Effects of sex steroid hormones on differentiation of adipose precursor cells in primary culture.
The effects of estradiol-17 beta and progesterone on multiplication, differentiation and lipid filling of adipose precursor cells were examined in primary cell cultures of cells prepared from adipose tissue of both male and ovariectomized female rats. Progesterone down to a concentration of 10(-7) mol/liter, alone or in the presence of estradiol-17 beta stimulated the development of glycerophosphate dehydrogenase and lipoprotein lipase activity. Estradiol-17 beta alone had no effects. These effects were essentially parallel to increases in the rate of lipid filling of the cells. Furthermore, the formation of cells with a lipid vacuole greater than 20 micron was markedly stimulated, suggesting that new fat cells were formed by the stimulation of differentiation of the adipose precursor cells. No effects of the sex steroid hormones were seen on the rate of multiplication. These results suggest a role of sex steroid hormones in the regulation of triglyceride storage capacity in adipose tissue by facilitating the differentiation of precursor cells to form new adipocytes.
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Department of Medicine I, Sahlgren's Hospital, Göteborg, Sweden.
Björntorp
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D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D014280:Triglycerides; D011374:Progesterone; D004958:Estradiol; D004247:DNA; D005993:Glycerolphosphate Dehydrogenase; D008071:Lipoprotein Lipase