title: Biosynthesis of Rhizobium trifolii capsular polysaccharide: enzymatic transfer of pyruvate substitutions into lipid-bound saccharide intermediates.
abstract: The activity of capsular polysaccharide pyruvyltransferase catalyzing the pyruvylation of acidic heteropolysaccharide was measured in Rhizobium trifolii 843 and 0403 rif. This enzyme activity was determined with EDTA-treated cells, uridine diphosphate-sugar precursors, and phosphoenol [1-14C]pyruvate. Activity was measured by the incorporation of radioactivity into organic solvent-soluble glycoconjugates. Enzymatic pyruvylation of capsular polysaccharide occurred from phosphoenolpyruvate at the lipid-bound saccharide stage.
mesh_terms: D019880:Aldehyde-Ketone Transferases; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012231:Rhizobium / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism
chemical_list: D011135:Polysaccharides, Bacterial; D014539:Uridine Diphosphate Sugars; D010728:Phosphoenolpyruvate; D014166:Transferases; D019880:Aldehyde-Ketone Transferases; C050676:polysaccharide pyruvyltransferase