title: Comparison of two methods for determination of magnesium concentration of mononuclear blood cells.
abstract: We describe and compare two methods for the determination of magnesium concentration in lymphocytes from cell cultures. The magnesium concentration of lymphocytes was 36 +/- 4 mmol/kg dry weight by atomic absorption spectroscopy analysis and 39 +/- 19 mmol/kg dry weight with electron probe X-ray microanalysis. We found no significant difference between the results for the two methods. The coefficient of variation was 30% for magnesium concentration among individual cells with electron probe X-ray microanalysis.
mesh_terms: D002051:Burkitt Lymphoma / Q000032:analysis; D002478:Cells, Cultured; D004577:Electron Probe Microanalysis / Q000379:methods; D006801:Humans; D008214:Lymphocytes / Q000032:analysis*; D008274:Magnesium / Q000097:blood*; D013054:Spectrophotometry, Atomic / Q000379:methods
chemical_list: D008274:Magnesium