Purification and characterization of malignancy associated alpha-1 protein from lung tumor of a patient who died of primary carcinoma of the lung.
Malignancy associated ALPHA-1 protein(MAP) was isolated from a kilogram quantity of a lung tumor obtained at an autopsy of a patient who died of primary carcinoma of the lung. Acid extraction, Millipore filtration on a 30,000 molecular weight cut off membrane, treatment of the retentate with 50% saturated ammonium sulfate and gel filtration on Sephadex G-75 yielded a fairly pure material as judged by PAGE and RP-HPLC.
Krishnan
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D002277:Carcinoma / Q000032:analysis*; D006801:Humans; D008175:Lung Neoplasms / Q000032:analysis*; D009363:Neoplasm Proteins / Q000302:isolation & purification*
D009363:Neoplasm Proteins