Fine specificity and idiotype expression of anti-phosphorylcholine IgE and IgG antibodies.
The immune response to the phosphorylcholine (PC) hapten elicited in BALB/c mice by PC-keyhole limpet hemocyanin (KLH) is composed of 2 groups of antibodies with specificity to PC and phenyl-PC, respectively. They were designated as group I and group II anti-PC antibodies. In this report we demonstrate that anti-PC IgE antibodies elicited by PC-KLH or PC-ovalbumin belong to the group II and do not express the T15 idiotype. Anti-PC IgG1, IgG2a and IgG2b antibodies express group I characteristics in the primary response and bear the T15 idiotype. Later, after 5 weeks and 3 injections of PC-KLH or PC-ovalbumin, a change in these isotypes to group II antibodies is observed. In contrast, anti-PC IgE is a group II antibody throughout progression of the immune response. The regulation of group I and group II antibody expression in serum is independent of the genetic background of the animals.
D000818:Animals; D000918:Antibody Specificity; D002794:Choline / Q000031:analogs & derivatives*; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D010767:Phosphorylcholine / Q000276:immunology*
D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine; D007073:Immunoglobulin E; D010047:Ovalbumin; D006433:Hemocyanins; D002794:Choline