title: Localization of the LDHA gene to 11p14----11p15 by in situ hybridization of an LDHA cDNA probe to two translocations with breakpoints in 11p13.
abstract: Lymphoblastoid cell lines established from two individuals with apparently balanced translocations involving 11p13 were used for LDHA regional localization. The karyotypes were 46,XY,t(4;11)(q21;p13) and 46,XY,t(1;11)(p22;p13). In situ hybridization of a human LDHA cDNA probe to chromosome preparations from these cell lines resulted in specific labeling over bands p14----p15 of the normal chromosomes 11 and over bands 11p14----11p15 of the derivative chromosomes 4 and 1. These results exclude LDHA from any region proximal to 11p13 and localize the gene to 11p14----11p15.
mesh_terms: D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000235:genetics; D006801:Humans; D007527:Isoenzymes; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014178:Translocation, Genetic
chemical_list: D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase