Determination of halogenated contaminants in human adipose tissue.
A method has been developed for determination of organochlorine contaminants in human adipose tissue. After fat extraction from the tissue with acetone-hexane (15 + 85, v/v), organochlorines were fractionated from fat by gel permeation chromatography with methylene chloride-cyclohexane (1 + 1, v/v) as solvent. After Florisil column cleanup, the GPC extract was analyzed by capillary column gas chromatography using 2 columns of different polarity. Compound identity was confirmed by gas chromatography-mass spectrometry using selected ion monitoring. Recoveries for fortification levels of 10-500 ng/g were greater than 80% except for trichlorobenzene and hexachlorobutadiene (ca 60%).
D000273:Adipose Tissue / Q000032:analysis*; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D004785:Environmental Pollutants / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006846:Hydrocarbons, Halogenated / Q000032:analysis*
D004785:Environmental Pollutants; D006846:Hydrocarbons, Halogenated