An immunocytochemical marker for the complex granules of tick salivary glands which traces e-granule shedding to interstitial labyrinthine spaces.
An antigenic protein (12C), previously isolated from salivary glands of Rhipicephalus appendiculatus, as a possible factor in host resistance to repeated tick infestation, is labeled by protein A-gold immunocytochemistry in adult feeding ticks. It is a component of the gland's complex a-, d- and e-granules and also appears within the chitinous walls of intercalated ducts. In females, between days 4 and 7 of feeding, labeled e-granules appear also within the labyrinthine spaces of acinus type III, apparently released from e-cells as the abluminal interstitial cells initiate formation of a basolateral labyrinth. Granules thus shed are fragmented by interstitial cell processes, some fragments being phagocytized, others disintegrating to single point label scattered throughout the labyrinth. The latter possibly may pass into the acinar lumen. By the eighth day the label is gone from the labyrinth.
D000818:Animals; D000906:Antibodies; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D007118:Immunoassay; D008854:Microscopy, Electron; D012469:Salivary Glands / Q000648:ultrastructure*; D013987:Ticks / Q000166:cytology*
D000906:Antibodies