title: Induction of antibiotic production with ethidium bromide in Streptomyces hygroscopicus.
abstract: Protoplast regeneration carried out in a carriomycin producing organism, Streptomyces hygroscopicus 358 AV2, lose carriomycin productivity without loss of carriomycin-resistance and the ability of formation of aerial mycelium. Ethidium bromide treatment on the 358 AV2 strain generated a bald mutant that produced carriomycin and a new antibiotic curromycin. In some other media, however, the parent strain produced curromycin, indicating that the ethidium bromide treatment altered the regulation of antibiotic production. Ethidium bromide treatment on a protoplast-regenerated strain derived from the parent strain resulted in derivatives capable of producing carriomycin and curromycin. These strains were unstable and tended to lose the recovered antibiotic productivity easily.
mesh_terms: D000165:Acridine Orange / Q000494:pharmacology; D000167:Acriflavine / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media; D004271:DNA, Fungal / Q000187:drug effects; D004996:Ethidium / Q000494:pharmacology*; D010080:Oxazoles / Q000096:biosynthesis; D053284:Polyunsaturated Alkamides; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D011714:Pyrans / Q000096:biosynthesis; D013302:Streptomyces / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004271:DNA, Fungal; D010080:Oxazoles; D053284:Polyunsaturated Alkamides; D011714:Pyrans; D000167:Acriflavine; C016290:carriomycin; C046081:curromycin A; D004996:Ethidium; D000165:Acridine Orange