[Rapid microdetermination of 2 aminoglycosides by high-performance liquid chromatography in the plasma, bile and urine. Correlation of high-performance liquid chromatography and fluorescence polarization for plasma assays].
An HPLC method for determination of dibekacin and amikacin in various biological samples is described. A step gradient elution and fluorescence detection carried out through an on line post column derivatization system with OPT is used. The chromatographic conditions allow simple and rapid preparation, so that a result can be obtained within twenty minutes. The small volume required (150 microliters) and the rapid sample clean-up are advantageous for routine use even in pediatric patients. The good sensitivity (dibekacin: 0,44 mumol/l - amikacin: 0,35 mumol/l), reproducibility and specificity indicate it to be suitable for pharmacokinetic studies. Results obtained from HPLC were in excellent agreement with those of FPIA for both dibekacin and amikacin (r greater than or equal to 0,98).
R
R
Garraffo
P
P
Lapalus
P
P
Dellamonica
D000583:Amikacin / Q000032:analysis* / Q000097:blood / Q000652:urine; D001646:Bile / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003982:Dibekacin / Q000032:analysis* / Q000097:blood / Q000652:urine; D005454:Fluorescence Polarization; D006801:Humans; D007612:Kanamycin / Q000031:analogs & derivatives*; D008832:Microchemistry
D003982:Dibekacin; D007612:Kanamycin; D000583:Amikacin
Microdétermination rapide de deux aminosides par chromatographie liquide haute performance dans le plasma, la bile et l'urine. Corrélation chromatographie liquide haute performance-polarisation de fluorescence pour les dosages plasmatiques.