A microcomputer-based image analyzer for quantitating neurite outgrowth.
An automated device that recognizes and quantitates neurite outgrowth from goldfish retinal explants is described. The system components include a video camera-inverted microscope and an Apple II microcomputer with an attached Motorola 68000 coprocessor. Values for neurite outgrowth were generated within 30 s, required no user input other than defining the microscopic field, and correlated well with a subjective measure, the nerve growth index.
D000818:Animals; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003201:Computers; D006054:Goldfish; D008838:Microcomputers; D009474:Neurons / Q000166:cytology*; D012160:Retina / Q000166:cytology* / Q000254:growth & development; D012984:Software