Demonstration of glucuronic acid on brain glycoproteins which react with HNK-1 antibody.
Ependymins, a family of extracellular glycoproteins of goldfish and mammalian brain, were shown to contain N-linked complex glycan chains. These glycoproteins reacted with a monoclonal antibody, HNK-1 which recognizes a membrane antigen on a subset of human lymphocytes, myelin-associated glycoprotein glycoprotein epitope reacting with HNK-1 antibody was previously shown to include a terminal 3-sulfoglucuronosyl residue present in certain glycolipids of the nervous tissue (Chou et al., Biochem. Biophys. Res. Commun. 1985, 128, 383-388). In this report, the presence of glucuronic acid in ependymins was demonstrated by gas-liquid chromatography and mass spectrometry. We suggest that a 3-sulfoglucuronosyl residue may be the common epitope on HNK-1-reactive glycoproteins.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001923:Brain Chemistry; D002241:Carbohydrates / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006054:Goldfish; D009419:Nerve Tissue Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002241:Carbohydrates; D005965:Glucuronates; D006023:Glycoproteins; D009419:Nerve Tissue Proteins; C031632:ependymins