In vitro sensitivity of human ovarian tumors to chemotherapeutic agents with adjuvant danazol.
Human ovarian tumors have been cultured as primary monolayers in vitro. The in vitro chemosensitivity was determined indirectly using [3H]leucine uptake with cisplatinum, adriamycin, bleomycin, thiotepa, and chlorambucil. Chemosensitivity also was determined using these agents in the presence of danazol. The tumor cultures showed a uniform increase in sensitivity to these agents in the presence of danazol, suggesting that this drug might merit testing as an adjuvant in the chemotherapy of ovarian cancer.
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D003613:Danazol / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011245:Pregnadienes / Q000627:therapeutic use*; D014176:Protein Biosynthesis; D014410:Tumor Stem Cell Assay
D011245:Pregnadienes; D007930:Leucine; D003613:Danazol