The chymotrypsin-like activity of human prostate-specific antigen, gamma-seminoprotein.
gamma-Seminoprotein (gamma-Sm) is a human prostate-specific antigen and a serine protease judging from the complete amino acid sequence which shows extensive homology with the kallikrein family. The enzymatic activity of gamma-Sm was defined as a chymotrypsin-like activity using reduced and S-3-(trimethylated amino)propylated lysozyme and insulin-oxidized A and B chains as substrates. The -Leu/Ser- peptide bond of lysozyme was rapidly hydrolyzed by gamma-Sm. gamma-Sm also hydrolyzed the -Phe/Glu- of lysozyme and the -Leu/Cys(SO3H)- of insulin B chain. Insulin A chain and arginyl- or lysyl-linkage of these proteins were not hydrolyzed by gamma-Sm at all.
Department of Legal Medicine, Kurume University School of Medicine, Fukuoka, Japan.
K
K
Akiyama
T
T
Nakamura
S
S
Iwanaga
M
M
Hara
D000596:Amino Acids / Q000032:analysis; D000941:Antigens; D000951:Antigens, Neoplasm; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008297:Male; D009113:Muramidase / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011467:Prostate / Q000201:enzymology / Q000276:immunology*; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000378:metabolism*; D012661:Semen / Q000032:analysis; D029589:Seminal Plasma Proteins
D000596:Amino Acids; D000941:Antigens; D000951:Antigens, Neoplasm; D010446:Peptide Fragments; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D009113:Muramidase; D002918:Chymotrypsin