Immune effector mechanisms involved in the control of parasitaemia in Trypanosoma brucei-infected wildebeest (Connochaetes taurinus).
The course of Trypanosoma brucei infection in the wildebeest (Connochaetes taurinus) was studied. A low but persistent parasitaemia developed in all five wildebeest following intravenous inoculation with 1 X 10(8) organisms of clone ILTat 2.1. Unlike cattle controls, however, the wildebeest did not develop anaemia. In both cattle and wildebeest, radioimmunoassay studies revealed a classical sequence of production of IgM, IgG1 and IgG2 antibodies which had the capacity to bind to the corresponding purified variable surface glycoprotein and to neutralize the infectivity of ILTat 2.1. Investigations into the interaction between post-infection sera, trypanosomes and freshly isolated peripheral blood leucocytes (PBL) of wildebeest and cattle showed that sera from the wildebeest had a higher capacity to induce adherence of trypanosomes to homologous PBL. The adherence and phagocytosis-inducing activity resided in the IgM fraction. Cross-testing of the antibodies and PBL revealed that wildebeest IgM antibodies induced high adherence indices when tested on cattle PBL. High adherence indices were also observed when cattle IgM antibodies were tested on PBL of wildebeest. It was concluded that the phagocytic system of the wildebeest was superior to that of cattle, that freshly prepared wildebeest PBL bear receptors for wildebeest as well as cattle IgM, and that cattle PBL bear a receptor for wildebeest IgM that would appear to be different from that for cattle IgM.
F R
FR
Rurangirwa
A J
AJ
Musoke
V M
VM
Nantulya
C
C
Nkonge
L
L
Njuguna
E Z
EZ
Mushi
L
L
Karstad
J
J
Grootenhuis
D000818:Animals; D000866:Antelopes / Q000276:immunology* / Q000469:parasitology; D001188:Artiodactyla / Q000276:immunology*; D001769:Blood / Q000469:parasitology; D002417:Cattle; D002448:Cell Adhesion; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008297:Male; D009500:Neutralization Tests; D014353:Trypanosomiasis, African / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D014354:Trypanosomiasis, Bovine / Q000276:immunology* / Q000469:parasitology
D007074:Immunoglobulin G; D007075:Immunoglobulin M