Oversulfated chondroitin sulfate proteoglycan in cultured human peritoneal macrophages.
Human peritoneal macrophages were cultured in vitro and labeled with [35S]-sulfate. Both on day 1 and day 6 in culture the cells were found to synthesize exclusively chondroitin sulfate proteoglycan, the main part (70%) being associated with the medium after a 20 hour pulse. The glycosaminoglycan chains were found to be oversulfated both after 1 and 6 days in culture, due to the presence of disulfated disaccharide units.
D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008970:Molecular Weight; D011509:Proteoglycans / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*
D011508:Chondroitin Sulfate Proteoglycans; D011509:Proteoglycans; D013431:Sulfates