title: Degeneration and atrophy of the thymus of lethally irradiated dogs, rescued by transfusion of cryopreserved autologous blood leukocytes.
abstract: Dogs exposed to a fatal radiation dose of 12 Gy were rescued by transfusion of autologous blood leukocytes. A severe acute and long-lasting damage to the thymus was observed. The acute damage, as observed on the tenth day, consisted of a marked reduction in the number of lymphocytes, degeneration of Hassall's bodies, and hemorrhage. Long-term effects, observed several months after irradiation, were partial to total atrophy of the thymus. Regeneration, when it occurred, was limited to a few small isolated areas in which lymphopoiesis was supported by epithelial reticular cells. In contrast, the lymph nodes of all dogs had abundant cortical lymphopoiesis. The abundant hemopoiesis present in the marrow from the tenth day after irradiation until the end of the observation period should have provided sufficient circulating precursor cells to seed the thymus and regenerate the organ to the same extent as that observed in the other blood-forming organs. The impairment of lymphopoietic regeneration in the thymus seems to be due, therefore, to damage caused by irradiation on the specific stroma of the organ, which is not able to support such activity.
authors: Department of Clinical Physiology and Occupational Medicine, University of Ulm, Federal Republic of Germany.
authors: W
authors: W
authors: Calvo
authors: T M
authors: TM
authors: Fliedner
authors: E W
authors: EW
authors: Herbst
authors: E
authors: E
authors: Hügl
authors: B
authors: B
authors: Bödey
mesh_terms: D000818:Animals; D001284:Atrophy; D003114:Colony-Forming Units Assay; D004285:Dogs; D005260:Female; D005615:Freezing; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000528:radiation effects; D017708:Leukocyte Transfusion; D008297:Male; D012016:Reference Values; D013950:Thymus Gland / Q000166:cytology / Q000473:pathology / Q000528:radiation effects*; D014021:Tissue Preservation; D014182:Transplantation, Autologous