Fast and high-yielding procedures for the isolation of bovine seminal RNAase.
Fast and high yielding procedures for the isolation of bovine seminal RNAase are described. Homogeneous enzyme is prepared from seminal plasma in high yields in a single chromatographic step. Higher amounts (hundreds of mg) are easily prepared from seminal vesicles, a more available source of enzyme. Both procedures can be used also for the direct isolation of the isoenzymes of bovine seminal RNAase. An ultrarapid (1 hour) procedure is described for the preparation of mg amounts of pure enzyme, or of the individual isoenzymes, from seminal plasma.
M
M
Tamburrini
R
R
Piccoli
R
R
De Prisco
A
A
Di Donato
G
G
D'Alessio
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D007527:Isoenzymes / Q000302:isolation & purification*; D008297:Male; D012260:Ribonucleases / Q000302:isolation & purification*; D012661:Semen / Q000201:enzymology*; D012669:Seminal Vesicles / Q000201:enzymology; D013997:Time Factors
D007527:Isoenzymes; D012260:Ribonucleases