A high-performance liquid chromatographic method for the determination of dolichyl phosphates in tissues.
A sensitive high-performance liquid chromatographic method has been developed for the determination of dolichyl phosphate homologs in tissues. Dolichyl phosphates were methylated and derivatized with 3-(9-anthryl)-diazo-2-propene to yield methyl 3-(9-anthryl)-2-propenyl dolichyl phosphates. As an internal standard, 2,2-didecaprenylethyl phosphate was used. The derivatives were separated on a reversed-phase column using isocratic elution, and detected fluorometrically. The method with fluorescence detection was sufficiently sensitive to measure the concentration of dolichyl phosphate homologs in tissues.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003640:Dealkylation; D004288:Dolichol Phosphates / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry; D011106:Polyisoprenyl Phosphates / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D004288:Dolichol Phosphates; D011106:Polyisoprenyl Phosphates