title: N-hydroxy-2-acetylaminofluorene as effective inhibitor of aldehyde oxidase.
abstract: N-Hydroxy-2-acetylaminofluorene has been found to be an effective inhibitor of aldehyde oxidase. At concentrations of 1 X 10(-6) M and 1 X 10(-5) M, 38% and 88% inhibition was observed on the oxidase activity towards N1-methylnicotinamide. The inhibition was of noncompetitive type and had a Ki value of 4.4 X 10(-6) M. In contrast, little inhibition of the enzyme was observed with 2-aminofluorene, 2-acetylaminofluorene and acetohydroxamic acid even at a concentration of 1 X 10(-4) M.
mesh_terms: D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives* / Q000494:pharmacology; D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors*; D000611:Aminobiphenyl Compounds / Q000494:pharmacology; D000818:Animals; D003545:Cysteine / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005449:Fluorenes / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology; D006881:Hydroxyacetylaminofluorene / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D010615:Phenacetin / Q000031:analogs & derivatives / Q000494:pharmacology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D000611:Aminobiphenyl Compounds; D005449:Fluorenes; D006877:Hydroxamic Acids; C008068:N-hydroxyphenacetin; D012710:Serum Albumin, Bovine; C012177:2-aminofluorene; C001365:N-hydroxy-4-acetylaminobiphenyl; C006358:acetohydroxamic acid; D006881:Hydroxyacetylaminofluorene; D015073:2-Acetylaminofluorene; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase; D010615:Phenacetin; D005978:Glutathione; D003545:Cysteine; D004229:Dithiothreitol