The molecular structure of a rapidly formed oligomeric adenosine tetraphosphate derivative from rat heart.
The inability to account for large systematic variations with time in soluble adenine nucleotides in perfused rat hearts [Bates, Perrett & Mowbray (1978) Biochem. J. 176, 485-493; Mowbray, Bates & Perrett (1981) FEBS Lett. 131, 55-59; Mowbray, Perrett & Bates (1984) Int. J. Biochem. 16, 889-894] led us to show that the soluble nucleotides are in rapid equilibrium with some hitherto unrecognized trichloroacetic acid/methanol-precipitable highly phosphorylated heteropolymeric form [Mowbray, Hutchinson, Tibbs & Morris (1984) Biochem. J. 223, 627-632]. Selective digestion coupled to chromatographic analysis together with m.s. and 31P-n.m.r. spectrometry have now been used to show that the likely structure for a purified oligomer that is in specific-radioactivity equilibrium with tissue ATP is 3-phospho-[glyceroyl-gamma-triphosphoroyl-5'-adenosine-3'-3- phospho]4 glyceroyl-gamma-triphosphoroyl-5'-adenosine.
D000227:Adenine Nucleotides / Q000302:isolation & purification; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005988:Glyceric Acids / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009206:Myocardium / Q000032:analysis*; D009843:Oligoribonucleotides / Q000302:isolation & purification; D010710:Phosphates / Q000032:analysis; D011687:Purines / Q000032:analysis; D051381:Rats
D000227:Adenine Nucleotides; D002241:Carbohydrates; D002264:Carboxylic Acids; D005988:Glyceric Acids; D009843:Oligoribonucleotides; D010710:Phosphates; D011687:Purines