Influence of glucose on collagen and protein production in cultured human skin fibroblasts from diabetic and non-diabetic subjects.
The effect of glucose on extracellular production of collagen and protein was studied in cultured human skin fibroblasts. Fibroblasts from 8 non-diabetics, 8 type 1 and 12 type 2 diabetic subjects were cultured. Incorporation of tritium labeled proline into medium collagen and protein was studied at glucose concentration 1-7 mg/ml (5.6-38.9 mM). A 75% increase in collagen production was noted by cells from type 1 diabetics at glucose concentration of 5 mg/ml, as compared to 1 mg/ml. A considerably lower although significant increase was also noted at this level by cells from non-diabetics. Glucose had no effect on collagen and minimal on protein production by cells from type 2 diabetic subjects. The results indicate an effect of glucose on extracellular matrix formation in diabetes mellitus which might be relevant for diabetic angiopathy.
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Kjellström
D000328:Adult; D000368:Aged; D002452:Cell Count / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003920:Diabetes Mellitus / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D012016:Reference Values; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D014316:Tritium
D014316:Tritium; D003094:Collagen; D011392:Proline; D005947:Glucose