An enzyme-linked immunosorbent assay for the detection of antibodies to Trypanosoma (T.) brucei evansi in camels (Camelus dromedarius) using peroxidase-conjugated protein A.
Serum samples from dromedary camels naturally and experimentally infected with Trypanosoma (T.) brucei evansi were examined by means of the ELISA, using either an anti-camel or a protein A conjugate. The protein A horseradish peroxidase conjugate was found to bind to camel IgG and thus to be a suitable alternative to the anti-camel conjugate. Results obtained from both tests showed that the respective values correlate significantly.
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002162:Camelus / Q000276:immunology / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000032:analysis*; D014346:Trypanosoma brucei brucei / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000276:immunology / Q000662:veterinary*
D000953:Antigens, Protozoan; D007074:Immunoglobulin G