Two species of phospholipase C isolated from lymphocytes produce specific ratios of inositol phosphate products.
Chromatography of the soluble porcine lymphocyte phospholipase C on cellulose phosphate resolves the activity into two species. An HPLC method is described for separating the enzyme products, Ins 1,2 greater than P and Ins 1P. Use of these methods reveals that the two iso-enzymes liberate, with a high degree of reproducibility, characteristic ratios of the two products. This suggests that the amount of each product produced is an inherent property of the enzyme mechanism.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007295:Inositol Phosphates / Q000097:blood*; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology*; D013403:Sugar Phosphates / Q000097:blood*; D013552:Swine; D010738:Type C Phospholipases / Q000097:blood* / Q000302:isolation & purification
D007295:Inositol Phosphates; D007527:Isoenzymes; D013403:Sugar Phosphates; D010738:Type C Phospholipases