A simple method for high-quality frozen red cells in blood group serology.
A high-glycerol method for long-term storage of red cells for use in blood group serology testing is described. Total freezing-thawing-washing hemolysis was 8.05 +/- 0.3 percent. Enzyme treatment could be performed before freezing or after reconstitution. The cells performed well with manual and automated techniques. No changes in agglutinability were noted during 10 days of liquid storage at 4 degrees C following thawing.
D001793:Blood Preservation / Q000379:methods*; D004796:Clinical Enzyme Tests; D003298:Coombs Test; D004912:Erythrocytes; D005615:Freezing; D005990:Glycerol; D006801:Humans
D005990:Glycerol