[Effect of the cytoplasm of irradiated cells on chromatin degradation in mouse thymocytes and hepatocytes].
The addition of a cytoplasmic fraction, isolated from cells 3h after irradiation of mice, to exposed or intact thymocyte nuclei causes a 2- or 3-fold acceleration of chromatin degradation in the nuclei incubated in conditions optimum for activity of Ca2+,Mg2+-dependent endonuclease to be manifest. In contrast to thymocytes, no chromatin degradation products are found in liver cells of irradiated mice. The cytoplasmic fraction isolated from hepatocytes of irradiated animals fails to activate chromatin degradation in thymocyte nuclei.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D002843:Chromatin / Q000378:metabolism / Q000528:radiation effects*; D003593:Cytoplasm / Q000528:radiation effects*; D005720:Gamma Rays; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013950:Thymus Gland / Q000378:metabolism / Q000528:radiation effects*
D002843:Chromatin
Vliianie tsitoplazmy obluchennykh kletok na degradatsiiu khromatina v timotsitakh i gepatotsitakh mysheĭ.