Studies of metabolism of round spermatids: cytochalasin B binding to cell membrane in relation to glucose transport.
Cytochalasin B inhibited uptake of 2-deoxy-D-glucose in rat spermatids (Ki = 6.3 X 10(-7) M). This inhibition was reversible. The [3H]cytochalasin B was found to bind to spermatid plasma membranes at low drug concentrations. Lineweaver-Burk plots of binding data indicated that there was a class of high affinity sites (Kd = 2.9 X 10(-7) M). The results suggest that the high affinity cytochalasin B binding sites of spermatids are membrane-associated substances and are intimately related to the glucose transport system.
Nakamura
M
M
Department of Obstetrics and Gynecology, School of Medicine, Teikyo University, Tokyo, Japan.
Okinaga
S
S
Arai
K
K
D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D003571:Cytochalasin B / Q000493:pharmacokinetics / Q000494:pharmacology*; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008297:Male; D051381:Rats; D013087:Spermatids / Q000187:drug effects* / Q000378:metabolism
D003571:Cytochalasin B; D003847:Deoxyglucose; D005947:Glucose