Identification of calmodulin in new-born rat calvaria.
The extract of new-born rat calvaria was chromatographed on DEAE-cellulose. Calmodulin activity was eluted at 0.3 and 0.4 M NaCl and markedly inhibited by trifluoperazine and W-7, calmodulin antagonists. The fractions with calmodulin activity contained a protein the electrophoretic migration of which on sodium dodecyl sulfate-polyacrylamide gel was accelerated by Ca2+. Its apparent molecular weight was about 18 or 20K in the presence or absence of Ca2+, respectively. With 45Ca-autoradiography, the protein was shown to have a high affinity for Ca2+. Thus calmodulin could be readily identified in new-born rat calvaria.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001345:Autoradiography; D002147:Calmodulin / Q000032:analysis* / Q000037:antagonists & inhibitors; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000032:analysis; D051381:Rats; D012886:Skull / Q000032:analysis*; D013449:Sulfonamides / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology
D002147:Calmodulin; D011506:Proteins; D013449:Sulfonamides; D014268:Trifluoperazine; C017967:W 7