The tissue distribution of NK-9 positive lymphoid cells including NK and AK cells and their precursors.
The distribution of NK-9 antigen presenting cells in human lymphoid tissues including spleen, tonsil, lymph node, and thymus was studied. The staining were done on formalin-fixed, histological sections. In peripheral blood NK-9 antigens are expressed on allospecific (CTL) and nonspecific (NK and AK) cytotoxic lymphocytes. In lymphoid tissues, the distribution of NK-9 positive cells was definitely different from "normal" T cell distribution, measured by pan T monoclonal markers. In tonsils and lymph nodes NK-9 positive cells were mainly located in and around germinal centers. However, only a proportion of these areas were NK-9 positive. In spleen NK-9 positive cells were located mainly in periarteriolar lymphocyte sheats and in germinal centers. Thymuses were practically NK-9 negative. These results support the earlier suggestions that cytotoxic cells take part in the immunoregulation in lymphoid tissues.
D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006651:Histocytochemistry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D014066:Palatine Tonsil / Q000166:cytology; D010209:Paper; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003101:Collodion