Study of a hydrophobic site on bovine alpha-lactalbumin by labeling with [125I]-TID.
The hydrophobic, photoreactive probe 3-(trifluoromethyl)-3-(m-[125I]iodophenyl) diazirine ([125I]TID) labels apo-bovine alpha-lactalbumin but much less his Ca2+-form. The labeling of the apo-form is strong at protein concentrations of 0.5 mg ml-1 and increases with increasing concentration. Furthermore, increasing concentrations of NaCl, decrease the labeling of apo-alpha-lactalbumin with [125I]TID.
D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D001389:Azirines / Q000378:metabolism*; D002417:Cattle; D007457:Iodine Radioisotopes; D007700:Kinetics; D007768:Lactalbumin / Q000378:metabolism*; D009994:Osmolar Concentration; D011485:Protein Binding; D012965:Sodium Chloride / Q000494:pharmacology
D001059:Apoproteins; D001389:Azirines; D007457:Iodine Radioisotopes; D012965:Sodium Chloride; C033174:3-(trifluoromethyl)-3-(3-iodophenyl)diazirine; D007768:Lactalbumin