Determination of ochratoxin A in animal feed and cereal grains by liquid chromatography with fluorescence detection.
A liquid chromatographic (LC) method is described for determination of ochratoxin A in animal feeds and cereal grains. Samples are initially extracted with chloroform-ethanol (8 + 2) and 5% acetic acid in water. Extracts are purified using a silica gel cartridge followed by a cyano cartridge. The samples are evaporated, diluted to a known volume, and analyzed using a 10 cm column of 3 micron C18 and a fluorescence detector. The method was applied to a variety of animal feeds and cereal grains at levels of 1.0-0.005 ppm added ochratoxin A. The overall recovery was 90.6% +/- 3.6.
D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid; D002523:Edible Grain / Q000032:analysis*; D005506:Food Contamination; D005516:Food Microbiology; D009793:Ochratoxins / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D009793:Ochratoxins; C025589:ochratoxin A