[Isolation of alpha-latrotoxin using high-performance liquid chromatography methods].
alpha-Latrotoxin, a main toxic component of the Latrodectus mactans tredecimguttatus venom is a large polypeptide with molecular weight of 130 KDa. A rapid method is suggested for isolating this protein using high-effective liquid chromatography on chromatograph FPLC ("Pharmacia", Sweden). The isolated protein does not differ from the previously described alpha-latrotoxin in the main physicochemical parameters as well as in physiological properties.
E I
EI
Grebinozhko
A N
AN
Nikolaenko
D000818:Animals; D001180:Arthropod Venoms / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D007928:Lethal Dose 50; D051379:Mice; D013111:Spider Venoms / Q000302:isolation & purification* / Q000633:toxicity
D001180:Arthropod Venoms; D013111:Spider Venoms; C020763:alpha-latrotoxin
Vydelenie alpha-latrotoksina s pomoshch'iu metodov vysokoéffektivnoĭ zhidkostnoĭ khromatografii.