Quasi-elastic laser light scattering from solutions and gels of hemoglobin S.
Quasi-elastic light scattering has been used to examine solutions and gels of deoxyhemoglobin S. The autocorrelation function is found to decay with a characteristic exponential relaxation which can be ascribed to the diffusion of monomer (64,000 molecular weight) hemoglobin S molecules. In the absence of polymers, the relaxation time is in good agreement with previous measurements of the diffusion coefficient for solutions of normal human hemoglobin. In the presence of the polymer phase, a large (greater than 200-fold) increase in the scattered intensity is observed but no contribution to the decay of the autocorrelation function from the motion of the aligned polymer phase can be detected. Heterodyning between the time-independent scattering amplitude from the polymers and the time-dependent scattering of the diffusing monomers results in a twofold increase in the relaxation time arising from monomer diffusion.
D004548:Elasticity; D005782:Gels; D006451:Hemoglobin, Sickle / Q000378:metabolism*; D006801:Humans; D007834:Lasers; D011487:Protein Conformation; D012542:Scattering, Radiation; D012996:Solutions; D013816:Thermodynamics
D005782:Gels; D006451:Hemoglobin, Sickle; D012996:Solutions