K23. A low-incidence antigen in the Kell blood group system identified by biochemical characterization.
An antibody in the serum of a gravida 4, para 3 woman reacted with red cells from two of her children, her husband, and his mother, but with none of more than 2100 reference red cell samples and blood samples from donors. The reactive antigen was inactivated by 2-aminoethylisothiouronium bromide or dithiothreitol-papain treatment. The antigen was immunoprecipitated from paternal red cells with maternal antibody and shown to migrate by sodium dodecylsulfate polyacrylamide gel electropheresis as a single protein of approximately 93,000 daltons. After transfer to nitrocellulose paper by Western blotting, the protein reacted with a rabbit antibody specific for Kell protein. The chemical inactivation and electrophoretic findings were characteristic of Kell group antigens. The reaction with the rabbit antibody establishes that the "new" low incidence antigen was an epitope on Kell group protein and must be coded for by the Kell gene. It has been designated K23.
D001789:Blood Group Antigens / Q000276:immunology*; D003298:Coombs Test; D004910:Erythrocyte Membrane / Q000276:immunology*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007518:Isoantibodies / Q000276:immunology; D007626:Kell Blood-Group System / Q000235:genetics / Q000276:immunology*; D008431:Maternal-Fetal Exchange; D008565:Membrane Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology; D010375:Pedigree; D011247:Pregnancy
D001789:Blood Group Antigens; D007518:Isoantibodies; D007626:Kell Blood-Group System; D008565:Membrane Proteins