title: A postmeiotically expressed clone encodes lactate dehydrogenase isozyme X.
abstract: We have analysed by the DNA sequencing one of the cDNA clones, pPM459, to mRNA abundant in spermatids of mice. This clone contained 535 base pair nucleotides with a coding region for 139 amino acids and a 3' untranslated region including a single polyadenylation signal. Screening of the protein database revealed that the deduced amino acid sequence highly matched the carboxyl terminal residues 192-330 of mouse lactate dehydrogenase isozyme X (LDH-X). Taken together with our previous report which showed transcription of the message hybridizing to pPM459 after meiosis, it was demonstrated that LDH-X mRNA synthesis continued during the postmeiotic phase in spermatogenesis.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008297:Male; D008540:Meiosis; D051379:Mice; D009693:Nucleic Acid Hybridization; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics*; D013087:Spermatids / Q000032:analysis; D013091:Spermatogenesis; D013737:Testis / Q000201:enzymology*; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D007770:L-Lactate Dehydrogenase