Fluorometric ELISA for the detection and quantitation of metallothionein.
The development of a heterogeneous fluorometric ELISA for the detection and quantitation of metallothionein (MT) is described. A radioimmunoassay (RIA) previously developed in our laboratory is used as a reference assay to characterize the performance of the ELISA. The standard curves (logit-log regressions) that are typical of either assay have similar ranges (customarily from 20 000 to 100 pg of competing antigen); both assays are capable of quantitating MT in unknowns with 5-10% accuracy. Aspects of MT measurement in cytosols and physiological fluids are discussed.
Thomas
D G
DG
Linton
H J
HJ
Garvey
J S
JS
D000818:Animals; D003600:Cytosol / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005470:Fluorometry / Q000379:methods; D008297:Male; D008668:Metallothionein / Q000032:analysis* / Q000097:blood / Q000652:urine; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards
D008668:Metallothionein