In vivo evaluation of intracellular pH and high-energy phosphate metabolites during regional myocardial ischemia in cats using 31P nuclear magnetic resonance.
Phosphorus-31 nuclear magnetic resonance spectroscopy (31P NMR) was used to assess the temporal changes of high-energy phosphate metabolites in the region of acute myocardial ischemia of open-chest cats. Eight anesthetized cats were studied following ligation of the left anterior descending coronary artery. Creatine phosphate showed a 79 +/- 16% (mean +/- SD) reduction by 4 min after the onset of ischemia. Prominent qualitative reductions of the spectral peak of creatine phosphate occurred by 40 s after ischemia. Adenosine triphosphate measured under the beta spectral peak (beta-ATP) decreased 37 +/- 9% by 20-25 min after ligation of the left anterior descending coronary artery. These reductions developed more slowly and were of smaller magnitude than those of creatine phosphate. Intracellular pH decreased from 7.39 +/- 0.07 to 7.13 +/- 0.09 units by 40 s after ischemia. By 30 min, pH decreased to 6.07 +/- 0.40 units. The study shows, therefore, the temporal changes of high-energy phosphate metabolites during ischemia in localized regions of the myocardium of open-chest animals.
Stein
P D
PD
Goldstein
S
S
Sabbah
H N
HN
Liu
Z Q
ZQ
Helpern
J A
JA
Ewing
J R
JR
Lakier
J B
JB
Chopp
M
M
LaPenna
W F
WF
Welch
K M
KM
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002415:Cats; D003327:Coronary Disease / Q000175:diagnosis*; D005260:Female; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D010725:Phosphocreatine / Q000032:analysis*; D010758:Phosphorus; D013057:Spectrum Analysis; D013997:Time Factors
D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate