Effect of pollen extract on the development of experimental atherosclerosis in rabbits.
Our previous studies have shown that the pollen extract, Cernitin, reveals lipid-lowering properties in animals and humans. The present study was designed to investigate the influence of Cernitin on the development of experimental atherosclerosis in rabbits over a period of 12 weeks. Forty male mongrel rabbits were divided into 4 equal groups: (1) controls, (2) animals receiving high-fat diet (HFD) containing cholesterol and coconut oil, (3) HFD + pollen extract, and (4) HFD + clofibrate. The most pronounced reduction in lipid metabolism and in the severity of plaque formation occurred after the pollen extract had been applied. The total cholesterol content in serum and liver homogenate was depressed by 67% and 45%, respectively, while the serum HDL cholesterol and alpha-lipoproteins level was increased by 19% and from 7.73% to 21.73% respectively. The cytochrome P-450 content in the liver microsomes was elevated by 98% (nmol/g liver). Atherosclerotic plaque intensity at 12 weeks, measured planimetrically, averaged 85.5% in HFD-fed animals vs 33.7% in pollen extract-treated rabbits. These findings suggest that Cernitin, in addition to significantly lowering serum lipid levels in rabbits on an experimental diet, may modify lipid deposition in major arteries.
D000818:Animals; D001161:Arteriosclerosis / Q000097:blood / Q000473:pathology* / Q000517:prevention & control; D001835:Body Weight / Q000187:drug effects; D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL / Q000097:blood; D002994:Clofibrate / Q000627:therapeutic use; D004036:Diet, Atherogenic; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008099:Liver / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts; D011058:Pollen / Q000627:therapeutic use*; D011817:Rabbits; D012434:Secale
D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D008055:Lipids; D008077:Lipoproteins, LDL; D010936:Plant Extracts; C010070:cernilton; D002994:Clofibrate