Establishment of a human pancreatic adenocarcinoma cell line (PSN-1) with amplifications of both c-myc and activated c-Ki-ras by a point mutation.
A human pancreatic cancer cell line, PSN-1, was established from pancreatic adenocarcinoma tissue that had been stored for 1.5 years at -80 degrees C without any special treatment. The stored tissues were first transplanted into nude mice, and from the xenograft, the PSN-1 cell line was established. The original primary tumor and two metastatic lymph nodes were previously found to have 50-fold amplification of c-myc and also 3- to 6-fold amplification of activated c-Ki-ras with a point mutation from GGT to CGT at codon 12. PSN-1 cells are unique in that amplifications of both c-myc and activated c-Ki-ras are present in the same degree as the original tumors. These cells were also found to contain increased amounts of c-myc and c-Ki-ras transcripts.
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D002460:Cell Line; D005784:Gene Amplification; D006801:Humans; D051379:Mice; D009154:Mutation; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011519:Proto-Oncogenes; D014183:Transplantation, Heterologous