Percutaneous inoculation of lymph nodes.
Injections into rat lymph nodes are easily performed percutaneously, without the need for a skin incision, if the nodes are enlarged in advance. The requisite enlargement of the popliteal lymph node can be obtained in 2 or 3 weeks by a single injection of tin powder or other particulate material into the foot. This method may be especially useful when multiple intranodal inoculations are contemplated.
D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology; D007271:Injections, Intradermal; D007272:Injections, Intralymphatic; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D007717:Knee; D008198:Lymph Nodes; D010567:Pertussis Vaccine / Q000008:administration & dosage; D011292:Premedication; D051381:Rats; D014001:Tin / Q000008:administration & dosage
D010567:Pertussis Vaccine; D014001:Tin