Covalent coupling of neuralizing factors from Xenopus to Sepharose beads: no decrease of inducing activity.
Two neural inducing factors extracted from Xenopus gastrulae, a basic protein from ribonucleoprotein particles and an acidic protein from the high speed supernatant were covalently bound to CNBr-Sepharose or cross-linked CNBr-Sepharose particles. The protein-Sepharose complexes cannot be taken up by the competent ectoderm cells, but both factors remain fully active. The inducing activity is not due to a release of the bound factors. The experiments suggest that both neural inducing factors act on the cell surface of the competent ectoderm cells.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D003488:Cyanogen Bromide; D004475:Ectoderm / Q000187:drug effects; D005775:Gastrula / Q000032:analysis; D009420:Nervous System / Q000187:drug effects / Q000196:embryology*; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology; D012261:Ribonucleoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D012685:Sepharose; D014982:Xenopus laevis
D011506:Proteins; D012261:Ribonucleoproteins; D012685:Sepharose; D003488:Cyanogen Bromide