Sensitive assay of methadone in plasma by using capillary gas chromatography with photoionization detection.
A new gas chromatographic assay for methadone, utilizing a fused-silica capillary column, is presented. Extreme sensitivity was reached, compared to nitrogen-phosphorus and mass spectrometry detection, by employing a photoionization detector. Plasma concentrations of methadone as low as 1 ng/ml can easily be detected and, by further optimization, 0.1 ng/ml was reached. The minimum detectable amount of methadone reaching the detector was 70 fg. The results indicate that the photoionization detector has potential as a tool in drug monitoring.
Norlander
B
B
Carlsson
B
B
Bertler
A
A
D002849:Chromatography, Gas; D006801:Humans; D013058:Mass Spectrometry; D008691:Methadone / Q000097:blood*
D008691:Methadone