A novel lipid-conjugated marker for the direct labeling of the schistosomulum membrane.
Introduction of the fluorescent group lissamine rhodamine B into the surface of schistosomula of S. mansoni was achieved by brief incubation of the worms with liposomes carrying the lipid bound fluorophore in their bilayers. The liposomes were made of egg lecithin (PC) and lissamine-phosphatidylethanolamine (lissamine-PE). The lissamine groups could be directly detected on the parasite membrane by observation of the single worms under a fluorescent microscope. The fluorescent marker was employed to measure the lateral diffusion coefficient (D) of lipids in the schistosomular membrane and as a surface marker during the isolation of the schistosomula surface membrane.
F
F
Levi-Schaffer
R
R
Tarrab-Hazdai
R
R
Arnon
M
M
Smolarsky
D000818:Animals; D000947:Antigens, Helminth; D000954:Antigens, Surface; D002462:Cell Membrane / Q000032:analysis / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D004058:Diffusion; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010714:Phosphatidylethanolamines; D012235:Rhodamines; D012550:Schistosoma mansoni / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure*
D000947:Antigens, Helminth; D000954:Antigens, Surface; D008563:Membrane Lipids; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; D012235:Rhodamines; C022027:lissamine rhodamine B