title: Cytochemical calcium distribution in secretory ameloblasts of the rat in relation to enamel mineralization.
abstract: Calcium distribution in secretory ameloblasts was studied in rat incisor enamel in which mineralization was temporarily disturbed by injection of either fluoride or cobalt. Pyroantimonate precipitates of calcium were analysed morphometrically in regions of the cell membranes, mitochondria and secretory granules. The disturbances in mineralization were characterized by accumulations of unmineralized enamel matrix at the secretory regions of Tomes' process within 1 h after injection. Fluoride-induced disturbances in mineralization were not accompanied by marked changes in calcium concentration and distribution. It may be that fluoride causes alterations in the synthesis and secretion of the organic matrix which affects its ability to mineralize. Secretory ameloblasts treated with cobalt showed a broad basis for interference with calcium, in particular that which is associated with cell membranes and secretory granules. Secretory ameloblasts may be actively controlling the availability of calcium to enamel by mechanisms involving the cell membrane as well as the secretory granules.
mesh_terms: D000565:Ameloblasts / Q000378:metabolism*; D000818:Animals; D000965:Antimony / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology; D003743:Dental Enamel / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005459:Fluorides / Q000494:pharmacology; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D008903:Minerals / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D008903:Minerals; C009133:pyroantimonate; D003035:Cobalt; D000965:Antimony; D005459:Fluorides; D002118:Calcium