title: Fractionation of Rhizobium leguminosarum cells into outer membrane, cytoplasmic membrane, periplasmic, and cytoplasmic components.
abstract: Rhizobium leguminosarum cells were separated into four distinct fractions by using density gradient centrifugation for the separation of the outer and cytoplasmic membranes and lysozyme-EDTA treatment of whole cells for the isolation of the periplasmic and cytoplasmic fractions. These methods allowed the subcellular localization of R. leguminosarum proteins.
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D012231:Rhizobium / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins