Terminal structure of hypovirulence-associated dsRNAs in the chestnut blight fungus Endothia parasitica.
The 3'- and 5'-terminal sequences of the five large double-stranded RNA species (L-dsRNA; 4.5-6.0 X 10(6) daltons) of EP713, a hypovirulent strain of Endothia parasitica, were determined by mobility-shift and enzymatic methods. All the L-dsRNAs appeared to have identical terminal sequences. A heteropolymer sequence was found at one 3'-terminus and a poly(A) sequence of variable length at the other. It was possible to label only one 5'-terminus using polynucleotide kinase and [gamma-32P]ATP, and this was shown to be a poly(U) sequence of variable length. We propose that the dsRNAs have the following structure, where X represents a blocking group: (Formula: see text). A recombinant plasmid containing dsRNA-related sequences was constructed. Hybridization analysis using the recombinant probe indicated that the sequence homology among the L-dsRNAs extended beyond these terminal regions and was also shared by small dsRNAs (0.3-0.45 X 10(6) daltons).
D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004274:DNA, Recombinant / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010935:Plant Diseases; D010957:Plasmids; D012330:RNA, Double-Stranded / Q000235:genetics*; D014774:Virulence; D013098:Xylariales / Q000235:genetics* / Q000472:pathogenicity
D004274:DNA, Recombinant; D012330:RNA, Double-Stranded; D004247:DNA