Effect of cerulenin, an inhibitor of fatty acid synthesis, on the immune cytolysis of tumor cells.
Effect of cerulenin, an inhibitor of biosynthesis of fatty acids and sterols on the immune cytolysis of tumor cells was investigated. In cytotoxicity experiment, MH134 cells pretreated with cerulenin in a range of concentrations causing minor cellular injuries were lysed by xenogeneic antiserum and complement in a significantly higher degree than non-treated MH134 cells. C3H/He mice inoculated ip with 1 X 10(5) syngeneic MH134 cells, received cerulenin on days 3 and 6, and then the antiserum against MH134 cells on days 4 and 7. Such mice survived markedly longer than 3 control groups of mice carrying MH134 tumor cells; one without treatments, one treated only with cerulenin and the other only with the antiserum. Inhibition of biosynthesis of cell membrane fatty acid and sterols, that was indicated by inhibition of 14C-acetate incorporation, is thought to enhance the susceptibility of the tumor cells to killing by antibodies and complement. Such an inhibitor of fatty acids and sterols might be a beneficial adjuvant to the adoptive cancer immunotherapy.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002569:Cerulenin / Q000494:pharmacology* / Q000627:therapeutic use; D003602:Cytotoxicity, Immunologic; D005227:Fatty Acids / Q000096:biosynthesis; D005260:Female; D007116:Immunization, Passive; D007930:Leucine / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000276:immunology* / Q000628:therapy; D051379:Mice; D008809:Mice, Inbred C3H
D000085:Acetates; D000935:Antifungal Agents; D005227:Fatty Acids; D002569:Cerulenin; D007930:Leucine; D019342:Acetic Acid