Modification of calf thymus DNA by methyl methanesulfonate. Quantitative determination of 7-methyldeoxyguanosine by mass spectrometry.
Quantitation of 7-methyldeoxyguanosine (m7dG) produced in the in vitro methyl methanesulfonate (MeMS) action on calf-thymus DNA is achieved by enzymatic degradation, liquid chromatographic separation and chemical ionization mass spectrometry. The total degree of methylation, measured by uptake of [14C]MeMS was 0.35%. Mass spectral analysis shows that m7dG constitutes 84% of the total methylated product. It is also shown that tandem mass spectrometry allows detection of m7dG, as the protonated base, down to 1 pmol level, suggesting that MS/MS analysis can be the method of choice in quantitation of the adducts of in vivo DNA modifications.
Chang
C J
CJ
Ashworth
D J
DJ
Isern-Flecha
I
I
Jiang
X Y
XY
Cooks
R G
RG
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D013058:Mass Spectrometry; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D013950:Thymus Gland / Q000378:metabolism
C048746:7-methyl-2-deoxyguanosine; D004247:DNA; D008741:Methyl Methanesulfonate; D003849:Deoxyguanosine