Electrophoretic analyses of secreted human endometrial proteins: identification and characterization of luteal phase prolactin.
Endometrial protein synthesis and secretion throughout the menstrual cycle was studied by slab gel electrophoretic analysis of [35S]methionine incorporation into protein during short term culture of human endometrial tissue. A minimum of five protein bands that fluctuate during the menstrual cycle were identified on one-dimensional gels. Those with mol wt of 28K, 35K, 51K, and 59K decreased in the luteal phase, whereas a broad 25K band was induced in the luteal phase. This broad band was identified as two species of glycosylated PRL by antihuman PRL immunostaining and [3H]glucosamine incorporation.
L J
LJ
Heffner
D A
DA
Iddenden
C R
CR
Lyttle
D000328:Adult; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000032:analysis*; D005260:Female; D006801:Humans; D008183:Luteal Phase; D008597:Menstrual Cycle; D010779:Photofluorography; D011388:Prolactin / Q000032:analysis*
D011388:Prolactin; D003101:Collodion