Tropomyosin from human erythrocyte membrane polymerizes poorly but binds F-actin effectively in the presence and absence of spectrin.
Actin in the human erythrocyte forms short protofilaments which are only long enough to accommodate tropomyosin monomers (Shen, B.W., Josephs, R. and Steck, T.L. (1986) J. Cell Biol. 102, 997-1006). This interaction between actin and tropomyosin monomers is predicted to be weak, since tropomyosin polymerization parallels its affinity for F-actin. We examine the binding of human erythrocyte tropomyosin to actin in the presence and absence of spectrin and its ability to polymerize. The binding of human erythrocyte tropomyosin to F-actin is not affected appreciably by the present of spectrin. Saturating F-actin with erythrocyte tropomyosin, however, weakens the binding of spectrin dimers to actin. Although tropomyosin from human erythrocyte and rabbit cardiac muscle have similar affinity for F-actin, the polymerizability of erythrocyte tropomyosin as determined by viscosity measurements is much reduced relative to muscle tropomyosin. This unusual property of erythrocyte tropomyosin is likely due to differences in its primary structure from other known tropomyosin at the amino and carboxyl terminal regions which are responsible for its head-to-tail polymerization and cooperative binding to F-actin. Analysis of the distribution of tyrosine by 2-dimensional tryptic mapping of 125I-labelled erythrocyte tropomyosin shows that tyrosine at positions 162, 214, 221, 261 and 267 in rabbit cardiac tropomyosin are conserved in human erythrocyte tropomyosin but Tyr-60 is absent. This observation suggests that erythrocyte tropomyosin has a carboxyl terminal region similar to its muscle counterparts but its amino terminal region resembles that of platelet tropomyosin which also lacks Tyr-60.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006736:Horses; D006801:Humans; D009206:Myocardium / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011108:Polymers / Q000096:biosynthesis*; D011817:Rabbits; D013049:Spectrin / Q000378:metabolism / Q000494:pharmacology*; D014335:Tropomyosin / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D014443:Tyrosine / Q000097:blood / Q000378:metabolism; D014783:Viscosity
D000199:Actins; D010455:Peptides; D011108:Polymers; D014335:Tropomyosin; D013049:Spectrin; D014443:Tyrosine