3H-adrenaline release from rabbit isolated aorta by electrical-field stimulation.
The aim of the present work was to study the release of 3H-adrenaline (3H-A) from rabbit isolated aorta. This release was compared with that of 3H-noradrenaline (3H-NA). The stimulation-evoked 3H-overflow from aorta preloaded with 3H-A decreased with repeated stimulation (S1-S3). In contrast, prestimulation enhanced subsequent stimulation-evoked 3H-overflows. For both 3H-amines, the 3H-overflow increased concomitantly to the same degree with the number of pulses (30-1000) at 3 Hz. The time course of 3H-overflows with either 3H-A or 3H-NA was compared. In the case of 3H-A, the 3H-overflow increased with time, while for 3H-NA the 3H-overflow remained unchanged both at 3 and 10 Hz. The stimulation-evoked 3H-overflow from rings preloaded with either 3H-amine was the same at frequencies from 1 to 10 Hz, whereas at 30 Hz the 3H-overflow derived from 3H-A was higher than that using 3H-NA. An analysis of the quantitative aspects of individual stimulation-evoked 3H-overflows showed that the fractional profile did not differ using either amine. In both cases, at least 96% of the 3H-overflow evoked by stimulation at 3 or 10 Hz was contained in the initial four 2-min. fractions. The ratio between stimulation-evoked 3H-overflow and spontaneous 3H-outflow was the same for both amines at 3 Hz, while the ratio was higher for 3H-NA at 10 Hz. Rauwolscine (10(-6) M) enhanced the 3H-overflow 6-fold, while (-)-propranolol (10(-7) M) had no effect at 3 Hz.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001808:Blood Vessels / Q000294:innervation / Q000378:metabolism*; D003042:Cocaine / Q000494:pharmacology; D003345:Corticosterone / Q000494:pharmacology; D004558:Electric Stimulation; D004837:Epinephrine / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D011817:Rabbits; D015016:Yohimbine / Q000494:pharmacology
D015016:Yohimbine; D011433:Propranolol; D003042:Cocaine; D003345:Corticosterone; D009638:Norepinephrine; D004837:Epinephrine