title: Chromosomal map location of the alpha-hemolysin structural gene in Staphylococcus aureus NCTC 8325.
abstract: The alpha-hemolysin structural gene, hly+, previously cloned, insertionally inactivated, and introduced into the chromosome by allele replacement (M.O. O'Reilly, J.C.S. De Azavedo, S. Kennedy, and T.J. Foster, Microb. Pathog. 1:125-138, 1986), was shown by protoplast fusion and transformation to be in the gene order purC-hly-uraB-omega[chr::Tn916]1101-thrB on the chromosome of Staphylococcus aureus NCTC 8325. This location is clearly distinct from that of the agr determinant, a regulatory gene affecting several extracellular proteins, including alpha-hemolysin, located between tmn and ilv.
mesh_terms: D000483:Alleles; D001427:Bacterial Toxins / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000502:physiology*; D003001:Cloning, Molecular; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D006460:Hemolysin Proteins / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin