Studies on monoclonal antibodies against human platelets--a monoclonal antibody to human platelet glycoprotein I--SZ-2.
A monoclonal antibody, SZ-2, reacts specifically on human platelets and megakaryocytes. The platelets from 10 normal donors are bound to 15,200 +/- 4,100 SZ-2 molecules/platelet. The antigen recognized by SZ-2 is chymotrypsin-sensitive but neuraminidase-insensitive, and has been identified as glycoprotein Ib (GPIb) by an affinity chromatography technique. SZ-2 is different from other monoclonal antibodies to GPIb. It inhibits not only platelet aggregation induced by ristocetin, but also platelet aggregation induced by collagen (type I) and by PAF. SZ-2 also inhibits platelet serotonin and beta-thromboglobulin release in response to these stimuli.
Ruan
C G
CG
Thrombosis and Haemostasis Research Unit, Suzhou Medical College.
Xi
X D
XD
Du
X P
XP
Wan
H Y
HY
Wu
X
X
Li
P X
PX
Gu
J M
JM
D000911:Antibodies, Monoclonal / Q000502:physiology*; D003094:Collagen; D006801:Humans; D010972:Platelet Activating Factor; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D012310:Ristocetin
D000911:Antibodies, Monoclonal; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D012310:Ristocetin; D003094:Collagen