Kinetics of energy consumption in human platelets with blocked ATP regeneration.
Abrupt arrest of ATP resynthesis in blood platelets induces a rapid decline in metabolic ATP-ADP. This decline is biexponential with a 7-fold difference in the rate-constants of the two components. Stimulation with thrombin increases both rate-constants, and raises the relative contribution of the rapid component from 60 to 90% of total. The initial decline can be approximated by a single exponential term, yielding the rate-constant for initial ATP hydrolysis. Since this initial decline reflects energy consumption of undisturbed platelets, this approach offers a sensitive means to determine energy consumption and ATP turnover within short time intervals.
D000244:Adenosine Diphosphate / Q000097:blood*; D000255:Adenosine Triphosphate / Q000097:blood*; D000968:Antimycin A / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D004734:Energy Metabolism; D005942:Gluconates / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007783:Lactones; D013917:Thrombin / Q000502:physiology
D005942:Gluconates; D007783:Lactones; D000244:Adenosine Diphosphate; D000968:Antimycin A; D000255:Adenosine Triphosphate; D013917:Thrombin; C010730:beta-glucono-1,5-lactone