title: Leishmania in the Old World: 1. The geographical and hostal distribution of L. major zymodemes.
abstract: 135 stocks of Leishmania major from man, reservoir hosts and sandflies were characterized using thin-layer starch-gel electrophoresis of 13 enzymes: MDH, 6PGD, GD, SOD, ASAT, ALAT, PK, PGM, ES, NH, PEPD, MPI, GPI. Homogeneity in this species was demonstrated by identical electrophoretic mobilities in nine enzymes. Polymorphism in four enzymes: 6PGD, GPI, PEPD, ES, gave six zymodemes among the collection. Stocks from sandflies and several species of burrowing rodents were indistinguishable from those from man in the same areas. Stocks of Leishmania from North-West India were identified as L. major. In some foci the distribution of zymodemes has some correlation with the presence of particular rodent reservoir hosts. The enzymic homogeneity of L. major throughout its geographical and host range appears to be correlated with the close association between L. major and sandflies of the subgenus Phlebotomus. The status of L. major as a distinct species is supported.
mesh_terms: D000818:Animals; D004197:Disease Reservoirs; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007895:Leishmania tropica / Q000201:enzymology*; D007896:Leishmaniasis / Q000635:transmission; D011110:Polymorphism, Genetic; D011576:Psychodidae; D012377:Rodentia
chemical_list: D007527:Isoenzymes