Proliferative characteristics of progeny derived from normal human hemopoietic progenitor cells.
Cell cycle times of cells derived from human hemopoietic progenitor cells were measured directly by using 5-bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. Umbilical cord cells were cultured in the mixed-colony assay for 96 h prior to the addition of BrdU and then harvested at 6-12 h intervals. Individual cell cycle times ranging from 8.5-23.4 h were observed while population mean cell cycle times ranged from 17.5-24 h. These results show that some early progeny of hemopoietic progenitor cells have short cell cycle times and that there is considerable heterogeneity within the proliferating cell population.
D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle; D002455:Cell Division; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007700:Kinetics
D001973:Bromodeoxyuridine