Bradykinin releases acetylcholine from myenteric plexus by a prostaglandin-mediated mechanism.
Release of [3H]acetylcholine (ACh) under the influence of bradykinin was measured from myenteric plexus-longitudinal muscle strips taken from guinea pig small intestine. Bradykinin stimulated the efflux of [3H]ACh in a dose-dependent manner. This stimulation by bradykinin was resistant to the effect of [Des-Arg9-Leu8]-bradykinin but not to indomethacin, indicating that the ACh-releasing action of bradykinin was mediated indirectly by a prostaglandin mechanism. Direct evidence for a stimulation of ACh release by prostaglandin E1 was obtained. Prostaglandin was able to stimulate ACh release in a dose-related fashion. The inhibition of bradykinin-induced ACh release by indomethacin was partly reversed by exogenous prostaglandin E1. These results suggest a neuromodulatory role for bradykinin in the enteric nervous system.
D000109:Acetylcholine / Q000378:metabolism*; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives / Q000494:pharmacology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007421:Intestine, Small / Q000294:innervation; D007700:Kinetics; D009197:Myenteric Plexus / Q000187:drug effects / Q000378:metabolism*; D011453:Prostaglandins / Q000502:physiology
D011453:Prostaglandins; D000527:Alprostadil; D000109:Acetylcholine; D001920:Bradykinin; D007213:Indomethacin