Coeliac activity of the gliadin peptides CT-1 and CT-2.
The coeliac active peptide B 3142, which has been isolated from a peptic-tryptic digest of gliadin and which consists of 53 amino-acid sequences, was partially hydrolyzed with alpha-chymotrypsin. The two fragment peptides CT-1 (positions 1-22 of B 3142) and CT-2 (positions 23-53) were separated by high-performance liquid chromatography on octadecyl silica gel and purified by gel filtration on Biogel P2. The examination in the organ-culture test including 18 coeliac patients on normal diet and 7 control persons have shown that the toxicity is preserved after the chymotryptic treatment and that the peptides B 3142, CT-1 and CT-2 do not significantly differ from one another according to their coeliac-specific effect.
H
H
Wieser
H D
HD
Belitz
D
D
Idar
A
A
Ashkenazi
D000595:Amino Acid Sequence; D002446:Celiac Disease / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin; D005903:Gliadin / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D009924:Organ Culture Techniques; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000494:pharmacology; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D010940:Plant Proteins / Q000302:isolation & purification*
D010446:Peptide Fragments; D010455:Peptides; D010940:Plant Proteins; C048574:gliadin peptide CT-1; C048573:gliadin peptide CT-2; C044219:gliadin peptide B 3142; D005903:Gliadin; D002918:Chymotrypsin