Intermolecular histone H4 interactions in core nucleosomes.
Chicken histone H4, labeled at methionine-84 with 1-N-pyrenyliodoacetamide, has been incorporated into a nucleosome-like particle with core length DNA and unmodified histones H2A, H2B, and H3. These synthetic nucleosomes exhibit properties very similar to those displayed by native particles and those labeled with other fluors. The emission spectrum of the pyrene-labeled nucleosome was characteristic of excited dimer (excimer) fluorescence, indicating that the single pyrene groups on the two H4 molecules are in close proximity in the reconstituted particle. Histone H4 was also labeled randomly at lysines with a group that contains two pyrene moieties separated by 12 A at most. Incorporation of this histone into nucleosome-like particles provides an excimer standard which does not depend on intermolecular interactions. The properties of the pyrene-containing nucleosome were examined as a function of ionic strength. It was found that the H4-H4 pyrene excimer fluorescence exhibited a cooperative disruption centered at 0.1 M NaCl which preceded increases in accessibility and environment polarity revealed by other fluors attached at the same site.
Chung
D G
DG
Lewis
P N
PN
D000818:Animals; D002645:Chickens; D003432:Cross-Linking Reagents; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D004912:Erythrocytes / Q000378:metabolism; D006657:Histones / Q000097:blood* / Q000302:isolation & purification; D007096:Imidoesters; D009707:Nucleosomes / Q000378:metabolism*; D009994:Osmolar Concentration; D011721:Pyrenes; D013050:Spectrometry, Fluorescence; D013388:Succinimides
D003432:Cross-Linking Reagents; D006657:Histones; D007096:Imidoesters; D009707:Nucleosomes; D011721:Pyrenes; D013388:Succinimides; C024244:dithiobispropionimidate; C049261:dipyrenylcystine N-hydroxysulfosuccinimidyl monoester; D004247:DNA