Ultrastructural organization of choline-acetyltransferase-immunoreactive fibres innervating the neocortex from embryonic ventral forebrain grafts.
Suspension grafts of foetal tissue rich in cholinergic neurones were transplanted into the frontoparietal cortex of rats that had previously undergone deafferentation of the extrinsic cholinergic innervation of the cortex by injection of ibotenic acid into the nucleus basalis magnocellularis. The cortical tissue containing the graft was processed for electron microscopic immunocytochemistry by using a monoclonal antibody to choline acetyltransferase (ChAT) in order to examine the contacts established between cholinergic fibres from the graft and the host neocortex. The density, distribution, and targets of this graft-host innervation were compared with those seen in the intact and deafferented cortex. ChAT-positive fibres in both grafted and control animals formed extensive synaptic connections with various cortical neural elements--those of graft origin being of similar morphology to those in the intact cortex. However, the distribution of postsynaptic cortical targets of the graft-derived ChAT-immunoreactive boutons was abnormal, such that a greater percentage of such terminals formed synaptic contacts with neuronal perikarya, especially layer V pyramidal neurones, than was seen in control brains. It is possible that the formation of new synaptic contacts between the embryonic graft and host frontoparietal cortex may, in part, be necessary for the restoration of functional activity that has been previously reported in these grafted animals.
D000818:Animals; D002540:Cerebral Cortex / Q000648:ultrastructure*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D002799:Cholinergic Fibers / Q000648:ultrastructure; D004622:Embryo, Mammalian; D005260:Female; D007395:Interneurons / Q000648:ultrastructure; D008854:Microscopy, Electron; D009417:Nerve Tissue / Q000637:transplantation*; D051381:Rats; D011919:Rats, Inbred Strains
D002795:Choline O-Acetyltransferase