The amino-terminal domain of rabbit secretory component is responsible for noncovalent binding to immunoglobulin A dimers.
Rabbit secretory components (SC) constitute a family of markedly heterogeneous glycoproteins which are released in the secretions as free SC or as SC bound to polymeric immunoglobulins. The aim of this work was to determine the region of the SC polypeptides which is involved in IgA binding. The high and the low Mr forms of free SC (or IgA-dissociated bound SC) and the native secretory IgA complex were subjected to limited tryptic digestion. Chemically characterized peptides ranging in apparent size from 15 to 20 kDa, depending upon the allotype, were shown to be necessary and sufficient for efficient noncovalent binding to IgA dimers (subclass g). These fragments encompass the amino-terminal first domain of SC, i.e. residues 1-126, when aligned with the predicted amino acid sequence from a cDNA clone encoding the rabbit polymeric Ig receptor (Mostov, K.E., Friedlander, M., and Blobel, G. (1984) Nature 308, 37-43). The high and the low Mr forms of SC exhibited the same relative affinity for IgA dimers, suggesting that the postulated internal deletion in the smaller polypeptide (Kühn, L. C., Kocher, H.-P., Hanly, W.C., Cook, L., Jaton, J.-C., and Kraehenbuhl, J.-P. (1983) J. Biol. Chem. 258, 6653-6659) does not impair the IgA dimer recognition function.
D000595:Amino Acid Sequence; D000818:Animals; D002918:Chymotrypsin; D005260:Female; D007070:Immunoglobulin A / Q000302:isolation & purification / Q000378:metabolism*; D007128:Immunoglobulin Fragments / Q000302:isolation & purification; D046911:Macromolecular Substances; D008892:Milk / Q000276:immunology; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011247:Pregnancy; D011485:Protein Binding; D011817:Rabbits; D012635:Secretory Component / Q000302:isolation & purification; D014357:Trypsin
D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D046911:Macromolecular Substances; D010446:Peptide Fragments; D012635:Secretory Component; D002918:Chymotrypsin; D014357:Trypsin