title: A simple borohydride/GC method for measuring sparteine metabolites in man.
abstract: A simple borohydride/GC method was developed for phenotyping sparteine oxidation in man. The major metabolites of sparteine found in human urine, 2- and 5-dehydrosparteine, were converted quantitatively back to sparteine by sodium borohydride reduction. The amount of sparteine metabolites can be estimated from the difference of sparteine concentrations between the borohydride-treated and untreated urine samples. The coefficient of variation of this assay was estimated from repeated analyses to be +/- 3% within a day (intra-assay) and +/- 8% between days (inter-assay).
mesh_terms: D001894:Borohydrides; D002849:Chromatography, Gas / Q000379:methods*; D014644:Genetic Variation; D006801:Humans; D010084:Oxidation-Reduction; D010641:Phenotype; D013034:Sparteine / Q000378:metabolism*; D014556:Urine / Q000032:analysis
chemical_list: D001894:Borohydrides; D013034:Sparteine; C025364:sodium borohydride