Effect of malonyl-CoA on delta 6 desaturation activity of rat liver microsomes.
The effect of malonyl-CoA on linoleic acid desaturation and elongation reactions of rat liver microsomes was studied. Under strict desaturation conditions, the in vitro microsomal conversion of linoleic acid to gamma-linolenic acid is time-dependent. When malonyl-CoA was added to the aforementioned incubation medium, linoleic acid was desaturated to gamma-linolenic acid and elongated to its higher homologues. Under these conditions, delta 6 desaturation activity, calculated by adding gamma-18:3, 20:3 and 20:4 acids, was neither inhibited nor activated by malonyl-CoA. These results indicate that the elongation of gamma-linolenyl-CoA coupled to the desaturation of linoleic acid did not modify delta 6 desaturase activity.
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000818:Animals; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008316:Malonyl Coenzyme A / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D000214:Acyl Coenzyme A; D008041:Linoleic Acids; D008316:Malonyl Coenzyme A; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases