Inhibition of acetoacetyl-CoA synthetase from rat liver by fatty acyl-CoAs.
The activity of acetoacetyl-CoA synthetase from rat liver was found to be negatively regulated by coenzyme A, fatty acyl-CoAs and acetoacetyl-CoA in vitro. With increasing concentrations of coenzyme A (substrate inhibition occurring at concentrations higher than 50 microM) the pH optimum shifted toward the acidic side (7.5-8.5 with 5 microM coenzyme A and 6.5-7.0 with 500 microM coenzyme A), in parallel with progressively decreasing enzyme activity. Fatty acyl-CoAs of various chain lengths dose-dependently inhibited acetoacetyl-CoA synthetase from rat liver, but much less effectively a similar enzyme from a bacterium, Zoogloea ramigera I-16-M. Palmitoyl-CoA, the most potent inhibitor of the rat liver enzyme, with an apparent Ki value of 9.8 microM, apparently inhibited the enzyme below its critical micellar concentration, not due to its detergent action. Acetoacetyl-CoA showed product inhibition with a Ki value of 15 microM. These results suggest a possible physiological regulation mechanism for this enzyme with respect to fatty acid biosynthesis.
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
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M
Ito
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T
Fukui
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T
Saito
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Tomita
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000818:Animals; D003066:Coenzyme A Ligases / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D051381:Rats
D000214:Acyl Coenzyme A; D010171:Palmitoyl Coenzyme A; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase