Estrogen participation in induction of cervicovaginal adenosis-like lesions in immature mice exposed prenatally to diethylstilbestrol.
A high incidence of vaginal adenosis-like lesions (ADL, 67%) was found in 30-day-old offspring of ICR/JCL mice given 4 daily subcutaneous injections of 2,000 micrograms diethylstilbestrol (DES) on days 15-18 of gestation. The prenatally DES-exposed mice and the controls ovariectomized at 10 days of age were given 5 daily subcutaneous injections of 10(-4), 10(-3), 10(-2), 10(-1), or 1 micrograms 17 beta-estradiol (E2), respectively, starting at 25 days of age. ADL was never observed in the age-matched controls given prepubertal injections of any E2 dose. In contrast, high incidences of ADL (63-100%) were found in the vaginal fornix and upper vagina of 30-day-old DES mice receiving prepubertal injections of 10(-3)-1 micrograms E2/day, whereas in DES mice given prepubertal injections of the vehicle alone and a lower daily dose of 10(-4) micrograms E2, incidences were nil and 25%, respectively. Mitotic figures were found in the columnar epithelium with ADL, although the rate was lower than in the ADL-freed stratified epithelium. It is suggested, therefore, that an ovarian hormone (probably estrogen) participates in the prepubertal induction of ADL in DES mice.
D000818:Animals; D002584:Cervix Uteri / Q000473:pathology; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004848:Epithelium / Q000473:pathology; D004967:Estrogens / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D007267:Injections; D051379:Mice; D008813:Mice, Inbred ICR; D010052:Ovariectomy; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D002577:Uterine Cervical Diseases / Q000139:chemically induced* / Q000473:pathology; D014621:Vagina / Q000473:pathology; D014623:Vaginal Diseases / Q000139:chemically induced* / Q000473:pathology
D004967:Estrogens; D004054:Diethylstilbestrol