title: Multiple forms of acid phosphatase and their differential secretion during stalk formation under submerged monolayer incubation of Dictyostelium discoideum.
abstract: The electrophoretic pattern of intracellular and secreted acid phosphatases (AcPases) in Dictyostelium discoideum was examined during incubation of the cells as a submerged monolayer. Three distinct forms of the enzyme were observed in the cell during differentiation; one was detected throughout development (AcPase 1), whereas the others including AcPase 2 were stage-specific. AcPase 1 was released in the medium predominantly in early development and AcPase 2, a prestalk specific form, was secreted during stalk formation. When cells were incubated under conditions where stalk cells did not form, only AcPase 1 was recognized both in the cell and in the medium.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D002454:Cell Differentiation; D004023:Dictyostelium / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D000135:Acid Phosphatase