Differences of nucleoproteins of human and avian influenza A virus strains shown by polyacrylamide gel electrophoresis and by the peptide mapping technique.
Electrophoretic mobility differences in polyacrylamide gels were detected between (35S)-methionine-labelled nucleoproteins (NPs) induced in monolayer cells by 15 human and 4 avian reference strains of influenza viruses. The (35S)-methionine-labelled tryptic peptides of nucleoproteins of these strains were also analyzed by peptide mapping technique. Based on several detectable hydrophilic peptides the NPs could be arranged in 7 clearly differentiable groups. After radioiodination of NPs from 4 human and 3 avian reference strains the tryptic peptide patterns showed one clear difference between human and avian strains.
D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D009980:Influenza A virus / Q000032:analysis* / Q000235:genetics; D009698:Nucleoproteins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*
D009698:Nucleoproteins; D010446:Peptide Fragments; D014764:Viral Proteins