title: Increased clearance rate of prednisone in the isolated perfused liver of uremic rats.
abstract: The effect of uremia upon the hepatic clearance rate of prednisone was studied in isolated perfused livers of normal and uremic female rats. The perfusion rate was kept at a constant flow rate of 25 ml/min. In order to induce uremia, one group of rats was 5/6 nephrectomized 3 weeks prior to the perfusions. The concentrations of prednisone and other steroids were measured by a normal phase HPLC technique. In experiments with an initial prednisone concentration of 500-700 micrograms/l, the hepatic clearance rate of prednisone was significantly (p less than 0.001) increased by 40% in livers obtained from uremic rats (11.1 +/- 0.49 ml/min/liver) as compared to control rats (8.0 +/- 1.17 ml/min/liver). No significant correlation was found between the hepatic clearance rate of prednisone and increasing initial prednisone concentrations from 141 to 1,953 micrograms/l in the perfusate. It is concluded that an adaptive mechanism may exist in uremia which results in an increase of the hepatic clearance rate of prednisone.
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004305:Dose-Response Relationship, Drug; D005260:Female; D005943:Gluconeogenesis; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D010101:Oxygen Consumption; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D011241:Prednisone / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014511:Uremia / Q000378:metabolism*
chemical_list: D011241:Prednisone