Long-term cultures of human marrow cells.
We review the technical problems and examine the different parameters concerning the culture of human bone marrow over a long period of time (10-20 weeks). The main points of interest for the study of normal hematopoiesis and its impairment in blood diseases are indicated. The aim of this review is practical as well as theoretical.
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004900:Erythroblasts / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism; D006098:Granulocytes / Q000502:physiology; D006402:Hematologic Diseases / Q000503:physiopathology; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D051379:Mice; D009196:Myeloproliferative Disorders / Q000503:physiopathology; D013234:Stem Cells / Q000502:physiology
D005353:Fibronectins; D003094:Collagen