title: Immunoblot analysis of circulating antibodies against muscle proteins in amyotrophic lateral sclerosis and other neurologic diseases.
abstract: Serum from patients with amyotrophic lateral sclerosis (ALS) was tested by immunoblotting for reactivity against three muscle-derived preparations: denervated chick leg muscle extracts, media conditioned by denervated rat hemidiaphragms, and a human muscle extract. For each preparation, multiple bands were present using serum from all patients and controls, and no band was unique to ALS. Against rat muscle-conditioned medium, bands in the 56,000 (56K) molecular weight range were present to an equal degree in ALS and in controls; in addition, different bands near 56K were recognized by serum from different ALS patients. These results fail to confirm a recent report suggesting that anti-56K reactivity is characteristic of ALS.
mesh_terms: D000328:Adult; D000690:Amyotrophic Lateral Sclerosis / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D009103:Multiple Sclerosis / Q000276:immunology; D009124:Muscle Proteins / Q000276:immunology*; D009157:Myasthenia Gravis / Q000276:immunology; D009422:Nervous System Diseases / Q000276:immunology*; D051381:Rats
chemical_list: D000906:Antibodies; D009124:Muscle Proteins