Age related changes in fluidity of rat renal brushborder membrane vesicles.
Fluorescence anisotrophy of 1,6 diphenyl-1,3,5-hexatriene was determined in renal brushborder membranes prepared from rats 7, 14, 21 and 28 days of age, and adults, from 5 degrees C to 45 degrees C. There is a parallel relationship between temperature and mean fluorescence anisotrophy in the different age groups with a progressive decrease in fluidity with age. There is no phase transition apparent in membranes from any age group as evidenced by the lack of a fluorescence polarization "break point". There is also a linear relationship between limiting hindered anisotrophy and previously determined values for the height of the Na+-proline overshoot. This suggests that the physical characteristics of the renal brushborder membrane responsible for differences in fluidity are related to age-dependent transport alterations.
D000375:Aging / Q000502:physiology*; D000818:Animals; D004161:Diphenylhexatriene; D005454:Fluorescence Polarization; D007668:Kidney / Q000648:ultrastructure*; D008297:Male; D008560:Membrane Fluidity; D008871:Microvilli / Q000502:physiology*; D051381:Rats
D004161:Diphenylhexatriene