Non-enzymic glycation of proteins: analysis of N-(1-deoxyhexitol-1-yl)amino acids by high-performance liquid chromatography.
A method for determining the extent of non-enzymic glycation (originally called "glycosylation") of both lysyl and N-terminal residues of a protein is described. The glycated protein is treated with sodium borohydride, and is then subjected to acid-catalysed hydrolysis. The resulting N-(1-deoxy-D-hexitol-1-yl)amino acids are separated by cation-exchange high-performance liquid chromatography (l.c.), and detected by a post-column reaction with periodate. The method has been applied successfully to samples of human hemoglobin and human serum albumin, for measurement of numbers of valine-attached and of lysine-attached N-(1-deoxy-D-fructos-1-yl) groups per protein molecule.
D000596:Amino Acids; D001894:Borohydrides; D002241:Carbohydrates; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006023:Glycoproteins / Q000138:chemical synthesis*; D006454:Hemoglobins; D006801:Humans; D006868:Hydrolysis; D012709:Serum Albumin
D000596:Amino Acids; D001894:Borohydrides; D002241:Carbohydrates; D006023:Glycoproteins; D006454:Hemoglobins; D012709:Serum Albumin