Laminar elution of tubular organs for bioluminescence analysis of the interior cell layer.
Cells lining the interior of tubular organs are of considerable interest from physiological and pathological aspects but are very difficult to prepare for biochemical analyses. The contents of such cells can be extracted by infusion of a suitable detergent serving as a membrane destroyer. The tiny ureter of the rat has been used as experimental model. Time governed elution with saponin, using a Hamilton programmable microlab for the infusion results in an effluent pattern which can be determined by sensitive bioluminescence assays. The time course of the outflux of nucleotides and enzymes showed two maxima in agreement with the presence of two epithelial layers in the ureter.
D000227:Adenine Nucleotides / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D004798:Enzymes / Q000032:analysis; D008163:Luminescent Measurements; D009243:NAD / Q000032:analysis; D051381:Rats; D012503:Saponins; D012995:Solubility; D013997:Time Factors; D014513:Ureter / Q000032:analysis* / Q000648:ultrastructure
D000227:Adenine Nucleotides; D004798:Enzymes; D012503:Saponins; D009243:NAD; D000255:Adenosine Triphosphate