Isolation and characterization of lectin-resistant mouse cell lines defective in their ability to yield hybrid cells.
Lectin-resistant variants of the mouse mammary tumor cell line FM3A in suspension were isolated after multiple-step selection using toxic concentrations of phytohemagglutinin (PHA) and concanavalin A (Con A). In fusion experiments, both PHA- and Con A-resistant lines were found to have lost the ability to yield hybrids with suspension-grown cells, although they were capable of yielding hybrids with monolayer-grown cells. This phenotype of PHA-resistant lines was stable during a long period of culture in the absence of PHA.
Matsuya
Y
Y
Yamane
I
I
D000818:Animals; D002448:Cell Adhesion; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D004351:Drug Resistance; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology*
D010835:Phytohemagglutinins; D003208:Concanavalin A