Growth stimulating activity on 3T3 fibroblasts of the molecular weight 6,500-peptide purified from rat pancreatic juice.
Growth stimulating activity of the molecular weight 6,500-peptide purified from rat pancreatic juice was measured on 3T3 fibroblasts. This peptide was reported to be a cholecystokinin-releasing peptide and to stimulate pancreatic enzyme secretion in the rat small intestine in response to food intake. Incorporation of [3H]thymidine and [35S]methionine into 3T3 was significantly stimulated and the cell number was also increased after 24-48 hr incubation with 10-100 ng/ml of the peptide. The increase in [3H]thymidine incorporation was dose-related and started 12 hr after the incubation, a peak being reached 24 hr after the incubation. These results show that this peptide exhibits growth stimulating activity to the mammalian cells, and suggest that the peptide might have a physiological effect in vivo.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D010189:Pancreatic Juice / Q000032:analysis*; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D006133:Growth Substances; D011506:Proteins; D013936:Thymidine