Concentration and enzyme content of in vitro-cultured Babesia bigemina-infected erythrocytes.
Clones of in vitro-cultured Babesia bigemina-infected erythrocytes were concentrated by several density gradient procedures. The density range of infected erythrocytes containing pairs of parasites was 1.077 to 1.089 g/ml, whereas the density range of infected erythrocytes containing single parasites was 1.092 to 1.100 g/ml. Three enzymes--lactate dehydrogenase, glucose-phosphate isomerase, and glutamate dehydrogenase--were found associated with infected erythrocytes. The parasite-specific enzyme and/or isoenzymes were shown to have different mobility patterns in starch gel electrophoresis from those found in the normal bovine erythrocytes. The enzyme 6-phosphogluconate dehydrogenase was not detected as a parasite-specific enzyme in B. bigemina-infected erythrocytes.
D000818:Animals; D001403:Babesia / Q000201:enzymology*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D004592:Electrophoresis, Starch Gel; D004912:Erythrocytes / Q000201:enzymology / Q000469:parasitology*; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D005969:Glutamate Dehydrogenase / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase