Use of the heterologous ovum penetration test to predict the fertilising capacity of human spermatozoa.
The ability of human spermatozoa to penetrate zona-free hamster ova in a heterologous ovum penetration (HOP) test was compared with the fertilisation of human oocytes in 54 normospermic couples. Concordant results were seen in 45/54 (83%) cases. However, false-positive HOP test results occurred in 5/45 (11%) couples and false-negative results in 4/9 (44%) couples. The data suggest that a positive HOP test result is a good indication that fertilisation will occur, although a negative HOP test result does not necessarily mean that fertilisation will not take place.
Department of Obstetrics and Gynaecology, King's College Hospital, London, UK.
P L
PL
Matson
P
P
Vaid
J H
JH
Parsons
R
R
Goswamy
W P
WP
Collins
J P
JP
Pryor
D000818:Animals; D006224:Cricetinae; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida