Synthesis and NMR spectra of 13C-labeled coenzyme A esters.
The synthesis of coenzyme A thioesters of 13C-labeled acetate, propionate, succinate, and methyl malonate is described. The average yields were 94%. The 13C-NMR spectra were determined to provide a reference for the resonance positions of these metabolites. The synthesis of coenzyme thioesters of small-molecular-weight acids labeled with 13C has not been described previously, nor have the resonance positions been previously reported.
D000214:Acyl Coenzyme A / Q000032:analysis / Q000138:chemical synthesis; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D008316:Malonyl Coenzyme A / Q000031:analogs & derivatives
D000214:Acyl Coenzyme A; D002247:Carbon Isotopes; C015357:methylmalonyl-coenzyme A; C009061:propionyl-coenzyme A; D008316:Malonyl Coenzyme A; C012046:succinyl-coenzyme A