Alpha 1 acid glycoprotein in streptozotocin diabetic rats. Some aspects of sugar moiety structure and metabolism.
The pure alpha 1 acid glycoprotein (AGP) preparation from streptozotocin diabetic rat sera showed diminished sialic acid content and lower ratios of galactose to mannose and galactose to fucose. In vivo incorporation of 14C-leucine and 3H-galactose into AGP of control and diabetic animals was studied 30, 60 and 120 min after administration of the radioisotopic precursors. The changed 14C/3H ratio in AGP of diabetic rats suggested disturbed glycosylation of AGP in this disease. Similar activity of UDP-galactose 4-epimerase in control and diabetic rat liver was found suggesting that this enzyme has no influence on the decreased level of serum protein bound galactose.
D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005260:Female; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D019158:N-Acetylneuraminic Acid; D009961:Orosomucoid / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000032:analysis
D002241:Carbohydrates; D009961:Orosomucoid; D012794:Sialic Acids; D005643:Fucose; D019158:N-Acetylneuraminic Acid; D008358:Mannose; D005690:Galactose