Nerve growth factor effect on adenosine transport in cultured chromaffin cells.
Chromaffin cells both recently isolated or in culture present a high-affinity adenosine transporter with a Km value of 1 microM. When cells were exposed to nerve growth factor (NGF; 10 ng/ml), the adenosine transporter affinity decreased to 3 microM. This value was maintained from 3 days after plating to the end of the culture period. A change in the transport capacity was observed, with a significant increase (approximately 200-260%) in NGF-cultured cells throughout the period studied.
D000241:Adenosine / Q000378:metabolism*; D001692:Biological Transport; D002478:Cells, Cultured; D002838:Chromaffin System / Q000166:cytology / Q000378:metabolism*; D007700:Kinetics; D009414:Nerve Growth Factors / Q000494:pharmacology*
D009414:Nerve Growth Factors; D000241:Adenosine