Effect of hyperthermia on the repair of sublethal radiation damage in normal and membrane fatty acid substituted fibroblasts.
The interaction of heat and X irradiation was studied in normal and polyunsaturated fatty acid (PUFA) substituted mouse fibroblast LM cells. As a result of the substitution the membranes of the PUFA cells were more fluid than the membranes of the normal cells. Three different heat doses were applied (60 min 42 degrees C, 20 min 43 degrees C, and 10 min 44 degrees C) in combination with single or split doses of X rays. Heat radiosensitization was the largest for the 60 min 42 degrees C treatment. Heat radiosensitization and the heat-induced inhibition of the rate of sublethal damage repair were the same for the normal and the PUFA cells. It is concluded from the experiments reported that the processes of hyperthermic inhibition of SLD repair and hyperthermic radiosensitization are independent of membrane fluidity and membrane fatty acid composition.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D004260:DNA Repair; D005227:Fatty Acids / Q000378:metabolism*; D005347:Fibroblasts / Q000528:radiation effects*; D006358:Hot Temperature; D008560:Membrane Fluidity / Q000528:radiation effects; D051379:Mice; D008809:Mice, Inbred C3H; D011835:Radiation Protection
D005227:Fatty Acids