Immunosuppressive activity of 15-deoxyspergualin and its effect on skin allografts in rats.
The immunosuppressive activity of spergualin analogues, 15-deoxyspergualin and N-30, is presented. These compounds suppressed antibody formation and establishment of delayed-type hypersensitivity to sheep red blood cells (SRBC) in mice. Among spergualin, 15-deoxyspergualin, and N-30, 15-deoxyspergualin was most effective in suppressing immune responses. It suppressed antibody formation against SRBC by spleen cell cultures in a dose-dependent fashion without reducing cell viability. The spergualins also suppressed the mixed lymphocyte culture reaction. Peritoneal exudate cells taken from mice given immunosuppressive doses of 15-deoxyspergualin were not reduced in their functions measured: Release of lysosomal enzymes and production of superoxide anions. 15-Deoxyspergualin and N-30 were markedly effective in prolonging skin graft in rats.
Institute for Chemotherapy, Microbial Chemistry Research Foundation, Shizuoka, Japan.
T
T
Masuda
S
S
Mizutani
M
M
Iijima
H
H
Odai
H
H
Suda
M
M
Ishizuka
T
T
Takeuchi
H
H
Umezawa
D000818:Animals; D000917:Antibody Formation; D005260:Female; D006085:Graft Survival / Q000187:drug effects; D006146:Guanidines / Q000276:immunology* / Q000627:therapeutic use; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000517:prevention & control; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000276:immunology*; D013154:Spleen / Q000187:drug effects
D006146:Guanidines; C054475:N 30; C033797:spergualin; C037258:gusperimus