Enteroglucagon and experimental intestinal carcinogenesis in the rat.
To assess the association between the putative intestinal trophic hormone enteroglucagon and the development of intestinal tumours, four groups of 20 rats underwent either jejunal transection or 20%, 50%, or 80% proximal small bowel resection. Tumours were induced with azoxymethane 10 mg/kg weekly for 12 weeks. At 26 weeks there was a promotion of colonic neoplasia from a median of 0.5 (range 0-3) per rat in the transection group to 1.0 (0-3) in the 50% resected group (p less than 0.01) but no significant promotion in the 80% resection group. In the small bowel, increasing resection resulted in a progressive promotion of tumours from a median of 1.0 (range 0-3) per rat in the transection group to 2.0 (0-5) in the 50% resection group (p less than 0.001) and 3.0 (0-11) in the 80% group (p less than 0.01). Plasma enteroglucagon was measured at 2, 16, and 26 weeks and was raised seven-fold in the 80% resected group (p less than 0.001). There was a significant correlation between enteroglucagon concentrations and number of duodenal tumours but not colonic tumours. Crypt cell production rate in the duodenum increased from 11.5 +/- 1.9 to 29.2 +/- 1.4 cells/crypt/h in the 80% resected group (p less than 0.001) and showed a close correlation with both enteroglucagon levels and tumour promotion in the small bowel. There were no changes in crypt cell production rate in the colon with resection. This study shows a close association between enteroglucagon concentrations, promotion of tumours and crypt cell production rate in the duodenum but not in the colon.
D000818:Animals; D001397:Azoxymethane; D003110:Colonic Neoplasms / Q000097:blood; D004379:Duodenal Neoplasms / Q000097:blood; D005768:Gastrointestinal Hormones / Q000097:blood*; D004763:Glucagon-Like Peptides / Q000097:blood*; D007414:Intestinal Neoplasms / Q000097:blood* / Q000139:chemically induced; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D005768:Gastrointestinal Hormones; D004763:Glucagon-Like Peptides; D001397:Azoxymethane