A perfusion technique for 13C NMR studies of the metabolism of 13C-labeled substrates by mammalian cells.
We describe an improved perfusion system designed for NMR studies with 13C-labeled metabolites. Using this system we have monitored the uptake and metabolism of [13C]acetate and [13C]glucose in pooled human lymphocytes cast in an agarose gel thread.
D000085:Acetates / Q000378:metabolism; D002247:Carbon Isotopes; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010477:Perfusion
D000085:Acetates; D002247:Carbon Isotopes; D005947:Glucose