Purification of phospholipase D from citrus callus tissue.
Phospholipase D in extracts of soluble proteins from callus cultures derived from cotyledons of Citrus sinensis (L.) Osbeck is activated by Ca2+ and anionic detergents and has a pH optimum of 6.5. The enzyme was purified 703-fold over the crude protein extract with a yield of 15% by ammonium sulfate precipitation, ion exchange chromatography, gel filtration, hydrophobic interaction chromatography, and preparative acrylamide gel electrophoresis. Preparative electrophoresis was carried out using conventional slab gel equipment and electroelution of the sliced gel. Analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified phospholipase revealed two bands of the same staining intensity running at 94.2K and 90.5K.
Fruit Crops Department, University of Florida, Gainesville 32611.
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Mayer
D002122:Calcium Chloride / Q000494:pharmacology; D002478:Cells, Cultured; D002957:Citrus; D007700:Kinetics; D008970:Molecular Weight; D010739:Phospholipase D / Q000302:isolation & purification* / Q000378:metabolism; D010740:Phospholipases / Q000302:isolation & purification*; D010944:Plants / Q000201:enzymology*
D010740:Phospholipases; D010739:Phospholipase D; D002122:Calcium Chloride