An alternative fluorescence enhancement solution for use in lanthanide-based time-resolved fluoroimmunoassays.
Time-resolved fluoroimmunoassays that make use of lanthanide chelates as labels require the addition of an enhancement solution to elicit the formation of a fluorescent lanthanide complex. All solutions previously described are based on 2-naphthoyltrifluoroacetone (NTA), a beta-diketone. Currently, this compound is not commercially available. We report here the properties and performance of an enhancement solution prepared with a commercially available beta-diketone, thenoyltrifluoroacetone. Use of this solution in a commercial time-resolved fluoroimmunoassay gave results essentially identical to those obtained with the NTA-based solution, although fluorescence emission was approximately 27% lower. The lower fluorescence yield did not, however, significantly reduce assay sensitivity. We conclude that this solution represents a viable and highly economical alternative to the preparation currently in use, particularly for laboratories wishing to develop their own assays based on lanthanide fluorescence.
Department of Obstetrics and Gynaecology, National Women's Hospital, Auckland, New Zealand.
J A
JA
Keelan
J T
JT
France
P M
PM
Barling
D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D007986:Luteinizing Hormone / Q000097:blood; D008674:Metals, Rare Earth; D013050:Spectrometry, Fluorescence
D008674:Metals, Rare Earth; D007986:Luteinizing Hormone