Role of rat liver inducible enzymes in in vitro metabolic transformation of 1-nitropyrene.
Liver enzymes (S9) of rats induced with various drugs differently affected the mutagenic response of 1-nitropyrene (1-NP) in vitro. The role of inducible enzymes in 1-NP conversion was evaluated by high-performance liquid chromatography (HPLC) analyses of 1-NP metabolites formed during incubation in vitro of the chemical with each S9 fraction. Methylcholanthrene (MC)-induced S9 preferentially results in 1-NP hydroxylation in position 6 or 8. Metabolic interconversions among 1-NP metabolites could be inferred from the HPLC patterns.
D000818:Animals; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology*; D004790:Enzyme Induction; D006900:Hydroxylation; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D009153:Mutagens / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D009153:Mutagens; D011721:Pyrenes; D003577:Cytochrome P-450 Enzyme System; C032668:1-nitropyrene