title: Morphogenesis of the elastic fiber: an immunoelectronmicroscopy investigation.
abstract: In this study a rabbit antiserum against human aortic elastin, which showed a high degree of species specificity in ELISA tests, was used to examine elastin fiber formation in the human fetal aorta between the ages of 14 and 23 weeks. Elastin was first detected by the antibody in the matrix of the 14-week-old specimen in association with the microfibrillar component. At this stage of development, the sections did not reveal structures morphologically identifiable as elastin. By the 17th week, discrete loci of elastin deposition were observed together with well-defined elastin fibrils. Only by the 23rd week did the aorta show the characteristic layering of elastic fibrils separating the myoblasts of the tunica media. In the latter specimen, the newly synthesized uncrosslinked elastin appeared to be unevenly distributed on the surface of elastin fibrils where it formed continuous strips of variable width arranged mostly in the form of spirals. This observation is discussed with respect to the proposals that the morphogenesis of elastic tissue is a dynamic process involving a close interrelationship between elastic fibrils and elastogenic cells and the morphogenetic movement of elastogenic cells plays an important role not only in the growth of elastic fibrils but also in the ultrastructural organization of the tissue.
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D001011:Aorta / Q000196:embryology / Q000648:ultrastructure; D002417:Cattle; D004549:Elastin / Q000378:metabolism*; D005333:Fetus / Q000502:physiology / Q000648:ultrastructure; D005865:Gestational Age; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008022:Ligaments / Q000648:ultrastructure; D008854:Microscopy, Electron; D011817:Rabbits / Q000276:immunology
chemical_list: D007106:Immune Sera; D004549:Elastin