[A method of determining pyruvate kinase activity].
A method is suggested for determining the activity of pyruvate kinase (ATP: pyruvate-phosphotransferase). The method is based on evaluation of the amount of ATP formed in the course of phosphoenolpyruvic-to-pyruvic acid transformation in the presence of ADP and magnesium ions. The ATP amount in the reaction medium is determined by paper electrophoresis with subsequent spectrophotometry.
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D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002384:Catalysis; D004590:Electrophoresis, Paper; D009132:Muscles / Q000201:enzymology; D011770:Pyruvate Kinase / Q000032:analysis*; D051381:Rats
D000255:Adenosine Triphosphate; D011770:Pyruvate Kinase
Sposob opredeleniia aktivnosti piruvatkinazy.