The effect of 1-beta-D-arabinofuranosyl-cytosine on the expression of the common fragile site at 3p14.
The effect of the G2-treatment of 1-beta-D-arabino-furanosyl-cytosine (araC) on the expression of the common fragile site at 3p14 (FRA3B) was studied. A significantly increased frequency of FRA3B induced by G2 treatment of araC was found in the lymphocytes grown in folate-deficient medium (positive rate 100%). A relatively low frequency of FRA3B was also induced in the cultures with folate in four of the seven subjects. There is a synergistic effect between araC and growth in folate-deficient medium on the induction of FRA3B. The results suggest that the DNA lesions related to the expression of FRA3B induce the long-patch repair and that the low DNA polymerase alpha activity and inefficient repair process during G2 phase is involved in the expression of FRA3B.
D002478:Cells, Cultured; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002893:Chromosomes, Human, Pair 3; D003561:Cytarabine / Q000494:pharmacology*; D004260:DNA Repair; D005260:Female; D005492:Folic Acid / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male
D003561:Cytarabine; D005492:Folic Acid