[Effect of various conditions on the stability of DNA-methylases from Mycobacterium smegmatis butyricum cells].
DNA-methylases from M. sm. butyricum, produced by means of isoelectrofocusing, were characterized by slow spontaneous variations in the activity with amplitude of 75-80% under conditions of storage in glycerol at -10 degrees. Effect of various conditions on stabilization of activity of adenine methylases MMbu4,2 and MMbu7,2 was studied. Alterations in the enzymatic activity were not found in presence of substances applied usually to stabilize the enzymes, blood serum albumin and cations of two valent metals as the values of the alterations occurred in the ranges of the enzyme activity variations.
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EV
Sharkova
I I
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Nikol'skaia
P
P
Shomodi
I
I
Fel'desh
S S
SS
Debov
D004248:DNA (Cytosine-5-)-Methyltransferases / Q000032:analysis* / Q000037:antagonists & inhibitors; D004355:Drug Stability; D004356:Drug Storage; D007525:Isoelectric Focusing; D009161:Mycobacterium / Q000201:enzymology*; D011487:Protein Conformation
D004248:DNA (Cytosine-5-)-Methyltransferases
Vliianie razlichnykh usloviĭ na stabil'nost' DNK-metilaz iz kletok Mycobacterium smegmatis butyricum.