Enhanced chemiluminescent reactions catalyzed by horseradish peroxidase.
Enhancement of light emission from the horseradish peroxidase-catalyzed oxidation of diacyl hydrazides on addition of 6-hydroxybenzothiazole or phenol derivatives forms the basis of rapid, specific, and sensitive chemiluminescent assays for peroxidase. The advantages and wide applicability of the technique have been demonstrated in a range of ligand-binding assays. Careful selection of chemiluminescent reagents, enhancer, their relative proportions and reaction conditions, and more detailed knowledge of the mechanism of enhancement should enable further improvements in sensitivity and the intensity or constancy of light emission.
D004077:Digoxin / Q000032:analysis; D006735:Horseradish Peroxidase / Q000032:analysis*; D006861:Hydrogen Peroxide; D007073:Immunoglobulin E / Q000032:analysis; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008722:Methods; D010544:Peroxidases / Q000032:analysis*; D011506:Proteins / Q000032:analysis
D007202:Indicators and Reagents; D011506:Proteins; D007073:Immunoglobulin E; D008165:Luminol; D004077:Digoxin; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases