Identification of 2',3'-cyclic nucleotide 3'-phosphodiesterase in bovine adrenal medulla.
The adrenal medulla contains an enzyme which catalyzes the hydrolysis of 2',3'-cAMP to 2'-AMP. For the parameters which have been examined, the adrenal medulla 2',3'-cAMP phosphodiesterase appears to be similar to brain 2',3'-cyclic nucleotide 3'-phosphodiesterase (also commonly referred to as CNPase). The apparent Km of the adrenal medulla CNPase for 2',3'-cAMP is 0.88 mM. The enzyme activity is unaltered by either EDTA, MgCl2 or CaCl2 in the presence or absence of calmodulin. The apparent molecular weight is 102,500 daltons. The function of the enzyme in either the brain or the adrenal medulla is, at the present time, unknown.
J G
JG
Tirrell
C J
CJ
Coffee
D000313:Adrenal Medulla / Q000201:enzymology*; D000818:Animals; D002147:Calmodulin / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D002458:Cell Fractionation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013347:Subcellular Fractions / Q000201:enzymology
D002147:Calmodulin