A digitonin-based procedure for the isolation of mitochondrial DNA from mammalian cells.
A procedure for the isolation of closed circular mitochondrial DNA (mtDNA) from cells permeabilized with digitonin is described. Compared to the standard procedure in which cells are broken after osmotic swelling, the digitonin-based procedure is more consistent and results in higher mtDNA yields.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D004072:Digitonin; D007739:L Cells; D051379:Mice; D009994:Osmolar Concentration
D004272:DNA, Mitochondrial; D004072:Digitonin