A comparison of the effect of two bovine serum albumin preparations on benzo(alpha)pyrene hydroxylase in rat liver and lung microsomes.
One of two commercial bovine serum albumin preparations caused decreases in rat liver and lung microsomal benzo(alpha)pyrene hydroxylase activities when measured by the fluorescence assay. The decreased activities were not due to a decreased recovery of a reaction product, 3-hydroxybenzo (alpha)pyrene, the presence of unmasked fatty acid binding sites or decreased content of cytochrome P450. The decreased enzyme activity may be due to a component present in the preparation. The results indicate that bovine serum albumin preparations should be carefully checked before use in the benzo(alpha)pyrene hydroxylase assay.
Rabovsky
J
J
White
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CC
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis*; D001579:Benzopyrene Hydroxylase / Q000032:analysis*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D008168:Lung / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013997:Time Factors
D012710:Serum Albumin, Bovine; D003577:Cytochrome P-450 Enzyme System; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases