title: Avian hepatic T-3 production by two pathways of 5'-monodeiodination: effects of fasting and patterns during development.
abstract: Two forms of iodothyronine 5'-monodeiodinase (5'-D) were studied in liver homogenates from adult and developing quail. The influence of fasting in adults and corticosterone treatment in embryonic quail on 5'-D also were examined. Liver homogenates were assayed for 5'-D activity in the presence of abundant substrate (T4) and cofactor (dithiothreitol; DTT). Generation of T3 during a 15 min incubation at 37 degrees C was assessed by an ethanol-based RIA. In adults, both Type I [the fraction of activity inhibited by propylthiouracil (PTU)] and a putative Type II (the PTU-insensitive fraction) were present in liver homogenates. Type II activity typically comprised about 30% of Total activity. Type I activity first appeared on day 15 of the 16.5 day incubation period, increased 20-fold to peak at hatching, then gradually declined to reach adult levels by 21 days of age. Type II activity was present at all developmental stages and was highest during the perinatal period. Corticosterone treatment in vivo on day 13 of development induced increases in both Type I and Type II activities in liver homogenates 24- and 48-h after treatment. This study demonstrates that in avian liver a putative Type II 5'-D activity (generally considered to be lacking in mammalian liver) is present and may be important in the maintenance of minimal concentrations of tissue T3 during fasting. Both types of 5'-D contribute to the developmental pattern of serum T3 concentrations. Type II comprises a large proportion of total activity during late embryonic life; Type I becomes predominant at the beginning of the perinatal period.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000375:Aging; D000818:Animals; D003345:Corticosterone / Q000494:pharmacology; D003370:Coturnix; D004625:Embryo, Nonmammalian; D004790:Enzyme Induction; D005215:Fasting; D005260:Female; D007453:Iodide Peroxidase / Q000096:biosynthesis / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D014284:Triiodothyronine / Q000096:biosynthesis*
chemical_list: D014284:Triiodothyronine; D007453:Iodide Peroxidase; D003345:Corticosterone