The monomethylethanolamine- and dimethylethanolamine-base exchange reactions of rat-brain microsomal fraction.
The ability of crude rat-brain microsome preparations to convert DME and MME to their corresponding phospholipid was explored. In common with the other base-exchange reactions, the incorporations of DME and MME were stimulated by about 1-4 mM Ca2+, possessed slightly alkaline pH optima, were energy independent and were unaffected by exogenous phospholipids. The Km values were 0.97 mM and 0.5 mM and the Vmax values were 9.6 nmol/mg protein per h and 6.25 nmol/mg protein per h for DME and MME, respectively. The P3 fraction of the brain and heart had the highest specific activities of particles prepared from several tissues.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002250:Carbon Radioisotopes; D003642:Deanol / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism*; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism*; D051381:Rats; D014316:Tritium / Q000378:metabolism
D002250:Carbon Radioisotopes; D004983:Ethanolamines; D014316:Tritium; D003642:Deanol; D002118:Calcium; C017500:N-methylaminoethanol