title: Antibacterial immunity to Vibrio cholerae in rats.
abstract: Blind loops prepared in the small intestines of fasted, MgSO4-treated rats were shown to provide a simple, consistent and inexpensive means of studying mucosal colonisation by Vibrio cholerae serogroup O1. When c. 2000 cfu were injected, the number of mucosa-associated V. cholerae in each loop increased by c. 5-6 orders of magnitude in 10-14 h, without enterotoxin-induced fluid production. Scanning electronmicroscopy and culture suggested that most surface-associated organisms were present in the adherent surface mucus. V. cholerae strains varied in terms of surface-colonising capacity. Immunisation with V. cholerae given intra-intestinally greatly reduced the rate of increase and final number of mucosa-associated vibrios within the 14-h period after challenge. The method could be used to compare the immunity induced by various immunising regimens. Immunity was sometimes accompanied by intestinal mucus-borne antibody against V. cholerae lipopolysaccharide but was sometimes demonstrated in the absence of such antibody or of mucus-borne antibody to heat-sensitive surface protein.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D002771:Cholera / Q000276:immunology* / Q000382:microbiology; D005260:Female; D007114:Immunization; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology; D007421:Intestine, Small / Q000276:immunology / Q000382:microbiology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014734:Vibrio cholerae / Q000254:growth & development / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides