title: Control of gene expression in the P2-related template coliphages. III. DNA sequence of the major control region of phage 186.
abstract: The PstI fragment (65.5% to 77.4%) of coliphage 186, known genetically to encode the major control genes, has been sequenced, and an analysis performed to assess coding capacity, transcription-translation signals, and to identify any other significant features. Our analysis indicates that the region encodes: seven genes, including the int and cI genes, which overlap, the late control gene B, and two genes, named CP75 and CP76, encoding potential DNA-binding proteins; a promoter pB and terminator tB for the rightward transcription of the B gene, and we predict the existence of this transcript in a lysogen; a promoter pL and terminator tL for leftward transcription that encodes the int and cI genes, and represents the presumed lysogenic transcript; a promoter pR for rightward transcription to give the presumed (early) lytic transcript that is overlapping and convergent with the lysogenic transcript; and finally, a potential operator site for repressor binding in the region of the pR promoter. Preliminary evidence is presented to support this analysis.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral; D005786:Gene Expression Regulation; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012270:Ribosomes / Q000378:metabolism; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral