Upper aerodigestive neoplasm perpetuated in the nude mouse.
The ability to study and characterize squamous cell cancer of the upper aerodigestive system would be greatly facilitated by an in vivo model. The subsequent opportunity to observe cellular kinetics, membrane antigenicity and hence, potential response to chemotherapy, immunotherapy, radiotherapy, or a combination of these treatment modalities would most likely have early and significant clinical relevance. A multidisciplinary team of basic scientists and clinicians have developed a nude mouse colony for such investigational research. Melanoma and pulmonary squamous and pulmonary adenocarcinoma have been grown and successfully transferred. In addition, head and neck squamous cell carcinoma from multiple sites have also been successfully colonized. We present our experience with this interesting in vivo model and discuss problems in creating a nude mouse colony, techniques of successful tumor inoculation, ongoing maintenance of successful cell lines, and theoretical advantages for potential clinical investigations using this dynamic biologic system.
Fantozzi
R
R
Bone
R
R
Fernsten
P
P
Walker
L
L
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002460:Cell Line; D004195:Disease Models, Animal; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D008545:Melanoma / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010039:Otorhinolaryngologic Neoplasms / Q000473:pathology*; D014183:Transplantation, Heterologous