Fluctuation domains in myoglobin. Fluorescence quenching studies.
The dynamics of two domains in the myoglobin molecule, close to the heme and inside the protein medium including the surface, are investigated through the study of the fluorescence oxygen quenching of two probes imbedded in the heme pocket: zinc protoporphyrin IX (with a fluorescence lifetime of 2.1 ns) and metal-free protoporphyrin IX (with a fluorescence lifetime of 17.8 ns).
D006418:Heme / Q000032:analysis; D009211:Myoglobin / Q000032:analysis*; D010100:Oxygen; D011524:Protoporphyrins / Q000032:analysis; D013050:Spectrometry, Fluorescence
D009211:Myoglobin; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D006418:Heme; C028025:protoporphyrin IX; D010100:Oxygen