title: Plasmid curing and generation of mutations induced with ethidium bromide in streptomycetes.
abstract: The DNA-intercalating agent ethidium bromide was used to eliminate plasmid DNA from streptomycetes. Other mutational events associated with chromosome changes occurred at high frequency; they resulted in phenotypic changes such as loss of enzyme activity, antibiotic resistance or ability to sporulate.
mesh_terms: D003114:Colony-Forming Units Assay; D004352:Drug Resistance, Microbial; D004996:Ethidium / Q000494:pharmacology*; D009154:Mutation; D009355:Neomycin; D010957:Plasmids / Q000187:drug effects*; D013171:Spores, Bacterial / Q000187:drug effects; D013302:Streptomyces / Q000187:drug effects* / Q000502:physiology
chemical_list: D004996:Ethidium; D009355:Neomycin