Investigation of the chemiluminescence response of human neutrophils and mononuclear cells.
The activation of chemiluminescence (CL) of human phagocytes was investigated by the use of the amplifiers, luminol and lucigenin, in parallel studies. The optimal doses for opsonized zymosan, WGA and HAIG were determined, and the integral CL of polymorphonuclear and mononuclear cells from healthy probands and patients with enzyme defects related to the cellular O2 metabolism were compared. Both cell types showed striking differences with respect to the kinetics and integral responses and in dependence on the amplifier after stimulation with the various activators. These results suggest that there is no intimate relationship between O2-release and production of the luminol reactive oxygen species.
D000166:Acridines; D002374:Catalase / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G; D007700:Kinetics; D037102:Lectins; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology; D014909:Wheat Germ Agglutinins; D015054:Zymosan
D000166:Acridines; D007074:Immunoglobulin G; D037102:Lectins; D014909:Wheat Germ Agglutinins; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D006632:Histamine; D015054:Zymosan; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen