Salicylates and 12-lipoxygenase activity in human washed platelets.
In vitro salicylates /aspirin, salicylic acid, salicylamide and gentisic acid/ inhibited formation of 12-lipoxygenase products in intact human washed platelets which were stimulated with thrombin or arachidonic acid. Salicylates did not affect 12-lipoxygenase activity in platelet lysates. Ex vivo aspirin or salicylamide at a dose of 1 g given orally to healthy volunteers potentiated formation of 12-lipoxygenase products in washed platelets. It is concluded that the effect of salicylates on 12-lipoxygenase pathway is independent from their influence on cyclooxygenase activity in platelets and aspirin cannot be considered as a selective inhibitor of platelet cyclooxygenase.
D000328:Adult; D001092:Arachidonate 12-Lipoxygenase / Q000097:blood; D001091:Arachidonate Lipoxygenases / Q000037:antagonists & inhibitors*; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D005841:Gentisates; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology; D066298:In Vitro Techniques; D016859:Lipoxygenase Inhibitors; D012457:Salicylamides / Q000494:pharmacology; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid
D005841:Gentisates; D062385:Hydroxybenzoates; D016859:Lipoxygenase Inhibitors; D012457:Salicylamides; D012459:Salicylates; D001091:Arachidonate Lipoxygenases; D001092:Arachidonate 12-Lipoxygenase; C031060:salicylamide; D020156:Salicylic Acid; D001241:Aspirin; C010925:2,5-dihydroxybenzoic acid