[DNA synthesis in a stationary HeLa cell culture following gamma irradiation].
Residual incorporation of 3H-thymidine into acid insoluble fraction was inhibited a few hours and stimulated 24 hours following gamma-irradiation (60Co) of a stationary culture of HeLa cells with doses of 5 to 50 Gy. The dose-response curve for the stimulated incorporation reached a maximum at a dose of about 10 Gy. Hydroxyurea (10 mM) was shown to suppress the incorporation. The authors suggest that ionizing radiation induces a transfer of resting cells to the S phase-like state.
D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D004261:DNA Replication / Q000187:drug effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006367:HeLa Cells / Q000528:radiation effects*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea
Sintez DNK v statsionarnoĭ kul'ture kletok HeLa posle gamma-oblucheniia.