Spontaneous release of gamma-aminobutyric acid formed from putrescine and its enhanced Ca2+-dependent release by high K+ stimulation in the brains of freely moving rats.
The spontaneous release of [3H] gamma-aminobutyric acid ([3H]GABA) in various areas of rat brain injected with [3H]putrescine was examined using a push-pull perfusion technique. The release in a 25-min perfusate was highest in the caudate-putamen. The effect of high K+ stimulation on the release of [3H]GABA formed from [3H]putrescine was examined in the caudate-putamen. The release was enhanced by high K+ solution in a Ca2+-dependent manner.
T
T
Noto
H
H
Hashimoto
J
J
Nakao
H
H
Kamimura
T
T
Nakajima
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D011188:Potassium / Q000494:pharmacology*; D011700:Putrescine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D011188:Potassium; D002118:Calcium; D011700:Putrescine