Inhibition by indomethacin of naloxone-precipitated opiate withdrawal contraction in isolated guinea pig myenteric plexus-longitudinal muscle preparation.
The isolated myenteric plexus-longitudinal muscle preparation from guinea pigs which had previously been administered morphine chronically 100 mg/kg/day, 7 days) shows a pronounced contraction when exposed to naloxone. Exposure of the preparation to indomethacin (6.7 micrograms/ml) for 10 minutes prior to naloxone inhibits this withdrawal response. A single dose of indomethacin, 1 mg/kg, given on the 6th day on morphine, also inhibits the naloxone contraction. These results suggest the involvement of a prostaglandin in the opiate withdrawal response in the ileum.
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D006168:Guinea Pigs; D007082:Ileum / Q000503:physiopathology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D009021:Morphine Dependence; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000503:physiopathology; D009197:Myenteric Plexus / Q000503:physiopathology; D009270:Naloxone / Q000037:antagonists & inhibitors*; D013375:Substance Withdrawal Syndrome / Q000503:physiopathology*
D009270:Naloxone; D000109:Acetylcholine; D007213:Indomethacin