Radioimmunoassay for amatoxins by use of a rapid, 125I-tracer-based system.
In this new radioimmunoassay system for determination of amatoxins in urine and plasma, a novel chemical approach is used for antigen and 125I-tracer production, based on a detoxified alpha-amanitin derivative (aldoamanitin). Total assay time, including data processing, is less than 100 min. The lowest detectable concentration is 1 microgram/L for urine, 0.1 microgram/L for plasma. In the clinically significant range, within-run CVs are less than 8%. This new 125I-based assay is a significant improvement over existing 3H technology in terms of speed, precision, and freedom from interference.
D000546:Amanitins / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods; D013329:Structure-Activity Relationship
D000546:Amanitins; C018207:amatoxin; C049964:aldoamanitin