Substrate specificity of DNA polymerases. II. 5-(1-Alkynyl)-dUTPs as substrates of the Klenow DNA polymerase enzyme.
A structure-activity relationship study was carried out with a sequence of 5-(1-alkynyl)-dUTPs in Klenow DNA polymerase enzyme-catalyzed reactions. Ability of modified dUTPs examined (up to the octynyl derivative) to serve as a substrate depended on both the length of substituent and the structure of DNA primer-template used.
Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.
L
L
Otvös
J
J
Szécsi
J
J
Sági
T
T
Kovács
D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000138:chemical synthesis*; D007700:Kinetics; D013379:Substrate Specificity
D003856:Deoxyuracil Nucleotides; C027078:deoxyuridine triphosphate; D004259:DNA-Directed DNA Polymerase