The binding of skeletal muscle C-protein to regulated actin.
The binding of C-protein, a component of thick filament of myofibrils, to regulated actin filaments in the presence or absence of Ca2+ was studied. The amount of C-protein bound to regulated actin filaments in the presence of Ca2+ was higher than those in the absence of Ca2+. The addition of C-protein to regulated actin caused an increase in turbidity, especially in the presence of Ca2+, and this was found to result from side-by-side association of actin filaments into bundles. In the absence of Ca2+, no actin filament bundles were formed.
D000199:Actins / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000502:physiology; D002352:Carrier Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000378:metabolism*; D011485:Protein Binding; D000099163:Myosin Binding Protein C
D000199:Actins; D002118:Calcium; D002352:Carrier Proteins; D009124:Muscle Proteins; D000099163:Myosin Binding Protein C