Liquid-chromatographic measurement of catecholamines and metabolites in plasma and urine.
In this general method for measuring the concentrations of neurotransmitters in body fluids by "high performance" liquid chromatography (HPLC), the procedures for extracting different biogenic amines from body fluids vary, but the basic chromatographic conditions are the same for all. We use citric/formic acid buffer as the mobile phase, a C18 column, and an electrochemical detector. Specificity is better than in previously reported methods. Because of its excellent cost efficiency, we consider this to be the method of choice for quantifying biogenic amines.
Department of Medicine, USC School of Medicine, Los Angeles 90033.
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Foti
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Kimura
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DeQuattro
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Lee
D000959:Antihypertensive Agents / Q000494:pharmacology; D002395:Catecholamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009647:Normetanephrine / Q000032:analysis*; D014642:Vanilmandelic Acid / Q000032:analysis*
D000959:Antihypertensive Agents; D002395:Catecholamines; D009647:Normetanephrine; D014642:Vanilmandelic Acid