title: Optimization of the production of hCG beta-like material by CaSki human cervical carcinoma cells in roller bottle culture.
abstract: This study was aimed at optimizing large-scale roller bottle culture conditions for CaSki human cervical carcinoma cells, to produce ectopic hCG beta-like material in quantities sufficient for subsequent characterization studies. Several cell culture techniques contributed to the achievement of this goal: (1) use of serum-free culture medium; (2) use of intermittent recovery periods in presence of serum; and (3) ultrafiltration of the serum-free medium pool for initial concentration of 100-fold.
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D003470:Culture Media; D005260:Female; D006801:Humans; D008722:Methods; D011863:Radioimmunoassay; D014462:Ultrafiltration; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D003470:Culture Media