Rapid tumor regression caused by antitumor polysaccharide and induction of tumor-regressing factor in the serum of tumor-bearing mice.
The antitumor polysaccharide CM-TAK [carboxymethylated beta(1-3)glucan] caused immediate and rapid loss of viable tumor cells from S180 solid tumors in ICR mice if it was given after a week of tumor growth. We found marked tumor-regressing activity in the serum of mice with S180 tumors undergoing CM-TAK-induced regression. When injected intravenously into S180-bearing mice, 0.1 ml of serum/mouse induced complete regression of day-14 tumors. However, in vitro cytotoxic activity against L929 and S180 cells was not detected. After the injection of CM-TAK or serum, along with the rapid decrease in tumor cells there was a marked increase in polymorphonuclear leukocytes. The serum was also active against fibrosarcoma Meth A in BALB/c mice, inducing partial regression or significant decrease in growth of the tumor. The properties of the factor in the serum seem to be different from those reported for tumor-necrotizing factor and other endogenous cytotoxic substances.
D000818:Animals; D001790:Blood Physiological Phenomena; D005260:Female; D005354:Fibrosarcoma / Q000188:drug therapy* / Q000473:pathology; D006023:Glycoproteins / Q000097:blood; D007739:L Cells; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009336:Necrosis; D009504:Neutrophils / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology / Q000627:therapeutic use*; D012510:Sarcoma 180 / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D011135:Polysaccharides, Bacterial; D014409:Tumor Necrosis Factor-alpha