Simultaneous separate detection of low angle and large angle light scattering in an arc lamp-based flow cytometer.
A device is described for simultaneous separate detection of the light scattering of cells at low and large scattering angles in an arc lamp-based flow cytometer with epi-illumination through an oil immersion microscope objective. Light scattering was measured in a dark field configuration that allows separate detection of light scattering greater than 2 degrees and 15 degrees, respectively. Dual parameter light scattering histograms of a blood cell suspension containing various types of leukocytes were closely similar to that obtained with a commercial laser-based instrument with light scattering detection at forward and right angles. The sensitivity of the device was sufficient to measure polystyrene particles with 0.25-micron diameter. A potential application may be differentiation of bacteria.
D000818:Animals; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D007962:Leukocytes / Q000145:classification; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods