title: Hydroxylation and glucuronidation of various xenobiotics by hepatic microsomes from the fetal lamb, pregnant ewe and human fetus.
abstract: The ability of microsomes isolated from liver of pregnant ewes and their fetuses at near term to catalyze the biotransformation of benzo[a]pyrene, hexobarbital, meperidine, methadone and morphine was investigated. Cytochromes P-450 and b5, NADPH and NADH cytochrome c reductase, methadone and meperidine N-demethylase and morphine glucuronyltransferase activities were detected in microsomes from both maternal and fetal livers. Fetal hepatic microsomes however, lacked the ability to catalyze the hydroxylation of hexobarbital and benzo[a]pyrene.
mesh_terms: D000818:Animals; D001711:Biotransformation; D003640:Dealkylation; D005260:Female; D005333:Fetus / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D006801:Humans; D006900:Hydroxylation; D066298:In Vitro Techniques; D008099:Liver / Q000196:embryology; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012756:Sheep
chemical_list: D005965:Glucuronates; D004364:Pharmaceutical Preparations; D006899:Mixed Function Oxygenases