The localization of a lectin-like component on the Leishmania cell surface.
The binding between macrophage-like cells J774G8 and Leishmania braziliensis (NR) promastigotes was studied 'in vitro' by a radioisotopic assay under various conditions in the absence of serum. Different sugars, N-acetyl-D-glucosamine, D-glucose, D-mannose, D-galactose, and chitin, diminished the binding of the parasite, whereas other sugars, D-arabinose, D-fucose and D-xylose, did not affect the binding. The presence of a lectin-like ligand specific for N-acetyl-D-glucosamine has been detected on the cell surface of the Leishmania braziliensis (NR) by fluorescence microscopy. These data suggest that the binding of the parasite to the host's cell is a ligand-receptor interaction which involves the participation of a lectin-like component on the parasite cell surface.
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D006790:Host-Parasite Interactions / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000032:analysis*; D007891:Leishmania / Q000502:physiology*; D007892:Leishmania braziliensis / Q000187:drug effects / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology
D002241:Carbohydrates; D037102:Lectins