Separation of sialyl-oligosaccharides by high-performance liquid chromatography. Application to the analysis of mono-, di-, tri- and tetrasialyl-oligosaccharides obtained by hydrazinolysis of alpha 1-acid glycoprotein.
High-performance liquid chromatography has been applied to the separation of isomers of mono-, di-, tri- and tetrasialylated oligosaccharides derived from alpha 1-acid glycoprotein by hydrazinolysis. The separation of the sialyl-oligosaccharides on the basis of their negative charges was carried out with quaternary amine-bonded silica. Within each class, the anionic oligosaccharides were fractionated on the basis of their net carbohydrate content on alkylamine-modified silica using a mobile phase consisting of a mixture of acetonitrile and potassium dihydrogen phosphate with 0.01% of 1,4-diaminobutane or 0.01% of tetraethylpentamine.
P
P
Cardon
J P
JP
Parente
Y
Y
Leroy
J
J
Montreuil
B
B
Fournet
D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006834:Hydrazines; D006868:Hydrolysis; D007202:Indicators and Reagents; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000302:isolation & purification*; D009961:Orosomucoid / Q000302:isolation & purification; D012794:Sialic Acids / Q000032:analysis; D013329:Structure-Activity Relationship
D006834:Hydrazines; D007202:Indicators and Reagents; D009844:Oligosaccharides; D009961:Orosomucoid; D012794:Sialic Acids; C016735:sialooligosaccharides; C029424:hydrazine; D019158:N-Acetylneuraminic Acid