Quantitative surface studies of protein adsorption by infrared spectroscopy. II. Quantification of adsorbed and bulk proteins.
Attenuated total reflectance Fourier transform infrared spectra of surface-adsorbed proteins are correlated with concentration measurements determined by 125I-labeled proteins. This paper demonstrates that linear correlations between the intensity of the major bands of proteins and the quantity of proteins can be obtained for human albumin and immunoglobulin G up to surface concentrations of approximately 0.25 microgram/cm2. A poorer correlation was observed for human fibrinogen. A linear correlation was also observed between the concentration in the bulk solution and the major bands of albumin up to a concentration of 60 mg/ml.
Fink
D J
DJ
Bio-Integration, Inc., Columbus, Ohio 43212.
Hutson
T B
TB
Chittur
K K
KK
Gendreau
R M
RM
D000327:Adsorption; D006801:Humans; D007457:Iodine Radioisotopes; D007784:Lactoperoxidase; D011506:Proteins / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013499:Surface Properties
D007457:Iodine Radioisotopes; D011506:Proteins; D007784:Lactoperoxidase