title: Prematurely condensed human sperm chromosomes after in vitro fertilization (IVF).
abstract: During an in vitro fertilization (IVF) program 122 inseminated eggs showing polar body extrusion, but neither formation of pronuclei nor cell cleavage were analysed cytogenetically. Nine of these eggs showed prematurely condensed sperm chromosomes of the G1-phase (G1-PCC) besides the haploid set of maternal metaphase II chromosomes. This phenomenon can be explained by the permanent arrest of the oocytes at metaphase II after sperm penetration and hence the continuing presence of cytoplasmic chromosome condensing factors which lead to the induction of PCC in the sperm nucleus. The overall frequency of this aberrant type of fertilization was calculated to be in the order of 3-4% of all in vitro fertilized eggs.
mesh_terms: D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007621:Karyotyping; D008297:Male; D009865:Oocytes / Q000254:growth & development; D013094:Spermatozoa / Q000648:ultrastructure*