Regulation of antibody production by hybridoma cultures. I. Anti-idiotype antibody-mediated down-regulation of anti-DNA antibody production by hybridoma cells.
A series of anti-DNA antibody-producing hybridomas were obtained by fusing spleen cells from 6-month-old MRL/lpr, autoimmune prone, mice with P3X63-Ag8 myeloma cells. Rabbit anti-idiotype (id) antibodies specific for several of the hybridoma proteins were prepared. It was shown that the anti-id antibody inhibited immunoglobulin in secretion by the hybridoma cells in an id-specific manner. Inhibition of antibody production was not due to a cytotoxic effect, since the anti-id, in fact, stimulated proliferation of the hybridoma cells.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D002455:Cell Division; D004247:DNA / Q000276:immunology; D005260:Female; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism*; D007130:Immunoglobulin Idiotypes / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D011817:Rabbits
D000906:Antibodies; D007130:Immunoglobulin Idiotypes; D004247:DNA