title: Experimental evidence for the role of lipids in adherence of Candida spp. to human buccal epithelial cells.
abstract: Lipids extracted from Candida albicans and C. tropicalis, but not from the weakly adherent C. pseudotropicalis, significantly blocked in vitro adherence of the respective yeast cells to buccal epithelial cells. The percentage of reduction from control values ranged between 16.4 and 42.1%, depending on the species, the strain, and the solvent used for lipid extraction. The constituent lipid classes of both the acetone and chloroform-methanol extracts of C. albicans ATCC 10231 were qualitatively and quantitatively analyzed. The individual classes were isolated by preparative thin-layer chromatography and then tested for their effects on the adherence of this strain to buccal epithelial cells. Individual phospholipids, sterols, and steryl esters blocked adherence significantly (between 15.5 and 55.7% reduction). Triacylglycerols and free fatty acids showed no effect whatsoever. The same results were obtained when standard lipid samples were investigated.
mesh_terms: D000268:Adhesiveness; D002175:Candida / Q000472:pathogenicity*; D002241:Carbohydrates / Q000502:physiology; D004848:Epithelium / Q000382:microbiology; D006801:Humans; D008055:Lipids / Q000145:classification / Q000502:physiology*; D009061:Mouth Mucosa / Q000382:microbiology*
chemical_list: D002241:Carbohydrates; D008055:Lipids