Two-dimensional gel electrophoretic analysis of lectin receptors in the bovine interphotoreceptor matrix.
Proteins and glycoproteins of the bovine interphotoreceptor matrix (IPM) with or without neuraminidase treatment was analysed by two-dimensional gel electrophoresis combined with Western blotting and staining with seven horseradish peroxidase-labeled lectins. More than 80 spots of proteins and glycoproteins were revealed on the gel. Nineteen spots (or groups of spots) were revealed by staining with five lectins [concanavalin A, wheat germ agglutinin (WGA), peanut agglutinin (PNA), Ricinus communis agglutinin-1 (RCA-1) and soybean agglutinin (SBA)]; some of those spots were specific for one lectin and others reacted with several lectins. We could not detect distinct spots reacting with Dolichos biflorus agglutinin or Ulex europaeus agglutinin-1. Neuraminidase digestions of the IPM increased and unmasked the binding spots for PNA, RCA-1 and SBA. The spots of WGA-receptors without neuraminidase treatment were mostly identical to the receptors for PNA, RCA-1 and SBA, which became prominent after the digestion. Spots reacting with RCA-1 were mostly identical to the spots of SBA-receptors. The spots reacting with PNA coincided only partially with the spots reacting with RCA-1 and SBA.
D000818:Animals; D002417:Cattle; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins; D008970:Molecular Weight; D009439:Neuraminidase; D010786:Photoreceptor Cells / Q000032:analysis*; D037121:Plant Lectins; D011952:Receptors, Concanavalin A / Q000032:analysis; D011975:Receptors, Mitogen / Q000032:analysis*; D013025:Glycine max
D037102:Lectins; D037121:Plant Lectins; D011952:Receptors, Concanavalin A; D011975:Receptors, Mitogen; C032650:Ricinus communis lectin receptor; C038223:peanut agglutinin receptor; C027503:wheat germ agglutinin receptor; D003101:Collodion; D009439:Neuraminidase