Development of a comparative dot ELISA for the detection of antibodies in blastomycosis.
We present a preliminary study utilizing an enzyme immunoassay for the detection of antibodies in blastomycosis using nitrocellulose membrane as the solid support. The comparative dot enzyme-linked immunosorbent assay (CDE) utilizing Blastomyces dermatitidis, Histoplasma capsulatum, and Coccidioides immitis antigens allows rapid screening of specimens and determination of a serum profile with respect to anti-Blastomyces antibodies. Sensitivity (76% or greater) and specificity (approximately 12% crossreactivity) determinations indicated that this visual assay may be an alternative antibody detection method for blastomycosis or other systemic fungal diseases.
Sato
H
H
Department of Biological Sciences, Idaho State University, Pocatello 83209.
Honigman
A S
AS
Scalarone
G M
GM
D000908:Antibodies, Fungal / Q000032:analysis*; D000946:Antigens, Fungal / Q000276:immunology; D001759:Blastomycosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G