title: Chlortetracycline and the transmembrane potential of the inner membrane of plant mitochondria.
abstract: The oxidation of NADH or succinate by Jerusalem-artichoke (Helianthus tuberosus L.) mitochondria in the presence of chlortetracycline induced an increase in chlortetracycline fluorescence. Any treatment that prevented the formation of a transmembrane potential (as monitored by changes in safranine absorbance, A511-A533), e.g. uncoupling with carbonyl cyanide p-trifluoromethoxyphenylhydrazone, inhibition of dehydrogenase activity or electron transport, anaerobiosis or depletion of substrate, prevented the increase in chlortetracycline fluorescence or caused it to disappear. Changes in chlortetracycline fluorescence were always slower than changes in the safranine absorbance. The increase in chlortetracycline fluorescence caused by succinate oxidation had an excitation maximum at 393 nm, indicating that a Ca2+-chlortetracycline complex was involved. The increase in fluorescence was observed even in the presence of EDTA, which removes all external bivalent cations, indicating that internal Ca2+ is mobilized. Although NADH and succinate oxidations gave the same membrane potential and qualitatively had the same effect on chlortetracycline fluorescence, NADH oxidation caused a much larger (over 3-fold) increase in chlortetracycline fluorescence than did succinate oxidation. It is possible that this is connected with the Ca2+-dependence of NADH oxidation. In the presence of 2 mM external Ca2+, chlortetracycline collapsed the transmembrane potential and uncoupled succinate and duroquinone oxidation.
mesh_terms: D002118:Calcium / Q000494:pharmacology; D002751:Chlortetracycline / Q000494:pharmacology*; D006368:Helianthus / Q000378:metabolism*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010944:Plants / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
chemical_list: D013386:Succinates; D009243:NAD; D019802:Succinic Acid; D002118:Calcium; D002751:Chlortetracycline