Ornithine decarboxylase and spermidine/spermine N1-acetyltransferase are induced in K562 cells by S-adenosylmethionine decarboxylase inhibitor methylglyoxal bis(guanylhydrazone) but not by analogous methylglyoxal bis(butylamidinohydrazone).
The activities of ornithine decarboxylase (ODC) and spermidine/spermine N1-acetyltransferase (SAT) were increased by the addition of S-adenosylmethionine decarboxylase (AdoMetDC) inhibitor methylglyoxal bis(guanylhydrazone) (MGBG) in cultured human erythroid leukemia K 562 cells. ODC activity began to increase 4 hr after the addition of the drug and attained a maximum at 12 hr. The increase of SAT activity lagged behind that of ODC activity. The increases of both ODC and SAT activities produced by MGBG were blocked by treatment with cycloheximide, suggesting that the increase of enzyme activity resulted from the synthesis of new enzyme proteins. The putrescine content in cells treated with MGBG increased markedly, whereas the levels of spermidine and spermine were depressed lower. On the other hand, methylglyoxal bis(butylamidinohydrazone) (MGBB), a derivative of MGBG inhibiting AdoMetDC effectively, did not induce ODC or SAT activities.
D000123:Acetyltransferases / Q000096:biosynthesis*; D012437:Adenosylmethionine Decarboxylase / Q000037:antagonists & inhibitors*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002460:Cell Line; D004790:Enzyme Induction; D006801:Humans; D008935:Mitoguazone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D011073:Polyamines / Q000096:biosynthesis; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D012437:Adenosylmethionine Decarboxylase; D002262:Carboxy-Lyases; D008935:Mitoguazone; D009955:Ornithine Decarboxylase; D011073:Polyamines; D000099228:Diamine N-Acetyltransferase; C047167:methylglyoxal bis(butylamidinohydrazone)