[Proteinization and biotransformation of pantothenic acid in the liver during the activation of lipogenesis].
White female rats received a balanced synthetic ration (control) or a ration devoid of pantothenic acid (PAA) during 3 weeks. After 36-hour fasting adaptive hyperlipogenesis was induced by feeding the animals with a high-carbohydrate ration, then [114-C]-PAA (sodium salt, 182 nmol/kg) was administered with intervals of 3, 6, 24 hours up to 1 hour before decapitation. Radioactivity of the rats' boiled liver extracts depended on the hyperlipogenesis stage, its level rose progressively, in the control and reached the maximum in PAA-deficient animals by hour 6 after the feeding resumption. The PAA-deficient animals possessed a high PAA-accumulating capacity of the liver and cytosole of the liver including non-covalent radionuclide binding by protein complexes. CoA-synthesizing capacity of the liver in the control animals, evaluated by the biotransformation of the labeled vitamin with CoA precursors of CoA, was intensified with the lipogenesis activation; in vitamin-deficiency CoA biosynthesis was accelerated more than two-fold as compared to the control at the initial and extended periods of hyperlipogenesis (3.6 h). The differences in proteinization and biotransformation of PAA in the liver of control and PAA-deficient animals disappeared by 25 h of adaptive hyperlipogenesis.
D000818:Animals; D001711:Biotransformation; D005260:Female; D008053:Lipid Mobilization; D008099:Liver / Q000378:metabolism*; D010205:Pantothenic Acid / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats
D010205:Pantothenic Acid
Proteidizatsiia i biotransformatsiia pantotenovoĭ kisloty v pecheni pri aktivatsii lipogeneza.