A sensitive ELISA for the quantitation of human C5a in blood plasma using a monoclonal antibody.
An ELISA for the quantitation of the C5a anaphylatoxin of complement in human plasma is presented which is based on the use of both a monoclonal antibody and polyclonal antibodies to C5a. Its detection limit is 1 ng C5a/ml plasma. It detects no C5a in fresh EDTA-plasma. Complete removal of C5 by an optimized precipitation step prior to the assay procedure is an essential feature of the method.
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001331:Automation; D003101:Collodion; D003167:Complement Activation / Q000187:drug effects; D003182:Complement C5 / Q000032:analysis* / Q000302:isolation & purification; D015936:Complement C5a; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007700:Kinetics; D010209:Paper; D015054:Zymosan / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D003182:Complement C5; D015936:Complement C5a; D003101:Collodion; D015054:Zymosan