Structure of the capsular polysaccharide of Haemophilus pleuropneumoniae serotype 5.
The capsular polysaccharide of Haemophilus (Actinobacillus) pleuropneumoniae serotype 5 (ATCC 33377) was found to be a linear type polysaccharide of a repeating disaccharide unit composed of 2-acetamido-2-deoxy-D-glucose and 3-deoxy-D-manno-2-octulosonic acid (dOclA). By composition analysis, methylation, partial hydrolysis and 1H and 13C nuclear magnetic resonance studies, it was concluded that the capsular polysaccharide is a high-molecular-mass unbranched polymer having the structure: [6)-alpha-D-GlcNAcp-(1-5)-beta-dOclAp-(2]n.
Division of Biological Sciences, National Research Council of Canada, Ottawa.
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Altman
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Brisson
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Perry
D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D008401:Gas Chromatography-Mass Spectrometry; D006190:Haemophilus / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D012703:Serotyping
D002241:Carbohydrates; D011135:Polysaccharides, Bacterial