title: A sensitive procedure for quantifying the phagocytic competence of blood granulocytes.
abstract: The phagocytic activity of blood granulocytes can be quantitatively assayed by ingestion of opsonised paraffin oil droplets containing the dye "Oil Red-O" (Stossel, T.P., Mason, R.J., Hartwig, J., and Vaughan, M. (1972) J. Clin. Invest. 51: 615-624). We have modified this assay by incorporating [3H]glycerol into the oil droplets which allows a more sensitive and reproducible measurement of the phagocytic competence of blood granulocytes even at very low cell counts. Comparative studies after one day storage of the blood at 4 degrees C is feasible since they retain 84% of the phagocytic capacity measured when isolated from fresh blood.
mesh_terms: D000818:Animals; D001391:Azo Compounds; D005990:Glycerol; D006098:Granulocytes / Q000502:physiology*; D066298:In Vitro Techniques; D007700:Kinetics; D010587:Phagocytosis; D013552:Swine
chemical_list: D001391:Azo Compounds; C011049:oil red O; D005990:Glycerol