title: Isolation and characterization of cephalothin-susceptible Campylobacter coli from slaughter cattle.
abstract: In a recent meat survey, 10 of 13 (77%) Campylobacter coli isolates were susceptible to cephalothin. These organisms were isolated from nine slaughter cattle from eight meat packing establishments. All 10 isolates grew at 43 degrees C but not at 25 degrees C, were catalase and oxidase positive, and were susceptible to nalidixic acid (30 micrograms) and cephalothin (30 micrograms). The cultures were subsequently identified as C. coli serogroup 20, biotype I (Lior scheme). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that protein and lipopolysaccharide profiles of whole cell preparations of the 10 cephalothin-susceptible strains and the reference strain for C. coli serogroup 20 were very similar. The plasmid profiles of these 11 strains were identical.
mesh_terms: D000003:Abattoirs; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002167:Campylobacter / Q000032:analysis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle / Q000382:microbiology*; D002512:Cephalothin / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis; D008826:Microbial Sensitivity Tests; D010957:Plasmids
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D002512:Cephalothin