A simple and rapid method for the purification of ovine pineal arylalkylamine N-acetyltransferase.
A two-step chromatographic procedure has been developed for the purification of ovine pineal arylalkylamine N-acetyltransferase (EC 2.3.1.87), based on the principles of disulfide exchange and anion exchange. The enzyme from 20 ovine pineal glands can be purified about 500-fold in a day; recovery is about 5%. Polyacrylamide gel electrophoretic analysis of the final preparation shows four major bands; one appears to be arylalkylamine N-acetyltransferase.
D000123:Acetyltransferases / Q000302:isolation & purification*; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010870:Pineal Gland / Q000201:enzymology*; D012756:Sheep
D000123:Acetyltransferases; D001191:Arylamine N-Acetyltransferase