Enzymic removal of two oligosaccharide chains from ricin B-chain.
Peptide:N-glycosidase F removed both the asparagine-linked oligosaccharide chains of ricin B-chain in the absence of lactose. In the presence of lactose, which binds specifically to the B-chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B-chain against the removal of one of the two susceptible oligosaccharides present in each B-chain subunit.
D000581:Amidohydrolases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007785:Lactose / Q000494:pharmacology; D009844:Oligosaccharides / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D012276:Ricin / Q000378:metabolism*
D009844:Oligosaccharides; D010446:Peptide Fragments; D012276:Ricin; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D007785:Lactose