Production of primate monoclonal antibodies.
Monoclonal antibodies of primate origin were produced by direct fusion of peripheral blood lymphocytes with two different fusion cell lines: SP2/0, a mouse plasmacytoma line and SBC-H2O, a human-mouse heteromyeloma. The fusion of primate lymphocytes with SP2/0 cells was not successful and only transient production of antibodies was found. The fusion of primate lymphocytes with SBC-H2O cells was more successful and resulted in the establishment of stable monoclonal antibody-producing lines especially when chimpanzee cells were used.
D000818:Animals; D000911:Antibodies, Monoclonal; D002459:Cell Fusion; D002460:Cell Line; D006801:Humans; D008251:Macaca / Q000276:immunology; D051379:Mice; D002679:Pan troglodytes / Q000276:immunology
D000911:Antibodies, Monoclonal