Nucleotide sequence of a cDNA clone encoding Scylliorhinus caniculus protamine Z2.
A cDNA library was constructed from a protamine-enriched fraction of dogfish (Scylliorhinus caniculus) mRNA. The nucleotide sequence of a 440-bp insert was determined, and its produced protein sequence confirmed its identification as a cysteine-rich protamine Z2 [Martinage, A., Gusse, M., Belaiche, D., Sautiere, P. and Chevaillier, P. (1985) Biochim. Biophys. Acta 831, 172-178]. The frequency of utilization of the different triplets coding for arginine, which represents 30-70% of the total amino acid residues for trout, mouse and dogfish protamines, is discussed. An alternative repetitive sequence of CGC-AGG was found in the N terminus of the protein. Analysis of the 3' flanking region after the mRNA-terminating TAA codon identified an inverted repeat sequence and an ACCA sequence, which may be possible vestiges of a histone-like termination signal.
D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D003001:Cloning, Molecular; D004247:DNA; D004284:Dogfish / Q000235:genetics*; D005815:Genetic Code; D005822:Genetic Vectors; D008297:Male; D009693:Nucleic Acid Hybridization; D011479:Protamines / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid; D012754:Sharks / Q000235:genetics*
D011479:Protamines; D012333:RNA, Messenger; C050556:protamine Z2; D004247:DNA