Isolation of oligonucleosomes from active chromatin using HMG17-specific monoclonal antibodies.
We report the preparation of HMG17-containing oligonucleosomes from chicken embryos and from liver and oviduct of laying hens. Monoclonal antibodies against HMG17 were used for their isolation. An unusual size distribution with respect to their repeat number was observed. The oligonucleosomes of repeat number up to N6 were highly enriched for DNA of the vitellogenin II gene (liver) and for DNA of the ovalbumin and lysozyme genes (oviduct).
Dorbic
T
T
Wittig
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000648:ultrastructure; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D005260:Female; D006609:High Mobility Group Proteins / Q000276:immunology*; D008099:Liver / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D009707:Nucleosomes / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D002843:Chromatin; D006609:High Mobility Group Proteins; D009707:Nucleosomes