Conversion of radiolabelled ethanolamine plasmalogen into the dimethylethanolamine and choline analogue via transphosphatidylation by phospholipase D from cabbage.
Starting from biosynthetically prepared ethanolamine plasmalogen 14C-labelled in the O-alkenyl moiety, choline and dimethylethanolamine plasmalogen were prepared by transphosphatidylation utilizing phospholipase D from cabbage. Investigation of the time course of the reaction showed that transphosphatidylation was simultaneously accompanied by hydrolysis of both the substrate and the desired product, resulting in a maximum of product yield after 1-3 h under the reaction conditions investigated. Optimal reaction conditions gave yields of 40% and 62% (of total radioactivity) respectively for the purified choline and dimethylethanolamine derivatives.
D000818:Animals; D001937:Brassica / Q000201:enzymology*; D002250:Carbon Radioisotopes; D007700:Kinetics; D007893:Leishmania donovani / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010955:Plasmalogens / Q000096:biosynthesis* / Q000378:metabolism*
D002250:Carbon Radioisotopes; D010955:Plasmalogens; C032459:choline plasmalogens; C020791:phosphatidal ethanolamines; D010740:Phospholipases; D010739:Phospholipase D