A comparative study of buffer systems as cryoprotectants for human spermatozoa.
Several cryoprotective media currently in use for human sperm cryopreservation have been directly compared with each other by use of a static vapor freezing technique. The TEST-citrate-yolk buffers with glycerol resulted in the highest motility and longevity after thawing; thus, their use is recommended.
D000818:Animals; D002021:Buffers / Q000494:pharmacology*; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D003451:Cryoprotective Agents; D004530:Egg Yolk; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D006531:HEPES / Q000494:pharmacology; D006801:Humans; D006878:Hydroxides / Q000494:pharmacology; D007552:Isotonic Solutions / Q000494:pharmacology; D008297:Male; D008892:Milk; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D014325:Tromethamine / Q000031:analogs & derivatives / Q000494:pharmacology
D002021:Buffers; D002951:Citrates; D003451:Cryoprotective Agents; D006878:Hydroxides; D007552:Isotonic Solutions; D017680:Potassium Compounds; C037692:Tyrode's solution; D014325:Tromethamine; D019343:Citric Acid; C004551:TES; D005990:Glycerol; D011188:Potassium; D006531:HEPES; C029943:potassium hydroxide