title: Decrease in myosin light chain kinase activity of rabbit fast muscle by chronic stimulation.
abstract: Analysis of myosin light chain kinase (MLCK) activity in tibialis anterior muscles of the rabbit revealed that chronic stimulation at a frequency of 10 Hz for 24 h per day reduced the enzyme activity in a time-dependent manner. Since fast twitch muscle contains significantly more myosin light chain kinase than slow twitch muscle, the observed reductions are consistent with the type of fast-to-slow transformation observed for other type-specific muscle characteristics. The present data also indicate that the stimulation-induced decrease in MLCK activity precedes the fast-to-slow conversion of the myosin molecule as judged by pyrophosphate-polyacrylamide gel electrophoresis.
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D009119:Muscle Contraction; D009132:Muscles / Q000201:enzymology*; D009219:Myosin-Light-Chain Kinase; D009218:Myosins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000032:analysis*; D011817:Rabbits
chemical_list: D011494:Protein Kinases; D009219:Myosin-Light-Chain Kinase; D009218:Myosins