Protein kinase C translocation in relation to proliferative state of C3H 10T1/2 cells.
Protein kinase C (PKC) activity of cytosol and membrane fractions of 10T1/2 cells was studied. In cytosol of fast growing cells PKC activity was found in material eluted with 0.150 M NaCl whereas in membrane fractions activity was eluted with 0.065 and 0.150 M NaCl. In the membrane fraction of confluent cells, in contrast to cytosol, very low PKC activity was observed. The translocation pattern of PKC activity eluted with 0.065 M NaCl may be associated with proliferation.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology; D051379:Mice; D008809:Mice, Inbred C3H; D009994:Osmolar Concentration; D011493:Protein Kinase C / Q000378:metabolism*; D012965:Sodium Chloride
D012965:Sodium Chloride; D011493:Protein Kinase C