Monoclonal antibodies directed against the flagella of Campylobacter jejuni: cross-reacting and serotypic specificity and potential use in diagnosis.
Nine monoclonal antibodies directed against the flagella of Campylobacter jejuni strain 81116 have been investigated for serotypic and cross-reacting activity using a panel of 17 Penner serotype strains of C. jejuni. Four monoclonal antibodies were exclusively specific for serotype-6 strains, which was the serotype of C. jejuni strain 81116. Two monoclonal antibodies cross-reacted with all the flagellated strains of C. jejuni tested. One of these cross-reacting monoclonal antibodies, CF5, was found to react with all other Campylobacter species except C. sputorum bulbulus but it did not react with other bacterial enteropathogens. An antigen-capture ELISA technique was established, using this monoclonal antibody, which could detect flagellar antigen in human faecal material. These anti-flagella monoclonal antibodies therefore may be valuable in the diagnosis and serotyping of C. jejuni in clinical material.
D000818:Animals; D000911:Antibodies, Monoclonal; D002169:Campylobacter Infections / Q000175:diagnosis; D002168:Campylobacter fetus / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005407:Flagella / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012703:Serotyping
D000911:Antibodies, Monoclonal