Expression of F plasmid traT: independence of traY----Z promoter and traJ control.
We have shown, using an F-derived Tra+ cosmid in conjunction with the infected-cell translational system and a time-course study, that one of the surface exclusion genes, traT, can be expressed independently of the promoter of the traY----Z operon, PYZ, and in the absence of a normal quantity of traJ gene product. Studies with deleted derivatives of the cosmid pRS2405 confirmed this independence and also indicated that expression of traD can be independent of PYZ. We propose that the expression of traT by these deleted plasmids is directed from a traJ-independent promoter, PT, located adjacent to traT.
D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D005144:F Factor; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic
D001426:Bacterial Proteins; D008565:Membrane Proteins