Selective retention of organic phosphate esters and phosphonates on aluminium oxide.
Compounds containing the -PO3H2 function, such as monoesters of phosphoric acid and phosphonic acids, specifically bind to aluminium oxide in aqueous solution under experimental conditions where non-phosphorylated compounds are completely desorbed. The bound organic phosphate can be specifically displaced by aqueous solution of inorganic phosphates thus allowing their separation or detection by a technique similar to that of affinity chromatography. The consequences of this finding for phosphate compound biochemistry are discussed.
D000535:Aluminum; D000537:Aluminum Oxide; D002845:Chromatography; D063065:Organophosphonates / Q000302:isolation & purification*; D009943:Organophosphorus Compounds / Q000302:isolation & purification*
D063065:Organophosphonates; D009943:Organophosphorus Compounds; D000535:Aluminum; D000537:Aluminum Oxide