Comparison of methods for assessing photoprotection against ultraviolet A in vivo.
Photoprotection against ultraviolet A (UVA) by three sunscreens was evaluated in humans, with erythema and pigmentation used as end points in normal skin and in skin sensitized with 8-methoxypsoralen and anthracene. The test sunscreens were Parsol 1789 (2%), Eusolex 8020 (2%), and oxybenzone (3%). UVA was obtained from two filtered xenon-arc sources. UVA protection factors were found to be significantly higher in sensitized skin compared with normal skin. Both Parsol and Eusolex provided better and comparable photoprotection (approximately 3.0) than oxybenzone (approximately 2.0) in sensitized skin, regardless of whether 8-methoxypsoralen or anthracene was used. In normal unsensitized skin, Parsol 1789 and Eusolex 8020 were also comparable and provided slightly better photoprotection (approximately 1.8) than oxybenzone (approximately 1.4) when pigmentation was used as an end point. The three sunscreens, however, were similar in providing photoprotection against UVA-induced erythema. Protection factors obtained in artificially sensitized skin are probably not relevant to normal skin. It is concluded that pigmentation, either immediate or delayed, is a reproducible and useful end point for the routine assessment of photoprotection of normal skin against UVA.
D000293:Adolescent; D000328:Adult; D000873:Anthracenes / Q000494:pharmacology; D001565:Benzoates / Q000627:therapeutic use; D001577:Benzophenones / Q000627:therapeutic use; D047188:Chalcones; D004890:Erythema / Q000209:etiology; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D008730:Methoxsalen / Q000494:pharmacology; D008875:Middle Aged; D011427:Propiophenones; D012880:Skin Pigmentation / Q000187:drug effects / Q000528:radiation effects; D013471:Sunburn / Q000517:prevention & control*; D013473:Sunscreening Agents / Q000627:therapeutic use*; D014466:Ultraviolet Rays / Q000009:adverse effects
D000873:Anthracenes; D001565:Benzoates; D001577:Benzophenones; D047188:Chalcones; D011427:Propiophenones; D013473:Sunscreening Agents; C049936:4-isopropyldibenzoylmethane; C005290:oxybenzone; C049935:avobenzone; D008730:Methoxsalen