Uridine enhances the cytotoxic effect of D-glucosamine in rat C6 glioma cells.
This paper studies the influence of uridine on the effects exerted by D-glucosamine in rat C6 glioma cells. 2 mM uridine increased markedly both the cytotoxic effect of the aminosugar and the inhibition of thymidine incorporation into acid-insoluble fraction. Furthermore the complete resumption of the capacity to incorporate either 3H-thymidine or 3H-mannose which was observed after the removal of the aminosugar, was impeded when the cells were treated contemporaneously with D-glucosamine and uridine. An exposure for 4 hr to 20 mM glucosamine alone enhanced about 15-fold the cellular pool of UDP-N-acetylhexosamines; the addition of 2 mM uridine intensified the expansion of this pool, which became about 35-fold the control value. The findings suggest a connection between the accumulation of UDP-N-acetylhexosamines in the cells and the appearance of D-glucosamine cytotoxicity.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D005910:Glioma / Q000473:pathology*; D005944:Glucosamine / Q000494:pharmacology*; D008358:Mannose / Q000378:metabolism; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000494:pharmacology*; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D014537:Uridine Diphosphate N-Acetylglucosamine; D005944:Glucosamine; D008358:Mannose; D013936:Thymidine; D014529:Uridine