title: Formation sites and distribution of osteoclast progenitor cells during the ontogeny of the mouse.
abstract: The presence of osteoclast progenitor cells in embryonic, fetal, young growing, and adult murine tissues and organs was investigated in a coculture system with fetal metatarsal bones stripped of periosteum and not yet invaded by osteoclasts. Osteoclasts were found to originate from the early yolk sac and from every tissue tested in the fetus and young mouse. In the adult mouse they were formed only from tissues with a large mononuclear phagocyte population. No osteoclasts could be generated from the young embryo proper, prior to establishment of the vascular connection with the yolk sac. Progenitors of osteoclasts or their stem cells therefore do not develop from undifferentiated mesenchyme outside the yolk sac, but are distributed from the yolk sac to embryonic tissues and hematopoietic organs through the vascular circulation. The embryonic distribution of osteoclast progenitors coincides with the distribution of immature macrophages. Furthermore, they are present before the formation of monocytes in the fetus. The results also indicate that osteoclast precursor cells are not identical with mature, differentiated macrophages, but are cells with little capacity to phagocytose and therefore are, at the most immature progenitors of macrophages or cells of an early diverging lineage. In view of these results the derivation of osteoclasts is discussed.
mesh_terms: D000367:Age Factors; D000818:Animals; D001842:Bone and Bones / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000196:embryology; D051379:Mice / Q000196:embryology / Q000254:growth & development; D010010:Osteoclasts / Q000166:cytology*; D013154:Spleen / Q000166:cytology / Q000196:embryology; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology; D014018:Tissue Distribution; D015017:Yolk Sac / Q000166:cytology