Subchronic oral toxicity of 4-chloro-alpha, alpha, alpha-trifluorotoluene in Sprague-Dawley rats.
The subchronic oral toxicity of 4-chloro-alpha, alpha, alpha-trifluorotoluene (CTT) was assessed in Sprague-Dawley rats. Four groups of six male and six female rats were treated daily for 28 days, by gavage, with doses of 0, 10, 100 and 1000 mg CTT/kg body weight using olive oil as a vehicle. No clinical signs were observed, other than salivation in the high-dose group in the last week. The males of this group showed a significant decrease in body-weight gain without a concurrent decrease in food consumption. In males, there were significant dose-dependent increases in blood cholesterol and triglycerides, suggestive of alterations in lipid metabolism. The females showed only a small dose-related increase in serum lactate dehydrogenase. Specific histological alterations were found in the males given 1000 mg/kg/day, namely hyaline droplet nephrosis, along with a significant increase in relative kidney weight, and an increase in lipid vacuoles in the adrenal cortex. Slight nephrosis was also observed in males given 100 mg/kg. Both male and female rats showed a significant increase in relative liver weight at a dose of 1000 mg CTT/kg. CTT appears to have a low subchronic oral toxicity. Neither pathological nor biochemical alterations were found at 10 mg/kg body weight/day and this can be defined as the no-observable-effect level (NOEL).
Macrì
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Istituto Superiore di Sanità Laboratorio di Tossicologia Comparata ed Ecotossicologia, Viale Regina Elena, Rome.
Ricciardi
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C
Stazi
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AV
Mantovani
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A
Vendramin Macrì
C
C
Piccioni
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Badellino
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E
Bianchi
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MP
Pepe
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M
Ceccanti
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M
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology; D008297:Male; D051381:Rats; D012472:Salivation / Q000187:drug effects; D014050:Toluene / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity
D014050:Toluene; C037723:4-chloro-alpha,alpha,alpha-trifluorotoluene