Distinct patterns of expression of different protein kinase C mRNAs in rat tissues.
The identification of multiple protein kinase C (PKC) cDNA sequences from rat, bovine, and human tissues has led to the discovery of a family of PKC genes. Using probes complementary to three cDNA sequences from rat brain, designated PKC-I, -II, and -III, the distribution of PKC transcripts in rat tissues was studied by in situ hybridization histochemistry. In brain, PKC-II and PKC-III transcripts colocalized except in the brain stem and spinal cord, where some cells appeared to contain only PKC-III message. PKC-I mRNA had a different, but partially overlapping, pattern of expression. In spleen, PKC-II and PKC-III, but not PKC-I, transcripts were concentrated in the marginal zone of white pulp, the major B-lymphocyte compartment of rat spleen. The distinct distributions of rat PKC mRNAs suggest that different members of the PKC family have specialized functions.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D006801:Humans; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D011493:Protein Kinase C / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D013045:Species Specificity; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004247:DNA; D011493:Protein Kinase C