L-methionine enhances the contractile responses of rat uterine smooth muscle to acetylcholine and high KCl.
The contractile responses of isolated uterine smooth muscle from estradiol-treated ovariectomized rats to acetylcholine and high KCl in Ca-depleted modified Locke-Ringer (without CaCl2 and with 0.1 mM EGTA) solution on addition of CaCl2 (final concentration, 5 mM) are used as an indicator of Ca influx through the Ca channel. L-Methionine significantly enhanced these responses in the absence and presence of 3-deazaadenosine plus homocysteine thiolactone, blockers of methylation. These findings suggest that methylations of some components in isolated uterine smooth muscle seem to be important in stimulation-contraction coupling in the muscle.
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D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D005260:Female; D006710:Homocysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D008715:Methionine / Q000494:pharmacology*; D008745:Methylation; D010743:Phospholipids / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014372:Tubercidin / Q000494:pharmacology; D014590:Uterine Contraction / Q000187:drug effects*
D010743:Phospholipids; D011506:Proteins; C018258:3-deazaadenosine; D006710:Homocysteine; D011189:Potassium Chloride; D008715:Methionine; C007957:homocysteine thiolactone; D014372:Tubercidin; D002122:Calcium Chloride; D000109:Acetylcholine