title: Regulation of nitrile-hydratase synthesis in a Brevibacterium species.
abstract: The regulation of nitrile-hydratase biosynthese was studied in Brevibacterium sp R 312. Enzyme biosynthesis was not influenced by any carbon and nitrogen source used in the growth medium. It was, however, repressed by amide and amide analogues. Acetamide repressed nitrile-hydratase biosynthesis and induced the wide-spectrum amidase. Therefore, it appeared reasonable to hypothesize a single repressor gene for the nitrile-hydratase/wide-spectrum amidase system.
mesh_terms: D000081:Acetamides / Q000378:metabolism; D000577:Amides / Q000378:metabolism; D000645:Ammonium Sulfate / Q000378:metabolism; D001951:Brevibacterium / Q000201:enzymology* / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D006836:Hydro-Lyases / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation
chemical_list: D000081:Acetamides; D000577:Amides; D002244:Carbon; D006836:Hydro-Lyases; C048831:nitrile hydratase; D005947:Glucose; D000645:Ammonium Sulfate