title: Histone H2B repression causes cell-cycle-specific arrest in yeast: effects on chromosomal segregation, replication, and transcription.
abstract: To determine which cellular processes are dependent on histones, we blocked histone H2B mRNA synthesis in asynchronously growing yeast after fusing the H2B gene to a repressible GAL10 promoter. Chromosomal segregation, replication, and transcription were then examined. We found that the cells arrested in mitosis, with a cell division cycle (cdc) phenotype. Chromatin structure and nuclear segregation were disrupted. A full round of DNA replication took place after the repression of histone H2B mRNA synthesis. Active transcription and the induction of new transcripts also continued in the arrested cells.
mesh_terms: D002453:Cell Cycle / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D004271:DNA, Fungal / Q000096:biosynthesis; D006657:Histones / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D015003:Yeasts / Q000166:cytology / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D006657:Histones; D012331:RNA, Fungal; D012333:RNA, Messenger