title: Early increase in phosphatidyl choline synthesis by choline and transmethylation pathways in spreading fibroblasts.
abstract: Phosphatidyl choline (PC) synthesis in trypsinized and reattaching fibroblasts during the spreading state was studied by incorporation of [14C]choline and [methyl-14C]methionine. The choline and phosphatidyl-ethanolamine (PE) transmethylation pathways were both transiently increased about 2-fold during the first 2 h after replating. Maximum increase appeared to be simultaneous with maximum spreading. Incorporation of [32P]orthophosphate showed that the increase in PC synthesis was specific and most probably related to establishment of cell-substrate adhesion sites.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D002794:Choline / Q000378:metabolism*; D006224:Cricetinae; D005347:Fibroblasts; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D008745:Methylation; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000096:biosynthesis
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D008715:Methionine; C038344:methionine methyl ester; D002794:Choline