[Detection of diphtheria toxin and its degradation products by using immunoenzyme analysis].
An enzyme immunoassay system for the detection of diphtheria toxin and the products of its degradation has been developed on the basis of the enzyme-linked immunosorbent assay. To sensitize the assay plates, bivalent F(ab)2-fragments of purified antidiphtheria antibodies at a concentration of 10.0 micrograms/ml, obtained from the blood serum of hyperimmunized rabbits, have been used. Specific conjugates have been prepared with the use of F(ab)2-fragments of purified antidiphtheria antibodies obtained from the blood serum of hyperimmunized horses. The optimum time and temperature conditions of the assay have been established. The new enzyme immunoassay system permits the detection of diphtheria toxin and the products of its degradation in biological substrates at a concentration of 10.0-5.0 ng/ml.
S V
SV
Shabalina
L I
LI
Raĭkher
I I
II
Raĭkher
L Iu
LIu
Smurova
E Kh
EKh
Khazina
D000818:Animals; D004167:Diphtheria Toxin / Q000032:analysis*; D004168:Diphtheria Toxoid / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005069:Evaluation Studies as Topic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007202:Indicators and Reagents; D011817:Rabbits; D013696:Temperature; D013997:Time Factors
D004167:Diphtheria Toxin; D004168:Diphtheria Toxoid; D007140:Immunoglobulin Fab Fragments; D007202:Indicators and Reagents
Vyiavlenie difteriĭnogo toksina i produktov ego degradatsii s pomoshch'iu immunofermentnogo analiza.