A rapid and simple method of extracting asbestos bodies from lung tissue by cytocentrifugation.
During analyses of alkali digested lung tissue for asbestos bodies, we observed that the number of asbestos bodies in the discarded waste frequently exceeded the number in the filtered residue, the number reported in the standard diagnostic method. This observation led to the exploration of alternative techniques that would optimize the recovery of asbestos bodies. We describe a new, simple, and rapid method for extracting asbestos bodies from digested lung tissue using a cytocentrifuge, in which the waste extraction and filtration steps are eliminated. Samples of digested lung tissue are ready for light microscopy after 10 minutes of cytocentrifugation directly onto a glass slide. The standard method was compared with the cytocentrifuge technique using lung tissue from four asbestos-exposed workers and four controls with no known history of exposure. The number of asbestos bodies extracted by the centrifuge method was, on average, seven times higher than the number found with the standard method. A detailed quantitative study was made of the case that had the most asbestos bodies (comparison of the number of asbestos bodies counted in both "residue" and "waste", applying the filtration and cytocentrifuge methods). The number of asbestos bodies found in the discarded waste significantly exceeded the number in the "reportable" filtered residue.
D000328:Adult; D001194:Asbestos / Q000302:isolation & purification*; D002498:Centrifugation; D002725:Chloroform; D000431:Ethanol; D006801:Humans; D008168:Lung / Q000032:analysis*; D008722:Methods
D001194:Asbestos; D000431:Ethanol; D002725:Chloroform