Chemoenzymatic synthesis of genes encoding medium-sized polypeptides by use of only one synthetic oligonucleotide.
A novel strategy for the synthesis of genes encoding medium sized polypeptides from only one synthetic oligodeoxynucleotide is outlined. A 140-mer oligodeoxynucleotide forming a hairpin structure at its 3'-end has been synthesized and successfully used in the construction and cloning of a gene coding for salmon calcitonin-gly(33). Employing this "one oligonucleotide - one gene" approach the manual work required for oligodeoxynucleotide synthesis is reduced to a minimum.
Uhlmann
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E
Hoechst AG, Frankfurt a.M. FRG.
Hein
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F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics*; D003001:Cloning, Molecular; D005796:Genes; D005813:Genes, Synthetic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010455:Peptides / Q000235:genetics*; D012474:Salmon
D009838:Oligodeoxyribonucleotides; D010455:Peptides; D002116:Calcitonin