Active H-ras and N-ras in rat fibrosarcomas induced by 1,6-dinitropyrene.
The activated oncogenes in rat fibrosarcomas induced by subcutaneous injection of 1,6-dinitropyrene (1,6-DNP), an environmental mutagen-carcinogen, were examined by NIH3T3 cell transfection assay and Southern blot analysis. DNAs from two of eleven fibrosarcomas, 1,6-DNP6 and 1,6-DNP9T, induced transformants which contained rat specific repetitive sequences. Three primary transformants, obtained with DNA from 1,6-DNP6, and two secondary transformants of one of these primary transformants tested, contained rat H-ras. DNA from 1,6-DNP9T; a fourth transplant of 1,6-DNP9, also induced transformants. All the primary transformants with DNA from 1,6-DNP9T, and three secondary transformants of one of them, contained rat N-ras. The DNA from the original tumor, 1,6-DNP9, however, did not give any transformants by several transfection assays. Activation of N-ras oncogene during serial transplantations is discussed.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D005354:Fibrosarcoma / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D009153:Mutagens; D009857:Oncogenes; D011721:Pyrenes / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection
D009153:Mutagens; D011721:Pyrenes; C033309:1,6-dinitropyrene; D004247:DNA