The origins of the sciatic nerve and changes in neuropeptides after axotomy: a double labelling study using retrograde transport of true blue and vasoactive intestinal polypeptide immunohistochemistry.
One month after sciatic nerve section, only dorsal root ganglion cells which take up True blue applied to the cut end of the nerve show vasoactive intestinal polypeptide immunoreactivity. This indicates that VIP expression is only in cells with damaged axons. Motor axons with True blue-positive perikarya in the ventral horn originate from the fourth to sixth lumbar segments whereas unmyelinated and small myelinated sensory nerves terminate in the third to fifth lumbar segments of the spinal cord.
D000818:Animals; D001369:Axons / Q000502:physiology*; D001572:Benzofurans / Q000378:metabolism*; D001692:Biological Transport; D003714:Denervation; D006651:Histocytochemistry; D007120:Immunochemistry; D008297:Male; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D012584:Sciatic Nerve / Q000033:anatomy & histology* / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D001572:Benzofurans; D009419:Nerve Tissue Proteins; D014660:Vasoactive Intestinal Peptide; C038675:true blue