Cytostatic drug sensitivity test for human multiple myeloma, measuring monoclonal immunoglobulin produced by bone marrow cells in vitro.
An in vitro cytostatic drug sensitivity test for human multiple myeloma has been developed, predicting differences in sensitivity of the individual tumor to various anticancer drugs. Bone marrow preparations containing the tumor cells were incubated with cytostatic drugs and cultured for 10 days. Using an enzyme-linked immunosorbent assay we measured tumor products--monoclonal immunoglobulin and beta 2-microglobulin--in the culture supernatants. The reduction of these products in vitro due to the drugs administered was compared with the patients' further clinical course during treatment with different standard cytostatic drug regimens. We found a predictive value of more than 80% for this easily performed test.
Peest
D
D
Bartels
B
B
Dallmann
I
I
Schedel
I
I
Deicher
H
H
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis; D066298:In Vitro Techniques; D009101:Multiple Myeloma / Q000276:immunology*; D014410:Tumor Stem Cell Assay
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D007130:Immunoglobulin Idiotypes; D007136:Immunoglobulins