Role of hydrocortisone, chloroquine and prednisolone in neutrophil mediated cytotoxicity.
Human polymorphonuclear leukocytes (PMNs) obtained from normal donors kill tumor cells in vitro. This system was utilized to study the role of lysosomal enzyme release in the cytotoxicity of human neutrophils. When the neutrophils were incubated with hydrocortisone, chloroquine and prednisolone, which are drugs known to stabilize lysosomal membranes, hydrocortisone and chloroquine were unable to inhibit cytotoxicity whereas treatment with prednisolone inhibited cytotoxicity. Recovery in cytotoxicity was observed as the dose of prednisolone utilized was decreased. Cytotoxicity equivalent to that observed with no added drug was found 5 X 10(-7) M for prednisolone. Pretreatment of neutrophils with a dose of prednisolone known to inhibit cytotoxicity (5 X 10(-6) M) resulted in decreased release of the lysosomal enzyme, acid phosphatase, in comparison to untreated neutrophils.
D000135:Acid Phosphatase / Q000378:metabolism; D002738:Chloroquine / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D066298:In Vitro Techniques; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D011239:Prednisolone / Q000494:pharmacology*
D002738:Chloroquine; D011239:Prednisolone; D000135:Acid Phosphatase; D006854:Hydrocortisone