Protein degradation in bupivacaine-treated muscles. The role of extracellular calcium.
The time course of protein degradation and the influence of extracellular calcium and the calcium-channel blocker verapamil were investigated by measuring tyrosine release from the isolated rat soleus muscle exposed to the local anaesthetic agent bupivacaine. Degradation rates were reduced during the first 60-90 min and subsequently increased in muscles treated with bupivacaine concentrations of 1.5 mM or higher. When calcium was omitted from the incubation medium the initial reduction in degradation was greater and the subsequent increase was reduced or prevented. Overall, verapamil (10(-5)M or 10(-6)M) did not significantly alter the degree or time-course of protein degradation.
D000818:Animals; D002045:Bupivacaine / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology*; D005260:Female; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011920:Rats, Inbred WF; D014443:Tyrosine / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
D009124:Muscle Proteins; D014443:Tyrosine; D014700:Verapamil; D002118:Calcium; D002045:Bupivacaine