Isolation of phosvitin: retention of small molecular weight species and staining characteristics on electrophoretic gels.
Phosvitin was prepared from chicken yolk by a variety of published methods, including a new procedure modified from our own original method. Yolk granules and all phosvitin preparations were found to contain a cluster of phosphoproteins ranging in size from Mr 28,000 to 43,000 when electrophoresed on polyacrylamide gradient gels and stained with Stains-all. However, only the current preparation contained three additional phosphoproteins (Mr 13,000, 15,000, and 18,000) that are also present in yolk granules. None of these components could be demonstrated with Coomassie blue.
D000818:Animals; D002232:Carbocyanines; D002645:Chickens; D004527:Egg Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010063:Ovum / Q000032:analysis; D010774:Phosvitin / Q000302:isolation & purification*
D002232:Carbocyanines; D004527:Egg Proteins; C012040:stains-all; D010774:Phosvitin