title: The metabolism and transport of bovine serum SIgA.
abstract: Experiments were undertaken in six Holstein-Friesian cattle to determine whether secretory IgA (SIgA) could be transported from serum into exocrine body fluids. Preliminary data indicated that when IgG1, IgG2, IgM and SIgA were administered i.v., only SIgA and IgG1 appeared in bile 90 min later at concentrations equal to or exceeding those in serum at the same time. Two hours post-injection, 70% of the SIgA recovered in bile was intact however only 30% co-precipitable with anti-secretory component (SC) while greater than 90% of the administered IgA was precipitated by this method. All recovered IgG1 was of low molecular weight. More detailed studies indicated that the IgA recovered in bile 7 h post-injection or in milk 3 h post-injection, was predominantly lower molecular weight than intact SIgA. Most of this low molecular weight radioactivity was TCA precipitable and ca 50% was dialyzable; these data indicate that TCA-precipitability is an inadequate criterion for determining whether intact SIgA is transported. The radioactivity recovered in parotid saliva was almost entirely non-TCA precipitable and dialyzable. Almost all SIgA recovered in bovine serum remained intact and had a t1/2 of 15.7 h. When transport into milk and bile was calculated from total, recovered radioactivity (i.e. 29% and 2.7, respectively), data compared favorably with those conducted in sheep in which dimeric IgA (without SC) was administered i.v. When we calculated transport on the basis of recovered intact IgA, only 1.47 and 0.54% of the injected dose had been transported into milk and bile, respectively, 24 h later. Most IgA in ruminant bile may be of serum origin although the same appears to be unlikely for the IgA in milk.
mesh_terms: D000818:Animals; D001646:Bile / Q000276:immunology*; D001692:Biological Transport; D002417:Cattle / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000032:analysis / Q000378:metabolism*; D008892:Milk / Q000276:immunology*; D012463:Saliva / Q000276:immunology*
chemical_list: D007071:Immunoglobulin A, Secretory