title: Pulmonary response of hamsters to fibrous glass. Clearance and morphology after a single intratracheal instillation.
abstract: In a one-year serial sacrifice study 10 mg glass fibres (length, 95% alpha 20 microns, 89% alpha 12 microns, 58% alpha 5 microns and 25% alpha 2 microns; diameter, 88% alpha 1.0 micron, 60% alpha 0.5 micron and 31% alpha 0.25 micron) suspended in 0.2 ml saline solution were administered to Syrian golden hamsters by a single intratracheal instillation to determine the clearance of the glass fibres from the lungs and to examine their effects on the lungs using light and electron microscopy. The clearance was rather efficient with a half-time of about 3 months. Coating and corrosion of glass fibres were sporadic findings. A violent focal acute pneumonitis was evoked by the glass fibres and was followed by excessive accumulations of alveolar macrophages often loaded with glass fibres. Thereafter, "silicotic granulomas" developed which were seen as clusters of tightly packed iron-positive macrophages containing glass fibres. These granulomas were surrounded by a layer of alveolar epithelium, and were by the end of the study the predominant lesion in otherwise normal lungs.
mesh_terms: D000818:Animals; D006224:Cricetinae; D005260:Female; D005898:Glass; D006099:Granuloma / Q000209:etiology; D006207:Half-Life; D008168:Lung / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D008854:Microscopy, Electron; D009504:Neutrophils / Q000378:metabolism; D010587:Phagocytosis; D011014:Pneumonia / Q000209:etiology
chemical_list: C009753:fiberglass