DMO-transport in human red blood cells.
The DMO (5,5-dimethyloxazolidine-2,4-dione) transport was studied in human red blood cells by measuring the 14C-DMO back exchange under self exchange conditions from 14C-DMO labeled cells at 0 degrees C. the unidirectional DMO-flux was linearly related to the DMO concentration up to approx. 100 mM. The unidirectional DMO-flux increases as pH is reduced. The DMO transport is not inhibited by anion transport inhibitors like DIDS, SITS, dipyridamole, phlorhizin, salicylate or butanol. A close correlation between the unidirectional DMO-flux and DMOH, the unionized form of DMO, has been observed suggesting that DMO is transported predominantly by nonionic diffusion. the permeability of DMOH is 9.5 X 10(-6) cm/s (0 degrees C) while the permeability of DMO- cannot be assessed from our data.
D000328:Adult; D001692:Biological Transport; D004114:Dimethadione / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008433:Mathematics; D008954:Models, Biological; D010080:Oxazoles / Q000097:blood*
D010080:Oxazoles; D004114:Dimethadione