title: Detection and identification of mutagens by the adducts formed upon reaction with guanosine derivatives.
abstract: Mutagens present in crude samples such as heated glucose can be detected or identified by means of the adducts formed upon reaction with a fluorescent guanosine derivative (FG) or isopropylideneguanosine (IPG). After the reaction of IPG with heated glucose, two adducts were isolated by high-performance liquid chromatography. One of the adducts was identified as the cyclic adduct formed between IPG and glyoxal. Mesoxaldialdehyde, which is structurally related to glyoxal, also produced a cyclic IPG-adduct and showed mutagenic activity in Salmonella typhimurium strain TA100. The other adduct isolated from the reaction mixture of IPG and heated glucose was 8-hydroxy-IPG. Various reagents which generate oxygen radicals were effective in the hydroxylation of guanosine derivatives at the C-8 position. These reagents also cause hydroxylation of guanine residues in DNA.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D005609:Free Radicals; D005947:Glucose; D006151:Guanosine / Q000031:analogs & derivatives*; D006358:Hot Temperature; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis / Q000378:metabolism*; D011804:Quinolines / Q000032:analysis
chemical_list: D005609:Free Radicals; D009153:Mutagens; D011804:Quinolines; D006151:Guanosine; D005947:Glucose