The regulation of tissue eosinophilia. V. Induction of lymphocyte-derived eosinophil chemotactic inhibitory factor production by a macrophage product from complete Freund's adjuvant-treated guinea pigs.
Splenic T lymphocytes of complete Freund's adjuvant (CFA)-treated guinea pigs spontaneously produce a lymphokine, eosinophil-directed chemotactic inhibitory factor (ECIF), which selectively inhibits the chemotactic response of the treated eosinophils to a certain chemotactic lymphokine for eosinophils. In the present paper, induction of spontaneous ECIF production was examined in CFA-treated guinea pigs. Although short-time (3-hr) cultured supernatants of CFA-treated spleen cells exhibited little or no ECIF activity, the supernatants could stimulate normal T lymphocytes to produce and release distinct ECIF activity. The factor with ECIF-releasing activity (ECIF-RF) was produced by splenic adherent cells of CFA-treated animals. ECIF-RF activity was absorbed only by T lymphocytes, suggesting that target cells of ECIF are T lymphocytes. ECIF-RF activity was eluted near cytochrome c (MW 12,500) on a Sephadex G-100 column. It was further found that ECIF-RF was sensitive to heating at 56 degrees C, to enzyme treatment with trypsin and neuraminidase, and to acid and alkaline condition. We thus concluded that ECIF-RF derived from adherent cells of CFA-treated animals may be involved in the suppression of cell-mediated tissue eosinophilia by stimulation of T lymphocytes to produce and release ECIF activity.
D000818:Animals; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors*; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis; D002850:Chromatography, Gel; D004802:Eosinophilia / Q000209:etiology*; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006168:Guinea Pigs; D008222:Lymphokines / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D015846:Monokines; D014176:Protein Biosynthesis
D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil; D008222:Lymphokines; D015846:Monokines; C034397:chemotactic inhibitory factor; D005620:Freund's Adjuvant