title: Cytosol glucocorticoid receptor in the neoplastic epithelial duct cell line from human salivary gland (HSG).
abstract: Neoplastic epithelial duct cell line from human salivary gland (HSG cell) contained cytosol glucocorticoid receptor. Scatchard analysis of cytosol indicated that the dissociation constant (Kd) was 5.6-6.5 nmol/l and the number of binding sites was 83-92 fmol/mg protein. A competitive assay showed that the binding sites for [3H]triamcinolone acetonide were specific to glucocorticoid. Glycerol density gradient centrifugation displayed that the [3H]triamcinolone acetonide receptor complexes sedimented in the 8.5 S region under low salt conditions and in the 4.2 S region under high salt condition (0.4 M KCl). The same high salt conditions induced an increased binding of [3H]triamcinolone acetonide complexes for DNA-cellulose.
mesh_terms: D001667:Binding, Competitive; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification / Q000378:metabolism*; D012468:Salivary Gland Neoplasms / Q000378:metabolism*; D014222:Triamcinolone Acetonide / Q000378:metabolism
chemical_list: D011965:Receptors, Glucocorticoid; D014222:Triamcinolone Acetonide