title: A protein-tyrosine kinase in the nuclear matrix from rat liver.
abstract: Protein kinase activity in isolated nuclei from rat liver was detected in situ after electrophoresis on SDS-polyacrylamide gel containing no exogenous protein substrate. After renaturation of polypeptides, the gel was incubated with [gamma-32P]ATP and divalent cations. Among five major protein kinase activities observed as radioactive bands by autoradiography, a protein kinase autophosphorylating on tyrosine (Mr 30,000) was identified and found to be localized in the nucleus, particularly in the nuclear matrix. The intensity of the activity band representing the level of the protein-tyrosine kinase in rat liver nuclei did not appreciably change during 3-24 h after partial hepatectomy.
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000201:enzymology* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D011505:Protein-Tyrosine Kinases / Q000032:analysis*; D051381:Rats
chemical_list: D011505:Protein-Tyrosine Kinases