Activation of duck prothrombin by factor Xa and thrombin.
Prothrombin isolated from duck sodium citrate plasma was activated in a system containing duck factor Xa and calcium ions. Polyacrylamide gel electrophoresis showed that intermediates and the final product, thrombin, of Mr in the range 21 500-52 000 were present in the incubation mixture Serine and isoleucine were found to be the N-terminal amino acids of the intermediate form 1 and thrombin, respectively.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004372:Ducks / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005170:Factor X / Q000494:pharmacology*; D015951:Factor Xa; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D011516:Prothrombin / Q000097:blood*; D013917:Thrombin / Q000378:metabolism*
D010446:Peptide Fragments; D011516:Prothrombin; D005170:Factor X; D013917:Thrombin; D015951:Factor Xa; D002118:Calcium