The sequence of the M RNA of an isolate of La Crosse virus.
The middle-size (M) genomic RNA of a New York State, U.S.A. isolate of La Crosse (LAC) virus has been cloned by a random priming procedure and its nucleotide sequence determined by the dideoxy method. The RNA was found to be 4526 nucleotides in length and to have a base composition of 34.2% U, 27.8% A, 20.6% C and 17.4% G. There is a single, long open reading frame in the viral complementary RNA that contains sufficient information to code for a protein of 1441 amino acids. In these respects, as in many others, the LAC virus M RNA and its encoded protein were very similar, if not identical, to those previously reported by other investigators for the closely related snowshoe hare virus. The M RNAs of the two viruses show 79% nucleotide sequence homology and 89% homology in the amino acid sequence of their encoded proteins. Several algorithms for predicting surface residues, as well as the Chou-Fasman rules for predicting secondary structure, were used to compare the LAC virus and snowshoe hare virus M gene proteins. These analyses identified 39 sites on the proteins as those most likely to be linear antigenic determinants that might contribute to the differences between the two viruses.
Grady
L J
LJ
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
Sanders
M L
ML
Campbell
W P
WP
D000595:Amino Acid Sequence; D000954:Antigens, Surface / Q000235:genetics; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D002043:Bunyaviridae / Q000235:genetics*; D002141:Encephalitis Virus, California / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000954:Antigens, Surface; D000956:Antigens, Viral; D012367:RNA, Viral; D014764:Viral Proteins