[Sensitivity to benzo(a)pyrene of epithelial and mesenchymal cells of embryonic lungs of mice susceptible (line A) and resistant (line C57BL) to lung blastomogenesis].
Proliferative activity of alveolar (EA) and bronchial (EB) epithelial cells, mesenchymal cells of explants (ME) and mesenchymal cells migrated on the cellulose filter (MF) was studied autoradiographically in the normal cultures and after treatment with benz(a)pyrene (BP). Labeling index (LI) of cells from the treated explants was higher or lower than in normal cultures, or did not differ from normal values. The effect of the treatment depended on BP dose, mouse strain, and the type of cells. Epithelial cells, especially EB, and ME cells from embryonic lungs of A mice were very sensitive to growth-stimulating effect of BP. In treated cultures from C57BL mice LI increased only in EB cells, however, unlike A mice, the effect inversely correlated with BP doses. Proliferative activity of EA, ME and MF cells was lower than in untreated explants from C57BL, the effect of treatment increasing with higher BP doses. The importance of local cell-tissue factors, namely epithelial-mesenchymal interactions, and the role of lung mesenchyma in the realization of genetically determined sensitivity to spontaneous and induced lung blastomogenesis in C57BL and A mice are discussed.
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004847:Epithelial Cells; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D051379:Mice; D008805:Mice, Inbred A / Q000235:genetics; D008810:Mice, Inbred C57BL / Q000235:genetics; D009924:Organ Culture Techniques
D001564:Benzo(a)pyrene
Chuvstvitel'nost' k benz(a)pirenu kletok épiteliia i mezenkhimy émbrional'nykh legkikh mysheĭ, predraspolozhennykh (liniia A) i ustoĭchivykh (liniia C57BL) k blastomogenezu legkikh.