The DNase I generated disomal series is coherent to 16N. Implications for coiling models of chromatin structure.
The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end-labelling and field-inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16N level and as such is incompatible with the most structurally acceptable coiling models. We propose that this is evidence for the general unsuitability of coiling models and is support for the existence of simple 'back-to-back' double-stranded structures within chromatin.
School of Biology, Flinders University, Bedford Park, South Australia.
S J
SJ
Davis
L A
LA
Burgoyne
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000097:blood / Q000648:ultrastructure; D003850:Deoxyribonuclease I; D004912:Erythrocytes / Q000648:ultrastructure; D009690:Nucleic Acid Conformation
D002843:Chromatin; D004247:DNA; D003850:Deoxyribonuclease I