Vascular reorganization and return of rigidity in fracture healing.
The present study addresses the relationship between vascular reorganization and the biomechanical changes occurring during canine long bone fracture healing. A middiaphyseal osteotomy of the radius was performed, and angiographic studies were carried out using India ink injections from 2 to 12 weeks postosteotomy. The radii were harvested, subjected to nondestructive four-point bend testing on an Instron, and then sectioned and cleared according to the Spaltholtz technique. The observed microangiographic patterns were correlated with the calculated rigidity values. Quantitative bone blood flow measurements around the osteotomy were performed on a different group of animals, using the radiotracer microsphere technique, at 3, 6, and 9 weeks postosteotomy to correlate with our qualitative microangiographic results. We demonstrated a direct and predictable relationship between the phase of vascular reorganization and the rigidity. This correlation was present even in our delayed union specimens.
Chidgey
L
L
Chakkalakal
D
D
Blotcky
A
A
Connolly
J F
JF
D000792:Angiography / Q000379:methods; D000818:Animals; D001696:Biomechanical Phenomena; D001783:Blood Flow Velocity; D002244:Carbon; D004396:Coloring Agents; D004285:Dogs; D008833:Microcirculation / Q000000981:diagnostic imaging; D008863:Microspheres; D010027:Osteotomy; D011884:Radius / Q000098:blood supply*; D011885:Radius Fractures / Q000503:physiopathology*; D013718:Tensile Strength; D014945:Wound Healing
D004396:Coloring Agents; C028433:chinese ink; D002244:Carbon