Aldehyde dehydrogenase catalyses acetaldehyde formation from 4-nitrophenyl acetate and NADH.
Incubation of sheep liver cytoplasmic aldehyde dehydrogenase with the substrate 4-nitrophenyl [14C]acetate in the presence of NADH leads to the formation of 14C-labelled acetaldehyde. This observation strongly supports the idea that the esterase and dehydrogenase activities of the enzyme occur at the same site and involve the intermediacy of a common acyl-enzyme.
D000079:Acetaldehyde / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000818:Animals; D002855:Chromatography, Thin Layer; D009243:NAD / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism*; D012663:Semicarbazides; D012756:Sheep
D009596:Nitrophenols; D012663:Semicarbazides; D009243:NAD; C010059:carbamylhydrazine; C008642:4-nitrophenyl acetate; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde