Freeze-fracture cytochemistry of wheat germ agglutinin and concanavalin A receptors on the plasma membrane of normal, Bernard-Soulier, and thrombasthenic platelets.
The authors report here the results of fracture-labeling of wheat germ agglutinin (WGA) and concanavalin A (Con A) receptors on the plasma membranes of normal, Bernard-Soulier, and thrombasthenic platelets. In all cases, virtually all of the label was confined to the exoplasmic half of the membrane. Despite the absence of GP Ib in Bernard-Soulier platelets and the absence or strong reduction of Gp IIb and GP IIIa in thrombasthenic platelets, their plasma membranes were strongly labeled by both Con A and WGA. These results are best accounted for by the presence of other glycoproteins and/or glycolipids at the platelet surface.
D000328:Adult; D001606:Bernard-Soulier Syndrome / Q000097:blood*; D001791:Blood Platelet Disorders / Q000097:blood*; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D005614:Freeze Fracturing; D006023:Glycoproteins / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins; D008854:Microscopy, Electron; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D011975:Receptors, Mitogen / Q000378:metabolism*; D013915:Thrombasthenia / Q000097:blood*; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D011952:Receptors, Concanavalin A; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; C027503:wheat germ agglutinin receptor; D003208:Concanavalin A