[Use of mathematical theory in experiments evaluating the protective properties of antimicrobial preparations].
Mathematical design of multifactorial experiments in investigation of protective properties of antimicrobial drugs provided acceleration of preclinical study of new antimicrobial drugs, increasing its level and decreasing its size and cost. The advantage of the experiment mathematical design in investigation and estimation of protective properties of antimicrobial drugs was shown on a model of doxycycline chemotherapy of experimental anthracic infection caused by the vaccinal strain of the causative agent in albino mice. Second order quasi-D-optimal plans for 3-, 4- and 5-factorial experiments are presented and variation ranges of the main factors affecting chemotherapeutic activity of antimicrobial drugs are recommended: daily doses of the antibiotic, the inoculum size, the time of chemotherapy initiation after inoculation and the period of the preventive treatment. The main advantages of the experiment mathematical design in preclinical chemotherapy, along with economic utilization of experimental animals, are the following: possible estimation of the effect of every of the active factors on drug protective properties, objective evaluation of protective activity of drugs under various regimens of their use and feasibility of developing optimal schemes for drug use.
D000818:Animals; D000881:Anthrax / Q000188:drug therapy; D000900:Anti-Bacterial Agents / Q000627:therapeutic use* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004318:Doxycycline / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007928:Lethal Dose 50; D008433:Mathematics; D051379:Mice; D012107:Research Design; D013997:Time Factors
D000900:Anti-Bacterial Agents; D004318:Doxycycline
Primenenie matematicheskoĭ teorii éksperimenta dlia otsenki zashchitnykh svoĭstv protivomikrobnykh preparatov.