Purification and partial characterization of DNA-dependent RNA polymerase from Rhodomicrobium vannielii.
DNA-dependent RNA polymerase has been isolated from Rhodomicrobium vannielii. Like those from other eubacteria, the enzyme contained four subunits: beta and beta prime (Mr 155,000), alpha (Mr 38,000), and sigma (Mr 98,000). Analysis by isoelectric focussing showed that both alpha and sigma had several forms with different isoelectric pH values. The enzyme was sensitive to rifampicin (5 ng rifampicin ml-1 gave 50% inhibition) and capable of specific promoter selection with DNA from R. vannielii, calf thymus and phage T7D111.
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D008970:Molecular Weight; D012244:Rhodospirillaceae / Q000201:enzymology* / Q000235:genetics; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D046911:Macromolecular Substances; D012321:DNA-Directed RNA Polymerases