title: Mitochondria catalyze the reduction of NAD by reduced methylviologen.
abstract: Mitochondria from beef heart and yeast catalyze the reduction of NAD to NADH at the expense of reduced methylviologen (MV+). Based on protein the specific activity of mitochondria for this reaction is about 10 20-times higher than the consumption of oxygen in the presence of succinate or NADH. In 2H2O buffer (4S)-[4-2H]NADH is formed in high enantiomeric excess if the reduced methylviologen is electrochemically regenerated.
mesh_terms: D000818:Animals; D002175:Candida / Q000378:metabolism; D002417:Cattle; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010269:Paraquat / Q000378:metabolism*
chemical_list: D009243:NAD; D010269:Paraquat