title: Immune serum to protein molecular weight standards for calibrating Western blots.
abstract: A method for calibrating proteins by Western blot analysis is described. Rabbit immune serum, specific for seven protein molecular weight standards, and goat anti-rabbit horseradish peroxidase conjugate are used to visualize standards after electrotransfer onto nitrocellulose. This method can be used as a reagent and procedure control.
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002138:Calibration; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D007120:Immunochemistry; D007875:Legionella; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D012015:Reference Standards
chemical_list: D001426:Bacterial Proteins; D007106:Immune Sera; D011506:Proteins; D003101:Collodion