title: Neonatal diethylstilbestrol treatment: response of prolactin to dopamine or estradiol in adult mice.
abstract: The effects of neonatal diethylstilbestrol (DES) treatment on pituitary PRL response to dopamine (DA) inhibition or estrogen stimulation were studied in C3H/MTV+ mice at 10 weeks, 5 months and 8 months of age using synthesis and release of [3H]PRL by the pituitary as endpoints. In the DA study, anterior pituitaries from ovariectomized or intact, neonatally hormone-treated mice were incubated for 6 h in the presence or absence of DA. In the estrogen study, serum and anterior pituitaries were obtained from ovariectomized neonatally hormone-treated mice injected with oil or 17 beta-estradiol for 7 days before death. Neonatal treatment of mice with 2.5 micrograms/day DES altered basal [3H]PRL synthesis and release as well as circulating PRL concentrations. The results of analysis of variance indicated a significant interaction between the effects of neonatal hormone treatment and DA treatment on [3H]PRL release. Neonatal DES treatment did not alter the response of pituitary PRL to estrogen treatment at the ages examined.
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004298:Dopamine / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010052:Ovariectomy; D010902:Pituitary Gland / Q000187:drug effects; D011388:Prolactin / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism
chemical_list: D014316:Tritium; D004958:Estradiol; D004054:Diethylstilbestrol; D011388:Prolactin; D004298:Dopamine