Identification and characterization of L-triiodothyronine receptors in cells of glial and neuronal origin.
High affinity-low capacity binding sites for thyroid hormone have been identified in the nuclei of glial (C6) and neuronal (Neuro 2A) cultured cells. Equilibrium dissociation constants, determined by Scatchard analysis, were very similar in both types of cells (0.2-0.3 nM). The relative affinity of hormonal analogs was also similar: the affinity for T3 was lower than for triiodothyroacetic acid and higher than for T4 or tetraiodothyroacetic acid. The sedimentation coefficients obtained by gradient centrifugation of nuclear receptor extracted with 0.4 M KCl or excised by micrococcal nuclease digestion were 3.5 S and 6.5 S, respectively. These results suggest that the thyroid hormone receptor is not restricted to neuronal cells, but also appears in cells of glial origin.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D005910:Glioma / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D013974:Thyroxine / Q000031:analogs & derivatives / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism
D002087:Butyrates; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D020148:Butyric Acid; C011126:tetraiodothyroacetic acid; D013974:Thyroxine