Characterization of neutral glycosphingolipids from porcine erythrocyte membranes.
1. Six neutral GSL fractions were purified from porcine erythrocyte membranes. 2. They were identified to be LacCer (14% of total neutral GSLs), 2-hydroxy acid-rich and -poor Gb3Cer (3 and 7%, respectively) and Gb4Cer (71%) by means of NMR spectrometry. 3. Monohexosylceramides (5%) were composed of GlcCer and GalCer with near amount. 4. All these GSL classes contained a high concentration (more than 20% of total acids in each class) of 2-hydroxy fatty acids. 5. GalCer and GlcCer contained considerable amounts of C16- and C18-acids, and of C18-phytosphingosine, whereas C24-acids and C18-sphingosine were predominant in the other GSLs. 6. A minor GSL fraction (less than 1% of total neutral GSLs) which migrated more slowly than Gb5Cer on a thin layer plate and composed of several GSL components contained L-fucose.
Sako
F
F
Department of Home Economics, Hokkaido University of Education, Sapporo, Japan.
Gasa
S
S
Makita
A
A
D000818:Animals; D002855:Chromatography, Thin Layer; D004910:Erythrocyte Membrane / Q000032:analysis*; D005227:Fatty Acids / Q000097:blood; D006028:Glycosphingolipids / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013552:Swine
D005227:Fatty Acids; D006028:Glycosphingolipids