Construction of a representative cDNA library from mRNA isolated from mouse oocytes.
A representative cDNA library has been constructed from the small quantities of poly(A)+ RNA present in unfertilised mouse oocytes. The construction of this library has been achieved by use of cow pea mosaic virus RNA as a carrier during isolation of polyadenylated message and during subsequent cloning procedures. This approach may be applicable to any system in which amounts of mRNA are limiting.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D051379:Mice / Q000235:genetics*; D009029:Mosaic Viruses / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA