The role of detergent in labeling of Erysipelothrix rhusiopathiae bacteria.
The chelating agents oxine, acetylacetone, tropolone, and 2-mercaptopyridine 1-oxide were analysed for their suitability in labeling the bacterium Erysipelothrix rhusiopathiae (E.r.), strain B10, with 111In and 67Ga. The labeling conditions were improved. The bactericidal activity of the labeling process was investigated. The different chelates did not result in a significant difference in labeling yield. The addition of 0.01% detergent to the labeling buffer doubled the labeling yield up to 45%. Labeled bacteria were injected intravenously into rats. The percentage organ distribution was determined and compared with a control group.
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D000818:Animals; D002614:Chelating Agents; D003902:Detergents; D004888:Erysipelothrix / Q000302:isolation & purification; D004889:Erysipelothrix Infections / Q000382:microbiology; D005710:Gallium Radioisotopes; D007204:Indium; D008297:Male; D011868:Radioisotopes; D051381:Rats; D013501:Surface-Active Agents
D002614:Chelating Agents; D003902:Detergents; D005710:Gallium Radioisotopes; D011868:Radioisotopes; D013501:Surface-Active Agents; D007204:Indium