Overdrive is a T-region transfer enhancer which stimulates T-strand production in Agrobacterium tumefaciens.
Introduction of a left or right synthetic border repeat together with the overdrive sequence in an octopine Ti-plasmid deletion mutant, lacking the right border, resulted in the complete restoration of the oncogenicity of the mutant strain. However introduction of a border repeat without the overdrive, only restored oncogenicity partially. The overdrive sequence turned out to be able to stimulate the synthetic border mediated T-region transfer, independent of its orientation and position relative to the border repeat. Furthermore the distance between border repeat and overdrive could be enlarged, without a loss of overdrive activity. Here we enlarged the distance between the two sequences up to 6714bp. These results were confirmed by estimating the amount of single stranded T-DNA molecules from induced agrobacteria, containing the various border constructs.
Department of Plant Molecular Biology, Leiden University, The Netherlands.
M J
MJ
van Haaren
N J
NJ
Sedee
R A
RA
Schilperoort
P J
PJ
Hooykaas
D004742:Enhancer Elements, Genetic; D005822:Genetic Vectors; D010935:Plant Diseases; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic