Relationship between 201Tl uptake and oxidative metabolism in cultured myocardial cells.
The uptake of thallium-201 (201Tl) by myocardial cells in cultures was assessed in the presence of 10(-3) M potassium cyanide (KCN), an inhibitor of mitochondrial respiration, and 10(-4) M dinitrophenol (DNP), an uncoupler of oxidative phosphorylation. The cultures were incubated with 14C-glucose or 14C-octanoate, allowing the measurement of the oxidative metabolism and beta-oxidation from the production of 14CO2. The results demonstrated a moderate decrease in the ratio between the intra/extracellular concentration of 201Tl (Tl i/e) in the presence of KCN (28.9 +/- 8.1 versus 35.6 +/- 9.7 in the controls, n.s.) and no change with DNP (37.6 +/- 9.7). Glycolysis and fatty acid oxidation were lowered with KCN (-28 +/- 15 and -45 +/- 22% respectively, p less than 0.05 in both cases) and were non significantly increased with DNP (+37 +/- 23 and +10 +/- 52% respectively). These results show that 201Tl intracellular uptake is not related directly, but is not totally independent of glycolysis and fatty acid oxidation.
Gachon
P
P
Moins
N
N
Maublant
J C
JC
Ross
M R
MR
Davidson
W D
WD
Mena
I
I
D019297:2,4-Dinitrophenol; D002478:Cells, Cultured; D003470:Culture Media; D004140:Dinitrophenols / Q000494:pharmacology; D006321:Heart / Q000000981:diagnostic imaging; D006801:Humans; D066298:In Vitro Techniques; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011190:Potassium Cyanide / Q000494:pharmacology; D011868:Radioisotopes; D011877:Radionuclide Imaging; D013793:Thallium; D014475:Uncoupling Agents / Q000494:pharmacology
D003470:Culture Media; D004140:Dinitrophenols; D011868:Radioisotopes; D014475:Uncoupling Agents; D013793:Thallium; D011190:Potassium Cyanide; D019297:2,4-Dinitrophenol