[Protein mapping of normal circulating cells: lymphocytes and monocytes].
Purified lymphocyte and monocyte populations were obtained from peripheral blood cells by ficoll-hypaque gradient and elutriation in different normal individuals. The two types of mononuclear cells were then analyzed by 2-dimensional gel electrophoresis. More than 1,000 spots were detected by our computer system and analyzed for each cell type. Automatic picture comparison showed a typical pattern for the two different populations with excellent reproducibility.
D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D008214:Lymphocytes / Q000032:analysis*; D009000:Monocytes / Q000032:analysis*
D001798:Blood Proteins
Cartographies protéiques de cellules circulantes normales: lymphocytes et monocytes.