Intracellular responses of raphe magnus neurons during the jaw-opening reflex evoked by tooth pulp stimulation.
Neurons in the nucleus raphe magnus (RM) may play an important role in the modulation of nociception. To determine how RM neurons are activated during a nociceptive reflex, the intracellular responses of raphe neurons were studied during the jaw-opening reflex (JOR) elicited by tooth pulp shock in lightly anesthetized cats. Tooth pulp stimulation produces reflex EMG activation of the digastric muscle at a latency of 7-11 ms, resulting in jaw opening. Tooth pulp shock that elicits the JOR also produces an EPSP in a subset of raphe neurons. This EPSP consists of an early small depolarization that occurs at a latency of 10-15 ms followed by a larger depolarization at a latency of 20-60 ms. In all cases the latency to EPSP is longer than the latency to digastric EMG onset. Electrical stimulation of the 4 paws elicits oligosynaptic EPSPs in the same cells at a latency of 16-20 ms. Electrical train stimulation of the midbrain periaqueductal gray region (PAG) suppresses the JOR. Single shock stimulation at the same PAG sites that suppress the JOR evokes monosynaptic EPSPs in the large majority of raphe neurons recorded. In all cases, the threshold for EPSP is below the threshold for suppression of the JOR. The EPSP amplitude is a direct function of PAG stimulus intensity and there is temporal summation of EPSPs evoked by paired PAG shocks. At condition-test intervals of 40-90 ms, train stimulation of PAG suppresses the tooth pulp-evoked EPSP in raphe neurons. The threshold for EPSP suppression occurs at a PAG stimulation intensity below that required for suppression of the JOR. The present findings provide evidence that RM neurons may play an important role in the modulation of the tooth pulp-evoked JOR, but only after the initial withdrawal reflex has occurred.
D000818:Animals; D001931:Brain Mapping; D002415:Cats; D003782:Dental Pulp / Q000294:innervation*; D004558:Electric Stimulation; D004576:Electromyography; D004597:Electroshock; D005071:Evoked Potentials; D007568:Jaw / Q000294:innervation*; D009474:Neurons / Q000502:physiology*; D010487:Periaqueductal Gray / Q000502:physiology; D011903:Raphe Nuclei / Q000166:cytology / Q000502:physiology*; D012018:Reflex / Q000502:physiology*; D012154:Reticular Formation / Q000502:physiology; D013788:Thalamus / Q000502:physiology