Identification of a disulfide-bonded 70 Kd type X procollagen in embryonic chick sternum cartilage.
Chondrocytes from the presumptive calcification region of 20 day old embryonic chick sternum were found to synthesize a 70 Kd Type X procollagen precursor in addition to the previously described 59 Kd Type X collagen molecules. The 70 Kd molecules exhibited an additional cyanogen bromide peptide, contained a disulfide-bonded domain, and were converted into the 59 Kd moieties during pulse-chase experiments. The conversion of the 70 Kd to the 59 Kd Type X collagen was prevented upon microtubular transport inhibition with colchicine and resulted in tissue accumulation of the 70 Kd Type X procollagen.
D000818:Animals; D002113:Calcification, Physiologic; D002250:Carbon Radioisotopes; D002356:Cartilage / Q000196:embryology / Q000378:metabolism*; D002642:Chick Embryo; D004220:Disulfides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008970:Molecular Weight; D009924:Organ Culture Techniques; D011347:Procollagen / Q000096:biosynthesis* / Q000302:isolation & purification; D011392:Proline / Q000378:metabolism; D013249:Sternum
D002250:Carbon Radioisotopes; D004220:Disulfides; D011347:Procollagen; D011392:Proline