Effect of cystic fibrosis and non-cystic fibrosis plasma on 45Ca and 35SO4 uptake and retention by the small intestine at the cellular level in the guinea pig.
A highly significant increase in net fluxes across the guinea-pig small intestine for both 45Ca2+ and 35SO4(2-), in the presence of cystic fibrosis plasma compared with non-cystic fibrosis plasma, was observed (p less than 0.001). In the presence of cystic fibrosis plasma, the nuclear, mitochondrial, microsomal and soluble fractions retained greater activity when compared with non-cystic fibrosis plasma. Of all the fractions, the nuclear fraction retained the highest activity both for 45Ca2+ and 35SO4(2-). These findings indicate that cystic fibrosis plasma increases the net fluxes and raises the retention of 45Ca2+ and 35SO4(2-) in guinea-pig small intestine at the cellular level.
S M
SM
Morrissey
J G
JG
Mehta
D000818:Animals; D001769:Blood; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D003550:Cystic Fibrosis / Q000097:blood*; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D007700:Kinetics; D013431:Sulfates / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D002132:Calcium Radioisotopes; D013431:Sulfates; D013462:Sulfur Radioisotopes; D002118:Calcium