Microconductimetric assay of butyrylcholinesterase activity.
A conductimetric method for the assay of butyrylcholinesterase is described. A new conductimetric cell has been used which allows increased sensitivity. The physicochemical parameters (pH, buffer concentration) have been optimized. With 1 mM butyrylcholine, butyrylcholinesterase activities down to 2 x 10(-4) U ml-1 may be measured. Serum volumes needed for this assay are in the microliter range (1-5 microliters).
D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood*; D002802:Cholinesterases / Q000097:blood*; D003217:Conductometry / Q000295:instrumentation / Q000379:methods*; D006736:Horses; D006868:Hydrolysis; D008832:Microchemistry / Q000379:methods*
D002091:Butyrylcholinesterase; D002802:Cholinesterases