Direct evidence for the involvement of domain III in the N-F transition of bovine serum albumin.
The domain III of bovine serum albumin containing residues 377-582 of the protein sequence was isolated and its behaviour in acid solution was studied. The fragment was found to undergo structural transformations over the pH range 3.5-4.5 known to cause N-F transition in serum albumin. On the other hand, an albumin fragment that was devoid of domain III was unable to exhibit such a transition. These results were consistent with a mechanism where N-F transition involves the separation of domain III from the rest of the albumin starts at about pH 4.3 and is completed at pH 3.5.
D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010446:Peptide Fragments; D011487:Protein Conformation; D012710:Serum Albumin, Bovine
D010446:Peptide Fragments; D012710:Serum Albumin, Bovine