Antibody-mediated targeting of liposomes to red cells in vivo.
Covalent attachment of anti-rat erythrocyte F(ab')2 to liposomes specifically enhanced their binding to rat erythrocytes in vivo and reduced their uptake by the liver. Furthermore, at least 20-30% of the cell-bound liposomes delivered their contents to the cells. Besides, the liposome binding did not affect the survival time of the target cells at least up to 3 h in the blood circulation. These results demonstrate for the first time that liposomes can be successfully targeted to cells other than liver cells in vivo.
A
A
Singhal
C M
CM
Gupta
D000327:Adsorption; D000818:Animals; D004705:Endocytosis; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism*; D007140:Immunoglobulin Fab Fragments; D007444:Inulin / Q000378:metabolism; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008561:Membrane Fusion; D051381:Rats; D013154:Spleen / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D014018:Tissue Distribution
D007140:Immunoglobulin Fab Fragments; D008081:Liposomes; D013395:Sucrose; D007444:Inulin