title: [Evaluation of conformational changes in the supercoiled DNA of eukaryotic cells by direct nucleoid fluorimetry. I. The comparative information value of various methods of studying structural disorders in the supercoiled DNA in thymocytes and macrophages].
abstract: A direct fluorimetric method is elaborated for studying conformational distortions in the scDNA of "nucleoids" of macrophages and thymocytes. The method is more advantageous compared to methods of viscosimetry and sedimentation.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure*; D004278:DNA, Superhelical / Q000648:ultrastructure*; D004305:Dose-Response Relationship, Drug; D004996:Ethidium / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D009690:Nucleic Acid Conformation / Q000187:drug effects; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods; D013950:Thymus Gland / Q000187:drug effects / Q000648:ultrastructure*; D014461:Ultracentrifugation / Q000379:methods; D014783:Viscosity
chemical_list: D004278:DNA, Superhelical; D004996:Ethidium
vernacular_title: Otsenka konformatsionnykh izmeneniĭ superspiral'noĭ DNK éukarioticheskikh kletok metodom priamoĭ fluorimetrii "nukleoidov". I. Sravnitel'naia informativnost' razlichnykh metodov izucheniia narusheniĭ struktury superspiral'noĭ DNK v timotsitakh i makrofagakh.