title: Properties of the visual pigments of the moth Manduca sexta and the effects of two detergents, digitonin and chaps.
abstract: The three known visual pigments (P520, P450, P357) of the moth, Manduca sexta (Lepidoptera, Sphingidae), were extracted in two different detergents (2% digitonin, 6 or 12 mM CHAPS). As is the case in unextracted membranes, the metarhodopsins are quite stable in CHAPS extracts, while in digitonin the metarhodopsins of P520 and P450 decay rapidly at 15 degrees C to opsin and free retinal. The relative absorbance ratios are: 1.0:1.6 (P520:M485), 1.0:1.1 (P450:M485), and 1.0:0.8 (P357:M470). The relative amounts of the visual pigments found in digitonin extracts is 100:25:8 (P520:P450:P357); about 60 picomoles of P520 can be extracted from one Manduca retina.
mesh_terms: D000818:Animals; D002793:Cholic Acids; D003902:Detergents; D004072:Digitonin; D005260:Female; D007700:Kinetics; D007915:Lepidoptera / Q000032:analysis*; D008027:Light; D008297:Male; D009036:Moths / Q000032:analysis*; D012168:Retinal Pigments / Q000302:isolation & purification* / Q000528:radiation effects; D012172:Retinaldehyde; D012996:Solutions; D013053:Spectrophotometry; D013501:Surface-Active Agents
chemical_list: D002793:Cholic Acids; D003902:Detergents; D012168:Retinal Pigments; D012996:Solutions; D013501:Surface-Active Agents; D004072:Digitonin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate; D012172:Retinaldehyde