Interactions of polyclonal anti-Electrophorus nicotinic receptor antisera with Torpedo nicotinic receptor.
Polyclonal antisera raised against solubilized and purified nicotinic acetylcholine receptor (nAcChoR) from Electrophorus electroplax and a polyclonal anti-alpha-bungarotoxin antiserum have been studied by the use of four different radioimmunoassay protocols. The results indicate unique sensitivities of different assay techniques in analysis of antibody-antigen interactions, and serve as a model for immunological study of other integral membrane proteins.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002462:Cell Membrane / Q000378:metabolism; D004557:Electric Organ / Q000378:metabolism; D004593:Electrophorus; D007700:Kinetics; D011978:Receptors, Nicotinic / Q000276:immunology* / Q000302:isolation & purification
D000906:Antibodies; D000936:Antigen-Antibody Complex; D011978:Receptors, Nicotinic