Multiple influences of a heparin-binding growth factor on neuronal development.
Heparin-binding growth factor-2 (HBGF-2; also known as basic fibroblast growth factor) is mitogenic for most anchorage-dependent cells. It is shown here that HBGF-2 stimulates cell-substratum adhesion and neurite extension in the sympathetic nerve cell line PC12. When HBGF-2 is adsorbed to artificial extracellular matrices consisting of heparin or chondroitin sulfate, it causes the formation of cellular aggregates or circles of cells, respectively. HBGF-2 is also a nerve cell survival molecule, for it potentiates the survival of primary cultures of embryonic chick ciliary ganglion cells but not of embryonic neural retina cells. Finally, a series of synthetic peptides from the HBGF-2 sequence is described that selectively alter the biological effects of HBGF-2. The amphiphilic nature of one of these peptides is discussed with respect to its ability to stimulate cell adhesion.
D000310:Adrenal Gland Neoplasms; D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D016222:Fibroblast Growth Factor 2; D006133:Growth Substances / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D007700:Kinetics; D008934:Mitogens / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology*; D010446:Peptide Fragments / Q000138:chemical synthesis; D010673:Pheochromocytoma; D051381:Rats
D006133:Growth Substances; D008934:Mitogens; D010446:Peptide Fragments; D016222:Fibroblast Growth Factor 2; D006493:Heparin