Liquid-chromatographic determination of 4-hydroxyproline in urine.
In this method for 4-hydroxyproline in urine, hydroxyproline is derivatized with 4-chloro-7-nitrobenzofurazan, with subsequent estimation by reversed-phase "high-performance" liquid chromatography. The ranges for excretion of free and total hydroxyproline while the subjects were ingesting unrestricted diets were 2-29 and 122-374 mumol/24 h (n = 21), respectively, with no significant sex-related difference. A comparison with results by colorimetry indicated no significant differences: mean (n = 18) concentrations (mumol/L) of hydroxyproline in urine were 180 (SD 149) by the present method, 163 (SD 166) by colorimetry. For protein hydrolysate the respective values were 5.9 (SD 2.7) and 6.7 (SD 2.9).
Hughes
H
H
Hagen
L
L
Sutton
R A
RA
D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D008297:Male; D012016:Reference Values
D006909:Hydroxyproline