title: Tumor type-specific differences in cell-substrate adhesion among human tumor cell lines.
abstract: Cell lines derived from human tumors of 4 different histological types (squamous carcinomas, melanomas, gliomas and a fibrosarcoma) were examined for cell-substrate adhesion on plastic culture dishes and dishes coated with 50 micrograms of type-IV collagen. In the absence of exogenous adhesion factors, the squamous carcinoma cells attached and spread more rapidly than the other cells on both substrates. Once attached, the squamous carcinoma cells were also more difficult than the other cells to remove with proteolytic enzymes/EDTA. While the cell lines derived from melanomas, gliomas and the fibrosarcoma were less adhesive than the squamous carcinoma lines in the absence of exogenous adhesion factors, these cells were highly responsive to laminin. In contrast, laminin only slightly enhanced the attachment and spreading of squamous carcinoma cells on the plastic dishes and actually inhibited attachment and spreading on the collagen-coated dishes. These results indicate that there are tumor-type-specific differences in adhesiveness among human tumor cell lines and that cells from different tumor types may have distinct mechanisms for carrying out one of the functions critical to invasion.
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002448:Cell Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D005353:Fibronectins / Q000494:pharmacology; D005354:Fibrosarcoma / Q000473:pathology; D005910:Glioma / Q000473:pathology; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D008545:Melanoma / Q000473:pathology; D009369:Neoplasms / Q000473:pathology*; D019699:Thrombospondins; D013997:Time Factors
chemical_list: D005353:Fibronectins; D006023:Glycoproteins; D007797:Laminin; D019699:Thrombospondins