[Determination of the lactate concentration in plasma using L-lactate:ferricytochrome c-oxidoreductase and tetraxolium salt].
A method is described for the determination of lactate in plasma using cytochrome b2, with tetrazolium salt as an electron acceptor. The chief advantages over the usual methods of lactate determination are high sensitivity, satisfactory specificity, high absorbance, use of the visible region of the spectrum, and low cost.
D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D042844:L-Lactate Dehydrogenase (Cytochrome); D007773:Lactates / Q000097:blood*; D013053:Spectrophotometry / Q000379:methods; D013778:Tetrazolium Salts
D007202:Indicators and Reagents; D007773:Lactates; D013778:Tetrazolium Salts; D007770:L-Lactate Dehydrogenase; D042844:L-Lactate Dehydrogenase (Cytochrome)
Bestimmung der Lactatkonzentration im Plasma mit L-Lactat:Ferricytochrom c-Oxidoreductase und Tetrazoliumsalz.