Peroxisomes in lymphatic leukemia P 388 in mice--cytoenzymatic and ultrastructural investigations.
The study comprised mice of BDF1 hybrides, i.e. cross-breds F1/C57BL/6 X DBA/2, sires, aged 3 months, injected peritoneally with lymphatic leukemia P 388 in the amount of 10(4) cells. The mice were killed 5 or 11 days after injection. Peroxisomes were examined in the liver. Therefore, cytoenzymatic studies were conducted to determine the catalase activity. It was found that, after initial increase of activity (5th day of the experiment) a total or nearly total lack of catalase occurred in leukemic lymphocytes after 11 days of the disease. This was accompanied by gradual vanishing of peroxisomes-catalase transfer. It was also found that free radicals participated actively in the destabilisation of biological membranes by damaging cellular peroxisomes.
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D005609:Free Radicals; D007941:Leukemia P388 / Q000201:enzymology* / Q000473:pathology; D007942:Leukemia, Experimental / Q000201:enzymology*; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008830:Microbodies / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron
D005609:Free Radicals; D002374:Catalase