In vivo covalent binding of aminofluorene derivatives to mitochondrial liver DNA of rats pretreated with phenobarbital.
The covalent binding index (CBI) of aminofluorene derivatives (AF) to both nuclear (n) and mitochondrial (mt) hepatic DNA of rats 16 h after i.p. injection of N-acetyl-2-aminofluorene (AAF) has been determined. It was 69 and 212 respectively, i.e. about 3 times higher in mtDNA than in the nDNA. When rats were pretreated for 5 days with phenobarbital (PB) the two CBI decreased in parallel indicating a detoxification of AAF preventing its binding to both nDNA and mtDNA.
D015073:2-Acetylaminofluorene / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D004272:DNA, Mitochondrial; D015073:2-Acetylaminofluorene; D010634:Phenobarbital