A modified method for the estimation of galactose-1-phosphate.
The measurement of galactose-1-phosphate has been advocated for monitoring galactosaemia. A radioenzymatic method is described in which red-cell haemolysates are incubated with radiolabelled uridine diphospho-glucose and exogenous galactose-1-phosphate uridyl transferase. Labelled glucose-1-phosphate is formed in proportion to the amount of galactose-1-phosphate present and is separated from the labelled uridine diphospho-glucose by affinity chromatography. The method is reproducible and has a low limit of detection. This allows it to be carried out on 100 microL of packed red cells.
D001897:Boronic Acids; D002846:Chromatography, Affinity; D004912:Erythrocytes / Q000032:analysis; D005695:Galactosephosphates / Q000097:blood*; D005782:Gels; D006600:Hexosephosphates / Q000097:blood*; D006801:Humans
D001897:Boronic Acids; D005695:Galactosephosphates; D005782:Gels; D006600:Hexosephosphates; C029973:galactose-1-phosphate