Monoclonal antibodies against genetically manipulated hepatitis B core antigen.
Four different hybridoma clones secreting anti-HBcAg antibodies were constructed by fusing cells of the mouse myeloma line SP2/0 with lymphocytes from mice immunized with bacterially produced HBcAg. The monoclonal antibodies were immunologically characterized and used for HBcAg detection by ELISA. This monoclonal-antibody-based assay was compared with ELISA based on polyclonal human anti-HBcAg IgG for sensitivity and specificity. The monoclonal antibody reacted specifically both with the bacterially produced HBcAg and HBcAg isolated from human liver, but did not react with HBeAg. The human polyclonal antibody reacted with HBcAg, but also with HBeAg.
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
I
I
Hlozánek
E
E
Korec
V
V
Dostálová
J
J
Stará
J
J
König
V V
VV
Bichko
A
A
Seichertová
E J
EJ
Gren
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D006512:Hepatitis B Core Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008099:Liver / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011994:Recombinant Proteins / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D006512:Hepatitis B Core Antigens; D011994:Recombinant Proteins