title: Intrinsic fluorescence studies on saccharide binding to Artocarpus integrifolia lectin.
abstract: The combining region of Artocarpus integrifolia lectin has been studied by using the ligand-induced changes in the fluorescence of the lectin. The saccharide binding properties of the lectin show that C-1, C-2, C-4, and C-6 hydroxyl groups of D-galactose are important loci for sugar binding. The alpha-anomer of galactose binds more strongly than its beta-counterpart. Inversion in the configuration at C-4 as in glucose results in a loss of binding to the lectin. The C-6 hydroxyl group is also presumably involved in binding as D-fucose does not bind to the lectin. The lectin binds to the Thomsen-Friedenreich antigen (Gal beta(1----3)GalNAc) more strongly than the other disaccharides studied, viz. Gal beta (1----4) Gal and Gal beta (1----3) GlcNAc, which are topographically similar to T-antigen. This observation suggests that the combining region of Artocarpus lectin is complementary to that of T-antigen. Solvent accessibility of the protein fluorophores have been probed by the quenching of protein fluorescence by Iodide ion in the absence and presence of sugar. In the presence of sugar a slight inaccessibility of the fluorophores to the solvent has been observed.
mesh_terms: D004187:Disaccharides; D005697:Galactosides; D006027:Glycosides; D007454:Iodides; D037102:Lectins; D008968:Molecular Conformation; D037121:Plant Lectins; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013816:Thermodynamics
chemical_list: D004187:Disaccharides; D005697:Galactosides; D006027:Glycosides; D007454:Iodides; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin