title: Effect of dilauroylphosphatidylcholine on the acrosome reaction and subsequent penetration of bull spermatozoa into zona-free hamster eggs.
abstract: Incubation of bull sperm with liposomes made with phosphatidylcholine (PC) containing fatty acyl chains of either 10 (PC10) or 12 (PC12) carbons resulted in greater than 90% of the sperm exhibiting an acrosome reaction (AR) within 15 min. Liposomes of PC10 rapidly destroyed sperm motility while PC12 acrosome-reacted sperm remained motile for several h. Liposomes of PC with greater than or equal to 14-carbon fatty acyl chains had no effect on the AR or motility of sperm. The AR was not induced by lysophospholipids, because lysophospholipids were not detected in the PC liposomes, and the AR did not occur when lysophospholipids were tested at the same concentration as PC12. The concentration of PC12 necessary to induce maximal numbers of acrosome-reacted sperm varied with the concentration of sperm. The effect of PC12 on sperm also varied with the ratio of live to dead sperm in a sample. When 3 X 10(6) bull sperm/ml were treated with 0, 10, 20, and 30 microM PC12 for 7 min prior to addition to zona-free hamster eggs, 6, 6, 98, and 77% of the eggs were penetrated, respectively. Lipid concentrations of 0 microM and 10 microM did not affect the AR, whereas higher levels induced the AR in sperm. This procedure can quickly provide acrosome-reacted bull sperm for use with various in vitro fertilization procedures and for assessment of male fertility.
mesh_terms: D000177:Acrosome / Q000187:drug effects*; D000818:Animals; D002417:Cattle; D006224:Cricetinae; D005260:Female; D066298:In Vitro Techniques; D008081:Liposomes; D008297:Male; D008561:Membrane Fusion / Q000187:drug effects; D010713:Phosphatidylcholines / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; C015153:1,2-dilauroylphosphatidylcholine