Evaluation of the actin filament length from the time course of the depolymerization process.
When pyrenyl-labelled actin, at intermediate stages of polymerization, is diluted in the polymerization buffer, the decrease of fluorescence takes place stepwise through pseudo zero order reactions of decreasing rate. It is shown that the analysis of the kinetic course of the reaction allows the evaluation of the length of the actin filaments.
D000199:Actins; D000818:Animals; D005453:Fluorescence; D007700:Kinetics; D011108:Polymers; D011817:Rabbits
D000199:Actins; D011108:Polymers