A perfusion chamber for physiological studies with acutely dissociated neurons.
We describe a recording chamber that immobilizes acutely dissociated neurons on an ultra-fine mesh grid positioned above a moving stream of perfusate. This chamber is easily fabricated and has two attributes for single-electrode voltage-clamp or patch-clamp recording: (1) shallow immersion (less than 20 micron) of the neurons, and (2) stable recording with rapid perfusion rates.
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
W F
WF
Wonderlin
D
D
Weinreich
D004594:Electrophysiology / Q000295:instrumentation*; D009474:Neurons / Q000502:physiology*