Glycoprotein profiles of macrophages at different stages of activation as revealed by lectin binding after electrophoretic separation.
Glycoprotein profiles of rat macrophages (M phi) at different stages of activation were studied by examining the reactivity of various lectins to the glycoproteins separated by polyacrylamide gel electrophoresis. Ricinus communis agglutinin 1 (RCA1) revealed several components including glycoproteins of Mr 160 kDa and 65 kDa prominent in resident M phi. A pokeweed mitogen (PWM) isolectin, Pa-4, recognizes branched poly(N-acetyllactosamine)-type carbohydrate chains, and revealed a significant increase in glycoproteins of Mr ranging from 70 kDa to 150 kDa on thioglycolate-elicited M phi. Increased reactivity of PWM to thioglycolate-elicited M phi was observed by direct binding of 125I-labeled Pa-4 to intact or glutaraldehyde-fixed M phi. Histochemical staining of formaldehyde-fixed M phi in vitro with biotinylated Pa-4 was consistent with the gel analysis, that is, resident M phi had no reactivity while thioglycolate-elicited M phi showed slight reactivity. Alveolar and intratumoral M phi bound more Pa-4 than resident or thioglycolate-elicited M phi. The PWM isolectin may therefore serve as a marker for an early stage of M phi activation.
D000818:Animals; D002241:Carbohydrates / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000032:analysis* / Q000145:classification / Q000166:cytology; D008325:Mammary Neoplasms, Experimental / Q000473:pathology; D008565:Membrane Proteins / Q000032:analysis*; D010529:Peritoneal Cavity / Q000166:cytology; D011043:Pokeweed Mitogens / Q000378:metabolism; D011485:Protein Binding; D011650:Pulmonary Alveoli / Q000166:cytology; D051381:Rats; D011916:Rats, Inbred F344; D013864:Thioglycolates / Q000494:pharmacology
D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011043:Pokeweed Mitogens; D013864:Thioglycolates