The excimer fluorescence of N-(1-pyrenyl)iodoacetamide labeled to myosin and its subfragment 1.
Myosin and its subfragment 1 were labeled with the fluorescent probe N-(1-pyrenyl)iodoacetamide. Both of the labeled complexes exhibited the excimer band at 480 nm (pH 8.0, 25 degrees C). SH1 and SH2 are labeled with this probe as judged by Ca2+-ATPase of the labeled complex. Excimers arise both from the interaction of PIAAs in the two different heads within a single myosin molecule and also from the interaction of PIAAs in the same head. ATP affects these excimers depending on the concentration of Ca2+.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D007460:Iodoacetamide / Q000031:analogs & derivatives*; D007461:Iodoacetates; D015879:Myosin Subfragments; D009218:Myosins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D011817:Rabbits; D013050:Spectrometry, Fluorescence
D007461:Iodoacetates; D015879:Myosin Subfragments; D010446:Peptide Fragments; C029358:N-(1-pyrenyl)iodoacetamide; D000255:Adenosine Triphosphate; D009218:Myosins; D007460:Iodoacetamide