A method for the fine-structural demonstration of lectin receptors.
A new two-step method using an Fc-fragment/ferritin conjugate as a marker for the visualization of lectin-binding sites on neuronal and other cell membranes is described. In this study of rat synaptosomes, three lectins were tested: concanavalin A, mistletoe lectin I and wheat germ agglutinin. The specificity of the method was proved by control experiments.
D000818:Animals; D003208:Concanavalin A / Q000378:metabolism; D005260:Female; D005293:Ferritins; D006651:Histocytochemistry; D007141:Immunoglobulin Fc Fragments; D037102:Lectins / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D028321:Plant Preparations; D010940:Plant Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D011975:Receptors, Mitogen / Q000032:analysis*; D054790:Ribosome Inactivating Proteins, Type 2; D013574:Synaptosomes / Q000378:metabolism / Q000648:ultrastructure; D014118:Toxins, Biological / Q000378:metabolism; D014909:Wheat Germ Agglutinins
D007141:Immunoglobulin Fc Fragments; D037102:Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D011975:Receptors, Mitogen; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; D014909:Wheat Germ Agglutinins; C009674:ribosome inactivating protein, Viscum; D003208:Concanavalin A; D005293:Ferritins