Changes in cell surface charge and transmembrane potential accompanying neoplastic transformation of rat kidney cells.
Free flow electrophoresis measurements have been used to determine the surface charge density of normal rat kidney (NRK) cells and a clone of NRK, designated as 6m2, that exhibit a transformed phenotype at 33 degrees C and a non-transformed phenotype at 39 degrees C. A clone of 6m2, designated 54-5A4, which is transformed at both 33 degrees C and 39 degrees C was also studied. A surface charge density of -1.42 microC/cm2 was obtained for the NRK and non-transformed 6m2 cells at 39 degrees C, whereas at 33 degrees C values of -1.85 and -1.78 microC/cm2 were determined for the transformed 6m2 and 54-5A4 cells, respectively. It was found that 72% of the increased charge that appeared on the transformed 6m2 cells compared with the non-transformed 6m2 cells was RNAase sensitive. The time-dependent decrease in surface charge that accompanied the shift of the 6m2 cells from their transformed to non-transformed state was found to mirror the increase in transmembrane potential previously reported using a fluorescent dye technique, and was also comparable to the reported temporal changes in their morphology and virally-coded protein content.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D002471:Cell Transformation, Neoplastic; D004586:Electrophoresis; D004594:Electrophysiology; D007668:Kidney / Q000503:physiopathology*; D008564:Membrane Potentials; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D013696:Temperature
D012260:Ribonucleases