An avidin-biotin ELISA for the detection of staphylococcal enterotoxins A and B.
The avidin-biotin system was incorporated into the enzyme-linked immunosorbent assay (ELISA) to establish a new detection method for staphylococcal enterotoxins A and B in culture supernatants. Staphylococcal protein A (SPA) does not interfere significantly with the procedure. The test shows good sensitivity for the toxins and the ease and rapidity with which the assay can be performed make it a valuable tool for the routine detection of enterotoxins.
D000469:Alkaline Phosphatase; D001360:Avidin; D001710:Biotin; D004306:Dose-Response Relationship, Immunologic; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D013211:Staphylococcus aureus / Q000276:immunology
D004768:Enterotoxins; D001360:Avidin; D001710:Biotin; D000469:Alkaline Phosphatase