Uptake and blood pressure studies in the chick embryo following treatment with a teratogenic dose of dopamine.
We report on the ventricular pressor response and regional (head, heart, and body) uptake of dopamine hydrochloride (DA) following topical application of a teratogenic dose to the stage 24 chick embryo. Embryos treated with DA exhibited a significant increase in mean ventricular blood pressure (MVBP) at 0.5 and 1 hour after treatment when compared to saline-treated control embryos. The time of the elevated MVBP correlated with the time of the peak uptake of DA in the embryonic heart. Even though the level of DA in the heart remained high for 9 hours, there was no measurable increase in blood pressure beyond 1.5 hours, suggesting unresponsiveness to DA or perhaps conversion of DA to its metabolites. These experiments have correlated a physiologic response of a known cardiovascular teratogen to its uptake in the embryo.
D000818:Animals; D001692:Biological Transport; D001794:Blood Pressure / Q000187:drug effects*; D002642:Chick Embryo; D004298:Dopamine / Q000378:metabolism / Q000633:toxicity*; D006321:Heart / Q000187:drug effects / Q000196:embryology*; D006352:Heart Ventricles / Q000196:embryology; D007700:Kinetics; D013723:Teratogens
D013723:Teratogens; D004298:Dopamine