title: Production and characterization of a monoclonal antibody to biotin.
abstract: Monoclonal antibodies to biotin have been prepared by using biotin linked to keyhole limpet haemocyanin (KLH) as the antigen. Spleen cells obtained from mice immunized with biotin-KLH were fused with the myeloma cell line NS-1. The resulting hybridomas were screened for the production of antibodies to biotin using an enzyme-linked immunosorbent assay. Clones producing antibodies to biotin were isolated by limiting dilution methods. Four cell lines, each derived originally from a different fusion, were chosen for the production of monoclonal antibodies. The monoclonal antibodies obtained have been characterized with respect to their ability to interact with biotin, biotin-bovine serum albumin, biotin-KLH and biocytin as well as to inhibit biotin-dependent enzymes. They have been used to produce cellular biotin deficiency in vitro for studies of biotin function.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification*; D000918:Antibody Specificity; D001202:Ascitic Fluid / Q000276:immunology; D001710:Biotin / Q000031:analogs & derivatives / Q000276:immunology*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors; D005260:Female; D006367:HeLa Cells; D006801:Humans; D043586:Methylmalonyl-CoA Decarboxylase; D051379:Mice; D008807:Mice, Inbred BALB C; D014176:Protein Biosynthesis
chemical_list: D000911:Antibodies, Monoclonal; D001710:Biotin; D002262:Carboxy-Lyases; D043586:Methylmalonyl-CoA Decarboxylase