[Ultrastructure of capillary permeability in human brain tumors. 3: Mechanisms of contrast enhancement in non-glial tumors].
In order to elucidate mechanisms of contrast enhancement on computed tomography observed in non-glial tumors, tumors vessels were studied with conventional ultrathin section and freeze-fracture replica techniques. The materials were obtained from surgically removed specimens in 19 cases of tumors (6 of meningioma, 6 of hemangioblastoma, 5 of pituitary adenoma, and 2 of acoustic neurinoma). The following results were obtained. The common findings of these non-glial tumor vessels in ultrathin preparations were surface infoldings, increased pinocytotic vesticles and many fenestrations of endothelial cells, irregularity of basal laminae, and enlarged perivascular spaces. In freeze-fracture replicas of vascular endothelium, pinocytotic vesicles and fenestrations were 22 and 26 per micron2 on the average respectively. Tight junctions between endothelial cells were composed of one or two strands which appeared to be a discontinuous array of particles. As for the each non-glial tumor, menigiomas showed endothelial thickness and finger-like projections, variable lengths of tight junctions and marked enlargement of perivascular space which contained many collagen fibrils. Thinning of endothelium and many fenestrations were observed in hemangioblastomas, pituitary adenomas, and acoustic neurinomas. Fenestrations were most frequently observed in pituitary adenomas. The results indicate that extravasation of contrast material through fenestrations has an important role in marked contrast enhancement of non-glial tumors, in addition to the osmotic opening of tight junctions by contrast material. The irregular basal lamina and large perivascular space may also contribute to an increased extravasation of contrast material.
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Fukushima
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Shibata
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Inoue
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Mori
D000236:Adenoma / Q000648:ultrastructure; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000648:ultrastructure*; D002199:Capillary Permeability; D005260:Female; D005614:Freeze Fracturing; D006394:Hemangiosarcoma / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000648:ultrastructure; D008579:Meningioma / Q000648:ultrastructure; D008854:Microscopy, Electron; D008875:Middle Aged; D009464:Neuroma, Acoustic / Q000648:ultrastructure; D010911:Pituitary Neoplasms / Q000648:ultrastructure; D011856:Radiographic Image Enhancement; D014057:Tomography, X-Ray Computed