Vaccination of mice against malaria with soluble antigens. I. The effect of detergent, route of injection, and adjuvant.
Mice were vaccinated against lethal blood stage P. yoelii malaria with lysates of parasitised blood prepared in various detergents. Two injections, containing 25 micrograms of protein, of a Triton X-100 lysate gave optimal protection when injected intraperitoneally with saponin as adjuvant. Such lysates provide a starting point for the isolation of potential antigens for vaccination, selected for their protective activity in vivo.
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D003902:Detergents / Q000494:pharmacology; D005260:Female; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008288:Malaria / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010961:Plasmodium / Q000276:immunology*; D012503:Saponins / Q000494:pharmacology; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; D000953:Antigens, Protozoan; D003902:Detergents; D012503:Saponins; D014612:Vaccines