A new microcirculation chamber for inexpensive long-term investigations of nervous tissue in vitro.
The construction of a simple universal chamber for long-term recording from submerged and interface brain slices is described. The medium is circulated and oxygenated constantly by an O2-CO2 gas mixture using the principle of a bubble pump. The design permits experiments which require long-term exposure to expensive drugs as well as great economy of oxygen consumption.
D000818:Animals; D001921:Brain; D003365:Costs and Cost Analysis; D004867:Equipment Design; D066298:In Vitro Techniques; D010477:Perfusion / Q000379:methods; D013997:Time Factors; D014021:Tissue Preservation / Q000295:instrumentation*