A semi-automated fluorimetric method for measuring acetylcholinesterase activity in small volumes of cerebrospinal fluid and tissue extracts using acetylcholine as substrate.
A fluorimetric method for the assay of acetylcholinesterase (AChE; EC 3.1.1.7) in tissue extracts and cerebrospinal fluid, using acetylcholine as the substrate, is described. The method is based on the measurement of hydrogen peroxide, formed by the oxidation of choline resulting from the action of AChE on acetylcholine, by means of horseradish peroxidase and 4-hydroxy-3-methoxyphenylacetic acid (HVA) to yield a fluorescent derivative. Choline, if present in any sample to be analysed, is first removed by a modification of the peroxidase reaction during preincubation.
D000109:Acetylcholine; D000110:Acetylcholinesterase / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D007700:Kinetics; D008297:Male; D010902:Pituitary Gland / Q000201:enzymology; D051381:Rats; D013050:Spectrometry, Fluorescence
D000110:Acetylcholinesterase; D000109:Acetylcholine