The influence of prolonged di(2-ethylhexyl)phthalate treatment on the dolichol and dolichyl-P content of rat liver.
Dolichols and glycosyl transferase activities were studied in rat liver fractions after treatment with the plasticizer di(2-ethylhexyl)phthalate, an inducer of peroxisomes and mitochondria. After a few weeks of treatment with 2% plasticizer in the diet, the amount of dolichol is more than doubled in the lysosomes but not in the microsomes while dolichyl-P decreased by 50% in the microsomes but not in the lysosomes. The isoprenoid pattern for dolichol and dolichyl-P, respectively, is modified to longer polyprenols in the two fractions as seen in the percent distribution of the individual isoprenes. Dolichyl-P and protein glycosylation by N-acetylglucosamine and mannose decreased considerably. Incubation with mixtures containing exogenous dolichyl-P did not increase protein glycosylation. Phthalate ester treatment for 2 years increased dolichol content above the control values even when the dose was decreased a hundred times, to 0.02%. The results demonstrate a compartmentalization of dolichol and dolichyl-P distribution, and the induction studies suggest that hepatocytes possess separate regulating mechanisms for these two compounds.
Edlund
C
C
Ganning
A E
AE
Dallner
G
G
D000818:Animals; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004224:Diterpenes / Q000378:metabolism*; D004288:Dolichol Phosphates / Q000378:metabolism*; D004286:Dolichols / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D010795:Phthalic Acids / Q000494:pharmacology*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D004224:Diterpenes; D004288:Dolichol Phosphates; D004286:Dolichols; D010795:Phthalic Acids; D011106:Polyisoprenyl Phosphates; C028306:dolichol monophosphate; D004051:Diethylhexyl Phthalate; D006602:Hexosyltransferases