title: [Study of human erythrocyte membrane proteins by 2-dimensional electrophoresis].
abstract: Fractionation of human erythrocyte membrane proteins was performed using a modification of two-dimensional gel electrophoresis described by P. O'Farrel with isoelectric point plotted against molecular mass. All major erythrocyte proteins, including high molecular weight proteins, such as spectrin and band 3 protein, identified by one-dimensional sodium dodecyl sulfate gel electrophoresis, were visualized by silver staining of two-dimensional gels. All in all about 50 polypeptides were distinguished on two-dimensional electrophoretic patterns. Preliminary protein map was developed.
mesh_terms: D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D004910:Erythrocyte Membrane / Q000032:analysis*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*
chemical_list: D001798:Blood Proteins; D008565:Membrane Proteins
vernacular_title: Izuchenie belkov membran éritrotsitov cheloveka metodom dvukhmernogo élektroforeza.