Uptake of cadmium in isolated kidney cells--influence of binding form and in vivo pretreatment.
Uptake of cadmium as 109CdCl2, 109Cd-cysteine, 109Cd-albumin and 109Cd-metallothionein was studied in isolated kidney cells from rat. Cd as 109CdCl2 and 109Cd-albumin was taken up at similar rates. The uptake of cadmium as 109Cd-cysteine was greater and that of 109Cd-metallothionein lower compared with that of the other substances. These observations were made on non-pretreated cells. In cells taken from rats pretreated with CdCl2 in vivo, the uptake of cadmium as 109CdCl2, 109Cd-cysteine and 109Cd-albumin was lower than in cells from non-pretreated rats. However, the uptake of 109Cd-metallothionein was considerably enhanced in pretreated cells. In pretreated kidney cells the decreased uptake of Cd (as Cd-albumin) might be related to protection of the kidney against acute Cd toxicity and increased uptake of metallothionein-Cd might contribute to the explanation of renal damage in long-term Cd exposure.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000378:metabolism* / Q000633:toxicity; D003545:Cysteine / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000418:Albumins; D002104:Cadmium; D008668:Metallothionein; D003545:Cysteine