A weaker gelatin-binding affinity and increased glycosylation of amniotic fluid fibronectin than plasma fibronectin.
Human amniotic fluid fibronectin had different carbohydrate moieties from plasma fibronectin. Nearly 90% of glycopeptides released from amniotic fluid fibronectin was not bound by concanavalin A-Sepharose, whereas 75% of glycopeptides from plasma fibronectin was bound. Amniotic fluid fibronectin showed a significantly lower gelatin-binding affinity than plasma fibronectin at 25 degrees C. When the incubation temperature was lowered to 4 degrees C, no significant difference in this activity was found. Cell-attachment promoting activity of the two fibronectins was not significantly different.
Y
Y
Yamaguchi
M
M
Isemura
Z
Z
Yosizawa
M
M
Kan
A
A
Sato
D000653:Amniotic Fluid / Q000378:metabolism*; D001665:Binding Sites; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005353:Fibronectins / Q000097:blood / Q000378:metabolism*; D005780:Gelatin / Q000378:metabolism*; D006020:Glycopeptides / Q000378:metabolism; D006801:Humans; D011247:Pregnancy; D011485:Protein Binding
D005353:Fibronectins; D006020:Glycopeptides; D005780:Gelatin