Distribution of choline acetyltransferase activity in rat spinal cord--influence of primary afferents?
Choline acetyltransferase (CAT) activity was measured in various regions of rat spinal cord. In the ventral cord, enzyme activity was 2 to 3 times higher than in dorsal cord. In dorsal spinal cord, there was a gradient in enzyme activity, increasing CAT activity being observed in more caudal segments. In autonomic regions intermediate levels were measured. Bilateral transection of the sciatic nerve reduced CAT activity in the ventral horn of lumbar spinal cord, whereas CAT activity in the dorsal horn remained unchanged. Capsaicin pretreatment had no effect on CAT activity in any spinal cord region. Although a similar distribution of cholinergic neurones and primary afferent endings in rat dorsal spinal cord was described, no conclusive statement as to a possible functional interaction can be given.
D000344:Afferent Pathways / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D009475:Neurons, Afferent / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
D002795:Choline O-Acetyltransferase; D002211:Capsaicin