[Localization of beta-glucuronidase in the kidney of the white rat after administration of the antidiuretic hormone].
Distribution of beta-glucuronidase, one of three enzymes of hyaluronate hydrolases (HH) that hydrolyze extracellular glycosamine glycans (GAG), has been studied in the intact white rat kidney and under effect of antidiuretic hormone (ADH). Renal beta-glucuronidase is revealed as discrete granules in cytoplasm of epithelial cells, in the cortex and medulla and in the interstitial cells of the papilla. ADH increase for a short time in blood results in decreasing amount of granules in cytoplasm of epithelial cells of the collecting tubules, and a prolonged increase is accompanied with practically a complete disappearance of granules both in cytoplasm of epitheliocytes of the collecting tubules and in spindle-like interstitial cells. This effect is considered as a result of the enzyme discharge under ADH effect from the cells into the interstitial space--the place of the extracellular GAG position, HH action substrate. The possibility of the enzyme presence in the renal cells in the form of two fractions is discussed.
D000818:Animals; D003594:Cytoplasmic Granules / Q000201:enzymology; D005966:Glucuronidase / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D008297:Male; D051381:Rats; D014667:Vasopressins / Q000494:pharmacology*
D006025:Glycosaminoglycans; D014667:Vasopressins; D005966:Glucuronidase
Lokazlizatsiia beta-gliukuronidazy v pochke beloĭ krysy v usloviiakh deĭstviia antidiureticheskogo gormona.