In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.
An in vitro assay for curative antibody present in plasma of mice cured of Trypanosoma musculi is described. The assay involves the addition of plasma to a sample of infected blood, followed by hourly monitoring of the parasite count therein. Immune plasma effected a significant reduction in parasite number, whereas normal mouse plasma did not. Heat treatment of immune plasma for 30 min at 56 degrees C abolished its ability to effect any reduction. The assay is simple, rapid, and economical and correlates well with results of all in vivo studies performed to date.
D000818:Animals; D003165:Complement System Proteins / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004546:Elapid Venoms / Q000494:pharmacology; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014352:Trypanosomiasis / Q000097:blood / Q000276:immunology*
D004546:Elapid Venoms; D007106:Immune Sera; D007136:Immunoglobulins; C016310:cobra venom factor; D003165:Complement System Proteins