Identification of a protein in sera of normal humans that inhibits fibroblast chemotactic and random migration in vitro.
Normal human serum contains a 230,000 Mr protein that inhibits fibroblast chemotactic and random migration. This serum inhibitor of fibroblast migration (SIFM) is a heat-stable, trypsin-sensitive protein with a pI of 4.8 that reversibly inhibits the random and chemotactic migration of fibroblasts in vitro. Although SIFM effectively inhibits the chemotaxis of fibroblasts to interstitial collagens, fibronectin, lymphocyte-derived chemotactic factor for fibroblasts, and serum-derived chemotactic factor, it does not alter the chemotactic migration of human peripheral blood neutrophils or monocytes, and does not act as a cytotoxin to human dermal fibroblasts. The SIFM appears to act through a cell-directed mechanism to alter the fibroblast's ability to migrate. Serum inhibitor of fibroblast migration may function in vivo to modulate fibroblast migration under physiologic and pathologic conditions.
D001798:Blood Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects*; D003864:Depression, Chemical; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D009000:Monocytes / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D010413:Penis
D001798:Blood Proteins; D002630:Chemotactic Factors; C051350:fibroblast chemotactic inhibitor