[Depolymerization of actin microfilaments in the I--Z--I brushes of transverse striated muscles by using DNAse I].
To release Z-discs of skeletal muscles myofibrils from actin microfilaments, I--Z--I-brushes (complexes of Z-discs and thin filaments) were treated with DNAse I-both in suspension and on electron microscopical grids. It was shown that such a treatment resulted in depolymerization of actin filaments. The preparations obtained were heterogeneous and contained I--Z--I-brushes with shorter actin filaments and single Z-discs. The structure of Z-discs released from actin filament remained intact. Therefore these preparations may be used in studies on regulation of actin microfilaments assembly.
Khaĭtlina
S Iu
SIu
Goncharova
E I
EI
Orlova
A A
AA
D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism*; D000818:Animals; D003599:Cytoskeleton / Q000187:drug effects*; D003850:Deoxyribonuclease I / Q000494:pharmacology*; D008854:Microscopy, Electron; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D011817:Rabbits
D000199:Actins; D003850:Deoxyribonuclease I
Depolimerizatsiia aktinovykh mikrofilamentov v I--Z--I-shchetkakh poperechnopolosatykh myshts s pomoshch'iu DNKazy I.