Maximum activities of some key enzymes of glycolysis, glutaminolysis, Krebs cycle and fatty acid utilization in bovine pulmonary endothelial cells.
Despite the importance of endothelial cells little is known about their metabolic fuel requirements. To provide some information in this area, the maximum catalytic activities of key enzymes of important metabolic pathways have been measured in bovine pulmonary endothelial cells. The results suggest that both glucose and glutamine are important fuels for these cells: in addition, the oxidation of fatty acids may also be of quantitative significance. The activity of glutaminase in these cells was about 20-fold higher than that in lymphocyte, a cell which exhibits high rates of glutaminolysis. It is suggested that a high rate of glutamine metabolism by endothelial cells is important not only for energy provision but also for provision of nitrogen for biosynthetic purposes including production of local messengers.
Department of Biochemistry, University of Oxford, England.
B
B
Leighton
R
R
Curi
A
A
Hussein
E A
EA
Newsholme
D000818:Animals; D002384:Catalysis; D002417:Cattle; D002952:Citric Acid Cycle; D004730:Endothelium, Vascular / Q000201:enzymology*; D004734:Energy Metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005972:Glutaminase / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D006019:Glycolysis; D008214:Lymphocytes / Q000201:enzymology; D009584:Nitrogen / Q000378:metabolism; D011651:Pulmonary Artery / Q000201:enzymology; D051381:Rats
D005227:Fatty Acids; D005973:Glutamine; D005972:Glutaminase; D009584:Nitrogen