Complete primary structure of the alpha 1-chain of human basement membrane (type IV) collagen.
We have determined the primary structure of the alpha 1(IV)-chain of human type IV collagen by nucleotide sequencing of overlapping cDNA clones that were isolated from a human placental cDNA library. The present data provide the sequence of 295 amino acids not previously determined. Altogether, the alpha 1(IV)-chain contains 1642 amino acids and has a molecular mass of 157625 Da. There are 1413 residues in the collagenous domain and 229 amino acids in the carboxy-terminal globular domain. The human alpha 1(IV)-chain contains a total of 21 interruptions in the collagenous Gly-X-Y repeat sequence. These interruptions vary in length between two and eleven residues. The alpha 1(IV)-chain contains four cysteine residues in the triple-helical domain, four cysteines in the 15-residue long noncollagenous sequence at the amino-terminus and 12 cysteines in the carboxy-terminal NC-domain.
Biocenter and Department of Biochemistry, University of Oulu, Finland.
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Soininen
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Haka-Risku
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Prockop
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Tryggvason
D000595:Amino Acid Sequence; D001483:Base Sequence; D001485:Basement Membrane / Q000032:analysis; D003094:Collagen / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000302:isolation & purification; D004220:Disulfides; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D012091:Repetitive Sequences, Nucleic Acid
D004274:DNA, Recombinant; D004220:Disulfides; D003094:Collagen; D004247:DNA