title: Induction of cytochrome P-450-dependent sulfonylurea metabolism in Streptomyces griseolus.
abstract: Inducible cometabolism of several sulfonylurea herbicides by Streptomyces griseolus has been shown to occur by hydroxylation, O-dealkylation, or deesterification reactions. Only after growth of the bacterium in the presence of sulfonylurea did cell-free extracts exhibit NAD(P)H-dependent sulfonylurea metabolism. These extracts were shown to contain elevated levels of soluble cytochrome P-450 and exhibit sulfonylurea induced difference spectra consistent with binding of substrate to cytochrome(s) P-450. These results establish the presence of an inducible cytochrome P-450-dependent sulfonylurea metabolizing system in S. griseolus.
mesh_terms: D002701:Chloramphenicol / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D006540:Herbicides / Q000378:metabolism*; D012988:Soil Microbiology; D013302:Streptomyces / Q000201:enzymology*; D013453:Sulfonylurea Compounds / Q000378:metabolism*
chemical_list: D006540:Herbicides; D013453:Sulfonylurea Compounds; D002701:Chloramphenicol; D003577:Cytochrome P-450 Enzyme System; C042031:sulfometuron methyl