title: Protective mechanisms against pulmonary infection with influenza virus. I. Relative contribution of polymorphonuclear leukocytes and of alveolar macrophages to protection during the early phase of intranasal infection.
abstract: The relative contribution of polymorphonuclear leukocytes and macrophages in the early protection against intranasal infection of mice with influenza virus was investigated. Virus multiplication in the lung in the early phase of infection with less than 1.5 X 10(3) plaque-forming units was enhanced by X-ray irradiation. The intranasal administration of carrageenan did not influence the titre of virus. However, when mice were infected with 1.5 X 10(4) plaque-forming units, the virus titre was elevated by intranasal administration of carrageenan as well as by X-ray irradiation, but not by intraperitoneal administration of carrageenan. The intranasal administration of carrageenan not only inhibited the phagocytic activity of alveolar macrophages but also enhanced susceptibility to the virus. On the other hand, polymorphonuclear leukocytes were capable of phagocytosing the virus in vitro and were non-permissive for virus infection. Neutralizing antibody and interferon were not detectable in the early stage of the infection. These results suggested that polymorphonuclear leukocytes (X-ray-sensitive, carrageenan-resistant) were the cells primarily responsible for early protection in influenza virus infection and that after infection with a high dose of the virus alveolar macrophages (X-ray-resistant, carrageenan-sensitive) also played a protective role in the early phase.
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D005720:Gamma Rays; D009980:Influenza A virus / Q000302:isolation & purification; D008163:Luminescent Measurements; D008168:Lung / Q000382:microbiology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D009504:Neutrophils / Q000502:physiology*; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects / Q000528:radiation effects; D011650:Pulmonary Alveoli / Q000473:pathology
chemical_list: D002351:Carrageenan