The potential use of implanted radiolabelled bovine nasal cartilage in dialysis tubing to evaluate agents affecting cartilage degradation in vivo.
Cartilage which undergoes extensive autolysis in vitro (spontaneous or stimulated) is characterized by proteoglycan loss. Experimental conditions and inhibitor profils studies suggest neutral metalloproteinases induce the autolysis. In these preliminary studies we compared the degradation of Na2 35SO4 labeled bovine nasal cartilage (BNC) plugs placed in dialysis tubing in vitro and in vivo. The dialysis tubing was used to exclude large molecules (molecular weights greater than 2000) like proteinases, and proteinase inhibitors (e.g. alpha 2-macroglobulin) but not potential test agents from the implanted cartilage. Cartilage autolysis occurred with live tissue but not with heat-killed tissue in both the in vitro and in vivo systems. In addition retinoic acid and phenanthroline were effective when placed inside or outside the dialysis tubing. A potentially useful procedure to evaluate agents which affect cartilage degradation is described.
Stuart Pharmaceuticals Division of ICI Americas Inc., Wilmington, Delaware 19897.
G
G
DiPasquale
J
J
Conaty
D
D
Dea
K
K
Perry
D000818:Animals; D001329:Autolysis / Q000378:metabolism*; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D003956:Dialysis / Q000295:instrumentation; D066298:In Vitro Techniques; D010618:Phenanthrolines / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D010618:Phenanthrolines; D014212:Tretinoin