Production of Chinese hamster monoclonal antibody to a human cell-surface antigen using a hamster-human somatic cell hybrid as antigen.
Hybridomas producing monoclonal antibody (MAb) of predefined specificity were isolated from a Chinese hamster that had received injections of a Chinese hamster-human somatic cell hybrid. A standard method for the production of murine hybridomas was used to produce Chinese spleen lymphocyte X murine plasmacytoma hybridomas, and they were screened for complement-mediated cytotoxicity against a panel of Chinese hamster-human somatic cell hybrids and agglutination of human erythrocytes. Two hybridomas were established in tissue culture following limiting dilution cloning, and their reactivity with a panel of Chinese hamster-human somatic cell hybrids indicates that they are specific for the previously identified human cell-surface antigen a1. The Chinese hamster appears to respond preferentially to human antigens of Chinese hamster-human somatic cell hybrids, and it will serve as a useful tool for the production of MAb specific for human cell-surface antigens expressed in the many well-characterized Chinese hamster-human somatic cell hybrids available.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology*; D006224:Cricetinae / Q000276:immunology*; D003412:Cricetulus / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface