Determination of low levels of 4-fluorophenylalanine incorporation into proteins.
This paper describes a rapid method for the determination of low levels of 4-fluorophenylalanine biosynthetically incorporated into proteins. Precolumn derivatization with phenylisothiocyanate followed by reverse-phase high-performance liquid chromatography enables determination of the amino acid composition of the protein as well as accurate detection of a 1-3% substitution of phenylalanine by fluorophenylalanine.
Gamcsik
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins / Q000032:analysis*; D017879:Isothiocyanates; D010649:Phenylalanine / Q000031:analogs & derivatives*; D011817:Rabbits; D013861:Thiocyanates; D009764:o-Phthalaldehyde; D010135:p-Fluorophenylalanine / Q000032:analysis / Q000097:blood*
D000596:Amino Acids; D006454:Hemoglobins; D017879:Isothiocyanates; D013861:Thiocyanates; C005441:phenylisothiocyanate; D010649:Phenylalanine; D010135:p-Fluorophenylalanine; D009764:o-Phthalaldehyde