Receptors for chorionic gonadotropin in the corpus luteum of the rhesus monkey during simulated early pregnancy: lack of down-regulation.
Stimulation of the primate corpus luteum (CL) by endogenous CG in early pregnancy or by exogenous hCG in simulated early pregnancy is transient, possibly due to a drop in the number of available gonadotropin receptors in the CL. The objective of the current study was to determine if this reduction in available CG receptors in the CL is due to down-regulation of the total receptor population or occupation of receptors by CG. CL were removed from rhesus monkeys (n = 27) during prolonged CG exposure in simulated early pregnancy. Luteal tissue was homogenized, and a particulate preparation (20,000 g) was formed. Bound gonadotropin was eluted from half of each tissue preparation with 0.05 M Na acetate-HCl in 0.1% gelatin (pH 3.3); the other half was treated with control buffer (0.05 M Tris-HCl, pH7.4). The hCG in the eluate was quantified by RIA and used to estimate the number of occupied receptors. Numbers and affinities of receptors were estimated by Scatchard analyses of specific [125I]hCG binding. Serum concentrations of progesterone increased within 9 h of hCG treatment and declined between 3 and 10 days of treatment. Whereas the number of available receptors declined from 0 h to 10 days of hCG treatment, the number of receptors occupied by hCG increased throughout treatment from 0.19 +/- 0.01 fmol/mg tissue (mean +/- SE) after 2 h of treatment to 7.76 +/- 1.73 fmol/mg after 10 days of treatment (P less than 0.05). The total number of receptors (available plus occupied) did not change throughout the 10-day treatment period (11.93 +/- 2.11 fmol/mg at 0 h vs. 9.64 +/- 1.86 at 10 days; P greater than 0.10). The dissociation constants (Kd) for CG binding were greater (P less than 0.05) after 6 days (4.10 +/- 0.57 X 10(-10) M) and 10 days (5.24 +/- 0.54 X 10(-10) M) of treatment than after 0-3 days (0.95 +/- 0.06 X 10(-10) M) of treatment. Notably, the percentage of total receptors able to rebind hCG after elution declined from 0 h (79.0 +/- 5.4%) to 10 days (23.0 +/- 3.2%; P less than 0.05). Rebindability was highly correlated with the number of available (r = 0.906; P less than 0.05) and occupied (r = -0.999; P less than 0.01) CG receptors. Thus, the reduction in available gonadotropin-binding sites in the CL during simulated early pregnancy is largely due to occupancy rather than down-regulation of receptors.(ABSTRACT TRUNCATED AT 400 WORDS)
D000818:Animals; D006063:Chorionic Gonadotropin / Q000378:metabolism / Q000494:pharmacology; D003338:Corpus Luteum / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D008253:Macaca mulatta; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011374:Progesterone / Q000097:blood; D011974:Receptors, LH / Q000187:drug effects / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D011974:Receptors, LH; D000431:Ethanol; D011374:Progesterone