title: Glutathione reductase and glucose-6-phosphate dehydrogenase in erythrocytes treated with heavy metals.
abstract: Our previous study (Ribarov et al., 1982) indicated, that the level of reduced glutathione in erythrocytes treated with Ag+, Hg2+, Cu2+ and Pb2+ was decreased. In an attempt to shed light on this phenomenon in the present work glutathione reductase and glucose-6-phosphate dehydrogenase in erythrocytes incubated with heavy metals were investigated. The result obtained indicate that the glutathione reductase is significantly inactivated by Ag+, Hg2+ and Cu2+. The glucose-6-phosphate dehydrogenase activity was found to decrease only in erythrocytes incubated with Ag+ and Hg2+. No correlation between the inactivation of the enzymes tested and the decrease of the level of GSH was observed. A conclusion is made that the decrease of the level of GSH is not due to inactivation of the enzymes tested.
mesh_terms: D000818:Animals; D003300:Copper / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000097:blood; D005978:Glutathione / Q000097:blood; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors* / Q000097:blood; D006168:Guinea Pigs; D008628:Mercury / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology
chemical_list: D008670:Metals; D012834:Silver; D003300:Copper; D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase; D008628:Mercury; D005978:Glutathione