An enzymatic method for the synthesis of [14C]pyridoxal 5'-phosphate from [14C]pyridoxine.
A new enzymatic method for the synthesis of [14C]pyridoxal 5'-phosphate is presented. [14C]Pyridoxal 5'-phosphate was synthesized from [14C]pyridoxine through the successive actions of pyridoxal kinase and pyridoxamine 5'-phosphate oxidase in a reaction mixture containing ATP, [14C]pyridoxine, and both enzymes. [14C]Pyridoxal 5'-phosphate was isolated by omega-aminohexyl-Sepharose 6B column chromatography. The overall yield of the product was more than 60%, starting from 550 nmol of [14C]pyridoxine. The radiochemical purity of the products, as determined by thin-layer and ion-exchange chromatography, was greater than 98%.
D001219:Aspartate Aminotransferases / Q000032:analysis; D002847:Chromatography, Agarose; D066298:In Vitro Techniques; D011731:Pyridoxal Kinase / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000096:biosynthesis* / Q000302:isolation & purification; D011734:Pyridoxaminephosphate Oxidase / Q000378:metabolism; D011736:Pyridoxine / Q000378:metabolism*
D011732:Pyridoxal Phosphate; D011734:Pyridoxaminephosphate Oxidase; D001219:Aspartate Aminotransferases; D011731:Pyridoxal Kinase; D011736:Pyridoxine