The effects of time of equilibration with cryoprotectants at 0 degree C prior to freezing on the survival of mouse embryos frozen by the two-step method.
Mouse embryos were frozen by the two-step method after equilibration for 0.1-60 min with cryoprotectants at 0 degree C. No survival or a very low survival was obtained after equilibration for only 0.1 min. The morulae showed the highest survival rates when equilibration time was 5-30 min with 2 M DMSO, 20-30 min with 2 M glycerol, 5-10 min with 2 M ethylene glycol and 20-30 min with 2 M propylene glycol, respectively.
D000818:Animals; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D004622:Embryo, Mammalian / Q000502:physiology*; D019855:Ethylene Glycol; D005026:Ethylene Glycols; D005260:Female; D005615:Freezing; D005990:Glycerol; D051379:Mice; D008813:Mice, Inbred ICR; D011309:Preservation, Biological / Q000379:methods*; D019946:Propylene Glycol; D011409:Propylene Glycols; D013997:Time Factors
D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D011409:Propylene Glycols; D019946:Propylene Glycol; D019855:Ethylene Glycol; D005990:Glycerol; D004121:Dimethyl Sulfoxide