[Isolation of antibodies against soy protein from the eggs of immunized laying hens].
The authors report on their experiences with immunization of laying hens with alpha-, alpha'- and beta-conglycinin, and on the isolation of specific antibodies from the yolks. Activity and specificity of the immunoglobulins were tested by an enzyme-linked immuno sorbent assay (ELISA). This method of antibody production proved to be very convenient, especially in regard to the high yield of antibodies and the easy handling of the animals. The necessity of using specific antisera for quantitative immunoassays is pointed out.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002645:Chickens / Q000276:immunology*; D004531:Eggs / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D014674:Plant Proteins, Dietary / Q000276:immunology*; D030262:Soybean Proteins
D000906:Antibodies; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins
Gewinnung von Antikörpern gegen Sojaprotein aus Eiern immunisierter Legehennen.