title: Reversible disruption of lordosis via midbrain infusions of procaine and tetrodotoxin.
abstract: Behavioral effects of bilateral intracranial infusions of tetrodotoxin (1, 3.3 or 10 ng/rat), 50% procaine (2 microliters/rat) or phosphate-buffered saline (PBS-2 microliters/rat) into the dorsal midbrain of conscious, lightly-restrained female rats were evaluated. High levels of lordotic responsiveness were induced in ovariectomized animals treated with estradiol (E2) capsules or subcutaneous injections of estradiol benzoate (EB) followed by progesterone (P). The effect of each of the 3 infusates on lordosis was determined using manual stimulation and lordosis quotient determinations. In addition, the vocalization by an animal during lordosis measurements, paw withdrawal to pinch, righting reflex latency and recognition of a platform edge were also monitored. Within 2 minutes following procaine or tetrodotoxin (TTX) infusions in E2 implanted rats, lordotic responsiveness declined sharply. Whereas procaine-treated animals returned to control levels of responsiveness within 20 minutes, TTX infusions induced a more prolonged depression of lordosis lasting up to 8 hours. Infusions of PBS had no effect on any of the behaviors. In a separate group of animals treated with either E2 or EB + P and infused with 10 ng TTX the time course of the decline in lordotic responsiveness was identical for both steroid treatments. Paw withdrawal was unaffected by TTX while all other measured behaviors were disrupted along the same time course as lordosis. Collectively the above results implicate the requirement of sodium-dependent neuronal activity within dorsal midbrain for the maintenance of the lordosis reflex, along with other behavioral responses influenced by this brain region.
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004343:Drug Implants; D004958:Estradiol / Q000502:physiology; D005260:Female; D007263:Infusions, Parenteral; D008636:Mesencephalon / Q000187:drug effects*; D011343:Procaine / Q000494:pharmacology*; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology*
chemical_list: D004343:Drug Implants; D013779:Tetrodotoxin; D011374:Progesterone; D004958:Estradiol; D011343:Procaine