[Determination of the standard oxyhemoglobin dissociation curve in horses. Effects of temperature, pH and diphosphoglycerate].
The equine blood oxyhemoglobin dissociation curve has been traced in its entirety in standard conditions and the effects of temperature, pH and 2,3-diphosphoglycerate on this curve have been measured. When compared to that of human blood, the curve showed a higher oxygen affinity of hemoglobin (23.8 +/- 0.8 versus 26.6 mm Hg). The effect of the pH, expressed by d log P50/dpH, was found to be identical in man and horse (-0.47). The effect of temperature, however, expressed by d log P50/dT, proved to be lower in the horse (0.016 versus 0.024). The P50 showed an increase of 1 mm Hg each time 2,3-diphosphoglycerate was experiencing an elevation of 4 mumol/gHb in the horse. The results obtained from this experiment are intended for the replacement of the values related to the human blood which have been found to be somewhat inadequate for the horse blood, when the data of blood gases are given in algorithms.
D019794:2,3-Diphosphoglycerate; D000818:Animals; D004163:Diphosphoglyceric Acids / Q000097:blood*; D005260:Female; D006736:Horses / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D010100:Oxygen / Q000097:blood; D010108:Oxyhemoglobins / Q000032:analysis*; D012015:Reference Standards; D012044:Regression Analysis; D012737:Sex Factors; D013696:Temperature
D004163:Diphosphoglyceric Acids; D010108:Oxyhemoglobins; D019794:2,3-Diphosphoglycerate; D010100:Oxygen
Détermination de la courbe de dissociation standard de l'oxyhémoglobine du cheval et influence, sur cette courbe, de la température, du pH et du diphosphoglycérate.