title: In vivo voltammetric measurement of extracellular DOPAC levels in the anteromedial prefrontal cortex of the rat.
abstract: Differential pulse voltammetric recordings with carbon fiber electrodes performed in vivo in the anteromedial prefrontal cortex of the rat yielded 3 oxidation peaks at -100, +100 and +300 mV, respectively. Pharmacological manipulations revealed that 3,4-dihydroxyphenylacetic acid (DOPAC) is the main contributor to the oxidation current recorded at +100 mV (peak 2). Thus, systemic administration of FLA 63 did not alter whereas pargyline caused a disappearance of cortical peak 2. Moreover, haloperidol and sulpiride increased peak 2 amplitude both in normal and in N-(2-chloroethyl)N-ethyl-2-bromobenzylamine (DSP4)-lesioned rats. Peak 2 was detected only in those prefrontal cortex regions known to receive a dopamine input. It is concluded that in vivo voltammetry with carbon fiber electrodes is a useful means of monitoring dopaminergic activity in the prefrontal cortex of the rat.
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D005406:Bis(4-Methyl-1-Homopiperazinylthiocarbonyl)disulfide / Q000494:pharmacology; D004563:Electrochemistry; D005625:Frontal Lobe / Q000032:analysis* / Q000378:metabolism; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D010648:Phenylacetates / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013469:Sulpiride / Q000494:pharmacology
chemical_list: D001596:Benzylamines; D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D005406:Bis(4-Methyl-1-Homopiperazinylthiocarbonyl)disulfide; D013469:Sulpiride; D006220:Haloperidol; C012102:DSP 4