In vitro ADP-ribosylation of chromosomal proteins of the brain of developing rats.
In vitro ADP-ribosylation of chromosomal proteins and its modulation by spermine, 3-aminobenzamide (3-AB) and benzamide were studied by incubating the nuclei of cerebral hemisphere of 3-, 14- and 30-day old rats with 32P-NAD+. Histones get ADP-ribosylated more than the non-histone chromosomal (NHC) proteins. H1 is the major target for ADP-ribosylation. Among the nucleosomal histones, H2B is ADP-ribosylated most. The other core histones also get ADP-ribosylated to a lesser extent. ADP-ribosylation of both histones and NHC proteins decreases during development. Spermine stimulates, whereas 3-AB and benzamide inhibit, 32P-ADP-ribose incorporation into histones and NHC proteins. These effects decrease with development. Mild digestion of chromatin by micrococcal nuclease (MNase), EcoRI and AluI prior to ADP-ribosylation stimulates incorporation of 32P-ADP-ribose. The degree of stimulation decreases as development proceeds. Such alterations indicate progressive condensation of chromatin with development.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000375:Aging; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008297:Male; D008836:Micrococcal Nuclease / Q000494:pharmacology; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013096:Spermine / Q000494:pharmacology
D001549:Benzamides; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D009702:Nucleoside Diphosphate Sugars; D000246:Adenosine Diphosphate Ribose; D013096:Spermine; C037689:benzamide; C025160:3-aminobenzamide; D008836:Micrococcal Nuclease