Modulation of protein biosynthesis during early stages of differentiation in retinoic acid treated F9 teratocarcinoma cells.
Modulation of protein biosynthesis by retinoic acid during induction of differentiation of F9 teratocarcinoma stem cells was investigated by using computerized analysis of double label autoradiography of two-dimensional polyacrylamide gels. As early as 6 h after induction increased synthesis of 5 and decreased synthesis of 2 proteins occur. By 12 h after induction, synthesis of 13 proteins is elevated and by 24 h that of 17. At 24 h the range of stimulation is from two- to fourfold, as demonstrated by a 3H:14C ratio divided by the mode ratio. Examination of the Gaussian distributions of frequency of ratio indicates that many subtle changes in protein synthesis accompany the development of the new phenotype.
D000818:Animals; D001345:Autoradiography / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D013724:Teratoma / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology
D009363:Neoplasm Proteins; D014212:Tretinoin