Selective determination of lactate dehydrogenase isoenzyme 1 in serum after inhibition by 1,6-hexanediol.
A rapid selective method for measuring the activity of lactate dehydrogenase isoenzyme LD-1 in serum by using 1,6-hexanediol as an inhibitor of the M-subunit was developed. Hexanediol was added to serum at a final concentration of 0.7 mol/l. After incubation at 30 degrees C for 15 min, the activity was measured with an automatic analyser. The inter-assay coefficient of variation was 6.9% for the lactate dehydrogenase isoenzyme LD-1 measurement. The results obtained from the sera of 100 patients analysed by the proposed selective method and by the conventional electrophoretic method, respectively, showed an excellent correlation. This selective method was used to determine the lactate dehydrogenase isoenzyme LD-1 activity of sera from patients with acute myocardial infarction, and the results were correlated well with those obtained by the immunological, Roch Isomune method. Addition of 1,6-hexanediol did not affect the measurement of activities of other enzymes such as alkaline phosphatase, gamma-glutamyltransferase, aspartate aminotransferase and alanine aminotransferase.
Division of Clinical Laboratory Sciences, School of Allied Medical Professions, Kanazawa University, Japan.
K
K
Tanishima
E
E
Ohta
J
J
Mikami
S
S
Sumita
D004586:Electrophoresis; D006018:Glycols / Q000494:pharmacology*; D006801:Humans; D007120:Immunochemistry; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors / Q000097:blood*; D009203:Myocardial Infarction / Q000201:enzymology
D006018:Glycols; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; C027765:hexamethylene glycol