[Relation between the dynamics of the cell cycle and the frequency of chromosome aberrations in cultured irradiated human lymphocytes].
The method of differential staining of sister chromatids was used to study the dynamics of cell divisions at different periods of fixation (48, 56, 68, 80 h) of human lymphocyte cultures in control and after gamma-irradiation in vitro in doses of 150 and 300 rad. It is shown that the cell population of lymphocytes is extremely asynchronous by the time of first and subsequent cell divisions. Administration of gamma-rays before PHA stimulation results in a mitotic delay whose duration is proportional to the irradiation dose. The frequency of radiation-induced chromosome aberrations does not reliably differ in early- and late-dividing cells.
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D002453:Cell Cycle / Q000528:radiation effects*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure; D013997:Time Factors
Vzaimosviaz' dinamiki kletochnykh tsiklov i chastoty khromosomnykh aberratsiĭ v kul'ture obluchennykh limfotsitov cheloveka.