Consequences of cadmium toxicity in rat hepatocytes: effects of cadmium on the glutathione-peroxidase system.
Cadmium (Cd; 10-100 microM) produced in isolated hepatocytes the formation of thiobarbituric acid-reactants (lipid peroxidation; LPO) and a decrease in SH groups in a time- and concentration-dependent manner. However, glutathione peroxidase (GSH-Px) was not affected indicating that LPO does not originate from GSH-Px injury. In contrast, in presence of Cd a time- and concentration-dependent decrease of glucose-6-phosphate dehydrogenase (G6PDH) and, most strongly, of glutathione reductase (GSSG-R), both members of the GSH-Px system, was observed. These effects could not be inhibited by (+)-cyanidanol-3, a compound known as inhibitor of LPO. Therefore, our data show that Cd-induced LPO is not a function of GSH-Px injury and inhibition of GSSG-R and G6PDH-activity is not a function of LPO. They also indicate that Cd-induced depletion of cellular SH groups are associated with toxic effects of Cd on GSSG-R and G6PDH different from LPO-induction.
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D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism; D008054:Lipid Peroxides / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D008054:Lipid Peroxides; D002104:Cadmium; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione