Rapid sample preparation method for the determination of chloramphenicol in swine muscle by high-performance liquid chromatography.
A simple and rapid sample preparation method for the determination of chloramphenicol in swine muscle tissue at the 10 micrograms/kg level is described. The method comprises sonication-aided extraction with ethyl acetate, addition of hexane to the extract and cleaning up and concentration of the extract on a small column packed with silica gel. Analysis was performed by high-performance liquid chromatography on a ChromSep column with ChromSpher C8 using acetonitrile-sodium acetate buffer as the mobile phase. Detection was performed at 280 nm. Mean recoveries from spiked muscle samples were 79 +/- 3% (10-50 micrograms/kg). The distribution of chloramphenicol in different muscle and fatty tissues from a pig to which a single dose of chloramphenicol was administered was also investigated.
D000273:Adipose Tissue / Q000032:analysis; D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009132:Muscles / Q000032:analysis*; D013552:Swine
D007202:Indicators and Reagents; D002701:Chloramphenicol