Kinetic study of rheumatoid factor influence on complement-mediated modulation of immune precipitation.
Human monoclonal IgM kappa rheumatoid factor PA (mRF) expressed various effects on complement-dependent modulation of ovalbumin/antiovalbumin lattice formation measured kinetically by laser light scattering (LLS): (1) in the absence of complement, mRF (44.2-177 U/ml) caused enhancement of LLS in a dose-dependent manner; (2) mRF partially prevented expression of complement-mediated inhibition of lattice formation and, at the same time, complement inhibits mRF-dependent LLS enhancement; (3) complement caused disruption of lattice-mRF bonds during solubilization of preformed lattice-mRF complex as demonstrated by specific mRF activity in supernatants. This effect is dependent on complement activity and on both complement and mRF concentrations; (4) ovalbumin/antiovalbumin complexes gradually lost the ability to react with mRF during the first 2 min of complement-mediated inhibition of lattice formation.
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D011232:Chemical Precipitation; D003165:Complement System Proteins / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007700:Kinetics; D010047:Ovalbumin / Q000276:immunology; D012217:Rheumatoid Factor / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007075:Immunoglobulin M; D010047:Ovalbumin; D003165:Complement System Proteins; D012217:Rheumatoid Factor