title: Growth and differentiative maturation of the rat enterocyte.
abstract: Cells of the intestinal mucosa of the infant and adult rat maintain a pattern of continuous growth, accumulating structural and functional proteins and lipids while migrating the length of the villus column. Cells of jejunal and ileal segments were fractionated sequentially from villus tip to inner crypt and distribution patterns were determined for DNA, total protein, cholesterol, phospholipid, and disaccharidases. Patterns of increasing ratios of protein, lipids, and disaccharidases to DNA were maintained to villus tips, with only slight fall-off of enzymes observed. Distribution profiles of disaccharidases, when computed relative to protein (as seen in previous reports), show distortion of the true cellular distribution pattern of these enzymes as determined by the DNA content of the fractions. Wide variation in cell protein concentrations was evident between jejunal and ileal segments in pre- and postweaned rats. Ileal cells of the suckling rat contained particularly high protein concentrations, which appeared to be largely transitory in nature and related to food intake. Cholesterol and phospholipids were found to be concentrated in the microvillus membrane and account for a significantly large fraction of the cellular content of these lipids.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002784:Cholesterol / Q000378:metabolism; D004186:Disaccharidases / Q000378:metabolism; D007082:Ileum / Q000166:cytology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D007583:Jejunum / Q000166:cytology / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014886:Weaning
chemical_list: D010743:Phospholipids; D011506:Proteins; D002784:Cholesterol; D004186:Disaccharidases