Loss of 3H-corticosterone binding capacity in hippocampal slices maintained in vitro.
Nuclear uptake of 3H-corticosterone was studied in hippocampal slices subjected to varying periods of preincubation before application of steroid. Uptake capacity declined rapidly toward nonspecific levels over the course of 3 hours from the time of cutting. Glucocorticoid receptors in the cytosol fraction of hippocampal slices also were found to decline, and Scatchard analysis indicated a loss of corticosterone binding sites rather than a change in binding affinity. These results are discussed in terms of the reported effects of corticosterone in vitro.
Halpain
S
S
Spanier
T
T
McEwen
B S
BS
D000315:Adrenalectomy; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003345:Corticosterone / Q000378:metabolism*; D003600:Cytosol / Q000032:analysis; D006624:Hippocampus / Q000032:analysis / Q000378:metabolism*; D066298:In Vitro Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000032:analysis
D011965:Receptors, Glucocorticoid; D003345:Corticosterone