Selective 1H-NMR relaxation investigations of membrane-bound drugs in vitro. 2. Angiotensin II.
Binding interactions between human angiotensin II and dipalmitoylphosphatidylcholine bilayer vesicles have been detected by measuring the selective proton spin-lattice relaxation rates of aromatic protons within the peptide. Involvement of the imidazole moiety of the His-6 residue has been demonstrated by the pH dependence of the NMR observables. A lower limit of the binding constant has been evaluated at 78.12 mol-1 dm3 for the interaction involving nonionic intermolecular forces between aromatic residues and the lipid matrix.
D000804:Angiotensin II; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008954:Models, Biological; D011663:Pulmonary Surfactants; D013816:Thermodynamics
D008051:Lipid Bilayers; D011663:Pulmonary Surfactants; D000804:Angiotensin II