resumption of protein and DNA syntheses. In the thymus and pancreas the levels of these biosyntheses remain below control values up to the 72nd hour. A positive correlation was observed between the decrease of CHI (or its metabolites) concentration and the beginning of protein and DNA syntheses in different organs. However, there was a reverse correlation between the high values of squares below the kinetic curves of CHI release from the liver, kidneys and adrenals and the intensive resumption of protein and DNA biosyntheses in these organs. It was thus assumed that in these particular organs CHI is subjected to intensive biotransformations. The contribution of the endocrine system to the induction of intensive compensatory protein and DNA syntheses in the liver were estimated from the viewpoint of the nature of reconstructive processes occurring in the appropriate organs.
Mitrokhin
Iu I
IuI
Gutnikova
M N
MN
Boĭkov
P Ia
PIa
Chirkov
G P
GP
Shevchenko
N A
NA
D000818:Animals; D001711:Biotransformation; D003513:Cycloheximide / Q000378:metabolism* / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats; D014018:Tissue Distribution
D004247:DNA; D003513:Cycloheximide
Osobennosti raspredeleniia [3H]tsiklogeksimida, biosintez belka i DNK v organakh krysy dosle subletal'nogo bloka transliatsii.