title: Non-specific interactions of histones with serum components and contractile proteins.
abstract: Strong, apparently non-specific interactions are often observed between antisera and proteins in the assay of biological systems by methods employing antibodies. We have documented the interaction between immunoglobulin-enzyme complexes and nuclear histone proteins. This binding has been demonstrated for all the histones derived from bovine thymus. The interaction between histones and the immunoglobulin-enzyme complexes has been shown to be inhibited by both whole serum and individual serum components as well as a number of the major contractile proteins.
mesh_terms: D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006657:Histones / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D001798:Blood Proteins; D006657:Histones; D007074:Immunoglobulin G; D009124:Muscle Proteins