Light-modulated ADP-ribosylation, protein phosphorylation and protein binding in isolated fly photoreceptor membranes.
Rhodopsin (P, lambda max 480 nm) of blowfly photoreceptors R1-6 is converted by light into a thermally stable metarhodopsin (M, lambda max 565 nm). In isolated blowfly rhabdoms photoconversion of P to M affects bacterial toxin-catalyzed ADP-ribosylation of a 41-kDa protein, activates phosphorylation of opsin and induces the binding of a 48-kDa phosphoprotein to the rhabdomeric membrane. ADP-ribosylation of the 41-kDa protein is catalyzed by cholera toxin and is inhibited by P----M conversion. The 41-kDa protein might represent the alpha-subunit of the G-protein, proposed to be part of the phototransduction mechanism [Blumenfeld, A. et al. (1985) Proc. Natl Acad. Sci. USA 82, 7116-7120]. P----M conversion leads to phosphorylation of opsin at multiple binding sites: up to 4 mol phosphate are bound/mol M formed. Dephosphorylation of the phosphate binding sites is induced by photoconversion of M to P. High levels of calcium (2 mM) inhibit phosphorylation of M and increase dephosphorylation of P. Protein patterns obtained by sodium dodecyl sulfate gel electrophoresis of irradiated retina membranes show an increased incorporation of label from [gamma-32P]ATP also into protein bands of 48 kDa, 68 kDa and 200 kDa. Binding studies reveal that in the case of the 48-kDa protein this effect is primarily due to a light-induced binding of the protein to the photoreceptor membrane. The binding of the 48-kDa phosphoprotein is reversible: after M----P conversion the protein becomes extractable by isotonic buffers. These data suggest that in rhabdomeric photoreceptors of invertebrates light-activation of rhodopsin is coupled to an enzyme cascade in a similar way as in the ciliary photoreceptors of vertebrates, although there may be differences, e.g. in the type of G-protein which mediates between the activated state of metarhodopsin and a signal-amplifying enzyme reaction.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D002118:Calcium / Q000494:pharmacology; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D004175:Diptera; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D008565:Membrane Proteins / Q000302:isolation & purification; D010766:Phosphorylation; D010777:Photochemistry; D010786:Photoreceptor Cells / Q000502:physiology*; D011485:Protein Binding; D012243:Rhodopsin / Q000378:metabolism
D008565:Membrane Proteins; D000246:Adenosine Diphosphate Ribose; D012243:Rhodopsin; D002118:Calcium