Separation by high-performance liquid chromatography of penicillins with C4 to C10 aliphatic side chains.
A suitable and rapid method for the simultaneous determination of different aliphatic penicillins is described. Butyryl-, pentanoyl-, hexanoyl-, heptanoyl-, octanoyl-, nonanoyl- and decanoylpenicillin can be completely separated by high-performance liquid chromatography using an isocratic elution mode (50 mM H2KPO4, pH 5.0:methanol, 45:55 v/v). Under these conditions, retention times for C4 to C10 aliphatic side-chain penicillins were 2.5, 2.8, 4.1, 5.8, 8.9, 15.3, and 28.2 min. The benzylpenicillin retention time was 3.3 min.
Luengo
J M
JM
Antibioticos S.A., Leon, Spain.
Moreno
M A
MA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005227:Fatty Acids; D010406:Penicillins / Q000302:isolation & purification*
D005227:Fatty Acids; D010406:Penicillins