Inverse relationship of ethane or n-pentane and malondialdehyde formed during lipid peroxidation in rat liver microsomes with different oxygen concentrations.
When we incubated rat liver microsomes with ferrous ions and an NADPH-regenerating system, ethane and n-pentane formation increased correspondingly with decreasing concentrations of oxygen in the atmosphere above the incubation, whereas malondialdehyde increased with increasing oxygen concentrations up to a plateau. At very low oxygen concentrations - 100% helium as atmosphere, but presumably traces of oxygen were present in the microsomes - ethane and n-pentane formation were maximal and dependent on the concentrations of ferrous ions, in the case of ethane, a peak being reached at about 20 microM Fe2+, whereas n-pentane continuously increased with increasing concentrations of Fe2+. It is suggested that the inverse relationship of ethane or n-pentane and malondialdehyde is due to two different reaction sequences of microsomal lipid peroxidation with different oxygen sensitivities.
D000818:Animals; D004980:Ethane / Q000378:metabolism; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D010420:Pentanes / Q000378:metabolism; D051381:Rats
D008054:Lipid Peroxides; D010420:Pentanes; C033353:pentane; D008315:Malondialdehyde; D004980:Ethane; D010100:Oxygen