A modified ferrozine method for the measurement of enzyme-bound iron.
A general procedure for the determination of the iron content of enzymes by digestion with methanesulfonic acid to release protein-bound iron has been developed. This procedure replaces the tedious and potentially hazardous method of wet ashing with concentrated nitric-sulfuric-perchloric acids. The method has been used to determine the stoichiometry of iron for nanomole quantities of heme-iron proteins, iron-sulfur proteins, complex iron-sulfur proteins, as well as in phenylalanine hydroxylase, an enzyme with iron in an undetermined coordination.
D002374:Catalase / Q000032:analysis; D004798:Enzymes / Q000032:analysis*; D005297:Ferrozine; D007202:Indicators and Reagents; D007501:Iron / Q000032:analysis*; D010651:Phenylalanine Hydroxylase / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods
D004798:Enzymes; D007202:Indicators and Reagents; D005297:Ferrozine; D007501:Iron; D002374:Catalase; D010651:Phenylalanine Hydroxylase