Effect of testosterone replacement therapy on quantitative spermatogenesis following plumbagin treatment in immature rats.
Plumbagin (2-methyl, 5-hydroxy 1,4 naphthoquinone) was isolated from the root of Plumbago zeylenica L. It caused a decrease in the total number of spermatids, resting and pachytene spermatocytes as well as a significant reduction in seminiferous tubule and Leydig cell nuclei diameter, when given to immature rats at the rate of 1 mg/100 g body wt., orally/day for 32 days. Subsequently testosterone injections were given in low and high doses (0.5 mg and 1 mg/100 g body wt., im/day) on day-26 through day-32 to know whether such changes in quantitative spermatogenesis are produced as a result of the direct effect of plumbagin on the testis or through the inhibition of pituitary gonadotropin release.
D000367:Age Factors; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D008297:Male; D009285:Naphthoquinones / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013076:Sperm Count / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000033:anatomy & histology; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects; D013739:Testosterone / Q000494:pharmacology*
D009285:Naphthoquinones; D013739:Testosterone; C014758:plumbagin