Mechanism of nitrogen regulation of protylonolide biosynthesis in Streptomyces fradiae.
A resting cell system was used to study the sites of inhibition by NH4+ of protylonolide biosynthesis by a blocked mutant, strain 261, of Streptomyces fradiae. With 14C-labeled succinate or valine as an exogenous substrate, labeled protylonolide formation by high-NH4+ grown mycelia of strain 261 was lower than by low-NH4+ grown mycelia. When 14C-labeled palmitate or acetate + labeled propionate + butyrate were used as the substrates, protylonolide production by mycelia grown under the two NH4+ conditions was nearly at the same rates. It is suggested that the metabolism of succinate and valine to lower fatty acid precursors is subject to NH4+ regulation, whereas condensation of acid precursors and related steps leading to protylonolide are insensitive to NH4+ concentration.
D002250:Carbon Radioisotopes; D005227:Fatty Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007933:Leucomycins / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D015645:Tylosin / Q000031:analogs & derivatives
D002250:Carbon Radioisotopes; D005227:Fatty Acids; D007933:Leucomycins; D013386:Succinates; C027411:protylonolide; D019802:Succinic Acid; D009584:Nitrogen; D015645:Tylosin