The hydrophobic cationic cyanine dye inhibits oxidative phosphorylation by inhibiting ADP transport, not by electrophoretic transfer, into mitochondria.
The effect of the divalent cationic cyanine dye tri-S-C4(5) on oxidative phosphorylation in rat liver mitochondria was examined. The dye at about 100 n mols per mg mitochondrial protein inhibited state 3 respiration and ATP synthesis almost completely. However, it had no effect on submitochondrial particles, like other hydrophobic cations. The dye inhibited the transport of ADP into mitochondria mediated by the adenine nucleotide translocator. Thus, the inhibition of oxidative phosphorylation by the cationic dye was concluded to be due to its action on the adenine nucleotide translocator, not to its electrophoretic transfer into the inner space of mitochondria according to the inside-negative electrochemical potential.
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Y
Y
Shinohara
H
H
Nagamune
H
H
Terada
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004396:Coloring Agents; D004579:Electron Transport / Q000187:drug effects; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D051381:Rats; D013367:Submitochondrial Particles / Q000187:drug effects; D013844:Thiazoles / Q000494:pharmacology*; D014475:Uncoupling Agents / Q000494:pharmacology*
D004396:Coloring Agents; D013844:Thiazoles; D014475:Uncoupling Agents; D000244:Adenosine Diphosphate; C032150:butyl-platonin