In vitro and in vivo growth of an intraocular retinoblastoma-like tumour in F-344 rats.
Retinoblastoma-like cells grew in colonies on the bottom of tissue culture flasks. The population doubling time was 19 h. Tumour cells from cell cultures had a 39% plating efficiency, and fresh tumour cells from intraocular tumours had a 32% plating efficiency in colony forming assays. Inoculation of 1.5 X 10(4) tumour cells in the vitreous of F-344 rats resulted in a 100% tumour take and regularly growing tumours with a doubling time of 3 days. The tumour take-rate was not changed in wholebody immunosuppressed animals. The tumour volume was assessed under a stereo microscope, and it was possible to divide the tumours into 4 groups according to the number of intraocular tumour cells. Tumour growth caused eye perforation in 89% of the inoculated eyes. Spontaneous tumour regression was not seen in non-perforation groups. Immunosuppression with whole-body irradiation and dense traumatic cataract had no significant effect on the growth. It is concluded that this animal retinoblastoma-like tumour is suitable for quantitative therapy studies in vivo and in vitro.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D005134:Eye Neoplasms / Q000276:immunology / Q000473:pathology* / Q000628:therapy; D007165:Immunosuppression Therapy; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D012175:Retinoblastoma / Q000276:immunology / Q000473:pathology* / Q000628:therapy