Occurrence of premature chromosome condensation in mouse spermatogonia treated with busulfan.
Busulfan induction of premature chromosome condensation (PCC) was examined in mouse spermatogonia. Adult (C3H X SWV) F1 male mice were injected intraperitoneally with busulfan at 10 or 30 mg/kg of body weight and killed 18-72 h later. Polyploid-like mitoses were frequent (ca. 1/4 of all spermatogonial mitoses examined) in both the untreated and treated groups. Most of these were considered to have been derived from normal spermatogonia. In busulfan-treated mice, polyploid-like mitoses with PCC were seen. The frequency of PCC-containing mitoses increased with increasing dose and exposure time. A possible interpretation for PCC induction in mouse spermatogonia is discussed.
D000818:Animals; D002066:Busulfan / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000187:drug effects*; D007621:Karyotyping; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D011003:Ploidies; D013093:Spermatogonia / Q000187:drug effects* / Q000648:ultrastructure; D013094:Spermatozoa / Q000187:drug effects*
D002066:Busulfan