Phosphorylation of class I HLA antigens in U-937 monocyte-like cells. Role of protein kinase C.
Phorbol esters and 1-oleoyl-2-acetylglycerol induce an increase in phosphorylation of a series of 44-kD proteins in the U-937 human monocyte-like cell line. The phenomenon is rapid at 37 degrees C, starting 0.5 min after adding the inducer, increasing to a maximum of 5-10 min and decreasing to the basal level at 30 min. It is also observed at 4 degrees C, though much more slowly. Immunoprecipitation and two-dimensional electrophoresis demonstrated that these phosphorylated proteins belong to the class I HLA antigens.
Rouis
M
M
Thomopoulos
P
P
Haziot
A
A
Broquet
C
C
D002460:Cell Line; D004075:Diglycerides / Q000494:pharmacology; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D013696:Temperature
D004075:Diglycerides; D006680:HLA Antigens; D010703:Phorbol Esters; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C