title: Isolation and labelling of human leukocytes with 99mTc.
abstract: The role of the leukocyte isolation procedure on cell labelling with 99mTc has been evaluated. Separation of leukocytes was performed by two procedures: sedimentation on methyl cellulose, followed by discontinuous gradient centrifugation; methyl cellulose sedimentation and hypotonic haemolysis of residual red blood cells. After washing the cells in saline and incubation with a stannous pyrophosphate agent, the leukocytes were labelled with 5-10 mCi 99mTc. Procedure 1 gave a higher purity but lower recovery of polymorphonuclear leukocytes, and a minor contamination of red blood cells. 99mTc labelling of cells was slightly more efficient with this method, probably due to the presence of red blood cells. Procedure 1 is recommended for in vitro studies on cell kinetics and procedure 2 is recommended for clinical use.
mesh_terms: D002469:Cell Separation / Q000379:methods; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D009504:Neutrophils; D013667:Technetium
chemical_list: D013667:Technetium