Biological properties of Pyrularia thionin prepared from nuts of Pyrularia pubera.
Pyrularia thionin is a 47 amino acid basic peptide which resembles wheat purothionin and mistletoe viscotoxin. It is toxic to mice, with an LD50 of 1.5 mg/kg body weight and is cytotoxic to tumor and normal cells in culture, with ID50 values ranging from 0.62 to 17 micrograms/ml. Against seven bacterial species it was toxic only to Micrococcus luteus. The toxin did not protect mice against transplanted B16 melanoma, nor did it show any mitogenic activity with mouse spleen lymphocytes.
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D000972:Antineoplastic Agents, Phytogenic; D002460:Cell Line; D003603:Cytotoxins; D006461:Hemolysis / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008545:Melanoma / Q000188:drug therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D008837:Micrococcus / Q000187:drug effects; D008934:Mitogens; D010940:Plant Proteins / Q000494:pharmacology* / Q000633:toxicity
D023181:Antimicrobial Cationic Peptides; D000972:Antineoplastic Agents, Phytogenic; D003603:Cytotoxins; D008934:Mitogens; D010940:Plant Proteins; C044942:THI1 protein, Pyrularia pubera