[Molecular mechanisms of photoreception. V. Isolation of cytoplasmic membranes of rod outer segments from the frog retina].
Cytoplasmic membranes of rod outer segments from frog retina intact rods in retina were stained with fluorescent dye fluoresceinmonomercur acetate. The dye is covalently bound to proteins of cytoplasmic membrane and doesn't penetrate into the cells. Upon isolation of the purified outer segments with the labeled cytoplasmic membranes the cells were disrupted and fractionated in density sucrose gradient. Cytoplasmic membranes possess floating densities different from those of disk membranes and thus providing a mean for separating them from the latter. The main peptides of cytoplasmic membranes are 56, 53, 45, 30 and 28 kDa proteins.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis*; D005452:Fluoresceins; D008565:Membrane Proteins / Q000032:analysis; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D010786:Photoreceptor Cells / Q000032:analysis*; D011895:Rana ridibunda; D012374:Rod Cell Outer Segment / Q000032:analysis* / Q000648:ultrastructure
D005452:Fluoresceins; D008565:Membrane Proteins; D010455:Peptides
Molekuliarnye mekhanizmy fotoretseptsii. V. Vydelenie tsitoplazmaticheskikh membran naruzhnykh segmentov palochek iz setchatki liagushki.