[Carbohydrates in the liver microsome fraction of rats with hypovitaminosis A].
Accessibility for trypsin and sodium deoxycholate was determined in carbohydrates of glycoproteins in the liver microsome fraction of rats, which were kept for 80 days on retinol-deficient diet and received optimal amounts of vitamin A. It was found that the prevailing amount of hexose- and glucosamine-containing glycoproteins is located on the outer surface of membrane vesicles and only a smaller part of these proteins is submerged into the lipid layer of the membrane or is located on its inner surface. Above a half of protein bound with fucose and neuraminic acid is located in the lipid layer. Retinol deficiency leads to translocation of a portion of fucose- and hexose-containing proteins on the outer surface of vesicles and to a decrease of the share of these proteins in the hydrophobic membrane zone.
D000818:Animals; D050260:Carbohydrate Metabolism; D006023:Glycoproteins / Q000378:metabolism; D006868:Hydrolysis; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility; D014357:Trypsin; D014802:Vitamin A Deficiency / Q000378:metabolism*
D006023:Glycoproteins; D008565:Membrane Proteins; D014357:Trypsin
Uglevody vo fraktsii mikrosom pecheni krys s A-gipovitaminozom.