Cell-free biosynthesis of arphamenine A.
Arphamenine A was synthesized in a cell-free system obtained from the arphamenine-producing strain, Chromobacterium violaceum BMG361-CF4. L-[14C]-phenylalanine was converted to beta-phenylpyruvic acid by phenylalanine amino-transferase obtained from the 10,000 x g supernatant (S10 fraction). [14C]-Benzylmalic acid was synthesized from beta-phenylpyruvic acid with [14C]-acetyl-CoA in the S10 fraction. [14C]-Benzylsuccinic acid was formed from beta-phenylpyruvic acid with [14C]-acetyl-CoA and ATP in this fraction, as was [14C]-arphamenine A from benzylsuccinic acid and L-[14C]-arginine. Thus, the pathway of arphamenine A biosynthesis was confirmed by the cell-free biosynthesis of this antibiotic.
A
A
Okuyama
S
S
Ohuchi
T
T
Tanaka
T
T
Aoyagi
H
H
Umezawa
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000626:Aminopeptidases / Q000037:antagonists & inhibitors; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002861:Chromobacterium / Q000378:metabolism*; D006146:Guanidines / Q000096:biosynthesis*; D010667:Phenylpyruvic Acids / Q000378:metabolism; D013386:Succinates / Q000378:metabolism
D002250:Carbon Radioisotopes; D006146:Guanidines; D010667:Phenylpyruvic Acids; D013386:Succinates; D000105:Acetyl Coenzyme A; C040038:arphamenine A; C000249:benzylsuccinic acid; D000626:Aminopeptidases; C031606:phenylpyruvic acid