title: Isolation and identification of two protein-protein crosslinks within the two subunits of Bacillus stearothermophilus ribosomes.
abstract: Bacillus stearothermophilus 30S and 50S ribosomal subunits were isolated and crosslinked with diepoxybutane. The crosslinked proteins were extracted with LiCl or with 67% acetic acid and purified by a combination of different high performance liquid chromatography techniques. The protein fractions were analysed by two-dimensional and diagonal polyacrylamide gel electrophoresis and by immunological methods. Two crosslinked protein pairs, one from the large and one from the small subunit, consisting of proteins L23-L29 and S13-S19 respectively, were isolated in milligram amounts for determination of the crosslinked amino acids.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004852:Epoxy Compounds; D001411:Geobacillus stearothermophilus / Q000648:ultrastructure*; D046911:Macromolecular Substances; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000648:ultrastructure*
chemical_list: D003432:Cross-Linking Reagents; D004852:Epoxy Compounds; D046911:Macromolecular Substances; D012269:Ribosomal Proteins; C007366:diepoxybutane