title: Citrate interference with the determination of acetylcarnitine: a method for its elimination.
abstract: Citrate can interfere with the determination of acetylcarnitine when measured by a radioisotopic method. In this report we present a modified procedure designed to avoid this effect. Recovery of added acetylcarnitine was approximately 95%. Acetylcarnitine in normal human plasma was found to range from 0.8 to 19.9 nmol/ml with a mean of 6.3; that of human skeletal muscle ranged from 0.1 to 1.6 nmol/mg protein with a mean of 1.08. The results confirm that exercise gives rise to increased acetylcarnitine content of plasma.
mesh_terms: D000108:Acetylcarnitine / Q000032:analysis* / Q000097:blood; D002331:Carnitine / Q000031:analogs & derivatives*; D002951:Citrates; D019343:Citric Acid; D005069:Evaluation Studies as Topic; D006801:Humans; D009132:Muscles / Q000032:analysis; D005082:Physical Exertion
chemical_list: D002951:Citrates; D019343:Citric Acid; D000108:Acetylcarnitine; D002331:Carnitine