[Various properties of immobilized AMP-aminohydrolase].
Properties of immobilized AMP-aminohydrolase from rabbit muscles are studied. The enzyme retains its activity for a year, is stable under manifold treatment with the substrate or under single treatment with 1 M NaCl which contains 50% ethylene glycol or 10% isopropanol and under treatment with 5 M K2 HPO4 (pH 8.5). The established pH-optimum (6.5-7.0) and the temperature optimum (30-40 degrees C) for immobilized AMP-aminohydrolase as well as inhibition of its activity by Co2+, Cd2+, Zn2+ and n-chloromercury benzoate indicate similarity of its properties with those of the purified enzyme.
D000659:AMP Deaminase / Q000032:analysis* / Q000037:antagonists & inhibitors; D000818:Animals; D004800:Enzymes, Immobilized / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D009709:Nucleotide Deaminases / Q000032:analysis*; D011817:Rabbits; D013379:Substrate Specificity; D013997:Time Factors
D004800:Enzymes, Immobilized; D009709:Nucleotide Deaminases; D000659:AMP Deaminase
Nekotorye svoĭstva immobilizovannoĭ AMP-aminogidrolazy.