A study of drug binding to DNA by partial intercalation using a model phenazine derivative.
Spectrophotometry, thermal denaturation, sedimentation, and viscometric techniques were used in a study of interaction of double helical DNA with an asymmetric phenazinium derivative, aposafranine. The results obtained indicate that aposafranine binds to DNA by a single binding mode, a wedge-like partial intercalation.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D004247:DNA / Q000187:drug effects*; D007364:Intercalating Agents / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D010619:Phenazines / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods; D014783:Viscosity
D007364:Intercalating Agents; D010619:Phenazines; C050238:aposafranine; D004247:DNA