Radioimmunoassay of gastrin in human saliva.
We have developed a personal procedure for the radioimmunoassay of gastrin (lower detection limit 3 pg/ml). Using 5-fold concentrated lyophilized salivary samples, we are able to detect the basal content of gastrin immunoreactivity in saliva of normal fasting people (mean +/- SD: 1.38 +/- 0.61 pg/ml) compared to plasma gastrin (mean +/- SD: 28.88 +/- 11.00 pg/ml). We found that after meal stimulus, the value of salivary as well as plasma gastrin concentration increased (the correlation coefficient r among salivary and plasma gastrin being 0.899; p less than 0.001). We believe that gastrin appears in a very low concentration in saliva and that it is not produced by the gland, but derives probably from the serum clearance.
Ingenito
A
A
Catelani
C
C
Mercaldo
A
A
d'Agata
A
A
Cappelli
G
G
Andreoli
F
F
D005755:Gastrins / Q000032:analysis* / Q000097:blood; D006801:Humans; D011863:Radioimmunoassay; D012463:Saliva / Q000032:analysis*
D005755:Gastrins