Dexamethasone reduces the high-affinity binding of hemicholinium-3 to rat phrenic nerve endings in vitro: an autoradiographic study.
Receptor autoradiography has revealed high-affinity binding sites for hemicholinium-3 (HC-3) in the phrenic nerve endings of rat diaphragm. It has been demonstrated that 200 nM dexamethasone (Dex) in vitro reduces the binding of HC-3 to these high-affinity sites. It seems likely that the decrease in inhibitory effects of HC-3 on choline uptake and acetylcholine synthesis in rat diaphragm caused by Dex, should be the result of an interference of Dex with the binding of HC-3 to its high affinity sites.
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D003907:Dexamethasone / Q000494:pharmacology*; D003964:Diaphragm / Q000294:innervation; D005260:Female; D006426:Hemicholinium 3 / Q000378:metabolism*; D066298:In Vitro Techniques; D009045:Motor Endplate / Q000187:drug effects / Q000378:metabolism; D009411:Nerve Endings / Q000187:drug effects / Q000378:metabolism*; D010791:Phrenic Nerve / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D006426:Hemicholinium 3; D003907:Dexamethasone