The preparation of 99mTc-tertiarybutylisonitrile (99mTc-TBI) by a method suitable for routine clinical use.
The myocardial imaging agent technetium-99m-hexakis (tertiarybutylisonitrile) (99mTc-TBI) was prepared by the reaction of [99mTc]pertechnetate with TBI in 50% ethanol/0.9% saline at 100 degrees C, using stannous chloride as the reducing agent. A study of the reaction parameters enabled the yield to be optimized to better than 90%, although this was reduced to approximately 60% if a purification step was carried out. Chromatographic analysis on ITLC-SG medium showed the final product to be of high radiochemical purity. Biological studies comprising biodistribution in mice over a 2-h period, imaging studies in animals and sub-acute toxicity testing in mice indicated that 99mTc-TBI prepared as here described is a suitable agent for routine clinical use in humans.
D000818:Animals; D005260:Female; D006321:Heart / Q000000981:diagnostic imaging*; D007202:Indicators and Reagents; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009570:Nitriles / Q000138:chemical synthesis* / Q000633:toxicity; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000633:toxicity; D015609:Organotechnetium Compounds; D011817:Rabbits; D011877:Radionuclide Imaging; D013552:Swine; D013667:Technetium; D014018:Tissue Distribution
D007202:Indicators and Reagents; D009570:Nitriles; D009942:Organometallic Compounds; D015609:Organotechnetium Compounds; C043714:technetium Tc 99m hexakis(tert-butylisonitrile); D013667:Technetium