The complete cDNA and amino acid sequence of human apolipoprotein B-100.
We have determined the complete sequence of apolipoprotein (apo) B-100 cDNA. It is 14.1 kilobases in length and codes for a 4563-amino acid protein, including a 27-amino acid signal peptide and a 4536-amino acid mature protein. Further, we identified 2366 residues of apoB-100 by direct sequence analysis of apoB-100 tryptic peptides. The mature peptide is characterized by high hydrophobicity (0.916 kcal/residue) and predicted beta-sheet content (21%). Dot matrix analysis revealed the presence of many long internal repeats in apoB-100. The mature peptide contains 25 cysteine residues, 12 of which are in the N-terminal 500 residues. Twenty potential N-linked glycosylation sites were identified, of which 13 were proven to be glycosylated, and 4 were found not to be glycosylated by direct analysis of tryptic peptides. Our findings on apoB structure provide a basis for future experimentation on the role of apoB-100-containing lipoproteins in atherosclerosis.
D000595:Amino Acid Sequence; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D002241:Carbohydrates / Q000032:analysis; D004247:DNA / Q000032:analysis*; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood; D010446:Peptide Fragments / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D014357:Trypsin / Q000378:metabolism
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D002241:Carbohydrates; D010446:Peptide Fragments; D004247:DNA; D014357:Trypsin