Nature of the binding of activated cyclophosphamide to calf thymus DNA and pBR-322 plasmid DNA.
Differentially radiolabeled cyclophosphamide (CP) was metabolized with a reconstituted cytochrome P-450 system in the presence of calf thymus DNA or plasmid pBR-322 DNA. The isolated DNA was subjected to agarose gel electrophoresis and the associated radioactivity was determined. The majority (greater than 90%) of the initial DNA-bound radioactive metabolites dissociated from the DNA upon gel electrophoresis. The results are discussed in context to the binding of activated CP to DNA and the nature of the binding.
D000818:Animals; D001711:Biotransformation; D003520:Cyclophosphamide / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism; D013950:Thymus Gland
D004269:DNA, Bacterial; D003520:Cyclophosphamide; D004247:DNA; D003577:Cytochrome P-450 Enzyme System