Subcellular distribution and selectivity of the protein-binding component of the recognition system for sex-hormone-binding protein-estradiol complex in human decidual endometrium.
In order to assess the subcellular distribution of the protein-binding component of the recognition system for sex-hormone-binding protein-estradiol complex (Strel'chyonok, O.A., Avvakumov, G.V. and Survilo, L.I. (1984) Biochim. Biophys. Acta 802, 459-466), plasma membranes and other subcellular fractions were prepared from homogenate of human decidual endometrium (8-12 weeks of pregnancy). Specific binding of 125I-labeled sex-hormone-binding protein complexed with estradiol was found preferentially in the plasma membrane fraction. The protein-binding component of the recognition system was found to specifically bind sex-hormone-binding protein complexed with estrogens (estradiol, estriol, estrone, with close affinities) but not with androgens (testosterone and 5 alpha-dihydrotestosterone). Specific membrane binding of 125I-labeled sex-hormone-binding protein complexed with 17 alpha-pregna-2,4-dien-20-yn-[2,3-d]isoxazol-17-ol (danazol) was also detected. This allowed us to suggest a putative mechanism for the danazol pharmacological action on endometrium which involves the interaction of the steroid-protein complex with the plasma membrane of endometrial cells. It is proposed that sex-hormone-binding protein takes part in the guided transport of steroids into endometrial cells.
Avvakumov
G V
GV
Zhuk
N I
NI
Strel'chyonok
O A
OA
D002458:Cell Fractionation; D003656:Decidua / Q000378:metabolism* / Q000648:ultrastructure; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006801:Humans; D007700:Kinetics; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012738:Sex Hormone-Binding Globulin / Q000302:isolation & purification / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D012738:Sex Hormone-Binding Globulin; D004958:Estradiol