title: Cyclization of phenylacetyl-L-cysteinyl-D-valine to benzylpenicillin using cell-free extracts of Streptomyces clavuligerus.
abstract: Benzylpenicillin, a typical antibiotic produced by some species of fungi, was obtained by direct cyclization of the heteropeptide phenylacetyl-L-cysteinyl-D-valine using cell-free extracts of Streptomyces clavuligerus. This is the first description of evidence of the synthesis of benzylpenicillin from a non natural molecule using a bacterial enzyme.
mesh_terms: D002474:Cell-Free System; D003500:Cyclization; D004151:Dipeptides / Q000378:metabolism*; D004798:Enzymes / Q000494:pharmacology; D010088:Oxidoreductases; D010400:Penicillin G / Q000096:biosynthesis*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D004151:Dipeptides; D004798:Enzymes; C049989:phenylacetylcysteinylvaline; D010088:Oxidoreductases; C038370:isopenicillin N synthetase; D010400:Penicillin G