Microcomputer simulation of antigen-antibody binding at a fixed antigen/antibody ratio.
Computer simulation techniques were used to define the theoretical nature of the reaction of antigen with antibody at a constant degree of antigen excess. The simulation studies show that binding curves obtained under these conditions have unique features which are determined by antibody affinity, affinity heterogeneity and the concentrations of antigen and antibody. When analyzed by non-linear least squares regression these curves can be used to determine the affinity of antibody in homogeneous and heterogeneous systems.
D P
DP
Nelson
W R
WR
Griswold
D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D003201:Computers; D008838:Microcomputers; D008954:Models, Biological