[Effect of adrenaline, hydrocortisone and serum HDL lipoproteins on the activity of multiple forms of glucose-6-phosphate dehydrogenase in the rat liver].
A decrease in activity of glucose-6-phosphate dehydrogenase (G6PD) in Wistar rat liver tissue, caused by stress, was realized via a mechanism, involving adrenaline and hydrocortisone as first messengers and cAMP as a second messenger. In these systems G6PD II and IV isoenzymes were mainly inhibited. These are the urgent mechanisms for regulation of the enzymatic activity. Activation of G6PD within the reparative period after the stress was due to induction of the enzyme synthesis. Cooperative action of adrenaline, hydrocortisone and high density lipoproteins from blood serum, modulating the effect of adaptive hormones, was responsible for the enzyme induction. Actinomycin D and vinblastine (inhibitor of the cell microtubular apparatus) prevented the stimulation of G6PD isoenzymes caused by the cooperative hormonal effect. The data obtained suggest that the cooperative effect occurred as a result of lysosomes translocation to nucleus and the subsequent activation of chromatin.
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000097:blood* / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D005082:Physical Exertion; D051381:Rats; D011919:Rats, Inbred Strains; D014747:Vinblastine / Q000494:pharmacology
D007527:Isoenzymes; D008075:Lipoproteins, HDL; D003609:Dactinomycin; D014747:Vinblastine; D005954:Glucosephosphate Dehydrogenase; D006854:Hydrocortisone; D004837:Epinephrine
Vliianie adrenalina, gidrokortizona i LPVP syvorotki krovi na aktivnost' mnozhestvennykh form gliukozo-6-fosfatdegidrogenazy v pecheni krys.