[Changes in the activity of lactate dehydrogenase isoformes in mouse rhabdomyosarcoma under different proliferation conditions].
The activity of lactate dehydrogenase (LDH) isoenzymes was studied in clones of rhabdomyosarcoma (RMS) MX-53, being transplanted on mice of CC57W line. Clones obtained in lungs after the intravenous injection of a tumor cell suspension were transplanted into subcutaneous connective tissue (SCT) and eye anterior chamber (EAC). A study of LDH isoenzymes spectrum in transplants and native muscle tissues has been made using polyacrylamide gel electrophoresis and densitometry. We revealed a variability of LDH isoenzyme activity within one clone, and in population of clones under different proliferation conditions. Under all the proliferation conditions, we observed spectra characteristic of tumors of a given histogenesis with predominating activity of M-subunits. But during proliferation in EAC the M:H ratio decreased, and LDH spectra of tumor cells became more similar to the LDH spectrum of normal skeletal muscles. It is concluded that intra- and interclonal variations of the character "LDH isozyme spectrum" is strongly influenced by the environmental factors.
D000818:Animals; D000867:Anterior Chamber; D002999:Clone Cells / Q000201:enzymology; D005134:Eye Neoplasms / Q000201:enzymology; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008175:Lung Neoplasms / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D012208:Rhabdomyosarcoma / Q000201:enzymology*; D012878:Skin Neoplasms / Q000201:enzymology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Izmenenie aktivnosti izoform laktatdegidrogenazy v rabdomiosarkome myshi pri raznykh usloviiakh proliferatsii.