The use of DNA-intercalating dye to monitor cell fusion and microinjection.
A conventional method for microinjection, using erythrocyte ghosts as the injection vector, has been modified to provide a protocol for the highly efficient delivery of small quantities of material into the cytoplasm of target cells. The technique is applicable for use with a variety of proteins, sugars, nucleotides and dyes. When the intercalating dye propidium iodide is included within the sealed ghosts their subsequent fusion with target cells can be continuously monitored by fluorescence spectroscopy, providing a convenient and sensitive parameter of cell-cell fusion. The protocol can be adapted for use with both adherent and non-adherent target cells, and can be used to monitor the relative effectiveness of a variety of fusogenic agents.
D002459:Cell Fusion; D004396:Coloring Agents; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007364:Intercalating Agents; D008845:Microinjections; D010617:Phenanthridines; D011419:Propidium; D013050:Spectrometry, Fluorescence
D004396:Coloring Agents; D007364:Intercalating Agents; D010617:Phenanthridines; D011419:Propidium