Effect of lectin on nodulation by wild-type Bradyrhizobium japonicum and a nodulation-defective mutant.
The nodulation characteristics of wild-type Bradyrhizobium japonicum USDA 110 and mutant strain HS111 were examined. Mutant strain HS111 exhibits a delayed-nodulation phenotype, a result of its inability to initiate successful nodulation promptly following inoculation of the soybean root. Previously, we showed that the defect in initiation of infection leading to subsequent nodulation which is found in HS111 can be phenotypically reversed by pretreatment with soybean root exudate or soybean seed lectin. This effect is not seen after pretreatment with root exudates and lectins obtained from other plant species. Treatment of strain HS111 with as little as 10 soybean seed lectin molecules per bacterium (3.3 X 10 (-12) M) resulted in enhancement of nodule formation. Pretreatment of wild-type B. japonicum USDA 110 with soybean root exudate or seed lectin increased nodule numbers twofold on 6-week-old plants. Wild-type strain USDA 110 cells inoculated at 10(4) cells per seedling exhibited a delay in initiation of infection leading to subsequent nodulation. Wild-type cells pretreated in soybean root exudates or seed lectin did not exhibit a delay in nodulation at this cell concentration. Mutant strain HS111 pretreated in seed lectin for 0 or 1 h, followed by washing with the hapten D-galactose to remove the lectin, exhibited a delay in initiation of nodulation. Phenotypic reversal of the delayed-nodulation phenotype exhibited by strain HS111 was seen if incubation was continued for an additional 71 h in plant nutrient solution following 1 h of lectin pretreatment. Reversal of the delayed-nodulation phenotype of HS111 through lectin pretreatment was prevented by chloramphenicol or rifampin.(ABSTRACT TRUNCATED AT 250 WORDS)
L J
LJ
Halverson
G
G
Stacey
D002701:Chloramphenicol / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D007887:Fabaceae; D037102:Lectins / Q000494:pharmacology*; D000455:Medicago sativa; D019887:Peanut Agglutinin; D037121:Plant Lectins; D010946:Plants, Medicinal; D012230:Rhizobiaceae / Q000502:physiology*; D012293:Rifampin / Q000494:pharmacology; D030262:Soybean Proteins; D013025:Glycine max; D013559:Symbiosis; D010835:Phytohemagglutinins
D002701:Chloramphenicol; D003208:Concanavalin A; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D012293:Rifampin; D030262:Soybean Proteins; C017707:Ricinus communis agglutinin-1; C036554:dolichos biflorus agglutinin; C028824:soybean lectin; D010835:Phytohemagglutinins