Transplantation of bovine mammary tissue to athymic nude mice: growth subcutaneously and in mammary gland-free fat pads.
Mammary epithelium from five Holstein cows was transplanted as subcutaneous slices and as collagenase dissociated epithelial cells into mammary gland free-fat pads of athymic nude mice. Two weeks posttransplantation, mice were injected daily for 20 d with saline, 17 beta-estradiol plus progesterone, or 17 beta-estradiol plus progesterone plus growth hormone plus prolactin. In a second experiment, mice were treated with saline, cholera toxin, 17 beta-estradiol (subcutaneous pellet of 2 mg 17 beta-estradiol and 38 mg cholesterol), or 17 beta-estradiol plus cholera toxin. In each experiment mammary slices maintained normal morphology. Growth of epithelium within slices ([3H]thymidine autoradiography) was increased 167% by estradiol plus progesterone, 264% by estradiol plus progesterone plus growth hormone plus prolactin, 90% by estradiol, 60% by cholera toxin, and 137% by estradiol plus cholera toxin. Cells injected into mammary gland free fat pads formed hollow, multilayered, spherical "organoids." Organoid area was increased 47% by estradiol plus progesterone, 189% by estradiol plus progesterone plus growth hormone plus prolactin, 72% by estradiol, 86% by cholera toxin and 74% by estradiol plus cholera toxin. Thus, athymic nude mice appear suitable for the ex vivo in vivo study of bovine mammary epithelial growth and differentiation.
D000818:Animals; D002417:Cattle; D002772:Cholera Toxin / Q000494:pharmacology; D004338:Drug Combinations; D004848:Epithelium / Q000637:transplantation; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000254:growth & development / Q000637:transplantation*; D051379:Mice; D008819:Mice, Nude; D011374:Progesterone / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology; D014183:Transplantation, Heterologous
D004338:Drug Combinations; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D013006:Growth Hormone; D002772:Cholera Toxin