OF4949, new inhibitors of aminopeptidase B. I. Taxonomy, fermentation, isolation and characterization.
New aminopeptidase B inhibitors that we named OF4949-I, II, III and IV were isolated from the culture broth of a fungus, Penicillium rugulosum OF4949. The molecular formula of I was C23H26N4O8 and that of II, C22H24O8, judging from elemental analysis and secondary ion mass spectrometry. The concentrations of I, II, III and IV required for 50% inhibition of aminopeptidase, using Ehrlich ascites carcinoma cells as the source of the enzyme, were 0.0054, 0.0048, 3.4 and 1.7 micrograms/ml, respectively. Components I and II augmented delayed-type hypersensitivity in mice to sheep red blood cells.
D000626:Aminopeptidases / Q000037:antagonists & inhibitors*; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005285:Fermentation; D051379:Mice; D008815:Mice, Inbred Strains; D010407:Penicillium / Q000145:classification / Q000378:metabolism; D010456:Peptides, Cyclic / Q000302:isolation & purification* / Q000494:pharmacology
D010456:Peptides, Cyclic; C051499:OF 4949-IV; C051498:OF 4949-III; C051497:OF 4949-II; C051496:OF 4949-I; C051701:OF 4949-F; C051700:OF 4949-D; D000626:Aminopeptidases; C019721:aminopeptidase B