[Characteristics of the submicroscopic structure of hybrid clone cells obtained by the fusion of mouse hepatoma cells and mink fibroblasts and containing different numbers of mink chromosomes].
Four hybrid clones obtained by fusion of mouse hepatoma cells with mink fibroblasts treated with polyethyleneglycol were studied morphologically and morphometrically using electron microscopy. The clones studied contained a double set of mouse chromosomes and different numbers of mink chromosomes. It is demonstrated that clones containing different mink chromosomes differ considerably from each other and from the parental cells in the manifestation of some morphological characters (form and type of cell growth, form of the nucleus, structure of mitochondria, distribution of membranes of the granular endoplasmic reticulum), as well as in some quantitative parametres of organelles (area of the cut of the cell and of the nucleus, a relative volume of the nucleus). The data obtained witness for the fact that some morphological traits characteristic of cells of a certain parental type may appear in hybrids independently of each other, and that the degree of their manifestation may depend on the number of chromosomes of one of the parents or, possibly, on one particular chromosome.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002875:Chromosomes / Q000648:ultrastructure*; D002999:Clone Cells / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure; D005838:Genotype; D006822:Hybrid Cells / Q000648:ultrastructure*; D007621:Karyotyping; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D008907:Mink / Q000033:anatomy & histology*
Osobennosti sumbikroskopicheskogo stroeniia kletok gibridnykh klonov, poluchennykh ot sliianiia kletok gepatomy myshi i fibroblastov norki i soderzhashchikh raznoe chislo khromosom norki.