Expression of the cloned S4 gene of reovirus serotype 3 in transformed eucaryotic cells: enrichment of the viral protein in the crude initiation factor fraction.
The sigma 3 protein of reovirus is believed to play a role in the control of protein synthesis in reovirus-infected cells. In this paper we describe the establishment of a line of L-cells expressing the sigma 3 protein from the cloned S4 gene of reovirus serotype 3, under the control of the SV40 early promoter. The protein was enriched in the crude initiation factor fraction prepared by a high-salt wash of ribosomes. There was no apparent detrimental effect on the cell line.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005814:Genes, Viral; D012089:Mammalian orthoreovirus 3 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009693:Nucleic Acid Hybridization; D010448:Peptide Initiation Factors / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012087:Reoviridae / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D010448:Peptide Initiation Factors; D014764:Viral Proteins