[31P-NMR and HPLC studies on the energy metabolism of perfused chondrocytes].
Energy metabolism of perfused rabbit ear chondrocytes was studied by using 31P-nuclear magnetic resonance (31P-NMR) and high performance liquid chromatography (HPLC). Rabbit ear chondrocytes contained a relatively low concentration of creatine phosphate and high concentrations of GTP and UTP, compared with that of ATP. Concentrations of high-energy phosphate compounds and pH in the chondrocytes did not change when the oxygen concentrations of the perfusate were 95, 20 and 7%. However, high-energy phosphate compounds decreased when the perfusate did not contain oxygen, and when it contained 1.0 mM KCN or 0.1 mM dinitrophenol. A decrease in pH due to accumulation of lactate was observed simultaneously. The findings suggested that a large part of high-energy phosphate compounds in rabbit ear chondrocytes are consumed in the process of matrix production. Oxygen supply was necessary in order to maintain intracellular concentrations of these high-energy phosphate compounds.
D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009705:Nucleosides / Q000378:metabolism; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011817:Rabbits; D013403:Sugar Phosphates / Q000378:metabolism
D009705:Nucleosides; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D013403:Sugar Phosphates; D010725:Phosphocreatine