Fatty acyl composition of the major lipid classes of HDL2 and HDL3 of human serum.
High density lipoprotein subfractions 2 and 3 were isolated from the sera of normolipidaemic males by rate zonal ultracentrifugation. The fatty acyl compositions of their major lipid classes--phospholipids, cholesteryl esters and triacylglycerols--were determined. The average chain length and degree of unsaturation of the fatty acyl components of the phospholipids and cholesteryl esters of the HDL3 subfraction were found to be significantly higher than those of the HDL2 subfraction. The triacylglycerol moieties of the HDL3 subfraction had a higher proportion of unsaturated fatty acyl components than HDL2, but there was no difference in the average chain length between the two subfractions. These results are not consistent with an equilibration of the lipid components between the two HDL subfractions, as would be expected from the results of tracer studies. A role for the fatty acyl composition of high density lipoproteins in the determination of high density lipoprotein subfraction distribution is proposed.
M M
MM
Devitt
D R
DR
Headon
D000328:Adult; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D002788:Cholesterol Esters / Q000097:blood; D005227:Fatty Acids / Q000097:blood*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D008297:Male; D010743:Phospholipids / Q000097:blood; D014280:Triglycerides / Q000097:blood
D002788:Cholesterol Esters; D005227:Fatty Acids; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D010743:Phospholipids; D014280:Triglycerides