title: 1,25-Dihydroxyvitamin D3-stimulated mRNAs in rat small intestine.
abstract: The technique of differential hybridization has been employed to study gene expression associated with vitamin D action on the mammalian intestine. A cDNA library consisting of 10(6) independent recombinants was constructed from poly(A)+ RNA extracted from vitamin D-deficient rats given 1,25-dihydroxyvitamin D3. A survey of 20,000 clones resulted in identification of four distinct cDNAs whose corresponding mRNAs are significantly increased 12 h after an intrajugular dose of 1,25-dihydroxyvitamin D3 given to vitamin D-deficient rats. DNA sequence analysis identified these mRNAs as mitochondrial ATP synthetase, vitamin D-dependent calcium binding protein, cytochrome oxidase subunit I, and cytochrome oxidase subunit III. The time course of response of three of these mRNAs was similar, with maximum values at 12 h after dosing, while that of cytochrome oxidase subunit I showed two peaks at 6 and 18 h following a single dose of 1,25-dihydroxyvitamin D3. The levels of all four mRNAs were elevated in rats supplied with vitamin D when hypocalcemia was produced by dietary calcium restriction.
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D005786:Gene Expression Regulation / Q000187:drug effects; D005815:Genetic Code; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000187:drug effects*; D051381:Rats
chemical_list: D012333:RNA, Messenger; D004247:DNA; D002117:Calcitriol; D002118:Calcium