title: A versatile low-cost apparatus for cell electrofusion and other electrophysiological treatments.
abstract: Instrumentation is described which will generate AC fields and DC square pulses for cell electrofusion, electroporation and other electrophysiological experiments. The equipment can generate a constant or intermittent AC field and a single square pulse, either alone or in combination. It can also generate a train of intermittent square pulses. The components for the instrumentation are commercially available and can be assembled by persons with little or no training in electronics. A design is given for a mass treatment chamber that can be easily constructed and viewed with a microscope.
mesh_terms: D002468:Cell Physiological Phenomena; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D018521:Plant Physiological Phenomena; D011523:Protoplasts / Q000502:physiology