Red cell Th activation: biochemical studies.
The peanut agglutinin from Arachis hypogea is a lectin that reacts with red blood cells expressing the Th antigen. The Th antigen has been said to be qualitatively similar to the T antigen, a well-defined antigen due to desialylation of glycophorin A and B that also reacts with the peanut agglutinin. We examined Th activated red blood cells from two patients with Fanconi's anaemia using 125I radiolabelled peanut agglutinin as a probe in Western blotting of red blood cell membrane proteins. We also probed the surface of intact Th activated red blood cells for structures related to the T antigen using [3H]sialic acid and a purified sialyltransferase. Neither of these techniques found antigens on the Th activated red blood cells that were similar to the antigen found on T activated red blood cells. These results show that the Th antigen in Fanconi's anaemia is qualitatively different to the antigen found in T activation.
D000741:Anemia, Aplastic / Q000097:blood*; D015295:Antigens, Tumor-Associated, Carbohydrate; D001789:Blood Group Antigens; D004187:Disaccharides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis / Q000276:immunology*; D005199:Fanconi Anemia / Q000097:blood*; D005260:Female; D006384:Hemagglutination; D006801:Humans; D007519:Isoantigens / Q000032:analysis; D037102:Lectins / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen / Q000032:analysis
D015295:Antigens, Tumor-Associated, Carbohydrate; D001789:Blood Group Antigens; D004187:Disaccharides; D007519:Isoantigens; D037102:Lectins; D008565:Membrane Proteins; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen; C038223:peanut agglutinin receptor; C016467:Thomsen-Friedenreich antigen