Preparation and evaluation of hemoglobin-polyethylene glycol conjugate (pyridoxalated polyethylene glycol hemoglobin) as an oxygen-carrying resuscitation fluid.
Human hemoglobin was pyridoxalated and subsequently coupled with the activated ester of polyethylene glycol (PEG) to provide an oxygen-carrying resuscitation fluid. The PEG conjugate of pyridoxalated hemoglobin (pyridoxalated PEG hemoglobin) has a longer half-life in the circulation (12.8 h) than does hemoglobin (2.2 h) when the solutions are exchange-transfused by approximately 60%. The P50 of pyridoxalated PEG hemoglobin, which is the partial pressure of oxygen at which one-half of the absorbed oxygen is released, was 21.3 +/- 1.4 mm Hg (mean +/- SD, n = 5) (37 degrees C, pH = 7.4), which is close to that of human whole blood (25.9 +/- 0.6 mm Hg). The colloidal osmotic pressure and the viscosity were 36.5 +/- 2.4 mm Hg (n = 5) and 2.6 +/- 0.4 cp (n = 5), respectively. The results indicate that the pyridoxalated PEG hemoglobin solution has favorable properties for use as an oxygen-carrying resuscitation fluid.
D000818:Animals; D004952:Esters; D006207:Half-Life; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008970:Molecular Weight; D010100:Oxygen / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000378:metabolism*; D011730:Pyridoxal / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012151:Resuscitation; D012212:Rheology; D013388:Succinimides / Q000138:chemical synthesis
D004952:Esters; D006454:Hemoglobins; D013388:Succinimides; D011730:Pyridoxal; D011092:Polyethylene Glycols; C050761:polyethylene glycol bis(succinimidyl succinate); D010100:Oxygen