Characterization of proliposomes.
Photographic evidence of the process of proliposome hydration is provided together with comprehensive particle size analysis of both hydrated dimyristoylphosphatidylcholine: dimyristoylphosphatidylglycerol:ergosterol:amphotericin B proliposomes and egg lecithin:ergosterol:amphotericin B proliposomes using photon correlation spectroscopy and Coulter Counter analysis. Proliposome particle size and the temperature during hydration have been shown to have little effect on subsequent liposome size. A short term stability study of proliposomes indicated that only minor changes in the size distribution profile of the hydrated product are apparent after storage at 20 degrees C for 9 months. Furthermore, no drop in amphotericin B potency was noticed over a 6-month period.
D000666:Amphotericin B / Q000032:analysis; D002626:Chemistry, Pharmaceutical; D008081:Liposomes / Q000032:analysis*; D008854:Microscopy, Electron; D010316:Particle Size; D010713:Phosphatidylcholines / Q000032:analysis; D013696:Temperature
D008081:Liposomes; D010713:Phosphatidylcholines; D000666:Amphotericin B