Precipitation of serum proteins by the lectin from wheat-germ.
We investigated the composition of the precipitate that forms when wheat-germ lectin derived from Triticum vulgaris is added to serum. A number of serum proteins are precipitated, representing about 2.5% of the total serum protein. This study demonstrates that the interaction of this lectin with the bone isoenzyme of alkaline phosphatase is not specific.
D000469:Alkaline Phosphatase / Q000032:analysis; D001798:Blood Proteins / Q000302:isolation & purification*; D001842:Bone and Bones / Q000201:enzymology; D011232:Chemical Precipitation; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D012965:Sodium Chloride; D014909:Wheat Germ Agglutinins
D001798:Blood Proteins; D007527:Isoenzymes; D014909:Wheat Germ Agglutinins; D012965:Sodium Chloride; D000469:Alkaline Phosphatase