Methanol extract of grain dust shows complement fixing activity and other characteristics similar to tannic acid.
We have found several similarities between tannic acid and grain dust extract prepared with methanol. Both formed a precipitate with IgG, and these interactions were inhibited by albumin. In addition, both preparations fixed complement; this activity was heat stable and was removed by prior adsorption of the preparations with hide powder. Adsorption with polyvinyl polypyrrolidone reduced the complement-fixing activity of tannic acid but not that of the methanol grain dust extract. The similarities between tannic acid and the methanol grain dust extract are consistent with the presence of a tannin or tanninlike material in grain dust.
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D000818:Animals; D003168:Complement Fixation Tests; D004391:Dust / Q000009:adverse effects / Q000032:analysis*; D002523:Edible Grain / Q000009:adverse effects / Q000032:analysis*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000009:adverse effects / Q000032:analysis*; D007074:Immunoglobulin G / Q000378:metabolism; D000432:Methanol; D009784:Occupational Diseases / Q000209:etiology; D012140:Respiratory Tract Diseases / Q000209:etiology; D013634:Tannins / Q000032:analysis*
D004391:Dust; D047348:Hydrolyzable Tannins; D007074:Immunoglobulin G; D013634:Tannins; D000432:Methanol