Derivatization of endogenous and exogenous compounds in plasma for high-performance liquid chromatographic analysis.
Methods are described for the analysis of endogenous (acetoacetic and pyruvic acids) and exogenous (propranolol) compounds in plasma. Acetoacetic and pyruvic acids were converted into 2,4-dinitrophenylhydrazones and identified by chromatography on a normal-phase silica gel column at 360 nm. The propranolol amino group was modified with dansyl chloride and the dansyl derivative, after extraction with a hydrophobic solvent, was analysed on a normal-phase silica gel column using fluorescence detection. The method allows the determination of 0.5 ng/ml of propranolol.
D000090:Acetoacetates / Q000097:blood; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007651:Keto Acids / Q000097:blood*; D004364:Pharmaceutical Preparations / Q000097:blood*; D010659:Phenylhydrazines; D011433:Propranolol / Q000097:blood; D011773:Pyruvates / Q000097:blood; D019289:Pyruvic Acid
D000090:Acetoacetates; D007202:Indicators and Reagents; D007651:Keto Acids; D004364:Pharmaceutical Preparations; D010659:Phenylhydrazines; D011773:Pyruvates; C004787:2,4-dinitrophenylhydrazine; D019289:Pyruvic Acid; D011433:Propranolol