title: Ultrastructural investigations on the question of mechanical activation of blood platelets.
abstract: The present study addresses the question whether platelets are activated by mechanical stresses as they occur in pathologically accelerated blood flow. Their potential mechanoreceptive properties were tested by subjecting human platelets to defined fluid mechanical forces for periods of milliseconds. Platelet activation was assessed by quantitative morphology, revealing besides activated platelets, irreversibly ballooned, lytic platelets. However, this morphometrically documented "shear activation" of platelets can be suppressed almost completely by the addition of enzyme-substrate systems, capable of removing adenosine diphosphate from the suspending medium. This is in keeping with a recent study from our laboratory showing that the behaviour of lactic dehydrogenase as marker for platelet lysis and beta-thromboglobulin as release marker refuted earlier data, suggesting a direct activation of platelets by shear. It is concluded that former evidence of "shear induced platelet activation" must be interpreted as the consequence of lytic damage to a small proportion of platelets.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D013314:Stress, Mechanical
chemical_list: D000244:Adenosine Diphosphate