Liver trophism in dogs made diabetic by total pancreatectomy or alloxan administration.
1. In order to evaluate hepatic trophism in diabetic dogs, an experimental study was carried out on 30 adult mongrel dogs of both sexes weighing 11.9 +/- 1.6 kg, divided into three groups: 1) Controls (C) (N = 10), submitted to partial (30%) hepatectomy; 2) PD Animals (N = 10), submitted to partial hepatectomy plus total pancreatectomy with preservation of the duodenum during the same surgical intervention; 3) and AD Animals (N = 10), submitted to partial and simultaneously made diabetic with alloxan. 2. The animals were submitted to an oral glucose tolerance test (OGTT) one day before and on the 7th day after partial hepatectomy to evaluate the severity of diabetes. During the post-hepatectomy period the fasting glycemia values were similar for both diabetic groups (greater than 200 mg%). During OGTT, blood glucose levels of the diabetic groups peaked at 60 min but were significantly higher for the AD than for the PD group. The difference persisted at 120 and 180 min, but was no longer statistically significant. 3. Liver trophism was evaluated by measuring liver RNA content and the nuclear volume of hepatocytes. Both diabetic groups showed significantly lower RNA contents and absence of nuclear hypertrophy compared to partially hepatectomized controls probably because of the severe diabetes induced in these animals.
Departamento de Cirurgia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
Castro-e-Silva O Júnior
R
R
Ceneviva
A L
AL
Ferreira
M C
MC
Foss
F L
FL
De-Lucca
D000496:Alloxan; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism / Q000503:physiopathology*; D008115:Liver Regeneration; D008297:Male; D010180:Pancreatectomy
D001786:Blood Glucose; D000496:Alloxan