Effect of heparin on membrane associated clathrin basketwork of cultured cells derived from the stromal-vascular fraction of mouse brown adipose tissue.
The effect of heparin treatment on clathrin basketwork of cell membranes was examined in cultured cells derived from the stromal-vascular fraction of neonatal mouse brown adipose tissue. The cytoplasmic surface of the plasma membrane of ruptured cells was examined with surface replicas. Heparin treatment significantly decreased the extent of clathrin basketwork associated with the membrane from 3.4 to 0.2%. The loss of clathrin basketwork suggests that heparin treatment of these cells affected intracellular sorting processes associated with endocytosis.
Amende
L M
LM
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Chernick
S S
SS
Reed
G F
GF
Blanchette-Mackie
E J
EJ
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure*; D002478:Cells, Cultured; D002966:Clathrin / Q000378:metabolism*; D005614:Freeze Fracturing; D006493:Heparin / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron
D002966:Clathrin; D006493:Heparin