Degradation of 4-hydroxyphenylacetate by Xanthobacter 124X. Physiological resemblance with other gram-negative bacteria.
Xanthobacter 124X when grom on 4-hydroxyphenylacetate was able to hydroxylate this compound yielding homogenisate. Ring fission of this latter compound gave maleylacetoacetate which was isomerized to fumarylacetoacetate. The isomerase involved resembled maleylacetoacetate isomerases in Gram-negative bacteria in that glutathione was required for activity. Fumarate and acetoacetate were both detected as products of the hydrolysis of fumarylacetoacetate.
D002474:Cell-Free System; D049308:Dioxygenases; D006088:Gram-Negative Aerobic Bacteria / Q000378:metabolism*; D050560:Homogentisate 1,2-Dioxygenase; D007535:Isomerases / Q000378:metabolism; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D010648:Phenylacetates / Q000378:metabolism*; D013045:Species Specificity; D019745:cis-trans-Isomerases
D010648:Phenylacetates; C026246:4-hydroxyphenylacetate; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D049308:Dioxygenases; D050560:Homogentisate 1,2-Dioxygenase; C026252:4-hydroxyphenylacetate 3-monooxygenase; D007535:Isomerases; D019745:cis-trans-Isomerases; C021531:maleylacetoacetate isomerase