Protein migration through hydrogels: a tool for measuring porosity--application to hydrogels used as contact lenses.
A rapid two-dimensional electrophoretic system for evaluating the penetration of proteins into hydrogels (used as contact lenses) is presented. The proteins are sieved through the hydrogel under conditions which produce minimum deformity of pores. In another step, the proteins migrating through the pores are identified. An effective pore size measurement is deduced.
D003263:Contact Lenses, Hydrophilic; D004586:Electrophoresis; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D011506:Proteins
D011506:Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols