Lymphocyte proliferation and cytotoxic assays using flat-bed scintillation counting.
Lymphocyte 51Cr release and [3H]thymidine uptake assays were evaluated with respect to measurement of sample radioactivity using the flat-bed scintillation counter. 51Cr lysates were spotted onto a glass fibre filter sheet while [3H]thymidine-labelled cells were filtered onto a similar sheet using a cell harvester. The 96 samples were rapidly processed for counting, without removal of individual sample areas. Either form of preparation showed good linearity of count rate with the quantity of material on the filter. Reproducibility was good; the coefficient of variation for 96 samples being within 5%. The low background and high efficiency of this counter results in increased assay sensitivity and allows considerable economies in materials to be made. A commercial version of the counter has six counting heads permitting a high rate of sample throughput.
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, U.K.
C G
CG
Potter
F
F
Gotch
G T
GT
Warner
J
J
Oestrup
D001610:Beta Particles; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic; D005720:Gamma Rays; D006801:Humans; D008213:Lymphocyte Activation; D012588:Scintillation Counting / Q000295:instrumentation*; D014316:Tritium
D002860:Chromium Radioisotopes; D014316:Tritium