Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors.
We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times between 3 and 14 days provides optimal cellular resolution, whereas long-term autoradiography with 1-2 months of exposure may be used to obtain topographic-regional surveys of distribution.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry; D004958:Estradiol / Q000031:analogs & derivatives*; D005069:Evaluation Studies as Topic; D005260:Female; D007457:Iodine Radioisotopes; D010902:Pituitary Gland / Q000032:analysis* / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000032:analysis*; D014599:Uterus / Q000032:analysis* / Q000166:cytology
D007457:Iodine Radioisotopes; D011960:Receptors, Estrogen; D004958:Estradiol; C024871:16 alpha-iodoestradiol