Reversible inhibition of ovine ceruloplasmin by thiomolybdates.
The synthesis and purification of the sodium salts of di (MoO2S2(2-)), tri (MoOS3(2-)) and tetra (MoOS4(2-)) thiomolybdate is reported. All three compounds were reversible inhibitors of the ovine ceruloplasmin (Cp) catalysed oxidation of O-dianisidine. Na2MoO2S2 inhibited via a non-competitive mechanism whereas Na2MoOS3 showed mixed non competitive and Na2MoS4 competitive mechanisms. All showed upward curving slope replots. Molybdenum trisulfide (MoS3) was synthesized and displayed mixed type inhibition kinetics but with a linear slope replot. Preliminary evidence suggested that both MoOS3(2-) and MoS4(2-) may also be substrates for ovine Cp.
D000818:Animals; D002570:Ceruloplasmin / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D007700:Kinetics; D008982:Molybdenum / Q000494:pharmacology*; D012756:Sheep
C034539:dithiomolybdic acid; C034540:trithiomolybdic acid; D008982:Molybdenum; C020809:tetrathiomolybdate; D002570:Ceruloplasmin