Effects of bendazac L-lysine salt on X-ray-induced cataract in the rabbit lens.
The effects of bendazac-L-lysine salt (bendazac-lysine) on some biochemical parameters (soluble and insoluble proteins, reduced glutathione, sulphydryl and disulphide groups, water content) in rabbit lens at different times after X-rays (2000 rads) were studied. The mature cataract (swelling and total lens opacity) developed 11-12 weeks after irradiation. In the mature cataract, the irradiated lenses not treated with bendazac-lysine (ILNTB) show a 32% increase in water content compared with controls; this increase is 12% in irradiated lens treated with bendazac-lysine (ILTB). Twelve weeks after irradiation the concentration of insoluble proteins in the controls, ILNTB and ILTB is 7.6%, 52.3% and 18.3% respectively. After 6, 8 and 12 weeks the concentration of reduced glutathione in ILNTB decreases by 23%, 81% and 92% as compared with the controls. In the ILTB the decrease is present only 8 and 12 weeks after X-irradiation and is of 55% and 69%, respectively. The sulphydryl-group content in the soluble proteins in ILNTB compared with the controls decreases by 26%, 38% and 47% after 6, 8 and 12 weeks, while in the ILTB a decrease is observed only after 8 and 12 weeks and is 6% and 12%, respectively. The decrease of the sulphydryl groups parallels the increase of the disulphide groups. This increase is already significant (P less than 0.01) after 6 weeks in the ILNTB, whereas it becomes significant in the ILTB only after 8 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)
L
L
Pandolfo
M A
MA
Livrea
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A
Bono
D000818:Animals; D002386:Cataract / Q000209:etiology / Q000378:metabolism*; D002850:Chromatography, Gel; D003459:Crystallins / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005978:Glutathione / Q000378:metabolism; D007191:Indazoles / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D008970:Molecular Weight; D009929:Organ Size; D011720:Pyrazoles / Q000494:pharmacology*; D011817:Rabbits; D013997:Time Factors; D014867:Water / Q000378:metabolism
D003459:Crystallins; D007191:Indazoles; D011720:Pyrazoles; D014867:Water; C036067:bendazac lysine; D005978:Glutathione; D004229:Dithiothreitol