A procedure for the analysis of S-benzyl-N-acetylcysteine and S-(o-methylbenzyl)-N-acetylcysteine in human urine.
Analytical methods for mercapturic acids, the end-products of glutathione metabolism of the industrial solvents toluene and o-xylene (i.e., S-benzyl-N-acetylcysteine and S-(o-methylbenzyl)-N-acetylcysteine), added to human urine have been developed. The sensitivity is 2 ng/microliter (R.S.D. = +/- 13%) and 1 ng/microliter (R.S.D. = +/- 6.4%) urine for S-benzyl-N-acetylcysteine and S-(o-methylbenzyl-N-acetylcysteine, respectively, when using the h.p.l.c. method. The corresponding sensitivities for the g.l.c.-mass spectrometric method are 0.5 and 0.3 ng/microliter urine, respectively.
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
C049842:S-(2-methylbenzyl)-N-acetylcysteine; D000111:Acetylcysteine