title: Influence of oxygen radicals generated by xanthine oxidase in the isolated perfused rat heart.
abstract: To investigate the cardiac effects of enzymatically generated oxygen radicals isolated Langendorff rat heart preparations were perfused with hypoxanthine (0.96 mmol X litre-1) plus xanthine oxidase (0.025 U X ml-1). Oxygen radicals produced an immediate increase in coronary flow. After 10 min a pronounced reduction in contractile performance, as well as in concentrations of high energy phosphates, was seen. Electron microscopical examination showed damage with cellular oedema as a prominent finding. These effects were all effectively reversed by the specific enzymes, superoxide dismutase and catalase, proving that they were due to oxygen radicals.
mesh_terms: D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects; D005609:Free Radicals; D006321:Heart / Q000187:drug effects*; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D010100:Oxygen / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014969:Xanthine Oxidase / Q000494:pharmacology
chemical_list: D005609:Free Radicals; D014969:Xanthine Oxidase; D010100:Oxygen