Impaired DNA-repair synthesis in lymphocytes of breast cancer patients.
UV-C light induced DNA-repair synthesis was studied in unstimulated lymphocytes of 41 female patients (aged 33-83 years) with invasive breast cancer and of 27 healthy women (aged 37-68 years). As a parameter for the determination of the DNA-repair synthesis the incorporation of [3H] thymidine was taken in the presence of 2 mM hydroxyurea (HU). The [3H] thymidine incorporation levels were reduced in 20 of the 41 patients and in only 3 of the 27 controls. This difference between the two groups was significant. An impaired DNA-repair synthesis might be involved in the etiology of breast cancer in some patients.
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000532:radiotherapy / Q000601:surgery; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D005260:Female; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism* / Q000528:radiation effects; D008875:Middle Aged; D013936:Thymidine / Q000378:metabolism; D014466:Ultraviolet Rays
D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea