[Determination of indole and skatole in seafood using high performance liquid chromatography (HPLC)].
A rapid and specific high-performance liquid chromatographic (HPLC) method for the determination of indole and 3-methylindole (skatole) is described. The detection limit of the two compounds is approx. 0.02 mg/kg. Gas chromatographic-mass spectrometric (GC-MS) confirmation of the identity has been carried out in shrimps. About 95% of added indole was recovered. The indole formation in shrimps, herring, and turbot, held under different storage conditions, is compared with other common quality indices such as TBA-value and alpha-tocopherol content.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D033364:Decapoda / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007211:Indoles / Q000032:analysis*; D012758:Shellfish / Q000032:analysis*; D012862:Skatole / Q000032:analysis*
D007211:Indoles; D012862:Skatole
Bestimmung von Indol und Skatol in Meerestieren durch Hochleistungs-Flüssigchromatographie (HPLC).