title: Outer membrane protein antigens of Moraxella bovis.
abstract: Outer membranes were isolated from bovine isolates and type strains of Moraxella bovis, M phenylpyruvica, M lacunata, and M ovis by sodium N lauroyl sarcosinate extraction and differential centrifugation. Analysis of outer membranes from these organisms by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis revealed that all M bovis isolates shared a common polypeptide pattern that was readily distinguishable from other Moraxella spp. Nine major outer membrane protein bands were identified by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis analysis of M bovis. Immunoblotting of protein antigens of M bovis revealed several outer membrane proteins that seemed to be common antigens of all M bovis isolates.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009016:Moraxella / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins