Preparation of astrocyte-deficient cultures containing neurons and oligodendroglia from embryonic rat cerebral hemispheres.
Complement-dependent anti-glial fibrillary acidic protein (GFAP) antibody-mediated cytotoxicity was utilized in order to prepare astrocyte-deficient cultures from mechanically dissociated cells from 18-day-old embryonic rat cerebral hemispheres. Neurons and a lesser number of oligodendroglia were the major cell components in such cultures.
D000818:Animals; D001253:Astrocytes / Q000032:analysis / Q000166:cytology*; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000032:analysis / Q000166:cytology*; D046508:Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000032:analysis / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains
D005904:Glial Fibrillary Acidic Protein