title: 120 and 80 kd detergent-insoluble glycoproteins as markers of differentiation and adhesion in B16 melanoma.
abstract: Surface glycoproteins correlating with cell adhesion and differentiation have now been studied in pigmented and amelanotic B16 melanoma cell monolayers and aggregates, labelled with 3H-glucosamine. Solubilization with the non-ionic detergent octyl glucoside revealed mostly adhesion-related changes, which involved a decrease in 140 and 110 kd glycoproteins in both pigmented and amelanotic cell aggregates. Nevertheless, differentiation-regulated changes were preferentially evident in the remaining detergent-insoluble glycoproteins. Pigmented cell monolayers showed a preferential increase in glycoprotein species of about 120 kd and 80 kd, which were not detected in amelanotic monolayers or in pigmented or amelanotic cell aggregates. Our findings suggest the relevance of these cytoskeleton associated glycoproteins as markers of differentiation and adhesion in melanoma cells.
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000302:isolation & purification* / Q000502:physiology; D003902:Detergents; D006023:Glycoproteins / Q000302:isolation & purification* / Q000502:physiology; D008545:Melanoma; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000502:physiology; D010858:Pigmentation; D012995:Solubility
chemical_list: D003598:Cytoskeletal Proteins; D003902:Detergents; D006023:Glycoproteins; D009363:Neoplasm Proteins