[Modification of monoclonal antibodies by polymers possessing chelating properties].
Monoclonal antibodies to heavy chains of the heart myosin were chemically modified by chelate polymers containing EDTA or DTPA residues. The modification allows binding up to 90 atoms of heavy metals (e.g. 111In, Mn2+, Cd3+) per protein globule, i.e. an increase in the specific activity of labeling 20 to 50-fold in comparison with previous methods. Specific affinity of the modified protein is preserved. Conjugates obtained may be useful for in vivo immuno-imaging.
D000818:Animals; D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D006801:Humans; D051379:Mice; D009206:Myocardium / Q000276:immunology; D009218:Myosins / Q000276:immunology; D011094:Polyethyleneimine; D011107:Polylysine; D011108:Polymers
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D011108:Polymers; D011107:Polylysine; D011094:Polyethyleneimine; D009218:Myosins
Modifikatsiia monoklonal'nykh antitel polimerami, obladaiushchimi khelatiruiushchimi svoĭstvami.