Optimum methanol concentration and solvent/peanut ratio for extraction of aflatoxin from raw peanuts by modified AOAC method II.
The amount of aflatoxin extracted from raw peanuts by using the water-slurry modification of AOAC Method II was determined for 49 different combinations of methanol concentrations and solvent/peanut ratio. Results indicate that the amount of aflatoxins B1 and B2 extracted from raw peanuts is a function of both methanol concentration and solvent/peanut ratio, and a cubic equation was developed, using regression techniques, to describe the combined effects. From the functional relationship, the predicted methanol concentration and solvent/peanut ratio that extracts the most aflatoxin B1 was computed to be 60.0% and 10.8 mL solvent/g peanuts, respectively. This combination extracted 12.1% more aflatoxin than did AOAC Method II.
D000348:Aflatoxins / Q000032:analysis*; D010367:Arachis / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D000432:Methanol; D012997:Solvents
D000348:Aflatoxins; D012997:Solvents; D000432:Methanol