title: Prediction of the halothane (Hal) genotypes of pigs by deducing Hal, Phi, Po2, Pgd haplotypes of parents and offspring: results from a large-scale practice in Swedish breeds.
abstract: Results from a large-scale study, comprising 75 different breeding herds, are reported on predicting the halothane (Hal) genotypes of individual pigs by making use of the known close linkage between Hal and three electrophoretic blood marker loci (Phi, Po2, Pgd). The parents haplotypes (involving Hal and marker loci) were determined from the HAL phenotypes (halothane test results) and marker loci phenotypes of their offspring in the first one or two litters studied. In subsequent litters of the Hal-marker loci haplotyped parents, the offspring's expected Hal genotypes could be predicted on the basis of the marker loci haplotypes inherited by them. By comparing the expected and observed HAL phenotypes of offspring in subsequent litters, the predicted Hal genotype was found to be correct in 90-95% of the 4000 offspring (from Nn X Nn and Nn X nn matings) of Swedish Landrace and Yorkshire breeds studied. The order of the three marker loci was confirmed as Phi-Po2-Pgd but the position of Hal with regards to Phi could not be resolved. The recombination frequencies between the most distant loci in this region, viz. Hal-Pgd and Phi-Pgd, were estimated to be 3-4.5% and 4-6%, respectively. The easy and rapid electrophoretic techniques described in the study to phenotype PHI, PO2, PGD, also allowed phenotyping of six other polymorphic protein systems on the same gels. Thus Hal genotyping and effective parentage control can be conducted simultaneously.
mesh_terms: D000483:Alleles; D000818:Animals; D001798:Blood Proteins / Q000235:genetics / Q000302:isolation & purification; D003433:Crosses, Genetic; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D006221:Halothane / Q000009:adverse effects*; D008297:Male; D008305:Malignant Hyperthermia; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics; D013548:Sweden; D013552:Swine / Q000235:genetics*
chemical_list: D001798:Blood Proteins; D010734:Phosphogluconate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase; D006221:Halothane