title: Induced stabilization of the transmembrane potential of Drosophila cells by heat shock and periodic applications of chloramphenicol.
abstract: The transmembrane potential of Drosophila salivary gland cells is largely decreased (by 78% within 120 min) in response to the application of 5 mM-chloramphenicol (CAP), with an initial slope of 0.5 mV min-1. This depolarization is reversed immediately after the CAP concentration is reduced to 0.06 mM by step-wise dilution with normal medium. At the same concentration, thiamphenicol (TAP) induces only a small reversible depolarization by less than 30% within 120 min. These results are in agreement with the different effects of CAP and TAP on respiration and induction of the heat-shock genes, as known from previous data. The extent of induced membrane depolarization decreases with the number of repeated applications of CAP to the same cell, alternating with 75-min periods of recovery. Moreover, reduced sensitivity to CAP is also observed in cells recovering from a transient heat shock (30 min, 36 degrees C) 45 min prior to the addition of CAP. This phenomenon is inhibited by cycloheximide (0.1 mM), which suggests an involvement of heat-induced proteins in the stabilization of certain membrane functions.
authors: Institut für Genetik, Universität des Saarlandes, Saarbrücken, GFR.
authors: H J
authors: HJ
authors: Behnel
authors: G
authors: G
authors: Weckbart
mesh_terms: D000818:Animals; D002701:Chloramphenicol / Q000494:pharmacology*; D004330:Drosophila / Q000187:drug effects*; D006358:Hot Temperature; D008564:Membrane Potentials / Q000187:drug effects; D012469:Salivary Glands / Q000187:drug effects; D013839:Thiamphenicol / Q000494:pharmacology
chemical_list: D002701:Chloramphenicol; D013839:Thiamphenicol