3H-xylamine binds to presynaptic rat striatal membranes.
3H-xylamine (3H-XYL), an irreversible catecholamine uptake inhibitor, was incubated with rat striatal synaptosomes, and the membrane fraction was examined by fluorography of a sodium dodecyl sulfate-polyacrylamide gel. A number of peptides were labeled. To determine their location, the striatal dopaminergic presynaptic nerve terminals were destroyed by unilateral electrolytic lesions through the nigrostriatal fibers prior to 3H-XYL exposure. The 3H-XYL bound to membranes from lesioned striata was about 29% of that bound to control membranes, which is consistent with the 83% reduction in dopamine (DA) uptake and the 68% reduction in DA content in the lesioned tissue. The decrease in peptide-bound 3H-XYL paralleled the decrease in DA content, with the exception of a 45% decrease in binding to a 45K peptide. These data show that 3H-XYL binding is predominantly localized in the dopaminergic presynaptic nerve terminals of the striatum.
S D
SD
Cushing
S
S
Howard-Butcher
R A
RA
Gazzara
A A
AA
Lettes
E
E
Mulliez
A K
AK
Cho
D000818:Animals; D001665:Binding Sites; D003342:Corpus Striatum / Q000378:metabolism*; D003714:Denervation; D004298:Dopamine / Q000502:physiology*; D008565:Membrane Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D009588:Nitrogen Mustard Compounds / Q000378:metabolism*; D051381:Rats; D013570:Synaptic Membranes / Q000032:analysis / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009588:Nitrogen Mustard Compounds; C026131:xylamine; D004298:Dopamine