Nucleotide sequence of a cDNA coding for mitochondrial fumarase from human liver.
The nucleotide sequence of a 1.46 kb cDNA, selected from a human liver library by the expression of fumarase antigenic determinants, was determined using the dideoxy chain termination method. The cDNA contained an open reading frame extending from the extreme 5'-base and coding for a protein with 468 amino acids. This protein, with the exception of an N-terminal methionine, was identified as mitochondrial fumarase. The protein showed a high degree of identity of structure with the fumarase from Bacillus subtilis (56.6%) and a fumarase from Escherichia coli (product of the fumC gene, 59.3%), and a lower degree of identity with the aspartase of E. coli (37.2%).
D000595:Amino Acid Sequence; D001220:Aspartate Ammonia-Lyase / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005649:Fumarate Hydratase / Q000235:genetics*; D006801:Humans; D008930:Mitochondria, Liver / Q000201:enzymology
D004247:DNA; D005649:Fumarate Hydratase; D001220:Aspartate Ammonia-Lyase