title: Studies on the correlation of a range of immunoassays for diphtheria antitoxin with the guinea-pig intradermal test.
abstract: The diphtheria antitoxin titres determined in human sera by the intradermal titration method have been compared with the titres obtained by a range of ELISA assays. A direct ELISA based on highly purified diphtheria toxoid correlated poorly with the in vivo method but an assay based on capture of toxoid by a non-neutralizing monoclonal antibody produced results that correlated very well. The performance of assays involving partial blockade of antigen and non-neutralizing monoclonal antibodies was intermediate.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001681:Biological Assay / Q000592:standards; D004166:Diphtheria Antitoxin / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D009500:Neutralization Tests; D011863:Radioimmunoassay; D012882:Skin Tests / Q000592:standards
chemical_list: D000911:Antibodies, Monoclonal; D004166:Diphtheria Antitoxin