Detection of Clostridium difficile toxins A (enterotoxin) and B (cytotoxin) in clinical specimens. Evaluation of a latex agglutination test.
A new latex test, Culturette Brand Rapid Latex Test for detection of Clostridium difficile toxin A, was tested on 408 stool samples. In 247 frozen tissue culture supernate specimens previously obtained from patients with C. difficile-associated diarrhea (CAD), the latex test (enterotoxin) was positive in 182 (74%) as compared with 194 (79%) for the repeat tissue culture (P greater than 0.1) cytotoxin (toxin B) test. Testing of 161 fresh stool samples found the latex test superior to tissue culture (P less than 0.05) in cases of CAD (90% positivity vs. 70%), with the two tests being equal in both non-CAD diarrheal and non-diarrheal control groups. In vitro evaluation of 61 C. difficile isolates found all (100%) to be producers of enterotoxin A, while only 53 (87%) produced toxin B. The latex test for C. difficile toxin detection is a rapid, simple test for use in the diagnosis in CAD.
D001427:Bacterial Toxins / Q000032:analysis*; D003015:Clostridium Infections / Q000175:diagnosis*; D003603:Cytotoxins / Q000032:analysis*; D003967:Diarrhea / Q000209:etiology; D004768:Enterotoxins / Q000032:analysis*; D005243:Feces / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D007841:Latex Fixation Tests / Q000379:methods
D001427:Bacterial Toxins; D003603:Cytotoxins; D004768:Enterotoxins