Association of polyene antibiotics with sterols.
Molecular interaction between amphotericin B and sterols in non-aqueous solutions was examined quantitatively by spectroscopic methods in order to support the view point that selectivity of amphotericin B is more pronounced in the presence of ergosterol than of cholesterol. The most likely association complexes between ergosterol and amphotericin B are 4:1, 6:1 stoichiometric complex when the concentrations of amphotericin B are 3.93 x 10(-4) M, 1.94 x 10(-4) M respectively. The presence of 3 beta-OH group is necessary but not enough for the association with amphotericin B. It appears that the extent of spectral change of amphotericin B induced by complexing sterol is greater for ergosterol than cholesterol.
Institute for Protein Research, Osaka University, Japan.
M
M
Kito
H
H
Akutsu
H
H
Sugeta
Y
Y
Kyogoku
D H
DH
Sohn
B S
BS
Yu
D000666:Amphotericin B; D002784:Cholesterol; D004875:Ergosterol; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D013261:Sterols
D013261:Sterols; D000666:Amphotericin B; D002784:Cholesterol; D004875:Ergosterol