title: Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.
abstract: The pelB gene, which encodes one of the five pectate lyase isoenzymes of Erwinia chrysanthemi 3937, was mutagenized with a mini-Mu transposable element that can form gene fusions to the neomycin phosphotransferase-encoding region. Secondary mutants resistant to kanamycin in the absence of polygalacturonate, an inducer of wild-type pectate lyase activities, were selected. Such mutants produced other pectate lyase isoenzymes in the absence of the inducer.
mesh_terms: D004885:Erwinia / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation; D011133:Polysaccharide-Lyases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D007527:Isoenzymes; D011133:Polysaccharide-Lyases; C023911:pectate lyase