In vitro response of purified ovine peripheral blood lymphocytes to phytohemagglutinin-M.
Peripheral blood was collected from three normal yearling castrated male lambs. Lymphocytes were isolated by Ficoll-Hypaque gradient centrifugation. Lymphocyte response to phytohemagglutinin was evaluated in an isotopic uptake stimulation test under varying culture conditions in order to standardize the assay. Results were analyzed as log10 counts per minute and stimulation indices. Culture conditions consisting of 2 microL (20 micrograms) phytohemagglutinin-M/culture, 1 X 10(6) cells/mL and a 72 hour incubation period were selected for use in further studies.
D000818:Animals; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012756:Sheep / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism
D010835:Phytohemagglutinins; C047876:phytohemagglutinin-M; D013936:Thymidine