[Collection and freezing of blood stem cells in acute nonlymphoblastic leukemia].
Blood-derived hemopoietic stem cells were collected using a continuous (10 patients) or a semi-continuous flow separator (2 patients) in some patients with acute non-lymphocytic leukemia. For ten out of those patients, five to seven leukaphereses were performed during a short period (median = 11 days) of marrow recovery following severe aplasia induced by an intensive chemotherapy. The mean number of CFU-GM cells collected per leukapheresis and per patient respectively was 6.7 X 10(4)/kg and 38.5 X 10(4)/kg. This latter number was similar to that obtained during a marrow harvest performed for a bone marrow transplantation, suggesting that high numbers of hemopoietic stem cells can be collected from the peripheral blood in leukemic patients and used for autologous transplantation.
D000208:Acute Disease; D000328:Adult; D001793:Blood Preservation / Q000379:methods*; D001800:Blood Specimen Collection / Q000379:methods*; D001853:Bone Marrow / Q000473:pathology; D005260:Female; D005615:Freezing; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis / Q000379:methods; D007938:Leukemia / Q000097:blood*; D008297:Male; D008875:Middle Aged
Collection et congélation des cellules souches sanguines au cours des leucémies aiguës non lymphoblastiques.