Lipid changes of astrocytes from mouse cerebellum cultured in lipid-free chemically defined medium and in serum-supplemented medium.
Astrocytes of mouse cerebellum were grown in chemically defined medium for 14 days, subcultivated and grown in serum-containing medium for up to 14 days. After 14 days in defined medium the cells showed fatty degeneration from which they recovered histologically and chemically after one week in serum-supplemented medium. At this point 99% of the cells were glial fibrillary acidic protein-positive and synthesized phosphatidylinositol, phosphatidylserine and bis(monoacylglycero)phosphate besides other phospholipids.
Institut für physiologische Chemie, Universität zu Köln, F.R.G.
D
D
Krause
H
H
Debuch
D000818:Animals; D001253:Astrocytes / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D001798:Blood Proteins; D002478:Cells, Cultured; D002531:Cerebellum / Q000032:analysis / Q000378:metabolism*; D003470:Culture Media; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D051379:Mice; D010743:Phospholipids / Q000378:metabolism
D001798:Blood Proteins; D003470:Culture Media; D005904:Glial Fibrillary Acidic Protein; D008055:Lipids; D010743:Phospholipids