A rat epididymal sialoglycoprotein with alpha-lactalbumin activity.
A major sialoglycoprotein from rat epididymal fluid was radiolabeled by NaIO4/[3H]KBH4 method and purified by chromatography using DEAE-Sepharose and chromatofocusing columns. Its Mr was 21,000 and its pI value was 4.9. Since it possessed a high sialic acid content and an alpha-lactalbumin-like activity, it was proposed to be a sialylated form of epididymal alpha-lactalbumin-like protein.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000378:metabolism*; D007768:Lactalbumin / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000032:analysis; D012795:Sialoglycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D011506:Proteins; D012794:Sialic Acids; D012795:Sialoglycoproteins; D007768:Lactalbumin