Effect of glucocorticoid on glycosaminoglycans synthesis in cultured mouse embryonic palatal mesenchymal cells.
The effect of glucocorticoids (GC) on the synthesis of glycosaminoglycans (GAGs) in mesenchymal cells obtained from palatal shelves of mouse embryos was studied. Both hyaluronic acid (HA) synthesis and sulfated GAG synthesis were inhibited with triamcinolone acetonide (TA) in a concentration-dependent manner. However, the degree of inhibition of synthesis with TA was greater for HA than for the sulfated GAGs. To determine whether the inhibitory effects of TA on HA and sulfated GAGs synthesis were mediated through GC receptors, we performed experiments using progesterone, vitamin B6, and molybdate. The inhibitory effect of TA was antagonized by all three. The results obtained in the present study suggest that the inhibition of synthesis of HA and sulfated GAGs with GC is mediated through GC receptors and is involved in cleft palate induction by GC.
D000818:Animals; D002478:Cells, Cultured; D003238:Connective Tissue / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D004622:Embryo, Mammalian; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D007700:Kinetics; D008648:Mesoderm / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008982:Molybdenum / Q000494:pharmacology; D010159:Palate / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011736:Pyridoxine / Q000494:pharmacology; D014222:Triamcinolone Acetonide / Q000494:pharmacology*
D006025:Glycosaminoglycans; C044659:molybdate; D011374:Progesterone; D008982:Molybdenum; D014222:Triamcinolone Acetonide; D011736:Pyridoxine