Bulk-preparation of hepatic binding protein.
A method for the extraction in the cold of hepatic binding protein using a novel combination of detergents, namely 1% Na urso deoxycholate and 5% Tween 80 in the extraction buffer, which allows a significantly higher recovery of lectin than 1% Triton X-100 is described. It is noteworthy that this lectin preparation can be maintained in solution for over a month and can be used in vitro and in vivo.
G P
GP
Pessina
A
A
Naldini
V
V
Bocci
D000818:Animals; D037263:Asialoglycoprotein Receptor; D002352:Carrier Proteins / Q000302:isolation & purification*; D002462:Cell Membrane / Q000032:analysis; D007700:Kinetics; D037102:Lectins / Q000302:isolation & purification / Q000502:physiology; D008099:Liver / Q000032:analysis*; D011136:Polysorbates / Q000494:pharmacology; D011817:Rabbits; D012995:Solubility; D013656:Taurocholic Acid / Q000494:pharmacology; D013696:Temperature; D014580:Ursodeoxycholic Acid / Q000494:pharmacology
D037263:Asialoglycoprotein Receptor; D002352:Carrier Proteins; D037102:Lectins; D011136:Polysorbates; C027122:hepatic sialoglycoprotein receptor; D013656:Taurocholic Acid; D014580:Ursodeoxycholic Acid