title: Enzyme electrode for the measurement of lactate in undiluted blood.
abstract: An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H2O2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l L-lactate with response times of 1-3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave y = 0.96x + 0.11, r = 0.996, n = 46.
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives; D004058:Diffusion; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D006899:Mixed Function Oxygenases; D012821:Silanes
chemical_list: D007773:Lactates; D012821:Silanes; D019344:Lactic Acid; C005062:acetylcellulose; D002482:Cellulose; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase; C049552:methyltrichlorosilane