Brain protein changes during development and sexual differentiation in the rat.
Subcellular fractions were prepared from the hypothalamus-preoptic area and the 'remainder of the brain' of intact male and female rats at 0, 8, 25 and 72 days of age. Proteins associated with each fraction were subjected to SDS-PAGE chromatography and stained with Coomassie Brilliant Blue. Developmental changes were found to occur in proteins associated with the soluble (14,600, 15,000, 29,900, 38,900 and 49,000 mol. wt), nuclear (40,000-50,000 and 13,800-16,000 mol. wt.), mitochondrial-lysosomal (49,000-52,000 mol. wt.) and microsomal (14,400, 20,000, 50,100, 56,900 and 130,000 mol. wt.) fractions. In addition, soluble proteins were greater in males than in females at days 0 (53,000-56,000 mol. wt.; probably tubulin) and 25 (14,600 and 15,000 mol. wt.). These changes in brain proteins probably reflect important structural and functional changes that occur during maturation and sexual differentiation of the brain.
H F
HF
Stanley
N M
NM
Borthwick
G
G
Fink
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development*; D002467:Cell Nucleus / Q000378:metabolism; D005260:Female; D007031:Hypothalamus / Q000254:growth & development; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011301:Preoptic Area / Q000254:growth & development; D051381:Rats; D011919:Rats, Inbred Strains; D012733:Sex Differentiation; D013347:Subcellular Fractions / Q000378:metabolism
D009419:Nerve Tissue Proteins