Solubilization of cell-membrane-antigen and its coupling to fixed and non-fixed erythrocytes.
Human type-A erythrocytes were lysed and solubilized with 0.3% sodium deoxy cholate (DOC). The solubilized membranes were coated onto human type-O glutaraldehyde fixed erythrocytes. The coated erythrocytes were found to agglutinate specifically by anti-A antibodies. Furthermore, solubilized membranes could be successfully coated to non-fixed erythrocytes after dialysing out the DOC. Quantitative absorption assays showed that the density of type-A blood group antigen on the coated erythrocytes was higher than the density of this antigen on human type-A erythrocytes. Erythrocytes that are coated with cell membrane antigens can be used to assess specific antibody titres and also can be applied for a sensitive selection and separation of rosetting lymphocytes that secrete specific antibodies against these antigens.
D000017:ABO Blood-Group System; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D008722:Methods; D012995:Solubility
D000017:ABO Blood-Group System; D000906:Antibodies; D000941:Antigens