Effects of UV-irradiation on the SCE frequency in human lymphocyte cultures.
UV-irradiation (254 nm) was found to induce a smaller increase of SCE in human lymphocytes than in human fibroblasts and CHO cells. The UV-induced SCE frequency in human lymphocytes was not influenced by the duration between irradiation and the subsequent S-phase. UV-irradiated lymphocytes showed a slightly more than additive response to the SCE-inducing effect of HN2 and acetaldehyde in comparison with non-irradiated cells. The UV-induced SCE frequency was similar in lymphocyte cultures containing 20 and 100 microM of BrdUrd. The results suggest that human lymphocytes are relatively insensitive to the SCE-inducing effect of UV-irradiation, and that SCE-inducing damage caused by UV is not removed during the G1 phase in these cells.
D000079:Acetaldehyde / Q000494:pharmacology; D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007399:Interphase; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D008466:Mechlorethamine / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects*; D014466:Ultraviolet Rays
D008466:Mechlorethamine; D001973:Bromodeoxyuridine; D000079:Acetaldehyde