[Intraperitoneal cavity injection of OK-432 with fresh human serum in a case of carcinomatous peritonitis--determination on the complement-derived chemotactic factor].
Large numbers of neutrophils appear in the peritoneal cavity within a few hours after administration of OK-432 into the peritoneal cavity, and play an important role in the destruction of cancer cells. As OK-432 can activate complement in vitro, it is possible that a chemotactic factor such as C5a is generated and attracts neutrophils into the cavity. We injected OK-432 with fresh human serum preincubated at 37 degrees C for 60 min in order to generate large amounts of complement-derived chemotactic factor. Neutrophils increased in number within a few hours after the injection of OK-432 with preincubated human serum into the peritoneal cavity of a recurrent gastric cancer patient with carcinomatous ascites, and persisted much longer compared with treatment using OK-432 alone. Cancer cells rapidly decreased in number and ascites disappeared two weeks after the treatment with OK-432 and preincubated human serum. It was found that C5a levels measured by RIA initially increased a few hours before neutrophils appeared, and then decreased gradually, after injection of either OK-432 with preincubated human serum or with OK-432 alone. Although complement-derived chemotactic factor might be responsible for attracting neutrophils into the peritoneal cavity, the role of other chemotactic factors should also be investigated.
D000230:Adenocarcinoma / Q000276:immunology* / Q000628:therapy; D001688:Biological Products / Q000008:administration & dosage*; D002630:Chemotactic Factors / Q000276:immunology*; D003167:Complement Activation; D006801:Humans; D007116:Immunization, Passive; D007267:Injections; D009504:Neutrophils / Q000276:immunology; D010529:Peritoneal Cavity; D010538:Peritonitis / Q000276:immunology* / Q000628:therapy; D010844:Picibanil / Q000008:administration & dosage*; D013274:Stomach Neoplasms / Q000276:immunology* / Q000628:therapy
D001688:Biological Products; D002630:Chemotactic Factors; D010844:Picibanil