[Factors determining the frequency and pathways of the parthenogenetic development of ethanol-activated mouse oocytes].
Ethanol activates the eggs inside the mother upon intraperitoneal, rather than intragastric, injection. The eggs are also activated and engaged into parthenogenetic development when the eggs or the whole oviducts with the ovulated eggs are placed in a culture medium with ethanol. The intensity of the activating effect of ethanol in vitro and ways of parthenogenetic development depend both on the ethanol concentration and temperature. At a temperature below 17 degrees ethanol did not activate the mouse eggs. There is a temperature optimum for each ethanol concentration studied (from 2 to 6.6%), at and ways of parthenogenetic development depend both on the ethanol concentration and the ability of parthenogenetic embryos to develop until the blastocyst stage are determined by the efficiency of activation and depend on the selection of optimal conditions for the action of ethanol. Cytochalasin B or D did not enhance the activating action of ethanol on the mouse eggs. The mechanisms of ethanol action on the eggs are discussed.
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AP
Dyban
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EM
Noniashvili
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004624:Embryo Transfer; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D010312:Parthenogenesis / Q000187:drug effects*; D011003:Ploidies
D000431:Ethanol
Izuchenie faktorov, opredeliaiushchikh chastotu i puti partenogeneticheskogo razvitiia iaĭtsekletok myshi, aktivirovannykh étanolom.