[Characteristics of intracellular metabolism of carbohydrates and amino acids in animals with thiamine deficiency].
Vitamin B1 deficiency in mongrel albino rats induced by oxythiamine is concomitant with an increase in the ratio of free forms of NAD+/NADH, NADP+/NADPH in liver mitochondria. This is accompanied with a rise in steady-state concentrations of isocitrate, 2-oxoglutarate and with a decrease of malate in liver tissue, which testifies to the prominent regulatory role of the 2-oxoglutarate dehydrogenase complex in the intracellular metabolism under vitamin B1 deficiency. Moderate physical load causes a 10-fold increase in the steady-state concentration of pyruvate and lactate in the blood of thiamine-deficient animals, which seems to be due to the stimulation of glycolysis in the maintenance of energy homeostasis. The observed increase in the excretion of pyruvate, lactate, 2-oxoglutarate (30-fold against control) and pentose phosphates (3-fold) with urine, depending on the degree of vitamin B1 deficiency, points to one of essential mechanisms of cell metabolism stabilization under the given pathological condition.
D000096:Acetone / Q000097:blood; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D050260:Carbohydrate Metabolism; D002952:Citric Acid Cycle; D006019:Glycolysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010119:Oxythiamine / Q000494:pharmacology; D010427:Pentose Phosphate Pathway; D005082:Physical Exertion; D051381:Rats; D013832:Thiamine Deficiency / Q000378:metabolism*
D000596:Amino Acids; D009243:NAD; D010119:Oxythiamine; D000096:Acetone; D009249:NADP
Zakonomernosti vnutrikletochnogo metabolizma uglevodov i aminokislot u zhivotnykh pri nedostatochnosti tiamina.