title: Antibacterial activity of dilute povidone-iodine solutions used for ocular surface disinfection in dogs.
abstract: Bacterial cultures of specimens from healthy canine eyelids and ocular surfaces were found to demonstrate bacterial growth in 69.7% (53/76) of the eyes sampled. Organisms most commonly isolated included: Staphylococcus aureus, alpha-hemolytic Streptococcus sp, S epidermidis, and Escherichia coli. Evaluation of dilute povidone-iodine solutions for effectiveness as ocular surface disinfectants was conducted. Bacterial growth initially detected in 32 of 46 eyes was not detected after disinfection with a 2-minute scrub and 2-minute soaking procedure, using 1:2, 1:10, or 1:50 dilutions of a povidone-iodine solution that contained 1% available iodine. The eyelid and ocular surfaces of 16 eyes were disinfected with 1:100 povidone-iodine solution. Bacterial growth initially present in 10 of 16 eyes was present in 1 eye after disinfection and consisted of a single colony of E coli. After eyes were disinfected with 1:10, 1:50, or 1:100 povidone-iodine solutions, there was no evidence of corneal epithelial edema or sloughing. In 15 eyes subjected to disinfection with the 1:2 dilution, one instance of epithelial corneal edema was noticed. A 1:50 dilution of povidone-iodine is recommended as an ocular surface disinfectant for use in presurgical situations.
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects*; D004203:Disinfection; D004285:Dogs; D005123:Eye / Q000187:drug effects / Q000382:microbiology*; D005260:Female; D008297:Male; D009883:Ophthalmic Solutions; D011205:Povidone / Q000031:analogs & derivatives*; D011206:Povidone-Iodine / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D009883:Ophthalmic Solutions; D011206:Povidone-Iodine; D011205:Povidone