The effect of a "bisecting" N-acetylglucosaminyl group on the binding of biantennary, complex oligosaccharides to concanavalin A, Phaseolus vulgaris erythroagglutinin (E-PHA), and Ricinus communis agglutinin (RCA-120) immobilized on agarose.
The effect of a "bisecting" 2-acetamido-2-deoxy-beta-D-glucopyranosyl group, linked (1----4) to the beta-D-mannopyranosyl group of asparagine-linked complex and hybrid oligosaccharides, on the binding of [14C]acetylated glycopeptides to columns of immobilized concanavalin A (Con A), Phaseolus vulgaris erythroagglutinin (E-PHA), and Ricinus communis agglutinin-120 (RCA-120) was investigated. The presence of this "bisecting" GlcNAc group caused significant inhibition of the binding to ConA-agarose of biantennary complex glycopeptides in which the two branches are terminated at their nonreducing ends by two GlcNAc groups, or by a Gal and a GlcNAc group, or by two Gal groups, or by a Man and a GlcNAc group. Binding of biantennary, complex glycopeptides to E-PHA-agarose required a "bisecting" GlcNAc group, a Gal group at the nonreducing terminus of the alpha-D-Man-p-(1----6) branch, and a terminal or internal GlcNAc residue linked beta-(1----2) to the alpha-D-Manp-(1----3) branch. Binding to RCA-120-agarose occurred only when at least one nonreducing terminal Gal group was present, and increased as the proportion of terminal Gal groups increased; the presence of a "bisecting" GlcNAc group caused either enhancement or inhibition of these binding patterns. It is concluded that a "bisecting" GlcNAc group affects the binding of glycopeptides to all three lectin columns.
D000089:Acetic Anhydrides; D000107:Acetylation; D000117:Acetylglucosamine; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D002367:Ricinus communis; D003208:Concanavalin A; D005944:Glucosamine / Q000031:analogs & derivatives; D006020:Glycopeptides / Q000302:isolation & purification; D037102:Lectins; D009844:Oligosaccharides / Q000302:isolation & purification; D010835:Phytohemagglutinins; D037121:Plant Lectins; D010947:Plants, Toxic; D012276:Ricin; D012685:Sepharose
D000089:Acetic Anhydrides; D002250:Carbon Radioisotopes; D006020:Glycopeptides; D037102:Lectins; D009844:Oligosaccharides; D010835:Phytohemagglutinins; D037121:Plant Lectins; D003208:Concanavalin A; C031800:acetic anhydride; D012276:Ricin; D012685:Sepharose; D005944:Glucosamine; D000117:Acetylglucosamine