[Growth and differentiation of cortical cells of the crystalline lens of the bovine fetus in tissue culture].
Cortical cells in the lens of the cattle fetuses in tissue culture migrate, proliferate and form growth zones of epithelioid, fibroblast-like and bubble-like cells. In contrast to differentiation, in the lens in vivo about 80-90% of epithelioid, 70-80% of fibroblast-like and 0.01% of bubble-like culture cells proliferate. Organells from the cultivated cells do not disappear. Cristallins are not revealed in most of the epithelioid and bubble-like cells, and in fibroblast-like ones--they are absent. In the cells of lentoids cristallins appear again. Proliferation, structural complication and disappearance of the cristalins can be considered as differentiation of the cortical cells under the cultivation conditions. Formation of cristallin-containing lentoids should be regarded as a phenomenon of secondary differentiation. Appearance in the culture of outgrowths of of epitheloid, fibroblast-like and bubble-like cells, that do not possess tissue-specific proteins, should be indicated as tissue potencies of the lens under the experimental conditions.
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002465:Cell Movement; D003459:Crystallins / Q000378:metabolism; D046508:Culture Techniques; D007908:Lens, Crystalline / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D008938:Mitosis
D003459:Crystallins
Rost i differentsirovka kortikal'nykh kletok khrustalika plodov krupnogo rogatogo skota v tkanevoĭ kul'ture.