Biochemical analysis of bovine testicular anti-Müllerian hormone.
Direct biochemical analysis has been applied to bovine testicular anti-Müllerian hormone (AMH), purified from incubation medium of bovine fetal testes by immunochromatography on a monoclonal antibody. The hormone contains a high proportion of hydrophobic amino acids and 13.5% carbohydrate. The oligosaccharide composition suggests that both N- and O-glycosidically linked chains are present. The molecular extinction coefficient is 3.27 +/- 0.06. One RIA unit, defined as the amount of hormone released by 1 g fetal bovine testicular tissue incubated during 4 h, corresponds to 3.06 +/- 0.17 microgram protein.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D054304:Anti-Mullerian Hormone; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D006023:Glycoproteins; D006131:Growth Inhibitors; D008297:Male; D011863:Radioimmunoassay; D013735:Testicular Hormones / Q000032:analysis*; D013737:Testis / Q000032:analysis*
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D006131:Growth Inhibitors; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone