Primary structure of sperm whale luteinizing hormone.
Luteinizing hormone was extracted from sperm whale pituitaries and separated into alpha- and beta-subunits. These subunits were cleaved with cyanogen bromide, and digested with trypsin and chymotrypsin. The fragments obtained were separated and purified by gel filtration on Sephadex and by ion exchange chromatography, reversed phase chromatography and chromatoelectrophoresis. The amino acid sequence of peptides obtained was studied by dansyl-Edman's method and Edman's modification of Chang et al. The study made it possible to establish the complete amino acid sequence of sperm whale LH alpha- and beta-subunits.
D000595:Amino Acid Sequence; D000818:Animals; D002591:Cetacea; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D007986:Luteinizing Hormone / Q000302:isolation & purification*; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D010902:Pituitary Gland / Q000032:analysis*; D014357:Trypsin; D014907:Whales
D046911:Macromolecular Substances; D010446:Peptide Fragments; D007986:Luteinizing Hormone; D002918:Chymotrypsin; D014357:Trypsin; D003488:Cyanogen Bromide