The amino-terminal sequence of the 85-90K nonhormone binding component of the molybdate-stabilized estradiol receptor from calf uterus.
The first six N-terminal amino acid residues of the 85-90K non-estrogen binding component of the calf uterine, molybdate-stabilized estradiol receptor have been determined by Edman degradation. After affinity chromatography of the stabilized receptor oligomer, the 85-90K unit was purified to homogeneity by preparative gel electrophoresis using electroelution for protein recovery. Inverse-gradient high performance liquid chromatography provided the 85-90K protein suitable for amino-terminal sequence analysis.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D005260:Female; D008970:Molecular Weight; D008982:Molybdenum / Q000494:pharmacology*; D011485:Protein Binding; D011959:Receptors, Estradiol / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; C044659:molybdate; D008982:Molybdenum