Potentiation of collagen synthesis in explants of the rabbit eye by 5 beta-dihydrocortisol.
The biologic effect of 5 beta-dihydrocortisol on collagen synthesis was evaluated. The metabolite was found to potentiate subthreshold levels of dexamethasone in increasing 3H-proline incorporation in cells of the outflow region of the rabbit. Digestion of the tissue with highly purified collagenase indicated that the 3H-proline was incorporated into collagen type protein. This study demonstrates another biologic activity of 5 beta-dihydrocortisol, a metabolite found to accumulate in cells cultured from trabeculectomy specimens from patients with primary open angle glaucoma.
D000818:Animals; D001345:Autoradiography; D003094:Collagen / Q000096:biosynthesis*; D005123:Eye / Q000378:metabolism*; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D011392:Proline / Q000378:metabolism; D011817:Rabbits; D014316:Tritium
D014316:Tritium; C018921:5-dihydrocortisol; D003094:Collagen; D011392:Proline; D006854:Hydrocortisone