An efficient method for the introduction of viral DNA into Brevibacterium lactofermentum protoplasts.
A method for the introduction of a bacteriophage DNA into Brevibacterium lactofermentum protoplasts is described. Frequencies of 10(5) infective centres per micrograms DNA were easily achieved, the relationship between the number of infective centres and the amount of DNA being linear up to 5 micrograms DNA per assay. This method can be used to introduce foreign DNA into these bacteria.
D001435:Bacteriophages / Q000235:genetics*; D001951:Brevibacterium / Q000235:genetics* / Q000254:growth & development; D004279:DNA, Viral / Q000235:genetics*; D011523:Protoplasts / Q000502:physiology; D014170:Transformation, Genetic
D004279:DNA, Viral