Immunological protection of rabbits infected with Staphylococcus aureus isolates from patients with toxic shock syndrome.
Toxic shock syndrome toxin-1 (TSST-1) isolated from the growth medium of Staphylococcus aureus 1169 and 555 was used to immunize male rabbits before infection with either a TSST-1+ or a TSST-1- strain of S. aureus isolated from cases of TSS. None of the immunized rabbits died as a result of the infections, whereas 50% of the nonimmunized rabbits infected with the TSST-1- strain, D4508, and 75% of those infected with the TSST-1+ strain, 555, died. Western blots of crude extracellular protein preparations probed with sera from immunized rabbits indicated that the TSST-1- strain produces a 30,000-molecular-weight protein that cross-reacts with antiserum to TSST-1. Because both organisms caused similar diseases in rabbits, we propose to designate the cross-reacting protein as TSST-2.
D000293:Adolescent; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology; D001427:Bacterial Toxins; D003429:Cross Reactions; D004768:Enterotoxins / Q000276:immunology*; D005260:Female; D006801:Humans; D007114:Immunization; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D012772:Shock, Septic / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000032:analysis / Q000276:immunology; D018089:Superantigens
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal