Tissue porphyrin pattern determination by high-speed high-performance liquid chromatography.
A rapid and sensitive method for the determination of porphyrin carboxylic acids in liver, kidney, and spleen by high-speed high-performance liquid chromatography is described. Porphyrins were extracted with recoveries greater than or equal to 98%, concentrated on disposable octadecylsilyl cartridges, and analyzed with a liquid chromatograph equipped with a 3 microns X 3 cm octadecylsilyl column and a fluorescence detector. Separation of di-, tetra-, penta-, hexa-, hepta-, and octacarboxylic acids was achieved within 5 min. The detection limits for uro, copro, and protoporphyrin were 20, 10, and 20 fmol, respectively.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D011164:Porphyrias / Q000378:metabolism; D011166:Porphyrins / Q000032:analysis* / Q000592:standards; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards; D013154:Spleen / Q000032:analysis
D011166:Porphyrins