Crystallization and preliminary X-ray diffraction study of an endoglucanase from Clostridium thermocellum.
Endoglucanase D, a cellulose degradation enzyme from Clostridium thermocellum has been cloned in Escherichia coli, purified and crystallized. The crystals are trigonal, space group P3(1)12 (or P3(2)12) with a = 57.7 (+/- 0.1) A, c = 192.1 (+/- 0.2) A, and diffract X-rays to a resolution of 2.8 A. They are suitable for a high-resolution X-ray diffraction analysis.
D002480:Cellulase / Q000302:isolation & purification*; D003013:Clostridium / Q000201:enzymology*; D003460:Crystallization; D014961:X-Ray Diffraction
D002480:Cellulase