Simultaneous determination of serum uremic toxins, cations and anions and urea determination by post-column colorimetry using immobilized enzyme.
A column-switching method which combines ion-exchange and ion-pair reversed-phase chromatography was developed for the determination of serum uremic toxins, cations and anions. Serum urea, which was poorly separated by reversed-phase chromatography, was analyzed using an immobilized-urease column and detected by post-column colorimetry. Apart from the simultaneous analysis, anion analysis using ion-pair reversed-phase chromatography on an ODS column was also developed. The origin of the system peak observed in anion analysis with an eluent containing phthalate has been clarified.
D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D003124:Colorimetry; D004800:Enzymes, Immobilized; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D014118:Toxins, Biological / Q000097:blood*; D014508:Urea / Q000097:blood*; D014511:Uremia / Q000097:blood*
D004800:Enzymes, Immobilized; D014118:Toxins, Biological; C026865:uremia middle molecule toxins; D014508:Urea