title: Surgical treatment of total colonic aganglionosis: efficacy of aganglionic patch enteroplasty in the rat.
abstract: This study evaluates ileo endorectal pull-through (IEP) with and without an aganglionic colon patch in an experimental rat model for the surgical treatment of total colonic aganglionosis (TCA). Animals were randomly assigned to Group 1 (no patch, No. = 6), Group 2 (right colon patch, No. = 8), Group 3 (transverse colon patch, No. = 7), Group 4 (left colon patch; No. = 6), sham operation (SH, No. = 6), and unoperated controls (UC, No. = 10). Change in percent body weight at 4 weeks after operation was -30.9 +/- 3.68% in Group 1, +5.1 +/- 1.67% in Group 2, -3.4 +/- 3.96% in Group 3, -1.8 +/- 4.17% in Group 4, and +12.7 +/- 1.54% in SH (Group 1 v other groups: P less than .001). Restoration of initial body weight occurred in 100% in Group 2 and SH (8/8 and 6/6, respectively), 50% in Group 4 (3/6), 42.9% in Group 3 (3/7), and 0% in Group 1 (0/6). Transit time (stomach to anus) was significantly shorter in Group 1. All rats in patched groups had a prolonged transit time compared with Group 1. Manometric studies in IEP rats showed favorable anal canal pressures, which were slightly lower than SH and UC. Water and Na+ absorption were significantly greater in patched groups. Rats with a right colon patch (Group 2) showed slightly greater absorption at 4 weeks. These data suggest that an aganglionic colon patch may be an important adjunct in the surgical treatment of TCA.
mesh_terms: D000818:Animals; D001548:Benzalkonium Compounds / Q000494:pharmacology; D003106:Colon / Q000187:drug effects / Q000294:innervation* / Q000601:surgery; D003108:Colonic Diseases / Q000139:chemically induced / Q000401:mortality / Q000601:surgery*; D004195:Disease Models, Animal; D005724:Ganglia / Q000187:drug effects*; D007082:Ileum / Q000601:surgery; D008297:Male; D008722:Methods; D051381:Rats; D012007:Rectum / Q000601:surgery
chemical_list: D001548:Benzalkonium Compounds