Autoradiographic localization of adenosine A1 receptors in rat brain using [3H]XCC, a functionalized congener of 1,3-dipropylxanthine.
Quantitative autoradiography was used to visualize the anatomical distribution of adenosine receptors labeled by the carboxylic acid congener of 1,3-dipropylxanthine, [3H](8-(p-carboxymethyloxy)phenyl-1,3-dipropylxanthine)([3H]XCC ) in the rat brain. [3H]XCC was observed to specifically bind to rat brain sagittal sections in a heterogeneous pattern. Saturation experiments revealed that [3H]XCC binds with nanomolar affinity to 20-microns frozen tissue sections with the highest binding densities occurring in the hippocampus and cerebellum. Both the binding characteristics and regional receptor distribution obtained with [3H]XCC demonstrate the potential usefulness of this new ligand in the study of adenosine A1 receptors.
Jarvis
M F
MF
Research Department, CIBA-GEIGY Corporation, Summit, NJ 07901.
Jacobson
K A
KA
Williams
M
M
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000378:metabolism*; D014316:Tritium; D014970:Xanthines / Q000378:metabolism*
D011983:Receptors, Purinergic; D014970:Xanthines; D014316:Tritium; C054469:8-(4-carboxymethyloxy)phenyl-1,3-dipropylxanthine