Comparison of five techniques for the determination of protein content in mixed human saliva.
This study was conducted to assess the relative accuracy of five different assay techniques for the determination of protein concentration in human mixed saliva. The protein concentration of paraffin-stimulated saliva from 20 individuals was determined using the biuret reaction, the Lowry assay, a modified Lowry technique using bicinchoninic acid, and two dye-binding assays. Using bovine serum albumin as the standard, mean values ranged from 0.67 to 2.37 mg/ml. The use of bovine serum albumin, trypsinogen, lysozyme, bovine pancreatic ribonuclease, and poly-L-lysine as standards with the five different assay techniques to measure protein concentration of pooled mixed saliva from the above subjects produced results ranging from 0.74 to 65.5 mg/ml. The protein concentration obtained for this saliva sample by amino acid analysis was consistent with the value obtained for the biuret reaction using any of the five different standard proteins. Thus, the protein concentration obtained for human saliva depends upon both the technique used and the protein standard.
D000596:Amino Acids / Q000032:analysis; D004396:Coloring Agents; D006801:Humans; D006851:Hydrochloric Acid; D010756:Phosphoric Acids; D011485:Protein Binding; D011804:Quinolines; D012463:Saliva / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D013053:Spectrophotometry
D000596:Amino Acids; D004396:Coloring Agents; D010756:Phosphoric Acids; D011804:Quinolines; D012471:Salivary Proteins and Peptides; C047117:bicinchoninic acid; D006851:Hydrochloric Acid