Differences in the uptake of modified low density lipoproteins by tissue cultured endothelial cells.
Acetylated low density lipoprotein (Ac-LDL) is taken up by bovine aortic and adrenal capillary cells but not by brain capillary cells. This indicates that the uptake of Ac-LDL is not a feature of all types of endothelial cell. A quantitative examination of lipoprotein uptake by flow cytometry showed cells in G2M took up 45% more Ac-LDL than those in G1S. Compared with confluent cultures, sub-confluent bovine aortic cells took up three times as much LDL but Ac-LDL uptake was increased by only 34%. This indicates that the Ac-LDL receptor is not down-regulated to the same extent as that for LDL.
D000107:Acetylation; D000311:Adrenal Glands / Q000166:cytology; D000818:Animals; D001011:Aorta / Q000166:cytology; D001921:Brain / Q000166:cytology; D002417:Cattle; D002452:Cell Count; D002453:Cell Cycle; D002469:Cell Separation; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism
D008077:Lipoproteins, LDL