Glutathione levels in cultured heart cells. Influence of buthionine sulfoximine, an inhibitor of glutathione synthesis.
In primary cultures of heart cells in mid-growth phase, levels of acid-soluble glutathione were 99 nmoles/mg protein and increased to 178 nmoles/mg protein at confluent growth. Glutathione disulfide accounted for less than 9% of the total. Levels of protein-bound mixed disulfide in mid-growth phase cells were 58 nmoles/mg protein and decreased to 36 nmoles/mg protein at confluent growth. Buthionine sulfoximine (BSO, 10(-4) M) depleted the levels of both glutathione and glutathione disulfide with no influence on the levels of protein-bound mixed disulfide. BSO had no influence on the multiplication rate of heart cells in primary culture. In secondary passage cultures, the levels of glutathione were less than half those of the primary cultures. BSO depressed cell growth and soluble glutathione, whereas the levels of protein-bound mixed disulfide were increased. These results showed that BSO depletes heart cells of soluble glutathione, whereas protein-bound thiol remains unchanged or increases.
D000818:Animals; D019328:Buthionine Sulfoximine; D002455:Cell Division; D002478:Cells, Cultured; D005978:Glutathione / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019803:Glutathione Disulfide; D006321:Heart / Q000187:drug effects; D007700:Kinetics; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D005978:Glutathione; D019803:Glutathione Disulfide