title: Formation of triazene from sulphonamide and nitrite in acidic solution.
abstract: Formation of triazene derivatives from sodium nitrite and sulphanilamide, sulphamethizole or sulphamethoxazole in acidic solution containing saliva or canned vegetable juice was studied using reverse-phase high-performance liquid chromatography with ultraviolet light detection at 365 nm. At the optimal pH of 3.2-3.7, the mean yield of the triazene formed by reaction of sulphanilamide and nitrite in saliva was 28.8%, calculated on the nitrite content, and there was a high positive correlation (r = 0.993) between the nitrite concentration in the saliva and the triazene yield. In contrast, at the optimal pH values for triazene formation from sulphamethizole and sulphamethoxazole (3.0-3.5 and 2.5-2.9 respectively), triazene yields were much lower, accounting for only 0.9 and 1.0%, respectively, of the nitrite present. Triazene formation was partly inhibited in the presence of vegetable juice.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009573:Nitrites; D012463:Saliva; D012977:Sodium Nitrite; D013419:Sulfamethizole; D013420:Sulfamethoxazole; D000077145:Sulfanilamide; D013424:Sulfanilamides; D014227:Triazines / Q000138:chemical synthesis*; D014675:Vegetables
chemical_list: D009573:Nitrites; D013424:Sulfanilamides; D014227:Triazines; D000077145:Sulfanilamide; D013419:Sulfamethizole; D013420:Sulfamethoxazole; D012977:Sodium Nitrite