Control of gene expression by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Multiple dioxin-responsive domains 5'-ward of the cytochrome P1-450 gene.
In mouse hepatoma cells, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) stimulates cytochrome P1-450 gene transcription via the formation of TCDD-receptor complexes and the accumulation of the complexes in the nucleus. Here, we show that the DNA that flanks the 5'-end of the cytochrome P1-450 gene contains at least two discrete TCDD-responsive domains. Each domain has properties analogous to those of transcriptional enhancers. Each domain requires TCDD-receptor complexes for its function. There is no significant nucleotide sequence homology between the two TCDD-responsive domains.
P B
PB
Jones
L K
LK
Durrin
J M
JM
Fisher
J P
JP
Whitlock
D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004147:Dioxins / Q000494:pharmacology*; D005796:Genes / Q000187:drug effects*; D014644:Genetic Variation; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D010957:Plasmids; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D003577:Cytochrome P-450 Enzyme System