Structure of the extracellular gelling polysaccharide produced by Enterobacter (NCIB 11870) species.
The gelling polysaccharide produced by a species of Enterobacter (NCIB 11870) contains L-fucose, D-glucose, and D-glucuronic acid in the ratios 1:2:1. Analysis of the methylated and methylated, carboxyl-reduced polysaccharide revealed terminal non-reducing glucose, (1----3)-linked fucose, (1----3,1----4)-linked glucose, and (1----4)-linked glucuronic acid in the ratios 1:1:1.2:0.8. From the results of Smith degradation of the polysaccharide and spectroscopic studies of the acidic tetra- and octa-saccharides produced by bacteriophage-induced enzymic depolymerization of the polysaccharide, the following tetrasaccharide repeating-unit is proposed. (Formula: see text). This repeating-unit is identical to that of the capsular polysaccharide produced by Klebsiella aerogenes serotype K54 except for the absence of O-acetyl groups. The effects of the O-acetyl groups on the secondary structure and rheological properties of these polysaccharides are discussed.
M A
MA
O'Neill
V J
VJ
Morris
R R
RR
Selvendran
I W
IW
Sutherland
I T
IT
Taylor
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004754:Enterobacter / Q000276:immunology*; D004755:Enterobacteriaceae / Q000276:immunology*; D007202:Indicators and Reagents; D007709:Klebsiella / Q000276:immunology; D009844:Oligosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D013045:Species Specificity
D007202:Indicators and Reagents; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial