Transferrin variants in sheep: separation and characterization by polyacrylamide gel electrophoresis and isoelectric focusing.
Isoelectric focusing with carrier ampholytes in ultrathin polyacrylamide gels and polyacrylamide gel electrophoresis in a discontinuous buffer system were used for the separation of sheep transferrin variants. For identification of the different iron-binding sites of transferrin a stepwise urea gradient, different degrees of iron saturation and double one-dimensional electrophoresis were used. Isoelectric focusing results in an increased resolution of the Fe0-transferrin, Fe1-transferrin and Fe2-transferrin region. At the level of Fe0-transferrin and Fe1-transferrin the variants I, A, G, B, C, D, M, E, Q, P can be identified. The method is especially suitable for genetic studies. For screening purposes up to 108 samples can be separated within one run in an ultrathin gel.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D007525:Isoelectric Focusing; D011110:Polymorphism, Genetic; D012756:Sheep / Q000097:blood / Q000235:genetics*; D014168:Transferrin / Q000235:genetics* / Q000302:isolation & purification
D014168:Transferrin