Purification and characterization of a major allergen from the house dust mite Dermatophagoides farinae.
A major allergen from an extract of the house dust mite, Dermatophagoides farinae, was shown to be extremely heterogenic with respect to charge. A slightly basic component of this allergen with a pI of 8, was purified by isoelectric focusing in two steps. The purified component, denoted antigen 19/20 IIa, seemed to be representative for the allergenic activity of the major allergen. The amino acid analysis suggested that antigen 19/20 IIa had a molecular weight of 9400 and contained one residue of galactosamine. SDS polyacrylamide gel electrophoresis did indicate a somewhat higher molecular weight of 14,500. Antibodies against the purified component cross-reacted with a crude extract of the mite Dermatophagoides pteronyssinus.
D000485:Allergens / Q000032:analysis / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004391:Dust; D005243:Feces / Q000276:immunology; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007526:Isoelectric Point; D008925:Mites / Q000276:immunology*; D008970:Molecular Weight; D011852:Radioallergosorbent Test; D012882:Skin Tests
D000485:Allergens; D000596:Amino Acids; D004391:Dust