Effects of oestradiol and progesterone on the metabolism of [U-14C]glucose by mouse morulae and early blastocysts in vitro.
The addition of progesterone (10(-7) to 10(-5) M) and/or oestradiol (10(-10) M) during 24-h chase culture of pulse-labelled morulae-early blastocysts did not affect the degradation of radiolabelled glycogen or other biochemical fractions. The presence of a high concentration of progesterone (10(-5) M) during 5-h pulse culture significantly inhibited incorporation of substrate carbon from [U-14C]glucose into both the acid-soluble and acid-insoluble glycogen fractions, but had no effect on non-glycogen fractions. Catabolic utilization of glucose as estimated by the rate of carbon dioxide and lactate production was not affected by the presence of progesterone (10(-7) to 10(-5) M), oestradiol (10(-10) to 10(-8) M) or a combination of both. The results indicate that ovarian steroids at expected physiological concentrations do not directly influence embryonic energy metabolism.
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D002970:Cleavage Stage, Ovum / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D009028:Morula / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*
D011374:Progesterone; D004958:Estradiol; D005947:Glucose