Rapid identification of proteins on flat surfaces, using antibody-coated metal oxide suspensions.
Suspensions of Fe3O4 (black), Fe2O3 (red), and Cr2O3 (green) were exposed to solutions of protein A, and then each to a different antiserum to one of the following human proteins: fibrinogen, high molecular weight kininogen (HMK), albumin or immunoglobulins (IgG). Test surfaces were patterns of human proteins adsorbed out of solutions or out of plasma, onto glass as well as onto polyvinylchloride slides. They were exposed to single or mixed suspensions of the treated oxides for about 30 s and rinsed. Adhesion of each oxide onto each matching protein of these patterned test surfaces resulted, thus identifying each protein by color.
D000906:Antibodies / Q000276:immunology*; D002857:Chromium; D017608:Chromium Compounds; D005290:Ferric Compounds; D052203:Ferrosoferric Oxide; D006801:Humans; D007501:Iron; D010087:Oxides; D011506:Proteins / Q000032:analysis*; D013205:Staphylococcal Protein A
D000906:Antibodies; D017608:Chromium Compounds; D005290:Ferric Compounds; D010087:Oxides; D011506:Proteins; D013205:Staphylococcal Protein A; D002857:Chromium; C000499:ferric oxide; D007501:Iron; C023600:chromic oxide; D052203:Ferrosoferric Oxide