Physiologic aging of mature porcine erythrocytes: effects of various metabolites, antimetabolites, and physical stressors.
Red blood cells were collected from Landrace X Duroc pigs in pooled and single batches. The RBC were stored for 24 hours to 20 days and exposed to 1 or more chemical and physical stressors. The chemicals were pyruvate, lactate, inosine, concanavalin A-luminol-bovine serum albumin conjugate, hydrogen peroxide, L-mimosine, and 3-amino-L-tyrosine. Physical stressors included thermogenic microwave radiation (2,450 MHz, mean specific absorption rate of 91 W/kg) and conventional heating with hot air or hot-water bath. Heating erythrocytes to 43 C for 10 minutes with microwaves or hot air did not significantly (P greater than 0.05) increase hemolysis, compared with hemolysis of RBC at 4 C (controls). Pyruvate or lactate did not affect the RBC under these conditions. Hemolysis of cells coated with concanavalin A-luminol-bovine serum albumin and heated to 43 C for 10 minutes by use of microwaves or hot air was significantly (P less than 0.05 by Student's t test) greater (48%) than that of RBC at 4 C (controls). The method of heating or the presence of pyruvate, lactate, or inosine did not have a significant (P greater than 0.05) effect on hemolysis. Lysis of RBC (14 days after collection) coated with concanavalin A-luminol-bovine serum albumin and stored at 4 C was not significantly different (P greater than 0.05) than that of noncoated cells (6 days after collection) stored at 4 C (control). When RBC were heated 20 days after collection to 48 C for 30 minutes, using a hot water bath, hemolysis was 60.6% greater than that of control cells (4 C).(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D004905:Erythrocyte Aging / Q000187:drug effects; D004912:Erythrocytes / Q000378:metabolism; D006461:Hemolysis; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007288:Inosine / Q000494:pharmacology; D007773:Lactates / Q000494:pharmacology; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008898:Mimosine / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013552:Swine / Q000097:blood*; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives / Q000494:pharmacology
D007773:Lactates; D011773:Pyruvates; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; C019253:3-aminotyrosine; D014443:Tyrosine; D008898:Mimosine; D007288:Inosine; D008165:Luminol; D006861:Hydrogen Peroxide