Ultrastructure of ultraviolet radiation-induced hairless mouse skin carcinogenesis, with special reference to the epidermal-dermal junction.
The ultrastructure of ultraviolet (UV)-induced skin pathology was studied in mice to complement previously reported gross and light microscopic findings, and to assess further the usefulness of the animal model for study of sunlight associated epidermal tumours in man. Hairless albino (HRA/Skh-1) mice were exposed to a minimal erythemal dose from a filtered light source emitting both UVA and UVB, approximating solar emission. Samples of normal and hyperplastic skin, pedunculated papillomas, carcinomas in situ and invasive squamous cell carcinomas were processed for transmission electron microscopy once their identity was confirmed by light microscopic examination. Keratinocyte pleomorphism became more marked and cell to cell contact diminished as malignancy developed. For papillomas, carcinomas in situ and invasive squamous cell carcinomas, there was a progressive disruption of the epidermal junction which became marked upon frank invasion. Most of the differences between the various categories of pathological change, therefore, were not absolute but rather of degree, supporting the notion that invasive squamous cell carcinoma represents an end stage for malignancy which may arise de novo, directly from hyperplastic skin, or proceed from other tumour types. The similarity in structure of the mouse tumours to comparable tumours in man supports the usefulness of the animal model and suggests that the results have implications for sunlight associated tumours in man.
D000818:Animals; D002278:Carcinoma in Situ / Q000648:ultrastructure*; D002294:Carcinoma, Squamous Cell / Q000648:ultrastructure*; D004817:Epidermis / Q000648:ultrastructure; D005260:Female; D006965:Hyperplasia / Q000473:pathology; D051379:Mice; D008812:Mice, Hairless; D009381:Neoplasms, Radiation-Induced / Q000648:ultrastructure*; D010212:Papilloma / Q000648:ultrastructure*; D012867:Skin / Q000473:pathology / Q000648:ultrastructure*; D012878:Skin Neoplasms / Q000648:ultrastructure*; D014466:Ultraviolet Rays