Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2 alpha kinase.
The VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of host cell and viral mRNAs late after infection. The growth of a viral mutant that is unable to produce the RNA is inhibited by interferon, while wild-type virus is not affected. VAI RNA prevents activation of the interferon-induced P1/eIF-2 alpha kinase. This inhibition can be reproduced in extracts of interferon-treated cells where purified VAI RNA prevents activation of latent kinase by double-stranded RNA.
Kitajewski
J
J
Schneider
R J
RJ
Safer
B
B
Munemitsu
S M
SM
Samuel
C E
CE
Thimmappaya
B
B
Shenk
T
T
D000260:Adenoviruses, Human / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004622:Embryo, Mammalian; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007668:Kidney; D010766:Phosphorylation; D014176:Protein Biosynthesis; D047428:Protein Kinase Inhibitors; D012330:RNA, Double-Stranded / Q000494:pharmacology; D012342:RNA, Small Nuclear / Q000502:physiology*; D012367:RNA, Viral / Q000502:physiology*; D019892:eIF-2 Kinase
D007370:Interferon Type I; D047428:Protein Kinase Inhibitors; D012330:RNA, Double-Stranded; D012342:RNA, Small Nuclear; D012367:RNA, Viral; D019892:eIF-2 Kinase