Serum opsonic activity after pneumococcal vaccination in splenectomized individuals as measured by a nitroblue tetrazolium (NBT) reduction micromethod.
19 children who were splenectomized after traumatic rupture and 15 healthy siblings were vaccinated with a 14-valent pneumococcal vaccine (Pneumovax). The serum opsonic activity against types 3, 6A, 7F, 14, 19F and 23F was evaluated before the vaccination and after 2 and 12 months by a new micromethod based on nitroblue tetrazolium reduction of granulocytes. Before the vaccination, opsonic activity was found more often in the splenectomized than in the controls, 38 and 29%, respectively. However, patients and controls responded similarly to the vaccine. The serum opsonic activity changed from negative to positive after vaccination in 31% of the splenectomized, as well as the controls. The results indicate that the protection against pneumococcal infection after vaccination may not be adequate against all pneumococcal types in all patients.
T
T
Urban
T
T
Linné
B
B
Jakobsson
C
C
Jarstrand
D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage; D001769:Blood / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D009580:Nitroblue Tetrazolium; D009895:Opsonin Proteins / Q000276:immunology*; D022242:Pneumococcal Vaccines; D013156:Splenectomy; D013296:Streptococcus pneumoniae / Q000276:immunology*; D013778:Tetrazolium Salts; D014611:Vaccination
D000906:Antibodies; D001428:Bacterial Vaccines; D009895:Opsonin Proteins; D022242:Pneumococcal Vaccines; D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium