Leishmania braziliensis: localization of glycoproteins in promastigotes.
Two species of glycoproteins from Leishmania braziliensis promastigotes of apparent molecular weights of 53,000 (glycoprotein 53) and 47,000 (glycoprotein 47) were localized. Four lectins with different sugar specificities bound to the blotting sheet to which the electrophoretically separated materials were transferred. Concanavalin A and Ricinus communis agglutinin bound to the band of glycoprotein 53 and the lectin from Dolichos biflorus bound to the band of glycoprotein 47. Wheat germ agglutinin bound to the bands of both glycoproteins. Histochemical examinations using fluorescence labeled lectins demonstrated that the glycoproteins 53 and 47 were located on the cell surface and in the cytoplasm of promastigotes, respectively. The results are consistent with the result of agglutination test.
D000372:Agglutination Tests; D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D003208:Concanavalin A / Q000494:pharmacology; D003593:Cytoplasm / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins / Q000494:pharmacology; D007891:Leishmania / Q000032:analysis*; D007892:Leishmania braziliensis / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure; D008970:Molecular Weight; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; C017707:Ricinus communis agglutinin-1; D014909:Wheat Germ Agglutinins; C036554:dolichos biflorus agglutinin; D003208:Concanavalin A