The affinity of newly synthesized proteoglycan for hyaluronic acid can be enhanced by exposure to mild alkali.
The affinities for hyaluronic acid of newly synthesized proteoglycan from post-confluent rabbit chondrocyte cultures and purified bovine proteoglycan monomer were compared. In mixtures prepared at pH 6.8 the newly synthesized proteoglycan had the lower affinity; however, in mixtures incubated at pH 8.5 for 24 h before addition of hyaluronic acid, the newly synthesized proteoglycan exhibited a markedly higher affinity than the bovine monomer. The results suggest that proteoglycan secreted without associated link protein [Plaas, Sandy & Muir (1983) Biochem. J. 214, 855-864] has a low affinity for hyaluronate and that this may be increased during subsequent extracellular processing.
A H
AH
Plaas
J D
JD
Sandy
D000468:Alkalies / Q000494:pharmacology*; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D011485:Protein Binding / Q000187:drug effects; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits
D000468:Alkalies; D046911:Macromolecular Substances; D011509:Proteoglycans; D006820:Hyaluronic Acid