Solvatochromic solvent polarity measurements and selectivity in reversed-phase liquid chromatography.
The ET(30) polarity values of binary acetonitrile-water and methanol-water mobile phases, used in reversed-phase liquid chromatography, were measured and compared with methylene selectivity (alpha CH2) for both traditional siliceous bonded phases and for a polystyrene-divinylbenzene resin reversed-phase material. The variation in methylene selectivity for both was found to correlate best with percent organic solvent in methanol-water mixtures, while the ET(30) polarity provided the best correlation in acetonitrile-water mixtures. The polymeric resin column was found to provide higher methylene selectivity than the siliceous bonded phase at all concentrations of organic solvent.
D000097:Acetonitriles; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002853:Chromatography, Liquid / Q000295:instrumentation*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D012997:Solvents