Chemical synthesis of octadeoxyribonucleotides containing sequence-specific 3'-deoxy-3'-chlorouridine residues and 2'-5' internucleotide linkages by the phosphite chloridite approach.
3'-Deoxy-3'-chlorouridine has been chemically incorporated into two octanucleotides at various positions using in situ prepared phosphite chloridite intermediates. The behaviour of these modified selfcomplementary DNA fragments containing 2'-5' internucleotide bonds in the neighbourhood of 3'-deoxy-3'-chlororibose in respect to chemical sequencing, polynucleotide kinase and T4 DNA ligase has been studied.
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003857:Deoxyuridine / Q000031:analogs & derivatives; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; C049885:3'-chloro-3'-deoxyuridine; D003857:Deoxyuridine