Isolation and characterization of spicule proteins from Strongylocentrotus purpuratus.
A simple method is described for the isolation of spicules from pluteus embryos of the sea urchin, Strongylocentrotus purpuratus. Radio-iodination of the demineralized matrix reveals six bands on SDS protein gels. Treatment with N-glycanase leads us to believe that some of these proteins are N-linked glycoproteins.
D000818:Animals; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D006026:Glycoside Hydrolases; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D012617:Sea Urchins / Q000032:analysis* / Q000196:embryology
D006023:Glycoproteins; D011506:Proteins; D006026:Glycoside Hydrolases; C020668:glycanase