Determination of serotonin in whole blood, platelet-rich plasma, platelet-poor plasma and plasma ultrafiltrate.
The important biogenic amine, serotonin (5HT), was determined in whole blood, platelet-rich plasma (PRP), platelet-poor plasma (PPP), and plasma ultrafiltrate after simple deproteinization. Following ion-pair chromatography on standard or narrow-bore reverse-phase columns, 5HT and the internal standard (N-methylserotonin-NMS) were detected by fluorometry with absolute detection limits of 2-4 pg. Levels obtained for whole blood and PRP were in agreement with previous methods. However, mean (+/- SD) values obtained for platelet-poor plasma (PPP) of 578 +/- 277 pg/ml (N = 7) were approximately 3-fold lower than the lowest previous reports. We also report the first determination of 5HT in plasma ultrafiltrate, having observed mean levels of 387 +/- 222 pg/ml (N = 7).
D000328:Adult; D000925:Anticoagulants / Q000097:blood; D001792:Blood Platelets / Q000032:analysis*; D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010949:Plasma / Q000032:analysis*; D012701:Serotonin / Q000097:blood*; D014462:Ultrafiltration
D000925:Anticoagulants; D012701:Serotonin