Cartilage proteoglycan aggregates. The link protein and proteoglycan amino-terminal globular domains have similar structures.
Cartilage proteoglycan aggregates contain two components (proteoglycan monomer and link protein) which interact with each other and with hyaluronic acid. Data from amino acid sequence analysis are presented that shows that a domain of the proteoglycan, the hyaluronic acid binding region, which interacts with link protein and hyaluronic acid is very similar to link protein in terms of its primary structure. However, the pattern of glycosylation in the hyaluronic acid binding region is different from that found in link protein. After removal of N-linked oligosaccharides, the tryptically prepared hyaluronic acid binding region from rat chondrosarcoma has a mass by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of 43 +/- 2 kDa. The COOH-terminal two-thirds of rat chondrosarcoma link protein, starting at residue 105, has 41.3% identity with a similar region in the hyaluronic acid binding region. We show that, in addition to the hyaluronic acid binding region, proteoglycan contains another region with similarity to the two repeating loop structures in the COOH-terminal two-thirds of link protein. This presumably corresponds to the second globular domain reported in rotary shadowing studies of cartilage proteoglycans. We have deduced the positions of all of the disulfide bonds in the hyaluronic acid binding region and find them to be in the same positions as would be expected from comparison of these sequences with link protein.
Department Medicine, University of Alabama, Birmingham 35294.
P J
PJ
Neame
J E
JE
Christner
J R
JR
Baker
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002356:Cartilage / Q000032:analysis*; D002645:Chickens; D006820:Hyaluronic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011509:Proteoglycans / Q000032:analysis*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000032:analysis
D000596:Amino Acids; D011509:Proteoglycans; D006820:Hyaluronic Acid