[Change in the initiation of DNA synthesis on the nuclear matrix and its DNA-protein composition in gamma-irradiated hepatoma cells].
It was shown that gamma-irradiation of Zajdela hepatoma cells (10 Gy) induces inhibition of DNA synthesis initiation at a nuclear matrix and a change in its DNA-protein content. Irradiation of hepatoma cells with 10 and 50 Gy decreases incorporation of newly synthesized proteins in the firmly bound DNA-protein complexes of nuclear matrix. After 60-120 min postirradiation incubation of cells at 37 degrees C DNA-protein content of the nuclear matrix and its firmly bound DNA-protein complexes are restored. However the rate of DNA synthesis initiation being below the control level.
D000818:Animals; D003037:Cobalt Radioisotopes; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D005720:Gamma Rays; D066298:In Vitro Techniques; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011831:Radiation Genetics; D051381:Rats
D003037:Cobalt Radioisotopes; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins
Izmenenie initsiatsii sinteza DNK na iadernom matrikse i ego DNK-belkogo sostava v gamma-obluchennykh kletkakh gepatomy.