title: [Interaction of the fluorescent probe diS-C3-(5) with lecithin-cholesterol membranes].
abstract: Behaviour of fluorescent carbocyanine probe disS-C3(5) in the egg lecithin-cholesterol membrane suspension was studied in relation to the lecithin/cholesterol ratio. The partition coefficient of the probe between aqueous and lipid phases decreases unlinearly with increase of cholesterol molar part in a bilayer. This parameter over molar part units was estimated to be (2.4 +/- 0.1) X 10(6) for egg lecithin membranes and (1.8 +/- 0.2) X 10(6) for 10 mol% cholesterol, (1.2 +/- 0.1) X 10(6) for 20, (0.8 +/- 0.1) X 10(6) for 30, and (0.48 +/- 0.02) X 10(6) for 50 mol% cholesterol. It is suggested that the probe partition coefficient value consists of two components: one caused by pure lecithin bilayer regions and another by local lecithin concentration fluctuations in the mixed lecithin-cholesterol regions.
mesh_terms: D002784:Cholesterol; D005456:Fluorescent Dyes; D008081:Liposomes; D010713:Phosphatidylcholines
chemical_list: D005456:Fluorescent Dyes; D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol
vernacular_title: Vzaimodeĭstvie fluorestsentnogo zonda diS-C3-(5) s letsitin-kholesterinovymi membranami.