Measurement of liver adenine nucleotides and S-adenosyl amino acids by one-step high-performance liquid chromatography.
A reverse-phase isocratic HPLC method is described for direct simultaneous assay of ATP, ADP, AMP, S-adenosylmethionine, S-adenosylhomocysteine, S-adenosylethionine, and other adenine derivatives in liver microbiopsies. The procedure was tested in conditions which alter the hepatic content of adenine nucleotides and sulfur-adenosyl amino acids in humans, rats, and guinea pigs.
D000227:Adenine Nucleotides / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis; D000603:Amino Acids, Sulfur / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006168:Guinea Pigs; D006801:Humans; D008099:Liver / Q000032:analysis*; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000032:analysis; D012436:S-Adenosylmethionine / Q000032:analysis
D000227:Adenine Nucleotides; D000603:Amino Acids, Sulfur; D012436:S-Adenosylmethionine; D000255:Adenosine Triphosphate; D012435:S-Adenosylhomocysteine