Measurements of K+-driven Cl- uptake in thylakoids: inhibition of uptake by antibodies raised to the major polypeptides of the Cl(-)-efflux active particle(s).
The mechanism of a K+-driven Cl- accumulation against a concentration gradient was investigated by flow dialysis after addition of K+-Hepes. Non-specific chloride binding, measured in the presence of choline-Hepes, accounted for approximately 50% of the observed uptake in this system. The K+-Hepes-driven Cl- uptake was inhibited by poly-l-lysine and by two antibodies raised to the major polypeptides of the Cl(-)-efflux active particle. Poly-l-lysine had no effect on Cl- binding estimated with choline-Hepes.
Department of Biochemistry, Mount Sinai School of Medicine, City University of New York, New York 10029.
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D000906:Antibodies / Q000502:physiology; D001692:Biological Transport / Q000187:drug effects; D002713:Chlorine / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D006531:HEPES / Q000493:pharmacokinetics; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D011107:Polylysine / Q000493:pharmacokinetics; D011188:Potassium / Q000037:antagonists & inhibitors / Q000502:physiology*
D000906:Antibodies; D010455:Peptides; D011107:Polylysine; D002713:Chlorine; D011188:Potassium; D006531:HEPES