Detection of an unstable murine hemoglobin.
3H-leucine was used in vitro to label newly synthesized adult alpha and beta globins in reticulocytes removed from normal (Hba-b/Hba-b;Hbb-s2/Hbb-s2 and alpha-thalassemic (Hba-b2(th)/Hba-b;Hbb-s2/Hbb-s2) mice. The ratio of synthesis of beta-s2major: beta-sminor globins was 71:29 in reticulocytes from normal mice and 55:45 in reticulocytes from alpha-thalassemic mice. The two beta-globins are structurally identical except for a Val----Glu substitution at position 60. Denaturation of these mouse hemoglobins in isopropanol indicated that the tetramer containing the beta-s2major globin is unstable.
D000433:1-Propanol / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D005914:Globins / Q000032:analysis; D006441:Hemoglobin A / Q000032:analysis*; D007930:Leucine / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011489:Protein Denaturation; D012156:Reticulocytes / Q000032:analysis; D013789:Thalassemia / Q000097:blood
D046911:Macromolecular Substances; D005914:Globins; D006441:Hemoglobin A; D000433:1-Propanol; D007930:Leucine