Isolation and cryopreservation of human peripheral blood monocytes.
A modification of the Freundlich and Avdalovic method (J. Immunol. Methods 62, 31 (1983] is reported. Buffy coats, separated and pooled together, are used for isolation of monocytes (70% yield, 100% purity). Cell density of working suspension is increased up to 0.65 X 10(9) cells/75 cm2 surface by multiplication of the active fibronectin sites. For the purpose, cryoprecipitate is used instead of plasma for coating the glass-gelatin surface. Monocytes, isolated by that procedure, could be successfully cryopreserved with dimethyl sulfoxide cryoprotective solution.
D001793:Blood Preservation; D002448:Cell Adhesion; D002470:Cell Survival; D005615:Freezing; D006801:Humans; D009000:Monocytes / Q000166:cytology* / Q000502:physiology; D010587:Phagocytosis; D013997:Time Factors