title: Isolation and partial characterization of early pregnancy factor (EPF) from human pregnancy serum.
abstract: The isolation and partial characterization of an immunosuppressive early pregnancy factor (EPF) present in the serum of pregnant women, between 3 and 8 weeks of gestation, is described. EPF was purified using ion-exchange chromatography, affinity chromatography and HPLC gel permeation techniques. A homogeneous, active fraction containing a single polypeptide of Mr 21,000 was obtained. This 21 kDa polypeptide appears to represent the major form of rosette-inhibiting active material present in maternal serum during early human gestation.
mesh_terms: D018835:Chaperonin 10; D006063:Chorionic Gonadotropin / Q000097:blood; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000302:isolation & purification*; D010455:Peptides; D011247:Pregnancy / Q000276:immunology*; D011257:Pregnancy Proteins; D011261:Pregnancy Trimester, First; D013491:Suppressor Factors, Immunologic
chemical_list: D018835:Chaperonin 10; D006063:Chorionic Gonadotropin; D007166:Immunosuppressive Agents; D010455:Peptides; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; C025124:early pregnancy factor