Effects of levels of feed restriction on in vivo and in vitro alterations in drug metabolism and associated enzymes.
The objective of this study was to determine if 15% and 30% feed restriction would enhance drug metabolism similar to that caused by 45% feed restriction. Four groups of weanling male Sprague-Dawley rats were subjected to 0%, 15%, 30%, and 45% feed restriction for 28 days. Feed restriction of 45% significantly enhanced hepatic DNA, microsomal protein, cytochrome P-450, aniline hydroxylase, p-chloromethylaniline-N-demethylase, and decreased hexobarbital sleeping time. The feed restriction of 15% and 30% did not significantly alter the above parameters of drug metabolism. The activities of NADPH-generating enzymes were enhanced by all levels of feed restriction. Thus it is concluded that 15% and 30% feed restriction did not enhance in vivo or in vitro drug metabolism to the extent enhanced by 45% restriction for the same length of time.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004032:Diet; D006591:Hexobarbital / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012890:Sleep / Q000187:drug effects
D004364:Pharmaceutical Preparations; D011506:Proteins; D004247:DNA; D006591:Hexobarbital; D006899:Mixed Function Oxygenases