Determination of the molecular size of the hepatic H1-receptor by target size analysis.
The molecular sizes of histamine H1-receptors of rat, rabbit, human, pig, guinea-pig, chicken, dog, and bovine liver were investigated by radiation inactivation and determined to be 100,000 to 160,000 daltons in all the animals examined. Statistical analysis showed that the hepatic H1-receptors have a common size of 128,000 +/- 63,000 daltons. Saturation analysis showed that the [3H]mepyramine binding constant was not changed by irradiation, while the binding capacity decreased with increase in the radiation dose.
D000818:Animals; D002417:Cattle; D002645:Chickens; D004285:Dogs; D005720:Gamma Rays; D006168:Guinea Pigs; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D011738:Pyrilamine / Q000378:metabolism; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D011968:Receptors, Histamine / Q000032:analysis*; D011969:Receptors, Histamine H1 / Q000032:analysis* / Q000528:radiation effects; D013045:Species Specificity; D013552:Swine
D011968:Receptors, Histamine; D011969:Receptors, Histamine H1; D011738:Pyrilamine