Length and sequence polymorphisms in the ribosomal gene spacer of the Hawaiian sea urchin, T. gratilla.
Blot-hybridization of sea urchin (Tripneustes gratilla) genomic DNA with a cloned rDNA probe revealed individual variation in the length of the rDNA repeat unit and also in the non-transcribed spacer sequences. The number of distinct rDNA repeat subclasses distinguishable within any one sea urchin was limited and usually 2 to 3. However, examination of a number of sea urchins indicated a large number of distinct rDNA repeat types in the population as a whole; all of the rDNA repeat types in nine individuals were different. The presence of limited heterogeneity in the rDNA repeats of single individuals, with may different repeat types in the population as a whole, suggests that rDNA variants can be rapidly and selectively propagated within a chromosomal lineage.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005796:Genes; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012270:Ribosomes / Q000378:metabolism*; D012617:Sea Urchins / Q000235:genetics*; D014158:Transcription, Genetic
D004247:DNA