[Characteristics of histamine-sensitive adenyl cyclase from cells of the gastric mucosa of the rat].
Effects exerted by specific modifiers and ions on the adenylate cyclase activity of coarse membrane fractions of cells isolated from the rat gastric mucosa, were investigated. Fluoride ions cause 7-8-fold activation of the enzyme affecting neither the enzyme's affinity towards Mg2+-ATP, nor the cooperativeness of substrate binding. Cations Mg2+ and Mn2+ produce activating while Ca2+ inhibiting effects. GTP (10-5 M) enhances histamine activation of the enzyme and reduces maximal stimulating concentrations of histamine. GTP and Gpp(NH)p exert activating action on the enzyme causing 2- and 5-fold activation, resp. Adenosine suppresses the enzyme activity, the effect being potentiated by GTP. The plausible role of N-protein in the regulation of the enzyme activity is discussed.
D000241:Adenosine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D005753:Gastric Mucosa / Q000201:enzymology*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D051381:Rats
D006165:Guanylyl Imidodiphosphate; D008345:Manganese; D006632:Histamine; D006160:Guanosine Triphosphate; D000262:Adenylyl Cyclases; D008274:Magnesium; D000241:Adenosine; D005459:Fluorides; D002118:Calcium
Kharakteristika gistamin-chuvstvitel'noĭ adenilattsiklazy iz kletok slizistoĭ obolochki zheludka krysy.