A cell-cycle constraint on the regulation of gene expression by platelet-derived growth factor.
In density-arrested monolayer cultures of Balb/c 3T3 cells, platelet-derived growth factor (PDGF) stimulates expression of the c-myc and c-fos proto-oncogenes, as well as the functionally uncharacterized genes, JE, KC, and JB. These genes are not coordinately regulated. Under ordinary conditions, c-fos, JE, KC, and JB respond to PDGF only when the cells are in a state of G0 growth arrest at the time of PDGF addition. The c-myc gene is regulated in opposition to the other genes, responding best to PDGF in cycling cultures.
Division of Medicine, Dana-Farber Cancer Institute, Boston, MA 02115.
B J
BJ
Rollins
E D
ED
Morrison
C D
CD
Stiles
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D007399:Interphase; D051379:Mice; D008807:Mice, Inbred BALB C; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011519:Proto-Oncogenes / Q000187:drug effects*; D014158:Transcription, Genetic / Q000187:drug effects*
D010982:Platelet-Derived Growth Factor