Immunoblotting detection of lectins in gluten and white rice flour.
The gluten lectin was isolated by affinity chromatography, separated by sodium dodecyl sulphate-gel electrophoresis together with purified wheat germ agglutinin (WGA) and electrotransferred to nitrocellulose filters. The binding pattern of anti-WGA to the blotted filters confirmed the presence of WGA in gluten. A lectin from rice bran and white rice flour, respectively, was isolated by affinity chromatography. Both lectins reacted with anti-WA in immunoblotting. As patients with coeliac disease are known to tolerate rice flour, the finding of a WGA-like lectin questioned the suggestion that WGA in gluten is involved in the pathogenesis of coeliac disease. A second lectin was also isolated from rice flour which reacted only with antibodies against soybean lectin on immunoblots. This may indicate a contamination of soybean proteins in rice flour.
D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002446:Celiac Disease / Q000276:immunology; D002846:Chromatography, Affinity; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000032:analysis*; D006801:Humans; D007159:Immunologic Tests; D037102:Lectins / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D012275:Oryza / Q000032:analysis*; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D037102:Lectins; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; D005983:Glutens; D003101:Collodion