Terminating substrates of DNA polymerases: synthesis and functional study.
In order to study the mechanisms of DNA biosynthesis a number of modified nucleoside - substrates of DNA polymerases was synthesized. The absence of hydroxyl at 3'-position of ribose results in terminating properties of DNA biosynthesis of these analogues. A single step synthesis of triphosphates and alpha-thiotriphosphates of natural and 3'-modified 2'-deoxynucleosides is described.
Dyatkina
N B
NB
Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Arzumanov
A A
AA
D004247:DNA / Q000096:biosynthesis / Q000138:chemical synthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D007202:Indicators and Reagents; D013379:Substrate Specificity
D007202:Indicators and Reagents; D004247:DNA; D004259:DNA-Directed DNA Polymerase