The distribution of polypeptide YY-like immunoreactivity in rat tissues.
The distribution of peptide YY (PYY)-like immunoreactivity (IR) in rat tissues was determined by specific RIA after extraction with boiled 1 N acetic acid. The high concentration of PYY-IR was observed in the gastrointestinal tract, with concentrations gradually increasing from the duodenum to the end of colon. The concentration of PYY-IR in the colon was 298.7-449.5 pmol/g tissue (approximately 100-200 times more than that in the duodenum). Pituitary and pancreas contained measurable amounts of PYY-IR (6.8 and 6.3 pmol/g tissue). The concentration of PYY-IR in the mucosa was higher than that in the muscular layer in the small intestine, cecum, colon, and rectum. The ratio of the mucosal PYY-IR to the muscular PYY-IR was highest in the distal small intestine (4.7-6.8). Sephadex G-50 gel chromatography of the colon extracts revealed the one PYY-IR peak which corresponds to [125I]PYY. The gradual increase of PYY-IR from the duodenum to the end of the colon is different from the distribution of other known gut peptides.
D000818:Animals; D001923:Brain Chemistry; D002432:Cecum / Q000032:analysis; D003106:Colon / Q000032:analysis; D007413:Intestinal Mucosa / Q000032:analysis; D007421:Intestine, Small / Q000032:analysis; D008297:Male; D010179:Pancreas / Q000032:analysis; D019894:Peptide YY; D010455:Peptides / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis; D011706:Pyloric Antrum / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012007:Rectum / Q000032:analysis; D014018:Tissue Distribution
D010455:Peptides; D019894:Peptide YY