An examination of neuropeptide Y postmortem stability in an animal model simulating human autopsy conditions.
A rabbit antiserum to neuropeptide Y (NPY) was used to develop a radioimmunoassay for measuring NPY in brain tissue. By high-performance liquid chromatography, two peaks of immunoreactivity were detected in human postmortem cortex. A minor peak was seen at the void volume, while the majority of immunoreactivity comigrated with synthetic NPY standards. Using the Spokes-Koch rat model simulating human autopsy conditions, it was shown that cortical, hippocampal and striatal concentrations of NPY-like immunoreactivity are stable for up to 24 h.
D000818:Animals; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000032:analysis; D006624:Hippocampus / Q000032:analysis; D006801:Humans; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D009478:Neuropeptide Y; D011180:Postmortem Changes; D011863:Radioimmunoassay; D051381:Rats
D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y