Permeability of the blood-retinal barrier to carboxyfluorescein in eyes with rhegmatogenous retinal detachment.
Outward and inward permeability of carboxyfluorescein across the blood-retinal barrier were measured fluorophotometrically in seven cynomolgus monkey eyes with experimental rhegmatogenous retinal detachment. Probenecid was used to inhibit outward transport of carboxyfluorescein. The outward permeability was 1.98 +/- 0.31 microliter/min in eyes with retinal detachment and 0.84 +/- 0.15 microliter/min in control eyes with vitrectomy alone (P less than 0.01). The inward permeability, determined separately following intravenous injection, was significantly lower than the outward permeability: 0.14 +/- 0.02 microliter/min for eyes with retinal detachment and 0.04 +/- 0.01 microliter/min for control eyes. Since the outward permeability minus the inward permeability in the presence of probenecid represents that fraction of tracer moving due to fluid flow, it may be concluded that outward flow of fluid across the blood-retinal barrier is a substantial contributor to carboxyfluorescein loss from the vitreous cavity following intravitreal injection.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001813:Blood-Retinal Barrier / Q000187:drug effects*; D002199:Capillary Permeability; D005123:Eye / Q000378:metabolism; D005452:Fluoresceins / Q000097:blood*; D007275:Injections, Intravenous; D008252:Macaca fascicularis; D009994:Osmolar Concentration; D011339:Probenecid / Q000494:pharmacology; D012163:Retinal Detachment / Q000209:etiology / Q000378:metabolism*; D012167:Retinal Perforations / Q000150:complications*
D005452:Fluoresceins; C024098:6-carboxyfluorescein; D011339:Probenecid