Regulation of free cytosolic Ca2+ in the isolated guinea pig gastric chief cells.
Stimulation with COOH-terminal octapeptide of cholecystokinin (CCK8) or carbachol resulted in a rapid increase in Quin-2 fluorescence of isolated guinea pig gastric chief cells, whereas histamine, vasoactive intestinal peptide, secretin or forskolin had no effect. The minimum effective dose of CCK8 or carbachol to elicit the rise in Quin-2 fluorescence was almost similar to that for pepsinogen secretion. Removal of Ca2+ from extracellular medium or Ca2+ channel blockers did not affect CCK8- or carbachol-induced increase in Quin-2 fluorescence. Moreover, following addition of CCK8, carbachol was unable to stimulate a second increase in Quin-2 fluorescence. These results suggest that CCK8 and carbachol share common Ca2+ pools and an increase in free cytosolic Ca2+ concentration may mediate CCK8- or carbachol-induced pepsinogen secretion from gastric chief cells.
D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D005456:Fluorescent Dyes; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D010435:Pepsinogens / Q000378:metabolism; D012844:Sincalide / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D000634:Aminoquinolines; D005456:Fluorescent Dyes; D010435:Pepsinogens; D002217:Carbachol; D012844:Sincalide; C035739:Quin2; D002118:Calcium