A rapid and sensitive method for the identification and quantitation of sub-picomolar amounts of neurohypophysial peptides.
A simple, rapid and sensitive method, using isocratic reversed-phase HPLC, is described for the concomitant identification and quantitation of low levels of the various neurohypophysial peptides in biological tissues and fluids. The method requires little or no sample preparation and utilises UV peak detection at 215 nm with a serial signal amplification system to achieve a usable maximum sensitivity of less than 200 fmol of peptide.
Department of Dentistry, University of Adelaide, South Australia.
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D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006168:Guinea Pigs; D008832:Microchemistry / Q000379:methods; D010121:Oxytocin / Q000032:analysis; D010907:Pituitary Hormones / Q000032:analysis*; D011817:Rabbits; D051381:Rats
D010907:Pituitary Hormones; D001127:Arginine Vasopressin; D010121:Oxytocin