Sensitivity of Deinococcus radiodurans to near-ultraviolet radiation.
Although Dienococcus radiodurans is notoriously resistant to far-ultraviolet radiation (FUV; 254 nm), it is highly sensitive to near-ultraviolet radiation (NUV; 300-400 nm), thus demonstrating that the mechanisms of damage (and/or recovery) by the two types of irradiation are different. This observed difference between FUV and NUV effects in D. radiodurans agrees with previous studies with Escherichia coli. Near-ultraviolet radiation produces DNA damage which is presumed to be single-strand breaks (SSB) in the DNA of D. radiodurans. Unique lesions, such as DNA-protein crosslinks could not be demonstrated in this study. Cells that were pre-irradiated with a small dose of NUV were subsequently protected against inactivating doses of NUV. The data presented are consistent with induced DNA repair following NUV damage in D. radiodurans; this is in contrast to FUV damage where DNA repair is constitutive but not induced.
D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000032:analysis; D008837:Micrococcus / Q000187:drug effects / Q000528:radiation effects*; D013057:Spectrum Analysis; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004268:DNA-Binding Proteins