Transport and intracellular localization of cortisol-asialotranscortin complexes in rat liver.
The influence of desialylation of human transcortin on transport of the transcortin-cortisol complex into the liver cells and its intracellular distribution was investigated in perfused rat liver. Under experimental conditions used the half-time of cortisol in perfusion medium was decreased more than 200 times in presence of asialotranscortin compared to that of native transcortin. Experiments with [3H]cortisol and [131I]asialotranscortin demonstrated a simultaneous uptake of cortisol and asialotranscortin by the hepatocytes. Distribution of [3H]cortisol and [131I]asialotranscortin in subcellular fractions showed the following pathway of cortisol-asialotranscortin complex: cell membrane, lysosomes, cytoplasm. The complex dissociates in lysosomes.
H
H
Karásková
K
K
Bezouska
L
L
Stárka
R
R
Hampl
A T
AT
Pikulev
M V
MV
Sholukh
O
O
Táborský
D000818:Animals; D001212:Asialoglycoproteins; D001692:Biological Transport; D006854:Hydrocortisone / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014156:Transcortin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D014316:Tritium
D001212:Asialoglycoproteins; D007457:Iodine Radioisotopes; C020707:asialotranscortin; D014316:Tritium; D014156:Transcortin; D006854:Hydrocortisone