title: Improved yield of Mobiluncus species from clinical specimens after alkaline treatment.
abstract: Reference strains and clinical isolates of Mobiluncus species and bacteria in samples of vaginal discharge from patients with bacterial vaginosis were examined for resistance to alkaline solutions. A prolonged survival time in buffers of high pH was shown for Mobiluncus, especially for isolates designated as M. curtisii. In a clinical study, alkaline treatment increased the number of culture-positive samples four times as compared to conventional culture. The improved culture-yield in combination with a shortened time for processing the samples makes the method suitable for isolation of Mobiluncus in clinical samples.
mesh_terms: D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D002021:Buffers; D003470:Culture Media; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D014621:Vagina / Q000382:microbiology*; D014627:Vaginitis / Q000382:microbiology*
chemical_list: D002021:Buffers; D003470:Culture Media