Effect of decreased oxygen on in vitro release of endogenous 3,4-dihydroxyphenylethylamine from mouse striatum.
The effects of hypoxia on release of endogenous 3,4-dihydroxyphenylethylamine (DA, dopamine) were investigated in mouse striatal slices. Following a 60-min preincubation, potassium increased DA release 12 times between zero and 1 min. By 10 min, uptake processes exceeded release and DA levels in the media decreased. Hypoxia (low oxygen) and anoxia (no oxygen) increased DA in the media by 120 and 205%, respectively, but did not alter dihydroxyphenylacetic acid concentrations. Under similar conditions, anoxia increased [3H]DA uptake eight-fold. For the uptake studies, the amount of DA added to the media was critical; in the presence of high concentrations of DA, anoxia reduced reuptake. Regardless of exogenous DA, hypoxia and anoxia increased extracellular DA, which may play a role in ischemic cell damage.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003342:Corpus Striatum / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D002534:Hypoxia, Brain / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D011188:Potassium / Q000494:pharmacology
D015102:3,4-Dihydroxyphenylacetic Acid; D011188:Potassium; D002118:Calcium; D004298:Dopamine