Detection of UV purine photoproducts in a defined sequence of human DNA.
The UV-irradiated, 3'-end-labeled, 92-base-pair terminus of the human alphoid sequence was incubated with purified endonuclease v. Previously unreported photoproducts were incised at purine loci. These were not pyrimidine photodimers, 6-4'-(pyrimidin-2'-one)-pyrimidines, base loss sites, or ring-opened purines. Therefore, purine-containing photoproducts, possibly dimers, were incised by the enzyme preparation.
D001483:Base Sequence; D002872:Chromosome Deletion; D004247:DNA / Q000528:radiation effects*; D006358:Hot Temperature; D006801:Humans; D010957:Plasmids; D011687:Purines / Q000528:radiation effects*; D014466:Ultraviolet Rays
D011687:Purines; D004247:DNA