Synthesis of branched cyclomalto-oligosaccharides using Pseudomonas isoamylase.
Branched cyclomalto-oligosaccharides (cyclodextrins) were synthesised from cyclomalto-oligosaccharides and maltose or maltotriose through the reverse action of Pseudomonas isoamylase. The reaction rate was greater with maltotriose than with maltose, and with increasing size of the cyclomalto-oligosaccharide (cG6 less than cG7 less than cG8). Maltotriose is effective as both a side-chain donor and acceptor, and three isomers of 6-O-alpha-maltotriosylmaltotriose (branched G6) were formed through mutual condensation, but maltose was effective only as a side-chain donor. Each branched cyclomalto-oligosaccharide and G6 was purified by liquid chromatography, and their structures were determined by chemical, enzymic, and 13C-n.m.r. spectroscopic analyses.
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000138:chemical synthesis*; D003912:Dextrins / Q000138:chemical synthesis*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007517:Isoamylase / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011549:Pseudomonas / Q000201:enzymology*; D013213:Starch / Q000138:chemical synthesis*; D013379:Substrate Specificity
D003505:Cyclodextrins; D003912:Dextrins; D013213:Starch; D006026:Glycoside Hydrolases; D007517:Isoamylase