title: In vivo covalent binding of aminofluorene derivatives to mitochondrial liver DNA of rats pretreated with phenobarbital.
abstract: The covalent binding index (CBI) of aminofluorene derivatives (AF) to both nuclear (n) and mitochondrial (mt) hepatic DNA of rats 16 h after i.p. injection of N-acetyl-2-aminofluorene (AAF) has been determined. It was 69 and 212 respectively, i.e. about 3 times higher in mtDNA than in the nDNA. When rats were pretreated for 5 days with phenobarbital (PB) the two CBI decreased in parallel indicating a detoxification of AAF preventing its binding to both nDNA and mtDNA.
mesh_terms: D015073:2-Acetylaminofluorene / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004272:DNA, Mitochondrial; D015073:2-Acetylaminofluorene; D010634:Phenobarbital