title: Anion-exchange high-performance liquid chromatography of membrane proteins from liver and Morris hepatomas.
abstract: The separation of proteins from plasma membranes of liver and two Morris hepatomas with different grades of differentiation (Morris hepatomas 7777 and 9121) is described. Size-exclusion high-performance liquid chromatography under non-denaturing conditions provides only poor separation and, with detergent-soluble fractions, unsatisfactory protein recovery. Much better results are obtained by ion-exchange chromatography. The amount of a 175 K protein in the membranes decreased in parallel with the increase in tumour malignancy. This protein appears during ion-exchange chromatography in a broad concentration range, between 0.1 and 0.3 M sodium chloride. Possible explanations for this phenomenon are discussed.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins