A new possibility to analyse urinary proteins: isoelectrofocusing with immobilized pH gradients.
As a new method for the analysis of proteinuria isoelectricfocusing with immobilized pH gradients is introduced. Ths new approach has several advantages over the conventional methods. The applicability is demonstrated by analysing the long-term changes in proteinuria of a 5/6 nephrectomized rat. Soon after 5/6 nephrectomy, a glomerular pattern of protein excretion predominates which later on is followed by a tubular pattern.
E
E
Meisinger
N
N
Gretz
M
M
Strauch
D000375:Aging; D000419:Albuminuria; D000818:Animals; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000652:urine; D007525:Isoelectric Focusing / Q000379:methods*; D009392:Nephrectomy; D011507:Proteinuria; D051381:Rats; D012177:Retinol-Binding Proteins / Q000652:urine; D014168:Transferrin / Q000652:urine; D014511:Uremia / Q000503:physiopathology
D007074:Immunoglobulin G; D012177:Retinol-Binding Proteins; D014168:Transferrin