Li+ uptake into Xenopus and Cynops oocytes injected with exogenous mRNA, observed by flame emission spectroscopy.
Li+ uptake into Xenopus oocytes was measured by flame emission spectroscopy. Li+ uptake into the oocytes increased proportionally with incubation time and was dependent on either pH or temperature. Maximum uptake of Li+ was observed around pH 7. Li+ uptake into Xenopus oocytes increased by a factor of roughly 7 over the range 4-30 degrees C. When mRNA prepared from electroplax of Electrophorus electricus was injected into Xenopus or Cynops oocytes, Li+ uptake into the injected oocytes increased by the addition of carbamylcholine (Carb), an agonist of the acetylcholine receptor (AChR). This increase of Li+ uptake by Carb was inhibited by d-tubocurarine, an antagonist of nicotinic AChR. Thus, a new method was established for detection of the activity of nicotinic AChR synthesized in oocytes injected with exogenous mRNA.
D000818:Animals; D004557:Electric Organ; D004593:Electrophorus; D005260:Female; D006863:Hydrogen-Ion Concentration; D008094:Lithium / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000502:physiology; D012455:Salamandridae; D013696:Temperature; D013997:Time Factors; D014981:Xenopus
D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D008094:Lithium