Complement activation in synovial fluid and tissue from patients with juvenile rheumatoid arthritis.
Synovial fluid (SF) and synovial tissue from 10 patients with juvenile rheumatoid arthritis were examined. The SFs were heterogeneous with respect to the degree of complement activation. Quantification of C3dg and the terminal complement complex revealed a positive correlation between activation of the early and the late parts of the cascade in all patients. The amount of C-reactive protein and the number of white blood cells in the SF correlated significantly with the degree of complement activation. Weak deposits of C3, C3dg, or terminal complement complex were observed in a few vessels in the synovial tissue from 5 of the patients. There was no correlation between complement activity in SF and in the corresponding tissue. Furthermore, there was no correlation between clinical activity in the joints and the degree of complement activation. It is concluded that there is a discrepancy between synovial tissue and synovial fluid with respect to complement activation. C-reactive protein may, to some extent, be responsible for activation in SF, and the accumulation of white blood cells may be due to complement activation products.
D000293:Adolescent; D001171:Arthritis, Juvenile / Q000276:immunology*; D002097:C-Reactive Protein / Q000032:analysis; D002648:Child; D003167:Complement Activation; D003176:Complement C3 / Q000032:analysis; D003179:Complement C3b / Q000032:analysis; D015938:Complement Membrane Attack Complex; D003165:Complement System Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D009077:Mucins; D010446:Peptide Fragments / Q000032:analysis; D013582:Synovial Fluid / Q000276:immunology*; D013583:Synovial Membrane / Q000276:immunology*
D003176:Complement C3; D015938:Complement Membrane Attack Complex; D009077:Mucins; D010446:Peptide Fragments; C045477:complement C3d,g; D003179:Complement C3b; D003165:Complement System Proteins; D002097:C-Reactive Protein