The effect of adrenalectomy on the proteinuria of spontaneously hypertensive rats and normotensive controls.
Urinary proteins were studied by quantitative and electrophoretic methods in 6-month-old spontaneously hypertensive rats and normotensive controls. Protein analysis was carried out before and after adrenalectomy and during gluco- or mineralocorticoid treatment. Urinary protein excretion was significantly diminished after adrenalectomy both in the hypertensive and control groups. The original level of protein excretion was restored only by glycocorticoid treatment. Normal or pathologic electrophoretic pattern of urinary proteins was not influenced by the experimental procedure. Moderately non-selective glomerular proteinuria persisted in the spontaneously hypertensive rats referring to a definitely damaged glomerular barrier. One protein fraction of about 130,000 dalton molecular weight disappeared from the urine of hypertensive animals after adrenalectomy and reappeared after glucocorticoid treatment only. This fraction probably represents the dimeric form of albumin. Quantitative changes of urinary protein excretion can be explained by haemodynamic factors.
D000305:Adrenal Cortex Hormones / Q000502:physiology; D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D006973:Hypertension / Q000150:complications*; D007678:Kidney Glomerulus / Q000473:pathology; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification; D011507:Proteinuria / Q000209:etiology* / Q000473:pathology / Q000652:urine; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D000305:Adrenal Cortex Hormones; D011506:Proteins