[Effect of cyclosporin A, dexamethasone and various preservatives on epithelial wound healing in the denervated guinea pig cornea].
In order to examine a possible connection between punctate keratopathy following keratoplasty and the various drugs used in postoperative treatment, denervation was performed in white Pirbright guinea pigs by corneal trepanation with a 6 mm trephine to a depth of 0.25 mm. One week later an iodine gas applicator was used to produce erosion in the cornea, which had healed in the meantime. Until the erosions healed, the corneas were photographed four times daily after fluorescein staining; the negatives were enlarged by projection and the erosions measured planimetrically. The linear regression method was used to calculate a compensating straight line and thus the hourly epithelial wound-healing surface (mm2/h) for each eye. This was 0.76 mm2/h in control eyes. Dexamethasone 0.1%, benzalconium chloride 0.01%, and chlorobutanol 0.2% did not significantly inhibit wound healing, while Spersadex (dexamethasone 0.1% + benzalconium chloride 0.01%) and Totocortin (dexamethasone 0.1 + chlorobutanol 0.2%) did. Polyvinyl alcohol (1.4% without preservative) significantly delayed epithelial wound healing. Cyclosporin A 2% (in castor oil) had no influence on epithelial wound healing.
D000818:Animals; D001548:Benzalkonium Compounds / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002724:Chlorobutanol / Q000494:pharmacology; D003315:Cornea / Q000187:drug effects* / Q000294:innervation; D003524:Cyclosporins / Q000494:pharmacology*; D003714:Denervation; D003907:Dexamethasone / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects; D006168:Guinea Pigs; D009926:Organ Preservation; D014945:Wound Healing / Q000187:drug effects*
D001548:Benzalkonium Compounds; D003524:Cyclosporins; D003907:Dexamethasone; D002724:Chlorobutanol
Der Einfluss von Cyclosporin A, Dexamethason und verschiedenen Knoservierungsmitteln auf die epitheliale Wundheilung der denervierten Meerschweinchenhornhaut.