Formation of 11 beta-hydroxysteroids requires the integrity of the microfilament network in adrenocortical cells.
In order to determine the role of microfilaments in adrenal steroidogenesis, we have studied the effect of cytochalasin B, a microfilament-disrupting agent, on the kinetics of [3H] pregnenolone conversion to labelled metabolites by frog interrenal tissue in vitro. Cytochalasin B (5 x 10(-5)M) induced a 50 to 70% decrease in corticosterone, 18-hydroxycorticosterone and aldosterone biosynthesis while the formation of progesterone and 11-desoxycorticosterone was not affected. These results suggest that microfilaments interfere in the conversion of 11-desoxycorticosterone to corticosterone probably by controlling the movement of 11-desoxycorticosterone from the reticulum to the mitochondria.
Groupe de Recherche en Endocrinologie Moléculaire, UA CNRS 650, Unité Alliée à l'INSERM, Université de Rouen, Mont-Saint-Aignan, France.
M
M
Feuilloley
P
P
Netchitaîlo
C
C
Delarue
I
I
Lihrmann
H
H
Vaudry
D008841:Actin Cytoskeleton / Q000502:physiology*; D000302:Adrenal Cortex / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003345:Corticosterone / Q000096:biosynthesis; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D006914:Hydroxysteroids / Q000096:biosynthesis*; D066298:In Vitro Techniques; D011284:Pregnenolone / Q000378:metabolism; D011898:Ranidae
D006914:Hydroxysteroids; D003571:Cytochalasin B; D011284:Pregnenolone; D003345:Corticosterone