Effects of AF64A on [3H]acetylcholine synthesis in neuron-enriched primary brain cell cultures.
[3H]Acetylcholine (ACh) synthesis was measured in primary neuronal cultures from neonatal rat brains. Neuronal [3H]ACh synthesis was blocked by hemicholinium-3 and depended on the age of the cultures, increasing for ca. 10 days, and eventually declining. The irreversible inhibitor AF64A (10 or 30 microM) inhibited [3H]ACh synthesis from [3H]choline at concentrations (10 or 30 microM) with affecting choline acetyltransferase activity. Nine-day-old cultures recovered 90% of their [3H]ACh synthesis within 7 days after AF64A, while 13-day-old cultures never recovered. These results suggest that the turnover of neuronal choline transporters is age-related.
D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D000831:Animals, Newborn; D001388:Aziridines / Q000494:pharmacology*; D001389:Azirines / Q000494:pharmacology*; D001692:Biological Transport; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D002794:Choline / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D001388:Aziridines; D001389:Azirines; C044894:ethylcholine aziridinium; D002794:Choline; D000109:Acetylcholine