title: Correlative ultrastructural and electrophysiological studies of sperm-egg interactions of the sea urchin, Lytechinus variegatus.
abstract: The sequence of ultrastructural events following the onset of the sperm-induced conductance increase in eggs of the sea urchin, Lytechinus variegatus, was investigated. Eggs voltage clamped at -20 mV were fixed 1 to 20 sec after onset of the conductance increase caused by single sperm. Continuity between the plasma membranes of the sperm and egg was first detected 5 sec after onset of the conductance increase. The earliest stages of formation of the fertilization cone coincided with the establishment of continuity of the gamete plasma membranes. At 6 to 8 sec after the initial conductance increase cortical granule dehiscence was first observed in the immediate vicinity where continuity of the gamete plasma membranes had occurred. These observations are consistent with the conclusion that opening of ion channels at fertilization precedes fusion of the sperm and egg plasma membranes, while exocytosis of cortical granules is initiated following fusion of the sperm and egg plasma membranes.
mesh_terms: D000177:Acrosome / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D003594:Cytoplasmic Granules / Q000502:physiology; D005089:Exocytosis; D005260:Female; D005306:Fertilization; D008297:Male; D008561:Membrane Fusion; D008564:Membrane Potentials; D008854:Microscopy, Electron; D010063:Ovum / Q000502:physiology* / Q000648:ultrastructure; D012617:Sea Urchins / Q000196:embryology / Q000502:physiology*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure; D013997:Time Factors