In vitro production of glycosaminoglycans by retinal microvessel cells and lens epithelium.
Analysis of bovine lens capsules and retinal microvessel basement membranes by cellulose acetate electrophoresis, together with treatment by specific enzymes or by nitrous acid, reveals that heparan sulfate is the only demonstrable glycosaminoglycan (GAG), consistent with the observations of most other investigators on basement membrane preparations from several different tissues. When bovine retinal microvessel pericytes or endothelial cells or bovine lens epithelial cells are grown in culture in the presence of [35S]-sulfate or [3H]-glucosamine, however, both cellulose acetate electrophoresis and gel filtration chromatography reveal that the cultured pericytes synthesize primarily chondroitin sulfate, whereas the lens epithelial cells and microvascular endothelial cells produce a mixture of GAGs consisting of approximately 60% heparan sulfate and 40% chondroitin sulfate. The chondroitin sulfate chains have an Mr of 70,000, but the Mr of the heparan sulfate chains is 10,000, based on gel filtration chromatography on Sepharose CL-6B. Hence, cell culture conditions may produce phenotypic modulation of the biosynthetic capacities of these cells for GAGs. However, the difference between the in vitro and in vivo findings may in part be explained if many of the chondroitin sulfate GAGs synthesized in vitro are cell associated and are not incorporated into the basement membrane.
A
A
Kennedy
R N
RN
Frank
M A
MA
Mancini
D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D002417:Cattle; D002845:Chromatography; D006025:Glycosaminoglycans / Q000032:analysis* / Q000302:isolation & purification; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000032:analysis*; D010857:Pigment Epithelium of Eye / Q000032:analysis*; D012171:Retinal Vessels / Q000032:analysis*
D006025:Glycosaminoglycans