FTIR-ATR studies of the effect of shear rate upon albumin adsorption onto polyurethaneurea.
The effect of wall shear rate upon albumin adsorption onto a polyurethaneurea, Biomer, was studied using FTIR-ATR spectroscopy. In these experiments, nondiffusion controlled adsorption was observed from 10 mg/ml solutions at known wall shear rates. Results show that increasing wall shear rate during adsorption does not significantly affect the adsorption kinetics, but does decrease the rate of protein desorption. These results suggest that albumin adsorbed at higher shear rates is more tightly bound to the surface. Comparison of the data to simple mathematical models suggests that more than one layer of protein adsorbs in 2 h.
D000327:Adsorption; D001672:Biocompatible Materials; D007700:Kinetics; D008956:Models, Chemical; D011140:Polyurethanes; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D013055:Spectrophotometry, Infrared; D013499:Surface Properties
D001672:Biocompatible Materials; D011140:Polyurethanes; D012710:Serum Albumin, Bovine; C031892:biomer