[Estrogen- and progestin-receptor complexes and their interactions with rat liver cell nuclei in ontogenesis].
Steroid-receptor complexes (SRC) of estrogen and progestin were isolated from rat liver and purified 1500-2000-fold. The SRC within the composition of cytosol and purified 2000-fold were characterized by gel filtration of Sephadex G-100 and by DEAE-cellulose chromatography. The purified SRC from rat liver were bound to isolated liver cell nuclei of rats of various age (1.5, 6, 12 and 24 month-old). The maximal binding of progestin and estrogen SRC from rat liver was observed in homologous nuclei of 1.5-month-old animals. The binding of SRC by the nuclei decreased progressively with age, reaching its minimum in 24-month-old rats. The observed differences in the SRC binding by cell nuclei of experimental animals may be the cause of functional changes at various stages of ontogenesis.
E F
EF
Konoplia
G L
GL
Luksha
S K
SK
Savateev
A D
AD
Naumov
D000375:Aging; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism; D004054:Diethylstilbestrol / Q000378:metabolism; D005260:Female; D011372:Progestins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000302:isolation & purification / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000302:isolation & purification / Q000378:metabolism*
D011372:Progestins; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D004054:Diethylstilbestrol
Estrogen- i progestin-retseptornye kompleksy i ikh vzaimodeĭstvie s iadrami kletok pecheni krys v ontogeneze.