Regulation of glycerol 3-phosphate oxidation in mitochondria by changes in membrane microviscosity.
The inhibition of glycerol 3-phosphate oxidation by oleic acid correlates with changes in membrane microviscosity monitored by the steady-state fluorescence anisotropy of DPH. The dynamic measurements indicate that the changes of both the limiting anisotropy and rotational relaxation time occur in a concentration range where the enzyme activity is strongly inhibited.
D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D006224:Cricetinae; D004161:Diphenylhexatriene; D005454:Fluorescence Polarization; D005994:Glycerophosphates / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008051:Lipid Bilayers / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010084:Oxidation-Reduction; D014783:Viscosity
D005994:Glycerophosphates; D008051:Lipid Bilayers; D009829:Oleic Acids; D004161:Diphenylhexatriene; D019301:Oleic Acid; C029620:alpha-glycerophosphoric acid