Glucose ameliorates the depletion of NADPH by paraquat in rat lung slices.
Paraquat-stimulated NADPH depletion in rat lung slices is responsive to exogenous glucose concentration. Lung slices incubated with 11 mM glucose and 10(-4) M paraquat had a 40% lower NADPH/NADP+ ratio than did control lung slices. Incubation with no added glucose and 10(-5) M paraquat caused a 41% decrease in NADPH/NADP+. With paraquat at 10(-5) M, glucose at 1.1, 5.5, 11, or 22 mM increased NADPH/NADP+ ratios in a concentration-dependent manner until, at 22 mM glucose, the effect of paraquat was prevented. The sum of NADP+ plus NADPH was only 60% of control with 10(-5) M paraquat and no glucose. However, with any concentration of glucose from 1.1 to 22 mM, the total was 92% of control. The results indicate that glucose may be beneficial in preventing paraquat-mediated NADPH depletion in rat lung slices.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D004247:DNA / Q000032:analysis; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D008168:Lung / Q000032:analysis / Q000187:drug effects*; D008297:Male; D009249:NADP / Q000032:analysis*; D010269:Paraquat / Q000633:toxicity*; D051381:Rats
D009249:NADP; D004247:DNA; D005947:Glucose; D010269:Paraquat