A sensitive bioluminescence assay for myo-inositol.
A specific, sensitive bioluminescence assay for myo-inositol is described employing myo-inositol dehydrogenase linked to a commercial NADH bioluminescence kit. Optimum conditions providing a linear response over 4 orders of magnitude are presented with reproducibility of 6% CV and a sensitivity of 1 pmol. The assay is suitable for the measurement of inositol at low concentrations or in small volumes.
D007294:Inositol / Q000032:analysis*; D008156:Luciferases; D008163:Luminescent Measurements; D009243:NAD; D013401:Sugar Alcohol Dehydrogenases
D009243:NAD; D007294:Inositol; D013401:Sugar Alcohol Dehydrogenases; C021448:myo-inositol 2-dehydrogenase; D008156:Luciferases