A vector-primer-cloner-sequencer plasmid for the construction of cDNA libraries: evidence for a rat glyceraldehyde-3-phosphate dehydrogenase-like mRNA and a ferritin mRNA within testis.
We have developed a vector-primer-cloner-sequencer (VPCS), based on the pUC plasmids, which is easy to prepare. Stable cDNA libraries have been generated from human, rat, and bull testis. The inserts are of various size classes, including high-molecular-weight reverse transcripts which can be easily sequenced. The utility of this VPCS has been demonstrated by isolating a rat ferritin and glyceraldehyde-3-phosphate dehydrogenase-like clone.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D005293:Ferritins / Q000235:genetics*; D005822:Genetic Vectors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006801:Humans; D008297:Male; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013737:Testis / Q000502:physiology*
D012333:RNA, Messenger; D004247:DNA; D005293:Ferritins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases