Changes in plasma enzyme concentrations in response to blood substitution with perfluorocarbon emulsion in the conscious rat.
The effects of near total blood replacement with the proprietary perfluorocarbon (PFC)-based emulsion, Fluosol-DA 20%, on plasma concentrations of 2 enzymes, lactate dehydrogeanse (LDH) and alkaline phosphatase (ALP), have been examined in conscious, chronically catheterized rats. A pronounced fall in both plasma LDH (p less than 0.05) an ALP (p less than 0.01) occurred in response to exchange-transfusion. However, at 6 h following blood replacement, plasma concentrations of both enzymes had risen to values significantly greater than those measured immediately before perfusion. The observed changes in plasma LDH and ALP after blood replacement with Fluosol-DA indicated alterations in normal functioning of tissues from which these enzymes originate.
D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001802:Blood Substitutes / Q000494:pharmacology*; D004338:Drug Combinations / Q000494:pharmacology; D005260:Female; D005466:Fluorocarbons / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D006400:Hematocrit; D006895:Hydroxyethyl Starch Derivatives; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D012119:Respiration / Q000187:drug effects
D001802:Blood Substitutes; D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase