Purification and properties of the DNA invertase gin encoded by bacteriophage Mu.
The host range of bacteriophage Mu is regulated through an invertible segment. Inversion requires the presence of two properly oriented recombination sites and a recombinational enhancer sis. The reaction is catalyzed by the Mu-encoded DNA invertase Gin and a host factor termed factors for inversion stimulation (FISs). We present a novel purification scheme for Gin. Purified Gin alone catalyzes the inversion reaction at very low efficiency recombining less than 0.8% of substrate molecules. When supplemented with FIS substrates containing the recombinational enhancer are recombined efficiently. Stoichiometric amounts of Gin are required for recombination.
D002413:Cations, Divalent; D002845:Chromatography; D002850:Chromatography, Gel; D007446:Chromosome Inversion; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000302:isolation & purification* / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000302:isolation & purification*
D002413:Cations, Divalent; D004279:DNA, Viral; D014764:Viral Proteins; D008274:Magnesium