title: Replication of the B19 parvovirus in human bone marrow cell cultures.
abstract: The B19 parvovirus is responsible for at least three human diseases. The virus was successfully propagated in suspension cultures of human erythroid bone marrow from patients with hemolytic anemias; release of newly synthesized virus into the supernatants of infected cultures was observed. This culture system allowed study at a molecular level of events associated with the B19 life cycle. The B19 parvovirus replicated through high molecular weight intermediate forms, linked through a terminal hairpin structure. B19 replication in vitro was highly dependent on the erythropoietic content of cultures and on addition of the hormone erythropoietin.
mesh_terms: D000743:Anemia, Hemolytic / Q000382:microbiology*; D001853:Bone Marrow / Q000382:microbiology*; D002478:Cells, Cultured; D003470:Culture Media; D004279:DNA, Viral / Q000032:analysis; D004921:Erythropoietin / Q000378:metabolism; D006801:Humans; D010321:Parvoviridae / Q000254:growth & development*; D014779:Virus Replication
chemical_list: D003470:Culture Media; D004279:DNA, Viral; D004921:Erythropoietin