title: Low-molecular-weight proteinase inhibitor of human epidermis inhibiting papain and trypsin activity.
abstract: A low-molecular-weight proteinase inhibitor was isolated from human epidermal extracts by means of ultrafiltration, gel filtration and ion-exchange chromatography. On polyacrylamide gel electrophoresis (PAGE), the isolated fraction exhibited a single band of activity at pH 9.4, and inhibitory activity against papain and trypsin was detected in this band. Using sodium-dodecyl-sulphate PAGE, the molecular mass of the inhibitor was estimated to be 2,200 daltons.
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010206:Papain / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000302:isolation & purification*; D012867:Skin / Q000032:analysis*; D014361:Trypsin Inhibitors / Q000302:isolation & purification
chemical_list: D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D010206:Papain