title: Radiation induced formation of giant cells in Saccharomyces uvarum. III: Effect of X-rays on nuclear division.
abstract: Spindle formation and nuclear division of budding and irradiated yeast cells (Saccharomyces uvarum) was investigated by fluorescence microscopy of protoplasted cells. Protoplasts were treated with antitubulin antibodies and DAPI, a fluorescent dye staining DNA. In budding yeast cells, duplication of spindle pole bodies as well as formation of complete 1-micron spindles and elongated 8-micron spindles were documented. In X-irradiated cells, spindle pole bodies were duplicated as well, forming the complete 1-micron spindle. Nuclei of giant cells have lost the elongation ability and remain in a "normal" G2-phase state, thus preventing nuclear as well as cellular division.
mesh_terms: D002453:Cell Cycle / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002467:Cell Nucleus / Q000528:radiation effects* / Q000648:ultrastructure; D002843:Chromatin / Q000528:radiation effects; D008856:Microscopy, Fluorescence; D012440:Saccharomyces / Q000166:cytology / Q000528:radiation effects*; D014965:X-Rays
chemical_list: D002843:Chromatin