Fitting fluorescence emission spectra of probes bound to biological membranes.
We show that fluorescence emission spectra for molecules containing the dansyl fluorophor can be accurately described as skewed Gaussians, and that spectra for dansyl probes bound to biological membranes can be resolved using least-squares techniques into two components, representing probe bound to the lipid and protein sites in the membrane.
Rooney
E K
EK
Lee
A G
AG
D001665:Binding Sites; D001699:Biometry; D003619:Dansyl Compounds; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008566:Membranes / Q000378:metabolism*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
D003619:Dansyl Compounds; D008563:Membrane Lipids; D008565:Membrane Proteins