Production of dengue virus-induced macrophage cytotoxin in vivo.
We have observed that dengue virus-induced cytotoxic factor (CF) induces peritoneal and splenic macrophages in vitro to produce a cytotoxin (CF2). This study demonstrates also production of CF2 in vivo in DV-infected mice and following inoculation with CF. The cell-type responsible for CF2 production in vivo is the macrophage (M phi) as M phi-depleted mice failed to produce CF2. CF2 activity could not be observed in the serum or peritoneal fluid though it is produced in peritoneal M phi. Once stimulated, CF2 is present for 4 h in M phi. M phi can be restimulated to produce CF2 only after a refractory period of 48 h.
D000818:Animals; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003603:Cytotoxins / Q000378:metabolism* / Q000494:pharmacology*; D003715:Dengue / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000473:pathology; D011506:Proteins; D013154:Spleen / Q000166:cytology; D013997:Time Factors
D003603:Cytotoxins; D011506:Proteins