Isolation of metastatic B16 melanoma variants using anti-Met 72 monoclonal antibodies and flow cytometry.
Analysis of a number of B16 melanoma clones has revealed a high correlation between metastatic activity and the quantitative expression of a 72,000 dalton glycoprotein, Met 72. In the present study, metastatic tumor cell variants have been directly isolated from a heterogeneous, poorly metastatic melanoma (B16-F1) by anti-Met 72 monclonal antibodies and cell sorting procedures. These studies provide direct proof that Met-72 antigens are in fact surface markers of B16 melanoma metastatic variants and may provide the means of monitoring their presence, influence and autonomy during tumor progression.
D000818:Animals; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis; D002469:Cell Separation; D005434:Flow Cytometry; D008175:Lung Neoplasms / Q000556:secondary; D008546:Melanoma, Experimental / Q000032:analysis / Q000276:immunology / Q000473:pathology*; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D008565:Membrane Proteins; D009363:Neoplasm Proteins