Separation of amino acid phenylthiohydantoin derivatives by high-pressure liquid chromatography.
Separation of the phenylthiohydantoin (PTH) derivatives of all 20 common amino acids is accomplished in approximately 11 min with excellent resolution by using high-pressure liquid chromatography. The chromatography is achieved at 50 degrees C on an Altex reversed-phase PTH-C18 column in an ammonium acetate-buffered acetonitrile, pH 4.5, mobile phase. Simple isocratic and linear gradient steps are used. Retention times for the various PTH-amino acids are very reproducible. Because the baseline is flat and free of background noise, PTH-amino acids can be detected in the low picomole range. The simplicity of this chromatographic system allows it to be easily automated.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006827:Hydantoins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008832:Microchemistry; D010669:Phenylthiohydantoin / Q000031:analogs & derivatives / Q000302:isolation & purification*
D000596:Amino Acids; D006827:Hydantoins; D010669:Phenylthiohydantoin