title: The behaviour of lung surfactant in electrolyte solutions.
abstract: Surface and electrokinetic properties of purified calf lung surfactant in various electrolyte solutions were determined. Surface properties were pH dependent in distilled water and the surfactant performed as a good lung surfactant only below pH 4. In more physiological media it was pH insensitive over the range 2-8.5. In distilled water at pH 6 its surface properties improved when NaCl was added up to 20 mM; above this concentration it had the surface properties required to stabilise alveoli. The surface properties of surfactant in distilled water were also restored by certain cations (Ca2+, Mg2+, Mn2+, Cd2+ and Ni2+) but not others (Na+, K+, La3+ and Fe3+) when added to an ionic strength of 5.6 mM. Cations that restored its surface activity also reduced the surface charge density on the surfactant particles. Aggregation of surfactant by various metal chlorides was studied by light scattering measurements and bore no relation to surface activity or the charge on the particles. Aggregation of surfactant particles by Ca2+, Cd2+ and Mn2+ was instantly reversed by addition of excess EGTA. The influence of electrolytes on the surface properties of lung surfactant is explained in terms of the electrostatic forces operating in the system.
mesh_terms: D002122:Calcium Chloride / Q000494:pharmacology; D004573:Electrolytes; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008670:Metals / Q000494:pharmacology; D009994:Osmolar Concentration; D011663:Pulmonary Surfactants / Q000032:analysis; D012965:Sodium Chloride / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D004573:Electrolytes; D008081:Liposomes; D008670:Metals; D011663:Pulmonary Surfactants; D012965:Sodium Chloride; D002122:Calcium Chloride