title: Glucocorticoids change the nucleotide and sugar nucleotide pool sizes in cultured human skin fibroblasts.
abstract: Fibroblasts in culture were treated with the glucocorticoid budesonide. The nucleotide and sugar nucleotide pools were quantitated after separation by isotachophoresis. Steroid treatment induced a 40% increase of the UDP-N-acetylhexosamine pool and a 30% increase of the UDP-glucuronic acid pool whereas the UTP pool was diminished. These effects became apparent after 24 h of incubation and a new steady state was attained after 48 h of incubation. The 3'-phosphoadenosine-5'-phosphosulfate pool was probably not influenced by the glucocorticoid treatment. The half-life of the UDP-N-acetylhexosamine pool was considerably longer in treated than in control cells. The efflux from the UDP-N-acetylhexosamine pool was also lowered in the treated cells. The changed efflux may be due to a decreased glycoconjugate synthesis induced by glucocorticoid treatment. The rate of equilibration of [14C]glucose and [3H]glucosamine with the UDP-N-acetylhexosamine pool was changed by glucocorticoid treatment; especially that of [3H]glucosamine was decreased.
mesh_terms: D019819:Budesonide; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005938:Glucocorticoids / Q000494:pharmacology*; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D009711:Nucleotides / Q000378:metabolism*; D011282:Pregnenediones / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D014544:Uridine Triphosphate / Q000378:metabolism
chemical_list: D005938:Glucocorticoids; D009711:Nucleotides; D011282:Pregnenediones; D013431:Sulfates; D014535:Uridine Diphosphate Glucuronic Acid; D019819:Budesonide; D014537:Uridine Diphosphate N-Acetylglucosamine; D014536:Uridine Diphosphate N-Acetylgalactosamine; D005947:Glucose; D005944:Glucosamine; D014544:Uridine Triphosphate