Functional cDNA library for efficient expression of measles virus-specific gene products in primate cells.
A cDNA library designed for high-level expression of measles virus-specific gene products in mammalian cells was generated. From this library, functional clones which contained the entire protein-coding sequences of the nucleocapsid (N) and the phosphoprotein (P) genes were isolated. By DNA-mediated gene transfer into a line of simian virus 40-transformed monkey kidney cells, the N-specific cDNA was expressed into a single polypeptide of about 60,000 Mr, which was immunoprecipitated by monoclonal antibodies against the measles virus N protein. In contrast, the P-specific cDNA could be expressed into either one or two species of polypeptides of 75,000 or 70,000 Mr, both of which were immunoprecipitated by monoclonal antibodies against the measles virus P protein.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D007668:Kidney; D008459:Measles virus / Q000235:genetics* / Q000276:immunology; D010750:Phosphoproteins / Q000235:genetics*; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D014162:Transfection; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D004274:DNA, Recombinant; D004279:DNA, Viral; D010750:Phosphoproteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins; D004247:DNA