title: Regulation of production of embryonic chick liver delta-aminolevulinate synthase: effects of testosterone and of hemin on the mRNA of the enzyme.
abstract: The effects of testosterone and of hemin on the concentration of the mRNA of embryonic chick liver ALA synthase were investigated. Using cDNA-RNA liquid hybridization analyses, we determined that testosterone, when injected into the fluid surrounding chick embryos, caused a dose-dependent increase in the concentration of ALA synthase mRNA in liver. Similarly, addition of testosterone (5 micrograms/ml) or of 75 micrograms/ml of allylisopropylacetamide (AIA) into the medium of chick embryo hepatocytes maintained in culture caused an increase in the concentration of ALA synthase mRNA. Hemin (2 or 5 microM), when added to the culture medium, inhibited the elevations of ALA synthase mRNA concentration brought on by testosterone and by AIA.
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis* / Q000235:genetics; D000502:Allylisopropylacetamide / Q000494:pharmacology; D000818:Animals; D002642:Chick Embryo; D003997:Dicarbethoxydihydrocollidine / Q000494:pharmacology; D006418:Heme / Q000031:analogs & derivatives* / Q000032:analysis; D006427:Hemin / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis*; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D000502:Allylisopropylacetamide; D013739:Testosterone; D006418:Heme; D003997:Dicarbethoxydihydrocollidine; D006427:Hemin; D000624:5-Aminolevulinate Synthetase