Deleterious effects of fungizone on growth hormone and prolactin secretion by cultured GH3 cells.
The rates at which growth hormone (GH) and prolactin (PRL) are spontaneously secreted from a rat pituitary tumor cell line (GH3) were significantly reduced when these cells were maintained in medium containing 2.5 micrograms/ml Fungizone (Fz). The reduction in hormone secretion was not immediately reversed by removal of Fz during perifusion, but after 3 wk in control medium, secretory rates approached the pre-Fz treatment levels. In plated cells, secretion of GH was reduced by Fz in a dose-dependent manner, whereas PRL secretion was significantly reduced only by the highest concentration (2.5 micrograms/ml) of Fz. We concluded that Fz is not an acceptable medium constituent for the long-term culture of GH3 cells. However, because its effects are reversible, its short-term use as a decontaminating agent might eliminate the necessity for reinitiating the culture of cells whose secretory behavior must be followed in long-term protocols.
Lapp
C A
CA
Department of Medicine, Medical College of Georgia, Augusta.
Tyler
J M
JM
Stachura
M E
ME
Lee
Y S
YS
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D013006:Growth Hormone / Q000096:biosynthesis*; D009761:Nystatin / Q000494:pharmacology; D011388:Prolactin / Q000096:biosynthesis*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D009761:Nystatin; D000666:Amphotericin B; D011388:Prolactin; D013006:Growth Hormone