[Isolation of Ca/Mg-dependent endonuclease of cell nuclei as the basic component in the chromatin nuclease complex of human lymphocytes].
Highly purified preparations of Ca/Mg-dependent cell nuclear endonuclease have been obtained from human spleen lymphocytes. The purification was 2000-fold, and the enzyme was a polypeptide with a molecular weight of 57-54 kD. The study of its binding with monoclonal antibody enzyme, produced by various hybridoma strains obtained upon the immunization of mice with non-fractionated nuclear extract has shown that Ca/Mg-dependent endonuclease was the best expressed endonuclease of lymphocyte cellular nuclei. Possible production of some other cell nuclear endonucleases during processing of CH/Mg-dependent endonuclease is suggested.
D000911:Antibodies, Monoclonal / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology*; D002843:Chromatin / Q000201:enzymology*; D004706:Endodeoxyribonucleases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology*; D008722:Methods; D013154:Spleen / Q000201:enzymology
D000911:Antibodies, Monoclonal; D002843:Chromatin; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Vydelenie Ca/Mg-zavisimoĭ éndonukleazy kletochnykh iader kak osnovnogo komponenta nukleaznofo kompleksa khromatina limfotsitov cheloveka.