[Cultivation of urologic and male genital tumor cells].
Thirty specimens from 30 patients with urologic and male genital system tumors were cultured. Eighteen (60%) showed initial cell growth. Four (13%) were successful in primary culture. In a renal cell carcinoma and a bladder transitional cell carcinoma, the subcultures were successful after the primary culture. In eight months, having transferred the former for twelve generations and the latter for twenty-seven generations, these two cell lines are established. The morphological characters of the cultured cells, eradication of fibroblasts, conditions of culture and prevention of contamination are discussed.
D002292:Carcinoma, Renal Cell / Q000473:pathology; D002295:Carcinoma, Transitional Cell / Q000473:pathology; D002460:Cell Line; D002478:Cells, Cultured; D005834:Genital Neoplasms, Male / Q000473:pathology*; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology; D008297:Male; D001749:Urinary Bladder Neoplasms / Q000473:pathology; D014571:Urologic Neoplasms / Q000473:pathology*