title: Effect of Pasteurella multocida and Haemophilus pleuropneumoniae toxins on swine alveolar macrophages.
abstract: Supernatants were obtained from 18 hr. broth cultures of Pasteurella multocida strains D82 and D62 (serotype D, toxigenic), Kobe 6 (type D, non-toxigenic), A50 and X73 (type A, non-toxigenic), Haemophilus pleuropneumoniae serotypes 1, 5 and 6, Haemophilus sp. taxon "minor group" (2 strains) and an avirulent serotype 1 strain of H. pleuropneumoniae. The supernates were filtered, pH-neutralized and tested for cytotoxicity after incubation for 18 hours in the presence of swine alveolar macrophage monolayers. Supernatants from H. pleuropneumoniae serotypes 1, 5 and 6 were cytocidal.
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004731:Endotoxins / Q000494:pharmacology; D006168:Guinea Pigs; D006190:Haemophilus / Q000276:immunology*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D010325:Pasteurella / Q000276:immunology*; D013552:Swine
chemical_list: D001427:Bacterial Toxins; D004731:Endotoxins