Studies on the effect of inflammation on the synthesis of glucosylated intermediates of glycoprotein biosynthesis in rat liver microsomes.
Glycoprotein biosynthesis is substantially increased in liver during experimental inflammation. This study describes the effect of inflammation on the incorporation of labelled Glc from UDP-Glc into glucosylated lipid-linked intermediates of glycoprotein biosynthesis in liver microsomes. Maximum incorporation of Glc into lipid sugar and lipid oligosaccharide fractions occurred using microsome fractions from rats suffering from inflammation for 48-72 hr. Incorporation of Glc into lipid-sugar fractions was increased about three-fold over controls and incorporation into lipid-oligosaccharide fractions was increased about four-fold compared to controls. Maximum incorporation of Glc into lipid-sugar and lipid-oligosaccharide occurred at pH 6.0 and pH 5.2, respectively.
D000818:Animals; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007249:Inflammation / Q000378:metabolism*; D050356:Lipid Metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D051381:Rats
D006023:Glycoproteins; D009844:Oligosaccharides