Radioimmunoassay of epitestosterone: methodology, thermodynamic aspects and applications.
A radioimmunoassay system for 17 alpha-hydroxy-4-androstene-3-one (epitestosterone) was developed and evaluated using rabbit antisera against 17 alpha-hydroxy-4-androstene-3-one-3-(O-carboxymethyl)-oxime-bovine serum albumin conjugate, and radioiodinated homologous histaminyl derivative of epitestosterone as a tracer. Two antisera with different specificity were used for radioimmunological determination of epitestosterone in hydrolyzed urine and plasma, respectively. Apparent intrinsic association constants and derived thermodynamic variables for ligand-antibody interaction were measured under various conditions in order to set up an optimal assay protocol. The mean plasma epitestosterone levels for healthy subjects were 0.44 +/- 0.15 nmol.l-1 (means +/- SD) in males and 0.092 +/- 0.056 nmol.l-1 in females. The corresponding concentrations in urine were 320 +/- 70 nmol.l-1 (males) and 273 +/- 100 nmol.l-1 (females).
Bílek
R
R
Research Institute of Endocrinology, Praha, Czechoslovakia.
Hampl
R
R
Putz
Z
Z
Stárka
L
L
D001826:Body Fluids / Q000032:analysis; D004845:Epitestosterone / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008297:Male; D011863:Radioimmunoassay; D013739:Testosterone / Q000032:analysis*; D013816:Thermodynamics; D013997:Time Factors
D007106:Immune Sera; D013739:Testosterone; D004845:Epitestosterone