Effects of 6,6'-dithiodinicotinic acid (CPDS) and its metabolite 6-mercaptonicotinic acid (6-MNA) on murine and hamster fibroblasts (3T3 and BHK) and murine metastatic melanoma cells (F10).
We investigated the action of 6,6'-dithiodinicotinic acid (CPDS) and its metabolite 6-mercaptonicotinic acid (6-MNA) in vitro on murine (3T3) and baby hamster kidney (BHK) fibroblasts and an in vivo highly metastatic subline of murine B16 melanoma (F10). CPDS determined an inhibition of cell growth and a decrease in cell adhesion, while 6-MNA had no effect. When combined with data of the mitotic index and endogenous purine ribonucleotides (on which the drugs seem to have no effect), these observations are conceivable with the hypothesis that the primary target of CPDS is cell membrane.
D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D007680:Kidney Neoplasms / Q000378:metabolism / Q000648:ultrastructure; D008546:Melanoma, Experimental / Q000378:metabolism / Q000503:physiopathology* / Q000648:ultrastructure; D008940:Mitotic Index; D009539:Nicotinic Acids / Q000494:pharmacology*; D009711:Nucleotides / Q000032:analysis; D013936:Thymidine / Q000378:metabolism
D009539:Nicotinic Acids; D009711:Nucleotides; C001102:6,6'-dithiodinicotinic acid; C042826:6-mercapto-3-pyridinecarboxylic acid; D013936:Thymidine