Protein phosphorylation in intact cultured sycamore (Acer pseudoplatanus) cells and its response to fusicoccin.
Fusicoccin (FC), a natural diterpene glucoside able to stimulate electrogenic H+ extrusion in higher plants, has been shown to stimulate the phosphorylation of a polypeptide of molecular mass approx. 33 kDa in intact cultured cells of sycamore (Acer pseudoplatanus). The effect is specific, rapid and insensitive to cycloheximide. The presence of the 33 kDa polypeptide and the stimulation by FC have been observed in SDS-containing cell homogenates and in the microsomal and soluble fractions after cell fractionation.
D003513:Cycloheximide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006027:Glycosides / Q000494:pharmacology*; D010766:Phosphorylation; D059828:Plant Cells; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000187:drug effects; D014197:Trees
D006027:Glycosides; D010940:Plant Proteins; C007808:fusicoccin; D003513:Cycloheximide