Sensitive method for the determination of 6-oxo-prostaglandin F1 alpha by gas chromatography with electron-capture and mass-fragmentographic detection.
A selective and sensitive quantitative method is described for the determination of the endogenous prostacyclin content of the aorta of rats, treated with GYKI-11679, a new hydrazone derivative with antihypertensive action. The thoracal aorta from three rats was excised and pooled, 1 h after treatment with 10 mg/kg of GY-KI-11679. The prostacyclin, transformed to 6-oxo-PGF1 alpha, was extracted. For gas chromatographic electron-capture detection (GC-ECD), 6-oxo-PGF1 alpha was converted into the O-methyloxime tris(trifluoroacetyl) hexafluoroisopropyl ester derivative. The GC-ECD properties, stability and mass-spectrometric characteristics of this new derivative allow the quantitative analysis of 6-oxo-PGF1 alpha by using nor-PGF2 alpha as internal standard.
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis*; D000818:Animals; D001013:Aorta, Thoracic / Q000032:analysis; D002849:Chromatography, Gas; D004563:Electrochemistry; D008401:Gas Chromatography-Mass Spectrometry; D009131:Muscle, Smooth, Vascular / Q000032:analysis; D051381:Rats
D015121:6-Ketoprostaglandin F1 alpha