Kinetics of some immunological and biochemical changes of immunocompetent cells during tumor growth in rats.
The dynamics of lymphocyte response in peripheral blood, the tumor draining lymph node, spleen and thymus, was followed in a model system of syngeneically transplanted rat MC-1 tumor. Electrophoretic mobility (EPM) of lymphoid cells was determined by the automatic mode of measurement. The results revealed a two-phase pattern of EPM changes during the course of cancer growth. The first phase (day 3 to 6 following intramuscular injection of tumor cells) was characterized by a prevalence of high-mobility cells, while in the second phase (day 14 to 20), depletion of high-mobility cells was compensated for by an increased number of low-mobility cells. The mean EPM value was found to be increased only in the thymus at that time. Changes in the adenosine deaminase activity proved to be most expressive in the tumor draining lymph node and in the second phase also in splenic and thymic lymphocytes. An increased percentage of active lymphocytes with compact nucleoli with nucleolonemas became evident already on the 3rd day in all the lymphoid organs followed. The response was two-phasic only in the lymphocyte population of the peripheral blood, while their percentage in the other organs remained higher even on day 20. Changes in the proportion of high- and low-mobility cells in the lymphoid organs followed here, in correlation with the adenosine deaminase activity and the percentage of active lymphocytes, were interpreted as a response of immunocompetent cells in animals with a growing tumor.
D000243:Adenosine Deaminase / Q000032:analysis; D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure; D004586:Electrophoresis; D005260:Female; D008214:Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000502:physiology*; D009374:Neoplasms, Experimental / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D000243:Adenosine Deaminase