The diagnosis of gram-negative bacillary pneumonia in an animal model using a competitive ELISA technique to detect the presence of lipid A.
Gram-negative bacillary pneumonias represent a major problem in hospitalized patients. A major research interest has been towards the development of rapid and accurate diagnostic procedures for these infections. An ELISA procedure for the detection of lipid A in pulmonary lavage fluid has been developed. Using the quantitation of lipid A, an antigen common to all clinically important gram-negative bacilli, as a marker for gram-negative bacilli, the presence or absence of pneumonia caused by these organisms could be demonstrated in laboratory animals. Additionally, by quantitating the lipid A associated with the lavageable pulmonary cell population, a measure of bacterial lung clearance could be obtained.
Campbell
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Woods
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D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008050:Lipid A / Q000032:analysis*; D008954:Models, Biological; D011014:Pneumonia / Q000175:diagnosis*; D011650:Pulmonary Alveoli / Q000032:analysis; D011817:Rabbits; D007507:Therapeutic Irrigation
D008050:Lipid A