Monitoring of intracellular Ca2+ elevation in a single neural cell using a fluorescence microscope/video-camera system.
For monitoring the changes in intracellular free Ca2+ concentration ([Ca2+]i), we developed a simple system combining a fluorescence microscope, an image intensifier, a video-camera, a cathode ray tube display and a photodiode, employing quin2 as a Ca2+ indicator. We recorded increases of the fluorescence intensity due to [Ca2+]i rises, when high K+ medium, neurotransmitter and Ca2+ ionophore were applied to the single cells of nervous system origin in culture. The present system is capable of simultaneous detection of the [Ca2+]i changes from multiple separate cells.
D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000032:analysis*; D051379:Mice; D008856:Microscopy, Fluorescence; D008991:Monitoring, Physiologic / Q000379:methods; D009474:Neurons / Q000032:analysis*; D051381:Rats; D013050:Spectrometry, Fluorescence; D014743:Videotape Recording
D000634:Aminoquinolines; C035739:Quin2; D002118:Calcium