Comparison of TNF-alpha and TNF-beta cytolytic biological activities in a serum-free bioassay.
The effect of temperature and serum-free conditions on the cytolytic biological activities of TNF-alpha and TNF-beta were evaluated using murine connective tissue L-M as the target cell line. Serum-free assay medium and a 39 degrees C incubation temperature substantially enhanced the activity of both cytokines. L-M cells were 3 to 4 times more sensitive to cytolysis by TNF-beta than TNF-alpha under serum-free assay conditions.
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D003238:Connective Tissue / Q000187:drug effects; D003470:Culture Media; D003603:Cytotoxins; D006023:Glycoproteins / Q000494:pharmacology*; D051379:Mice; D013696:Temperature; D014409:Tumor Necrosis Factor-alpha
D003470:Culture Media; D003603:Cytotoxins; D006023:Glycoproteins; D014409:Tumor Necrosis Factor-alpha