[Distribution of subfractions of high-density lipoproteins during their separation according to hydrated density and particle size].
Distribution of high density lipoprotein (HDL) subfractions was studied by means of gradient ultracentrifugation depending on their hydration density and using electrophoresis in gradient polyacrylamide gel according to the particles size. 53 men were examined, where 43 patients were impaired with ischemic heart disease and with atherosclerosis of coronary arteries documented by angiography. In all the patients with ischemic heart disease and dislipoproteinemias distribution of the HDL particles was altered towards a decrease in amount of HDL2b particles; at the same time, loading with cholesterol of the HDL3 subfraction was lowered. The alterations in the subfraction spectrum and composition of HDL observed in the patients with the ischemia and coronary atherosclerosis appear to exhibit the atherogenous impairments in cholesterol elimination from arterial wall.
I A
IA
Shcherbakova
N V
NV
Perova
V A
VA
Metel'skaia
N V
NV
Anufrieva
A M
AM
Olfer'ev
D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006951:Hyperlipoproteinemias / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood / Q000302:isolation & purification*; D008722:Methods; D010316:Particle Size; D014461:Ultracentrifugation
D008075:Lipoproteins, HDL
Raspredelenie podfraktsiĭ lipoproteidov vysokoĭ plotnosti pri ikh razdelenii v sootvetstvii s gidratirovannoĭ plotnost'iu i razmerom chastits.