Isolation and characterization of the intact gastrin precursor from a gastrinoma.
Antibodies to the extreme C-terminal region of human progastrin have been used to monitor the isolation of high-Mr immunoreactive material in a gastrinoma extract. Microsequence analysis of the product revealed amino acid residues in the first 18 positions corresponding to those predicted from the cDNA sequence for preprogastrin starting at position 22; the sequence and immunochemical data together allow the identification of this material as intact progastrin. Implications for gastrin biosynthesis are discussed.
D000595:Amino Acid Sequence; D005755:Gastrins / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis / Q000556:secondary; D011498:Protein Precursors / Q000302:isolation & purification*; D015043:Zollinger-Ellison Syndrome / Q000378:metabolism*
D005755:Gastrins; D011498:Protein Precursors; C013706:big gastrin