title: Effects of the volatile anesthetic halothane on fertilization and early development in the sea urchin Lytechinus variegatus: evidence that abnormal development is due to polyspermy.
abstract: The volatile anesthetic halothane, when present at fertilization, dose-dependently increases the incidence of abnormally developing sea urchin embryos at the first cell division. Microscopic examinations of eggs stained with aceto-orcein or the DNA fluorochrome bisbenzimide and direct observations on isolated sperm aster complexes show that halothane induces polyspermy (multiple sperm entry) when present at fertilization. Experimental evidence suggests that anesthetic-induced polyspermy involves impairment of both the fast (electrically mediated) and slow (morphological) blocks to multiple sperm entry. These observations clearly show that relatively brief exposures to halothane at fertilization cause polyspermy and that this effect is almost certainly responsible for the ensuing abnormal development observed at the first cell division.
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D006221:Halothane / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D012617:Sea Urchins / Q000196:embryology*; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D015053:Zygote / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D006221:Halothane