title: [Adsorbed subunit influenza vaccine: its isolation and characteristics].
abstract: Experimental batches of adsorbed subunit influenza vaccine were prepared from the envelope of glycoprotein antigens separated from the influenza virion by treatment with a cationic detergent (cetyltrimethylammonium bromide). Purified and concentrated influenza virus strains A(H1N1) and A(H3N2) were obtained by gradient centrifugation; additional purification, with a view to removing ovalbumin and structural components of the chorioallantoic membrane, was achieved by gelfiltration. The composition and biological properties of the vaccine are described.
mesh_terms: D000327:Adsorption; D000818:Animals; D002642:Chick Embryo; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000276:immunology; D006168:Guinea Pigs; D006389:Hemagglutinins, Viral / Q000032:analysis; D009980:Influenza A virus / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology / Q000302:isolation & purification*; D008722:Methods; D051379:Mice; D014613:Vaccines, Attenuated / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000276:immunology; D014771:Virion / Q000276:immunology
chemical_list: D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D014764:Viral Proteins
vernacular_title: Adsorbirovannaia grippoznaia sub''edinichnaia (AGS)vaktsina: poluchenie i kharakteristika.