Selection of a mouse embryonal carcinoma clone resistant to the inhibition of metabolic cooperation by retinoic acid.
A novel selection for cell lines resistant to the blocking of metabolic cooperation by chemical inhibitors is described. The selection has been used to isolate a pluripotent embryonal carcinoma clone which cooperates in retinoic acid (RA), an inhibitor of junctional communication in all other cell lines tested to date. The selected cells require higher levels of RA to induce differentiation, but show little or no increase in resistance to toxic effects of RA.
D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004351:Drug Resistance; D051379:Mice; D012641:Selection, Genetic; D013724:Teratoma / Q000378:metabolism* / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology*; D014500:Uracil Nucleotides / Q000378:metabolism
D014500:Uracil Nucleotides; D014212:Tretinoin