Interaction of rat liver glucocorticoid receptor with lectins: is the glucocorticoid receptor a glycoprotein?
Although glucocorticoid receptors have been extensively studied in a variety of tissues, the precise nature of the receptor protein still remains unknown. To further characterize this protein we assessed the effects of various lectins on [3H]dexamethasone binding to prepurified preparations of rat liver glucocorticoid receptor. Among the lectins tested only Ulex europeus and Lens culinaris induced a concentration-related decrease in [3H]dexamethasone binding. Following Ulex europeus or Lens culinaris exposure Scatchard analysis showed that these lectins led to a 3-fold reduction in receptor affinity without influencing the concentration of binding sites. These results provide new experimental evidence that rat liver glucocorticoid receptor would possess alpha-L-fucosyl and alpha-D-mannopyranoside residues in close proximity to the glucocorticoid receptor binding domain.
Blanchardie
P
P
Lustenberger
P
P
Denis
M
M
Orsonneau
J L
JL
Bernard
S
S
D000818:Animals; D003907:Dexamethasone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins / Q000494:pharmacology*; D008099:Liver / Q000032:analysis; D008297:Male; D037121:Plant Lectins; D011479:Protamines / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D011479:Protamines; D011965:Receptors, Glucocorticoid; C036850:Ulex europaeus lectins; C033619:lentil lectin; D014316:Tritium; D003907:Dexamethasone