Resolution and purification of free primase activity from the DNA primase-polymerase alpha complex of HeLa cells.
DNA primase activity has been resolved from a purified DNA primase-polymerase alpha complex of HeLa cells by hydrophobic affinity chromatography on phenylSepharose followed by chromatography on hexylagarose. This procedure provides a good yield (55%) of DNA primase that is free from polymerase alpha. The free DNA primase activity was purified to near homogeneity and its properties characterized. Sodium dodecyl sulfate polyacrylamide gel electrophoretic analysis of the purified free DNA primase showed a major protein staining band of Mr 70,000. The native enzyme in velocity sedimentation has an S20'W of 5. DNA primase synthesizes RNA oligomers with single-stranded M-13 DNA, poly(dT) and poly(dC) templates that are elongated by the DNA polymerase alpha in a manner that has already been described for several purified eukaryotic DNA primase-polymerase alpha complexes. The purified free DNA primase activity is resistant to neutralizing anti-human DNA polymerase alpha antibodies, BuPdGTP and aphidicolin that specifically inhibit the free DNA polymerase alpha and also DNA polymerase alpha complexed with the primase. The free primase activity is more sensitive to monovalent salt concentrations and is more labile than polymerase alpha. Taken together these results indicate that the DNA primase and polymerase alpha activities of the DNA primase-polymerase alpha complex reside on separate polypeptides that associate tightly through hydrophobic interactions.
D004257:DNA Polymerase II / Q000302:isolation & purification* / Q000378:metabolism; D019915:DNA Primase; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D013698:Templates, Genetic
D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004257:DNA Polymerase II