Localization of specific mRNA sequences in Xenopus laevis embryos by in situ hybridization.
In situ hybridization of cloned cDNA probes to frozen sections of Xenopus laevis stage-42 tadpoles has been used to determine the tissue localization of several mRNAs. Nine out of sixteen probes tested hybridized to most or all tadpole tissues; seven probes exhibited tissue-specific hybridization. The non-tissue-specific sequences hybridized to RNA species that are also present in maternal RNA while the tissue-specific sequences hybridized to embryonic RNA species induced after gastrulation and undetectable in maternal RNA. Tissue-specific hybridization was observed with muscle (five clones), epidermis (one clone), and the nervous system (one clone). All muscle-specific sequences hybridized to somites and lateral plate muscles, but they differed in their hybridization to heart muscle.
E
E
Dworkin-Rastl
D B
DB
Kelley
M B
MB
Dworkin
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004625:Embryo, Nonmammalian / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger; D014982:Xenopus laevis
D012333:RNA, Messenger; D004247:DNA