CpA containing oligoribonucleotides specifically inhibit protein synthesis in rabbit reticulocytes.
The diribonucleoside monophosphate CpA (and no others) inhibits polypeptide chain elongation in rabbit reticulocyte lysates at 10-50 microM. Furthermore, all the trinucleotides containing CpA, i.e., XpCpA and CpApX (X = U, C, A or G) block polypeptide chain elongation as well. At 10 microM the inhibition by XpCpA and not CpApX is transient because a 3'-exonucleolytic activity destroys the critical CpA moiety. The inhibitors do not appear to interfere with the aminoacylation of tRNAs or disrupt the interaction of amino-acyl-tRNAs with the protein synthetic machinery. High levels (200 microM) of CpA or the trinucleotides containing CpA have no effect on translation in a wheat germ cell-free system.
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D003568:Cytidine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D003597:Cytosine Nucleotides / Q000494:pharmacology*; D015226:Dinucleoside Phosphates; D007700:Kinetics; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D013329:Structure-Activity Relationship
D003597:Cytosine Nucleotides; D015226:Dinucleoside Phosphates; D009843:Oligoribonucleotides; C013907:cytidylyl adenosine; D003568:Cytidine Monophosphate; D000241:Adenosine