Comparative phenotypic and ultrastructural characteristics of OKT6-positive cells in normal peripheral blood (adult and infant), in cord blood and in epidermis.
Simple and rapid procedures have been developed to isolate T6-positive cells in epidermal cell suspensions, in peripheral blood of adult, and in cord blood. The isolated T6-positive cells were tested by double immunogold labelling at ultrastructural level to analyze their phenotype (T6/HLA-DR antigens) and their morphological features in order to understand their origin, their fate, and their relationship to the early precursors of dendritic cells, immature neonatal lymphoid subsets, and immature macrophagic leukocytes.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology*; D006046:Gold; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000145:classification / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D006046:Gold