Marked effect of cations on the separation of spinach thylakoid chlorophyll-proteins on polyacrylamide gel electrophoresis at 0 degree C in the presence of alkanolammonium dodecyl sulfates.
The separation of spinach thylakoid chlorophyll-proteins on polyacrylamide gel electrophoresis at 0 degrees C in the presence of dodecyl sulfate is markedly influenced by the kind of surfactant cation in the media used for solubilization and electrophoresis. The mode of separation can thus be modulated through the cation selection. Three kinds of alkanolammonium dodecyl sulfates were tested and their abilities as to the dissociation of the molecular assemblies of chlorophyll-proteins were found to decrease in the following order: tris(hydroxymethyl)methylammonium, triethanolammonium and triisopropanolammonium. Comparison of the electrophoretic patterns obtained with different kinds of cation may help clarify the hierarchy of the molecular assemblies of chlorophyll-proteins.
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Kubo
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Takagi
D002412:Cations; D002734:Chlorophyll / Q000302:isolation & purification*; D002736:Chloroplasts / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants; D000644:Quaternary Ammonium Compounds; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013501:Surface-Active Agents
D002412:Cations; D010940:Plant Proteins; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D002734:Chlorophyll; D012967:Sodium Dodecyl Sulfate