High-performance liquid chromatography and ultraviolet spectrophotometry for quantitation of tryptophan in barytic hydrolysates.
A procedure for quantitation of tryptophan in feedstuffs is described. It is based on barytic hydrolysis of material at 125 degrees C for 16 h, acidification of hydrolysate to pH 3 with HCl, high-performance liquid chromatography on Nova Pak C18 (Waters Assoc.), and spectrophotometric determination of tryptophan at 280 nm. The recovery of tryptophan from lysozyme added to samples ranges from 98.7 to 100%.
D001464:Barium; D017609:Barium Compounds; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D006868:Hydrolysis; D011506:Proteins / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000032:analysis*
D017609:Barium Compounds; D011506:Proteins; C012766:barium hydroxide; D001464:Barium; D014364:Tryptophan