Role of phospholipids in activation of mitochondrial D(-)-beta-hydroxybutyrate dehydrogenase.
Although phosphatidylcholine (PC) has been shown to be the type of phospholipid required for activation of mitochondrial beta-hydroxybutyrate dehydrogenase (BDH), mixtures of phospholipids containing PC are more effective activators. This study shows that apo-BDH, purified from bovine-heart mitochondria, and phospholipid-reconstituted BDH appear to be polydisperse. Upon cross-linking with dimethylpimelimidate and acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), the enzyme exhibited molecular weight forms from monomeric to heptameric BDH as well as higher molecular weight aggregates that did not much penetrate the gels. When different phospholipid mixtures containing PC were used to activate apo-BDH, and the reconstituted samples were subjected to cross-linking and SDS-gel electrophoresis, a direct relationship was found between the activating effect of the phospholipids used and BDH monomer concentration in the gels. The effectiveness order of phospholipids used was as follows: a mixture of PC, phosphatidylethanolamine and diphosphatidylglycerol in a molar ratio of 5:4:1 greater than bovine-heart mitochondrial phospholipids greater than Asolectin greater than PC. These results suggest the following. In addition to PC, which is required by BDH, other types of phospholipids play a role in activation of purified apo-BDH, possibly via enzyme disaggregation. The activity exhibited by purified, phospholipid-reconstituted BDH is associated mainly with the lower molecular aggregates of the enzyme, especially monomeric BDH.
D000818:Animals; D001051:Apoenzymes / Q000378:metabolism; D002417:Cattle; D003432:Cross-Linking Reagents; D003720:Densitometry; D004789:Enzyme Activation; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000502:physiology*
D001051:Apoenzymes; D003432:Cross-Linking Reagents; D010713:Phosphatidylcholines; D010743:Phospholipids; D006884:Hydroxybutyrate Dehydrogenase