Subcellular distribution of cytochrome P-450 in the brain.
The subcellular distribution of the monooxygenase complexes in the brain was studied by using subcellular fractionation and characterization of these fractions by marker enzymes. Cytochrome P-450 was found to be mainly localized in both synaptic and non-synaptic mitochondria; only a small quantity of enzyme was also found in the microsomal fraction. Peeling off the outer membrane of mitochondria showed that the protein was retained in the inner membrane fraction. A comparative study among some other species confirmed the mitochondrial prevalence of cerebral cytochrome P-450. A partial purification of the rat brain mitochondrial P-450 was obtained.
D000818:Animals; D001923:Brain Chemistry; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000032:analysis; D008297:Male; D008861:Microsomes / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000032:analysis; D013569:Synapses / Q000032:analysis
D003577:Cytochrome P-450 Enzyme System