Application of 1,1'-carbonyldiimidazole-activated matrices for the purification of proteins. IX. Dynamic multizoning effects in biospecific affinity chromatography on porous supports: evaluation of activation and ligand coupling effects with different support materials.
The activation of several porous polymeric gels and chemically bonded glycerylpropyl-silica-based packing materials with 1,1'-carbonyldiimidazole has been further investigated. The ligand coupling characteristics of these matrices following activation at low and high levels have been determined and the properties of the derived affinity chromatographic supports examined. In addition the dynamic capacities of several immobilised-ligand systems have been compared. The use of these different affinity chromatographic supports in assessing flow-rate and column residency effects is described.
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D000327:Adsorption; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D007093:Imidazoles; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D014357:Trypsin / Q000302:isolation & purification
D007093:Imidazoles; D011506:Proteins; C006900:N,N-carbonyldiimidazole; D014357:Trypsin