Study of chromatin structure in ataxia-telangiectasia cells.
Micrococcal nuclease was used as a probe to study chromatin structure in control and ataxia-telangiectasia cells. The rate and extent of release of acid-soluble nucleotide was similar in both cell types. Production of mono- and oligonucleosomes by micrococcal nuclease as determined by gel electrophoresis also failed to reveal differences in chromatin structure between control and ataxia-telangiectasia cells. Radiation exposure did not significantly alter the kinetics of digestion. These results indicate that there are no gross alterations in chromatin structure in ataxia-telangiectasia cells.
D001260:Ataxia Telangiectasia / Q000235:genetics*; D002467:Cell Nucleus / Q000528:radiation effects; D002843:Chromatin / Q000648:ultrastructure*; D006801:Humans; D007700:Kinetics; D008836:Micrococcal Nuclease / Q000378:metabolism; D009707:Nucleosomes / Q000648:ultrastructure*
D002843:Chromatin; D009707:Nucleosomes; D008836:Micrococcal Nuclease