Determination of benzoylecgonine and cocaine in biologic fluids by automated gas chromatography.
A method is described for extraction of the cocaine metabolite benzoylecgonine, conversion to the butyl ester derivative and gas chromatographic analysis using packed or capillary columns. Using a capillary column, cocaine and benzoylecgonine may be determined simultaneously. The extraction scheme has been designed to facilitate processing of large numbers of samples generated in pharmacokinetic studies. Structural analogues, m-toluylecgonine and m-toluylecgonine methyl ester, are used as internal standards. Concentrations as low as 10 ng/ml in 1-ml samples of plasma or urine are readily determined. Between-run coefficients of variation were 1.01% for cocaine and 4.18% for benzoylecgonine for concentrations of 75 and 350 ng/ml, respectively.
Jacob
P
P
Medical Service, San Francisco General Hospital Medical Center, CA 94143.
Elias-Baker
B A
BA
Jones
R T
RT
Benowitz
N L
NL
D001322:Autoanalysis; D002849:Chromatography, Gas; D003042:Cocaine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007202:Indicators and Reagents
D007202:Indicators and Reagents; C005618:benzoylecgonine; D003042:Cocaine