[Neutrophil agglutination test in sensitized guinea pigs].
Guinea pigs sensitized with DNCB, K2Cr2O7, epidian 5, hardener Z1 and dye B, in which epidermal tests confirmed sensitization, exhibited, 24 hours after the test termination, increased agglutination of neutrophils. In the blood of animals sensitized with dye B using the Polak and Türk, as well as Magnusson and Kligman techniques, percentage indices of agglutination, whether spontaneous or induced by adding 25 micrograms of the allergen, were 2,40 and 8,90 for DNCB, 6,72 and 13.30 for K2Cr2O7, 7.61 and 12.59 for epidian 5, 8.69 and 11.63 for hardener Z1, 1.38 and 2.15 and 2.17 and 3.21 for dye B. The average (from 6 control groups) number of neutrophils, both those self-agglutinated and those induced by allergen, was 2.54 and 3.24, respectively. No increase in neutrophils agglutination in guinea pigs sensitized with the Alekseeva and Sumska technique-exhibiting no allergy--was found. The results point to the usability of determinations of neutrophils agglutination in blood as an auxiliary index confirming sensitivity to a given allergen.
D000818:Animals; D004137:Dinitrochlorobenzene; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000209:etiology; D004853:Epoxy Resins; D005260:Female; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D008086:Lissamine Green Dyes; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D011192:Potassium Dichromate; D014266:Trientine
D004137:Dinitrochlorobenzene; D004853:Epoxy Resins; D008086:Lissamine Green Dyes; C024537:green 5; C009752:epidian; D014266:Trientine; D011192:Potassium Dichromate
Test zlepności granulocytów objetnochłonnych we krwi uczulonych świnek morskich.