Analysis of human blood for volatile N-nitrosamines by gas chromatography-chemiluminescence detection.
A method was developed to separate and measure trace levels of volatile N-nitrosamines (NAs) in human blood that either eliminated or accounted for in vitro artifactual formation of N-nitrosodimethylamine (NDMA) through the use of water blanks, added inhibitor (ascorbic acid) and added morpholine. The absolute minimum detectable limit was 8 pg; minimum level of reliable measurement was 0.05 microgram/kg for a 20-g blood specimen. Recovery of NDMA from blood was 93 +/- 5%. Coefficient of variation was 25%. Bloods from 242 people were analyzed for volatile NAs. NDMA was the only NA found. Positive specimens were presumptively confirmed by their non-detection after ultraviolet photolysis and/or mass spectrometry. This paper presents additional evidence that in vivo NA formation occurs.
Dunn
S R
SR
Pensabene
J W
JW
Simenhoff
M L
ML
D000328:Adult; D002849:Chromatography, Gas; D004128:Dimethylnitrosamine / Q000097:blood; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008297:Male; D009602:Nitrosamines / Q000097:blood*; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D009602:Nitrosamines; D004128:Dimethylnitrosamine