Bioluminescent assays using coimmobilized enzymes.
In summary the use of immobilized luciferases along with other enzymes offers a method for measuring a wide variety of metabolites or enzymes. The assays are rapid, sensitive, and specific and can be automated. It is anticipated that many more assays for different compounds will be developed in the future.
D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D008156:Luciferases / Q000378:metabolism*; D008163:Luminescent Measurements; D010088:Oxidoreductases / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods; D014733:Vibrio / Q000201:enzymology
D004800:Enzymes, Immobilized; D010088:Oxidoreductases; D008156:Luciferases; D008058:Dihydrolipoamide Dehydrogenase