Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells.
We have isolated, cloned and analyzed small polydisperse circular (spc) DNA from mouse 3T6 cells. The representation of highly repeated mouse genome sequence families in spcDNA has been examined, and the B1 repeat appears overrepresented in spcDNA by two criteria. The majority of spcDNA clones, however, is made out by as yet uncharacterized middle repetitive sequences. We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D004270:DNA, Circular / Q000235:genetics* / Q000302:isolation & purification; D005784:Gene Amplification; D051379:Mice / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004270:DNA, Circular; D003513:Cycloheximide