Enzymatic determination of red cell creatine as an index of hemolysis.
An enzymatic assay for creatine, depending on the creatine kinase reaction, has been modified for the determination of creatine in packed erythrocytes, using a centrifugal analyzer (COBAS BIO). The method is precise, sensitive and shows excellent accuracy in recovery experiments when compared to the diacetyl-alpha-naphthol method. The enzymatic red cell creatine correlates with the erythrocyte survival time determined with the radioactive chromium method. It can be used as a rapidly available parameter for the quantification of hemolytic processes.
Department of Clinical Chemistry, Inselspital, University of Berne, Switzerland.
H
H
Cramer
H
H
Dauwalder
H
H
Meier
J P
JP
Colombo
D000328:Adult; D000368:Aged; D000743:Anemia, Hemolytic / Q000097:blood / Q000175:diagnosis; D003401:Creatine / Q000097:blood*; D003402:Creatine Kinase / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006461:Hemolysis; D006801:Humans; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008875:Middle Aged; D009243:NAD / Q000097:blood; D011770:Pyruvate Kinase / Q000097:blood
D007202:Indicators and Reagents; D009243:NAD; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D003402:Creatine Kinase; D003401:Creatine