Determination of clobazam and its N-demethyl metabolite in serum of epileptic patients.
We describe a gas-liquid chromatographic method, using a nitrogen-specific detector, which is suitable for the simultaneous quantitation of clobazam and its main metabolite N-demethyl clobazam in the serum of epileptic patients treated with other anticonvulsant co-medication. Flunitrazepam (internal standard) is added to the sample and after extraction with a toluene/ethyl acetate mixture (3 + 1 by vol), the organic extract is evaporated and the residue is reconstituted in a small volume of solvent and chromatographed on a 3% SP2250 column. The sensitivity limits are about 2 to 5 micrograms per liter of original sample.
D014151:Anti-Anxiety Agents; D000927:Anticonvulsants / Q000097:blood*; D001569:Benzodiazepines; D001570:Benzodiazepinones / Q000097:blood* / Q000627:therapeutic use; D002849:Chromatography, Gas / Q000379:methods; D000078306:Clobazam; D004827:Epilepsy / Q000097:blood / Q000188:drug therapy*; D006801:Humans
D014151:Anti-Anxiety Agents; D000927:Anticonvulsants; D001570:Benzodiazepinones; D001569:Benzodiazepines; D000078306:Clobazam; C024581:N-desmethylclobazam