Identification by message selection of cDNA clones encoding antigens of Schistosoma mansoni.
cDNA clone banks have been constructed in a plasmid vector (pJSC73) using mRNA isolated from adult worms and eggs of Schistosoma mansoni. These clone banks have been screened by message selection (hybrid release translation) and clones containing fragments of genes encoding schistosome antigens have been identified by immunoprecipitation of the corresponding mRNA in vitro translation product. Over 50 clones encoding schistosome antigens have been identified including those of the 100 000, 86 000, 28 000, and 27 000 molecular weight surface antigen precursors.
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004274:DNA, Recombinant; D005260:Female; D006801:Humans; D007814:Larva; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012550:Schistosoma mansoni / Q000235:genetics* / Q000276:immunology
D000947:Antigens, Helminth; D000954:Antigens, Surface; D004274:DNA, Recombinant; D012333:RNA, Messenger; D004247:DNA