[Cholesterol, phospholipid and apoB composition of LDL: comparison of precipitation and ultracentrifugation methods].
Cholesterol, phospholipid and apoB levels were determined in LDL precipitated by amphiphatic polymers (Biomérieux kit, Marcy-l'Etoile, France). Intra-assay and inter-assay analysis performed on 4 serum pools were satisfactory. For 113 sera (triglyceride level less than 4.5 mmol/l, absence of VLDL-remnants), the 3 variables were well correlated with those of centrifuged LDL (0.93 less than r less than 0.98); however, LDL-cholesterol values were significantly higher (p less than 0.001), and those of LDL-phospholipids (p less than 0.001) and LDL-apoB (p less than 0.005) significantly lower. The correlations between the 3 constituents studied gave statistically different regression coefficients for the two methods. It is concluded that these precipitated lipoproteins do not seem to correspond to LDL but to "LDL markers".
D001055:Apolipoproteins B / Q000097:blood*; D011232:Chemical Precipitation; D002784:Cholesterol / Q000097:blood*; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood* / Q000302:isolation & purification; D010743:Phospholipids / Q000097:blood*; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation / Q000379:methods
D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol
Composition en cholestérol, phospholipides et apoB des LDL. Comparaison des méthodes par précipitation et ultracentrifugation.