title: Secretion of a 97 kilodalton glycoprotein by primary cultures of human ovarian epithelial tumor cells.
abstract: The epithelial cells from both control and neoplastic ovaries were grown as primary cultures. The outgrowth of epithelial cells occurred within 3-5 days and the cells formed essentially a monolayer culture covering more than 80% of the surface of the flask within 5 weeks. The incorporation of [3H]fucose into the glycoproteins secreted into the medium was measured in the cells grown for 4 weeks. The secretion of glycoproteins in the tumor cells was twice that of the control. Analysis of the glycoproteins in the medium showed that the ovarian epithelial tumor cells secreted predominantly a 97 kilodalton glycoprotein. The growth of fibroblasts could be inhibited in these cultures by using Falcon Primaria culture flasks and the growth medium containing D-valine.
mesh_terms: D002277:Carcinoma / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D010051:Ovarian Neoplasms / Q000378:metabolism*; D014633:Valine / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; D005643:Fucose; D014633:Valine