title: [Genotypic and phenotypic characteristics of L-line cells resistant to ethidium bromide].
abstract: Stable mutants resistant to ethidium bromide in concentrations of 1 and 3 micrograms/ml have been selected in a single step in L cells. The frequency of spontaneously occurring ethidium bromide resistant clones after the exposure to 1 microgram/ml of the drug has been established as 5.10(-5). Resistant variants were induced following treatment with mutagen N-methyl-N-nitro-N-nitrosoguanidine. The resistant clones were shown to be resistant to higher concentration of the agent then which was used for selection. In multistep selection, a number of clones resistant to ethidium bromide in concentration up to 50 micrograms/ml was obtained. The alteration in the permeability of plasma membrane to the drug is the clue mechanism of the resistance.
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004996:Ethidium / Q000037:antagonists & inhibitors*; D005838:Genotype; D007739:L Cells / Q000187:drug effects*; D051379:Mice; D009153:Mutagens / Q000494:pharmacology; D010641:Phenotype; D012641:Selection, Genetic
chemical_list: D009153:Mutagens; D004996:Ethidium
vernacular_title: Genotipicheskie i fenotipicheskie osobennosti keltok linii L, ustoĭchivykh k bromistomu étidiu.