Expression of Agrobacterium tumefaciens T-DNA gene 7 in Xenopus laevis oocytes.
The expression of Agrobacterium tumefaciens T-DNA gene 7 was investigated in Xenopus laevis oocytes. Cloned DNA injected into oocytes consisted of T-DNA sequences derived from octopine type Ti plasmid B6-806 and T-DNA attached to plant DNA sequences at the left junction in crown gall tumors. Transcription initiation sites observed in oocytes were similar to those for transcript 7 in crown gall tumors. Quantitative differences in transcription occurred depending on the flanking sequences of the injected clones indicating that sequences upstream of the TATA box of T-DNA gene 7 affect the quantitative expression of this gene in Xenopus oocytes.
D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism*; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D010941:Plant Tumors; D010944:Plants / Q000235:genetics; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014982:Xenopus laevis / Q000235:genetics*
D004269:DNA, Bacterial; D004274:DNA, Recombinant