The orange-yellow pigment of heartwater-infected angora goats.
Heartwater is a highly fatal disease of ruminants in South Africa, caused by the parasite Cowdria ruminantium. A consistent pigment, associated with serum albumin, could be readily extracted from heartwater exudates and serum samples using n-butanol. The pigment gave a characteristic absorption spectrum between 400 and 500 nm which was absent in control serum samples. RP-HPLC of the isolated pigment revealed two components with absorption maxima at 440 and 460 nm and mol. wts of 558 and 1239. The pigment closely resembled bilirubin and/or its conjugates when compared by HPLC.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006357:Heartwater Disease / Q000378:metabolism*; D010860:Pigments, Biological / Q000097:blood / Q000302:isolation & purification*
D000596:Amino Acids; D010860:Pigments, Biological