Immunobiological activities of synthetic lipid A analogs with low endotoxicity.
Synthetic lipid A analogs, beta(1-6)glucosamine disaccharide 1,4'-bisphosphates, which possesses four tetradecanoyl groups at the 2- and 2'-amino, and 3- and 3'-hydroxyl groups (LA-17-PP), and each two of the (R)-3-hydroxytetradecanoyl and tetradecanoyl groups at the 2- and 2'-amino and 3- and 3'-hydroxyl groups, respectively (LA-18-PP), were far less endotoxic than synthetic (506, LA-15-PP) and bacterial Escherichia coli type lipid A's; neither compound showed any detectable lethal toxicity in chicken embryos or preparatory activity for the local Shwartzman reaction in rabbits. Also both compounds were only weakly pyrogenic and comparably less lethally toxic in galactosamine-loaded mice than the reference synthetic and bacterial lipid A's and a synthetic counterpart to biosynthetic lipid A precursor Ia (406, LA-14-PP). Nevertheless, LA-17-PP and LA-18-PP exhibited definite in vivo immunoadjuvant activity in mice, and the ability to induce a possible tumor necrosis factor and alpha/beta interferon in Mycobacterium bovis BCG and Propionibacterium acnes-primed mice, respectively, although these activities were weaker than those of the reference lipid A's. 4'-Monophosphate analogs of the above two test compounds exhibited neither endotoxic nor beneficial activities, but they showed remarkable in vitro bioactivities comparable to those of the corresponding bisphosphate compounds; the ability to activate the human complement system and the clotting enzyme cascade of horseshoe crab amoebocyte lysate, stimulatory effects on guinea pig and murine peritoneal macrophages, and murine splenocytes.
D000276:Adjuvants, Immunologic; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology* / Q000633:toxicity; D003167:Complement Activation; D004731:Endotoxins / Q000276:immunology* / Q000633:toxicity; D006023:Glycoproteins / Q000096:biosynthesis; D006168:Guinea Pigs; D007370:Interferon Type I / Q000096:biosynthesis; D008033:Limulus Test; D008050:Lipid A / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology / Q000633:toxicity; D008262:Macrophage Activation; D051379:Mice; D008815:Mice, Inbred Strains; D011749:Pyrogens; D012790:Shwartzman Phenomenon; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha
D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D004731:Endotoxins; D006023:Glycoproteins; D007370:Interferon Type I; D008050:Lipid A; D011749:Pyrogens; D014409:Tumor Necrosis Factor-alpha