A column purification procedure for the removal of leucocytes from parasite-infected bovine blood.
A cellulose column procedure is described which removes white cells from bovine blood infected with Babesia and Anaplasma. The efficiency of this method was confirmed by the absence of white blood cell DNA in lysates from column-filtered infected as well as non-infected blood.
D000709:Anaplasma / Q000235:genetics; D000818:Animals; D001403:Babesia / Q000235:genetics; D002417:Cattle / Q000097:blood* / Q000382:microbiology / Q000469:parasitology; D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000382:microbiology / Q000469:parasitology*; D007962:Leukocytes
D004269:DNA, Bacterial; D004247:DNA