title: Kurloff cell proteoglycans. Evidence for de novo synthesis of chondroitin sulphate proteoglycans by purified Kurloff cells.
abstract: This paper reports the first direct demonstration of de novo synthesis of chondroitin sulphate proteoglycans by Kurloff cells. This was achieved using highly purified splenic Kurloff cells labelled in vitro with [35S]sulphate and D-[U-3H]glucosamine. A single population of sulphated proteoglycans was observed after dissociative extraction, DEAE-cellulose chromatography, Sepharose CL 6B chromatography and fluorography after electrophoresis. These were large, highly anionic proteoglycans and were completely digested by chondroitinase AC or ABC. Moreover, glycosaminoglycan extracted from Kurloff cells had the electrophoretic mobility of control chondroitin sulphate.
mesh_terms: D000818:Animals; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D005944:Glucosamine / Q000378:metabolism; D006168:Guinea Pigs; D008970:Molecular Weight; D011509:Proteoglycans / Q000096:biosynthesis*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014316:Tritium; D005944:Glucosamine