Isolation of leukocyte-free platelets from standard platelet concentrates by centrifugation.
We evaluated centrifugation techniques to isolate leukocyte-free platelets from standard platelet concentrates. Among various settings of centrifugation force and time, we found the setting at 300 X g for 10 minutes or 370 X g for 5 minutes to be efficient and reproducible for recovering sufficient quantities of platelets free of leukocytes.
Cott
M E
ME
Oh
J H
JH
Vroon
D H
DH
D002469:Cell Separation / Q000379:methods; D002498:Centrifugation / Q000379:methods; D006801:Humans; D007962:Leukocytes; D017713:Platelet Transfusion