3-Methylcholanthrene-induced ethoxyresorufin O-deethylase activity in alveolar type II cells of rabbit lung.
Lungs of 3-methylcholanthrene-treated rabbits and those of untreated rabbits were digested with protease, and the cells were separated into an alveolar type II cell-rich fraction and a Clara cell-rich fraction by the use of counter-flow centrifugal elutriation. Drug oxidation activities of the cell homogenate of each fraction were examined. Marked elevation of ethoxyresorufin O-deethylase activity was found mainly in the cell homogenate of the alveolar type II cell-rich fraction after 3-methylcholanthrene treatment. The activity increased 6-fold with the drug treatment. Coumarin hydroxylase activity was found mainly in the cell homogenates of the Clara cell-rich fraction of the 3-methylcholanthrene-treated and untreated rabbits. The activity increased 3.5-fold with the drug treatment.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1; D065637:Cytochrome P-450 CYP2A6; D004790:Enzyme Induction / Q000187:drug effects; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010088:Oxidoreductases / Q000096:biosynthesis*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000201:enzymology*; D011817:Rabbits
D008748:Methylcholanthrene; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1; D065637:Cytochrome P-450 CYP2A6