[3H]cyclohexyladenosine binding in rat brain: a pharmacological analysis using quantitative autoradiography.
Quantitative autoradiography was used to characterize the regional binding parameters of [3H]cyclohexyladenosine (CHA) and the regional inhibition of specific [3H]CHA binding observed with a series of adenosine agonists and antagonists. Under the conditions described [3H]CHA binding was best fit by a single, high affinity site model in all areas examined. No major regional differences were observed in the inhibition of [3H]CHA binding by the adenosine and xanthine analogs examined. Overall, the rank order of activity throughout all regions was CPA greater than R-PIA greater than NECA much greater than PACPX greater than or equal to 2-CADO greater than or equal to S-PIA much greater than DPX.
D000241:Adenosine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D008297:Male; D008954:Models, Biological; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014316:Tritium; D014970:Xanthines / Q000494:pharmacology
D014970:Xanthines; D014316:Tritium; C027513:N(6)-cyclohexyladenosine; D000241:Adenosine