title: A contribution to the quantitative evaluation of yeast cultures stored in liquid nitrogen.
abstract: Different ways of the evaluation of viability of cultures stored in liquid nitrogen are compared. The streaking technique is recommended for a rapid evaluation. It is sufficient to determine the suitability of the medium, of cryopreservatives and of the whole procedure. On storage of melted cultures for 1-7 d at 4 degrees C it was found that the cultures could be transported directly in a suitable package maintaining the low temperature on the condition that the transportation would not last longer than 5 d.
mesh_terms: D005615:Freezing; D009584:Nitrogen; D013997:Time Factors; D015003:Yeasts / Q000254:growth & development*
chemical_list: D009584:Nitrogen