[Host proteins in purified concentrations of the influenza virus].
A comparative study of three methods for purification and concentration of influenza virus (adsorption on and elution from formalin-treated erythrocytes, sorption method, and purification on nuclear filters) demonstrated a significant decreased in ovalbumin content. By this criterion, all the three methods of preliminary purification yield the final preparation with a similar ovalbumin content. A more detailed study of the protein composition of influenza virus concentrates showed purification by elution from formalin-treated erythrocytes to remove greated amounts of protein admixtures. Electrophoregrams of virus concentrates produced by the adsorption method using macropore glass 8000 revealed a protein which passed into the virus suspension in sufficiently large amounts. This protein was identified as conalbumin.
D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification; D004912:Erythrocytes; D005069:Evaluation Studies as Topic; D009980:Influenza A virus / Q000302:isolation & purification*; D007252:Influenza Vaccines / Q000032:analysis / Q000302:isolation & purification; D008722:Methods; D010047:Ovalbumin / Q000032:analysis / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D014613:Vaccines, Attenuated / Q000032:analysis / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification*
D001798:Blood Proteins; D007252:Influenza Vaccines; D011506:Proteins; D014613:Vaccines, Attenuated; D014764:Viral Proteins; D010047:Ovalbumin
Izuchenie belkov khoziaina v ochishchennykh kontsentratsiiakh virusa.