A theoretical approach to the spectrophotometry of nucleic acids on paper disks.
A linear approximated model is presented that takes into account a local anisotropic distribution of scattered light within a turbid medium. Light intensity depends upon the distance from the boundary surface of the scattering centers, so that the probability of photon-chromophore interaction is a function of the distance. For the sake of simplicity it is assumed that there are only two main zones, corresponding to two probabilities of photon-chromophore interaction. In this form, the Kubelka-Munk theory fits the experimental data supplied by bichromatic photometry of nucleic acids on paper disks.
D002854:Chromatography, Paper / Q000295:instrumentation; D008027:Light; D008433:Mathematics; D008962:Models, Theoretical; D009391:Nephelometry and Turbidimetry; D009696:Nucleic Acids / Q000032:analysis*; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000379:methods
D009696:Nucleic Acids