Serotonin induced actin polymerization and association with cytoskeletal elements in cultured bovine aortic endothelium.
Serotonin, (5-hydroxytryptamine, 5-HT) binds to cultured endothelial cell stress fibers as identified by fluorescence microscopy and in vitro induces actin polymerization as measured by DNase 1 inhibition and differential centrifugation; the structural change in actin in the presence of 5-HT resembles actin paracrystals as visualized by negative staining under electron microscopy. These observations indicate that 5-HT, which is taken up by endothelium by a non-mediated diffusion, may interact directly with actin to affect cytoskeletal changes.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000648:ultrastructure; D002417:Cattle; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004727:Endothelium / Q000378:metabolism / Q000648:ultrastructure; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D012701:Serotonin / Q000494:pharmacology*
D000199:Actins; D046911:Macromolecular Substances; D012701:Serotonin