title: In vitro sensitivity of human ovarian tumors to chemotherapeutic agents with adjuvant danazol.
abstract: Human ovarian tumors have been cultured as primary monolayers in vitro. The in vitro chemosensitivity was determined indirectly using [3H]leucine uptake with cisplatinum, adriamycin, bleomycin, thiotepa, and chlorambucil. Chemosensitivity also was determined using these agents in the presence of danazol. The tumor cultures showed a uniform increase in sensitivity to these agents in the presence of danazol, suggesting that this drug might merit testing as an adjuvant in the chemotherapy of ovarian cancer.
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D003613:Danazol / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011245:Pregnadienes / Q000627:therapeutic use*; D014176:Protein Biosynthesis; D014410:Tumor Stem Cell Assay
chemical_list: D011245:Pregnadienes; D007930:Leucine; D003613:Danazol