Dietary fish oils reduce plasma levels of platelet activating factor precursor (lyso-PAF) in rats.
The object of this study was to develop an assay for platelet activating factor (PAF) in rat plasma, and to utilise this to determine the effects of dietary fish oil on PAF in normotensive and spontaneously hypertensive rats. Measurement of platelet activating factor in blood plasma has proved difficult because of its rapid hydrolysis in vivo to lyso PAF. We describe here a method based on the prior acetylation of lyso PAF extracted from plasma to PAF before bioassay using 14C-serotonin labelled platelets. The active material found in acetylated plasma extracts was characterized as PAF by its chromatographic mobility, the action of phospholipases A2, C and D and by cross-desensitization studies with rabbit platelets. Rats fed dietary fish oil ('max EPA') had significantly decreased plasma lyso-PAF levels compared to control animals fed hydrogenated coconut oil (HCO). Serum thromboxane B2 (TXB2) levels were also significantly lower in animals fed the 'max EPA' diet. Spontaneously hypertensive rats (SHR) had significantly lower plasma lyso-PAF levels than their normotensive Wistar Kyoto (WKY) controls maintained on the same diets. It is proposed that dietary alterations in PAF synthesis may influence platelet behaviour in addition to the well described effects of dietary fish oil on the proaggregatory prostanoid TXA2. Rat strain differences in lyso-PAF synthesis occur, but are unlikely to be related to the maintenance of hypertension in SHR.
K D
KD
Croft
M J
MJ
Sturm
J P
JP
Codde
R
R
Vandongen
L J
LJ
Beilin
D000818:Animals; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000097:blood; D005395:Fish Oils / Q000494:pharmacology*; D006973:Hypertension / Q000097:blood; D008297:Male; D010740:Phospholipases / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000097:blood; D011817:Rabbits; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013929:Thromboxane B2 / Q000096:biosynthesis
D005227:Fatty Acids; D005395:Fish Oils; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D013929:Thromboxane B2; D010740:Phospholipases