[A method of determining inhibitory constants during reversible inhibition of enzymatic hydrolysis of a substrate].
Determination of inhibitory constants and antienzymic activity of reversible retardants of different type of action by the generalized inhibitory constant K sigma is estimated, results being presented. To determine more precisely the values of inhibitory constants of the competitive (Ki), noncompetitive (K'i) inhibition and of the generalized K sigma it is suggested to conduct linearization of the experimental data in coordinates: 1/K sigma, s; 1/[S], where K sigma, s is a total inhibitory constant whose value depends on the substrate [S] concentration and to make calculations by the least-square method with the allowance for principal intervals of the Km values and the maximal rate of the enzymic reaction V. Comparative calculations are made by a computer using the BASIC/RT-60 language programme through the example of the acetyl choline acetyl hydrolase (EC 3.1.1.7) interaction with the quaternary phosphonium salts--reversible retardants of this enzyme.
Brestkin
A P
AP
Zhukovskiĭ
Iu G
IuG
Rozengart
E V
EV
D004791:Enzyme Inhibitors; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008954:Models, Biological; D013379:Substrate Specificity
D004791:Enzyme Inhibitors
Metod opredeleniia ingibitornykh konstant pri obratimom tormozhenii fermentativnogo gidroliza substrata.