Antitumor polysaccharide-induced tumor-regressing factor in the serum of tumor-bearing mice: purification and characterization.
Marked tumor-regressing activity was induced in the serum of S180 tumor-bearing mice by injection of an antitumor polysaccharide, CM-TAK [carboxymethylated beta(1-3)glucan]. Maximal activity was induced 7-14 days after the tumor transplantation and 10-12 h after CM-TAK treatment. A quantitative assay for the activity was established on the basis of the initial decrease in the number of the tumor cells within 24 h. The factor with tumor-regressing activity was purified 10,000-fold by the series of hydroxylapatite chromatography, ammonium sulfate precipitation, anion-exchange chromatography, gel filtration, and boronate-mediated affinity chromatography. The molecular weight was estimated to be 250,000 by gel filtration. The activity was proteinase K sensitive, but relatively resistant to trypsin. Neuraminidase did not affect the activity. It is believed that the tumor-regressing factor is different from the tumor necrosis factor.
D000421:Alcaligenes; D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood / Q000302:isolation & purification; D005936:Glucans / Q000494:pharmacology*; D006358:Hot Temperature; D051379:Mice; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D011506:Proteins / Q000302:isolation & purification; D012513:Sarcoma, Experimental / Q000628:therapy*; D013997:Time Factors
D000970:Antineoplastic Agents; D005936:Glucans; D011135:Polysaccharides, Bacterial; D011506:Proteins