In vitro absorption of some o-phthalate diesters through human and rat skin.
The absorption of undiluted phthalate diesters [dimethyl phthalate (DMP), diethylphthalate (DEP), dibutyl phthalate (DBP) and di-(2-ethylhexyl)phthalate (DEHP)] has been measured in vitro through human and rat epidermal membranes. Epidermal membranes were set up in glass diffusion cells and their permeability to tritiated water measured to establish the integrity of the skin before the phthalate esters were applied to the epidermal surface. Absorption rates for each phthalate ester were determined and a second tritiated water permeability assessment made to quantify any irreversible alterations in barrier function due to contact with the esters. Rat skin was consistently more permeable to phthalate esters than the human skin. As the esters became more lipophilic and less hydrophilic, the rate of absorption was reduced. Contact with the esters caused little change in the barrier properties of human skin, but caused marked increases in the permeability to water of rat skin. Although differences were noted between species, the absolute rates of absorption measured indicate that the phthalate esters are slowly absorbed through both human and rat skin.
Central Toxicology Laboratory, Imperial Chemical Industries PLC, Cheshire, UK.
R C
RC
Scott
P H
PH
Dugard
J D
JD
Ramsey
C
C
Rhodes
D000042:Absorption; D000818:Animals; D003993:Dibutyl Phthalate / Q000493:pharmacokinetics; D004051:Diethylhexyl Phthalate / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010795:Phthalic Acids / Q000493:pharmacokinetics*; D051381:Rats; D012867:Skin / Q000378:metabolism*
D010795:Phthalic Acids; C024629:dimethyl phthalate; D003993:Dibutyl Phthalate; D004051:Diethylhexyl Phthalate; C007379:diethyl phthalate