[Secretion of alpha-L-fucosidase by human embryonal skin fibroblasts].
The amount of alpha-L-fucosidase secreted by normal human fibroblasts was higher in the medium containing 10% bovine serum than in the medium containing 0.1% bovine serum. Glycosidase secretion was twice higher at the advanced than at the initial stage of subcultivation. Extracellular activity of alpha-L-fucosidase from 3 different fibroblast strains differed insignificantly in the medium containing 0.1% bovine serum, while intracellular activity of the enzyme in these strains was altogether different. The results suggest that the lysosomal glycosidase secretion is determined by the level of cellular endocytosis.
D003470:Culture Media; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D012867:Skin / Q000196:embryology* / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000378:metabolism*
D003470:Culture Media; D005644:alpha-L-Fucosidase
Sekretsiia al'fa-L-fukozidazy émbrional'nymi kozhnymi fibroblastami cheloveka.