[Tritium labeling of RNA and protein of bacteriophage MS2].
Thermal activation of tritium gas is used for labeling of the nucleoprotein, phage MS 2. The obtained preparation of tritiated phage has a specific radioactivity of 20-50 Ci/mmole, is considerably infectious and appears suitable for a wide range of studies. The radioactivity is distributed between intraphage RNA and phage outer protein (approximately 1:3 ratio). Consequently, phage capsid is porous and sufficiently permeable for activated tritium atoms.
D003090:Coliphages / Q000032:analysis*; D007553:Isotope Labeling; D012367:RNA, Viral / Q000032:analysis*; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis*
D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium
Vvedenie tritievoĭ metki v RNK i melok bakteriofaga MS2.