An in vitro and in vivo study on mutagenic activity of fluoranthene: comparison between cytogenetic studies and HPLC analysis.
The polycyclic aromatic hydrocarbon, fluoranthene (FT), has been shown to induce SCEs in vitro in CHO cells in the presence of metabolic activation. Negative results were obtained in vivo in mice. The HPLC analysis performed to investigate the presence of metabolites of FT both in the serum of treated mice and in culture medium of CHO cells confirmed some differences between in vitro and in vivo metabolism.
F
F
Palitti
R
R
Cozzi
M
M
Fiore
F
F
Palombo
C
C
Polcaro
G
G
Perez
E
E
Possagno
D000818:Animals; D001711:Biotransformation; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005449:Fluorenes / Q000633:toxicity*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D010053:Ovary; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects
D005449:Fluorenes; D009153:Mutagens; C007738:fluoranthene; D001973:Bromodeoxyuridine; D003078:Colchicine