Isoenzymic analysis of fifteen non-lysosomal enzymes in human chorionic villi.
The electrophoretic pattern of 15 non-lysosomal enzymes was established in human chorionic villi sampled at 8-12 weeks of gestation from 30 women requesting legal abortion. The product of 23 genes was detected at this stage of development. Trophoblast cells are mitotic derivates from the zygote. Thus, chorionic villi have the same genetic constitution as the embryo. Establishing the normal isoenzymatic patterns in fetal life is of wide theoretical and biological interest. In particular, it contributes to the understanding of gene modulation and the differential activity of alleloenzymes during early embryogenesis and development. In addition, the possibility is suggested that in the future electrophoresis analysis can be used in conjunction with quantitative enzyme assay for the fetal diagnosis of specific metabolic disorders involving one or more isoenzymes.
D002824:Chorionic Villi / Q000201:enzymology*; D004592:Electrophoresis, Starch Gel; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D011247:Pregnancy
D007527:Isoenzymes