Effect of serum on inhibition of DNA synthesis in leukemia cells by cis- and trans-(Pt (NH3) 2C1(2)).
We examined the inhibition of DNA synthesis by cis- and trans-diamminedichloroplatinum (II) (cis- and trans-Pt) in leukemia cells, YAC-1 and RADA1. The degree of inhibition by trans-Pt was about the same as that by cis-Pt in vitro in the absence of serum, but the former was much lower than the latter in vivo or in the presence of serum in vitro. Atomic absorption studies showed that the amount of trans-Pt trapped by the serum in vitro is much larger than that of cis-Pt. Therefore, the amount of trans-Pt bound to DNA in vivo must be considerably smaller than that of cis-Pt, which eventually results in the antitumor-inactive nature of trans-Pt.
D000818:Animals; D001769:Blood; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects*; D007536:Isomerism; D007700:Kinetics; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D008805:Mice, Inbred A
D003470:Culture Media; C034697:transplatin; D002945:Cisplatin