[Glio-neuronal aggregates in collagen gel: a model for studying the factors affecting development of central nervous system cells].
Glio-neuronal aggregates of dissociated cells obtained from embryonic brain during cultivation in rotating flasks were transfered for further cultivation into collagen gel, containing balanced salt solution, serum-free amino acid medium, or complete nutrient medium. Active neurite growth and glial cell migration were observed during cultivation in collagen gel. The experiments have shown that glio-neuronal aggregates may serve as an experimental model for testing the activity of different neuronotrophic and neurite growth stimulating factors.
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Viktorov
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Shashkova
D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development*; D002450:Cell Communication; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D004622:Embryo, Mammalian; D005782:Gels; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*
D003470:Culture Media; D005782:Gels; D003094:Collagen
Glio-neĭronal'nye agregaty v kollagenovom gele: model' dlia izucheniia faktorov, vliiaiushchikh na razvitie kletok tsentral'noĭ nervnoĭ sistemy.