title: [Bilirubin binding to glycosylated human serum albumin].
abstract: Glycosylation of human blood serum albumin was carried out by means of prolonged incubation of the protein with an excess of D-glucose or D-glucose-6-phosphate. The amount of glucose covalently bound to the protein was determined using thiobarbituric acid or pyridoxal-5-phosphate. The binding of bilirubin to glycosylated albumin was decreased with an increase in amount of glucose incorporated into the macro-molecule.
mesh_terms: D001663:Bilirubin / Q000097:blood*; D001665:Binding Sites; D001786:Blood Glucose / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001786:Blood Glucose; D012709:Serum Albumin; D001663:Bilirubin
vernacular_title: Sviazyvanie bilirubina s glikozilirovannym syvorotochnym al'buminom cheloveka.