[Regulators of 3-hydroxybutyrate dehydrogenase activity in rat liver mitochondria].
Effects of numerous organic acids on the 3-hydroxybutyrate dehydrogenase activity were studied in isolated rat liver mitochondria with nonspecific permeability. Amino acids, most of citric acid cycle intermediates, lactate, maleate, acetate, glycerol-3-phosphate, urea, palmitate, and phosphoenolpyruvate plus ADP were shown to modify the enzyme activity insignificantly. The inhibitory effect of pyruvate seems to be a result of the concomitant cytosolic lactate dehydrogenase activity, and the effect of oxaloacetate is that of the mitochondrial matrix malate dehydrogenase activity. Malonate proves to be a competitive inhibitor of the 3-hydroxybutyrate dehydrogenase activity, enzyme affinity for malonate being the same irrespective of the source or purification of the preparation.
Kosenko
E A
EA
Kaminskiĭ
Iu G
IuG
D020155:3-Hydroxybutyric Acid; D000818:Animals; D002463:Cell Membrane Permeability; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006884:Hydroxybutyrate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity
D006885:Hydroxybutyrates; D006884:Hydroxybutyrate Dehydrogenase; D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase; D009245:NADH Dehydrogenase; D020155:3-Hydroxybutyric Acid
Reguliatory aktivnosti 3-oksibutiratdegidrogenazy v mitokhondriiakh pecheni krysy.