New angle rotor coil planet centrifuge for counter-current chromatography. II. Design of the apparatus and studies on phase retention and partition capability.
A new angle rotor coil planet centrifuge (psi = 25 degrees), which produces a Type J-L synchronous planetary motion, has been constructed to examine its capability in terms of stationary phase retention and solute partitioning. Studies on phase distribution diagrams obtained from various two-phase solvent systems indicated that the present system can be adapted to a wide variety of solvent systems by adjusting the centrifugal conditions. Excellent partition capability of the apparatus was successfully demonstrated in separations of dinitrophenyl amino acid samples with chloroform-acetic acid-0.1 N hydrochloric acid (2:2:1).
D000596:Amino Acids / Q000032:analysis; D002498:Centrifugation / Q000295:instrumentation*; D002845:Chromatography / Q000295:instrumentation*; D004140:Dinitrophenols / Q000032:analysis; D007202:Indicators and Reagents; D012995:Solubility; D012997:Solvents
D000596:Amino Acids; D004140:Dinitrophenols; D007202:Indicators and Reagents; D012997:Solvents