Effect of pyridoxine deficiency on ornithine aminotransferase in rat kidney and liver.
The hepatic and renal activities of ornithine aminotransferase (OAT) [L-ornithine 2-oxoacid aminotransferase, EC 2.6.1.13] were determined in male and female rats given diet with or without pyridoxine. OAT activities were measured in the presence of pyridoxal phosphate. Diet without pyridoxine caused a decrease in hepatic OAT activity in males and no change in females and an increase in renal OAT activity in males and a decrease in females. The increased renal OAT activity in males was associated with increased immunochemically recognizable OAT, and was due to increased synthesis of OAT as shown by measurement of [3H]leucine incorporation in vivo. Thus OAT is regulated by nutritional conditions in different ways in liver and kidney.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D005260:Female; D007668:Kidney / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D009953:Ornithine-Oxo-Acid Transaminase / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors; D000637:Transaminases / Q000378:metabolism*; D026681:Vitamin B 6 Deficiency / Q000201:enzymology*
D000954:Antigens, Surface; D008565:Membrane Proteins; D011506:Proteins; D000637:Transaminases; D009953:Ornithine-Oxo-Acid Transaminase