Quantitative analysis of 1-(2-pyrimidinyl)piperazine in plasma by capillary gas chromatography-mass spectrometry.
A sensitive method for detecting and quantifying 1-(2-pyrimidinyl)piperazine, an important metabolite of buspirone, in human plasma was developed and validated. The range of the method is 0.2-15 ng/ml. The analyte was removed from buffered, sodium chloride-saturated plasma with benzene, extracted into aqueous acid, washed with diethyl ether and reextracted into benzene. The processed extract was derivatized with pentafluorobenzoyl chloride. Instrumental analysis involved on-column injection into a fused-silica capillary gas chromatography column and detection by selected-ion monitoring mass spectrometry.
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Kerns
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Bullen
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Gammans
D002065:Buspirone / Q000031:analogs & derivatives*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010879:Piperazines / Q000097:blood*
D010879:Piperazines; C037921:1-(2-pyrimidinyl)piperazine; D002065:Buspirone