Age dependent changes in the expression of Drosophila mitochondrial proteins.
A subset of 43 [35S]methionine labeled mitochondrial proteins, identified on two dimensional electrophoresis gels of whole body extracts of adult Drosophila, were compared between two age groups. These flies, which except for their spermatocytes consist entirely of postmitotic cells, were 6 days old and 38 days old, with the mean life span of this inbred strain being 32.5 days. Qualitative changes in any of these mitochondrial polypeptides were not found, but significant quantitative differences were observed. Quantitation of the 43 mitochondrial proteins was carried out by computer assisted microdensitometry of autoradiograms of the gel patterns. The total amount of isotope incorporated into the mitochondrial proteins of the senescent flies decreased to 71% of that of the young insects. This decrease was heterogeneously distributed among the 43 proteins; however, six remained unchanged and one protein (mol. wt = 75; pI = 7.4) was increased 2.4 times in the senescent flies. These data clearly provide evidence that the age-dependent changes in the expression of mitochondrial proteins are quantitative and not qualitative.
Fleming
J E
JE
Melnikoff
P S
PS
Latter
G I
GI
Chandra
D
D
Bensch
K G
KG
D000375:Aging; D000818:Animals; D004330:Drosophila / Q000254:growth & development*; D004591:Electrophoresis, Polyacrylamide Gel; D008715:Methionine / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D013462:Sulfur Radioisotopes
D011506:Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine