Pantethine lipomodulation: evidence for cysteamine mediation in vitro and in vivo.
Recent human studies suggest rapid in vivo hydrolysis of the lipid-lowering drug, pantethine, to the vitamin pantothenic acid and the small aminothiol compound, cysteamine. To test whether the active agent is a hydrolysis product, we repeated three experimental models of pantethine's effect with pantothenate and cysteamine. In vitro experiments with human fetal fibroblasts showed equivalent modulation of cholesterol and methyl sterol synthesis by pantethine, cysteamine, or cystamine (the disulfide of cysteamine), but pantothenate had no effect. Similarly, in vivo experiments with 0.5% cholesterol-fed rabbits showed oral pantethine or equimolar cystamine significantly lowered plasma cholesterol, while pantothenate, cystine, and 2-hydroxyethyl disulfide did not. Lastly, diabetic male rats (40 mg/kg streptozotocin) fed 0.1% pantethine and lower plasma free fatty acids after 2 weeks than controls, an effect not seen with pantothenate and largely duplicated by cystamine. The efficacy of pantethine has previously been attributed to altered vitamin metabolism and increased coenzyme A concentration. Pantethine did increase CoA levels 45% in rat liver homogenates while equivalent amounts of cystamine or pantothenate did not. However, a causal relationship between CoA levels and pantethine's action as a hypolipemic agent has never been shown. At least in 3 independent experimental models, the lipomodulating effect of pantethine appears instead to be mediated by the hydrolysis product cysteamine.
Wittwer
C T
CT
Department of Pathology, University of Utah Medical School, Salt Lake City 84132.
Graves
C P
CP
Peterson
M A
MA
Jorgensen
E
E
Wilson
D E
DE
Thoene
J G
JG
Wyse
B W
BW
Windham
C T
CT
Hansen
R G
RG
D000818:Animals; D002478:Cells, Cultured; D003065:Coenzyme A / Q000378:metabolism; D003543:Cysteamine / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D006937:Hypercholesterolemia / Q000188:drug therapy / Q000378:metabolism; D050356:Lipid Metabolism; D008297:Male; D010204:Pantetheine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D013438:Sulfhydryl Compounds; D010204:Pantetheine; D003543:Cysteamine; C005425:pantethine; D003065:Coenzyme A