Purification and characterization of chymodenin. A hormone-like peptide from porcine duodenum.
Chymodenin, a hormone-like duodenal peptide which rapidly alters the proportions of secreted pancreatic digestive enzymes to a mixture relatively richer in chymotrypsinogen than that found in basal secretion, has been purified to homogeneity. The starting material was an acidic methanol-soluble, neutral pH-insoluble fraction of an acetic acid extract of porcine duodenum; the purification consisted of cation-exchange chromatography on SP-Sephadex and CM-Sephadex in ammonium bicarbonate gradients, and gel filtration on Sephadex G-75 in dilute acetic acid. The yield of material was followed by radioimmunoassay. Homogeneity was determined from chymodenin's behavior in disc gel electrophoresis in an acidic counter-migration-of-dye system, sodium dodecyl sulfate-urea gels, gel filtration, dansyl-Edman reaction, reversed-phase high pressure liquid chromatography, isotachophoresis, and sedimentation equilibrium ultracentrifugation. The electrophoretic mobility, the molecular weight of 9,000-10,000, and the biological activity differed from those of other gastrointestinal peptide hormones. The amino acid composition was unique. Chymodenin is the first purified hormone-like substance reported capable of altering the composition of the mixture of secreted digestive enzymes, independent of the stimulation of massive pancreatic protein output.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004386:Duodenum / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005768:Gastrointestinal Hormones / Q000302:isolation & purification*; D036341:Intercellular Signaling Peptides and Proteins; D010189:Pancreatic Juice / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine
D000596:Amino Acids; D005768:Gastrointestinal Hormones; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C007058:chymodenin