Determination of the molecular mass of apolipoprotein B-100. A chemical approach.
Apolipoprotein B-100 (apo B-100) is the protein ligand in low-density lipoproteins that binds to a specific cell-surface receptor. Its molecular mass has been a subject of controversy. We have determined the molecular mass of the protein by a chemical approach. After complete CNBr cleavage, the C-terminal fragment of apo B-100 was purified by reverse-phase h.p.l.c. Amino acid N- and C-terminal analyses confirm that this peptide represents the C-terminal peptide as deduced from the DNA sequence of a human apo B-100 cDNA clone. A chemically synthesized peptide was used to determine the recovery of the peptide (74.72%). On the basis of these data, the molecular mass of apo B-100 was determined to be 496.82 +/- 24.84 kDa.
D000595:Amino Acid Sequence; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D010446:Peptide Fragments; D003488:Cyanogen Bromide