title: The in vitro penetration and distribution of T-2 toxin through human skin.
abstract: The penetration and distribution of T-2 toxin in excised human abdominal skin has been determined for a dose range of 1.0-2.6 micrograms/cm2 skin using an ethanol vehicle and a saline receptor solution. In all cases the overall percentage penetration of T-2 after 48 h was low, the greatest amounts of toxin being present in the stratum corneum with less in the epidermis and relatively little in the dermis. Vehicle: skin partition coefficients support this finding. Neither penetration nor distribution were changed by a rabbit serum receptor solution. Electron micrographs showed that at 1.8 micrograms/cm2 and above the contents of the intercellular space are leached out to leave the integument as a porous membrane. The distribution of T-2 within the skin after 48 h would suggest that for doses up to 2.6 micrograms/cm2 the irritative and inflammatory effects on the skin would be of more immediate concern than would systemic toxicity.
mesh_terms: D004058:Diffusion; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D010539:Permeability; D012717:Sesquiterpenes / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000648:ultrastructure; D012869:Skin Absorption; D012995:Solubility; D013605:T-2 Toxin / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D012717:Sesquiterpenes; D013605:T-2 Toxin