Characterization of haem disorder by circular dichroism.
Native and reconstituted myoglobin were prepared and their c.d. spectra recorded in the Soret region. Time-dependent changes in dichroism following reconstitution were observed and related to haem orientational disorder. Comparative c.d. studies, in agreement with n.m.r. studies, reveal that the degree and nature of this disorder are species-dependent.
D002942:Circular Dichroism; D006418:Heme / Q000032:analysis; D009211:Myoglobin; D011487:Protein Conformation; D013053:Spectrophotometry
D009211:Myoglobin; C018013:carboxymyoglobin; D006418:Heme