title: Ultramicromethod for measuring the activity of terminal deoxynucleotidyl transferase.
abstract: We describe an ultramicromethod for measurement of terminal deoxynucleotidyl transferase activity. The materials used are as proposed by Hösli (Clin Chem 1977;23:1476-84) for antenatal diagnosis. The technique is based on the methods of Beutler and Kuhl (Am J Clin Pathol 1978;70:733-7) and Yasmineh et al. (Clin Chem 1980; 26:891-5). The procedure makes it possible to reduce the blood sample to 2 mL at the most and to reduce the cost of the measurement very considerably. The technique gives results similar to those given by the conventional techniques.
mesh_terms: D004253:DNA Nucleotidylexotransferase / Q000097:blood*; D004254:DNA Nucleotidyltransferases / Q000097:blood*; D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000097:blood / Q000201:enzymology; D008832:Microchemistry / Q000379:methods
chemical_list: D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase