title: Effect of osmolality on angiotensin-stimulated aldosterone production by primary cultures of bovine adrenal glomerulosa cells.
abstract: Studies were performed to examine the relationship between osmolality and aldosterone production using primary cultures of bovine adrenal glomerulosa cells. Cell monolayers were incubated under hypo- (234 mOsm), iso- (274 mOsm), or hyperosmotic (318 mOsm) conditions in the absence or presence of angiotensin II (10(-12) M to 10(-9) M). Although basal steroidogenesis was unaffected, angiotensin II-stimulated aldosterone production was inversely related to osmolality. Mannitol and NaCl were equally effective as osmotic particles. Thus, modulation of angiotensin II-stimulated aldosterone secretion produced in vivo by changes in plasma osmolality result, in part, from a direct effect on the glomerulosa cells.
mesh_terms: D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism*; D000450:Aldosterone / Q000096:biosynthesis*; D000804:Angiotensin II / Q000494:pharmacology*; D002478:Cells, Cultured; D009994:Osmolar Concentration
chemical_list: D000804:Angiotensin II; D000450:Aldosterone