Isolation and primary structure determination of amphibian skin tryptophyllins.
A large new set of peptides has been isolated from the skin of Phyllomedusa rohdei, a South American frog. All these peptides contain a tryptophyl residue, which justified the suggested denomination of tryptophyllins (TPHs). On the basis of their amino acid chain length, they have been classified as TPH-4, TPH-5, TPH-7 and TPH-13. With the exception of TPH-13 these peptides seem to possess an action on liver protein synthesis and body weight; in addition TPH-7 and TPH-13 show endocrine and behavioural effects. The methodologies employed in purification and structure determination of TPHs will be illustrated.
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000502:physiology*; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D007526:Isoelectric Point; D013058:Mass Spectrometry; D009842:Oligopeptides / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D012867:Skin / Q000032:analysis
D009842:Oligopeptides; D010446:Peptide Fragments; C048291:tryptophyllins