title: Skin calcium-binding protein is a parvalbumin of the panniculus carnosus.
abstract: Skin calcium-binding protein (SCaBP) is a calcium binding protein purified from whole rat skin. It has a molecular weight of approximately 12,000 daltons but migrates at Mr 13,000 on sodium dodecyl sulfate (SDS)-polyacrylamide gels. On nitrocellulose blots of SDS-polyacrylamide gels, 6 different antisera to SCaBP reacted equally well with SCaBP and parvalbumin (PV), an 11,500-dalton calcium-binding protein purified from rat skeletal muscle, which also migrates at Mr 13,000 on SDS-polyacrylamide gels. Rabbit antiserum to muscle PV also recognized both PV and SCaBP, and either protein absorbed specific antibodies against either antigen from both types of antisera. Soluble protein extracts from whole adult rat and mouse skin contained a Mr 13,000 protein which was recognized on nitrocellulose blots of SDS gels by both antisera. Blots of extracts from epidermis, dermis, whole skin, and skin scraped on the dermal side to remove hypodermal tissue revealed that the Mr 13,000 PV/SCaBP cross-reacting antigen was restricted to the hypodermal tissue removed by scraping. Immunofluorescent staining of Bouin-fixed skin sections with these antisera confirmed the localization of PV/SCaBP to the panniculus carnosus, a hypodermal muscle layer. Newborn mouse skin does not contain this antigen. Additional polypeptides of Mr 10,500 and 12,000 on SDS gels of extracts from the epidermis of newborn and adult rats and mice were found to be immunoreactive with anti-SCaBP serum. These polypeptides were not recognized by the PV antiserum, and the reactivity of anti-SCaBP for these antigens was not absorbed by purified PV or SCaBP. Our results indicate that SCaBP is antigenically indistinguishable from PV and is localized in the adult rodent panniculus carnosus, and that antisera to SCaBP are polyspecific, recognizing epidermal proteins in addition to SCaBP/PV.
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002478:Cells, Cultured; D003429:Cross Reactions; D006651:Histocytochemistry; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D010320:Parvalbumins / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D051381:Rats; D012867:Skin / Q000032:analysis*
chemical_list: D002135:Calcium-Binding Proteins; D007106:Immune Sera; D009124:Muscle Proteins; D010320:Parvalbumins