Macrophage cell line, J774, producing a tumor necrosis factor.
Cytotoxic factor was produced from murine macrophage-like cell line, J774, after stimulation with 12-o-tetradecanoyl phorbol-13-acetate (TPA) and lipopolysaccharide (LPS). J774-derived cytotoxic factor is a glycoprotein with a molecular weight of 39,000 by gel filtration and 18,000 by SDS-PAGE, and with an isoelectric point of below 4.3. J774-derived cytotoxic factor exhibited cytotoxicity to L(S) cells, not cytotoxic to L(R) cells, and a necrotizing action against transplanted Meth A sarcoma. J774-derived cytotoxic factor and murine serum tumor necrosis factor (TNF) were identical as regards properties.
D000818:Animals; D002460:Cell Line; D003603:Cytotoxins / Q000096:biosynthesis / Q000302:isolation & purification; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha
D003603:Cytotoxins; D006023:Glycoproteins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D013755:Tetradecanoylphorbol Acetate