[Specific binding of (+)-3H-SKF 10047 by mouse splenocytes].
Stereoselective, saturable, reversible and temperature-dependent binding of ligand of sigma opioid receptors (+)-3H-SKF 10047 to mouse splenocytes has been demonstrated. The estimated Kd value of this interaction (20-100 nM) was similar to that of (+)-3H-SKF 10047-sigma receptor complex. The binding capacity was 100 molecules per cell. A comparatively low affinity, non-stereoselective and temperature-independent binding of (+)-3H-SKF 10047 to mouse splenocytes has also been found.
Iarygin
K N
KN
Sukhikh
G T
GT
Vinogradov
V A
VA
Kryzhanovskiĭ
G N
GN
D000818:Animals; D066298:In Vitro Techniques; D007536:Isomerism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010620:Phenazocine / Q000031:analogs & derivatives* / Q000378:metabolism; D013045:Species Specificity; D013154:Spleen / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; C012134:SK&F 10047; D010620:Phenazocine
Spetsificheskoe sviazyvanie (+)-3H-SKF 10047 splenotsitami myshi.