Application of polyacrylamide gel electrophoresis of genome fragments to control of reovirus products.
The reoviridae are notable for their genome consisting of 10 to 12 pieces of double-stranded RNA. The migration pattern of these pieces produced by polyacrylamide gel electrophoresis has been used by other workers as a means of identifying these viruses for epidemiology or classification. This paper reports attempts by the authors to apply this technique to various aspects of control of reovirus products, with a view to supplementing or replacing techniques involving animals. The findings are discussed in relation to those of other workers.
D004591:Electrophoresis, Polyacrylamide Gel; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000032:analysis* / Q000145:classification / Q000235:genetics
D012330:RNA, Double-Stranded; D012367:RNA, Viral