[Automated microanalysis of plasma ceruloplasmin for the measurement of oxidase activity in cattle and sheep].
An automated method for the determination of plasma ceruloplasmin in bovine and ovine species is described. The analytic principle of Holmberg and Laurell (1951), based on protein oxidase activity, has been applied to a discrete computerized analyzer (Isamat, Isa Biologie, 94230 Cachan, France). Using this method a single operator can determine the oxidase activity of 500 plasma ceruloplasmin samples per day. The method is accurate (2.6% precision for mean values), linear over a range of 7-240 mcg/100 ml in copper equivalents and low in cost because small volumes of reagents can be used; it also correlates well with results from standard atomic absorption spectrophotometry and single radial immunodiffusion methods (correlation coefficients: 0.944 to 0.968). The conservation of oxidase activity is adequate and plasma ceruloplasmin oxidase activity determination can be used for assessing copper nutritional state and for screening inflammatory processes in bovine and ovine herds.
D000818:Animals; D001322:Autoanalysis; D002417:Cattle; D002570:Ceruloplasmin / Q000032:analysis*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008832:Microchemistry; D010088:Oxidoreductases / Q000097:blood*; D012756:Sheep; D013045:Species Specificity
D010088:Oxidoreductases; D002570:Ceruloplasmin
Micro-dosage automatisé de la céruloplasmine plasmatique par mesure de l'activité oxydasique chez les bovins et les ovins.