Purification of a kappa-carrageenase from marine Cytophaga species.
A mixture of extracellular carrageenases was isolated from the cell-free medium of a culture of marine Cytophaga sp. 1k-C783 grown on ZoBell 2216 E broth with 0.1% commercial carrageenan. A single active peak of kappa-carrageenase was separated and purified from the mixture by ammonium sulfate precipitation, ion-exchange chromatography, and Sephadex G-200 gel filtration chromatography. Molecular weight of the purified kappa-carrageenase was estimated as 100,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified kappa-carrageenase had pH optimum 7.6 and temperature optimum 25 C.
Department of Bacteriology, Faculty of Medicine, Kagoshima University.
G
G
Sarwar
S
S
Matayoshi
H
H
Oda
D001419:Bacteria / Q000201:enzymology*; D001426:Bacterial Proteins; D002845:Chromatography; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D012623:Seawater; D013696:Temperature; D014871:Water Microbiology
D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; C019846:kappa-carrageenase protein, Alteromonadaceae