[Limits of detection of nuclear DNA aneuploidy by FCM].
The limits of FCM detection of nuclear DNA aneuploidy were determined by simulation of the relationship between the coefficient of variation (CV) and DNA difference of two populations using a microcomputer. Under ideal conditions, the minimal DNA difference necessary for a bimodal peak is almost twice the CV. When the DNA difference is below the value, a single peak with a large CV and/or a shoulder is formed. Cases with a larger CV than expected in a single population should be practically treated as a population mixed with the near-diploid line.
D000782:Aneuploidy; D003201:Computers; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D008838:Microcomputers; D008957:Models, Genetic; D009369:Neoplasms / Q000235:genetics
D004273:DNA, Neoplasm