Detection of a new chloroperoxidase in Pseudomonas pyrrocinia.
A new chloroperoxidase could be detected in Pseudomonas pyrrocinia ATCC 15,958, a bacterium that produces the antifungal antibiotic pyrrolnitrin. This enzyme was separated from a ferriprotoporphyrin IX containing bromoperoxidase which was also produced by this bacterium. The enzyme is capable of catalyzing the chorination of indole to 7-chloroindole. This procaryotic chloroperoxidase requires the presence of H2O2 and can also brominate monochlorodimedone, but cannot catalyze its chlorination. This enzyme is the first chloroperoxidase described from procaryotic sources.
D002711:Chloride Peroxidase / Q000302:isolation & purification / Q000378:metabolism*; D002849:Chromatography, Gas; D007700:Kinetics; D013058:Mass Spectrometry; D010544:Peroxidases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*
D010544:Peroxidases; D002711:Chloride Peroxidase