Effect of opsonization on oxidative metabolism of plaice (Pleuronectes platessa L.) neutrophils.
The effect of serum opsonization on Vibrio alginolyticus (heat-killed)-stimulated chemiluminescence (CL) by plaice kidney- and peritoneal exudate-derived neutrophils was investigated. Peritoneal neutrophils only recognized heat-labile and kidney neutrophils only heat-stable opsonic activity in normal serum. Specific antibody did not show opsonic activity nor any synergism with the normal serum opsonins for either neutrophil population. Evidence was found for the production, by plaice neutrophils, of H2O2, O2-, OH. and two or more, as yet unidentified, reactive oxygen species (ROS).
D000818:Animals; D005412:Flatfishes / Q000378:metabolism*; D005432:Flounder / Q000276:immunology / Q000378:metabolism*; D005609:Free Radicals; D007668:Kidney / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D009895:Opsonin Proteins / Q000276:immunology / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis; D014733:Vibrio / Q000276:immunology
D005609:Free Radicals; D009895:Opsonin Proteins; D010100:Oxygen