Cytogenetic analysis of leukaemic cells in the dog. A report of 10 cases and a review of the literature.
Cytogenetic abnormalities were found in 9 of 10 leukaemic dogs. Important findings were aneuploidy, especially hyperdiploidy, extra-metacentric chromosomes, double minute chromosomes and tetraploidy. Six dogs had a normal chromosome modal number of 78, but 4 other dogs had modal numbers of 54, 81, 83 or 85 and 156. One to 16 extra-metacentric chromosomes were seen in cells from 5 dogs. Double minute chromosomes, which appear to be markers for neoplasia, were seen in one or more cells from 3 leukaemic dogs. Too few adequate, simultaneous studies were available to compare cytogenetic results from blood and bone marrow samples of leukaemic dogs. Extra-metacentric chromosomes and double minute chromosomes were not found in cells from the normal control dogs and cells with more than 78 chromosomes were rare. Specific chromosome changes were not associated with any of the various types of leukaemia and prognosis could not be predicted from the cytogenetic findings but, in general, the prognosis was poor. However, it does appear that many or most dogs with leukaemia have chromosomal abnormalities. With additional research and improvements in leukaemia therapy, cytogenetic studies of canine leukaemia may provide diagnostic and prognostic information.
D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics / Q000662:veterinary*; D007962:Leukocytes / Q000166:cytology; D008297:Male; D009919:Orchiectomy; D010052:Ovariectomy; D012016:Reference Values