Electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose composite gels and their visualization by fluorography.
We have developed techniques for the electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose gel slabs and subsequent fixation, impregnation, and fluorography of such electrophoretograms. The procedure permits the examination of newly synthesized proteoglycan subspecies using a rapid technique, previously unavailable for these labeled molecules.
D000293:Adolescent; D000328:Adult; D000818:Animals; D004285:Dogs; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005404:Fixatives; D006801:Humans; D008875:Middle Aged; D010779:Photofluorography; D011509:Proteoglycans / Q000032:analysis*; D013552:Swine
D005404:Fixatives; D011509:Proteoglycans