Human alveolar macrophages synthesize the functional alternative pathway of complement and active C5 and C9 in vitro.
Attachment of protein to agarose beads cultured with macrophages in protein-free medium containing 3H-leucine, shows that de novo synthesis of protein with affinity to the beads takes place. We also found that monoclonal antibodies against human C3c, C3g, and a C9-neoantigen as well as polyclonal antibodies against human C5 and C9, bound to agarose beads that had been kept with the macrophage cultures. Demonstration of C3 derivatives on the agarose beads shows that the essential complement factors of the alternative pathway are synthesized and have been activated by the beads. Deposition of C5 and the detection of a neoantigen of C9 on the beads, indicates that the whole terminal complement pathway has been formed and activated. We conclude that human alveolar macrophages form in vitro the functional alternative pathway of complement, C5 and C9, and we have indirect evidence for synthesis of C6, C7, and C8.
D000911:Antibodies, Monoclonal / Q000276:immunology; D003167:Complement Activation; D003176:Complement C3 / Q000096:biosynthesis / Q000276:immunology; D003182:Complement C5 / Q000096:biosynthesis* / Q000276:immunology; D003186:Complement C9 / Q000096:biosynthesis* / Q000276:immunology; D003170:Complement Pathway, Alternative; D003165:Complement System Proteins / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000378:metabolism*; D012685:Sepharose / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003176:Complement C3; D003182:Complement C5; D003186:Complement C9; D003165:Complement System Proteins; D012685:Sepharose