title: Higher order structure of chromatin: evidence from photochemically detected linear dichroism.
abstract: We have used photochemically detected linear dichroism to measure the separate average angular orientations of nucleosomes and linker DNA in 30-nm chromatin fibers of varying linker size (20-80 base pairs). Our results indicate that the average tilt angles vary with linker size, but not in a monotonic manner, suggesting that the constancy of geometry of the 30-nm fiber is maintained by compensatory changes of nucleosomal tilt which accommodate packing of variable lengths of linker DNA. We discuss the compatibility of our results with the various classes of models that have been proposed for the 30-nm fiber, including the continuous solenoid model and models built from the basic unit of the zig-zag ribbon. Many models can be eliminated, and all have to be modified to fit our results for chromatins with very long linkers.
mesh_terms: D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000302:isolation & purification*; D004912:Erythrocytes / Q000032:analysis; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D008297:Male; D008836:Micrococcal Nuclease; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000648:ultrastructure; D010777:Photochemistry; D012617:Sea Urchins; D013045:Species Specificity; D013053:Spectrophotometry / Q000379:methods; D013094:Spermatozoa / Q000032:analysis
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease