Oncogene expression in differentiating F9 mouse embryonal carcinoma cells.
Differentiation of F9 mouse embryonal carcinoma cells in culture is accompanied by a decrease in growth rate and loss of tumorigenicity. Cells differentiating in monolayer culture (to parietal endoderm-type cells) or in aggregates (to visceral endoderm-type cells) show qualitatively similar changes in transcript levels from several c-oncogenes. In contrast with other studies with F9 cells, we find an early decrease in c-myb RNA but not in c-myc RNA. This and a later increase in c-src RNA may be associated with decreasing cell growth rate. Before differentiation, induction and maintenance of elevated c-abl RNA levels depend on the presence of retinoic acid in the medium. After differentiation c-abl RNA levels decline only partially when retinoic acid is removed. Increased RNA from c-fos is seen late in differentiation in monolayer cultures only, a change also seen with appearance of similar endoderm cell types in the developing mouse embryo.
CSIRO Division of Molecular Biology, New South Wales, Australia.
T J
TJ
Lockett
M J
MJ
Sleigh
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000032:analysis / Q000378:metabolism*; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation; D051379:Mice; D014411:Neoplastic Stem Cells / Q000032:analysis / Q000378:metabolism*; D009857:Oncogenes; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000378:metabolism*
D012334:RNA, Neoplasm