Fibrinolysis in ascitic fluid: isolation and characterization of fibrin derivatives, their interaction with albumin.
A method for the preparative isolation of high molecular weight fibrin degradation products (XDP) from ascitic fluid of patients with advanced ovarian cancer is described. By non-reduced and reduced PAA-Gel electrophoresis we could demonstrate that high molecular weight fibrin derivatives exist as E-complexes. A similarity of the polypeptide chain composition from in vitro samples and ascitic fluid could be shown. Immunoadsorption with anti-albumin followed by Westernblotting with anti-fibrinogen-demonstrated the existence of XDP/albumin-associates in ascitic fluid.
Frauenklinik der Technischen Universität München, F.R.G.
O
O
Wilhelm
R
R
Hafter
H
H
Graeff
D001202:Ascitic Fluid / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000302:isolation & purification*; D005342:Fibrinolysis; D006801:Humans; D008970:Molecular Weight; D012709:Serum Albumin / Q000378:metabolism*
D005338:Fibrin Fibrinogen Degradation Products; D012709:Serum Albumin