Reversed-phase high-performance liquid chromatographic separation of steroids with the beta-crotonate side chain.
An isocratic high-performance liquid chromatographic separation of some ethyl 3-stereoidyl crotonates [ethyl-24-nor-20(22)-cholen-23-oate derivatives] was developed. The separations were achieved by reversed-phase chromatography (Separon Si C18) using methanol, methanol-water, methanol-0.1 M formic acid and ethanol-0.01 M aqueous phosphoric acid mixtures as mobile phases. The steroidal crotonates were detected at 230 and 240 nm.
D002087:Butyrates / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003437:Crotonates / Q000302:isolation & purification*; D000432:Methanol; D013056:Spectrophotometry, Ultraviolet; D013256:Steroids / Q000302:isolation & purification*; D014867:Water
D002087:Butyrates; D003437:Crotonates; D013256:Steroids; D014867:Water; D000432:Methanol