Modifications in the histochemical and biochemical changes in tenotomized rat soleus by denervation.
Response of tenotomized rat soleus muscle to denervation performed at different time intervals, has been investigated. Tenotomized muscles showed typical central core lesions seven days post-operatively. These were not observed in m-ATPase stained sections of simultaneously denervated and tenotomized muscles, and muscles denervated 24 h after tenotomy. Central core lesions were not prevented in muscles denervated 28 h after tenotomy, indicating that tenotomy effects responsible for central lesions are completed by this time. Myosin light chain pattern of muscles denervated 28 h after tenotomy, and tenotomized only were similar showing increased LC3/LC1 ratio. Simultaneously denervated and tenotomized muscle however showed all the three light chains relatively equal in quantity. The results suggest that elimination of neural activation within 28 h prevents myofibrillar loss and minimized other changes which occur due to tenotomy.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006651:Histocytochemistry; D008297:Male; D009121:Muscle Denervation; D009132:Muscles / Q000378:metabolism* / Q000473:pathology; D009218:Myosins / Q000378:metabolism; D051381:Rats; D013710:Tendons / Q000502:physiology
D009218:Myosins