The effect of fibrinogen sialic acid residues on ex vivo platelet deposition on biomaterials.
The effect of fibrinogen sialic acid residues on platelet deposition onto polymer surfaces was examined using a canine ex vivo shunt model. To test the hypothesis that desialylated fibrinogen may enhance platelet deposition when precoated on biomaterials, canine fibrinogen was desialylated and precoated on polyvinyl chloride (PVC) shunts. When protein-coated PVC shunts were exposed to flowing whole blood, both the native and the desialylated fibrinogen elicited the same profile of platelet deposition. This study indicates that platelet deposition and thrombus formation on biomaterial surfaces is not mediated by a mechanism which involves the sialic acid residues of fibrinogen.
D000818:Animals; D001672:Biocompatible Materials; D004285:Dogs; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D019158:N-Acetylneuraminic Acid; D010973:Platelet Adhesiveness; D011143:Polyvinyl Chloride; D012794:Sialic Acids / Q000378:metabolism*; D013927:Thrombosis / Q000209:etiology
D001672:Biocompatible Materials; D012794:Sialic Acids; D005340:Fibrinogen; D011143:Polyvinyl Chloride; D019158:N-Acetylneuraminic Acid