Immunological activation of rainbow trout macrophages induced in vitro by sperm autoantibodies and factors derived from testis sensitised leucocytes.
Young cultures of rainbow trout macrophages were found to significantly increase in diameter following addition of muramyl dipeptide or of supernatants from testis/FCA sensitised leucocytes incubated with spermatozoa, or a testis extract. However, such supernatants caused little migration inhibition or increase in phagocytosis of spermatozoa by macrophages, whereas opsonization of spermatozoa with anti-sperm serum caused an almost six-fold increase in phagocytosis. These results suggest an interplay between humoral and cell-mediated immune reactions upon macrophages during experimental autoimmunity to the testis in trout.
D000818:Animals; D001323:Autoantibodies / Q000502:physiology*; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D007962:Leukocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D010587:Phagocytosis; D012487:Salmonidae / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*; D013737:Testis / Q000276:immunology; D014337:Trout / Q000276:immunology*
D001323:Autoantibodies