Pseudomonas diminuta LPS with a new endotoxic lipid A structure.
Lipid A that contains mainly 2,3-diamino-2,3-dideoxy-D-glucose, phosphate and fatty acids in the molar ratio 2:1:5-6 was found in Pseudomonas diminuta lipopolysaccharide. The lipid A was considered to have a diamino-sugar disaccharide structure that carries a nonglycosidic phosphomonoester group and amide-bound acyloxyacyl and 3-hydroxy fatty acyl groups. The lipopolysaccharide exhibited endotoxic activities including lethal toxicity, pyrogenicity, local Shwartzman activity, body weight-decreasing toxicity and Limulus activity. The free lipid A was also endotoxic.
D000818:Animals; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D005944:Glucosamine / Q000031:analogs & derivatives / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D008050:Lipid A / Q000302:isolation & purification*; D008070:Lipopolysaccharides / Q000302:isolation & purification* / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D011549:Pseudomonas / Q000032:analysis*; D013400:Sugar Acids / Q000032:analysis
D005227:Fatty Acids; D006539:Heptoses; D008050:Lipid A; D008070:Lipopolysaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; C040138:2,3-diamino-2,3-dideoxyglucose; D005947:Glucose; D005944:Glucosamine