title: Proliferation of the surface-connected intracytoplasmic membranous network in skeletal muscle disease.
abstract: A surface-connected intracytoplasmic membranous (SCIM) network proliferates in skeletal muscle diseases and in myotubes grown in vitro. The authors observed frequent occurrence of "coated" microdomains in the form of budding vesicles in the proliferated components of this network and suspected a potential role the proliferated membranes might have in the endocytosis of molecules into myotubes undergoing repair or regeneration. Five-day-old myotubes in culture were incubated at 37 C and between 2 and 4 C with two tracers, Lucifer yellow and ferritin, both known to enter other types of cells via a fluid-phase endocytotic pathway. The differential penetration of Lucifer yellow at 37 C and below 2-4 C was examined by fluorescence microscopy and by electron microscopy. Lucifer yellow was rendered electron-opaque by photoreacting it with an intense light in the presence of DAB. Ferritin penetration at 37 C and between 2 and 4 C was compared and quantitated ultrastructurally. The authors found that endocytosis of the tracers into myotubes and eventually into lysosomes took place after the tracers had diffused into the lumen of the proliferated SCIM network. These processes were inhibited below 4 C. This finding, coupled with the presence of "coated" microdomains in the proliferated membranes, led us to suspect that the SCIM network may have a role in membrane turnover of metabolically active diseased muscle cells undergoing regeneration.
mesh_terms: D000818:Animals; D002455:Cell Division; D002462:Cell Membrane / Q000648:ultrastructure; D002642:Chick Embryo; D004705:Endocytosis; D005293:Ferritins; D006651:Histocytochemistry; D007546:Isoquinolines; D008854:Microscopy, Electron; D009132:Muscles / Q000473:pathology*; D009135:Muscular Diseases / Q000473:pathology*
chemical_list: D007546:Isoquinolines; D005293:Ferritins; C017475:lucifer yellow