title: Experimental induction of the carrier state in yearling brook trout: a model challenge protocol for IPNV immunization.
abstract: Brook trout fry (Salvelinus fontinalis) were not protected from infectious pancreatic necrosis virus (IPNV) challenge by immersion vaccination with inactivated, purified virus at concentrations of 10(7) to 10(9) pfu/ml. Mortalities in vaccinated groups were higher than for the unvaccinated control group and appeared to be dose-dependent. A challenge protocol for adult brook trout was developed for future vaccine trials. A single intraperitoneal injection of virulent, purified virus was sufficient to make long-lasting carriers of 16 month-old trout. Fish underwent a transient viremia, identified by virus isolation from plasma and leucocytes. Feces were the most reliable samples for identification of IPNV carriers by non-sacrificial testing. Many fish in the remaining infected group were still carriers 12 and 27 weeks post-infection.
mesh_terms: D000818:Animals; D002353:Carrier State / Q000662:veterinary*; D012087:Reoviridae / Q000276:immunology*; D012487:Salmonidae / Q000382:microbiology*; D014337:Trout / Q000382:microbiology*; D014611:Vaccination / Q000379:methods / Q000662:veterinary*; D014777:Virus Diseases / Q000276:immunology*