Minichromosomal variable surface glycoprotein genes and molecular karyotypes of Trypanosoma (Nannomonas) congolense.
Employing orthogonal-field-alternation gel electrophoresis (OFAGE), we have separated chromosome-sized DNA molecules from Trypanosoma (Nannomonas) congolense clones, the clones being derived from several distinct antigenic repertoires. Trypanosome clones that belong to a specific antigenic repertoire appear to have a chromosome pattern characteristic of that particular repertoire. Hybridization of the separated chromosomes with cloned DNA fragments encoding variable surface glycoproteins revealed the presence of two different T.(N.) congolense variable surface glycoprotein genes on mini-chromosomes (mc) and the modes by which these genes may be activated: one by duplicative and the other by non-duplicative activation.
P A
PA
Majiwa
J R
JR
Young
R
R
Hamers
G
G
Matthyssens
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014348:Trypanosoma congolense / Q000235:genetics*; D014643:Variant Surface Glycoproteins, Trypanosoma
D004274:DNA, Recombinant; D006023:Glycoproteins; D014643:Variant Surface Glycoproteins, Trypanosoma