Biochemical and morphological characterization of primary kidney cell cultures from beige mutant mice.
Primary kidney cultures from adult beige-J (bgJ/bgJ) mice were selected for epithelial cell growth using D-valine medium. After 2 weeks of attachment and proliferation in vitro, the cells form a confluent or nearly confluent monolayer that retains several phenotypic characteristics of the beige-J mutant. These include large, multilamellar inclusion bodies that are apparently dysmorphic lysosomes, and higher concentrations of neutral glycosphingolipids and dolichols than control cells. beta-Glucuronidase activity, used as a lysosomal enzyme marker, is not elevated in beige-J-cultured kidney cells compared with controls, as it is in the intact kidney. The high levels of beta-glucuronidase activity in both control and mutant cells may mask expression of this difference in vitro. The action of the beige-J mutation in kidney cells is thought to be due to a block in exocytosis that results in the accumulation of abnormal lysosomes and their components. The maintenance of the beige phenotype in vitro indicates that the mutation is not suppressed in primary kidney cell cultures. The expression of the beige phenotype in vitro should be useful for studies concerning the primary lesion of this mutation.
Department of Biochemistry, Eunice Kennedy Shriver Center for Mental Retardation, Inc., Waltham, Massachusetts.
T A
TA
Lyerla
S K
SK
Gross
T B
TB
Shea
P F
PF
Daniel
R H
RH
McCluer
D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells; D005966:Glucuronidase / Q000378:metabolism; D007668:Kidney / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D012016:Reference Values
D005966:Glucuronidase