Determination of bucindolol and its major metabolites in plasma by capillary gas chromatography-mass spectrometry.
A method for determining bucindolol, a deuterated analogue and two metabolites in plasma samples is described. Analytes are obtained from plasma by liquid extraction of 1 ml of plasma at pH 10.5 with 7 ml of peroxide-free ether-hexane (4:1). Following derivatization with 50 microliters of N-methyl-N-trimethylsilyl trifluoroacetamide-pyridine (1:1) for 20 min at 65 degrees C the samples are analyzed by capillary column gas chromatography-mass spectrometry with selected-ion monitoring. Quantification is linear over the range 2-500 ng/ml. Single-day precision was typically 6.2% or better for bucindolol and 13% or better for the metabolites.
M J
MJ
Bartek
E H
EH
Kerns
R E
RE
Gammans
D G
DG
Gallo
D001711:Biotransformation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007700:Kinetics; D011412:Propanolamines / Q000097:blood* / Q000378:metabolism
D011412:Propanolamines; C024307:bucindolol