Deoxyribonucleic acid synthesis in mesometrial arteries of guinea pigs during oestrous cycle, pregnancy and treatment with oestradiol benzoate.
Terminal portions of mesometrial arteries which supply the myometrium and the placenta, respectively, were excised at various stages of the ovarian cycle and pregnancy, as well as after oestrogen treatment, and incubated in solutions containing [3H]thymidine. The incorporation of radioactivity into the acid-insoluble material was measured in order to evaluate the actual rate of DNA synthesis before and during pregnancy and after oestrogen administration. We found that the rate of incorporation rises 30-fold before oestrus and 40-fold in the beginning of pregnancy. A 70-fold rise in incorporation was initiated by 10 micrograms oestradiol benzoate (administered subcutaneously) in dioestrous and ovariectomized animals. We conclude that similar peaks of DNA synthesis in mesometrial arteries occur during oestrus and at the beginning of pregnancy. Oestradiol is capable of inducing the observed surge of DNA synthesis. The results are in agreement with the concept that oestradiol is a chemical regulator of arterial growth during pregnancy.
D000818:Animals; D001158:Arteries / Q000033:anatomy & histology / Q000378:metabolism; D001345:Autoradiography; D004247:DNA / Q000096:biosynthesis*; D004031:Diestrus; D004958:Estradiol / Q000494:pharmacology*; D004971:Estrus; D005260:Female; D006168:Guinea Pigs; D007213:Indomethacin / Q000494:pharmacology; D009215:Myometrium / Q000098:blood supply*; D010052:Ovariectomy; D011247:Pregnancy; D011270:Pregnancy, Animal; D013629:Tamoxifen / Q000494:pharmacology; D013936:Thymidine
D013629:Tamoxifen; D004958:Estradiol; D004247:DNA; D013936:Thymidine; D007213:Indomethacin