Novel separation method for serum immunoglobulins. Application to thyroid related antibodies.
A dye-based affinity chromatographic system using Remazol yellow GGL-Sepharose is described for the fractionation of serum immunoglobulins. Immunoglobulins are sequentially eluted from the gel columns using gradients of pH and salt with greater than 88% recovery. Specific immunoglobulin activities were identified as discrete peaks and antibodies raised against the same antigen were separated. Biological properties of antibodies were retained following chromatography. The method is applicable to both human and animal immunoglobulins.
D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000145:classification; D005591:Chemical Fractionation / Q000379:methods; D002845:Chromatography / Q000379:methods*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D011989:Receptors, Thyrotropin / Q000276:immunology*
D000906:Antibodies; D007136:Immunoglobulins; D011989:Receptors, Thyrotropin