Detection of antigen immunologically related to basic pancreatic inhibitor (Kunitz) in porcine blood plasma by immunoaffinity chromatography.
Rabbit antiserum against trypsin-kallikrein inhibitor (TKI) was prepared. Purified immunoglobulin G (IgG) fraction was bound to Sepharose 4B. An antigen immunologically related to TKI was obtained from porcine blood plasma by adsorbing it onto the immunosorbent column. Its immunoreactivity with TKI antibodies was confirmed by immunoelectrophoresis. The antigen was an inhibitor of trypsin and acrosin.
Veselský
L
L
Jonáková
V
V
Cechová
D
D
D000176:Acrosin / Q000037:antagonists & inhibitors; D000818:Animals; D000941:Antigens / Q000032:analysis*; D002417:Cattle; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D007120:Immunochemistry; D007202:Indicators and Reagents; D011817:Rabbits; D013552:Swine; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000276:immunology* / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000276:immunology*
D000941:Antigens; D007202:Indicators and Reagents; D014361:Trypsin Inhibitors; D014360:Trypsin Inhibitor, Kunitz Soybean; D000176:Acrosin