[Intravital acellular hemolysis of extravascular erythrocytes. An experimental contribution to the determination of wound age].
The time-dependent acellular, destructive changes in erythrocytes was investigated in rabbits by subcutaneous, intraperitoneal, and subdural implantation of blood-filled diffusion chambers with 0.45 micron pores. The animals were killed at various intervals after implantation (7-24 h), and the erythrocytes in the capsules were examined by light and electron microscopy. The findings were quantified by counting 100 cells per preparation under the light microscope; changes in both matrix and membrane structure were recorded. Matrix changes and membrane variants were specified by electron microscopy. Time-dependent changes in erythrocyte matrix and membrane were established, which occurred at the various localizations in an identical sequence, but with different time intervals. The destructive changes were: an increase in loss of matrix, increase in membrane permeability, and decrease in membrane stability. The physiologic bases of and the conclusions for wound timing were discussed.
D000818:Animals; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D004912:Erythrocytes / Q000473:pathology*; D005260:Female; D006461:Hemolysis; D006470:Hemorrhage / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D011817:Rabbits; D014947:Wounds and Injuries / Q000473:pathology*
Intravitale, azelluläre Hämolyse extravasaler Erythrozyten. Ein experimenteller Beitrag zur Wundaltersbestimmung.