Glyceride synthesis by a cell-bound lipase from Aspergillus niger.
A cell-bound lipase from the mould Aspergillus niger was isolated and purified to homogeneity as judged by disc electrophoresis on a Sephadex G-75 column. Molecular weight determination and electrophoretic mobility (Rf value) indicated that the enzyme differed from that secreted extracellularly. The cell-bound lipase showed a higher capacity than the extracellular enzyme for glyceride synthesis, and reacted in an opposite manner in the synthetic reaction compared with the extracellular enzyme.
D001234:Aspergillus niger / Q000201:enzymology*; D004589:Electrophoresis, Disc; D005989:Glycerides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D008970:Molecular Weight
D005989:Glycerides; D008049:Lipase