Monoclonal antibody (II2C) to human lactoferrin inactivates the myelopoietic suppressive effect of human lactoferrin in vitro.
A mouse monoclonal antibody was prepared against purified and fully iron-saturated human breast milk lactoferrin (LF). This antibody was of the IgG1 subclass, and recognized LF biosynthesized in low-density normal human bone marrow cells and LF stored in normal human polymorphonuclear neutrophils. This antibody did not recognize purified and iron-saturated human transferrin. The antibody inactivated the suppressive effects of purified and iron-saturated human milk LF and LF present in crude extracts of normal polymorphonuclear neutrophils against the release of granulocyte-macrophage colony-stimulating factors from mononuclear blood leukocytes in vitro.
G W
GW
Sledge
D C
DC
Bicknell
E L
EL
Harris
G
G
Zegarra
H E
HE
Broxmeyer
D000818:Animals; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000254:growth & development; D001854:Bone Marrow Cells; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D007108:Immune Tolerance; D007781:Lactoferrin / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D007782:Lactoglobulins; D007781:Lactoferrin