Enzymatic characterization of Babesia bovis.
Agarose gel electrophoresis was used to identify metabolic enzymes in Babesia bovis and B. bigemina. Glutamate dehydrogenase, lactate dehydrogenase, glucose phosphate isomerase, and hexokinase were identified in B. bovis- and B. bigemina-infected erythrocytes and B. bovis merozoite preparations. A specific electrophoretic mobility was observed for each enzyme. Malate dehydrogenase, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and adenylate kinase were only detected in normal erythrocyte preparations. Inter-species, but not intra-species, variation was noted when comparing electrophoretograms of both species. Kinin-activating activity was not detected in B. bovis-infected erythrocyte or merozoite preparations at pH 4.2 or 7.6.
D000263:Adenylate Kinase / Q000032:analysis / Q000097:blood; D000818:Animals; D001403:Babesia / Q000201:enzymology*; D002417:Cattle; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000201:enzymology / Q000469:parasitology; D004950:Esterases / Q000032:analysis / Q000097:blood; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000097:blood; D005969:Glutamate Dehydrogenase / Q000032:analysis*; D006593:Hexokinase / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis / Q000097:blood; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis / Q000097:blood
D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D006593:Hexokinase; D000263:Adenylate Kinase; D004950:Esterases; D005956:Glucose-6-Phosphate Isomerase