title: Studies on the effect of inflammation on the synthesis of glucosylated intermediates of glycoprotein biosynthesis in rat liver microsomes.
abstract: Glycoprotein biosynthesis is substantially increased in liver during experimental inflammation. This study describes the effect of inflammation on the incorporation of labelled Glc from UDP-Glc into glucosylated lipid-linked intermediates of glycoprotein biosynthesis in liver microsomes. Maximum incorporation of Glc into lipid sugar and lipid oligosaccharide fractions occurred using microsome fractions from rats suffering from inflammation for 48-72 hr. Incorporation of Glc into lipid-sugar fractions was increased about three-fold over controls and incorporation into lipid-oligosaccharide fractions was increased about four-fold compared to controls. Maximum incorporation of Glc into lipid-sugar and lipid-oligosaccharide occurred at pH 6.0 and pH 5.2, respectively.
mesh_terms: D000818:Animals; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007249:Inflammation / Q000378:metabolism*; D050356:Lipid Metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D051381:Rats
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides