Content of thiamin phosphate esters in mammalian tissues--an extremely high concentration of thiamin triphosphate in pig skeletal muscle.
In relation to a high activity of thiamin diphosphate (TDP) kinase (Koyama, S. et al. (1985) Biochem. Int. 11, 371-380) in the skeletal muscle of pigs and guinea pigs, the content of thiamin phosphate esters in tissues of these animals has been determined by the method of high-performance liquid chromatography. An extremely high concentration of thiamin triphosphate (TTP), 69.2% of the total thiamin (26.1 nmol/g wet weight), was detected in adult pig skeletal muscles. One extreme case contained TTP as 88.7% of the total thiamin (19.6 nmol/g wet weight). TTP in pig skeletal muscle was found solely in cytosol fraction. This is the first report showing an unusually high level of TTP in mammals and may give a clue as to the physiological functions of TTP.
Y
Y
Egi
S
S
Koyama
H
H
Shikata
K
K
Yamada
T
T
Kawasaki
D000375:Aging; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D009132:Muscles / Q000032:analysis*; D009928:Organ Specificity; D051381:Rats; D013045:Species Specificity; D013552:Swine; D013831:Thiamine / Q000031:analogs & derivatives* / Q000032:analysis*; D013833:Thiamine Monophosphate / Q000032:analysis*; D013835:Thiamine Pyrophosphate / Q000032:analysis*; D013838:Thiamine Triphosphate / Q000032:analysis*
D013838:Thiamine Triphosphate; D013833:Thiamine Monophosphate; D013835:Thiamine Pyrophosphate; D013831:Thiamine