Characterization of multiple epoxide hydrolase activities in mouse liver nuclear envelope.
A nuclear envelope-associated epoxide hydrolase in mouse liver that hydrates trans-stilbene oxide has been identified and characterized. This epoxide hydrolase is distinct from the enzyme in nuclear envelopes that hydrates benzo[a]pyrene 4,5-oxide and other arene oxides. This distinction was demonstrated by the criteria of pH optima, response to specific inhibitors in vitro, and precipitation by specific antibodies. The new epoxide hydrolase had a pH optimum of 6.8, was poorly inhibited by trichloropropene oxide, was potently inhibited by 4-phenylchalcone oxide, and did not bind to antiserum against benzo[a]pyrene 4,5-oxide hydrolase. This nuclear enzyme is similar in many of its properties to cytosolic and microsomal trans-stilbene oxide hydrolases and may be nuclear envelope-bound form of these other epoxide hydrolases. It differed from these other trans-stilbene oxide hydrolases in that its affinities for both trans-stilbene oxide (measured as apparent Km) and 4-phenylchalcone oxide (measured as I50) were 4- to 20-fold lower than those of either the cytosolic or microsomal forms.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology*; D002599:Chalcone / Q000031:analogs & derivatives / Q000494:pharmacology; D047188:Chalcones; D004851:Epoxide Hydrolases / Q000032:analysis* / Q000037:antagonists & inhibitors; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013267:Stilbenes / Q000378:metabolism; D014239:Trichloroepoxypropane / Q000494:pharmacology
D001580:Benzopyrenes; D047188:Chalcones; D013267:Stilbenes; D014239:Trichloroepoxypropane; C006707:benzo(a)pyrene 4,5-epoxide; C028770:chalcone epoxide; D002599:Chalcone; D004851:Epoxide Hydrolases; C025906:stilbene oxide