A procedure for the immunoblotting of proteins separated on isoelectric focusing gels.
A method has been devised for performing Western blot assays on proteins resolved by isoelectric focusing. Electrophoretic transfer of proteins directly from isoelectric focusing (IEF) tube gels to nitrocellulose sheets allowed their immunoassay without conventional second dimension SDS gel electrophoresis. The same method can also be used for IEF slab gels. For the immunostaining of nonmuscle actin isoforms in extracts of cultured cells, the resolution of this technique was much improved over that of Western blots of two-dimensional gels.
D000199:Actins / Q000302:isolation & purification; D003101:Collodion; D007525:Isoelectric Focusing / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*
D000199:Actins; D011506:Proteins; D003101:Collodion