Ascorbate in plasma as measured by liquid chromatography and by dichlorophenolindophenol colorimetry.
Ascorbic acid was measured in 125 plasma samples by an automated colorimetric method involving dichlorophenolindophenol and by a "high-performance" liquid-chromatographic procedure with electrochemical detection. The two methods gave comparable results for samples with ascorbate concentrations of 1 to 20 mg/L (r = 0.97). We also measured the amount of total ascorbate (ascorbic acid + dehydroascorbic acid) in the same samples by a liquid-chromatographic procedure with precolumn derivitization of ascorbic acid. We confirmed that plasma contains little dehydroascorbic acid.
VanderJagt
D J
DJ
Garry
P J
PJ
Hunt
W C
WC
D015086:2,6-Dichloroindophenol; D001205:Ascorbic Acid / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D006801:Humans; D007215:Indophenol / Q000031:analogs & derivatives*
D007215:Indophenol; D015086:2,6-Dichloroindophenol; D001205:Ascorbic Acid