title: Iron mobilization from isolated hepatocytes.
abstract: It is not known which message and mechanism triggers the cell to mobilize iron from ferritin. In this paper we present the results of incubation experiments with 59Fe-labelled hepatocytes. Anemic serum gives a significant higher rate of iron mobilization than normal serum. The involvement of apo-transferrin is ruled out because it did not increase iron mobilization. Citrate increased iron mobilization which is not the result of an increase in NADH/NAD+-ratio because addition of ethanol did not stimulate iron mobilization. Desferrioxamine is used clinically in iron overloaded patients and it is known that iron removal is a very slow process. Although desferrioxamine can mobilize iron from ferritin in hepatocytes, a considerable amount remains inside the cell as a low molecular weight fraction. This fraction represents chelator bound iron and is slowly released into the circulation.
mesh_terms: D000740:Anemia / Q000097:blood; D000818:Animals; D001059:Apoproteins; D002951:Citrates / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism*; D007504:Iron Radioisotopes; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014168:Transferrin / Q000378:metabolism
chemical_list: D001059:Apoproteins; D002951:Citrates; D007504:Iron Radioisotopes; D014168:Transferrin; C028284:apotransferrin; D007501:Iron; D003676:Deferoxamine