A model for tumorigenicity and metastatic potential: growth in 1.0% agar cultures.
We developed a method to determine the amount of work performed by cells through cell division in 1.0% agar cultures. There was no correlation between the cloning efficiencies of 1.0 and 0.3% agar cultures. Growth in 1.0% agar cultures correlated well with such malignant properties as tumorigenicity and the invasive and metastatic potentials. Our method revealed that metastatic MC and F cell lines possess different means of taking advantage of energy to proliferate against an environmental pressure from those possessed by nontumorigenic (ME and T-C3H) cell strain/line or nonmetastatic but tumorigenic (L,MR, and magc1) cell lines.
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
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T
Saiga
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Ohbayashi
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Tabuchi
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Midorikawa
D000362:Agar / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D002999:Clone Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D003470:Culture Media; D004548:Elasticity; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008954:Models, Biological; D009132:Muscles / Q000166:cytology; D009362:Neoplasm Metastasis; D009924:Organ Culture Techniques; D011312:Pressure; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology* / Q000502:physiology
D003470:Culture Media; D000362:Agar