Specific carbodiimide-binding mechanism for the selective modification of the aspartic acid-101 residue of lysozyme in the carbodiimide-amine reaction.
A mechanism for the selective modification of Asp-101 in hen egg-white lysozyme with an amine nucleophile catalyzed by 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide hydrochloride (EDC) was investigated using ethanolamine as a nucleophile at pH 5.0 and room temperature. In the presence of N-acetyl-D-glucosamine (NAG) and its oligomers [(NAG)n, n = 2 and 3] under the conditions with which about 90% of lysozyme was calculated to form complexes, the formation of Asp-101 modified lysozyme decreased markedly but to different degrees, that is (NAG)3 was the most and NAG the least effective. When the lysozyme derivative, in which Trp-62 in the active site cleft was oxidized to oxindolealanine (Ox-62 lysozyme), was used in place of native lysozyme, the formation of Asp-101 modified derivative decreased to about half, which was similar to the decrease in the presence of (NAG)2. In the presence of 0.5 M NaCl, on the other hand, the formation of Asp-101 modified lysozyme was considerably enhanced. From these observations, it is concluded that EDC binds to the active site cleft of lysozyme to specifically activate Asp-101. The affinity of EDC to the active site of lysozyme is partly due to the hydrophobic interaction of EDC with the Trp-62 residue at sub-site B of lysozyme. EDC is an activating reagent for carboxyl groups unlike most active site-directed reagents which produce final products directly. Therefore, the active site-directed nature of EDC was very useful because it made it possible to selectively introduce various amines as needed at a particular carboxyl group of lysozyme.
Kuroki
R
R
Yamada
H
H
Imoto
T
T
D000117:Acetylglucosamine / Q000378:metabolism; D000588:Amines / Q000378:metabolism*; D001224:Aspartic Acid; D001665:Binding Sites; D001667:Binding, Competitive; D002234:Carbodiimides / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019856:Ethanolamine; D004983:Ethanolamines; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000378:metabolism*; D008433:Mathematics; D009113:Muramidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D011108:Polymers; D011485:Protein Binding; D012965:Sodium Chloride; D014364:Tryptophan
D000588:Amines; D002234:Carbodiimides; D004983:Ethanolamines; D011108:Polymers; D001224:Aspartic Acid; D012965:Sodium Chloride; D019856:Ethanolamine; D014364:Tryptophan; D009113:Muramidase; D005022:Ethyldimethylaminopropyl Carbodiimide; D000117:Acetylglucosamine