title: Effect of manipulation of phospholipid polar head group on low density lipoprotein metabolism in human cultured fibroblasts.
abstract: Modification of phospholipid polar head group was achieved by growing human cultured fibroblasts in medium devoid of serum and supplemented with N-methyl ethanolamine or N,N-dimethylethanolamine during 48 h. The corresponding phospholipids accounted for approximately 45% of total phospholipids. Whereas low density lipoprotein (LDL) binding was unaffected, LDL internalization and degradation appeared to be markedly reduced in the presence of N-methylethanolamine. N,N-dimethylethanolamine had no effect on the three studied parameters. These results emphasize the importance of phospholipid polar head group in LDL processing by receptor-mediated endocytosis.
mesh_terms: D002478:Cells, Cultured; D002794:Choline / Q000031:analogs & derivatives / Q000378:metabolism; D004705:Endocytosis; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008563:Membrane Lipids / Q000502:physiology*; D010743:Phospholipids / Q000502:physiology*; D011973:Receptors, LDL / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D008077:Lipoproteins, LDL; D008563:Membrane Lipids; D010743:Phospholipids; D011973:Receptors, LDL; D002794:Choline