Proteolysis of arterial basement membrane containing different amounts of carbohydrates.
The arterial basement-membrane-like material was isolated from cultures of rabbit aortic myomedial cells by a sonication/differential-centrifugation technique. Using exoglycosidases the enzymic removal of fucose, glucose, mannose, N-acetylglucosamine and sialic acid resulted in a statistically significant increased susceptibility against the tryptic degradation (2 P less than 0.02), whereas the removal of galactose did not have any effect on the degradation of the basement-membrane-like material. Application of endo-beta-N-acetylglucosaminidase H caused a statistically significant increased sensitivity against trypsin digestion (2 P less than 0.02), whereas the proteolytic degradation of the basement-membrane-like material was unchanged when endo-beta-N-acetylglucosaminidase D was applied.
D000117:Acetylglucosamine / Q000502:physiology; D000818:Animals; D001158:Arteries / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000502:physiology*; D002478:Cells, Cultured; D005643:Fucose / Q000502:physiology; D005947:Glucose / Q000502:physiology; D006026:Glycoside Hydrolases / Q000494:pharmacology; D008358:Mannose / Q000502:physiology; D019158:N-Acetylneuraminic Acid; D011817:Rabbits; D012794:Sialic Acids / Q000502:physiology; D014357:Trypsin / Q000494:pharmacology
D002241:Carbohydrates; D012794:Sialic Acids; D005643:Fucose; D006026:Glycoside Hydrolases; D014357:Trypsin; D019158:N-Acetylneuraminic Acid; D005947:Glucose; D008358:Mannose; D000117:Acetylglucosamine