Crystallization and preliminary X-ray study of subunit III of the bovine pancreatic procarboxypeptidase A-S6 ternary complex.
Subunit III of the bovine pancreatic procarboxypeptidase A-S6 ternary complex was dissociated from the complex, purified and crystallized using the hanging- or sitting-drop method of vapour diffusion, with ammonium sulphate as the precipitant. The assays were carried out at pH 4.2 (20 mM-acetate buffer). An X-ray examination of the crystals shows that they are monoclinic, with a space group P21 and cell dimensions a = 47.9 A, b = 61.3 A, c = 39.0 A and beta = 95.0 degrees. The asymmetric unit contains one molecule of 25,800 Mr. The crystals are suitable for structure determination to at least 2.8 A resolution.
D000818:Animals; D002268:Carboxypeptidases / Q000302:isolation & purification; D043422:Carboxypeptidases A; D002417:Cattle; D003461:Crystallography; D004792:Enzyme Precursors / Q000302:isolation & purification; D046911:Macromolecular Substances; D010179:Pancreas / Q000201:enzymology*; D014961:X-Ray Diffraction
D004792:Enzyme Precursors; D046911:Macromolecular Substances; D002268:Carboxypeptidases; D043422:Carboxypeptidases A