Glucocorticoid-receptor activation in hypertrophied skeletal muscle.
This investigation was undertaken 1) to determine whether the increased glucocorticoid-receptor binding activities, observed in hypertrophied plantaris muscles, are associated with a reduced ability to undergo receptor activation and 2) to examine whether glucocorticoid-receptor complexes in hypertrophied muscles undergo a shift in the relative distribution of the two thermally activated receptor forms (termed binder II and corticosteroid binder IB) to a distribution that is found in slow-twitch or heart muscle types. Plantaris muscles of female adrenalectomized rats, enlarged by surgical removal of synergists, were 60% heavier and had higher glucocorticoid cytosol binding (125 +/- 14 vs. 79 +/- 8 fmol/mg protein) than these muscles of controls. Activation, which was quantitated by the ability of the steroid-receptor complex to bind to DNA, was similar in overloaded and control muscles (57 +/- 2 vs. 62 +/- 4%). Diethylaminoethyl-cellulose chromatography of activated receptors showed approximately 16% of the radioactivity appearing as binder II and 38% as binder IB in both hypertrophied and control muscles. These results show that although enlarged plantaris muscles are undergoing certain fast- to slow-twitch biochemical transformations, the activated glucocorticoid-receptor distribution does not shift to that observed in slow fibers.
Department of Physical Education, University of Illinois, Chicago 60680.
M T
MT
Falduto
S M
SM
Czerwinski
T T
TT
Kurowski
R C
RC
Hickson
D000315:Adrenalectomy; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D006984:Hypertrophy / Q000378:metabolism / Q000473:pathology; D066298:In Vitro Techniques; D009132:Muscles / Q000378:metabolism* / Q000473:pathology; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D014222:Triamcinolone Acetonide / Q000378:metabolism
D011965:Receptors, Glucocorticoid; D004247:DNA; D014222:Triamcinolone Acetonide