Cytochemical demonstration of aspartate aminotransferase in the mossy-fibre system of the rat hippocampus.
A lead-salt procedure was used for the cytochemical demonstration of aspartate aminotransferase (AT) in the CA3 of the rat hippocampus. Cytoplasmic- and mitochondrial-AT isoenzymes were demonstrated in different neuronal structures, but not in astrocytes. Of special interest was the localization of cytoplasmic AT in most mossy-fibre boutons. This might indicate that cytoplasmic AT is responsible for the production of releasable glutamate in the glutamatergic/aspartatergic mossy fibre system.
Department of Anatomy, University of Würzburg, Federal Republic of Germany.
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P
Kugler
D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis*; D006624:Hippocampus / Q000032:analysis* / Q000648:ultrastructure; D006651:Histocytochemistry; D051381:Rats; D011919:Rats, Inbred Strains
D001219:Aspartate Aminotransferases