Simplified non-enzymatic technique for isolation of epithelium and muscle from the dog prostate gland.
Epithelium and muscle from the normal dog prostate were isolated with a rapid, simple, non-surgical separation (NSS) technique, involving mechanical agitation of organ cubes in a high concentration (30 microM) of EDTA for tissue dispersion, and gentle pressure filtration for recovery of epithelium and muscle. Derivation of this NSS procedure utilized the guinea pig seminal vesicle, since it can be surgically separated (SS) into pure epithelium and muscle to serve as controls for the biochemical viability of NSS prepared tissues. The NSS procedure adopted for use yielded not only pure (greater than 95%) epithelium and muscle from intact seminal vesicle, but also activities of 5 alpha-reductase and concentrations of the cytosol estrophile in each tissue, which were not significantly different from the SS counterparts. In the dog prostate gland, the concentrations of 5 alpha-reductase and the cytosol estrophile in the NSS epithelium were 1.58 and 0.43 of the NSS muscle, respectively.
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism; D000818:Animals; D004848:Epithelium / Q000033:anatomy & histology / Q000201:enzymology; D004958:Estradiol / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D009130:Muscle, Smooth / Q000033:anatomy & histology* / Q000201:enzymology; D011467:Prostate / Q000033:anatomy & histology* / Q000201:enzymology; D012669:Seminal Vesicles / Q000033:anatomy & histology / Q000201:enzymology
D004958:Estradiol; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase