Effects of in-vitro capacitation of human spermatozoa on the action of the sperm-immobilizing and sperm-agglutinating antibodies. Possible therapeutic applications on female antisperm isoimmunization.
In 51 serum samples (31 originating from infertile women, 20 from infertile men) with significant sperm immobilizing activity (S.I.T. with S.I.V. greater than 2) we carried out the following tests using semen from three proven fertile donors with excellent semenological characteristics: repetition of S.I.T.; carrying out of S.I.T. with sperm previously subjected to in vitro capacitation (S.I.T.-cap.); carrying out of T.A.T.; carrying out T.A.T. with sperm previously subjected to in-vitro capacitation (T.A.T.-cap.). Nor was there statistically significant difference evident in the results of the tests in relation to the use of the semen of the three different donors. In all 51 samples the repetition of S.I.T. involved the confirmation of sperm-immobilizing activity, while the result of such activity was constantly negative when it was carried out with S.I.T.-cap. In female subjects the comparison of the results of S.I.T. and S.I.T.-cap. showed a statistically significant difference (p less than 0.001). 38 out of the 51 serum samples examined showed positive T.A.T. with variant titers from 1:32 to 1:512 and with different types of agglutination (H, T, tip, M). In all the serum examined, T.A.T.-cap. resulted positive with a titer of 1:512 and agglutination of type H. The results of S.I.T.-cap. seem to underline the advantages linked to the use of capacitated semen in A.I.H. and in G.I.F.T. The evaluation of the results of T.A.T.-cap. make us suspect that they are not linked with the presence of antibodies in tested sera.
D000372:Agglutination Tests; D001323:Autoantibodies / Q000276:immunology*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000276:immunology* / Q000628:therapy; D007248:Infertility, Male / Q000276:immunology* / Q000628:therapy; D007317:Insemination, Artificial, Homologous; D007518:Isoantibodies / Q000276:immunology*; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000276:immunology; D013075:Sperm Capacitation; D013081:Sperm Motility
D001323:Autoantibodies; D007518:Isoantibodies