Evidence for the role of fibronectin in amphibian gastrulation.
In amphibian embryos, fibronectin (FN) assembles as a fibrillar network on the roof of the blastocoel cavity, preceding mesodermal cell migration. Local inversion of the ectoderm to produce a site where no FN is available prevents mesodermal cell migration. Microinjection of monovalent antibodies to FN arrests gastrulation. A complete inhibition of mesodermal cell migration is obtained after microinjection of a synthetic peptide containing the cell binding site sequence of FN. Prevention of interactions between receptors and FN appears to be the primary cause for blockage of gastrulation.
D000558:Ambystoma mexicanum; D000663:Amphibians / Q000196:embryology* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002465:Cell Movement; D004625:Embryo, Nonmammalian / Q000648:ultrastructure; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism*; D005775:Gastrula / Q000378:metabolism*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010999:Pleurodeles
D000906:Antibodies; D005353:Fibronectins