NMR study of the interaction of P-450 with 4-methoxypyridine.
Longitudinal relaxation (T1) measurements for all lines (alpha-CH, beta-CH, O-CH3) in the 4-methoxypyridine 1H-NMR spectrum were used to study the interaction of 4-methoxypyridine with purified microsomal cytochrome P-450 from livers of phenobarbital-treated rats. The paramagnetic contribution to the observed T1(-1) value was determined from its dependence on 4-methoxypyridine concentration. In the P-450-4-methoxypyridine complex the latter is oriented so that the nitrogen of pyridine is directed towards the Fe3+.
D000818:Animals; D002248:Carbon Monoxide / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008862:Microsomes, Liver / Q000201:enzymology; D011725:Pyridines / Q000378:metabolism*; D051381:Rats
D011725:Pyridines; C051376:4-methoxypyridine; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System