title: Protein degradation by rumen microbes of heat-treated whole cottonseed.
abstract: In vitro and in situ rumen digestion trials were conducted to measure the effect of heat treatment on protein degradation by rumen microbes of cottonseed autoclaved and dry-heated (120, 140, 160 and 180 C) for different lengths of time (20, 40, 60, 90 and 120 min). Autoclaving (120 C, 1 kg/cm2 of pressure for 60 min) was effective in reducing ammonia nitrogen concentration in vitro, but dry heat at this temperature was without effect. Dry heating caused a linear decrease of in vitro ammonia concentration as temperature increased from 140 to 180 C and as exposure time increased from 20 to 120 min. The in situ results corroborated the in vitro findings. Crude protein disappearance from dacron bags decreased from 87 to 48% as temperature increased from 140 to 180 C after 20 min of heating.
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003369:Cottonseed Oil; D004044:Dietary Proteins / Q000378:metabolism; D004063:Digestion; D006358:Hot Temperature; D010940:Plant Proteins / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*
chemical_list: D003369:Cottonseed Oil; D004044:Dietary Proteins; D010940:Plant Proteins; D000641:Ammonia