[Detection of urinary polyamine by a new enzymatic differential assay. (II). Comparison with conventional method].
A new method of determining urinary polyamine concentration was compared with other techniques, namely, high pressure liquid chromatography (HPLC) and a polyamine test-enzyme kit. The values obtained by the new method, HPLC, and polyamine test-enzyme kit correlated well for all the fractions: diamine, spermidine and spermine. The correlation between the new method and the polyamine test-enzyme kit gave r = 0.9702, y = 1.1359x + 5.1266 (n = 48).
D002851:Chromatography, High Pressure Liquid; D003959:Diamines / Q000652:urine; D006801:Humans; D008722:Methods; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines / Q000652:urine*; D011933:Reagent Kits, Diagnostic; D013095:Spermidine / Q000652:urine; D013096:Spermine / Q000652:urine
D003959:Diamines; D011073:Polyamines; D011933:Reagent Kits, Diagnostic; D013096:Spermine; D000587:Oxidoreductases Acting on CH-NH Group Donors; D013095:Spermidine