Location of T-cell leukaemia cells in a model rat system by means of a fluorescent probe.
Fluorescence probes for the active centre of an enzyme associated with tumour cells have been used to locate leukaemia cells in a model rat system. These fluorescent techniques are inexpensive and rapid to carry out. The leukaemic cells can be located by fluorescence microscopy in frozen sections, wax embedded sections and resin embedded sections. The technique is illustrated with reference to sections of leukaemic rat kidney, epididymis and testis. These studies confirm earlier histological findings employing conventional staining techniques and have the advantage that individual leukaemia cells can be detected in leukaemic animals undergoing drug therapy. The evidence suggests that these techniques will be of value in further studies of the design of drugs directed to leukaemia cells.
D000585:Aminacrine; D000818:Animals; D004822:Epididymis / Q000473:pathology; D005456:Fluorescent Dyes; D007680:Kidney Neoplasms / Q000175:diagnosis; D007942:Leukemia, Experimental / Q000175:diagnosis* / Q000188:drug therapy; D008214:Lymphocytes; D008297:Male; D011419:Propidium; D051381:Rats; D011919:Rats, Inbred Strains; D012074:Remission Induction; D013736:Testicular Neoplasms / Q000175:diagnosis; D014107:Tosyllysine Chloromethyl Ketone
D005456:Fluorescent Dyes; D014107:Tosyllysine Chloromethyl Ketone; D011419:Propidium; D000585:Aminacrine