PAF-acether (1-O-hexadecyl/octadecyl-2-acetyl-sn-glycero-3-phosphocholine)-induced fibrinogen binding to platelets depends on metabolic energy.
A combination of CN- and 2-deoxy-D-glucose decreases the binding of fibrinogen to platelets stimulated with PAF-acether (1-O-hexadecyl/octadecyl-2-acetyl-sn-glycero-3-phosphocholine). Decreased binding is found after pretreatment with metabolic inhibitors, thereby lowering the energy content before stimulation as well as at various stages after stimulation of undisturbed cells. Binding and ATP hydrolysis occur in parallel, suggesting tight coupling between both phenomena. Energy appears to be predominantly required for exposure and maintenance of accessible binding sites, whereas the interaction between fibrinogen and the exposed sites does not depend on metabolic energy.
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D011485:Protein Binding / Q000187:drug effects; D013268:Stimulation, Chemical
D003486:Cyanides; D010972:Platelet Activating Factor; C026613:1-0-octadecyl 2-0-acetyl sn-glycero-3-phosphorylcholine; D005340:Fibrinogen; D003847:Deoxyglucose