Effects of growth rate and methotrexate on folate polyglutamates and folylpolyglutamate hydrolase activity in Krebs ascites cells.
The activity of folylpolyglutamate hydrolase was measured throughout intraperitoneal growth of Krebs ascites cells in mice and after exposure to methotrexate. Hydrolase activity was lowest during the log phase of growth. Methotrexate administered intraperitoneally during log growth caused a dose- and time-dependent increase in hydrolase activity. Modest changes were observed in endogenous folate polyglutamate chain length distributions throughout growth and upon exposure to methotrexate, but these changes could not be correlated with hydrolase activity.
D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism*; D002287:Carcinoma, Krebs 2 / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005492:Folic Acid / Q000031:analogs & derivatives*; D007700:Kinetics; D008727:Methotrexate / Q000494:pharmacology*; D051379:Mice; D011624:Pteroylpolyglutamic Acids / Q000378:metabolism*; D011623:gamma-Glutamyl Hydrolase / Q000378:metabolism*
D011624:Pteroylpolyglutamic Acids; D005492:Folic Acid; D002268:Carboxypeptidases; D011623:gamma-Glutamyl Hydrolase; D008727:Methotrexate