Modulation of the catalytic subunit of cyclic AMP-dependent protein kinase by calmodulin, S-100 protein, parvalbumin and troponin.
The activity of the catalytic subunit of cyclic AMP-dependent protein kinase (A-PK), utilizing type II-S, III-S histone or protamine (free base) as a substrate, was augmented in the presence of regulatory protein including calmodulin, S-100 protein, parvalbumin, or troponin. However, inhibition by calmodulin or S-100 but stimulation by parvalbumin or troponin on this A-PK subunit was observed when II-S histone was replaced by V-S, VI-S, VII-S, or VIII-S histone. In addition, the stimulatory effect of calmodulin or S-100 on this A-PK subunit utilizing III-S histone was greatly diminished when half the dose of III-S was replaced by other histones in a bi-mixed form.
D000818:Animals; D002147:Calmodulin / Q000494:pharmacology*; D002417:Cattle; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D008274:Magnesium / Q000494:pharmacology; D009124:Muscle Proteins / Q000494:pharmacology*; D010320:Parvalbumins / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D009418:S100 Proteins / Q000494:pharmacology*; D013379:Substrate Specificity; D014336:Troponin / Q000494:pharmacology*
D002147:Calmodulin; D006657:Histones; D046911:Macromolecular Substances; D009124:Muscle Proteins; D010320:Parvalbumins; D009418:S100 Proteins; D014336:Troponin; D011494:Protein Kinases; D008274:Magnesium