Artificial reductant enhancement of the Lowry method for protein determination.
Addition of dithiothreitol in the Lowry procedure 3 min after adding the Folin-Ciocalteau reagent produces immediate color development, with 35 to 60% greater absorbance per mass of protein used.
D003124:Colorimetry; D004229:Dithiothreitol; D007202:Indicators and Reagents; D008982:Molybdenum; D010084:Oxidation-Reduction; D011506:Proteins / Q000032:analysis*; D013053:Spectrophotometry; D014414:Tungsten; D017973:Tungsten Compounds
C029556:Folin's phenol reagent; D007202:Indicators and Reagents; D011506:Proteins; D017973:Tungsten Compounds; D008982:Molybdenum; D004229:Dithiothreitol; D014414:Tungsten