title: Differential repair of gamma-ray-induced DNA strand breaks by various cellular subpopulations of mouse jejunal epithelium and bone marrow in vivo.
abstract: We have examined the induction and repair of gamma-ray-induced DNA strand breaks in different subpopulations of cells in mouse jejunal epithelium and bone marrow using a modification of the alkaline elution methodology whereby different populations of cells are selectively labeled with radioactive DNA precursors. Mice were labeled by intraperitoneal injection with between 0.5 and 2.0 mu Ci/g of [3H]thymidine at various times prior to irradiation with 10 Gy of gamma rays. In the studies with jejunal epithelium, the timing of the injection of the radiolabel relative to the irradiation was varied between 6 and 72 h, depending on the cell population of interest. The DNA damage and repair characteristics representative of both the total cell population and the radiolabeled fraction of these cells were then measured. Little difference was noted in the amount of initial damage induced in these different populations of cells. However, for both the jejunum and bone marrow, cells that incorporated the radiolabel within 6 h after injection (i.e., rapidly proliferating cells) repaired their strand breaks more rapidly than did the remainder of the population. In the case of jejunum, the repair capacity of the radiolabeled cell population progressively diminished as the cells matured and differentiated so that cells that contained the radiolabel 72 h after injection (i.e., mature villus cells) actually repaired their strand breaks more slowly than did the bulk cells.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology* / Q000528:radiation effects; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology / Q000528:radiation effects; D005720:Gamma Rays; D007413:Intestinal Mucosa / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D007583:Jejunum / Q000166:cytology / Q000502:physiology / Q000528:radiation effects; D007700:Kinetics; D051379:Mice