title: Ornithine decarboxylase activity associated with a particulate fraction of brain.
abstract: The enzymic decarboxylation of ornithine by adult rat brain largely occurs in the particulate fraction. The activity is primarily due to ornithine decarboxylase (ODC) as evidenced by several criteria: the concurrent production of equimolar amounts of CO2 and putrescine, the sensitivity of the reaction to difluoromethylornithine (DFMO), a specific inhibitor of ODC, the lack of major effect of two inhibitors of ornithine-2-oxo-acid transaminase, upon the DFMO-sensitive component of decarboxylation, the failure to profoundly reduce decarboxylation activity in the presence of a large excess of many aminoacids which could compete for non-specific decarboxylases. The insoluble ODC activity appears largely within synaptosomal and mitochondrial-enriched morphological fractions, yet cannot be attributed to trapped soluble ODC. Particulate ODC has a pH optimum and kinetic parameters that differ from those of soluble cerebral ODC.
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002384:Catalysis; D003653:Decarboxylation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009952:Ornithine / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D009952:Ornithine; D009955:Ornithine Decarboxylase