A regulatory polymorphism for rat hepatic cytochrome P-450g.
Rat hepatic cytochrome P-450g is a male-specific hemoprotein found at significant levels only in adult animals. In the present study, two-dimensional gel electrophoretic and immunochemical methods were used to study a polymorphism of this isozyme and its ontogenetic regulation. Inbred ACI/Hsd and WF/Hsd rats were found to express high and low levels of cytochrome P-450g, respectively. F1 hybrids of these strains showed additive inheritance for this trait and the responsible gene was found to be autosomal. Cytochrome P-450g and another male-specific form of the enzyme, cytochrome P-450h, were characterized by a similar time-course for their ontogenetic expressions. However, unlike cytochrome P-450g, the level of cytochrome P-450h was indistinguishable in hepatic microsomes from mature ACI/Hsd and WF/Hsd rats. Considering these results, we tentatively conclude that the gene regulating the level of cytochrome P-450g is Cis-acting.
D000375:Aging; D000818:Animals; D003101:Collodion; D003433:Crosses, Genetic; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005809:Genes, Regulator; D007159:Immunologic Tests; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D011110:Polymorphism, Genetic; D051381:Rats; D011912:Rats, Inbred ACI; D011920:Rats, Inbred WF
D007527:Isoenzymes; D003101:Collodion; D003577:Cytochrome P-450 Enzyme System