Evidence for the repetitive domain structure of pig calpastatin as demonstrated by cloning of complementary DNA.
A clone of complementary DNA (cDNA) coding for pig heart calpastatin was isolated using synthetic oligonucleotide probes. The amino acid sequence deduced from the nucleotide sequence revealed the occurrence of a repetitive sequence at the interval of 140 amino acids, substantiating the multidomain structure of calpastatin. A portion of the sequence of 251 amino acid residues predicted for pig heart calpastatin (107 kDa) was found to be identical with that of a peptide fragment derived from pig erythrocyte calpastatin (68 kDa) and sequenced by Edman degradation.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D009206:Myocardium / Q000201:enzymology; D013552:Swine
D002135:Calcium-Binding Proteins; C033668:calpastatin; D004247:DNA