Determination of polypeptide chain molecular weights of human and bovine band 3 protein from erythrocyte membranes by low-angle laser light scattering combined with high-performance gel chromatography in the presence of sodium dodecyl sulfate.
Polypeptide chain molecular weights of human and bovine band 3 proteins which are glycoproteins of the erythrocyte membrane were determined as 101,000 +/- 2000 for the former and 107,000 +/- 2000 for the latter by using the low-angle laser light scattering technique combined with a high-performance gel chromatography column, an ultraviolet spectrophotometer and a differential refractometer in the presence of sodium dodecyl sulfate. The advantage of this method is that, unlike the sedimentation equilibrium technique, neither information on the binding to proteins of all ligands present nor the partial specific volume is required to evaluate the polypeptide chain molecular weight of proteins in a multicomponent system.
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000302:isolation & purification; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004910:Erythrocyte Membrane / Q000032:analysis; D006801:Humans; D007834:Lasers; D046911:Macromolecular Substances; D008970:Molecular Weight; D012542:Scattering, Radiation; D013045:Species Specificity
D001457:Anion Exchange Protein 1, Erythrocyte; D046911:Macromolecular Substances