Metabolism of 17-hydroxyprogesterone by a Bacillus species.
Fermentation of 17-hydroxyprogesterone with a Bacillus species (IICB-301) in a modified nutrient medium under aerobic conditions yielded androst-4-ene-3,17-dione and 15 alpha,17-dihydroxypregn-4-ene-3,20-dione in addition to a new pregnane analogue, 6 beta,17,20 alpha-trihydroxypregn-4-ene-3-one. Each microbial metabolite was characterized by the application of various spectroscopic techniques. The availability of the new metabolite, 6 beta,17,20 alpha-trihydroxypregn-4-ene-3-one, enabled complete elucidation of its 13C-n.m.r. spectrum.
D019326:17-alpha-Hydroxyprogesterone; D000735:Androstenedione / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D006908:Hydroxyprogesterones / Q000378:metabolism*; D013053:Spectrophotometry
D006908:Hydroxyprogesterones; C051326:6,17,20-trihydroxypregn-4-ene-3-one; D000735:Androstenedione; D019326:17-alpha-Hydroxyprogesterone; C044305:15,17-dihydroxyprogesterone