title: Pig heart fumarase really does exhibit negative kinetic co-operativity at a constant ionic strength.
abstract: The kinetics of the action of fumarase on L-malate and fumarate were investigated at constant ionic strength. This was done to evaluate reports that fumarase follows simple Michaelis-Menten kinetics. However, when pH, buffer concentration and ionic strength are all maintained at constant values, the Lineweaver-Burk plots exhibit pronounced downward curvature, characteristic of negative kinetic co-operativity.
mesh_terms: D000818:Animals; D005649:Fumarate Hydratase / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D007700:Kinetics; D008293:Malates / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology*; D009994:Osmolar Concentration; D013552:Swine
chemical_list: D005650:Fumarates; D008293:Malates; C030298:malic acid; C032005:fumaric acid; D005649:Fumarate Hydratase