[Comparison of chromosome aberrations, sister chromatid exchanges and unscheduled DNA synthesis in the evaluation of the mutagenicity of environmental factors].
Mutagenic character of formaldehyde in vivo was estimated by determining the level of chromosomal aberrations, sister chromatid exchanges and unscheduled DNA synthesis in human lymphocytes. It was found that in case of occupational exposure to formaldehyde the unscheduled DNA synthesis after thiophosphamide treatment in vitro was inhibited and spontaneous level of chromosomal aberrations increased. A negative correlation observed between the unscheduled DNA synthesis and sister chromatid exchanges indirectly confirmed a connection of these exchanges with the DNA repair. The comparison of the results obtained from evaluation of chromosomal aberrations, sister chromatid exchanges and unscheduled DNA synthesis permits suggesting that these methods estimate different sides of the mutagen interaction with a cell and should be considered as mutually complementary methods but not as interchangeable ones.
Chebotarev
A N
AN
Titenko
N V
NV
Selezneva
T G
TG
Fomenko
V N
VN
Katosova
L M
LM
D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D002869:Chromosome Aberrations / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D005557:Formaldehyde / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D009152:Mutagenicity Tests; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000395:Air Pollutants, Occupational; D009153:Mutagens; D005557:Formaldehyde
Sopostavlenie khromosomnykh aberratsiĭ, sestrinskikh khromatidnykh obmenov i vneplanovogo sinteza DNK pri otsenke mutagennosti vneshnesredovykh faktorov.