Monosaccharide transport by Eimeria tenella sporozoites.
Eimeria tenella sporozoites were incubated in the presence of 3 different [14C]-labeled sugars; D-glucose, 2-deoxy-D-glucose and 3-O-methyl-D-glucose. The initial velocity, Vi, of uptake of D-glucose and 2-deoxy-D-glucose was similar, 41 micrograms/10(10) sporozoites/min and 46 micrograms/10(10) sporozoites/min, respectively; whereas that for 3-O-methyl-D-glucose was significantly lower, 17 micrograms/10(10) sporozoites/min. Initial velocity studies also revealed that glucose uptake was a saturable event, with an apparent KT of 20 mM and an apparent Vmax of 312 micrograms/10(10) sporozoites/min. Uptake was unaffected by exogenous sodium levels or the presence of ouabain. However, 0.1 mM phloretin significantly inhibited glucose uptake. Thus, it would appear that E. tenella sporozoites possess a Na-independent, phloretin-sensitive, carrier-mediated monosaccharide-transport system.
Smith
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D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002463:Cell Membrane Permeability; D004539:Eimeria / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D009005:Monosaccharides / Q000378:metabolism*; D010693:Phloretin / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology
D009005:Monosaccharides; D012964:Sodium; D005947:Glucose; D010693:Phloretin