A versatile perfusion technique for metabolic studies by NMR.
A perfusion technique applicable to metabolic studies by NMR is described in detail. The utility and versatility of the approach are demonstrated by following gluconeogenesis from [2-13C]pyruvate in the perfused, isolated mouse liver, and lipogenesis from [2-13C]acetate in perfused Acanthamoeba castellanii cells embedded in agarose filaments.
D000085:Acetates / Q000378:metabolism; D000656:Amoeba; D000818:Animals; D005943:Gluconeogenesis; D066298:In Vitro Techniques; D008055:Lipids / Q000096:biosynthesis; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008660:Metabolism; D051379:Mice; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid
D000085:Acetates; D008055:Lipids; D011773:Pyruvates; D019289:Pyruvic Acid