Metabolic turnover of proliferation-related nuclear proteins in serum-stimulated Swiss mouse 3T3 cells.
Stimulation of quiescent Swiss mouse 3T3 cells either by serum or by pure growth factors induces DNA synthesis after a lag period of about 15 h. Following restimulation by serum or by growth factors there is an overall increase of 2-4-fold in the rate of biosynthesis of nuclear proteins. Two nuclear polypeptides show specific temporal correlations with the transition from quiescence to proliferation. The synthesis of p30 (30 kDa, pI 5.2) is at a maximum within 5 h of restimulation, while the synthesis of p36 (36 kDa, pI 4.25) is first seen at 10-12 h after restimulation. The synthesis of p36 correlates well with the initiation of DNA biosynthesis. The metabolic turnover of both of these proteins has been estimated by pulse-chase and by cycloheximide inhibition experiments. They both have a half-life of 10-15 h and appear to be cell-cycle related.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005312:Fetal Blood / Q000502:physiology*; D006207:Half-Life; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009698:Nucleoproteins / Q000096:biosynthesis / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000378:metabolism
D003470:Culture Media; D009698:Nucleoproteins; D004247:DNA; D003513:Cycloheximide; D009955:Ornithine Decarboxylase