Possible mechanism for differences in sensitivity to cis-platinum in human prostate tumor cell lines.
Two human prostate tumor cell lines which exhibit a 2.4-fold difference in sensitivity to cis-platinum (cis-Pt) were found to possess slightly different sulphydryl contents, and the presence of a metallothionein-like zinc-binding protein was demonstrated in the line exhibiting relative resistance to cis-Pt. Although these factors have been postulated to play a role in the mechanism(s) of resistance to cis-Pt in other cell types, preliminary data in this report suggest that differences found in drug uptake and subsequent binding to DNA are most likely responsible for variations in cis-Pt sensitivity displayed by these prostate tumor cell lines.
Metcalfe
S A
SA
Cain
K
K
Hill
B T
BT
D002460:Cell Line; D002850:Chromatography, Gel; D002945:Cisplatin / Q000378:metabolism / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000378:metabolism; D004351:Drug Resistance; D006801:Humans; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D004273:DNA, Neoplasm; D013438:Sulfhydryl Compounds; D008668:Metallothionein; D002945:Cisplatin