Enhancement of noradrenaline release by 12-O-tetradecanoyl phorbol-13-acetate, an activator of protein kinase C.
12-O-Tetradecanoyl phorbol-13-acetate (TPA), an activator of protein kinase C (PKC), enhanced the electrically evoked overflow of [3H]noradrenaline in a concentration-dependent manner in rabbit hippocampal slices. 4-O-Methyl-TPA, which lacks the ability to activate PKC had no effect on the evoked tritium overflow. The enhancement of noradrenaline release by TPA was affected by neither the alpha 2-adrenoceptor antagonist yohimbine nor the alpha 2-adrenoceptor agonist clonidine. It is concluded from these results that PKC is involved in the mechanism of stimulus-secretion coupling in noradrenergic nerve terminals of the rabbit hippocampus.
D000818:Animals; D004558:Electric Stimulation; D004789:Enzyme Activation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D011817:Rabbits; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D009638:Norepinephrine