[Synthesis and membrane-dependent processing of a precursor of tick-borne encephalitis virus (flavivirus) structural proteins in cell-free systems].
Conditions that permitted cell-free synthesis of at least one of the non-structural, in addition to two-structural, polypeptides of tick-borne encephalitis virus have been found. The time course of accumulation of virus-specific polypeptides in extracts of Krebs-2 cells and reticulocyte lysates as well as the peptide maps of the products synthesised were studied. A model of generation of viral structural polypeptides has been proposed, according to which a common precursor of these proteins while in a nascent form, is processed in a membrane-dependent reaction into a C-terminal segment, corresponding to the polypeptide moiety of envelope glycoprotein E, and an N-terminal segment, doublet p36/33. Subsequently, an N-terminal segment, corresponding to the core polypeptide C, is cleaved off from p36/33. The remaining C-terminal segment of p36/33 is possibly a precursor of the membrane polypeptide M. The translational strategy of flaviviruses is compared to that of other positive-stranded RNA viruses.
D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism / Q000382:microbiology; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D015678:Viral Structural Proteins
D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins
Sintez i membran-zavisimyĭ protsessing predshestvennika strukturnykh belkov virusa kleshchevogo éntsefalita (flavivirusa) v beskletochnykh sistemakh.