N-hydroxyaminofluorene: a chemical probe for DNA conformation.
The importance of the polymorphism of DNA in the reaction with the chemical carcinogen N-hydroxyaminofluorene is studied by means of a supercoiled plasmid containing an insert of (dC-dG). Immunochemical titration and the determination of the binding spectrum of -aminofluorene adducts show that the carcinogen reacts with B-DNA but not with Z-DNA and that conformational changes of the B-DNA-Z-DNA junctions occur as a function of the superhelical density.
D002273:Carcinogens; D004247:DNA; D004278:DNA, Superhelical; D005449:Fluorenes; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011110:Polymorphism, Genetic
D002273:Carcinogens; D004278:DNA, Superhelical; D005449:Fluorenes; C001368:N-hydroxy-2-aminofluorene; D004247:DNA