title: A human lymphokine released by mononuclear blood cells upon contact with CEA.
abstract: As revealed by the macrophage electrophoretic mobility (MEM) technique mononuclear blood cells from certain cancer patients respond to carcinoembryonic antigen (CEA). This phenomenon appeared to be due to a specific lymphokine release. In this study, the lymphokine activity of supernatant pools was stepwise enriched by gel filtration on Sephadex. The mediator activity was recovered within a molecular mass region less than 47 kDA. The lymphokine was highly enriched during further gel filtration steps and showed a single activity peak in the molecular mass region of 23.5 kDa. Gel filtrations of appropriate control supernatants resulted in biologically inactive fractions. The lymphokine was heat-labile at 56 degrees C, showed a clear-cut, dose-dependent effect on macrophages, and could be blocked by fucose. Preparative gel electrophoresis of radiolabeled and unlabeled lymphokine resulted in two corresponding peaks of biological activity and radioactivity.
mesh_terms: D002272:Carcinoembryonic Antigen / Q000276:immunology*; D002465:Cell Movement; D003110:Colonic Neoplasms / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000302:isolation & purification / Q000378:metabolism*; D008262:Macrophage Activation; D009000:Monocytes / Q000276:immunology*; D009005:Monosaccharides / Q000276:immunology; D012004:Rectal Neoplasms / Q000276:immunology
chemical_list: D002272:Carcinoembryonic Antigen; D008222:Lymphokines; D009005:Monosaccharides