title: [RAMT cell line as a substrate for the propagation of vaccinal viral strains].
abstract: Properties of the PAMT cell line which are deficient in hypoxanthine guanyl phosphoribosiltransferase were studied. The PAMT cell line derived from African green monkey kidney tissue may be identified by selective nutrient media, contains no contaminants, is not tumorigenic, is susceptible to a wide range of viruses. Titres of poliomyelitis, tick-borne encephalitis, and carnivore plague viruses were similar in cell cultures grown in the medium with 10% serum and in those adapted to growth in the medium containing 1% serum. The properties of PAMT cell line allow it to be used in manufacture of killed virus vaccines.
mesh_terms: D000818:Animals; D001375:Azaguanine / Q000037:antagonists & inhibitors; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology; D004351:Drug Resistance; D006801:Humans; D015122:Mercaptopurine / Q000037:antagonists & inhibitors; D013866:Thioguanine / Q000037:antagonists & inhibitors; D013997:Time Factors; D014765:Viral Vaccines; D014776:Virus Cultivation / Q000379:methods*; D014780:Viruses / Q000254:growth & development
chemical_list: D003470:Culture Media; D014765:Viral Vaccines; D015122:Mercaptopurine; D013866:Thioguanine; D001375:Azaguanine
vernacular_title: Liniia kletok RAMT kak substrat dlia razmnozheniia vaktsinnykh shtammov virusov.