[Characteristics of the heterogeneity of the physical properties of the lipid phase of synaptic membranes from the rat brain using the fluorescent probe pyrene].
A theoretical analysis of the dependence of pyrene excimerization on its concentration in biological membranes was carried out. It was shown that in synaptic membranes the concentration dependencies of pyrene excimerization parameter upon direct stimulation appear as nonlinear, thus being reflective of the heterogeneity of physical properties of the lipid phase. Upon pyrene excitation at the expense of the energy transfer from tryptophanyl residues the dependence is linear, which points to the homogeneity of the anular lipid pool. It was assumed that a comparison of microviscosity of the anular and bilayer lipids requires independent measurements of the coefficient of the probe distribution between the lipids or of the anular lipid content in the membrane.
D000818:Animals; D001923:Brain Chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005456:Fluorescent Dyes; D007700:Kinetics; D008563:Membrane Lipids / Q000032:analysis*; D011721:Pyrenes; D051381:Rats; D013050:Spectrometry, Fluorescence; D013570:Synaptic Membranes / Q000032:analysis*
D005456:Fluorescent Dyes; D008563:Membrane Lipids; D011721:Pyrenes; C030984:pyrene
Kharakteristika geterogennosti fizicheskikh svoĭstv lipidnoĭ fazy sinapticheskikh membran iz mozga krys s pomoshch'iu fluorestsentnogo zonda pirena.