Modification of a laser-based flow cytometer for high-resolution DNA analysis of mammalian spermatozoa.
Modification of a Coulter EPICS V orthogonal laser-based flow cytometer/cell sorter allows resolution of X and Y mammalian sperm populations based on DNA content. The modification consists of beveling the sample injection tube situated in the flow chamber, adding a second fluorescence detector directly forward along the laser beam axis, and routing the collected fluorescence through an optical fiber bundle to one of the existing photomultiplier tubes. The X and Y chromosome-bearing spermatozoa from the bull, boar, and ram can be resolved using this system.
Johnson
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Pinkel
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D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D002469:Cell Separation; D004247:DNA / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D008297:Male; D012756:Sheep; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure; D013552:Swine
D004247:DNA