[Quantitative gas chromatographic determination of 3-methylhistidine in biological fluids].
The gas chromatographic procedure is suggested to determine 3-methylhistidine in biological fluids. The amino acid fraction containing 3-methylhistidine is separated by ion-exchange chromatography. Amino acids are transformed into N-trifluoroacetyl-O-isobutyl esters which are analyzed by the gas chromatography instrument with micropacked columns and ionization-resonance detector. The limit of the quantitative determination of 3-methylhistidine is 50 ng per a probe.
D002849:Chromatography, Gas; D006639:Histidine / Q000031:analogs & derivatives*; D006801:Humans; D008762:Methylhistidines / Q000032:analysis* / Q000097:blood / Q000652:urine
D008762:Methylhistidines; C028120:1-methylhistidine; D006639:Histidine; C028118:3-methylhistidine
Kolichestvennyĭ gazokhromatograficheskiĭ analiz 3-metilgistidina v biologicheskikh zhidkostiakh.