Bulk growth procedures and a button agglutination test for Campylobacter.
Bulk-cell yields were obtained from 4 Campylobacter spp incubated aerobically without the use of a special atmosphere. A button agglutination test was developed for quantitation of blood serum antibodies against C fetus subsp venerealis, C fetus subsp fetus, C jejuni, and "C hyointestinalis." The test was sensitive, and different individuals reading it usually attained the same titers. Cells of C fetus subsp venerealis, C fetus subsp fetus, and "C hyointestinalis" grown aerobically in broth made satisfactory antigens for the button test, but some cell pools of C jejuni had a tendency to autoagglutinate. Cells of C jejuni grown on blood agar had less tendency to autoagglutinate.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002167:Campylobacter / Q000254:growth & development* / Q000276:immunology; D002169:Campylobacter Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D003470:Culture Media; D007106:Immune Sera
D000907:Antibodies, Bacterial; D003470:Culture Media; D007106:Immune Sera