Action of cholecystokinin octapeptide and CCK-related peptides on neurons in inferior mesenteric ganglion of guinea pig.
The effect of cholecystokinin octapeptide (CCK8) on neurons of inferior mesenteric ganglion of the guinea pig was examined with intracellular microelectrodes. CCK8 nonsulfated pressure ejected from micropipettes resulted in a depolarization of 95% of neurons tested. The ED50 for depolarization was 1.1 +/- S.D. 0.5 pmol. The maximum depolarization averaged 9.3 mV (+/- S.D. 6.1 mV) and lasted for 99.7 sec (+/- S.D. 14.6 sec). In 73% of the cells the depolarization was associated with either an increase or a decrease in the cell input resistance; the remainder depolarized without a change in input resistance. In those cells in which the input resistance decreased during depolarization (59% of cells tested), the null potential was -36.3 +/- 9.3 mV. In those cells in which the input resistance increased during depolarization (20% of cells tested), the null potential was -103 +/- 6 mV). In those cells that depolarized without a change in input resistance (21% of cells tested) the null potential was -90.4 +/- 2.3 mV. At an equal dose, the sulfated form of CCK8 resulted in a depolarization that was 47% (+/- 26%) smaller in amplitude and 27% (+/- 21%) shorter in duration than the depolarization produced by the nonsulfated form of CCK8. The effects of both forms of CCK8 were not abolished in low calcium-high magnesium, suggesting a direct action on the postsynaptic membrane. Application of CCK8 at intervals less than 10 min resulted in marked desensitization of the response. Desensitization to CCK8 reduced by 52% (+/- 9%) the amplitude of the nerve-evoked slow excitatory postsynaptic potential in 67% of cells in which it was tested.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002766:Cholecystokinin / Q000494:pharmacology; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D005728:Ganglia, Sympathetic / Q000166:cytology* / Q000187:drug effects; D006168:Guinea Pigs; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D012844:Sincalide / Q000494:pharmacology*; D013431:Sulfates; D013758:Tetragastrin / Q000494:pharmacology
D013431:Sulfates; D013758:Tetragastrin; D002766:Cholecystokinin; D008274:Magnesium; D012844:Sincalide; D002118:Calcium