High affinity binding of the large T protein of polyoma virus to a genomic mouse DNA sequence.
We purified a fragment of mouse DNA to which the large T protein of polyoma virus was bound in chromatin prepared from transformed mouse cells. This sequence, which is not repeated to a measurable extent within the mouse genome, does not show any significant homology to the viral ori region, except in a short region, which comprises a sequence related to the consensus for recognition by large T proteins ((A,T)GPuGGC). This region of pCG4 was confirmed by in vitro binding assays to be essential for T antigen binding.
Unité 273 de l'INSERM, Université de Nice, France.
C
C
Galup
P
P
Léopold
L
L
Trejo-Avila
P
P
el Baze
M
M
Rassoulzadegan
P
P
Gaudray
F
F
Cuzin
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000378:metabolism*; D001483:Base Sequence; D002472:Cell Transformation, Viral; D002843:Chromatin / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid
D000957:Antigens, Viral, Tumor; D002843:Chromatin; D004268:DNA-Binding Proteins; D009698:Nucleoproteins; D004247:DNA