Incorporation of adenosine and adenine into hypoxanthine nucleotides of fresh red blood cells.
Incorporation of adenosine and adenine into hypoxanthine nucleotides of fresh red blood cells was monitored using 8-14C-adenosine and 8-14C-adenine added to the incubation medium containing adenosine, pyruvate and inorganic phosphate (APP medium). Using 8-14C-adenosine it was shown that 21.7% of the isotope contained in the incubation medium penetrated red blood cells. Of that quantity about 50% becomes incorporated into nucleotides. Of the isotope 5.3% was found in hypoxanthine nucleotides (1.3% in ITP and 4.0% in IMP). During incubation of red blood cells in APP medium fortified with the 8-14C-adenine about 95% of isotope penetrated into cells and 60% of that quantity became incorporated into nucleotides. In hypoxanthine nucleotides only trace amounts of isotope were found (0.12% in IMP and 0.13% in ITP).
D000225:Adenine / Q000097:blood*; D000241:Adenosine / Q000097:blood*; D002250:Carbon Radioisotopes; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007291:Inosine Monophosphate / Q000096:biosynthesis / Q000097:blood*; D007292:Inosine Nucleotides / Q000097:blood*; D007293:Inosine Triphosphate / Q000096:biosynthesis / Q000097:blood*
D002250:Carbon Radioisotopes; D007292:Inosine Nucleotides; D007291:Inosine Monophosphate; D007293:Inosine Triphosphate; D000225:Adenine; D000241:Adenosine