A new sialoglycoprotein from rat seminal vesicle and its association with semen coagulum.
By radiolabeling using NaIO4/[3H]KBH4, a new sialoglycoprotein with Mr of 19,000 was found in the secretion of rat seminal vesicle. It was shown to interact non-covalently with semen coagulum. It existed in three acidic forms with pI values of 4.1, 3.7 and 2.9 and possessed high contents of sialic acids and acidic amino acids.
T
T
Limpaseni
M
M
Chulavatnatol
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001894:Borohydrides; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D007452:Iodates; D007526:Isoelectric Point; D007553:Isotope Labeling; D008297:Male; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D012661:Semen / Q000032:analysis / Q000502:physiology*; D012669:Seminal Vesicles / Q000378:metabolism*; D012794:Sialic Acids / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis / Q000378:metabolism*; D014316:Tritium
D000596:Amino Acids; D001894:Borohydrides; D007452:Iodates; D012794:Sialic Acids; D012795:Sialoglycoproteins; C028371:potassium borohydride; D014316:Tritium; D019158:N-Acetylneuraminic Acid; C032285:sodium iodate