Spectral shift of fluorescein and carboxyfluorescein in the anterior chamber of the rabbit eye following systemic administration.
Systemically administered fluorescein is converted to fluorescein glucuronide. The conjugate is fluorescent and interferes with the measurement of fluorescein in the anterior chamber. Carboxyfluorescein is a hydrophilic derivative of fluorescein. In the rabbit, carboxyfluorescein is not converted as readily to a fluorescent metabolite. Thus, carboxyfluorescein has potential advantages as a quantitative fluorophore for studies of aqueous humor dynamics.
D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism*; D002845:Chromatography; D019793:Fluorescein; D005452:Fluoresceins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005453:Fluorescence; D005470:Fluorometry; D007275:Injections, Intravenous; D010783:Photometry; D011817:Rabbits
D005452:Fluoresceins; C024098:6-carboxyfluorescein; D019793:Fluorescein