The intestinal metabolism and DNA binding of benzo[a]pyrene in guinea-pigs fed normal, high-fat and high-cholesterol diets.
Strains of intestinal bacteria were capable of deconjugating benzo[a]pyrene metabolites in vitro. The hydrolysis products, and other primary oxidative metabolites of benzo[a]pyrene, were stable to further degradation by the strains tested. Cytochromes P-450 and b5 were detectable in the mucosa of the guinea-pig small intestine, but not in the mucosae of the colon or rectum. The concentrations were unaltered by administration of benzo[a]pyrene and/or the feeding of high-fat or high-cholesterol diets. Benzo[a]pyrene hydroxylase was measurable in the mucosa of the upper intestine, but was present in the lower gut only at very low levels in some animals. The activity was inducible, by oral administration of benzo[a]pyrene, in the small intestinal mucosa of guinea-pigs fed normal diet but not in those fed high-fat and high-cholesterol diets. Low levels of covalent binding of 3H to DNA of liver and gut mucosa were obtained in guinea-pigs dosed orally with 3H-benzo[a]pyrene. Comparison with data for animals given 3H2O suggested that approx. one quarter of the binding was probably due to 3H exchange during metabolism. The feeding of high-fat and high-cholesterol diets did not increase this binding. Guinea-pigs fed high-fat and high-cholesterol diets excreted a greater proportion of an oral dose of 3H-benzo[a]pyrene in urine, and less in faeces than animals fed a normal diet. Due to the low, and apparently non-inducible, levels of benzo[a]pyrene hydroxylase activity and of covalent binding in the colonic mucosa, the administration of benzo[a]pyrene to guinea-pigs fed high-fat or high-cholesterol diets appears unlikely to provide a novel animal model for studies on mechanisms of colon carcinogenesis.
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D003106:Colon / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004041:Dietary Fats / Q000494:pharmacology*; D005965:Glucuronates / Q000378:metabolism; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000201:enzymology / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D008297:Male; D008861:Microsomes / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
D002791:Cholesterol, Dietary; D003580:Cytochromes; D004041:Dietary Fats; D005965:Glucuronates; D013431:Sulfates; D001564:Benzo(a)pyrene; D004247:DNA