DNA modified by platinum derivatives cannot adopt the A-form.
DNA fragments from chicken erythrocytes were modified by cis-diamminedichloroplatinum(II), its trans-isomer and chlorodiethylenetriaminoplatinum(II) chloride. The conformation of the modified DNA fragments in ethanolic solutions was studied by circular dichroism spectroscopy. Non-modified DNA adopted the A-form in 81% ethanol. The modification of DNA by the three platinum compounds inhibited the B to A transition of DNA induced by high concentrations of ethanol roughly to the same extent. The results support the view that the binding of the platinum complexes to B-DNA lowers the conformational freedom of DNA so that it cannot acquire the A-conformation.
Balcarová
Z
Z
Brabec
V
V
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002942:Circular Dichroism; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004912:Erythrocytes / Q000032:analysis; D000431:Ethanol; D009690:Nucleic Acid Conformation; D012996:Solutions
D012996:Solutions; C032799:chlorodiethylenetriamine platinum; C034697:transplatin; D000431:Ethanol; D004247:DNA; D002945:Cisplatin