Detection of viral antigens by solid phase radioimmunoassay on polyethylene film.
Polyethylene film, without any pretreatment, may serve as a solid phase (SP) for RIA. Viral antigens (HBsAg, and influenza virus) are detected by SP-RIA on the film with a sensitivity of about 2-3 ng/ml or 40-60 pg/assay. The use of polyethylene film allows one to record RIA autographically. The use of micro amounts of reagents and specimens tested is an added advantage. No special equipment is necessary, the method is inexpensive, easy to perform and may be used for mass screening.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001345:Autoradiography; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D009975:Orthomyxoviridae / Q000302:isolation & purification; D011095:Polyethylenes; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats
D000956:Antigens, Viral; D006514:Hepatitis B Surface Antigens; D011095:Polyethylenes