Rapid preparation of bacteriophage DNA for sequence analysis in sets of 96 clones, using filtration.
A method is described for the preparation of single-stranded DNA from clones in bacteriophage M13 vectors. This procedure allows multiples of 96 clones to be processed at once, utilizing filtration to remove host cells and simplifying the treatment of bacteriophage pellets. The DNA produced can be used for sequencing of mutagenesis.
D001435:Bacteriophages / Q000032:analysis*; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration; D009154:Mutation
D004279:DNA, Viral