[Specificity of interaction of hexokinase isozyme II with mitochondrial membranes].
Study on the mechanism of hexokinase isozyme II adsorption on mitochondrial membranes in the presence of 10 mM MgCl2 demonstrated that 0.16% of the total proteins of the soluble fraction and the total hexokinase pool are capable of reversible binding to the membrane. The plot for the dependence of the degree of enzyme adsorption on Mg2+ concentration is hyperbolic. Under these conditions, hexokinase competes favourably for the binding sites with lactate dehydrogenase and creatine kinase. Analysis of the adsorption capacity of natural and artificial phospholipid membranes showed that hexokinase isozyme II is adsorbed in much the same way on inner and outer mitochondrial membranes as well as on a mixture of membranes obtained from various sources and on lecithin liposomes. The adsorption properties of hexokinase isozyme II and of its functional analog--isozyme I--point to marked differences in the mechanism of their interaction with the membrane. In contrast with isozyme I, isozyme II of hexokinase undergoes kinetic alterations. Besides, it was found that mild autolysis of isozyme II is accompanied by a loss of the enzyme ability to bind to mitochondrial membranes. The data obtained suggest that the specificity of hexokinase isozyme II adsorption depends on the structural peculiarities of the protein but not on those of the mitochondrial membrane.
Muntian
E M
EM
Goncharova
N Iu
NIu
D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D006593:Hexokinase / Q000378:metabolism*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes; D008563:Membrane Lipids / Q000378:metabolism; D008929:Mitochondria, Heart / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000201:enzymology*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats
D007527:Isoenzymes; D008081:Liposomes; D008563:Membrane Lipids; D010743:Phospholipids; D006593:Hexokinase
O spetsifichnosti vzaimodeĭstviia izozima II geksokinazy s mitokhondrial'nymi membranami.