Somatomedin C/insulin-like growth factor I: simplified purification procedure and biological activities of the purified growth factor.
Human somatomedin C has been purified from Cohn fraction IV paste by a simplified procedure using chromatofocusing, hydroxylapatite chromatography and reverse-phase high performance chromatography. The purified material has a specific activity by somatomedin C radioimmunoassay of 9160 units/mg (1 unit is defined as the amount of somatomedin present in 1 ml normal adult male human serum), representing a 650,000-fold purification, and possesses sulphation, mitogenic and insulin-like activities (specific activities of 3388 units/mg, 832 units insulin equivalents/mg and 1122 units/mg respectively). Somatomedin C is shown to be a potent stimulator of DNA synthesis (50% maximum stimulation at 150 fmol/ml) in isolated chondrocytes derived from costal cartilage, a major physiological target tissue.
D000596:Amino Acids / Q000032:analysis; D001682:Biological Availability; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007334:Insulin-Like Growth Factor I / Q000302:isolation & purification*; D011863:Radioimmunoassay; D013002:Somatomedins / Q000302:isolation & purification*
D000596:Amino Acids; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I