Kinetic studies on the lactate dehydrogenase isozymes of the brown trout, Salmo trutta L.
Informative crosses have verified the genetic basis of a polymorphism at the Ldh-1 locus in brown trout and enzyme activity measurements indicate that the previously described polymorphism at this locus is best explained by a null allele. The LDH-1, LDH-2, LDH-3 and LDH-4 homotetrameric isozymes were purified and subjected to enzyme kinetic analysis. While LDH-1 and LDH-2 displayed catalytic equivalence, important kinetic differences were found between the LDH-3 and LDH-4 isozymes.
D000818:Animals; D003433:Crosses, Genetic; D005838:Genotype; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D046911:Macromolecular Substances; D009132:Muscles / Q000201:enzymology*; D009928:Organ Specificity; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D007527:Isoenzymes; D046911:Macromolecular Substances; D007770:L-Lactate Dehydrogenase