A new rapid silver impregnation for neuronal bodies.
Frozen sections of avian and rat brains routinely fixed in 6% glutaraldehyde/2% paraformaldehyde and left in phosphate buffer for 1 month are cut at 15-20 microns and collected in distilled water. Sections are placed in ammoniacal silver solution for 30 s, rinsed in 100% acetone and developed in a reducing solution at 75 degrees C. Sections are toned in 1% gold chloride solution and fixed in 50% sodium thiosulfate. After washing, the sections are dehydrated, cleared and mounted in the usual way for light microscopy.
Tolivia
J
J
Department of Morphology and Cellular Biology, Faculty of Biology and Medicine, University of Oviedo, Spain.
Tolivia
D
D
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D002645:Chickens; D009474:Neurons / Q000166:cytology*; D051381:Rats; D012834:Silver
C000212:ammoniacal silver; D012834:Silver