Crystallization of a DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G).
Crystals of the DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G) have been grown by the vapor-diffusion technique with 2-methyl-2,4-pentanediol as precipitant. They are monoclinic space group C2, with a = 79.6 A, b = 43.1 A, c = 24.9 A and beta = 98.7 degrees. Previous nuclear magnetic resonance studies predicted that this tridecamer forms a duplex similar to the B DNA dodecamer, d(C-G-C-G-A-A-T-T-C-G-C-G), except for an extra adenosine residue that is stacked within the helix but remains unpaired: (formula; see text) Preliminary X-ray diffraction studies confirmed that the tridecamer is in the B DNA conformation, consistent with the nuclear magnetic resonance results.
Saper
M A
MA
Eldar
H
H
Mizuuchi
K
K
Nickol
J
J
Appella
E
E
Sussman
J L
JL
D003461:Crystallography; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides
D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides