title: Nucleotide sequence of the exclusion-determining locus of IncI plasmid R144.
abstract: The exclusion-determining locus (exc) of IncI plasmid R144 has been proposed to contain two overlapping genes. The nucleotide sequence of this locus, as presently determined, reveals an open reading frame with a coding capacity of 220 amino acids (aa) and with a promoter located upstream of the translation-initiation region. Consistent with the proposed overlapping gene arrangement, a second putative promoter was found within this coding region. Thus, a polypeptide identical to the 147 C-terminal aa of the larger polypeptide can be expressed from this second promoter. The 3'-noncoding region contains a sequence that is representative for a Rho-independent transcription terminator.
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics; D008970:Molecular Weight; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011487:Protein Conformation; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins