Phytohemagglutinin-conditioned medium modulates adherence properties and morphology of hairy cells.
The effect of a PHA-conditioned medium was tested on a population highly enriched in hairy cells obtained from spleens of patients with hairy cell leukemia. Under PHA-CM treatment hairy cells adhered to both plastic and glass and spread assuming a typical stellate shape, without changing their enzymatic and antigenic properties. Provided PHA-CM was present, stellate adherent hairy cells remained viable in culture for several months. Mononuclear splenic cells from patients with various hematological disorders never showed similar phenotypic modifications after PHA-CM treatment.
D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002448:Cell Adhesion / Q000187:drug effects*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000473:pathology*; D008222:Lymphokines / Q000494:pharmacology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013154:Spleen / Q000473:pathology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D003470:Culture Media; D008222:Lymphokines; D010835:Phytohemagglutinins