title: Evaluation of three qualitative tests for detection of chloroquine in urine--agreement with plasma concentrations determined with liquid chromatography.
abstract: Three qualitative tests for detection of chloroquine in urine were compared and evaluated against concomitant plasma concentrations determined with liquid chromatography. All urine tests from five volunteers taking a single dose of 5 mg chloroquine base kg-1 were positive for at least ten days with the Haskins test, and for at least three days with the Wilson Edeson test. Even on the first day, the Dill-Glazko test indicated chloroquine in the urine of one volunteer only. After a single dose of 10 mg kg-1 to five other volunteers the Haskins test was uniformly positive for 14 days while the Wilson Edeson test was positive for four days. The Dill-Glazko test was positive for one day only. No false negative results were obtained with the Haskins test when plasma concentrations were above 0.03 mumol 1(-1), as compared to 0.11 mumol 1(-1) with the Wilson Edeson test. The Dill-Glazko test was uniformly positive when plasma concentrations were above 0.38 mumol 1(-1). The Dill-Glazko test was affected by low urinary pH and by addition of erythrocytes to urine. We conclude that the Dill-Glazko test should not be used.
mesh_terms: D002738:Chloroquine / Q000097:blood / Q000652:urine*; D002853:Chromatography, Liquid; D004912:Erythrocytes; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008288:Malaria / Q000652:urine; D008297:Male; D013997:Time Factors
chemical_list: D002738:Chloroquine