Isolation and characterization of variant IGF-1 as well as IGF-2 from adult human brain.
The forms of somatomedin present in the adult human brain have been characterized in this study. Two peptides were purified by acidification, size exclusion chromatography, affinity chromatography, FPLC and HPLC. Structural analysis identified these peptides as the variant form of IGF-1 with a truncated N-terminal region earlier isolated from human fetal brain and IGF-2. The presence of the truncated IGF-1 variant and IGF-2 in the human CNS suggests their role as neuropeptides.
C
C
Carlsson-Skwirut
H
H
Jörnvall
A
A
Holmgren
C
C
Andersson
T
T
Bergman
G
G
Lundquist
B
B
Sjögren
V R
VR
Sara
D000328:Adult; D000595:Amino Acid Sequence; D001921:Brain / Q000196:embryology; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D014644:Genetic Variation; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000302:isolation & purification*; D007335:Insulin-Like Growth Factor II / Q000302:isolation & purification*; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D011869:Radioligand Assay; D013002:Somatomedins / Q000302:isolation & purification*
D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II