Methods for estimating the contribution of proenkephalin A and proenkephalin B input to neuronal areas.
Final expression of the proenkephalin A and proenkephalin B (prodynorphin) gene should yield a fixed ratio of enkephalin-like peptides depending on their precursor origin. If a tissue contained peptides only from proenkephalin A this ratio would be 4/1/1/1 (Met-Enk/Leu-Enk/Met-Enk-Arg6-Phe7/Met-Enk-Arg6-Gly7-Leu8). On the other hand, if the tissue-derived opiates were from prodynorphin, only Leu-enkephalin should be found. The techniques described here combining oxidation of the Met-Enk species, reverse-phase HPLC, and measurement with a radioimmunoassay which recognized many of the opiate-like peptides have been found useful for determining the precursor origin of enkephalins within different biological sources.
D K
DK
Sundberg
C E
CE
Dunlap
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions; D004745:Enkephalins / Q000032:analysis* / Q000235:genetics; D005796:Genes; D007106:Immune Sera; D007457:Iodine Radioisotopes; D009474:Neurons / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis* / Q000235:genetics; D011863:Radioimmunoassay / Q000379:methods; D014018:Tissue Distribution
D004745:Enkephalins; D007106:Immune Sera; D007457:Iodine Radioisotopes; D011498:Protein Precursors; C029992:proenkephalin