In vitro transcription of a chromatin-like complex of major core protein VII and DNA of adenovirus serotype 2.
Major core protein VII of adenovirus serotype 2 (Ad2) is thought to play a role as a histone octamer in eukaryotic cells. We compared the template activity of the VII-DNA complex formed in vitro with that of protein-free DNA. Hybridization assay of in vitro transcripts showed that transcription from regions located in the middle of Ad2 DNA decreased when Ad2 DNA formed a complex with VII. This suggests that the chromatin structure plays a role in regulation of transcription of the adenovirus genome.
Y
Y
Nakanishi
K
K
Maeda
M
M
Ohtsuki
K
K
Hosokawa
S
S
Natori
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002843:Chromatin / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000378:metabolism*
D002843:Chromatin; D004279:DNA, Viral; D014758:Viral Core Proteins