Glucocorticoids change the nucleotide and sugar nucleotide pool sizes in cultured human skin fibroblasts.
Fibroblasts in culture were treated with the glucocorticoid budesonide. The nucleotide and sugar nucleotide pools were quantitated after separation by isotachophoresis. Steroid treatment induced a 40% increase of the UDP-N-acetylhexosamine pool and a 30% increase of the UDP-glucuronic acid pool whereas the UTP pool was diminished. These effects became apparent after 24 h of incubation and a new steady state was attained after 48 h of incubation. The 3'-phosphoadenosine-5'-phosphosulfate pool was probably not influenced by the glucocorticoid treatment. The half-life of the UDP-N-acetylhexosamine pool was considerably longer in treated than in control cells. The efflux from the UDP-N-acetylhexosamine pool was also lowered in the treated cells. The changed efflux may be due to a decreased glycoconjugate synthesis induced by glucocorticoid treatment. The rate of equilibration of [14C]glucose and [3H]glucosamine with the UDP-N-acetylhexosamine pool was changed by glucocorticoid treatment; especially that of [3H]glucosamine was decreased.
D019819:Budesonide; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005938:Glucocorticoids / Q000494:pharmacology*; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D009711:Nucleotides / Q000378:metabolism*; D011282:Pregnenediones / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D014544:Uridine Triphosphate / Q000378:metabolism
D005938:Glucocorticoids; D009711:Nucleotides; D011282:Pregnenediones; D013431:Sulfates; D014535:Uridine Diphosphate Glucuronic Acid; D019819:Budesonide; D014537:Uridine Diphosphate N-Acetylglucosamine; D014536:Uridine Diphosphate N-Acetylgalactosamine; D005947:Glucose; D005944:Glucosamine; D014544:Uridine Triphosphate