Preparation, purification and analysis of BSA-coated latex particles for in vivo studies. Non-ionic adsorbance of unbound protein ligand.
A procedure for the preparation, purification and analysis of small, BSA-coated carboxylate-modified latex (CML) particles, suitable for use in in vivo studies has been developed. Following conjugation, uptake of unbound BSA by Amberlite XAD-8 non-ionic adsorbent beads has been shown to be an effective method by which unbound protein ligands may be removed from coated latex preparations.
D000327:Adsorption; D000818:Animals; D007840:Latex; D008024:Ligands; D008297:Male; D051381:Rats; D012117:Resins, Synthetic; D012710:Serum Albumin, Bovine; D014018:Tissue Distribution
D007840:Latex; D008024:Ligands; D012117:Resins, Synthetic; D012710:Serum Albumin, Bovine; C046797:amberlite