Hydrophobicity modulation as a tool for the purification of histidyl peptides.
A methodology is described for purification of histidyl peptides based on the changes in hydrophobicity induced by the specific and reversible modification of histidine residues by ethoxyformic anhydride. The mixture of modified peptides is subjected to HPLC; peptides containing modified histidines are detected at 242 nm, recovered, deethoxyformylated, and rechromatographed under the same conditions, then being detected at 220 nm. This procedure allows their isolation free from contaminants.
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004047:Diethyl Pyrocarbonate; D006639:Histidine; D010446:Peptide Fragments / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin; D014867:Water
D010446:Peptide Fragments; D014867:Water; D006639:Histidine; D014357:Trypsin; D004047:Diethyl Pyrocarbonate