Enhancement of phagocytosis by a calmodulin antagonist (W-7) in mice.
W-7 [N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide], a well-known inhibitor of the calmodulin-dependent processes, strongly enhanced phagocytosis and ingestion of polystyrene latex beads into the peritoneal macrophages of BALB/c mice after the i.p. injection, whereas W-5 [N-(6-aminohexyl)-naphthalene sulfonamide], a less effective calmodulin antagonist, was much weaker than W-7. Binding of the third component of complement (C3) cleavage to the C3 receptor on macrophages after the i.p. injection of W-7 was enhanced as shown by the fluorescent antibody technique. These data suggest that calmodulin plays an important role in the phagocytosis of macrophages through the complement system.
D000818:Animals; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D005260:Female; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects*; D013449:Sulfonamides / Q000494:pharmacology*
D002147:Calmodulin; D013449:Sulfonamides; C017967:W 7; C034700:N-(6-aminohexyl)-1-naphthalenesulfonamide