title: Economical modification of a radioimmunoassay of digoxin with negligible interference from cord and neonatal sera.
abstract: The Amerlex digoxin radioimmunoassay was modified to reduce the running cost by five times. The modified method compared well with the original method (y = 1.0138x + 0.00916, r = 0.9936). Using the modified method, the performance with the American Association for Clinical Chemistry quality control program was consistently satisfactory for 1 year. When normal cord and neonatal sera were tested with the modified method, 41% of the 22 samples of cord serum and 68% of the 19 samples of neonatal serum produced digoxin levels less than or equal to 0.05 ng/ml. The highest digoxin level produced by cord and neonatal sera was 0.3 ng/ml.
mesh_terms: D004077:Digoxin / Q000097:blood*; D005312:Fetal Blood / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D011863:Radioimmunoassay / Q000191:economics / Q000379:methods*
chemical_list: D004077:Digoxin