Stimulation of fatty acid synthesis by 4 beta-phorbol-12-myristate-13-acetate in isolated rat hepatocytes.
The tumor-promoting agent 4 beta-phorbol-12-myristate-13-acetate (TPA) is shown to be a potent stimulator of fatty acid synthesis in isolated rat hepatocytes. The maximal effect of TPA is seen at 10(-6) M, and the concentration for half-maximal effect is ca. 10(-8) M. Stimulation of fatty acid synthesis by TPA is shown not to require the presence of extracellular Ca++. TPA produces a significant increase in lactate and pyruvate accumulation. The possible involvement of protein kinase C in short-term regulation of fatty acid synthesis in the liver is discussed.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010704:Phorbols / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D005227:Fatty Acids; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium