A rapid HPLC method for determination of adenylate energy charge.
A simple procedure is described for separation and analysis of adenine nucleotides in tissue extracts, utilizing anion exchange HPLC. Determination of AMP, ADP, and ATP takes 10 min per sample.
D000244:Adenosine Diphosphate / Q000302:isolation & purification*; D000249:Adenosine Monophosphate / Q000302:isolation & purification*; D000255:Adenosine Triphosphate / Q000302:isolation & purification*; D000818:Animals; D049872:Bivalvia; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004734:Energy Metabolism; D008974:Mollusca
D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate