title: Q fever vaccination of sheep: challenge of immunity in ewes.
abstract: Adult ewes (17 months of age) were vaccinated against Coxiella burnetii, using a formalin-inactivated whole cell (WC) phase I Henzerling strain vaccine or a chloroform methanol residue (CMR) vaccine. Nineteen pregnant ewes were placed in 3 categories [(i) unvaccinated, (ii) WC vaccine, and (iii) CMR vaccine] and were challenge exposed at approximately the 100th day of gestation with 210,000 plaque-forming units of C burnetii inoculated subcutaneously. Shedding of rickettsiae was measurably reduced, but was not prevented in vaccinated groups, as shown by inoculating ewes' placental tissues, amniotic fluid, and colostrum into mice, as well as by histopathologic lesions of placental tissues. The rickettsiae were shed in the placenta, amniotic fluid, or colostrum in 6 nonvaccinated ewes. In comparison, rickettsiae were detected in placental inoculations from 2 of 6 ewes in the WC vaccine group and 1 of 6 in the CMR group. In contrast to those in the vaccinated ewes, placentitis, high concentrations of rickettsiae in microscopic preparations, and weak lambs were typical for the nonvaccinated ewes.
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D003381:Coxiella / Q000276:immunology*; D005260:Female; D011247:Pregnancy; D011778:Q Fever / Q000517:prevention & control* / Q000662:veterinary; D012290:Rickettsial Vaccines / Q000008:administration & dosage*; D012756:Sheep / Q000276:immunology*; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D012290:Rickettsial Vaccines; D014612:Vaccines