Liquid chromatographic determination of fluridone aquatic herbicide and its metabolite in fish and crayfish.
A residue method is described for determination of the aquatic herbicide fluridone (1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone) and its metabolite (1-methyl-3-(4-hydroxyphenyl)-5-[3-(trifluoromethyl) phenyl]-4(1H)-pyridinone) in fish and crayfish tissues. Both compounds are extracted from tissues with methanol, and the extracts are subjected to acidic hydrolysis to release conjugated forms of fluridone and the metabolite. Sample extracts are purified by liquid-liquid partitioning and Florisil Sep-Pak column chromatography. Both compounds are separated and measured by reverse phase liquid chromatography with UV detection at 313 nm. In the absence of interfering peaks, the method has a detection limit of approximately 0.04 ppm of either compound. Overall, recoveries averaged 96% for fluridone and 78% for the metabolite for all tissue types combined.
D000818:Animals; D003400:Astacoidea; D002853:Chromatography, Liquid / Q000379:methods; D005399:Fishes; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D011728:Pyridones / Q000032:analysis*
D007202:Indicators and Reagents; D011728:Pyridones; C013351:fluridone