[Effect of bile and bile acids on monoamine oxidase and acetylcholinesterase activity in the jejunum of rabbits, guinea pigs and swine].
The studies were carried out in vitro on the jejunum of 10 pigs, 10 rabbits and 10 guinea pigs. The activity monoaminooxidase (MAO) was determined manometrically in Warburg's apparatus, that of acetylcholinesterase (AChE) spectrophotometrically after Ellman's method modified by urek and Syrowatka. The obtained results were statistically analysed. It was shown that in the animals studied the bile acids exerted a considerable effect on the activity of enzymes metabolising neurohormones. Under the influence of bile the activity of monoaminooxidase was inhibited. Stimulating, but less distinctly, bile effected the activity of acetylocholinesterase. Significant differences in the action of bile on the activity of these enzymes were found in the jejunum of the animal species studied. The highest decrease in the activity of MAO under the influence of bile was found in the jejunum of the rabbit (by about 65%), a weker in the pig (by 37.5%) and guinea-pig (by 18.5%). However, the highest increase in the activity of AChE under the influence of bile occurred in the pigs (by about 21.6%), a weaker in the rabbit (by 16.4%) and guinea-pig (by about 11.5%). Among the bile acids, the strongest action on both the activity of MAO and AChE was exerted by lithocholic acid, desoxycholic, weaker by cholic and chenodesoxycholic acid. MAO activity under the influence of lithocholic acid decreased (in the pig by about 63%, rabbit by 55.7% and guinea pig by 34.8%). After incubation of the jejunum in desoxycholic acid a decrease in MAO activity occurred (in the rabbit by 43.8%, pig by about 39.4% and guinea-pig by 15.7%). AChE activity under the influence of lithocholic acid increased (in the pig on average by 62.2% rabbit by 21.8 and guinea-pig by about 10.2%). After incubation of the jejunum in desoxycholic acid an increase in the activity of acetylcholinesteras occurred (in the pig by about 13.5%, guinea-pig by 7.7% rabbit 7.3%).
Instytut Nauk Fizjologicznych AR w Lublinie.
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Radymska-Wawrzyniak
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Studziński
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000502:physiology*; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007583:Jejunum / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors; D011817:Rabbits; D013045:Species Specificity; D013552:Swine
D001647:Bile Acids and Salts; D002800:Cholinesterase Inhibitors; D008996:Monoamine Oxidase Inhibitors; D008995:Monoamine Oxidase; D000110:Acetylcholinesterase
Wpływ zółci i kwasów zółciowych na aktywność monoaminooksydazy i acetylocholinesterazy w jelicie czczym królika, świnki morskiej i świni.