Purification of Cu-Zn-superoxide dismutase from human erythrocytes by immunoaffinity chromatography. Evidence for the presence of isoelectric heterogeneity.
Cu-Zn-superoxide dismutase (SOD) was prepared in highly purified form from human erythrocytes by immunoaffinity chromatography using anti-human Cu-Zn-SOD goat IgG. The purified SOD had a high specific activity and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. On isoelectric focusing and immunoblotting several bands with enzyme activity and antigenicity were discerned.
D002846:Chromatography, Affinity / Q000379:methods; D003429:Cross Reactions; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D013482:Superoxide Dismutase / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification
D013482:Superoxide Dismutase