Changes in protein turnover in rat uterus during pregnancy.
The adaptive growth and protein turnover of the rat uterus were studied during the 21 days of gestation and up to 3 days after parturition. Despite large increases (13-fold) in uterine size during gestation, the fractional rate of protein synthesis (measured in vivo) remained unchanged when compared with nonpregnant tissue values of 44 +/- 5%/day. However, decreases were found in the rate of protein breakdown after implantation (i.e., 75% on day 7 and 28% on day 11) and in the activity of cathepsin D (i.e., 33 and 85% on days 8 and 16 of gestation). Changes in the degradative processes would therefore appear to be primarily responsible for the massive uterine growth during pregnancy. In contrast to the uterus the fractional rates of synthesis in the placenta and fetus progressively decreased during gestation. After parturition the uterus rapidly returned to its normal size by a combination of cellular atrophy and cell loss. After 2 days, a complementary decrease in the fractional rate of synthesis (30%) and an increase in protein degradation (2-fold) explained the process of involution.
D000818:Animals; D004247:DNA / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism / Q000502:physiology; D006128:Growth; D007743:Labor, Obstetric; D051379:Mice; D008815:Mice, Inbred Strains; D010920:Placenta / Q000378:metabolism / Q000502:physiology; D049590:Postpartum Period; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*; D051381:Rats; D014599:Uterus / Q000254:growth & development / Q000378:metabolism*
D011257:Pregnancy Proteins; D004247:DNA