[Effectiveness of the cryoprotective effect of glycerol and polyethylene glycol on plasma membranes].
The barrier properties of erythrocyte membranes were studied under freezing-thawing in cryopreservatives based on glycerol or polyethylene glycol Mm 1500 Da (PEG-1500) (at cryoprotectant concentrations from 0.5 to 30%). The state of the membrane barrier function in reconstituted and native erythrocytes subjected to rapid (200-400 degrees C per min) freezing has been registered by spectrophotometry, flame photometry, and radiolabel analysis as judged by labeled compound release (hemoglobin, K+, [14C]sucrose, 86Rb+). It is demonstrated that the effective cryoprotection of erythrocyte membranes in reconstituted erythrocytes is attained at cryoprotectant concentrations in the medium of 0.5-1% or in erythrocytes of 8-30%.
V V
VV
Riazantsev
A K
AK
Gulevskiĭ
D001793:Blood Preservation; D003451:Cryoprotective Agents; D004910:Erythrocyte Membrane / Q000187:drug effects*; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011188:Potassium / Q000097:blood
D003451:Cryoprotective Agents; D011092:Polyethylene Glycols; D005990:Glycerol; D011188:Potassium
Effektivnost' krioprotektornogo deĭstviia glitserina i poliétilenglikolia na plazmaticheskie membrany.