Rooster comb hyaluronate-protein, a non-covalently linked complex.
Hyaluronate from rooster comb was isolated by ion-exchange chromatography on DEAE-cellulose from tissue extracts and papain digests. The preparations were labelled with [14C]acetic anhydride and subjected to CsCl-density-gradient centrifugation in 4 M-guanidinium chloride in the presence and absence of 4% ZwittergentTM 3-12. A radioactive protein fraction was separated from the hyaluronate when the zwitterionic detergent was also present. The protein could also be separated from the glycosaminoglycan by chromatography on Sepharose CL-6B eluted with the same solvent mixture. The protein fraction contained three protein bands of Mr 15,000-17,000 as assessed by polyacrylamide-gel electrophoresis in 0.1% SDS, and seemed to lack lysozyme activity. No evidence of other protein or amino acid(s) covalently linked with the hyaluronate was obtained. The hyaluronate-protein complex may be re-formed upon mixing the components, the extent of its formation depending on the conditions used. The results show that, as in chondrosarcoma [Mason, d'Arville, Kimura & Hascall (1982) Biochem. J. 207, 445-457] and teratocarcinoma cells [Prehm (1983) Biochem. J. 211, 191-198] the rooster comb hyaluronate also is not linked covalently to a core protein.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002645:Chickens / Q000378:metabolism*; D002850:Chromatography, Gel; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006820:Hyaluronic Acid / Q000378:metabolism*; D046911:Macromolecular Substances; D008297:Male; D011506:Proteins / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds
D003902:Detergents; D046911:Macromolecular Substances; D011506:Proteins; D000644:Quaternary Ammonium Compounds; C034730:zwittergent 3-12; D006820:Hyaluronic Acid