Evaluation of a commercial cytotoxicity assay for detection of Clostridium difficile toxin.
A comparative study was performed to determine the accuracy of Clostridium difficile toxin detection. A commercial cytotoxicity assay (Bartels Immunodiagnostic Supply, Bellevue, Wash.) was compared with conventional microcytotoxicity assays, using Vero and MRC-5 cells. The Bartels system was found to be essentially equivalent to conventional cytotoxicity assays currently being performed for routine C. difficile toxin detection.
Nachamkin
I
I
Lotz-Nolan
L
L
Skalina
D
D
D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003013:Clostridium / Q000032:analysis*; D006801:Humans; D009500:Neutralization Tests; D011933:Reagent Kits, Diagnostic / Q000592:standards
D001427:Bacterial Toxins; D011933:Reagent Kits, Diagnostic