Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.
The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5' terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.
D001710:Biotin / Q000032:analysis; D005456:Fluorescent Dyes; D007553:Isotope Labeling / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis
D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D001710:Biotin