Subcellular and organ distribution of cholesterol epoxide hydrolase in the rat.
The subcellular and organ distributions of microsomal epoxide hydrolases measured with cis-stilbene oxide and cholesterol 5,6 alpha-epoxide as substrates have been investigated. These two enzyme activities were found to have essentially the same subcellular distribution, with the highest total and specific activities localized in rough and smooth endoplasmic reticulum. Among the tissues studied (i.e., liver, kidney, lung, testis, spleen, brain and intestinal epithelium), the highest specific activities were recovered in liver microsomes, where the activities were at least 5-fold greater than in any of the other microsomal preparations.
D000818:Animals; D002458:Cell Fractionation; D004721:Endoplasmic Reticulum / Q000201:enzymology; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors; D013045:Species Specificity; D013267:Stilbenes / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology*; D014018:Tissue Distribution
D013267:Stilbenes; D004851:Epoxide Hydrolases; C019885:cholesterol-5 alpha,6 alpha-epoxide hydrase; C025906:stilbene oxide