Isolation and some properties of exohemagglutinin from the culture medium of Bacteroides gingivalis 381.
Exohemagglutinin was found in the culture medium of Bacteroides gingivalis 381. Exohemagglutinin was purified 3,150-fold from culture fluid by ultracentrifugation followed by gel filtration on Sepharose CL-4B and by affinity chromatography on arginine-agarose. Examination of the final preparation of exohemagglutinin by biochemical analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the isolated exohemagglutinin contained three major proteins but not a detectable lipopolysaccharide. Hemagglutination inhibition experiments showed that the activity of exohemagglutinin was inhibited by L-arginine and the arginine-containing peptides, although the activity was unaffected by the sugars tested. Some protein and glycoproteins that were examined also exhibited the inhibitory activity. When the bovine submaxillary mucin was chemically modified by beta-elimination and bovine serum albumin was modified by guanidination, the inhibitory effects on hemagglutination were significantly enhanced. These results suggest that the hemagglutination of the isolated exohemagglutinin may be involved in arginine residues as components of ligand-binding sites on erythrocytes.
Inoshita
E
E
Amano
A
A
Hanioka
T
T
Tamagawa
H
H
Shizukuishi
S
S
Tsunemitsu
A
A
D001426:Bacterial Proteins / Q000302:isolation & purification; D001439:Bacteroides / Q000032:analysis*; D003470:Culture Media; D006023:Glycoproteins / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests; D006388:Hemagglutinins / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight; D009842:Oligopeptides / Q000494:pharmacology; D009994:Osmolar Concentration; D011506:Proteins / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D006023:Glycoproteins; D006388:Hemagglutinins; D008670:Metals; D009842:Oligopeptides; D011506:Proteins