The existence of an envelope on extracellular cowpox virus and its antigenic relationship to the vaccinia envelope.
Virus released from cowpox infected cells was demonstrated by electron-microscopy to be surrounded by an envelope not present on mature intracellular virus. Enveloped cowpox had an isopycnic density of 1.23 g/ml, was infectious and neutralized by antibodies specific for the envelope antigens of vaccinia virus but not neutralized by antibodies specific for intracellular naked vaccinia virus. Only 1 per cent of the total intracellular virus yield was released as enveloped virus. The major cowpox glycoprotein (76 K) and a 44 K glycoprotein did not comigrate with any vaccinia glycoproteins whereas cowpox glycoproteins at 42 K and 20-23 K did coelectrophores with vaccinia glycoproteins. All of the mentioned cowpox glycoproteins were precipitated by vaccinia antiserum.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002499:Centrifugation, Density Gradient; D005110:Extracellular Space; D006023:Glycoproteins / Q000276:immunology; D009500:Neutralization Tests; D011817:Rabbits; D014616:Vaccinia virus / Q000254:growth & development / Q000276:immunology / Q000648:ultrastructure*; D014764:Viral Proteins / Q000276:immunology; D014771:Virion / Q000648:ultrastructure
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins