Intralipid administration induces a lipoprotein lipase-like activity in the livers of starved adult rats.
The administration of Intralipid to starved adult rats induces the appearance of lipoprotein lipase (LPL)-like activity in the liver, whereas the so-called hepatic triacylglycerol lipase is unaffected. This LPL-like activity is eluted by 1.5 M-NaCl from heparin-Sepharose columns. This partially purified fraction is inhibited by 1.0 M-NaCl (91%) and by 1.0 mg of protamine sulphate/ml (79%), whereas it is stimulated 69-fold by the presence of 8.0 micrograms of apolipoprotein C-II/ml and inhibited by anti-LPL antibodies. We conclude that Intralipid administration induces the appearance of LPL activity in livers of starved adult rats. Its possible origin is discussed.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001053:Apolipoproteins / Q000494:pharmacology; D002846:Chromatography, Affinity; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology*; D008066:Lipolysis; D008071:Lipoprotein Lipase / Q000276:immunology / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D011479:Protamines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D013217:Starvation / Q000201:enzymology; D014280:Triglycerides / Q000097:blood
D000906:Antibodies; D001053:Apolipoproteins; D005217:Fat Emulsions, Intravenous; D011479:Protamines; D014280:Triglycerides; D012965:Sodium Chloride; D008071:Lipoprotein Lipase