Structure of a psoralen cross-linked DNA in solution by nuclear magnetic resonance.
One- and two-dimensional nuclear magnetic resonance (NMR) methods were used to determine a three-dimensional model of an eight-base-pair DNA fragment (d-GGGTACCC) cross-linked with psoralen in solution. Two-dimensional nuclear Overhauser effect experiments were used to assign the spectrum and estimate distances for 171 proton pairs in the cross-linked DNA. The NMR-derived model shows a 53 degree bend into the major groove that occurs primarily at the site of drug addition and a 56 degree unwinding that spans the eight-base-pair duplex.
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Department of Chemistry, University of California, Berkeley 94720.
Wemmer
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Kim
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D001482:Base Composition; D003432:Cross-Linking Reagents; D004247:DNA; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008730:Methoxsalen; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D013941:Thymine
D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D013941:Thymine; D008730:Methoxsalen