Spontaneous and induced sister-chromatid exchanges in cultured mosquito cells.
Four mosquito cell lines derived from species of the genus Aedes were examined for spontaneous and induced sister-chromatid exchanges by the BrdU-labelling method. The five agents tested for SCE induction were: ethyl methanesulphonate (EMS), methyl methanesulphonate (MMS), ethyl nitrosourea (ENU), methyl nitrosourea (MNU), and 5-bromo-2-deoxyuridine (5-BrdU). It was found that there is significant interspecific and even intraspecific variation in the spontaneous frequency of SCEs. All five agents were effective inducers of SCEs and the induction was dose-dependent within the range of chemical concentrations used. This study shows that the mosquito cell lines may provide a simple and reliable in vitro assay system for testing the genotoxic effects of various agents, particularly on insects.
D000330:Aedes / Q000235:genetics*; D000818:Animals; D001973:Bromodeoxyuridine / Q000633:toxicity; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005038:Ethylnitrosourea / Q000633:toxicity; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008770:Methylnitrosourea / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013045:Species Specificity
D009153:Mutagens; D008770:Methylnitrosourea; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; D001973:Bromodeoxyuridine; D005038:Ethylnitrosourea