Cross-reaction of a monoclonal anti-filaggrin antibody with glycogen.
A mouse monoclonal anti human filaggrin antibody was found to bind keratohyaline granules of normal epidermis as well as of premalignant and malignant lesions in formalin-fixed tissue sections. In addition, an unexpected binding of this antibody with cells containing glycogen and other PAS positive substances was found, which could be abolished by adsorption of the anti-filaggrin antibody with glycogen or pretreatment of the sections with diastase.
Department of Dermatology, University of Erlangen-Nürnberg, Federal Republic of Germany.
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N
Vigneswaran
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E
Haneke
K P
KP
Peters
D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D000091344:Filaggrin Proteins; D006003:Glycogen / Q000276:immunology*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D006003:Glycogen