title: Growth of mixed erythroid-granulocytic colonies in culture derived from bone marrow of patients with paroxysmal nocturnal hemoglobinuria without addition of exogenous stimulator.
abstract: Hematopoietic progenitor cell cultures were studied in 16 patients with paroxysmal nocturnal hemoglobinuria (PNH). Both erythroid and granulocyte-macrophage progenitors in the blood and bone marrow decreased despite hypercellular marrow. In eight out of 16 patients, mixed erythroid-granulocytic colonies were observed in the bone marrow cultures without erythropoietin and colony-stimulating factor. Removal of monocytes and T-lymphocytes before culture failed to abrogate spontaneous mixed-colony formation. Conditioned media prepared from blood and bone marrow of PNH patients could not promote the growth of mixed colonies in normal individuals. The autonomous, spontaneous mixed-colony formation in patients with PNH is an abnormality of PNH stem cells not described previously.
mesh_terms: D000917:Antibody Formation; D001854:Bone Marrow Cells; D002450:Cell Communication; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004920:Erythropoiesis; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006457:Hemoglobinuria, Paroxysmal / Q000473:pathology*; D006801:Humans; D008297:Male
chemical_list: D003470:Culture Media