The detection of leptospires by a chemiluminescent immunoassay.
Rabbit serum hyperimmune to Leptospira interrogans var icterohaemorrhagiae serovar icterohaemorrhagiae, reference strain RGA was conjugated to the chemiluminescent label ABEI (6-[N-(4-aminobutyl)-N-ethyl amino]-2,3-dihydrophthalazine-1,4-dione) in the presence of the coupling agent, EDC (1-ethyl-3(3-dimethyl amino-propyl)-carbodiimide HCl). The luminescent conjugate was incubated with homologous and heterologous antigens. The results indicate that chemiluminescence may provide an accurate and rapid method of detecting leptospires in biological fluids.
D000372:Agglutination Tests; D000818:Animals; D007106:Immune Sera; D007118:Immunoassay / Q000379:methods; D007919:Leptospira / Q000302:isolation & purification*; D007921:Leptospira interrogans / Q000276:immunology; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives; D011817:Rabbits / Q000276:immunology
D007106:Immune Sera; D008165:Luminol; C031912:6-(N-(4-aminobutyl)-N-ethyl)amino-2,3-dihydrophthalazine-1,4-dione