Stimulation of human blood mononuclear cell procoagulant activity by cyclosporin A.
Using a one-stage assay of plasma recalcification time, we have examined the procoagulant activity (PA) of human blood mononuclear cells following incubation for 20 h in the presence of cyclosporin A (CsA; 50-5,000 ng/ml). CsA alone caused a significant, dose-related increase in PA above basal values at 1,000 and 5,000 ng/ml. At these concentrations, it also enhanced the increases in PA produced by concanavalin A, phytohaemagglutinin and bacterial endotoxin. These effects of CsA were totally inhibited by actinomycin D or cycloheximide.
D001779:Blood Coagulation Factors; D003208:Concanavalin A / Q000494:pharmacology; D003524:Cyclosporins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D013268:Stimulation, Chemical
D001779:Blood Coagulation Factors; D003524:Cyclosporins; D008070:Lipopolysaccharides; C045071:leukocyte procoagulant activity; D003208:Concanavalin A