Use of reflectance in soft laser densitometry in scanning opaque electrophoregrams. A high-resolution technique.
The present report describes a new approach in soft laser scanning densitometry performed on opaque electrophoregrams. The method employs a light reflectance system instead of absorption, which is commonly used. The data of this preliminary study were obtained by directly scanning a photograph of an electrophoregram exhibiting viral proteins separated by SDS-PAGE. Comparison of the two methods clearly demonstrate that laser reflectance offers finer resolution than laser absorbance when opaque materials are scanned.
Zeineh
R A
RA
Kyriakidis
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G
D003720:Densitometry / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007834:Lasers; D014764:Viral Proteins / Q000032:analysis*
D014764:Viral Proteins