Crossed immunoelectrophoretic analysis of ARDS lavage proteins.
Serum proteins in normal and ARDS bronchoalveolar lavages were analyzed using crossed immunoelectrophoresis. Normal lavages demonstrated relatively few proteins (albumin, alpha 1-antitrypsin, transferrin, and haptoglobin) in low concentrations. In contrast, substantial amounts of all identifiable serum proteins were detected in ARDS lavages. IgA was apparently locally produced. Two of the largest proteins, beta-lipoprotein (mol wt greater than 2 million) and IgM (mol wt approximately 900,000) were found to be complexed as evidenced by their coprecipitation in a single spike in ARDS lavage. Electrophoretic modifications of ARDS albumin and alpha 1-antitrypsin precipitation peaks and partial identity spurring of the alpha 1-lipoprotein peak with other precipitation loops indicated possible complex formation between these proteins and other possibly pathogenic lung fluid constituents. Similarly, modifications of orosomucoid and Gc-globulin peaks indicated possible molecular alterations resulting from interactions with other components. The relatively few protein modifications exhibited in ARDS lavages together with alpha 1-antitrypsin-protease complex formation confirm the relative absence of substantial proteolytic activity in ARDS edema fluids obtained within 12 hr of the onset of the syndrome demonstrated in previous studies.
D000418:Albumins / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D005110:Extracellular Space / Q000032:analysis; D005122:Exudates and Transudates / Q000032:analysis; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007136:Immunoglobulins / Q000032:analysis; D008077:Lipoproteins, LDL / Q000032:analysis; D008168:Lung / Q000032:analysis*; D011480:Protease Inhibitors / Q000032:analysis; D012128:Respiratory Distress Syndrome / Q000378:metabolism*; D014809:Vitamin D-Binding Protein / Q000032:analysis
D000418:Albumins; D001798:Blood Proteins; D007136:Immunoglobulins; D008077:Lipoproteins, LDL; D011480:Protease Inhibitors; D014809:Vitamin D-Binding Protein