Glycoproteins of human teratocarcinoma cells (PA1) carry both anomers of O-glycosyl-linked D-galactopyranosyl-(1----3)-2-acetamido- 2-deoxy-alpha-D-galactopyranosyl group.
Two disaccharide alcohols, alpha-D-Galp(1----3)-GalNAcol and beta-D-Galp-(1----3)-GalNAcol, together with a GalNAcol-containing tetra- or penta-saccharide alcohol, were released from human embryonal carcinoma cells of line PA1 by reductive beta-elimination. The disaccharides were identified by exoglycosidase digestions and by periodate oxidation. The results were confirmed by affinity chromatography of the disaccharide alcohols on immobilized Bandeirea simplicifolia lectin and by chromatography of the parent glycopeptides on immobilized peanut lectin.
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D005696:Galactosidases; D006020:Glycopeptides / Q000302:isolation & purification; D006023:Glycoproteins; D006801:Humans; D007536:Isomerism; D009844:Oligosaccharides / Q000302:isolation & purification; D013724:Teratoma / Q000032:analysis*
D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D005696:Galactosidases