Myoglobin facilitated oxygen diffusion maintains mechanical function of mammalian cardiac muscle.
Myoglobin, an intracellular iron containing protein that binds oxygen reversibly, has been shown in model systems to facilitate the diffusion of oxygen and thereby maintain the mechanical function of exercising canine skeletal muscle and of hypoxic benthic fish hearts. Since no such role has yet been established for mammalian cardiac muscle small diameter (less than or equal to 0.70 mm) isolated kitten papillary muscles were stimulated at 24 X min-1 under isometric conditions in a physiological bath maintained at 30 degrees C with an oxygen tension of approximately equal to 450 mm Hg (59.8 kPa) to obtain a level of oxygenation just adequate to meet the metabolic needs of the muscles, as confirmed experimentally. Myoglobin was inactivated by adding 2 X 10(-3) mol X litre-1 sodium nitrite to the bath to abolish the facilitated diffusion of oxygen in the presence or absence of glycolytic blockade by 10(-4) mol X litre-1 sodium iodoacetate. This resulted in a 22(8)% (with blockade) or 10(3)% (without blockade) decrease (p less than 0.05) in the maximal rate of relaxation (-dT/dtmax) of the papillary muscles. Since the depression in mechanical function was reversible by increasing the bath oxygen tension to approximately equal to 600 mm Hg (79.8 kPa) it is concluded that the myoglobin facilitated diffusion of oxygen plays a role in maintaining the mechanical function of mammalian cardiac muscle under normal conditions. Furthermore, the maximal rate of relaxation of cardiac muscle is a sensitive indicator of the presence of hypoxia.
D000818:Animals; D002415:Cats; D004058:Diffusion; D004558:Electric Stimulation; D006019:Glycolysis / Q000187:drug effects; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D009211:Myoglobin / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010210:Papillary Muscles / Q000378:metabolism / Q000502:physiology*; D012977:Sodium Nitrite / Q000494:pharmacology
D007461:Iodoacetates; D009211:Myoglobin; D012977:Sodium Nitrite; D010100:Oxygen; D019807:Iodoacetic Acid