Identification of chicken major histocompatibility complex (B) haplotypes by an immunoblot technique.
A means of identifying the major histocompatibility complex (MHC) phenotype of chickens has been developed using solubilised extracts of cell membranes. The proteins from the solubilised membranes are separated by gel electrophoresis, applied to nitrocellulose membranes and detected using specific alloantisera followed by biotin-conjugated secondary antisera and avidin-glucose oxidase complex. This procedure allows samples to be stored frozen for at least 2 years before analysis and in conjunction with Western blotting allows characterisation of the class of MHC-encoded protein identified by the antisera.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D007202:Indicators and Reagents; D008285:Major Histocompatibility Complex
D006649:Histocompatibility Antigens; D007202:Indicators and Reagents