title: Rapid expression of novel proteins in goldfish retina following optic nerve crush.
abstract: Polyadenylated messenger RNA was isolated from goldfish retinas at various times following unilateral crush of the optic nerve. RNA was translated in a cell-free system and product proteins analyzed by two-dimensional gel electrophoresis and autofluorography. Poly(A)+ mRNA-directed protein synthesis revealed an 8-fold increase in the labeling of polypeptides of about 30 kd Mr and a pI of 5.5 in retinas 2 d following optic nerve crush, compared with control retina mRNA translation products. In vitro labeling of retinal proteins revealed the enhanced synthesis of comparable 30 kd proteins in 2 d post-crush retinas. Evidence presented suggests that this 30 kd protein cluster may correspond to fish 30 kd stress or heat-shock proteins (hsp-30).
mesh_terms: D000818:Animals; D006054:Goldfish; D006360:Heat-Shock Proteins / Q000378:metabolism; D007526:Isoelectric Point; D008970:Molecular Weight; D009409:Nerve Crush; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009900:Optic Nerve / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D013997:Time Factors
chemical_list: D006360:Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger