[Effect of N-nitrosodimethylamine and precursors of this carcinogen on rat kidney cells in monolayer culture].
Kidney tissue culture of 3-day old rats was cultivated in the presence of carcinogenic N-nitrosodimethylamine (NDMA), sodium nitrite, amidopyrine and a combination of these substances forming NDMA. All these substances induce changes in the number of DNA-synthesizing cells, monolayer density, percentage ratio of epithelial and fibroblastoid cells in the kidney cultures. The above changes are typical of each of the tested components, that permits using one-layer culture of the rat kidneys as a model for investigating cell response to the precursors of NDMA and to this carcinogen.
D000632:Aminopyrine; D000818:Animals; D002273:Carcinogens; D004128:Dimethylnitrosamine; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects*; D009153:Mutagens; D051381:Rats; D012977:Sodium Nitrite
D002273:Carcinogens; D009153:Mutagens; D000632:Aminopyrine; D012977:Sodium Nitrite; D004128:Dimethylnitrosamine
Deĭstvie N-nitrozodimetilamina i predshestvennikov étogo kantserogena na kletki pochek krys v odnosloĭnoĭ kul'ture.