title: Use of small charcoal/alumina cleanup columns in determination of trichothecene mycotoxins in foods and feeds.
abstract: Small charcoal/alumina cleanup columns have been effectively used to remove interfering materials from grain, feed, and food extracts prior to chromatographic determination of trichothecene mycotoxins. A thin layer chromatographic method has been developed that can simultaneously detect part per billion concentrations of deoxynivalenol, fusarenon X, nivalenol, T-2 toxin, HT-2 toxin, neosolaniol, and diacetoxyscirpenol in food and feed samples. Recoveries of 90-99% can be obtained. The use of charcoal/alumina cleanup columns in conjunction with liquid chromatography and gas chromatography of trichothecenes is also discussed.
mesh_terms: D000537:Aluminum Oxide; D000821:Animal Feed / Q000032:analysis*; D002606:Charcoal; D002855:Chromatography, Thin Layer; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D009183:Mycotoxins / Q000032:analysis*; D012717:Sesquiterpenes / Q000032:analysis*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014255:Trichothecenes / Q000032:analysis*; D003313:Zea mays / Q000032:analysis
chemical_list: D009183:Mycotoxins; D012717:Sesquiterpenes; D012997:Solvents; D014255:Trichothecenes; D002606:Charcoal; D000537:Aluminum Oxide