Toxicity of organotin compounds for polymorphonuclear leukocytes: the effect on phagocytosis and exocytosis.
Phagocytosis and concomitant release of enzymes by rabbit polymorphonuclear leukocytes (PMNs) are inhibited by micromolar concentrations of triphenyltin and tributyltin; inhibition by triethyltin occurs at higher concentrations. Chemotactic peptide-induced exocytosis is inhibited at the same concentrations as phagocytosis. Tributyltin causes cell lysis at slightly higher concentrations as required for inhibition of phagocytosis and exocytosis. The organotin compounds have little effect on ATP level in PMNs, which makes an effect on metabolic energy providing processes unlikely. The increase of Ca2+-permeability of the plasma membrane, induced by chemotactic peptide, is inhibited by the organotin compounds. Inhibition of exocytosis by triphenyltin can be counteracted by a number of sulfhydryl compounds. The results suggest that the organotin compounds interfere with PMN function in an early phase of cell activation, where all functions have a common pathway, and where vulnerable sulfhydryl groups play a pivotal role.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects*; D009947:Organotin Compounds / Q000633:toxicity*; D010587:Phagocytosis / Q000187:drug effects*; D011817:Rabbits; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014267:Triethyltin Compounds / Q000633:toxicity*; D015054:Zymosan / Q000378:metabolism
D009947:Organotin Compounds; D013439:Sulfhydryl Reagents; D014220:Trialkyltin Compounds; D014267:Triethyltin Compounds; C011559:tributyltin; C031575:triethyltin; D000255:Adenosine Triphosphate; D015054:Zymosan; C030665:triphenyltin; D002118:Calcium