A procedure for the preparation of [32P]phosphatidic acid.
The phosphorylation procedure of F. Cramer, W. Rittersdorf, and W. Bohm [(1961) Chem. Ber. 654, 180] using bis(triethylammonium) phosphate and trichloroacetonitrile was shown to be effective in the synthesis of [32P]phosphatidic acid. From diacylglyceride and 0.5 mCi H(3)32PO4, 25-50 microCi of labeled material (sp act = 1 mCi/mumol) can be prepared in 2 h. The product was shown to be radiochemically pure by both TLC and HPLC. L- and DL-[32P]dipalmitoyl phosphatidic acid prepared using this procedure were shown to be hydrolyzed by rat liver microsomes at approximately the same rates.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006868:Hydrolysis; D066298:In Vitro Techniques; D007553:Isotope Labeling / Q000379:methods*; D008862:Microsomes, Liver / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D051381:Rats; D013237:Stereoisomerism
D010712:Phosphatidic Acids; D010761:Phosphorus Radioisotopes; C031599:dipalmitoylphosphatidic acid