[Changes in the level of glucocorticoid receptors in rat tissues on exogenous and endogenous increases in glucocorticoid levels].
Effects of exogenous hydrocortisone as well as endogenous increase in content of corticosterone on the level of glucocorticoid receptors were studied on liver and spleen tissues of intact Wistar rats (100-120 g). Estimation of the glucocorticoid receptors by means of Scatchard plots was carried out using labelled triamcinolone acetonide. Reproducible estimation of glucocorticoid receptors was demonstrated in tissues of animals, which were not adrenalectomized, by means of competitive replacement of natural steroids for synthetic preparations. Content of glucocorticoid receptors in liver and spleen cytosol of intact rats was equal to 145 and 90 fmol X mg-1 of protein, respectively. Within 40 min after administration of exogenous hydrocortisone content of glucocorticoid receptors was decreased 3-fold in liver tissue. After an increase in content of endogenous glucocorticoids in blood, caused by formalin, concentration of glucocorticoid receptors was decreased 1.5-fold in liver tissue and 2-fold in spleen.
D000818:Animals; D003345:Corticosterone / Q000097:blood* / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism
D011965:Receptors, Glucocorticoid; D003345:Corticosterone
Izmenenie urovnia gliukokortikoidnykh retseptorov v tkaniakh u krys pri ékzogennom i éndogennom povyshenii kontsentratsii gliukokortikoidov.