Method for the typing of Clostridium difficile based on polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins.
A typing method for Clostridium difficile based on the incorporation of [35S]methionine into cellular proteins, their separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their visualization by autoradiography is described. On analysis of the radiolabeled-protein profiles, nine distinct groups were observed (A to E and W to Z). The method, which is simple, reproducible, and readily expandable, has been applied in epidemiological studies to demonstrate cross-infection and hospital acquisition of C. difficile.
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S
Tabaqchali
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S
O'Farrell
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D
Holland
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R
Silman
D000328:Adult; D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis*; D003013:Clostridium / Q000032:analysis / Q000145:classification* / Q000302:isolation & purification; D003967:Diarrhea / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D008715:Methionine; D013462:Sulfur Radioisotopes
D001426:Bacterial Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine