Assessment of phospholipid deacylation-reacylation cycles by a stable isotope technique.
Incorporation of 18O into glycerophospholipids was determined after incubating mouse peritoneal exudate cells for 1 or 2 h in media containing 40% H(2)18O. Gas chromatography-mass spectrometry of hydrogenated fatty acid methyl esters showed highest amounts of 18O in choline phospholipids and phosphatidylinositol. Acyl groups generally present at the sn-1 position contained at least as much carbonyl 18O as those at the sn-2 position. Considering the route of 18O incorporation via free fatty acid derived through ester hydrolysis in H(2)18O, acyl turnover in certain peritoneal exudate cell phospholipids may equal or exceed 20% per h.
D000215:Acylation; D000818:Animals; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D010743:Phospholipids / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D005227:Fatty Acids; D010743:Phospholipids; D014867:Water