Three distinct H-2Ks molecules differing at the carboxy terminus are expressed on a tumor from SJL/J mice.
A dimethylbenzanthracene-induced leukemia of H-2s origin expressed at least two class I molecules on the cell surface that were precipitated by anti-H-2.19, an alloantiserum prepared against the private H-2Ks specificity. Mapping studies in recombinant inbred strains along with comparisons of tryptic peptide maps and N-terminal sequences indicated that the proteins were virtually identical and probably encoded by the same class I gene. When cells were labeled in the presence of tunicamycin, the proteins precipitated by anti-H-2.19 were further resolved into three distinct peptides. Experiments were performed to determine which of these various proteins were phosphorylated and which were recognized by an anti-synthetic peptide serum directed against the ultimate C-terminus of H-2K class I molecules. The results indicate that a single class I gene from the H-2Ks region may encode three class I molecules that differ only at the C-terminus due to alternative splicing of pre-mRNA.
D000595:Amino Acid Sequence; D000818:Animals; D005786:Gene Expression Regulation; D006031:Glycosylation; D006183:H-2 Antigens / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010768:Phosphoserine / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D014415:Tunicamycin / Q000494:pharmacology
D006183:H-2 Antigens; D010446:Peptide Fragments; D012333:RNA, Messenger; D014415:Tunicamycin; D010768:Phosphoserine