Liquid chromatography used in diagnosis of a rare hemoglobin combination: hemoglobin S/LeporeBoston.
"High-performance" liquid chromatography (HPLC), applied to hemoglobin analysis, is decidely more sensitive and gives better resolution than do routine electrophoretic methods. Here we present a case with a rare double heterozygote hemoglobin S/LeporeBoston, originally diagnosed as homozygous hemoglobin S by routine electrophoretic methods. Using a gradient elution weak cation-exchange HPLC technique, we could separate hemoglobin S and hemoglobin LeporeBoston and make the correct diagnosis. This case demonstrates how HPLC can be a useful adjunct to routine electrophoresis.
R A
RA
Wessels
B B
BB
Rogers
C N
CN
Ou
R
R
Alcorn
G J
GJ
Buffone
D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood / Q000235:genetics; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D008297:Male
D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; C031751:hemoglobin Lepore Boston