title: Effect of subchronic dietary administration of butylated hydroxyanisole on canine stomach and hepatic tissue.
abstract: Beagle dogs, 29 males and 30 females, were assigned to feeding groups of 0, 1.0 and 1.3% butylated hydroxyanisole (BHA) for 180 days. Animals were observed daily for physical signs of pharmacological or toxicological effect. Except for the production of a reddish-brown urine, no physical signs attributable to BHA administration were observed. Both food consumption and body-weight gain were reduced in the BHA-treated animals. Fifty-one animals completed the study. At termination, tissues were examined for gross BHA-related effects, and specimens were taken for enzyme analysis, light microscopy, electron microscopy and morphometric analysis. The liver showed a significant weight increase over the control in both sexes at both BHA dose levels. Ultrastructural examination of the liver of BHA-treated animals revealed proliferation of smooth endoplasmic reticulum and hepatocytic cytoplasmic myelinoid bodies. Enzyme analysis of hepatic tissue showed a significant increase in mixed-function oxidases, UDP glucuronyl transferase, glutathione S-transferase and epoxide hydratase in the BHA-treated dogs compared with the controls. Light microscopy revealed no proliferative/hyperplastic lesions of the stomach/gastric epithelium. Electron microscopic examination of the lower oesophagus and stomach specimens from representative animals from each treatment group failed to reveal any treatment-related effect as compared with controls.
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D004285:Dogs; D004947:Esophagus / Q000187:drug effects / Q000473:pathology; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D013270:Stomach / Q000187:drug effects* / Q000473:pathology
chemical_list: D002083:Butylated Hydroxyanisole