title: Pseudomonas infection of the biliary system resulting from use of a contaminated endoscope.
abstract: Pseudomonas aeruginosa was present in bile cultures from 10 patients who had undergone previous endoscopic retrograde cholangiopancreatography in 1984. After environmental cultures and review of instrument disinfection, we traced the infections to a single endoscope contaminated with P. aeruginosa, serotype 10. Although the instrument had been cleaned repeatedly with an automatic endoscope cleaning machine, P. aeruginosa survived on residual moisture left in the channels of the endoscope. Contamination ended only after we began to manually suction alcohol through the endoscope before air drying. In 5 of 10 patients, P. aeruginosa caused clinical infections including gangrenous cholecystitis, abscesses, and death. We could identify no factor that distinguished symptomatic from asymptomatic patients. In asymptomatic patients, P. aeruginosa was recovered from gallbladder bile up to 2 mo after endoscopic retrograde cholangiopancreatography. As this P. aeruginosa epidemic was discovered retrospectively because we monitor bile cultures, we advocate this practice as part of endoscopic retrograde cholangiopancreatography procedures.
mesh_terms: D001646:Bile / Q000382:microbiology; D001660:Biliary Tract Diseases / Q000635:transmission*; D002760:Cholangiopancreatography, Endoscopic Retrograde / Q000295:instrumentation*; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination; D006801:Humans; D011552:Pseudomonas Infections / Q000635:transmission*; D013242:Sterilization / Q000379:methods*