Effect of blood coagulation and platelet aggregation on perfusable capillaries and arterioles in ischemic and nonischemic myocardium.
The aim of this study was to determine the effect of blood coagulation and platelet aggregation on the perfusability of arterioles (19-50 micron) and capillaries in subepicardial and subendocardial ischemic and nonischemic myocardium of anesthetized open-chest rabbits. Fluorescein isothiocynate-dextran (MW 150,000) was injected intravenously to label perfusable myocardial microvessels of rabbits that were subjected to 60 min of coronary artery occlusion. Fluorescent microscopy was used to identify the perfusable vessels and an alkaline phosphatase stain was employed to locate the total microvasculature of the heart. Stereological principles were utilized to determine various morphometric parameters. About 25% of the capillaries were incapable of being perfused but virtually all arterioles were perfusable in occluded myocardium of the control group. Essentially all capillaries and arterioles were perfusable in nonoccluded myocardium. Collagen infusion produced a perfusion defect in 14% of the capillaries and arterioles in nonoccluded myocardium and in 33% of the capillaries and arterioles in occluded myocardium. Heparin, prostaglandin E1 (PGE1), or PGE1 + heparin did not prevent the perfusion defect in capillaries of occluded myocardium. It is concluded that while promotion of blood coagulation and platelet aggregation was able to produce microvessel obstruction, these hemostatic mechanisms were not primarily responsible for the capillary obstruction observed during myocardial ischemia in the rabbit heart.
Rosolowsky
M
M
Physiology and Biophysics Department, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.
Weiss
H R
HR
D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D001158:Arteries / Q000503:physiopathology*; D001160:Arterioles / Q000503:physiopathology*; D001777:Blood Coagulation; D002196:Capillaries / Q000187:drug effects / Q000503:physiopathology*; D003094:Collagen / Q000494:pharmacology; D003326:Coronary Circulation; D003327:Coronary Disease / Q000097:blood / Q000503:physiopathology*; D006439:Hemodynamics; D006493:Heparin / Q000494:pharmacology; D008833:Microcirculation; D008856:Microscopy, Fluorescence; D010477:Perfusion; D010974:Platelet Aggregation; D011817:Rabbits
D006493:Heparin; D003094:Collagen; D000527:Alprostadil