Vasoactive intestinal peptide (VIP) binding to solubilized material from rat liver plasma membranes.
A non-ionic detergent such as Lubrol-PX extracts in soluble form the VIP-binding structures of rat liver plasma membranes. Detergent-solubilized proteins bind specifically [125I]VIP and the complex tracer-protein is identified by the use of Sepharose 6B columns. The interaction is only possible in the absence of detergent (below 0.001%) and is inhibited by native peptide. A molecular weight of about 80,000 was estimated for VIP-binding proteins by reference to a series of globular markers of proteins. Binding to VIP soluble proteins is specific and dependent on time as studied by the Hummel and Dreyer (Biochim. Biophys. Acta 63:530-532, 1962) assay.
J M
JM
Guerrero
J R
JR
Calvo
M R
MR
Garrido
P
P
Molinero
C
C
Osuna
J
J
Jiménez
R
R
Goberna
D000042:Absorption; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002850:Chromatography, Gel; D003902:Detergents / Q000494:pharmacology; D007457:Iodine Radioisotopes; D007700:Kinetics; D008099:Liver / Q000648:ultrastructure*; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats; D012995:Solubility; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism* / Q000494:pharmacology
D003902:Detergents; D007457:Iodine Radioisotopes; D014660:Vasoactive Intestinal Peptide