[Activation of calcium transport in synaptosomes by phosphatidylinositol-specific phospholipase C].
The isotope labeling method was used to study the influence of phospholipases C of different origin and specificity on Ca2+ accumulation in rat brain synaptosomes. It was found that phospholipases C specific to phosphatidylinositides (PI) stimulate Ca2+ transport into synaptosomes, while non-specific phospholipase C, which hydrolyzes different membrane lipid fractions, decreases the Ca2+ content in synaptosomes. It is supposed that the stimulating effect of PI-specific phospholipases C is determined by the activation of PI metabolism, which results in an increase in the content of some PI metabolism products serving as Ca2+ ionophores in synaptosomal membranes. The inhibition of Ca2+ uptake by synaptosomes treated with non-specific phospholipase C is thought to result from partial disruption of synaptosomal membranes.
D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism*
D008563:Membrane Lipids; D010716:Phosphatidylinositols; D010738:Type C Phospholipases; D002118:Calcium
Aktivatsiia transporta kal'tsiia v sinaptosomakh fosfolipazoĭ C, spetsifichnoĭ k fosfatidilinozitam.