Characterization of three-fraction mycobacillin synthetase.
Mycobacillin synthetase lacks aspartic acid racemase, alanine racemase and glutamic acid racemase activities. The enzyme also does not respond to ATP-[32P]Pi exchange, nor does it catalyse the antibiotic synthesis in presence of amino acids of configuration opposite to that present in the molecule. Preincubation with optical isomers of opposite configuration inhibited the ATP-[32P]Pi exchange reaction to the extent of 60-90%. None of the three fractions of mycobacillin synthetase contained a pantothenic acid arm. Two molecules of ATP are required to synthesize one peptide bond of mycobacillin. Intermediate peptides of mycobacillin are not covalently linked to the three-fraction mycobacillin synthetase.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism*; D009158:Mycobacillin / Q000378:metabolism; D010453:Peptide Synthases / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D011487:Protein Conformation; D019998:Racemases and Epimerases / Q000378:metabolism
D000596:Amino Acids; D009097:Multienzyme Complexes; D010710:Phosphates; D009158:Mycobacillin; D000255:Adenosine Triphosphate; D019998:Racemases and Epimerases; D010453:Peptide Synthases; C042173:mycobacillin synthetase