Optical imaging of cell membrane potential changes induced by applied electric fields.
We report the first imaging of the spatial distributions of transmembrane potential changes induced in nonexcitable cells by applied external electric fields. These changes are indicated by the fluorescence intensity of a charge-shift potentiometric dye incorporated in the cell plasma membrane and measured by digital intensified video microscopy.
D001487:Basidiomycota / Q000502:physiology; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D008564:Membrane Potentials; D018521:Plant Physiological Phenomena; D011523:Protoplasts / Q000502:physiology; D011726:Pyridinium Compounds; D012434:Secale / Q000502:physiology
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C050019:1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine