Distribution of 8 alpha-hydroxyriboflavin in rat organs.
From a survey of flavin compounds using a high performance liquid chromatograph equipped with a fluorescence detector, 8 alpha-hydroxyriboflavin, its derivative, riboflavin, FMN, and FAD were found in rat organs. The derivative of 8 alpha-hydroxyriboflavin was purified from the kidney sequentially by DEAE column chromatography, paper chromatography, and high performance liquid chromatography, and was determined to be 8 alpha-hydroxyriboflavin 5'-phosphate. The amount of 8 alpha-hydroxyriboflavin in the brain, heart, liver, and kidney of the rat was calculated to be 0.010, 0.026, 0.172, and 0.103 nmol per g of wet tissue, respectively. The amount of 8 alpha-hydroxyriboflavin 5'-phosphate in the brain, heart, and liver was less than 0.002 nmol, while that in the kidney was 0.330 nmol, per g of wet tissue. On the other hand, we could not detect 7 alpha-hydroxyriboflavin in the organ extracts, even though it was excreted into human urine along with 8 alpha-hydroxyriboflavin. This is the first reported occurrence of 8 alpha-hydroxyriboflavin and 8 alpha-hydroxyriboflavin 5'-phosphate in higher animal organs.
H
H
Ohkawa
N
N
Ohishi
K
K
Yagi
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005415:Flavins / Q000032:analysis; D005453:Fluorescence; D007668:Kidney / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000032:analysis
D005415:Flavins; C038139:8-hydroxymethylriboflavin; C038138:7-hydroxymethylriboflavin; D012256:Riboflavin