Liquid-chromatographic determination of 4-hydroxyproline in urine.
In this method for 4-hydroxyproline in urine, hydroxyproline is derivatized with 4-chloro-7-nitrobenzofurazan, with subsequent estimation by reversed-phase "high-performance" liquid chromatography. The ranges for excretion of free and total hydroxyproline while the subjects were ingesting unrestricted diets were 2-29 and 122-374 mumol/24 h (n = 21), respectively, with no significant sex-related difference. A comparison with results by colorimetry indicated no significant differences: mean (n = 18) concentrations (mumol/L) of hydroxyproline in urine were 180 (SD 149) by the present method, 163 (SD 166) by colorimetry. For protein hydrolysate the respective values were 5.9 (SD 2.7) and 6.7 (SD 2.9).
H
H
Hughes
L
L
Hagen
R A
RA
Sutton
D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D008297:Male; D012016:Reference Values
D006909:Hydroxyproline