title: Multiplication of maize rayado fino virus in the leafhopper vector Dalbulus maidis.
abstract: The enzyme-linked immunosorbent assay (ELISA) was used to demonstrate the increase in titer of maize rayado fino virus (MRFV) in its leafhopper vector, Dalbulus maidis. Viral antigen concentration attained a maximum in the body of the insect 25 days after virus acquisition and decreased thereafter. Substantial differences in concentration were observed among viruliferous leafhoppers. MRFV was serially passed through 5 successive leafhopper populations. The results provide further evidence of multiplication of MRFV in D. maidis.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006430:Hemiptera / Q000382:microbiology*; D010942:Plant Viruses / Q000254:growth & development* / Q000276:immunology; D003313:Zea mays / Q000382:microbiology
chemical_list: D000956:Antigens, Viral