A radioenzymatic method for the determination of tissue 10-formyltetrahydrofolate.
A radioenzymatic method for the determination of tissue 10-formyltetrahydrofolate is described based on the stable ternary complex formed from methylenetetrahydrofolate, tritiated fluorodeoxyuridylate and thymidylate synthase. Tissue extract 10-formyltetrahydrofolate is deacylated with 10-formyltetrahydrofolate deacylase and the tetrahydrofolate formed is converted to methylenetetrahydrofolate using formaldehyde. Mouse tissue 10-formyltetrahydrofolate levels and their stability to extraction procedures are described.
D000818:Animals; D005575:Formyltetrahydrofolates / Q000032:analysis*; D008099:Liver / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D013763:Tetrahydrofolates / Q000032:analysis*; D014316:Tritium
D005575:Formyltetrahydrofolates; D013763:Tetrahydrofolates; D014316:Tritium