Dipeptide analysis in human urine.
Fractions of dipeptides, obtained from human urine by a combination of cation-exchange chromatography, ligand-exchange chromatography and reversed-phase chromatography, were transformed into their N-heptafluorobutyryl methyl ester derivatives and then subjected to capillary gas chromatography. The profiles obtained indicate the presence of many dipeptides in human urine. For the first time, alpha-Asp-Hyp, Pro-Phe and gamma-Glu-Phe were detected in the urine of healthy individuals.
D002849:Chromatography, Gas; D004151:Dipeptides / Q000652:urine*; D005466:Fluorocarbons; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents
D004151:Dipeptides; D005466:Fluorocarbons; D007202:Indicators and Reagents; C051022:heptafluorobutyryl methyl ester