[Chromatographic behavior of the hepatitis B virus surface antigen in donor plasma].
Behavior of HBsAg in adsorption chromatography of the antigen-containing plasma in a column with unmodified macropore silica was studied. Under the employed conditions of overlaying and elution, five maximum yields of plasma proper proteins from the column (peaks 1-5) were observed. HBsAg was determined mainly in the material of peaks 4 and 5 in which the dominating host protein was lipoprotein HDL. No HBsAg was detected in albumin-enriched fraction. Similarities of chromatographic behavior of all the three classes of particles whose surface is presented by HBsAg, 20 nm particles, filamentous forms, and Dane particles, were observed.
D000327:Adsorption; D001782:Blood Donors; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D008854:Microscopy, Electron
D001798:Blood Proteins; D006514:Hepatitis B Surface Antigens
Khromatograficheskoe povedenie poverkhnostnogo antigena virusa gepatita B v donorskoĭ krovi.