Oxygen dependence of vitamin K-dependent carboxylase and vitamin K epoxidase.
A study of the oxygen requirements of the rat liver microsomal vitamin K-dependent carboxylase and vitamin K 2,3-epoxidase indicated that both enzymes had a Km for O2 in the range 60-80 microM. This value was not influenced by vitamin concentration, alterations in carboxylase substrate, Mn2+, or dithiothreitol, and is consistent with the hypothesis that both activities are catalyzed by the same enzyme.
J J
JJ
McTigue
J W
JW
Suttie
D000818:Animals; D019735:Carbon-Carbon Ligases; D004229:Dithiothreitol / Q000494:pharmacology; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D014813:Vitamin K Deficiency / Q000201:enzymology; D064417:Vitamin K Epoxide Reductases
D008345:Manganese; D006899:Mixed Function Oxygenases; D064417:Vitamin K Epoxide Reductases; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase; D004229:Dithiothreitol