Cholesterol effects on the interaction of cardiolipin with anti-cardiolipin antibody.
Human antibodies to cardiolipin, phosphatidic acid and phosphatidylserine were assessed by binding to nitrocellulose paper and subsequent reaction with an enzyme-linked or radioactively labelled second antibody to human IgG. The addition of cholesterol to constant amounts of cardiolipin impregnated in the nitrocellulose paper resulted in a profound fall in antibody binding beginning at a 0.5 to 1 molar ratio of cholesterol to cardiolipin and stabilizing at about 15% of the original level. Antibody binding to phosphatidic acid and phosphatidylserine also showed extensive cholesterol-induced inhibition beginning at a slightly lower molar ratio of cholesterol to phospholipid. The structural array of neither the cardiolipin alone impregnated in nitrocellulose nor the phospholipid together with cholesterol is known. It is possible that the specific cardiolipin phase structure required for human antibody recognition was disrupted by cholesterol.
D001323:Autoantibodies; D002308:Cardiolipins / Q000276:immunology*; D002784:Cholesterol / Q000494:pharmacology*; D006801:Humans; D010712:Phosphatidic Acids / Q000276:immunology; D010718:Phosphatidylserines / Q000276:immunology; D013587:Syphilis / Q000276:immunology*
D001323:Autoantibodies; D002308:Cardiolipins; D010712:Phosphatidic Acids; D010718:Phosphatidylserines; D002784:Cholesterol