Protection against paraquat-induced injury by exogenous GSH in pulmonary alveolar type II cells.
Exogenous GSH provided rat alveolar type II cells with significant protection against injury induced by paraquat. This protection was also observed in cells treated with acivicin to inhibit GSH degradation and buthionine sulfoximine to inhibit GSH synthesis. Exogenous GSH was transported into cells by a Na+-dependent system. Addition of inhibitors of this transport system, gamma-glutamyl-glutamate and probenecid, prevented the protection against injury afforded by GSH. Thus, the results indicate that alveolar type II cells can supplement endogenous synthesis of GSH with uptake of exogenous GSH to protect against paraquat-induced injury.
D000818:Animals; D001692:Biological Transport; D019328:Buthionine Sulfoximine; D004151:Dipeptides / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism / Q000627:therapeutic use*; D007555:Isoxazoles / Q000494:pharmacology; D007700:Kinetics; D008171:Lung Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control; D008297:Male; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D010269:Paraquat / Q000633:toxicity*; D011339:Probenecid / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology
D004151:Dipeptides; D007555:Isoxazoles; C047442:gamma-glutamylglutamine; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D012964:Sodium; D005978:Glutathione; C006303:acivicin; D010269:Paraquat; D011339:Probenecid