In vivo effect of brain-derived neurotrophic factor on the survival of developing dorsal root ganglion cells.
Implantation of silastic membranes between neural tube and somites at somitic levels 20-24 in 30-somite-stage chick embryos results in separation of early migrated neural crest cells of the dorsal root ganglion (DRG) anlage from the neural tube and their death within a few hours [Kalcheim and Le Douarin, (1986) Dev. Biol., 116, 451-460]. The in vivo effects of brain-derived neutrotrophic factor (BNDF) on survival of HNK-1 immunoreactive DRG cells separated from the tube were examined by implantation of laminin-treated silastic membranes (controls) or BDNF/laminin-treated membranes. In the presence of BDNF/laminin-treated membranes, 20/25 grafted embryos fixed 10 h after implantation, contained many rescued cells on the operated side. In contrast, only a few rescued cells on the operated side. In contrast, only a few rescued cells were observed in sections on the operated in 2/11 embryos implanted with laminin-treated silastic membranes, and no rescued cells at all could be detected in embryos implanted with NGF/laminin-treated (seven embryos) or untreated silastic membranes (12 embryos). The data presented support the hypothesis that early survival and differentiation of neural crest-derived sensory cells depend on central nervous system-derived factor(s). Moreover, this is the first evidence for the in vivo activity of BDNF on survival of developing DRG cells.
Kalcheim
C
C
Institut d'Embryologie du CNRS et du Collège de France, Nogent-sur-Marne.
Barde
Y A
YA
Thoenen
H
H
Le Douarin
N M
NM
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D007797:Laminin / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D009432:Neural Crest / Q000502:physiology*; D012825:Silicon
D019208:Brain-Derived Neurotrophic Factor; D007797:Laminin; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012825:Silicon