Nucleophilic catalysis in the oligonucleotide synthesis.
Rapid procedure for the synthesis of oligonucleotides by the phosphotriester method has been developed. It is based on the use of O-nucleophilic intramolecular catalysis. The application of this method to automated solid-phase oligonucleotide synthesis allows to perform one elongation cycle for 6-7 min.
Shemyakin Institute of Bio-organic Chemistry, USSR Academy of Sciences, Moscow.
V A
VA
Efimov
O G
OG
Chakhmakhcheva
N N
NN
Polushin
I Y
IY
Dubey
D002384:Catalysis; D007202:Indicators and Reagents; D008722:Methods; D009841:Oligonucleotides / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009841:Oligonucleotides