Analysis of plasma histamine: a modification of the enzymatic isotopic assay.
The enzymatic isotopic analysis of histamine requires extraction and concentration of [3H]-1-methylhistamine from the reaction mixture. The assay was modified to include enzymatic reaction at 0 degrees C to reduce blank values and direct application of protein-free reaction mixture to a thin-layer chromatography plate for isolating of the product. The analysis was quantitative down to 100 pg/ml of histamine, adequate for monitoring plasma histamine content.
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D006632:Histamine / Q000097:blood*; D006637:Histamine N-Methyltransferase; D006801:Humans; D008722:Methods; D014316:Tritium
D014316:Tritium; D006632:Histamine; D006637:Histamine N-Methyltransferase