A comparison of the radiation-induced mutation at the HGPRT locus in V79 and BHK cells.
Mutation response at the HGPRT locus has been compared in two differing cell lines: V79/4, an aneuploid Chinese-hamster fibroblast line with a complement of 20 chromosomes, and BHK21-C13, a diploid Syrian-hamster fibroblast line with a complement of 44 chromosomes. The data presented show that BHK is slightly more radiosensitive than V79/4; however, the toxicity curves and expression times are similar for both cell lines. If radiosensitivity is taken into account, a common line can be drawn for radiation mutagenesis. We conclude from the data that radiation-induced mutagenesis is broadly equivalent in the two cell lines examined, and is not dependent on the chromosome complement.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D005796:Genes / Q000528:radiation effects; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007668:Kidney; D008168:Lung; D008297:Male; D008647:Mesocricetus / Q000235:genetics; D011836:Radiation Tolerance; D013866:Thioguanine / Q000494:pharmacology
D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine