title: Prometaphase chromosome analysis as a routine diagnostic technique.
abstract: The use of techniques for the production of prometaphase chromosomes leading to increased resolution of banding patterns has resulted in problems as well as advantages in their routine use. A simple technique utilising the folate antagonist methotrexate has been in use in this regional diagnostic cytogenetics centre for three years, and a number of chromosome abnormalities have been detected in cases previously reported as normal following G banding studies. With reference to this, the routine analysis of prometaphase-type chromosomes is discussed and assessed.
mesh_terms: D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D006801:Humans; D008677:Metaphase; D008727:Methotrexate
chemical_list: D008727:Methotrexate