Castanospermine blocks the hyperglycemic response to carbohydrates in vivo: a result of intestinal disaccharidase inhibition.
Castanospermine is a potent inhibitor of rat intestinal glycohydrolases in vitro and prevents the hyperglycemic response to an oral sucrose challenge in vivo. Among the glycohydrolases tested, castanospermine was most effective against sucrase with an IC50 of 1.1 x 10(-7) M. In vivo, a significant effect was seen at doses less than 1 mg/kg in both normal and streptozotocin-treated rats. Castanospermine has a prolonged duration of activity in vivo with significant activity when administered 4 hours before sucrose.
Rhinehart
B L
BL
Merrell Dow Research Institute, Cincinnati, OH 45215.
Robinson
K M
KM
Payne
A J
AJ
Wheatley
M E
ME
Fisher
J L
JL
Liu
P S
PS
Cheng
W
W
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004186:Disaccharidases / Q000037:antagonists & inhibitors*; D007212:Indolizines; D007422:Intestines / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D054836:Indolizidines
D000470:Alkaloids; D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004186:Disaccharidases; D007212:Indolizines; C037806:castanospermine; D054836:Indolizidines