Immunoturbidimetric assay for the determination of microalbuminuria using the Hitachi analyser.
A sensitive and specific immunoturbidimetric method is described for the determination of low concentrations of urinary albumin using a Hitachi 704 or 705 analyser. The sensitivity was 1 mg/l and the precision attained was good (CV 15%, 7% and 6% for low, medium and higher albumin concentrations). The assay was used to determine urinary albumin excretion rates in healthy controls (less than 15 micrograms/min) and in type I diabetics. Since microalbuminuria (30-200 micrograms/min) seems to be a good predictor for the development of diabetic nephropathy and other late diabetic complications, this assay is suitable for the necessary screening and follow-up of diabetic nephropathy. In contrast to RIA methods, no radioactive tracers are needed.
Landgraf-Leurs
M M
MM
Medizinische Klinik Innenstadt der Universität München, FRG.
Modi
E
E
Horn
K
K
Landgraf
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000419:Albuminuria / Q000652:urine*; D003920:Diabetes Mellitus / Q000652:urine; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D011863:Radioimmunoassay; D013055:Spectrophotometry, Infrared
D007202:Indicators and Reagents