Microspectrofluorometric comparison of benzo(a)pyrene and dibenzo(c,h)acridine metabolism in single living 3T3 fibroblasts.
The purpose of this study was to determine if dibenzo(c,h)acridine could be used as a probe for the N-heterocyclic aromatic compound detoxification system. The determination was achieved by cell population studies (histograms) using microspectrofluorimetry on single living cells. The results show a similar metabolic distribution pattern between dibenzo(c,h)acridine and benzo(a)pyrene, whereas important differences appear between 6-aminochrysene and dibenzo(c,h)acridine, respectively amine aromatic and N-heterocyclic aromatic compounds. No differences were observed in the metabolism of benzo(a)pyrene in untreated cells and those treated with constant concentrations of dibenzo(c,h)acridine. However, inhibition of dibenzo(c,h)acridine metabolism was observed in the presence of benzo(a)pyrene, indicating that this compound should be a preferential substrate for a cell detoxification system.
D000166:Acridines / Q000378:metabolism*; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002460:Cell Line; D002911:Chrysenes; D005347:Fibroblasts / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008832:Microchemistry / Q000379:methods; D010616:Phenanthrenes / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods
D000166:Acridines; D002911:Chrysenes; D010616:Phenanthrenes; C022289:dibenzacridine; D001564:Benzo(a)pyrene; C100101:6-chrysenamine