Testosterone-induced DNA synthesis in cultured rat ventral prostate: effects of estracyt and its derivatives.
Testosterone-induced DNA synthesis in cultured rat ventral prostate was used to compare the direct effects of Estracyt and Emcyt with that of their metabolite, estramustine, and their carrier-hormone, oestradiol-17 beta on prostatic growth. In serum-supplemented medium (5-20% FCS), all the compounds were equally effective in suppressing testosterone stimulated DNA synthesis which was reduced by between 40-50%, whereas in serum-free medium the estramustine compounds were consistently less effective than oestradiol-17 beta. In the presence of 4 X 10(-9) M testosterone in serum-free medium, stimulated DNA synthesis was reduced by 15-30% following incubation with 4 X 10(-7) M of Estracyt, Emcyt and estramustine and by 60% with 4 X 10(-7) M oestradiol-17 beta. Thus, none of the estramustine compounds appear to offer any selective advantage over that of oestradiol-17 beta in suppressing prostatic DNA synthesis at the target tissue level.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D004961:Estramustine / Q000031:analogs & derivatives / Q000494:pharmacology*; D007065:Idoxuridine / Q000378:metabolism; D008297:Male; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*; D009924:Organ Culture Techniques; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000494:pharmacology*
D009588:Nitrogen Mustard Compounds; D004961:Estramustine; D013739:Testosterone; D004958:Estradiol; D004247:DNA; D007065:Idoxuridine