[Changes in the sorption properties of LDH protein in the rat brain after the single administration of a formaldehyde solution].
The study of the activity, protein charge and isoenzyme LDG spectrum after a single injection of formaldehyde solution has demonstrated a rise in the protein negative charge, which in the absence of ischemia was observed for 20 min. A more than 5-min increase in LDG protein charge observed in ischemia and independent of changes in LDG isoenzyme spectrum was significantly less expressed in ischemic brain after a preliminary injection of formaldehyde solution. With cerebral blood flow preserved, an injection of formaldehyde resulted in the alteration of LDG spectrum, namely a rise in LDG3+4+5 content. Formaldehyde injection prior to ischemia prevented the development of a similar effect, which was significantly more expressed in ischemic brain without a preliminary formaldehyde injection.
D000042:Absorption; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002545:Brain Ischemia / Q000201:enzymology; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D051381:Rats; D012996:Solutions; D013997:Time Factors
D007527:Isoenzymes; D012996:Solutions; D005557:Formaldehyde; D007770:L-Lactate Dehydrogenase
Izmenenie sorbtsionnykh svoĭstv belka LDG mozga krys posle odnokratnogo vvedeniia rastvora formal'degida.