Demonstration of a plasmid-borne gene encoding a thermostable direct hemolysin in Vibrio cholerae non-O1 strains.
Of 15 Vibrio cholerae non-O1 strains, 2 produced a hemolysin termed NAG-rTDH, which is very similar to the thermostable direct hemolysin of V. parahaemolyticus. These two strains contained DNA sequences which are homologous to a DNA probe for the V. parahaemolyticus thermostable direct hemolysin gene. A probe-positive 9-kilobase HindIII fragment was cloned from a plasmid of a V. cholerae non-O1 strain into plasmid pBR322, and the resulting Escherichia coli clones produced intracellular NAG-rTDH.
D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D014734:Vibrio cholerae / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; D006460:Hemolysin Proteins