Interaction between human IgD and ricinus agglutinin.
The results show that monoclonal IgD reacts specifically with ricinus agglutinin (molecular weight 120,000) (RcAI). The reaction between IgD and RcAI was inhibited by galactose and lactose but not by glucose, indicating that the interaction is mediated by galactose containing carbohydrate units located in or near the sigma-hinge region. Affinity chromatography on RcAI-Sepharose 4B was successfully used to isolate monoclonal IgD from the IgD myeloma plasma.
D002846:Chromatography, Affinity; D006801:Humans; D007072:Immunoglobulin D / Q000302:isolation & purification; D037102:Lectins; D009194:Myeloma Proteins / Q000302:isolation & purification; D037121:Plant Lectins; D010947:Plants, Toxic; D012278:Ricinus
D007072:Immunoglobulin D; D037102:Lectins; D009194:Myeloma Proteins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1