Bovine brain factor promotes neurite extension from chick embryo dorsal root ganglia in vitro.
A protein with neurite-stimulating activity was isolated from bovine brain tissue lysosomal fraction. The organotypic culture of dorsal root ganglia (DRG) of 10-11-day-old chick embryo was used to test neurite-stimulating activity. A cation protein (16,000 Da) has been purified using a combination of extraction of acid-soluble proteins, preparative electrophoresis, and affinity chromatography on heparin-sepharose. This protein is active at 2-4 ng/ml inducing extensive neurite outgrowth in this bioassay compared to the control one. Many properties distinguish this factor from other known neuronotrophic factors.
Leningrad State University, I.P. Pavlov Institute of Physiology, Academy of Sciences, U.S.S.R.
V
V
Goncharova
A
A
Romanjuk
N
N
Chalisova
G
G
Akoev
E
E
Chumasov
O
O
Sotnikov
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002478:Cells, Cultured; D002642:Chick Embryo; D003712:Dendrites / Q000187:drug effects / Q000502:physiology*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D013347:Subcellular Fractions / Q000032:analysis
D009414:Nerve Growth Factors