Effects of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) on the levels of glutathione and lipid peroxidation and the activity of glutathione reductase in liver and lung.
After subcutaneous injection of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) to rats, glutathione reductase activity in lung and liver diminished rapidly. The restoration of enzyme activity occurred more slowly in the lung than in the liver. The pattern for the time-course of total glutathione (GSH) levels was similar between lung and liver, except for a marked depression of hepatic levels 6 h after drug administration. The level of malondialdehyde (MDA) in lung was not affected by BCNU throughout the experimental period (3 days). However, the level in liver had increased significantly by 6 h after drug administration. These observations indicate that lipid peroxidation in lung was not induced by BCNU even when glutathione reductase activity was markedly diminished. In contrast, the lipid peroxidation in liver was induced by BCNU and was preceded by an early marked depression in total GSH.
D000818:Animals; D002330:Carmustine / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis*; D005980:Glutathione Reductase / Q000378:metabolism*; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008099:Liver / Q000032:analysis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008168:Lung / Q000032:analysis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
D008054:Lipid Peroxides; D005980:Glutathione Reductase; D005978:Glutathione; D002330:Carmustine