Determination of nanogram/kilogram levels of polycyclic aromatic hydrocarbons in foods by HPLC with fluorescence detection.
Methodology was developed for the determination of eight polycyclic aromatic hydrocarbons (PAH) in five food categories including meat/fish, dried dairy products, cereals, leafy vegetables and vegetable/marine oils. The eight PAH were fluoranthene, benzo[a]anthracene, benzo[k]fluoranthene, benzo[b]fluoranthene, benzo[a]pyrene, 7,12-dimethylbenzo[a]anthracene, dibenzo[ah]anthracene and dibenzo[ai]pyrene. Samples were digested with alcoholic KOH followed by partitioning into solvents such as cyclohexane or isooctane. Lipids and other interferences were removed by solvent partitioning with dimethylformamide or dimethylsulfoxide/water. Additional cleanup involving column chromatography on silica gel, Florisil or Sephadex LH-20 was employed as required. Reversed-phase chromatography with gradient elution and fluorescence detection was employed for the determinations. Confirmation was carried out by GC-MS/SIM. Detection limits ranged from 2-90 ng/kg depending on the PAH and food analyzed. Results of a small survey indicated that the meat/fish category had the highest levels (low microgram/kg, on average) of the foods studied.
Lawrence
J F
JF
Das
B S
BS
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003611:Dairy Products / Q000032:analysis; D002523:Edible Grain / Q000032:analysis; D005399:Fishes; D005504:Food Analysis; D008460:Meat / Q000032:analysis; D008832:Microchemistry; D009821:Oils / Q000032:analysis; D011083:Polycyclic Compounds / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014675:Vegetables / Q000032:analysis
D009821:Oils; D011083:Polycyclic Compounds