[Monoclonal immunoglobulins to the antigens of the Japanese encephalitis virus].
Six hybridomas of the EJ series producing monoclonal antibodies to Japanese encephalitis virus antigens were generated by hybridization of immune splenocytes with the parental line of mouse myeloma cells NS-0, and one hybridoma (EJ-10) with the X63-Ag8/653 line. Among 7 species of monoclonal antibodies examined by Ouchterlony method, 3 were identified as IgM and 4 as IgG. The highest clone-producing efficacy was shown by hybridoma EJ-10 generated on the basis of X-653 cells and the least by hybridoma EJ-20. The hybrid cells readily established in the cavity of mice producing ascitic tumors in 37%-86% cases. Among the derived clones, two were found, EJ-4 (IgG) and EJ-19 (IgM), to possess a high growth potential, satisfactory clone-producing efficacy, a high per cent of positive clones in recloning, and stable production of antiviral monoclonal antibody. Hybridomas EJ-4 and EJ-19 demonstrated a marked capacity for mouse-to-mouse transmission in serial passages providing for preparative accumulation of these monospecific immunoglobulins.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007136:Immunoglobulins
Monoklonal'nye immunoglobuliny k antigenam virusa iaponskogo éntsefalita.