High-performance liquid chromatographic analysis of oleandomycin in serum and urine.
The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C18 column employing acetonitrile-0.05 M phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phase-separation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 micrograms/ml (serum) and 1.0-25.0 micrograms/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of less than 5%.
Stubbs
C
C
Haigh
J M
JM
Kanfer
I
I
D002851:Chromatography, High Pressure Liquid; D004917:Erythromycin / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D009827:Oleandomycin / Q000032:analysis* / Q000097:blood / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D004917:Erythromycin; D009827:Oleandomycin