Isolation and characterization of an endogenous drug-binding inhibitor present in uremic serum.
Isolation of an endogenous ligand solute from uremic serum was performed by high-performance liquid chromatography. This ligand solute inhibited the binding of diphenylhydantoin and tryptophan to plasma protein, and was considered to be one of the endogenous drug-binding inhibitors present in uremic serum. The gas chromatographic-mass spectrometric analysis revealed that this inhibitor is 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid, which is known to be a constituent of urine.
D005663:Furans; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008024:Ligands / Q000302:isolation & purification; D010672:Phenytoin / Q000378:metabolism; D011422:Propionates / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D014364:Tryptophan / Q000378:metabolism; D014511:Uremia / Q000097:blood*
D005663:Furans; D008024:Ligands; D011422:Propionates; D010672:Phenytoin; C041693:3-carboxy-4-methyl-5-propyl-2-furanpropionic acid; D014364:Tryptophan