[Study of alveolar macrophages stimulated with zymosan in vivo using fluorescent probes].
Zymosan granules injected intravenously (100 mg per kg of body weight) to Wistar rats induced strong stimulation of mononuclear phagocyte system. On day 5 zymosan-induced recruitment of monocyte-like cells with high consuming and bactericidal activity into lung spaces could be observed. Using fluorescent probes, it was discovered that the size of alveolar cell membranes in zymosan-stimulated or non-stimulated animals was similar, while the viscosity of membrane lipids of alveolar macrophages was considerably lower in zymosan-stimulated rats. These variations are suggested to be responsible for antiinflammatory and bactericidal macrophage potentials.
D000818:Animals; D005456:Fluorescent Dyes; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000378:metabolism*; D008560:Membrane Fluidity; D011650:Pulmonary Alveoli / Q000166:cytology / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D015054:Zymosan / Q000494:pharmacology*
D005456:Fluorescent Dyes; D015054:Zymosan
Izuchenie al'veoliarnykh makrofagov, stimulirovannykh zimozanom in vivo, s pomoshch'iu fliuorestsentnykh zondov.