Detrimental effect of hypophosphatemia on the severity and progression of ischemic acute renal failure.
Phosphate depletion may prevent progression of experimental chronic renal failure. The present study was designed to examine the effect of phosphate depletion on the severity and progression of ischemic acute renal failure. Two groups of rats, phosphate-depleted (PD) and phosphate-repleted (PR), were pair-fed a regular diet for 10 days; the diet of the PD rats also contained 15 g% dihydroxyaluminum aminoacetate. After 10 days, serum phosphate in PD rats averaged 3.9 +/- 0.7 mg%, compared to 6.1 +/- 0.6 mg% in the PR rats (p less than 0.001). The weight gain during the equilibration period was comparable in both groups (24.2 +/- 9.3 vs. 25.7 +/- 10.4 g; NS). Creatinine clearance, hematocrit, renal cortical adenine nucleotides, tissue calcium, mitochondrial calcium and mitochondrial respiration were similar in both groups after 10 days of the diet. A 50-min bilateral clamping of both renal arteries and veins was then undertaken in both groups. The PD rats demonstrated more severe acute renal failure at 24 h after the ischemic insult. Serum creatinine and creatinine clearance for the PD and PR rats were 4.0 +/- 0.6 versus 3.1 +/- 0.9 mg% (p less than 0.005) and 7.8 +/- 8.1 versus 85.3 +/- 70.7 microliter/min (p less than 0.001), respectively. Tissue calcium (131.3 +/- 103.0 vs. 63.5 +/- 36.0 mmol/kg dry weight; p less than 0.02) and mitochondrial calcium (49.4 +/- 24.0 vs. 29.5 +/- 14.6 nmol/mg prot.; p less than 0.02) were significantly higher in the PD rats.(ABSTRACT TRUNCATED AT 250 WORDS)
D058186:Acute Kidney Injury / Q000378:metabolism / Q000503:physiopathology*; D000284:Administration, Oral; D000536:Aluminum Hydroxide / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001835:Body Weight; D004032:Diet; D005998:Glycine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D007511:Ischemia / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D010710:Phosphates / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D010710:Phosphates; D000536:Aluminum Hydroxide; C007420:dihydroxyaluminum aminoacetate; D005998:Glycine