Polymorphism of EsD by isoelectric focusing: phenotyping in human tissues, dental pulps, hair roots, and semen.
The polymorphism of EsD was investigated in tissues of various human organs, dental pulps, hair roots, and seminal stains by isoelectric focusing. The method yielded an excellent resolution of the isoenzyme components. The time limits of determination were: in organ tissues 3 weeks, in dental pulps 1 week, and in hair roots several days. The 7-1 type was less stable than the common types. Phenotyping was possible from fresh semen samples, but was unsuccessful from dried seminal stains after storage. The results show that the EsD typing by isoelectric focusing is of practical use for medicolegal individualization of organs, teeth, and hairs.
D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D003782:Dental Pulp / Q000032:analysis; D006197:Hair / Q000032:analysis; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic; D012661:Semen / Q000032:analysis; D014018:Tissue Distribution; D014021:Tissue Preservation
D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human