title: Purification and properties of the DNA invertase gin encoded by bacteriophage Mu.
abstract: The host range of bacteriophage Mu is regulated through an invertible segment. Inversion requires the presence of two properly oriented recombination sites and a recombinational enhancer sis. The reaction is catalyzed by the Mu-encoded DNA invertase Gin and a host factor termed factors for inversion stimulation (FISs). We present a novel purification scheme for Gin. Purified Gin alone catalyzes the inversion reaction at very low efficiency recombining less than 0.8% of substrate molecules. When supplemented with FIS substrates containing the recombinational enhancer are recombined efficiently. Stoichiometric amounts of Gin are required for recombination.
mesh_terms: D002413:Cations, Divalent; D002845:Chromatography; D002850:Chromatography, Gel; D007446:Chromosome Inversion; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000302:isolation & purification* / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000302:isolation & purification*
chemical_list: D002413:Cations, Divalent; D004279:DNA, Viral; D014764:Viral Proteins; D008274:Magnesium