Microanalysis of free fatty acids in plasma of experimental animals and humans by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method was developed for microanalysis of thirteen free fatty acids using 200 microliter of plasma. Fatty acids were derivatized with 9-anthryldiazomethane for HPLC analysis. Use of an ODS minicolumn for pretreatment of plasma gave a more accurate determination of free fatty acids in plasma than by chloroform extraction. Using this method, thirteen free fatty acids in the plasma of normal human, dog, rabbit, guinea pig and rat were determined.
D000818:Animals; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid; D005230:Fatty Acids, Nonesterified / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D010716:Phosphatidylinositols / Q000032:analysis; D013045:Species Specificity
D005230:Fatty Acids, Nonesterified; D007202:Indicators and Reagents; D010716:Phosphatidylinositols; D002725:Chloroform