title: The successful use of human amniotic fluid for mouse embryo culture and human in vitro fertilization, embryo culture, and transfer.
abstract: The development of mouse and human embryos was assessed in human amniotic fluid to determine its suitability as a culture medium for human in vitro fertilization (IVF). Two-cell mouse embryos developed to blastocysts after 72 hours at rates similar to that in Whittingham's T6 + 10% fetal calf serum. Significantly more mouse embryos hatched in amniotic fluid. No difference was found between individual patient's amniotic fluids obtained at 16 to 21 weeks' gestation. A preliminary trial comparing amniotic fluid with T6 + maternal serum in human IVF showed no significant difference in fertilization rate and embryo development during 42 to 48 hours in vitro. Expanded blastocysts were obtained in amniotic fluid after 5 days in vitro. Four pregnancies were obtained in 9 patients' transferred embryos grown in amniotic fluid and with 2 or 12 patients' transferred embryos grown in T6 + maternal serum.
mesh_terms: D000653:Amniotic Fluid; D000818:Animals; D003470:Culture Media; D046508:Culture Techniques; D004624:Embryo Transfer / Q000379:methods*; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D051379:Mice
chemical_list: D003470:Culture Media