Time course of radiolabeled 2-deoxy-D-glucose 6-phosphate turnover in cerebral cortex of goats.
The in vivo dephosphorylation rate of 2-deoxy-D-glucose 6-phosphate (DGP) in the cerebral cortex of goats injected intravenously with radiolabeled 2-deoxy-D-glucose (DG) was investigated. Serial rapidly frozen samples of parietal cortical gray tissue were obtained at regular intervals over time periods from 45 min to 3 h in awake goats or in paralyzed and artificially ventilated goats maintained under 70% N2O or pentobarbital sodium anesthesia. The samples were analyzed for glucose content and separate DG and DGP activities. The rate parameters for phosphorylation (k3*) and dephosphorylation (k4*) were estimated in each animal. The glucose phosphorylation rate (PR) was calculated over the intervals 3-5 (or 6), 3-10, 3-20, 3-30, and 3-45 min, assuming k4* = 0. As the evaluation period was extended beyond 10 min, the calculated PR became increasingly less when compared with that calculated over the 3- to 5- (or 6) min interval (PRi). Furthermore, as metabolic activity decreased, the magnitude of the error increased such that at 45 min pentobarbital-anesthetized goats underestimated the PRi by 46.5% compared with only 23.1% in N2O-anesthetized goats. This was also reflected in the greater than twofold higher k4*/k3* ratio in the pentobarbital vs. N2O-anesthetized group. It is concluded that when using the DG method in the goat, DGP dephosphorylation cannot be ignored when employing greater than 10-min evaluation periods.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000378:metabolism*; D006041:Goats; D008433:Mathematics; D010766:Phosphorylation; D013997:Time Factors
D001786:Blood Glucose; D005958:Glucosephosphates; C015785:2-deoxyglucose-6-phosphate; D019298:Glucose-6-Phosphate; D003847:Deoxyglucose; D005947:Glucose