XANES spectroscopy of carp hemoglobin-iron in correlation with the affinity changes of the protein for ligand.
The strong variation of ligand-binding properties with pH for carp hemoglobin is not reflected in the electronic distribution of the heme-iron. Thus, we can suppose that hemoglobin affinity is directly controlled by the protein and by some particular changes of the iron atom.
D000494:Allosteric Regulation; D000818:Animals; D002347:Carps; D006454:Hemoglobins; D006863:Hydrogen-Ion Concentration; D007501:Iron; D008024:Ligands; D013057:Spectrum Analysis / Q000379:methods; D014965:X-Rays
D006454:Hemoglobins; D008024:Ligands; D007501:Iron