Responsiveness of mouse calvaria to parathyroid hormone after explant cryopreservation: 45Ca release in vitro.
Newborn mouse calvaria prelabeled with 45Ca and cryopreserved at -196 degrees C in serum-free medium containing dimethylsulfoxide were compared to unpreserved explants for response to parathyroid hormone during subsequent culture. After short-term cryopreservation followed by rapid thawing, the viable explants continued to release 45Ca to the culture medium but additions of parathyroid hormone to the medium did not cause increased bone resorption. The data suggest that cryopreservation and thawing impairs mechanisms responsible for parathyroid hormone action on bone cells.
D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D005615:Freezing; D006801:Humans; D007700:Kinetics; D051379:Mice; D010281:Parathyroid Hormone / Q000494:pharmacology*; D014021:Tissue Preservation
D002132:Calcium Radioisotopes; D010281:Parathyroid Hormone; D002118:Calcium