Occurrence of Gal beta (1----3) GalNAc-Ser/Thr in the linkage region of polygalactosamine containing fungal glycoprotein from Cordyceps ophioglossoides.
The nitrous acid deamination liberated the N-acetyl galactosamine containing glycoproteins from galactosminoglycan (CO-N) which has been isolated from Cordyceps ophioglossoides culture. Mild alkaline borohydride degradation of the purified glycoprotein released oligosaccharide alditols. The smallest oligosaccharide alditol was characterized to be Gal beta (1----3)-GalNAcol. This result indicated that polygalactosamine part attached to protein part via Gal beta (1----3) GalNAc-Ser/Thr as the linkage region. To our knowledge, this is the first report of Gal-GalNAc-Ser/Thr linkage from fungal glycoprotein.
D005656:Fungal Proteins / Q000032:analysis*; D005688:Galactosamine / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006999:Hypocreales / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis*
D005656:Fungal Proteins; D006023:Glycoproteins; D009844:Oligosaccharides; D005688:Galactosamine