Studies on the succinate dehydrogenating system. Interaction of the mitochondrial succinate-ubiquinone reductase with pyridoxal phosphate.
The inhibitory effect of pyridoxal phosphate on the Triton X-100 solubilized purified bovine heart succinate-ubiquinone reductase (Choudhry, Z.M., Gavrikova, E.V., Kotlyar, A.B., Tushurashvilli, P.R. and Vinogradov, A.D. (1985) FEBS Lett. 182, 171-175) was studied. The kinetics of the enzyme inactivation by pyridoxal phosphate was found to be strongly dependent both qualitatively and quantitatively on the concentration of the protein-detergent complexes. In the diluted system the inactivation of the ubiquinone-depleted enzyme was completely prevented by the saturating concentrations of Q2, carboxin, thenoiltrifluoroacetone and pentachlorophenol, i.e., by the substrate and specific inhibitors of the enzyme. The protective effects of Q2 and the inhibitors was employed to quantitate the affinities of the ligands to their specific binding sites. Strong difference in the affinity of Q2 to the reduced and oxidized enzyme was found. When the soluble reconstitutively active succinate dehydrogenase was treated with pyridoxal phosphate, the reactivity of the enzyme towards low ferricyanide concentrations and its reconstitutive activity was significantly protected against aerobic inactivation.
Choudhry
Z M
ZM
Kotlyar
A B
AB
Vinogradov
A D
AD
D000818:Animals; D001665:Binding Sites; D002261:Carboxin / Q000494:pharmacology; D002417:Cattle; D042963:Electron Transport Complex II; D005033:Ethylmaleimide / Q000494:pharmacology; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010416:Pentachlorophenol / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid; D014451:Ubiquinone / Q000378:metabolism / Q000494:pharmacology
D009097:Multienzyme Complexes; D013386:Succinates; D014451:Ubiquinone; D002261:Carboxin; D011732:Pyridoxal Phosphate; D019802:Succinic Acid; D010416:Pentachlorophenol; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; C025204:Ubiquinone Q2; D005033:Ethylmaleimide