title: Peroxidase catalyzed aggregation of plasmid pBR322 DNA by benzidine metabolites in vitro.
abstract: The interaction of plasmid DNA and metabolites of benzidine produced by the action of horseradish peroxidase and hydrogen peroxide was investigated by a combination of agarose gel electrophoresis and autofluorography. Benzidine becomes irreversibly bound to the DNA to form a macromolecular structure that can no longer penetrate a 0.8% agarose gel. Other carcinogens such as o-dianisidine, o-tolidine and amino-fluorene also reacted in this way but N4-tetramethylbenzidine and the non-carcinogenic 3,5,3',5',tetramethylbenzidine did not.
mesh_terms: D001560:Benzidines / Q000378:metabolism* / Q000633:toxicity; D001711:Biotransformation; D002250:Carbon Radioisotopes; D004247:DNA / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000494:pharmacology*; D010544:Peroxidases / Q000494:pharmacology*; D010957:Plasmids
chemical_list: D001560:Benzidines; D002250:Carbon Radioisotopes; D004247:DNA; D006735:Horseradish Peroxidase; D010544:Peroxidases