Detection of antibodies to hepatitis B surface antigen (HBsAg) using monoclonal antibody and the avidin-biotin system.
A direct ELISA using biotinylated HBsAg and a competitive ELISA using biotinylated monoclonal antibody were developed for the detection of antibodies to HBsAg. Both tests are capable of detecting 0.1 I.U. of anti-HBsAg antibody/ml. The direct ELISA was compared with a SPRIA test for anti-HBsAg antibody in human sera.
D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006801:Humans; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D001360:Avidin; D001710:Biotin