Fractionation of plant and animal high mobility group chromosomal proteins by ion-exchange and reversed-phase high-performance liquid chromatography.
A method for purifying wheat high mobility group (HMG) chromosomal proteins using a combination of weak cation-exchange and reversed-phase high-performance liquid chromatography (HPLC) is described. Previously reported HPLC systems devised for fractionating HMG proteins of vertebrate animals are not effective for the plant proteins. The system described here can be used for the fractionation of both plant and animal HMG proteins.
Department of Genetics, North Carolina State University, Raleigh 27695-7614.
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Spiker
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Bates
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LJ
Arwood
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis; D006609:High Mobility Group Proteins / Q000032:analysis*; D010944:Plants / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D014908:Triticum / Q000032:analysis
D006609:High Mobility Group Proteins; D012967:Sodium Dodecyl Sulfate