Autoradiographic localisation of ascorbic acid-dependent binding sites for [125I]iodocyanopindolol in guinea-pig trachea.
Light microscopic autoradiography showed that the supposedly beta-adrenoceptor-selective radioligand [125I]iodocyanopindolol (I-CYP) bound to sites in both the guinea-pig tracheal epithelium and smooth muscle that were sensitive to propranolol and isoprenaline. Low levels of binding were associated with sub-epithelial mucosal cells. Ascorbic acid caused a concentration-related increase in total I-CYP binding which was predominantly associated with the sub-epithelial mucosa, was not inhibited by propranolol, and was thus not associated with beta-adrenoceptors.
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001345:Autoradiography; D001665:Binding Sites; D001980:Bronchi / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D020120:Iodocyanopindolol; D009130:Muscle, Smooth / Q000378:metabolism*; D010869:Pindolol / Q000031:analogs & derivatives* / Q000378:metabolism; D011869:Radioligand Assay; D014132:Trachea / Q000378:metabolism
D020120:Iodocyanopindolol; D010869:Pindolol; D001205:Ascorbic Acid