Hydrophobic behavior of sciatin and transferrin on a size-exclusion column.
Sciatin and transferrin are very similar glycoproteins which differ slightly in their carbohydrate content. In two-dimensional gel electrophoresis, they have one different isoform at pI lower than 5.77. However, highly different elution profiles have been recorded following size-exclusion HPLC. The use of a I-125 silica gel column has thus provided convincing evidence that both proteins do not show exactly the same hydrophobic three-dimensional structure.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D012584:Sciatic Nerve / Q000032:analysis; D014168:Transferrin / Q000302:isolation & purification*
D009419:Nerve Tissue Proteins; D014168:Transferrin; C026913:sciatin