A new hydrophobic anchor for the attachment of proteins to liposomal membranes.
The model enzyme alpha-chymotrypsin covalently modified by a phosphatidylethanolamine derivative has been attached to liposomal membranes in high yield. A maximal protein/lipid ratio of 5.4 X 10(-3) mol enzyme/mol lipid was achieved.
D002918:Chymotrypsin / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D008081:Liposomes; D017830:Octoxynol; D010714:Phosphatidylethanolamines / Q000138:chemical synthesis; D011092:Polyethylene Glycols / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D012997:Solvents
D008081:Liposomes; C049392:N-glutarylphosphatidylethanolamine; D010714:Phosphatidylethanolamines; D012997:Solvents; D011092:Polyethylene Glycols; D017830:Octoxynol; D002918:Chymotrypsin