title: Cation transport in lung epithelial cells derived from fetal, newborn, and adult rabbits.
abstract: Recent studies done with fetal and adult sheep and with monolayers of cultured rat alveolar type II cells suggest that active transport of Na+ across the lung epithelium may contribute to liquid absorption from air spaces, an essential component of the normal switch from placental to pulmonary gas exchange at birth. The goals of this work were 1) to study the ontogeny of cation transport in lung epithelial cells derived from fetal, newborn, and adult rabbits and 2) to determine the influence of premature birth, air breathing, labor, and postnatal lung maturation on K+ uptake in these cells. We harvested granular pneumonocytes by tracheal instillation of proteolytic enzymes followed by centrifugation of the dispersed cells over a discontinuous density gradient of metrizamide. This procedure yielded 65-90% granular pneumonocytes, of which more than 80% excluded vital dye. Using freshly isolated cells, we measured uptake of 86Rb+, which mimics transmembrane movement of K+, in the presence or absence of 10(-4) M ouabain and in the presence or absence of 5 X 10(-4) M furosemide or bumetanide. In adult rabbit studies, 86Rb+ uptake was twice as fast in lung epithelial cells (98 +/- 7 nmol X 10(6) cells-1 X h-1) as it was in alveolar macrophages (51 +/- 6 nmol X 10(6) cells-1 X h-1). Ouabain inhibited 55-60% of the uptake by pneumonocytes, and "loop" diuretics inhibited an additional 15-20%. The rate of 86Rb+ uptake in fetal cells was less than 10% (6 +/- 1 nmol X 10(6) cells-1 X h-1) of the rate in adult cells; ouabain inhibited 80-85% of 86Rb+ uptake in fetal cells.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001692:Biological Transport; D036861:Delivery, Obstetric; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005865:Gestational Age; D006098:Granulocytes / Q000378:metabolism; D007474:Ion Exchange; D007743:Labor, Obstetric; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D010101:Oxygen Consumption; D011188:Potassium / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits; D011868:Radioisotopes; D012413:Rubidium; D012964:Sodium / Q000378:metabolism
chemical_list: D011868:Radioisotopes; D012964:Sodium; D012413:Rubidium; D011188:Potassium