A simple technique for increased sensitivity in immunoelectrophoresis by the use of antiserum against IgG.
Precipitates in two-dimensional immunoelectrophoresis (2D-IE) plates can be amplified by the application of an antiserum against IgG after the second dimension run. Thereby, the sensitivity of the IE method is increased; faint precipitates and antigen-antibody reactions close to but below the level of forming recognizable precipitates are made visible. The advantage of the method was demonstrated for a multilinear Mycobacterium phlei system.
D000818:Animals; D011232:Chemical Precipitation; D007106:Immune Sera / Q000276:immunology*; D007122:Immunoelectrophoresis / Q000379:methods*; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D011817:Rabbits
D007106:Immune Sera; D007074:Immunoglobulin G