An immunological characterization of five common antigens of chromaffin granules and of large dense-cored vesicles of sympathetic nerve.
Bovine chromaffin granules and large dense-cored vesicles of bovine splenic nerve were compared by one- and two-dimensional immunoblotting. Both types of vesicles contain chromogranin A, B and C. However, the proteolytic processing of these chromogranins within these vesicles is apparently different. Chromogranin B in chromaffin granules is processed by more than 80% whereas in nerve vesicles only 15% has been broken down to smaller proteins. In addition both types of vesicles contain dopamine beta-hydroxylase and cytochrome b-561.
D000818:Animals; D000941:Antigens / Q000032:analysis; D002417:Cattle; D002837:Chromaffin Granules / Q000032:analysis*; D002838:Chromaffin System / Q000032:analysis*; D002864:Chromogranins / Q000032:analysis* / Q000276:immunology; D003573:Cytochrome b Group / Q000032:analysis* / Q000276:immunology; D004299:Dopamine beta-Hydroxylase / Q000032:analysis* / Q000276:immunology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D013564:Sympathetic Nervous System / Q000032:analysis* / Q000276:immunology
D000941:Antigens; D002864:Chromogranins; D003573:Cytochrome b Group; D009419:Nerve Tissue Proteins; C023646:cytochrome b561; D004299:Dopamine beta-Hydroxylase