Synthesis and secretion of a nerve growth-stimulating factor by neonatal mouse astrocyte cells in vitro.
Neonatal mouse astroglial cells cultured in a serum-free medium synthesize and secrete a trophic growth factor which resembles nerve growth factor (NGF). The NGF-like factor reacts with antiserum to beta subunit of NGF (beta-NGF) and, after labeling with [35S] cystine, migrates similarly to purified mouse beta-NGF in SDS polyacrylamide gel electrophoresis and Sephadex G-100 gel filtration systems. The astrocyte cell NGF-like factor displays beta-NGF-like neurite growth-promoting activity for the clonal rat pheochromocytoma (PC-12) cell line and this bioactivity is blocked by beta-NGF antiserum. These results indicate that NGF-like factor synthesized and secreted by astroglial cells, is similar, if not identical, to beta-NGF from the mouse submandibular gland and further support a potential role for NGF in the central nervous system.
R H
RH
Tarris
M E
ME
Weichsel
D A
DA
Fisher
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D001681:Biological Assay; D002478:Cells, Cultured; D003470:Culture Media; D051379:Mice; D009414:Nerve Growth Factors / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D051381:Rats
D003470:Culture Media; D009414:Nerve Growth Factors