Supercoiled induced transition to the Z-DNA conformation affects the ability of a d(CG/GC)12 sequence to be organized into nucleosome-cores.
Nucleosome-cores were reconstituted by the salt-dialysis method onto closed circular pDHg16 DNA which contains a d(CG/GC)12 sequence. Alternating d(CG/GC)n sequences form left-handed Z-DNA readily when contained in negatively supercoiled DNA. We have investigated the ability of the d(CG/GC)12 sequence to be organized into nucleosome-cores when stabilized as Z-DNA through negative supercoiling. We have found that nucleosome assembly at the d(CG/GC)12 insert is prevented when the sequence is stable in the Z-conformation but it is not affected at all when the sequence adopts the right-handed B-form.
Grupo de Química Macromolecular, Centro de Investigación y Desarrollo del CSIC, Barcelona, Spain.
J M
JM
Casasnovas
F
F
Azorín
D000818:Animals; D002645:Chickens; D004247:DNA; D004278:DNA, Superhelical; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000648:ultrastructure*
D004278:DNA, Superhelical; D009707:Nucleosomes; D004247:DNA