Preparation and purification of the proteinase inhibitor, leupeptin, from culture filtrates of Streptomyces lavendulae.
Leupeptin (N-acetyl-L-leucyl-L-leucyl-L-argininal) was produced as an extracellular metabolite by Streptomyces lavendulae. Addition of lysine to the growth medium stimulated leupeptin production from 200 to 1400 micrograms/ml. Incubation of late exponential phase mycelium in a synthetic medium was used to prepare single labelled (3H) and double labelled (3H/14C) inhibitor for metabolic studies. An improved purification scheme that generates leupeptin of high purity with good recoveries is also reported.
D002250:Carbon Radioisotopes; D003470:Culture Media; D007700:Kinetics; D007976:Leupeptins / Q000096:biosynthesis / Q000302:isolation & purification*; D009842:Oligopeptides / Q000302:isolation & purification*; D013302:Streptomyces / Q000254:growth & development*; D014316:Tritium
D002250:Carbon Radioisotopes; D003470:Culture Media; D007976:Leupeptins; D009842:Oligopeptides; D014316:Tritium; C032854:leupeptin