Specificity of anti-polynucleotide monoclonal antibodies from human-human hybridomas.
Nine human-human hybridoma clones, secreting monoclonal antibodies reactive with nucleic acids, were generated by fusing with lymphocytes of lung cancer or systemic lupus erythematosus patients. These hybridoma antibodies were classified into 5 types, in terms of reactivities with DNA, RNA, various synthetic nucleic acids and cardiolipin. Hybridoma clone SU-1 secreted antibody reacting with dsDNA, ssDNA and RNA (type I). Clone HL-321 did not react with these, but with poly (dT), poly (I) and poly (G) (type II). Clone HL-349 was reactive with almost all nucleic acids tested and also with cardiolipin (type III). Clones HF-4, HF-7, HB-7 and HL-259 reacted with ssDNA, poly (A), poly (G) and cardiolipin, but not with RNA (type IV). HB-5 and SH-9 antibodies were reactive only with poly (dT) (type V).
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002308:Cardiolipins / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D008175:Lung Neoplasms; D008180:Lupus Erythematosus, Systemic; D008214:Lymphocytes; D011119:Polynucleotides / Q000276:immunology*
D000911:Antibodies, Monoclonal; D002308:Cardiolipins; D011119:Polynucleotides