A perfusion chamber for physiological studies with acutely dissociated neurons.
We describe a recording chamber that immobilizes acutely dissociated neurons on an ultra-fine mesh grid positioned above a moving stream of perfusate. This chamber is easily fabricated and has two attributes for single-electrode voltage-clamp or patch-clamp recording: (1) shallow immersion (less than 20 micron) of the neurons, and (2) stable recording with rapid perfusion rates.
Wonderlin
W F
WF
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
Weinreich
D
D
D004594:Electrophysiology / Q000295:instrumentation*; D009474:Neurons / Q000502:physiology*