[An experimental study on acute rhabdomyolysis in vitro--the influence of a local anesthetic on whole muscle and single muscle fiber function].
I examined the effect of Marcain on the intact and skinned muscle fibers. The whole extensor digitorum longus muscle of rats was mounted in a small bath containing Krebs-Ringer solution. Marcain, when added to the incubation medium, produced a large contracture and markedly increased release of creatine kinase. But the contracture and the release of CK were suppressed with the omission of Ca ion. On pathological examinations of muscle incubated in Marcain solution, muscle fibers were hypercontracted. On the electron microscopy, the sarcomere was shortened and Z-line was partly obscure. The plasmalemma was focally disrupted. In the study on the skinned fiber, Marcain mainly interfered the function of the sarcoplasmic reticulum. Activity of the Ca uptake was inhibited. Ca-induced Ca release and leakage of Ca were facilitated. It was concluded from the present study that regulation of intracellular Ca ion was disturbed by Marcain, causing acute rhabdomyolysis.
D000208:Acute Disease; D000779:Anesthetics, Local / Q000009:adverse effects*; D000818:Animals; D002045:Bupivacaine / Q000009:adverse effects*; D002118:Calcium / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D012206:Rhabdomyolysis / Q000139:chemically induced* / Q000473:pathology; D012519:Sarcoplasmic Reticulum / Q000378:metabolism
D000779:Anesthetics, Local; D003402:Creatine Kinase; D002118:Calcium; D002045:Bupivacaine