An antibody-desferrioxamine conjugate labelled with 67Ga.
The optimum conditions for producing a 67Ga labelled antibody-desferrioxamine conjugate were determined. The mouse monoclonal antibody LICR-LON-M8 was coupled to the metal chelating compound desferrioxamine (DFO) using glutaraldehyde (GLUT). A DFO:GLUT:M8 molar ratio of 500:150:1 gave an immunoreactive antibody with low amounts (less than 5%) of high molecular weight polymer. The labelling efficiency with 67 Ga was greater than 90%, with a specific activity of 2-4 MBq/mg antibody. This 67Ga labelled antibody is suitable for evaluation as a diagnostic imaging agent.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D003676:Deferoxamine; D005260:Female; D005710:Gallium Radioisotopes; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D018396:Mucin-1; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D005710:Gallium Radioisotopes; D008565:Membrane Proteins; D018396:Mucin-1; D003676:Deferoxamine