Regulatory mechanisms of UDP-glucuronic acid biosynthesis in cultured human skin fibroblasts.
Kinetic parameters and regulatory properties of UDPGDH extracted from cultured human skin fibroblasts were determined and compared with those of UDPGDH from cornea and epiphysial-plate cartilage. Fibroblast enzyme showed an affinity for UDPG 7 times higher than cartilage enzyme and 42 times higher than cornea enzyme. UDP-xylose acted as a co-operative allosteric inhibitor, but under the same experimental conditions fibroblast enzyme was significantly less inhibited. These results were in agreement with the different GAG production of the cells we studied. Fibroblast UDPGDH activity was regulated by the NAD/NADH ratio and it was also affected by modifications of extracellular matrix composition. A significant increase of UDPGDH affinity for UDPG was observed after the treatment of the monolayers with Chase ABC.
D002478:Cells, Cultured; D002808:Chondroitin Lyases / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D014535:Uridine Diphosphate Glucuronic Acid / Q000096:biosynthesis* / Q000502:physiology; D014539:Uridine Diphosphate Sugars / Q000096:biosynthesis*; D014540:Uridine Diphosphate Xylose / Q000494:pharmacology; D014574:Uronic Acids / Q000096:biosynthesis
D006025:Glycosaminoglycans; D014539:Uridine Diphosphate Sugars; D014574:Uronic Acids; D009243:NAD; D014535:Uridine Diphosphate Glucuronic Acid; D014540:Uridine Diphosphate Xylose; D002808:Chondroitin Lyases