A simple procedure for the purification of neutral salt soluble type I collagen from skin.
Intact monomeric type I collagen was purified from fetal bovine skin by a simple and time saving procedure. Saline precipitates of mixed skin collagens, in 4 M NaCl, were extracted with a limited volume of dilute acetic acid, taken in the proportion of 1 ml per g of original wet skin; NaCl in the precipitate was not removed by dialysis. The salt concentration in the extraction medium in the above conditions, selectively solubilized type I collagen.
M L
ML
Speranza
G
G
Valentini
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003094:Collagen / Q000302:isolation & purification*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus; D012867:Skin / Q000032:analysis*; D012965:Sodium Chloride / Q000494:pharmacology*; D012995:Solubility
D000085:Acetates; D000596:Amino Acids; D012965:Sodium Chloride; D003094:Collagen; D019342:Acetic Acid