title: Actions of morphine and enkephalins on the internal anal sphincter of the cat: relevance for the physiological role of opiates.
abstract: The effects of Leu-enkephalin, Met-enkephalin and morphine on the electrical activity of the internal anal sphincter were studied in anesthetized spinalized cats and in vitro on sphincteric muscle strips. All the effects of enkephalins and morphine were antagonized by naloxone (2 mg/kg, i.v. in vivo and 10(-6)M in vitro). In vivo, the enkephalins (0.01 mg/kg i.v.) and morphine (2 mg/kg, i.v.) decreased the amplitude of the excitatory responses evoked in the sphincter by stimulation of the hypogastric nerves. Opiates presumably act on the sympathetic nerve endings by reducing the release of noradrenaline. In vitro, the enkephalins (10(-6)M) and morphine (10(-6)M) had a similar inhibitory effect, indicating that opiates act, at least partly, at intramural level. In vivo, the enkephalins and morphine produced an inhibition of the spontaneous electrical activity of the internal anal sphincter. This inhibition occurs also in vitro; it is thus due to a peripheral effect of opiates acting either directly on the sphincteric smooth muscle cells, or through the nervous structures controlling sphincteric motility. In addition, the distribution of nerves containing enkephalin-like immunoreactivity, using whole mount preparations of cat internal anal sphincter, indicates that this area is supplied with a dense Leu- and Met-enkephalinergic innervation. Met- and Leu-enkephalin-like immunoreactive axons were detected within the circular and longitudinal muscles.
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D001003:Anal Canal / Q000187:drug effects* / Q000294:innervation / Q000502:physiology; D000818:Animals; D001369:Axons / Q000032:analysis; D002415:Cats; D004558:Electric Stimulation; D004576:Electromyography; D004745:Enkephalins / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D007001:Hypogastric Plexus / Q000502:physiology; D008297:Male; D009020:Morphine / Q000494:pharmacology / Q000502:physiology*; D009130:Muscle, Smooth / Q000294:innervation; D009270:Naloxone / Q000494:pharmacology
chemical_list: D004745:Enkephalins; D009270:Naloxone; D009020:Morphine