Modulation of protein expression in murine bone marrow cells following in-vivo cytosine arabinoside treatment.
The technique of SDS-PAGE followed by autoradiography was applied to study the changes in protein synthesis occurring in murine bone marrow cells in response to AraC administration. It was found that following AraC injection the medullary population undergoes pronounced changes in protein synthesis. Marked increases were noted in the 20, 28 and 70 kD protein labelling, concomitantly with CFU-S stimulating activity of the AraC treated mice bone marrow conditioned medium.
Wdzieczak-Bakala
J
J
Lenfant
M
M
Frindel
E
E
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism*; D003561:Cytarabine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D014176:Protein Biosynthesis; D013462:Sulfur Radioisotopes; D013997:Time Factors
D013462:Sulfur Radioisotopes; D003561:Cytarabine; D004247:DNA; D008715:Methionine