title: Vaccination of mice against malaria with soluble antigens. I. The effect of detergent, route of injection, and adjuvant.
abstract: Mice were vaccinated against lethal blood stage P. yoelii malaria with lysates of parasitised blood prepared in various detergents. Two injections, containing 25 micrograms of protein, of a Triton X-100 lysate gave optimal protection when injected intraperitoneally with saponin as adjuvant. Such lysates provide a starting point for the isolation of potential antigens for vaccination, selected for their protective activity in vivo.
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D003902:Detergents / Q000494:pharmacology; D005260:Female; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008288:Malaria / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010961:Plasmodium / Q000276:immunology*; D012503:Saponins / Q000494:pharmacology; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D000276:Adjuvants, Immunologic; D000953:Antigens, Protozoan; D003902:Detergents; D012503:Saponins; D014612:Vaccines