Preparation and immunoreactivity of high specific activity indium-111-DTPA labeled monoclonal antibody (MoAb) using ultrapure indium-111.
The preparation of high-specific activity 111In-DTPA-MoAb without increasing the number of DTPA molecules per Ab was investigated. Instant thin layer chromatography was used to assay the relationship between labeling efficiencies and specific activities. With ultrapurified 111In, the specific activity of the radiolabeled MoAb approached the expected theoretic maximum of 100 muCi/microgram. The bioactivity of such high-specific activity preparation showed no degradation as measured by in vitro cell binding assay.
D000818:Animals; D000911:Antibodies, Monoclonal; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007204:Indium; D007553:Isotope Labeling / Q000379:methods*; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging; D004369:Pentetic Acid; D011786:Quality Control; D011868:Radioisotopes; D011877:Radionuclide Imaging
D000911:Antibodies, Monoclonal; D011868:Radioisotopes; D007204:Indium; D004369:Pentetic Acid