Low temperature high-performance liquid chromatography of cis-trans proline dipeptides.
Isomeric dipeptides which contain proline at the C-terminus and undergo on-column cis-trans isomerization were separated by reversed-phase chromatography at subambient column temperature. Chromatography at low temperatures offers a convenient method for measuring the relative concentrations of the isomers and, thus, the calculation of the equilibrium constants for such cis-trans isomerization at various conditions of solvent, pH, and temperature. Pure fractions of the cis and trans isomers were collected and used in kinetic studies of the isomerization. High-performance liquid chromatography at temperatures near the freezing point of the eluent is a potentially useful tool for the study of a wide range of biochemical molecules.
D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D007700:Kinetics; D011392:Proline / Q000032:analysis*; D013696:Temperature
D004151:Dipeptides; D011392:Proline