Simultaneous quantitation of 1,8,9-anthracenetriol, 1,8-dihydroxy-9,10-anthraquinone, and 1,8,1',8'-tetrahydroxy-10,10'-dianthrone by reversed-phase high-performance liquid chromatography.
A sensitive and specific reversed-phase high-performance liquid chromatographic method was developed for the simultaneous analysis of anthralin (1,8,9-anthracenetriol) and its known degradation products: quinone (1,8-dihydroxy-9,10-anthraquinone) and dimer (1,8,1',8'-tetrahydroxy-10,10'-dianthrone). The method provides for rapid and simple quantitation of nanogram amounts of the three compounds. The molar absorptivities for anthralin in different solvents and solvent systems, including the mobile phase, are presented.
D000873:Anthracenes / Q000032:analysis*; D000875:Anthralin / Q000032:analysis*; D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D011809:Quinones / Q000032:analysis; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000873:Anthracenes; D000880:Anthraquinones; D007202:Indicators and Reagents; D011809:Quinones; D012997:Solvents; C044840:1,8,1',8'-tetrahydroxybisanthrone; D000875:Anthralin