Enzyme activities in the rabbit cornea during immunogenic keratitis.
In rabbits inflammation of the cornea was induced by intrastromal injection of horse serum. Between 2 and 4 weeks after injection, infiltration of the cornea with leukocytes and neovascularization could be observed. During this period, the rabbits were killed and their corneas analyzed for protein, alkaline phosphatase, acid phosphatase, N-acetyl-beta-D-glucosaminidase, and lactate dehydrogenase. The enzyme activities in the inflamed corneal stroma reflect the high lysosomal activity, which probably originates from the leukocytes. The enzyme activities in the epithelium indicate that the tissue is abnormal and undergoing repair processes.
N J
NJ
van Haeringen
A
A
Bolkova
J A
JA
Oosterhuis
J L
JL
van Delft
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D003315:Cornea / Q000201:enzymology*; D006736:Horses / Q000097:blood; D007154:Immune System Diseases / Q000201:enzymology*; D007114:Immunization; D007213:Indomethacin / Q000494:pharmacology; D007634:Keratitis / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011817:Rabbits
D007770:L-Lactate Dehydrogenase; D000135:Acid Phosphatase; D000118:Acetylglucosaminidase; D007213:Indomethacin