Quantitative gas chromatographic measurement of glycosaminoglycan hexosamines in urine and plasma.
A method is described for the quantitative determination of urine and plasma glycosaminoglycans (GAGs) by gas chromatography of the acetylated amino sugars. GAGs were first recovered by precipitation from urine with alkyltrimethylammonium bromide and from plasma by mini-column chromatography after papain digestion. Urine samples (24) analysed for total hexosamines by gas chromatography and for uronic acid by colorimetry had a correlation coefficient of 0.85. The within-run coefficient of variation (C.V.) for nineteen samples from a pooled urine was 5.2% for total hexosamines and that for the ratio of galactosamine to total hexosamines was 3.7%. The corresponding C.V. values for twelve plasma samples from a common pool were 6.5 and 3.7%. The mean ratio of galactosamine to total hexosamine in ten pre-breakfast spot urines was 51.5%. The corresponding ratio in the plasma from twenty adolescent blood donors was 76.3% and the mean total hexosamine content of the GAGs was 47.36 mumol/l.
Larking
P W
PW
Department of Pathology Services, Dunedin Public Hospital, New Zealand.
D000328:Adult; D002648:Child; D002849:Chromatography, Gas; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis* / Q000097:blood / Q000652:urine; D006595:Hexosamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D006868:Hydrolysis; D008297:Male
D006025:Glycosaminoglycans; D006595:Hexosamines