Comparison of antipseudomonad activity of chlorine dioxide/chlorous acid-containing gel with commercially available antiseptics.
A chlorine dioxide-containing gel was compared with 3 commercially available antimicrobials and 1 antibiotic intended for topical use. This gel was tested at 0.5 X and 4 X and was found to be more effective as a 4 X gel in treating Pseudomonas aeruginosa-infected excised wounds on mice. To further compare this gel with other antiseptics, a murine bioassay was developed. This wound model consisted of an excised cutaneous wound on the dorsum of mice which were irradiated (800 rad) and inoculated with P aeruginosa at 10-fold dilutions, from 10(-2) to 10(-10). The wounds were observed for latency of infection or mice survival time as a function of concentration of viable organisms remaining after treatment. The advantage of this model was demonstrated where a standard curve based on latency did not consume as many test subjects and yet provided an estimate of viable organisms in each wound. In this model, the chlorine dioxide-containing gel was more active than were preparations of providone-iodine, chlorhexidene, or silver sulfadiazine and was similar to polymyxin-bacitracin-neomycin ointment as a topical antiseptic. The effectiveness of the tested gel was reduced if delays in treatment were longer than 1 hour.
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D001414:Bacitracin / Q000627:therapeutic use; D002710:Chlorhexidine / Q000627:therapeutic use; D002713:Chlorine / Q000008:administration & dosage / Q000627:therapeutic use*; D017606:Chlorine Compounds; D004338:Drug Combinations / Q000627:therapeutic use; D005782:Gels; D008297:Male; D051379:Mice; D009355:Neomycin / Q000627:therapeutic use; D010087:Oxides / Q000008:administration & dosage / Q000627:therapeutic use*; D011112:Polymyxin B / Q000627:therapeutic use; D011206:Povidone-Iodine / Q000627:therapeutic use; D011552:Pseudomonas Infections / Q000188:drug therapy*; D012837:Silver Sulfadiazine / Q000627:therapeutic use
D000891:Anti-Infective Agents, Local; D017606:Chlorine Compounds; D004338:Drug Combinations; D005782:Gels; D010087:Oxides; D001414:Bacitracin; D002713:Chlorine; C001816:bacitracin zinc, neomycin sulfate, polymyxin B, drug combination; C025109:chlorine dioxide; D011206:Povidone-Iodine; D009355:Neomycin; D011112:Polymyxin B; D002710:Chlorhexidine; D012837:Silver Sulfadiazine