title: Slow biliary elimination of methyl mercury in the marine elasmobranchs, Raja erinacea and Squalus acanthias.
abstract: The present study examined the ability of two marine elasmobranchs (Raja erinacea, little skate, and Squalus acanthias, spiny dogfish shark) to excrete methyl mercury into bile, a major excretory route in mammals. 203Hg-labeled methyl mercury chloride was administered via the caudal vein, and bile collected through exteriorized cannulas in the free swimming fish. Skates and dogfish sharks excreted only a small fraction of the 203Hg into bile over a 3-day period: in the skate, the 3-day cumulative excretion (as a % of dose) was 0.44 +/- 0.10 (n = 4, +/- SD), 0.71 +/- 0.23 (n = 6), and 1.00 +/- 0.34(n = 4) for doses of 1, 5, and 20 mumol/kg, respectively, while the shark excreted only 0.15 +/- 0.15% (n = 8) at a dose of 5 mumol/kg. As in mammals, the availability of hepatic and biliary glutathione was a determinant of the biliary excretion of methyl mercury in these species: the administration of sulfobromophthalein, a compound known to inhibit both glutathione and methyl mercury excretion in rats, or of L-buthionine-S,R-sulfoximine, an inhibitor of glutathione biosynthesis, decreased the biliary excretion of both glutathione and mercury in the skate. The slow hepatic excretory process for methyl mercury in the skate and shark was attributed to an inordinately slow rate of bile formation: from 1 to 4 ml/kg X day. An inefficient biliary excretory process in fish may account in part for the long biological half-times for methyl mercury in marine species.
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D019328:Buthionine Sulfoximine; D004284:Dogfish / Q000378:metabolism*; D004555:Electric Fish / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D008767:Methylmercury Compounds / Q000378:metabolism*; D012754:Sharks / Q000378:metabolism*; D012860:Skates, Fish / Q000378:metabolism*; D013448:Sulfobromophthalein / Q000494:pharmacology; D013997:Time Factors
chemical_list: D008767:Methylmercury Compounds; D013448:Sulfobromophthalein; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D005978:Glutathione