Ethanol-induced cell damage in cultured rat antral mucosa assessed by chromium-51 release.
We have developed an in vitro method for studying ethanol-induced injury to gastric mucosa using organ culture of rat antrum. Cell damage was assessed by measurement of the release of [51Cr]sodium chromate from preloaded cells, a method adapted from a standard immunologic technique. This system provided rapid and highly reproducible quantitation of tissue injury as assessed by 51Cr release into the culture medium. The threshold concentration for ethanol-induced damage was between 10 and 15% v/v, similar to in vivo thresholds observed by others. 51Cr release could also be induced by very short exposure to ethanol (5-15 min), and then continued despite ethanol removal. Interestingly, after continuous ethanol exposure, a plateau of maximum 51Cr release was reached 60 min after exposure to ethanol over the concentration range 20-50%, suggesting tissue adaptation to ethanol damage. This organ culture system, which allows precise control of experimental conditions, may be useful for studying mechanisms of gastric mucosal injury and protection.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002840:Chromates; D002860:Chromium Radioisotopes / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009924:Organ Culture Techniques; D011706:Pyloric Antrum / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D017670:Sodium Compounds; D013997:Time Factors
D002840:Chromates; D002860:Chromium Radioisotopes; D017670:Sodium Compounds; D000431:Ethanol; C028982:sodium chromate(VI)