New methodology for the synthesis of 2'(3')-O-aminoacyl oligoribonucleotides related to the 3'-terminus of aa-tRNA.
A new synthetic approach to the title compounds is reported. It is based on the phosphotriester methodology and uses a unique combination of protecting groups (Fmoc and Ph for the aglycons; DMT, CPTr and Mthp for the carbohydrate hydroxy functions; 2-CIPh for the phosphodiester bonds; BPOC for amino acid). This enables selective deprotection of the blocked oligonucleotide intermediates in two steps (oximate and acid treatments) to yield the 2'(3')-O-aminoacyl oligoribonucleotides suitable for biochemical investigations.
Hagen
M D
MD
Department of Chemistry, Michigan Cancer Foundation, Detroit 48201.
Scalfi-Happ
C
C
Happ
E
E
Chládek
S
S
D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D012346:RNA, Transfer, Amino Acyl / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012346:RNA, Transfer, Amino Acyl