Sensitivity to Parietaria officinalis and Parietaria judaica pollen allergens in a Spanish population.
To investigate the biological activity of P. judaica and P. officinalis allergen preparations, 36 Spanish patients were included in a biological standardization (BS) trial. For both species the concentration corresponding to 1000 biological units/ml (BU/ml) was found to be about 2 micrograms dry weight/ml (d.w./ml). The allergenic activity per microgram d.w. was about the same as reported for other important weed pollen extracts. The physico-chemical and immunological properties of the purified Parietaria judaica and Parietaria officinalis pollen allergen preparations used for biological standardization were investigated. The protein content was determined to 38% and 23% for P. judaica and P. officinalis respectively, while the carbohydrate content was 25% in both weed preparations. More than 40 antigens and at least 10 different allergens were detected by crossed immuno electrophoresis/crossed radio immunoelectrophoresis (CIE/CRIE) analyses. SDS-gPAGE followed by electroblotting showed important allergens between 10,000 and 75,000 d for both species. The crossreactivity as measured by skin prick test, RAST and CRIE was high.
D000485:Allergens / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000453:epidemiology / Q000276:immunology*; D007123:Immunoelectrophoresis, Two-Dimensional; D007428:Intradermal Tests; D010940:Plant Proteins / Q000276:immunology; D010944:Plants / Q000276:immunology*; D011058:Pollen / Q000032:analysis / Q000276:immunology*; D011852:Radioallergosorbent Test; D012015:Reference Standards; D013030:Spain; D013045:Species Specificity
D000485:Allergens; D006023:Glycoproteins; D010940:Plant Proteins