A versatile low-cost apparatus for cell electrofusion and other electrophysiological treatments.
Instrumentation is described which will generate AC fields and DC square pulses for cell electrofusion, electroporation and other electrophysiological experiments. The equipment can generate a constant or intermittent AC field and a single square pulse, either alone or in combination. It can also generate a train of intermittent square pulses. The components for the instrumentation are commercially available and can be assembled by persons with little or no training in electronics. A design is given for a mass treatment chamber that can be easily constructed and viewed with a microscope.
D002468:Cell Physiological Phenomena; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D018521:Plant Physiological Phenomena; D011523:Protoplasts / Q000502:physiology