Binding of recA protein from E. coli to double-stranded DNA: influence of the degree of superhelicity.
The binding of the recA protein from E. coli to supercoiled double-stranded DNA is strongly dependent upon the superhelical density of the DNA molecule. A threshold of superhelical density is required for strong binding in the presence of ATP. This finding is consistent with a model in which recA protein first binds to unpaired regions and then polymerises on the contiguous double-stranded lattice.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D004278:DNA, Superhelical / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011938:Rec A Recombinases / Q000378:metabolism*; D013873:Thionucleotides / Q000494:pharmacology
D004278:DNA, Superhelical; D013873:Thionucleotides; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D011938:Rec A Recombinases