Electron capture gas chromatographic determination of traces of formaldehyde in milk as the 2,4-dinitrophenylhydrazone.
A quantitative method is described for the determination of formaldehyde in milk by packed-column gas chromatography and electron capture detection. Aldehyde derivatization was carried out in situ with 2,4-dinitrophenylhydrazine followed by extraction and analysis using an external standard. Average recoveries of 96.3 +/- 1.6% were characteristic of the chromatographic method with an estimated detection limit of 0.026 mg/kg. The technique was applied to determination of formaldehyde in milk from cows consuming a formalin-treated feedstuff.
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D004563:Electrochemistry; D005557:Formaldehyde / Q000032:analysis*; D006835:Hydrazones / Q000032:analysis*; D007202:Indicators and Reagents; D008892:Milk / Q000032:analysis*
C001072:2,4-dinitrophenylhydrazone; D006835:Hydrazones; D007202:Indicators and Reagents; D005557:Formaldehyde