On the non-existence of a tryptic peptide with biological activity derived from mouse nerve growth factor.
Reverse-phase high-performance liquid chromatography was used to analyse the products of treatment of mouse nerve growth factor with cyanogen bromide followed by trypsin as described by Mercanti et al. All the biological activity was found to be due to incompletely cleaved starting material. Total digestion with trypsin led to complete loss of activity.
Springer
C J
CJ
Department of Chemistry, University College, London, U.K.
Vernon
C A
CA
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D013363:Submandibular Gland / Q000032:analysis; D014357:Trypsin / Q000378:metabolism*
D000596:Amino Acids; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D014357:Trypsin; D003488:Cyanogen Bromide