Attempts to improve tissue survival during ex vivo storage.
Ex vivo hypothermic perfusion has been shown to enhance short-term survival of organs before transplantation. The effects of perfusion, control of media pH, and systemic drug treatment were studied utilizing superficial epigastric free flaps in Sprague-Dawley rats. Viability of the flaps could be reliably maintained (9/10, 90%) for 72 hours using simple storage in phosphate-buffered Ringer's (pH 7.8) at 4 degrees C. Pretreatment with prostaglandin E1 was of slight benefit. Flap perfusion with or without pharmacologic agents was not beneficial.
D000818:Animals; D003080:Cold Temperature; D003470:Culture Media; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013524:Surgical Flaps; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival
D003470:Culture Media