Enterohepatic circulation of N-acetyl-leukotriene E4.
N-Acetyl-leukotriene E4, the end product of leukotriene C4 metabolism in the mercapturic acid pathway, was rapidly eliminated from the blood circulation into the bile of rats. Part of the N-acetyl-leukotriene E4 secreted from bile into the intestine underwent enterohepatic circulation. Leukotriene absorption occurred from the small intestine and from the colon. Biliary and urinary excretion within 5.5 h amounted to 15 and 2%, respectively, of the intraduodenally administered N-acetyl- 3H leukotriene E4 in animals anesthetized with ketamine. HPLC analyses indicated that 35% of the biliary radioactivity corresponded to unchanged N-acetyl-3H leukotriene E4, while 65% in bile and 100% in urine were polar metabolites. Enterohepatic circulation extends the biological half-life of N-acetyl-leukotriene E4.
Biochemisches Institut, Universität Freiburg, F.R.G.
A
A
Guhlmann
W
W
Hagmann
D
D
Keppler
D000818:Animals; D001646:Bile / Q000378:metabolism; D004764:Enterohepatic Circulation; D005260:Female; D007267:Injections; D007275:Injections, Intravenous; D007408:Intestinal Absorption; D007422:Intestines; D017999:Leukotriene E4 / Q000031:analogs & derivatives*; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism
D013189:SRS-A; D017999:Leukotriene E4; C047630:N-acetylleukotriene E4