The preparation and properties of a highly specific antiserum elicited with 3-dehydrocholylglycine 3-succinyl-bovine serum albumin conjugate.
This report describes the synthesis of a new cholylglycine derivative-bovine serum albumin conjugate. The hapten is linked to the carrier protein at the C-3 position, through a hemisuccinate bridge. Antiserum elicited by this antigen is highly specific to cholylglycine. Cross-reactions with free cholic acid (less than 0.1%) or cholyltaurine (0.5%) are minimal.
D055598:Chemical Phenomena; D002621:Chemistry; D006000:Glycocholic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology*; D007106:Immune Sera / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013386:Succinates / Q000032:analysis*
C050384:3-succinyl-3-dehydrocholylglycine-bovine serum albumin conjugate; D007106:Immune Sera; D013386:Succinates; D012710:Serum Albumin, Bovine; D006000:Glycocholic Acid