Effects of pure and auto-oxidized forms of cholesterol on plasma, liver lipids and hepatic lipogenesis in chicks.
Pure cholesterol (PC), oxidized cholesterol (OC) and cholestane-3 beta,5 alpha,6 beta-triol (CT) were fed to male Hubbard chicks for 3 weeks in a basal grain diet. Feed consumption and weight gain of chicks fed OC (1.0%) or CT (0.1%) were not significantly different from that of chicks fed PC (0.85%) or the basal diet alone. Plasma cholesterol level was significantly (P less than 0.05) higher in chicks fed PC compared to controls; however, compared to other dietary groups, differences were not significant. Measurement of hepatic lipogenesis, in vivo, from labeled substrates showed that [14C]acetate was primarily utilized for fatty acid synthesis in all dietary groups. The relative order was PC greater than PC + CT greater than OC greater than CT greater than basal. Conversely, [3H]mevalonate was preferentially used for cholesterogenesis and the relative effectiveness was basal greater than CT greater than OC greater than PC + CT greater than PC.
B H
BH
Cho
P O
PO
Egwim
G C
GC
Fahey
D000818:Animals; D002645:Chickens; D002777:Cholestanols / Q000494:pharmacology*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D007700:Kinetics; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000097:blood / Q000378:metabolism; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
D002777:Cholestanols; D002791:Cholesterol, Dietary; D000960:Hypolipidemic Agents; D010743:Phospholipids; D014280:Triglycerides; C000474:cholestane-3,5,6-triol; D002784:Cholesterol