Ethanol impairs biliary lysosomal enzyme release in rats.
The effects of ethanol on hepatic lysosomes are poorly documented. This study examined the biliary release of lysosomal enzymes, a marker of the hepatocyte-to-bile excretory pathway, after ethanol administration in the isolated perfused rat liver model. At concentrations similar to those reached in human plasma during social drinking, ethanol markedly decreased biliary lysosomal enzyme output and bile flow in the rat. Ethanol did not affect hepatic activities or the release into perfusate of lysosomal and other subcellular marker enzymes. Hence, ethanol may potentially inhibit hepatocyte-to-bile excretion of other compounds processed through lysosomes.
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000201:enzymology*; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D000431:Ethanol; D007770:L-Lactate Dehydrogenase; D006596:Hexosaminidases; D005966:Glucuronidase; D000118:Acetylglucosaminidase