Effect of topical chemical irritation on the blood-aqueous barrier of the rat eye.
The effect of topical neutral formaldehyde on the blood-aqueous barrier of the rat was studied. Thirty minutes after irritation, the protein concentration of the aqueous humour had increased from the control value of 1.95 +/- 0.25 to 2.95 +/- 0.45 mg/ml (p less than 0.05). Extravasation of Evans blue dye from the iris vessels after irritation could be demonstrated by fluorescence microscope. These changes could be eliminated by prior denervation of the trigeminal sensory nerve. The present work demonstrates that the eye of the rat responds to topical chemical irritation. This response is dependent on sensory innervation.
D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D001808:Blood Vessels / Q000378:metabolism; D005123:Eye / Q000098:blood supply*; D005136:Eye Proteins / Q000378:metabolism; D005557:Formaldehyde / Q000494:pharmacology*; D007498:Iris / Q000098:blood supply; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013562:Sympathectomy
D005136:Eye Proteins; D005557:Formaldehyde