Membrane penetration of bovine factor V and Va detected by labeling with 5-iodonaphthalene-1-azide.
The membrane-binding properties of Factor V and Factor Va were investigated using the lipophyllic, photoactivable probe 5-[125I]iodonaphthalene-1-azide. In the presence of vesicles composed of 75% phosphatidylcholine and 25% phosphatidylserine, both Factor V and Va were found to be labeled by the probe. The label was almost exclusively localized to the carboxyl-terminal-derived component E of Factor Va. The results are consistent with the interpretation that component E is the membrane binding subunit of Factor Va and that the interaction between Factor V or Factor Va and the membrane involves the penetration of the protein into the lipid bilayer.
D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D005165:Factor V / Q000378:metabolism*; D015943:Factor Va; D008051:Lipid Bilayers / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D008970:Molecular Weight; D010713:Phosphatidylcholines; D010718:Phosphatidylserines / Q000378:metabolism; D010777:Photochemistry
D000345:Affinity Labels; D001386:Azides; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; C015298:iodonaphthylazide; D015943:Factor Va; D005165:Factor V; D002118:Calcium