Muscle activity pattern regulates postnatal development of acetylcholinesterase molecular forms in normal mice and mice with motor endplate disease.
We have studied the relative contributions of muscle activity and nerve-supplied materials to the regulation of AChE molecular forms during postnatal development of muscles in normal mice and in mice with motor endplate disease (med mice). Onset of this hereditary disease causes a progressive failure of evoked release of ACh from the motor neuron, which prevents contraction in muscles such as biceps and soleus. In these innervated but inactive muscles, one can examine the consequences of inactivity on the distribution of AChE forms. In normal mouse biceps the distribution of AChE forms, as shown by sucrose-gradient analysis, change substantially after birth; the most dramatic alteration is an increase in G4 AChE from 15 to 45% of total AChE during the third postnatal week. AChE profiles in normal or med biceps are indistinguishable until 10-12 d after birth, but the changes in distribution of AChE forms does not occur in med biceps nor in normal biceps denervated 2 weeks after birth. In contrast, the distributions of AChE forms in a predominantly slow muscle, the soleus, are similar in med and normal mice both early (10 d) and late (20 d) in the course of the disease, and the distributions are affected little by denervation. The profiles of AChE forms seen in normal soleus at all times studied resembled those seen in newborn biceps or biceps inactivated by denervation or the med disease. We conclude that neither innervation, age-dependent changes intrinsic to muscle, nor muscle activity is sufficient to induce the changes we seen in AChE forms in biceps. These results support the hypothesis that neonatal, inactive, or tonically active muscles produce an intrinsic pattern of AChE molecular forms, and that a phasic pattern of activity induces a postnatal redistribution of the AChE molecular forms expressed by the muscle.
Yeakley
J M
JM
Department of Biology, Pomona College, Claremont, California 91711.
Janavs
J L
JL
Reiness
C G
CG
D000110:Acetylcholinesterase / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D051379:Mice; D008817:Mice, Mutant Strains; D009045:Motor Endplate / Q000378:metabolism / Q000503:physiopathology*; D009132:Muscles / Q000294:innervation / Q000378:metabolism* / Q000502:physiology / Q000503:physiopathology; D009468:Neuromuscular Diseases / Q000378:metabolism / Q000503:physiopathology*; D009469:Neuromuscular Junction / Q000503:physiopathology*
D000110:Acetylcholinesterase