Use of the biotin-avidin system for detecting a broad range of serologically related plant viruses by ELISA.
The biotin-avidin detection system was used in direct and indirect ELISA for detecting a broad range of serologically related tobamoviruses. When compared to standard ELISA procedures that use antibodies labelled with alkaline phosphatase, the biotin-avidin system increased the assay sensitivity and allowed a wider range of related viral serotypes to be detected.
D000469:Alkaline Phosphatase; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D009029:Mosaic Viruses / Q000276:immunology / Q000302:isolation & purification*; D010047:Ovalbumin / Q000031:analogs & derivatives; D013045:Species Specificity; D014027:Tobacco Mosaic Virus / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin; D000469:Alkaline Phosphatase