Blood antioxidant status and plasma pyruvate kinase activity of halothane-reacting pigs.
Samples of blood were collected (by jugular venipuncture) from 10 homozygote halothane-reacting pigs and 7 homozygote nonreactors. Antioxidant status was assessed by measurement of blood glutathione peroxidase, glucose-6-phosphate dehydrogenase, and superoxide dismutase activities and plasma concentrations of vitamin E. Significant differences in these values were not apparent between halothane-reacting pigs and nonreactors. Halothane-reacting pigs had tissue damage as indicated by significantly increased plasma activities of the enzymes creatine kinase and pyruvate kinase. The latter showed better discrimination between the 2 populations than did creatine kinase and could prove to be a more effective marker for identification of stress-susceptible pigs.
D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006221:Halothane / Q000494:pharmacology*; D011770:Pyruvate Kinase / Q000097:blood*; D013312:Stress, Physiological / Q000097:blood / Q000201:enzymology / Q000662:veterinary*; D013482:Superoxide Dismutase / Q000097:blood; D013552:Swine / Q000097:blood*; D013553:Swine Diseases / Q000097:blood* / Q000201:enzymology; D014810:Vitamin E / Q000097:blood
D014810:Vitamin E; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D011770:Pyruvate Kinase; D003402:Creatine Kinase; D006221:Halothane