[Effect of various thiamine supplies of the body on the enzyme activity in the metabolism of xenobiotics and lipid peroxidation in rat liver microsomes].
The effect of different thiamine supply of the organism on the enzymic activity in metabolism of xenobiotics and the processes of the lipid peroxidation in the liver microsomes with the application of phenobarbital, an inductor of microsomes' enzymes are studied in experiments on Wistar albino male rats. It is established that deficit of vitamin B1 increases the activity in most of processes studied in microsomes and also the intensity of lipids' peroxidation. Phenobarbital enhances the activity of microsomal oxidation irrespectively of vitamin B1 supply, whereas peroxidation of lipids is activated by phenobarbital only in animals fed on physiological doses of vitamin B1. The N-demethylation rate of dimethylaniline in experiments in vitro is inhibited by high doses of thiamine (150 microM), its derivatives inhibited this process in low concentrations (15 microM) as well.
D000818:Animals; D001711:Biotransformation; D004790:Enzyme Induction; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013832:Thiamine Deficiency / Q000201:enzymology / Q000378:metabolism*; D014174:Transketolase / Q000378:metabolism
D008054:Lipid Peroxides; D010088:Oxidoreductases; D014174:Transketolase; D010634:Phenobarbital
Vliianie razlichnoĭ obespechennosti organizma tiaminom na aktivnost' fermentov metabolizma ksenobiotikov i protsessy perekisnogo okisleniia lipidov v mikrosomakh pecheni krys.