Measurement of DNA content in single cells morphologically identified on smears.
A method was developed for measuring the nuclear DNA content in single cells previously identified on a bone marrow smear stained by the Wright-Giemsa method. The smear was first photographed and the location of individual cells, identified by morphology, was recorded on a cell map. The smear was then bleached with 50% acid ethanol and absolute methanol, and re-stained by the Feulgen method in 0.05% pararosaniline Schiff's reagent (pH 2.3) at 7 degrees C for 10 min. Nuclear red fluorescence was observed and the intensity of this fluorescence was proportional to the amount of DNA after prior irradiation of smears with green light for 9 hr. The method is useful for measuring cell DNA content in heterogeneous cell populations when morphological cell identification is required.
D001853:Bone Marrow / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D004307:Dose-Response Relationship, Radiation; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D013050:Spectrometry, Fluorescence
D004273:DNA, Neoplasm