Comparative binding of wheat germ agglutinin and its succinylated form on lymphocytes.
We have compared the binding parameters of native wheat germ agglutinin (WGA) and its succinylated form (SWGA) to rat lymphocytes. Scatchard plots were obtained with the fluoresceinated lectins in a concentration range of 10 nM to 0.1 mM. Association and dissociation rate parameters were also measured. The following differences were observed: at low concentration of WGA, binding is positively cooperative with a Hill coefficient of 1.75, whereas binding of SWGA is not. The numbers of high-affinity sites are respectively (2.5 +/- 0.8) X 10(6) and (6.4 +/- 1.3) X 10(5) for WGA and SWGA. Association constants were found to be (4.7 +/- 1.7) X 10(6) l mol-1 for WGA and (1.42 +/- 0.36) X 10(7) l mol-1 for SWGA, which is 35 times higher than for native WGA. Neuraminidase treatment decreases the Hill coefficient as well as the number of sites involved in the cooperative binding of native WGA. Equilibrium data were obtained at three temperatures to determine the thermodynamic parameters (delta H degree and delta S degree). These results are indicative of an oligomerization process dynamically formed at the membrane level before tight binding of the lectin to its receptors could occur.
D000818:Animals; D001665:Binding Sites; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008433:Mathematics; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000378:metabolism; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
D014909:Wheat Germ Agglutinins; C030717:succinylated wheat germ agglutinin