Gas chromatography-mass spectrometric analysis of salivary testosterone with reference to diethylstilboestrol-treated prostatic cancer patients.
Testosterone has been quantified in saliva by gas chromatography-mass spectrometry with selected ion monitoring of the t-butyldimethylsilyl ether (TBDMS) of the oxime derivative. High specificity of instrumental analysis has been complemented by immunoadsorption for extraction of the analyte. The sensitivity of the detection is approx. 4 pmol/l and the precision of quantification is 3.4% CV for a value of 160 pmol/l and 11% for a value of 25 pmol/l. Female subjects had salivary concentrations of testosterone of 14-52 pmol/l, while testosterone was not detected in the saliva of prostatic cancer patients treated with diethylstilboestrol (DES).
Gould
V J
VJ
Turkes
A O
AO
Gaskell
S J
SJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D004054:Diethylstilbestrol / Q000627:therapeutic use*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D011485:Protein Binding; D011863:Radioimmunoassay; D012463:Saliva / Q000378:metabolism*; D013739:Testosterone / Q000097:blood / Q000378:metabolism*
D013739:Testosterone; D004054:Diethylstilbestrol