Rhythm of suprachiasmatic nucleus 2-deoxyglucose uptake in vitro.
Using a hypothalamic slice preparation containing the suprachiasmatic nucleus (SCN) and measurement of 2-deoxy[14C]glucose (2-DG) uptake by autoradiography, we have demonstrated that, after 1 h in vitro, 2-DG uptake into the SCN is proportional to the rate of glucose utilization present in vivo at the corresponding subjective time of day and that, during an 8-h incubation in vitro, the SCN is capable of spontaneously changing its metabolic rate.
D000818:Animals; D001345:Autoradiography; D002940:Circadian Rhythm; D003847:Deoxyglucose / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D008959:Models, Neurological; D051381:Rats; D011919:Rats, Inbred Strains; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*
D003847:Deoxyglucose; D005947:Glucose