Sodium-independent binding of L-[3H]proline to hippocampal synaptic membranes.
L-[3H]Proline binds specifically and saturably to rat hippocampal synaptic membranes in the absence of inorganic ions. Saturation isotherms constructed in Hepes-Tris buffer best fit a model of a single independent population of binding sites with a dissociation constant of 1.6 microM and maximum density of 16 pmol/mg protein. The rank order of displacement of radioligand by proline analogues suggests that binding sites of this type could be receptors responsible for proline-induced retinal spreading depression and inhibition of memory consolidation.
D000818:Animals; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D011392:Proline / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000502:physiology*; D013570:Synaptic Membranes / Q000378:metabolism*
D011392:Proline; D012964:Sodium