Characterization of Ca2+-activated, phospholipid-dependent protein kinase C/protein kinase M in guinea pig peritoneal exudate macrophages.
Protein kinase C (C-kinase) is shown to be present in the cytosolic and particulate fractions of mineral oil induced peritoneal macrophages of guinea pigs. By omission or use of a high concentration of leupeptin, three forms of the enzyme were obtained: stimulant/Ca2+/phospholipid-dependent C-kinase, eluted from DEAE 52 cellulose at 0.08-0.16 M NaCl; stimulant/Ca2+/phospholipid-independent protein kinase M (M-kinase), and Ca2+-inhibited & stimulant/phospholipid-dependent form of protein kinase, both eluted from DEAE 52 cellulose at 0.18-0.22 M NaCl. Phorbol ester or 1,2-diacylglycerol were used as stimulants. It is suggested that Ca2+-inhibited & stimulant/phospholipid-dependent protein kinase represents the in vivo form of the M-kinase in intact cells.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002451:Cell Compartmentation; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation; D006168:Guinea Pigs; D007700:Kinetics; D007976:Leupeptins / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology*; D010446:Peptide Fragments / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010703:Phorbol Esters / Q000494:pharmacology; D010718:Phosphatidylserines / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*
D004075:Diglycerides; D007976:Leupeptins; D010446:Peptide Fragments; D010703:Phorbol Esters; D010718:Phosphatidylserines; D010743:Phospholipids; D011493:Protein Kinase C; C032854:leupeptin; D002118:Calcium