title: Membrane methylation in isolated rat testis interstitial cells unmasks functional luteinizing hormone receptors.
abstract: Treatment of intact isolated rat testis interstitial cells with S-adenosylmethionine as methyl donor, increases substantially the number of LH human CG receptors (100-200%) without modifying the equilibrium dissociation constant. The increase in binding capacity was associated with an augmentation in the sensitivity of the rat testis interstitial cells to produce testosterone in response to LH, suggesting a functional role of the unmasked receptors. The amount of S-adenosylmethionine necessary to obtain an increase in LH binding capacity and preserve cell viability was 25-50 micrograms/ml per 1.6 X 10(7) cells. 10 mM MgCl2 in addition to the Mg2+ present in the medium was necessary to maintain cell viability. 3H-labelled methyl groups were incorporated mainly into the lipid fraction (208 fmol/10(6) cells) when 3H-S-adenosylmethionine was incubated with the cells for 2 h at 30 degrees C. Our results are consistent with the conclusion that early action of LH may involve an activation of methyltransferase activity, phospholipid methylation, an increase in LH binding capacity and an increase in receptor function.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000378:metabolism; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000502:physiology*; D008297:Male; D008563:Membrane Lipids / Q000502:physiology*; D008745:Methylation; D051381:Rats; D011974:Receptors, LH / Q000502:physiology*; D012436:S-Adenosylmethionine / Q000494:pharmacology; D013737:Testis / Q000502:physiology*; D013739:Testosterone / Q000096:biosynthesis
chemical_list: D006063:Chorionic Gonadotropin; D008563:Membrane Lipids; D011974:Receptors, LH; D013739:Testosterone; D012436:S-Adenosylmethionine; D007986:Luteinizing Hormone