Expression of the cDNA encoding for mouse sperm-specific lactate dehydrogenase C in Chinese-hamster ovary cells.
The cloned cDNA encoding for mouse sperm-specific lactate dehydrogenase C (LDH-C) was inserted immediately downstream to the MMTV 5' LTR promoter, and it was shown to synthesize mouse LDH-C polypeptide in Chinese-hamster ovary cells. The mouse LDH-C subunit and the endogenous Chinese-hamster LDH-A subunit formed in vivo a heterotetrameric LDH-A3C1 isoenzyme, and this novel isoenzyme exhibited enzymic activity utilizing lactate as substrate.
Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
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Hou
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Sakai
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Li
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005815:Genetic Code; D007122:Immunoelectrophoresis; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008297:Male; D051379:Mice; D010957:Plasmids; D011995:Recombination, Genetic; D013094:Spermatozoa / Q000201:enzymology*
D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase