[Detection and quantitative determination of lectins and viscotoxins in mistletoe preparations].
Mistletoe lectins and viscotoxins, which up to now have been isolated only from plant material, were detected and quantitatively determined in the mistletoe preparation Iscador and in a fermented mistletoe extract. Lectins were isolated by affinity chromatography and analyzed by isoelectric focussing. Thus, in Iscador and in the fermented mistletoe extract only the mistletoe lectins ML II/III were found whereas typical proteins of the ML I complex were missing. The lectin content of the preparation and the extract was determined by "single radial immunodiffusion" (SRID). For identification and quantitative determination of viscotoxins, a HPLC method was designed.
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Jordan
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D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D007525:Isoelectric Focusing; D037102:Lectins / Q000032:analysis / Q000302:isolation & purification*; D014784:Mistletoe / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D010946:Plants, Medicinal; D011863:Radioimmunoassay; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology
D037102:Lectins; D010936:Plant Extracts; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488862:mistletoe lectin I; C488863:mistletoe lectin II; C009674:ribosome inactivating protein, Viscum; C488038:viscotoxin; C077177:viscum album peptide; D054788:Ribosome Inactivating Proteins
Nachweis und quantitative Bestimmung von Lektinen und Viscotoxinen in Mistelpräparaten.