Identification of environmental Vibrio vulnificus isolates with a DNA probe for the cytotoxin-hemolysin gene.
We screened 44 lactose-positive Vibrio strains isolated from the marine environment for homology with a 3.2-kilobase DNA fragment encoding the Vibrio vulnificus cytotoxin-hemolysin gene. All 29 marine isolates identified as V. vulnificus on the basis of numerical taxonomy and DNA-DNA hybridization studies hybridized with the cytotoxin gene probe, as did all V. vulnificus reference strains. Homologous gene sequences were identified in no other lactose-positive marine vibrio isolates nor in 10 other Vibrio species.
D000818:Animals; D003603:Cytotoxins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010125:Ostreidae / Q000382:microbiology; D012623:Seawater; D012689:Sequence Homology, Nucleic Acid; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014871:Water Microbiology
D003603:Cytotoxins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins