[Micronucleus test in cultured cells--preliminary study].
In mutagenicity and carcinogenicity assays using micronucleus test, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 50 mg/kg or benzo(a)pyrene (BaP), 100 mg/kg did not increase the incidence of micronuclei in polychromatic erythrocytes in bone marrow of mice. MNNG, 0.1 microgram/ml could induce a significant increase of micronuclei with dose response in cultured V79 cells. In another experiment of Syrian hamster embryo cells, the induction of micronuclei was seen after benzo(a)pyrene, 0.1 microgram/ml treatment. The combined use of micronucleus test in vivo and in vitro can increase the sensitivity and reduce the false negativity obtained in vivo micronucleus test.
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001853:Bone Marrow; D002478:Cells, Cultured; D006224:Cricetinae; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D008297:Male; D008647:Mesocricetus; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods*
D008769:Methylnitronitrosoguanidine; D001564:Benzo(a)pyrene; D005020:Ethyl Methanesulfonate