title: Inhibitory effects of danazol on steroidogenesis in cultured human granulosa cells.
abstract: The effect of danazol on steroidogenesis in cultured human granulosa cells was studied. Granulosa cells obtained from antral follicles in the mid to late follicular phase were cultured for 2 to 6 days. Danazol (0.1 to 5 micrograms/ml) and human chorionic gonadotropin (hCG, 10 IU/ml), alone or in combination, were added to the culture medium. Testosterone (T) (1 microgram/ml) was added as an aromatase substrate in certain experiments. The medium content of progesterone (P) and estradiol (E2) was determined. Basal P secretion was variably influenced by danazol, whereas a consistent dose-dependent and reversible inhibition of the hCG-stimulated P secretion was found. In the presence of T, danazol caused a dose-dependent inhibition of both basal and hCG-stimulated E2 secretion, and this effect became more pronounced with time. The results demonstrate that danazol exerts direct inhibitory effects on steroidogenesis in cultured human granulosa cells.
mesh_terms: D000328:Adult; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D006224:Cricetinae; D003613:Danazol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects*; D006801:Humans; D008875:Middle Aged; D011245:Pregnadienes / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis; D051381:Rats; D013256:Steroids / Q000096:biosynthesis*; D013552:Swine; D013739:Testosterone / Q000494:pharmacology
chemical_list: D006063:Chorionic Gonadotropin; D011245:Pregnadienes; D013256:Steroids; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D003613:Danazol