Structure and expression of the human creatine kinase B gene.
Various cDNAs for creatine kinase type B (CK-B) were isolated from human cDNA libraries using a 26-oligonucleotide guess-mer probe. One of the cDNAs appeared to be almost full-length and contained an open reading frame coding for the 381 amino acid residues of the human CK-B polypeptide. The nucleotide sequences of the translated region as well as the primary protein structure show a high degree of homology with known CK-B and CK-M sequences of other vertebrates. The level of CK-B RNA as a measure of CK-B gene activity was determined in various human tissues and cultured cells. Our results confirm that CK-B is expressed in a tissue-specific manner and give support to the previously proposed relation between CK-B gene activity and cell proliferation. Screening of genomic DNA with various cDNA regions as probes revealed that there is only one CK-B gene per haploid genome. Gene cloning and sequencing indicated that CK-B is coded for by a relatively small gene of 3.2 kb in size, which is partially overlapped by an HTF island (A. P. Bird (1986) Nature (London) 321, 557-558) with an extremely high G + C content at its 5' end.
Department of Human Genetics, University Nijmegen, Radboud Hospital, The Netherlands.
E C
EC
Mariman
C A
CA
Broers
C A
CA
Claesen
G I
GI
Tesser
B
B
Wieringa
D000595:Amino Acid Sequence; D001483:Base Sequence; D003402:Creatine Kinase / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D007527:Isoenzymes; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D003402:Creatine Kinase