A cycling assay for the determination of mono(adenosine-5'-diphosphoribose)-protein bound conjugate levels in mouse tissues.
Adenosine-5'-diphosphoribose (ADPR) is quantitatively split into 5'-AMP and ribose phosphate by treatment with alkali at elevated temperature. The 5'-AMP is used to generate NAD through a series of enzyme-catalyzed reactions. The NAD is then determined with a cycling assay modified after E.L. Jacobson and M.K. Jacobson [(1976) Arch. Biochem. Biophys. 175, 627-634]. The specificity of this assay has been verified. With this method the levels of mono(ADPR)-protein bound conjugate in various mouse tissues have been determined.
D000246:Adenosine Diphosphate Ribose / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D005260:Female; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D051379:Mice; D009206:Myocardium / Q000032:analysis; D009243:NAD / Q000032:analysis; D009702:Nucleoside Diphosphate Sugars / Q000032:analysis*; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D013696:Temperature
D009702:Nucleoside Diphosphate Sugars; D011506:Proteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D000249:Adenosine Monophosphate