title: Correlation between adherence of Erysipelothrix rhusiopathiae strains of serovar 1a to tissue culture cells originated from porcine kidney and their pathogenicity in mice and swine.
abstract: Adherence of four virulent and four avirulent strains of Erysipelothrix rhusiopathiae, serovar 1a, to porcine kidney cell lines, PK-15 and ESK cells, was examined in an in vitro system. The virulent strains adhered well to the cells (range of means, 9.95 +/- 0.87-36.01 +/- 1.10 per cell). In contrast, the avirulent strains showed negligible adherence to the cells (range of means, 0.11 +/- 0.04-1.41 +/- 0.13 per cell). Pretreatment of bacteria with heat, trypsin, or antiserum resulted in a marked decrease in adherence. Scanning electron microscopic examination revealed that the bacteria attached directly to the microvilli of cells.
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D004888:Erysipelothrix / Q000378:metabolism / Q000472:pathogenicity* / Q000648:ultrastructure; D005260:Female; D007668:Kidney; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000382:microbiology*; D013552:Swine; D013554:Swine Erysipelas / Q000382:microbiology; D014774:Virulence