The immunogenic properties of poly(dI).poly(dC) and poly(rI). poly(dC)--analysis by monoclonal antibodies.
Eight monoclonal antibodies were prepared from a C57/black mouse which had been immunized with poly(rI).poly(dC). Two of the antibodies were specific for the RNA-DNA duplex but bound about 100-fold better to poly(rI).poly(dC) than to poly(rA).poly(dT). The other six antibodies were single-strand specific and bound to poly(rI) and in most cases to poly(dI) as well, but not to other single-stranded nucleic acids. Similarly, 10 monoclonal antibodies were produced from mice immunized with poly(dI).poly(dC). Nine of these were specific for poly(dI) while the other had a strong preference for poly(dC). Thus, in contrast to poly(rI).poly(dC), the all DNA duplex poly(dI). poly(dC) only elicits duplex specific antibodies at very low frequency, if at all.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D001667:Binding, Competitive; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011089:Polydeoxyribonucleotides / Q000276:immunology*; D011119:Polynucleotides / Q000276:immunology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D011089:Polydeoxyribonucleotides; D011119:Polynucleotides; C031156:poly d(I-C); C049667:poly(rI).poly(dC)