title: [Composition, structure and various properties of DNA-protein complexes located at the sites of DNA loop attachment to the nuclear skeleton].
abstract: The intimate structure of the complexes located at the sites of DNA loops attachment to the nuclear skeleton was analysed. It is shown that: there are at least three components of the attachment site complex: DNA, protein, RNA; protein moiety consists of 7-8 species with Mr 70-17 kDa. Their association with DNA is resistant to ionic detergents, high salt and urea treatments. The DNA-protein complex is also resistant to the SDS-pronase-phenol deproteinisation procedure; the buoyant density of the complex is the same as DNA density. RNase digestion at low ionic strength reduces density of the complex while the same treatment at 0,4 M NaCl has no effect; DNA-protein complexes isolated with urea-high salt treatment are visualised as globular particles 25-35 nm in diameter with DNA loops attached. These particles were not observed after detergent treatment although protein composition of the complex remained the same.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis* / Q000648:ultrastructure; D046911:Macromolecular Substances; D051379:Mice; D009698:Nucleoproteins / Q000032:analysis*; D010954:Plasmacytoma
chemical_list: D046911:Macromolecular Substances; D009698:Nucleoproteins; D004247:DNA
vernacular_title: Sostav, stroenie i nekotorye svoĭstva kompleksov DNK s belkom, raspolozhennykh v uchastkakh prikrepleniia petel' DNK k iadernomu skeletu.