Structures of the sugar chains of a major glycoprotein present in the egg jelly coat of a starfish, Asterias amurensis.
Sugar chains of a major glycoprotein, obtained from the egg jelly coat of a starfish (Asterias amurensis), were released quantitatively as oligosaccharides by hydrazinolysis. After N-acetylation, they were converted to radioactive oligosaccharides by reduction with NaB3H4. Analysis by paper electrophoresis revealed that all of them were neutral oligosaccharides. Upon Bio-Gel P-4 column chromatography, the radioactive oligosaccharide mixture was separated into four components. Structural study of each component by sequential glycosidase digestion in combination with 1H-NMR spectroscopy revealed that the glycoprotein contains the following oligosaccharides, in which R represents either proton, Glc alpha 1----, Glc alpha 1----3Glc alpha 1----, or Glc alpha 1----2Glc alpha 1----3Glc alpha 1----. (Formula: see text)
D000107:Acetylation; D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D001894:Borohydrides; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002845:Chromatography; D004590:Electrophoresis, Paper; D006023:Glycoproteins / Q000032:analysis*; D006026:Glycoside Hydrolases; D006834:Hydrazines; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis; D010063:Ovum / Q000032:analysis*; D013215:Starfish / Q000032:analysis*
D001894:Borohydrides; D006023:Glycoproteins; D006834:Hydrazines; D009844:Oligosaccharides; C025364:sodium borohydride; D006026:Glycoside Hydrolases; D008358:Mannose; D000117:Acetylglucosamine