title: The two capsid proteins of maize rayado fino virus contain common peptide sequences.
abstract: Virions of maize rayado fino virus (MRFV) were purified and two major capsid proteins (ca. Mr 29,000 and 22,000) were resolved by SDS-PAGE. When the two major capsid proteins were isolated from gels and compared by one-dimensional peptide mapping after digestion with Staphylococcus aureus V-8 protease, indistinguishable peptide maps were obtained, suggesting that these two proteins contain common peptide sequences. Some preparations also showed minor protein components that were intermediate between the Mr 22,000 and Mr 29,000 capsid proteins. One of the minor proteins, ca. Mr 27,000, gave a peptide map indistinguishable from the major capsid proteins. In vitro ageing of partially purified preparations or virion treatment with proteolytic enzymes failed to show conversion of the Mr 29,000 protein to a Mr 22,000. Protease inhibitors added to the buffers used for virion purification did not affect the apparent 1:3 ratio of 29,000 to 22,000 proteins in the purified preparations.
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D010455:Peptides / Q000032:analysis; D010942:Plant Viruses / Q000032:analysis*; D014771:Virion / Q000032:analysis; D003313:Zea mays / Q000382:microbiology
chemical_list: D010455:Peptides