Fluorescence immunohistochemical detection of hypoxic cells in spheroids and tumours.
Polyclonal antibodies have been raised in rabbits to a haemocyanin adduct of a reductively-activated, fluorinated analogue of misonidazole. Fluorescence immunohistochemical studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in patterns similar to those revealed by autoradiographic studies with a tritium-labelled derivative of the fluorinated misonidazole analogue.
Radiobiology, Cross Cancer Institute, Edmonton, Alberta, Canada.
J A
JA
Raleigh
G G
GG
Miller
A J
AJ
Franko
C J
CJ
Koch
A F
AF
Fuciarelli
D A
DA
Kelly
D000818:Animals; D002279:Carcinoma 256, Walker / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008856:Microscopy, Fluorescence; D008920:Misonidazole / Q000378:metabolism; D009593:Nitroimidazoles / Q000378:metabolism*; D010100:Oxygen; D011817:Rabbits; D014407:Tumor Cells, Cultured; D005466:Fluorocarbons
D008920:Misonidazole; D009593:Nitroimidazoles; D010100:Oxygen; C049948:CCI 103F; D005466:Fluorocarbons