Analysis of fluorescence decay kinetics measured in the frequency domain using distributions of decay times.
We describe the theoretical and practical aspects of analyzing complex fluorescence decay kinetics using continuous distributions of decay times. Our analysis uses frequency-domain data, provides for global analysis of multiple data sets and includes the possibility of excited-state processes. Simulated data were used to estimate the types of distributions which can be reasonably recovered from the measurements. Additionally, we describe a variety of distributions recovered from experimental data. For mixtures of one, two or three exponentially decaying fluorophores we recovered narrow lifetime distributions, which are essentially identical to a multiexponential decay. Similarly, a two-state excited-state reaction also yielded a narrow distribution with negative preexponential factors. The presence of time-dependent spectral relaxation of labeled lipids results in a wide distribution of decay times, which becomes narrower for faster relaxation rates at higher temperatures. Hence, the decay-time distributions appear to be sensitive to the dynamics of the environment surrounding the fluorophore. Additionally, distributions of decay times were observed to result from transient effects in collisional quenching, from energy transfer in the presence of a range of donor-to-acceptor distances, and for several single-tryptophan proteins.
University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201.
J R
JR
Lakowicz
H
H
Cherek
I
I
Gryczynski
N
N
Joshi
M L
ML
Johnson
D004735:Energy Transfer; D007700:Kinetics; D011506:Proteins; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors; D014364:Tryptophan
D011506:Proteins; D014364:Tryptophan