A new immunization procedure for the obtention of anti-leucine enkephalin antibodies. Part I. Immunization procedure and physicochemical characteristics of antibodies.
We described here a new immunization procedure to obtain high titre and high specific antibodies against Leu-enkephalin (LE). The immunogen form is composed of one part of LE conjugate and one part of LE-Arg6 conjugate. We have observed an increase of titre, affinity and specificity of the antibodies in the coimmunization procedure compared to those obtained by conventional immunization involving only the LE conjugate. The Leu-enkephalin antibodies exhibit a high affinity (KD 8 X 10(-12) M) and we are able to detect the Leu-enkephalin at the 10(-15) mole level. These LE antibodies are highly specific of the C part of LE peptide and cross-react weakly with Met-enkephalin (1%).
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D004743:Enkephalin, Leucine / Q000032:analysis / Q000276:immunology*; D007106:Immune Sera / Q000032:analysis; D007114:Immunization / Q000379:methods*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D004743:Enkephalin, Leucine