Uranyl action on sugar transport across rat jejunum.
The effect of uranyl on sugar transport across rat jejunum has been studied in vitro and in vivo. D-glucose and D-galactose accumulation in jejunum rings at pH 6.0 is inhibited about 40-65% by 1 mM uranyl nitrate. This inhibition is lower than that produced by 0.5 mM phlorizin. The effect was very similar when the incubation of the rings with the sugar was made in the absence of uranyl, after preincubation with the inhibitor. Washing with 10 mM EDTA reverted uranyl inhibition only slightly. D-fructose entry was weakly inhibited by uranyl. Glucose absorption in vivo along perfusion periods of 1 min was not affected by the presence of uranyl (0.001 to 1 mM) in the sugar solution, but the exposure of the mucosa to 0.1 mM uranyl at pH 6.5 for 10 min inhibited sugar absorption at the same pH in the subsequent periods of perfusion. Results suggest that uranyl impairs sugar transport by binding to protein chemical groups at the surface or in deeper sites of enterocyte membranes, a process that requires some minutes to be accomplished.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D006601:Hexoses / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014501:Uranium / Q000494:pharmacology*; D014502:Uranyl Nitrate / Q000494:pharmacology*
D006601:Hexoses; D014502:Uranyl Nitrate; D014501:Uranium