The production of high affinity monoclonal antibodies to human chorionic gonadotropin and their application to immunoradiometric assay.
Production of monoclonal antibodies against hCG has been studied using hCG as the antigen. This study reports the successful isolation of hybrid clones secreting monoclonal antibodies specific for hCG with an affinity constant higher than 10(10) M-1. Of 23 fusions, only 17 fusions have produced positive clones which secrete antibodies giving high levels of binding with 125I-labelled hCG in the supernatant. Finally, 6 different monoclonal antibodies have been isolated; 4 of them, specific for the beta-subunit, with a Ka approximately 1.1-4.0 X 10(11) M-1 and 2 others, specific for the alpha-subunit, presenting an affinity of 2.5 X 10(10) M-1. When the antibodies specific for the beta-subunit are used, specific and highly sensitive radioimmunoassays are obtained after only 3 hrs of incubation. Using iodinated monoclonal antibodies specific for the alpha-subunit and tubes coated with antibodies against the beta-subunit, we have developed sensitive immunoradiometric assays.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006063:Chorionic Gonadotropin / Q000032:analysis*; D005260:Female; D006825:Hybridomas / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006063:Chorionic Gonadotropin