Comparative analysis of myocardial enzyme activities of the energy-supplying metabolism in patients with dilative cardiomyopathies and valve diseases.
We determined representative enzyme activities of glycogenolysis (glycogen phosphorylase) glycolysis (d-glyceraldehyde-3-phosphate dehydrogenase, GAPDH), beta oxidation of free fatty acids (1-3-hydroxyacyl CoA dehydrogenase, HADH), citric acid cycle (citrate synthase, CS), lactate fermentation (lactate dehydrogenase LDH), and creatine phosphate metabolism (creatine kinase, CK) in left ventricular samples of 36 patients to investigate if the metabolic capacities of the energy-supplying pathways are differently affected in different heart diseases. There were 17 patients with mitral valve diseases (MVD), 8 patients with aortic valve diseases (AVD), and 11 patients who suffered from dilative cardiomyopathies (DCM). The main metabolic characteristic on the level of enzymatic organization in patients with DCM was an increased ratio of GAPDH/HADH activities and a decreased ratio of HADH/CS activities compared to the valve-diseased patients. This result indicates that the capacity of glucose oxidation is enhanced at the expense of fatty acid metabolism in patients with DCM. Furthermore, we determined significantly lower myocardial CK activities in this group of patients, most probably reflecting a diminished content of myofibrils. Citrate synthase activity was lowest in patients with AVD. Although we cannot rule out that the impaired left ventricular function is in part responsible for the shift of the capacities of the energy-supplying metabolism in patients with DCM, we favor the assumption that it is a specific feature of this myocardial disease.
Klein
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Spaar
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Schlepple
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Wiegand
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Kreuzer
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D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D002311:Cardiomyopathy, Dilated / Q000201:enzymology*; D002952:Citric Acid Cycle; D003402:Creatine Kinase / Q000378:metabolism; D004734:Energy Metabolism; D004798:Enzymes / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006019:Glycolysis; D006349:Heart Valve Diseases / Q000201:enzymology*; D006352:Heart Ventricles / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008875:Middle Aged; D009206:Myocardium / Q000201:enzymology*; D010725:Phosphocreatine / Q000378:metabolism
D004798:Enzymes; D005227:Fatty Acids; D007527:Isoenzymes; D007773:Lactates; D010725:Phosphocreatine; D019344:Lactic Acid; D006003:Glycogen; D015094:3-Hydroxyacyl CoA Dehydrogenases; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase