Characterization of the binding of the anti-sickling compound, BW12C, to haemoglobin.
The anti-sickling agent BW12C [Beddell, Goodford, Kneen, White, Wilkinson & Wootton (1984) Br. J. Pharmacol. 82, 397-407] was designed to left-shift the oxygen saturation curve of haemoglobin (HbA) by preferential binding to the oxy conformation at a single site between the terminal amino groups of the alpha-chains through Schiff's base formation, ionic and hydrophobic interactions. In the present work, Schiff's base linkages formed with [14C]BW12C were reduced with NaBH4 and the alpha- and beta-globin chains separated. Under oxy conditions at a molar ratio of 2:1, the covalently bound BW12C is localized almost exclusively on a single alpha-chain; tryptic digestion confirms the terminal amino group (alpha 1-valine) as the reaction site, in accord with the design hypothesis. However, about half the labelled BW12C is released on tetramer disruption, suggesting the presence of additional non-covalent binding. Under deoxy conditions, alpha- and beta-chains are labelled approximately equally, and at higher molar ratios additional binding in both oxy and deoxy conditions is seen. Isoelectric-focusing studies under oxy conditions show a complex pattern of modified bands for both HbA and HbA1c (blocked beta-terminal amino groups) but no modification for HbA carbamylated at both alpha- and beta-terminal amino groups or at the alpha-chains only, again confirming the alpha-terminal amino region as the main interaction site. Equilibrium dialysis measurements under oxy conditions indicate two strong binding sites with a binding constant of less than 10(-6) M and a number of weaker binding sites. The present data thus confirm that BW12C binds at the intended locus but reveal additional non-covalent binding at an undefined site, and weaker binding through Schiff's base formation with other amino groups.
D000447:Aldehydes / Q000378:metabolism*; D001547:Benzaldehydes; D001665:Binding Sites; D001894:Borohydrides; D003956:Dialysis; D006441:Hemoglobin A / Q000378:metabolism*; D006801:Humans; D007525:Isoelectric Focusing; D010084:Oxidation-Reduction; D012545:Schiff Bases
D000447:Aldehydes; D001547:Benzaldehydes; D001894:Borohydrides; D012545:Schiff Bases; C039599:5-(2-formyl-3-hydroxyphenoxy)pentanoic acid; C025364:sodium borohydride; D006441:Hemoglobin A