Determination of AMP in the rat heart using skeletal muscle AMP deaminase.
A new simple enzymatic method for measuring AMP content in freeze-clamped rat heart is presented. The method is based on the ammonia estimation after the deamination of 5'-AMP by muscle 5'-adenylic acid deaminase. The minimum detectable amount of AMP was about 1.5 nmol. The recovery of AMP added to the tissue homogenate was 94%. The variance coefficient evaluated by assaying five samples from one tissue extract was equal to 5%. AMP content of rat heart (0.28 mumol/g wet tissue) is comparable with the values reported by others.
D000659:AMP Deaminase; D000249:Adenosine Monophosphate / Q000032:analysis*; D000641:Ammonia / Q000032:analysis; D000818:Animals; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000032:analysis*; D009709:Nucleotide Deaminases; D051381:Rats; D013379:Substrate Specificity
D000249:Adenosine Monophosphate; D000641:Ammonia; D009709:Nucleotide Deaminases; D000659:AMP Deaminase