The metabolism of deoxyguanosine in mitochondria: a characterization of the phosphorylation process which occurs in intact mitochondria.
Isolated, intact mitochondria were evaluated for their ability to phosphorylate deoxyguanosine. This activity was stimulated by exogenous ATP, substrates for oxidative phosphorylation or added inorganic phosphate. Inhibitors of oxidative phosphorylation lowered the levels of deoxyguanosine phosphorylation. From a Hanes plot, an apparent Km of 0.83 microM deoxyguanosine was calculated for the phosphorylation activity in intact mitochondria. In the presence of a 20-fold excess of added deoxynucleosides, none of those tested were strongly inhibitory. However, added UDP and dTDP were stimulatory and dGTP and dGDP were inhibitory to the phosphorylation of deoxyguanosine. These data show that mitochondria phosphorylate deoxyguanosine and that the process is regulated by other events which take place within the organelle.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D003849:Deoxyguanosine / Q000378:metabolism*; D004579:Electron Transport; D007700:Kinetics; D008293:Malates / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009711:Nucleotides / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology; D010766:Phosphorylation; D051381:Rats; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid
D008293:Malates; D009711:Nucleotides; D010710:Phosphates; D013386:Succinates; D000244:Adenosine Diphosphate; C030298:malic acid; D000255:Adenosine Triphosphate; D019802:Succinic Acid; D003849:Deoxyguanosine