Histogenesis of UV light-induced blister formation.
Histological examination of ultraviolet light (UVL)-induced blister formation in guinea pig skin is reported. Light and electron-microscopic examination was performed on skin exposed to single and multiple exposures of a large dose of UVB. In light microscopy, intracellular edema was prominent, especially in the lower part of the epidermis, where it developed into a blister. It seemed to be a subepidermal bulla. The PAS-positive basement membrane was observed at the floor of the blister. The positive Nikolsky's sign seemed to be due to a separation between the degenerated and regenerated epidermis. Electron microscopy revealed a marked intercellular as well as intracellular edema and well-preserved desmosomes. The basal lamina was always located at the floor of the blister. The difference in UVL-induced blister formation between normal and pathological conditions is discussed.
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
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T
Horio
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Horiguchi
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Danno
D000818:Animals; D001768:Blister / Q000209:etiology* / Q000473:pathology; D004307:Dose-Response Relationship, Radiation; D004817:Epidermis / Q000473:pathology / Q000528:radiation effects / Q000648:ultrastructure; D006168:Guinea Pigs; D008854:Microscopy, Electron; D011833:Radiation Injuries, Experimental; D014466:Ultraviolet Rays / Q000009:adverse effects*