[A simplified variant of the Maxam-Gilbert method for determining the primary structure of oligonucleotides and DNA fragments].
A modification to the Maxam-Gilbert method is proposed that involves precipitation of the nucleotide material with the acetone solution of lithium perchlorate after the completion of chemical reactions to remove the reagents. Modification of cytosine residues is carried out in the presence of lithium chloride. The new mode of precipitation simplifies and speeds up the analysis of oligonucleotides and DNA fragments.
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D009841:Oligonucleotides / Q000032:analysis*; D010957:Plasmids
D009841:Oligonucleotides; D004247:DNA
Uproshchennyĭ variant metoda Maksama-Gilberta dlia opredeleniia pervichnoĭ struktury oligonukleotidov i fragmentov DNK.