title: Amino acid sequence of vitamin D-dependent calcium-binding protein from bovine cerebellum.
abstract: The amino acid sequence of vitamin D-dependent calcium-binding protein from bovine cerebellum has been determined. It is composed of 260 amino acid residues and its N-terminus is acetylated. The molecular mass is calculated to be 29 851 Da. The presence of six calcium-binding sites (I-VI) has been proposed, two of them (sites II and VI) have lost their calcium-binding function through amino acid replacements, and the other four are able to bind calcium. Six calcium-binding domains are supposed to be derived from two gene duplications of the two ancestral calcium-binding domains. In comparison with the sequence of chick intestinal calcium-binding protein deduced from a cDNA sequence [(1985) Nucleic Acids Res. 13, 8867-8881], the bovine calcium-binding protein is two amino acid residues shorter at the N-terminus and the other parts show 78.5% identity.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002531:Cerebellum / Q000032:analysis*; D002645:Chickens; D004247:DNA; D007422:Intestines / Q000032:analysis; D008970:Molecular Weight; D010446:Peptide Fragments; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D010446:Peptide Fragments; D064030:S100 Calcium Binding Protein G; D004247:DNA; D002118:Calcium