Expression of lectin receptors on the surface of granulocyte-macrophage progenitor cells (CFU-gm).
It has been emphasized that specific bindings between membrane glycoproteins and membrane lectin-like substances are important in cell-to-cell interactions. We explored the surface of granulocyte-macrophage precursor cells (CFU-gm) by the differential agglutination technique. Enrichment of CFU-gm in the agglutinated fraction, containing the cells which have lectin receptors, from marrow treated with soybean agglutinin (SBA), peanut agglutinin (PNA) and concanavalin A (Con A), suggests the presence of reactive galactosyl and mannosyl residues on the surface of CFU-gm. On the other hand, wheat germ agglutinin (WGA), phytohemagglutinin (PHA) and ulex europaeus agglutinin (UEA), which bind to reactive N-acetylglucosamine, N-acetylgalactosamine and fucose, respectively, did not specifically agglutinate CFU-gm. Thus, reactive groups containing galactosyl and mannosyl structures on the surface of CFU-gm may possibly play a role in the process of cell-to-cell interactions between CFU-gm and marrow stromal cells.
D000818:Animals; D002449:Cell Aggregation; D002450:Cell Communication; D002462:Cell Membrane / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006098:Granulocytes / Q000276:immunology* / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000502:physiology; D008264:Macrophages / Q000276:immunology* / Q000502:physiology; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D011971:Receptors, Immunologic / Q000502:physiology*
D006023:Glycoproteins; D008565:Membrane Proteins; D011971:Receptors, Immunologic