Androgen metabolism in the human epididymis. Effect of in vivo estrogen administration.
Androgen metabolism in human epididymis was studied by incubating tissue fragments with isotopically labeled testosterone (T) and androstenedione (A) under batch and superfusion conditions. Epididymides were obtained from 16 patients with prostatic cancer, 5 of them treated with diethylstilbestrol (2.5 mg/d) for several months prior to castration. Results from batch incubations with [3H]T (100 nM) for 2 h at 25 degrees C indicated a markedly lower 5 alpha-reductase activity in tissues from estrogen-treated patients, as evaluated by measuring the amounts of radioactive 5 alpha-dihydrotestosterone, 5 alpha-androstanediols and 5 alpha-androstanedione present in tissue and medium at the end of the incubation period. Superfusion experiments confirmed this estrogen effect and also showed a shift of the interconversion between A and T towards the reductive direction and a diminished tissue retention of DHT after estrogen treatment. These effects may contribute to the marked regression of the epididymal epithelium that was noted in the estrogen-treated patients, which is thought to be mainly the result of the inhibition of androgen biosynthesis caused by chemical hypophysectomy.
D000368:Aged; D000728:Androgens / Q000378:metabolism*; D000735:Androstenedione / Q000378:metabolism; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013739:Testosterone / Q000378:metabolism
D000728:Androgens; D004967:Estrogens; D013739:Testosterone; D000735:Androstenedione