Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions.
Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysulfosuccinimide to such reactions can greatly enhance the yields obtained.
D002234:Carbodiimides; D005998:Glycine; D006433:Hemocyanins; D012995:Solubility; D013388:Succinimides; D014867:Water
D002234:Carbodiimides; D013388:Succinimides; D014867:Water; C035761:N-hydroxysulfosuccinimide; D006433:Hemocyanins; D005998:Glycine