Microsomal phospholipase A2 is activated by surfactant toxins in 3T3 mouse fibroblasts.
In 3T3 mouse fibroblasts that were treated by surfactant toxins such as staphylococcal delta toxin, melittin from bee venom and lysolecithin, microsomal phospholipase A2 (PLA2) was activated. However, these toxins did not activate PLA2 when added to cell-free homogenates or microsomal preparations. The maximal activation was achieved at 1 mM Ca2+ pH 8.5. The microsomal PLA2 stimulated by these toxins had a high fatty acid specificity (C-2 position) toward arachidonic acid.
D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D001514:Bee Venoms / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002458:Cell Fractionation; D002460:Cell Line; D004789:Enzyme Activation; D005347:Fibroblasts / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008555:Melitten / Q000494:pharmacology*; D051379:Mice; D008861:Microsomes / Q000187:drug effects* / Q000201:enzymology; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013379:Substrate Specificity
D001427:Bacterial Toxins; D001514:Bee Venoms; D008244:Lysophosphatidylcholines; C025971:staphylococcal delta toxin; D008555:Melitten; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium