Uncoupling effects of local anesthetics on rat liver mitochondria.
We demonstrate in this paper that bupivacaine, a local anesthetic, can act alone as an uncoupler of rat liver mitochondria. It stimulates state 4 respiration, induces a swelling in potassium acetate (in the presence of valinomycin), and collapses the transmembrane potential. Lidocaine, another local anesthetic, requires the presence of a lipophilic anion such as TPB- to produce the same effects. TPB- can also reinforce the action of bupivacaine. These differences in action of the two local anesthetics can be explained by the difference in the liposolubility.
Dabadie
P
P
Université Bordeaux II, CNRS, Bordeaux, France.
Bendriss
P
P
Erny
P
P
Mazat
J P
JP
D000818:Animals; D002045:Bupivacaine / Q000494:pharmacology*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D008012:Lidocaine / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D008933:Mitochondrial Swelling / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014475:Uncoupling Agents; D014634:Valinomycin / Q000494:pharmacology
D014475:Uncoupling Agents; D014634:Valinomycin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D008012:Lidocaine; D002045:Bupivacaine