In vivo chemosensitivity testing in patients with gynecologic malignancies and nude mouse xenografts by monitoring cell kinetic parameters and DNA distribution patterns: a preliminary report.
Using flow cytometry (FCM) and autoradiography we have evaluated changes in DNA-ploidy patterns as well as cell-cycle perturbations after chemotherapy in 12 patients with accessible tumors and 22 nude mice xenografts. Gynecologic malignancies growing as nude mouse xenografts serve as an experimental model to study the effect of chemotherapy at the cellular level. Excellent correlation was found between fine needle aspiration (FNA) and biopsy material for either FCM or autoradiography. It now appears possible to study the effects of chemotherapy in the patient as well as the animal model using serial FNAs as a microsampling technique.
Sevin
B U
BU
Pollack
A
A
Averette
H E
HE
Ramos
R
R
Greening
S E
SE
Evans
D
D
D000230:Adenocarcinoma / Q000032:analysis / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001706:Biopsy; D002453:Cell Cycle; D002945:Cisplatin / Q000008:administration & dosage; D003537:Cystadenoma / Q000032:analysis / Q000188:drug therapy / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D005833:Genital Neoplasms, Female / Q000032:analysis / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000032:analysis / Q000188:drug therapy / Q000473:pathology
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D002945:Cisplatin