Bryodin, a ribosome-inactivating protein from the roots of Bryonia dioica L. (white bryony).
Bryodin is a strongly basic (pI greater than or equal to 9.5) glycoprotein (neutral sugar content 6.3%) with Mr 30,000, purified from the roots of Bryonia dioica (white bryony). This protein inhibits protein synthesis by a rabbit reticulocyte lysate with and ID50 (concentration causing 50% inhibition) of 0.12 nM (3.6 ng/ml) and has much less effect on protein synthesis by whole cells, with ID50 values ranging from 46 nM to 2.27 microM (1.4-67 micrograms/ml). Bryodin acts by inactivating ribosomes, with a less-than-equimolar ratio, which suggests a catalytic action. Bryodin decreases the number of local lesions induced by tobacco mosaic virus in the leaves of Nicotiana glutinosa. From all its properties, bryodin can be considered to be a ribosome-inactivating protein, similar to those already known [reviews: Barbieri & Stirpe (1982) Cancer Surveys 1, 489-520; Stirpe & Barbieri (1986) FEBS Lett. 195, 1-8].
D004247:DNA / Q000096:biosynthesis; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D046911:Macromolecular Substances; D010649:Phenylalanine / Q000378:metabolism; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D010944:Plants / Q000032:analysis*; D011072:Poly U / Q000494:pharmacology; D014176:Protein Biosynthesis; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014118:Toxins, Biological
D046911:Macromolecular Substances; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D014118:Toxins, Biological; C051829:bryodin; D011072:Poly U; D010649:Phenylalanine; D004247:DNA