Effects of hypoxia on the small acetylcholinesterase-positive megakaryocyte precursor in bone marrow of mice.
The number of small acetylcholinesterase-positive (SAChE+) cells in the marrow of hypoxic mice was measured. Mice were exposed to 6-7% O2 levels by enclosure in cages covered with dimethyl-silicone rubber membranes for 1-14 days. The mice showed a linear increase in packed cell volumes with time in the hypoxic atmosphere, but platelet counts showed a characteristic biphasic response, i.e., increased platelet counts were observed after 1-3 days of hypoxia, and significantly (P less than 0.05-P less than 0.0005) decreased platelet counts were observed thereafter (6-14 days). The total number of megakaryocytes in the marrow of hypoxic mice decreased significantly (P less than 0.005) with time. In agreement with the data on platelet counts, hypoxia caused the total number of SAChE+ cells in the marrow of mice to be biphasic. At Day 2 there was a significant increase (P less than 0.05) in the total number of SAChE+ cells/mm3 of bone marrow; however, by days 10-14 the numbers had decreased markedly (P less than 0.005). These data indicate that hypoxia decreases platelet production by action on a precursor cell to the SAChE+ cell. The hypoxia-induced thrombocytopenia is probably caused by stem-cell competition between the erythrocytic and megakaryocytic cell lines.
D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000473:pathology*; D000860:Hypoxia / Q000473:pathology*; D008297:Male; D008533:Megakaryocytes / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D008809:Mice, Inbred C3H; D010976:Platelet Count
D000110:Acetylcholinesterase