Surface affinity chromatographic separation of blood cells. V. Retention behaviour of human peripheral blood cells on poly(propylene glycol) bonded agarose columns and its relationship to surface hydrophobicities of the gel beads and the cells.
Glycidyl ethers of poly(propylene glycol) (PPG) 200, 400 or 950 were oxirane-coupled with several kinds of agarose bead as the bonded phase of packing materials. An eluent was composed of 0.09 M phosphate-buffered 2% (w/w) dextran T40 solution at pH of respective isoelectric points of human blood cells. A linear relationship was found between the retention volumes of platelets, granulocytes and lymphocytes on the PPG-agarose columns and a measure of surface hydrophobicities of the cells, delta log K values, which were determined by using hydrophobic affinity partition. Furthermore, the retention volumes of granulocytes and lymphocytes increased according to the increase of delta log K of PPG-agarose beads. The retention of these two cells must be due to the hydrophobic interaction with the bonded PPG phase.
U
U
Matsumoto
Y
Y
Shibusawa
D000328:Adult; D001773:Blood Cells / Q000166:cytology*; D001792:Blood Platelets / Q000166:cytology; D002469:Cell Separation / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004912:Erythrocytes / Q000166:cytology; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D011108:Polymers; D011409:Propylene Glycols; D012685:Sepharose
D011108:Polymers; D011409:Propylene Glycols; C012504:polypropylene glycol; D012685:Sepharose