A dot-immunobinding assay for the serodiagnosis of Pasteurella multocida infection in laboratory rabbits.
A dot-immunobinding assay was developed to detect serum IgG specific for lipopolysaccharide of rabbit isolates of P. multocida. The assay detected serum IgG as early as 1 week after experimental subclinical nasal infection, whereas 8 weeks were required to detect antibody by a gel diffusion precipitin test. The assay was more reliable than nasal cultures, in that up to 46% of 16 weekly nasal washings of some infected rabbits failed to yield P. multocida. The bacterial antigen (proteinase k digested cell lysate) used in the assay reacted with IgG that did not cross-react with lipopolysaccharide antigens of B. bronchiseptica, P. pneumotropica or P. hemolytica. The assay is sensitive and specific, easily performed, cost effective, requires no special laboratory instruments and provides a permanent easily stored record.
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.
P J
PJ
Manning
G
G
Brackee
M A
MA
Naasz
D
D
DeLong
S L
SL
Leary
D000818:Animals; D000830:Animals, Laboratory; D003429:Cross Reactions; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology; D010326:Pasteurella Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D011817:Rabbits; D012698:Serologic Tests
D007074:Immunoglobulin G; D008070:Lipopolysaccharides