Hepatic estrogen receptor in the turtle, Chrysemys picta: partial characterization, seasonal changes and pituitary dependence.
A hepatic estrogen receptor is described from female turtles, Chrysemys picta. The receptor adheres to DNA after incubation with [3H]estradiol and can be eluted with a linear salt gradient as a single component with an elution maximum of 0.21 M. It is steroid-specific, binding estrogens, but not androgens or progestins. Specific binding saturates between 3 and 7 nM [3H]estradiol-17 beta and Scatchard analysis gave a Kd of 2 X 10(-9) M and a maximal binding capacity of 3.02 fmol/mg protein. Hypophysectomy reduces hepatic estradiol receptor from 70 fmol/g tissue in control animals to non-detectable levels. Growth hormone replacement partially restored the receptor to 36% of control. Significant changes in receptor occur during the ovarian cycle.
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology; D008099:Liver / Q000502:physiology*; D008297:Male; D008970:Molecular Weight; D010902:Pituitary Gland / Q000502:physiology; D011960:Receptors, Estrogen / Q000502:physiology*; D012621:Seasons; D014018:Tissue Distribution; D014426:Turtles / Q000502:physiology*
D004268:DNA-Binding Proteins; D011960:Receptors, Estrogen; D013006:Growth Hormone