Pyronin G as a fluorescent probe for quantitative determination of the membrane potential of mitochondria.
Added to mitochondrial suspension, pyronin G changes the intensity of its fluorescence depending on the membrane potential (energy state) of the mitochondria. The mechanism of this effect is studied and a dependence is obtained between the membrane potential and the fluorescence intensity. This permits quantitative determination of the membrane potential by the changes in the fluorescence of the suspension. A method is proposed for measuring the membrane potential of vesicles in the -120 to -220 mV interval.
D000818:Animals; D007700:Kinetics; D008433:Mathematics; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption; D011048:Polarography; D011754:Pyronine; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods; D014966:Xanthenes
D014966:Xanthenes; D011754:Pyronine