The comparative study of cardiovascular toxins utilizing a sensitive indicator of sublethal toxic injury.
This study measures the inhibition of [3H]uridine uptake by smooth muscle cells to determine sublethal toxic injury by the cardiovascular toxins, allylamine, isoproterenol, and beta-aminopropionitrile. The exposure period or the concentration of toxin which inhibited 30% of [3H]uridine uptake by smooth muscle cells could be utilized as an endpoint for ranking toxicity. Cytotoxicity of the three toxins to smooth muscle cells were ranked as: allylamine greater than isoproterenol greater than beta-aminopropionitrile. Recovery of cells utilizing [3H]uridine uptake inhibition as a method for assessing comparative cytotoxicity and for screening of agents potentially injurious to vascular cells.
D000499:Allylamine / Q000494:pharmacology / Q000633:toxicity*; D000588:Amines / Q000633:toxicity*; D000629:Aminopropionitrile / Q000494:pharmacology / Q000633:toxicity*; D000818:Animals; D001013:Aorta, Thoracic; D002318:Cardiovascular Diseases / Q000139:chemically induced*; D007545:Isoproterenol / Q000494:pharmacology / Q000633:toxicity*; D007700:Kinetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D013552:Swine; D014529:Uridine / Q000378:metabolism
D000588:Amines; D000629:Aminopropionitrile; D000499:Allylamine; D007545:Isoproterenol; D014529:Uridine