Formation of didesmethylethimizol (4-5-dicarbamoyl-1-ethylimidazole) in man.
The chemical structure of an ethimizol metabolite found earlier in human urine and saliva was established as 4,5-dicarbamoyl-1-ethylimidazole (didesmethylethimizol). The treatment of biological samples comprised a solid-phase extraction, high-performance thin-layer chromatographic separation and high-performance liquid chromatographic purification techniques. The identification of the metabolite was based on the comparison of a mass spectrum of the substance isolated from the biological fluid with the mass spectra of synthetised reference compounds.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005042:Etimizol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D006801:Humans; D007093:Imidazoles; D013058:Mass Spectrometry; D008745:Methylation; D012463:Saliva / Q000032:analysis*
D007093:Imidazoles; C050742:N,N'-didesmethylethimizol; D005042:Etimizol