A simple and sensitive solid-phase radioimmunoassay for measuring the transferrin content of human biological fluids: its application to seminal plasma.
A highly sensitive and reproducible radioimmunoassay was established to detect transferrin in human fluids. By this technique, applied to seminal fluid, transferrin levels (micrograms/ml) were found in normozoospermic individuals (64.49 +/- 25.41) at level higher than in oligozoospermic (38.93 +/- 21.35), azoospermic (19.49 +/- 10.23), or vasectomized (19.61 +/- 8.95) subjects. A relationship between transferrin and spermatozoid concentration in sperm was shown. These results reinforce previous findings that seminal transferrin can be used as a reliable clinical marker of Sertoli Cell function.
D001826:Body Fluids / Q000032:analysis*; D019411:Clinical Laboratory Techniques; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012661:Semen / Q000032:analysis*; D014168:Transferrin / Q000032:analysis*; D014659:Vasectomy
D014168:Transferrin