Detection and enzymatic deglycosylation of a glycosylated variant of prolactin in human plasma.
Immunoperoxidase electrophoresis was applied to the plasma of a patient showing a high level of prolactin (PRL) secreted by a pituitary adenoma. Two PRL monomers were detected with an anti-hPRL antiserum: a major 22 kDa form and a minor 25 kDa form. Concanavalin A-Sepharose 4B chromatography revealed that the 25 kDa form was a glycosylated variant of PRL. Incubation of this variant with endoglycosidase F led to its transformation into the 22 kDa form.
D000236:Adenoma / Q000097:blood* / Q000378:metabolism; D006026:Glycoside Hydrolases; D006801:Humans; D010911:Pituitary Neoplasms / Q000097:blood* / Q000378:metabolism; D011388:Prolactin / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
C043928:prolactin, glycosylated; D011388:Prolactin; D006026:Glycoside Hydrolases