Anisotropy decay of diphenylhexatriene in melittin-phospholipid complexes by multifrequency phase-modulation fluorometry.
Frequency-domain fluorometry was used to investigate the intensity and anisotropy decays of diphenylhexatriene (DPH) in melittin-lipid complexes. Simulated and experimental data indicate that correlation times ranging from 0.3 to 500 ns can be determined using data from 1 to 200 MHz. For the melittin-lipid complexes the hindered rotator model was not adequate to account for the anisotropy decays, especially at temperatures above the transition temperatures. At high protein-to-lipid ratios the data revealed the formation of small particles (100 A) of melittin and dipalmitoylphosphatidylcholine and the disruption of membrane order in bilayers of dipalmitoylphosphatidic acid.
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism; D001514:Bee Venoms / Q000378:metabolism*; D004161:Diphenylhexatriene / Q000378:metabolism*; D005453:Fluorescence; D005470:Fluorometry; D008051:Lipid Bilayers / Q000378:metabolism; D008555:Melitten / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011090:Polyenes / Q000378:metabolism*
D001514:Bee Venoms; D008051:Lipid Bilayers; D010743:Phospholipids; D011090:Polyenes; D004161:Diphenylhexatriene; D008555:Melitten; D015060:1,2-Dipalmitoylphosphatidylcholine