Lysostaphin lysis procedure for detection of Staphylococcus aureus by the firefly bioluminescent ATP method.
The objective of this study was to examine the use of lysostaphin as an ATP-extracting agent for the estimation of Staphylococcus aureus cell number by a rapid bioluminescent ATP method. The results of the study showed that lysostaphin (22 U/ml) was able to lyse most of the S. aureus cells (greater than 99.9%) at room temperature in 1 min; ATP of S. aureus cells extracted by the lysostaphin lysis procedure was stable for 24 h in the presence of EDTA; there was a linear relationship between the ATP content and the number of S. aureus cells (ranging from 10(4) to 10(6) CFU/ml); and the lysis of S. aureus cells by lysostaphin allowed estimation of the number of S. aureus cells in mixed cultures and in meat samples.
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001433:Bacteriolysis; D002417:Cattle; D005516:Food Microbiology; D008248:Lysostaphin / Q000378:metabolism*; D008460:Meat; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000378:metabolism
D000255:Adenosine Triphosphate; D008248:Lysostaphin