[The dose dependence of 15N-incorporation in organ proteins of newborn rats after pulse labeling with different tracers].
A short-chain 15N-peptide mixture characterized by an average chain length of 2.3 was obtained when 15N-labeled yeast protein has hydrolyzed enzymatically by thermitase from Thermoactinomyces vulgaris. Fifteen newborn Wistar-rats were given a single pulse of [15N]glycine. [15N]H4Cl and [15N]yeast protein-thermitasehydrolysate (YPTH) in a dosage of 50 mg 15N excess kg-1 by gastric tube. In comparison with [15N]glycine the 15N-incorporation rates of brain, muscle and liver were approximately 150% higher after [15N]YPTH-application. Uniform labeling, high 15N-enrichment, almost complete absorption, avoidance of imbalances and the low price make this tracer substance superior to other tracers conventionally used for organ labeling.
Wutzke
K D
KD
Klinik für Kinderheilkunde, Bereiches Medizin der Wilhelm-Pieck-Universität Rostock.
Zhukova
T P
TP
Sorokina
E G
EG
Plath
C
C
Richter
I
I
Heine
W
W
Friedrich
M
M
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D009587:Nitrogen Isotopes; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism*
D005656:Fungal Proteins; D009587:Nitrogen Isotopes; D010455:Peptides; D005998:Glycine
Untersuchungen zur Dosisabhängigkeit der N-Inkorporation in Organproteine neugeborener Ratten nach Pulsmarkierung mit verschiedenen Tracern.