Effect of amino acids on luminol-dependent chemiluminescence generated by peripheral blood polymorphonuclear leukocytes.
Individual amino acids and amino acid mixtures caused a dose-dependent increase in chemiluminescence generated by peripheral blood polymorphonuclear leukocytes. A visual assay for opsonophagocytosis, however, failed to identify any quantitative differences between leukocytes incubated with amino acids and those incubated in amino-acid-free solutions. The results of this study suggest that the presence of amino acids may interfere with the proper interpretation of luminol-dependent chemiluminescence curves.
D000596:Amino Acids / Q000494:pharmacology*; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*
D000596:Amino Acids; D008165:Luminol