[Cholesterol and phospholipid content of cells sensitive and resistant to ethidium bromide and its alteration in resistant cells as affected by methyltestosterone].
The content of cholesterol (Chl) and phospholipids (Phl) in cell lines L and CHO-K1, sensitive and resistant to the toxic action of ethidium bromide, has been studied. EB-resistant cells were shown to have lower amounts of Chl and Phl, and the ratio Chl/Phl in these cells was decreased too. EB-resistant cells, grown in serum-free medium, have dramatically decreased contents of Chl, while compared with the cells growing with serum. Treatment of EB-resistant cells with methyltestosterone in concentration of 3 X 10(-7) M caused the increase in Chl and Phl contents, thus approximating these indices to those of EB-sensitive ones.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects*; D002784:Cholesterol / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance; D004996:Ethidium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects / Q000378:metabolism; D008777:Methyltestosterone / Q000494:pharmacology*; D051379:Mice; D010743:Phospholipids / Q000378:metabolism*
D010743:Phospholipids; D002784:Cholesterol; D004996:Ethidium; D008777:Methyltestosterone
Soderzhanie kholesterina i fosfolipidov v kletkakh, chuvstvitel'nykh i ustoĭchivykh k bromistomu étidiiu, i ego izmenenie v ustoĭchivykh kletkakh pri deĭstvii metiltestosterona.