Uptake by neuroblastoma cells of glucosylceramide, glucosylceramide glucosidase, its stimulator protein, and phosphatidylserine.
Serum-free cultured neuroblastoma cells (clone NlE-115) have been shown to absorb emulsified glucosylceramide, glucosylceramide glucosidase, an activator protein for the enzyme, and phosphatidylserine from a synthetic medium. Uptake of the enzyme was augmented by phosphatidylserine, and vice versa. Uptake of the enzyme-lipid complex was further augmented by the activator protein. It appears likely that the activator forms a complex only with the enzyme-lipid complex, not with the individual components. Two uptake mechanisms for the enzyme seem to be involved, one of which (the complex with activator proteins and acidic lipid) is sensitive to mannosyl phosphate groups. Hydrolysis of absorbed glucosylceramide was slow unless the medium was supplemented with the acidic phospholipid or glucosidase. The most rapid disappearance of stored glycolipid took place when the ternary mixture was added to the cell medium, enzyme + activator protein + phosphatidylserine. These findings may be relevant to enzyme replacement therapy for Gaucher disease.
D000818:Animals; D002478:Cells, Cultured; D002554:Cerebrosides / Q000378:metabolism*; D005776:Gaucher Disease / Q000188:drug therapy; D005959:Glucosidases / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D005963:Glucosylceramides / Q000378:metabolism*; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism* / Q000494:pharmacology; D011506:Proteins / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology
D002554:Cerebrosides; D005963:Glucosylceramides; D010718:Phosphatidylserines; D011506:Proteins; C043984:cohydrolase sphingolipid I; D005959:Glucosidases