The interaction of rifamycin-SV with the hepatic transport and sulfation of taurolithocolic acid in rats.
Sulfated and non sulfated lithocholic acid were analyzed in serum, bile and urine of bile-fistula Sprague-Dawley female rats by gas-liquid chromatography and thin-layer chromatography. In rats (group A) bile was collected during a 3 h saline infusion followed by a 3 h intravenous infusion of taurolithocholate; animals of group B and C were infused with both taurolithocholate and rifamycin-SV, but in the animals of group C the renal pedicles were ligated. Serum bile acids showed a significant increase only in animals of group C, during the infusion of rifamycin-SV. In bile of animals of group A sulfated lithocholic acid represented 18% of total, but was not found in bile of animals of group B and C, while a significant increase of sulfated lithocholic acid was evident in urine of animals of group B. The results indicate that rifamycin-SV probably interferes with the hepatic secretion of sulfated lithocholic acid in the rat.
D000818:Animals; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D005260:Female; D008095:Lithocholic Acid / Q000031:analogs & derivatives*; D008099:Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012294:Rifamycins / Q000494:pharmacology*; D013431:Sulfates / Q000378:metabolism*; D013658:Taurolithocholic Acid / Q000378:metabolism*
D001647:Bile Acids and Salts; D012294:Rifamycins; D013431:Sulfates; D013658:Taurolithocholic Acid; D008095:Lithocholic Acid