Lipid composition of cultured B16 melanoma cell variants with different lung-colonizing potential.
Lipid components influence several cell surface properties that are critical in different stages of the metastatic process. In this study, we examined whether the different lung-colonizing potential of B16-F1 and B16-F10 melanoma cells could be related to a characteristic lipid profile. The lipid analyses, carried out on the same cell cultures used for the assay of lung-colonizing potential, revealed characteristics in the lipid composition of both B16-F1 and B16-F10 melanoma cells that are common to other systems of malignant cells: a high level of 18:1 associated with low proportions of polyunsaturated fatty acids in phospholipids, accumulation of ether-linked lipids and absence of complex gangliosides. The two B16 melanoma variants differed significantly only with respect to ether-linked lipids, due to a higher level of alkyl-PC in B16-F10 than in B16-F1.
Calorini
L
L
Istituto di Patologia Generale, Università degli Studi di Firenze, Italy.
Fallani
A
A
Tombaccini
D
D
Mugnai
G
G
Ruggieri
S
S
D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005732:Gangliosides / Q000032:analysis; D014644:Genetic Variation; D005989:Glycerides / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary*; D008546:Melanoma, Experimental / Q000032:analysis / Q000473:pathology*; D051379:Mice; D010743:Phospholipids / Q000032:analysis
D005227:Fatty Acids; D005732:Gangliosides; D005989:Glycerides; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids; D002784:Cholesterol