In vitro transformation of C3H/10T1/2 and NIH/3T3 cells by acrylonitrile and acrylamide.
Acrylonitrile (AN) and acrylamide (AM) are carcinogenic in a number of rodent organs and AN is a suspected human carcinogen. We sought to determine whether AN and/or AM could produce morphological transformation in vitro in C3H/10T1/2 and NIH/3T3 mouse fibroblast cells. Both AN and AM induced a dose-dependent cytotoxic effect in C3H/10T1/2 and NIH/3T3 cells and readily transformed both cell lines. Our conclusions are based on the appearance of cells exhibiting a transformed phenotype and growth in soft agar. AN and AM transformed NIH/3T3 cells to a greater extent than C3H/10T1/2 cells. This is the first reported transformation of cells in vitro by AM.
D020106:Acrylamide; D000178:Acrylamides / Q000633:toxicity*; D000181:Acrylonitrile / Q000378:metabolism / Q000633:toxicity*; D000362:Agar; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000473:pathology; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D051379:Mice; D009570:Nitriles / Q000633:toxicity*
D000178:Acrylamides; D009570:Nitriles; D020106:Acrylamide; D000362:Agar; D004247:DNA; D000181:Acrylonitrile