Establishment of a Vero cell line persistently infected with African swine fever virus.
A Vero cell line persistently infected with African swine fever virus was established by infecting the cells in the presence of 10 mM NH4Cl (Vero-P cell line). The virus derived from the Vero-P cultures infected Vero cells, and virus titers were comparable to those obtained in Vero cells acutely infected with African swine fever virus. The structural proteins of the virus from Vero-P cells were similar to those of the virus produced in lytic infections. Virus production was low when the Vero-P cells were growing logarithmically and increased considerably in confluent cultures when lysis appeared in a fraction of the cell population.
Salas
J
J
Viñuela
E
E
D000358:African Swine Fever Virus / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002522:Chlorocebus aethiops; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000382:microbiology; D007497:Iridoviridae / Q000254:growth & development*; D007668:Kidney; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis; D015678:Viral Structural Proteins; D014776:Virus Cultivation
D003470:Culture Media; D014764:Viral Proteins; D015678:Viral Structural Proteins; D000643:Ammonium Chloride