Modified clearing method to show sieve tubes in minor veins of leaves.
Leaf samples of Glycine max and numerous other dicotyledonous species were cleared by a common, well established procedure modified by using more concentrated (10% w/v) aqueous NaOH, and by leaving samples in NaOH for 2-4 weeks and in chloral hydrate for 3 days, all at room temperature. A single dye, chlorazol black E (1 g/100 ml absolute ethanol), is used to stain for 3-6 min. Samples are mounted with the lower epidermis upward. Sieve tubes in favorable material can be seen in minor veins and vein endings.
D006651:Histocytochemistry / Q000379:methods; D059828:Plant Cells