Determination of intracellular potassium ion concentration in isolated rat ventricular myocytes.
The sarcoplasmic potassium concentration of a suspension of rat ventricular myocytes, prepared by collagenase-induced disruption of the myocardial mass, was determined by a null-point technique. Addition of digitonin resulted in a release of potassium from the cells which was interpreted as a flux from the sarcoplasm. The intracellular potassium concentration was estimated to be 113 +/- 6mM.
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D006352:Heart Ventricles / Q000166:cytology; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D012508:Sarcolemma / Q000502:physiology
D011188:Potassium