On the mechanism of AgNO3-induced lipid peroxidation in erythrocytes.
In order to elucidate the possible mechanism of initiation of lipid peroxidation in AgNO3-treated erythrocytes, the effect of AgNO3 on hemoglobin-catalyzed peroxidation of phospholipid liposomes was studied. It was found that Ag+ significantly increases the rate of hemoglobin-catalyzed peroxidation. The inhibition of this effect by superoxide dismutase and catalase suggests that superoxide radical and H2O2 are involved. It was demonstrated also that Ag+ potentiates the hemoglobin autoxidation and increases the binding of hemoglobin to the erythrocyte membrane. On the basis of these findings a possible mechanism of initiation of peroxidation in AgNO3-treated erythrocytes is discussed.
S
S
Ribarov
L
L
Benov
I
I
Benchev
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008054:Lipid Peroxides / Q000097:blood*; D008081:Liposomes / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008706:Methemoglobin / Q000378:metabolism; D009994:Osmolar Concentration; D010108:Oxyhemoglobins / Q000378:metabolism; D012835:Silver Nitrate / Q000494:pharmacology*
D006454:Hemoglobins; D008054:Lipid Peroxides; D008081:Liposomes; D010108:Oxyhemoglobins; D008315:Malondialdehyde; D008706:Methemoglobin; D012835:Silver Nitrate