Culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers.
In preliminary studies, a system for the propagation of respiratory syncytial virus (RSV) using cytodex 3 microcarriers was established. Optimal growth conditions were defined in culture to be a microcarrier concentration of 1.5 g/L with a cell inoculum density of 4 X 10(4) cells/ml tissue culture medium. Under these conditions growth coefficients were 3-fold greater in microcarriers when compared to conventional monolayer culture in roux culture flasks. Maximum yield of virus antigen was achieved after 8 days culture.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D007700:Kinetics; D009297:Nasal Mucosa / Q000166:cytology / Q000382:microbiology*; D011108:Polymers; D012136:Respiratory Syncytial Viruses / Q000254:growth & development*
D011108:Polymers