title: Partitioning behaviour of rat bone marrow cells in aqueous two-phase systems. Dependence of cell partition on the interfacial tension and electrical potential difference between the phases.
abstract: The partitioning of heterogeneous cell populations of rat bone marrow, in comparison with that of homogeneous populations of erythrocytes, has been studied in aqueous two-phase systems containing increasing concentrations of dextran-poly-(ethylene glycol) (to increase the interfacial tension) and/or decreasing phosphate concentrations (to decrease both the electrical potential difference between the phases and the interfacial tension). At any polymer and/or phosphate concentration tested the partition is lower for bone marrow cells, reflecting their lower surface charge and hydrophobicity.
authors: Departamento de Bioquimica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
authors: A I
authors: AI
authors: García-Pérez
authors: M N
authors: MN
authors: Recio
authors: P
authors: P
authors: Sancho
authors: J
authors: J
authors: Luque
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D004563:Electrochemistry; D004912:Erythrocytes / Q000166:cytology; D008297:Male; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains; D013500:Surface Tension
chemical_list: D011092:Polyethylene Glycols