Liquid storage at 4 degrees C of previously frozen red cells.
Fresh human blood was collected in citrate-phosphate-dextrose, frozen by a high-glycerol technique, and stored at -80 degrees C. The red cells were thawed, deglycerolized, and resuspended in a final wash solution, ADSOL (Fenwal Laboratories), or an additive solution (AS) containing glucose, adenine, mannitol, and phosphate. The cells were then stored at 4 to 6 degrees C for 21 days and assayed weekly for adenosine triphosphate and 2,3 diphosphoglycerate, pH, glucose use, and lysis. AS and, to a lesser extent, ADSOL produced metabolic profiles similar to or better than profiles of cells not frozen and stored in commercially available additive solutions. AS offers a potential post-thaw preservative solution for red cells that would greatly increase the flexibility and reduce the expense of using frozen blood. A sterile post-thaw storage capability will make the stockpiling of frozen red cells a practical concept for both military and civilian blood banks.
Moore
G L
GL
Letterman Army Institute of Research, Presidio of San Francisco, California.
Ledford
M E
ME
Mathewson
P J
PJ
Hankins
D J
DJ
D001793:Blood Preservation; D003080:Cold Temperature; D004912:Erythrocytes; D005069:Evaluation Studies as Topic; D005615:Freezing; D006801:Humans