[Capacity of the polynucleotide phosphorylase-releasing factor to stimulate hybrid cell growth].
The capacity of the partially purified preparation of the polynucleotide phosphorylase releasing factor (PNP-ase RF) from Walker sarcoma for stimulating the growth of hybrid cells and parent myeloma cells has been studied. The preparation of PNP-ase RF at a concentration of 500-50 micrograms/ml has been shown to induce the 3- to 10-fold increase of the number of cells in the culture by the 5th day of incubation without changing the medium.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000494:pharmacology*; D006822:Hybrid Cells / Q000187:drug effects*; D006825:Hybridomas / Q000187:drug effects; D008099:Liver; D008722:Methods; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000503:physiopathology; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism*; D051381:Rats; D012513:Sarcoma, Experimental / Q000378:metabolism; D013268:Stimulation, Chemical; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology
D006023:Glycoproteins; D009363:Neoplasm Proteins; D014020:Tissue Extracts; D011117:Polyribonucleotide Nucleotidyltransferase
Izuchenie sposobnosti faktora sniatiia polinukleotidfosforilazy stimulirovat' rost kletochnykh gibridov.