title: Electron microscopic immunocytochemistry. Silver enhancement of colloidal gold marker allows double labeling with the same primary antibody.
abstract: Electron microscopic sections, immunocytochemically labeled with colloidal gold, can be prepared for double labeling by applying the "EM-silver enhancement" procedure. This method, a photographic, so-called physical, development, increases the size of the gold marker to a predeterminable value and thereby inactivates the anti-species antibody present on the gold grain, thus allowing the labeling of a second antigen with antibody raised in the same species.
mesh_terms: D000911:Antibodies, Monoclonal; D006046:Gold; D007120:Immunochemistry / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D012834:Silver
chemical_list: D000911:Antibodies, Monoclonal; D012834:Silver; D006046:Gold