Current concepts in membrane protein reconstitution.
The reconstitution of integral proteins into artificial lipid vesicles is largely prompted by the complexity of most biological membranes and the inherent difficulty of studying individual components in situ. Ideally, therefore, the reconstituted system should consist of a single protein in a lipid matrix which mimics the native membrane in all but its diversity. While such an approach allows individual components of a complex system to be studied in isolation it should also be sufficiently versatile to permit the generation of increasingly sophisticated multicomponent models. From the considerable number of reconstitution techniques which have been developed I have tried in this review to identify those characteristics of a particular system which maximise both the information it can provide and its versatility.
D003902:Detergents; D005614:Freeze Fracturing; D005615:Freezing; D008563:Membrane Lipids / Q000302:isolation & purification / Q000502:physiology; D008565:Membrane Proteins / Q000302:isolation & purification / Q000502:physiology*; D008566:Membranes / Q000201:enzymology; D008854:Microscopy, Electron
D003902:Detergents; D008563:Membrane Lipids; D008565:Membrane Proteins