[Determination of trimethoquinol in plasma by electrochemical detection after HPLC separation].
A high pressure liquid chromatographic method with internal analogue standardization for the determination of trimethoquinol (1-1-(3,4,5-trimethoxybenzyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoq uinoline hydrochloride) in plasma is described. The method comprises extraction from plasma at pH 9 with ethyl acetate, back-extraction into phosphoric acid and quantification after HPLC separation using a reverse phase by means of electrochemical (reductive) detection after electrochemical preoxidation. When using 5 ml plasma the lower limit of detection is 40 pg/ml. Under routine conditions the limit of determination is determined to be 0.1 ng/ml. The variation coefficients drop from about 7% in the low range to about 4% at 5 ng/ml. The determination of trimethoquinol is not impaired by its monomethylated metabolites.
Brode
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E
Binder
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Breckwoldt
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D000085:Acetates / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007546:Isoquinolines / Q000097:blood*; D010756:Phosphoric Acids / Q000494:pharmacology; D051381:Rats; D012015:Reference Standards; D014213:Tretoquinol / Q000097:blood* / Q000378:metabolism
D000085:Acetates; D007546:Isoquinolines; D010756:Phosphoric Acids; C007650:ethyl acetate; C030242:phosphoric acid; D014213:Tretoquinol
Bestimmung von Trimethoquinol in Plasma mittels elektrochemischer Detektion nach HPLC-Trennung.