Flurothyl seizure thresholds in mice treated neonatally with a single injection of monosodium glutamate (MSG): evaluation of experimental parameters in flurothyl seizure testing.
Monosodium glutamate (MSG) administration to neonatal rodents produces convulsions and results in numerous biochemical and behavioral deficits. These studies were undertaken to determine if neonatal administration of MSG produced permanent alterations in seizure susceptibility, since previous investigations were inconclusive. A flurothyl ether seizure screening technique was used to evaluate seizure susceptibility in adult mice that received neonatal injections of MSG (4 mg/g and 1 mg/g). MSG treatment resulted in significant reductions in whole brain weight but did not alter seizure threshold. A naloxone (5 mg/kg) challenge was also ineffective in altering the seizure thresholds of either control of MSG-treated mice. Flurothyl ether produced hypothermia which was correlated with the duration of flurothyl exposure; however, the relationship of hypothermia to seizure induction was unclear. Flurothyl seizure testing proved to be a rapid and reliable technique with which to evaluate seizure susceptibility.
Dawson
R
R
Department of Pharmacodynamics, University of Florida, College of Pharmacy, J. Hillis Miller Health Center, Gainesville 32610.
Bierkamper
G
G
D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D001921:Brain / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005481:Flurothyl / Q000009:adverse effects*; D005971:Glutamates / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009270:Naloxone / Q000009:adverse effects; D009929:Organ Size / Q000187:drug effects; D012640:Seizures / Q000139:chemically induced*; D012970:Sodium Glutamate / Q000009:adverse effects*; D013696:Temperature
D005971:Glutamates; D009270:Naloxone; D005481:Flurothyl; D012970:Sodium Glutamate