Application of a subcellular membrane-antibody binding assay for the analysis of antigen expression in human tumours.
A micro-radioisotopic antiglobulin assay has been employed to determine the binding of monoclonal antibodies to subcellular membranes adsorbed to the wells of Terasaki Microtest Plates. A panel of 15 monoclonal antibodies was tested against 22 membrane preparations isolated from normal and malignant colo-rectal, breast and lung tissues. The assay provided an objective evaluation of antibody reactivity permitting the expression of major antigens in samples from individual surgical specimens to be defined.
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MR
Price
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S
Edwards
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Baldwin
D000911:Antibodies, Monoclonal / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D001666:Binding Sites, Antibody; D001940:Breast / Q000276:immunology; D001943:Breast Neoplasms / Q000175:diagnosis*; D003106:Colon / Q000276:immunology; D003110:Colonic Neoplasms / Q000175:diagnosis*; D003298:Coombs Test / Q000295:instrumentation; D005260:Female; D006801:Humans; D007425:Intracellular Membranes / Q000276:immunology*; D008168:Lung / Q000276:immunology; D008175:Lung Neoplasms / Q000175:diagnosis*; D011863:Radioimmunoassay / Q000379:methods; D012004:Rectal Neoplasms / Q000175:diagnosis*; D012007:Rectum / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm