The elimination of mutagenic lesions induced by alkylating agents and UV in V79 Chinese hamster cells.
The mutagenicity of MNU, EMS, BMS and UV light was compared by analyzing the dose-response curve just before and after the replicative process of the HGPRT gene in synchronized V79 Chinese hamster cells. This system makes it possible to compare a 10-h period for repair of different mutagenic lesions with no time for repair. Additional time for repair in synchronized V79 cells resulted in a reduced response for MNU and UV, but not for EMS and BMS. This result suggests that an error-free repair process operates on mutagenic lesions in methylated DNA and on thymine dimers, but not on ethylated and butylated DNA. Based on these results, it is concluded that the repair capacity of V79 cells to remove mutagenic lesions is characterized as low for UV, moderate for MNU and not detectable for the mutagenic lesions induced by EMS and BMS.
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D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D000477:Alkylating Agents; D007041:Hypoxanthine Phosphoribosyltransferase