Structural characterization of cholylcoenzyme A:glycine-taurine N-acyltransferase and a covalent substrate intermediate.
Bile acid-CoA:glycine-taurine N-acyltransferase was found to catalyze a reaction in the absence of glycine or taurine in which the substrate cholyl-CoA is cleaved with the release of CoA and the formation of a covalently bound enzyme-cholate intermediate. This unstable intermediate was trapped by a rapid mixing and denaturation procedure. The denatured protein was digested with trypsin and the cholate-labeled tryptic peptide was isolated. This cholate-peptide is considered to originate from the active site region of the enzyme based on the following criteria: cholyl-CoA does not react with any of the 20 common amino acids, the hydrolysis of cholyl-CoA is known to occur on the enzyme, the lack of reaction of the enzyme with just cholate, and the fact that labeling is extensive even at low (substrate level) concentrations of cholyl-CoA. The isolated cholate-peptide was submitted to amino acid analysis. It contained 32 amino acid residues and was devoid of cysteine, methionine, and tyrosine. Amino acid analysis of the N-acyltransferase was conducted. The enzyme was also shown to possess a blocked N terminus.
B
B
Czuba
D A
DA
Vessey
D000217:Acyltransferases / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003065:Coenzyme A / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D011487:Protein Conformation; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D000217:Acyltransferases; C025118:cholyl CoA glycine-taurine N-acyltransferase; D014357:Trypsin; D003065:Coenzyme A