Malate dehydrogenase species in the cytosolic fraction of chicken liver.
The malate dehydrogenase activity in the cytosolic fraction isolated from chicken hepatocytes is resolved by DEAE-Sephacel chromatography in three active, electrophoretically distinct, species obtained in homogeneous form by affinity chromatography on 5'-AMP-Sepharose and Blue-Sepharose. Two of those species, according to the results obtained, might represent different conformational isomers of the enzyme molecule. Their purified preparations show identical amino-acid compositions and physico-chemical properties very similar to those of the cytosolic isoenzyme of other sources. The third one corresponds to a slight contamination of the mitochondrial isoenzyme.
D000818:Animals; D002645:Chickens; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000201:enzymology; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology
D007527:Isoenzymes; D008291:Malate Dehydrogenase