title: Spectral shift of fluorescein and carboxyfluorescein in the anterior chamber of the rabbit eye following systemic administration.
abstract: Systemically administered fluorescein is converted to fluorescein glucuronide. The conjugate is fluorescent and interferes with the measurement of fluorescein in the anterior chamber. Carboxyfluorescein is a hydrophilic derivative of fluorescein. In the rabbit, carboxyfluorescein is not converted as readily to a fluorescent metabolite. Thus, carboxyfluorescein has potential advantages as a quantitative fluorophore for studies of aqueous humor dynamics.
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism*; D002845:Chromatography; D019793:Fluorescein; D005452:Fluoresceins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005453:Fluorescence; D005470:Fluorometry; D007275:Injections, Intravenous; D010783:Photometry; D011817:Rabbits
chemical_list: D005452:Fluoresceins; C024098:6-carboxyfluorescein; D019793:Fluorescein