title: Higher order folding of chromatin is induced in different ways by monovalent and by bivalent cations.
abstract: The condensation of the 10 nm chromatin filament in the 30 nm fiber by monovalent cations, polyamines and bivalent cations was studied with light scattering at 90 degrees and flow linear dichroism methods. It was found that monovalent cation- and polyamine-induced folding was a two-step process: a precondensation, when a rotation of nucleosomes takes place only, and a condensation step without changes in nucleosome orientation. Divalent cations affected the structure of chromatin in one step only -- condensation of the chromatin filament being accompanied by nucleosome reorientation.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002413:Cations, Divalent; D002417:Cattle; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013950:Thymus Gland
chemical_list: D002413:Cations, Divalent; D002843:Chromatin; D013096:Spermine; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium; D013095:Spermidine