title: Immunological activation of rainbow trout macrophages induced in vitro by sperm autoantibodies and factors derived from testis sensitised leucocytes.
abstract: Young cultures of rainbow trout macrophages were found to significantly increase in diameter following addition of muramyl dipeptide or of supernatants from testis/FCA sensitised leucocytes incubated with spermatozoa, or a testis extract. However, such supernatants caused little migration inhibition or increase in phagocytosis of spermatozoa by macrophages, whereas opsonization of spermatozoa with anti-sperm serum caused an almost six-fold increase in phagocytosis. These results suggest an interplay between humoral and cell-mediated immune reactions upon macrophages during experimental autoimmunity to the testis in trout.
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000502:physiology*; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D007962:Leukocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D010587:Phagocytosis; D012487:Salmonidae / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*; D013737:Testis / Q000276:immunology; D014337:Trout / Q000276:immunology*
chemical_list: D001323:Autoantibodies