Genomic characterization of the human DNA excision repair gene ERCC-1.
In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of +/- 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.
van Duin
M
M
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Koken
M H
MH
van den Tol
J
J
ten Dijke
P
P
Odijk
H
H
Westerveld
A
A
Bootsma
D
D
Hoeijmakers
J H
JH
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000302:isolation & purification; D004260:DNA Repair; D005796:Genes; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014162:Transfection; D014466:Ultraviolet Rays
D004247:DNA