title: [Changes in the structural-functional state of erythrocyte membranes treated with anion transport inhibitors].
abstract: Effect of 4,4-dyisotiocyanostilben-2,2-disulfonate (DIDS) and 1-ftor-2,4-dinitrobenzol (NDFB) on the rate of phosphate ion transport in erythrocytes, filtrability and thermal stability of erythrocytes and on the structural state of the erythrocyte membrane estimated by UV-fluorescence, PAAG--electrophoresis and measuring of the activity of membrane-bound acetylcholinesterase (AChE) has been studied. Unpenetrating anion transport inhibitor DIDS is shown to induce structural modifications of bands 3 of protein and AChE, while DNFB penetrating the membrane causes a significant reorganization of many membrane proteins (including spectrin) resulting in changes of transport and mechanical properties of erythrocytes.
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000031:analogs & derivatives*; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000110:Acetylcholinesterase / Q000097:blood; D001692:Biological Transport / Q000187:drug effects; D002463:Cell Membrane Permeability; D004139:Dinitrofluorobenzene / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000097:blood; D009578:Nitrobenzenes / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D008565:Membrane Proteins; D009578:Nitrobenzenes; D010710:Phosphates; D013267:Stilbenes; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C018564:dihydro-DIDS; D004139:Dinitrofluorobenzene; D000110:Acetylcholinesterase; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
vernacular_title: Izmenenie strukturno-funktsional'nogo sostoianiia membran éritrotsitov pod vozdeĭstviem ingibitorov transporta anionov.