title: Early alterations at the plasma membrane of breast cancer cell lines in response to estradiol and hydroxytamoxifen.
abstract: The time course of the early stage of estradiol-17 beta (E2) and hydroxytamoxifen (OHTAM) action at the plasma membrane of hormone-responsive MCF-7 and non-responsive MDA-MB-231 (MDA) breast cancer cell lines was investigated using scanning electron microscopy (SEM), electron probe X-ray microanalysis and microelectrophysiology analysis. SEM showed a marked increase in the density and the length of microvilli (MV) on MCF-7 cells treated with 1 nM estradiol for 1 min. This membrane response disappeared at 5 min. No early effect was obtained with OHTAM, but both compounds produced a similar surge of heterogeneous MV at 15 min of treatment. The morphological change induced by E2 subsided at 60 min, whereas that of OHTAM persisted. X-ray microanalysis and computer determination of peak/background ratios permitted the demonstration that these morphological alterations were concomitant with a rise in the intracellular level of potassium. Microelectrophysiology analysis showed a sharp transitory decrease in the membrane potential of MCF-7 cells in response to estradiol. In the estrogen-insensitive MDA cells, the hormone did not modify the membrane potential and K levels decreased at 1 and 5 min before rising again to control levels at minute 15 when MV appeared. With OHTAM, potassium decreased significantly at 60 min of treatment. These initial and transitory changes in surface morphology paralleled by alterations in potassium level may be consistent with the occurrence of estrogen membrane receptors on target cells, a new aspect of steroid hormone action.
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology* / Q000503:physiopathology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D004577:Electron Probe Microanalysis; D004594:Electrophysiology; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D008564:Membrane Potentials; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000473:pathology; D011188:Potassium / Q000378:metabolism; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D013629:Tamoxifen; C016601:afimoxifene; D004958:Estradiol; D011188:Potassium