Correlation of urokinase activity from biopotency and high-performance liquid chromatographic assays.
A simpler, less expensive, and faster high-performance liquid chromatographic method was shown to be an alternative to urokinase potency determinations by the Ploug method. Post-elution recovery of the low-molecular-weight form was 104 +/- 2.4% as determined by the Ploug method. Two analysts reported relative standard deviations of 1.6% and 1.1% based on peak height determination of eight replicate injections of a single sample of low-molecular-weight material. Linearity at the same wavelength for low- and high-molecular-weight forms was 0.9999 and 0.9992, respectively, for peak height versus potency.
D002851:Chromatography, High Pressure Liquid; D004795:Enzyme Stability; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis*
D014568:Urokinase-Type Plasminogen Activator