An improved incubation apparatus for Western blots used for the detection of antinuclear antibodies.
To facilitate the use of Western blots for the detection of antibodies, we have developed an incubation apparatus. The use of this apparatus simplifies the incubation of blots with antisera, permits the testing of large numbers of sera and eliminates artefacts caused by the use of loose strips. The introduction of a pressure bag in the lower lid of the apparatus secures a steady pressure over the entire blot, a feature lacking in currently available commercial equipment. The detection of antinuclear antibodies is presented as an example of the use of this incubator.
D000936:Antigen-Antibody Complex / Q000032:analysis; D034961:Antigens, Nuclear; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007202:Indicators and Reagents; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009698:Nucleoproteins / Q000276:immunology*; D012016:Reference Values; D012595:Scleroderma, Systemic / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology
D000936:Antigen-Antibody Complex; D034961:Antigens, Nuclear; D001323:Autoantibodies; D001324:Autoantigens; D007202:Indicators and Reagents; D009698:Nucleoproteins