Variability in humoral responses to DNP-KLH of rainbow trout (Salmo gairdneri). Comparison of antibody kinetics and immunoglobulins spectrotypes between normal trouts and trouts obtained by gynogenesis or self-fertilization.
The anti-DNP antibody responses were compared between conventional trouts produced in fishfarm and trouts experimentally obtained by self-fertilization of hermaphrodites or by gynogenesis. The variability of anti-DNP antibody titres was equivalent between normal and gynogenetic fish, but significantly reduced for fish obtained by self-fertilization. Isoelectrofocusing analysis of individually-isolated anti-DNP antibodies indicate low molecular heterogeneity but large variability between conventional trouts. Almost complete sharing of individual spectrotypes was observed between self-fertilized fish and large similarities between gynogenetics animals. These results suggest that the intensity of antibody synthesis in trout is under a complex genetic control, even in inbred animals expressing large similarities in their antibody repertoires.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000917:Antibody Formation; D005260:Female; D006433:Hemocyanins / Q000276:immunology*; D007178:Inbreeding; D007525:Isoelectric Focusing; D007700:Kinetics; D008297:Male; D010312:Parthenogenesis; D012487:Salmonidae / Q000276:immunology*; D014337:Trout / Q000235:genetics / Q000276:immunology*
C042158:2,4-dinitrophenyl keyhole limpet hemocyanin; D000906:Antibodies; D006433:Hemocyanins