title: Computer-aided hybridoma screening. Production of monoclonal antibodies against membrane fractions from mammary tumour tissue.
abstract: Monoclonal antibodies were raised against membrane fractions from mammary tumour tissue. 746 hybridomas were obtained against the so-called membrane fraction '2a' and 1393 hybridomas against the so-called 'top-region'. These hybridomas were tested with an immunodot assay, cell-binding radioimmunoassay and an immunocytological screening assay using several antigens. In the evaluation of these data we used the common database software (dBASE II) on a personal computer. To facilitate computer handling, even for the non-specialist, we programmed our individual menu-driven software for computer-aided hybridoma screening.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000912:Antibodies, Neoplasm / Q000276:immunology / Q000302:isolation & purification*; D000951:Antigens, Neoplasm / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology*; D003201:Computers; D006825:Hybridomas / Q000032:analysis* / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology*; D008838:Microcomputers; D012984:Software
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm