Changes in bovine milk secretion following intramammary infusions of concanavalin A, oyster glycogen, or water.
Effects of sterile intramammary infusion of Concanavalin A on milk secretion were contrasted with infusion of oyster glycogen or water. Twenty-four cows were infused intramammary with 100 mg Concanavalin A, oyster glycogen in 20 ml water, or with 20 ml water alone. Concentrations of lactose, somatic cells, immunoglobulins G and A, serum albumin, and activity of N-acetyl-B-D-glucosaminidase were determined in milk. Blood N-acetyl-B-D-glucosaminidase activity and concentrations of blood immunoglobulins G and A and serum albumin were determined. Oyster glycogen and concanavalin A caused inflammation in treated quarters; peak elevations of milk somatic cell counts, serum albumin, immunoglobulin G concentrations, and N-acetyl-B-D-glucosaminidase activity were at 12 to 36 h following treatment. Milk production and lactose concentration were reduced by oyster glycogen and Concanavalin A. Selective indices of relative accumulation of milk immunoglobulins decreased following Concanavalin A and oyster glycogen, whereas the N-acetyl-B-D-glucosaminidase activity selective index generally remained unchanged. Inflammation reduced the selective accumulation of immunoglobulins, and absence of change in the N-acetyl-B-D-glucosaminidase selective index indicated that blood is not a major source of milk N-acetyl-B-D-glucosaminidase.
D000118:Acetylglucosaminidase / Q000032:analysis; D000818:Animals; D002417:Cattle / Q000502:physiology*; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D006003:Glycogen / Q000494:pharmacology*; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007263:Infusions, Parenteral; D007774:Lactation / Q000187:drug effects*; D007785:Lactose / Q000032:analysis; D008321:Mammary Glands, Animal / Q000187:drug effects*; D008892:Milk / Q000032:analysis* / Q000201:enzymology; D011247:Pregnancy; D012709:Serum Albumin / Q000032:analysis
D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D012709:Serum Albumin; D003208:Concanavalin A; D006003:Glycogen; D000118:Acetylglucosaminidase; D007785:Lactose