[Effect of neuraminidase and x-rays (2 Gy and 8 Gy) on microvilli and membrane invaginations of Ehrlich ascites tumor cells in monolayer culture].
A monolayer culture (Eagle basal medium plus 10% of fetal calf serum) of Ehrlich ascites tumor cells was exposed to X-radiation with 2 Gy and 8 Gy and treated with Vibrio cholerae neuraminidase alone or combined with sublethal X-ray irradiation (2 Gy). Pictures of the Ehrlich ascites tumor cells taken with the electron microscope were investigated in order to find out any cell surface modifications due to membrane invaginations and microvilli. The results showed that the rate of microvilli as well as that of membrane invaginations became higher with the increasing X-ray dose (2 Gy; 8 Gy). Following to neuraminidase treatment there was a considerable augmentation of membrane invaginations as compared to control cells, whereas the number of microvilli was slightly reduced. As it has been already described before, the influence of neuraminidase produced an increased endocytosis activity and a strengthening of the cytoskeleton. Combined treatment with neuraminidase and sublethal X-radiation (2 Gy) caused a higher rate of membrane invaginations than each method alone; the number of microvilli was slightly increased by combined treatment. The conclusion is drawn that these structure modifications are due to reparation processes induced by radiation on the one hand and to an enzymic action of neuraminidase on the cell surface on the other hand.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000187:drug effects / Q000528:radiation effects*; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008871:Microvilli / Q000187:drug effects / Q000528:radiation effects; D009439:Neuraminidase / Q000494:pharmacology*
D009439:Neuraminidase
Einfluss von Neuraminidase und Röntgenbestrahlung (2 Gy und 8 Gy) auf Mikrovilli und Membraninvaginationen von Ehrlich-Aszites-Tumorzellen in Monolayerkultur.