title: Epithelium removal increases the reactivity of human isolated tracheal muscle to methacholine and reduces the effect of verapamil.
abstract: Removal of the epithelium increased the sensitivity of human isolated tracheal smooth muscle to methacholine, producing a greater than 2-fold leftward shift in the concentration-response curve. Concomitantly, the ability of verpamil to lower the maximum contractile response was reduced in tissues without an intact epithelium. These findings suggest a role for the epithelium in modulating the reactivity of human and extend the findings of similar studies in experimental animals.
mesh_terms: D000328:Adult; D004848:Epithelium / Q000502:physiology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008688:Methacholine Compounds / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects*; D014132:Trachea / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D008688:Methacholine Compounds; D014700:Verapamil