title: Techniques for the optimum recovery of cold injured Campylobacter jejuni from milk or water.
abstract: When broth was inoculated with cells of Campylobacter jejuni that had been injured by chilling there was a fall in the viable population of up to 90%. It was greater at 43 degrees than 37 degrees C and in the presence of certain antibiotics and in some cases resulted in a surviving population that was below the minimum inoculum for subsequent growth. A technique of pre-enrichment in non-selective culture broth at 37 degrees C for 2 h before the addition of antibiotics and incubation at 43 degrees C was found to significantly reduce the fall in numbers and to improve the detection of C. jejuni in samples of raw milk and water.
mesh_terms: D000818:Animals; D002168:Campylobacter fetus / Q000254:growth & development / Q000302:isolation & purification*; D002417:Cattle; D003080:Cold Temperature; D003470:Culture Media; D005516:Food Microbiology; D005618:Fresh Water; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000382:microbiology*; D013696:Temperature; D014871:Water Microbiology
chemical_list: D003470:Culture Media