Challenge reactions in atopic dermatitis after percutaneous entry of mite antigen.
We succeeded in reproducing an eczematous lesion on the apparently normal skin of a patient with atopic dermatitis by scratching and continuous application of an ointment containing ferritin-labelled mite antigen. Percutaneous entry of mite antigen was demonstrated in skin biopsies by Fe-staining. Scratched skin first showed an urticarial reaction typical of type I allergy which later changed into an eczematous reaction typical of type IV allergy. This change was also shown histologically.
D000328:Adult; D000485:Allergens / Q000276:immunology*; D039741:Antigens, Dermatophagoides; D003876:Dermatitis, Atopic / Q000276:immunology*; D006801:Humans; D007159:Immunologic Tests; D008297:Male; D008925:Mites / Q000276:immunology*; D011537:Pruritus / Q000276:immunology; D012882:Skin Tests
D000485:Allergens; D039741:Antigens, Dermatophagoides