Sister chromatid exchange analysis in lung and peripheral blood lymphocytes of mice exposed to methyl isocyanate by inhalation.
Mice were exposed to 1, 3, or 6 ppm methyl isocyanate (MIC) for 6 hr/day for four consecutive days. Lung cells and peripheral blood lymphocytes (PBLs) were removed and cultured for analysis of sister chromatid exchange (SCE) and cell cycle kinetics. MIC caused a small but significant increase in SCE frequency of cultured lung cells from mice exposed to 1, 3, or 6 ppm MIC. MIC did not significantly increase SCE levels in PBLs of mice exposed to concentrations as high as 6 ppm. In cultured PBLs, MIC had a stimulatory effect on cell cycling rates as measured by the replicative index, and it caused a significant reduction in mononuclear leucocyte counts and the mitotic indices.
D000280:Administration, Inhalation; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D003485:Cyanates / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D017953:Isocyanates; D008168:Lung / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012737:Sex Factors; D012854:Sister Chromatid Exchange
D003485:Cyanates; D017953:Isocyanates; C008461:methyl isocyanate