Computer-aided hybridoma screening. Production of monoclonal antibodies against membrane fractions from mammary tumour tissue.
Monoclonal antibodies were raised against membrane fractions from mammary tumour tissue. 746 hybridomas were obtained against the so-called membrane fraction '2a' and 1393 hybridomas against the so-called 'top-region'. These hybridomas were tested with an immunodot assay, cell-binding radioimmunoassay and an immunocytological screening assay using several antigens. In the evaluation of these data we used the common database software (dBASE II) on a personal computer. To facilitate computer handling, even for the non-specialist, we programmed our individual menu-driven software for computer-aided hybridoma screening.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000912:Antibodies, Neoplasm / Q000276:immunology / Q000302:isolation & purification*; D000951:Antigens, Neoplasm / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology*; D003201:Computers; D006825:Hybridomas / Q000032:analysis* / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology*; D008838:Microcomputers; D012984:Software
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm