Glucose transport across plasma membrane in human platelets.
Studies have been carried out in the presence of 2-deoxyglucose, by utilizing a technique of platelet rapid filtration. Kinetic data suggest that glucose uptake across plasma membrane is the rate limiting step in its utilization. 2-deoxyglucose is transported by facilitated diffusion. L-glucose is transferred at only 1/1200 of the rate of glucose. Transport system shows high affinity for substrate. Transport is inhibited by cytochalasin B, phloretin and N-ethylmaleimide. Cytochalasin E does not affect 2-deoxyglucose uptake. Diamide can have activating or inhibitory effect. t-Butyl hydroperoxide is always activating. Insulin has no effect on rate transport. D-glucose, 3-O-methylglucose, non radioactive 2-deoxyglucose and D-mannose are strong competitors, whereas D-galactose and D-fructose compete weakly with 2-deoxyglucose transport.
D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010693:Phloretin / Q000494:pharmacology; D013329:Structure-Activity Relationship
D002250:Carbon Radioisotopes; D003571:Cytochalasin B; D003847:Deoxyglucose; D005947:Glucose; D005033:Ethylmaleimide; D010693:Phloretin