One-step extraction and cleanup procedure for determination of p,p'-DDT, p,p'-DDD, and p,p'-DDE in fish.
A simplified method that combines extraction, partitioning, and cleanup in a single step for measuring p,p'-DDT and its metabolites in fish is described. Minced fish samples are emulsified with disodium hydrogen orthophosphate and trisodium citrate, ground with sodium sulfate, and eluted from a chromatographic column prepacked with alumina and silicic acid. The fats and fatty acids are solubilized and easily extracted from the tissues and retained by the column, while p,p'-DDT and its metabolites are quantitatively eluted with 40 mL n-hexane. The eluate is directly applied to a gas chromatographic column. Average recoveries of p,p'-DDT and its metabolites added to fish in vitro are 81%. The average coefficient of variation for recoveries of p,p'-DDT and its metabolites is less than 6.5% and the detection limit is 0.001 micrograms/g for p,p'-DDE, thus making this method very suitable for residue analysis.
D000818:Animals; D002347:Carps / Q000378:metabolism; D002849:Chromatography, Gas; D003634:DDT / Q000032:analysis*; D003633:Dichlorodiphenyl Dichloroethylene / Q000032:analysis*; D003632:Dichlorodiphenyldichloroethane / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D013045:Species Specificity; D014873:Water Pollutants / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D003633:Dichlorodiphenyl Dichloroethylene; D003634:DDT; D003632:Dichlorodiphenyldichloroethane