[Induction of cytochrome P-450 and b5 in hepatocyte subpopulations with phenobarbital].
Hepatocytes isolated from intact and phenobarbital-treated rats were separated by Ficoll discontinuous density gradient into five subpopulations. About 85% of the total cell number sedimented within the range of 1.044 to 1.126 g X cm-3. In intact rats, heavy hepatocytes contained about twice as much cytochrome P-450 and about 3 times as much cytochrome b5 as light cell population. Phenobarbital induced cytochrome P-450 in all subpopulations, with a more pronounced increase observed in light hepatocytes. On the contrary, maximum induction of cytochrome b5 was noted in heavy hepatocytes. The results demonstrate the absence of uniform distribution of cytochrome P-450 and b5 in hepatocyte subpopulations. The highest concentrations of both cytochromes are observed in heavy (centrolobular) hepatocytes.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D003573:Cytochrome b Group / Q000096:biosynthesis*; D015786:Cytochromes b5; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003573:Cytochrome b Group; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital
Induktsiia tsitokhromov P-450 i b5 v subpopuliatsiiakh gepatotsitov fenobarbitalom.