Androgen dependence of the Dunning R3327G cell line in monolayer culture.
For optimal application of new treatment strategies for prostate cancer, the basic biologic effects of androgens on cell kinetics and DNA synthesis require detailed examination. An androgen-responsive prostate cancer cell line in monolayer culture provides a means to study the biochemical mechanisms mediating hormonal stimulation of cell proliferation. We chose to evaluate the proliferative response of the Dunning R3327G tumor cell line (Du-G cells) to 5 alpha-dihydrotestosterone (DHT) in monolayer culture. The DU-G cells grew more rapidly in the presence of increasing concentrations of DHT in the range of 10(-8)-10(-5) M than with vehicle control. At 10(-7) M DHT, 3H-thymidine incorporation increased from 400 +/- 34 counts/min/well to 751 +/- 77 (p less than .01). Effects of DHT were maximal when a plating density of 10,000 cells/well was employed. Androgen effects on cellular growth were reproducible but were limited in magnitude. Rapid metabolism of DHT in culture did not explain this phenomenon. Du-G cells were not completely dependent on androgen, since cells continued to grow in media containing less than 10(-11) M dihydrotestosterone and hydroxyflutamide.
Department of Reproductive Endocrinology, Hôpital Necker, France.
R J
RJ
Santen
I
I
Mowszowicz
M C
MC
Portois
P
P
Mauvais-Jarvis
D000726:Androgen Antagonists / Q000494:pharmacology; D000728:Androgens / Q000097:blood; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D013196:Dihydrotestosterone / Q000494:pharmacology; D005485:Flutamide / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology*; D009919:Orchiectomy; D011471:Prostatic Neoplasms / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000726:Androgen Antagonists; D000728:Androgens; D013196:Dihydrotestosterone; C014290:hydroxyflutamide; D005485:Flutamide