[Production of glycerinated models of muscle fibers with phosphorylated myosin light chains].
A simple method for obtaining glycerinated muscle fibres of m. psoas of rabbit containing regulatory myosin light chains (LC2) of different levels of phosphorylation. The glycerination conditions stimulated endogenic kinase LC2 or phosphatase LC2. Glycerinated muscle fibres contained phosphorylated and dephosphorylated (levels of phosphorylation are 95 +/- 5%, and 5 +/- 5%, respectively) LC2 of myosin. To determine the level of phosphorylation the method of polyacrylamide gel electrophoresis in 8 M urea was modified.
Lebedeva
N N
NN
Wrotek
M
M
Shuvalova
L A
LA
Konkol
I
I
Borovikov
Iu S
IuS
D000818:Animals; D005990:Glycerol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008722:Methods; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D015879:Myosin Subfragments; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D010766:Phosphorylation; D011817:Rabbits
D015879:Myosin Subfragments; D010446:Peptide Fragments; D009219:Myosin-Light-Chain Kinase; D009218:Myosins; D005990:Glycerol
Poluchenie glitserinizirovannykh modeleĭ myshechnykh volokon s fosforilirovannymi legkimi tsepiami miozina.