Gas chromatography of uracil herbicides by on-column methylation with trimethylanilinium hydroxide.
Substituted uracil herbicides injected into a gas chromatograph react with trimethylanilinium hydroxide to give N-methyl derivatives with good gas chromatographic properties. Maximum methylation is obtained when the molar ratio of methylating reagent to herbicide is ca 4:1. This technique for preparing derivatives provides rapid qualitative and quantitative chromatography of the substances examined. Chromatographic response was linear with increased concentration for the synthetic standard and the on-column product of uracil herbicide. The proposed derivatization method was used to analyze herbicides in formulations. The methyl derivatives were identified spectroscopically.
D002849:Chromatography, Gas / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006540:Herbicides / Q000032:analysis*; D008745:Methylation; D000644:Quaternary Ammonium Compounds; D013053:Spectrophotometry; D014498:Uracil / Q000031:analogs & derivatives*
D006540:Herbicides; D000644:Quaternary Ammonium Compounds; C009571:phenyltrimethylammonium; D014498:Uracil