Sexing of human sperm by discontinuous Percoll density gradient and its clinical application.
A discontinuous Percoll density gradient was employed for the selective isolation of human X-bearing sperm. The fraction rich in X-bearing sperm that sedimented through eight steps of a Percoll density gradient was inseminated into healthy volunteers. Six couples achieved pregnancy and each delivered a female baby without abnormality. For further separation, a Percoll density gradient consisting of 12 steps gave a 94% purification of X-bearing sperm (contamination with Y-bearing sperm: 6.4 +/- 1.8%) with a mean recovery of 23.3 +/- 6.4% (n = 6).
Iizuka
R
R
Department of Obstetrics and Gynaecology, School of Medicine, Keio University, Tokyo, Japan.
Kaneko
S
S
Aoki
R
R
Kobayashi
T
T
D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D007315:Insemination, Artificial; D007317:Insemination, Artificial, Homologous; D008297:Male; D011247:Pregnancy; D012729:Sex Chromosome Aberrations / Q000517:prevention & control*; D012732:Sex Determination Analysis; D014960:X Chromosome