Induction of S-adenosylmethionine synthetase isozymes in primary cultures of adult rat hepatocytes.
The activities of S-adenosylmethionine synthetase isozymes were studied using adult rat hepatocytes in primary culture. Hepatocytes from adult rats were isolated and cultured for several days. The activities of the synthetase isozymes did not change during primary culture. The activity of the alpha-form increased with increasing ethionine plus adenine or methionine in the medium, and reached about 5 fold after 2 days. However, the increased activity of the beta-form showed less than twice.
Sawai
Y
Y
Suma
Y
Y
Tsukada
K
K
D000225:Adenine / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005001:Ethionine / Q000494:pharmacology; D007527:Isoenzymes / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D008715:Methionine / Q000494:pharmacology; D008716:Methionine Adenosyltransferase / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014166:Transferases / Q000096:biosynthesis*
D007527:Isoenzymes; D008715:Methionine; D014166:Transferases; D008716:Methionine Adenosyltransferase; D000225:Adenine; D005001:Ethionine; D004121:Dimethyl Sulfoxide