[Isolation of membrane antigens of mammary gland epithelial cells from membranes of human milk fat globules].
Seven antigens, belonging to various structures of bilayer: ArI (lactoferrin) and Ar5 (alpha-lactalbumin) - adsorbed proteins; Ar2 and Ar14 - peripheric components; Ar3, Ar4 and Ar13 - integral structures, were found after treatment of fatty globule membranes from human milk using the agents with improved extracting activity (isotonic solution, 1 M MgCl2, 1% Triton X-100, combined effect of 1% Triton X-100 and sonication). The procedure developed enabled to separate these antigens and to isolate them from the main milk secretory proteins lactoferrin and alpha-lactalbumin. Towards highly purified preparations of the membrane antigens 3 and 4 monospecific antisera were raised, which allowed to identify these components among tissue-specific antigens of mammary gland capable to be maintained in malignization of the tissue. Ar4 was also found in tumoral tissues of ovary, lung and stomach.
D000818:Animals; D000954:Antigens, Surface / Q000302:isolation & purification*; D002417:Cattle; D006801:Humans; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000302:isolation & purification*; D008892:Milk / Q000276:immunology; D008895:Milk, Human / Q000276:immunology*; D018396:Mucin-1; D012995:Solubility
D000954:Antigens, Surface; D008565:Membrane Proteins; D018396:Mucin-1
Vydelenie membrannykh antigenov épitelial'nykh kletok molochnoĭ zhelezy iz membran zhirovykh globul zhenskogo moloka.