The effect of ultraviolet, visible and infrared radiation on the rabbit blood-aqueous barrier.
We studied the effects of visible (400-750 nm), infrared (750-1100 nm), and ultraviolet (300-400 nm) radiation on the breakdown of the blood-aqueous barrier (BAB) in albino rabbit eyes using fluorophotometry and fluorescein-bound albumin. There was no evidence of BAB breakdown following exposure to visible (158 J cm-2) or infrared (106 J cm-2) radiation. Fluorescence was detected in the anterior chamber 15 min following ultraviolet (UV) (300-400 nm; 37 J cm-2 and 73 J cm-2) and ultraviolet-A (UV-A) (320-400 nm; 45 J cm-2 and 90 J cm-2) irradiation. This fluorescence peaked at 2 hr after irradiation. Reestablishment of the BAB, indicated by no detectable fluorescence, occurred in all animals after 24 hr. There was a significant increase in fluorescence with increasing UV-irradiation dosage in all groups (P less than 0.05). Comparison of BAB breakdown following exposure to ultraviolet 320-400 nm and 300-400 nm in the same dosage (45 J cm-2) indicated that the inclusion of shorter wavelength UV exposure produced a significantly higher degree of BAB breakdown (P less than 0.001).
Peyman
G A
GA
Fishman
P H
PH
Alexander
K R
KR
Woodhouse
M
M
Weinreb
R N
RN
D000818:Animals; D000867:Anterior Chamber / Q000528:radiation effects; D001082:Aqueous Humor / Q000528:radiation effects*; D001769:Blood / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005470:Fluorometry; D007259:Infrared Rays; D008027:Light; D011817:Rabbits; D012711:Serum Albumin, Radio-Iodinated / Q000378:metabolism; D014466:Ultraviolet Rays
D012711:Serum Albumin, Radio-Iodinated