[New method for the continuous determination of the catalytic activity of pancreatic carboxylesterase].
A new method is reported for the continuous determination of the catalytic activity of pancreatic carboxyl esterase. 1-Naphthyl butyrate is employed as the substrate. The released 1-naphthol is coupled with a new colour reagent, 2,6-dichloroquinone-4-chloroimide. The intensity of the resulting colour is directly proportional to the enzyme activity. Preliminary reference values for serum and pancreatic secretion are reported.
D002265:Carboxylic Ester Hydrolases / Q000097:blood / Q000378:metabolism*; D004796:Clinical Enzyme Tests; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D010179:Pancreas / Q000201:enzymology*; D010182:Pancreatic Diseases / Q000175:diagnosis; D013053:Spectrophotometry / Q000379:methods
D007202:Indicators and Reagents; D002265:Carboxylic Ester Hydrolases
Eine neue Methode zur kontinuierlichen Bestimmung der katalytischen Aktivität der Pankreas-Carboxyl-Esterase.