Subfornical organ: effects of salt loading and water deprivation on in vitro radioamino acid incorporation into individual proteins.
The subfornical organ of the brain has a role in the regulation of fluid balance in higher animals. In this study the effects of salt loading and water deprivation on specific proteins in this organ were investigated. For 4 days, 3 groups of rats were given an appropriate fluid diet (control, 2% NaCl and water deprived), with all groups having free access to food. Animals were killed by decapitation, and the subfornical organ was quickly dissected out and incubated for 6 h in a medium containing [35S]methionine and [35S]cysteine. Proteins from these organs were then separated by two-dimensional electrophoresis, and the resulting autofluorographs were analyzed by scanning densitometry. The results show that the incorporation of labeled amino acids into 8 proteins was changed due to the experimental manipulations.
D M
DM
Santer
W E
WE
Heydorn
G J
GJ
Creed
D C
DC
Klein
D M
DM
Jacobowitz
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D003545:Cysteine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008297:Male; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology*; D013356:Subfornical Organ / Q000187:drug effects / Q000378:metabolism*; D014868:Water Deprivation / Q000502:physiology*
D009419:Nerve Tissue Proteins; D012965:Sodium Chloride; D008715:Methionine; D003545:Cysteine