title: Distinguishing between ovine abortion and ovine arthritis Chlamydia psittaci isolates with specific monoclonal antibodies.
abstract: Monoclonal antibodies to Chlamydia psittaci were prepared by both in vivo and in vitro immunization methods, using an abortion strain of C psittaci as the immunizing antigen. Seven of the 8 monoclonal antibodies produced were genus-specific by the enzyme-linked immunosorbent assay and immunofluorescence test. The genus-specific antibodies were reactive with a protease-resistant, periodate-sensitive antigen of less than 14 kilodaltons. The remaining monoclonal antibody, 10D7, was specific for ovine abortion strains of C psittaci and nonreactive with 2 strains isolated from the joints of lambs with polyarthritis. The type-specific antigen was protease sensitive, but could not be detected in the immunoblot assay.
mesh_terms: D000034:Abortion, Veterinary; D000818:Animals; D000911:Antibodies, Monoclonal; D001170:Arthritis, Infectious / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000276:immunology / Q000302:isolation & purification*; D003937:Diagnosis, Differential; D005260:Female; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D009956:Psittacosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D000911:Antibodies, Monoclonal