Detachment of ribosomes of rough endoplasmic reticulum by NADPH and reduced glutathione (GSH).
NADPH, an endogenous, naturally occurring compound was found to be positive in the stripping of ribosomes from rough endoplasmic reticulum (RER) test (degranulation) for carcinogens using the hepatocellular postmitochondrial (PMS) system. However, glutathione (GSH) another naturally occurring compound was negative in the hepatocellular PMS system in contrast to GSH which was previously reported as being positive in the mutagenicity test using kidney PMS but negative using liver PMS. Akintonwa has classified degranulation into three types, viz, DIA, DIB, and DII (D.A.A. Akintonwa, Ecotoxicol. Environ. Saf. 9, 53-63 (1985]. Most carcinogens fall into DIA and DIB types. NADPH was of DIA type in the mouse and of DII in the female rat PMS systems. NADPH was consistently negative in the male rat PMS system.
Akintonwa
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DA
D000818:Animals; D002273:Carcinogens; D004353:Drug Evaluation, Preclinical; D004721:Endoplasmic Reticulum / Q000187:drug effects*; D005260:Female; D005978:Glutathione / Q000633:toxicity*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009249:NADP / Q000633:toxicity*; D051381:Rats; D012270:Ribosomes / Q000187:drug effects*; D013045:Species Specificity
D002273:Carcinogens; D009249:NADP; D005978:Glutathione