The complete amino acid sequence of the B-chain of ricin E isolated from small-grain castor bean seeds. Ricin E is a gene recombination product of ricin D and Ricinus communis agglutinin.
The complete amino acid sequence of the B-chain of ricin E has been determined. The reduced and carboxymethylated B-chain was digested with trypsin, followed by separation and purification of the resulting peptides using reverse-phase HPLC. The amino acid sequence of each tryptic peptide was determined employing the DABITC/PITC double-coupling method. The B-chain of ricin E proved to consist of 262 amino acid residues. By comparing the amino acid sequence of the B-chain of ricin E with those of ricin D and of Ricinus communis agglutinin, it was found that the B-chain of ricin E was composed of the N-terminal half of ricin D and C-terminal half R. communis agglutinin. This result suggested that the gene recombination probably occurred at the center region of two B-chain genes of ricin D and R. communis agglutinin.
D000595:Amino Acid Sequence; D002367:Ricinus communis / Q000032:analysis / Q000235:genetics*; D005796:Genes; D037102:Lectins / Q000235:genetics; D010446:Peptide Fragments / Q000032:analysis; D037121:Plant Lectins; D010947:Plants, Toxic; D011995:Recombination, Genetic; D012276:Ricin / Q000235:genetics*; D012278:Ricinus / Q000235:genetics*; D012995:Solubility
D037102:Lectins; D010446:Peptide Fragments; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D012276:Ricin