Aromatic DNA adducts in human bone marrow and peripheral blood leukocytes.
DNA from normal human bone marrow mononuclear and non-mononuclear cells was analysed by 32P-post-labelling for the presence of aromatic adducts. Ten out of 10 individuals showed the presence of adducts at levels of 1-9 adducts per 10(8) nucleotides that were not detected in four samples of human foetal bone marrow. Inter-individual variations in the patterns of these presumed aromatic adducts were observed. Similar adducts were also present in the DNA of peripheral white blood cells of both smokers and non-smokers, although at lower levels than in bone marrow. The data suggest that the adducts result from environmental exposure to an as-yet-unidentified genotoxic agent or agents.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D002274:Carcinogens, Environmental / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds / Q000378:metabolism*; D051381:Rats
D002274:Carcinogens, Environmental; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds; D000255:Adenosine Triphosphate; D004247:DNA