title: Use of a copper-phthalocyanine membrane electrode for rapid preliminary detection of polycyclic mutagens.
abstract: Preliminary screening of polycyclic mutagens is achieved within 20 min by using a biomimetic electrode composed of an oxygen electrode and a copper-phthalocyanine membrane. When benzo[alpha]pyrene (0.05 mM) was added to the buffer solution in the presence of 0.98 M hydrogen peroxide, the current of the phthalocyanine electrode decreased. A linear relationship was obtained between the current decrease and the benzo[alpha]pyrene concentration over the range 0.19-0.60 mM. The minimum measurable concentration for benzo[alpha]pyrene was 0.01 mM. Such responses were not obtained for other organic compounds such as alcohol, ether, n-hexane and cyclohexane. The copper-phthalocyanine membrane electrode has selectively detected polycyclic mutagens such as amino acid pyrolysis products. The current decrease was 1.18-1.46 microA when 0.05 mM amino acid pyrolysis products were employed.
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis; D003300:Copper; D004566:Electrodes; D007211:Indoles; D054833:Isoindoles; D009153:Mutagens / Q000032:analysis*; D011083:Polycyclic Compounds / Q000032:analysis*
chemical_list: D007211:Indoles; D054833:Isoindoles; D009153:Mutagens; D011083:Polycyclic Compounds; D001564:Benzo(a)pyrene; D003300:Copper; C013647:phthalocyanine