Protein and cDNA sequence of a glycine-rich, dimethylarginine-containing region located near the carboxyl-terminal end of nucleolin (C23 and 100 kDa).
By a combination of protein chemistry and recombinant DNA methods a glycine-rich region was found to be located near the carboxyl terminus of the nucleolar specific phosphoprotein, nucleolin, from Novikoff hepatoma (protein C23) and Chinese hamster ovary cells (100-kDa nucleolar protein). A sequence of 192 amino acid residues was derived from partial sequences of cyanogen bromide and N-bromosuccinimide fragments of protein C23 and deduced protein sequence from Chinese hamster ovary cell 100-kDa cDNA sequences. The 66 residues sequenced by protein methods were identical to the corresponding residues deduced by DNA sequencing. The multiple residues of NG,NG-dimethylarginine (DMA) contained in the nucleolin polypeptide were found to be limited to a segment of less than 10 kDa near the carboxyl-terminal end of the protein. This segment also contained internally repeated sequences (e.g. 7 copies of the sequence Gly-Gly-Arg-Gly-Gly were found) which were unrelated to sequences closer to the amino-terminal end. Most arginine residues in this region were surrounded by 2 or 3 glycine residues and were relatively close in sequence to phenylalanine residues.
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D001483:Base Sequence; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA; D004274:DNA, Recombinant; D005260:Female; D005998:Glycine / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D009698:Nucleoproteins / Q000032:analysis / Q000235:genetics; D010053:Ovary / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis / Q000235:genetics; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics; D011499:Protein Processing, Post-Translational; D016601:RNA-Binding Proteins; D000097796:Nucleolin
D001120:Arginine; D004247:DNA; D004274:DNA, Recombinant; D005998:Glycine; D009698:Nucleoproteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C018524:N,N-dimethylarginine