Regulation of a human cardiac actin gene introduced into rat L6 myoblasts suggests a defect in their myogenic program.
The rat myogenic cell line L6E9 induces skeletal but not cardiac alpha-actin mRNA upon fusion to form myotubes. However, when a human cardiac alpha-actin gene was introduced into L6E9 myoblasts, differentiation of the cells led to the accumulation of human gene transcripts in parallel with those derived from the endogenous skeletal alpha-actin gene. This result demonstrates that factors which direct rat myogenesis can regulate a muscle gene from another species and that the L6E9 cells may have a defect in their ability to activate endogenous cardiac actin gene expression.
D000199:Actins / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D005796:Genes; D005809:Genes, Regulator; D006801:Humans; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D000199:Actins; D012333:RNA, Messenger