Rapid method for measuring plasma low-density lipoprotein turnover using high-performance gel exclusion chromatography.
High-performance gel exclusion chromatography using flow-through radioactivity monitoring was employed for rapid measurement of low-density lipoprotein (LDL) turnover. Iodinated LDL was injected into five fasting rabbits. Serial blood samples were obtained and small aliquots (0.1-0.2 ml) were injected into the chromatographic system using 0.2 M Tris-acetate buffer (pH 7.0). Radioactivity in lipoproteins was measured by an on-line gamma counter (Flo-One) attached to the high-performance liquid chromatograph and in a regular gamma counter after collecting the fractions. Sequential ultracentrifugation was also used to separate lipoproteins, and the radioactivity in each fraction was measured. The Flo-One method was faster; however, the efficiency of Flo-One varied with the amount of radioactivity and therefore it was necessary to inject the same amount of radioactivity to get comparable values.
M C
MC
Williams
C G
CG
Stenoien
R S
RS
Kushwaha
D000818:Animals; D001055:Apolipoproteins B / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000097:blood*; D011817:Rabbits; D014461:Ultracentrifugation
D001055:Apolipoproteins B; D008077:Lipoproteins, LDL