Development and characterization of pantothenic acid transport in brain.
In vitro, the transport of [3H]pantothenic acid into and from rabbit brain slices was studied. In newborn rabbits and throughout development, forebrain and cerebellar slices were able to accumulate and phosphorylate [3H]pantothenic acid comparably to slices from adults. The accumulation and phosphorylation of [3H]pantothenic acid by adult forebrain slices were not decreased by substitution of LiCl for NaCl in the artificial CSF or by addition of short-chain fuels (e.g., 5 mM pyruvate or acetoacetate) to the medium. However, probenecid and ouabain (both 1 mM) and medium-chain fatty acids (e.g., 0.1 mM octanoate, nonanoate, and decanoate) profoundly inhibited [3H]pantothenic acid accumulation by forebrain slices but not intracellular phosphorylation and conversion to [3H]CoA. There in vitro results suggest that brain slices accumulate pantothenic acid by a saturable system (probably facilitated diffusion) that is sensitive to inhibition by probenecid and medium-chain fatty acids.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002831:Choroid Plexus / Q000378:metabolism; D004027:Diencephalon / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology; D007700:Kinetics; D010042:Ouabain / Q000494:pharmacology; D010205:Pantothenic Acid / Q000378:metabolism*; D010766:Phosphorylation; D011339:Probenecid / Q000494:pharmacology; D011817:Rabbits; D013687:Telencephalon / Q000378:metabolism
D005227:Fatty Acids; D010205:Pantothenic Acid; D010042:Ouabain; D011339:Probenecid