Mitogenic heparin-binding lectin-like protein from cloned thymic myoid cells.
A mitogenic heparin-binding (reactive) lectin-like protein (HBP) was purified from the extract of a cloned rat thymic myoid cell R615B2 by a one-step procedure of affinity chromatography on a heparin--Sepharose CL-6B column. Four distinct peptide bands with molecular weights of 10,000, 13,000, 13,700, and 14,600 were detected on SDS-polyacrylamide gel electrophoresis. This protein is mitogenic at concentrations of as low as 1.1-70.0 ng/ml for peanut lectin-nonagglutinated thymocytes and splenocytes from euthymic mice and rats but not for splenocytes from nude mice. These results indicate that thymic myoid cell-derived HBP is an important signal for one particular step in T-cell differentiation.
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D006493:Heparin / Q000378:metabolism; D037102:Lectins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000032:analysis / Q000166:cytology*
D002241:Carbohydrates; D002352:Carrier Proteins; D037102:Lectins; D006493:Heparin