Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis.
The enzyme-linked immunosorbent assay was standardized for the search for specific antibodies in human neurocysticercosis. A crude cysticercal extract and two partially purified antigenic fractions were used, as well as serum and cerebrospinal fluid (CSF) samples of different groups of subjects. Immunoglobulin G (IgG) antibodies were detected in serum and CSF, with a sensitivity of 85 and 90%, respectively. Specificity was 96% with a partially purified antigen and 100% with the crude cysticercal extract. IgM and IgA antibodies were detected less frequently, and IgE was detected only occasionally, both in serum and CSF. Analysis of serum and CSF samples drawn from the same patient did not always reveal the presence of anticysticercus antibodies in both samples. A significant correlation was found between the presence or absence of IgG antibodies in the CSF and the morphological appearance of the parasite (undamaged or calcified). Variations in the humoral response were not found to correlate with clinical and laboratory findings.
D000293:Adolescent; D000328:Adult; D000947:Antigens, Helminth / Q000276:immunology; D002648:Child; D003551:Cysticercosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000134:cerebrospinal fluid; D007073:Immunoglobulin E / Q000032:analysis / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000032:analysis / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000032:analysis / Q000134:cerebrospinal fluid; D007136:Immunoglobulins / Q000032:analysis* / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D009422:Nervous System Diseases / Q000175:diagnosis / Q000276:immunology*; D013621:Taenia / Q000276:immunology*
D000947:Antigens, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007073:Immunoglobulin E