[Production of a cell line of embryonic rat fibroblasts after transfection with the DNA from the early region of simian adenovirus SA7].
Transfection of plasmid DNA, containing coding sequences for the early region of simian adenovirus SA7, into primary cultures of the rat embryonic fibroblasts results in the establishment of a stable cell line REF-1. The cells have been passed more than 40 times (about 160 doublings) without reaching the critical stage. Their growth is substrate- and concentration-dependent.
D000256:Adenoviridae / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics*; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology; D010957:Plasmids; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection
D004279:DNA, Viral
Poluchenie kletochnoĭ linii émbrional'nykh fibroblastov krys posle transfektsii DNK ranneĭ oblasti adenovirusa obez'ian SA7.