Intestinal retinol esterification and serum retinol in children with cystic fibrosis.
Children with cystic fibrosis (CF) often have a poor vitamin A status. We found in the present work a reduced level of serum retinol in older children with this disease. As retinol enters intestinal lymph as retinyl esters, the enzyme acyl-CoA:retinol acyltransferase (ARAT) may be of importance for retinol absorption. We have assayed ARAT activity in duodenal mucosal homogenate from children with CF. There was a large variation within the group. However, mean ARAT activity was not significantly decreased, as compared with controls. Thus, the lack of pancreatic enzymes probably is the main reason for reduced vitamin A absorption in CF. In celiac disease and in lactose intolerance--both mucosal disorders--a significant reduction of mean ARAT activity was found.
D000217:Acyltransferases / Q000378:metabolism*; D000293:Adolescent; D002446:Celiac Disease / Q000201:enzymology; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000201:enzymology*; D004386:Duodenum / Q000201:enzymology; D004951:Esterification; D005260:Female; D006801:Humans; D007223:Infant; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000201:enzymology*; D007787:Lactose Intolerance / Q000201:enzymology; D008297:Male; D051142:Retinol O-Fatty-Acyltransferase; D012177:Retinol-Binding Proteins / Q000378:metabolism; D014801:Vitamin A / Q000097:blood* / Q000378:metabolism
D012177:Retinol-Binding Proteins; D014801:Vitamin A; D000217:Acyltransferases; D051142:Retinol O-Fatty-Acyltransferase