High-performance liquid affinity chromatography of liver plasma membrane proteins.
Plasma membrane proteins from liver were analysed by concanavalin A affinity and immunoaffinity high-performance liquid chromatography. In the method, four peptide bands with apparent molecular weights of 140,000, 120,000, 80,000 and 60,000 could be isolated. In the second method, with two immobilized monoclonal antibodies, two corresponding antigens--the membrane proteins dipeptidyl-peptidase IV and GP 110--could be highly purified from plasma membrane extract with good yield in only one step.
Josić
D
D
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Dahlem, F.R.G.
Hofmann
W
W
Habermann
R
R
Becker
A
A
Reutter
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000032:analysis; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A; D007074:Immunoglobulin G / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008565:Membrane Proteins / Q000302:isolation & purification*; D051381:Rats
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D008565:Membrane Proteins; D003208:Concanavalin A