Stimulation by serotonin of immunoreactive peptide histidine isoleucine release from rat hypothalamus in vitro.
The effect of serotonin (5-HT) on the release of peptide histidine isoleucine-like immunoreactivity (PHI-LI) from rat hypothalamus was investigated in vitro with a perifusion system. A high potassium concentration (56 mM) stimulated PHI-LI release in a calcium-dependent manner. PHI-LI release was dose-relatedly stimulated by 5-HT (10(-6), 10(-5) and 10(-4) M). PHI-LI release induced by 5-HT (10(-5) M) was abolished by cyproheptadine (10(-4) M), a 5-HT antagonist. These results suggest that 5-HT has a stimulating effect on PHI release from the hypothalamus.
D000818:Animals; D002118:Calcium / Q000502:physiology; D003533:Cyproheptadine / Q000494:pharmacology; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D010451:Peptide PHI; D010455:Peptides / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000494:pharmacology* / Q000502:physiology; D013574:Synaptosomes / Q000378:metabolism
D010451:Peptide PHI; D010455:Peptides; D003533:Cyproheptadine; D012701:Serotonin; D011188:Potassium; D002118:Calcium