Modified extraction procedure for gas-liquid chromatography applied to the identification of anaerobic bacteria.
Chloroform and ether commonly are used as solvents to extract metabolic organic acids for analysis by gas-liquid chromatography in the identification of anaerobic bacteria. Because these solvents are potentially hazardous to personnel, modified extraction procedures involving the use of a safer solvent, methyl tert-butyl ether were developed which remained both simple to perform and effective for organism identification.
Thomann
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WR
Hill
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D000085:Acetates; D001421:Bacteria, Anaerobic / Q000145:classification* / Q000378:metabolism; D002264:Carboxylic Acids / Q000032:analysis*; D064227:Chlorofluorocarbons, Ethane; D005617:Chlorofluorocarbons, Methane; D002725:Chloroform; D002849:Chromatography, Gas; D004986:Ether; D004987:Ethers; D008738:Methyl Ethers; D008752:Methylene Chloride; D012449:Safety; D012997:Solvents
D000085:Acetates; D002264:Carboxylic Acids; D064227:Chlorofluorocarbons, Ethane; D005617:Chlorofluorocarbons, Methane; D004987:Ethers; D008738:Methyl Ethers; D012997:Solvents; C005850:1,1,1-trichloro-2,2,2-trifluoroethane; D004986:Ether; C043243:methyl tert-butyl ether; D008752:Methylene Chloride; C007650:ethyl acetate; D002725:Chloroform