Repair of etheno DNA adducts by N-glycosylases.
After incubation of chloroacetaldehyde-treated DNA with cell-free homogenates, the excision of N2,3-ethenoguanine and 1,N6-ethenoadenine was observed with a rat brain tumour cell line. The repair mechanism was that of an N-glycosylase. The high specificity of all known DNA N-glycosylases and some unique properties of the enzymatic reaction indicate the existence of N-glycosylases specific for the repair of etheno, or similar, adducts.
D000079:Acetaldehyde / Q000031:analogs & derivatives; D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D045647:DNA Glycosylases; D004260:DNA Repair; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006151:Guanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity
C015196:N(2),3-ethenoguanine; D006151:Guanosine; C004981:1,N(6)-ethenoadenine; C007640:1,N(6)-ethenoadenosine; D006147:Guanine; C004656:chloroacetaldehyde; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D000079:Acetaldehyde; D000225:Adenine; D000241:Adenosine