title: [Study of the effect of phosphorylation of histones on their interaction with DNA-cellulose].
abstract: Data about the influence of phosphorylation levels of histones isolated from the prereplicative and replicative phases induced to proliferation by cycloheximide of rat hepatocytes, on their interaction with DNA-cellulose are presented. It is shown that the DNA-cellulose chromatography of histones is a sensitive model for the investigation of the effect of phosphorylation of histones on their electrostatic interaction with DNA. Using this method it is possible to separate high-phosphorylated subtype of rat liver H1 histones from other subtypes and also to show a direct dependence between the phosphorylation levels of histones and stability of their binding with DNA in prereplicative and replicative phases not only under conditions of proliferation induction by cycloheximide, but also under the subsequent influence of X-irradiation and serotonin.
mesh_terms: D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives*; D002852:Chromatography, Ion Exchange; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000031:analogs & derivatives*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D012701:Serotonin / Q000494:pharmacology
chemical_list: C033190:DNA-cellulose; D006657:Histones; D012701:Serotonin; D002482:Cellulose; D004247:DNA; D003513:Cycloheximide
vernacular_title: Issledovanie vliianiia fosforilirovaniia gistonov na ikh vzaimodeĭstvie s DNK-tselliulozoĭ.