Fractionation of bone-marrow cells by counter-current distribution in aqueous polymer two-phase systems. Relation between settling time and the efficiency of separation.
The fractionation of heterogeneous populations of rat and human bone-marrow cells has been studied by counter-current distribution in a charged 5% dextran-4% poly(ethylene glycol) two-phase system. The subfractionation into two broad populations has been achieved at a low top/bottom phase volume ratio by increasing (up to 20 min) the settling time allowed for the phases to be separated after each mixing step. No effect of this parameter on a homogeneous population of erythrocytes has been observed. However, heterogeneous cell separations can be improved by exploiting different phase settling times.
D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation; D003377:Countercurrent Distribution; D004912:Erythrocytes / Q000166:cytology; D007504:Iron Radioisotopes; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D007504:Iron Radioisotopes