Effect of chromate ion on the membrane of established human cells as measured by uptake of a permeant lipophilic cation.
Cells from the established human cell line NHIK3025 concentrate the permeant lipophilic cation triphenylmethylphosphonium (TPMP+) against a concentration gradient, indicating the existence of an electrical potential across the cell membrane (interior negative). Cells exposed to potassium chromate or dichromate (7.7 mumol Cr/l) for 2 h subsequently showed reduced uptake of TPMP+, whereas similar exposure to a trivalent chromium salt (7.7 mumol/l chromic chloride) had similar uptake of TPMP+ as control cells. These preliminary results suggest that a primary effect of chromate on these cells is to reduce the electrical potential across the cell membrane.
D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002840:Chromates / Q000494:pharmacology*; D006801:Humans; D009861:Onium Compounds / Q000378:metabolism*; D014320:Triphenylmethyl Compounds / Q000378:metabolism*
D002840:Chromates; D009861:Onium Compounds; D014320:Triphenylmethyl Compounds; C011561:triphenylmethylphosphonium