Nuclear and mitochondrial DNA synthesis and energy metabolism in primary rat glial cell cultures.
DNA synthesis in nuclei and mitochondria purified from serum-supplemented rat glial cell cultures at different days after plating was studied. Furthermore in mitochondria, some enzymatic activities related to energy transduction (citrate synthase, malate dehydrogenase, total NADH-cytochrome c reductase, cytochrome oxidase and glutamate dehydrogenase) were measured. For DNA labeling [methyl-3H]thymidine was added to the culture medium at different days after plating. During the culture times studied the specific activity of total, nuclear, and mitochondrial DNA decreased from 8 days in vitro (DIV) to 21 DIV and increased at 30 DIV. The specific activity of nuclear DNA was always higher than that of mitochondrial DNA. The specific activity of the above mentioned mitochondrial enzymes increased from 8 DIV up to 21 DIV and decreased at 30 DIV, suggesting a relationship between the energy metabolism and the differentiation of glial cells in culture.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000378:metabolism*; D004734:Energy Metabolism; D008928:Mitochondria / Q000201:enzymology; D009457:Neuroglia / Q000378:metabolism*; D051381:Rats; D013936:Thymidine; D013997:Time Factors
D004272:DNA, Mitochondrial; D013936:Thymidine