[The role of A-cells in affecting the immunostimulating effect of F(ab')2-fragments].
The immunoregulatory effect of F(ab')2 fragments on normal rabbit IgG and that preincubated with A-cells from spleen have been compared. Both products were tested for their ability to enhance primary immune response of rabbit spleen cells to SRBC. It was demonstrated that low molecular mass product appeared after F(ab')2 fragments incubation with A-cells at 37 degrees C and possessed immunostimulating activity similar to that of initial F(ab')2 fragments. In addition, it was shown that F(ab')2 reduction to monovalent Fab' fragment with the following alkylation of SH-group abolished the ability of Fab' fragment to enhance the immune response. It may signify that half cystein Fab' fragment residue is essential for processing of the fragment in A-cells and (or) for immune response enhancement.
Bartova
L M
LM
Margulis
G U
GU
Kulagina
N N
NN
Kul'berg
A Ia
AIa
D000478:Alkylation; D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D002448:Cell Adhesion; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D006868:Hydrolysis; D007140:Immunoglobulin Fab Fragments / Q000032:analysis / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D011817:Rabbits; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013438:Sulfhydryl Compounds / Q000032:analysis
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D013438:Sulfhydryl Compounds
Rol' A-kletok v realizatsii immunostimuliruiushchego deĭstviia F(ab')2-fragmentov.