Characterization of heterologous desensitization of rat reticulocyte adenylate cyclase system.
Treatment of rat reticulocytes with isoproterenol caused about 50, 25, and 25% decreases in beta-adrenergic agonist-, fluoride-, and guanine nucleotide-stimulated adenylate cyclase activities, respectively. The desensitization was also induced by dibutyryl adenosine 3',5'-cyclic monophosphate (cyclic AMP) and 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) prevented the isoproterenol-induced desensitization, suggesting the involvement of cyclic AMP in the desensitization. Time course studies revealed that the desensitization to NaF-AlCl3 occurred faster than that to isoproterenol. Furthermore, the rate of the resensitization to NaF-AlCl3 by removal of isoproterenol was also faster than that to isoproterenol. Thus, it is likely that both guanine nucleotide-binding stimulatory regulatory protein, Ns, and beta-adrenergic receptor are sequentially involved in both desensitization and resensitization of the adenylate cyclase system in rat reticulocytes to isoproterenol.
Yamashita
A
A
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Kurokawa
T
T
Dan'ura
T
T
Yanagiuchi
H
H
Ishibashi
S
S
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000262:Adenylyl Cyclases / Q000032:analysis*; D000535:Aluminum / Q000494:pharmacology; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002712:Chlorides / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D011494:Protein Kinases / Q000502:physiology; D051381:Rats; D012156:Reticulocytes / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology; D013997:Time Factors
D017607:Aluminum Compounds; D002712:Chlorides; D007546:Isoquinolines; D010879:Piperazines; D000077410:Aluminum Chloride; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D012969:Sodium Fluoride; D000535:Aluminum; D011494:Protein Kinases; D000262:Adenylyl Cyclases; D007545:Isoproterenol