Studies on the optimization of parameters of preparative liquid chromatographic columns for production of cardiac glycosides.
Investigations aimed at maximization of the output of the production of cardiac glycosides from their mixtures using liquid chromatography are reported. The dependences of the yield of the process on column length, particle size and surface area of the packing and linear velocity of the mobile phase were examined. It has been established that a simultaneous decrease in the particle size and an increase in the column length and mobile phase flow-rate results in an improvement in the yield. However, such a procedure is limited by technological considerations. Optimum production conditions have been characterized.
D002301:Cardiac Glycosides / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D010316:Particle Size
D002301:Cardiac Glycosides