Identification of the phosphate-translocator from maize mesophyll chloroplasts.
Intact maize mesophyll chloroplasts have been isolated in yields of up to 10 mg of chlorophyll per preparation. The chloroplasts were able to reduce 3-phosphoglycerate at a rate of 2.4 mumol of oxygen/min/mg of chlorophyll. This activity was inhibited by preincubating the intact chloroplasts with pyridoxal 5-phosphate. Chloroplast envelopes have been prepared and the protein profile has been obtained on SDS-polyacrylamide gels. The phosphate-translocator from the chloroplast envelope has been identified as a 30kDa polypeptide.
D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D005988:Glyceric Acids / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D010084:Oxidation-Reduction; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D003313:Zea mays / Q000378:metabolism
D002352:Carrier Proteins; D005988:Glyceric Acids; D028044:Phosphate-Binding Proteins; D010710:Phosphates; D011732:Pyridoxal Phosphate; C005156:3-phosphoglycerate