With which component of the bovine isolated cornea does sodium lauryl sulphate react to produce opacity?
Several concentrations of sodium lauryl sulphate (NaLS) were applied to bovine corneas which were either intact or had the epithelium or endothelium or both removed. The opacities which developed in the corneas were in the order epithelium and endothelium removed greater than endothelium removed greater than epithelium removed greater than intact. Opacities increased with increasing concentrations of NaLS from 5 X 10(-4) M to 5 X 10(-2) M, but at 5 X 10(-1) M all corneal preparations showed a decrease in opacity. It is suggested that the opacity may be due to a reaction between NaLS and stromal proteins.
D000818:Animals; D002417:Cattle; D003318:Corneal Opacity / Q000139:chemically induced*; D004727:Endothelium / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D066298:In Vitro Techniques; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity*
D012967:Sodium Dodecyl Sulfate