Flow microcalorimetric study of immobilized enzyme kinetics using the co-immobilized glucose oxidase-catalase system.
The kinetics of the bi-substrate enzyme glucose oxidase (EC 1.1.3.4) (with catalase, EC 1.11.1.6) co-immobilized on glass was studied using flow microcalorimetry. Oxygen and glucose external-internal diffusion effects were resolved.
D001234:Aspergillus niger / Q000201:enzymology; D002151:Calorimetry; D002374:Catalase / Q000378:metabolism*; D004058:Diffusion; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005898:Glass; D005949:Glucose Oxidase / Q000378:metabolism*; D007700:Kinetics; D010100:Oxygen
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D002374:Catalase; D010100:Oxygen