Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis.
Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus. However, the matrix of this low percentage gel is relatively weak, and one can separate the two forms of apoB in a slab gel apparatus only if one utilizes a gradient polyacrylamide gel or a higher percentage polyacrylamide gel which results in a poorer separation of the protein bands. We have developed an agarose-acrylamide gel electrophoretic method to separate the two major apoB forms. The gel is a mixture of 0.5% agarose and 2% acrylamide. The agarose-acrylamide method is fast, has the advantage of being able to be used on an analytical or preparative scale in a vertical slab gel apparatus, and the gel is of sufficient strength to be used in immunoblotting and/or radioautography.
D020106:Acrylamide; D000178:Acrylamides; D001055:Apolipoproteins B / Q000097:blood*; D003101:Collodion; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D008079:Lipoproteins, VLDL / Q000097:blood; D010209:Paper; D012685:Sepharose
D000178:Acrylamides; D001055:Apolipoproteins B; D008079:Lipoproteins, VLDL; D020106:Acrylamide; D003101:Collodion; D012685:Sepharose