title: Double label autoradiography--an improvement.
abstract: A convenient method for double-label autoradiography is described that uses an aqueous mountant, Gelutol (polyvinyl alcohol), which keeps the gelatin spacer in the final autoradiograph permanently swollen to a thickness of around 18 microns in contrast to its 5 microns thickness during exposure of the autoradiograph. This greatly improves optical discrimination between upper and lower layers without the loss of sensitivity or resolution that would result if a 18 microns spacer were used during exposure.
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002250:Carbon Radioisotopes; D002453:Cell Cycle; D005780:Gelatin; D051379:Mice; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D014316:Tritium; D005780:Gelatin