A procedure to increase the sensitivity of solid-phase immunoassays.
A strategy to increase the sensitivity of antibody detection is described. In this method, streptavidin is used as a cross-bridge between a biotin-conjugated second antibody and a DNA plasmid simultaneously labelled with biotin and 32P. With this simple approach antibodies can be detected with a significantly higher sensitivity as compared to the standard procedures using an 125I-labelled second antibody.
Blanco
J
J
Dixon
G H
GH
D000818:Animals; D000918:Antibody Specificity; D001345:Autoradiography; D001710:Biotin; D003101:Collodion; D004247:DNA; D006609:High Mobility Group Proteins / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D014337:Trout
D006609:High Mobility Group Proteins; D010761:Phosphorus Radioisotopes; D001710:Biotin; D003101:Collodion; D004247:DNA