Anti-leukotriene D4 action of a new anti-asthmatic drug (KC-404) on the guinea-pig isolated trachea.
Anti-asthmatic drug, KC-404, inhibited specifically spontaneous contraction and leukotriene (LT) D4-induced contraction of guinea-pig trachea. Relaxation of spontaneous contraction by KC-404 was reduced by the treatment of the guinea-pig trachea with a cyclooxygenase inhibitor, flurbiprofen. Some parallel shift of the concentration-action curve of LTD4 by KC-404 was still observed in the presence of flurbiprofen. Specific binding of [3H]LTD4 to the microsomal fraction of guinea-pig trachea was partially inhibited by KC-404. These results suggest that the relaxing action of trachea of KC-404 is due to the interference of interaction between LTD4 and its receptor and to the product(s) via cyclooxygenase pathway.
D000818:Animals; D002122:Calcium Chloride / Q000037:antagonists & inhibitors; D002432:Cecum / Q000187:drug effects; D005480:Flurbiprofen / Q000494:pharmacology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D011725:Pyridines / Q000494:pharmacology*; D013189:SRS-A / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014132:Trachea / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*; D007212:Indolizines; D011720:Pyrazoles
D002122:Calcium Chloride; D005480:Flurbiprofen; D006632:Histamine; D011725:Pyridines; D013189:SRS-A; D014665:Vasodilator Agents; C038366:ibudilast; D007212:Indolizines; D011720:Pyrazoles