Neutrophils biosynthesize leukotoxin, 9, 10-epoxy-12-octadecenoate.
An epoxy derivative of linoleate, 9,10-epoxy-12-octadecenoate, was demonstrated to be biosynthesized by neutrophils from various sources such as canine and human blood, and guinea-pig peritonea. It was nominated as leukotoxin from its 'toxic' activity onto mitochondrial respiration. From the reaction mixture of leukocytes with linoleate, an isomer of leukotoxin, 12,13-epoxy-9-octadecenoate, and a 'non-toxic' hydroxy derivative of linoleate, 9-hydroxy-12-octadecenoate, were detected. Such a cascade reaction of linoleate by leukocytes was discussed. Biosynthesis of leukotoxin by neutrophils was substantially enhanced by the presence of calcium ion and calcium-ionophore, A23187. Neutrophils contained leukotoxin, ca. 7 f moles/cell, which was extractable by 60% ethanol, but little of the isomer.
D000818:Animals; D002118:Calcium / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004852:Epoxy Compounds / Q000096:biosynthesis*; D004988:Ethers, Cyclic / Q000096:biosynthesis*; D005098:Exotoxins / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D008041:Linoleic Acids / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D005098:Exotoxins; D008041:Linoleic Acids; C018579:leukotoxin; D002118:Calcium