Kinetic study of the reaction between trinitrobenzenesulfonic acid and amino acids with a trinitrobenzenesulfonate ion-selective electrode.
Potentiometric studies of the reaction between trinitrobenzenesulfonic acid (TNBS) and several amino acids with the TNBS electrode indicate that the reaction is first-order with respect to TNBS and amino acid concentration. The reaction is zero-order with respect to hydroxide concentration at pH greater than 10.5, indicating that the nonprotonated amino group is the reactive species. Rate constants were calculated for each amino acid and a simple mechanism of the reaction is proposed.
D000409:Alanine; D000596:Amino Acids; D004566:Electrodes; D005998:Glycine; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides; D007700:Kinetics; D008956:Models, Chemical; D009578:Nitrobenzenes / Q000032:analysis*; D011199:Potentiometry; D014302:Trinitrobenzenesulfonic Acid / Q000032:analysis*
D000596:Amino Acids; D006878:Hydroxides; D009578:Nitrobenzenes; D014302:Trinitrobenzenesulfonic Acid; D000409:Alanine; D005998:Glycine