title: Anterior cruciate ligament allograft transplantation. Long-term function, histology, revascularization, and operative technique.
abstract: In recent years much effort has been devoted to finding a satisfactory replacement for the injured ACL. None of the reconstruction techniques used in the past can be considered ideal because of their inability to duplicate the complex geometry, structure, and function of the ligament. Current advances in allograft transplantation and cryopreservation have led us to design and implement an experimental model for testing the feasibility of cryopreserved ACL allotransplantation. Groups of dogs were used to evaluate the effect of cryopreservation on ligament strength and to compare the relative performance of both autograft and allograft ACL transplants up to 18 months after implantation. The ligaments were examined mechanically, histologically, and microangiographically. The cryopreservation process and duration of storage had no effect on the biomechanical or structural properties of the ligament. The mechanical integrity of the allografts was similar to that of the autografts, with both achieving nearly 90% of control ligament strength by 36 weeks. Revascularization approached normal by 24 weeks in both autograft and allograft. No evidence of structural degradation or immunological reaction was seen. Based on these results, we believe that a cryopreserved ACL allograft can provide the ideal material for ACL reconstruction. We have outlined a surgical technique for harvesting and implanting this graft clinically.
mesh_terms: D000818:Animals; D001863:Bone Screws; D004285:Dogs; D007719:Knee Joint / Q000601:surgery*; D008023:Ligaments, Articular / Q000033:anatomy & histology / Q000098:blood supply / Q000502:physiology / Q000637:transplantation*; D008722:Methods; D013530:Surgical Wound Infection / Q000209:etiology