A method for determining ploidy distributions in liver tissue by stereological analysis of nuclear size calibrated by flow cytometric DNA analysis.
A method is presented for determining ploidy distributions in mouse liver from image analysis with stereological estimations of nuclear size in tissue sections. Nuclear profile distributions obtained from profile measurements were subjected to a mathematical unfolding procedure in order to obtain the nuclear size distributions. Based on the assumption that nuclear size increases monotonically with nuclear DNA content, flow cytometric DNA analysis of suspensions of liver cell nuclei was used to calibrate the method, thus yielding the mean nuclear size of each ploidy class, i.e., diploid, tetraploid, and octaploid nuclei. After the size interval for each of the ploidy classes was determined, the method allowed determination of ploidy distributions in mouse liver by stereological image analysis alone. The method was established from combined stereological and flow cytometric measurements on liver tissue representing two different stages of liver regeneration after two-thirds partial hepatectomy, and it was tested against an independent set of data representing a marked increase in the portion of S-phase cells.
D000818:Animals; D001699:Biometry; D002138:Calibration; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D008099:Liver / Q000032:analysis / Q000166:cytology*; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011003:Ploidies
D004247:DNA