Metallic copper-containing post-column reactor for the detection of thiram and disulfiram in liquid chromatography.
A reaction detector has been developed for the selective detection of thiram and disulfiram. The detection is based on the post-column complexation of these analytes on a solid-state reactor packed with finely divided metallic copper to form a coloured copper complex, copper(II) N,N-dimethyldithiocarbamate, with an absorption maximum at 435 nm. The method is combined with a pre-concentration and clean-up step on a pre-column to permit the sub-ppb determination of, e.g., thiram in surface water samples or disulfiram in urine. Separation is achieved by reversed-phase liquid chromatography.
D002853:Chromatography, Liquid; D003300:Copper; D004221:Disulfiram / Q000032:analysis* / Q000652:urine; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet; D013859:Thiocarbamates / Q000032:analysis*; D013893:Thiram / Q000032:analysis*
D007202:Indicators and Reagents; D013859:Thiocarbamates; D013893:Thiram; D003300:Copper; D004221:Disulfiram