Subunit-exchange chromatography of self-associating proteins: a quantitative reappraisal.
A quantitative expression describing the behavior of a self-associating protein in subunit-exchange chromatography is derived in a form that is tractable from the viewpoint of characterizing the pertinent interactions. Its use is illustrated by application to published results for alpha-chymotrypsin, oxyhemoglobin, and the light-harvesting chlorophyll a/b protein.
D002734:Chlorophyll / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D002918:Chymotrypsin / Q000302:isolation & purification; D010108:Oxyhemoglobins / Q000302:isolation & purification; D011487:Protein Conformation; D011506:Proteins / Q000302:isolation & purification*
D010108:Oxyhemoglobins; D011506:Proteins; D002734:Chlorophyll; D002918:Chymotrypsin