title: A simple method for the determination of plasma extrinsic coagulant activity.
abstract: A spectrophotometric study of plasma coagulation is presented with a method for the determination of plasma coagulant activity, via the extrinsic pathway. Dilute citrated plasma was coagulated by recalcification after the addition of a very small amount of thromboplastin. The maximum increase in absorbance per min during coagulation was used as a measure of coagulant activity. The method was applied to normal platelet-rich plasma samples, and the effect of changing the concentration of plasma, thromboplastin, fibrinogen, and heparin was studied.
mesh_terms: D001780:Blood Coagulation Tests / Q000379:methods*; D005340:Fibrinogen; D006493:Heparin; D006801:Humans; D013053:Spectrophotometry; D013925:Thromboplastin; D013997:Time Factors
chemical_list: D005340:Fibrinogen; D006493:Heparin; D013925:Thromboplastin