In vivo labeling of serotonin-containing neurons by 5,7-dihydroxytryptamine in Aplysia.
Intrahemocoelial administration of 5,7-dihydroxytryptamine (5,7-DHT) to Aplysia californica induces a transient (less than 4 h) behavioral alteration. About 5 weeks after 5,7-DHT treatment, 5-hydroxytryptamine (5-HT)-containing neurons develop dark brown pigmentation. These labeled 5-HT neurons have normal physiological and pharmacological properties when investigated electrophysiologically. This contrasts with the long-term neurotoxic effect of 5,7-DHT on vertebrate neurons. This technique will greatly facilitate visual identification of 5-HT-containing neurons and study of their physiology and actions.
Jahan-Parwar
B
B
New York State Department of Health, Wadsworth Center for Laboratories and Research, University at Albany, NY 12201.
Rozsa
K S
KS
Salanki
J
J
Evans
M L
ML
Carpenter
D O
DO
D015116:5,7-Dihydroxytryptamine; D000818:Animals; D001048:Aplysia; D001522:Behavior, Animal / Q000187:drug effects; D004102:Dihydroxytryptamines; D009474:Neurons / Q000032:analysis / Q000166:cytology* / Q000502:physiology; D012701:Serotonin / Q000032:analysis*
D004102:Dihydroxytryptamines; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin