In vitro synthesis of apo-A-IV and apo-C by liver and intestinal mRNAs from lean and obese Zucker rats.
The relative content and expression of mRNA coding for apolipoproteins A-IV and C in liver and intestinal cells have been studied in obese hyperlipemic (fa/fa) and lean (Fa/-) Zucker rats. The RNA were translated in a rabbit reticulocyte lysate system using 35S methionine as label. Apo-A IV and apo-C were immunoprecipitated with specific antibodies and characterized by electrophoresis and autoradiography. The content and expression of mRNA specific for apo-A-IV and apo-C was much higher and the liver of obese than of lean rats. The apo-A-IV and apo-C synthetic capacity of intestinal cells from fa/fa and Fa cells from fa/fa and Fa rats was similar suggesting that the lipoprotein overproduction, already described in obese Zucker rats, is mainly from hepatic origin.
D000818:Animals; D001054:Apolipoproteins A / Q000096:biosynthesis* / Q000235:genetics; D001056:Apolipoproteins C / Q000096:biosynthesis* / Q000235:genetics; D002784:Cholesterol / Q000378:metabolism; D005786:Gene Expression Regulation; D005838:Genotype; D007413:Intestinal Mucosa / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains / Q000502:physiology*; D011924:Rats, Zucker / Q000502:physiology*; D014158:Transcription, Genetic; D014280:Triglycerides / Q000378:metabolism
D001054:Apolipoproteins A; D001056:Apolipoproteins C; D010743:Phospholipids; D012333:RNA, Messenger; D014280:Triglycerides; C022205:apolipoprotein A-IV; D002784:Cholesterol