A highly sensitive assay method for human placental alkaline phosphatase involving a monoclonal antibody bound to a paper disk.
A monoclonal antibody which is specific for human placental alkaline phosphatase and does not cross-react at all with intestinal alkaline phosphatase was prepared, and a procedure for the determination of placental alkaline phosphatase activity in serum was developed involving this monoclonal antibody bound to a paper disk. The minimum amount of placental alkaline phosphatase detectable by this method is 0.0025 King-Armstrong unit. Good correlation with the heat-treatment method was obtained. Therefore this proposed method can be used as a routine clinical test for the determination of serum placental alkaline phosphatase.
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000915:Antibody Affinity; D004589:Electrophoresis, Disc; D004592:Electrophoresis, Starch Gel; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007700:Kinetics; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase