Amount and type of leukocytes in 'leukocyte-free' red cell and platelet concentrates.
The exact number of leukocytes remaining in 'leukocyte-free' red cell and platelet concentrates cannot be measured by standard methods. We have therefore developed methods to harvest all the leukocytes from blood components. The leukocytes were then counted and identified using monoclonal antibodies. The leukocyte-free red cell concentrates were prepared by combining buffy coat removal and filtration through a Cellselect filter. The mean number of leukocytes per unit was 1.0 X 10(5). Most of the leukocytes were granulocytes and T cells. Only a few B cells or monocytes could be detected. Leukocyte-free platelets were prepared by filtering 4 units of PC through a cotton-wool (Imugard) filter. The mean number of leukocytes per PC unit was 0.4 X 10(5) of which 85-95% were T cells.
FRC Blood Transfusion Center, Helsinki, Finland.
J
J
Vakkila
G
G
Myllylä
D001773:Blood Cells; D002469:Cell Separation / Q000379:methods*; D017707:Erythrocyte Transfusion; D006801:Humans; D007518:Isoantibodies / Q000096:biosynthesis; D017708:Leukocyte Transfusion; D017713:Platelet Transfusion; D065227:Transfusion Reaction
D007518:Isoantibodies