Osteodentin formation in rat incisor as visualized by radioautography after 3H-proline administration.
Osteodentin formation was studied in rat incisor pulp after adriamycin administration. Male Sprague Dawley rats (100 +/- 5 gm) were injected intravenously with adriamycin (5 mg/kg body weight), and after 7 days they were again injected intravenously with 3H-proline (3 microCi/gm). These animals were killed in groups of three from 5 minutes to 4 hours after proline injection by perfusion with 3% phosphate-buffered formaldehyde followed by 2.5% phosphate-buffered glutaraldehyde. Control animals injected with only physiological saline, and 7 days later with 3H-proline (3 microCi/gm), and were killed at the same time intervals. Radioautography on sections showing osteodentin formation revealed that at 5 minutes after 3H-proline injection the labeling was located over the cells associated with the osteodentin matrix. At 1 hour after injection the labeling was located over the cells and the matrix, while at 4 hours the labeling was seen only over the matrix. It therefore appears that at least a proline-containing component of the osteodentin matrix is synthesized and secreted by the cells associated with it.
D000818:Animals; D003782:Dental Pulp / Q000187:drug effects / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism*; D004317:Doxorubicin / Q000494:pharmacology; D008297:Male; D011392:Proline / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004317:Doxorubicin; D011392:Proline