A dot screening procedure on nitrocellulose for detecting anti-idiotopes in hybridoma supernatants.
A dot assay on nitrocellulose was developed for screening hybridoma fusions for the presence of anti-idiotopes. The technique involves spotting of hybridoma supernatants onto nitrocellulose filters, and detection of anti-idiotope with an alkaline phosphatase-coupled idiotype.
Borden
P
P
Kabat
E A
EA
D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D003101:Collodion; D006825:Hybridomas / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D003101:Collodion