Monoclonal antibodies based sandwich erythro-immunoassay and 'dot' enzyme immunoassay for human chorionic gonadotropin in urine.
Two simple solid-phase sandwich immunoassays for human chorionic gonadotropin (hCG) employing monoclonal antibodies have been described. One is a sandwich erythro-immunoassay employing V-shaped well microtitration plates coated with monoclonal anti-beta-hCG antibody and monoclonal anti-alpha-hCG antibody labelled sheep erythrocytes. The second is a 'dot' enzyme immuno-assay employing dip-stick (plastic strips pasted with nitrocellulose pads) coated with monoclonal anti-beta-hCG antibody. Anti-alpha-hCG monoclonal-alkaline phosphatase conjugate was used to reveal hCG bound to solid surface. The assays can be performed by 'one-step' or 'two-step' procedures. Erythro-immunoassay as well as 'dot' enzyme immunoassay was able to detect in urine as low as 10 mIU hCG/ml. A good correlation was observed between the values obtained by these two methods as well as 'two-step' sandwich enzyme immunoassay on 47 urine samples.
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin / Q000652:urine*; D003101:Collodion; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D011247:Pregnancy; D011259:Pregnancy Tests, Immunologic / Q000379:methods*
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D003101:Collodion