Effects of fetal calf serum and bovine serum albumin on in vitro maturation and fertilization of bovine and hamster cumulus-oocyte complexes.
Bovine serum albumin (BSA) and fetal calf serum (FCS) were evaluated as protein supplements for in vitro maturation and fertilization of oocytes from cows and hamsters. BSA and low doses of FCS (0.1 or 1.0%) did not support viability or maturation of the cumulus-oocyte complex as well as higher doses of FCS (5, 10, or 20%) for either species. BSA failed to support cumulus expansion for bovine or hamster cumulus-oocyte complexes. All doses of FCS examined supported cumulus expansion in bovine cumulus-oocyte complexes, whereas the hamster complexes required at least 1.0% FCS to induce cumulus expansion. The addition of a serum filtrate, Solcoseryl, with BSA improved viability of the cumulus in the bovine but did not support cumulus expansion or completion of Meiosis I in bovine complexes. In vitro fertilization could be accomplished in media containing FCS by increasing the heparin concentration in the bovine system or reducing FCS for the hamster system. Polyspermy was increased when FCS was the protein supplement. It is not known whether this is an interaction of FCS with the sperm or oocyte. In conclusion, FCS was found necessary for follicle-stimulating-hormone (FSH)-induced cumulus expansion. It also improved cumulus cell viability and completion of the first meiotic division in complexes of both species compared with BSA.
D000818:Animals; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D005260:Female; D005306:Fertilization / Q000187:drug effects; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000502:physiology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology
D003470:Culture Media; D012710:Serum Albumin, Bovine