5-Hydroxyeicosatetraenoate promotes Ca2+ and protein kinase C mobilization in neutrophils.
5-Hydroxyeicosatetraenoate (5-HETE) stimulated human neutrophils to elevate their cytosolic Ca2+. It also enhanced the actions of phorbol myristate acetate and a diacylglycerol in stimulating these cells to: a) translocate protein kinase C from cytosol to membranes; b) generate superoxide anion; and c) release granule-bound enzymes. 5,20-DiHETE and 15-HETE possessed little or none of these bioactions. 5-HETE may influence neutrophil function by elevating cytosolic Ca2+ and thereby promoting the mobilization of protein kinase C.
Department of Medicine, Wake Forest University Medical Center, Winston-Salem, North Carolina 27103.
J T
JT
O'Flaherty
J
J
Nishihira
D029742:Caenorhabditis elegans Proteins; D002118:Calcium / Q000097:blood*; D002352:Carrier Proteins; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D007700:Kinetics; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010100:Oxygen / Q000097:blood; D011493:Protein Kinase C / Q000097:blood* / Q000378:metabolism; D011955:Receptors, Drug; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism / Q000494:pharmacology
D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D006893:Hydroxyeicosatetraenoic Acids; D011955:Receptors, Drug; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen; D002118:Calcium