Increased lymphocyte transport by lipid absorption in rat mesenteric lymphatics.
The effect of olive oil administration on lymphocyte transport through mesenteric lymphatics was examined to see the possible involvement of nutritional absorption in lymphocyte traffic from the intestinal mucosa. After the olive oil administration to rats, remarkable increase in lymphocyte flux was observed within 2 h in lymph samples collected from rats with lymphatic fistula. The use of a high-speed microscopic video system made it possible to analyze accurately the lymphocyte transport in rapid movement that could not be detected by any of the ordinary video systems. The direct observation of mesenteric collecting lymphatics by this system showed an increment of lymphocyte transport from the intestinal mucosa by lipid absorption in 2 h. The contraction frequency of intestinal collecting lymphatics was also enhanced by olive oil administration. The densitometric analysis on video image was applied to estimate the extent of lipid absorption. The combination of a high-speed video system and the densitometric analysis revealed that the increase in lymphocyte flux occurred before lipid absorption reached its maximum and also demonstrated that the lymphocyte transport returned to control levels under the maximal absorptive condition. The results suggest that the fat absorption could be an important factor influencing the lymphocyte transport in the lymphatic system of intestine.
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
S
S
Miura
E
E
Sekizuka
H
H
Nagata
C
C
Oshio
H
H
Minamitani
M
M
Suematsu
M
M
Suzuki
Y
Y
Hamada
K
K
Kobayashi
H
H
Asakura
D000042:Absorption; D000818:Animals; D001692:Biological Transport; D004042:Dietary Fats, Unsaturated; D007091:Image Processing, Computer-Assisted; D050356:Lipid Metabolism; D008196:Lymph / Q000378:metabolism; D008208:Lymphatic System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008643:Mesentery / Q000033:anatomy & histology*; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism; D014741:Video Recording
D004042:Dietary Fats, Unsaturated; D000069463:Olive Oil; D010938:Plant Oils; D011506:Proteins; D014280:Triglycerides