High-performance liquid chromatographic fractionation and partial characterization of cystic fibrosis serum ultrafiltrates.
Analytical separation of serum ultrafiltrates by high-performance liquid chromatography produces a distinctive peak with a retention time of 18.5-21 min (subfraction 18.5) from cystic fibrosis serum ultrafiltrates and obligate heterozygote serum ultrafiltrates, but not in significant concentrations from control or asthmatic serum ultrafiltrates. Semipreparative separation of control serum ultrafiltrates produced a small peak with similar retention time that was approximately 1% of the arbitrary absorbance units found in this cystic fibrosis subfraction. Subfraction 18.5 had biological activity only when separated from cystic fibrosis serum ultrafiltrate, but did not contain measurable amounts of C3a des-arginine and C4a des-arginine. Subfraction 18.5 is a low-molecular-weight material (1000-1400 daltons) that contains 14.9 micrograms orcinol positive material per 50 micrograms protein. The spectrum of subfraction 18.5 indicates that it has to be purified to homogeneity.
D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003550:Cystic Fibrosis / Q000097:blood*; D006801:Humans; D008970:Molecular Weight; D011188:Potassium / Q000032:analysis; D011506:Proteins / Q000032:analysis; D013363:Submandibular Gland / Q000032:analysis; D014462:Ultrafiltration
D002241:Carbohydrates; D011506:Proteins; D011188:Potassium