Oxidation of low-density lipoprotein by thiol compounds leads to its recognition by the acetyl LDL receptor.
Reduced glutathione and other compounds with free -SH groups promoted the oxidation of low-density lipoprotein (LDL) in the absence of cells in Ham's F-10 medium. In contrast, compounds in which the thiol groups were oxidized or blocked were ineffective in oxidizing LDL. Thiol-induced modification of LDL did not occur in media lacking in redox metals. It is suggested that thiols react with redox metal, generating thiol- and oxygen-derived free radicals that promote modification of LDL.
D000818:Animals; D015815:Cell Adhesion Molecules; D003545:Cysteine / Q000494:pharmacology; D004727:Endothelium / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011817:Rabbits; D011973:Receptors, LDL / Q000378:metabolism*; D051116:Receptors, Scavenger; D013438:Sulfhydryl Compounds / Q000502:physiology*
D015815:Cell Adhesion Molecules; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D051116:Receptors, Scavenger; D013438:Sulfhydryl Compounds; D005978:Glutathione; D003545:Cysteine