Lysosomal enzyme activities in fresh and frozen chorionic villi and in cultured trophoblasts.
Fifteen lysosomal enzyme activities were compared in 14 presumed normal chorionic villus specimens that were each divided, processed and analyzed as fresh tissue, tissue frozen for 1 week, and cultures established from minced whole villi. Most of the activities determined in the chorionic villus tissue were not affected significantly by freezing. However, activities for most enzymes were significantly different from those determined in the cultured cells. Our experience with first trimester prenatal evaluations for several lysosomal disorders showed that the limited amount of tissue obtained is not always sufficient for thorough analysis and thus, cultured trophoblasts derived from the tissue specimen should also be examined. The results of this study stress the importance of using appropriate tissue-type and cell-type controls to establish the normal range in the respective analyses.
D001192:Arylsulfatases / Q000032:analysis; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000201:enzymology*; D005260:Female; D005615:Freezing; D005696:Galactosidases / Q000032:analysis; D006596:Hexosaminidases / Q000032:analysis; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D011247:Pregnancy; D014327:Trophoblasts / Q000201:enzymology*
D001192:Arylsulfatases; D005696:Galactosidases; D006596:Hexosaminidases