Identification of novel N-glucuronides in rat bile after administration of 450191-S, a 1H-1,2,4-triazolyl benzophenone derivative.
5-[2-Aminoacetamido)methyl]-1-[p-chloro-2-(o-chlorobenzoyl)phenyl] -N,N-dimethyl-1H-s-triazole-3-carboxamide hydrochloride dihydrate (450191-S) is a ring-opened derivative of 1,4-benzodiazepine, which is activated by desglycylation and subsequent cyclization. After 450191-S administration, rat bile contained three novel conjugates which released active metabolites possessing the 1,4-benzodiazepine structure through beta-glucuronidase hydrolysis. Since the released metabolites have no functional groups to conjugate with glucuronic acid, we speculated that the aglycone might be the ring-opened form of 1,4-benzodiazepine which spontaneously cyclizes after the release of glucuronic acid. This possibility was tested by chemically reducing the ketone group of the ring-opened 1,4-benzodiazepine glucuronate conjugates, which would prevent the spontaneous ring closure reaction after the release of the glucuronic acid moiety. The NaBH4 reduction of the ketone of the benzophenone moiety of the conjugates and subsequent treatment with beta-glucuronidase allowed identification of the reduced aglycones with authentic samples using gas chromatography-mass spectrometry.
D000818:Animals; D001646:Bile / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000378:metabolism*; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D008297:Male; D051381:Rats; D014230:Triazoles / Q000378:metabolism* / Q000494:pharmacology
D005965:Glucuronates; D014230:Triazoles; C041075:rilmazafone; D005966:Glucuronidase