Molecular species analysis of lysophospholipids using high-performance liquid chromatography and argentation thin-layer chromatography.
Separation of the dimethyl ester derivative of lysophosphatidic acid into component molecular species was performed. This approach permits assessment of the metabolism of species of a given lysophospholipid class in studies that employ in vivo labeling where fatty acid analysis by gas chromatography is not useful. The relative utility of argentation thin-layer chromatography compared to reversed-phase high-performance liquid chromatography in biologically relevant model systems is discussed. Separation of 1,acyl- from 2,acyl- isomers of methylated lysophosphatidic acid was accomplished as was the analysis of monoglycerides by enzymatic radioactive phosphorylation.
Kennerly
D A
DA
University of Texas Health Science Center, Dallas 75235.
D001345:Autoradiography; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D007536:Isomerism; D008246:Lysophospholipids / Q000032:analysis*; D010761:Phosphorus Radioisotopes
D008246:Lysophospholipids; D010761:Phosphorus Radioisotopes