Construction of a genomic library from germinating seedlings of mung bean (Vigna radiata). Evidence for the presence of a class of repetitive sequences and selection of beta-tubulin specific recombinant phage.
We have constructed a genomic library of Vigna radiata from its total DNA in the phage charon 4A. Recombinant phages were used to hybridize with a family of mung bean repetitive DNA. At least 30% of recombinant phages showed positive hybridization with a specific type of sequences. These phages were also used to screen for beta-tubulin-specific recombinants and a recombinant phage with beta-tubulin gene was identified.
D001345:Autoradiography; D001435:Bacteriophages / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis*; D007887:Fabaceae / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010946:Plants, Medicinal; D012091:Repetitive Sequences, Nucleic Acid; D014404:Tubulin / Q000235:genetics*
D004274:DNA, Recombinant; D014404:Tubulin