Development of an homologous radioimmunoassay for the synthetic amino terminal (1-34) fragment of human parathyroid hormone using egg yolk-obtained antibodies.
An homologous radioimmunoassay for the synthetic 1-34 amino terminal fragment of human parathyroid hormone (hPTH) was developed using antibodies obtained from yolk of eggs layed by an immunized chicken. The 125I labelled 1-34 hPTH peptide was purified by cation-exchange chromatography which provided a highly stable preparation. The specificity of the assay showed a cross-reactivity of 50% with the 1-34 hPTH (code 81/574) preparation and 27% with the 1-84 hPTH (code 79/500) preparation, when compared with the 1-34 hPTH from Bachem that was used for immunization and labelling. The minimal detectable dose of the assay was 10 pmol/l; in 69 healthy controls the values obtained ranged from less than 10 to 28 pmol/l and in 14 patients with surgically proven primary hyperparathyroidism from 10 to 519 pmol/l.
D000818:Animals; D000906:Antibodies; D002118:Calcium / Q000097:blood; D002645:Chickens; D004530:Egg Yolk; D005260:Female; D006801:Humans; D006961:Hyperparathyroidism / Q000097:blood; D010281:Parathyroid Hormone / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D019379:Teriparatide
D000906:Antibodies; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide; D002118:Calcium