Two novel endogenous digoxin-like immunoreactive substances isolated from human plasma ultrafiltrate.
Two digoxin-like immunoreactive substances (DLIS 1 and DLIS-2) were isolated from human plasma by ultrafiltration, alkaline solvent extraction, and HPLC. Both inhibit canine Na, K-ATPase and cross-react with anti-digoxin antibodies. Their UV spectra are similar, with absorption peaks at 190 and 220 nm. FAB mass spectral analysis of both compounds yield a single dominant (M+H)+ component at m/z 532 otherwise, the fragmentation patterns are slightly different. The spectral data are consistent with the postulate that these compounds are related. Their identity as the natriuretic hormone is being investigated.
University of Washington, School of Medicine, Seattle 98195.
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Dasgupta
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Yeo
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Malik
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Sandu
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Ahmad
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M
Kenny
D002851:Chromatography, High Pressure Liquid; D004077:Digoxin / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D006440:Hemofiltration; D006801:Humans; D013058:Mass Spectrometry; D011863:Radioimmunoassay; D012997:Solvents; D014462:Ultrafiltration
D012997:Solvents; D004077:Digoxin