Role of granulation tissue and fibroblasts in gingival testosterone metabolism in the rat in vitro.
The metabolism of testosterone by experimental granulation tissue, fibroblasts and the oral mucosa of rats of both sexes was studied. The experimental granulation tissue was produced by implanting viscose-cellulose sponges beneath the dorsal skin of female and male rats for 21 days. The granuloma capsules, fibroblasts in the sponges and the oral mucosae were homogenated. Mitochondrial, microsomal and soluble fractions were incubated with [4-14C]testosterone and NADPH for 30 min at pH 7.4 and 37 degrees C. The metabolites were identified with column and TLC and radioautography and quantified with liquid scintillation counting. The experimental granulation tissue and fibroblasts of both sexes showed less activity in metabolizing testosterone than the gingival tissue. The tissues were shown to contain 3 alpha-, 3 beta- and 17 beta-hydroxysteroid dehydrogenase and 5 alpha- and 5 beta-steroid hydrogenase activities. The activities of the enzymes in the oral mucosae were higher than in the experimental granulation tissue and fibroblasts.
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D005881:Gingiva / Q000378:metabolism*; D006097:Granulation Tissue / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009061:Mouth Mucosa / Q000378:metabolism; D051381:Rats; D013739:Testosterone / Q000378:metabolism*
D013739:Testosterone