Cell cycle regulated synthesis of stable mouse thymidine kinase mRNA is mediated by a sequence within the cDNA.
The cDNA for mouse thymidine kinase (TK) was isolated from a cDNA library in lambda-gt11 and sequenced. It was used as a probe to follow the time course of TK mRNA expression in growth stimulated mouse fibroblasts. Linked to the HSV-TK promoter the cDNA was able to transform LTK-cells to the TK+ phenotype. The transformed cells expressed the TK mRNA and enzyme activity in a growth dependent fashion suggesting that the regulatory element is localized on the cDNA.
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D004247:DNA / Q000378:metabolism*; D004622:Embryo, Mammalian; D007739:L Cells / Q000201:enzymology; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D013937:Thymidine Kinase / Q000172:deficiency / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA; D013937:Thymidine Kinase