Platelet-activating factor in normal rat uterus.
Platelet-activating factor (PAF) was found in normal rat uterus and identified as 1-0-hexadecyl/octadecenyl-2-acetyl-sn-glycero-3-phosphocholine. PAF was purified by several successive chromatographic procedures. It showed platelet aggregating activity, which was inhibited by CV 3988, and had no effect on platelets desensitized with 1-0-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine. The tert-butyldimethyl-silylderivative of 1-0-alkyl-2-acetyl-sn-glycerol, which was obtained by hydrolysis of uterine PAF with phospholipase C, was analyzed by gas chromatography-mass spectrometry. One rat uterus contained approximately 21.3 ng of 1-0-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine. This is the first report of the occurrence of a significant amount of PAF in a normal animal tissue.
D000818:Animals; D005233:Fatty Alcohols / Q000032:analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D010955:Plasmalogens / Q000378:metabolism; D010972:Platelet Activating Factor / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D014599:Uterus / Q000502:physiology*
D005233:Fatty Alcohols; D010955:Plasmalogens; D010972:Platelet Activating Factor