Porcine parvovirus: propagation in microcarrier cell culture and immunogenic evaluation in pregnant gilts.
Porcine parvovirus was propagated in PK-15 cells cultured in roller bottles or on microcarrier beads. After inactivation, the virus was used as antigen in the preparation of vaccines. The immunogenic potency and safety of the vaccines were evaluated in specific pathogen free pregnant gilts and guinea pigs. Experimental challenge tests determined the efficacy of the vaccine in preventing porcine parvovirus transplacental infection. Neither viral antigens nor specific antibodies were detected in fetuses from vaccinated gilts. In contrast, fetal death and, or, mummification occurred when unvaccinated gilts were infected. Both virus and, or, antibodies were also detected in fetuses from these unvaccinated gilts. Serum conversion after vaccination was assayed by microserum neutralisation using guinea pig erythrocytes as cell indicators and by haemagglutination inhibition tests. Viral antigens in fetal tissues were detected using ELISA, the immunobeads technique, the haemagglutination test and by virus isolation.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D010321:Parvoviridae / Q000254:growth & development*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D013552:Swine / Q000382:microbiology*; D014776:Virus Cultivation / Q000379:methods