title: Wheat germ agglutinin binding sites in the adult mouse cerebellum: light and electron microscopic studies.
abstract: The binding properties of derivatized wheat germ agglutinin (WGA) have been examined in fixed tissue sections from the adult mouse cerebellum and also in axonal tracing paradigms following cerebellar injections. The aim of these studies is to begin to distinguish the roles different binding sites may play in generating diverse biological activities which lead to neuronal uptake and axonal transport of lectins or glycoconjugates. Vibratome sections from aldehyde-fixed cerebellum were incubated in N-[acetyl-3H] WGA or WGA conjugated to horseradish peroxidase (WGA-HRP). Sections from this in vitro binding paradigm and those from cerebellar pressure injection cases using those tracers (in vivo binding paradigm) were processed for light microscopic autoradiography, histochemistry, and electron microscopy. Blocking experiments were also performed with various sugar haptens to confirm the binding specificity of these lectin preparations. Light microscopy of lectin binding patterns within the cerebellar cortex has revealed that both derivatized WGA preparations bind most intensely to the molecular layer. Within the deep cerebellar nuclei, binding is unique and produces a punctate delineation of cell bodies and dendrites. Electron microscopy revealed that these binding sites are associated with glial processes which abut the plasma membrane of deep nuclei cells. Cerebellar WGA-HRP injection sites contain labeled profiles involved in uptake and axonal transport of the labeled lectin (e.g., multivesicular and dense bodies) in addition to label associated with synapses, glia, undetermined components of the extracellular space, and neuronal plasma membranes. These sites are therefore presumed to possess a high affinity or capacity for binding derivatized WGA. Binding studies performed here thus reveal, for the first time, the existence of discrete glial sites that display an extraordinary attraction for lectins such as WGA. The roles such glial glycoconjugates play in diverse biological activities including neuronal uptake and transport of macromolecules need further study.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002525:Cerebellar Cortex / Q000378:metabolism; D002529:Cerebellar Nuclei / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism* / Q000648:ultrastructure; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009457:Neuroglia / Q000378:metabolism; D014909:Wheat Germ Agglutinins
chemical_list: D037102:Lectins; D014909:Wheat Germ Agglutinins