ADP-ribosylation of a Mr 21,000 membrane protein by type D botulinum toxin.
When crude membrane fraction from bovine adrenal gland was incubated with type D botulinum toxin in the presence of NAD, a membrane protein with a molecular weight of 21,000 was specifically ADP-ribosylated. This ADP-ribosylation occurred dependent on the dose of the toxin and was abolished by prior boiling ADP-ribose transfer to the membrane protein was significantly suppressed when agmatine and L-arginine methyl ester were included in the reaction mixture. Dithiothreitol stimulated this ADP-ribosylation about 3-fold. Incubation of membrane fractions from mouse brain and pancreas with this toxin also resulted in ADP-ribosylation of a protein of the same molecular weight. These results suggested that type D botulinum toxin catalyzed transfer of an ADP-ribose moiety of NAD to the specific membrane protein common to secretory cells.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism; D000376:Agmatine / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001905:Botulinum Toxins / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002417:Cattle; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism
D008565:Membrane Proteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; C026512:arginine methyl ester; D000376:Agmatine; D001120:Arginine; D001905:Botulinum Toxins; D004229:Dithiothreitol