[Primary cultures of various differentiated human cells and their transfer (2). The isolation of target cells from their specimen].
The specimen employed for the culture should be possibly rich of target cells in order to obtain the successful primary culture. When the target cells are located along the inner side of a duct forming tissue, they can be relatively easily isolated as major cell population in their whole dissegregated cells by means digesting the inner wall of ductive tissues. When the specimen supplied for the culture is so small and limited, the explant outgrowth culture method can be better applied for the culture. For the primary cultures of malignant cells, soft agar cell suspension method can be better available for the successful culture.
D000362:Agar; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D009369:Neoplasms / Q000473:pathology; D013048:Specimen Handling
D003470:Culture Media; D000362:Agar