Preparation of a bovine monoclonal antibody to testosterone by interspecies fusion.
This communication reports the first successful attempt to produce a hybridoma cell line secreting bovine immunoglobulin to a small hapten, starting with peripheral blood lymphocytes, rather than spleen or lymph node cells. A heteromyeloma line, sensitive to selective media, was made by fusing NS1/1-Ag4-1 mouse myeloma cells with bovine peripheral blood lymphocytes. This cell line was then fused with blood lymphocytes from a steer immunised with a testosterone immunogen. Cell cultures were screened using an ELISA specific for bovine antibodies to testosterone. Following repeated cloning, a cell line was established which secretes moderate levels of a specific, high affinity antibody to testosterone. This particular cell line has significant potential for veterinary application and the successful fusion demonstrates the possibilities of heteromyelomas for the development of non-murine monoclonal antibodies.
Groves
D J
DJ
Department of Biochemistry, University of Surrey, Guildford.
Morris
B A
BA
Clayton
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002417:Cattle / Q000276:immunology*; D002460:Cell Line; D006822:Hybrid Cells / Q000276:immunology*; D008297:Male; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D013739:Testosterone / Q000276:immunology*
D000911:Antibodies, Monoclonal; D013739:Testosterone