title: Clonal chromosome abnormalities in chronic lymphocytic leukemia patients revealed by TPA stimulation of whole blood cultures.
abstract: Cells from 52 patients with chronic lymphocytic leukemia and two patients with prolymphocytic leukemia were stimulated in whole blood or bone marrow culture with the polyclonal B-cell mitogen tetradecanoyl phorbol acetate (TPA). Stimulation was successful in 50 of the 54 cases. Comparison with Epstein-Barr virus and pokeweed mitogen showed them to be much less successful mitogens under the conditions used. Twenty-one patients (39%) were found to have clonal abnormalities that were probably disease-related. The most common abnormality was trisomy 12 (five patients). Four patients had a deletion of the long arm of chromosome #11 and one patient had a t(11;14). Five patients had abnormalities of the long arm of chromosome #13, with four probably having breaks in band q14. Two patients were seen with trisomy 3 as the only abnormality. Overall, the results were similar to those of other groups, which shows that considerable karyotypic information can be obtained from the use of a single mitogen on a large series of chronic lymphocytic leukemia patients.
mesh_terms: D000368:Aged; D001853:Bone Marrow / Q000648:ultrastructure; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000235:genetics*; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D008875:Middle Aged; D008934:Mitogens; D009857:Oncogenes; D011379:Prognosis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D008934:Mitogens; D013755:Tetradecanoylphorbol Acetate