[The 31P-NMR study of the metabolism of phosphate-containing compounds in the rat liver during inhibition of protein synthesis].
Using the 31P-NMR method, the composition of the pool of phosphate-containing metabolites in intact rat liver 72 hours following the blocking of protein biosynthesis by cycloheximide was studied. It was shown that during maximal inhibition, i.e., 2-3 hours after cycloheximide injection, the ATP concentration decreases approximately 5-fold, that of ADP and sugar phosphates--4- and 2-fold, respectively. The intracellular pH in hepatocytes was followed by measuring the chemical shift of the Pi signal. The reconstitution of intracellular pH after 2-3 hours is consistent with changes in the Pi level in hepatocytes. The experimental results were compared with the data of biochemical analysis. NMR seems to be a promising tool in the study of metabolism of various animal organs and tissues under physiological and pathological conditions.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D003864:Depression, Chemical; D005215:Fasting; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D013403:Sugar Phosphates / Q000378:metabolism; D013997:Time Factors
D010710:Phosphates; D013403:Sugar Phosphates; D003513:Cycloheximide
Izuchenie metabolizma fosfatsoderzgashchikh soedineniĭ v pecheni krysy metodom 31P-IaMR v usloviiakh podavleniia sinteza belka.