Bioavailability of 1-nitropyrene from model coal fly ash and its uptake by alveolar macrophages.
Alveolar macrophage cultures exposed to coal fly ash vapor-coated with 1-nitropyrene were used as a model system to study the bioavailability and the uptake of a nitroaromatic hydrocarbon from coal combustion emissions. Initially, 1-nitropyrene-coated fly ash and uncoated fly ash were examined for cytotoxicity using rabbit alveolar macrophages and for mutagenicity in the Salmonella typhimurium plate incorporation assay. The results were compared to determine the effects of vapor deposition. The distribution and recovery of 1-nitropyrene from macrophage cultures treated with coated fly ash were determined by using a reverse-phase high-performance liquid chromatography-fluorescence method. 1-Nitropyrene alone was not very toxic, nor did vapor deposition of 1-nitropyrene onto coal fly ash significantly affect the toxicity of the fly ash. Most toxicity resulted from the original, uncoated fly ash particles. 1-Nitropyrene after being coated onto the particles was bioavailable in agar and aqueous culture medium. The coated fly ash showed mutagenic activity when the particles were tested directly; the uncoated fly ash did not show mutagenic activity. 1-Nitropyrene recovery from alveolar macrophage cultures exposed to the coated fly ash diminished as cell number increased. The rate of 1-nitropyrene loss was 2.7 ng/10(6) macrophages for medium and 4.1 ng/10(6) macrophages for the whole culture. The mutagenic activity recovered from these macrophage cultures also decreased with increasing cell number.
D000818:Animals; D001682:Biological Availability; D002244:Carbon / Q000633:toxicity*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D060729:Coal Ash; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009152:Mutagenicity Tests; D052638:Particulate Matter; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism*; D011817:Rabbits
D060729:Coal Ash; D052638:Particulate Matter; D011721:Pyrenes; D002244:Carbon; C032668:1-nitropyrene