Phosvitin isolation from fish eggs: methodological improvements including 'specific' phosvitin precipitation with ferric ion.
Modifications of the method of Wallace et al. [Can. J. Biochem. 44, 1647-1655 (1966)] for phosvitin isolation from vertebrate eggs were devised to enhance the method's general effectiveness. Phosvitins which do not precipitate on dilution from solutions of their lipovitellin complexes may be selectively adsorbed onto, and desorbed from, DEAE-cellulose. Phosvitins which are too small for dialysis or ultrafiltration may be concentrated or desalted by precipitation with stoichiometric amounts of ferric ions, followed by iron removal with EDTA on gel filtration. Since phosvitin distribution among ammonium sulfate fractions depends on initial protein and salt concentrations in a species-specific manner, pilot experiments are needed to establish conditions for optimal fractionation.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D011232:Chemical Precipitation; D004527:Egg Proteins / Q000302:isolation & purification*; D015387:Egg Proteins, Dietary; D007501:Iron; D046911:Macromolecular Substances; D010063:Ovum / Q000032:analysis*; D010774:Phosvitin / Q000302:isolation & purification*; D014337:Trout
D000596:Amino Acids; D004527:Egg Proteins; D015387:Egg Proteins, Dietary; D046911:Macromolecular Substances; D010774:Phosvitin; C001549:lipovitellin; D007501:Iron