Stability of membrane pores in hypotonically dialyzed erythrocytes: coencapsulation of different stokes radius probes can occur for at least 21 days in human erythrocytes.
Hypotonic dialysis of human erythrocytes results in porous cell stability for several days. Hypotonic cells stored for 1 week are essentially normal with respect to the preparation of carrier erythrocytes. Afterward, cells begin to irreversibly hemolyze resulting in decreased cell recoveries and decreased encapsulation percentages of two probes, sucrose and inulin. The holes generated by controlled hypotonic dialysis (100 mOsm/kg) are unlike the single rupture hole generated by dialysis to 10-20 mOsm/kg. The minimum pore size of resealed, annealed carrier cells is confirmed to be less than 5.2 A.
D001793:Blood Preservation; D002463:Cell Membrane Permeability; D002470:Cell Survival; D003956:Dialysis; D004910:Erythrocyte Membrane / Q000502:physiology / Q000648:ultrastructure*; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D007038:Hypotonic Solutions; D007444:Inulin / Q000097:blood; D008855:Microscopy, Electron, Scanning; D013395:Sucrose / Q000097:blood
D007038:Hypotonic Solutions; D013395:Sucrose; D007444:Inulin