[Effect of levorin and its combination with a modifying factor on lipid peroxidation in Crithidia oncopelti].
It was shown earlier by the authors that in the presence of sodium acetate as a modifier of C. oncopelti reaction to levorin, a polyenic antibiotic sensitivity of this organism to the antibiotic increased. At the same time there were observed changes in the ratio of the lipids and fatty acids of the total lipid fraction of C. oncopelti. The changes in the lipid composition of the biological membranes influenced lipid peroxidation (LPO). The present paper deals with investigation of interrelation between increasing of C. oncopelti sensitivity to levorin in the presence of sodium acetate and LPO levels. Accumulation of the primary, secondary and final LPO products in C. oncopelti under the effect of sodium acetate, levorin (1 microgram/ml) added simultaneously with the modifier and levorin in a concentration of 10 micrograms/ml was investigated. It was shown that sodium acetate did not induce accumulation of LPO products as compared to the initial culture. Levorin added simultaneously with sodium acetate and levorin in a concentration of 10 micrograms/ml induced an increase in the content of LPO products in the C. oncopelti lipids. The causes of the LPO rate increase in the presence of the polyenic antibiotic are discussed.
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000935:Antifungal Agents / Q000494:pharmacology*; D002174:Candicidin / Q000494:pharmacology*; D003421:Crithidia / Q000187:drug effects* / Q000378:metabolism; D004347:Drug Interactions; D008054:Lipid Peroxides / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D013997:Time Factors
D000085:Acetates; D000935:Antifungal Agents; D008054:Lipid Peroxides; D002174:Candicidin; D008315:Malondialdehyde; D019342:Acetic Acid
Vliianie levorina i ego sochetaniia s modifitsiruiushchim faktorom na perekisnoe okislenie lipidov u Crithidia oncopelti.