Evasion of macrophage microbicidal mechanisms by mature sporozoites of Plasmodium yoelii yoelii.
Sporozoites of Plasmodium yoelii yoelii were incubated for 40 min with BALB/c peritoneal macrophages in the presence of nitro-blue tetrazolium (NBT). While immature oocyst sporozoites triggered the macrophage respiratory burst, as visualized microscopically by the localized reduction of NBT to insoluble formazan, 97.6% of mature salivary gland sporozoites did not induce such a response. The macrophage oxidative response was also induced by 82.7% of heat-inactivated and 95.7% of trypsin-treated salivary gland sporozoites. The relationship of these results to the infectivity and immunogenicity of malarial sporozoites is discussed.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008264:Macrophages / Q000378:metabolism* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010961:Plasmodium / Q000254:growth & development / Q000502:physiology*
D009580:Nitroblue Tetrazolium