Resolution of mouse hepatic cytochrome P-450 isozymes by chromatofocusing.
Constituent cytochrome P-450 isozymes from mouse hepatic microsomes were fractionated by chromatofocusing on Polybuffer Exchanger 94 over the pH range 5.3-8.3 and characterized by polyacrylamide gel electrophoresis. Eight isozymes were detected in fractions from unpretreated mice and seven from phenobarbitone-pretreated animals. Isozyme-containing fractions were reconstituted and shown to produce specific monohydroxy metabolites from both testosterone and delta 9-tetrahydrocannabinol.
Marriage
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D000818:Animals; D002845:Chromatography; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification* / Q000378:metabolism; D003579:Cytochrome Reductases / Q000302:isolation & purification; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D013739:Testosterone / Q000378:metabolism
D003902:Detergents; D007527:Isoenzymes; D013739:Testosterone; D003577:Cytochrome P-450 Enzyme System; D003579:Cytochrome Reductases