A flow cytofluorometric double staining technique for simultaneous determination of human mononuclear cell surface phenotype and cell cycle phase.
A double staining technique for the simultaneous determination by flow cytofluorometry of cell surface phenotype and cell cycle phase is described. Peripheral blood mononuclear cells were stained with fluorescein-conjugated monoclonal antibodies for cell surface phenotype, fixed serially with 2% paraformaldehyde and 71.25% ethanol, and stained with propidium iodide to label cellular DNA. The cells were then analyzed by flow cytofluorometry for both green and red fluorescence. A variety of cells, including T cells and their subsets, B cells, NK cells and monocyte/macrophages, can be identified by this technique with simultaneous determination of cell cycle phase.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002453:Cell Cycle; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D019793:Fluorescein; D005452:Fluoresceins; D006801:Humans; D007962:Leukocytes / Q000145:classification; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005452:Fluoresceins; D004247:DNA; D019793:Fluorescein