Procedure for effective separation of high-density lipoproteins in normal serum and hypertriglyceridemic samples.
A chemical procedure for high-density lipoprotein (HDL) separation in normo- and hyperlipidemic sera has been developed. The procedure is effective up to 25 mmol/L of serum triglycerides and the constituents of the precipitation mixture (dextran sulfate, PEG-6000 and MgCl2) do not interfere with enzymatic assay of the HDL cholesterol.
Laboratorio di Chimica Clinica e di Ematologia, Centro Ospedaliero di Borgo Trento, Verona, Italy.
U
U
Lippi
M S
MS
Graziani
F
F
Manzato
M
M
Schinella
D001055:Apolipoproteins B / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood*; D014280:Triglycerides / Q000097:blood*
D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D014280:Triglycerides