title: Complete cDNA-derived amino acid sequence of rat brown fat uncoupling protein.
abstract: Cloned cDNAs corresponding to the mitochondrial uncoupling protein of rat brown adipose tissue have been sequenced and the complete amino acid sequence of this unique membranous component is given. The N-terminal sequence of this protein is almost identical to the 14-residue N-terminal sequence previously determined by others for the hamster uncoupling protein. The uncoupling protein has no N-terminal signal extension. We found a significant sequence homology between the uncoupling protein and the ADP/ATP carrier and propose that the nucleotide binding site of the uncoupling protein is localized at the C-terminal end.
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D007425:Intracellular Membranes / Q000378:metabolism; D007473:Ion Channels; D008565:Membrane Proteins / Q000032:analysis*; D008928:Mitochondria / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics; D024101:Mitochondrial Proteins; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000071256:Uncoupling Protein 1; D004247:DNA; D000226:Mitochondrial ADP, ATP Translocases