[Effect of a cerebral neurite-stimulating protein on the morphogenesis of an organotypical culture of spinal ganglia].
Cationic protein with molecular mass of 15,000 kDa has been extracted from the hemispheres of the rat and bovine brain. Addition of the protein into nutritive medium of the organo-typical culture of the chick embryo spinal ganglia results in a considerable (by 2-2.5 times) increase of the growth zone in the explants. Neurite-stimulating effect of the protein is demonstrate dat concentration of 10 ng/ml. An intensive longitudinal growth of neurites is observed, increase of their amount per area unit, an essential intensification of ramification, formation of powerful anastomoses, numerous dense plexuses and arcades. Fasciculi of neurites covered with glia are formed. The cerebral neurite-stimulating protein is, evidently, one of neuron-growing factors, regulating development of the nervous system in the organism.
D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D005727:Ganglia, Spinal / Q000254:growth & development*; D009024:Morphogenesis / Q000187:drug effects; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009928:Organ Specificity; D013268:Stimulation, Chemical
D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins
Vliianie mozgovogo neĭrit-stimuliruiushchego belka na morfogenez organotipicheskoĭ kul'tury spinnomozgovykh gangliev.