Bovine pneumonic pasteurellosis: evaluation of interactions between bovine pulmonary lavage cells and Pasteurella haemolytica (biotype A, serotype 1), using a luminol-dependent chemiluminescence assay.
In vitro interactions of bovine pulmonary lavage cells (PLC) and pathogenic isolates of Pasteurella haemolytica biotype A, serotype 1, were examined, using a luminol-dependent chemiluminescence (LDCL) assay. The PLC containing high concentrations of bovine alveolar macrophages were incubated with living and heat-killed P haemolytica at bacteria to PLC ratio of approximately 1:1. Kinetics of the mean LDCL response of bovine PLC to heat-killed P haemolytica cells were characterized by a gradual increase in the amount of light emitted over 150 minutes followed by a slight decrease at 180 minutes. In contrast, the LDCL responses of reaction mixtures containing living P haemolytica were characterized by the development of a maximal response at 60 minutes followed by a continued precipitous decrease in light emission to background values by 150 minutes. Differences were not noticed in the LDCL response of PLC suspensions from the same cow to 3 P haemolytica isolates. In each instance, reaction mixtures containing heat-killed bacteria had a similar LDCL profile that was characterized by continuous production of light over 180 minutes, whereas all reaction mixtures containing living bacteria underwent a precipitous decrease in light emission, which eventually resulted in a complete cessation of chemiluminescence. The PLC suspensions from different cattle did not respond to bacterial stimuli uniformly, with respect to the amplitude or detailed nature of the LDCL profile. The time that lapsed between the addition of living P haemolytica to PLC suspensions and the complete cessation of chemiluminescence varied for different cows.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000503:physiopathology; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D008264:Macrophages / Q000502:physiology*; D010325:Pasteurella / Q000302:isolation & purification; D010326:Pasteurella Infections / Q000503:physiopathology / Q000662:veterinary*; D011650:Pulmonary Alveoli / Q000166:cytology*; D012141:Respiratory Tract Infections / Q000503:physiopathology / Q000662:veterinary*; D007507:Therapeutic Irrigation
D008165:Luminol