Human tissue fibronectin: expression of different isotypes in the adult and fetal tissues.
The isotype of fibronectin present in human adult and fetal lung tissues was studied by limited proteolysis by cathepsin D followed by immunoblot analysis with domain-specific antibodies. The results indicate that the fibronectin in the adult lung tissue is predominantly the plasma-type whereas the fibronectin in the fetal lung tissue is more related to the cellular-type than to the plasma-type. Thus, it appears that the fibronectin isotype in tissue switches from the cellular-type to the plasma-type during ontogenesis.
D000328:Adult; D000911:Antibodies, Monoclonal; D002402:Cathepsin D / Q000378:metabolism; D005333:Fetus / Q000032:analysis*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007159:Immunologic Tests; D008168:Lung / Q000032:analysis* / Q000196:embryology; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D010446:Peptide Fragments; D002402:Cathepsin D