Multiple form of L-phenylalanine sensitive alkaline phosphatase in rat fecal extracts.
Alkaline phosphatase activity was detected in fecal extracts of male rats. 58% of the total enzyme activity was inhibited by 30 mM L-phenylalanine, which is an inhibitor specific for intestinal alkaline phosphatase. L-Phenylalanine sensitive alkaline phosphatase in the fecal extracts revealed multiple peaks on DEAE-Sephadex A-25 ion exchange column with a linear gradient of NaCl. It is discussed that a part of the fecal alkaline phosphatase might be a set of catabolites of the intestinal enzyme.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000818:Animals; D001419:Bacteria / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D005243:Feces / Q000201:enzymology*; D007422:Intestines / Q000201:enzymology / Q000382:microbiology; D008297:Male; D010649:Phenylalanine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D010649:Phenylalanine; D000469:Alkaline Phosphatase