High-performance liquid chromatographic method for the determination of etoposide in plasma using electrochemical detection.
A quantitative analytical method has been established for the determination of a semi-synthetic epipodophyllotoxin, etoposide, in plasma. The method employs reversed-phase high-performance liquid chromatography and electrochemical detection. Sample preparation consisted of extraction with 1,2-dichloroethane followed by phase separation, evaporation of the organic phase, and reconstitution of the residue. Observed recoveries were 76.8 and 87.5% for 50 and 500 ng/ml, respectively. The method had a linear range of 10-1000 ng/ml. Correlation coefficients of 0.997 or greater were obtained during validation experiments and study sample analysis.
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004563:Electrochemistry; D005047:Etoposide / Q000097:blood*; D006801:Humans; D007700:Kinetics; D011786:Quality Control
D005047:Etoposide