[Physicochemical characteristics of sorbitol dehydrogenase from the cytoplasm of the liver cells of susliks].
Some physico-chemical properties of sorbitol dehydrogenase from squirrel liver cell cytoplasm have been investigated. Non-linear dependence of enzyme activity upon media pH is shown. Activity manifests only in the presence of NAD that can't be replaced by NADP. The enzyme exhibits stereospecificity: it dehydrates polyol of any length, the second and the fourth carbon atoms have common L-configuration concerning the first carbon atom. A diffusion zone with Rf = 0,09 is revealed on the electrophoregram. Three thin zones of activity about pH 7,2 are exposed by isoelectrofocusing method.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003593:Cytoplasm / Q000032:analysis / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007064:L-Iditol 2-Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000032:analysis / Q000201:enzymology*; D012589:Sciuridae / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*
D013401:Sugar Alcohol Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase
Fiziko-khimicheskie kharakteristiki sorbitoldegidrogenazy iz tsitoplazmy kletok pecheni suslikov.