[Effect of pH on the regulatory characteristics of energy metabolism in human erythrocytes].
The glucose consumption rate versus ATP content in human red cells (regulatory patterns of glycolysis) and ATP concentration versus glucose uptake rate in red cell suspension (regulatory patterns of total ATPases), when the rate of glucose uptake is constant and lower than the rate of glucose consumption at physiological conditions, were measured at different pH values. The shape of both types of kinetic curves was found to be dependent on the pH of the incubation medium but the same for the red cells taken from different donors. It is supposed that at alkaline pH, glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase reactions become the rate-limiting steps of glycolysis instead of hexokinase and phosphofructokinase under physiological conditions.
D000255:Adenosine Triphosphate / Q000097:blood / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D004734:Energy Metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007773:Lactates / Q000097:blood / Q000378:metabolism
D001786:Blood Glucose; D007773:Lactates; D000255:Adenosine Triphosphate
Vliianie pH na reguliatornye kharakteristiki énergeticheskogo metabolizma éritrotsitov cheloveka.