title: Vasopressin binds to microvessels from rat hippocampus.
abstract: Recent evidence suggests that vasopressin may influence the permeability of the endothelium of brain capillaries. We measured the binding of [125I]arginine-8-vasopressin ([125I]AVP) to microvessels isolated from different regions of the rat brain. The study revealed saturable and specific binding of [125I]AVP to microvessels isolated from hippocampus. Scatchard analysis confirmed a single class of high affinity sites with an equilibrium dissociation constant, Kd, of 3.2 nM and an apparent maximal binding capacity of 205 fmol/mg protein. No binding was observed to microvessels from neocortex and striatum.
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D006624:Hippocampus / Q000098:blood supply*; D007700:Kinetics; D008833:Microcirculation / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001127:Arginine Vasopressin