Mechanisms of petroleum hydrocarbon toxicity: functional changes in rat liver mitochondria after exposure to a Prudhoe Bay Crude Oil.
Administration of a Prudhoe Bay Crude Oil (PBCO) to rats (5 ml/kg/day for 2 days) resulted in a 30-35% inhibition of liver mitochondrial state 3 respiration with beta-hydroxybutyrate or succinate as substrates. beta-Hydroxybutyrate dehydrogenase, succinate dehydrogenase, NADH oxidase and succinate oxidase activities were also decreased in PBCO-treated rats. A significant increase in latent ATPase activity was also observed. Our results suggest that PBCO exerts its effects on liver mitochondria through the inhibition of beta-hydroxybutyrate- and succinate-supported electron transfer activities and by impairment of the mitochondrial membrane.
D000818:Animals; D006884:Hydroxybutyrate Dehydrogenase / Q000037:antagonists & inhibitors; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D010578:Petroleum / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors
D010578:Petroleum; D006884:Hydroxybutyrate Dehydrogenase; D013385:Succinate Dehydrogenase