Enhancing effects of bovine serum albumin on cell injury in vitro induced with Fe-NTA.
Direct effects of ferric nitrilotriacetate (Fe-NTA) on normal rat liver epithelial cells (RL34) in serum-free culture were studied. More than 10 micrograms/ml iron of Fe-NTA was cytotoxic and the cytotoxicity was prevented by adding apotransferrin into culture medium. Also, bovine serum albumin (BSA), which is known to bind Fe-NTA, was found to promote the cytotoxicity, while fatty acids-free BSA (F-BSA) prevented it. This result indicates that fatty acids rather than albumin promote Fe-NTA cytotoxicity.
Division of Cell Biology, Shigei Medical Research Institute, Okayama, Japan.
M
M
Yamada
T
T
Okigaki
M
M
Awai
D000818:Animals; D001059:Apoproteins / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D004848:Epithelium / Q000187:drug effects; D005227:Fatty Acids / Q000494:pharmacology; D005290:Ferric Compounds / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D014168:Transferrin / Q000494:pharmacology
D001059:Apoproteins; D005227:Fatty Acids; D005290:Ferric Compounds; D014168:Transferrin; C028284:apotransferrin; D012710:Serum Albumin, Bovine; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate