[An ex-vivo technic for the pharmacologic evaluation of potential antiphlogistics by ex-vivo determination of the rate of malondialdehyde (MDA) formation using rat platelets].
Based on a standardized in-vitro method for quantifying the activity of prostaglandin-synthetase by means of coupling malondialdehyde with 2-thiobarbituric acid the possibility of using this method also as ex-vivo technique is described. By this, more favourable prerequisites to pharmacological valuation of the effects of potential antiinflammatory substances exists compared to the application of in-vitro results, only.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D003864:Depression, Chemical; D004008:Diclofenac / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D007052:Ibuprofen / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008314:Malonates / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism*; D051381:Rats
D000893:Anti-Inflammatory Agents; D008314:Malonates; D004008:Diclofenac; D008315:Malondialdehyde; D001241:Aspirin; D007052:Ibuprofen
Ein ex-vivo-Verfahren zur pharmakologischen Bewertung von potentiellen Antiphlogistica anhand der ex-vivo-Bestimmung der Bildungsrate von Malondialdehyd (MDA) unter Verwendung von Ratten-Thrombozyten.