title: Phototoxicity of tetracyclines as related to singlet oxygen production and uptake by polymorphonuclear leukocytes.
abstract: The photo-induced singlet oxygen production of six tetracyclines was measured as tryptophan degradation. Demethylchlortetracycline was the most efficient singlet oxygen producer followed by doxycycline. The least efficient producer was minocycline. Doxycycline, however, was the most potent inducer of photodamage to polymorphonuclear leukocytes (PMNLs) followed by demethylchlortetracycline. Accordingly, the singlet oxygen production during irradiation did not correlate with the induction of photodamage to the PMNLs. However, the uptake of doxycycline by the cells was 3 times higher than that of demethylchlortetracycline, and the tetracycline-induced photodamage to the PMNLs correlated with the product of singlet oxygen production during irradiation and the drug uptake by the cells.
mesh_terms: D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010787:Photosensitivity Disorders / Q000139:chemically induced*; D013754:Tetracyclines / Q000633:toxicity*
chemical_list: D005609:Free Radicals; D013754:Tetracyclines; D010100:Oxygen