[Isolation of hexokinase II isoenzyme from the hyaloplasm of rat skeletal muscle].
A method for obtaining partially purified preparation of II isozyme of hexokinase from cytosol of the rat skeletal muscles has been suggested. According to the data of electrophoretic and kinetic analyses the preparation does not practically contain I isozyme of hexokinase and is characterized by high enzymatic activity. The obtained preparation of II isozyme of hexokinase may be successfully used for research of the adsorption mechanism controlling the enzyme activity.
E M
EM
Muntian
N Iu
NIu
Goncharova
D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D006593:Hexokinase / Q000302:isolation & purification*; D007527:Isoenzymes / Q000302:isolation & purification; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D051381:Rats
D007527:Isoenzymes; D006593:Hexokinase
Poluchenie II izozima geksokinazy iz gialoplazmy skeletnykh myshts krysy.