Simultaneous analysis of low plasma levels of deuterium-labeled saturated and unsaturated fatty acids as t-butyldimethylsilyl esters.
A sensitive and accurate method for detection and quantitation of deuterated fatty acids in the presence of large amounts of unlabeled fatty acids is described using mass fragmentography in combination with the preparation of tertiarybutyldimethylsilyl esters (t-BDMS). The method has been applied to determination of deuterated stearic, oleic, elaidic and linoleic acids in human plasma lipoproteins following duodenal perfusion with a micellar mixture of acids. Over a concentration range of 10-1000 ng/ml, the average coefficient of variation for the linoleate was 3% and for the oleate (elaidate) ester was 2%.
Parsons
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Emken
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Marai
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Kuksis
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D002849:Chromatography, Gas / Q000379:methods; D003903:Deuterium; D005227:Fatty Acids / Q000097:blood*; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D017646:Organosilicon Compounds; D012825:Silicon
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D007202:Indicators and Reagents; D017646:Organosilicon Compounds; C009660:t-butyldimethylsilyl compounds; D003903:Deuterium; D012825:Silicon