Flavonoids as antioxidants evaluated by in vitro and in situ liver chemiluminescence.
Administration of eriodyctiol and (+)-catechin (10 mg/100 g of body weight) to mice inhibited the enhancement of in situ liver chemiluminescence produced by CCl4 (0.5 ml/100 g) by 32 and 38% respectively. 3,4-Dicaffeoylquinic acid was less effective (13%), and cynarin had no effect. Previously, these compounds and other polyphenols were assayed as in vitro antioxidants by their abilities to inhibit the tert-butyl hydroperoxide (t-BOOH)-initiated chemiluminescence of mouse liver homogenates, and the IC50 (microM) values were as follows: (+)-catechin, 3; eriodyctiol, 9; myricetin and 4,2',4'-trihydroxy-6'-metoxychalcone, 15; 3,4-dicaffeoylquinic acid, 20; isochlorogenic acid, 30; caffeic acid, 5,6,3'-trihydroxy-7,4'-dimethoxyflavone and cynarin, 50; chlorogenic acid and apigenin, 150; quercetin, pedalitin, sylimarin and quercetin-3-methyl ester, 200; 7,4'-dihydroxy-5-methoxyflavonone and kaempferol-3,7-dirhamnoside, 500; quercitrin, 900; and galangin-3-methyl ether, genkwanin, hesperidin, ombuoside, phloridzin, quinic acid, rhoifolin, rutin and sophoricoside, greater than 1 mM. The in vitro and in vivo effects of these flavonoids and polyphenols may be related to their antioxidant abilities, making them promising substances to be investigated as water-soluble protectors against lipid peroxidation and other free radical-mediated cell injury.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects*; D008163:Luminescent Measurements; D051379:Mice; D010636:Phenols / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000975:Antioxidants; D005419:Flavonoids; D008054:Lipid Peroxides; D010636:Phenols; D011108:Polymers; D002251:Carbon Tetrachloride