Testosterone metabolite profiles reveal differences in the spectrum of cytochrome P-450 isozymes induced by phenobarbitone, 2-acetylaminofluorene and 3-methylcholanthrene in the chick embryo liver.
The regiospecificity and stereoselectivity of testosterone hydroxylation by hepatic microsomes prepared from control, PB, 3MC and 2AAF treated chick embryos has been analysed. Microsomes prepared from control animals hydroxylate testosterone at the 16 alpha and 6 beta positions exclusively: 3MC treatment only causes comparatively minor alterations in the rates of these conversions. PB and 2AAF treatment induced 16 beta-hydroxylation, whilst only 2AAF caused a substantial induction of 6 beta hydroxylation. This data suggests that in the chick, 2AAF not only induces P-450 subforms which are also induced by PB but additional subforms which are not markedly induced by either PB or 3MC.
D015073:2-Acetylaminofluorene / Q000494:pharmacology*; D000818:Animals; D002642:Chick Embryo; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D006900:Hydroxylation; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008748:Methylcholanthrene / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology*; D013739:Testosterone / Q000378:metabolism*
D007527:Isoenzymes; D013739:Testosterone; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D015073:2-Acetylaminofluorene; D010634:Phenobarbital