Effect of 1-anilinonaphthalene-8-sulphonate on phase transition temperature of dipalmitoylphosphatidylcholine liposomes.
The effect of 1-anilinonaphthalene-8-sulfonate (ANS) on the thermotropic phase transition of dipalmitoylphosphatidylcholine (DPPC) bilayers was examined by differential scanning calorimetry. The main phase transition temperature was found to be shifted to lower values in the presence of the probe. The shift strongly depends on pH and the presence of salts. This indicates that the penetration of the probe of the hydrocarbon moiety of the bilayer is influenced by coulombic interactions. Pretransition phenomena are also affected. The implications for the interpretation of experimental data of biomembrane studies are discussed.
D000817:Anilino Naphthalenesulfonates; D002152:Calorimetry, Differential Scanning; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers; D008954:Models, Biological; D009994:Osmolar Concentration; D011663:Pulmonary Surfactants
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D011663:Pulmonary Surfactants; C027132:1-anilino-8-naphthalenesulfonate