The interaction of liposomes containing intrinsic erythrocyte membrane proteins with lipid monolayers at air/water and oil/water interfaces.
The main intrinsic membrane proteins of the human erythrocyte membrane, glycophorin and the anion transporter, were isolated by extraction with Triton X-100 and ion-exchange chromatography. After removal of detergent the extract consisted of proteolipid vesicles with a lipid:protein molar ratio in the range 50-60 and a diameter of the order of 200 nm. The interaction between these vesicles and dipalmitoylphosphatidylcholine (DPPC), cholesterol and cholesterol:DPPC (2:1 molar ratio) monolayers at air/water and n-decane/water interfaces has been studied. The vesicles interact with the monolayers, rapidly causing large increases in surface pressure. Limiting values of surface pressure, 39.4-43 mN . m-1 at air/water and 31.5-33.4 mN . m-1 at the n-decane/water interface, were reached at protein levels above 1 microgram . ml-1. At the air/water interface, and probably at the n-decane/water, surface pressure increases were limited by monolayer collapse. Compression isotherms and surface potential measurements indicated that material from the proteolipid vesicles entered the monolayer phase. In contrast to proteolipid vesicles, injection of protein-free liposomes beneath the monolayer resulted in smaller, slower increases in surface pressure. Thus, the presence of intrinsic membrane proteins in vesicles greatly facilitated the transfer of material into the lipid monolayer.
D000388:Air; D000473:Alkanes; D001457:Anion Exchange Protein 1, Erythrocyte; D055592:Biophysical Phenomena; D001703:Biophysics; D002784:Cholesterol; D004910:Erythrocyte Membrane; D006021:Glycophorins; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D011092:Polyethylene Glycols; D011663:Pulmonary Surfactants; D012795:Sialoglycoproteins; D014867:Water
D000473:Alkanes; D001457:Anion Exchange Protein 1, Erythrocyte; D006021:Glycophorins; D008081:Liposomes; D011663:Pulmonary Surfactants; D012795:Sialoglycoproteins; D014867:Water; D011092:Polyethylene Glycols; D002784:Cholesterol; C012867:decane