[A new method of isolating lysyl-tRNA-synthetase from the rat liver].
The methods of gel filtration on sepharose 6B, hydrophobic chromatography, chromatofocusing were used to isolate the preparation of lysyl-tRNA-synthetase. The enzyme activity at the terminal stage of purification is 1800 times as high. The isolated preparation is homogeneous with electrophoresis in 4-30% PAAG.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D008250:Lysine-tRNA Ligase / Q000302:isolation & purification*; D008297:Male; D008722:Methods; D051381:Rats
D000604:Amino Acyl-tRNA Synthetases; D008250:Lysine-tRNA Ligase
Novyĭ metodicheskiĭ podkhod k vydeleniiu lizil-tRNK-sintetazy iz pecheni krys.