Identification of a mouse serum protein increased by administration of an antitumor polysaccharide, PSK, as a variant of mouse transferrin and some of its biological activities.
One of the so-called LC components, the content of which is increased in the serum of PSK (antitumor polysaccharide) treated mice, was purified by repeated ion-exchange column chromatography on DEAE-Sephadex A-50. The purified protein, designated as LC-2, was identified as a variant of mouse transferrin, which is a serum beta 1-globulin having an iron-binding capacity. The absorption spectrum and ultraviolet circular dichroism (CD) curve of LC-2 were identical with those of mouse transferrin. The molecular weight, isoelectric point and amino acid composition of LC-2 were in good agreement with the results obtained previously for mouse transferrin. However, the carbohydrate content of LC-2 was different from that of mouse transferrin. This protein restored the depressed spleen cell response of tumor-bearing mice to concanavalin A (Con A) and promoted the metabolism of proteose-peptone induced peritoneal macrophages obtained from tumor-bearing mice. Furthermore, it had weak but definite antitumor activity against Sarcoma 180 cells in vivo.
Kojima
E
E
Mitsuno
T
T
Osawa
T
T
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002849:Chromatography, Gas; D002942:Circular Dichroism; D003208:Concanavalin A / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005947:Glucose / Q000378:metabolism; D006466:Hemopexin / Q000494:pharmacology; D006868:Hydrolysis; D007526:Isoelectric Point; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D011509:Proteoglycans / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000097:blood / Q000276:immunology; D012513:Sarcoma, Experimental / Q000097:blood / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet; D014168:Transferrin / Q000302:isolation & purification / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D011509:Proteoglycans; D014168:Transferrin; D003208:Concanavalin A; C010770:polysaccharide-K; D006466:Hemopexin; D005947:Glucose