Polarimetric assay for the determination of the native collagen content of soluble collagen.
Two standardized assays for determining the amount of native collagen in a collagen solution are described which are based on polarimetric measurements. Native collagen content is calculated from the loss of optical rotation during transition from the original to the denatured conformation using constants which were obtained from standard collagen of highest available native collagen content. While Method I gives information on the content of helical collagen, Method II estimates collagen which is resistant to peptic degradation. Both methods are simple to perform and suitable for routine quality control.
D000818:Animals; D003094:Collagen / Q000032:analysis; D009904:Optical Rotation; D011487:Protein Conformation; D011489:Protein Denaturation; D012995:Solubility
D003094:Collagen