Effects of pre- and post-thaw cell-to-cell contact and trypsin on survival of freeze-thaw damaged mammalian cells.
When multicellular spheroids, which simulate small bits of tissue, are exposed to a freeze-thaw (FT) cycle, the survival of the individual cells in the spheroid is higher if the cells of the spheroid are trypsinized and plated as single cells immediately after thawing than if the spheroid is allowed to remain intact for 4 hr and then trypsinized for plating. The results imply either that cell-to-cell contact inhibits repair of potentially lethal damage (PLD) or that accumulation of additional lethal or sublethal damage during the post-thaw period for cells in contact is taking place. Pre- and post-FT trypsinization of single cells indicate that trypsin does not enhance repair of PLD caused by a FT cycle.
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D000818:Animals; D002450:Cell Communication; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology; D005615:Freezing; D007700:Kinetics; D008168:Lung; D014357:Trypsin / Q000494:pharmacology*
D014357:Trypsin; D004121:Dimethyl Sulfoxide