A sandwich assay to detect and characterize syngeneic anti-idiotypic antibodies to murine anti-HLA and tumor associated antigen monoclonal antibodies.
The parameters of a sandwich assay to analyze syngeneic polyclonal and monoclonal anti-idiotypic antibodies to murine anti-HLA and anti-human melanoma associated antigen monoclonal antibodies have been characterized. Furthermore, the sensitivity of the sandwich assay has been compared with that of assays widely used to characterize anti-idiotypic antibodies, i.e., the binding assay to F(ab')2 fragments of monoclonal antibodies and the radioimmunoassay. The latter is more sensitive than the sandwich assay when monoclonal anti-idiotypic antibodies are tested, but less sensitive when anti-idiotypic antisera are analyzed. Both assays are less sensitive than the binding assay to F(ab')2 fragments of monoclonal antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008545:Melanoma / Q000276:immunology; D051379:Mice; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006680:HLA Antigens; D007130:Immunoglobulin Idiotypes