Whole-mount autoradiography study of DNA synthetic activity during postnatal development and androgen-induced regeneration in the mouse prostate.
Ventral and dorsolateral prostatic lobes (VP and DLP), obtained from mice at different ages and at different intervals after castration or treatment of castrated males with testosterone propionate (TP), were microdissected into two-dimensional arrays and incubated in vitro with 14C-thymidine. Labeled whole-mount specimens were fixed and dried onto glass slides, dipped into photographic emulsion, and processed autoradiographically. The morphological pattern of DNA synthetic activity was similar in the VP and DLP. During early postnatal periods (10-15 days after birth), DNA synthetic activity was highest at the distal ductal tips (near the capsule) and considerably lower in proximal ducts (near the urethra). At 30 days of age, DNA synthesis was almost totally confined to the distal ducts, with exceedingly low labeling in the proximal ductal areas. In the prostate of the intact or castrated adult, DNA synthesis was nearly absent throughout the gland, but silver grains were still observed on the ductal tips. During androgen-induced prostatic regeneration, DNA synthesis was detectable only in distal ducts 24 h after TP was administered. Labeling intensity reached a maximum on the third day of TP treatment in both distal and proximal ductal areas, thereafter, it subsided to focal labeling confined mostly to distal ducts. These results demonstrate that levels of DNA synthetic activity vary considerably within the prostate on a regional basis. Explanation of this heterogeneity in DNA synthetic activity within the prostate gland is fundamental to understanding the mechanism of androgenic regulation of prostatic growth and development.
D000375:Aging; D000818:Animals; D001345:Autoradiography; D004261:DNA Replication / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009919:Orchiectomy; D011467:Prostate / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013936:Thymidine