Erythropoiesis in murine long-term bone-marrow cell cultures: dependence on erythropoietin and endogenous production of an erythropoietic stimulating activity.
Erythropoiesis was obtained in murine long-term bone-marrow cell cultures (LTBMCs) in the presence of erythropoietin (Epo) when the medium was frequently renewed. The level of the erythropoietic differentiation was shown to be a function of the erythropoietin concentration. In response to Epo addition, an activity which stimulates CFU-E proliferation in semisolid cultures of fresh bone marrow cells was detected in the LTBMC supernatants. These results suggest that another factor, whose synthesis may be under Epo control, participates in the stimulation of erythropoiesis in vitro.
Oddos
T
T
Laboratoire de Biologie Cellulaire, Unité Associée au CNRS no92 Université Claude Bernard Lyon, Villeurbanne, France.
Nacol-Lizard
S
S
Blanchet
J P
JP
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004920:Erythropoiesis; D004921:Erythropoietin / Q000502:physiology*; D005260:Female; D007700:Kinetics; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019714:Tissue Inhibitor of Metalloproteinases
D003470:Culture Media; D008222:Lymphokines; D019714:Tissue Inhibitor of Metalloproteinases; D004921:Erythropoietin