Protein turnover and 3-methylhistidine excretion in non-pregnant, pregnant and gestational diabetic women.
Protein turnover was studied in nine non-pregnant (NP) and eight pregnant (P) women. The data from two gestational diabetic (GDM) women are included for comparison. Pregnant women were studied at 30-36 weeks gestation. Whole-body protein turnover, synthesis and catabolism rates were measured using a single dose of 15N-glycine followed by measurement of enrichment of urinary ammonia during the next 10 h. P and NP women had similar rates of protein turnover (4.8 g protein/kg/d) and synthesis (3.8 g protein/kg/d). GDM women appeared to have considerably higher rates for both turnover (5.6 g protein/kg/d) and synthesis (4.7 g protein/kg/d). Normal pregnant women excreted significantly more urinary 3-methylhistidine (3MH) than did non-pregnant women (190 vs 149 mumole/d). Correlation between 3MH excretion and protein catabolism rate approached significance (P = 0.087) in the NP women, but was poorly correlated (P = 0.355) in the P women.
Department of Nutritional Sciences, University of California, Berkeley 94720.
W L
WL
Fitch
J C
JC
King
D000328:Adult; D000641:Ammonia / Q000652:urine; D005260:Female; D006639:Histidine / Q000031:analogs & derivatives*; D006801:Humans; D008762:Methylhistidines / Q000378:metabolism* / Q000652:urine; D011247:Pregnancy / Q000378:metabolism* / Q000652:urine; D011254:Pregnancy in Diabetics / Q000378:metabolism* / Q000652:urine; D011506:Proteins / Q000378:metabolism*
D008762:Methylhistidines; D011506:Proteins; D006639:Histidine; D000641:Ammonia