The phosphorylation of human link proteins.
Three link proteins of 48,44 and 40 kDa were purified from human articular cartilage and identified with monoclonal anti-link protein antibody 8-A-4. Two sets of lower molecular weight proteins of 30-31 kDa and 24-26 kDa also contained link protein epitopes recognized by the monoclonal antibody and were most likely degradative products of the intact link proteins. The link proteins of 48 and 40 kDa were identified as phosphoproteins while the 44 kDa link protein did not contain 32P. The phosphorylated 48 and 40 kDa link proteins contained approximately 2 moles PO4/mole link protein.
D000368:Aged; D000911:Antibodies, Monoclonal; D002358:Cartilage, Articular / Q000032:analysis*; D002850:Chromatography, Gel; D016326:Extracellular Matrix Proteins; D005260:Female; D006801:Humans; D007120:Immunochemistry; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010766:Phosphorylation; D011506:Proteins / Q000302:isolation & purification*; D011509:Proteoglycans; D000099249:Proteoglycan Link Protein
D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D010446:Peptide Fragments; D011506:Proteins; D011509:Proteoglycans; D000099249:Proteoglycan Link Protein