Robotic sample preparation evaluated for the immunochemical determination of cardiac isoenzymes.
A general-purpose bench-top laboratory robot was programmed to perform sample preparation for immunoprecipitative and immunoinhibitory determinations of cardiac isoenzymes with commercial kits. Run size could be varied from 1 to 24 patients' samples, and was determined by the robot without prompting. The robot placed the processed samples into a sample rotor for dried transfer to a batch centrifugal analyzer. Robotic precision compared well with that of manual performance of the same procedures at four concentrations of lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 activity and at one of two concentrations of creatine kinase (EC 2.7.3.2.) isoenzyme MB activity; for the other creatine kinase MB concentration, use of the robot significantly improved precision. Results for 100 samples from patients after open-heart surgery were highly correlated with manually obtained results for both isoenzyme determinations (r less that 0.96 each). Time to completion for small run sizes for either approach was comparable about 25 min. For larger batches, robotic run time increased rapidly, to 169 min for 24 patients' samples vs 41 min by manual assay.
D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D001331:Automation; D001800:Blood Specimen Collection / Q000295:instrumentation*; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D009206:Myocardium / Q000201:enzymology*; D011933:Reagent Kits, Diagnostic; D012984:Software
D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase