Novel neutrophil chemotactic factor derived from human peripheral blood mononuclear leucocytes.
Human mononuclear leucocytes isolated from the peripheral blood by centrifugation on Ficoll-Hypaque cushions and adherent on plastic petri dishes, produced a chemotactic factor that attracted human neutrophilic granulocytes to the same extent as did optimal concentrations of the complement split product C5a and the leukotriene B4. The active component eluted from a Sephadex G-50 gel filtration column as a single peak with an apparent molecular weight of 10,000. The chemotactic activity was resistant to reductive cleavage of disulfide bonds and heating at 100 degrees C for 30 min but was lost when reduction and heating were combined. Digestion with a proteolytic enzyme eliminated the attractive potential. The data suggest that this is a novel chemotactic peptide. It is conceivable that it has been seen previously and was mistaken for a lymphokine or interleukin 1.
D002630:Chemotactic Factors / Q000032:analysis*; D002634:Chemotaxis, Leukocyte; D003513:Cycloheximide / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D008970:Molecular Weight; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D009504:Neutrophils / Q000032:analysis*; D011402:Pronase / Q000378:metabolism; D013997:Time Factors
D002630:Chemotactic Factors; D003513:Cycloheximide; D011402:Pronase