Proposal of standardized methods and reference for assaying recombinant human tumor necrosis factor.
Two assay methods for recombinant human tumor necrosis factor (rH-TNF) were developed, one a biological L-cell assay and the other an enzyme-linked immunosorbent assay. The accuracy and reproducibility of each and the correlation between the two were studied. As a result of this investigation, the two assay methods were found appropriate for standardization of rH-TNF. A freeze-dried reference was prepared, and examination of its potency and stability showed it to be suitable for use as a reference standard for rH-TNF assays.
D000911:Antibodies, Monoclonal; D001681:Biological Assay; D002470:Cell Survival / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005612:Freeze Drying; D006023:Glycoproteins / Q000276:immunology / Q000494:pharmacology / Q000592:standards*; D006801:Humans; D007739:L Cells; D014409:Tumor Necrosis Factor-alpha
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D014409:Tumor Necrosis Factor-alpha