Thyroxine 5'-deiodinase in brown adipose tissue of myopathic hamsters.
Myopathic Syrian hamsters (BIO 14.6) have less brown adipose tissue (BAT) than normal. The trophic response of this tissue to cold is smaller than normal and trophic responses to diet and to photoperiod are absent. The objective was to find out whether activity of thyroxine 5'-deiodinase in their BAT was increased normally in response to cold and thus whether a defect in endogenous production of 3,5,3'-triiodothyronine might underlie the attenuated trophic response. The effect of feeding a high-fat diet on activity of 5'-deiodinase was also studied. Cold acclimation increased thyroxine 5'-deiodinase activity in BAT of the myopathic hamster, but the total remained smaller than normal because of the smaller size. The cold-induced increase in concentration of mitochondrial uncoupling protein was also smaller than normal. The level of serum 3,5,3'-triiodothyronine was low in myopathic hamsters and remained lower than normal when they were cold-exposed or cold acclimated. Feeding the high-fat diet to myopathic hamsters resulted in a greater than normal suppression of thyroxine 5'-deiodinase activity than in normal hamsters; the normal increases in protein content and in concentration of mitochondrial uncoupling protein were absent. We conclude that the defective trophic response of BAT of the myopathic hamster is not secondary to defective regulation of its thyroxine 5'-deiodinase activity because this activity does not appear to be obligatorily linked to hypertrophy of BAT. The low level of serum 3,5,3'-triiodothyronine in the myopathic hamster may be secondary to reduced capacity for peripheral thyroxine deiodination in its BAT.
D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000201:enzymology* / Q000502:physiology; D000818:Animals; D001833:Body Temperature Regulation; D001835:Body Weight; D002352:Carrier Proteins; D003080:Cold Temperature; D006224:Cricetinae; D004041:Dietary Fats / Q000494:pharmacology; D006153:Guanosine Diphosphate / Q000378:metabolism; D007453:Iodide Peroxidase / Q000378:metabolism*; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008647:Mesocricetus; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D009137:Muscular Dystrophy, Animal / Q000201:enzymology*; D014284:Triiodothyronine / Q000378:metabolism*; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D004041:Dietary Fats; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1; D014284:Triiodothyronine; D006153:Guanosine Diphosphate; D007453:Iodide Peroxidase