Exudative, cellular and humoral reactions to platelet-activating factor (PAF-acether) in the pleural cavity of rats.
The reactions to platelet-activating factor (PAF-acether) injected into the pleural cavity of rats were compared with the reactions in animals injected with 0.9% NaCl. PAF-acether induced a maximum exudate after 30-60 min, which then decreased and disappeared after 24 h. The number of pleural leukocytes in the exudate was clearly decreased 30 min after the injection, was slightly increased after 6 h and was unchanged at other times. The estimation of lipid mediators in the pleural exudate obtained 30 and 60 min after the injection of PAF-acether revealed an increase in type-C4 leukotriene (LTC4) and a decrease in thromboxane B2 (TxB2) and in 6-keto prostaglandin F1 alpha (6-keto PGF1 alpha). In addition, the amount of histamine was found to be lower after 30 min. These results confirm in vivo that some biological effects of PAF-acether seem to involve the participation of other mediators.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D006632:Histamine / Q000378:metabolism; D007700:Kinetics; D007958:Leukocyte Count; D008297:Male; D010972:Platelet Activating Factor / Q000502:physiology*; D010996:Pleural Effusion / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism
D010972:Platelet Activating Factor; D013189:SRS-A; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D006632:Histamine