Proliferative activity in incubated slices of immature rat cerebellum.
Slices of 8-day-old rat cerebella were prepared with a tissue chopper, incubated in an oxygenated Krebs-Henseleit solution followed by processing for histology. One micrometer thick sections of resin-embedded slices were stained with Toluidine blue. The external granular layer was shown to maintain its normal proliferative activity up to 3 h of incubation as suggested by the presence of all mitotic phases within this period and by a roughly 50% increase in mitotic index upon a 90-min exposure of incubated slices to 20 microM vinblastine. On this basis the incubated slice of the immature rat cerebellum may serve as a simple tool for the rapid histological testing of short-term mitogenic or cytostatic effects and the vulnerability of a brain germinal layer.
D000818:Animals; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008940:Mitotic Index; D051381:Rats; D014747:Vinblastine / Q000494:pharmacology
D014747:Vinblastine