Non-cross-reactivity of antibodies to murine LDH-C4 with LDH-A4 and LDH-B4.
The induction of infertility by immunization with the sperm-specific lactate dehydrogenase, LDH-C4, suggests its use in a contraceptive vaccine. Development of an immunological contraceptive for human use, however, requires that there be no cross-reactions with somatic tissues. We have demonstrated, using enzyme-linked immunoabsorbence, solid-phase radioimmunoassay, and competitive inhibition radioimmunoassay, that antisera to LDH-C4 is specific and does not cross-react with the somatic isozymes, LDH-A4 and LDH-B4.
D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D008297:Male; D051379:Mice; D011863:Radioimmunoassay; D013094:Spermatozoa / Q000201:enzymology / Q000276:immunology; D013737:Testis / Q000201:enzymology / Q000276:immunology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase