A signal regulating mouse histone H4 mRNA levels in a mammalian cell cycle mutant and sequences controlling RNA 3' processing are both contained within the same 80-bp fragment.
Fragments from the 3' end of a mouse histone H4 gene, when introduced into transcription units controlled by the SV40 early promoter, yield correctly processed RNA with histone-specific 3' ends, both in monkey and mouse cell lines. The processed RNA is regulated in parallel with endogenous H4 mRNAs in 21-Tb cells, a temperature-sensitive mouse mastocytoma cell cycle mutant that is specifically blocked in G1 phase at the non-permissive temperature. Mutational analyses of the H4 gene fragment indicate that the minimal sequences for this regulation and for RNA 3' processing are both contained within the same 80 bp. This fragment contains two histone-specific, highly conserved sequence elements that are located at the 3' end of histone mRNA and in the adjacent spacer region, respectively. Our data suggest that the observed cell cycle regulation is achieved either at RNA 3' processing or at some later step involving the conserved 3'-terminal sequence element of mature histone mRNA.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002522:Chlorocebus aethiops; D002872:Chromosome Deletion; D006657:Histones / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D021382:Protein Sorting Signals / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
D006657:Histones; D021382:Protein Sorting Signals; D012333:RNA, Messenger