[Determination of DNA level in human plasma].
It has been first demonstrated that DNA concentration in the plasma of healthy donors was 5 to 30 mu kg per I ml of plasma or 10-50% of DNA content in leukocytes contained in the same blood volume. DNA plasma concentration was determined by registering DNA-bisbenzimide complex fluorescence in supernatant derived from plasma containing 10% NaCl upon heating for 2-3 min at 100 degrees C. The method is simple, specific and permits the determination of DNA concentrations in 0.05-0.1 ml of plasma samples.
D004247:DNA / Q000097:blood*; D006801:Humans; D006868:Hydrolysis; D013050:Spectrometry, Fluorescence
D004247:DNA
Opredelenie kontsentratsii DNK v plazme krovi cheloveka.