Rapid cation-exchange high-performance liquid chromatographic procedure for the separation and quantitation of hemoglobins S, C, and O Arab in cord blood samples.
A rapid method for separating and quantitating hemoglobin (Hb) variants in cord blood samples using cation high-performance liquid chromatography (HPLC) is described. The procedure is a modification of a previously published method, and uses a weak cation-exchange Brownlee-3CM column and Bis-Tris-KCN-Na acetate developers. A chromatogram can be completed in 10 minutes. The slow-moving variants, Hbs S, C, and O Arab, can be completely separated from each other and are identified by their elution times relative to Hb A. Hb E elutes as a shoulder on the descending side of Hb A, which is characteristic for this variant in this procedure. Differentiation between heterozygous, homozygous, and Hb X-beta+-thalassemia conditions is easily made.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006444:Hemoglobin C / Q000302:isolation & purification; D006451:Hemoglobin, Sickle / Q000302:isolation & purification; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D011190:Potassium Cyanide; D013789:Thalassemia / Q000175:diagnosis
D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; D006444:Hemoglobin C; C032031:hemoglobin Arab; D011190:Potassium Cyanide