Spectrophotometric determination of inorganic phosphate in the presence of thiol compounds.
A spectrophotometric method for inorganic phosphate determination in the presence of thiol compounds is described. Thiol compounds, which interfere with the measurement of inorganic phosphate by a modification of the method of Gomori, are removed by carboxymethylation by iodoacetate prior to the formation and reduction of phosphomolybdate complex. A linear standard curve is obtained by this method, and the method is suitable for the assay of a phosphate-releasing enzyme when the measurement must be performed in the presence of thiol compounds.
D010710:Phosphates / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis
D010710:Phosphates; D013438:Sulfhydryl Compounds