Polyamines permit the preparation of stable Physarum core particles which have a structure similar to those from higher eukaryotes.
The inherent instability of Physarum nucleosome core particles prepared by micrococcal nuclease digestion in Na+/Ca2+ buffers can be overcome by the addition of 0.15 mM spermine and 0.5 mM spermidine. Neutron scattering, circular dichroism, nuclease digestion and thermal denaturation studies carried out on these stable monosomes show them to be very similar to those obtained from higher eukaryotes.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D002942:Circular Dichroism; D006657:Histones / Q000032:analysis; D006358:Hot Temperature; D008836:Micrococcal Nuclease; D009502:Neutrons; D009691:Nucleic Acid Denaturation; D009707:Nucleosomes / Q000032:analysis / Q000648:ultrastructure*; D010804:Physarum / Q000235:genetics / Q000648:ultrastructure*; D011073:Polyamines; D012542:Scattering, Radiation
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D011073:Polyamines; D008836:Micrococcal Nuclease