title: Calcium and calmodulin stimulated in vitro phosphorylation of rooster brain tubulin and MAP-2 following a single oral dose of tri-o-cresyl phosphate.
abstract: The effect of a single oral administration of 750 mg/kg tri-o-cresyl phosphate (TOCP) on endogenous phosphorylation of specific brain cytosolic proteins has been studied in roosters following the development of delayed neurotoxicity. In vitro phosphorylation assay using [gamma-32P]ATP was carried out. Proteins were then resolved on one dimensional 8% SDS-PAGE and two-dimensional gel electrophoresis, stained with Coomassie blue and autoradiographed. The amount of proteins, as well as the amount of 32P incorporation, were quantified by microdensitometry, TOCP-administration enhanced the phosphorylation of cytosolic proteins of M, 52-59 kDa, 70 kDa, and 300 kDa by as much as 155%, 199% and 166%, respectively. Two-dimensional gel electrophoresis confirmed the 52-59 kDa proteins as alpha and beta tubulin and the 300 kDa protein as microtubule-associated protein-2.
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D002645:Chickens; D003408:Cresols / Q000008:administration & dosage*; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009498:Neurotoxins / Q000008:administration & dosage; D010766:Phosphorylation; D014317:Tritolyl Phosphates / Q000008:administration & dosage*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D003408:Cresols; D008869:Microtubule-Associated Proteins; D009498:Neurotoxins; D014317:Tritolyl Phosphates; D014404:Tubulin; D002118:Calcium; C025541:tri-o-cresyl phosphate