Immunoaffinity purification of subcellular particles and organelles.
The application of immunoaffinity techniques to subcellular fractionation is reviewed and the basic principles underlying the various methods that have been successfully employed, identified. The requirement for organelle-specific antigens and high-avidity antibodies is discussed, as is the widespread use of indirect immunoadsorbents. Approaches for the optimization of immunoaffinity-based subcellular fractionation are suggested.
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens; D002458:Cell Fractionation / Q000379:methods; D006801:Humans; D007106:Immune Sera; D007202:Indicators and Reagents; D009940:Organoids / Q000648:ultrastructure*; D013347:Subcellular Fractions / Q000648:ultrastructure*
D000936:Antigen-Antibody Complex; D000941:Antigens; D007106:Immune Sera; D007202:Indicators and Reagents