title: Computer-aided three-dimensional reconstruction of nematode embryos from EM serial sections.
abstract: Embryos of the nematode Caenorhabditis elegans were serially sectioned and photographed in the electron microscope (EM). The micrographs were used to produce three-dimensional (3D) reconstructions. Size and position of each nucleus were entered into a computer, displayed as spheres, and were color-coded to indicate lineage membership. Location in space and position in the cell cycle are generally adequate criteria to identify cells. The reconstructions allow visualization of lineage-related topographic patterns and ultrastructural analysis of differently determined cells.
mesh_terms: D000818:Animals; D002107:Caenorhabditis / Q000196:embryology* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003201:Computers; D008854:Microscopy, Electron