[Strengthening of the DNA-protein complex during the stationary aging of cultured cells].
Accumulation of DNA-protein crosslinks during "stationary phase ageing" (i.e. with the increase of non-proliferating period) of cultured transformed Chinese hamster cells and normal embryonic diploid human fibroblasts was determined by the improved method of column chromatography using hydroxylapatite of 14C-valine, 14C-leucine and 3H-thymidine-labelled cell homogenates. Such an accumulation, however, was not observed during "stationary phase ageing" of xeroderma pigmentosum human fibroblasts. Possible mechanisms of the above phenomenon are discussed.
Khokhlov
A N
AN
Chirkova
E Iu
EIu
Gorin
A I
AI
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors; D014983:Xeroderma Pigmentosum / Q000378:metabolism
D011506:Proteins; D004247:DNA
Uprochnenie DNK-belkovogo kompleksa v protsesse "statsionarnogo stareniia" kul'tiviruemykh kletok.