Sequential sampling and analysis of renal hydroxylase activities of cattle given 1 alpha-hydroxyvitamin D3.
A new method was developed for sequential sampling of bovine renal cortex. This method results in minimum hemorrhage and adhesions and provides sufficient renal cortex tissue for assay of 25-hydroxyvitamin D 1 alpha-, 24-, and 23-hydroxylase activities. Application of this procedure in calves and pregnant cows treated with 1 alpha-hydroxyvitamin D3 is described. The success of these experiments suggests these techniques could be used to follow enzyme activities that control crucial aspects of vitamin D metabolism in normal peripartum cows and cows with milk fever or other diseases of mineral metabolism.
D000818:Animals; D001706:Biopsy / Q000295:instrumentation / Q000662:veterinary; D002417:Cattle / Q000378:metabolism*; D053493:Cholestanetriol 26-Monooxygenase; D003577:Cytochrome P-450 Enzyme System; D005260:Female; D006887:Hydroxycholecalciferols / Q000008:administration & dosage / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology*; D011247:Pregnancy; D013250:Steroid Hydroxylases / Q000378:metabolism*; D065668:Vitamin D3 24-Hydroxylase
D006887:Hydroxycholecalciferols; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D053493:Cholestanetriol 26-Monooxygenase; D065668:Vitamin D3 24-Hydroxylase