The effects of cadmium on succinate and NADH-linked substrate oxidations in rat hepatic mitochondria.
Low concentrations of cadmium (3.3-40 microM) inhibited State 3 NADH-linked respiration in rat hepatic mitochondria, but failed to release oligomycin (1 microgram) inhibited State 3 respiration, or to significantly change the State 4 rate. In the presence of succinate, 40 microM cadmium inhibited State 3 respiration by 89%, while concentrations between 3.3 and 13.3 microM stimulated State 4 respiration. Higher concentrations caused marked inhibition. In the presence of succinate, cadmium released oligomycin inhibited State 3 respiration. Cadmium (0.001-1.0 mM) did not stimulate mitochondrial ATPase activity or inhibit ferricyanide reduction, but stimulated NAD+ linked mitochondrial dehydrogenase activities and NADH oxidation. These results indicate that cadmium interacts with either the NADH dehydrogenase complex or other NADH-dependent enzymes and not solely by an uncoupling action.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D005292:Ferricyanides / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D066298:In Vitro Techniques; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013386:Succinates / Q000378:metabolism
D005292:Ferricyanides; D013386:Succinates; D002104:Cadmium; D009243:NAD; D000255:Adenosine Triphosphate; D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase