title: Reduction in potency and reversal of left-shifting activity of BW12C with the major and minor components of chicken hemoglobin.
abstract: The effects of the left-shifting, anti-sickling compound BW12C (5-(2-formyl-3-hydroxyphenoxy)pentanoic acid) on the oxygen saturation curve of whole chicken blood and the isolated major (AII) and minor (AI) components of chicken hemoglobin have been studied. The results support the postulated major binding mode for BW12C to human hemoglobin of bridging between the alpha-chain terminal amino groups in the oxy conformation with an important hydrophobic component contributed mainly by Pro 77 alpha residues. In chicken AII (Pro 77 alpha----Ser) BW12C still left-shifts at high concentrations but its potency is greatly reduced (at least 10-fold). In chicken AI (Pro 77 alpha----Ser and Val 1 alpha----Met) BW12C is a right-shifter with a potency comparable to that of 2,3-diphosphoglycerate suggesting that binding at the beta-chain termini in the deoxy conformation is now dominant with alpha-chain binding no longer significant.
mesh_terms: D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D001547:Benzaldehydes; D002645:Chickens; D006454:Hemoglobins / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D010108:Oxyhemoglobins / Q000187:drug effects / Q000378:metabolism
chemical_list: D000447:Aldehydes; D001547:Benzaldehydes; D006454:Hemoglobins; D010108:Oxyhemoglobins; C039599:5-(2-formyl-3-hydroxyphenoxy)pentanoic acid