Chemotactic reactivity of eosinophils obtained from bone marrow and peritoneal cavity of cyclophosphamide-treated, Toxocara canis-infected mice.
Chemotactic reactivity of eosinophils obtained from the bone marrow (BM-Eo) of cyclophosphamide-treated, Toxocara canis-infected mice was compared to that of eosinophils obtained from the peritoneal cavity (PEC-Eo). BM-Eo responded well to alanyl-tetrapeptide, a synthetic eosinophil chemotactic factor of anaphylaxis (ECF-A), whereas PEC-Eo did not. Both BM-Eo and PEC-Eo showed almost equally high chemotactic reactivity to parasite-derived ECF, ECF lymphokine and complement-derived ECF. Chemotactic reactivity of BM-Eo to synthetic ECF-A was deactivated by preincubation with ECF-A. Unresponsiveness of PEC-Eo to synthetic ECF-A could be explained by chemotactic deactivation by ECF-A, because an ECF-A-like substance was detected in the ascitic fluid; this substance could deactivate the chemotactic reactivity of BM-Eo to synthetic ECF-A. From these results, BM-Eo are naive and seem to be a good indicator for eosinophilotaxis and its modulation.
D000818:Animals; D001196:Ascariasis / Q000276:immunology*; D001854:Bone Marrow Cells; D002631:Chemotactic Factors, Eosinophil / Q000494:pharmacology / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D003165:Complement System Proteins / Q000502:physiology; D003520:Cyclophosphamide / Q000494:pharmacology*; D004804:Eosinophils / Q000276:immunology; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D010271:Parasites / Q000502:physiology; D010529:Peritoneal Cavity / Q000166:cytology; D014120:Toxocariasis / Q000276:immunology*
D002631:Chemotactic Factors, Eosinophil; D008222:Lymphokines; D003520:Cyclophosphamide; D003165:Complement System Proteins