Biological and growth properties of a 20-methyl-cholanthrene transformed murine fibroblast cell line (PMM-14) established from primary culture.
Some major transformation related biological and growth properties have been analysed in an in vitro, system to characterize the PMM-14 cell line, a 20-methylcholanthrene induced transformed mouse embryonic fibroblast cell line. The population doubling time of this cell line was 19 h with moderately high saturation density and plating efficiency. Attachment detachment properties showed reduced adhesion to substratum. Cytogenetic study revealed the existence of a large number of Robertsonian biarmed chromosomes with hypertriploid modal range (60-69) represented by about 21% cells. These cells showed enhanced concanavalin A agglutinability which was reduced by trypsin treatment. Its neoplastic nature was finally established by its ability to grow in agar with a high plating efficiency.
D000362:Agar; D000818:Animals; D002448:Cell Adhesion; D002449:Cell Aggregation; D002453:Cell Cycle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D003208:Concanavalin A; D003470:Culture Media; D005347:Fibroblasts; D066298:In Vitro Techniques; D007621:Karyotyping; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D014357:Trypsin / Q000378:metabolism
D003470:Culture Media; D003208:Concanavalin A; D008748:Methylcholanthrene; D000362:Agar; D014357:Trypsin