Excretion profiles of the mycotoxin deoxynivalenol, following oral and intravenous administration to sheep.
The excretion profiles of deoxynivalenol (DON) and metabolites (DON glucuronide conjugate, 3 alpha, 7 alpha,15-trihydroxytrichothec-9,12-diene-8-one (DOM-1), and DOM-1 glucuronide conjugate) were defined in male sheep following either intravenous (iv) or oral administration of the toxin at levels of 0.5 and 5.0 mg DON/kg body wt, respectively. After iv dosing, urinary DON levels declined in a biphasic fashion with an average elimination half-life (terminal phase) of 1.2 hr, diminishing to baseline concentrations by 8 hr. Maximum urinary excretion rates for the two major metabolites identified (conjugated DON, conjugated DOM-1) occurred 0.5-1.5 hr after dosing, exhibiting elimination half-lives of 2.2 and 3.1 hr, respectively. Total recovery accounted for only about 66.5% of the dose: 63.0% in the urine (24.1% DON, 21.2% conjugated DON, 0.5% DOM-1, 17.2% conjugated DOM-1) and 3.5% in bile (made up almost completely of conjugated DOM-1). The peak biliary excretion rate for conjugated DOM-1 was found to occur within 1 hr postdosing, which rapidly declined to baseline levels by 5 hr. Following oral administration, urinary excretion rates of the major metabolites (DON, conjugated DON, conjugated DOM-1) reached maximum 6-9 hr post-treatment, and declined exponentially with t 1/2 values of 3.2, 4.0, and 5.0 hr, respectively. Urinary and biliary recovery of administered DON averaged approximately 7.1%: 7.0% in urine (2.1% DON, 3.6% conjugated DON, 0.06% DOM-1, 1.2% conjugated DOM-1) and 0.11% in bile (predominantly conjugated DOM-1).(ABSTRACT TRUNCATED AT 250 WORDS)
Prelusky
D B
DB
Veira
D M
DM
Trenholm
H L
HL
Hartin
K E
KE
D000284:Administration, Oral; D000818:Animals; D001646:Bile / Q000378:metabolism; D001711:Biotransformation; D005243:Feces / Q000032:analysis; D006207:Half-Life; D007275:Injections, Intravenous; D008297:Male; D012717:Sesquiterpenes / Q000652:urine*; D012756:Sheep; D013997:Time Factors; D014255:Trichothecenes / Q000008:administration & dosage / Q000378:metabolism / Q000652:urine*
D012717:Sesquiterpenes; D014255:Trichothecenes; C048238:deepoxy-deoxynivalenol; C007262:deoxynivalenol