Involvement of leukocytes in the oxygenation and chlorination reaction of phenylbutazone.
A center carbon atom of 1,3-diketone moiety of phenylbutazone was oxidized to give three metabolites--4-hydroxyphenylbutazone (metabolite I), 4-hydroperoxyphenylbutazone (metabolite II) and 4-chlorophenylbutazone (metabolite III)--by the action of enzymes present in leukocyte extract obtained from peritoneal exudate of rats. Both metabolites II and III were produced by peroxidases, while metabolite I was produced by enzymes other than the peroxidases.
D000818:Animals; D066298:In Vitro Techniques; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D010653:Phenylbutazone / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship
D010544:Peroxidases; D010653:Phenylbutazone