Loss of quin 2 accompanies degranulation of mast cells.
Stimulation of quin 2 loaded mast cells in the presence of 1 mM extracellular calcium produced a rapid and sustained increase in quin 2 fluorescence. This was accompanied by degranulation and the release of histamine. When Ca was replaced by EGTA or when Mn was present, a decrease in fluorescence accompanied degranulation. The increase in quin 2 fluorescence accompanying stimulation of mast cells appears to be due to the interaction of extracellular Ca with quin 2 associated with the secretory granule matrix released upon exocytosis.
D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D008297:Male; D008345:Manganese / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000634:Aminoquinolines; D005456:Fluorescent Dyes; D003189:p-Methoxy-N-methylphenethylamine; D008345:Manganese; C035739:Quin2; D002118:Calcium