Transbilayer migration (flip-flop) of 12-(4-azido-2-nitrophenoxy)stearoyl glucosamine in large unilamellar phospholipid vesicles.
The ability of the glycolipid photoprobe, 12-(4-azido-2-nitrophenoxy)-stearoyl[1-14C]glucosamine (12-APS-GlcN), to undergo transbilayer flip-flop and intermembrane transfer between liposomes was examined. It was found that probe which was incorporated into membranes during the preparation of large unilamellar vesicles (LUVs) could be rapidly and completely extracted by incubation of these donor vesicles (in the liquid-crystalline state) with probe-free acceptor vesicles.
D001386:Azides; D008051:Lipid Bilayers; D008954:Models, Biological; D010714:Phosphatidylethanolamines; D011663:Pulmonary Surfactants
D001386:Azides; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D011663:Pulmonary Surfactants; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C019266:12-(4-azido-2-nitrophenoxy)stearoylglucosamine