[Relation of the poly(ADP-ribosylation) of proteins to the formation and repair of DNA single-strand breaks in gamma-irradiated permeable Zajdela hepatoma cells].
A study was made of the effect of poly(ADP-ribosylation) of proteins on the formation and repair of single-strand DNA breaks in gamma-irradiated (50 Gy) permeable Zajdela ascites hepatoma cells permeabilized by the treatment with 0.05% triton X-100. Incubation of gamma-irradiated permeable cells in conditions promoting DNA synthesis and providing ADP-ribosylation (in the presence of 1 mM NAD) did not cause any substantial changes in the formation of single-strand DNA breaks and did not influence their repair.
D000818:Animals; D002463:Cell Membrane Permeability; D003037:Cobalt Radioisotopes; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D005720:Gamma Rays; D066298:In Vitro Techniques; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011831:Radiation Genetics; D051381:Rats
D003037:Cobalt Radioisotopes; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose
Issledovanie vzaimosviazi poli(ADF-ribozilirovaniia) belkov s formirovaniem i reparatsieĭ odnonitevykh razryvov DNK v gamma-obluchennykh pronitsaemykh kletkakh gepatomy Zaĭdela.