[Localization of transcription regulatory sequences. Application to the genes of the prolactin family].
We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and placental lactogen (hCS-B). We have cloned these genes and are searching within their sequences for in vitro binding sites of the human glucocorticoid receptor on the hGH-N and hCS-B genes; the in vivo activity of such DNA sequences by assaying hybrid gene expression in transfected cells; in vivo "enhancer" activity of different hPRL gene fragments linked to a marker gene and transfected in cultured cells.
D001483:Base Sequence; D004247:DNA; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D005815:Genetic Code; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D010908:Pituitary Hormones, Anterior / Q000235:genetics*; D010928:Placental Lactogen / Q000235:genetics*; D011388:Prolactin / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000032:analysis; D014158:Transcription, Genetic
D010908:Pituitary Hormones, Anterior; D011965:Receptors, Glucocorticoid; D011388:Prolactin; D013006:Growth Hormone; D004247:DNA; D010928:Placental Lactogen
Localisation des séquences régulatrices de la transcription. Application aux gènes de la famille de la prolactine.