Cell-free synthesis of delta-(L-alpha-aminoadipyl)-L-cysteine, the first intermediate of penicillin and cephalosporin biosynthesis.
delta-(L-alpha-aminoadipyl)-L-cysteine synthetase (LL-AC synthetase) activity has been found in extracts of Cephalosporium acremonium C-10. The enzyme extract carries out a linear synthesis of LL-AC from its constituent amino acids for at least 6 hours. The reaction is dependent on active enzyme, time, L-alpha-aminoadipate, L-cysteine, ATP and Mn2+ or Mg2+. The activity is stabilized by glycerol.
D000164:Acremonium / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002474:Cell-Free System; D002511:Cephalosporins / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000096:biosynthesis*; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D010406:Penicillins / Q000096:biosynthesis*; D013696:Temperature
C039168:5-(2-aminoadipyl)cysteine; D002511:Cephalosporins; D004151:Dipeptides; D010406:Penicillins; D008345:Manganese; D000255:Adenosine Triphosphate; D008274:Magnesium