Gene distribution and nucleotide sequence organization in the mouse genome.
Mouse DNA was fractionated by preparative centrifugation in density gradients of Cs2SO4 containing 3,6-bis(acetatomercurimethyl)dioxane (BAMD). The effects of temperature, BAMD/nucleotide molar ratio and solvent on the fractionation were explored. The fractions so obtained were investigated by analytical centrifugation in CsCl density gradient and by hybridization with a number of gene probes. These approaches led to the definition of satisfactory conditions for the rapid fractionation of mouse DNA; to the localization of a number of genes in mouse DNA fractions; and to a better understanding of the mosaic organization of the mouse genome and, more specifically, to a better estimate of both the intermolecular and intramolecular compositional heterogeneity of mouse DNA in the (75-150) X 10(3)-base size range.
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002499:Centrifugation, Density Gradient / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005796:Genes; D005914:Globins / Q000235:genetics; D008099:Liver / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D009857:Oncogenes
D000199:Actins; D005914:Globins; D004247:DNA