Structural changes in retinol binding protein induced by retinol removal. A molecular dynamics study.
Relationships between structure and function for retinol binding protein (RBP) are elucidated with help of a 2.0 A resolution X-ray structure of the holo-protein and an average molecular dynamics (MD) structure of the apo-form. Comparisons between MD simulations of both the apo- and holo-forms with the X-ray holo-structure show conformational changes in apo-RBP that may be functionally significant. The average three dimensional structure obtained for apo-RBP is compared to the related protein apo-beta-lactoglobulin. Available biochemical information is consistent with structure/function relationships derived here.
D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D012177:Retinol-Binding Proteins / Q000032:analysis*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014801:Vitamin A / Q000502:physiology*; D014961:X-Ray Diffraction
D012177:Retinol-Binding Proteins; D014801:Vitamin A