A novel approach for obtaining and identifying monoclonal antibodies that react with differentiation-specific antigens using human hybrid cells.
HeLa X keratinocyte hybrid cells have been used as immunogens to generate monoclonal antibodies against differentiation-specific antigens. A key feature of the experimental system is that the hybrid cells are undifferentiated in culture and yet terminally differentiate in the mouse. The properties of these cells allow one a facile approach to obtaining and characterizing monoclonal antibodies against differentiation-specific antigens. Use of hybrid cells derived from HeLa X differentiated cell fusions as immunogens should prove to be a general method for identifying differentiation-specific antigens from a variety of differentiated cell types.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology*; D002454:Cell Differentiation; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology*; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface