Interaction of tin and zinc in some processes of heme biosynthesis in rabbits.
Female rabbits (2.5-3.0 kg) were divided into five groups. Group I (control) received ip 0.9% NaCl, every day for 5 days; group II received ip SnCl2 (dose of 2 mg Sn/kg) every day for 5 days; group III was given single sc injection of ZnSO4 (dose of 50 mg Zn/kg); group IV, a single sc injection of ZnSO4 followed by a single ip injection of SnCl2 on the next day; and group V received a single ip injection of SnCl2 followed next day by a single sc injection of ZnSO4. Eighteen hours after the first dose of SnCl2 an inhibition of delta-aminolevulinic acid dehydratase (ALA-D) by 60% was found in the blood of group II, while increases of urinary excretion of coproporphyrin (CP-U) and delta-aminolevulinic acid (ALA-U) with respect to the control group took place not earlier than after the fourth dose of SnCl2 (correlation coefficients, respectively: ALA-D/ALA-U r = 0.91; CP-U/ALA-D r = 0.70; CP-U/ALA-U r = 0.81). The single dose of ZnSO4 resulted in an increase of ALA-D by about 60% while administration of SnCl2 to the animals in group IV decreased this value down to physiologic levels. Then an increased tendency was noted again for the activity of this enzyme. Administration of SnCl2 and ZnSO4 (group V) resulted in a decrease of ALA-D activity and in an increase in CP-U.
Zareba
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G
Chmielnicka
J
J
Kustra
G
G
D000622:Aminolevulinic Acid / Q000652:urine; D000818:Animals; D003306:Coproporphyrins / Q000652:urine; D004347:Drug Interactions; D005260:Female; D006418:Heme / Q000096:biosynthesis*; D007854:Lead / Q000097:blood; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors / Q000097:blood; D011817:Rabbits; D014001:Tin / Q000009:adverse effects / Q000494:pharmacology*; D015032:Zinc / Q000009:adverse effects / Q000494:pharmacology*
D003306:Coproporphyrins; D007854:Lead; D006418:Heme; D014001:Tin; D000622:Aminolevulinic Acid; D000623:Porphobilinogen Synthase; D015032:Zinc