Chick oviduct progesterone receptor phosphorylation: characterization of a copurified kinase and phosphorylation in primary cultures.
A protein kinase activity was copurified with the chick oviduct progesterone receptor. The enzyme is magnesium dependent and can use the B subunit of progesterone receptor or histones as substrates. The physiochemical parameters of the kinase were determined [pI approximately 5.3; Stokes radius approximately 7.2 nm; sedimentation coefficient (S 20,w) approximately 5.6] and compared to those of the purified B subunit. The results were consistent with the presence of an unique enzyme distinct from the receptor itself. The physiological significance of receptor phosphorylation was investigated in oviduct cells grown in primary culture. Cells were labeled with [32P]orthophosphate in presence or absence of progesterone and the receptor components were immunoprecipitated with a specific polyclonal antibody. Although progesterone treatment lead to the attachment of most of the receptor (approximately 80%) to nuclear structures, the 32P-labeled B subunit was only recovered in the cytosol fraction. Different procedures to extract the nuclear receptor did not allow detection of any 32P-labeled form in the nuclear-soluble fractions, suggesting that the B subunit was not further phosphorylated upon the exposure of cells to progesterone.
Garcia
T
T
Inserm U33. Lab. Hormones, Bicêtre, France.
Buchou
T
T
Jung-Testas
I
I
Renoir
J M
JM
Baulieu
E E
EE
D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005260:Female; D006657:Histones / Q000378:metabolism; D010057:Oviducts / Q000201:enzymology; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification*; D011980:Receptors, Progesterone / Q000302:isolation & purification / Q000378:metabolism*
D006657:Histones; D011980:Receptors, Progesterone; D011494:Protein Kinases