Evaluation of a commercial kit for the routine detection of Clostridium difficile cytotoxin by tissue culture.
The Toxi-titer microtiter plate system (Bartels Immunodiagnostic Supplies, Inc., Bellevue, Wash.) is a simplified procedure for detecting the cytotoxin produced by Clostridium difficile in stool filtrates. In a parallel study of 74 stool specimens, results from the Toxin-titer system compared favorably with those from the conventional system. Our experience with the Toxin-titer system in testing 540 stool specimens was, in general, satisfactory, although a few problems with toxin control did occur. The method requires no specialized experience in tissue culture techniques and can therefore be adopted for routine use in clinical laboratories.
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D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000032:analysis*; D002460:Cell Line; D002470:Cell Survival; D003013:Clostridium; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000032:analysis* / Q000382:microbiology; D006801:Humans; D011933:Reagent Kits, Diagnostic
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D011933:Reagent Kits, Diagnostic; C031815:toxB protein, Clostridium difficile