Determination of amine oxidases in tissues by peroxidation-induced chemiluminescence of phthalazines.
A method is proposed for the determination of the activity of amine oxidases in purified samples and tissue homogenates. The method is based on the chemiluminescence of luminol and other cyclic hydrazides elicited by the horseradish peroxidase-catalyzed peroxidation using H2O2 produced in the amine oxidase reaction. Several aspects of the chemiluminescence method for determining enzymatic activity in crude tissue extracts are discussed.
D000818:Animals; D001932:Brain Neoplasms / Q000201:enzymology; D002417:Cattle; D006735:Horseradish Peroxidase; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008163:Luminescent Measurements; D008165:Luminol; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis* / Q000097:blood; D010793:Phthalazines; D011724:Pyridazines; D013552:Swine
D010793:Phthalazines; D011724:Pyridazines; D008165:Luminol; D006735:Horseradish Peroxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors