Immunohistochemical evidence of the origin of human corneal endothelial cells and keratocytes.
Human corneal endothelial cells and keratocytes were immunohistochemically examined using antisera against neuronal tissue antigens, namely, S-100 protein and neuron-specific enolase (NSE), and intermediate filaments such as vimentin, neurofilament, and glial fibrillary acidic protein (GFAP). The corneal endothelium and keratocytes showed a positive immunoreaction to neuron-specific enolase and S-100 protein antisera, and these cells were also stained with antiserum against vimentin, a main intermediate filament of mesenchymal cells. These immunohistochemical findings provide additional evidence that the corneal endothelium and keratocytes originate from neural crest cells and then differentiate into mesenchymal cells.
D003315:Cornea / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D004727:Endothelium / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D006651:Histocytochemistry; D006801:Humans; D007120:Immunochemistry; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D009418:S100 Proteins; D014746:Vimentin; D010751:Phosphopyruvate Hydratase