[Actin and tubulin synthesis in monolayer and suspension cultures of murine L cells].
The rates of total protein, actin and tubulin synthesis were studied for monolayer (L-929) and suspension (LS) cultures of mouse L cell. Data on pulse 34S-label incorporation into the cellular protein pool show that LS characterized by a short cell cycle have, comparatively to L-929, higher rates of protein synthesis and phosphorylation. According to PAGE data, the level of actin and tubulin synthesis in suspension line exceeds that in monolayer one. The correlation between growth conditions, biosynthetic parameters and dynamics of cytoskeleton is discussed.
Margulis
B A
BA
Barlovskaia
V V
VV
Semenova
E G
EG
D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D007739:L Cells / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D010756:Phosphoric Acids / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D013462:Sulfur Radioisotopes; D014404:Tubulin / Q000096:biosynthesis*
D000199:Actins; D010756:Phosphoric Acids; D010761:Phosphorus Radioisotopes; D013462:Sulfur Radioisotopes; D014404:Tubulin; D008715:Methionine; C030242:phosphoric acid
Sintez aktina i tubulina v monosloĭnoĭ i suspenzionnoĭ kul'turakh kletok myshi L.