Trypanosoma brucei: cis-aconitate and temperature reduction as triggers of synchronous transformation of bloodstream to procyclic trypomastigotes in vitro.
Synchronous transformation of the monomorphic Trypanosoma brucei 427 variant clone MITat 1.4 (117) from bloodstream to procyclic trypomastigotes was studied in modified minimum essential medium plus 15% inactivated horse serum. Repression of variant surface glycoprotein synthesis, subsequent morphological transformation, and growth of procyclic cells was triggered by the simultaneous action of two signals: a reduction in temperature from 37 to 27 C and the addition of cis-aconitate. Repression of variant surface glycoprotein synthesis initiated by these two signals is reversible during the first hours, but becomes irreversible after about 1 day. Thereafter, cells are committed to differentiation at 27 C.
D000156:Aconitic Acid / Q000494:pharmacology*; D000818:Animals; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D003470:Culture Media; D006023:Glycoproteins / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis; D013696:Temperature; D014233:Tricarboxylic Acids / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D014643:Variant Surface Glycoproteins, Trypanosoma
D002951:Citrates; D003470:Culture Media; D006023:Glycoproteins; D008565:Membrane Proteins; D014233:Tricarboxylic Acids; D014643:Variant Surface Glycoproteins, Trypanosoma; D019343:Citric Acid; D000156:Aconitic Acid