title: CB 1954 (2,4-dinitro-5-aziridinyl benzamide) becomes a DNA interstrand crosslinking agent in Walker tumour cells.
abstract: Walker tumour cells were shown to be uniquely sensitive to CB 1954 when compared with other cells in vitro. CB 1954 forms DNA-DNA interstrand crosslinks in a time-dependent manner in Walker tumour but not Chinese hamster cells. The absence of interstrand crosslinks in hamster cells was not due to a lack of uptake of drug but rather to a failure to convert (probably by bioreduction) CB 1954 to the required reactive difunctional intermediate.
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D001388:Aziridines / Q000378:metabolism / Q000494:pharmacology*; D001389:Azirines / Q000494:pharmacology*; D002279:Carcinoma 256, Walker / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003432:Cross-Linking Reagents; D004273:DNA, Neoplasm / Q000187:drug effects*; D066298:In Vitro Techniques; D051381:Rats
chemical_list: D000970:Antineoplastic Agents; D001388:Aziridines; D001389:Azirines; D003432:Cross-Linking Reagents; D004273:DNA, Neoplasm; C100099:tretazicar