title: Absorption of water-miscible forms of vitamin E in a patient with cholestasis and in thoracic duct-cannulated rats.
abstract: Oral administration of vitamin E (100 mg tocopherol X kg-1 X day-1) as tocopheryl polyethylene glycol 1000 succinate (TPGS) to a child with congenital hepatic cholestasis (unresponsive to oral administration of dl-alpha-tocopheryl acetate) promoted an increase of tocopherol in plasma and adipose tissue while tocopheryl acetate emulsified with medium chain triglycerides and polysorbate 80 (MCT-E) did not. alpha-Tocopherol absorption, quantitated in thoracic duct-cannulated rats receiving intraduodenal infusions of soybean oil and saline, was similar for TPGS, MCT-E, and dl-alpha-tocopheryl acetate; gamma-tocopherol absorption from soybean oil was not affected by the presence of the supplemental alpha-tocopherol. Following bile duct ligation in one rat, TPGS promoted the absorption of alpha-tocopherol while absorption of gamma-tocopherol from soybean oil was decreased 30 fold, demonstrating that TPGS, which forms a micellar solution, delivers alpha-tocopherol through the unstirred water layer to enterocytes, while free tocopherol (alpha or gamma) absorption requires the presence of bile salts.
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002404:Catheterization; D002648:Child; D002780:Cholestasis, Intrahepatic / Q000378:metabolism*; D005260:Female; D006801:Humans; D007408:Intestinal Absorption; D051381:Rats; D012995:Solubility; D013897:Thoracic Duct / Q000502:physiology*; D014810:Vitamin E / Q000008:administration & dosage / Q000378:metabolism*; D014867:Water
chemical_list: D014867:Water; D014810:Vitamin E