Solubilization of native actin monomers from human erythrocyte membranes.
Up to 50% of the actin in erythrocyte membranes can be solubilized at low ionic strength in a form capable of inhibiting DNAse I, in the presence of 0.4 mM ATP and 0.05 mM calcium. In the absence of calcium and ATP, actin is released but is apparently rapidly denatured. Solubilization of G-actin increases with temperature up to 37 degrees C. At higher temperatures, actin is released rapidly but quickly loses its ability to inhibit DNAse I.
D000199:Actins / Q000097:blood* / Q000494:pharmacology; D000255:Adenosine Triphosphate; D002118:Calcium; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors; D004910:Erythrocyte Membrane / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D011487:Protein Conformation; D012995:Solubility
D000199:Actins; D000255:Adenosine Triphosphate; D003850:Deoxyribonuclease I; D002118:Calcium