[Effect of CDDP on cultured glioma cell lines: analysis of cell kinetics by flow cytometry].
The cytokinetic response of cultured glioma cell lines to cis-dichlorodiammine platinum (II) (CDDP) was investigated by flow-cytometry using the BUdR-Hoechst technique. By this technique, the percentage of cells in the various components and the relative phase durations were calculated rapidly and simply. In the presence of CDDP ID50, perturbation of the cell cycle was shown and the cell cycle time was prolonged three times longer than in controls in the GB2 cell line. Comparison of histograms obtained at certain time intervals, cell-cycle perturbation by CDDP such as G2-block with prolongation of the S-phase and further G1 accumulation were demonstrated.
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D005434:Flow Cytometry; D005910:Glioma / Q000473:pathology*; D051381:Rats
D001973:Bromodeoxyuridine; D002945:Cisplatin