Mannose receptor dependent uptake of a ricin A chain--antibody conjugate by rat liver non-parenchymal cells.
Mannose receptor mediated uptake by the reticuloendothelial system has been suggested as an explanation for the rapid removal of ricin A chain antibody conjugates from the circulation after their administration. We have measured, in the rat, hepatic uptake of a ricin A chain antibody conjugate in vivo and its susceptibility to inhibition by a mannosylated protein and have measured uptake of the conjugate in vitro by rat parenchymal and non-parenchymal liver cells. The results indicate that rapid hepatic uptake of conjugate does occur in vivo; cultured non-parenchymal cells accumulate the conjugate to a much greater degree than cultured parenchymal cells and that mannose receptors appear to be involved in the process.
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D004734:Energy Metabolism; D066298:In Vitro Techniques; D037181:Lectins, C-Type; D008099:Liver / Q000378:metabolism*; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000378:metabolism*; D012276:Ricin / Q000378:metabolism*; D014018:Tissue Distribution
D000906:Antibodies; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D012276:Ricin