Estrogen receptors in stromal and epithelial fractions of the ventral prostate of rats.
The cytosol from rat ventral prostates contains two estrogen-binding components that can be separated in a glycerol gradient: a high-affinity (KD = 4.2 X 10(-10) M), low-capacity (110 fmole/mg DNA) species that runs in the 8S region of the gradient, and a lower-affinity (KD = 2.4 X 10(-9) M), higher-capacity (410 fmole/mg DNA) 4S protein. Separation of prostate tissue into stromal- and epithelial-enriched fractions was associated with the disappearance of the latter component. When compared per milligram DNA stromal- and epithelial-enriched fractions contained similar amounts of the high-affinity 8S-binding protein. In vivo estrogen treatment caused a time-related disappearance of the protein from the intact cytosol presumably as a result of translocation. No major differences in the degree of binding to the high-affinity component were observed in rats of different ages. Estrogens seem to exert direct effects on both stromal and epithelial tissues of the prostate.
D000375:Aging; D000818:Animals; D003238:Connective Tissue / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D008297:Male; D011467:Prostate / Q000032:analysis / Q000166:cytology / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D011959:Receptors, Estradiol / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis*; D013997:Time Factors; D014599:Uterus / Q000166:cytology
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D004958:Estradiol