Glucagon-degrading activity in acid-ethanol extract of rat submandibular gland.
To investigate whether immunoreactive glucagon really exists in salivary gland, the integrity of glucagon radioimmunoassay was tested in the acid-ethanol extract of rat submandibular gland. Though immunoreactive glucagon was apparently measured in acid-ethanol extract of rat submandibular gland, the extract contained a significant amount of intact glucagon-degrading activity. The apparent % bound in radioimmunoassay highly correlated with the degradation of [125I] glucagon during incubation. Gel filtration profiles of [125I] glucagon incubated with acid-ethanol extract were the same as those of [125I] glucagon damaged by submandibular acid-saline extract. These data suggest that the immunoreactive glucagon in acid-ethanol extract is, as in the case of acid-saline extract, an artifact due to degradation of [125I] glucagon during radioimmunoassay.
D000818:Animals; D002850:Chromatography, Gel; D000431:Ethanol; D005934:Glucagon / Q000032:analysis*; D006851:Hydrochloric Acid; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013048:Specimen Handling; D013363:Submandibular Gland / Q000032:analysis*
D000431:Ethanol; D005934:Glucagon; D006851:Hydrochloric Acid