Comparison of chrysene metabolism in epithelial human bronchial and Syrian hamster lung cells.
Chrysene is metabolized to 1-, 2-, 3-, and 4-hydroxychrysene and trans-1,2- as well as trans-3,4-dihydroxydihydrochrysene in human and Syrian hamster epithelial lung cells as indicated by GC/MS analysis, whereas K-region oxidation is at most a very minor pathway. Cells of a permanent clonal line of fetal hamster lung metabolized 97% of the chrysene whereas fetal human bronchial epithelial cells converted 24% of the substrate within 8 days incubation. In human cells oxidation at the 3,4-position predominates, whereas oxidation at the 1,2-position is the major pathway in hamster cells. Indication for a bay-region oxidation of chrysene in hamster cells has been obtained.
Biochemisches Institut für Umweltcarcinogene, Ahrensburg-Grosshansdorf, F.R.G.
J
J
Jacob
G
G
Grimmer
G
G
Raab
M
M
Emura
M
M
Riebe
U
U
Mohr
D000818:Animals; D001980:Bronchi / Q000378:metabolism*; D002478:Cells, Cultured; D002911:Chrysenes / Q000378:metabolism*; D006224:Cricetinae; D004848:Epithelium / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008168:Lung / Q000378:metabolism*; D010084:Oxidation-Reduction; D010616:Phenanthrenes / Q000378:metabolism*; D013045:Species Specificity
D002911:Chrysenes; D010616:Phenanthrenes; C031180:chrysene