Muscarine binding sites in bovine adrenal medulla.
The presence of muscarinic binding sites in the bovine adrenal medulla was investigated using [3H]QNB and the bovine adrenal medulla. Scatchard analysis combined with computer analysis yielded data consistent with a two binding site configuration. KDs of 0.15 and 14 nM and Bmax s of 29 and 210 fmol/mg protein, respectively, were observed. Displacement of [3H]QNB by various cholinergic agents is, in order of decreasing potency: QNB, dexetimide, atropine, scopolamine, imipramine, desipramine, oxotremorine, pilocarpine, acetylcholine, methacholine and carbachol. These results demonstrate the presence of more than one muscarine binding site in the bovine adrenal gland.
B A
BA
Barron
L C
LC
Murrin
T D
TD
Hexum
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002838:Chromaffin System / Q000378:metabolism; D066298:In Vitro Techniques; D009116:Muscarine / Q000378:metabolism*; D010277:Parasympathomimetics / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate; D011869:Radioligand Assay; D011976:Receptors, Muscarinic / Q000378:metabolism*
D010277:Parasympathomimetics; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D009116:Muscarine