[Establishment of the human stomach poorly-differentiated adenocarcinoma (PdGC-81) cell line and its biological character].
The gastric cancer specimen was obtained from a female patient, 65 years old. The metastatic node in the greater omentum, demonstrated by pathology as a poorly-differentiated adenocarcinoma, was cut into small cubes (1 mm3) and cultured. The human stomach poorly-differentiated adenocarcinoma cell line (PdGC-81) was thus established. It had been transferred for 83 generations during 13 months. This cell line showed the following characters: 1. The pathological features of the tumor transplanted in mice was similar to that of the original inoculated lesion. 2. Microscopically, the majority of the cells were elliptical and polygonal epithelial cells with abnormal mitotic figures and 2-4 nucleoli. 3. Electronmicroscopically, there were rich microvilli on the surface of the cells. Desmosomes and abundant ribosomes were also observed. 4. The growth curves of the 33rd and 65th passages were determined. They multiplied 23 and 21 times within 8 days, the doubling times were 41.4 and 43.4 hours. The mitotic indexes attained their peak on day 3 in 33rd passage (80.5%) and on day 4 in 65th passage (72%). 5. The numbers of the chromosomes ranged from 56 to 60. This cell line may be considered as the subtriploid.
D000230:Adenocarcinoma / Q000473:pathology* / Q000648:ultrastructure; D000368:Aged; D002460:Cell Line; D005260:Female; D006801:Humans; D008940:Mitotic Index; D011123:Polyploidy; D013274:Stomach Neoplasms / Q000473:pathology* / Q000648:ultrastructure