Purification and subunit structure of phosphoglycerate dehydrogenase from rabbit liver.
D-3-Phosphoglycerate dehydrogenase (EC 1.1.1.95) was purified from rabbit liver by (NH4)2SO4 fractionation, DEAE-Sephacel chromatography, affinity chromatography on AMP-agarose and molecular-sieve h.p.l.c. The purified enzyme was homogeneous as judged by SDS/polyacrylamide-slab-gel electrophoresis. On the basis of molecular-sieve h.p.l.c. and SDS/polyacrylamide-gel electrophoresis, the enzyme is a tetramer composed of subunits of Mr 60,000.
D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D050543:Phosphoglycerate Dehydrogenase; D011817:Rabbits
D046911:Macromolecular Substances; D002237:Carbohydrate Dehydrogenases; D050543:Phosphoglycerate Dehydrogenase