A new covalently modified support for gas-liquid phase sequencing.
A new method for activation of glass fiber supports for immobilisation of proteins and peptides in gas-liquid phase sequencing is described. The new support offers several advantages over the presently used carrier polybrene: no precycling is required, initial yields are improved and background contamination is lower. This leads to an overall increase in detection sensitivity. The derivatisation method includes acid activation and subsequent covalent coating of glass fibers with quaternary ammonium groups thereby giving the glass surface a high binding capacity for both proteins and peptides. The activated glass has been successfully used for sequencing proteins and peptides isolated by HPLC as well as by electroelution from polyacrylamide gels.
D002849:Chromatography, Gas / Q000379:methods*; D005898:Glass / Q000592:standards; D006583:Hexadimethrine Bromide / Q000494:pharmacology; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012821:Silanes / Q000494:pharmacology*; D012825:Silicon / Q000494:pharmacology*; D014297:Trimethylsilyl Compounds / Q000494:pharmacology*
D010455:Peptides; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D012821:Silanes; D014297:Trimethylsilyl Compounds; C050204:trimethyl-(3-(trimethoxysilyl)propyl)ammonium; D006583:Hexadimethrine Bromide; D012825:Silicon