ATP measurements obtained by luminometry provide rapid estimation of Ureaplasma urealyticum growth.
ATP content obtained by luciferin-luciferase luminometry with commercially available reagents provided rapid estimates of Ureaplasma urealyticum populations. Each cell contained about 4.7 X 10(-18) mol of ATP. We could detect 10(4) CCU50 (color change unit50) per 100 microliters. We correlated urease activity with growth and confirmed the differential response of ureaplasma strains to Mn2+.
D000255:Adenosine Triphosphate / Q000032:analysis*; D049454:Firefly Luciferin; D008156:Luciferases; D008345:Manganese / Q000494:pharmacology; D014509:Ureaplasma / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D014510:Urease / Q000378:metabolism
D008345:Manganese; D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases; D014510:Urease