Solubilization of the D-1 dopamine receptor from rat striatum.
The D-1 dopamine receptor was extracted from rat striatal membranes with 0.7% sodium cholate and 1 M NaCl. Pretreatment of the membranes with a D-1 specific agonist, inclusion of crude phospholipids in the solubilization buffer, and subsequent removal of the detergent led to a maximal extraction of 48% of the receptor binding sites. The D-1 antagonist, [125I]SCH 23982, bound to single class of sites with a Kd of 1.8 nM and a Bmax of 1.65 pmol/mg protein. The solubilized receptors retained the ability to discriminate between active and inactive enantiomers of agonists and antagonists selective for the D-1 receptor.
D000818:Animals; D001552:Benzazepines / Q000031:analogs & derivatives* / Q000378:metabolism; D001667:Binding, Competitive; D002793:Cholic Acids; D003342:Corpus Striatum / Q000032:analysis*; D018492:Dopamine Antagonists; D008297:Male; D051381:Rats; D011954:Receptors, Dopamine / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility
C049558:8-iodo-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-3-benzazepine-7-ol; D001552:Benzazepines; D002793:Cholic Acids; D018492:Dopamine Antagonists; D011954:Receptors, Dopamine