[Characteristics of the structural state of DNA in chromatin with short linker].
Various fragments of pigeon brain neuron chromatin with very short linker DNA have been studied by circular dichroism (CD). The DNA structure in core particles of the brain and thymus chromatins is very similar. Linker DNA and a part of core DNA in the mononucleosomes of brain chromatin is extended. This conclusion follows from increasing CD amplitude of the brain mononucleosomes as compared with the corresponding value for thymus mononucleosomes. The internucleosome interactions stabilized the compactness of core DNA in the brain oligonucleosomes. The whole linker DNA of the brain chromatin unlike thymus chromatin is extended at low ionic strength. This fact can explain the brain chromatin ability to form a compact structure with increasing ionic strength.
D000818:Animals; D001923:Brain Chemistry; D002843:Chromatin / Q000032:analysis*; D002942:Circular Dichroism; D010856:Columbidae; D004247:DNA / Q000032:analysis*; D009474:Neurons / Q000032:analysis; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000032:analysis; D051381:Rats; D013950:Thymus Gland / Q000032:analysis
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA
Osobennosti strukturnogo sostoianiia DNK v khromatine s korotkim linkerom.