Alteration in rat liver microsomal membranes induced by acetaminophen.
Acetaminophen caused a reversible change in the fluorescence polarization of eosin maleimide and dansyl chloride labeled to rat liver microsomes, indicating that acetaminophen acts on microsomal membrane proteins. Acetaminophen in the presence of nicotinamide adenine dinucleotide phosphate (NADPH), brought about an irreversible change in the fluorescence polarization of the probes. Acetaminophen, with or without NADPH, was also found to change the fluorescence polarization of lipid-soluble fluorescent probes, 2- and 12-(9-anthroyloxy)stearic acid labeled to microsomes, indicating its action on microsomal membrane lipids.
Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.
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Araya
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Horie
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Hayashi
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Awazu
D000082:Acetaminophen / Q000494:pharmacology*; D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005454:Fluorescence Polarization; D008297:Male; D008566:Membranes / Q000187:drug effects; D008862:Microsomes, Liver / Q000187:drug effects*; D009249:NADP / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000082:Acetaminophen; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D000111:Acetylcysteine