Genetic and chemical factors affecting chemical mutagenesis in cultured mammalian cells.
The genetic and chemical factors affecting chemical mutagenesis in cultured Chinese hamster V79 cells are described. The frequency of mutations induced by the same chemicals at the same concentrations was markedly affected by the genetic markers used. Some chemicals that were positive in the Ames tests induced 8AGr and 6TGr mutations at higher frequencies than OUAr mutations. Carcinogens that were negative in the Ames tests induced only 8AGr mutations, but did not induce OUAr mutations. As a compound affecting mutations induced by EMS, vitamin C showed marked effects in reducing the cytotoxicity and frequency of 6TGr mutations induced by EMS when cells were treated simultaneously with vitamin C at a concentration of 100 micrograms/ml.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005787:Gene Frequency; D007700:Kinetics; D008168:Lung; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D013329:Structure-Activity Relationship
D009153:Mutagens