Ca2+ transport and permeability in inside-out red cell membrane vesicles after freezing.
To evaluate the effects of freezing and thawing on Ca2+ transport and permeability, inside-out red cell membrane vesicles (IORCMV) are examined. Exposure to the cryoprotectant Me2SO as well as different cooling regimes on unprotected and cryoprotected vesicles do not affect the membrane Ca2+ transport. However, freezing and thawing increase the membrane permeability to sucrose.
A
A
Rubinacci
B
B
Fuller
F
F
Wuytack
W
W
De Loecker
D002118:Calcium / Q000097:blood*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005615:Freezing; D006801:Humans; D007700:Kinetics
D002118:Calcium; D004121:Dimethyl Sulfoxide