Inhibitory effect of 12-O-tetradecanoylphorbol 13-acetate on acid secretion by rat stomach in vivo.
The effect of 12-O-tetradecanoylphorbol 13-acetate (TPA) on acid secretion by rat stomach in vivo has been investigated to establish whether a previously determined inhibitory effect of TPA on aminopyrine accumulation by isolated parietal cells genuinely reflected a reduced acid secretion. Perfusion of 1 microM TPA through the lumen of the stomach had little effect on basal or carbachol-stimulated secretion, but inhibited secretion induced by histamine or pentagastrin. 4 alpha-Phorbol 12,13-didecanoate did not affect pentagastrin-stimulated secretion. It is concluded that TPA exerts a specific inhibitory effect on acid secretion in vivo, which parallels that found on aminopyrine accumulation in isolated parietal cells.
G P
GP
Shaw
P J
PJ
Hanson
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects; D006632:Histamine / Q000494:pharmacology; D008297:Male; D010418:Pentagastrin / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology; D010704:Phorbols / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010703:Phorbol Esters; D010704:Phorbols; C008876:phorbol-12,13-didecanoate; D006632:Histamine; D002217:Carbachol; D011493:Protein Kinase C; D010418:Pentagastrin; D013755:Tetradecanoylphorbol Acetate