The measurement of glycosylated albumin by reduction of alkaline nitro-blue tetrazolium.
A method is described for the measurement of glycosylated albumin in human plasma. Purification of albumin is achieved by a simple one-step chromatographic method, adapted for use as a batch analysis technique. The glycosylated albumin fraction is subsequently measured by a modification of the fructosamine assay (alkaline reduction of nitroblue tetrazolium). The method correlates with the plasma fructosamine assay (r = 0.85; p less than 0.001) and with an electrophoretic method for the measurement of HbA1 (r = 0.78; p less than 0.001).
Walker
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SW
Howie
A F
AF
Smith
A F
AF
D002845:Chromatography; D003920:Diabetes Mellitus / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D019270:Fructosamine; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced; D006595:Hexosamines / Q000097:blood; D006801:Humans; D009580:Nitroblue Tetrazolium; D012709:Serum Albumin / Q000032:analysis*; D013778:Tetrazolium Salts; D000093423:Glycated Serum Albumin
D019270:Fructosamine; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D006595:Hexosamines; D009580:Nitroblue Tetrazolium; D012709:Serum Albumin; D013778:Tetrazolium Salts; D000093423:Glycated Serum Albumin