Alteration by concanavalin A of the slow dissociable component in the human growth hormone-receptor interaction.
In mice liver plasma membranes (PM), the binding affinity of receptors for [125I] human growth hormone (hGH) was dependent on the association time: after 18 hours, a high affinity receptor form with KA = 6.8 X 10(9) M-1 accumulated and, as compared to after 1 hour, an increase up to 88%, in a slow dissociating component was observed. Preincubation of PM with concanavalin A (Con A) or other lectins from Lens culinaris (LCA), Ricinus communis (RCA I), Wheat germ agglutinin (WGA) specifically inhibited the binding of hGH to receptors by 54, 28, 50 and 25%, respectively. Furthermore, PM pretreatment with Con A concomitantly increased the rate of dissociation of the hormone-receptor (H-R) complex to 92 or 65% after association for 1 or 18 hours. These Con A-induced alterations resulted from a reduced fraction of the slow dissociable component together with an increased rate constant. The treatment of PM with Con A subsequent to incubation with the hormone did not decrease hormone binding but caused the conversion of the class of hGH receptors exhibiting fast dissociation kinetics towards a form exhibiting slow ones. These data strongly suggest a role for glycoproteins of the N-acetyllactosaminic type in the affinity state of liver membrane hGH receptors.
D000818:Animals; D001667:Binding, Competitive; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins / Q000502:physiology; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000648:ultrastructure; D051379:Mice; D011485:Protein Binding; D011986:Receptors, Somatotropin / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
D006023:Glycoproteins; D007457:Iodine Radioisotopes; D037102:Lectins; D011986:Receptors, Somatotropin; D003208:Concanavalin A