title: Glycolipid modulation of membrane adhesion.
abstract: A novel test of carbohydrate-mediated adhesion has been developed. At the tips of two syringes, large spherical model membranes have been made from phosphatidylcholine and varying amounts of mixed brain gangliosides dissolved in n-decane. The apposition of two such membranes resulted in adhesion, not fusion, as judged by the absence of fluorescence mixing in the junction with NBD-phosphatidylethanolamine in one membrane and perylene in the other. Adhesion was observed without gangliosides. The rate of formation of the adhesion area ("rate" of adhesion) was unchanged from 0 to 0.8 mol% gangliosides. A slightly lower but constant rate was observed within the physiological range from 2 to 10 mol%. Adhesion was frequently blocked at 11 to 15 mol% gangliosides. The rate of adhesion with pure gangliosides increased with the number of sialic acid residues: GT greater than GD1a greater than GM1. These results are interpreted in terms of a sialic acid-dependent segregation of gangliosides into the adhesion zone.
mesh_terms: D000268:Adhesiveness; D005732:Gangliosides / Q000378:metabolism*; D008561:Membrane Fusion; D008567:Membranes, Artificial; D010713:Phosphatidylcholines / Q000378:metabolism*
chemical_list: D005732:Gangliosides; D008567:Membranes, Artificial; D010713:Phosphatidylcholines