[Study of ATP-independent stages of reaction catalyzed by phage T4 RNA-ligase].
The isotope exchange between [5'-32P]pAP and A(5')ppAp catalyzed by enzyme was shown not to take place in the absence of the acceptor; i. e. the necessity of the acceptor presence during the second step of the process was demonstrated. The isotope exchange reaction between [5'32P]pAp and (pA)5p was studied. It was demonstrated that acceptor (pA)4, slightly whereas the acceptor (pU)4 completely inhibits the isotope reaction. The isotope reaction exchange between [5'-32P]pAp and (pU)4pAp does not take place. The question of existence of adenylated donor elimination mechanism in the presence of "poor" acceptors is considered on the basis of the data obtained.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D011061:Poly A / Q000378:metabolism; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D013604:T-Phages / Q000201:enzymology*
D011061:Poly A; D000255:Adenosine Triphosphate; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)
Izuchenie ATP-nezavisimykh stadiĭ reaktsii, kataliziruemoĭ RNK-ligazoĭ bakteriofaga T4.