Improved large scale purification procedure of natural human fibroblast interferon.
Human fibroblast interferon, produced in tissue culture by a superinduction procedure, was purified 10,000-fold by chromatography to ca 50% purity as judged by SDS-PAGE. The specific activity of the interferon preparation is 2 X 10(8) and the yield is 70%. The interferon preparation is suitable for clinical use.
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004731:Endotoxins / Q000302:isolation & purification; D006801:Humans; D007370:Interferon Type I / Q000302:isolation & purification*; D008970:Molecular Weight; D011749:Pyrogens / Q000032:analysis
D004731:Endotoxins; D007370:Interferon Type I; D011749:Pyrogens