Increased formation and keeping of ribosomes during dietary recovery of mouse kidney.
In the kidney of 5-day protein-depleted mice there is a decrease of 23% in the rRNA mass. When these animals are fed with a complete diet, rRNA content is restored to its normal value after 24 h of refeeding. The mechanisms that underlie this phenomenon were studied. It was found that the activity of rRNA polymerase I in the nuclei of kidneys from refed mice showed an increase of about twofold compared with the activity in normal and protein-depleted nuclei. The in vivo incorporation of a large dose (nontrace) of [14C]orotic acid into rRNA was also twofold enhanced in kidneys from refed mice. Ribosome degradation (measured by the disappearance of radioactivity from either ribosomal proteins or rRNA previously labeled by the injection of NaH14CO3 and [14C]orotic acid to the mice, respectively) stopped during the 1st day after refeeding. The estimation of the difference between the rRNA synthesis rate and the net rRNA increase also demonstrated a decrease in the rRNA degradation rate in refed mice.
D000818:Animals; D004247:DNA / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D009963:Orotic Acid / Q000378:metabolism; D011488:Protein Deficiency / Q000178:diet therapy* / Q000378:metabolism; D012318:RNA Polymerase I / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D009963:Orotic Acid; D004247:DNA; D012318:RNA Polymerase I