Use of dantrolene plus multiple pulses to detect stress-susceptible porcine muscle.
Twitch characteristics of tibialis anterior muscles in situ were examined in stress-susceptible and normal swine. Three groups of pigs were studied: (1) purebred Pietrain stress-susceptible, (2) purebred Yorkshire normal, and (3) a crossbred (Pietrain-Yorkshire) litter containing both stress-susceptible and normal animals. Purebred and crossbred stress-susceptible pigs provided qualitatively similar results, as did purebred and crossbred normal pigs. Single stimuli produced greater than normal peak tensions and faster rates of tension development in stress-susceptible animals. Multiple stimuli (2-6 pulses at 5-ms intervals) increased peak tensions and rates of tension development, but did not augment differences between normal and stress-susceptible pigs. Intravenous administration of dantrolene reduced peak tensions and rates of tension development in all groups for single and multiple stimuli. However, the reduction was significantly less (P less than 0.01) for stress-susceptible pigs. Multiple stimuli (4-6 pulses) plus dantrolene amplified differences (P less than 0.01) in contractile properties between normal and stress-susceptible skeletal muscles, with stress-susceptible muscles obtaining larger peak tensions and faster rates of tension development. Normal and stress-susceptible pigs may, therefore, be distinguished by these procedures.
J G
JG
Quinlan
P A
PA
Iaizzo
G A
GA
Gronert
S R
SR
Taylor
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003620:Dantrolene / Q000494:pharmacology*; D004558:Electric Stimulation; D008305:Malignant Hyperthermia / Q000209:etiology / Q000503:physiopathology; D009119:Muscle Contraction / Q000187:drug effects*; D009132:Muscles / Q000503:physiopathology; D013312:Stress, Physiological / Q000209:etiology / Q000503:physiopathology*; D013552:Swine
D003620:Dantrolene; D002118:Calcium