Fusion of Newcastle disease virus with liposomes: role of the lipid composition of liposomes.
We demonstrate here that fusion occurs between the membrane of the Newcastle disease virus (NDV) and liposomes. Fluorescence dequenching studies (using Rhodamine-bearing viral envelopes) revealed the mixing of the lipids constituting the viral and liposomal membrane. The digestion of internal viral proteins by trypsin-containing liposomes indicated the mixing of the internal aqueous compartments. This last assay is independent of exchange of lipids between liposomal and viral membrane in the absence of fusion. Investigation of the effects of liposomal composition indicated that the presence of phosphatidylethanolamine and gangliosides are essential to optimize fusion. The fact that the Newcastle disease virus membrane can fuse with liposome also confirms that fusion must be determined by the viral proteins and could be mostly independent of the nature or presence of the host proteins.
D002784:Cholesterol / Q000502:physiology; D005456:Fluorescent Dyes; D005732:Gangliosides / Q000502:physiology; D008081:Liposomes; D008561:Membrane Fusion; D008563:Membrane Lipids / Q000502:physiology*; D009522:Newcastle disease virus / Q000502:physiology*; D010713:Phosphatidylcholines / Q000502:physiology; D010714:Phosphatidylethanolamines / Q000502:physiology; D012235:Rhodamines; D013050:Spectrometry, Fluorescence; D013109:Sphingomyelins / Q000502:physiology
D005456:Fluorescent Dyes; D005732:Gangliosides; D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D012235:Rhodamines; D013109:Sphingomyelins; D002784:Cholesterol