Gas-liquid chromatographic separation of enantiomers as their diastereomeric derivatives, illustrated with 2-methylbutyric acid.
A gas-liquid chromatographic method has been developed to separate on a small scale (acid samples as small as 2 mg) the enantiomers of 2-methylbutyric acid and to estimate a few per cent of one isomer as a contaminant in the other. Both the alpha-methylbenzyl ester and the alpha-methylbenzyl amide give a satisfactory resolution of the enantiomers. Chromatographic conditions in the order of importance are the final column temperature, the rate of temperature programming and the initial column temperature.
D002087:Butyrates / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D013237:Stereoisomerism; D013696:Temperature
D002087:Butyrates; C019475:2-methylbutanoic acid