Bovine brain factor promotes neurite extension from chick embryo dorsal root ganglia in vitro.
A protein with neurite-stimulating activity was isolated from bovine brain tissue lysosomal fraction. The organotypic culture of dorsal root ganglia (DRG) of 10-11-day-old chick embryo was used to test neurite-stimulating activity. A cation protein (16,000 Da) has been purified using a combination of extraction of acid-soluble proteins, preparative electrophoresis, and affinity chromatography on heparin-sepharose. This protein is active at 2-4 ng/ml inducing extensive neurite outgrowth in this bioassay compared to the control one. Many properties distinguish this factor from other known neuronotrophic factors.
Goncharova
V
V
Leningrad State University, I.P. Pavlov Institute of Physiology, Academy of Sciences, U.S.S.R.
Romanjuk
A
A
Chalisova
N
N
Akoev
G
G
Chumasov
E
E
Sotnikov
O
O
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002478:Cells, Cultured; D002642:Chick Embryo; D003712:Dendrites / Q000187:drug effects / Q000502:physiology*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D013347:Subcellular Fractions / Q000032:analysis
D009414:Nerve Growth Factors