Decreased feed consumption and body-weight gain in the B6C3F1 mouse after dietary exposure to 15-acetyldeoxynivalenol.
15-Acetyldeoxynivalenol (15-ADON), a biosynthetic precursor of deoxynivalenol (DON), was extracted from rice cultures of Fusarium graminearum R6576 and purified. Growing female B6C3F1 mice were fed semi-purified diets containing 0, 0.5, 2.0 and 5.0 ppm 15-ADON over 56 days and assessed for effects on feed intake, body-weight gain, terminal organ weights and blood clotting function. A significant reduction in feed intake was observed at the 5.0-ppm level after 44 days, whereas reduced rates of weight gain were found to occur at the 5.0-ppm level after only 16 days. Terminal liver, kidney and spleen weights were significantly lower in mice consuming the 5.0-ppm diet when compared with controls. Dietary 15-ADON at the 0.5- and 2.0-ppm levels did not show significant effects on weight gain, feed intake or organ weights. Although mice treated with 15-ADON had significantly decreased bleeding times, other measurements of clotting function indicated no differences between the control and treated groups. Results indicated that 15-ADON was only slightly less toxic than DON and that chronic manifestations of dietary 15-ADON were similar to those found previously for DON. Future risk assessments for DON should therefore include consideration of 15-ADON occurrence and toxicity.
D000818:Animals; D001760:Bleeding Time; D001777:Blood Coagulation / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects*; D004435:Eating / Q000187:drug effects*; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D012717:Sesquiterpenes / Q000494:pharmacology*; D014255:Trichothecenes / Q000494:pharmacology*
D012717:Sesquiterpenes; D014255:Trichothecenes; C046760:15-acetyldeoxynivalenol