A new rapid silver impregnation for neuronal bodies.
Frozen sections of avian and rat brains routinely fixed in 6% glutaraldehyde/2% paraformaldehyde and left in phosphate buffer for 1 month are cut at 15-20 microns and collected in distilled water. Sections are placed in ammoniacal silver solution for 30 s, rinsed in 100% acetone and developed in a reducing solution at 75 degrees C. Sections are toned in 1% gold chloride solution and fixed in 50% sodium thiosulfate. After washing, the sections are dehydrated, cleared and mounted in the usual way for light microscopy.
Department of Morphology and Cellular Biology, Faculty of Biology and Medicine, University of Oviedo, Spain.
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Tolivia
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Tolivia
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D002645:Chickens; D009474:Neurons / Q000166:cytology*; D051381:Rats; D012834:Silver
C000212:ammoniacal silver; D012834:Silver