Peroxisomal oxidation of lignoceric acid in rat brain.
The oxidation of [1-14C]lignoceric acid was studied in different subcellular fractions of rat brain. The highest specific activity for oxidation of [1-14C]lignoceric acid to acetate was observed in the light mitochondrial fraction. The oxidation of [1-14C]lignoceric acid had an absolute requirement for CoASH and ATP. It was stimulated by NAD and FAD by 400 and 280 percent, respectively, whereas addition of carnitine and KCN had no effect. These properties suggest that in brain [1-14C]lignoceric acid is oxidized in peroxisomes.
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D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D002331:Carnitine / Q000494:pharmacology; D003065:Coenzyme A / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008830:Microbodies / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D010169:Palmitic Acids / Q000378:metabolism; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D005227:Fatty Acids; D010169:Palmitic Acids; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D009249:NADP; D000255:Adenosine Triphosphate; D011190:Potassium Cyanide; C010210:lignoceric acid; D002331:Carnitine; D003065:Coenzyme A