Concatemeric forms of intracellular Tupaia herpesvirus DNA.
Kinetics of Tupaia herpesvirus strain 2 (THV-2) DNA synthesis was measured by labeling with radioactive thymidine and determining the amount of newly synthesized DNA. Viral DNA synthesis reached a maximum between 24 to 36 hr postinfection, preceded by a transient stimulation of host cell DNA synthesis. Blot hybridizations of labeled recombinant plasmids harboring inserts of both genomic termini of THV-2 and of individual viral terminal DNA fragments to DNA of virus-infected cells and combined restriction enzyme analysis of labeled intracellular viral DNA revealed the presence of linear concatemeric and/or circular viral DNA molecules.
H G
HG
Koch
R M
RM
Flügel
G
G
Darai
D000818:Animals; D002478:Cells, Cultured; D004261:DNA Replication; D004270:DNA, Circular / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis*; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D014417:Tupaia; D014779:Virus Replication
D004270:DNA, Circular; D004279:DNA, Viral