Accumulation of protoporphyrin-IX by the chlorophyll-less y-y mutant of Chlamydomonas reinhardtii.
A pigment accumulating in a Mendelian mutant (y-y) of Chlamydomonas reinhardtii, which has essentially no chlorophyll and lacks inner chloroplast membranes in the light and dark, was isolated and characterized. It was identified as protoporphyrin-IX (PROTO) by spectral analysis using two different methods of extraction and fractionation. The amount of PROTO was estimated to be 10(7) molecules per cell. Since PROTO was the only intermediate of chlorophyll biosynthesis that accumulated, we conclude the y-y lesion in the pathway is after PROTO.
D002696:Chlamydomonas / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000235:genetics / Q000378:metabolism*; D003624:Darkness; D008274:Magnesium / Q000378:metabolism; D009154:Mutation; D011166:Porphyrins / Q000378:metabolism*; D011524:Protoporphyrins / Q000378:metabolism*; D013057:Spectrum Analysis
D011166:Porphyrins; D011524:Protoporphyrins; D002734:Chlorophyll; C028025:protoporphyrin IX; D008274:Magnesium