DC-86-M, a novel antitumor antibiotic. I. Taxonomy of producing organism and fermentation.
A novel antibiotic, DC-86-M was isolated from the culture broth of a new isolate, DO-86, from the soil sample collected in Machida-shi, Japan. The producing organism was found to belong to Streptomycetes, for it formed aerial mycelia and chains of spores and its cell wall analyses revealed the presence of LL-diaminopimelic acid. The morphological, cultural and physiological characteristics of the strain DO-86 resemble closely those of Streptomyces luteogriseus and we concluded that the strain DO-86 could be designated as Streptomyces luteogriseus DO-86. The antibiotic was produced in the fermentation medium consisting of lactose 20 g, glucose 10 g, Pharmamedia 15 g, yeast extract 5 g, meat extract 10 g and CaCO3 2 g per liter of tap water.
D000192:Actinomycetales / Q000145:classification* / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D005285:Fermentation; D010619:Phenazines / Q000096:biosynthesis; D013302:Streptomyces / Q000145:classification* / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D010619:Phenazines; C049724:DC 86M