Cell-free synthesis of high-molecular-weight small intestinal polypeptides.
An intrinsic membrane glycoprotein, Mr 131 000, is a major developmentally specific component of the neonatal guinea-pig small intestinal microvillar membrane. Such high-molecular-weight proteins are often difficult to translate in vitro. In this study we report a successful strategy for the identification of the primary translation product of this glycoprotein, a high-molecular-weight precursor polypeptide of approximate Mr 225 000.
D000818:Animals; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D007421:Intestine, Small / Q000648:ultrastructure*; D008871:Microvilli / Q000378:metabolism; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis