Evaluation of growth of avian mycoplasmas on bile salt agar and in bile broth.
All nine Mycoplasma iowae strains and one strain of M gallinarum grew on 0.05 per cent 'bile' agar medium. The colony size of M iowae on this agar medium was similar to the size obtained on bile-free mycoplasma agar. One strain each of M maculosum, M arginini and M bovis also grew on 0.05 per cent bile agar. However, one strain each of M gallisepticum and M meleagridis were inhibited at this concentration. Six of the nine strains of M iowae were also resistant to 1 per cent 'bile' in broth medium but all were resistant to 0.5 per cent. The resistance of M iowae to 0.5 per cent 'bile' in broth may be a useful characteristic for differentiating it from some of the other avian mycoplasmas.
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Canada.
M
M
Shah-Majid
S
S
Rosendal
D000362:Agar; D001647:Bile Acids and Salts; D009174:Mycoplasma / Q000254:growth & development*
D001647:Bile Acids and Salts; D000362:Agar