Pyridine nucleotides and redox state regulation of aflatoxin biosynthesis in Aspergillus parasiticus NRRL 3240.
In vivo regulation of lipid and aflatoxin biosynthesis by pyridine nucleotides and their derived functions was studied in Aspergillus parasiticus NRRL 3240. Aflatoxins, total lipids and pyridine nucleotide content were estimated under different growth conditions. Aflatoxin formation was highest in cultures grown in sucrose-low salts medium followed by asparagine- and zinc-deficient media. The lipid content of the cultures followed an inverse pattern. The levels of oxidized nucleotides decreased with age under all culture conditions employed. Concentrations of NADPH peaked before the onset of aflatoxin biosynthesis. For each medium used, the estimated catabolite reduction charge was constant at all stages of growth whereas the anabolic reduction charge varied. A direct relationship between the level of extracellular ammonium ions and anabolic reduction charge was established. A high anabolic reduction charge was associated with increased lipid biosynthesis rather than aflatoxin biosynthesis.
R K
RK
Bhatnagar
S
S
Ahmad
K G
KG
Mukerji
T A
TA
Subramanian
D000348:Aflatoxins / Q000096:biosynthesis*; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D008055:Lipids / Q000096:biosynthesis; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds
D000348:Aflatoxins; D003470:Culture Media; D008055:Lipids; D000644:Quaternary Ammonium Compounds; D009243:NAD; D009249:NADP