Crystallization of the complexes between M315 idiotope and its monoclonal anti-idiotopic antibody Fab fragment.
The Fab fragment of a monoclonal anti-idiotopic antibody against M315 has been isolated and its complexes with Fv and Fab' fragment of M315 have been crystallized by using poly(ethylene glycol) 6000 or ammonium sulfate. X-ray diffraction photographs showed that the crystal of the complex with Fv diffracts better than that with Fab'. The Fv-complexed crystal was shown to be tetragonal I4, with cell dimensions a = 152 A and c = 69 A, and to contain one complex molecule of about 75,000 molecular weight in the crystallographic asymmetric unit.
Matsuura
Y
Y
Institute for Protein Research, Osaka University, Japan.
Inaka
K
K
Kusunoki
M
M
Katsube
Y
Y
Sakato
N
N
Fujio
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D003460:Crystallization; D007140:Immunoglobulin Fab Fragments; D007130:Immunoglobulin Idiotypes; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007130:Immunoglobulin Idiotypes