Involvement of the membrane fluidity of lactosylceramide-targeted liposomes in their intrahepatic uptake.
Incorporation of N-lignoceroyldihydrolactocerebroside (lactosylceramide) enhanced liver uptake of small unilamellar liposomes consisting of dipalmitoylphosphatidylcholine, cholesterol and dicetyl phosphate (molar ratio, 4:5:1). The increase in liver uptake was mostly accounted for by an enhanced uptake into the parenchymal cells. The enhancing effects of lactosylceramide on uptake of the liposomes into liver in vivo and into isolated parenchymal cells in vitro were greater with dipalmitoylphosphatidylcholine-based liposomes than with dimyristoylphosphatidylcholine-based ones. In contrast, addition of lactosylceramide had no significant effect on egg phosphatidylcholine vesicle uptake. The stimulated uptake of lactosylceramide liposomes by parenchymal cells was counteracted by added asialofetuin. These observations suggest that transfer of the targeted liposomes via a galactose-specific receptor into parenchymal cells may be controlled by the membrane fluidity of the liposomes.
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol; D006028:Glycosphingolipids / Q000378:metabolism*; D007700:Kinetics; D007790:Lactosylceramides / Q000378:metabolism*; D008081:Liposomes / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity; D010755:Organophosphates; D011663:Pulmonary Surfactants / Q000008:administration & dosage*; D051381:Rats; D011919:Rats, Inbred Strains
D006028:Glycosphingolipids; D007790:Lactosylceramides; D008081:Liposomes; D010755:Organophosphates; D011663:Pulmonary Surfactants; C002290:dicetylphosphate; D002784:Cholesterol