P-45011 beta-dependent conversion of cortisol to cortisone, and 19-hydroxyandrostenedione to 19-oxoandrostenedione.
Purified bovine adrenal P-45011 beta has been shown to catalyze conversions of cortisol to cortisone (11-oxidase activity), and 19-hydroxyandrostenedione to 19-oxoandrostenedione (19-oxidase activity), in the reconstituted system consisting of NADPH, NADPH:adrenodoxin reductase, and adrenodoxin. The turnover numbers (mol of product formed/min/mol of P-450) were 1.2 for the 11-oxidase activity and 1.4 for the 19-oxidase activity. No reactions took place when any one of the electron-donating components were omitted either in the presence or in the absence of added NADP+. Likewise, rabbit antibody prepared against P-45011 beta immunoprecipitated the 11-oxidase activity with concomitant loss of deoxycorticosterone 11 beta-hydroxylase activity.
K
K
Suhara
K
K
Takeda
M
M
Katagiri
D000311:Adrenal Glands / Q000201:enzymology; D000325:Adrenodoxin / Q000378:metabolism; D000735:Androstenedione / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003348:Cortisone / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006854:Hydrocortisone / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D011817:Rabbits
D007527:Isoenzymes; D000325:Adrenodoxin; D000735:Androstenedione; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; C014142:19-oxo-delta(4) androstene-3,17-dione; C011482:19-hydroxy-4-androstene-3,17-dione; D005287:Ferredoxin-NADP Reductase; D003348:Cortisone; D006854:Hydrocortisone