Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.
D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004351:Drug Resistance; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005822:Genetic Vectors; D011691:Puromycin / Q000494:pharmacology; D013302:Streptomyces / Q000235:genetics*; D014162:Transfection
D011691:Puromycin; D000123:Acetyltransferases; C045336:puromycin N-acetyltransferase