title: The effect of extracellular matrix modifications on UDP-glucose dehydrogenase activity in cultured human skin fibroblasts.
abstract: The effect of modifications of the extracellular matrix on the biosynthesis of glycosaminoglycans was investigated in human skin fibroblast cultures by studying UDPGDH activity in order to evaluate: a), the histoenzymological and biochemical modifications induced by chondroitinase ABC treatment (new experimental conditions were developed in order to obtain minimum cell damage); b), the reversibility of these modifications; c), the effect of growing the cells in the presence of chondroitinsulfate; d), the specificity of the modifications induced. The results demonstrated that our experimental conditions specifically affected intracellular UDPGDH activity. Chondroitinase ABC treatment induced a reversible increase of UDP-glucuronic acid synthesis. On the contrary, the presence of chondroitinsulfate in the growth medium completely inhibited UDPGDH activity.
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000201:enzymology*; D014018:Tissue Distribution; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D002237:Carbohydrate Dehydrogenases; D014533:Uridine Diphosphate Glucose Dehydrogenase; D007770:L-Lactate Dehydrogenase