Heavy metal-induced gene expression in fish and fish cell lines.
Two isoforms of metallothionein (MT) have been isolated from rainbow trout livers following CdCl2 injections. These MTs have been identified by standard procedures and appear to be similar to mammalian MTs. Total RNA from such induced livers was shown to contain high levels of MT-mRNA activity when translated in cell free systems. This activity was demonstrated to be in the 8 to 10S region of a sucrose gradient. The RNA fractions also showed homology to a mouse MT-I cDNA probe. The exposure of rainbow trout hepatoma (RTH) cells to various concentrations of CdCl2 and ZnCl2 induced the expression of MT and MT-mRNA. Exposure of Chinook salmon embryonic (CHSE) cells to these metals, however, did not result in MT synthesis, suggesting that the MT genes have not become committed to transcription. Instead, an unknown low molecular weight (MW = 14 kDa) protein was induced. This metal-inducible protein (MIP) was capable of binding 109Cd and was stable to heating, while the binding of the metal to this protein was not. These characteristics have been reported for a protein induced in rainbow trout liver following environmental exposure to cadmium. We suggest that both MT and MIP may function in detoxification of heavy metals.
J
J
Price-Haughey
K
K
Bonham
L
L
Gedamu
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D008668:Metallothionein / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012474:Salmon / Q000235:genetics*; D012487:Salmonidae / Q000235:genetics*; D013997:Time Factors; D014337:Trout / Q000235:genetics*; D015032:Zinc / Q000494:pharmacology
D000596:Amino Acids; D012333:RNA, Messenger; C004203:cadmium-binding protein; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc