title: Antigenic analysis of hematopoiesis. V. Characterization of My-10 antigen expression by normal lymphohematopoietic progenitor cells.
abstract: The My-10 glycoprotein is an hematopoietic cell surface antigen expressed specifically by undifferentiated (blast) cells, constituting 1%-4% of normal adult bone marrow leukocytes. We used several immunological and in vitro culture methods to analyze the expression of this unique antigen on a variety of lymphohematopoietic progenitor cells. Colony-forming cells (CFC) for granulocyte-monocyte colonies (CFC-GM) and erythroid colonies (BFU-E) were predominantly My-10 positive. CFC with higher proliferative potential were more strongly My-10 positive than CFC with lower proliferative potential, and those for mixed-lineage and blast cell colonies were even more uniformly My-10 positive. Cells maintaining CFC-GM number in short-term marrow culture (pre-CFC) were found to be My-10 positive, as were lymphoid precursors defined by their content of intranuclear terminal deoxynucleotidyl transferase. More mature erythroid precursors (CFU-E) were heterogeneous for antigen expression and lost My-10 antigen progressively, in parallel with advancing maturational stage. The My-10 antigen permits rapid identification and purification of hematopoietic progenitor cells for further study or potential clinical application. The disappearance of the My-10 antigen, moreover, may be a probe for differentiation-linked cellular events.
mesh_terms: D000954:Antigens, Surface / Q000096:biosynthesis*; D001854:Bone Marrow Cells; D002469:Cell Separation; D004253:DNA Nucleotidylexotransferase / Q000032:analysis; D005312:Fetal Blood; D005434:Flow Cytometry; D006023:Glycoproteins / Q000096:biosynthesis*; D006410:Hematopoiesis; D006454:Hemoglobins / Q000032:analysis; D007962:Leukocytes / Q000166:cytology; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis*; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D006023:Glycoproteins; D006454:Hemoglobins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C050120:My-10 glycoprotein; D004253:DNA Nucleotidylexotransferase