Human gamma gamma-enolase: two-site immunoradiometric assay with a single monoclonal antibody.
A monoclonal antibody (mAb), termed BBS/NC/VI-H14 (H14), that reacts with the human enzyme gamma gamma-enolase was prepared. It was directed against the gamma-subunit and did not cross-react with the alpha- or beta-subunit. The mAb H14 can be used for quantitative determination of gamma gamma-enolase in a two-site immunoradiometric assay (two-site IRMA). It is also suitable for immunostaining formalin-fixed tissues. The specific identification of gamma gamma-enolase provided by the two-site IRMA with H14 is discussed in relation to the cellular distribution of this protein.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001921:Brain / Q000201:enzymology*; D006168:Guinea Pigs; D006651:Histocytochemistry; D006801:Humans; D007118:Immunoassay; D007457:Iodine Radioisotopes; D007527:Isoenzymes / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000276:immunology; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000276:immunology
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D007527:Isoenzymes; D010751:Phosphopyruvate Hydratase