Effects of di-(2-ethylhexyl) phthalate and five of its metabolites on rat testis in vivo and in in vitro.
In an attempt to establish which compound or compounds are responsible for the testicular damage observed after administration of di-(2-ethylhexyl) phthalate (DEHP) in rats, the effects of the parent compound and five of its major metabolites (mono-(2-ethylhexyl) phthalate (MEHP), 2-ethylhexanol (2-EH), mono-(5-carboxy-2-ethylpentyl) phthalate, mono-(2-ethyl-5-oxohexyl) phthalate and mono-(2-ethyl-5-hydroxyhexyl) phthalate) were investigated in vivo and in vitro. The concentrations of MEHP and the three MEHP-derived metabolites in plasma were determined after single and multiple oral doses of DEHP. The plasma concentrations and areas under the plasma concentration-time curves (AUC's) of each of the MEHP-derived metabolites were considerably lower than those of MEHP both after single and after repeated administration of 2.7 mmol of DEHP/kg body weight. The mean elimination half-life of MEHP was significantly shorter in animals given repetitive doses than in those given a single dose, but there was no statistically significant difference between the mean AUC values. No testicular damage was observed in young rats given oral doses of 2.7 mmol of DEHP or 2-EH/kg body weight daily for five days. In animals which received corresponding doses of MEHP the number of degenerated spermatocytes and spermatids was increased, whereas no such effects were found in animals given the MEHP-derived metabolites. MEHP was also the only compound that enhanced germ cell detachment from mixed primary cultures of Sertoli and germ cells.
D000818:Animals; D001711:Biotransformation; D001835:Body Weight / Q000187:drug effects; D002478:Cells, Cultured; D004051:Diethylhexyl Phthalate / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D008657:Metabolic Clearance Rate; D009929:Organ Size / Q000187:drug effects; D010795:Phthalic Acids / Q000378:metabolism / Q000494:pharmacology*; D010968:Plasticizers / Q000378:metabolism / Q000494:pharmacology*; D011467:Prostate / Q000033:anatomy & histology / Q000187:drug effects; D051381:Rats; D013090:Spermatocytes / Q000187:drug effects; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects*
D010795:Phthalic Acids; D010968:Plasticizers; D004051:Diethylhexyl Phthalate