title: Determination of cyclopiazonic acid in peanuts and corn by thin layer chromatography.
abstract: A thin layer chromatographic system including densitometry has been developed for determining cyclopiazonic acid in peanuts and corn. Samples are extracted with methanol-chloroform (20 + 80); the extract is stripped of most interferences by partitioning with aqueous sodium bicarbonate followed by acidification and repartitioning with chloroform. After thin layer chromatography and derivatization with dimethylaminobenzaldehyde-HCl spray, the toxin is quantitated by reflection densitometry at 540 nm. The recovery of cyclopiazonic acid averages 90% for peanuts and 85% for corn. The absolute detection limit is 25 ng per spot which translates to a detection limit of 125 micrograms/kg for a 50 g sample.
mesh_terms: D010367:Arachis / Q000032:analysis*; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D007211:Indoles / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D003313:Zea mays / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D007211:Indoles; D009183:Mycotoxins; C000543:cyclopiazonic acid