Identification of the cooked food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and its N-acetylated and 3-N-demethylated metabolites in rat urine.
The extractable unconjugated metabolites of 2-amino-3-methylimidazo-[4,5-f]quinoline (IQ) were identified in the urine of rats which had received a single dose of [3H]IQ by gavage. The dichloromethane and ethylacetate extracts prepared from the alkalinized 0-24 h urines were analyzed by radio-TLC and radio-HPLC. The 3 major HPLC fractions were submitted to DCI-MS analysis. Unchanged IQ, N-acetylIQ and 3-N-demethylIQ have been identified. Extractable unconjugated metabolites represent only about 0.85% of the total radioactivity excreted in the urine.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003296:Cooking; D005506:Food Contamination; D008297:Male; D009153:Mutagens / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D009153:Mutagens; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline