The structure and formation of the glucose 6-phosphate adduct of hemoglobin A: a 31P-NMR study.
The glucose 6-phosphate adduct of hemoglobin formed on deoxy incubation of the sugar with hemoglobin is primarily present in solution as the unstable aldimine compound; in contrast, the percent ketoamine is higher if the adduct is formed in the presence of carbon monoxide. The adduct has a 31P nuclear magnetic resonance peak with a chemical shift which is 0.7 ppm up-field from the shift of unreacted glucose 6-phosphate at pH 7.0 and is constant between pH 6 and 8, while the unreacted sugar phosphate shows the characteristic change of chemical shift due to ionization of one of the phosphate protons. This suggests that, in the adduct, phosphate is involved in a salt bridge, probably at the 2,3-diphosphoglyceric acid binding site.
D019794:2,3-Diphosphoglycerate; D000588:Amines; D002248:Carbon Monoxide / Q000494:pharmacology; D002845:Chromatography; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D004355:Drug Stability; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism*; D006441:Hemoglobin A / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007097:Imines; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000378:metabolism; D012996:Solutions
D000588:Amines; D004163:Diphosphoglyceric Acids; D005958:Glucosephosphates; D006454:Hemoglobins; D007097:Imines; D010710:Phosphates; D012996:Solutions; D019794:2,3-Diphosphoglycerate; D019298:Glucose-6-Phosphate; D002248:Carbon Monoxide; C100003:deoxyhemoglobin; D006441:Hemoglobin A