Production of an HLA-DRw13 antibody in a DRw14 positive multiparous woman.
A lymphocytotoxic HLA-DR alloantiserum (Ma159) which reacts with DR7, DR5 and DRw13 positive lymphocytes is described. It was non-cytotoxic against 27 DRw14 positive subjects and was not absorbed by DRw14 positive cells. The antiserum was produced by pregnancy alone in a DR4, DRw14 positive woman whose husband possesses DR7 and DRw13. Absorption and titration studies suggested that the antiserum contains separate antibodies directed towards DR7, DRw13 and a determinant shared by DR5 and DRw13. The implications of finding a specific anti-DRw13 antiserum and its production in a donor positive for the alternative subdivision of DRw6 are discussed.
Darke
C
C
Blood Transfusion Centre, Cardiff, Wales, U.K.
Street
J
J
D000918:Antibody Specificity; D005260:Female; D006680:HLA Antigens / Q000032:analysis; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000145:classification / Q000276:immunology*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D010298:Parity; D010375:Pedigree
D006680:HLA Antigens; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C097337:HLA-DR13 antigen; C052238:HLA-DR14; D007518:Isoantibodies