Staphylococcus aureus protein A--antibody-mediated haemagglutination for the analysis of cross-reactivity between phenol-water extracts of Bacteroides fragilis.
Passive haemagglutination (PHA) and Staphylococcus aureus protein A--antibody-mediated haemagglutination (SAPA-AMHA) were used to analyse the cross-reactions of rabbit antisera against four strains of Bacteroides fragilis. There was cross-reactivity between all the strains tested but strain-specific reactions were obtained with three strains. Two to 32-fold higher antibody titres were obtained with SAPA-AMHA than with PHA. The antigen concentration required in inhibition assays was up to 32-fold higher for PHA than for SAPA-AMHA. The latter is a simple and superior alternative to PHA for such studies.
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000276:immunology*; D001441:Bacteroides fragilis / Q000276:immunology*; D003429:Cross Reactions; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D019800:Phenol; D010636:Phenols; D013205:Staphylococcal Protein A / Q000276:immunology; D014867:Water
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D010636:Phenols; D013205:Staphylococcal Protein A; D014867:Water; D019800:Phenol