Effect of zwitterionic buffers on measurement of small masses of protein with bicinchoninic acid.
Linear standard curves were obtained for 1 to 10 micrograms of bovine serum albumin measured in the presence of 50 mM Mes, Mops, Pipes, Caps, Bes, Hepes, Heppso, and Tes zwitterionic buffers using bicinchoninic acid for detection. The zwitterionic buffers Ada, Tricine, and Bicine that strongly bind Cu2+. Tris, and Bistris, each at 50 mM, inhibited color development to prevent quantitative measurement of protein mass.
D002021:Buffers; D011506:Proteins / Q000032:analysis*; D011804:Quinolines; D012015:Reference Standards; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000592:standards
D002021:Buffers; D011506:Proteins; D011804:Quinolines; D012710:Serum Albumin, Bovine; C047117:bicinchoninic acid