title: Longitudinal flow of endolymph measured by distribution of tetraethylammonium and choline in scala media.
abstract: Longitudinal endolymph flow rate in the guinea pig cochlea was measured by determining the rate of migration of extrinsic ions, tetraethylammonium chloride (TEA) or choline, with a potassium sensitive ion-selective microelectrode (ISM). Low concentrations of iontophoretically injected TEA were detected with the ISM at various distances from the injection electrode. The results were variable when the ISM was used to record spread of TEA from turn II to turn I and vice versa. However, consistent data were obtained when the TEA spread was measured at different electrode separations (0.2, 0.5, 0.7 mm) within turn II. Electrode locations were systematically exchanged without changing their distance, i.e. the ISM electrode was placed basally or apically with respect to the TEA electrode. Comparison of data with a model, which combines the bulk diffusion of TEA and the flow of endolymph, is consistent with a rate of endolymph flow in turn II of about 0.2 mm/min, apex to base. A similar value was also obtained with the iontophoretic injection of choline. The endolymph flow rate may be different in turn I as indicated by measurements of compound action potential (CAP) changes. However, the results of experiments when TEA spread is measured at large distances must be interpreted cautiously because TEA may enter cellular walls of the cochlear duct and alternative routes of transport may be involved.
authors: Dept. of Physiology, University of Western Australia, Nedlands.
authors: E
authors: E
authors: Syková
authors: J
authors: J
authors: Syka
authors: B M
authors: BM
authors: Johnstone
authors: G K
authors: GK
authors: Yates
mesh_terms: D000200:Action Potentials; D000818:Animals; D001692:Biological Transport; D002794:Choline / Q000493:pharmacokinetics*; D003051:Cochlea / Q000378:metabolism*; D003053:Cochlear Duct / Q000378:metabolism*; D004058:Diffusion; D004710:Endolymph / Q000378:metabolism*; D006168:Guinea Pigs; D007761:Labyrinthine Fluids / Q000378:metabolism*; D008839:Microelectrodes; D008962:Models, Theoretical; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000493:pharmacokinetics*
chemical_list: D013757:Tetraethylammonium Compounds; D019789:Tetraethylammonium; D002794:Choline