Detection by ELISA of immunoglobulin G subclass-specific antibody responses in rotavirus infections in children.
IgG subclass-specific antibody responses to a human subgroup 2 rotavirus were studied in 26 children by ELISA by use of monoclonal antibodies specific to the four human IgG subclasses. One hundred twenty-nine serum samples were obtained before, during, and after an episode of rotavirus-induced diarrhoea in these patients. When these sera were investigated, an increase in IgG1 and IgG3 subclass-specific antibodies was detected in all 26 patients. IgG3 antibodies reached a peak concentration 1 week after rotavirus was detected in faecal samples and then progressively declined over the following months, whereas the peak concentration of IgG1 subclass antibodies was found 2 months later and seemed to persist thereafter. IgG2 rotavirus-specific subclass antibodies were never found and IgG4 subclass antibodies were detected only in sera from seven of the 26 patients.
P C
PC
Grauballe
A
A
Hornsleth
K
K
Hjelt
P A
PA
Krasilnikoff
D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000145:classification* / Q000276:immunology; D007223:Infant; D008297:Male; D012400:Rotavirus Infections / Q000097:blood / Q000276:immunology*; D013997:Time Factors
D007074:Immunoglobulin G