Undulating course of nerve fibres and bands of Fontana in peripheral nerves of the rat.
The undulating course of nerve fibres and the optical effect of that course, i.e. the bands of Fontana, were studied in the peripheral nerves of the adult rat using light microscopy. The arrangement of collagen fibres in the endoneurium of these nerves was evaluated using transmission and scanning electron microscopy. No nerve fibres undulation was noted on the intracranial sections of the cranial nerves or on the spinal roots. In their endoneurium a few, irregularly arranged collagen fibrils were found. In contrast, the nerve fibres undulation and Fontana's bands were a constant feature in the peripheral course of the nerve trunks. They were discernible in vivo and on excised unfixed as well as fixed nerves. The nerve fibres follow a sine-curve course of variable frequency and amplitude. Exposed in vivo, the nerve fibres retained their wave-like course even after removal of the epineurium and perineurium. The endoneurium of these nerves contained numerous undulating longitudinally oriented bundles of collagen fibrils. These findings suggest that the undulating course of the nerve fibres in peripheral nerves is conditional upon the quantity and arrangement of their endoneurial collagen fibrils. When the nerve was stretched in the course of movement, the undulation became straightened out until it disappeared. Conversely, nerve shortening enhanced the undulation. Thus the wave-like alignment of the nerve fibres represents a physiological reserve length for nerve stretching.
D000818:Animals; D003391:Cranial Nerves / Q000166:cytology / Q000648:ultrastructure; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology / Q000648:ultrastructure; D010525:Peripheral Nerves / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains