Separation of viroid RNAs by cellulose chromatography indicating conformational distinctions.
A series of viroids and viroid-like RNAs extracted from citron (Citrus medica), grapevine (Vitis vinifera), and avocado (Persea americanum) displayed differential binding capacities to cellulose in the presence of ethanol. This nonionic interaction was influenced by the presence of magnesium ions suggesting variations in conformation among the viroid RNAs and corresponding differences in reactivity with cellulose. The specific elution profiles present a basis for the subclassification of groups of viroid-like molecules.
D002482:Cellulose; D002845:Chromatography / Q000379:methods; D000431:Ethanol; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000302:isolation & purification*; D014772:Viroids / Q000032:analysis*
D012367:RNA, Viral; D000431:Ethanol; D002482:Cellulose