Physalaemin-like immunoreactive peptides from rabbit stomach.
Two physalaemin (PHY)-like immunoreactive peptides, designated PHLIPs, have been purified from extracts of rabbit stomach tissue. Fast atom bombardment/mass spectrometry (FAB/MS) indicated that the m/z values for the PHLIP protonated molecular ions were 867.419 and 796.4. FAB/tandem MS spectra, coupled with a knowledge of the amino acid composition and the aid of a computerized fragment-matching program, indicated the amino acid sequences to be: (formula; see text) The sequences of PHLIPs-7 and -8 were confirmed with synthetic peptides. The PHY-antiserum cross-reactivity of the PHLIPs reflects homology at amino acid residues 1, 3, 4 and 5 for the mammalian and amphibian residues.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D007705:Kinins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000138:chemical synthesis; D010803:Physalaemin / Q000032:analysis* / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013270:Stomach / Q000032:analysis*
D000596:Amino Acids; D007705:Kinins; D010446:Peptide Fragments; D010455:Peptides; D010803:Physalaemin