title: Trypanosoma brucei: cis-aconitate and temperature reduction as triggers of synchronous transformation of bloodstream to procyclic trypomastigotes in vitro.
abstract: Synchronous transformation of the monomorphic Trypanosoma brucei 427 variant clone MITat 1.4 (117) from bloodstream to procyclic trypomastigotes was studied in modified minimum essential medium plus 15% inactivated horse serum. Repression of variant surface glycoprotein synthesis, subsequent morphological transformation, and growth of procyclic cells was triggered by the simultaneous action of two signals: a reduction in temperature from 37 to 27 C and the addition of cis-aconitate. Repression of variant surface glycoprotein synthesis initiated by these two signals is reversible during the first hours, but becomes irreversible after about 1 day. Thereafter, cells are committed to differentiation at 27 C.
mesh_terms: D000156:Aconitic Acid / Q000494:pharmacology*; D000818:Animals; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D003470:Culture Media; D006023:Glycoproteins / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis; D013696:Temperature; D014233:Tricarboxylic Acids / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D014643:Variant Surface Glycoproteins, Trypanosoma
chemical_list: D002951:Citrates; D003470:Culture Media; D006023:Glycoproteins; D008565:Membrane Proteins; D014233:Tricarboxylic Acids; D014643:Variant Surface Glycoproteins, Trypanosoma; D019343:Citric Acid; D000156:Aconitic Acid