Studies on fatty acid-binding proteins. The detection and quantification of the protein from rat liver by using a fluorescent fatty acid analogue.
Fatty acid-binding protein from rat liver is shown to bind the fluorescent fatty acid probe dansyl undecanoic acid. Binding is accompanied by a shift in the fluorescence emission maximum from 550 nm to 500 nm and a 60-fold fluorescence enhancement at 500 nm. These spectral properties have allowed the use of this probe to detect and quantify microgram amounts of liver fatty acid-binding protein during purification procedures. In conjunction with h.p.l.c. the method allows the rapid estimation of liver fatty acid-binding protein in biological samples. The validity of the method is demonstrated by measuring the concentration of fatty acid-binding protein in livers from control and hypolipidaemic-drug-treated rats. The dramatic diurnal rhythm previously reported for this protein [Dempsey (1984) Curr. Top. Cell. Regul. 24, 63-86] was not observed with this method.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid; D002940:Circadian Rhythm; D002995:Clofibric Acid / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003619:Dansyl Compounds; D004591:Electrophoresis, Polyacrylamide Gel; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005233:Fatty Alcohols / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D002352:Carrier Proteins; D003619:Dansyl Compounds; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005233:Fatty Alcohols; D000960:Hypolipidemic Agents; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C004568:tiadenol; D002995:Clofibric Acid; C051042:dansyl undecanoic acid