Carboxyfluorescein. A dye for evaluating the corneal endothelial barrier function in vivo.
The relationship of the cornea-aqueous distribution ratio (r ca) and concentration in vitro was established for fluorescein and carboxyfluorescein. The value of rca for fluorescein was found to fall from 3.20 +/- 0.25 (mean +/- S.D., n V 6) to 1.78 as the concentration of the free fluorescein in the bathing medium rose from 5.8 X 10(-8) to 5.9 X 10(-5) g ml-1. For carboxyfluorescein, it remained unchanged over the same concentration range, and the average for total determinations was 1.29 +/- 0.16 (n = 20). The value of rca for carboxyfluorescein determined in vivo was 1.62 +/- 0.23 (mean +/- S.D., n = 6) and the corneal endothelial permeability to carboxyfluorescein in normal rabbits was 3.31 +/- 0.66 X 10(-4) cm min-1 (n = 11), which was 35% lower than that for fluorescein. Because of its lower endothelial permeability and a value of rca which is unchanged over a wide range of concentration, carboxyfluorescein may be better suited for the in vivo evaluation of the barrier function of the corneal endothelium than fluorescein.
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D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D002463:Cell Membrane Permeability; D003315:Cornea / Q000378:metabolism*; D004058:Diffusion; D004727:Endothelium / Q000378:metabolism; D019793:Fluorescein; D005452:Fluoresceins / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors
D005452:Fluoresceins; C024098:6-carboxyfluorescein; D019793:Fluorescein