Cell-cooling in flow cytometry by Peltier elements.
We have built a cooling device for cell suspensions in flow cytometry that makes use of the Peltier effect (Barnard RD, Thermo electricity in Metals and Alloys, Taylor and Francis, London; Siemens-Z 34:383-88, 1963). The prototype described here is used for cooling collection tubes during long-duration cell sorting and is capable of maintaining a temperature of 2-5 degrees C in a cell suspension of up to 3 ml. In general, Peltier element-based cooling is useful for equilibrating the temperature of small volumes of fluids. Furthermore, Peltier element-based cooling devices are easy to build and handle.
Göttlinger
C
C
Meyer
K L
KL
Weichel
W
W
Müller
W
W
Raftery
B
B
Radbruch
A
A
D000818:Animals; D003080:Cold Temperature; D004553:Electric Conductivity; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D013816:Thermodynamics