Binding of cardiotoxin analogue III from Formosan cobra venom to FL cells.
The binding equilibrium at 37 or 0 degrees C of 125I-cardiotoxin analogue III (CT III) to fetal lung (FL) cells (cultured human amnion cells) was achieved within 1 h, and the binding at 37 degrees C was irreversible. The Scatchard analysis at 37 degrees C on the binding of 125I-CT III indicated that FL cells had two types of binding sites with different association constants. The association constant and the number of high-affinity sites was 1.1 X 10(10) mol-1 or 2.8 X 10(6) per FL cell, respectively. At 37 or 0 degrees C, the cytotoxicity of CT III paralleled the amount of bound CT III to FL cells, and at 37 degrees C was inhibited by the presence of acidic phospholipids.
D001665:Binding Sites; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D004179:Cobra Cardiotoxin Proteins / Q000378:metabolism* / Q000633:toxicity; D004546:Elapid Venoms / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D014343:Trypan Blue
D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms; D014343:Trypan Blue