Simultaneous measurement of DNA content and detection of surface antigens of cervicovaginal cells by flow cytometry.
A method is described by which the measurement of the DNA content and the light scatter and the detection of a cervical carcinoma-associated antigen (CCA) of squamous epithelial cells can be simultaneously accomplished by flow cytometry (FCM). Cervicovaginal cellular samples obtained from 30 patients were analyzed by this method. Cell populations with an abnormal DNA content or with the presence of CCA were detected in 20 samples, 18 of which contained dysplastic cells as detected by routine cytologic screening. The remaining ten cases, which were interpreted as cytologically normal by routine screening, were also interpreted as normal by FCM analysis.
Flint
A
A
Department of Pathology, University of Michigan Medical Center, Ann Arbor 48109-0054.
McCoy
J P
JP
Esch
T R
TR
Beckwith
A L
AL
Morley
G W
GW
D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002584:Cervix Uteri / Q000166:cytology* / Q000276:immunology; D004247:DNA / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D014621:Vagina / Q000166:cytology* / Q000276:immunology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D004247:DNA