Post-meiotic transcription of phosphoglycerate-kinase 2 in mouse testes.
We have used a human phosphoglycerate kinase-1 (PGK-1) cDNA clone to study expression of PGK-2 during mouse spermatogenesis. Hybrid selection, in vitro translation with product identification by 2-D gel electrophoresis demonstrated that the PGK-1 cDNA clone hybridized to PGK-2 mRNA in mouse testes. Northern analyses of RNA purified from separated spermatogenic cells demonstrated a large increase in abundance of PGK-2 mRNA in post-meiotic cells. Thus, post-meiotic transcription of PGK-2 mRNA is demonstrable with cloned DNA probes.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008540:Meiosis; D051379:Mice; D009693:Nucleic Acid Hybridization; D010735:Phosphoglycerate Kinase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013045:Species Specificity; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D010735:Phosphoglycerate Kinase