The separation of the antibody and antigen components of immune complexes formed during normal human pregnancy.
By the manipulation of the fractionation conditions of a series of protein A affinity columns it was possible to separate the antibody and antigen components of the immune complexes formed in the serum during normal human pregnancy. The two components were partially characterised in order to determine that they were separate biological entities. The IgG immunoglobulin component possessed antibody activity directed against the outer layer of the placenta, the syncytiotrophoblast. The antigen component was of apparent syncytiotrophoblast origin and consisted of a protein with two major polypeptides of Mr 63 kDaltons and 31 kDaltons.
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D000906:Antibodies / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D000941:Antigens / Q000276:immunology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005331:Fetomaternal Transfusion; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D008565:Membrane Proteins / Q000276:immunology; D011247:Pregnancy; D014327:Trophoblasts / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D007074:Immunoglobulin G; D008565:Membrane Proteins