Caseins are cross-linked through their ester phosphate groups by colloidal calcium phosphate.
Artificial casein micelles were prepared by adding 30 mM calcium, 22 mM phosphate and 10 mM citrate to sodium caseinate solutions, and the content of the casein aggregates cross-linked by colloidal calcium phosphate was determined by high-performance gel chromatography on a TSK-GEL G4000SW column in the presence of 6 M urea. The content of the casein aggregates cross-linked by colloidal calcium phosphate in artificial whole casein micelles was 48% of total casein, and their relative casein composition determined by high-performance ion-exchange chromatography was 53.1% for alpha s1-casein, 15.8% for alpha s2-casein, 31.1% for beta-casein and 0% for kappa-casein. The order of cross-linking by colloidal calcium phosphate agreed with that of the ester phosphate content of casein constituents. The content of the casein aggregates cross-linked by colloidal calcium phosphate was higher in alpha s1-kappa-casein micelles than in beta-kappa-casein micelles. kappa- and gamma-caseins and dephosphorylated alpha s1-casein were not cross-linked by colloidal calcium phosphate. Although kappa-casein was not cross-linked, chemically phosphorylated kappa-casein, of which the average phosphate content was 8.5 per molecule, was cross-linked. It is concluded that caseins are cross-linked through their ester phosphate groups by colloidal calcium phosphate.
D000818:Animals; D002118:Calcium; D002364:Caseins; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002951:Citrates; D003102:Colloids; D003432:Cross-Linking Reagents; D004952:Esters; D008823:Micelles; D010710:Phosphates
D002364:Caseins; D002951:Citrates; D003102:Colloids; D003432:Cross-Linking Reagents; D004952:Esters; D008823:Micelles; D010710:Phosphates; D002118:Calcium