Distributions of fluorescence decay times for parinaric acids in phospholipid membranes.
Analysis of fluorescence decay data for probes incorporated into model or biological membranes invariably requires fitting to more than one decay time even though the same probe exhibits nearly single-exponential decay in solution. The parinaric acids (cis and trans) are examples of this. Data are presented for both parinaric acid isomers in dimyristoylphosphatidylcholine membranes collected to higher precision than normally encountered, and the fluorescence decays are shown to be best described by a smooth distribution of decay times rather than by a few discrete lifetimes. The temperature dependence of the fluorescence decay reveals a clear shift in the distribution to longer lifetimes associated with the membrane phase transition at 23.5 degrees C. The physical significance is that fluorescence lifetime measurements appear to reflect a physical process with a distribution of lifetimes rather than several distinct physical processes.
James
D R
DR
Department of Chemistry, University of Western Ontario, London, Canada.
Turnbull
J R
JR
Wagner
B D
BD
Ware
W R
WR
Petersen
N O
NO
D004134:Dimyristoylphosphatidylcholine; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D007700:Kinetics; D008081:Liposomes; D008954:Models, Biological; D010713:Phosphatidylcholines; D013050:Spectrometry, Fluorescence / Q000379:methods
D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D008081:Liposomes; D010713:Phosphatidylcholines; C013641:parinaric acid; C028694:1-palmitoyl-2-oleoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine