Limitations of the hemoglobin method for detecting lipid hydroperoxides.
The method using peroxidase activity of hemoglobin (Hb) for the determination of lipid peroxides, trilinoleoylglycerol hydroperoxides and phosphatidylcholine hydroperoxides as substrates and tetramethyl benzidine as electron donor for the peroxidase reaction of Hb. The reactivities of these substrates were different. Some electron donors were tested for peroxidase activity of Hb, but none showed a complete reduction of methyl linoleate hydroperoxides. From these results, the Hb method needs to be carefully applied to biological materials that contain mixtures of different types of lipid classes.
Research Institute for Food Science, Kyoto University, Japan.
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Matsushita
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Terao
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Shibata
D000818:Animals; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008054:Lipid Peroxides / Q000032:analysis*; D010544:Peroxidases / Q000032:analysis; D013858:Thiobarbiturates
D006454:Hemoglobins; D008054:Lipid Peroxides; D013858:Thiobarbiturates; D010544:Peroxidases; C029684:thiobarbituric acid