Preparative liquid chromatography in the field of X-ray contrast agents.
The use of preparative liquid chromatography (LC) for the effective purification of potential X-ray contrast agents or intermediates is described. Pure products in grams to kilogram quantities have been obtained by reversed-phase preparative LC of highly water-soluble non-ionic compounds, a weak aliphatic carboxylic acid and an isophthalic acid derivative. The mobile phases used were mixtures of methanol-water alone or with the addition of ammonium acetate buffers, tetrabutylammonium chloride and phosphate buffer.
D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003287:Contrast Media / Q000032:analysis*; D007472:Iohexol / Q000032:analysis; D008793:Metrizamide / Q000032:analysis; D008794:Metrizoic Acid / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D003287:Contrast Media; D007472:Iohexol; D008794:Metrizoic Acid; D008793:Metrizamide