Nuclear transplantation in mouse embryos: assessment of recipient cell stage.
Enucleated zygotes were compared with enucleated two-cell embryos as recipients for donor nuclei from eight-cell embryos. Only one or two cleavage divisions were observed when eight-cell nuclei were transplanted to enucleated zygotes. Development of enucleated two-cell embryos containing a transplanted eight-cell nucleus was appreciably better with 51% (45/89) of the embryos forming blastocysts in vitro and 42% (25/60) initiating implantation. Of these, eleven implantation sites on Day 10 of gestation were examined histologically and two contained normally developing embryos. No development was observed beyond Day 12 of gestation. These observations indicate that a major transition occurs between the zygote and two-cell stage that results in the two-cell recipient being more compatible with the eight-cell nucleus than with the zygote.
Robl
J M
JM
Gilligan
B
B
Critser
E S
ES
First
N L
NL
D000818:Animals; D004622:Embryo, Mammalian / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D053652:Nuclear Transfer Techniques; D009924:Organ Culture Techniques; D015053:Zygote / Q000502:physiology*