Detection of clonal karyotypic abnormalities in most patients with acute nonlymphocytic leukemia examined using short-term culture techniques.
Cytogenetic studies using short-term culture methods, both with and without methotrexate synchronization, were performed on 53 consecutive patients with acute nonlymphocytic leukemia. Among 50 patients with adequate specimens, 42 (84%) showed a clonal karyotypic abnormality. Numerical changes were observed in 22 cases. Chromosome #8 was most frequently gained (eight cases), whereas chromosome #7 was most frequently lost (five cases). Structural rearrangements were detected in 35 cases. A 5q- was seen in three cases, 6q- in two, 7q- in four, standard or variant t(8;21) in four, 11p- in two, t(11q23;-) in two, t(15;17) in four, inv(16) in two, and inv(20) in two. In five patients small abnormal clones were observed, which represented less than 10% of the total cells analyzed. In five other patients the sole abnormality detected was a tiny structural rearrangement. There was no significant difference in remission rate or in survival among groups of patients without clonal abnormality, with a mixture of normal and abnormal karyotypes or with only abnormal cells. Patients who showed complex karyotypes (four or more chromosomal alterations) had a very poor prognosis. In contrast, patients with t(8;21) tended to have a favorable survival outlook. A normal karyotype was associated with an intermediate prognosis.
Misawa
S
S
Hogge
D E
DE
Oguma
N
N
Wiernik
P H
PH
Testa
J R
JR
D000208:Acute Disease; D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002999:Clone Cells; D005260:Female; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000145:classification / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D011379:Prognosis