A postmeiotically expressed clone encodes lactate dehydrogenase isozyme X.
We have analysed by the DNA sequencing one of the cDNA clones, pPM459, to mRNA abundant in spermatids of mice. This clone contained 535 base pair nucleotides with a coding region for 139 amino acids and a 3' untranslated region including a single polyadenylation signal. Screening of the protein database revealed that the deduced amino acid sequence highly matched the carboxyl terminal residues 192-330 of mouse lactate dehydrogenase isozyme X (LDH-X). Taken together with our previous report which showed transcription of the message hybridizing to pPM459 after meiosis, it was demonstrated that LDH-X mRNA synthesis continued during the postmeiotic phase in spermatogenesis.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008297:Male; D008540:Meiosis; D051379:Mice; D009693:Nucleic Acid Hybridization; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics*; D013087:Spermatids / Q000032:analysis; D013091:Spermatogenesis; D013737:Testis / Q000201:enzymology*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D007770:L-Lactate Dehydrogenase