Fluorescence detection in automated DNA sequence analysis.
We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.
L M
LM
Smith
J Z
JZ
Sanders
R J
RJ
Kaiser
P
P
Hughes
C
C
Dodd
C R
CR
Connell
C
C
Heiner
S B
SB
Kent
L E
LE
Hood
D001331:Automation; D001483:Base Sequence; D003201:Computers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005456:Fluorescent Dyes; D008967:Molecular Biology / Q000295:instrumentation*; D009711:Nucleotides / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D009711:Nucleotides