Cobra nerve growth factor: structure and evolutionary comparison.
We have cloned and sequenced an NGF cDNA from the cobra venom gland. The structure of the predicted cobra precursor resembles that of the mouse prohormone, with a highly conserved mature NGF protein at the C-terminus. In the non-NGF moiety, a putative signal peptide and two sets of basic residues presumably reflecting cleavage sites that are more conserved, while large interstitial regions are poorly conserved. This moiety may be involved in defining the folding of the precursor for subsequent proteolytic maturation and/or encode a bioactive polypeptide(s). Primer extension analysis indicates a single NGF transcript in the cobra venom gland.
Selby
M J
MJ
Hormone Research Institute, University of California, San Francisco 94143.
Edwards
R H
RH
Rutter
W J
WJ
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004546:Elapid Venoms / Q000032:analysis*; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000032:analysis*; D013045:Species Specificity
D004546:Elapid Venoms; D009414:Nerve Growth Factors; D004247:DNA