title: In vivo stability of in vitro labelled 99Tcm-red blood cells for radionuclide determination of left ventricular ejection fractions and volumes.
abstract: Serial determination of radionuclide left ventricular ejection fraction and volumes require a label that remains highly stable after introduction into the human circulation. We have evaluated the in vivo stability of 99Tcm-red blood cells (99Tcm-RBC) labelled in vitro by preparation with small amounts of a stannous agent in 19 patients with coronary artery disease. The distribution volume of 99Tcm-RBC was virtually identical to that of RBC labelled with 51Cr. Labelling efficiency expressed as the cell bound/total activity was more than 95% and remained high throughout 1 h after injection of the label. The effective in vivo half-times (T1/2) calculated from venous blood activity and externally recorded left ventricular end-diastolic frame activity were 342 +/- 103 min (mean +/- S.D.) and 306 +/- 92 min, respectively. A significant correlation, r = 0.86 (p less than 0.01) was found between the T1/2 values calculated from the two methods. Thus, a high in vivo stability of the label was demonstrated with considerable inter-patient variation. The labelling procedure seems suitable for serial performance of radionuclide cardiography within an hour after injection of 99Tcm-RBC. However, serial volume determination necessitates individual calculation of in vivo tracer decay from either venous blood or externally recorded activity in the left ventricular area during steady conditions.
mesh_terms: D000368:Aged; D003327:Coronary Disease / Q000000981:diagnostic imaging*; D004912:Erythrocytes; D006207:Half-Life; D006801:Humans; D007553:Isotope Labeling; D008875:Middle Aged; D011877:Radionuclide Imaging; D013318:Stroke Volume; D013669:Technetium Tc 99m Medronate; D013997:Time Factors
chemical_list: D013669:Technetium Tc 99m Medronate