Biosynthesis of Rhizobium trifolii capsular polysaccharide: enzymatic transfer of pyruvate substitutions into lipid-bound saccharide intermediates.
The activity of capsular polysaccharide pyruvyltransferase catalyzing the pyruvylation of acidic heteropolysaccharide was measured in Rhizobium trifolii 843 and 0403 rif. This enzyme activity was determined with EDTA-treated cells, uridine diphosphate-sugar precursors, and phosphoenol [1-14C]pyruvate. Activity was measured by the incorporation of radioactivity into organic solvent-soluble glycoconjugates. Enzymatic pyruvylation of capsular polysaccharide occurred from phosphoenolpyruvate at the lipid-bound saccharide stage.
D019880:Aldehyde-Ketone Transferases; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012231:Rhizobium / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism
D011135:Polysaccharides, Bacterial; D014539:Uridine Diphosphate Sugars; D010728:Phosphoenolpyruvate; D014166:Transferases; D019880:Aldehyde-Ketone Transferases; C050676:polysaccharide pyruvyltransferase