Enzyme-linked immunosorbent assay for detection of streptolysin O antibodies.
An enzyme-linked immumosorbent assay (ELISA), based upon the detection of streptolysin O antibodies in human sera, was developed. Disposable polystyrene tubes, sensitized with streptolysin O antigen, were used as the test vehicles. Corresponding antibodies, present in test sera, were detected by binding of the antibodies to goat anti-human immunoglobulin G conjugated to horseradish peroxidase. Demonstration of bound conjugate was accomplished by monitoring peroxidase activity spectrophotometrically at 450 nm, using 5-aminosalicylic acid as the indicator. A total of 97 human sera, previously analyzed by means of the anti-streptolysin O titration technique, were evaluated with the ELISA procedure. A direct quantitative relationship, found to be statistically significant, was demonstrated between Todd units and absorbance values obtained with ELISA.
Reitano
M
M
Pisano
M A
MA
Eriquez
L A
LA
D'Amato
R F
RF
D000907:Antibodies, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D013301:Streptolysins / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D013301:Streptolysins; C025964:streptolysin O