Unique antigen of cultured Hodgkin's cells. A putative sialyltransferase.
Hodgkin's disease-derived giant cell lines (HD-cells) express high levels of ectosialyltransferase activity presumed to be a galactose-specific lectin recognizing the desialylated 3-fucosyl-N-acetyllactosamine structure (X-hapten). Both the anti-X-hapten monoclonal antibody VIM-D5 and a polyclonal antiserum to another galactose-lectin, the hepatic asialoglycoprotein receptor (HBP), recognize a 55,000-mol wt HD-cell protein (Paietta, E., R. J. Stockert, A. G. Morell, V. Diehl, and P. H. Weirnik. 1986. Proc. Natl. Acad. Sci. USA. 83:3451-3455.) That the expression of the 55,000-mol wt protein is restricted to HD-cells among X-hapten positive cells lines is confirmed in this study. The 55,000-mol wt protein is shown to be present on the cell surface and intracellularly, where an additional immunocrossreactive 150,000-mol wt protein is recognized. Extraction of the 55,000 mol wt protein from HD-cell lysates by affinity chromatography results in the loss of sialyltransferase activity. While evidence for a single protein possessing both the antigenic and the enzymatic activity is not direct, these results suggest that the ectosialyltransferase unique to HD-cells is a 55,000-mol wt membrane glycoprotein possessing the X-hapten oligosaccharide.
D000912:Antibodies, Neoplasm / Q000502:physiology; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D037263:Asialoglycoprotein Receptor; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D002451:Cell Compartmentation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006689:Hodgkin Disease / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D011233:Precipitin Tests; D012799:Sialyltransferases / Q000037:antagonists & inhibitors / Q000276:immunology*; D014166:Transferases / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D037263:Asialoglycoprotein Receptor; D002352:Carrier Proteins; D007106:Immune Sera; C027122:hepatic sialoglycoprotein receptor; D014166:Transferases; D012799:Sialyltransferases