Requirement of serum components for the preservation of primordial germ cells in testis cords during early stages of testicular differentiation in vitro in the mouse.
Mouse gonadal primordia were isolated from embryos on the 11th day of gestation and cultured in vitro. They developed into either testes or ovaries after 7 days of culture in Eagle's minimum essential medium (MEM) supplemented with horse serum, whereas they did not differentiate in MEM alone. We studied how serum components are required for testicular development in vitro. When gonadal primordia were cultured in MEM alone for the first 1-3 days and subsequently in MEM supplemented with serum, testis cords developed while germ cells disappeared or only a few remained in the testis cords. In contrast, when serum was present in the medium during the first day of culture and omitted thereafter, germ cells were retained within testis cords. These results suggested that some serum component(s) is specifically required by germ cells independent of testis cord organization. Of more than 10 serum components tested, low and very low density lipoprotein fractions increased the number of germ cells in testicular explants.
D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood; D008297:Male; D051379:Mice; D009924:Organ Culture Techniques; D011247:Pregnancy; D013737:Testis / Q000166:cytology / Q000196:embryology*; D014021:Tissue Preservation
D003470:Culture Media; D008074:Lipoproteins