Concanavalin A-induced agglutination of Ehrlich ascites carcinoma cells during growth of the tumor in mice.
ConA-induced agglutination of Ehrlich ascites carcinoma (EAC) cells during development of the tumor in mice has been studied in vitro. EAC cells were incubated with different concentrations of ConA at 37 degrees C for various intervals of time and the cell aggregates of different sizes were scored under microscope. The older EAC cells (20-day-old) showed a decrease in cytoagglutination with respect to large aggregates formation compared to the younger (10-day-old) ones. The small aggregates formed by the 5-day-old EAC cells are dissociated by competition with alpha-methyl-D-mannoside but the large aggregates are not affected by the sugar treatment. The observed differences in the agglutination patterns are discussed in view of the influence of the lipid content of EAC cells and the ascites fluid on ConA-induced cytoagglutination.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D002449:Cell Aggregation / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D050356:Lipid Metabolism; D008766:Methylmannosides / Q000494:pharmacology; D051379:Mice
D008766:Methylmannosides; D003208:Concanavalin A; C008466:methylmannoside