In vitro hemopoiesis of marrow cells from myelodysplastic patients: effects of mitogen-stimulated spleen cell-conditioned medium and vitamin A and D derivatives.
We determined the ability of pokeweed mitogen-stimulated human spleen cells to support the growth and proliferation of hemopoietic progenitors from normal and myelodysplastic syndrome (MDS) patients, in the presence and absence of the maturation-inducing agents 13-cis-retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (vit D). The addition of various concentrations of vit D or RA to culture plates containing MDS patients' marrow cells stimulated myeloid colony growth. A higher number of myeloid colony-forming units-cell was noted in cultures containing vit D or RA in addition to pokeweed mitogen spleen-conditioned medium compared to these substances and placenta conditioned medium. When colony-forming units-cell from MDS patients was incubated with 2 X 10(-8) M vit D the percentage of monocyte-macrophage colonies was increased and the number of granulocyte colonies was decreased. Neither vit D nor RA affected the number of cells per colony. Our findings suggest that pokeweed mitogen spleen-conditioned medium provides a more potent source than placenta-conditioned medium for humoral factors that stimulate the growth of hemopoietic progenitors from MDS patients and that the use of pokeweed mitogen spleen-conditioned medium may improve studies of hemopoiesis in MDS patients.
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Fabian
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Riklis
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Tatarsky
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Nagler
D000362:Agar; D000368:Aged; D001853:Bone Marrow / Q000473:pathology / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D008297:Male; D009190:Myelodysplastic Syndromes / Q000473:pathology / Q000503:physiopathology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014807:Vitamin D / Q000494:pharmacology
D003470:Culture Media; D011043:Pokeweed Mitogens; D014807:Vitamin D; D014212:Tretinoin; D000362:Agar