Albumin binding of bumetanide.
Bumetanide binding to human serum albumin was studied using ultrafiltration. The first stoichiometric binding constant for bumetanide is 6.4 X 10(4) M-1. Bumetanide competes with bilirubin for human serum albumin binding, having a KDispl (displacement constant) of 6.2 X 10(3) M-1 measured by the peroxidase method. This displacement effect is also observed using pooled umbilical cord serum and pooled adult serum employing a dialysis rate method. Bumetanide competes to a lesser degree with diazepam binding to human serum albumin. No competition with diazepam occurs using umbilical cord or adult serum. Pharmacologic concentrations of bumetanide would not significantly affect bilirubin-albumin binding and should not increase the risk of bilirubin encephalopathy in newborn infants.
D000328:Adult; D001667:Binding, Competitive; D002034:Bumetanide / Q000097:blood*; D003622:Dapsone / Q000031:analogs & derivatives / Q000097:blood; D003975:Diazepam / Q000097:blood; D004232:Diuretics / Q000097:blood*; D005312:Fetal Blood; D005665:Furosemide / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007700:Kinetics; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*
D004232:Diuretics; D012709:Serum Albumin; D002034:Bumetanide; D005665:Furosemide; D003622:Dapsone; C018965:monoacetyldapsone; D003975:Diazepam