High performance liquid chromatography in studies of radiolabeled antibodies.
High performance liquid chromatography (HPLC) as applied to the separation of antibodies displays the same advantages as in its other applications, namely good resolution accompanied by fast analysis. It is therefore not surprising that many HPLC columns designed for use with antibodies and other proteins are now available commercially. The properties of proteins which provide the separation are size, hydrophobicity, charge and affinity. The features of each are discussed.
D000906:Antibodies / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007204:Indium; D004369:Pentetic Acid; D011868:Radioisotopes
D000906:Antibodies; D011868:Radioisotopes; D007204:Indium; D004369:Pentetic Acid