title: FMRFamide- and pancreatic polypeptide-like immunoreactivity of endocrine cells in the midgut of a mosquito.
abstract: Immunocytochemical surveys of midguts from female mosquitoes, Aedes aegypti, reveal that half of the estimated 500 endocrine cells in a midgut contain a substance recognized by antisera to bovine pancreatic polypeptide and a molluscan peptide, FMRFamide (phenylalanine-methionine-arginine-phenylalanine-amide). With light microscopy the cells resemble an endocrine type because of their basal position in the epithelium, conical shape, and, in some instances, apical extensions to the lumen. At the ultrastructural level, the immunoreactive substance is contained specifically within the secretory granules of such cells. Immunoreactive cells are distributed exclusively in the midgut region where blood is stored, and ingestion of vertebrate blood reduces the number of such cells and the intensity of reaction in others. These two facts suggest that a blood meal stimulates release of the immunoreactive substance from the cells. Since the immunocytochemical localization is supplemented by a demonstrated secretory response, the cells are considered to be peptidergic endocrine cells.
mesh_terms: D000330:Aedes / Q000166:cytology* / Q000648:ultrastructure; D000818:Animals; D003429:Cross Reactions; D004064:Digestive System / Q000032:analysis / Q000166:cytology / Q000648:ultrastructure; D019835:FMRFamide; D005260:Female; D007118:Immunoassay; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009420:Nervous System / Q000032:analysis; D009842:Oligopeptides / Q000032:analysis*; D010179:Pancreas / Q000032:analysis; D010191:Pancreatic Polypeptide / Q000032:analysis; D051381:Rats
chemical_list: D009419:Nerve Tissue Proteins; D009842:Oligopeptides; D010191:Pancreatic Polypeptide; D019835:FMRFamide