[A modified highly sensitive spectrophotometric method for determining acid phosphatase activity in the animal liver].
A modified spectrophotometric procedure is described for estimation of acid phosphatase activity in rabbit liver tissue. Coefficient of instrumental sensitivity (SA/C = 0.029), apparent molar absorption (epsilon 820 = 30,500), relative sensitivity of measurement (Cmin = 0.18 mM) were calculated as well as main metrological patterns were evaluated, which showed that the procedure was highly sensitive, accurate and reproducible. Standard error did not exceed 1% and standard deviation was not above 5%.
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D008099:Liver / Q000201:enzymology*; D008297:Male; D011817:Rabbits; D013053:Spectrophotometry
D000135:Acid Phosphatase
Modifitsirovannyĭ vysokochuvstvitel'nyĭ spektrofotometricheskiĭ metod opredeleniia aktivnosti kisloĭ fosfatazy iz pecheni zhivotnykh.