Metabolic cooperation in vitro: differential ability of ouabain to uncouple normal, preneoplastic, and neoplastic mouse mammary cells.
We utilized two assays for metabolic cooperation in vitro, one in which cells were grown in monolayer and one in which the cells formed three-dimensional structures in a collagen gel matrix. Both assays required one of the test cell pair to be resistant both to ouabain and to thioguanine (deficient in hypoxanthine-guanine phosphoribosyltransferase). Normal mammary gland cells, cells from preneoplastic mouse hyperplastic alveolar nodules, and mouse mammary tumor cells were metabolically linked in vitro to the drug-resistant tumor cells. Ouabain abrogated communication between tumor cells and normal mammary gland cells and between tumor cells and preneoplastic cells but had no effect on communication between tumor cells.
D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D004351:Drug Resistance; D005260:Female; D066298:In Vitro Techniques; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010042:Ouabain / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000378:metabolism; D013866:Thioguanine / Q000494:pharmacology
D010042:Ouabain; D013866:Thioguanine