Monoclonal antibodies to bovine growth hormone potentiate effects of the hormone on somatomedin C levels and growth of hypophysectomized rats.
Bovine growth hormone (50 micrograms/day) elevated somatomedin C levels in plasma of hypophysectomized rats: the effect was seen 12 h after the first injection and was sustained throughout a 7-day treatment period. When monoclonal antibodies to bovine growth hormone were administered with the hormone the stimulation of somatomedin C levels was markedly enhanced, as were effects on body weight. Each one of a panel of 7 monoclonal antibodies potentiated the actions of the hormone on growth and somatomedin C levels, though the extent and pattern of potentiation varied considerably from one antibody to another. Effects on growth and somatomedin C levels correlated fairly well, though there were some discrepancies.
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, U.K.
M
M
Wallis
M
M
Daniels
K P
KP
Ray
J D
JD
Cottingham
R
R
Aston
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects; D002417:Cattle; D013006:Growth Hormone / Q000276:immunology / Q000494:pharmacology*; D007016:Hypophysectomy; D007334:Insulin-Like Growth Factor I / Q000097:blood*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013002:Somatomedins / Q000097:blood*
D000911:Antibodies, Monoclonal; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone