title: Presence of estrogen receptors in the hen hypothalamus and pituitary.
abstract: In the hen, the preoptic area, medial basal hypothalamus, and anterior lobe of the pituitary showed a greater uptake of [3H]estradiol-17 beta (E2) in vivo than other tissues. This uptake was decreased when unlabeled diethylstilbestrol was injected together with the [3H]E2 or when unlabeled E2 was injected before the [3H]E2 injection. A specific estrogen binding component having properties of a receptor was found in vitro in both soluble and insoluble fractions of these tissues in a hypotonic buffer solution. The administration of E2 in vivo caused a marked decrease in the estrogen receptor binding in the soluble fraction with a concomitant increase in binding in the insoluble fraction; as a result, total binding (sum of the bindings in soluble and insoluble fraction) did not change.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002645:Chickens; D004054:Diethylstilbestrol / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D007700:Kinetics; D009928:Organ Specificity; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011960:Receptors, Estrogen; D004958:Estradiol; D004054:Diethylstilbestrol