Biochemical and histochemical studies of alkaline and acid phosphatases in a digenetic trematode, Pegosomum egretti.
The biochemistry and histochemistry of Pegosomum egretti have been studied using standard techniques. Phosphatases were analysed colorimetrically; the optimum pH for acid phosphatase activity was 5.0 and for alkaline phosphatase was 10.0. The results were compared with those of other trematodes. Histochemical localization of acid and alkaline phosphatases revealed differences in enzymes activity in various tissues. These differences in the site and pattern of distribution of the two enzymes have been discussed in relation to transport of raw materials and the metabolism of the cell concerned.
D000135:Acid Phosphatase / Q000032:analysis*; D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D001717:Birds / Q000469:parasitology; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D014200:Trematoda / Q000201:enzymology*
D000469:Alkaline Phosphatase; D000135:Acid Phosphatase