Simultaneous autoradiographic and histochemical analysis of bone formed in vitro.
The simultaneous histochemical demonstration of alkaline phosphatase activity and autoradiographic demonstration of [3H]-thymidine uptake is valuable for study of bone cell kinetics in vivo or in vitro. By use of this technique, it has been possible to detect changes induced by a single dose of dexamethasone (10(-7) M) in the time course of alkaline phosphatase activity, the number of alkaline phosphatase-positive cells, and [3H]-thymidine labeling in bone formed in vitro.
H C
HC
Tenenbaum
C A
CA
McCulloch
K
K
Palangio
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000502:physiology; D002645:Chickens; D046508:Culture Techniques; D003907:Dexamethasone / Q000494:pharmacology; D006651:Histocytochemistry; D007700:Kinetics; D010012:Osteogenesis / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism
D003907:Dexamethasone; D000469:Alkaline Phosphatase; D013936:Thymidine