Some steric aspects of synthesis of oligoribonucleotides by phosphoroamidite approach on solid support.
The influence of 2'-O-substituents (i.e. methyl, tetrahydropyranyl, t-butyldimethylsilyl) on the chemical synthesis of oligoribonucleotides by the phosphoroamidite approach on solid support is studied and compared with 2'-deoxynucleosides. Strong dependence on the steric hindrance of 2'-substituents both for the 3'-phosphitylation and condensation steps is described.
Kierzek
R
R
Institute of Bio-organic Chemistry, Polish Academy of Sciences, Poznan.
Rozek
M
M
Markiewicz
W T
WT
D007202:Indicators and Reagents; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis*; D013329:Structure-Activity Relationship
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds