title: Caffeine potentiation of calcium release in frog skeletal muscle fibres.
abstract: The effects of caffeine at concentrations up to 3 mM were studied on Ca signals obtained using the metallochromic Ca indicator dyes Arsenazo III and Antipyrylazo III in cut frog skeletal muscle fibres mounted in a triple Vaseline-gap chamber and stimulated by voltage clamp or action potential. The peak amplitude of the transient absorbance change due to Ca2+ release following action potential stimulation is potentiated by an amount dependent on caffeine concentration up to 0.5 mM, and by a concentration-independent amount between 0.5 and 2 mM. At 3 mM-caffeine, the potentiation is reduced, and the Ca signal can have a smaller amplitude than under the control condition. The time course of the rising phase of the Ca signal is preserved by the potentiating effect of caffeine; however, the decay rate of the Ca signal is increasingly slowed at caffeine concentrations greater than 0.5 mM. No substantial change was found in the resting myoplasmic Ca2+ level at caffeine concentrations near 0.5 mM. Even if the free Ca2+ concentration in the presence of this level of caffeine were to increase by 0.04 microM (the threshold of detectability), the calculated potentiation of the Ca signal due to increased partial saturation of intracellular Ca2+ buffers would amount to only about 7%. This value is significantly less than the amount of potentiation observed (up to 40%) following action potentials at caffeine levels of 0.5 mM and above. Experiments made with the impermeant potentiometric dye NK2367 show no alteration by caffeine of the electrical properties of the tubular system. Caffeine at up to moderate concentrations causes a substantial increase in the maximal Ca2+ release obtained following large depolarizations. The voltage dependence of the Ca2+ release is characterized by a caffeine concentration-dependent shift towards more negative membrane potentials. The potentiation of Ca2+ release by caffeine was found to be independent of the external free Ca2+ level. The potentiation of the Ca2+ release process by caffeine is likely to occur at a step subsequent to the depolarization of the transverse tubule membrane, and suggests the presence of an intermediate step, or second messenger, in the excitation-contraction coupling process.
mesh_terms: D000200:Action Potentials; D000818:Animals; D001150:Arsenazo III; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D066298:In Vitro Techniques; D009132:Muscles / Q000378:metabolism*; D009282:Naphthalenesulfonates; D009532:Nickel / Q000494:pharmacology; D010081:Oxazolone / Q000031:analogs & derivatives; D013268:Stimulation, Chemical; D013997:Time Factors
chemical_list: D009282:Naphthalenesulfonates; D010081:Oxazolone; D001150:Arsenazo III; D002110:Caffeine; C025144:NK 2367; D009532:Nickel; C016238:antipyrylazo III; D002118:Calcium