[Quantitative characteristics of the process of solubilizing hemagglutinin isolated from influenza viruses H1N1 and H3N2].
Quantitative characteristics of the process of solubilization of influenza H1N1 and H3N2 virus hemagglutinin treated with bromeline enzyme were studied. After a long-time contact of viruses with the enzyme the hemagglutinin was found to be destroyed significantly. Certain conditions should be observed in order to increase the amount of hemagglutinin separated by the enzyme and its final yield. First, the quality of the original virus concentrate should be evaluated by electron microscopy. Second, the process of hemagglutinin solubilization should be monitored by single radial immunodiffusion method and electron microscopy to determine the optimal period of incubation of the virus material with bromeline. Finally, several (usually 2-3) incubations of optimal duration ("fractionated" incubation) should be carried out.
D001963:Bromelains / Q000494:pharmacology; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000302:isolation & purification*; D053118:Influenza A Virus, H1N1 Subtype; D053122:Influenza A Virus, H3N2 Subtype; D009980:Influenza A virus / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification*; D008854:Microscopy, Electron; D012995:Solubility; D013997:Time Factors
D006389:Hemagglutinins, Viral; D001963:Bromelains
Izuchenie kolichestvennoĭ kharakteristiki protsessa soliubilizatsii gemaggliutinina pri ego vydelenii iz virusov grippa H1N1 i H3N2.