The teratogenic potential and dose-response of dermally administered ethylene glycol monomethyl ether (EGME) estimated in rats with the Chernoff-Kavlock assay.
The Chernoff and Kavlock in vivo assay was used to study the teratogenic potential of ethylene glycol monomethyl ether (EGME). It was administered to pregnant rats on days 6-17 (inclusive) of gestation by the dermal route at an application rate of 10 ml kg-1 body weight of 3%, 10%, 30% and 100% EGME with physiological saline as the solvent. 100% was lethal to the pregnant rats, 30% was lethal to the fetuses, 10% produced small litter sizes with poor survival and 3% had no adverse effects. By the 'rules' developed to evaluate results from the assay, 10% was judged to be a potentially teratogenic dose in the rats. The study also demonstrated the usefulness of this assay for pre-screening or additional investigations for suspected teratogens when route of exposure could be important in determining the potential hazard.
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000287:Administration, Topical; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005026:Ethylene Glycols / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D008722:Methods; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin
D005026:Ethylene Glycols; C005219:methyl cellosolve