Determination of factor X activator in the venom of the saw-scaled viper (Echis carinatus).
Factor X activator in Echis carinatus venom was determined by incubating the zymogen 'factor X' with venom, interrupting the activation process by ethylenediaminetetraacetic acid and measuring the generated proteinase 'factor Xa' by means of a synthetic chromogenic substrate. A comparison of factor X- and prothrombin-activating potencies in E. carinatus venoms of five different geographic origins revealed no correlation between these two procoagulant activities.
D005170:Factor X / Q000032:analysis*; D015951:Factor Xa; D011516:Prothrombin / Q000032:analysis; D013997:Time Factors; D014757:Viper Venoms / Q000032:analysis* / Q000494:pharmacology
D014757:Viper Venoms; D011516:Prothrombin; D005170:Factor X; D015951:Factor Xa