Transplantation of dissociated foetal serotonin neurons into the transected spinal cord of adult rats.
Twenty adult rats underwent a complete section of the spinal cord at the lower thoracic level. One week later, 15 of them received a cell suspension obtained from raphe nuclei of 14-day-old foetuses into the distal fragment of the spinal cord. They were sacrificed after survival periods of 10-60 days, and vibratome sections of the spinal cord were processed for immunocytochemical detection of serotonin (5-HT). The control, non-transplanted animals showed a total absence of 5-HT immunoreactivity below the section, whereas the transplanted rats showed many immunoreactive 5-HT perikarya in the graft region, some at a distance of up to 10 mm, and a progressive innervation of the whole grey matter extending at least over 20 mm from the graft site.
A
A
Privat
H
H
Mansour
A
A
Pavy
M
M
Geffard
F
F
Sandillon
D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000637:transplantation; D006651:Histocytochemistry; D007120:Immunochemistry; D008297:Male; D009415:Nerve Net / Q000033:anatomy & histology; D009416:Nerve Regeneration; D009474:Neurons / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism / Q000601:surgery* / Q000648:ultrastructure
D012701:Serotonin