The effect of estradiol on enzymatic activity and androgen secretion in Leydig cells in vitro.
The effect of estradiol-17 beta (E2) on cultured Leydig cells isolated from mice testes was studied histochemically and radioimmunologically. Both analyses showed low activity of the enzymes studied and a decrease of the androgen level in E2 treated cultures. When the dose of estradiol preceded LH addition the androgen response to LH was attenuated. These results suggest direct inhibition of Leydig cell function by exogenous estradiol.
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000201:enzymology* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D051379:Mice; D011863:Radioimmunoassay
D000728:Androgens; D004958:Estradiol; D007986:Luteinizing Hormone