title: The effect of macromolecular conjugates of daunorubicin on nuclear ultrastructure in Trypanosoma brucei rhodesiense.
abstract: The effects of free and conjugated daunorubicin on T.b. rhodesiense in vitro are described. Free drug caused nucleolar lesions ranging from segregation to complete fragmentation. At equimolar concentrations a soluble bovine serum albumin conjugate with a stable succinyl linkage (D-BSAS) produced no ultrastructural lesions whereas a conjugate with a labile glutaraldehyde linkage (D-BSAG) and a conjugate linked to large agarose beads (D-ag) produced similar though less severe lesions than free drug. Polyisobutylcyanoacrylate nanoparticles caused trypanosomal lysis both with (D-PICA) and without adsorbed daunorubicin.
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000187:drug effects / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure*; D003487:Cyanoacrylates / Q000494:pharmacology*; D003630:Daunorubicin / Q000494:pharmacology*; D008854:Microscopy, Electron; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000648:ultrastructure*
chemical_list: D003487:Cyanoacrylates; D012710:Serum Albumin, Bovine; D003630:Daunorubicin