A step sensitive to pertussis toxin and phorbol ester in human neutrophils regulates chemotaxis and capping but not phagocytosis.
Treatment of human neutrophils with pertussis toxin (PT) abolishes chemotaxis in response to either platelet-activating factor (PAF) or f-Met-Leu-Phe (FMLP), and capping induced via the concanavalin A (Con A) receptor. These functional effects are accompanied by the inhibition of calcium mobilization by PAF, FMLP and Con A. The agent phorbol 12-myristate-13-acetate (PMA) also inhibits chemotaxis and capping as well as calcium mobilization by these receptors. In sharp contrast, neither PT, cholera toxin (CT), nor PMA, inhibits the phagocytosis of non-opsonized and opsonized Candida albicans, sheep erythrocytes or fluorescent latex beads. Our results suggest that receptor-initiated chemotaxis and capping involve a step that is sensitive to PT and PMA, and that phagocytosis is not regulated in a similar fashion.
Lad
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Olson
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Grewal
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D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003208:Concanavalin A / Q000031:analogs & derivatives; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D037342:Pertussis Toxin; D010587:Phagocytosis / Q000187:drug effects*; D010704:Phorbols / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D010704:Phorbols; D010972:Platelet Activating Factor; D010566:Virulence Factors, Bordetella; C042784:fluorescein isothiocyanate-concanavalin A; D003208:Concanavalin A; D009240:N-Formylmethionine Leucyl-Phenylalanine; D037342:Pertussis Toxin; D016650:Fluorescein-5-isothiocyanate; D013755:Tetradecanoylphorbol Acetate