Novel method for substance injection into the cell by laser beam--a study of the injection volume.
A DNA transfection method by laser microbeam pricking has been recently reported (Kurata, S. et al. Exp. Cell Res. 162, 372 (1986]. The volume of external fluid transferred into the cell by the method was determined through the injection of diphtheria toxin fragment A (Yamaizumi, M. et al. Cell 15, 245 (1978]. Using these results and the results on laser DNA transfection efficiency (Kurata, S. et al. Exp. Cell Res. 162, 372 (1986], the approximate number of DNA molecules necessary to transform the recipient cell was estimated.
D000818:Animals; D002460:Cell Line; D004167:Diphtheria Toxin / Q000008:administration & dosage*; D007668:Kidney; D007834:Lasers; D008845:Microinjections / Q000379:methods*; D010446:Peptide Fragments / Q000008:administration & dosage*; D051381:Rats
D004167:Diphtheria Toxin; D010446:Peptide Fragments; C029795:diphtheria toxin fragment A