Sequencing and identification of a cDNA clone for tomato polygalacturonase.
The 2a isoenzyme of tomato polygalacturonase was purified from ripe fruit and characterised. The N-terminal amino acid sequence of the protein was determined in order to identify polygalacturonase cDNA clones. The nucleotide sequence of a ripening-related cDNA (pTOM 6) was determined and found to encode the N-terminal sequence of mature polygalacturonase 2a. The complete open reading frame encodes a polypeptide of molecular weight 50,051, including a putative pre-sequence of 71 amino acids.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D005796:Genes; D006026:Glycoside Hydrolases / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011096:Polygalacturonase / Q000235:genetics* / Q000302:isolation & purification
D007527:Isoenzymes; D004247:DNA; D006026:Glycoside Hydrolases; D011096:Polygalacturonase