The quantitative separation of chylomicrons and chylomicron remnants by column chromatography.
Chylomicrons and chylomicron remnants cannot be separated by classical techniques of ultracentrifugation or gel filtration, since there is a marked overlap of density and size. Chylomicron remnants develop a high negative surface charge during their formation presumably due to surface-oriented free fatty acids. Two chromatographic matrices have been identified which separate these two lipoprotein species based on the charge differences. Both DEAE-Sephacel and protamine-Affi-Gel 10 effect a quantitative separation. These techniques may be useful in studies of chylomicron metabolism both in vivo and in vitro.
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002914:Chylomicrons / Q000302:isolation & purification*; D005230:Fatty Acids, Nonesterified; D008297:Male; D051381:Rats; D014461:Ultracentrifugation
D002914:Chylomicrons; D005230:Fatty Acids, Nonesterified