Utilization of porcine pancreatic phospholipase A2 for the preparation of a marine fish oil enriched in (n - 3) polyunsaturated fatty acids.
A simple and rapid method is described for the preparation of a marine oil fraction highly enriched in (n - 3) polyunsaturated fatty acids. Cod roe, containing lipid up to 15% of its dry weight, approximately 70% of which is phospholipid, was the starting material. Incubation of a concentrated aqueous extract of the roe with porcine pancreatic phospholipase A2 (EC 3.1.1.4) was the key step in the procedure. Extraction of the freeze-dried reaction product with diethyl ether containing formic acid produced an oil in a yield of 1.0 g/100 g wet wt of starting roe. The oil contained over 95% as free fatty acids, with 20:5 (n - 3) and 22:6 (n - 3) accounting for up to 24 and 40%, respectively, of the total free fatty acids. The therapeutic use of the oil is mentioned.
D000818:Animals; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification*; D005395:Fish Oils / Q000032:analysis*; D005612:Freeze Drying; D006868:Hydrolysis; D010179:Pancreas / Q000201:enzymology*; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D013552:Swine
D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2