title: Stratum corneum lipid liposomes: calcium-induced transformation into lamellar sheets.
abstract: The epidermal water barrier in mammalian stratum corneum is formed of broad lamellar sheets of lipids consisting principally of ceramides (40%), cholesterol (25%), cholesteryl sulfate (10%), and free fatty acids (25%). Such lipid mixtures have been shown to form lipid bilayers in the form of small, unilamellar liposomes when sonicated at 80 degrees C in water containing Tris buffer and 100 mM NaCl. In the present study it is shown that such liposomes are slowly transformed into large unilamellar liposomes and then into broad lamellar sheets after the addition of stoichiometric amounts of calcium chloride. The presence of free fatty acids was a necessary condition for this calcium-induced fusion. These observations may provide a useful analogy for the transformation of flattened liposomes into broad lamellar sheets that occurs during transition of epidermal granular cells into corneocytes.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002518:Ceramides / Q000032:analysis / Q000302:isolation & purification; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D004817:Epidermis / Q000032:analysis* / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis*; D008081:Liposomes; D008561:Membrane Fusion / Q000187:drug effects; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis; D013552:Swine
chemical_list: D002518:Ceramides; D002788:Cholesterol Esters; D008055:Lipids; D008081:Liposomes; D010169:Palmitic Acids; D019308:Palmitic Acid; D002784:Cholesterol; C007045:cholesteryl sulfate; D002118:Calcium