Dehydroascorbic acid and cell membranes: possible disruptive effects.
Exposure of cellular membranes to dehydroascorbic acid can result in a loss of membrane integrity. Renal brush border or basolateral membrane vesicles pre-incubated with dehydroascorbic acid demonstrate a decrease in initial transport rates of D-glucose and a loss of intravesicular volume. The activity of brush border membrane specific leucine aminopeptidase is increased in vesiculated membrane preparations following exposure of the vesicles to either dehydroascorbic acid or Triton X-100. Erythrocytes in isotonic buffer with dehydroascorbic acid lose membrane integrity as demonstrated by a release of hemoglobin.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D003683:Dehydroascorbic Acid; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008871:Microvilli / Q000187:drug effects; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats
D011092:Polyethylene Glycols; D017830:Octoxynol; D007931:Leucyl Aminopeptidase; D005947:Glucose; D003683:Dehydroascorbic Acid