A simplified methodology for purification of peanut (Arachis hypogaea) agglutinin.
The agglutinin from peanut (Arachis hypogaea) was readily isolated by affinity chromatography on acid-treated Sepharose 6B. The recovered lectin (50 mg/100 g seeds) appeared as a single band of Mr 32,000 on gel electrophoresis and its specific haemagglutination titre on desialylated human A red blood cells was very high (2(15)).
D002846:Chromatography, Affinity / Q000379:methods; D037102:Lectins / Q000302:isolation & purification*; D008970:Molecular Weight; D019887:Peanut Agglutinin; D012685:Sepharose
D037102:Lectins; D019887:Peanut Agglutinin; D012685:Sepharose