Genetic organization of the c-sis transcription unit.
The structure and genetic organization of the transcription unit of the c-sis proto-oncogene was determined. Comparative nucleotide sequence analysis of the exon sequences of feline and human c-sis revealed a very high degree of homology. The cap site as well as the poly(A)-addition site of the sis transcript of each species was identified and found in similar positions. An insert of 4 amino acids was found in the deduced translational product of feline c-sis and it was located at the amino-terminus of the region that constituted the platelet-derived growth factor domain. An insert of 149 bp present at the 5' end of exon 7 of human c-sis but absent in the simian sarcoma virus v-sis oncogene was also present in the feline c-sis proto-oncogene.
D000818:Animals; D001483:Base Sequence; D002415:Cats; D003001:Cloning, Molecular; D003360:Cosmids; D004274:DNA, Recombinant / Q000378:metabolism; D005091:Exons; D005809:Genes, Regulator; D006801:Humans; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D014176:Protein Biosynthesis; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012315:RNA Caps; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic
D004274:DNA, Recombinant; C000715470:MAS1 protein, human; D010982:Platelet-Derived Growth Factor; D000090063:Proto-Oncogene Mas; D012315:RNA Caps