Cerebral endothelial GABA-T activity: effects of in vivo GABA-T inhibition.
The effects of gamma-aminobutyric acid transaminase (GABA-T) inhibitors on rat endothelial GABA-T activity has been studied utilizing a modified tetrazolium salt histochemical technique. Blue-diformazan staining of cerebrovascular and parenchymal sites of GABA catabolism in normal brain was almost completely prevented in unfixed frozen brain sections from rats subjected to 'in vivo' GABA-T inhibition using aminooxyacetic acid or gabaculine. These results provide histochemical evidence for the efficacy of using systemically administered GABA-T inhibitors to inhibit endothelial GABA-T activity.
D000612:4-Aminobutyrate Transaminase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000085:Acetates / Q000494:pharmacology*; D000625:Aminooxyacetic Acid / Q000494:pharmacology*; D000818:Animals; D001808:Blood Vessels / Q000201:enzymology*; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply* / Q000201:enzymology; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D004727:Endothelium / Q000201:enzymology; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D003509:Cyclohexanecarboxylic Acids; D000625:Aminooxyacetic Acid; C012987:gabaculine; D000612:4-Aminobutyrate Transaminase