Thermotropic properties of human erythrocyte membrane proteins as affected by hydroxychloroaromatic compounds.
The thermal stability of the anion transport protein (band 3) and other proteins of the human erythrocyte membrane, as influenced by hydroxychloroaromatic (HO-Cl2-Ar) compounds, was studied by differential scanning calorimetry. Various hydroxychlorodiphenyl ethers (HO-Clx-DPEs) and hexachlorophene, but not pentachlorophenol, caused a marked decrease in the thermal stability of band 3. Most of the other calorimetric transitions of the erythrocyte membrane were only slightly affected. The activity of HO-Clx-DPEs toward lowering the transition temperature of band 3 generally increased with the degree of chlorination, and was somewhat dependent on the position of hydroxyl substitution. At higher concentrations of HO-Clx-DPEs, there was a decrease in the enthalpy change and a broadening of the endothermic transition of band 3. The order of effectiveness of these compounds, as determined from band 3 denaturation temperatures, was similar to the order of potency previously observed for hemolysis of human erythrocytes.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives; D001798:Blood Proteins / Q000187:drug effects*; D002152:Calorimetry, Differential Scanning; D002733:Chlorophenols / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D006582:Hexachlorophene / Q000097:blood / Q000494:pharmacology*; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000097:blood / Q000494:pharmacology*; D008565:Membrane Proteins / Q000097:blood*; D010416:Pentachlorophenol / Q000097:blood / Q000494:pharmacology*; D010647:Phenyl Ethers / Q000097:blood / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects; D013816:Thermodynamics
D001798:Blood Proteins; D002733:Chlorophenols; D006843:Hydrocarbons, Chlorinated; D008565:Membrane Proteins; D010647:Phenyl Ethers; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D010416:Pentachlorophenol; D006582:Hexachlorophene; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid