Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol.
A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 +/- 0.66 ng/ml (mean +/- S.D.).
Huber-Smith
M J
MJ
Nesse
R
R
Mazhar
M
M
McCann
D S
DS
D001800:Blood Specimen Collection; D002394:Catechol O-Methyltransferase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006018:Glycols / Q000097:blood*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000097:blood*; D011187:Posture; D012016:Reference Values; D013997:Time Factors
D006018:Glycols; D008734:Methoxyhydroxyphenylglycol; D002394:Catechol O-Methyltransferase