Adaptation of Pseudomonas putida mt-2 to growth on aromatic amines.
Pseudomonas putida mt-2 (ATCC 33015) carrying the TOL plasmid pWW0 could adapt to growth on the aromatic amines aniline and m- and p-toluidine. In strain UCC2, a derivative adapted to rapid growth on these compounds, they were oxidatively deaminated to catechol or 4-methylcatechol, which in turn were dissimilated by a meta-cleavage pathway. The aniline/toluidine oxygenase and the meta-cleavage pathway enzymes were inducible by aromatic amines. Evidence is presented that in strain UCC2, plasmid pWW0 has undergone deletion of its catabolic genes, and that it is a novel plasmid, pTDN1, which is involved in the catabolism of aniline and m- and p-toluidine. The meta-cleavage pathway genes which are carried by pTDN1 were shown not to have originated in pWW0.
D000222:Adaptation, Physiological; D000588:Amines / Q000378:metabolism*; D001673:Biodegradation, Environmental; D010957:Plasmids; D011549:Pseudomonas / Q000254:growth & development*; D013379:Substrate Specificity; D014050:Toluene / Q000378:metabolism; D014052:Toluidines / Q000378:metabolism
D000588:Amines; D014052:Toluidines; D014050:Toluene