title: The delineation of nuclear boundaries in microscopic images.
abstract: A semiautomatic procedure for tracking the boundaries of liver nuclei in digitized microscopic images of normally prepared biopsy material is described. The operator selects the nucleus, and a guided sequential search procedure tracks edge segments, which may subsequently be linked to produce a closed or partially open boundary, which is then accepted, completed or rejected by the user.
mesh_terms: D000465:Algorithms; D002467:Cell Nucleus / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000648:ultrastructure; D009685:Nuclear Envelope / Q000648:ultrastructure