Glucocorticoids change the ratio of type III to type I procollagen extracellularly.
Rat skin fibroblasts were treated with dexamethasone and labeled with radioactive proline for 2 hours. Intracellular and extracellular procollagens were immunoprecipitated with monospecific polyclonal antibodies. Both intracellular and extracellular types I and III procollagen were decreased coordinately by glucocorticoid treatment. The ratio of type III to type I procollagen was the same for control and dexamethasone-treated cell cultures. However, when rat skin fibroblasts were prelabeled with radioactive proline and chased in the presence of unlabeled proline the ratio of type III to type I extracellular procollagens remained constant in the glucocorticoid-treated cells and increased in control fibroblasts.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D011347:Procollagen / Q000096:biosynthesis*; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000378:metabolism*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D011347:Procollagen; D003907:Dexamethasone