Realtime acquisition, storage, and display of correlated three-parameter flow cytometric data.
A microprocessor-based system which performs realtime correlated acquisition, storage and display of multiparameter (3-parameter) data from a flow cytometer (FACS-III) is presented. List-mode techniques are not employed. The 3-parameter data is collected and correlated, then displayed along with cell-frequency as a realtime 3-parameter colour scattergram, while the experiment is in progress; in addition, correlated and uncorrelated higher-resolution projections of the 3-parameter data are collected and stored. The data projections may also be displayed: as 1-parameter histograms, or as 2-parameter colour or grey-scale scattergrams. Examples of 2- and 3-parameter colour scattergrams are presented. The speed and some characteristics of the realtime acquisition and display software are examined; methods to increase the realtime speed are discussed.
D001792:Blood Platelets / Q000032:analysis; D003201:Computers; D003626:Data Display; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006098:Granulocytes / Q000032:analysis; D006801:Humans; D008838:Microcomputers; D012984:Software / Q000379:methods; D013997:Time Factors