Improved enzyme-linked immunosorbent assay for the detection of orbivirus antigens by treatment with sodium dodecyl sulfate.
Several methods have been developed for treating antigens, that reduce background or inactivate virus utilizing toxic reagents for the enzyme-linked immunosorbent assay (ELISA). In this study, orbivirus infected BHK-2 1 cells were treated with SDS for increased sensitivity in antigen detection by ELISA. The sensitivity of 1.0% SDS treated material increased eightfold.
Yale Arbovirus Research Unit, Yale University School of Medicine, New Haven, CT 06510.
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Oprandy
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D012044:Regression Analysis; D012087:Reoviridae / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate
D000956:Antigens, Viral; D012967:Sodium Dodecyl Sulfate