The dopamine response in mouse neuroblastoma cells is mediated by serotonin 5HT3 receptors.
Serotonin (5HT) and dopamine (DA) induce, in neuroblastoma N1E-115 cells, a transient membrane depolarization associated with an inward current. The half-maximum response is obtained with 2 microM 5HT or 200 microM DA. The maximum response to 5HT is 2-3 times that to DA. The selective 5HT3 receptor antagonists ICS 205-930 and MDL 72222 at nanomolar concentrations block both the 5HT- and the DA-induced response. High concentrations (10 microM) of 5HT2 receptor antagonists are without effect. It is concluded that, in N1E-115 cells, 5HT and DA activate a single population of 5HT3 receptors.
D000818:Animals; D002999:Clone Cells; D004298:Dopamine / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008959:Models, Neurological; D009447:Neuroblastoma; D011985:Receptors, Serotonin / Q000032:analysis / Q000187:drug effects*; D012701:Serotonin / Q000494:pharmacology*
D011985:Receptors, Serotonin; D012701:Serotonin; D004298:Dopamine