title: Investigations into negative interference by jaundiced plasma in kinetic Jaffé methods for plasma creatinine determination.
abstract: Negative interference by jaundiced plasma in kinetic Jaffé methods for plasma creatinine determination is caused by both conjugated and unconjugated bilirubin. The amount of interference observed is method- and temperature-dependent and is directly related to bilirubin concentration but is independent of the creatinine concentration. Bilirubin also significantly reduces the precision of the creatinine estimation. A mechanism of bilirubin interference in kinetic Jaffé methods is proposed and the influence of picrate concentration, hydroxide concentration and temperature on the magnitude of bilirubin interference has been investigated.
mesh_terms: D001663:Bilirubin / Q000097:blood; D003404:Creatinine / Q000097:blood*; D005188:False Negative Reactions; D006801:Humans; D006878:Hydroxides; D007565:Jaundice / Q000097:blood*; D007700:Kinetics; D010851:Picrates; D013696:Temperature
chemical_list: D006878:Hydroxides; D010851:Picrates; D003404:Creatinine; D001663:Bilirubin