Monoclonal antibodies to monkey brain choline acetyltransferase: production and immunohistochemistry.
Two monoclonal antibodies to monkey brain choline acetyltransferase (ChAT) were obtained. Immunoblot analysis indicated that both antibodies revealed two adjacent protein bands on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ChAT was demonstrated immunohistochemically by one of the antibodies in the motoneurons in the monkey brainstem and spinal cord. This antibody also revealed ChAT-positive terminal-like structures in the neuropils of lamina IX of the cervical spinal cord and interpeduncular nucleus.
Ichikawa
T
T
Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neurosciences, Japan.
Ishida
I
I
Hirata
Y
Y
Deguchi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D007700:Kinetics; D008253:Macaca mulatta; D013116:Spinal Cord / Q000166:cytology / Q000201:enzymology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002795:Choline O-Acetyltransferase