A reservoir model for repeated CSF access in the rabbit.
A simple, reliable and inexpensive subcutaneously implanted reservoir-catheter system method was developed for repeated sterile access to the rabbit brain ventricular system. The system provides an avenue for the repeated intrathecal administration of drugs and for the withdrawal of cerebrospinal fluid (CSF). Reservoirs were subcutaneously implanted under the scalp and connected to tubing inserted into the cisterna magna or the lateral ventricle. The systems successfully functioned in 38 of 41 animals (12 with cisternal magna catheters and 29 with lateral ventricle catheters), for up to 12 weeks following implantation. Patency of the systems were documented with X-rays and radiopaque injected contrast medium.
Department of Radiology, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
M A
MA
Samphilipo
S J
SJ
Hassenbusch
L B
LB
Grochow
F L
FL
Starr
J H
JH
Anderson
D000818:Animals; D002404:Catheterization; D002555:Cerebrospinal Fluid; D007276:Injections, Intraventricular; D011817:Rabbits; D013048:Specimen Handling / Q000295:instrumentation*