Assays and activities of glycosidic enzymes in bovine peripheral blood leukocytes.
Methods are described for the quantitative measurement of N-acetyl-beta-D-glucosaminidase, alpha-mannosidase and beta-glucuronidase in peripheral blood leukocytes of the bovine. Enzyme kinetics and stability were determined. Activities of the glycosidases in polymorphonuclear leukocytes and mononuclear leukocytes were determined using the optimized assays. Polymorphonuclear leukocytes had greater activities of N-acetyl-beta-D-glucosaminidase and alpha-mannosidase, and similar levels of beta-glucuronidase, when compared to mononuclear leukocytes.
Hurley
W L
WL
Animal Sciences Dept., Univ. of Illinois, Urbana 61801.
D000118:Acetylglucosaminidase / Q000097:blood; D000818:Animals; D002417:Cattle / Q000097:blood*; D004247:DNA / Q000097:blood; D005260:Female; D005966:Glucuronidase / Q000097:blood; D006026:Glycoside Hydrolases / Q000097:blood*; D007962:Leukocytes / Q000201:enzymology*; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D008361:Mannosidases / Q000097:blood; D009504:Neutrophils / Q000201:enzymology; D043323:alpha-Mannosidase
D004247:DNA; D006026:Glycoside Hydrolases; D008361:Mannosidases; D043323:alpha-Mannosidase; D005966:Glucuronidase; D000118:Acetylglucosaminidase