title: Proximal tubular atrophy: qualitative and quantitative structural changes in chronic obstructive nephropathy in the pig.
abstract: Kidneys of pigs with various degrees of induced chronic obstructive nephropathy were studied by light- and electron microscopy to assess the structural changes of proximal convoluted tubules with increasing degrees of atrophy. A particular aim was to evaluate the quantitative relationship between proximal tubular and interstitial changes in early tubular atrophy. The kidneys were subjected to varying degrees of ureteral obstruction and were fixed by in vivo vascular perfusion. Quantitative (morphometric) analyses were carried out on montages of electron micrographs representing randomly selected cortical areas and cross sections of individual proximal convoluted tubules. The results demonstrated that ureteral obstruction was followed by significant reductions in proximal tubular epithelium, in volume of proximal tubular mitochondria and in surface area of proximal tubular basolateral membranes. These changes were present even in the absence of any demonstrable increase in cortical interstitium or alterations in the relationships between proximal tubules and peritubular capillaries. With increase in the volume of cortical interstitium the proximal tubules were further simplified in ultrastructure with a reduced number of interdigitating lateral cell processes. Concomitantly there were significant quantitative changes in the spatial associations between tubules and capillaries due to increase in tubulo-capillary distances. The present study shows that ultrastructural changes in proximal tubules during early atrophy precede the volume increase in cortical interstitium associated with chronic obstructive nephropathy. It is suggested that the early tubular changes are due to decreased functional loads, whereas the further progression of tubular atrophy may be a result of impaired nourishment of the tubular cells due to increased interstitial tissue and altered relationships between tubules and capillaries.
mesh_terms: D000818:Animals; D001284:Atrophy; D002908:Chronic Disease; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D007674:Kidney Diseases / Q000473:pathology*; D007687:Kidney Tubules, Proximal / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009399:Nephrons / Q000473:pathology / Q000648:ultrastructure; D013552:Swine; D014517:Ureteral Obstruction / Q000473:pathology