A test bed for ocular drugs.
Corneal endothelium plays a vital role in maintaining corneal transparency. We describe here a new in vitro technique for detecting acute effects of ocular drugs on corneal endothelium from animal species and post-mortem human tissue. The technique is based upon trans-endothelial electrical activity and leads as a consequence, to rapid, unambiguous, quantitative assessments of pharmacological effects upon endothelial barrier function and fluid pump activity.
D000818:Animals; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002918:Chymotrypsin / Q000494:pharmacology; D003315:Cornea / Q000187:drug effects* / Q000502:physiology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004727:Endothelium / Q000187:drug effects / Q000502:physiology; D004837:Epinephrine / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D010042:Ouabain / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors
D002257:Carbonic Anhydrase Inhibitors; D000431:Ethanol; D010042:Ouabain; D002918:Chymotrypsin; D004837:Epinephrine