An O2-based enzyme electrode for whole blood lactate measurement under continuous flow conditions.
An enzyme electrode-continuous flow system for whole blood lactate measurement has been produced using ultrafiltration membrane-entrapped lactate oxidase retained over an O2 sensor housed in a measuring chamber. Linearity was to 0.8 mmol/l. Oxalate inhibition of the enzyme necessitated the use of lithium heparin-fluoride as anticoagulant for blood samples. A continuous flow system was devised which eliminated differences in sample PO2; analysis of serum and plasma specimens showed good correlation with a routine fluorimetric method (y = 1.097x -0.494, r = 0.959 and y = 0.978x + 0.085, r = 0.952, respectively). The correlation for the enzyme electrode between whole blood and plasma lactate concentrations was y = 0.801x + 0.142, r = 0.986. The flow system should, furthermore, allow reagentless continuous in vivo extracorporeal lactate monitoring.
Weaver
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MR
Vadgama
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PM
D001322:Autoanalysis; D004566:Electrodes; D004800:Enzymes, Immobilized; D006801:Humans; D007773:Lactates / Q000097:blood*; D006899:Mixed Function Oxygenases; D010100:Oxygen / Q000032:analysis*
D004800:Enzymes, Immobilized; D007773:Lactates; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase; D010100:Oxygen