Dye-mediated photolysis of normal and neoplastic hematopoietic cells.
The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by greater than or equal to 4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.
D001854:Bone Marrow Cells; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells; D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000473:pathology*; D010782:Photolysis; D011744:Pyrimidinones; D013997:Time Factors
D011744:Pyrimidinones; C003954:merocyanine dye