[Interaction of hormone-receptor complexes from normal and tumorous tissues with DNA and their effect on the RNA polymerase activity of isolated nuclei].
A study was made of the interaction of the thyroxine hormone-receptor complexes (HRC) from hepatocytes and sarcoma M1 cells with DNA and the involvement of these complexes in the regulation of the RNA-polymerase activity of isolated hepatic cell nuclei. Unlike sarcoma HRC, rat liver HRC showed a weak interaction with DNA. Against a background of considerable stimulation of the incorporation of precursors in RNA under the influence of rat liver HRC (2.64 times), sarcoma HRC increased RNA synthesis in the system by 31.7% only to determine the RNA-polymerase activity of the nuclei. Proceeding from these results the authors have concluded that a thyroxine-binding receptor protein in malignant tissues is devoid of biological activity in the regulation of gene expression by the thyroid hormones, at least in the transcription phase.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000378:metabolism*; D013974:Thyroxine / Q000378:metabolism*
D004273:DNA, Neoplasm; D011988:Receptors, Thyroid Hormone; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D013974:Thyroxine
Vzaimodeĭstvie gormon-retseptornykh kompleksov iz normal'noĭ i opukholevoĭ tkani s DNK i ikh vliianie na RNK-polimeraznuiu aktivnost' izolirovannykh iader.