A HPLC method for the simultaneous determination of disopyramide, lidocaine and their monodealkylated metabolites.
This paper describes a simple high-performance liquid chromatographic method for the determination of disopyramide and lidocaine simultaneously with their dealkylated metabolites. After basic tert-butyl methyl ether extraction and back-extraction with phosphoric acid, the drugs and their metabolites were injected into a Supelcosil CN column and the absorbance of the eluate was measured at 215 nm. The sensitivity was 0.3 mumol/l and the obtained precision, selectivity and stability during storage were adequate for the performed clinical studies in patients therapeutically treated with disopyramide and/or lidocaine. Some results from the clinical studies are briefly presented.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004206:Disopyramide / Q000097:blood*; D006801:Humans; D008012:Lidocaine / Q000097:blood*
D008012:Lidocaine; D004206:Disopyramide