Quantitation of apolipoprotein C-III in normal and in hyperlipaemic serum samples by enzyme-linked immunosorbent assay.
A specific and sensitive Sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of apolipoprotein C-III, a major apolipoprotein of human very low density lipoproteins is described. The assay is non-competitive and it uses the same affinity isolated sheep antibody both for coating the wells and as conjugate with alkaline phosphatase. Total serum apo C-III was determined in a normal population of 24 men and 21 women. The difference was not statistically significant. In both sexes apo C-III concentration correlated positively with the serum triglyceride levels. In patients with hyperlipoproteinemia, apo C-III levels were increased.
D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D006951:Hyperlipoproteinemias / Q000097:blood*; D008297:Male; D014280:Triglycerides / Q000097:blood
D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D014280:Triglycerides; D002784:Cholesterol