Subcellular distribution of selenium in the liver from rats fed selenium from fish: selenocystine and inorganic selenite.
Four groups of rats of a normal selenium status were given different selenium compounds during a long-term feeding experiment (28 days). The selenium supplementations (per kg diet) were sodium selenite (1 mg), selenocystine (2 mg), and two different concentration levels of selenium from fish (0.1 and 1 mg). Differential pelleting of liver homogenates demonstrated that selenium was present in all the subcellular fractions, with a recovery of 55-60% in the cytosols. Gel permeation high-performance liquid chromatography of the cytosol fractions demonstrated the presence of protein-bound selenium at a molecular weight of 70,000 daltons. The subcellular distributions as well as the protein binding of selenium in the cytosols were identical in all dietary groups. This indicates a similar long-term liver metabolism of the four selenium compounds tested in the rat.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003553:Cystine / Q000031:analogs & derivatives / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004032:Diet; D005399:Fishes; D008099:Liver / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D016566:Organoselenium Compounds; D011485:Protein Binding; D051381:Rats; D020887:Selenious Acid; D012643:Selenium / Q000008:administration & dosage / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism*
D016566:Organoselenium Compounds; C009226:selenocystine; D003553:Cystine; D020887:Selenious Acid; D012643:Selenium