title: Location of two cysteines in brain beta 1-tubulin that can be cross-linked after removal of exchangeable GTP.
abstract: Two cysteines of the major neuronal beta-tubulin subunit (beta 1) can be specifically cross-linked with the bifunctional sulfhydryl reagent N',N'-ethylenebis(iodoacetamide) after removal of GTP. A cysteine in position 12 cross-links with one of the cysteines in position 201 or 211. Although the two cross-linked cysteines are separated by at least 189 residues in the primary structure of tubulin, they are maximally 9 A apart in the tertiary structure.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain; D002417:Cattle; D003432:Cross-Linking Reagents; D003545:Cysteine; D005029:Ethylenediamines; D006160:Guanosine Triphosphate / Q000378:metabolism; D010446:Peptide Fragments; D014404:Tubulin / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D005029:Ethylenediamines; D010446:Peptide Fragments; D014404:Tubulin; C032156:N,N'-ethylenebis(iodoacetamide); D006160:Guanosine Triphosphate; D003545:Cysteine