Pertussis vaccine reduces agonist binding to the rat heart muscarinic receptor and its guanine nucleotide modulation.
The injection of Bordetella pertussis, inactivated by merthiolate, causes a 2-fold increase in the IC50 of carbamylcholine (carbachol) in displacing [3H];-L(-) quinuclidinyl benzilate binding ([3H]QNB) to the receptor. In control animals, 50 microM Gpp(NH)p causes a 6-fold decrease in the affinity of carbachol binding, whereas after vaccination the reduction is only 1.6-fold. After pertussis treatment there is no alteration in the affinity and number of [3H]QNB binding sites of to the muscarinic receptor.
D000818:Animals; D002217:Carbachol / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010567:Pertussis Vaccine / Q000494:pharmacology*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*
D010567:Pertussis Vaccine; D011976:Receptors, Muscarinic; D006165:Guanylyl Imidodiphosphate; D011813:Quinuclidinyl Benzilate; D006160:Guanosine Triphosphate; D002217:Carbachol