Chemical mutations of the catalytic carboxyl groups in lysozyme to the corresponding amides.
In a two-step process, esterification and ammonolysis, Glu-35 and Asp-52 in lysozyme were amidated to glutamine and asparagine residues. Since the side chains of glutamine and asparagine are almost equal in size to those of glutamic acid and aspartic acid, these conversions would provide appropriate derivatives to elucidate the catalytic participations of these residues. The enzymatic activities of the resulting [Gln35]lysozyme and [Asn52]lysozyme were found to be less than 4% of that of native lysozyme in a pH range of 3.4-8.0. As these derivatives were inactive, we could determine the dissociation constants (Ks values) for the binding of beta-1,4-linked n-mer, a hexasaccharide of N-acetyl-D-glucosamine, to [Gln35]lysozyme and [Asn52] lysozyme. The values of Ks at pH 5.5 and 40 degrees C were 1.6 X 10(-5) M for [Gln35]lysozyme and 2.7 X 10(-5) M for [Asn52]lysozyme. These values are similar to that for native lysozyme. The results are direct proof for the involvements of Glu35 and Asp52 in the catalytic action of lysozyme. A method for ammonolysis of ester groups in proteins in liquid ammonia is described and will be useful for amidation of carboxyl groups of proteins.
D000596:Amino Acids / Q000032:analysis; D001216:Asparagine; D001224:Aspartic Acid; D002846:Chromatography, Affinity; D005971:Glutamates; D005973:Glutamine; D007700:Kinetics; D009113:Muramidase / Q000302:isolation & purification / Q000378:metabolism*; D009154:Mutation; D013449:Sulfonamides / Q000494:pharmacology; D000096926:Benzenesulfonamides
D000596:Amino Acids; D001216:Asparagine; D001224:Aspartic Acid; D005971:Glutamates; D005973:Glutamine; D009113:Muramidase; D013449:Sulfonamides; C050157:4-diazomethyl-N,N-dimethylbenzenesulfonamide; D000096926:Benzenesulfonamides