The influence of oxygenated sterol compounds on dipalmitoylphosphatidylcholine bilayer structure and packing.
Fourier Transform Infra-red and Raman Spectroscopies indicate that 7 alpha-hydroxycholesterol and 7-ketocholesterol have a diminished capacity to condense (increase the packing order of) fluid-state dipalmitoylphosphatidylcholine (DPPC) acyl chains when compared with the effects of cholesterol and the other oxidized sterols studied. DPPC head groups were also more ordered by 7-ketocholesterol over the temperature range 10 degrees - 70 degrees C. Primary effects of these sterols appear to be associated with the hydrophillic regions of the DPPC bilayer, although packing arrangements with acyl chains are also involved. Phosphate and acyl chain ester groups were observed to possess a packing order which was invariant which indicates that these may be the target groups in the interaction with 7-ketocholesterol. A surprising observation was the synergistic amplification of the effects of 7-ketocholesterol by the presence of cholesterol in the DPPC bilayer.
D015060:1,2-Dipalmitoylphosphatidylcholine; D008051:Lipid Bilayers; D008954:Models, Biological; D008968:Molecular Conformation; D013055:Spectrophotometry, Infrared; D013261:Sterols; D013329:Structure-Activity Relationship
D008051:Lipid Bilayers; D013261:Sterols; D015060:1,2-Dipalmitoylphosphatidylcholine