Improved methods of direct and cultured chromosome preparations from chorionic villous samples.
A new method is described for preparing direct mitotic chromosome spreads from chorionic villous samples, which has resulted in sufficiently high yields of well-banded metaphases to permit a complete standard chromosomal diagnosis in 20 of 20 cases. A method of establishing monolayer cultures from this material that can be harvested from 3 to 7 days after initiation is also presented.
M T
MT
Yu
C Y
CY
Yu
C X
CX
Yu
J
J
Maidman
D
D
Warburton
D002478:Cells, Cultured; D002824:Chorionic Villi / Q000648:ultrastructure*; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D007621:Karyotyping; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*