Effect of elevated temperature on catalase and superoxide dismutase during maize development.
Seeds of the inbred maize lines, W64A, R6-67, and D10, were germinated and grown at 25 degrees, 35 degrees, or 40 degrees C for up to 10 days. The catalase activity in scutella of W64A seedlings grown at 40 degrees C was slightly lower than that in seedlings grown at 25 degrees C. The total superoxide dismutase activity in scutella was lower in seedlings grown at 40 degrees C than in those grown at 25 degrees C during the first 3 days of germination, but thereafter was not significantly different at these temperatures. The high-catalase mutant lines, R6-67 and D10, grown at 40 degrees C exhibited a developmental pattern of catalase activity that was severalfold lower than that seen in seedlings grown at 25 degrees C. The decrease in catalase activity in R6-67 seedlings grown at 40 degrees C was correlated with lower amounts of CAT-2 protein, which is normally present at significantly high levels in this line. The application of a catalase synthesis inhibitor revealed that the low levels of CAT-2 in R6-67 grown at 40 degrees C were due to slightly higher degradation rates and a significant drop in the rate of catalase protein synthesis.
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D002374:Catalase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D005786:Gene Expression Regulation; D006358:Hot Temperature; D007527:Isoenzymes / Q000378:metabolism; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D007527:Isoenzymes; D002374:Catalase; D013482:Superoxide Dismutase