In vitro effects of 3-acetyl-deoxynivalenol on the immune response of human peripheral blood lymphocytes.
The effects of 3-acetyl-deoxynivalenol (3-AcDON) on the in vitro mitogen responses and the antibody producing ability of human peripheral blood lymphocytes were evaluated. 3-AcDON inhibited the proliferative response to pokeweed mitogen and concanavalin A at a lower concentration (100 ng/ml) as compared to phytohemagglutinin (200 ng/ml). The antibody producing ability was inhibited by 3-AcDON concentrations of greater than or equal to 200 ng/ml. Higher concentrations of 3-AcDON (greater than or equal to 300 ng/ml) produced severe suppression of plaque forming cell response in vitro and reduced the total yield of lymphocytes without altering cell viability. The results of this study indicate that 3-AcDON produces immunosuppressive effects in a dose dependent manner in vitro.
Tomar
R S
RS
Blakley
B R
BR
Schiefer
H B
HB
DeCoteau
W E
WE
D000328:Adult; D000917:Antibody Formation / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology*; D014255:Trichothecenes / Q000494:pharmacology*
D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D012717:Sesquiterpenes; D014255:Trichothecenes; C028784:phytohemagglutinin-P; D003208:Concanavalin A; C043247:3-acetyldeoxynivalenol