Semiautomated analysis of ethanol and acetate in human plasma by head space gas chromatography.
Plasma samples (0.5 mL) were analyzed for ethanol and acetate by head space gas chromatography using a Porapak QS column (80-100 mesh). Acetate was esterified to methyl acetate simply by the addition of acidified methanol. The analytical ranges were 1.61-103 and 0.05-1.9 mM for ethanol and acetate, respectively. The within-run coefficients of variation did not exceed 4.7% for acetate and 2.7% for ethanol. After the oral administration of ethanol to two healthy human subjects, the concentration versus time profiles of plasma ethanol and acetate were determined. Acetate concentrations (0.4-0.9 mM) remained quite constant while ethanol was being metabolized and appeared not to be affected by the concentration of ethanol in the range 3-18 mM. The advantages of the method are speed and simplicity.
D000085:Acetates / Q000097:blood*; D001322:Autoanalysis; D002849:Chromatography, Gas; D000431:Ethanol / Q000097:blood*; D006801:Humans
D000085:Acetates; D000431:Ethanol