Inhibition of microtubule formation by uremic toxins: action mechanism and hypothesis about the active component.
We show in vitro inhibitory effect of a mixture of uremic toxins on tubulin 6S polymerization. It proves the existence of a direct interaction protein-toxin where micro-tubule associated proteins are not involved. A similar phenomenom could occur in uremic neuropathy. The action mechanism of this interaction is quite different from that of classical tubulin inhibitors: Vinca alcaloïdes and colchicine. Finally we hypothesize about the active molecule.
Braguer
D
D
Gallice
P
P
Monti
J P
JP
Murisasco
A
A
Crevat
A
A
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003078:Colchicine / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors; D008870:Microtubules / Q000187:drug effects; D013552:Swine; D014118:Toxins, Biological / Q000302:isolation & purification / Q000494:pharmacology*; D050257:Tubulin Modulators; D014511:Uremia / Q000378:metabolism*; D014748:Vinca Alkaloids / Q000494:pharmacology
D008869:Microtubule-Associated Proteins; D014118:Toxins, Biological; D050257:Tubulin Modulators; D014748:Vinca Alkaloids; C026865:uremia middle molecule toxins; D003078:Colchicine