[Hydroxylation of steroids in a dienzyme system consisting of cytochrome P-450 and immobilized adrenodoxin].
Experimental systems for the hydroxylation of steroids (11-deoxycorticosterone and cholesterol) with reduced electron transfer chain, in which flavoprotein was omitted, were investigated. Incubation of chemically reduced immobilized adrenodoxin either with cytochrome P-45011 beta or cytochrome P-450scc in the presence of substrate of hydroxylation and oxygen yields the specific reaction products, corticosterone or pregnenolone. The catalytic activity of the experimental dienzyme systems proves the possibility of the steroid hydroxylation mechanism based exclusively on dissociation and reassociation of the electron transporting protein complexes.
D000311:Adrenal Glands / Q000201:enzymology; D000325:Adrenodoxin / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004800:Enzymes, Immobilized; D006900:Hydroxylation; D066298:In Vitro Techniques; D008928:Mitochondria / Q000201:enzymology; D009097:Multienzyme Complexes / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism*
D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D013256:Steroids; D000325:Adrenodoxin; D003577:Cytochrome P-450 Enzyme System
Gidroksilirovanie steroidov v difermentnoĭ sisteme, sostoiashcheĭ iz tsitokhroma P-450 i immobilizirovannogo adrenodoksina.