title: Distribution of bombesin-like peptides in the alimentary canal of several vertebrate species.
abstract: The objective of this study was to quantitate and characterize the variants of bombesin-like immunoreactivity in the alimentary canal of the rat, rabbit, hawk, owl, dog, monkey and human. Bombesin-like immunoreactivity was found throughout the entire gastrointestinal tract of all species studied. In the rat, the highest concentration of bombesin-like immunoreactivity was found in the colon. Gel chromatography showed that bombesin-like immunoreactivity corresponded to gastrin-releasing peptide (GRP-27) and GRP-10. In the dog, the greatest concentration of bombesin-like immunoreactivity was observed in the mucosal layer of the fundus, whereas the concentration of bombesin-like immunoreactivity in the muscle layer of the dog did not vary significantly from region to region. Gel chromatography showed that bombesin-like immunoreactivity in the dog corresponded to GRP-27, bombesin, GRP-10, and a smaller fragment. In the human, the concentration of bombesin-like immunoreactivity did not vary significantly from region to region in the mucosal and muscular layers. Gel chromatography of human fundal mucosa showed that bombesin-like immunoreactivity peaks occur in the regions of GRP-27, bombesin and GRP-10. These findings substantiate the observation that bombesin-like peptides play a variety of roles in the regulation of gut function.
mesh_terms: D000818:Animals; D001717:Birds; D001839:Bombesin / Q000032:analysis*; D002850:Chromatography, Gel; D003106:Colon / Q000032:analysis; D004064:Digestive System / Q000032:analysis*; D004285:Dogs; D005260:Female; D005748:Gastric Fundus / Q000032:analysis; D005753:Gastric Mucosa / Q000032:analysis; D019886:Gastrin-Releasing Peptide; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis; D008253:Macaca mulatta; D008254:Macaca nemestrina; D008297:Male; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D014018:Tissue Distribution
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D019886:Gastrin-Releasing Peptide; C040794:neuromedin C; D001839:Bombesin