Binding studies of [3H]lyngbyatoxin A and [3H]debromoaplysiatoxin to the phorbol ester receptor in a mouse epidermal particulate fraction.
The preparation of [3H]lyngbyatoxin A by catalytic triton-proton exchange of lyngbyatoxin A and [3H]debromoaplysiatoxin by tritiation-debromination of aplysiatoxin is described. The dose-response curves for the binding of [3H]lyngbyatoxin A and [3H]debromoaplysiatoxin to a mouse epidermal particulate fraction are virtually the same as the one previously described for [3H]12-O-tetradecanoylphorbol-13-acetate ([3H]TPA). The specific binding of [3H]TPA, [3H]-lyngbyatoxin A and [3H]debromoaplysiatoxin to the mouse epidermal particulate fraction is inhibited to the same degree by unlabeled TPA, teleocidin, lyngbyatoxin A, aplysiatoxin and debromoaplysiatoxin. This study indicates that TPA, lyngbyatoxin A and debromoaplysiatoxin bind to the same high affinity receptor in mouse skin.
R E
RE
Moore
G M
GM
Patterson
M
M
Entzeroth
H
H
Morimoto
M
M
Suganuma
H
H
Hakii
H
H
Fujiki
T
T
Sugimura
D000818:Animals; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D004305:Dose-Response Relationship, Drug; D004817:Epidermis / Q000378:metabolism; D008235:Lyngbya Toxins / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D011493:Protein Kinase C; D011955:Receptors, Drug; D011971:Receptors, Immunologic / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D008235:Lyngbya Toxins; D011955:Receptors, Drug; D011971:Receptors, Immunologic; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; C017179:debromoaplysiatoxin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; C019457:lyngbyatoxin A