Pattern of labeling of rabbit cerebral cortex soluble proteins 24 hours after a single electroconvulsive shock.
The labeling of proteins in the particulate subcellular fractions and in the cell sap was studied in the cerebral cortex of rabbits 24 hours after a single electroconvulsive shock (ECS). To this end the animals were injected with [3H]valine subarachnoidally and sacrificed 30 minutes later. The incorporation of the labeled amino acid into proteins was slightly increased in all subcellular fractions but the increase was significant only for soluble proteins. Referring to the type of soluble polypeptides found in the cortex at this post ECS phase, no modification was found in the SDS electrophoresis pattern of steady state proteins. The fractionation pattern of labeled soluble proteins showed in one third (3 out of 9) of the experiments a remarkable stimulation of the synthesis of two polypeptides (25,000 and 54,000 Dalton MW), which were not labeled in the controls.
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D004586:Electrophoresis; D004597:Electroshock; D005260:Female; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011817:Rabbits; D012640:Seizures / Q000378:metabolism*; D012995:Solubility; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D014633:Valine / Q000378:metabolism
D009419:Nerve Tissue Proteins; D014633:Valine