Separation of hemagglutination-inhibiting immunoglobulin M antibody to rubella virus in human serum by high-performance liquid chromatography.
High-performance liquid chromatography was successfully used to separate hemagglutination-inhibiting immunoglobulin M (IgM) rubella virus antibody from IgG rubella virus antibody in human serum. The fractionation by high-performance liquid chromatography was as effective as sucrose density gradient centrifugation in separating IgM antibody from IgG antibody.
Kobayashi
N
N
Suzuki
M
M
Nakagawa
T
T
Matumoto
M
M
D000914:Antibodies, Viral / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M / Q000302:isolation & purification*; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M