Role of glycosaminoglycans in calcium metabolism of human platelets.
Changes in intracellular Ca2+, [Ca2+]i, were measured in control and chondroitin ABC lyase-pretreated platelets. [Ca2+]i was measured with the fluorescent calcium probe Quin2. Chondroitin ABC lyase removed chondroitin 4-sulfate from the platelet surface without inducing shape change or release of serotonin. Compared to similarly prepared controls, enzyme treated platelets showed an increase of [Ca2+]i in response to stimulation by various agonists at high (1 mM) extracellular Ca2+ concentration. At low Ca2+ in the medium (1 mM EGTA), such platelets responded to agonists with a decreased rise in [Ca2+]i compared to the controls. These studies indicate that selective removal of glycosaminoglycans may sensitize platelets to the action of platelet aggregating agents. In addition, glycosaminoglycans may have a calcium storage function.
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D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002118:Calcium / Q000097:blood*; D002808:Chondroitin Lyases / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D006025:Glycosaminoglycans / Q000502:physiology*; D006801:Humans; D013917:Thrombin / Q000494:pharmacology
D006025:Glycosaminoglycans; D013917:Thrombin; D002808:Chondroitin Lyases; D002118:Calcium