Automated 3'-H-phosphonate synthesis of oligodeoxyribonucleotides using amidine protected purine synthons.
Side reaction during phosphitylation of 5'-O-dimethoxytrityl-N2-isobutyryl-2'-deoxyguanosine was observed. As a suitable dG synthon, 5'-O-dimethoxytrityl-N2-dimethylaminomethyl-ene-3'-H-phosphonate was developed and used, along with 3'-H-phosphonates of 5'-O-dimethoxytrityl derivatives of dT, N4--benzoyl-dC and N6-dimethylaminoethylidene-dA, in automated synthesis of several oligodeoxyribonucleotides containing 30-40 bases.
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Praha.
Z
Z
Tocík
L
L
Arnold
J
J
Smrt
D000578:Amidines; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates; D011687:Purines
D000578:Amidines; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D063065:Organophosphonates; D011687:Purines