A simplified method for the isolation of highly purified bovine retinal S antigen.
Retinal S antigen is a potent autoantigen used for the induction of experimental allergic uveoretinitis (EAU). EAU is an organ-specific disease and shows many similarities to other autoimmune diseases such as experimental encephalomyelitis. This paper describes the preparation of highly purified S antigen by using a one-step ion-exchange method. High yields of the protein were obtained. S antigen prepared by this method induces a prolonged posterior uveoretinitis with cellular infiltration in the vitreous and specific loss of retinal photoreceptor cells. The purity of the protein was checked by silver-stained SDS-polyacrylamide gels and immunoblotting techniques.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D019393:Arrestin; D002417:Cattle; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000302:isolation & purification*; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits / Q000276:immunology
D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D007106:Immune Sera