Purification of rat angiotensinogen.
A method of purifying rat angiotensinogen in three chromatography steps with a yield 3-4 times better than previous methods is described. Using chromatofocusing media for two steps and DEAE-affigel blue for the third step it was possible to separate angiotensinogen into three major peaks with pI of 5.25 (peak B), 4.80 (peak C) and 4.50 (peak D). Peaks B and C were completely purified with recoveries of 12% and 17% and specific activities of 21.8 and 20.0 micrograms AI/mg protein respectively. Analytical SDS-PAGE showed a 53,000 dalton band in both peaks with additional 51,000 and 57,000 dalton bands in peak C.
C
C
Sernia
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M
Davis
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Thomas
D000808:Angiotensinogen / Q000097:blood / Q000302:isolation & purification*; D000809:Angiotensins / Q000302:isolation & purification*; D000818:Animals; D002845:Chromatography / Q000379:methods; D008970:Molecular Weight; D051381:Rats
D000809:Angiotensins; D000808:Angiotensinogen