Determination of 25-hydroxyvitamin D3 in sheep tissues by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method is described for the determination of 25-hydroxyvitamin D3 (25-OH-D3) in sheep tissue. The procedure involved saponification followed by chromatography on Sephadex LH-20 in order to remove extraneous material. Quantitation of 25-OH-D3 in tissue samples containing as little as 2 ng/g, was achieved by HPLC and ultraviolet detection at 254 nm in HPLC system.
D000818:Animals; D002112:Calcifediol / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet; D014018:Tissue Distribution
D002112:Calcifediol