title: Monoclonal antibody to macrophages (EMB/11) labels macrophages and microglial cells in human brain.
abstract: Normal and diseased human central nervous system (CNS) tissues were studied immunohistochemically by a monoclonal antibody to human macrophages (EBM/11), antisera to glial fibrillary acidic protein (anti-GFAP), and alpha-1-antichymotrypsin (alpha 1-ACT). EBM/11 reacted with brain macrophages located mainly around blood vessels in normal brain; it also reacted with resting microglia in normal brain and with numerous reactive microglia and macrophages in brain tumours and inflammatory lesions. Microglia did not react with anti-GFAP or alpha 1-ACT. An EBM/11 positive phenotype, therefore, is shared by microglia and macrophages and suggests that microglial cells form a specialised part of the mononuclear phagocyte system.
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D001253:Astrocytes / Q000276:immunology; D001254:Astrocytoma / Q000276:immunology; D001921:Brain / Q000276:immunology*; D001932:Brain Neoplasms / Q000276:immunology; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000276:immunology; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology; D005910:Glioma / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D009457:Neuroglia / Q000276:immunology*; D014361:Trypsin Inhibitors / Q000276:immunology; D000514:alpha 1-Antichymotrypsin
chemical_list: D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D014361:Trypsin Inhibitors; D000514:alpha 1-Antichymotrypsin; D002918:Chymotrypsin