Effect of NH4+ ions on phi 29 DNA-protein p3 replication: formation of a complex between the terminal protein and the DNA polymerase.
Ammonium ions stimulated the formation of the phi diameter 29 protein p3-dAMP initiation complex by decreasing the Km value for dATP in a purified system containing the viral terminal protein p3, the viral DNA polymerase p2, and the phi 29 DNA-protein p3 complex as a template. In addition, NH4+ ions stimulated the amount of p3-dAMP complex elongation and increased by about twofold the rate of elongation. The stimulatory effect of NH4+ ions on in vitro phi 29 DNA replication is probably related to the formation of a stable complex between the terminal protein and the DNA polymerase, which was detected only in the presence of NH4+ ions.
Blanco
L
L
Centro de Biología Molecular, Universidad Autónoma de Madrid, Spain.
Prieto
I
I
Gutiérrez
J
J
Bernad
A
A
Lázaro
J M
JM
Hermoso
J M
JM
Salas
M
M
D000645:Ammonium Sulfate / Q000494:pharmacology*; D001435:Bacteriophages / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D007700:Kinetics; D013698:Templates, Genetic; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D003838:Deoxyadenine Nucleotides; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase; D000645:Ammonium Sulfate