Cell-density-dependent uptake of chylomicron remnants in rat hepatocyte monolayers. Effects of compactin and mevalonic acid.
In rat hepatocytes cultured in lipoprotein-deficient serum, the uptake and degradation of chylomicron remnant cholesteryl ester per mg cell protein varies inversely with cell density. Compactin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme-A reductase, stimulates the uptake at all cell densities. Mevalonic acid, on the other hand, can suppress a significant part of the remnant uptake. Chylomicron remnant uptake in hepatocyte cultures can thus be influenced by factors known to regulate the apolipoprotein-BE receptor activity.
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D002914:Chylomicrons / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008148:Lovastatin / Q000031:analogs & derivatives*; D008297:Male; D008798:Mevalonic Acid / Q000494:pharmacology*; D009281:Naphthalenes / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D002914:Chylomicrons; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008041:Linoleic Acids; D009281:Naphthalenes; C012258:mevastatin; D002784:Cholesterol; D019787:Linoleic Acid; D008148:Lovastatin; D008798:Mevalonic Acid