[Effect of ionizing radiation and Fe2+-induced peroxidation on the lipid phase of erythrocyte membrane preparations].
The fluorescent probes, perilene and diphenyl hexatriene, were used to study changes in the lipid phase of erythrocytic ghosts induced by ionizing radiation (100-1000 Gy) and lipid peroxidation initiated by Fe2+ (5-100 microM). Both of the factors were shown to bring about similar changes in the membrane, that is, an increase in the viscosity of the probe localization sites and a decrease in diphenyl hexatriene fluorescence intensity. During the postirradiation incubation of the exposed membranes they were additionally damaged whereas upon peroxidation, most of the changes occurred after 15-min incubation with Fe2+.
D000818:Animals; D004161:Diphenylhexatriene / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005296:Ferrous Compounds / Q000494:pharmacology*; D005456:Fluorescent Dyes; D005720:Gamma Rays; D008054:Lipid Peroxides / Q000097:blood* / Q000528:radiation effects; D008563:Membrane Lipids / Q000097:blood / Q000528:radiation effects*; D010084:Oxidation-Reduction; D010569:Perylene / Q000494:pharmacology; D051381:Rats; D013997:Time Factors; D014783:Viscosity
D005296:Ferrous Compounds; D005456:Fluorescent Dyes; D008054:Lipid Peroxides; D008563:Membrane Lipids; D004161:Diphenylhexatriene; D010569:Perylene
Vliianie ioniziruiushcheĭ radiatsii i perekisnogo okisleniia, indutsiirovannogo Fe2+, na lipidnuiu fazu preparatov éritrotsitarnykh membran.