The estimation of Hb A2 in the presence of Hb C or Hb E by reverse phase high performance liquid chromatography.
Although Hb-C may be separated from Hb A2 by some ion exchange methods, most will not separate Hb E and Hb A2. The delta chain can be readily separated from the beta C, beta E and beta O-Arab chains by reverse phase HPLC. Hence, reverse phase HPLC provides a means of quantitatively determining Hb A2 in the presence of Hb C, Hb E, and Hb O-Arab. The procedure, although not highly accurate, does permit the detection of increased Hb A2, for example, in beta-thal heterozygotes and, therefore, is applicable to other conditions (Hb C, Hb E, Hb O-Arab).
Schroeder
W A
WA
Shelton
J B
JB
Shelton
J R
JR
Huynh
V
V
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005914:Globins / Q000302:isolation & purification; D006441:Hemoglobin A / Q000302:isolation & purification*; D006443:Hemoglobin A2 / Q000302:isolation & purification*; D006444:Hemoglobin C / Q000302:isolation & purification*; D006446:Hemoglobin E / Q000302:isolation & purification*; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification*; D006801:Humans; D013789:Thalassemia / Q000097:blood
D006455:Hemoglobins, Abnormal; D005914:Globins; D006444:Hemoglobin C; D006441:Hemoglobin A; D006443:Hemoglobin A2; D006446:Hemoglobin E; C032031:hemoglobin Arab