Methyl mercury-induced combined inhibition of ATP regeneration and protein synthesis in reticulocyte lysate cell-free translation system.
Methyl mercury inhibits in vitro protein synthesis in the rabbit reticulocyte lysate cell-free translation system and simultaneously leads to reduction of the ATP/ADP index. It has been established that there is a close relationship (r = 0.86) between the rates of ATP resynthesis and protein synthesis in vitro within a wide range of the methyl mercury concentrations tested (0.0001-1.0 mumol/ml). Ammonia, CCl4, cycloheximide and pactamycin inhibit translation in vitro without affecting ATP resynthesis. Methanol does not cause substantial alterations of the parameters of the cell-free translation system. Thus, there are at least two essentially different causes of poison-induced in vitro translation blocking. Suppression of ATP resynthesis (methyl mercury) and direct effect on protein synthesis (cycloheximide, CCl4, etc.)
Kuznetsov
D A
DA
Zavijalov
N V
NV
Govorkov
A V
AV
Ivanov-Snaryad
A A
AA
D000244:Adenosine Diphosphate / Q000096:biosynthesis; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002474:Cell-Free System; D003513:Cycloheximide / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D008767:Methylmercury Compounds / Q000494:pharmacology*; D010142:Pactamycin / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*
D008767:Methylmercury Compounds; D010142:Pactamycin; D000244:Adenosine Diphosphate; D000641:Ammonia; D000255:Adenosine Triphosphate; D003513:Cycloheximide; D002251:Carbon Tetrachloride; D000432:Methanol