Establishment of passive hemagglutination assay (PHA) system for anti-HBc in plasma.
A sensitive and specific assay system for anti-HBc, the passive hemagglutination (PHA) method, has been established. The reactivity of PHA cells prepared by conjugating purified recombinant HBcAg-particles with fixed sheep blood cells (SRBC) was highly specific to monoclonal- and polyclonal anti-HBc IgGs. The sensitivity of PHA method was higher than that of radioimmunoassay (RIA). However, uncertainty for the positivity of anti-HBc still remained in plasma with the PHA titers lower than 2(5). A relatively high ratio (19%, 37 of 196) of anti-HBc-positive plasma, which had been confirmed to be HBsAg negative, was demonstrated in blood donors whose bloods have been considered suitable for transfusion. The hazards of anti-HBc-positive bloods and the importance of anti-HBc detection in plasma are discussed in this paper.
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D006386:Hemagglutination Tests; D006509:Hepatitis B / Q000175:diagnosis / Q000276:immunology* / Q000517:prevention & control; D006512:Hepatitis B Core Antigens / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006801:Humans; D011863:Radioimmunoassay; D065227:Transfusion Reaction
D000911:Antibodies, Monoclonal; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens