Inhibition of pneumococcal autolysis in lysis-centrifugation blood culture.
The recovery of Streptococcus pneumoniae from the Isolator lysis-centrifugation blood culture has been low in many studies. The poor survival of pneumococci was not due to toxicity of the Isolator medium but to autolysis before plating. This autolysis was completely inhibited by adding 10 mM phosphorylcholine to the Isolator medium.
D001433:Bacteriolysis / Q000187:drug effects*; D001769:Blood / Q000382:microbiology*; D002794:Choline / Q000031:analogs & derivatives*; D003470:Culture Media; D006801:Humans; D010767:Phosphorylcholine / Q000494:pharmacology*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000302:isolation & purification*
D003470:Culture Media; D010767:Phosphorylcholine; D002794:Choline