Simple step gradient elution of the major high-energy compounds and their catabolites in cardiac muscle using high-performance liquid chromatography.
The majority of high-energy nucleotides and their catabolites are separated in a single 13-min run using reversed-phase high-performance liquid chromatography. Economical step gradient elution of these compounds with a Nova Pak-A C18, 5-microns, 10 cm X 8 mm column accomplishes this separation with recoveries of 94-100% and sensitivities of 1-5 pmol. Furthermore, the total adenine nucleotide pool can now be quantitated precisely in a simple and easily automated procedure.
E A
EA
Hull-Ryde
W R
WR
Lewis
C D
CD
Veronee
J E
JE
Lowe
D000227:Adenine Nucleotides / Q000302:isolation & purification / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D002021:Buffers; D002315:Cardiopulmonary Bypass; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006324:Heart Arrest, Induced; D009206:Myocardium / Q000378:metabolism*; D009711:Nucleotides / Q000032:analysis
D000227:Adenine Nucleotides; D002021:Buffers; D009711:Nucleotides; D000255:Adenosine Triphosphate