title: Immunoaffinity purification of subcellular particles and organelles.
abstract: The application of immunoaffinity techniques to subcellular fractionation is reviewed and the basic principles underlying the various methods that have been successfully employed, identified. The requirement for organelle-specific antigens and high-avidity antibodies is discussed, as is the widespread use of indirect immunoadsorbents. Approaches for the optimization of immunoaffinity-based subcellular fractionation are suggested.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens; D002458:Cell Fractionation / Q000379:methods; D006801:Humans; D007106:Immune Sera; D007202:Indicators and Reagents; D009940:Organoids / Q000648:ultrastructure*; D013347:Subcellular Fractions / Q000648:ultrastructure*
chemical_list: D000936:Antigen-Antibody Complex; D000941:Antigens; D007106:Immune Sera; D007202:Indicators and Reagents