[The effects of serum from tumor-bearing mice on mitogen-induced blastogenesis of normal spleen cells].
In order to detect the serum immunosuppressive factors of tumor bearer, the effects of normal C3H/He mice serum (NMS), serum from methylcholanthrene induced fibrosarcoma bearing mice (F4w serum) and sera fractionated by high performance liquid chromatography (HPLC) using anion exchange column were examined on mitogen-induced blastogenesis of normal murine spleen cells (Nspc). The addition of 5% of NMS suppressed the blastogenesis of Nspc. Less than 0.6% of F4w serum suppressed the blastogenesis. NMS was applied to HPLC and was separated to four peaks (A,B,C,D). In HPLC pattern of F4w serum, peak B was higher than that of NMS and new peak (E) was observed next to peak D. Peak D of NMS suppressed the blastogenesis. Not peak D but peak E of F4w serum suppressed the blastogenesis. The properties of those blastogenesis-suppressive fractions were discussed.
Tsuchiya
K
K
Tanaka
T
T
Inaba
S
S
Nomi
S
S
Hamagashira
K
K
Hamada
H
H
Oka
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology*; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D008809:Mice, Inbred C3H; D009363:Neoplasm Proteins; D051381:Rats; D011916:Rats, Inbred F344
D006023:Glycoproteins; D037102:Lectins; D009363:Neoplasm Proteins; C038588:serum immunosuppressive factor, human; D008748:Methylcholanthrene