Na+-coupled D-glucose uptake and membrane order of enterocyte brush border membrane vesicles, under the effect of a series of N-phenylcarbamates.
The importance of the hydrophobic effect of exogenous substances and of modifications of membrane order on D-glucose uptake are still poorly defined. Our results show that the concentrative Na+ -coupled D-glucose uptake of rat enterocyte brush border membrane vesicles is inhibited by N-phenylcarbamates increase the membrane order. However, since the concentrations required for membrane order increase are much greater than those active on D-glucose uptake, the effects on lipid order cannot be responsible for the inhibition of D-glucose uptake. Measurements of D-glucose uptake under conditions of Na+ equilibrium show that these carbamates do not act directly on the carrier but indirectly by favouring the dissipation of the Na+ gradient.
Y
Y
Fernandez
R A
RA
Boigegrain
C
C
Cambon-Gros
P
P
Deltour
S
S
Mitjavila
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002219:Carbamates / Q000494:pharmacology*; D002748:Chlorpropham / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008051:Lipid Bilayers; D008560:Membrane Fluidity / Q000187:drug effects; D008871:Microvilli / Q000378:metabolism; D048448:Phenylcarbamates; D051381:Rats; D012964:Sodium / Q000378:metabolism*
D002219:Carbamates; D008051:Lipid Bilayers; D048448:Phenylcarbamates; D002748:Chlorpropham; D012964:Sodium; D005947:Glucose; C100175:propham