[Determination of protein binding of drugs using equilibrium dialysis. Influence of volume displacement caused by osmotic pressure].
By the determination of the free concentration of a drug using the equilibrium dialysis method, a volume shift could be observed, inducing a dilution of the protein medium. The value of this volume shift varies with the plasmatic sample and could produce a misdetermination of the free fraction which could be important, essentially for the drugs strongly bound to proteins.
D001798:Blood Proteins / Q000378:metabolism*; D003956:Dialysis / Q000379:methods; D008954:Models, Biological; D009997:Osmotic Pressure; D004364:Pharmaceutical Preparations / Q000097:blood*; D011485:Protein Binding
D001798:Blood Proteins; D004364:Pharmaceutical Preparations
Détermination de la fixation protéique des médicaments par dialyse à l'équilibre. Influence du déplacement de volume sous l'effet de la pression osmotique.