Mutagenic properties of N-nitrosopeptides in mammalian cell culture assays.
Two N-nitrosopeptides, N-(N-acetyl-L-prolyl)-N-nitrosoglycine and N-(N-acetylvalyl)-N-nitrosoglycine, were investigated for genetic toxicity towards mammalian cells using an established line of Chinese hamster ovary cells (CHO-K1-BH4). Observations were made on three indices of genetic damage, namely chromosome aberrations, sister chromatid exchange and induction of thioguanine-resistant variants. Treatment of cells with either compound resulted in dose-dependent increases in all indices, indicating that both compounds are direct-acting mutagens.
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D008677:Metaphase; D008770:Methylnitrosourea / Q000633:toxicity; D009152:Mutagenicity Tests; D009602:Nitrosamines / Q000633:toxicity*; D010053:Ovary / Q000187:drug effects*; D012854:Sister Chromatid Exchange / Q000187:drug effects
D009602:Nitrosamines; D008770:Methylnitrosourea; C045273:N-(N-acetylprolyl)-N-nitrosoglycine; C046356:N-(N-acetylvalyl)-N-nitrosoglycine