title: Homology between the ran1+ gene of fission yeast and protein kinases.
abstract: The ran1+ gene of the fission yeast Schizosaccharomyces pombe is a negative regulator of both sexual conjugation and meiosis. The nucleotide sequence of the gene has been determined and contains a region of open reading frame (ORF) capable of encoding a protein of 52,000 daltons. S1 nuclease analysis of ran1+-encoded RNA showed that the ORF was spanned by an uninterrupted transcript. A fragment of DNA containing the entire ran1+ gene was expressed in a bacterial expression vector and found to encode the expected product of 52,000 daltons. The putative ran1+ gene product shares significant sequence homology with known protein kinases. The level of the ran1+ transcript was similar in vegetative and meiotic cells suggesting that the ran1+ protein product rather than its transcript is regulated during sexual differentiation.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005796:Genes; D005800:Genes, Fungal; D008970:Molecular Weight; D011494:Protein Kinases / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D011494:Protein Kinases