Plasminogen activator in bronchoalveolar fluid.
Plasminogen activator was purified from the soluble fraction of bronchoalveolar lavage fluid, and biological and immunological characteristics of the activator were examined by electrophoretic enzymography. The purified fraction showed a single band with a molecular weight of 53,000. The enzyme activity was eliminated in the presence of DFP and did not display fibrin affinity. Immunological tests revealed that the plasminogen activator reacted with IgG of antiurokinase but not with that of tissue-type plasminogen activator. However, the plasminogen activator cleaved S-2288 to a greater extent than S-2444 in contrast to urokinase.
Matsuo
O
O
Sakai
T
T
Bando
H
H
Okada
K
K
Nakajima
S
S
Takagi
O
O
Izaki
S
S
D000328:Adult; D000368:Aged; D001826:Body Fluids / Q000201:enzymology*; D001980:Bronchi / Q000201:enzymology*; D005260:Female; D006801:Humans; D007120:Immunochemistry; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009842:Oligopeptides; D010960:Plasminogen Activators / Q000276:immunology / Q000302:isolation & purification*; D011650:Pulmonary Alveoli / Q000201:enzymology*; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology
D009842:Oligopeptides; C018788:valyl-leucyl-lysine 4-nitroanilide; C028190:5-oxo-prolyl-glycyl-arginine-4-nitroanilide; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator