One perfusion mixture for immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rat brain.
A perfusion mixture composed of glutaraldehyde and allyl-alcohol at pH 12 is compatible for the fixation and immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rodent central nervous system. A technical description of this procedure is provided to facilitate the replication of this immunocytochemical approach with these antibodies.
D000109:Acetylcholine / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D004298:Dopamine / Q000032:analysis*; D005404:Fixatives; D006651:Histocytochemistry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007120:Immunochemistry / Q000379:methods; D007202:Indicators and Reagents; D009638:Norepinephrine / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000032:analysis*
D005404:Fixatives; D007202:Indicators and Reagents; D012701:Serotonin; D000109:Acetylcholine; D004298:Dopamine; D009638:Norepinephrine