Detection of IgE antibody-forming cells by passive cutaneous anaphylaxis using cell extract from lymphoid organs.
IgE and IgG2a antibody-forming cells in the lymphoid organs of rats were detected by passive cutaneous anaphylaxis (PCA) reaction using the extract from the tissue. The amount of antigen-specific IgE and IgG2a antibodies in the tissue extract could be expressed as PCA titers in which extract from 10(8) cells/ml was used as starting dilution. The total amount of antigen-specific IgE antibody produced in mouse IgE-producing hybridoma cells was calculated by maximum dilution of the extract to the minimal amount of IgE antibody inducing PCA reaction, i.e., 4 ng/ml. In the case of the extract from mouse IgE antibody-forming hybridomas, the minimal number of hybridoma cells to be detected by this method proved to be 2-3 X 10(4) cells/ml.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D006825:Hybridomas / Q000032:analysis; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology*; D014020:Tissue Extracts / Q000276:immunology
D007074:Immunoglobulin G; D014020:Tissue Extracts; D007073:Immunoglobulin E