A micromethod for the estimation of blood dolichol.
A micromethod for the estimation of dolichol in blood was developed using high performance liquid chromatography. This method can be applied to whole blood or plasma. With detector sensitivity set at 0.005 Absorbance Unit Full Scale, samples as little as 50 microliter are sufficient to carry out the determination. Blood samples from the tail vein of inbred strains of C57 BL/6 NNia-1 mice 3, 6, 12 and 24 mo of age were examined. Blood dolichol levels decreased with age from 163.3 ng/ml at 6 mo to 110.1 ng/ml at 24 mo. The major dolichol homologs were C-85 (10.4%), C-90 (41.6%), C-95 (38.0%) and C-100 (8.1%).
D000367:Age Factors; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000097:blood*; D004286:Dolichols / Q000097:blood*; D051379:Mice; D008810:Mice, Inbred C57BL; D008832:Microchemistry / Q000379:methods
D004224:Diterpenes; D004286:Dolichols