title: The FRAME multicentre project on in vitro cytotoxicology.
abstract: An in vitro method for assessing the relative acute toxicities of chemicals was developed through the collaboration of four tissue culture laboratories. Cytotoxicity was measured by using a dye-binding method to determine inhibition of the proliferation of BCL-D1 cells 72 hr after addition of the test chemical. A blind trial with 50 chemicals showed that the test procedure gave results with a high degree of interlaboratory reproducibility.
mesh_terms: D001711:Biotransformation; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D012995:Solubility; D014116:Toxicology / Q000379:methods*