Partial reassembly of active 60S ribosomal subunits from rat liver following treatment with dimethylmaleic anhydride.
Rat liver 60S ribosomal subunits were treated with dimethylmaleic anhydride, a reagent for protein amino groups, at a 1/15,000 mol/mol ratio. This caused the dissociation of specific proteins, which were separated from the 56S residual core particles by centrifugation and identified by two-dimensional gel electrophoresis. The core particles lacking 30% of the total proteins retained most of the initial activity measured by the puromycin reaction but only small percentages of activities measured by polyphenylalanine synthesis, elongation-factor-2(EF-2)-dependent GTP hydrolysis and EF-2-mediated GDP binding. Upon reconstitution, the complementary amount of split proteins was incorporated into ribosomal particles, which had almost the same catalytic activities and biophysical properties (density, sedimentation coefficient and capability to reassociate to 40S subunits) as the original subunits.
D000818:Animals; D002384:Catalysis; D002458:Cell Fractionation / Q000379:methods; D002500:Centrifugation, Isopycnic; D004591:Electrophoresis, Polyacrylamide Gel; D005663:Furans; D008099:Liver / Q000648:ultrastructure*; D008299:Maleic Anhydrides; D012335:RNA, Ribosomal / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure*
D005663:Furans; D008299:Maleic Anhydrides; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C007474:2,3-dimethylmaleic anhydride