Characterization of surface glycoproteins on Schistosoma mansoni adult worms by lectin affinity chromatography.
Adult Schistosoma mansoni were radiolabeled by direct radioiodination using the Bolton-Hunter reagent or by metabolic labeling using radioactive hexose precursors. Tegumental material was extracted by freeze-thaw or by incubation in the non-ionic detergent Nonidet P-40, then applied to chromatography columns containing the following immobilized lectins: Con A, lentil lectin, wheat germ agglutinin, soybean agglutinin and the agglutinins from Ricinus communis and Helix pomatia. SDS-PAGE analysis of the sugar eluates from these columns revealed the presence of 15 glycoproteins with apparent molecular weights greater than or equal to 300,000, 215,000, 168,000, 152,000, 134,000, 122,000, 108,000, 83,000, 58,000, 53,000, 46,000, 41,000, 34,000, 30,000 and 23,500. Many of the glycoproteins reacted with more than one lectin. Information about carbohydrate content and lectin binding provides a preliminary characterization of the tegumental glycoprotein antigens of adult worms.
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D001888:Boric Acids; D002021:Buffers; D002846:Chromatography, Affinity / Q000379:methods; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D011975:Receptors, Mitogen / Q000032:analysis; D012550:Schistosoma mansoni / Q000276:immunology*; D012685:Sepharose / Q000031:analogs & derivatives; D014909:Wheat Germ Agglutinins
D000947:Antigens, Helminth; D001888:Boric Acids; D002021:Buffers; D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; C028326:concanavalin A-sepharose; D012685:Sepharose; C032688:boric acid