Inhibition of glutathione S-transferase P type-positive foci development by linolic acid hydroperoxides and their secondary oxidative products in a rat in vivo mid-term test for liver carcinogens.
The effects of linolic acid hydroperoxides (product A) and secondary oxidative products of product A (product B), were examined in an in vivo mid-term test for hepatocarcinogens or hepatopromoters in rats. The number of placental type of glutathione S-transferase (GST-P)-positive foci of the liver was significantly reduced in rats given diethylnitrosamine (DEN) followed by products A (4.64 +/- 1.09, P less than 0.05) or B (3.62 +/- 1.65, P less than 0.01) as compared to the controls given carcinogen alone (6.31 +/- 2.82). The area of GST-P positive foci was also significantly reduced in rats given DEN followed by product B (0.30 +/- 0.21, P less than 0.05) as compared to the controls (0.47 +/- 0.23). These results suggest that linolic acid hydroperoxides or their secondary oxidative products are not hepatocarcinogens and rather may possess inhibitory potential for liver carcinogenesis.
D000818:Animals; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D004052:Diethylnitrosamine; D005982:Glutathione Transferase / Q000032:analysis*; D008041:Linoleic Acids / Q000378:metabolism / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000517:prevention & control; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011916:Rats, Inbred F344
D008041:Linoleic Acids; D008054:Lipid Peroxides; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; C008285:linoleic acid hydroperoxide; D004052:Diethylnitrosamine; D005982:Glutathione Transferase