Selective labelling of Cys-10 on actin.
Conditions are described for the selective modification of Cys-10 on actin achieved following the blocking of the more reactive Cys-374. Labelling of Cys-10 did not affect the formation of actin filaments. This residue should be capable of serving as a site for fluorescence donors or acceptors and thus will be a useful locus to probe F-actin structure.
D000199:Actins / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D003545:Cysteine; D005033:Ethylmaleimide; D005452:Fluoresceins; D009682:Magnetic Resonance Spectroscopy; D009132:Muscles; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013917:Thrombin; D014783:Viscosity
D000199:Actins; D005452:Fluoresceins; D010446:Peptide Fragments; C015296:5-iodoacetamidofluorescein; D013917:Thrombin; D003545:Cysteine; D005033:Ethylmaleimide