Morphometric and topologic analysis of freeze-fractured interphase nuclei.
Interphase rat liver nuclei were studied by freeze fracturing followed by electron microscopic observations. This method permits information on the native organization of the nuclear components in the hydrated state to be obtained. Morphometric analyses, performed with a Leitz Texture Analysis System, gave precise information on the different nuclear components, based on the histograms of their size distribution in heterochromatin, interchromatin and nucleolar areas. The textural characteristics were analyzed by computer to determine the topologic distribution of the solenoid chromatin fibers, the nucleosome filaments and the ribonucleoproteins in the different nuclear domains.
D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D005614:Freeze Fracturing; D006570:Heterochromatin / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D007399:Interphase; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000648:ultrastructure; D051381:Rats
D006570:Heterochromatin