[Direct determination of diethylstilbestrol and its monoconjugates in plasma].
Direct Determination of Diethylstilbestrol and its Monoconjugates in Plasma After "homogeneous ion pair extraction" with CH3OH from plasma of patients suffering from metastatic prostate cancer and subsequent partitioning on a C18-phase diethylstilbestrol (E-DES) and its monoconjugates (glucuronide, sulfate) as well as its glucuronide-sulfate conjugate may be detected quantitatively by HPLC/UV (236 nm) or HPLC/ED (+1.0 V). Working electrode is a special home-made carbon paste electrode. The range of detection is in the low ng range (2-5 ng/ml plasma); standard deviation is less than 5%. It is the first time that plasma levels of these metabolites of fosfestrol (Honvan) could be measured directly without prior hydrolysis.
D002853:Chromatography, Liquid; D004054:Diethylstilbestrol / Q000097:blood*; D005965:Glucuronates / Q000097:blood; D006801:Humans; D007700:Kinetics; D008297:Male; D011471:Prostatic Neoplasms / Q000097:blood; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000097:blood
D005965:Glucuronates; D013431:Sulfates; D004054:Diethylstilbestrol
Direkte Bestimmung von Diethylstilbestrol und seinen Monokonjugaten im Plasma.