Acetylcholinesterase is not inhibited by pyridoxal 5'-phosphate.
Acetylcholinesterase activity was assayed in the absence and presence of pyridoxal 5'-phosphate. If substrate hydrolysis was measured by the pH-stat method, its rate was not significantly affected by pyridoxal 5'-phosphate. In the spectrophotometric assay, however, this compound led to an apparent decrease in rate. The discrepancy between the two assays is explained by stray-light artefacts produced by pyridoxal 5'-phosphate at the wavelengths of the spectrophotometric assay.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors; D007700:Kinetics; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D051381:Rats; D013053:Spectrophotometry
D002800:Cholinesterase Inhibitors; D011732:Pyridoxal Phosphate; D000110:Acetylcholinesterase