An enzyme-linked immunosorbent assay for the quantitation of human IgG subclasses using monoclonal antibodies.
An enzyme-linked immunosorbent assay was established for the quantitation of human IgG1, IgG2, IgG3 and IgG4 using IgG subclass-specific monoclonal antibodies. The method could detect 1-10 ng/ml of the Ig subclasses. The technique is suitable for measuring IgG subclass concentration in sera of healthy adults and in supernatants from human lymphocytes cultured in the presence of pokeweed mitogen.
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G