Effects of S-100 proteins on assembly of brain microtubule proteins: correlation between kinetic and ultrastructural data.
Microtubules formed in vitro in the presence of S-100 proteins and micromolar Ca2+ concentrations are fewer in number and longer than those formed in the presence of Ca2+ alone. Moreover, microtubules growing after addition of microtubule fragments to a microtubule protein solution in the presence of S-100 are shorter than those growing in its absence. These data lend support to previous results of kinetic studies indicating that S-100 interferes with both the nucleation and the elongation of microtubules in vitro.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D003078:Colchicine / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008868:Microtubule Proteins / Q000378:metabolism*; D008870:Microtubules / Q000502:physiology / Q000648:ultrastructure; D009418:S100 Proteins / Q000494:pharmacology*
D046911:Macromolecular Substances; D008868:Microtubule Proteins; D009418:S100 Proteins; D003078:Colchicine; D002118:Calcium