Separation of purine bases, nucleosides and nucleotides by a column-switching technique combining reversed-phase and anion-exchange high-performance liquid chromatography.
An on-line two-stage column chromatographic technique is described which combines reversed-phase and anion-exchange chromatography for the separation of purine nucleic acid components. The elution program applied, consisting of two gradient programmes, provides a separation of bases and nucleosides on the octadecyl silica column and a separation of the nucleotides on the anion-exchange column to which they have been switched at the beginning of the elution. This method is easy to modify for special problems and can be used when establishing a complete profile of purines.
Lang
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HR
Rizzi
A
A
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D007202:Indicators and Reagents; D011684:Purine Nucleosides / Q000302:isolation & purification*; D011685:Purine Nucleotides / Q000302:isolation & purification*; D011687:Purines / Q000302:isolation & purification*
D007202:Indicators and Reagents; D011684:Purine Nucleosides; D011685:Purine Nucleotides; D011687:Purines