Kinetic description of proteolysis. Part 4. Hydrolysis kinetics of partial protein hydrolysates.
This report presents experimental data on kinetics of casein hydrolysate hydrolysis. The experiments were interpreted in the framework of a theory reported, which is guided by an experimentally measurable value--a total molar concentration of all peptides and amino acids (total amino nitrogen). The total amino nitrogen N was determined by photometry of N-trinitrophenyl (N-TNP) derivatives obtained by means of trinitrobenzenesulfonic acid (TNBS). Unlike the procedures reported previously, we stopped the proteolysis reaction by boiling, and performed the trinitrophenylation in a 20% V. aqueous solution of MeCN.
Vorob'ev
M M
MM
A. N. Nesmeyanov Institute of Organo-Element Compounds, USSR Academy of Science, Moscow.
Slobodyanikova
L S
LS
Vitt
S V
SV
Latov
V K
VK
Belikov
V M
VM
D000588:Amines / Q000032:analysis; D002364:Caseins / Q000032:analysis; D002918:Chymotrypsin / Q000378:metabolism; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D009584:Nitrogen / Q000032:analysis; D010783:Photometry; D011492:Protein Hydrolysates / Q000032:analysis / Q000378:metabolism*
D000588:Amines; D002364:Caseins; D007202:Indicators and Reagents; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D002918:Chymotrypsin; D009584:Nitrogen