[Rapid diagnosis of influenza: a comparative evaluation of different methods].
The results of influenza diagnosis during the outbreak of 1985 are presented. Nasopharyngeal secretions from 94 patients were examined by virus isolation in chick embryos, fluorescent antibody technique (FAT). enzyme-immunoassay (EIA), and dot-blot hybridization method (DBHM). The virus was isolated in 28%, FAT was positive in 22%, EIA in 47% of the cases. Among 94 secretion specimens 40 were tested by DBHM. In this instance, virus was isolated in 37%, EIA was positive in 65%, and DBHM in 85% of the cases. It seems advisable to use EIA based on the detection of the type-specific antigen (matrix protein) and DBHM which identifies the serovariant of influenza virus.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002642:Chick Embryo; D004196:Disease Outbreaks / Q000453:epidemiology; D005069:Evaluation Studies as Topic; D006801:Humans; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D008722:Methods; D009031:Moscow; D012698:Serologic Tests / Q000379:methods; D013383:Suburban Population
D000914:Antibodies, Viral
Bystraia diagnostika grippa: sravnitel'naia otsenka razlichnykh metodov.