Characterization of the alpha-MSH-like immunoreactivity in blood and cerebrospinal fluid of the rat.
We have investigated the nature of the alpha-melanocyte stimulating hormone-like immunoreactivity (alpha-MSH-LI) in blood and cerebrospinal fluid (CSF) of the rat. Blood and CSF from intact animals were subjected to high pressure liquid chromatography (HPLC) followed by a radioimmunoassay (RIA) specific for the C-terminal part of the alpha-MSH molecule. It appeared that in both body fluids the predominant alpha-MSH-LI co-migrated with synthetic alpha-MSH and not with its des-acetyl or di-acetyl analogues. We conclude that alpha-MSH is the predominant form of alpha-MSH-LI circulating in plasma and CSF of rats from our Wistar strain.
De Rotte
A A
AA
Verhoef
J
J
Andringa-Bakker
E A
EA
Van Wimersma Greidanus
T B
TB
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology; D010446:Peptide Fragments / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D010446:Peptide Fragments; D009074:Melanocyte-Stimulating Hormones