Beta-oxidized N-nitrosoalkylcarbamates as models for DNA alkylation by N-nitrosobis(2-oxopropyl)amine in Syrian hamsters.
A single dose of N-nitrosobis(2-oxopropyl)amine (NDOPA) can selectively induce pancreatic-duct adenocarcinomas in Syrian hamsters. Multiple doses or a higher single dose can induce tumours of the liver and other organs. Our earlier studies employing NDOPA systematically labelled with 14C in the three-carbon chain showed that hamster pancreatic DNA is almost exclusively methylated and that the sole source of the methyl group is the alpha carbon of NDOPA. Hamster liver DNA was equally methylated and alkylated by a three-carbon chain. Current studies using generally labelled tritiated NDOPA with a very high specific activity have shown that the three-carbon alkylation is 2-hydroxypropylation. We have identified two adducts isolated from hamster liver DNA, N7-(2-hydroxypropyl)-guanine and O6-(2-hydroxypropyl)guanine, which contain this group, and we have also isolated and identified N7-methylguanine and O6-methylguanine in DNA from hamster liver and pancreas. beta-Oxidized N-nitrosocarbamates, ethyl N-nitroso-2-oxopropylcarbamate (NOPC) and ethyl N-nitroso-2-hydroxypropylcarbamate (NHPC), are useful models for predicting the DNA adducts observed in vivo following NDOPA treatment. Base-catalysed decomposition of NOPC in the presence of exogenous DNA yields five methylated purines (N3-, N7- and O6-methylguanines and N1- and N3-methyladenines). NHPC, a model for N-nitrosamines containing the 2-hydroxypropyl group, reacts with guanosine to yield N7- and O6-(2-hydroxypropyl)guanines.(ABSTRACT TRUNCATED AT 250 WORDS)
Nagel
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Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, Nebraska.
Lewis
R
R
Fischer
M
M
Stansbury
K
K
Stepan
K
K
Lawson
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TA
D000230:Adenocarcinoma / Q000139:chemically induced; D000478:Alkylation; D000818:Animals; D002219:Carbamates / Q000633:toxicity; D002417:Cattle; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D006207:Half-Life; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008647:Mesocricetus; D008745:Methylation; D009602:Nitrosamines / Q000633:toxicity*; D010190:Pancreatic Neoplasms / Q000139:chemically induced; D013329:Structure-Activity Relationship
D002219:Carbamates; D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine; D004247:DNA