title: Treatment of rats with hCG induces inflammation-like changes in the testicular microcirculation.
abstract: Adult rats were injected subcutaneously with 50 i.u. hCG and vascular permeability was compared to that in saline-treated control rats by two independent methods. At 4 h after hCG treatment the rats were injected intra-arterially (i.a.) with FITC-labelled macromolecular dextran (Mr 150,000) and the testicular microcirculation was studied in vivo by using a fluorescence microscope. Other rats were injected i.a. with a suspension of colloidal carbon and the location of leaking blood vessels was recorded in sections from the testes by light and electron microscopy. In hCG-treated animals leucocytes were found adhering to the endothelium in post-capillary venules and in these venular segments dextran was leaking into the interstitium. Carbon particles were deposited in the walls of post-capillary venules and leucocytes migrated through open interendothelial cell gaps in hCG-treated animals. In control animals leucocyte adhesion and migration were not observed, the injected dextran remained in the circulation and the blood vessels were not labelled by carbon. It is suggested that the hCG-induced increase in testicular interstitial fluid volume, like the tissue oedema in inflammation, is caused by a leucocyte-mediated increase in venular permeability.
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D007249:Inflammation; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000098:blood supply* / Q000187:drug effects / Q000648:ultrastructure; D014655:Vascular Resistance / Q000187:drug effects
chemical_list: D006063:Chorionic Gonadotropin