Loss of plasma-generated vitamin D-binding protein in explants of mouse mammary gland in culture.
Selective removal of 6S plasma-generated vitamin D-binding protein from mouse mammary gland was achieved by a simple tissue culture technique. This binder was totally diluted out from the mammary explant in a time-dependent manner within 2 days of tissue culture, allowing conditions most suitable for 1,25-dihydroxyvitamin D3-receptor studies. In comparison with other methods to eliminate 6S plasma-generated vitamin D-binding protein the present technique is easy, simple and does not require the use of other vitamin D metabolites to study differential displacement of 1,25-dihydroxyvitamin D3 binding to the heterogeneous binding sites.
D000818:Animals; D002117:Calcitriol / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D008321:Mammary Glands, Animal / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D009924:Organ Culture Techniques; D013997:Time Factors; D014809:Vitamin D-Binding Protein / Q000032:analysis / Q000097:blood*
D014809:Vitamin D-Binding Protein; D002117:Calcitriol