Anomeric preference of glucose phosphorylation and glycolysis in human erythrocytes.
Lactate output from the alpha and beta anomers of glucose was measured in intact human erythrocytes at 37 degrees C; and glucose anomer phosphorylation, in human erythrocyte homogenates. The rates of both glucose metabolism (lactate output) and phosphorylation were higher in the presence of beta-D-glucose as distinct from alpha-D-glucose at three glucose concentrations used (2, 5, and 10 mM). Thus, the v beta/v alpha ratios of metabolism and phosphorylation of glucose at 2 mM were 1.24 and 1.22, respectively. The results indicate that the beta preference of hexokinase, a rate-limiting enzyme in glycolysis, is reflected in beta-preferential glycolysis.
D001786:Blood Glucose / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004912:Erythrocytes / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D007700:Kinetics; D007773:Lactates / Q000097:blood; D010766:Phosphorylation; D013237:Stereoisomerism
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D007773:Lactates