Choline uptake by glomerular synapses isolated from bovine cerebellar vermis.
[3H]Choline uptake was investigated in a highly enriched preparation of glomerular particles isolated from bovine cerebellar vermis. Kinetic analysis indicates that a high-affinity choline uptake system with a relatively low maximum uptake velocity is present. At a substrate concentration of 10(-7) M, [3H]choline uptake was shown to be sodium-dependent, hemicholinium-sensitive and non-reactive to increasing concentrations of unlabeled choline. These findings support the presence of a high-affinity choline uptake system in cerebellar glomeruli and would seem to be consistent with the suggestion that a small proportion of mossy fibers are cholinergic in this brain region.
Terrian
D M
DM
Noisin
E L
EL
Thomas
W E
WE
D000818:Animals; D002417:Cattle; D002531:Cerebellum / Q000033:anatomy & histology / Q000378:metabolism*; D002794:Choline / Q000378:metabolism*; D002799:Cholinergic Fibers; D006426:Hemicholinium 3 / Q000494:pharmacology; D007700:Kinetics; D012964:Sodium / Q000502:physiology
D006426:Hemicholinium 3; D012964:Sodium; D002794:Choline