Separation of cryptosporidium oocysts from fecal debris by density gradient centrifugation and glass bead columns.
A method was developed to obtain purified cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological studies.
M B
MB
Heyman
L K
LK
Shigekuni
A J
AJ
Ammann
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002417:Cattle; D002499:Centrifugation, Density Gradient; D003044:Coccidia / Q000302:isolation & purification*; D003458:Cryptosporidium / Q000276:immunology / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans
D000953:Antigens, Protozoan