A method for the preparation of 3-ketoacyl-CoA derivatives.
A new method for the preparation of 3-ketoacyl-CoA thioesters (from malonylsemialdehyde to long-chain and phenylpropanoic 3-keto derivatives) is described. It utilizes the facile, thermodynamically very favorable, hydration of 2-alkynoyl-CoA thioesters by enoyl-CoA hydratase. The method is simple and avoids laborious organic syntheses or the use of multicomponent enzymatic methods.
D000214:Acyl Coenzyme A / Q000138:chemical synthesis*; D004746:Enoyl-CoA Hydratase; D013056:Spectrophotometry, Ultraviolet
D000214:Acyl Coenzyme A; D004746:Enoyl-CoA Hydratase