The in vitro cytotoxicity of asbestos fibers: I. P388D1 cells.
In this study, the cytotoxicity of 13 fibrous samples of known fiber number and dimensions has been established in P388D1 cells. The cells were exposed in vitro to dust concentrations of 10 or 50 micrograms/ml and, after incubation for 24 or 48 hours, any changes in cellular viability, lactate dehydrogenase, and glucosaminidase levels were determined. In general, there was a close association between the reduction in cellular viability and the loss of intracellular enzymes induced by each dust, the chrysotile asbestos samples proving more cytotoxic than the amphiboles. The cytotoxicity of the fibrous dusts was shown to be related to the number of fibers greater than 8 micron in length in the samples.
A
A
Wright
H
H
Cowie
I P
IP
Gormley
J M
JM
Davis
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D006596:Hexosaminidases / Q000378:metabolism; D007941:Leukemia P388 / Q000201:enzymology / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D013329:Structure-Activity Relationship
D001194:Asbestos; D006596:Hexosaminidases