Determination of the kinetic constants of inhibited acetyl cholinesterase.
A method for the determination of kinetic constants of inhibited acetylcholinesterase, in the presence of substrate, is proposed. Theoretical, experimental and data treatment aspects are described for a suitable analysis of inhibitors acting with one or two steps. Limitations of other methods recently reported are pointed out.
D000110:Acetylcholinesterase / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D007700:Kinetics
D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase