title: [Interaction of NAD(H)-dependent dehydrogenases with active dyes and their complexes with transitional metal ions].
abstract: The interaction of NAD(H)-dependent dehydrogenases--yeast alcohol dehydrogenase and rabbit muscle lactate dehydrogenase--with reactive dyes produced in the USSR was studied. The essential role of metal ions in specific binding of alcohol dehydrogenase and dyes was demonstrated by differential spectroscopy, circular dichroism spectroscopy and chromatography. Lactate dehydrogenase in contrast with alcohol dehydrogenase does not require metal ions for the binding of the above-said dyes. A comparative study of eluting abilities of selected desorption agents (imidazole, adenine, 8-oxyquinoline-5-sulfonic acid, NAD, AMP, EDTA) by alcohol dehydrogenase chromatography on adsorbents with light-resistant yellow 2KT-Cu(II) and orange 5K revealed the differences in competition of the dyes for NAD-binding sites of alcohol dehydrogenase. The participation of light-resistant yellow 2KT-Cu(II) in the formation of mixed complexes with imidazole, adenine, 8-oxyquinoline-5-sulfonic acid, NAD and EDTA suggests that the specific binding of alcohol dehydrogenase to light-resistant yellow 2KT-Cu(II) is due to coordination between the Cu(II) ion and the amino acid residue in alcohol dehydrogenase.
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D004396:Coloring Agents; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008024:Ligands; D009132:Muscles / Q000201:enzymology; D011817:Rabbits; D015003:Yeasts / Q000201:enzymology
chemical_list: D004396:Coloring Agents; D008024:Ligands; D003300:Copper; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase
vernacular_title: Issledovanie vzaimodeĭstviia NAD(H)-zavisimykh degidrogenaz s aktivnymi krasiteliami i ikh kompleksami s ionami perekhodnykh metallov.