Primary structure of limulus anticoagulant anti-lipopolysaccharide factor.
A potent anticoagulant, anti-lipopolysaccharide (LPS) factor, found in limulus hemocytes inhibits the LPS-mediated activation of limulus coagulation cascade and shows an antibacterial action against R-types of Gram-negative bacteria (Morita, T., Ohtsubo, S., Nakamura, T., Tanaka, S., Iwanaga, S., Ohashi, K., and Niwa, M. (1985) J. Biochem. (Tokyo) 97, 1611-1620). The complete amino acid sequence of this substance was determined by sequencing the peptides obtained by selective proteolytic cleavage. The NH2-terminal end of anti-LPS factor was pyroglutamic acid. Anti-LPS factor had two variant residues at position 36 and the COOH-terminal end, respectively. The following sequence was assigned to anti-LPS factor, and it was also confirmed by fast atom bombardment mass spectrometry. less than EGGIWTQLALALVKNLATLWQSGDFQFLGHE (formula; see text) Limulus anti-LPS factor consisted of a single chain of 102 residues with 2 half-cystines in disulfide linkage. Its NH2-terminal region up to 20 residues was highly hydrophobic, and positively charged residues were clustered mainly within the disulfide loop. By searching the homologous sequence in known protein sequences with that of anti-LPS factor, we found a structural homology between anti-LPS factor and alpha-lactalbumin/lysozyme family.
J
J
Aketagawa
T
T
Miyata
S
S
Ohtsubo
T
T
Nakamura
T
T
Morita
H
H
Hayashida
T
T
Miyata
S
S
Iwanaga
T
T
Takao
Y
Y
Shimonishi
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000925:Anticoagulants / Q000032:analysis*; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D007447:Invertebrate Hormones / Q000032:analysis*; D007768:Lactalbumin / Q000032:analysis; D013058:Mass Spectrometry; D009113:Muramidase / Q000032:analysis; D011487:Protein Conformation
D000596:Amino Acids; D000925:Anticoagulants; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D007447:Invertebrate Hormones; C034705:antilipopolysaccharide factor (Limulus); D007768:Lactalbumin; D009113:Muramidase