Identification of the C-terminal flanking peptide of preprocholecystokinin in rat brain by a novel radioimmunoassay.
The C-terminal flanking peptide of preprocholecystokinin (preproCCK) has been identified in extracts of rat brain using a novel radioimmunoassay. There is a single form of immunoreactive material on gel filtration, ion exchange and reversed-phase HPLC. The C-terminal preproCCK immunoreactivity had a similar pattern of distribution to CCK8 in different regions of rat brain. This assay should help in studies of neuronal CCK biosynthesis.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D055598:Chemical Phenomena; D002621:Chemistry; D002766:Cholecystokinin / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats
D009419:Nerve Tissue Proteins; D011498:Protein Precursors; C042082:preprocholecystokinin; D002766:Cholecystokinin