Antiplatelet antibody determination using platelet factor 3 assay in thrombocytopenic patients.
Platelet factor 3 assay was used to detect antiplatelet antibodies in thrombocytopenic patients with platelet count less than 100,000/cu.mm. A total of 121 specimens was tested. The results gave a positive rate of 83% (61/74) for idiopathic (immune) thrombocytopenic purpura (ITP), 67% for systemic lupus erythematosus (SLE) and 54% (15/28) for others with & 13% (2/16) without history of blood components therapy. The sera from SLE without thrombocytopenic were also checked for the antibody, and a 14% positive rate was obtained. Patients with ITP with complete remission and normal platelet count were 40% positive. Antiplatelet antibody determination by platelet factor 3 assay is a sensitive and reproducible method. It can be performed in most laboratories and requires no specific equipment.
D001323:Autoantibodies / Q000032:analysis*; D001779:Blood Coagulation Factors / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D010977:Platelet Factor 3 / Q000032:analysis*; D011696:Purpura, Thrombocytopenic / Q000276:immunology; D013921:Thrombocytopenia / Q000276:immunology*
D001323:Autoantibodies; D001779:Blood Coagulation Factors; D010977:Platelet Factor 3