Glicentin 1-61 probably represents a major fraction of glucagon-related peptides in plasma of anaesthetized uraemic pigs.
Uraemia was induced in pigs by ligation of the renal vascular pedicle, and uraemic plasma was analysed for glucagon and glucagon-related peptides. A preponderance of large molecular weight (Mr) components comprising glicentin and moieties of slightly lower Mr was found, accounting for 73 +/- 3% (mean +/- SEM, n = 12) of the total plasma glucagon-like immunoreactivity. Comparisons with glicentin 1-61, produced by controlled, stepwise, consecutive digestion of purified natural glicentin with carboxypeptidases (carboxypeptidase A followed by carboxypeptidase B, and again by carboxypeptidase A and B), gel filtration, ion exchange chromatography, reverse phase HPLC and radioimmunoassays for the glucagon sequences 6-15 and 19-29 and for the glicentin sequence 12-30 all indicate that glicentin 1-61 constitutes approximately 57% of the large Mr glucagon-related peptides found in uraemia in pigs.
D000595:Amino Acid Sequence; D000818:Animals; D002845:Chromatography; D002850:Chromatography, Gel; D005934:Glucagon / Q000097:blood / Q000276:immunology*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000097:blood / Q000276:immunology; D052336:Proglucagon; D011498:Protein Precursors / Q000097:blood / Q000276:immunology*; D011863:Radioimmunoassay; D013552:Swine; D014511:Uremia / Q000097:blood*
D010446:Peptide Fragments; D011498:Protein Precursors; D052336:Proglucagon; D005934:Glucagon