Indirect spectrophotometric method for determining epicillin.
A new UV spectrophotometric assay for determination of epicillin in capsules and plasma is reported. The method is based on the absorptivity of a degradation product obtained from the acidic hydrolysis of the antibiotic. The isolation, identification, individual capsule assays, composite assay, and recovery studies are described. Also, the proposed method is compared with a dc polarographic assay in plasma.
Núñez-Vergara
L J
LJ
Roa
A
A
Squella
J A
JA
González-Barbagelata
R V
RV
D000667:Ampicillin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D002214:Capsules / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D011048:Polarography; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D002214:Capsules; C100212:epicillin; D000667:Ampicillin