[A rapid method for the determination of cyclosporin A in blood by high performance liquid chromatography].
The purpose of this paper is to propose a method for the determination of cyclosporin in whole blood by means of HPLC with UV detection. After a one step extraction into ether, the organic layer is washed NaOH and evaporated to dryness. The residue is reconstituted in a mixture of acetonitrile and water (40/60) (V/V) and injected into the chromatograph. The C8 reversed phase column is warming at 75 degrees C temperature. The mobile phase consists of acetonitrile and water (60/40) (V/V) and the wavelength is set at 210 nm. The technique is rapid, sensitive, reproducible and well suited for therapeutic monitoring and for pharmacokinetic studies.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003524:Cyclosporins / Q000097:blood*; D006801:Humans
D003524:Cyclosporins
Méthode rapide de dosage de la ciclosporine A dans le sang par chromatographie liquide haute performance.