title: Adherence of Bordetella avium to turkey tracheal mucosa: effects of culture conditions.
abstract: Adherence of Bordetella avium to the tracheal mucosa of turkeys was evaluated, using bacteria grown under different culture conditions. Several solid and liquid media were used at incubation times of 12, 24, 36, or 48 hours with incubation temperatures of 18, 26.5, or 35 C. Adherence of B avium was greatest when the bacteria were grown on solid media at 35 C. Use of Bordet-Gengou or brain-heart infusion agar was associated with significantly greater (P less than 0.05) adherence compared with adherence of bacteria grown on other media. Adherence was greatest, using cultures in the stationary phase of growth; however, with some media, adherence diminished when incubation was extended beyond 36 hours. Adherence of B avium was reduced but not completely prevented when cultures were incubated at 18 C.
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001884:Bordetella / Q000254:growth & development / Q000378:metabolism*; D001885:Bordetella Infections / Q000382:microbiology / Q000662:veterinary*; D003470:Culture Media; D009092:Mucous Membrane / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology*; D013696:Temperature; D014132:Trachea / Q000382:microbiology*; D014422:Turkeys
chemical_list: D003470:Culture Media