The influence of serum substitute Ultroser G in toxicological evaluations in mammalian cells in vitro.
The influence of serum substitute Ultroser G on the toxicity of the antiparasitic ivermectin was evaluated in CHO and IB-RS-2 pig kidney cells. The growth of both cell lines was slightly slower in medium with Ultroser G than with serum. However, the growth of both CHO and IB-RS-2 cells exposed to 5 and 10 micrograms/ml ivermectin decreased to a higher extent when they were maintained in medium with Ultroser G than with serum. The treatment with 20 micrograms/ml caused the death of the cells cultured with the serum substitute within 6 hr; the growth of CHO cells ceased after 24 hr in medium with serum, while IB-RS-2 cells were still slowly growing after 72 hr. The morphological changes caused by the drug were observed under light microscope.
Instituto Biológico, Sào Paulo, Brazil.
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Rodrigues
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Mattei
D000818:Animals; D001802:Blood Substitutes / Q000627:therapeutic use*; D002470:Cell Survival / Q000187:drug effects; D066298:In Vitro Techniques; D007559:Ivermectin / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects*; D009930:Organic Chemicals; D013552:Swine
D001802:Blood Substitutes; D009930:Organic Chemicals; C044609:Ultroser G; D007559:Ivermectin