title: [Thermal sensitivity of Ehrlich ascites cancer cells forming colonies in agar cultures in diffusion chambers].
abstract: Thermosensitivity of clonogenic ascites Ehrlich carcinoma cells (AEC), forming colonies in agar cultures in diffusion chambers was studied when heating them in vitro at 41-44 degrees C. The clonogenic cells of AEC are shown not to essentially differ both in the colony-forming efficiency (CFE) and in the proliferative status on the 3rd and 7th days. No essential differences are revealed in thermosensitivity of such cells. The heating time-survival curves are exponential or S-shaped with a small "shoulder". The temperature increase by 1 degree C resulted in a 2-2.5-fold decrease in the time of effective hyperthermia treatment. The values of D0 for cells treated with 42 degrees C in different experiments varied from 20.1 min to 24.6 min. Only one type of thermal damage reaction with the energy of inactivation about 150 kcal/M was observed in AEC cells in the temperature range studied.
mesh_terms: D000362:Agar; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000503:physiopathology*; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006358:Hot Temperature; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
chemical_list: D004273:DNA, Neoplasm; D000362:Agar
vernacular_title: Termochuvstvitel'nost' kletok astsitnogo raka Erlikha, formiruiushchikh kolonii v agarovykh kul'turakh v diffuzionnykh kamerakh.