Bombesin stimulation of c-fos and c-myc gene expression in cultures of Swiss 3T3 cells.
Bombesin has been shown to be a potent mitogen for Swiss 3T3 cells. At nanomolar concentrations it stimulates DNA synthesis in quiescent cultures of 3T3 cells and also induces the expression of c-fos and c-myc mRNA. c-fos mRNA transcripts dramatically increase 15 min after the addition of bombesin, are still abundant after 30-60 min and then decrease. c-myc mRNA induction is detectable later, 1 h after bombesin treatment. Conversely, no changes in c-Ki-ras expression are observed after stimulation with bombesin. These results demonstrate that the increased expression of c-fos and c-myc mRNAs appears to be a common response to diverse agents that induce DNA synthesis and cell proliferation.
D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects*; D007399:Interphase; D051379:Mice; D009857:Oncogenes; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004247:DNA; D001839:Bombesin