Primary structure of a major sialyl-saccharide alditol from human amniotic mucins expressing the tumor-associated sialyl-X antigenic determinant.
A major sialyl-saccharide alditol isolated from mucins of human amniotic fluid was purified by high-performance liquid chromatography followed by high-performance thin-layer chromatography of the methylated derivative and subjected to structural analyses which comprised fast atom bombardment and electron impact-mass spectrometry, methylation analysis, exoglycosidase digestion and CrO3 oxidation. The structure of a disialylated pentasaccharide characterized by a sialyl-X antigenic determinant was established: (formula; see text)
F G
FG
Hanisch
H
H
Egge
J
J
Peter-Katalinic
G
G
Uhlenbruck
D000653:Amniotic Fluid / Q000032:analysis*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D009077:Mucins / Q000302:isolation & purification; D009844:Oligosaccharides / Q000032:analysis*; D011247:Pregnancy; D013402:Sugar Alcohols / Q000032:analysis*
D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols; C016735:sialooligosaccharides