title: Sequential cerebral blood flow study by chrono-autoradiography.
abstract: A novel chrono-autoradiographic technique (sequential double label autoradiography) was established in order to evaluate regional cerebral blood flow (CBF) changes utilizing N-isopropyl-p-123I-iodoamphetamine (123I-IMP) and 14C-iodoantipyrine (14C-IAP). With sequential administration of 123I-IMP and 14C-IAP, two CBF images were obtained from the same brain section. By applying this method to focal cerebral ischemia, two sequential CBF images, one during ischemia and one after recirculation, were obtained. These results show that this method is useful in topographical evaluation of CBF changes. From a pathophysiological point of view, the result demonstrates a heterogeneous tissue perfusion after a transient ischemic insult.
mesh_terms: D000662:Amphetamines; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives; D001345:Autoradiography / Q000379:methods; D002560:Cerebrovascular Circulation; D020367:Iofetamine; D002546:Ischemic Attack, Transient / Q000000981:diagnostic imaging*; D008297:Male; D011859:Radiography; D051381:Rats; D013997:Time Factors
chemical_list: D000662:Amphetamines; D020367:Iofetamine; D000983:Antipyrine; C008135:iodoantipyrine