Unscheduled DNA synthesis after beta-irradiation of mouse skin in situ.
The skin of ICR mouse was irradiated with beta-rays from 90Sr-90Y with surface doses up to 30 krad. Unscheduled DNA synthesis (UDS) was measured by autoradiography after labeling the skin with radioactive thymidine using the forceps-clamping method. The level of UDS in epithelial cells of the skin was detected as an increasing function of radiation dose. Fibroblastic cells, compared with epithelial cells and hair follicle cells at the same depth of the skin, showed a lower level of UDS, indicating a lower DNA repair activity in fibroblasts. Cancer risk of the skin was discussed.
D000818:Animals; D004260:DNA Repair; D004848:Epithelium / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D011839:Radiation, Ionizing; D012867:Skin / Q000528:radiation effects*; D012879:Skin Physiological Phenomena