Effect of 3-aminobenzamide on the process of ultraviolet-induced DNA excision repair.
The effect of 3-aminobenzamide, a potent inhibitor of poly(ADP-ribosyl)ation, on UV-induced DNA excision repair was investigated. HeLa cells were treated with DNA replication inhibitors, hydroxyurea (HU) and 1-beta-D-arabinofuranosyl cytosine (araCyt), before and after ultraviolet light (UV) irradiation, to accumulate DNA single-strand breaks. The activity of poly(ADP-ribosyl)ation measured in the permeable cell system of HeLa cells was enhanced in a UV dose-dependent manner after the combined treatment with HU and araCyt in vivo. However, DNA repair synthesis in vitro was not affected by addition of 1 mM 3-aminobenzamide or nicotinamide, while incorporation of [3H]NAD in the same system was completely inhibited. Furthermore, neither the magnitude of UV-induced DNA single-strand breaks accumulated by the combined treatment of HU and araCyt nor the rate of their rejoining after release from the HU and araCyt block were influenced even in the presence of 10 mM 3-aminobenzamide. As the cytotoxicity of UV irradiation was significantly potentiated by 5 mM 3-aminobenzamide, these results suggest that poly(ADP-ribosyl)ation is involved in a process other than DNA excision repair induced by UV irradiation.
Otsuka
F
F
Yamada
K
K
Ohno
H
H
Enomoto
T
T
Hanaoka
F
F
Ohsawa
M
M
Yamada
M
M
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D001549:Benzamides / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003561:Cytarabine / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D004261:DNA Replication / Q000187:drug effects; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D014466:Ultraviolet Rays
D001549:Benzamides; D003561:Cytarabine; D000246:Adenosine Diphosphate Ribose; C025160:3-aminobenzamide; D006918:Hydroxyurea