title: Adaptation of coenzyme stimulation assays for the nutritional assessment of vitamins B1, B2 and B6 using the Cobas Bio centrifugal analyser.
abstract: Adaptation of coenzyme stimulation assays for the nutritional assessment of thiamine, riboflavin and pyridoxine on the Cobas Bio centrifugal analyser are described. Whole blood was collected into acid-citrate dextrose, which preserves the erythrocytes, prior to assay for several days. Washed erythrocytes stored at -70 degrees C and subsequently thawed, showed altered enzyme activities. The methods offer improved precision over existing procedures and take advantage of the high throughput capabilities of the instrumentation.
mesh_terms: D000856:Anorexia Nervosa / Q000150:complications / Q000201:enzymology; D002498:Centrifugation; D004796:Clinical Enzyme Tests; D003067:Coenzymes / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D008433:Mathematics; D009243:NAD / Q000378:metabolism; D011736:Pyridoxine / Q000097:blood*; D012256:Riboflavin / Q000097:blood*; D013831:Thiamine / Q000097:blood*; D014174:Transketolase / Q000097:blood; D014804:Vitamin B Deficiency / Q000097:blood / Q000175:diagnosis*
chemical_list: D003067:Coenzymes; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D005980:Glutathione Reductase; D014174:Transketolase; D005978:Glutathione; D011736:Pyridoxine; D012256:Riboflavin; D013831:Thiamine