A simplified method for metabolic studies in conscious swine.
Reliable short-term blood access in conscious swine was provided by implanting multiple silastic catheters. Catheters were inserted into the aorta, hepatic vein, portal vein, and inferior vena cava through a midline laparotomy incision. Multiple catheters also were placed into the external jugular vein through a separate cervical incision. Catheter patency rates for blood withdrawal on the sixth post-operative day were: arterial 100%, hepatic 91%, portal 86%, inferior vena cava 71%. No animal had major wound or catheter infection on the seventh post-operative day. The methods described allow metabolic studies, including measurements of splanchnic blood flow, to be carried out either acutely or for up to at least 7 days post-operatively.
D000818:Animals; D002404:Catheterization / Q000379:methods / Q000662:veterinary*; D002408:Catheters, Indwelling / Q000662:veterinary; D005260:Female; D012826:Silicone Elastomers; D013552:Swine / Q000097:blood* / Q000378:metabolism
D012826:Silicone Elastomers