Evaluation of different antigens in a seroepizootiological survey of trichinellosis by enzyme-linked immunosorbent assay.
Antigen isolated from the large-particle fraction of the muscle Trichinella spiralis larvae (PAW), excretory/secretory (E/S) and crude worm extract (CWE) antigens were evaluated in a seroepizootiological survey of trichinellosis by the enzyme-linked immunosorbent assay (ELISA). The ELISA using PAW antigen yielded 16 positive animals (1.6%), E/S antigen revealed 21 (2.1%) positive, and the highest number of positive (23 or 2.3%) were obtained using CWE antigen. Parasitological post-mortem examination of all seropositive animals showed five and seven false-positive animals when E/S and CWE antigens were used, respectively.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000947:Antigens, Helminth; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D000906:Antibodies; D000947:Antigens, Helminth