Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells.
Partially purified extracts from leaves of Melia azedarach L. (MA) exert a broad range of antiviral effects on DNA and RNA viruses. The effect of MA on different stages of Sindbis virus replicative cycle in BHK cells was investigated. Under one-step growth conditions MA afforded a greater than 90% inhibition in virus yield if added to the cell cultures 2 h before or after infection, and when added 4 h after infection MA still caused a greater than 80% inhibition. Analysis of early events following Sindbis virus infection showed that MA did not affect viral adsorption to or penetration in BHK cell. In contrast, viral RNA and protein synthesis was almost totally inhibited in cells pretreated with MA 2 h before infection, while cellular macromolecular synthesis was similar in MA-treated and untreated cell cultures.
Laboratorio de Virología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
M B
MB
Wachsman
E B
EB
Damonte
C E
CE
Coto
R A
RA
de Torres
D000998:Antiviral Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D010936:Plant Extracts / Q000494:pharmacology*; D012367:RNA, Viral / Q000096:biosynthesis / Q000187:drug effects; D012845:Sindbis Virus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D010936:Plant Extracts; D012367:RNA, Viral; D014764:Viral Proteins