[Oxidative phosphorylation, NADH-oxidase and succinate oxidase activity in testicular mitochondria of rats with alloxan diabetes].
Decrease in V4P, V3 and VP, in coefficient of respiratory control, in the ratio ADP:0 as well as an increase in duration of phosphorylation of ADP involved in oxidation of succinate were found in testes mitochondria from rats with alloxan diabetes (content of glucose 20 +/- 2 mmol/L in blood plasma). If a mixture of malate and glutamate was used as a substrate of oxidation, the rate of mitochondrial respiration and coefficient of respiratory control were decreased in testes of diabetic rats under conditions of all the metabolic states studied. At the same time, sensitivity of NADH-oxidase and succinate oxidase systems to controlled heating (28 degrees) was distinctly decreased in the testes mitochondrial membranes of the impaired animals as compared with controls. The data obtained suggest that distinct functional and, apparently, structure alterations occurred in the testes mitochondrial membranes of rats with alloxan diabetes.
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010088:Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D013737:Testis / Q000201:enzymology / Q000378:metabolism*
D009097:Multienzyme Complexes; D010088:Oxidoreductases; C022691:succinate oxidase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases
Okislitel'noe fosforilirovanie, aktivnost' NADH-oksidaznoĭ i suktsinatoksidaznoĭ sistem v mitokhondriiakh testikul krys pri alloksanovom diabete.