An enzyme-linked immunosorbent assay for 6-keto PGF1 alpha.
An enzyme-linked immunosorbent assay for 6-keto prostaglandin F1 alpha, a stable metabolite of prostacyclin, has been developed. The assay allows quantitation of 6-keto PGF1 alpha in the range 1-200 pg/0.1 ml and shows very low cross reactivity to nine other prostaglandins. Dose dependent stimulation by thrombin of 6-keto PGF1 alpha formation in human endothelial cells in culture has been used to verify the assay. Quantitation by the enzyme linked immunosorbent assay agrees closely with determination by radioimmunoassay.
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D002478:Cells, Cultured; D003429:Cross Reactions; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011863:Radioimmunoassay; D013917:Thrombin / Q000494:pharmacology
D015121:6-Ketoprostaglandin F1 alpha; D013917:Thrombin