title: Synapse formation and induction of acetylcholine receptors by spinal neurones in cocultures with sympathetic ganglion and muscle cells.
abstract: Coculture of chick embryonic sympathetic neurones with spinal cord explants induced an age-dependent increase in the acetylcholine receptor numbers of the ganglion cells. These acetylcholine receptors did not appear to be necessary for the initial formation of spinal cord-ganglion synapses since their blockade with the alpha-bungarotoxin-horseradish peroxidase complex did not prevent synapse formation in culture. The presence of acetylcholine receptors appears to be sufficient for synapse formation since inappropriate motoneurone-ganglion synapses could form and were stable.
mesh_terms: D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002450:Cell Communication; D002642:Chick Embryo; D046508:Culture Techniques; D005728:Ganglia, Sympathetic / Q000502:physiology*; D008564:Membrane Potentials; D009132:Muscles / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000032:analysis / Q000096:biosynthesis*; D013116:Spinal Cord / Q000502:physiology*; D013569:Synapses / Q000502:physiology*
chemical_list: D002038:Bungarotoxins; D011950:Receptors, Cholinergic