title: A spontaneously produced lipopolysaccharide biosynthetic defect which causes both pleiotropic phage resistance and mucoid colony morphology in Salmonella anatum.
abstract: A spontaneously derived mutant of the smooth bacterial strain, Salmonella anatum A1, specifically blocks the DNA ejection function of bacteriophage E15 during infection. The mutant, AE15R-5, exhibits mucoid colony morphology but no evidence of colanic acid biosynthesis. It is resistant not only to bacteriophage E15, but also to all other smooth- and rough-specific phages which have been tested. Chemical, immunological and gel electrophoretic analyses indicate that its lipopolysaccharide (LPS) molecules fall into two categories: they are either highly truncated (probably heptoseless) or extremely large (complete LPS molecules with O-polysaccharides containing 80 or more repeat units). The antibiotic resistance pattern of AE15R-5 is roughly intermediate between that of a known heptoseless mutant, S. anatum MG4, and that of the parent strain, S. anatum A-1.
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D004279:DNA, Viral / Q000378:metabolism; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D008070:Lipopolysaccharides / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D009154:Mutation; D009675:Novobiocin / Q000494:pharmacology; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000096:biosynthesis; D012475:Salmonella / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012483:Salmonella Phages / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D009675:Novobiocin; D012967:Sodium Dodecyl Sulfate; D013752:Tetracycline