Carcinogenic effects in A/J mice of particulate of a coal tar paint used in potable water systems.
Coal tar paints are among the products used as inside coatings for water pipes and storage tanks to retard corrosion in potable water supply systems. Four different formulations of these paints were tested in earlier work by this laboratory in the Ames mutagenesis and the mouse skin carcinogenesis bioassays. The paint most active in these assays were then tested in a particulate form in the lung adenoma assay with A/J mice. The paint was applied to clean glass plates, cured, collected and homogenized in 2% Emulphor. Doses of this coal tar suspension were administered by gavage at 1.0, 10.0 and 55.0 mg in 0.2 ml per mouse 3X weekly for 8 weeks. The total doses of coal tar paint were 24, 240, and 1320 mg/mouse. Benzo[a]pyrene (BaP), administered in a parallel schedule to a total dose of 6 mg/mouse, served as positive control. A negative control group received an equivalent volume of 2% Emulphor. Animals were killed at 9 months of age (8 months after first dose) and lung adenomas counted. A dose-related response, in the average number of lung tumors per mouse, was observed with the coal tar particulate. There were also squamous cell tumors of the forestomach in 42% of the mice receiving 55.0 mg coal tar paint per application.
D000818:Animals; D003033:Coal Tar / Q000633:toxicity*; D005260:Female; D008175:Lung Neoplasms / Q000139:chemically induced; D051379:Mice; D009374:Neoplasms, Experimental / Q000209:etiology*; D010150:Paint / Q000032:analysis / Q000633:toxicity*; D013274:Stomach Neoplasms / Q000139:chemically induced; D014881:Water Supply / Q000032:analysis*
D003033:Coal Tar