[Synthesis and functional activity of initiation regions of mRNA translation. II. RNA-ligase synthesis of hepta- and decaribonucleotides--components of 20-base polyribonucleotide templates].
Preparative RNA-ligase synthesis of decaribonucleotides, the 5'-and 3'-constituent parts to be used for the synthesis of 20-base polyribonucleotides] simulating minimal translation initiation regions for phage RNA was carried out. The decamers were obtained via appropriate heptamers also by RNA-ligase catalyzed synthesis. Apart from decamers used to prepare the functionally active 20-base polyribonucleotide, the minimal translation initiation region of the replicase gene (R) in MS2 and fr phage--sequence R(-17----3) and two its variants, decanucleotides for other template modification were also synthesized. Three 5'-terminal decamers were isolated and identified including the natural decamer ApApApCpApUpGpApGpG (-17----(-)8) and those with G(-9)----A(-9) and U(-12)----C(-12) nucleotide substitutions, as well as three 3'-terminal products differing from the natural region ApUpUpCpCpCpApUpG (-7----3) in MS2 RNA by U(-6)----A(-6), U(-6)U(-5)----A(-6)A(-5) and CCC----UUU (-3----(-)1) substitutions.
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D009843:Oligoribonucleotides / Q000096:biosynthesis*; D010442:Peptide Chain Initiation, Translational; D011118:Polynucleotide Ligases; D014176:Protein Biosynthesis; D011130:RNA Ligase (ATP); D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis; D013698:Templates, Genetic
D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)
Sintez i funktsional'naia aktivnost' uchastkov initsiatsii transliatsii mRNR. II. RNK-ligaznyĭ sintez gepta- i dekaribonukleotidov--komponentov 20-chlennykh poliribonukleotidnykh matrits.