The expression of ras oncogene in normal and pathological liver disease.
Activation of the ras oncogene is associated with overproduction of the normal gene product (p21). Ninety one paraffin-embedded specimens were used to map the distribution of the normal form of p21 in normal, inflamed, cirrhotic and carcinomatous liver parenchyma. Monoclonal antibodies (Mo-RAP) were raised against the normal form of the ras-oncogene product and histological sections were stained by the peroxidase-antiperoxidase technique. Normal, inflamed and cirrhotic liver showed either minimal or moderate cytoplasmic staining. By contrast primary (n = 13) and secondary (n = 41) liver carcinomas exhibited intense staining. The differential pattern observed in p21 distribution could have useful clinical applications.
D000911:Antibodies, Monoclonal; D005786:Gene Expression Regulation; D006801:Humans; D008099:Liver / Q000032:analysis*; D008107:Liver Diseases / Q000235:genetics* / Q000473:pathology; D008113:Liver Neoplasms / Q000032:analysis / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D009857:Oncogenes
D000911:Antibodies, Monoclonal; D009856:Oncogene Proteins, Viral