title: Demonstration by immunoblotting of heterogeneity in the autoantibody response directed against fat cells in Graves' disease.
abstract: Guinea pig fat cell membranes (FCM) have been widely used in preference to thyroid membranes as a source of TSH receptors to investigate TSH receptor antibodies in Graves' disease, because FCM are ostensibly free of other thyroid antigens. However, by FCM immunoblotting we have found: 8 of 10 normal sera bound to determinants at 38 and 190 kDa; 17 other determinants were recognised by 60% of Graves' or Hashimoto sera and by 20% of normal sera; three determinants at 65-90 kDa were recognised by 5 of 13 Graves' but by none of the normal or Hashimoto sera; and none of the determinants recognised appeared to be related to the TSH receptor.
mesh_terms: D000273:Adipose Tissue / Q000276:immunology*; D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006111:Graves Disease / Q000276:immunology*; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D011989:Receptors, Thyrotropin / Q000187:drug effects / Q000276:immunology*; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D001323:Autoantibodies; D006063:Chorionic Gonadotropin; D011989:Receptors, Thyrotropin; D013972:Thyrotropin