Solid phase synthesis of oligoribonucleotides using the o-nitrobenzyl group for 2'-hydroxyl protection and H-phosphonate chemistry.
Oligoribonucleotides with chain length of 7, 11, 15, 17, 24 and 34 were synthesized on long chain alkylamine controlled pore glass beads (LCA-CPG) using o-nitrobenzyl protection of 2'-hydroxyls via a H-phosphonate approach either manually or by using an automatic synthesizer. The oligoribonucleotides were obtained in yields of 0.6 0.6-20%, based on initial nucleoside bound to the LCA-CPG support.
Tanaka
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Tamatsukuri
S
S
Ikehara
M
M
D001331:Automation; D001483:Base Sequence; D008722:Methods; D009843:Oligoribonucleotides / Q000138:chemical synthesis*
D009843:Oligoribonucleotides