[Properties of muscle glycerol-3-phosphate dehydrogenase in rabbits with Brown-Pearce carcinoma].
Specific activity of glycerol-3-phosphate dehydrogenase, estimated in direct and reverse reactions, was decreased, as compared with normal state, without any alterations in the ratio of activities, in muscle homogenates of rabbits with Brown-Pearce carcinoma. The enzymatic activity was decreased by an order of magnitude in homogenates of the tumor as compared with muscle homogenates of the tumor-bearing animals simultaneously with relative stimulation of the reverse reaction. Specific activity of the crystalline enzyme from muscles of tumor-bearing animals was decreased as compared with normal tissues but the ratio of activities was 2-fold increased due to the reverse reaction. In the carcinoma activity of one of the three enzyme fractions, obtained after isoelectrofocusing, was similar to the control values; in the second fraction the ratio of activities was decreased due to inhibition of the direct reaction, the third fraction was inactive. Amino acid composition of crystalline glycerol-3-phosphate dehydrogenase, isolated from tumor-bearing animals, was different in content of 5 amino acids from the enzyme amino acid composition of controls, thus suggesting the structural differences of the proteins from these fractions.
L T
LT
Litvinenko
L L
LL
Khomiakova
V A
VA
Gaevskaia
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002284:Carcinoma, Brown-Pearce / Q000201:enzymology*; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis* / Q000378:metabolism; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D013379:Substrate Specificity
D000596:Amino Acids; D007527:Isoenzymes; D005993:Glycerolphosphate Dehydrogenase; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+)
Svoĭstva myshechnoĭ glitserol-3-fosfatdegidrogenazy krolikov s kartsinomoĭ Brouna-Pirs.