Growth of 293 cells in suspension culture.
A subline of 293 cells able to grow in suspension culture has been developed by passage of 293 cells through nude mice. This new line, designated 293N3S, grows with a doubling time of approximately 30 h, continues to express adenovirus 5 early region 1 (E1) antigens, and remains permissive for adenovirus 5 host range mutants defective in E1 functions.
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D046508:Culture Techniques / Q000379:methods; D004622:Embryo, Mammalian; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D000951:Antigens, Neoplasm