Amino acid sequence analysis of the neuronal type II calmodulin-dependent protein kinase by tandem mass spectrometry.
The primary structure of the neuronal Type II calmodulin-dependent protein kinase has been examined by protein sequence analysis and compared to cDNA-derived sequence. Tandem mass spectroscopic analysis was used for the sequence determination. Comparison with published cDNA sequence data for the alpha subunit revealed that the difference between the alpha- and beta-subunits lay in two insertions into the sequence for the alpha-subunit and a short alpha-specific sequence. The N-terminal amino acid of the alpha subunit which is blocked to Edman degradation has been tentatively identified as N-acetyl-alanine.
LeVine
H
H
Glaxo Research Laboratories, Research Triangle Park, NC 27709.
Hunt
D F
DF
Zhu
N Z
NZ
Shabanowitz
J
J
D000595:Amino Acid Sequence; D046911:Macromolecular Substances; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009474:Neurons / Q000201:enzymology; D010446:Peptide Fragments; D011487:Protein Conformation; D011494:Protein Kinases
D046911:Macromolecular Substances; D010446:Peptide Fragments; D011494:Protein Kinases