New methodology for the synthesis of 2'(3')-O-aminoacyl oligoribonucleotides related to the 3'-terminus of aa-tRNA.
A new synthetic approach to the title compounds is reported. It is based on the phosphotriester methodology and uses a unique combination of protecting groups (Fmoc and Ph for the aglycons; DMT, CPTr and Mthp for the carbohydrate hydroxy functions; 2-CIPh for the phosphodiester bonds; BPOC for amino acid). This enables selective deprotection of the blocked oligonucleotide intermediates in two steps (oximate and acid treatments) to yield the 2'(3')-O-aminoacyl oligoribonucleotides suitable for biochemical investigations.
Department of Chemistry, Michigan Cancer Foundation, Detroit 48201.
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MD
Hagen
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Scalfi-Happ
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Happ
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Chládek
D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D012346:RNA, Transfer, Amino Acyl / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012346:RNA, Transfer, Amino Acyl