title: In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.
abstract: An in vitro assay for curative antibody present in plasma of mice cured of Trypanosoma musculi is described. The assay involves the addition of plasma to a sample of infected blood, followed by hourly monitoring of the parasite count therein. Immune plasma effected a significant reduction in parasite number, whereas normal mouse plasma did not. Heat treatment of immune plasma for 30 min at 56 degrees C abolished its ability to effect any reduction. The assay is simple, rapid, and economical and correlates well with results of all in vivo studies performed to date.
mesh_terms: D000818:Animals; D003165:Complement System Proteins / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004546:Elapid Venoms / Q000494:pharmacology; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014352:Trypanosomiasis / Q000097:blood / Q000276:immunology*
chemical_list: D004546:Elapid Venoms; D007106:Immune Sera; D007136:Immunoglobulins; C016310:cobra venom factor; D003165:Complement System Proteins