Quantitative study of cell interaction with collagen and fibronectin.
The interaction of BHK-fibroblasts with collagen or fibronectin-collagen complex was investigated quantitatively. For that purpose an improved method for production of defined cell substrata was developed. The method permitted reproducible coupling of different ligands to glass via an amino or carboxyl group. BHK-cells grown on collagen required a minimum density of 15-20 ng collagen/cm2 for spreading. When grown on fibronectin adsorbed on collagen the cells were found to remove fibronectin from the substratum at a rate of 0.15 pg/(cell X h).
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D006224:Cricetinae; D046508:Culture Techniques / Q000379:methods; D005353:Fibronectins / Q000378:metabolism*; D007668:Kidney; D007700:Kinetics; D012710:Serum Albumin, Bovine / Q000378:metabolism
D005353:Fibronectins; D012710:Serum Albumin, Bovine; D003094:Collagen