A modification of the method for the measurement of cerebral blood flow using [14C]iodoantipyrine in small animals.
We have modified the method for the measurement of cerebral blood flow using [14C]iodoantipyrine in small animals to overcome problems caused by rapid sequential arterial sampling. A second isotope, gamma-emitting [99mTc]pertechnetate, is infused together with [14C]iodoantipyrine. The 99mTc is counted externally by a detector placed over the chest. The arterial curve for [14C]iodoantipyrine can be reconstructed from a single arterial sample collected from the severed neck and the shape of the 99mTc chest curve. Because of the short half-life of 99mTc (6 h), all 14C measurements, including preparation of autoradiograms, may be made 3 days later.
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives*; D002250:Carbon Radioisotopes; D002560:Cerebrovascular Circulation; D051381:Rats; D013667:Technetium
D002250:Carbon Radioisotopes; D013667:Technetium; D000983:Antipyrine; C008135:iodoantipyrine