[A method of determining 2-oxoglutarate dehydrogenase activity in intact mitochondria].
A rather simple method is suggested for measuring the activity of 2-oxoglutarate dehydrogenase of intact mitochondria. The method is based on the determination of the rate of exogenic 2-oxoglutarate decrease in the mitochondrial suspension. Experiments with sodium arsenite and comparison of kinetic parameters of the 2-oxoglutarate, dehydrogenase reaction and transport of 2-oxoglutarate to mitochondria have shown that the measurable exogenic 2-oxoglutarate oxidation rate corresponds to the 2-oxoglutarate dehydrogenase activity in intact mitochondria. The method made it possible to establish the stimulating effect of ADP on the 2-oxoglutarate dehydrogenase activity of intact mitochondria and the absence of such an effect in destructed mitochondria.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D005260:Female; D007655:Ketoglutarate Dehydrogenase Complex / Q000032:analysis*; D007656:Ketoglutaric Acids / Q000378:metabolism; D007658:Ketone Oxidoreductases / Q000032:analysis*; D008297:Male; D008722:Methods; D008930:Mitochondria, Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D017670:Sodium Compounds
D018053:Arsenites; D007656:Ketoglutaric Acids; D017670:Sodium Compounds; C017947:sodium arsenite; D000244:Adenosine Diphosphate; D007658:Ketone Oxidoreductases; D007655:Ketoglutarate Dehydrogenase Complex; D001151:Arsenic
Metod izmereniia 2-oksoglutaratdegidrogenaznoĭ aktivnosti intaktnykh mitokhondriĭ.