Bulk immunopurification and development of a radioimmunoassay for human and murine F liver protein.
By taking advantage of the cross-reaction for human F liver protein (F antigen) of a monoclonal antibody raised to mouse F antigen, human F antigen has been immunopurified in bulk. The molecular weight is 44 000. Using this material as a standard, affinity-purified polyclonal anti-F antibody on a solid phase and radiolabelled monoclonal anti-F antibody to detect bound protein, a capture immunoradiometric assay capable of detecting down to 1 ng X ml-1 of F antigen has been developed. Using this assay, the average level of F antigen in normal mouse serum (CBA strain) is 16 ng X ml-1 (360 pM) and in human serum 10 ng X ml-1 (250 pM).
D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007519:Isoantigens / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D008099:Liver / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D007519:Isoantigens; C030497:liver specific F antigen