Analysis of the expression of H-2K, D and L locus mRNAs by use of an oligonucleotide primer.
By employing an oligonucleotide-dependent cDNA synthesis/HaeIII digestion procedure followed by gel electrophoretic separation of HaeIII digested cDNAs, we have developed a method for analyzing the expression of H-2 mRNAs encoding the K, D and L locus histocompatibility antigens. This method provides unambiguous data as the individual K, D or L specific cDNA bands can be isolated from the gel and partially sequenced. The method is readily applicable to analyzing the relative expression of K, D and L locus mRNAs in normal mouse tissues and their tumors.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D001483:Base Sequence; D004247:DNA; D006183:H-2 Antigens / Q000235:genetics*; D007939:Leukemia L1210 / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000032:analysis*
D000951:Antigens, Neoplasm; D006183:H-2 Antigens; D009841:Oligonucleotides; D012333:RNA, Messenger; D004247:DNA