title: Is thermally denatured protein unfolded? The example of alpha-lactalbumin.
abstract: The degree of unfolding of various forms of human and bovine alpha-lactalbumin was characterized by the chromatographically determined distribution coefficient. Molecular size increases as follows: native protein approximately equal to apo form less than acid form much less than thermally unfolded form approximately equal to guanidine hydrochloride-unfolded protein.
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007768:Lactalbumin; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation
chemical_list: D006146:Guanidines; D007768:Lactalbumin; D019791:Guanidine