Rapid purification and properties of protein kinase C from bovine adrenal medulla.
Protein kinase C was purified from bovine adrenal medulla using a rapid procedure which resulted in an approximate 1500-fold increase in specific activity. The characteristics of the enzyme are reported and for the first time it is possible to compare the effects of TPA on secretion from intact and permeabilized cells with the effect of TPA on protein kinase C purified from the same secretory tissue.
D000313:Adrenal Medulla / Q000201:enzymology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002846:Chromatography, Affinity; D004789:Enzyme Activation / Q000187:drug effects; D010718:Phosphatidylserines; D011493:Protein Kinase C / Q000302:isolation & purification* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D010718:Phosphatidylserines; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium