Participation of the microsomal CDP-diglycerides in the mitochondrial biosynthesis of phosphatidylglycerol.
Participation of microsomal CDP-diglycerides in mitochondrial biosynthesis of phosphatidylglycerol was studied by [3H]palmitoyl, [14C]linoleoyl, and [14C]arachidonoyl CDP-diglycerides and [3H]CDP-diglycerides which were bound to microsomal membranes, incubated with unlabelled mitochondrial membranes, and further incubated in the presence of radioactive sn-glycero-3-phosphate under conditions required for mitochondrial phosphatidylglycerol biosynthesis. Ten to 15% of microsomal radioactive CDP-diglycerides was transferred to mitochondrial membranes and incorporated into mitochondrial radioactive lipids identified as phosphatidylglycerol, phosphatidylglycerophosphate, and, when [14C]linoleoyl CDP-diglycerides were used, diphosphatidylglycerol (cardiolipin).
D000818:Animals; D002250:Carbon Radioisotopes; D003567:Cytidine Diphosphate Diglycerides / Q000378:metabolism*; D006168:Guinea Pigs; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008862:Microsomes, Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000096:biosynthesis*; D014316:Tritium
D002250:Carbon Radioisotopes; D003567:Cytidine Diphosphate Diglycerides; D009702:Nucleoside Diphosphate Sugars; D010715:Phosphatidylglycerols; D014316:Tritium