Monoclonal antibodies against the glycoproteins of mumps virus: fusion inhibition by anti-HN monoclonal antibody.
Six monoclonal antibodies (MAbs) against the haemagglutinin-neuraminidase (HN) protein and three against the fusion (F) protein of mumps virus were obtained. Anti-HN MAbs were classified into three antigen-specific groups in competitive binding assays. All of the anti-HN MAbs showed virus-neutralizing and haemolysis-inhibiting activities and inhibited fusion from without (FFWO). Three of them inhibited fusion from within (FFWI) and the cell-to-cell spread of infection. The most effective MAb in inhibiting FFWI possessed neuraminidase-inhibiting activity and little haemagglutination-inhibiting activity. All anti-F MAbs inhibited virus-induced haemolysis but none of them inhibited FFWO, FFWI or the cell-to-cell spread of infection.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D002459:Cell Fusion; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D015707:HN Protein; D009109:Mumps virus / Q000276:immunology / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000502:physiology; D014760:Viral Fusion Proteins / Q000276:immunology* / Q000502:physiology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015707:HN Protein; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins