A high melting cis-[Pt(NH3)2[d(GpG)]]adduct of a decanucleotide duplex.
The [cis-Pt(NH3)2(d(GCCGGATCGC)-N7(4), N7(5))]-d(GCGATCCGGC) duplex has been prepared with Tm = 49 degrees C (vs 58 degrees C for the unplatinated form). NMR of the ten observable imino protons supports a kinked structure with intact base pairing of the duplex on the 3'-side of the d(GpG).cis-Pt chelate (relative to the platinated strand) The modification of the B-DNA type CD spectrum, due to the platinum chelate, is comparable to that observed for the platination (at a 0.05 Pt:base ratio) of the Micrococcus Lysodeikticus DNA (72% GC).
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides; D013054:Spectrophotometry, Atomic
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D002945:Cisplatin