Kinetic model of glucose-6-phosphate dehydrogenase from red blood cells. Parameter estimation from progress curves and simulation of regulatory properties.
A kinetic model of human and mouse glucose-6-phosphate dehydrogenase is presented which takes into account the substrates and all inhibitors of significant importance in the red cell. The parameter values were estimated by analysis of progress curves. The applicability of a new method based on non-linear regression to complex enzyme kinetics was proved. The in vivo-regulation of glucose-6-phosphate dehydrogenase is examined by determining elasticity coefficients and by using simple simulation experiments. The model is convenient to describe the behaviour of enzyme activity under physiological conditions.
Buckwitz
D
D
Schönian
G
G
Holzhütter
H G
HG
Jacobasch
G
G
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood* / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D051379:Mice; D009249:NADP / Q000378:metabolism
D004163:Diphosphoglyceric Acids; D009249:NADP; D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase