Enhanced isolation of respiratory syncytial virus in cell culture.
During two winter seasons, we found that the combination of WI-38 or MRC-5 human lung fibroblasts plus primary rhesus monkey kidney (RhMK) and HEp-2 cell cultures yielded maximal isolation of respiratory syncytial virus. Cytopathic effects (CPE) developed earliest in RhMK cells and slowest in the human fibroblast lines. In RhMK cells, 50% of ultimately positive cultures showed CPE in 5 days, and 90% of positive cultures showed CPE within 7 days during both respiratory syncytial virus seasons.
Arens
M Q
MQ
Swierkosz
E M
EM
Schmidt
R R
RR
Armstrong
T
T
Rivetna
K A
KA
D000818:Animals; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D007223:Infant; D008253:Macaca mulatta; D009305:Nasopharynx / Q000382:microbiology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology; D010253:Respirovirus Infections / Q000175:diagnosis* / Q000382:microbiology
D003470:Culture Media