Removal of glycosaminoglycans from bovine granulosa cells contributes to increased binding of hydrogen-3 heparin.
Granulosa cells from small or large bovine follicles were pretreated with enzymes that hydrolyze various glycosaminoglycans, and binding of [3H]-heparin to the granulosa was measured. Binding of [3H] heparin increased significantly after enzymatic pretreatments with chondroitinase ABC and fungal hyaluronidase, and similar results were obtained with granulosa from small and large follicles. No changes in binding of [3H] heparin were detected after hydrolyses with chondroitinase AC and heparinase in either follicle size. Heparitinase, which hydrolyzes heparan sulfate, led to a significant 50% increase in binding of [3H] heparin to granulosa from large follicles but was without effect in small follicles. These results suggest that the lower binding of [3H] heparin, which has been reported with follicular enlargement, may be due to heparan sulfate occupying or obstructing binding sites for heparin on granulosa from large follicles.
R L
RL
Ax
C M
CM
Stodd
S K
SK
Boehm
M E
ME
Bellin
D000818:Animals; D002417:Cattle; D002810:Chondroitinases and Chondroitin Lyases / Q000494:pharmacology; D005260:Female; D006025:Glycosaminoglycans / Q000037:antagonists & inhibitors*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D019763:Heparin Lyase; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D066298:In Vitro Techniques; D011133:Polysaccharide-Lyases / Q000494:pharmacology*
D006025:Glycosaminoglycans; D006493:Heparin; D006821:Hyaluronoglucosaminidase; D002810:Chondroitinases and Chondroitin Lyases; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase; C021349:heparitinsulfate lyase