title: Breath hydrogen concentration and small intestinal malabsorption in calves.
abstract: Breath hydrogen concentrations were measured to assess intestinal carbohydrate malabsorption in preruminating calves. Oral administration of 1.25 g of lactulose (a nonabsorbable carbohydrate)/kg to calves produced breath hydrogen concentrations significantly (P less than 0.001) higher than values determined after calves were fed milk and before the treatment was given. This indicates that, in the calf, fermentation of nonabsorbed carbohydrates results in increased breath hydrogen values. To induce small intestinal malabsorption, chloramphenicol was administered orally at 50 mg/kg, 2 times a day, to 5 calves for 3 days. Before therapy was started, each calf was fitted with a duodenal cannula to facilitate collection of intestinal mucosal biopsy samples during treatment. Chloramphenicol therapy significantly (P less than 0.001) increased breath hydrogen concentrations from those values measured after calves were fed milk alone. Concurrently, chloramphenicol administration significantly decreased intestinal villous length (P less than 0.001) and D-xylose absorption (P less than 0.05), compared with those values before treatment was given. These results demonstrate that decreased intestinal absorptive capacity is associated with an increase in breath hydrogen concentrations and that breath hydrogen may be useful in evaluating malabsorption in calves with naturally occurring enteric disease.
mesh_terms: D000818:Animals; D001944:Breath Tests; D050260:Carbohydrate Metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000503:physiopathology; D006859:Hydrogen / Q000032:analysis*; D008286:Malabsorption Syndromes / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D008297:Male
chemical_list: D006859:Hydrogen