Expression of the egg-laying hormone gene family in the head ganglia of Aplysia.
RNA blotting and cDNA cloning techniques were used to study expression of the egg-laying hormone (ELH) gene family in the head ganglia of Aplysia californica. All head ganglia were found to express a 1800 nucleotide (nt) mRNA homologous to the ELH gene family. The nucleotide sequence of a clone isolated from a ring ganglia cDNA library demonstrates that this message encodes the ELH precursor. Further studies demonstrate the presence of a smaller, 1500 nt, transcript which encodes the peptide A precursor. However, the level of this mRNA is at least 10-fold lower than the ELH encoding message.
Shyamala
M
M
Nambu
J R
JR
Scheller
R H
RH
D000818:Animals; D001048:Aplysia / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004274:DNA, Recombinant; D005724:Ganglia / Q000378:metabolism*; D007447:Invertebrate Hormones / Q000096:biosynthesis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*
D004274:DNA, Recombinant; D007447:Invertebrate Hormones; D012333:RNA, Messenger; C026880:egg-laying hormone, Mollusca; D004247:DNA