Possible relationship between pervasive developmental disorders and platelet monoamine oxidase activity.
1. The present study was undertaken to determine if the platelet monoamine oxidase (MAO) activity of children with childhood-onset Pervasive Developmental Disorders (PDD), atypical PDD and autistic children differs from MAO of normal children of the same age. 2. The kinetic parameters of MAO activity (Km and Vmax for kynuramine as substrate in 100 mM sodium phosphate buffer, pH 7.4 at 37 degrees C) were determined for platelets from autistic (N = 6), childhood onset PDD (N = 6) and atypical PDD (N = 6) children and 14 controls aged 6-10 years. 3. PDD children had significantly lower Km (4.41 +/- 0.26 vs 5.30 +/- 0.23 microM) and Vmax (16.77 +/- 1.56 vs 22.15 +/- 2.16 nmol h-1 mg protein-1) than control children. The reduction in Vmax was demonstrable in MAO activity measured with 100 microM substrate (14.93 +/- 1.13 vs 20.96 +/- 2.10 nmol h-1 mg-1). 4. These data show that childhood-onset PDD patients, in which the syndrome was complete, presented the lowest levels of platelet MAO activity.
Cátedra de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
E J
EJ
Filinger
M A
MA
García-Cotto
S
S
Vila
H
H
Gerbaldo
D
D
Jerez
D001321:Autistic Disorder / Q000097:blood*; D001792:Blood Platelets / Q000201:enzymology*; D002648:Child; D002659:Child Development Disorders, Pervasive / Q000097:blood*; D006801:Humans; D007700:Kinetics; D007735:Kynuramine / Q000097:blood*; D008995:Monoamine Oxidase / Q000097:blood*; D011427:Propiophenones / Q000097:blood*
D011427:Propiophenones; D007735:Kynuramine; D008995:Monoamine Oxidase