Catecholamine content of the preovulatory follicles of the domestic hen.
The role of catecholamines in ovarian function of the domestic hen has not been examined extensively. The aim of this study was first to determine the location of catecholamines in the preovulatory follicle of the domestic hen. Second, norepinephrine (NE), epinephrine (EPI) and dopamine (DA) were measured in the isolated theca layer of the five largest preovulatory follicles at specific times during the ovulatory cycle and changes in catecholamine content were correlated with ovarian events. The five largest preovulatory follicles were removed from chickens at 24, 18, 12, 6 and 2 h before ovulation of the largest (F1) follicle. Theca and granulosa layers were isolated, frozen, weighed and prepared for measurements of catecholamines by the double isotope radio-enzymatic assay. Catecholamines were localized primarily in the theca layer with only small amounts present in the granulosa layer. Norepinephrine was present in the theca layer in concentrations 6- and 30-fold those of EPI and DA, respectively. The content of NE and EPI in the theca layer of the F1 follicle was significantly (p less than 0.01) higher at 6 h before ovulation than at other times for the F1 follicle. In contrast, NE and EPI content of the theca layer of second (F2) and third (F3) largest follicles did not change during the ovulatory cycle. The content of DA was elevated (p less than 0.05) at 12 h before ovulation in F1 and F2 follicles. There was a significant reduction in NE in the theca layer of the fifth largest (F5) follicle between 24 and 18 h before ovulation of the F1 follicle.(ABSTRACT TRUNCATED AT 250 WORDS)
J M
JM
Bahr
L K
LK
Ritzhaupt
S
S
McCullough
L A
LA
Arbogast
N
N
Ben-Jonathan
D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002645:Chickens / Q000502:physiology*; D004298:Dopamine / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism*; D010060:Ovulation; D013799:Theca Cells / Q000378:metabolism
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine