Regional differences in phagocytosis by the corneal endothelium in the domestic cat.
The phagocytosis of IgG-coated latex microspheres by the central and peripheral regions of the corneal endothelium in the domestic cat was investigated by organ culture. The endothelium was exposed to 1.357 X 10(7) microspheres/mm2, and counts of ingested microspheres were made by scanning electron microscopy. Initial assessments of phagocytosis over 8-h and 24-h periods of culture gave extremely low levels of incorporation. Therefore, subsequently, a 48-h period of culture was adopted. Microsphere uptake levels per square millimeter over 48 h were as follows: peripheral endothelium 6,021 +/- 3,162; central endothelium 2,000 +/- 1,529; overall 4,011 +/- 3,294. The greater uptake of microspheres in the peripheral cornea was statistically significant (P less than 0.002, paired t-test). The involvement of microvilli in phagocytosis by corneal endothelial cells was proposed.
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SD
Carrington
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Grierson
D000818:Animals; D001696:Biomechanical Phenomena; D002415:Cats; D003315:Cornea / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D003470:Culture Media; D004727:Endothelium / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010587:Phagocytosis
D003470:Culture Media