Production and characterization of antibodies against HT-2 toxin and T-2 tetraol tetraacetate.
Three new immunogens which were prepared by conjugation of the carboxymethyl oxime (CMO) derivatives of HT-2 toxin, T-2 tetraol (T-2 4ol), and T-2 tetraol tetraacetate (T-2 4Ac) to bovine serum albumin (BSA) were tested for the production of antibodies against the major metabolites of T-2 toxin. Antibodies against HT-2 toxin and T-2 4Ac were obtained from rabbits 5 to 10 weeks after immunizing the animals with CMO-HT-2-BSA and CMO-T-2 4Ac-BSA conjugates. Immunization with CMO-T-2 4ol-BSA resulted in no antibody against T-2 4ol. The antibody produced against HT-2 toxin had great affinity for HT-2 toxin as well as good cross-reactivity with T-2 toxin. The relative cross-reactivities of anti-HT-2 toxin antibody with HT-2 toxin, T-2 toxin, iso-T-2 toxin, acetyl-T-2 toxin, 3'-OH HT-2, 3'-OH T-2, T-2 triol, and 3'-OH acetyl-T-2, were 100, 25, 10, 3.3, 0.25, 0.15, 0.12 and 0.08%, respectively. Antibody against CMO-T-2 4Ac was very specific for T-2 4Ac and had less than 0.1% cross-reactivity with T-2 toxin, HT-2 toxin, acetyl-T-2 toxin, diacetoxyscirpenol, deoxynivalenol, and deoxynivalenol triacetate as compared with T-2 4Ac. The detection limits for HT-2 toxin and T-2 4ol by radioimmunoassay were approximately 0.1 and 0.5 ng per assay, respectively.
D000818:Animals; D000908:Antibodies, Fungal / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D003429:Cross Reactions; D005260:Female; D005670:Fusarium / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D012717:Sesquiterpenes / Q000276:immunology*; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000276:immunology*
D000908:Antibodies, Fungal; D012717:Sesquiterpenes; C046700:T-2 tetraol; D013605:T-2 Toxin; C012351:HT-2 toxin