Comparison of immunological, biochemical and biophysical properties of three hemorrhagic factors isolated from the venom of Bothrops jararaca (jararaca).
Compared to the crude velonom of Bothrops jararaca, which needs 5000 ng to produce a hemorrhagic spot of 1 cm2 on rabbit skin, the isolated hemorrhagic factors HF1, HF2 and HF3 require 100, 20 and 15 ng of protein, respectively. Although these hemorrhagic factors possess different biochemical and biophysical properties, they are immunologically related proteins. The hemorrhagic, as well as the proteolytic, activities of these factors are destroyed by EDTA, acidic pH or heat treatments.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003435:Crotalid Venoms / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011817:Rabbits
D003435:Crotalid Venoms; C032814:hemorrhagin components (Crotalid)