Assessment of internal primary structure of polypeptides newly translated in vitro by reticulocyte lysate: a study with cytochrome b5.
A modified peptide mapping technique is described that allows the survey of the primary structure of proteolytic fragments of particular newly translated proteins in reticulocyte lysate. The technique is demonstrated with rat liver cytochrome b5.
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003573:Cytochrome b Group / Q000032:analysis*; D015786:Cytochromes b5; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D011233:Precipitin Tests; D051381:Rats; D012156:Reticulocytes / Q000201:enzymology*; D014357:Trypsin
D003573:Cytochrome b Group; D010455:Peptides; D015786:Cytochromes b5; D014357:Trypsin