Structure and properties of albumin Tokushima and its proteolytic processing by cathepsin B in vitro.
Albumin Tokushima is a Japanese genetic variant of human serum albumin. Two homozygous and 6 heterozygous subjects with this variant were found in a family. Albumin Tokushima was purified from sera of the homozygous subjects. Its amino acid composition and amino-terminal sequence were determined and compared with those of a normal serum albumin. Albumin Tokushima with the amino-terminal sequence of Arg-Gly-Val-Phe-His-Arg-Asp-Ala-His-Lys-Ser-Glu-Val-Ala-His-Arg-Phe-Lys- Asp- Leu-Gly-Glu-Glu-Asn-Phe was found to be the same abnormal proalbumin as proalbumin Lille (Abdo, Y. et al. (1981) FEBS Lett. 131, 286-288). The isoelectric points of albumin Tokushima were pH 4.70 and 4.90 as compared with pH 5.05 and 5.25 of a normal serum albumin. Albumin Tokushima was converted to normal serum albumin by purified cathepsin B in vitro. Albumin Tokushima can bind Ni2+ at 4 degrees C but binds little at 37 degrees C.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002401:Cathepsin B / Q000378:metabolism*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D011228:Prealbumin / Q000378:metabolism*; D012709:Serum Albumin / Q000235:genetics
D000596:Amino Acids; C044412:Pollibauer proalbumin; D011228:Prealbumin; D012709:Serum Albumin; D002401:Cathepsin B