title: Expression of lectin-binding surface glycoproteins during the development of Schistosoma mansoni schistosomula.
abstract: Tegumental glycoproteins of Schistosoma mansoni cercariae, mechanically produced 24-hr and 48-hr schistosomula, and adult worms were radioiodinated with the Bolton-Hunter reagent, then isolated by lectin affinity chromatography. SDS-PAGE revealed Con A binding glycoproteins with apparent molecular weights of 180,000, 150,000, 43,000, and 30,000 in detergent extracts of the tegument of cercariae. These glycoproteins are retained by 24-hr mechanically produced, cultured schistosomula and are accompanied by the appearance of 2 additional labeled glycoproteins, mol. wt. 66,000 and 57,000. In 48-hr schistosomula, there is a marked increase in the relative size of the 66,000 mol. wt. peak. In contrast, the 57,000 mol. wt. glycoprotein is the major radiolabeled Con A binding component of the adult tegument; the other peaks are either reduced or absent in adults. Similar findings were obtained following affinity chromatography using immobilized Lens culinaris lectin or Ricinus communis agglutinin, and following metabolic labeling of glycoproteins with tritiated galactose.
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008970:Molecular Weight; D037121:Plant Lectins; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D000954:Antigens, Surface; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; C033619:lentil lectin; D003208:Concanavalin A