Purification of human monocytes on gelatin-coated surfaces.
Human peripheral blood monocytes secrete a cell membrane-associated glycoprotein, cold insoluble globulin (fibronectin). Since fibronectin binds to gelatin-coated surface, we developed a simple technique for separation of human peripheral blood monocytes from whole mononuclear cell preparation. These preparations are characterized by a high monocyte purity (more than 90%), low platelet contamination and excellent viability.
D000954:Antigens, Surface / Q000032:analysis; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D005353:Fibronectins / Q000097:blood; D005780:Gelatin; D006801:Humans; D009000:Monocytes / Q000166:cytology*; D010641:Phenotype
D000954:Antigens, Surface; D005353:Fibronectins; D005780:Gelatin