Simultaneous determination of biogenic amines and morphine in discrete rat brain regions by high-performance liquid chromatography with electrochemical detection.
A simple and sensitive method has been developed for the simultaneous determination of norepinephrine, epinephrine, dopamine, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, and morphine in discrete rat brain regions by reversed-phase high-performance liquid chromatography with electrochemical detection. Perchloric acid extracts of the tissue were directly injected into the chromatographic system. Each of these compounds gave a linear response over the range of 20-160 ng/ml cerebellar homogenate (0.4-3.2 ng on column). Recoveries of these compounds, added to the homogenates, were complete when compared with standards dissolved in perchloric acid. The average between-run coefficients of variation for all these compounds were lower than 7.4% over the range of 20-160 ng/ml, and the within-run coefficients of variation at 20 ng/ml were lower than 8.7%. The present method has been applied to a study of the effects of intraperitoneal administration of morphine on biogenic amines in several discrete rat brain regions.
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D008297:Male; D009020:Morphine / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D001679:Biogenic Amines; D009020:Morphine