Rat liver microsomal phospholipase A2 and membrane fluidity.
The influence of the physical state and the lipid composition on microsomal membrane-bound phospholipase A2 activity has been investigated. It was established that the decrease of membrane fluidity expressed by the alterations of the steady-state fluorescent anisotropy (rs) and the structural order parameter for DPH (SDPH) leads to augmentation of phospholipase A2 specific activity. Phosphatidylinositol is the only phospholipid having a specific activating effect on microsomal membrane-bound phospholipase A2.
D000818:Animals; D007425:Intracellular Membranes / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis; D008862:Microsomes, Liver / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D008563:Membrane Lipids; D010743:Phospholipids; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2