Excretion of benzo[a]pyrene-Gua adduct in the urine of benzo[a]pyrene-treated rats.
A benzo[a]pyrene(BP)-Gua adduct was extracted in the urine of rats treated with BP. Some (0.15%) of the administered dose of BP was excreted as BP-Gua within 48 h. A double labelling experiment demonstrated that the excreted product contained both a BP and a Gua moiety. Partially hepatectomized rats treated with [14C]Gua during the regenerative phase were injected with [3H]BP and the urine collected and processed by chromatographic procedures. The adduct had similar chromatographic properties to the adduct released from human PLC/5 cells treated with 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) and co-chromatographed with 7-BPDE-Gua released from BPDE-adducted DNA under aqueous conditions. Detection and quantitation of BP-Gua offers an alternative, non-invasive method of monitoring individuals exposed to carcinogenic polycyclic aromatic hydrocarbons (PAHs).
D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D001580:Benzopyrenes / Q000378:metabolism / Q000652:urine*; D002460:Cell Line; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D001580:Benzopyrenes; D001564:Benzo(a)pyrene; D006147:Guanine; C051092:7-(7,8,9-trihydroxy-7,8,9,10-tetrahydrobenzo(a)-pyren-10-yl)guanine