Efficacy of performing red-cell antibody elutions in patients with a positive direct antiglobulin test.
Red blood cell antibody elutions are often routinely performed whenever a positive direct antiglobulin test (DAT) is encountered. To evaluate the efficacy of performing routine red-cell elutions we reviewed our antibody elution data. Of 122 eluates, 83 were negative, 35 were warm panagglutinins, 2 were felt to be transfusion-induced alloantibodies, 1 was passively acquired anti-A, and 1 was inconclusive. One of the eluted alloantibodies was not demonstrable in the serum. Thus, only 1 (0.8%) of the eluates provided important information not readily available through serum testing alone. We conclude that extensive serologic evaluation of a positive DAT should be reserved for those patients who have been recently transfused or are suspected of having immune hemolysis.
D000373:Agglutinins / Q000032:analysis; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis; D001803:Blood Transfusion; D003298:Coombs Test; D003450:Cryoglobulins; D004912:Erythrocytes / Q000276:immunology*; D006388:Hemagglutinins / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007518:Isoantibodies / Q000032:analysis
D000373:Agglutinins; D001323:Autoantibodies; D003450:Cryoglobulins; D006388:Hemagglutinins; D007074:Immunoglobulin G; D007518:Isoantibodies; C029443:cold agglutinins