[Mass-spectrometric determination of the structure of glycopeptides from the bacterial cell wall (illustrated by Lactobacillus bulgaricus)].
Mass spectrometry has been applied to the structural analysis of one of the glycopeptides from blastolysin, antitumor bacterial preparation isolated from the Lactobacillus bulgaricus cell wall. The glycopeptide (MW 10,000) was subjected to partial acid hydrolysis (6 N HCl, 100 degrees C) and the resulting products were dansylated or trifluoroacetylated and methylated or deuteromethylated. The mixture of these derivatives was examined by high-performance liquid chromatography or gas chromatography followed by mass spectrometry using electron impact and ammonia chemical ionization techniques.
D000596:Amino Acids; D001426:Bacterial Proteins / Q000032:analysis*; D002473:Cell Wall / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D006020:Glycopeptides / Q000032:analysis*; D007778:Lactobacillus / Q000032:analysis*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis*
D000596:Amino Acids; D001426:Bacterial Proteins; D006020:Glycopeptides; D008565:Membrane Proteins
Mass-spektrometricheskoe usstanovlenie struktury glikopeptidov kletochnykh stenok bakteriĭ (na primere Lactobacillus bulgaricus).