Correlation between the molecular structure of cardiac glycosides, steroid hormones and carbohydrates and their retention in high-performance liquid chromatography.
Cardiac glycoside molecules and other molecules can be thought as consisting of a number of functional groups that make individual average contributions to the overall retention. To evaluate these contributions it was necessary to solve a system of linear equations by the last-squares method for a number of cardiac glycosides and steroid hormones, the retention volumes of which were determined under standard conditions. Experimental values of the logarithm of retention volumes are compared with calculated values, ln VR' = sigma niai, where ai = ln (VR')i is the contribution to the retention of group i and ni is the number of functional groups in a molecule. Such a comparison shows that these values are in good agreement and the method may be used to evaluate the retentions of cardiac glycosides, steroid hormones and carbohydrates. The correlation of biological activity of cardiac glycosides and the logarithm of retention volumes is expressed by a linear equation, the coefficients of which have been determined. This equation shows that biological activity is related to the hydrophilic-hydrophobic properties of cardiac glycosides and consequently with the transport of these compounds to the receptor.
D002241:Carbohydrates / Q000032:analysis*; D002301:Cardiac Glycosides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008968:Molecular Conformation; D013256:Steroids / Q000032:analysis*
D002241:Carbohydrates; D002301:Cardiac Glycosides; D013256:Steroids