[Effect of ascorbic acid on the free radicals stimulating an antigen-antibody reaction in a model system of spermatozoa-antispermatozoal serum].
Studied was the effect of ascorbic acid in a model antigen-antibody test with a spermatozoa-antispermatozoid serum system, using four values of acidum ascorbinicum as a widely occurring reduction agent in nature on another reducing agent, cysteine, as the source of free SH groups (up to 94 per cent of the SH groups of cysteine are released in a free radical state). Ascertained was the stimulating role of the SH groups, being the active part of the cysteine molecule. With raising the concentration of ascorbic acid from 0.0001 M to 0.001 M, 0.01 M, and 0.1 M it inhibited progressively the stimulating activity of the SH groups in the antigen-antibody test with the model system. The rates within the range of up to 0.01 M could as well produce a suppressive effect on certain immune reactions with no harm for the body. One of the reasons for such an effect could be traced back to the presence of a negative charge of the ascorbic acid molecule, which blocked the activity of the SH groups and played a part in their thermodynamic behaviour as well as to the higher redox potential of the ascorbic acid as compared to that of cysteine.
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects*; D001205:Ascorbic Acid / Q000494:pharmacology*; D003545:Cysteine / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D007106:Immune Sera / Q000276:immunology*; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D012756:Sheep; D013094:Spermatozoa / Q000187:drug effects* / Q000276:immunology; D013438:Sulfhydryl Compounds / Q000276:immunology
D005609:Free Radicals; D007106:Immune Sera; D013438:Sulfhydryl Compounds; D003545:Cysteine; D001205:Ascorbic Acid
Vliianie na askorbinovata kiselina vŭrkhu svobodni radikali, stimulirashti reaktsiiata antigen/antitialo na modelna sistema spermatozoidi/ antispermatozoiden serum.