Nontransferrin-bound serum iron in thalassemia and sickle cell patients.
Nontransferrin-bound iron (NTBI) was separated from transferrin bound iron (TBI) by DEAE-Sephadex-CDS filtration. TBI is eluted with Tris-NaCl buffer, NTBI that is retained on the column is eluted with citric acid. NTBI was identified in serum from thalassemia and sickle cell patients. Normal serum contained less than 6% NTBI as compared with 15-18% in patient's sera. NTBI levels were decreased significantly after 8 hr chelation with deferoxamine (DFO).
D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood* / Q000188:drug therapy; D002852:Chromatography, Ion Exchange; D003676:Deferoxamine / Q000627:therapeutic use; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007501:Iron / Q000097:blood*; D011485:Protein Binding; D013789:Thalassemia / Q000097:blood* / Q000188:drug therapy; D014168:Transferrin / Q000378:metabolism
D014168:Transferrin; D007501:Iron; D003676:Deferoxamine