Synthesis of milk specific fatty acids and proteins by dispersed goat mammary-gland epithelial cells.
The method now described for preparation of dispersed lactating goat mammary-gland cells gives a high yield of morphologically and functionally normal mammary cells. The cells synthesize specific goat milk fatty acids in the right proportions, and they respond to hormones by increased protein synthesis. The cells can be frozen and thawed without losing the above properties, which makes them an excellent tool for metabolic and hormonal studies.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002469:Cell Separation; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006041:Goats; D007774:Lactation / Q000378:metabolism*; D050356:Lipid Metabolism; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D008854:Microscopy, Electron; D008892:Milk / Q000378:metabolism*; D008894:Milk Proteins / Q000096:biosynthesis*; D011247:Pregnancy
D000085:Acetates; D005227:Fatty Acids; D008894:Milk Proteins; D019342:Acetic Acid