In situ hybridization of an acetylaminofluorene-modified probe recognized by Z-DNA antibodies in vitro.
An in situ hybridization procedure, based on the chemical modification of DNA by acetylaminofluorene (AAF), followed by a specific immunoreaction was used to localize a Z-DNA sequence isolated from the satellite DNA of Cebus appella. The AAF probe is localized on the R-band-positive heterochromatic segments of Cebus chromosomes, which strongly react with Z-DNA antibodies. The use of a nonradioactive single-stranded labeled probe confirms the reliability and the rapidity of immunochemical methods for the detection of DNA sequences on chromosomes.
D015073:2-Acetylaminofluorene; D000818:Animals; D000906:Antibodies / Q000032:analysis; D001483:Base Sequence; D002428:Cebus; D004247:DNA / Q000032:analysis* / Q000276:immunology; D004276:DNA, Satellite / Q000032:analysis* / Q000276:immunology; D007621:Karyotyping; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization
D000906:Antibodies; D004276:DNA, Satellite; D004247:DNA; D015073:2-Acetylaminofluorene