title: [Multiplicity of affinity modification of RNAse during its alkylation with a reactive analog of 5-deoxyribonucleotide].
abstract: The interaction of pancreatic RNase with 5'-deoxyribodinucleotide alkylating derivative, 4-(N-2-chloroethyl-N-methylamino)benzylamide of d(pTpA) d[(ClRCH2NH)pTpA], was studied. The unreactive oxyanalogue d[(HORCH2NH)pTpA] was shown to act as competitive inhibitor of cCMP hydrolysis by RNase. d[(ClRCH2NH)pTpA] irreversibly inactivated RNase. A protective effect was exerted by d(pTpA) and d[(HORCH2NH)pTpA]. The modification, although having an affinity character, was not accompanied by total inactivation of the enzyme. It was supposed that covalent bonding between the reagent and enzyme induced the dinucleotide displacement from the recognition site. The formation of four RNase monolabeled forms retaining the activity in the hydrolysis of cCMP and poly(U) was demonstrated.
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D003854:Deoxyribonucleotides / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D009150:Mustard Compounds / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors*; D013379:Substrate Specificity
chemical_list: D000477:Alkylating Agents; D003854:Deoxyribonucleotides; D009150:Mustard Compounds; C025881:4(N)-(2-chloroethyl-N-methylamino)benzylamine; D012259:Ribonuclease, Pancreatic
vernacular_title: Mnozhestvennost' affinnoĭ modifikatsii RNKazy pri alkilirovanii ee reaktsionnosposobnym analogom 5'-dezoksiribonukleotida.