[Role of the plasma membrane in the distribution of potassium, sodium and calcium in ram sperm during deep freezing].
Studied was the distribution of potassium, sodium, and calcium in ram semen stored at various temperatures: 25 degrees C, 4 degrees C, and -196 degrees C. In fresh semen the concentration of K in spermatozoa and seminal plasma was 259.5 +/- 14.5 mg% and 56.4 +/- 2.1 mg%, respectively; of Na it was 38.6 +/- 3.1 mg% and 175.9 +/- 7.3 mg%; and of Ca it was 56.2 +/- 4.3 mg% and 28.0 +/- 1.9 mg%. It was found that the content of K and Ca in the spermatozoa was higher than in the plasma, while with Na the concentration in the plasma was higher than in the spermatozoa. Cooling and deep freezing of ram semen led to the redistribution of ions between spermatozoa and its natural medium, consisting in the increase in the intracellular concentration of Na and in the decrease in that of K and Ca, whereas the extracellular concentration of Na decreased and that of K and Ca increased. At all temperature values tested the concentration of K in the sperm cell was 260, 194, and 141 mg%, and that in the medium was 20.7, 21.4, and 25.6 mg%; with Na these values were 35.4, 42.3, and 49.1 mg% and 65.3, 59.1, and 45.9 mg%, and with Ca -48.0, 26.8, and 24.7 mg%, and 9.3, 9.8, and 10.8 mg%, respectively. Deep freezing was found to impair ion transport; it made it impossible for the sperm cell to restore the ion equilibrium due to irreversible changes in the structure and function of the plasmal membrane of spermatozoa.
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005615:Freezing; D008297:Male; D011188:Potassium / Q000378:metabolism*; D012662:Semen Preservation; D012756:Sheep / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D013696:Temperature
D012964:Sodium; D011188:Potassium; D002118:Calcium
Roliata na plazmenata membrana v razpredelenieto na kaliĭ, natriĭ i kaltsiĭ v spermata na koch pri dŭlboko zamraziavane.