Determination of benzylpenicillin and probenecid in human body fluids by high-performance liquid chromatography.
A method for the determination of benzylpenicillin and probenecid concentrations in human body fluids using ion-pair reversed-phase chromatography with UV detection has been developed. For plasma samples two extraction techniques were investigated. Precipitation of the plasma proteins with acetonitrile followed by liquid-liquid extraction offered the best results. The limits of detection were 0.5 microgram/ml for benzylpenicillin and 0.25 microgram/ml for probenecid, which offer sufficient sensitivity for application in pharmacokinetic experiments.
D001826:Body Fluids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010400:Penicillin G / Q000032:analysis*; D011339:Probenecid / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D011339:Probenecid; D010400:Penicillin G