Partial molecular characterization of endothelial cell mitogens from human brain: acidic and basic fibroblast growth factors.
Two endothelial cell growth factors have been isolated from human brain extracts by a procedure involving heparin-Sepharose affinity chromatography and reverse-phase HPLC. On the basis of molecular size, amino acid composition and extended amino-terminal sequences, the two proteins are structurally closely related to bovine acidic and basic fibroblast growth factors (aFGF, bFGF). The N-terminal sequences of human and bovine aFGF differ in two positions while those for bFGF are indistinguishable. Human aFGF is microheterogenous: in addition to aFGF two N-terminally truncated forms lacking the first and the seven first amino acids, respectively, were characterized. Human and bovine mitogens possess similar mitogenic activities.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004727:Endothelium / Q000032:analysis; D005346:Fibroblast Growth Factors / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008934:Mitogens / Q000302:isolation & purification*; D008970:Molecular Weight
D000596:Amino Acids; D008934:Mitogens; D005346:Fibroblast Growth Factors