Occurrence of a new enzyme catalyzing the direct conversion of NG,NG-dimethyl-L-arginine to L-citrulline in rats.
An enzyme concerned with the degradation of NG,NG-dimethyl-L-arginine to L-citrulline was investigated in rats. The enzyme purified from rat kidney catalyzed the direct conversion of NG,NG-dimethyl-L-arginine to L-citrulline with liberation of dimethylamine from the methylated guanidino moiety. The reaction required no co-factor and the maximum activity was obtained at pH 6.5. The enzyme was highly specific for NG,NG-dimethyl-L-arginine.
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
T
T
Ogawa
M
M
Kimoto
K
K
Sasaoka
D000581:Amidohydrolases; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D002956:Citrulline / Q000096:biosynthesis*; D005260:Female; D006867:Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains
D002956:Citrulline; C018524:N,N-dimethylarginine; D001120:Arginine; D006867:Hydrolases; D000581:Amidohydrolases; C059999:dimethylargininase