An automated enzymic micromethod for the measurement of fat in human milk.
Most (98%) of the fat in human milk is present as triglycerides. This paper describes the use of a clarification procedure that enables the level of human milk fat to be determined by measurement of glycerol released by enzymic hydrolysis of triglycerides. The method requires only 10-50 microliters milk, thus presenting a possible technique for work with small mammals, and is suitable for use with autoanalysers, permitting rapid sample throughput.
Lucas
A
A
Medical Research Council, Dunn Nutrition Unit, Cambridge, UK.
Hudson
G J
GJ
Simpson
P
P
Cole
T J
TJ
Baker
B A
BA
D001322:Autoanalysis / Q000379:methods; D005223:Fats / Q000032:analysis*; D005990:Glycerol / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D008049:Lipase; D008832:Microchemistry / Q000379:methods; D008895:Milk, Human / Q000032:analysis*; D014280:Triglycerides / Q000032:analysis*
D005223:Fats; D014280:Triglycerides; D008049:Lipase; D005990:Glycerol