Detecting Mycoplasma bovis in milk by enzyme-linked immunosorbent assay, using monoclonal antibodies.
Enzyme-linked immunosorbent assay, using monoclonal antibodies, was used to detect Mycoplasma bovis in milk samples from a dairy experiencing an epizootic of mastitis. This method was specific (100%) for M bovis. Broth enrichment increased the sensitivity from 65% to 86%, compared with standard culture methods.
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000276:immunology; D008892:Milk / Q000382:microbiology*; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial