Immunohistochemical evidence for degeneration of cholinergic neurons in the forebrain of the rat following injection of AF64A-picrylsulfonate into the dorsal hippocampus.
The cholinergic neurotoxin, AF64A-picrylsulfonate, was unilaterally infused into the dorsal hippocampus of Wistar rats (2 nmol/2 microliters/4 min; A 6.2, Ls 1.5, H 6.5, Paxinos and Watson). After 19 days the for immunohistochemical staining of choline acetyltransferase (ChAT). Morphometry and counting of ChAT-immunoreactive profiles revealed shrinkage and disappearance of cholinergic neurons in the medial septum and diagonal band of Broca at the lesioned brain side. These data indicate a retrograde degeneration of cholinergic neurons following injection of AF64A-picrylsulfonate into the dorsal hippocampus of the rat.
J A
JA
Tonnaer
A J
AJ
Lammers
J H
JH
Wieringa
H W
HW
Steinbusch
D000544:Alzheimer Disease / Q000473:pathology; D000818:Animals; D001388:Aziridines / Q000494:pharmacology*; D001389:Azirines / Q000494:pharmacology*; D002794:Choline / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D002799:Cholinergic Fibers; D004027:Diencephalon / Q000473:pathology*; D004195:Disease Models, Animal; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism; D013687:Telencephalon / Q000473:pathology*; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology
D001388:Aziridines; D001389:Azirines; D012701:Serotonin; D014302:Trinitrobenzenesulfonic Acid; C044894:ethylcholine aziridinium; D002795:Choline O-Acetyltransferase; D002794:Choline