2,3-Diaminophenazine is the product from the horseradish peroxidase-catalyzed oxidation of o-phenylenediamine.
NMR and mass spectroscopic evidence has been obtained which indicates that the product of the oxidation of o-phenylenediamine by hydrogen peroxide, uncatalyzed or catalyzed by horseradish peroxidase, is 2,3-diaminophenazine. These results settle disparate literature descriptions. The process is most likely free radical in nature starting with the abstraction of a labile amino hydrogen atom.
Tarcha
P J
PJ
Abbott Laboratories, North Chicago, Illinois 60064.
Chu
V P
VP
Whittern
D
D
D002384:Catalysis; D006735:Horseradish Peroxidase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D010619:Phenazines / Q000032:analysis*; D010655:Phenylenediamines / Q000378:metabolism*
D010619:Phenazines; D010655:Phenylenediamines; C054312:2,3-diaminophenazine; C034193:1,2-diaminobenzene; D006735:Horseradish Peroxidase; D010544:Peroxidases