Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient.
Cryptosporidium oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by density gradient centrifugation. This two-step method yielded highly concentrated oocysts largely free of bacteria and fecal debris.
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E
Waldman
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S
Tzipori
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JR
Forsyth
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D003044:Coccidia / Q000302:isolation & purification*; D003457:Cryptosporidiosis / Q000469:parasitology; D003458:Cryptosporidium / Q000166:cytology / Q000302:isolation & purification* / Q000472:pathogenicity; D005243:Feces / Q000469:parasitology*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology