Effect of aging on testicular epoxide hydrolase and glutathione-S-transferase activities in mice.
The effect of aging on epoxide hydrolase (EH) and glutathion-S-transferase (GST) activities was investigated in testes of C57BL/6 mice 1-30 months of age. Microsomal EH (mEH) activity, as monitored with cis-stilbene oxide (CSO), showed statistically insignificant changes throughout the lifespan of mice. Although cytosolic EH (cEH) was detected in testes by immunoblotting, the enzyme activity towards trans-stilbene oxide (TSO) could not be measured under the experimental conditions used. Gonadal GST monitored with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate displayed an increasing trend until the mice reached senescence, showing a 3.7-fold increase in the enzyme activity in old animals (30 months) when compared with that in young animals (2 months). However, with CSO as substrate, GST showed no change in activity in mice of different ages.
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D000375:Aging; D000818:Animals; D001835:Body Weight; D004591:Electrophoresis, Polyacrylamide Gel; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D013737:Testis / Q000201:enzymology*
D005982:Glutathione Transferase; D004851:Epoxide Hydrolases