title: A soluble factor (less than 4000 Da) from chick spinal cord blocks slow hyperpolarizing afterpotentials in cultured rat muscle cells.
abstract: The slow hyperpolarizing afterpotential (slow HAP) following an evoked action potential in cultured rat muscle cells is inhibited by coculture with spinal cord neurons, spinal cord-conditioned medium (CM) and by a low-molecular-weight fraction (less than 4000 Da) prepared from this CM. The incidence, amplitude and duration of the slow HAPs decreased significantly after 24 h of incubation. In contrast, chick retinal neuron cocultures or CM did not alter the slow HAP.
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002645:Chickens; D003864:Depression, Chemical; D009132:Muscles / Q000187:drug effects*; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000032:analysis; D013116:Spinal Cord / Q000032:analysis*
chemical_list: D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins