[Inhibition of ADP-ribosylation by nerve growth factor in the pheochromocytoma PC-12 cell line].
The effect of nerve growth factor (NGF), purified to homogeneity from bovine seminal plasma using HPLC, on the level of endogenous ADP-ribosylation in pheochromocytoma PC-12 cell line was studied. NGF caused a 30% inhibition of ADP-ribosylation in the cellular homogenate, a 25% inhibition during serum-free cultivation, and a 50% inhibition in the presence of serum in the culture medium. NGF inhibited ADP-ribosylation of several proteins, including a protein with molecular weight of 40,000, probably of membrane origin. A possibility of the interrelation between NGF and cyclase system via receptor-dependent ADP-ribosylation of regulatory components of the adenylate cyclase was discussed.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D002460:Cell Line; D003864:Depression, Chemical; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000378:metabolism*
D009414:Nerve Growth Factors; D000246:Adenosine Diphosphate Ribose
Ingibirovanie ADP-ribozilirovaniia pod deĭstviem faktora rosta nervov v vkletochnoĭ linii feokhromotsitomy PC-12.