title: Sensitive and specific bromocriptine radioimmunoassay with iodine label: measurement of bromocriptine in human plasma.
abstract: Plasma bromocriptine assays must have high sensitivity because plasma concentrations are low, and high specificity because bromocriptine is extensively metabolised. This paper describes the simple preparation of a radioiodine-labelled derivative of dihydroergocriptine and its use in a radioimmunoassay employing an antiserum directed against the intact bromocriptine molecule. The method could measure plasma bromocriptine at concentrations of 0.05 nmol/L, had between-assay coefficients of variation of less than 10% and was more convenient than previous assays using tritium radiolabels.
mesh_terms: D000236:Adenoma / Q000097:blood / Q000188:drug therapy; D001971:Bromocriptine / Q000097:blood* / Q000627:therapeutic use; D006801:Humans; D007457:Iodine Radioisotopes; D010911:Pituitary Neoplasms / Q000097:blood / Q000188:drug therapy; D011388:Prolactin / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods*
chemical_list: D007457:Iodine Radioisotopes; D001971:Bromocriptine; D011388:Prolactin