Nerve terminal remodeling visualized in living mice by repeated examination of the same neuron.
The distribution of presynaptic endings on the surfaces of autonomic ganglion cells was mapped in living mice after intravenous administration of a styryl pyridinium dye. The staining and imaging techniques did not appear to damage the ganglion cells, or the synapses on them; these procedures could therefore be repeated after an arbitrary period. Observations of the same neurons at intervals of up to 3 weeks indicate that the pattern of preganglionic terminals on many of these nerve cells gradually changes.
Purves
D
D
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.
Voyvodic
J T
JT
Magrassi
L
L
Yawo
H
H
D000200:Action Potentials; D000818:Animals; D004396:Coloring Agents; D005456:Fluorescent Dyes; D005726:Ganglia, Parasympathetic / Q000166:cytology* / Q000502:physiology; D008564:Membrane Potentials; D051379:Mice; D009411:Nerve Endings / Q000648:ultrastructure; D009473:Neuronal Plasticity; D011726:Pyridinium Compounds; D013997:Time Factors; D014741:Video Recording
C032315:4-(4-dimethylaminostyryl)-1-methylpyridinium; D004396:Coloring Agents; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds