Studies on the antitumor lectins isolated from the seeds of Ricinus communis (castor bean).
Three toxic proteins and one agglutinin were purified from the seeds of Ricinus communis by a simple and fast method using Sepharose 4-B affinity chromatography followed by Sephadex G-100 gel filtration. The weakly adsorbed ricins A and B were retarded and eluted with the buffer from the affinity chromatographic column, while ricin C and ricinus agglutinin had to be eluted with 0.1 M galactose. The molecular weights of ricins A, B, and C were about 62,000 and that of ricinus agglutinin was 120,000, estimated by amino acid compositions and SDS gel electrophoresis. They all possessed two non-identical subunits: A and B chains linked by one disulfide bond. Their LD50 values were 4, 28, 14 and 112 micrograms per kg body weight of mice for ricins A, B and C and ricinus agglutinin, respectively. The amino acid compositions of the three toxins and their A and B subunits were very similar, but not identical, while ricinus agglutinin showed a different composition. Ricin A is a newly isolated lectin which has a strong inhibitory effect on the growth of tumor cells. By using cell cultures, it was demonstrated that the tumor cells were more sensitive to lectin than non-transformed cells, and that this could be caused by the higher binding affinity of lectin to tumor cells than to non-transformed cells.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D002367:Ricinus communis / Q000032:analysis*; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D037121:Plant Lectins; D010947:Plants, Toxic; D012276:Ricin / Q000302:isolation & purification* / Q000494:pharmacology; D012278:Ricinus / Q000032:analysis*; D012510:Sarcoma 180 / Q000188:drug therapy
D000596:Amino Acids; D000972:Antineoplastic Agents, Phytogenic; D002241:Carbohydrates; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D012276:Ricin