Genetic transformation of chickens using irradiated male gametes.
Results have been obtained which corroborate those of Pandey and Patchell (Molec. Gen. Genet., 186, 305, 1982) in demonstrating that genetic material from irradiated semen is incorporated into the embryo and expressed, albeit at rather a low rate, and is subsequently transmitted to progeny of the transfected birds. The method provides a technically straightforward means of transferring genetic material where rapid and reliable means of detecting the transferred gene exist. An advantage of the method is that regulatory regions are likely to be carried with the transferred gene but there is equally a disadvantage in the simultaneous transfer of unwanted material.
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003433:Crosses, Genetic; D005260:Female; D005796:Genes / Q000528:radiation effects*; D006239:Haplotypes; D008285:Major Histocompatibility Complex / Q000528:radiation effects; D008297:Male; D010641:Phenotype; D012661:Semen / Q000528:radiation effects; D013094:Spermatozoa / Q000528:radiation effects*; D014170:Transformation, Genetic