Gas chromatographic-mass spectrometric analysis of volatile constituents in saliva.
Present methods for the development of metabolic profiles are limited to the use of headspace techniques and solvent extraction methods. A new method for the development of saliva profiles which provides information complementary to existing analyses has been developed. The results of the developed methodology provide a reliable, reproducible method for metabolic profiling. Gas chromatographic-mass spectrometric analysis of the volatile constituents provided positive identification of 39 compounds. Application of the developed protocol toward the investigation of saliva as a vehicle for the non-invasive detection of certain pathological states, specifically diabetes mellitus and liver disorders, may be possible.
D002784:Cholesterol / Q000378:metabolism; D003920:Diabetes Mellitus / Q000175:diagnosis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008107:Liver Diseases / Q000175:diagnosis; D008297:Male; D010061:Ovulation Detection / Q000379:methods; D012463:Saliva / Q000032:analysis*; D012737:Sex Factors
D007202:Indicators and Reagents; D002784:Cholesterol