Effects of neuron-conditioned medium and fetal calf serum content on glial growth in dissociated cultures.
The influence of the microenvironment as assessed by medium conditioned by 6-day-old chick embryo neurons in culture and of the nutrients derived from fetal bovine serum was evaluated in cultures of primary chick embryo glial cells. Glia-enriched cultures from 15-day-old chick embryo were incubated from culture days 3-9 with various concentrations of neuron-conditioned medium, with or without 10% fetal bovine serum in the final culture medium. Also, glial growth was studied in cultures with 5%, 10% or 20% fetal bovine serum in the medium. Glutamine synthetase and 2',3',-cyclic nucleotide 3'-phosphohydrolase were used as astrocytic and oligodendrocytic markers, respectively. Cultures were harvested at day 9. The presence of neuron-conditioned medium in the cultures was associated with persistence of immature glioblast-like cells. This persistence of glial immature cells was also reflected by the lower glutamine synthetase activity in the cultures with neuron-conditioned medium as compared to cultures with neuron-conditioned medium and fetal calf serum. In cultures with 5% neuron-conditioned medium without fetal bovine serum, cyclic nucleotide phosphohydrolase activity was increased. We are assuming that the input of neurons to the microenvironment is partially mediated through the neuron-conditioned medium. Thus, the present findings show that neurons influence the growth and differentiation of glial cells in culture.
N
N
Sakellaridis
D
D
Mangoura
A
A
Vernadakis
D000818:Animals; D001253:Astrocytes / Q000502:physiology; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D007106:Immune Sera / Q000494:pharmacology*; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009836:Oligodendroglia / Q000502:physiology
D007106:Immune Sera