A preliminary study on the immunodiagnosis of paragonimiasis--the physico-chemical and immunological characteristics of the crude and partially purified antigens of Paragonimus westermani (Liaoning).
We have now succeeded in preparing a partially purified antigen (Fr. 1). The positive rates of the indirect ELISA were 100% both for sera of proved human cases and dogs infected with Paragonimus westermani when Fr. 1 was used, whereas the positive rates of the test were 71.4% and 75% on sera from patients and infected dogs respectively when crude antigen was used. Moreover, it is also better than the crude antigen in specificity, so a more reliable test in immunodiagnosis of paragonimiasis is available in our laboratory.
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010237:Paragonimiasis / Q000175:diagnosis*; D010238:Paragonimus / Q000276:immunology*
D000947:Antigens, Helminth