The effect of Mg2+ and calmidazolium on the calmodulin binding to inside-out vesicles of the human red cell membrane.
Calmodulin binds to inside-out vesicles of the human red cell membrane in a Ca2+-dependent manner. Mg2+ was found to be essential to this binding, half maximum binding occurring at decreasing Ca2+ concentration with increasing Mg2+ concentration. The calmodulin antagonist calmidazolium strongly reduced the Ca2+-dependent binding of calmodulin to the inside-out vesicles.
D001665:Binding Sites; D002147:Calmodulin / Q000097:blood*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D007700:Kinetics
D002147:Calmodulin; D007093:Imidazoles; C031938:calmidazolium