Effect of NaCl supplementation of Mueller-Hinton broth on susceptibility of staphylococci to aminoglycosides.
The addition of NaCl to cation-supplemented Mueller-Hinton broth increased the MICs of gentamicin, amikacin, and netilmicin for coagulase-positive and -negative staphylococci in a concentration-dependent manner. At 2% NaCl, geometric mean MICs were elevated 14.1- to 25.6-fold. Mueller-Hinton broth containing added NaCl should not be used for testing the susceptibility of staphylococci to aminoglycosides.
D000583:Amikacin / Q000494:pharmacology; D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004352:Drug Resistance, Microbial; D005839:Gentamicins / Q000494:pharmacology; D009428:Netilmicin / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology*; D013210:Staphylococcus / Q000187:drug effects*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D005839:Gentamicins; D012965:Sodium Chloride; D009428:Netilmicin; D000583:Amikacin