Characterization and androgen dependence of specific proteins in the epididymis of adult rhesus monkey (Macaca mulatta).
The androgen dependence and regional distribution of specific epididymal proteins have been investigated in the adult rhesus monkey (Macaca mulatta). The protein pattern of the epididymal cytosol was studied in intact, castrated, and testosterone-treated castrated monkeys by polyacrylamide gel electrophoresis (PAGE) and electrofocusing. At least five distinct protein components of the epididymal cytosol were androgen-sensitive. The PAGE of cytosol from various portions of the epididymis showed that whereas four protein components were uniformly distributed in the caput, corpus, and caudal segments, one of the androgen-dependent proteins was found to be associated with the caudal portion of the epididymis. Electrofocusing of epididymal cytosol also confirmed the presence of five androgen-dependent proteins in the adult monkey. The isoelectric points of these proteins were shown to range between 5.6 and 6.5, and the molecular weights were found to range between 15,000 and 61,000.
D000728:Androgens / Q000502:physiology*; D000818:Animals; D003600:Cytosol / Q000032:analysis; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000032:analysis*; D007525:Isoelectric Focusing; D008253:Macaca mulatta; D008297:Male; D009919:Orchiectomy; D011506:Proteins / Q000302:isolation & purification*; D013739:Testosterone / Q000494:pharmacology
D000728:Androgens; D011506:Proteins; D013739:Testosterone