title: Enzymatic characterization of Babesia bovis.
abstract: Agarose gel electrophoresis was used to identify metabolic enzymes in Babesia bovis and B. bigemina. Glutamate dehydrogenase, lactate dehydrogenase, glucose phosphate isomerase, and hexokinase were identified in B. bovis- and B. bigemina-infected erythrocytes and B. bovis merozoite preparations. A specific electrophoretic mobility was observed for each enzyme. Malate dehydrogenase, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and adenylate kinase were only detected in normal erythrocyte preparations. Inter-species, but not intra-species, variation was noted when comparing electrophoretograms of both species. Kinin-activating activity was not detected in B. bovis-infected erythrocyte or merozoite preparations at pH 4.2 or 7.6.
mesh_terms: D000263:Adenylate Kinase / Q000032:analysis / Q000097:blood; D000818:Animals; D001403:Babesia / Q000201:enzymology*; D002417:Cattle; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000201:enzymology / Q000469:parasitology; D004950:Esterases / Q000032:analysis / Q000097:blood; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000097:blood; D005969:Glutamate Dehydrogenase / Q000032:analysis*; D006593:Hexokinase / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis / Q000097:blood; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis / Q000097:blood
chemical_list: D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D006593:Hexokinase; D000263:Adenylate Kinase; D004950:Esterases; D005956:Glucose-6-Phosphate Isomerase