High-performance liquid chromatographic assay for morphine with electrochemical detection using an unmodified silica column with a non-aqueous ionic eluent.
A quick, sensitive and accurate high-performance liquid chromatographic method for morphine measurement in plasma has been developed using an unmodified silica column with a non-aqueous ionic eluent. Small plasma samples (0.4 ml) were extracted with a liquid-liquid extraction column and dihydromorphine was used as internal standard. The limit of quantitation was 2 ng/ml. The electrochemical detector was capable of detecting 150 pg of morphine. A calibration line with a correlation of 0.9994 +/- 0.00026 was produced over the range 2-80 ng/ml.
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004090:Dihydromorphine / Q000097:blood; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D009020:Morphine / Q000032:analysis* / Q000097:blood
D007202:Indicators and Reagents; D009020:Morphine; D004090:Dihydromorphine