Visualization and analysis of mural thrombogenesis on collagen, polyurethane and nylon.
Visualization and analysis of mural thrombogenesis on collagen-coated glass, polyurethane and nylon was discussed. Epi-fluorescent video microscopy was used to visualize thrombotic events at a protein or polymer surface in contact with flowing whole blood. Digital image processing was used to analyse the real-time microscopic images obtained, resulting in measurements of morphological features and the number of platelets that compose each thrombus. The three-dimensional structures of the thrombi were also estimated. A photodiode was used to measure integrated end-point platelet accumulation at different axial positions along the surface. Additionally, the convection-diffusion equations were solved to estimate the concentrations of adenosine diphosphate, thromboxane A2, and thrombin generated by activated platelets at the blood-contacting surface.
D000244:Adenosine Diphosphate / Q000096:biosynthesis; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000502:physiology; D003094:Collagen; D006801:Humans; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D009757:Nylons; D011140:Polyurethanes; D012815:Signal Processing, Computer-Assisted; D013917:Thrombin / Q000096:biosynthesis; D013927:Thrombosis / Q000209:etiology*; D013929:Thromboxane B2 / Q000096:biosynthesis
D001672:Biocompatible Materials; D009757:Nylons; D011140:Polyurethanes; D013929:Thromboxane B2; D000244:Adenosine Diphosphate; D003094:Collagen; D013917:Thrombin