Studies on the biosynthesis of ecdysone by the Y-organs of Carcinus maenas.
High specific activity tritiated ecdysone precursor, 2,22,25-trideoxyecdysone, was incubated with Y-organs from intermoult and premoult shore crabs. Several metabolites were identified among which ecdysone and 25-deoxyecdysone. The concomitant production of these 2 molecules by Y-organs and their subsequent hydroxylation at C-20 by peripheral tissues, provide an explanation for the presence of both 20-hydroxyecdysone and ponasterone A (25-deoxy-20-hydroxyecdysone) in the circulating haemolymph of crabs.
D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D002783:Cholestenones; D002851:Chromatography, High Pressure Liquid; D004440:Ecdysone / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000378:metabolism; D004441:Ecdysterone / Q000031:analogs & derivatives / Q000378:metabolism; D004702:Endocrine Glands / Q000378:metabolism; D007700:Kinetics
D002783:Cholestenones; D004440:Ecdysone; C007424:3 beta,14 alpha-dihydroxy-5 beta-cholest-7-en-6-one; D004441:Ecdysterone; C008999:ponasterone A