Allergens in pollen from mugwort (Artemisia vulgaris L.). II. Characterization of a crude and a partly purified extract of mugwort pollen with particular emphasis on the glycoprotein allergen Ag7.
A crude and a partly purified extract of mugwort pollen were characterized with particular emphasis on the glycoprotein allergen Ag7. Crossed immunoaffinoelectrophoresis with immobilized Con A in the intermediate gel demonstrated binding of Ag7 to this lectin, indicating that Ag7 contains terminal alpha-D-mannopyranosyl and/or alpha-D-glucopyranosyl residues. Crossed immunoaffinoelectrophoresis with free Con A in the first-dimension gel demonstrated microheterogeneity in the carbohydrate moiety of the allergen. Crossed radioimmunoelectrophoresis analysis using sera from 26 mugwort-allergic patients indicated that Ag7 is an important allergen in mugwort pollen. Heat stability experiments demonstrated the presence of at least three heat-resistant allergens, one of these being Ag7. Crossed immunoelectrofocusing indicated a pI value for Ag7 of 4.3.
B M
BM
Nilsen
B S
BS
Paulsen
D000485:Allergens / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003208:Concanavalin A / Q000494:pharmacology; D004355:Drug Stability; D006023:Glycoproteins / Q000032:analysis*; D007122:Immunoelectrophoresis / Q000379:methods; D007123:Immunoelectrophoresis, Two-Dimensional; D007525:Isoelectric Focusing; D010936:Plant Extracts / Q000032:analysis; D037121:Plant Lectins; D011058:Pollen / Q000302:isolation & purification*; D012685:Sepharose / Q000494:pharmacology
D000485:Allergens; D006023:Glycoproteins; D010936:Plant Extracts; D037121:Plant Lectins; D003208:Concanavalin A; D012685:Sepharose