title: Primary structure of the hypertrehalosaemic factor II from the corpus cardiacum of the Indian stick insect, Carausius morosus, determined by fast atom bombardment mass spectrometry.
abstract: The primary structure of hypertrehalosaemic factor II, isolated from the corpus cardiacum of the Indian Stick Insect Carausius morosus, has been assigned as Glu-Leu-Thr-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH2 from its fast atom bombardment (FAB) mass spectrum and metastable scans of its FAB spectrum. The structure assigned shows close homology to other insect neuropeptides. A synthetic sample of this peptide gave the same FAB spectra, reversed-phase high-performance liquid chromatographic behavior, and biological behavior as the natural material. Mass spectrometric fragmentation of the synthetic peptide was examined by B/E linked scan and MIKES techniques in a two-sector mass spectrometer and by the MS/MS technique in a four-sector (tandem) spectrometer.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007301:Insect Hormones / Q000138:chemical synthesis / Q000302:isolation & purification; D007313:Insecta; D013058:Mass Spectrometry; D009842:Oligopeptides
chemical_list: D007301:Insect Hormones; D009842:Oligopeptides; C045412:hypertrehalosaemic factor (Carausius morosus)