Sensitive high-performance liquid chromatographic determination of pirenzepine in plasma.
A high-performance liquid chromatographic method for the determination of pirenzepine in human plasma is reported using imipramine as an internal standard. The assay has a lower limit of detection of 2.5 ng/ml. The calibration function is found to be linear in the range from 5 ng/ml up to at least 100 ng/ml. Two sets of chromatographic conditions are described, which provide different chromatographic selectivities for the separation of the compounds of interest from other material present in a sample.
D001570:Benzodiazepinones / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010890:Pirenzepine
D001570:Benzodiazepinones; D010890:Pirenzepine