Large-scale procedure for the purification of fibronectin domains.
Human plasma fibronectin is composed of at least seven distinct domains, with affinities for different macromolecules and cell surfaces. Here we describe in detail a simple high-yield procedure for the purification of large amounts of fibronectin domains. This involves thermolysin digestion of the fibronectin molecule followed by the purification of the domain using mainly hydroxyapatite chromatography columns. This procedure represents a great simplification over those previously reported.
D000596:Amino Acids / Q000032:analysis; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D010446:Peptide Fragments / Q000302:isolation & purification; D010710:Phosphates; D013820:Thermolysin
D000596:Amino Acids; D005353:Fibronectins; D010446:Peptide Fragments; D010710:Phosphates; D013820:Thermolysin; D008274:Magnesium