Primary structure of the glycans of porcine pancreatic lipase.
The glycan primary structure of the main glycopeptide fraction obtained by pronase and carboxypeptidase A digestions of porcine pancreatic lipase has been investigated by 500-MHz 1H-NMR spectroscopy and methylation analysis. The results demonstrate that the glycopeptide fraction was a mixture containing the following structures: (formula; see text)
Fournet
B
B
Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq., France.
Leroy
Y
Y
Montreuil
J
J
Dĕcaro
J
J
Rovery
M
M
van Kuik
J A
JA
Vliegenthart
J F
JF
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000302:isolation & purification; D008049:Lipase / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D013552:Swine
D006023:Glycoproteins; D008049:Lipase