Biosynthesis of a novel prostaglandin, delta 17-PGE1, in the ram.
The prostaglandin, delta 17-PGE1, was purified from extracts of ram seminal fluid by reversed phase high performance liquid chromatography (HPLC) and identified after conversion to delta 17-PGB1 by UV-analysis and by capillary column gas chromatography-mass spectrometry (GC-MS). It was also formed by incubation of a homogenate of ram vesicular glands. The amount of delta 17-PGE1 in the seminal fluid and in the homogenate averaged 12% and 25% of the amount of PGE2, respectively. The results show that cis-8,11,14,17-eicosatetraenoic acid can be metabolized to prostaglandins in vivo in the ram.
D000527:Alprostadil / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D012661:Semen / Q000032:analysis; D012669:Seminal Vesicles / Q000378:metabolism; D012756:Sheep
C033246:prostaglandin E3; D000527:Alprostadil