Local blood flow and vascular permeability of autonomic ganglion-transplants in the brain.
The purpose of this study was to determine whether the blood vessels of transplanted neural tissue retain their functional characteristics. Quantitative autoradiography was used to measure local blood flow (F) with iodoantipyrine and the blood-to-tissue transfer constant (K) with alpha-aminoisobutyric acid in superior cervical ganglion (SCG) allografted to the surface of ventricle IV and into the cerebellum of the same rat. The F of the intraparenchymal grafts was slightly lower than that of the intraventricular grafts; F decreased between 1 and 4 weeks in SCG grafts at both sites. The permeability-surface area (PS) product of the microvessels and extraction fraction of AIB were calculated from these results and indicated restricted transvascular passage of the amino acid in both the in situ and grafted SCG. Surface area (S) and average length (L) of the microvessels were determined morphometrically and their permeability (P) was calculated from these data. Although K and PS decreased in the grafts compared to in situ SCG, a comparable decrease in S indicated that P was similar for the microvessels of both in situ and 1-week-old SCG transplants: 3.5-4.3 x 10(-6) cm/s. Between 1 and 4 weeks after transplantation, the P of the microvessels decreased to approximately 1.6-2.3 x 10(-6) cm/s without any change in S. Thus, the blood vessels of SCG grafts within or upon the brain initially retain the functional attributes of in situ SCG microvessels, but the average permeability of the graft microvessels decreases to approximately one half of the initial value by 4 weeks after transplantation.
Tsubaki
S
S
Laboratory of Neurobiology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20205.
Brightman
M W
MW
Nakagawa
H
H
Owens
E
E
Blasberg
R G
RG
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002552:Cerebral Ventricles / Q000098:blood supply; D002560:Cerebrovascular Circulation; D005725:Ganglia, Autonomic / Q000098:blood supply / Q000637:transplantation*; D005728:Ganglia, Sympathetic / Q000098:blood supply / Q000201:enzymology / Q000637:transplantation*; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D013997:Time Factors; D014184:Transplantation, Homologous
D000469:Alkaline Phosphatase