[Disordered DNA repair in tumor cells as affected by sarcolysine].
Sarcolysine-induced damaging and reparative processes in the primary structure of tumour cell DNA have been studied. The presence of low sarcolysine concentrations (1 mkM) in the cell culture during the first two hours of incubation caused suturing of DNA molecules. The increase of the incubation time from 4 to 18 hours and the rise in the drug concentration (by 10.20 times and more) resulted in intensive accumulation of one-strand breaks. However, we have not observed the appearance of high-molecular DNA, which is the evidence of the completion of the reparative process. The impulse treatment with sarcolysine (1 hour, 10 mkM) with subsequent drug removal caused the irreversible damage of DNA reparative processes at the stage of short fragments' suturing.
D000818:Animals; D004260:DNA Repair / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008297:Male; D008558:Melphalan / Q000627:therapeutic use*; D051379:Mice; D012511:Sarcoma 37 / Q000188:drug therapy* / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000188:drug therapy*; D013997:Time Factors
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D008558:Melphalan
Narushenie reparatsii DNK opukholevykh kletok pod deĭstviem sarkolizina.