Enzymatic conversion of 7-hydroxyguanine to its nucleosides.
7-Hydroxyguanine, an antitumor purine-analog, reacted with ribose (or deoxyribose)-1-phosphate in the presence of purine nucleoside phosphorylase. We prepared 7-hydroxyguanine riboside and deoxyriboside using purine nucleoside phosphorylase from bovine spleen. Each nucleoside exhibits antitumor activity against mouse leukemia L1210 cells. The therapeutic effect of 7-hydroxyguanine deoxyriboside is superior to those of 7-hydroxyguanine and 7-hydroxyguanine riboside.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D055598:Chemical Phenomena; D002621:Chemistry; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D009705:Nucleosides / Q000494:pharmacology*
D000970:Antineoplastic Agents; D009705:Nucleosides; C046682:7-hydroxyguanine; D006147:Guanine