Reprograming of murine blastocoele formation.
The present study shows that there is communication between reaggregated asynchronous cleavage stage blastomeres that regulates blastocoele formation. Individual blastomeres from eight-cell murine embryos were transferred to empty zonae pellucidae, intact two-cell embryos, or enucleated two-cell embryos, and were examined over a period of 75 hours for development of cavitation. It was found that the isolated blastomeres cavitated concurrently with intact control eight-cell embryos, while intact control two-cell embryos cavitated 24 hours later. However, the embryos resulting from combining a two-cell embryo and a blastomere from an eight-cell embryo cavitated at a time in between the eight- and two-cell controls.
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D000818:Animals; D001757:Blastomeres / Q000502:physiology*; D002450:Cell Communication; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015044:Zona Pellucida