Scanning electron microscopic studies of megakaryocytes and platelet formation in the dog and rat.
Megakaryocyte morphology and platelet formation in canine and murine bone marrows were studied by scanning electron microscopy. In situ-fixed bone marrow preparations and cell suspensions of bone marrow provided complementary information for the 2 species (dogs and rats). Cylindrical processes (proplatelets) of variable length and thickness, originating from the megakaryocyte surface, were in the larger marrow sinusoids and the central vein. Regional constrictions along the length of proplatelets, particularly near their apical region, and the presence of fragments of such processes supported the concept of platelet formation through segmentation of proplatelets. Megakaryocytes presented varied morphology. Surface features resembling platelets were observed on megakaryocytes, indicating that platelets may have been released through surface budding. In conclusion, megakaryocytes formed long proplatelet processes that actively migrated to venous sinusoids to release platelets by fragmentation. Scanning electron microscopy analysis revealed a complex and variable megakaryocyte surface topography. The platelet-like structures on megakaryocyte surfaces may represent platelet release by a budding mechanism. The similarity between murine platelet release and canine platelet release demonstrates that data from rodent models may be applicable to nonrodents.
D000818:Animals; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D001853:Bone Marrow / Q000502:physiology / Q000648:ultrastructure; D004285:Dogs; D008297:Male; D008533:Megakaryocytes / Q000502:physiology / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains