Are 'precipitated LDL' really low density lipoproteins?
Cholesterol, phospholipid and Apo B levels were determined in low density lipoproteins (LDL) precipitated by amphipathic polymers (Biomérieux kit, Marcy-l'Etoile, France) and compared to those of ultracentrifuged LDL. For 113 sera (triglyceride level less than 4.5 mmol/l, absence of very low density lipoproteins-remnants) LDL-cholesterol values were significantly higher (p less than 0.001), and those of LDL-phospholipids (p less than 0.001) and LDL-Apo B (p less than 0.005) significantly lower. The correlations between the 3 constituents studied gave statistically different regression coefficients for the two methods. It is likely that discrepancy in results is due to the effect of ultracentrifugation and precipitation removing from the lipoprotein spectrum different sections which nonetheless have some common physicochemical or immunological properties. It would seem that the term 'LDL' is inappropriate to designate these precipitated lipoproteins.
D001055:Apolipoproteins B / Q000097:blood; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D008077:Lipoproteins, LDL / Q000032:analysis*; D010743:Phospholipids / Q000097:blood; D014461:Ultracentrifugation
D001055:Apolipoproteins B; D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL; D010743:Phospholipids; D002784:Cholesterol