Detection of factors that interact with the human beta-interferon regulatory region in vivo by DNAase I footprinting.
We have used a DNAase I genomic footprinting procedure to detect interactions between cellular factors and the regulatory sequences of the human beta-interferon gene. Prior to induction with poly(I)-poly(C), factors that bind to DNA are detected in one region located between -94 and -167 from the mRNA cap site, and in another region located between -68 and -38. After induction these factors dissociate and another factor binds to a region located between -77 and -64. Correlation of these footprints with the effects of deletions in the regulatory region of the beta-interferon gene (accompanying paper) suggest that the factors that bind prior to induction are repressor molecules, while the component that binds after induction is a transcription factor. Dissociation of the repressor molecules from the DNA after induction may allow the transcription factor to bind to and activate the beta-interferon promoter. Thus, the beta-interferon gene may be controlled by a negative regulatory mechanism.
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D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D004247:DNA / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000378:metabolism; D003720:Densitometry; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D008297:Male; D008957:Models, Genetic; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D011070:Poly I-C / Q000494:pharmacology; D013268:Stimulation, Chemical
D004268:DNA-Binding Proteins; D007370:Interferon Type I; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003850:Deoxyribonuclease I; D011070:Poly I-C