title: Quantitative analysis of polyenoic phospholipid molecular species by high performance liquid chromatography.
abstract: The quantitative analysis of phospholipid molecular species containing polyenoic fatty acids is described. Dinitrobenzoyl derivatives of diacylglycerols prepared from phospholipids were separated into individual molecular species by reversed-phase high performance liquid chromatography (HPLC) using a combination of two solvent systems and were quantified at 254 nm. Thirty-six molecular species were resolved from the phosphatidylcholines of rat hearts, human platelets and Chinese hamster V79-R cells. The derivatives of alkenylacyl molecular species from platelet phosphatidylethanolamine were resolved concomitantly with diacyl molecular species.
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D010743:Phospholipids / Q000032:analysis*; D011090:Polyenes / Q000032:analysis*; D013329:Structure-Activity Relationship
chemical_list: D007202:Indicators and Reagents; D010743:Phospholipids; D011090:Polyenes