[Conditions for forming ascitic tumors in hybridoma cultivation in vivo].
The conditions of the formation of ascitic cells in BALB/c mice injected with hybridoma cells were studied. All the hybridomas under study, producing monoclonal antibodies to viral antigens, induced the formation of ascitic tumors when introduced into the abdominal cavity of BALB/c mice pretreated with sensitizing agents. In the mice pretreated with pristane hybridoma cells took at a rate of 43-80% and in the mice pretreated with Freund's complete adjuvant, 31-70%. Angara oil and perfume oil, as well as Bayol F, were less effective. The time of the formation of ascites was inversely proportional to the dose of the injected cells, while the volume of ascitic fluid depended rather on the type of hybridoma and not on the dose of the injected cells. The study showed that the use of physiological saline or culture medium without serum for washing the abdominal cavity of mice after withdrawing ascites permitted the additional collection of 2.6-13.7 million hybrid cells, as well as a considerable amount of immunoglobulins.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D001201:Ascites / Q000209:etiology* / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006825:Hybridomas / Q000276:immunology* / Q000637:transplantation; D007114:Immunization / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000209:etiology* / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal
Izuchenie usloviĭ obrazovaniia astsitnykh opukholeĭ pri kul'tivirovanii gibridom in vivo.