Measurements of serum glucose using the luciferin/luciferase system and a liquid scintillation spectrometer.
A single-step assay for serum glucose measurements is described. The assay is based on the phosphorylation of D-glucose by glucokinase and the measurement of ATP consumption by firefly luciferase. The luminescence is recorded in an ordinary liquid scintillation spectrometer. The use of stable reagents and a stable final signal (light emission) makes it possible to analyze a large number of samples in each assay run. The assay is of particular value when repeated serum glucose determinations are performed on samples from small laboratory animals.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D049454:Firefly Luciferin; D005941:Glucokinase; D005947:Glucose; D008156:Luciferases; D008163:Luminescent Measurements; D051379:Mice; D008820:Mice, Obese; D010766:Phosphorylation; D013057:Spectrum Analysis / Q000295:instrumentation
D001786:Blood Glucose; D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases; D005941:Glucokinase; D005947:Glucose