In vitro survival of Echinococcus granulosus protoscolices in several media, at +4 degrees C and +37 degrees C.
As a first step towards hydatid disease control, the in vitro survival of protoscolices of Echinococcus granulosus was investigated for various media and temperatures. Higher percentage survival was obtained than previously reported: at 4 degrees C, 100% survival was obtained for 20 days in medium 199 (GIBCO) and for 25 days in hydatid fluid from the host of origin. Maximal survival was 30% at 55 days in these conditions. Flame cell activity was the criterion of choice for viability. At 37 degrees C survival rates were lower and morphological changes in protoscolices were observed.
Casado
N
N
Rodriguez-Caabeiro
F
F
Hernandez
S
S
D000818:Animals; D003470:Culture Media; D004444:Echinococcosis, Hepatic / Q000469:parasitology / Q000662:veterinary; D004445:Echinococcosis, Pulmonary / Q000469:parasitology / Q000662:veterinary; D004446:Echinococcus / Q000502:physiology*; D006734:Horse Diseases / Q000469:parasitology; D006736:Horses; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D013696:Temperature; D013997:Time Factors
D003470:Culture Media