Use of a rapid and highly sensitive fluorescamine-based procedure for the assay of plasma lipoproteins.
A rapid and sensitive method for determining protein concentrations using fluorescamine has been characterized for use in the analysis of intact lipoproteins. It was shown that there is no interference with the assay due to the presence of lipid-associated turbidity or primary amine content. The assay was shown to be sensitive to as little as 0.3 microgram of lipoprotein and to yield similar results when compared to the Lowry method.
D003124:Colorimetry / Q000379:methods; D005450:Fluorescamine; D006801:Humans; D007457:Iodine Radioisotopes; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D012016:Reference Values; D013050:Spectrometry, Fluorescence / Q000379:methods
D007457:Iodine Radioisotopes; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D005450:Fluorescamine