Effects of colon to antrum transposition on gastrin release and antral G-cell proliferation.
To investigate the effects of colonic mucosa on antral G-cell activity and proliferation, a colonic patch was transposed to the antrum. Thirty-nine rats were divided into 3 equal groups and underwent the following surgical procedures: Group 1: sham operation. Group 2: a standard portion (1 cm2) of the anterior surface of the antrum, including the pyloric sphincter, was removed and the defect was closed using a 10 mm segment of transverse colon shaped as a patch. Group 3 (control group): an identical procedure was carried out, closing the gastric defect with a gastro-duodenal suture. Three months after surgery, serum gastrin levels were determined by radioimmunoassay, both in fasting and freely fed animals. The antrum was then removed and examined for: counts of antral G-cell number by an immunoperoxidase method; evaluation of the cytoplasmic G-cell granule distribution (assumed as an index of gastrin turnover) by transmission electron microscopy investigation. The colon to antrum transposition significantly increased the antral G-cell density. By contrast, fasting serum gastrin levels and gastrin turnover were reduced after colon to antrum transposition, as compared with the relevant control procedure.
D000818:Animals; D002455:Cell Division; D003106:Colon / Q000166:cytology / Q000502:physiology*; D005755:Gastrins / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000502:physiology; D008297:Male; D011706:Pyloric Antrum / Q000166:cytology / Q000502:physiology*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D005755:Gastrins