title: Cell kinetics of normal and perturbed populations measured by incorporation of bromodeoxyuridine and flow cytometry.
abstract: Bromodeoxyuridine (BrdU) incorporation and flow cytometry have been used to measure the kinetics of V79 cells growing at different temperatures in vitro and cells of the murine Sa F tumour growing in vivo. By simultaneously measuring total DNA content and BrdU incorporation in individual cells at different times after pulse labelling with BrdU, it is a simple procedure to quantify the movement of cells through the cell cycle. The method has the advantage of speed (2 X 10(4) or more cells are analysed within a day or so of the experiment) and the ability to analyse the results in different ways. A G2 block in tumour cell progression in vivo is readily detected after a dose as low as 2 Gy. The neutron relative biological effectiveness for this G2 block is probably larger than that for tumour growth delay.
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D051379:Mice; D008808:Mice, Inbred CBA; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine