title: A microcomputer system for video image analysis and diagnostic microdensitometry.
abstract: A system for microdensitometry based on a microcomputer, video digitizer and solid-state camera has been developed. Image analysis and densitometry are achieved with convenient control over image editing and calibration. The linear photometric properties of the imaging device enable measurements of high accuracy. The system has proven to give rapid and repeatable performance for determining DNA content distribution from measurements of Feulgen-stained cell nuclei. The results show that a practical image analysis microdensitometer can be designed using a readily available microcomputer. The low cost and simple operation are of benefit for diagnostic applications in which flow cytometry is not possible, the time required for microscope photometry is too great or an automated image analyzer and support staff are not available.
mesh_terms: D000818:Animals; D003196:Computer Graphics; D003201:Computers; D004247:DNA / Q000032:analysis*; D003720:Densitometry / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008099:Liver / Q000648:ultrastructure; D008214:Lymphocytes / Q000648:ultrastructure; D008838:Microcomputers; D051381:Rats; D012984:Software; D014741:Video Recording
chemical_list: D004247:DNA