Two RNA species co-purify with RNase P from the fission yeast Schizosaccharomyces pombe.
RNase P activity from Schizosaccharomyces pombe co-purifies with two RNA species. These RNAs are associated with enzyme activity as judged by titrated micrococcal nuclease inactivation experiments. The two RNAs, K1- and K2-RNA, are 285 and 270 nucleotides long, respectively. Both RNAs are transcribed from one gene, present in a single copy in the haploid genome. The primary and a secondary structure of K RNAs have been determined and compared with M1 RNA, their counterpart from Escherichia coli. Very limited sequence homology was observed, and this agrees with the finding that no cross-hybridization with M1 RNA can be detected in a Southern analysis with yeast genomic DNA. However, the secondary structures of K RNA and M1 RNA show the same basic organization and one conserved local motif, the sequence GUG--AGGPu in an exposed hairpin loop.
D001483:Base Sequence; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D005796:Genes; D005800:Genes, Fungal; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012331:RNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D043262:Ribonuclease P; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins; D012331:RNA, Fungal; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli