title: Electrochemical determination of hemoglobin, hematocrit, and hemolysis.
abstract: Novel electrochemical methods have been developed for determination of total hemoglobin, hematocrit, and detection of hemolysis in whole blood. Hemoglobin is measured through its peroxidase activity, a fluoride ion-selective electrode being used to monitor the rate of fluoride ion production from the oxidation of an organofluorine compound. Results agree well with those obtained with the cyanmethemoglobin method (r = 0.970). Hematocrit is determined from the ratio of the sodium ion concentrations measured with an ion-selective electrode before and after lysis of the erythrocytes. Results by this and the microhematocrit method correlated well (r = 0.987). Hemolysis in a whole-blood sample is detected by using an oxygen electrode to measure the oxygen released when hemoglobin in plasma is oxidized.
mesh_terms: D001555:Benzene Derivatives; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004912:Erythrocytes / Q000032:analysis; D005459:Fluorides / Q000032:analysis; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis*; D006461:Hemolysis; D006801:Humans; D006845:Hydrocarbons, Fluorinated; D008433:Mathematics; D010100:Oxygen / Q000032:analysis
chemical_list: D001555:Benzene Derivatives; D006454:Hemoglobins; D006845:Hydrocarbons, Fluorinated; C007164:cumene hydroperoxide; D005459:Fluorides; D010100:Oxygen