Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation.
A method which allows for the quantitative measurement of DNA in plasma is described. After treatment of plasma with phenol, DNA is precipitated by ethanol using gelatin as a coprecipitating agent. DNA is then measured by nick translation labeling. This assay takes a few hours. It is suitable for the measurement of DNA within a range of 0.02 to 20 ng in 10 microliters of plasma. For example, it is applied to the measurement of DNA in plasma from mice injected with bacterial lipopolysaccharide.
D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000097:blood*; D005780:Gelatin; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008832:Microchemistry / Q000379:methods; D011092:Polyethylene Glycols; D013696:Temperature
D008070:Lipopolysaccharides; D011092:Polyethylene Glycols; D005780:Gelatin; D004247:DNA