Effects of incubation temperature and bicarbonate on maturation of pig oocytes in vitro.
Pig oocytes cultured at 39 degrees C had a higher percentage of polar body formation than did those cultured at 37 degrees C. A culture medium based on Medium 199 with Earle's salts and supplemented with 15% serum from a young castrated boar was just as good as the same formulation containing additional pyruvate, lactate and insulin, and superior to a modified Krebs-Ringer bicarbonate medium. When the bicarbonate buffer system (Earle's salts) of Medium 199 was replaced with a phosphate buffer system (Hank's salts), the rate of polar body formation was decreased. When the Hank's based medium was supplemented with a bicarbonate buffer system, polar body formation was restored to the level in Earle's based medium. This suggests that CO2/bicarbonate may be important for the normal maturation of pig oocytes.
L A
LA
Eng
E T
ET
Kornegay
J
J
Huntington
T
T
Wellman
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D013552:Swine; D013696:Temperature
D001639:Bicarbonates; D003470:Culture Media