title: [Structural characteristics of integral protein-3 of rat erythrocyte membranes in hypovitaminosis D].
abstract: The amino acid composition, lipid-binding ability, the values of pK and binding capacity of negatively charged sites of the integral protein-3 from erythrocyte membranes are determined for rats in norm and under conditions of D-hypovitaminosis. Protein-3 binding of phosphatidyl choline and sphingomyelin considerably decreases, while that of cholesterol and lanosterol increases with the unchanged amino acid composition under conditions of D-hypovitaminosis. This leads to the enhancement of the protein electro-negativity as its carboxylic groups and histidine imidazole become more accessible for titration. An increase of the negative charge of protein-3 with D-hypovitaminosis is supposed to be responsible for inhibition of the anionic transport realized by it through the forces of electrostatic repulsion.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis*; D002855:Chromatography, Thin Layer; D004910:Erythrocyte Membrane / Q000032:analysis*; D007700:Kinetics; D008563:Membrane Lipids / Q000097:blood; D008565:Membrane Proteins / Q000097:blood*; D010455:Peptides / Q000032:analysis; D051381:Rats; D014808:Vitamin D Deficiency / Q000097:blood*
chemical_list: D000596:Amino Acids; D001457:Anion Exchange Protein 1, Erythrocyte; D008563:Membrane Lipids; D008565:Membrane Proteins; D010455:Peptides
vernacular_title: Strukturnye osobennosti integral'nogo belka-3 membran érytrotsitov krys pri D-gipovitaminoze.