Structure determination of the O-specific polysaccharides from Citrobacter O4- and O27-lipopolysaccharides by methylation analysis and one- and two-dimensional 1H-NMR spectroscopy.
Using sugar and methylation analyses, and one- and two-dimensional 1H-NMR spectroscopy at 500 MHz it was established that poly-beta-1,2-4-deoxy-D-arabinohexopyranose occurs as O-specific chains of lipopolysaccharides in Citrobacter serotypes O4, O27 and O36. Strong serological cross-reactivity between these serotypes is in full agreement with the chemical identity of their O-specific polysaccharides.
D055598:Chemical Phenomena; D002621:Chemistry; D002954:Citrobacter; D006859:Hydrogen; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011135:Polysaccharides, Bacterial; D013057:Spectrum Analysis / Q000379:methods
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D006859:Hydrogen