Economical modification of a radioimmunoassay of digoxin with negligible interference from cord and neonatal sera.
The Amerlex digoxin radioimmunoassay was modified to reduce the running cost by five times. The modified method compared well with the original method (y = 1.0138x + 0.00916, r = 0.9936). Using the modified method, the performance with the American Association for Clinical Chemistry quality control program was consistently satisfactory for 1 year. When normal cord and neonatal sera were tested with the modified method, 41% of the 22 samples of cord serum and 68% of the 19 samples of neonatal serum produced digoxin levels less than or equal to 0.05 ng/ml. The highest digoxin level produced by cord and neonatal sera was 0.3 ng/ml.
D004077:Digoxin / Q000097:blood*; D005312:Fetal Blood / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D011863:Radioimmunoassay / Q000191:economics / Q000379:methods*
D004077:Digoxin