Automatic reading of DNA sequencing gel autoradiographs using a large format digital scanner.
We have developed a large format digital scanner for several applications in nucleic acid analysis. Here we describe the scanning of autoradiographs of DNA sequencing gels and a set of programs for reading the base sequence. The programs correct distortions in the gel, recognize bands by their characteristic shape and assign bases to bands by weighting band position and intensity. The sequence read in this way is as accurate as that read by an expert.
D001345:Autoradiography / Q000295:instrumentation; D001483:Base Sequence; D003201:Computers; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D008433:Mathematics; D012984:Software
D004247:DNA