Purification of porphobilinogen deaminase from human erythrocytes by fast protein liquid chromatography.
A four-step procedure using fast protein liquid chromatography is described for the isolation of porphobilinogen deaminase from human erythrocytes. The specific activity of the porphobilinogen deaminase is increased about 13,000-fold, and the preparation is electrophoretically pure on SDS-polyacrylamide gel electrophoresis.
D000642:Ammonia-Lyases / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000097:blood / Q000302:isolation & purification*
D011163:Hydroxymethylbilane Synthase; D000642:Ammonia-Lyases