Ethanol inhibition of granulocyte adherence.
Ethanol inhibits granulocyte adherence (GA) in whole blood. To determine the mechanism, GA was measured by the nylon fiber assay. In vitro addition of 300 mg/dl ethanol to whole blood caused a 28.1% decrease of GA compared with the control value (p less than 0.009) but did not affect GA of pure PMNs suspended in plasma or in HBSS. Similarly, GA measured in whole blood from intoxicated rabbits fell 48.8% from preintoxication values, but adherence of isolated postintoxication PMNs suspended in postintoxication plasma was not inhibited. Therefore, inhibition of GA by ethanol requires the presence of other cellular components of whole blood. To determine which cell(s) mediate the inhibition, PMNs were suspended with isolated blood components: platelet-rich and platelet-poor plasma, with and without the addition of mononuclear leukocytes and RBCs. Although the reconstitution of whole blood from its components allowed alcohol-induced inhibition of GA (decrease of 32.9%), none of the incomplete blood component mixtures demonstrated this effect. It was concluded that inhibition of GA by ethanol is mediated by an interaction between the components of whole blood.
C G
CG
Wlodaver
K
K
O'Neill
R R
RR
MacGregor
D000328:Adult; D000818:Animals; D001773:Blood Cells / Q000502:physiology; D001790:Blood Physiological Phenomena; D002448:Cell Adhesion / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009504:Neutrophils / Q000187:drug effects*; D010949:Plasma / Q000502:physiology; D011817:Rabbits; D012965:Sodium Chloride / Q000494:pharmacology
D000431:Ethanol; D012965:Sodium Chloride