The mechanism of liposomal damage by taurocholate.
The stability of small unilamellar vesicles formed by egg-yolk phosphatidylcholine (PC) has been examined in the presence of sodium taurocholate. The permeability of the vesicular membrane changes as the total taurocholate concentration increases, until a transformation from mixed bile salt/PC vesicles to mixed micelles occurs. Based on experiments in which the bile salt-induced release of either hydrophilic (carboxyfluorescein) or hydrophobic (Bromothymol blue) probes was studied, and on fluorescence polarization of the probe 1,6-diphenyl-1,3,5-hexatriene and turbidity measurements, a two-step process for the initial stage of liposomal damage by taurocholate is postulated.
Walde
P
P
Department of Industrial Chemistry, Faculty of Engineering, Nagasaki University, Japan.
Sunamoto
J
J
O'Connor
C J
CJ
D001647:Bile Acids and Salts / Q000494:pharmacology; D001979:Bromthymol Blue; D004530:Egg Yolk; D005452:Fluoresceins; D005454:Fluorescence Polarization; D008081:Liposomes; D009391:Nephelometry and Turbidimetry; D010713:Phosphatidylcholines; D013656:Taurocholic Acid / Q000494:pharmacology*
D001647:Bile Acids and Salts; D005452:Fluoresceins; D008081:Liposomes; D010713:Phosphatidylcholines; C024098:6-carboxyfluorescein; D013656:Taurocholic Acid; D001979:Bromthymol Blue