N-Acylation of ethanolamine phospholipids by acyl transfer does not involve hydrolysis.
N-Acylethanolamine phospholipids occur in infarcted but not in normal canine myocardium. Their synthesis is catalyzed by a membrane-bound, Ca2+-requiring N-acyltransferase (transacylase) which transfers acyl groups from the sn-1 position of various phospholipids including phosphatidylethanolamine to the amino group of ethanolamine phospholipids. When dog heart mitochondria are incubated in media containing Ca2+ and H2(18)O, the resulting N-acylethanolamine phospholipids do not accumulate 18O in either the amide or 1-O-acyl groups. The results indicate that acyl transfer occurs without hydrolysis, most likely through an acyl-enzyme complex which may be covalently linked.
Schmid
P C
PC
Hormel Institute, University of Minnesota, Austin 55912.
Schmid
H H
HH
D000215:Acylation; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004285:Dogs; D004983:Ethanolamines / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D008929:Mitochondria, Heart / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism; D010103:Oxygen Isotopes; D010714:Phosphatidylethanolamines / Q000378:metabolism*
D004983:Ethanolamines; C022203:N-acylethanolamines; D010103:Oxygen Isotopes; D010714:Phosphatidylethanolamines; D002118:Calcium