Morphology of endoplasmic reticulum and Golgi elements following microinjection of rat liver microsomes into Xenopus laevis oocyte cytoplasm.
Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuole, and identified by their association with specific morphological markers (peroxisomal cores associated with rough microsomes and lipoprotein particles with Golgi derivatives). Typical endoplasmic reticulum microsomes disappeared with time post-injection and seemed to be replaced by flattened cisternae of endoplasmic reticulum. Golgi fragments as defined by their content of lipoprotein particles became modified. Many were found associated with coated vesicles and some displayed membrane-coated regions. Furthermore lipoprotein particles were observed as integral components of Golgi stacks and were found within dilated rims in direct continuity with fenestrated Golgi saccules. The results suggest that the injected organelle fragments underwent transformation in vivo as a consequence of reconstitution.
D000818:Animals; D003593:Cytoplasm / Q000502:physiology / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D005260:Female; D006056:Golgi Apparatus / Q000032:analysis / Q000648:ultrastructure*; D008079:Lipoproteins, VLDL / Q000032:analysis; D008830:Microbodies / Q000648:ultrastructure; D008845:Microinjections; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000637:transplantation / Q000648:ultrastructure*; D009865:Oocytes / Q000648:ultrastructure*; D051381:Rats; D014982:Xenopus laevis
D008079:Lipoproteins, VLDL