Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens.
A method for the clonal analysis of antibodies against high M.Wt. antigens is described. It involves isoelectric focusing, electrophoretic transfer of the focused antibodies to a nitrocellulose membrane and detection of the membrane-bound antibodies by overlay with radiolabelled antigen (reverse immunoblotting). The application of this technique to the clonal analysis of autoantibodies against thyroglobulin and DNA is described.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D003101:Collodion; D004247:DNA / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010209:Paper; D013954:Thyroglobulin / Q000276:immunology
D000941:Antigens; D001323:Autoantibodies; D007457:Iodine Radioisotopes; D003101:Collodion; D004247:DNA; D013954:Thyroglobulin