Coupling of growth arrest and expression of early markers during adipose conversion of preadipocyte cell lines.
After growth arrest at the entry of the S phase of the cell cycle, Ob1771 and 3T3-F442A cells, but not 3T3-C2 cells, accumulate lipoprotein lipase and pOb24 mRNA that are early markers of adipose conversion. Removal of the single- or double-thymidine block when cultured cells are present at low density leads first to DNA synthesis and growth resumption, then to a continuous proliferation and a rapid disappearance of these markers. By contrast, growth-arrested Ob1771 cells reinoculated at high density undergo a single round of cell division, maintain high levels of early marker(s) and acquire with time both glycerol-3-phosphate dehydrogenase and lipids. Thus, depending upon the conditions in culture, growth-arrested cells can undergo either a dedifferentiation leading to a loss of early markers or a terminal differentiation leading to the acquisition of late markers.
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis; D008071:Lipoprotein Lipase / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D013936:Thymidine / Q000494:pharmacology; D013997:Time Factors
D012333:RNA, Messenger; D004247:DNA; D005993:Glycerolphosphate Dehydrogenase; D008071:Lipoprotein Lipase; D013936:Thymidine