Abnormal expression of the calmodulin gene in muscle from the dystrophic chicken.
Compared to that of genetically-related normal chickens, pectoralis muscle from the dystrophic chicken contained increased calmodulin measured by radioimmunoassay. Determined by the dot blot procedure, expression of the calmodulin gene was enhanced in muscle from affected animals. The bioactivity of the gene product was normal. Together with previous studies reporting increased cell Ca2+ content in dystrophic muscle, the current findings of increased sarcoplasmic calmodulin suggest the latter is a cellular response to defective Ca2+ transport at the level of cell efflux or intracellular organelle (sarcoplasmic reticulum) uptake.
D000818:Animals; D002147:Calmodulin / Q000235:genetics*; D002645:Chickens; D005786:Gene Expression Regulation; D009132:Muscles / Q000502:physiology; D009137:Muscular Dystrophy, Animal / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics
D002147:Calmodulin; D012333:RNA, Messenger