title: Hexose transport in plasma membrane vesicles prepared from L6 rat myoblasts.
abstract: Hexose transport in plasma membrane vesicles prepared from L6 rat myoblasts was shown to be stereospecific, activated by glucose starvation and occurred by both high and low affinity systems. Transport by the high affinity system was shown to occur by an active transport process. Furthermore, the high affinity system was shown to be defective in vesicles prepared from F72 cells (hexose transport mutant). These results indicate that the high affinity hexose transport system is retained in the plasma membrane vesicles. Thus plasma membrane vesicles could be of value in further characterization of the L6 high affinity hexose transport system, without interference from the various metabolic events occurring in whole cells.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism*; D002474:Cell-Free System; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism*; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D009132:Muscles / Q000378:metabolism*; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D006601:Hexoses; D007476:Ionophores; D014475:Uncoupling Agents; D003847:Deoxyglucose; D005947:Glucose