Purinergic modulation in the brain of the spiny lobster.
Earlier studies identified purinergic chemoreceptors in the olfactory organ of the spiny lobster, Panulirus argus. In this study, electrophysiological experiments demonstrate that purinergic substances can modulate both the spontaneous activity and the evoked responses of neurons within the brain of this animal. Perfusion of the brain with 100 microM adenosine 5'-monophosphate (AMP) modulated the spontaneous activity of 71% of the brain interneurons that were monitored. AMP also modulated the electrically or chemically evoked activity of 25% of the monitored interneurons. The effects were dose-dependent (down to 1 microM) and reversible. The modulatory effects of adenosine were similar to those of AMP, and were antagonized by the adenosine receptor antagonist 1,3-dipropyl-8-p-sulfophenylxanthine. The modulation by AMP or adenosine was depressive in most but not all neurons, as is the case with purinergic effects in the brain of vertebrates. We believe this is the first demonstration of modulatory effects of purinergic substances in the nervous system of any invertebrate.
Derby
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Department of Biology, Georgia State University, Atlanta 30303.
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Carr
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D000200:Action Potentials / Q000187:drug effects; D000241:Adenosine / Q000494:pharmacology* / Q000502:physiology; D000249:Adenosine Monophosphate / Q000494:pharmacology* / Q000502:physiology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008121:Nephropidae / Q000502:physiology*; D011983:Receptors, Purinergic / Q000187:drug effects / Q000502:physiology*; D014970:Xanthines / Q000494:pharmacology
D011983:Receptors, Purinergic; D014970:Xanthines; D000249:Adenosine Monophosphate; C053896:1,3-dipropyl-8-(4-sulfophenyl)xanthine; D000241:Adenosine