A specific radioimmunoassay for the detection of 19-nortestosterone residues in urine and plasma of cattle.
19-Nortestosterone (Nandrolone) is illegally used as a growth promoter for meat producing animals. This work describes a radio-immunoassay (RIA) to detect, in cattle urine, Nandrolone and its metabolites. The antiserum was specific for 19-nortestosterone and its main metabolites, and to a lesser extent for Trenbolone (17 beta-hydroxyestra-4,9,11-trien-3-one). The sensitivity of this assay was 6 ug/l at 90 % B0/T binding. This assay can be used in forensic control of illegal treatment with 19-nortestosterone in meat production.
D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D008832:Microchemistry; D009277:Nandrolone / Q000097:blood* / Q000378:metabolism / Q000652:urine; D011863:Radioimmunoassay / Q000379:methods
D009277:Nandrolone