Identification of glucuronide metabolites of T-2 toxin and diacetoxyscirpenol in the bile of isolated perfused rat liver.
Isolated rat livers were perfused with either 2 mg T-2 toxin or diacetoxyscirpenol (DAS) in a recirculating perfusion system. To identify glucuronide conjugates, equal amounts of bile samples were incubated with and without (control) a beta-glucuronidase preparation and analyzed by capillary gas liquid chromatography-chemical ionization mass spectrometry. Enzyme treatment of bile obtained from liver perfused with T-2 toxin resulted in the detection of a total of 954 micrograms HT-2 toxin (control 6 micrograms), demonstrating that excretion into the bile was mainly as glucuronide conjugates. Minor metabolites of T-2 toxin in bile were identified as 3'-hydroxy HT-2 toxin (TC-3), 3'-hydroxy-7-hydroxy HT-2 toxin (TC-6), and the glucuronide form of T-2 triol (trace amount). The glucuronide conjugates of monoacetoxyscirpenol (340 micrograms) and scirpenetriol (10 micrograms) were found in bile obtained from liver perfused with DAS, while nonconjugated metabolites were not detected. It is assumed that considerable amounts of T-2 toxin and DAS were metabolized biphasically. In phase I both trichothecenes were deacetylated, in phase II the metabolites were conjugated giving rise to the glucuronic acid adducts.
D000818:Animals; D001646:Bile / Q000032:analysis*; D005965:Glucuronates / Q000032:analysis*; D005966:Glucuronidase / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000378:metabolism*; D013605:T-2 Toxin / Q000378:metabolism*; D014255:Trichothecenes / Q000378:metabolism*
D005965:Glucuronates; D012717:Sesquiterpenes; D014255:Trichothecenes; D005966:Glucuronidase; D013605:T-2 Toxin; C001809:diacetoxyscirpenol