[DNA-phospholipid interactions. A study using lipid-specific fluorescent and photoreactive probes].
The interaction of phospholipids with phage T7 DNA was investigated using anthryl-vinyl-labeled and photoactivable phosphatidylcholine and sphingomyelin. Fluorescence polarization studies demonstrated that, in the presence of DNA, the fluorophore mobility is diminished as its distance from the polar head-group is increased. Immobilization of lipid chains is enhanced by Ca2+ ions, the effect being more pronounced for sphingomyelin than for phosphatidylcholine derivatives. On the other hand, phospholipids with a photoactivable group could not be crosslinked to DNA in the DNA-phospholipid complexes, evidencing against the presence of contacts between lipids and DNA.
D000818:Animals; D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D005456:Fluorescent Dyes; D007739:L Cells; D051379:Mice; D010743:Phospholipids / Q000032:analysis*; D013604:T-Phages / Q000032:analysis
D004279:DNA, Viral; D005456:Fluorescent Dyes; D010743:Phospholipids; D004247:DNA
DNK-fosfolipidnoe vzaimodeĭstvie. Issledovanie s pomoshch'iu lipidspetsificheskikh fluorestsentnykh i fotoreaktivnykh zondov.