title: Ependyma formation in adult rat spinal cord after transplantation of fetal cerebral cortex homografts.
abstract: The ependymal cells in rat host spinal cord were studied after transplantation of fetal cerebral cortex homografts. Adult Sprague-Dawley male rats had pieces of E14 fetal rat cortex pressure-injected into the spinal cord at T6. The tissue was prepared for light and electron microscopy and studied for over 2 months postimplantation. At 7 and 14 days postimplantation (DPI), there were dividing ventricular-ependymal cells lining cysts in the host spinal cord. After 21 days, cell division was no longer observed in these cells, and only mature ependymal cells lined these cysts. Mature ependymal cells were either: columnar, pseudocolumnar, cuboidal, squamous, or pseudomorphic; had cilia and villi on only one surface of the cell and shared tight junctions when contiguous. These data show that ependymal cells were mature cellular components of adult spinal cord after transplantation of fetal CNS grafts. In addition, ventricular-ependymal cells continued to divide within the parenchyma of the graft, indicating continued growth of transplanted fetal cerebral cortex in host spinal cord.
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology / Q000637:transplantation*; D004805:Ependyma / Q000502:physiology* / Q000648:ultrastructure; D005333:Fetus; D006085:Graft Survival; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*