Type-A retrovirus particles in heterohybridomas: precaution for human monoclonal antibody purification.
Fine structural features of the murine myeloma MHFP-1 and two heterohybridomas secreting human IgM monoclonal antibody were examined. Intracisternal type-A retrovirus particles were found in both MHFP-1 and the heterohydribomas constructed by fusing MHFP-1 and human peripheral blood lymphocytes. The implications of this finding for the purification of human monoclonal antibody for therapeutic applications is discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D010954:Plasmacytoma / Q000382:microbiology / Q000648:ultrastructure; D012190:Retroviridae / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal