A cartilage-derived growth factor enhances hyaluronate synthesis and diminishes sulfated glycosaminoglycan synthesis in chondrocytes.
Cartilage-derived growth factor purified to homogeneity by affinity chromatography on columns of heparin-Sepharose was mitogenic for early passage bovine fetal chondrocytes. Hyaluronate and sulfated glycosaminoglycan synthesis in these cells was analyzed by differential enzymatic digestion of the glycosaminoglycans labeled with [14C] glucosamine or [35S]. It was found that chondrocyte proliferation was accompanied by about a four-fold increase in hyaluronate synthesis over a two-day period, while the synthesis of sulfated glycosaminoglycans decreased by about 2-fold. Chromatographic analysis of the sulfated glycosaminoglycans showed decreases in chondroitin 4 and 6 sulfates. It was concluded from these results that cartilage-derived growth factor was a proliferative factor for chondrocytes and differed from the somatomedins.
D000818:Animals; D001345:Autoradiography; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002855:Chromatography, Thin Layer; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D010641:Phenotype; D011506:Proteins / Q000494:pharmacology*
D006025:Glycosaminoglycans; D011506:Proteins; C013786:A73025; D006820:Hyaluronic Acid