title: Bacterial infection and acute lung injury in hamsters.
abstract: Bacterial pneumonia is a common complication of lung injury that can be an important determinant of outcome. We studied experimental lung injury produced in hamsters by injecting 20 mg/kg paraquat (PQ) intraperitoneally; control animals received saline vehicle. Three days later, Pseudomonas aeruginosa (PAO1), 10(8) organisms in 0.25 ml, or saline, 0.25 ml, was inoculated intratracheally. Lung and systemic antibacterial defenses were studied at death 24 h later. Paraquat alone produced focal interstitial pneumonitis and neutrophilic alveolitis, and resulted in a 12% (3 of 26) mortality. PAO1 alone caused focal pneumonias and no deaths. Animals receiving both agents (PAO1/PQ) had extensive diffuse alveolar damage characterized by alveolar hemorrhage, edema, influx of neutrophils, and vasculitis; 50% (16 of 32) died within 96 h of PQ injection. Mean lung counts of PAO1 at death were 7.6 X 10(4) colony forming units/g in PAO1 and 2.8 X 10(7) in PAO1/PQ animals (p less than 0.05). PAO1 colony counts in liver were increased nearly 100-fold in PAO1/PQ animals (p less than 0.05). Half-time of clearance of P. aeruginosa from the blood was prolonged in PAO1 and in PAO1/PQ animals (p less than 0.05) but not in PQ animals. Phagocytosis of Staphylococcus aureus by leukocytes lavaged from the lung was not impaired in any group compared with that in control animals, but intracellular killing was impaired in PAO1 and PAO1/PQ but not in PQ animals. Paraquat injury impairs lung antibacterial defenses by uncertain mechanisms. Superinfection of PQ-injured lungs by PAO1 appears responsible for defects in intrapulmonary and systemic antibacterial defenses.
mesh_terms: D000818:Animals; D001770:Blood Bactericidal Activity; D006224:Cricetinae; D005260:Female; D007962:Leukocytes / Q000276:immunology; D008168:Lung / Q000276:immunology; D010269:Paraquat; D010587:Phagocytosis; D011014:Pneumonia / Q000150:complications / Q000276:immunology*; D011552:Pseudomonas Infections / Q000150:complications / Q000276:immunology*; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000150:complications / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology; D013997:Time Factors
chemical_list: D010269:Paraquat