Methotrexate increases glycogenolysis in the intact rat liver.
The effect of methotrexate, an anti-cancer drug, on carbohydrate metabolism in the isolated perfused rat liver was investigated. Glucose release from endogenous glycogen (glycogenolysis) is strongly activated by methotrexate. The phenomenon shows a well defined concentration dependence: 100% activation occurs at 2.2 X 10(-4) M. Glycolysis and oxygen consumption are only slightly affected.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000008:administration & dosage / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains / Q000145:classification
D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D019289:Pyruvic Acid; D006003:Glycogen; D005947:Glucose; D008727:Methotrexate