[Fluorescence proper of phospholipase A2 and orientotoxin from the venom of the giant hornet in the presence of ionic modulators of their membrane activity].
The own fluorescence of phospholipase A2 and orientotoxin at interaction of these components from Oriental hornet venom with calcium, strontium, lantan, magnesium ions has been investigated. Ca2+, La3+, Sr2+--activators of phospholipase activity--increase the quantum output of fluorescence. In the presence of Ca2+ the temperature of semitransitional process of thermoinactivation of investigated polypeptides increases by 2 degrees C.
D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005453:Fluorescence; D007811:Lanthanum / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology*; D010741:Phospholipases A / Q000032:analysis / Q000494:pharmacology*; D054467:Phospholipases A2; D013050:Spectrometry, Fluorescence; D013324:Strontium / Q000494:pharmacology; D013696:Temperature; D014862:Wasp Venoms / Q000032:analysis / Q000494:pharmacology*
D001514:Bee Venoms; D014862:Wasp Venoms; D007811:Lanthanum; C042190:orientotoxin; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D008274:Magnesium; D002118:Calcium; D013324:Strontium
Issledovanie sobstvennoĭ fluorestsentsii fosfolipazy A2 i orientotoksina iz iada bol'shogo shershnia v prisutstvii ionov--modulia-torov ikh membrannoĭ aktivnosti.