title: Action of ketotifen on different functions of neutrophil polymorphonuclear cells.
abstract: The results concerning the action of Ketotifen "in vitro" on the adherence, spontaneous and chemotactic mobility and phagocytosis of neutrophil polymorphonuclear cells (PMNs) of the non-specific immunological system are presented. PMN cells were incubated for 30 min at 37 degrees C prior to the evaluation of different functions, with the following concentrations of Ketotifen: 1, 10 and 100 ng, and 1 and 10 micrograms/ml., final concentration. PMN cell adherence to glass spheres was unaffected by doses of the drug considered to have pharmacological action; however, a statistically significant enhancement was observed in this function from a concentration of 10 ng./ml. onwards. Mobility, both spontaneous and chemotactic was inhibited in the presence of Ketotifen only at high doses; the chemotactic function was more susceptible to the action of the drug at doses of 10 ng./ml. than spontaneous mobility, which required ten-fold greater concentrations for inhibition to take place. Likewise, the phagocytosis of C. albicans was inhibited by the presence of the drug at doses of 10 ng./ml. and higher. In conclusion, Ketotifen in PMN cells at pharmacological doses only induces a slight increase in adherence and a decrease in the functions of mobility and phagocytosis of C. albicans; at least "in vitro", much higher concentrations, which are not found with the doses usually employed, are required for these functions to be altered significantly.
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006801:Humans; D007665:Ketotifen / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects
chemical_list: D007665:Ketotifen