A fluorometric high performance liquid chromatographic assay for vanillylmandelic acid (VMA) developed from a commercial kit method.
A fluorometric procedure based on the Bio-Rad Vanillylmandelic Acid (VMA) by High Performance Liquid Chromatography (HPLC) Test was developed. Detection by fluorescence provides better sensitivity and specificity than the Pisano spectrophotometric method. Use of a C18 mini-column extraction to extract VMA prior to HPLC analysis significantly reduced the late eluting peaks. A linear range was established up to 400 mumol/L of VMA. Within-run and between-run CV's were 3.0% and 3.7%, respectively. In a comparison of this fluorometric method with the Pisano spectrophotometric method, a linear regression of y = 0.807x + 2.185 was obtained with a correlation coefficient of 0.945. In the analysis of over 50 samples, no intereferences have been found.
D000310:Adrenal Gland Neoplasms / Q000175:diagnosis / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry; D006801:Humans; D009447:Neuroblastoma / Q000175:diagnosis / Q000652:urine; D010673:Pheochromocytoma / Q000175:diagnosis / Q000652:urine; D011933:Reagent Kits, Diagnostic / Q000706:statistics & numerical data; D013053:Spectrophotometry; D014642:Vanilmandelic Acid / Q000032:analysis / Q000652:urine*
D011933:Reagent Kits, Diagnostic; D014642:Vanilmandelic Acid