The structure of the heptose-3-deoxy-D-mannooctulosonic-acid region in a mutant form of Aeromonas salmonicida lipopolysaccharide.
Lipopolysaccharide was isolated from a phage-selected mutant of a wild strain of Aeromonas salmonicida by the aqueous phenol method. The lipopolysaccharide consisted of the R form, containing per mole, three moles of L-glycero-D-manno-heptopyranose, one mole of 3-deoxy-D-manno-2-octulosonic acid (dOclA) and lipid A. The dOclA was not fully assayable by the thiobarbituric acid methods usually used, but its degradation product was detected, after Smith degradation of the lipopolysaccharide, either as free 3-deoxy-2-heptulosonic acid (after hydrolysis) or substituted by a mannopyranosyl residue derived from heptose. Mass spectrometry indicated that the dOclA existed in the furanose form and was substituted by the heptose trisaccharide through position six. Methylation analysis, chemical degradation, chromium trioxide oxidation and nuclear magnetic resonance spectroscopy were used to identify the structure of the core oligosaccharide as: L alpha DHepp(1----2)L alpha DHepp(1----3)L alpha DHepp(1----6)dOclAf(2----.
D000107:Acetylation; D000333:Aeromonas / Q000032:analysis* / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000235:genetics / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009154:Mutation; D009844:Oligosaccharides / Q000302:isolation & purification; D010084:Oxidation-Reduction
D008070:Lipopolysaccharides; D009844:Oligosaccharides