title: Increased fucosylation of chick brain proteins following training: effects of cycloheximide.
abstract: When chicks are trained to avoid pecking a bead coated with methylanthranilate in a one-trial passive avoidance task there is an increase in fucose incorporation in vivo and in vitro in the right forebrain base of methylanthranilate (M)-trained compared to water (W)-trained chicks. The relation of this increase to de novo protein synthesis in vivo and in vitro has been examined. Cycloheximide (Cx), 1 mM, inhibited in vitro fucosylation of chick brain slices by 60% after 3 h. However, the training-related increase in in vitro fucosylation still persisted. When Cx was injected intraventricularly 10 min before training, the subsequent increase in in vitro fucosylation due to training was still apparent. When Cx was injected and [14C]leucine and [3H]fucose incorporation studied in vivo in M-trained and W-trained chicks, there was no increase in fucosylation due to training in the Cx-treated M-trained over the W-trained chicks. These results are taken to indicate that in vitro fucosylation and its increase subsequent to training is not protein synthesis-dependent, but that both in vivo and in vitro there are interactions between Cx and fucosylation steps that are independent of Cx's effects on protein synthesis.
mesh_terms: D000818:Animals; D001362:Avoidance Learning; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002645:Chickens; D003216:Conditioning, Operant; D003513:Cycloheximide / Q000494:pharmacology*; D005643:Fucose / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D005643:Fucose; D003513:Cycloheximide; D007930:Leucine