[Metabolism of oligoadenylates in cell nuclei and regulation of protein ADP-ribosylation].
2',5'-Oligo(A)synthetase (2-5A) and 2-phosphodiesterase were found in the L cells nuclei. In the cell nuclei 2-5A is 10-30 times higher, than in the cytoplasm. It is induced by interferon and depends on the cell growth state. 2-Phosphodiesterase activity has two pH optima of hydrolysis of 2-5A, namely 7.1, and 7.9 and decreases after interferon treatment of cells. Thus, interferon treatment of cells leads to an increase of the 2-5A level in cell nuclei. One of the possible pathways for 2-5A action in cell nuclei is the regulation of (ADP-ribose)transferase activity. Treatment of L cells with 2-5A (A2pA2pA) leads to activation of ADP-ribosylation of proteins by a factor of 1.5 in a concentration range of 10(-9)-10(-7) M, but more higher concentrations of 2-5A inhibit this process up to 60%. Treatment of cells with actinomycin D has no influence on 2-5A induced changes in protein ADP-ribosylation. This result is indicative for a new pathway of interferon action and 2-5A mediated regulation of cell metabolism.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism; D005095:Exoribonucleases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007739:L Cells; D051379:Mice; D009843:Oligoribonucleotides / Q000378:metabolism*
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D000246:Adenosine Diphosphate Ribose; C023505:2',5'-oligoadenylate; D005095:Exoribonucleases; C029364:2'-phosphodiesterase
Metabolizm oligoadenilatov v kletochnykh iadrakh i reguliatsiia ADP-ribozilirovaniia belkov.