Purification of foetal steroid-binding protein from human serum by affinity chromatography on 5 alpha-androstane-3 beta,17 beta-diol 3-hemisuccinate-aminohexyl-Sepharose 6B.
In order to develop an immunoassay for foetal steroid-binding protein in human serum, which is impossible to assay quantitatively in normal samples by conventional ligand-binding techniques, the protein was purified by salt precipitation, affinity chromatography and gel filtration. Elution was by competing ligand or alkaline pH. The purified protein was further characterized and a highly specific antiserum was raised in rabbits.
D015113:Androstane-3,17-diol / Q000031:analogs & derivatives; D002352:Carrier Proteins / Q000097:blood / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005326:Fetal Proteins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D008970:Molecular Weight; D011247:Pregnancy; D012738:Sex Hormone-Binding Globulin / Q000302:isolation & purification
D002352:Carrier Proteins; D005326:Fetal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D012738:Sex Hormone-Binding Globulin; C051508:androstane-3,17-diol-3-hemisuccinate-aminohexylsepharose 6B; C051517:fetal steroid-binding protein, human; D015113:Androstane-3,17-diol