Oral immunization with a synthetic peptide of cholera toxin B subunit. Obtention of neutralizing antibodies.
The ability of free synthetic fragments of the cholera toxin (CT), administered by parenteral or oral route, without adjuvant, to induce antibodies cross-reacting with CT was tested. Two peptides corresponding to the sequences 30-50 and 50-75 of the CT beta chain were selected and synthesized. Both free peptides, given intraperitoneally or orally, without adjuvant, elicited seric antibodies cross-reacting with CT. The anti-(P50-75) antibodies were able to neutralize the CT activity. Our results show that protection against a toxin at the systemic level can be obtained with a synthetic peptide even when administered by an oral route.
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation; D001427:Bacterial Toxins / Q000276:immunology; D002199:Capillary Permeability; D002772:Cholera Toxin / Q000276:immunology*; D003429:Cross Reactions; D004768:Enterotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D029968:Escherichia coli Proteins; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology*; D011817:Rabbits
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli