High-performance liquid chromatography of adult human bronchoalveolar lavage: assay for phospholipid lung profile.
High-performance liquid chromatography has been used to separate pulmonary phospholipids from adult human bronchoalveolar lavage. A solvent system consisting of acetonitrile-water (80:20) as solvent A and pure acetonitrile as solvent B was used with a silica column (Bio-Sil HP 10) coupled to an Si-100 Polyol precolumn. A linear gradient from 87.5 to 25% of solvent B was found to separate all biologically relevant surfactant phospholipids in the following sequence and composition: phosphatidic acid (1.1%), phosphatidylglycerol (10.6%), phosphatidylinositol (9.9%), phosphatidylethanolamine (3.6%), phosphatidylserine (4.5%), phosphatidylcholine (60.8%), sphingomyelin (8.1%) and lysophosphatidylcholine (1.6%). These results were very similar to the phospholipid pattern obtained by two-dimensional thin-layer chromatography. It is concluded that high-performance liquid chromatography is a useful and rapid method for the separation of phospholipids in biological fluids containing pulmonary surfactant.
U
U
Pison
E
E
Gono
T
T
Joka
U
U
Obertacke
M
M
Obladen
D000328:Adult; D001980:Bronchi / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D008168:Lung / Q000032:analysis*; D008297:Male; D010743:Phospholipids / Q000032:analysis*; D011650:Pulmonary Alveoli / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D007507:Therapeutic Irrigation
D010743:Phospholipids