Effect of dietary 7-ketocholesterol, pure, or oxidized cholesterol on hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity, energy balance, egg cholesterol concentration, and 14C-acetate incorporation into yolk lipids of laying hens.
Experiments were conducted to study the effect of 7-ketocholesterol (7-k) in the presence or absence of pure cholesterol (PCH) or oxidized cholesterol (OCH) in diets of laying hens on reproductive performance and several parameters of cholesterol metabolism. In the first experiment, cholesterol synthesis and transport was examined by the in ovo incorporation of 14C-acetate into yolk triglycerides and cholesterol. Energy balances were also conducted. In the second experiment, hepatic 3 hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity was measured in vitro to evaluate potential cholesterol synthesis. In both experiments, reproductive performance and egg yolk cholesterol concentration were measured. Dietary PCH or OCH (.5%) significantly reduced relative acetate incorporation into yolk cholesterol, while 7-k (.025%) had no effect on carbon flow from acetate into egg cholesterol. While 7-k alone did not alter total yolk cholesterol concentration, it moderated the effect of PCH or OCH on increasing yolk cholesterol concentration. No consistent effects of dietary sterols on reproductive performance or energy balance were observed. Hepatic HMG CoA reductase activity was dramatically suppressed by feeding PCH or OCH and moderately suppressed by 7-k. In combination with PCH or OCH, 7-k did not further depress enzyme activity. The observations that 7-k alone depressed hepatic HMG-CoA reductase activity, without changing relative acetate incorporation into yolk cholesterol while limiting cholesterol deposition in egg yolk from PCH or OCH, is interpreted to mean that 7-k may stimulate sterol transport and excretion while limiting cholesterol synthesis.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D004530:Egg Yolk; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D007653:Ketocholesterols / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D010063:Ovum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism
D000085:Acetates; D007653:Ketocholesterols; D008055:Lipids; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases; C003001:7-ketocholesterol