[Metabolic transformation of labelled exogenous fatty acids by fungal cultures of the family Entomophthoraceae].
Entomophthora coronata 1932 and E. conica 1716 are quite different in their fatty acid composition and the unsaturation degree of synthesized lipids. The cultures were used as models to study metabolic transformations of exogenous 14C-labeled acetic, palmitic, stearic and oleic acids as well as to compare the activities of the synthetase and desaturase enzyme complexes. The cultures were capable of transforming exogenous acetic and fatty acids into polyunsaturated arachidonic acid. E. coronata 1932 whose lipids mainly contain fatty acids with a short chain could metabolize unsaturated oleic acid to yield polyene fatty acids. However, this culture metabolized exogenous acids at a far lower rate as compared with E. conica 1716. The high content of saturated fatty acids with a short chain in the lipids might be due to the specific action of the synthetase complex and to the low activity of the desaturation enzymes. It has been demonstrated for the first time that exogenous oleic acid is converted at a high rate by the cells into arachidonic acid, a precursor of prostaglandin compounds.
D001711:Biotransformation; D002250:Carbon Radioisotopes; D003470:Culture Media / Q000378:metabolism; D004773:Entomophthora / Q000201:enzymology*; D044943:Fatty Acid Desaturases / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology*
D002250:Carbon Radioisotopes; D003470:Culture Media; D005227:Fatty Acids; D044943:Fatty Acid Desaturases; D064429:Fatty Acid Synthases
Metabolicheskie prevrashcheniia ékzogennykh mechenykh zhirnykh kislot kul'turami gribov semeĭstva Entomophthoraceae.