Lipid associated tissue ferritin.
Delipidation of liver homogenates, using an organic solvent system which does not denature proteins, increases measurable ferritin by 25-33%, compared to ferritin concentrations by standard heat supernatant assays. When applied to polyacrylamide gradient gels, lipid-associated ferritin does not enter the gel but after delipidation, this ferritin co-migrates with cytosolic ferritin and with purified liver ferritin. The biological significance of the association of ferritin with lipid has yet to be examined.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000032:analysis* / Q000302:isolation & purification; D006168:Guinea Pigs; D006358:Hot Temperature; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008297:Male; D051381:Rats
D008055:Lipids; D005293:Ferritins