[Genetic transformation of somatic cells. X. Morphological transformation and changes in the growth characteristics of NIH 3T3 cells administered the oncogene v-myc].
Transfection of the plasmid containing the cloned gag-myc part of retrovirus MC 29 into mouse NIH 3T3 cells induces focuses of morphological transformation. Isolated morphological transformants have a decreased dependence on serum growth factors, a higher saturation density in monolayer, and an increased cloning efficiency on the glass and in agar. Induced traits are stably inherited, and may constitute the direct consequence of stable maintenance and expression of transfected oncogens.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000032:analysis / Q000648:ultrastructure*; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000032:analysis / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D051379:Mice; D009857:Oncogenes; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
D004247:DNA
Geneticheskaia transformatsiia somaticheskikh kletok. X. Morfologicheskaia transformatsiia i izmenenie rostovykh kharakteristik kletok NIH 3T3 pri vvedenii v nikh onkogena v-myc.