title: Enteroglucagon and experimental intestinal carcinogenesis in the rat.
abstract: To assess the association between the putative intestinal trophic hormone enteroglucagon and the development of intestinal tumours, four groups of 20 rats underwent either jejunal transection or 20%, 50%, or 80% proximal small bowel resection. Tumours were induced with azoxymethane 10 mg/kg weekly for 12 weeks. At 26 weeks there was a promotion of colonic neoplasia from a median of 0.5 (range 0-3) per rat in the transection group to 1.0 (0-3) in the 50% resected group (p less than 0.01) but no significant promotion in the 80% resection group. In the small bowel, increasing resection resulted in a progressive promotion of tumours from a median of 1.0 (range 0-3) per rat in the transection group to 2.0 (0-5) in the 50% resection group (p less than 0.001) and 3.0 (0-11) in the 80% group (p less than 0.01). Plasma enteroglucagon was measured at 2, 16, and 26 weeks and was raised seven-fold in the 80% resected group (p less than 0.001). There was a significant correlation between enteroglucagon concentrations and number of duodenal tumours but not colonic tumours. Crypt cell production rate in the duodenum increased from 11.5 +/- 1.9 to 29.2 +/- 1.4 cells/crypt/h in the 80% resected group (p less than 0.001) and showed a close correlation with both enteroglucagon levels and tumour promotion in the small bowel. There were no changes in crypt cell production rate in the colon with resection. This study shows a close association between enteroglucagon concentrations, promotion of tumours and crypt cell production rate in the duodenum but not in the colon.
mesh_terms: D000818:Animals; D001397:Azoxymethane; D003110:Colonic Neoplasms / Q000097:blood; D004379:Duodenal Neoplasms / Q000097:blood; D005768:Gastrointestinal Hormones / Q000097:blood*; D004763:Glucagon-Like Peptides / Q000097:blood*; D007414:Intestinal Neoplasms / Q000097:blood* / Q000139:chemically induced; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005768:Gastrointestinal Hormones; D004763:Glucagon-Like Peptides; D001397:Azoxymethane