Proliferative responses of snake lymphocytes to concanavalin A.
Spleen cells (SC) of adult snakes Psammophis sibilans responded to concanavalin A (Con A) in vitro by strong proliferation during the spring and autumn seasons. Con A-mediated mitogenesis was, however, poor in summer and abrogated in winter. Removal of Sephadex G 10-adherent cells from the SC suspensions did not inhibit the lymphoproliferative responsiveness to Con A in spring. This supports the view that the weak responses in summer and winter are essentially due to lack of competent T lymphocytes.
D000818:Animals; D002458:Cell Fractionation; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D008623:Mercaptoethanol / Q000494:pharmacology; D010586:Phagocytes / Q000276:immunology; D012621:Seasons; D012911:Snakes / Q000276:immunology*
D003208:Concanavalin A; D008623:Mercaptoethanol