Mechanisms controlling renal hemodynamics and electrolyte excretion during amino acids.
Our purpose was to investigate the mechanisms by which increased plasma amino acids elevate renal blood flow (RBF) and glomerular filtration rate (GFR). Since transport of amino acids and Na+ is linked in the proximal tubule, we hypothesized that increased amino acids might stimulate proximal tubular Na+ reabsorption (PRNa) and thus increase RBF and GFR by a macula densa feedback mechanism. A solution of four amino acids (Ala, Ser, Gly, Pro) was infused intravenously (0.075 mmol X kg-1 X min-1 total) into anesthetized dogs with normal kidneys (NK) and with kidneys in which the tubuloglomerular feedback mechanism was blunted by lowering renal artery pressure (LPK) or blocked by making the kidneys nonfiltering (NFK). In NK, RBF and GFR increased by 35 +/- 4% and 30 +/- 7% after 90 min of amino acid infusion, while PRNa (estimated from lithium clearance) and O2 consumption increased by 31 +/- 5% and 29 +/- 5% and distal Na+ delivery remained relatively constant. Autoregulation of RBF and GFR in response to step decreases in renal artery pressure was impaired during amino acids in NK. The hemodynamic responses to amino acids were abolished in LPK and NFK. Infusion of the nonmetabolized alpha-aminoisobutyric acid (0.075 mmol X kg-1 X min-1) into NK produced changes in renal hemodynamics that were similar to the responses observed with the four metabolizable amino acids. These data are consistent with the hypothesis that elevation of plasma amino acids increases RBF and GFR by a mechanism that requires an intact macula densa feedback. Metabolism of the amino acids does not appear to be necessary for these changes to occur.
D000596:Amino Acids / Q000494:pharmacology*; D000621:Aminoisobutyric Acids / Q000494:pharmacology; D000818:Animals; D001696:Biomechanical Phenomena; D004285:Dogs; D004573:Electrolytes / Q000652:urine*; D005919:Glomerular Filtration Rate; D006439:Hemodynamics / Q000187:drug effects; D006706:Homeostasis; D007263:Infusions, Parenteral; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D010477:Perfusion; D011312:Pressure; D012079:Renal Circulation / Q000187:drug effects*
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D004573:Electrolytes; C100049:2-aminoisobutyric acid