Correcting errors in estimating neuron area caused by the position of the nucleolus.
The technique of measuring soma cross-sectional area at the plane of the nucleolus leads to systematic errors that depend on how far the nucleolus is displaced from the center of the soma. A set of correction factors was produced based on calculations from a geometric model of the measurement process. Applying the correction factors to measurements of second-order auditory neurons led to substantial changes in the estimated soma area.
D000818:Animals; D001306:Auditory Pathways / Q000166:cytology* / Q000502:physiology; D001933:Brain Stem / Q000166:cytology*; D002466:Cell Nucleolus / Q000648:ultrastructure; D002645:Chickens; D003051:Cochlea / Q000502:physiology; D003198:Computer Simulation; D008962:Models, Theoretical; D009474:Neurons / Q000166:cytology* / Q000648:ultrastructure