Fatty acid induced hemolysis. Protective action of ceruloplasmin, albumins, thiols and vitamin C.
The hemolytic effect of saturated fatty acids increased rapidly, when the number of carbon atoms in the chain exceeded 12. At low fatty acid concentrations (less than 60 microM) the hemolytic effect decreased with increasing number of double bonds in the carbon chain (cis-form fatty acids). A more complex pattern was observed at higher fatty acid concentrations. Trans-unsaturated fatty acids were more hemolytic than cis-analogs. Ceruloplasmin, a serum protein with no fatty acid binding capacity, reduced the hemolytic effect of fatty acids; possibly by interacting with the cell membrane. Reducing compounds (thiols, vitamin C) also protected against fatty acid induced hemolysis.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D002570:Ceruloplasmin / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013045:Species Specificity; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D005230:Fatty Acids, Nonesterified; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D002570:Ceruloplasmin; D001205:Ascorbic Acid