Structure of asparagine-linked sugar chains in the major polypeptide of hepatitis B surface antigen.
Asparagine-linked sugar chains were quantitatively released from hepatitis B surface antigen on hydrazinolysis followed by N-acetylation and NaB3H4 reduction. The released oligosaccharides were composed of two major sialylated components and a trace of a neutral component. From the results of the combination of sequential exoglycosidase digestion and methylation analysis, the structures of the acidic and the neutral components were deduced to be NeuAc alpha 2----6Gal beta 1----4GlcNAc beta 1----2Man alpha 1----3(+/- NeuAc alpha 2----6Gal beta 1----4GlcNAc beta 1----2Man alpha 1----6)Man beta 1----4GlcNAc beta 1----4GlcNAcOT and Gal beta 1----4GlcNAc beta 1----2Man alpha 1----3(Gal beta 1----4GlcNAc beta 1----2Man alpha 1----6)Man beta 1----4GlcNAc beta 1----4GlcNAcOT, respectively.
K
K
Hanaoka
S
S
Takasaki
A
A
Kobata
H
H
Miyamoto
T
T
Nakamura
M
M
Mayumi
D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral; D001216:Asparagine / Q000502:physiology*; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D004590:Electrophoresis, Paper; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D006834:Hydrazines / Q000494:pharmacology; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis*; D010455:Peptides / Q000032:analysis*
D000954:Antigens, Surface; D000956:Antigens, Viral; D006514:Hepatitis B Surface Antigens; D006834:Hydrazines; D009844:Oligosaccharides; D010455:Peptides; C029424:hydrazine; D001216:Asparagine; D006026:Glycoside Hydrolases