Evaluation of a direct spectrophotometric method for the rapid determination of ascorbate and dehydroascorbate in blood using ascorbate oxidase.
The application of a technique for the direct spectrophotometric determination of ascorbate and dehydroascorbate in blood samples is described. The reliability of the test system and various disturbing factors are assessed. It is concluded that the technique possesses a high specificity and that interferences by various biochemicals and metabolic intermediates are low. The lowest ascorbate concentration detectable in plasma by this assay is estimated to be about 1 mumol/l.
D001204:Ascorbate Oxidase; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000097:blood*; D003683:Dehydroascorbic Acid / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D001204:Ascorbate Oxidase; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid