Expression and cell type--specific processing of human preproenkephalin with a vaccinia recombinant.
The posttranslational maturation of a complex precursor polyprotein, human proenkephalin, was assessed by infection of a wide spectrum of cell types with a recombinant vaccinia virus that expressed human proenkephalin. The infected cells rapidly produced both cellular and secreted Met-enkephalin immunoreactivity. Although each cell line could secrete intact proenkephalin, only a mouse pituitary line was capable of processing proenkephalin to mature enkephalin peptides. The quantity of intact proenkephalin secreted from BSC-40 cells (derived from African Green monkey kidney) was sufficient to establish the value of vaccinia virus as a mammalian cell expression vector.
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004744:Enkephalin, Methionine / Q000096:biosynthesis; D004745:Enkephalins / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014616:Vaccinia virus / Q000235:genetics*
D004745:Enkephalins; D011498:Protein Precursors; D011994:Recombinant Proteins; C029992:proenkephalin; D004744:Enkephalin, Methionine; C033660:preproenkephalin