Enzymatic microanalysis of glycogen.
A specific and simple enzymatic method for the determination of glycogen in tissue, with a detection limit of about 1 microgram glycogen (6.2 nmol glucosyl residues) is described. Glycogen is converted to 6-phosphogluconate by means of amyloglucosidase, hexokinase, and glucose-6-phosphate dehydrogenase. The increase in NADPH is measured fluorometrically. Muscle tissue (5-20 mg) is hydrolysed in hot KOH (5.4 mol/l), neutralized and analysed. The glycogen-glucosyl content in wet rat diaphragm muscle is about 43 mmol/kg.
D000818:Animals; D005470:Fluorometry; D006003:Glycogen / Q000032:analysis*; D007202:Indicators and Reagents / Q000494:pharmacology; D008297:Male; D008722:Methods; D009132:Muscles / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D007202:Indicators and Reagents; D006003:Glycogen