Relaxin treatment alters the kinetic properties of myosin light chain kinase activity in rat myometrial cells in culture.
Relaxin treatment altered the kinetic properties of rat myometrial cell myosin light chain kinase (MLCK) by increasing the K50 of the enzyme for calmodulin (CaM) from 1.1 +/- 0.1 to 38 +/- 14 nM. When MLCK was assayed in the presence of 7 nM CaM to maximize the effect of the decreased affinity for CaM between control and relaxin-treated groups, a rapid concentration-dependent effect of the hormone was observed. Relaxin decreased MLCK activity significantly within 1 min. The ED50 of the effect was 0.4 microgram/ml. In addition to its effect on Ca2+-CaM-dependent activity, relaxin also decreased Ca2+-CaM-independent MLCK activity. This decrease was not attributable to a decrease in the affinity of the enzyme for myosin. There was a temporal association between the effects of relaxin on mean cell length, elevation of cAMP levels in the presence of 0.4 microM forskolin previously shown in other studies, and the alteration of MLCK activity. All three parameters were changed significantly within 1 min after exposure to relaxin. The ED50 of relaxin for cell shape changes, cAMP elevation, and effects on MLCK activity were all approximately 0.4 microgram/ml. Relaxin may act in part by a cAMP-mediated phosphorylation of MLCK, thereby decreasing its affinity for CaM. The effect on MLCK may be linked to a decrease in the phosphorylation of myosin light chains and the promotion of uterine relaxation.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D007700:Kinetics; D009215:Myometrium / Q000187:drug effects / Q000201:enzymology*; D009219:Myosin-Light-Chain Kinase; D009218:Myosins / Q000378:metabolism; D009994:Osmolar Concentration; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012065:Relaxin / Q000494:pharmacology*
D002147:Calmodulin; D012065:Relaxin; D011494:Protein Kinases; D009219:Myosin-Light-Chain Kinase; D009218:Myosins; D002118:Calcium