Electron microscopy microsampling of isolated nuclei sorted by flow cytometry.
This paper describes an efficient method to concentrate for electron microscopic examination minute quantities of subcellular particles obtained by cytofluorimetric sorting. The advantages of this micromethod, based on diafiltration on Millipore filters under constant positive nitrogen pressure, are discussed.
D000818:Animals; D002467:Cell Nucleus; D002469:Cell Separation; D005434:Flow Cytometry; D008099:Liver / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D051381:Rats; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*