Cadmium-binding proteins in the scallop Pecten maximus.
Scallops, Pecten maximus, accumulate cadmium naturally in the digestive gland to a level of approximately 100 ppm wet weight. Of this cadmium, 60% was soluble and was composed of three weight classes as judged by Sephadex G-100 chromatography. Of the soluble cadmium, 60% was in the 55,000 molecular weight range and 20% each in an excluded fraction and a 10,000 molecular weight fraction. The 55,000 molecular weight fraction, after further purification, showed a maximum cadmium concentration of 1.4% by weight. The cadmium was thiolate bound but not as strongly bound as in the case of metallothionein. The 10,000 molecular weight fraction was a metallothionein-like protein.
H C
HC
Stone
S B
SB
Wilson
J
J
Overnell
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002104:Cadmium / Q000032:analysis*; D002850:Chromatography, Gel; D004064:Digestive System / Q000032:analysis; D008668:Metallothionein / Q000032:analysis*; D008970:Molecular Weight; D008974:Mollusca / Q000032:analysis*
D000596:Amino Acids; C004203:cadmium-binding protein; D002104:Cadmium; D008668:Metallothionein