Nitropyrene-induced DNA repair in Clara cells and alveolar type-II cells isolated from rabbit lung.
DNA excision repair, as measured by unscheduled DNA synthesis (UDS), was examined in different cell types of rabbit lung exposed to nitropolycyclic aromatic hydrocarbons (NO-PAH) in vitro. Dose-related increases in UDS were observed. 1,6-Dinitropyrene (1,6-DNP) and 1,8-dinitropyrene (1,8-DNP) induced UDS more effectively in alveolar type-II cells compared with Clara cells. On the other hand, 1-nitropyrene (1-NP) caused a weak UDS response in Clara cells but no DNA repair in alveolar type-II cells.
D000818:Animals; D004260:DNA Repair / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008297:Male; D011721:Pyrenes / Q000494:pharmacology*; D011817:Rabbits
D011721:Pyrenes; C033310:1,8-dinitropyrene; C033309:1,6-dinitropyrene; C032668:1-nitropyrene