Comparative studies on partial microsequence-analysis of Bence-Jones proteins.
1. Sequence analyses of Bence-Jones proteins up to 15 amino acids from the N-terminus provide decision of subgroups. 2. Investigation of primary structure of 3 Bence-Jones proteins, monomer and dimer of a kappa type, is limited at position 9-10 using DABITC reagent, whereas DABITC/PITC double coupling method allows sequencing up to 20-21 amino acids. 3. Sequencing of tryptic peptides allows only determination of 6-9 amino acids. 4. Microsequencing of tryptic peptide T13a shows the allotypic variant, inv b+, of Bence-Jones proteins TRA and GAN.
Institute of Medical Biochemistry, University of Graz, Austria.
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D000595:Amino Acid Sequence; D001536:Bence Jones Protein / Q000652:urine; D009101:Multiple Myeloma / Q000652:urine; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D010446:Peptide Fragments; D001536:Bence Jones Protein; D014357:Trypsin