A case of a newborn infant with Hb M Iwate.
Hemoglobin of a newborn infant who was suspected to hereditarily have Hb M Iwate was examined. The infant hemolysate was separated into five fractions by column chromatography on Amberlite CG-50, and two of these fractions showed absorption spectra corresponding with that of Hb M Iwate. Five bands were found after the isoelectric focusing of the hemolysate, and two of these bands were brown. The two Hb M fractions obtained by column chromatography was focused to the positions of the brown bands. One of these Hbs M corresponded with Hb M Iwate (alpha M2 beta 2) from an adult carrier of this trait, but the other was not found in adult hemolysates. The latter species of Hb M was shown to be composed of the abnormal alpha chain and the normal gamma chain (alpha M2 gamma 2) by chain analysis, and was assumed to be specific for infants. A quantitative estimation of the hemoglobins in the infant hemolysate showed that there was no difference between the relative quantities of the fetal and adult forms of Hb M Iwate.
D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D006449:Hemoglobin M / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D007525:Isoelectric Focusing; D008297:Male; D013053:Spectrophotometry
D006455:Hemoglobins, Abnormal; C027542:hemoglobin M Iwate; D006449:Hemoglobin M