Increased uterine uptake and nuclear retention of [3H]oestradiol through the oestrous cycle and enhanced end-organ sensitivity to oestrogen stimulation in vitamin B6 deficient rats.
Vitamin B6 deficient female rats showed a significantly earlier, greater and more prolonged uptake of a tracer dose of [3H]oestradiol into the uterus, with increased nuclear accumulation, compared with vitamin B6 supplemented animals. This was most marked at oestrus, with little difference at anoestrus. The responses to low doses of ethynyl-oestradiol were greater in ovariectomized deficient animals than in those receiving the supplemented diet, with an increased uterotrophic response and greater induction of peroxidase. In the deficient animals there was virtually complete suppression of LH secretion at doses of ethynyl-oestradiol that had no effect in controls. At high doses of ethynyl-oestradiol there was no difference between the two groups of animals. The results suggest that increased uterine uptake and accumulation of [3H]oestradiol in vitamin B6 deficiency is associated with enhanced end-organ responsiveness to sub-maximal oestrogen stimulation, and that pyridoxal phosphate may have a coenzyme role in oestrogen action.
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D004971:Estrus; D004997:Ethinyl Estradiol / Q000494:pharmacology; D005260:Female; D007986:Luteinizing Hormone / Q000378:metabolism; D010052:Ovariectomy; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000378:metabolism*; D026681:Vitamin B 6 Deficiency / Q000378:metabolism*
D004997:Ethinyl Estradiol; D004958:Estradiol; D007986:Luteinizing Hormone; D003402:Creatine Kinase