The effect of sodium thiosulfate on subcellular localization of platinum in rat kidney after treatment with cisplatin.
Female Sprague-Dawley rats were given 10 mg/kg of i.v. cis-diammine-dichloroplatinum(II) (cisplatin) simultaneously with i.v. sodium thiosulfate (STS) at a 200-fold molar excess to cisplatin, then subcellular (nuclei, mitochondria, microsomes, cytosol) distribution of platinum within rat kidney cells was determined 15 min, 1 h, 8 h and 24 h after cisplatin injection. Blood urea nitrogen levels were measured in rats treated in the same manner described above. STS was found to block cisplatin-induced nephrotoxicity. However, differences in platinum concentrations in total homogenate or each subcellular fraction between STS-treated rats and controls were not significant enough to fully account for the drastic protective effect of STS against cisplatin nephrotoxicity.
D000818:Animals; D001806:Blood Urea Nitrogen; D002945:Cisplatin / Q000378:metabolism* / Q000633:toxicity; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010984:Platinum / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013885:Thiosulfates / Q000494:pharmacology*
D013885:Thiosulfates; D010984:Platinum; C017717:sodium thiosulfate; D002945:Cisplatin