Detection of desamido forms of purified bovine growth hormone.
Tryptic peptide mapping and amino acid sequencing were used for the identification of desamido forms of bovine growth hormone. The major desamido forms of bovine growth hormone resulted from deamidation of asparagine in position 13, 148 and glutamine 140. These forms did not seem to be produced by prolonged treatment of bovine growth hormone in tryptic hydrolysis.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D013006:Growth Hormone / Q000032:analysis*; D006868:Hydrolysis; D010449:Peptide Mapping; D011487:Protein Conformation; D014357:Trypsin
D013006:Growth Hormone; D014357:Trypsin