Sidestream cigarette smoke-exposure of mouse cells induces cell stress/heat shock-like proteins.
Specific alterations in cellular protein synthesis have been identified in mouse L929 and B16 cells exposed to "passive" (sidestream) smoke freshly generated from unfiltered cigarettes. A decrease in both cell viability and protein synthesis was observed in monolayer cell cultures following exposure to increasing numbers (0-12) of puffs of sidestream smoke. With exposures that resulted in approximately 30% or higher loss in cell viability, there was an apparent induction of cell stress/heat shock-like polypeptides with approximate molecular weights of 88,000, 66,000 and 23,000. After exposure to higher numbers of puffs that led to a loss of cell viability of 80% or greater, a different set of polypeptides was synthesized, including a major new protein of 38,000 mol. wt and 2 other predominant proteins of 45,000 and 30,000 mol. wt. The same specific effects on cellular protein synthesis were also observed after exposure to a similar number of puffs of the gas phase of sidestream cigarette smoke (minus the particulate phase components).
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D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005740:Gases; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D007739:L Cells; D007930:Leucine / Q000378:metabolism; D008545:Melanoma; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D005740:Gases; D006360:Heat-Shock Proteins; D010455:Peptides; D014028:Tobacco Smoke Pollution; D007930:Leucine