Strategies for enzyme isolation using dye-ligand and related adsorbents.
Approaches to the use of a large range of dye-ligand and similar adsorbents for protein isolation are described. The adsorbents behave in a similar fashion relative to each other with a variety of different applied protein mixtures, so a system of dividing the adsorbents into five groups according to their protein-binding ability has been adopted. In this way it becomes unnecessary to screen all dyes, and an ideal selection of dyes and conditions can quickly be achieved.
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Scopes
D000327:Adsorption; D002021:Buffers; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity; D004396:Coloring Agents; D004798:Enzymes / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008024:Ligands
D002021:Buffers; D004396:Coloring Agents; D004798:Enzymes; D007202:Indicators and Reagents; D008024:Ligands