An enzyme-linked immunosorbent assay for the antineoplastic agent vincristine.
An enzyme-linked immunosorbent assay for vincristine was developed, based on a new procedure for synthesizing the hapten-protein conjugate. In both the immunogen and the enzyme tracer a spacer group was introduced between the hapten and protein, and the vincristine was coupled at a site far from its functional groups. The antibody produced proved to be exceptionally specific as compared with previous immunoassays for bis-indole alkaloids. Thousandfold antibody dilutions could be used and samples at the femtomole range were assayable. Applications of the method to patient plasma samples and to plant material are described.
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007202:Indicators and Reagents; D011817:Rabbits / Q000276:immunology; D013053:Spectrophotometry; D014750:Vincristine / Q000097:blood*
D007202:Indicators and Reagents; D014750:Vincristine