Hybridoma cultivation in defined serum-free media: growth-supporting substances. III. Ethanolamine, vitamins, and other low molecular weight nutrients.
The effects of two low molecular weight substances, ethanolamine and ascorbic acid, on growth of hybridomas in serum-free medium were studied. The optimum growth-supporting concentration of ethanolamine was 20 microM. Omission of ethanolamine led to significantly decreased growth of hybridoma cells (41-75% of the cells growing 3 days with ethanolamine). The optimum growth-supporting concentration of ascorbic acid was 3 micrograms/ml. Omission of ascorbic acid also resulted in decreased growth (60-80% of the cells growing 3 days with ascorbic acid). Other low molecular weight substances, such as 2-mercaptoethanol, putrescine, alpha-tocopherol, and retinal, had no growth-supporting effect.
D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004983:Ethanolamines / Q000494:pharmacology*; D006825:Hybridomas / Q000166:cytology*; D008623:Mercaptoethanol / Q000494:pharmacology; D011700:Putrescine / Q000494:pharmacology; D012172:Retinaldehyde / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
D003470:Culture Media; D004983:Ethanolamines; D014815:Vitamins; D014810:Vitamin E; D008623:Mercaptoethanol; D001205:Ascorbic Acid; D012172:Retinaldehyde; D011700:Putrescine