Bordetella bronchiseptica phase variation induced by crystal violet.
A method for effective induction of phase variation in Bordetella bronchiseptica by treatment with crystal violet (CV) is presented. When grown in CV-broth, phase I cells dissociated into three serial phases. Appearance of variant cells was observed simultaneously with the beginning of cell multiplication. The maximum effect of CV was obtained at a concentration of 8 micrograms/ml, when the proportion of variants in the population reached 100%. The main factors which affected phase variation were concentration of CV, culture age, and temperature of treatment. The phase variants obtained were phenotypically stable upon serial passages on Bordet-Gengou agar plates. By this treatment, no reversion of phase descendants to former phases was observed.
H
H
Ishikawa
Y
Y
Isayama
D000372:Agglutination Tests; D000818:Animals; D001884:Bordetella / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D003470:Culture Media; D014644:Genetic Variation; D005840:Gentian Violet / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D005840:Gentian Violet