Ultrastructural changes in isolated peptidergic nerve terminals induced by digitonin permeabilization and K+ stimulation in the sinus gland of the crab, Cardisoma carnifex.
The preparation of isolated peptidergic nerve terminals from the sinus gland (a neurohemal organ) of the crab (Cardisoma carnifex) is described. In this species the nerve endings can have diameters up to 30 microns. They release neurosecretory material as judged by the decrease in the volumetric density of granules upon depolarization with potassium. Similar results were obtained after permeabilization of the nerve terminals with digitonin, but only in the presence of micromolar concentrations of calcium. This preparation should prove useful in correlating electrical events with other cellular processes involved in stimulus-secretion coupling.
D000818:Animals; D003386:Brachyura; D002463:Cell Membrane Permeability / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004072:Digitonin / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009411:Nerve Endings / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D009489:Neurosecretion; D009490:Neurosecretory Systems / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D011188:Potassium / Q000494:pharmacology
D004072:Digitonin; D011188:Potassium