Cholesterol esterification in mouse peritoneal macrophages in the presence of pathological human plasma lipoproteins.
Mouse peritoneal macrophages were incubated with abnormal lipoproteins (LP-X, HDL-E, VLDL-p, IDl-p and LDL-p) from a patient with secondary deficiency in phosphatidylcholine-sterol acyltransferase, or with phosphatidylcholine/cholesterol liposomes, and the stimulation of cholesteryl ester formation was studied. Acetylated low density lipoproteins served as a control. It was found that macrophages incubated with LP-X, the other pathological lipoproteins or with liposomes did not show an enhanced cholesterol esterification. Also HDL-E had no effect despite of its high apoE content and the fact that apoE has been postulated to be the agonist in beta-VLDL binding to macrophages.
D000818:Animals; D002788:Cholesterol Esters / Q000096:biosynthesis*; D005260:Female; D066298:In Vitro Techniques; D008073:Lipoprotein-X / Q000097:blood / Q000494:pharmacology; D008074:Lipoproteins / Q000097:blood / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000097:blood / Q000494:pharmacology; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice
D002788:Cholesterol Esters; D008073:Lipoprotein-X; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008081:Liposomes