Chloridite and amidite automated synthesis of oligodeoxyribonucleotides using amidine protected nucleosides.
Automated synthesis of oligodeoxyribonucleotides using in situ prepared methylchlorophosphite intermediates from amidine protected purine nucleosides is described. Phosphoramidite method using 1-methylimidazole trifluoromethanesulfonate as mediator is described.
Arnold
L
L
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Praha.
Tocík
Z
Z
Bradková
E
E
Hostomský
Z
Z
Smrt
J
J
D000578:Amidines; D002713:Chlorine; D003853:Deoxyribonucleosides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
D000578:Amidines; D003853:Deoxyribonucleosides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D002713:Chlorine