Introduction of deoxyribonucleoside triphosphates into intact cells by electroporation.
A simple method, employing high-voltage electric discharge (electroporation), was developed to introduce phosphorylated nucleosides into the cytoplasm of viable cells. HL-60 leukemia cells permeabilized by this technique remained viable and incorporated deoxyribonucleoside triphosphates into nuclear DNA. Furthermore, DNA synthesis was depressed for at least 24 h in HL-60 cells made permeable to 1-beta-D-arabinosylcytosine 5'-triphosphate by this methodology. Electroporation was found to be applicable to the permeabilization of a wide variety of cell lines in culture to nucleotides, suggesting that this methodology may be useful for the introduction into intact cells of a wide variety of molecules that are not normally transported effectively.
D002460:Cell Line; D002463:Cell Membrane Permeability; D004247:DNA / Q000096:biosynthesis; D003854:Deoxyribonucleotides / Q000008:administration & dosage*; D004560:Electricity; D006801:Humans
D003854:Deoxyribonucleotides; D004247:DNA