Suppression by 1,25(OH)2D3 of transcription of the pre-proparathyroid hormone gene.
When bovine parathyroid cells in culture are exposed to the active vitamin D metabolite 1,25(OH)2D3, a significant decrease in the steady-state levels of pre-proparathyroid hormone (pre-proPTH) mRNA occurs. The possibility that the fall in specific mRNA is due to a decrease in rate of transcription of the PTH gene was examined in this study. In the presence of 1,25(OH)2D3, there was a rapid and steady decline in PTH gene transcription rate which fell to a minimum of 10-15% of control at 24 h. The effect was observed at physiological levels (10(-11)M) and was also fully reversible.
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002417:Cattle; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D010280:Parathyroid Glands / Q000502:physiology*; D010281:Parathyroid Hormone / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D010281:Parathyroid Hormone; D011498:Protein Precursors; D012333:RNA, Messenger; C019056:preproparathormone; D002117:Calcitriol