[Mechanism of the formation of DNA-protein cross-links during the gamma irradiation of chromatin in aqueous solutions].
The method of gel electrophoresis was used to study DNA-protein cross-link formation in fragmentized chromatin gamma-irradiated in water solutions (0.03%). By introducing changes into irradiation conditions (for instance, the use of different gases saturating the solution and the administration of radical acceptors) and by the subsequent electrophoretic analysis (treatment of the exposed chromatin by dissociating mixtures and enzymes) the authors showed a covalent nature of the cross-links in a radiation-induced DNA-protein complex and found the value of G (a cross-link) to be 0.02.
D002843:Chromatin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D004247:DNA / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D003720:Densitometry; D004586:Electrophoresis; D005720:Gamma Rays; D066298:In Vitro Techniques; D009690:Nucleic Acid Conformation / Q000187:drug effects / Q000528:radiation effects; D011487:Protein Conformation / Q000187:drug effects / Q000528:radiation effects; D011506:Proteins / Q000378:metabolism / Q000528:radiation effects*; D012996:Solutions
D002843:Chromatin; D011506:Proteins; D012996:Solutions; D004247:DNA
O mekhanizme obrazovaniia sshivok "DNK-belok" pri gamma-obluchenii khromatina v vodnykh rastvorakh.