Chymotrypsin catalysed synthesis of fluorescent amino acid amide and peptide derivatives.
Chymotrypsin catalyses a condensation reaction between 1-methyl-3,4-dihydro-beta-carboline-3-methyl carboxylate and amino acid amides or peptides, yielding fluorescent derivatives. During the peptide bond formation, the enzyme ensures the reaction's steric control of both carboxyl and amino components.
D000577:Amides; D000818:Animals; D002243:Carbolines; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism*; D005456:Fluorescent Dyes; D007700:Kinetics; D009842:Oligopeptides / Q000138:chemical synthesis; D010179:Pancreas / Q000201:enzymology; D013050:Spectrometry, Fluorescence
D000577:Amides; D002243:Carbolines; D005456:Fluorescent Dyes; D009842:Oligopeptides; C008431:1-methyl-3,4-dihydro-beta-carboline-3-carboxylic acid; D002918:Chymotrypsin