[Separation of glycogen phosphorylase isoenzymes using electrophoresis in acetate cellulose].
A new electrophoretic procedure is developed for estimation of glycogen phosphorylase isoforms using acetate cellulose films. In the course of incubation of a mixture containing substrate the reaction was directed towards formation of glucose-I-phosphate, concentration of which was estimated in the coupled reactions based on equimolar increase in content of NADH. Content of the reduced nucleotide was evaluated by means of fluorescent densitometry. Three fractions of glycogen phosphorylase I, II and III were detected after electrophoretic separation of an extract from heart muscle homogenate. Only the isoenzyme I was found in blood serum of patients with myocardium infarction. In subsequent studies clinical validity of the procedure should be evaluated in order to prove the suitability of glycogen phosphorylase isoenzymes estimations for diagnostics of myocardial infarction.
D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000097:blood*; D009206:Myocardium / Q000201:enzymology*; D006005:Phosphorylases / Q000032:analysis / Q000097:blood*
D007527:Isoenzymes; D006005:Phosphorylases
Razdelenie izoform glikogenfosforilazy metodom élektroforeza na atsetate tselliulozy.