Use of two polysaccharide antigens in ELISA for the detection of antibodies to Echinococcus granulosus in sheep sera.
Polysaccharide antigens were obtained from either the secretions produced during in vitro cultivation of Echinococcus granulosus protoscoleces or from mouse hydatid cyst membranes by phenol extraction. When either of these antigens was used in an enzyme-linked immunosorbent assay antibody activities were detected in sera from sheep infected 27 or more weeks earlier with at least 100 E granulosus eggs. These antibody responses were significantly higher (P less than 0.05) than those of sheep infected with Taenia hydatigena or T ovis and tested with the E granulosus antigens. Very high cross-reacting antibody responses in sera from sheep recently infected with T hydatigena were only detected with the protoscoleces secretions antigen. Neither antigen was sufficiently sensitive or specific for serodiagnostic use. However, when sera were first tested with one antigen and then with the other, and only sera that were positive in both tests were regarded as positive, the overall sensitivity and specificity of this two antigen method increased to about 80 per cent.
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Wallaceville Animal Research Centre, Ministry of Agriculture and Fisheries, Upper Hutt, New Zealand.
Hamel
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KL
Mackle
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ZM
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000032:analysis*; D004443:Echinococcosis / Q000276:immunology / Q000662:veterinary*; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D011134:Polysaccharides / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology*; D013622:Taeniasis / Q000276:immunology / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011134:Polysaccharides