title: Serum antibody titers in rats receiving repeated small subcutaneous injections of hemoglobin or polyhemoglobin: a preliminary report.
abstract: Cross-linking hemoglobin (Hb) into Polyhemoglobin (PolyHb) for use as an artificial blood substitute may affect its antigenicity. To investigate this, male Sprague-Dawley rats are immunized with one of the following: rat stroma-free Hb (rSFHb), rat PolyHb (rPolyHb), human stroma-free Hb (hSFHb), and human PolyHb (hPolyHb). Antibody titers are quantified using a double antibody radioimmunoassay. These results show that more antibodies are produced to hPolyHb than to hSFHb, whereas rSFHB and rPolyHb are relatively non-antigenic. Thus, under homologous conditions, cross-linking hemoglobin does not significantly increase its antigenicity, whereas under heterologous conditions the molecule becomes more antigenic.
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000276:immunology; D003429:Cross Reactions; D006454:Hemoglobins / Q000276:immunology*; D006801:Humans; D007114:Immunization; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; C037158:polyhemoglobin