Histone H2B repression causes cell-cycle-specific arrest in yeast: effects on chromosomal segregation, replication, and transcription.
To determine which cellular processes are dependent on histones, we blocked histone H2B mRNA synthesis in asynchronously growing yeast after fusing the H2B gene to a repressible GAL10 promoter. Chromosomal segregation, replication, and transcription were then examined. We found that the cells arrested in mitosis, with a cell division cycle (cdc) phenotype. Chromatin structure and nuclear segregation were disrupted. A full round of DNA replication took place after the repression of histone H2B mRNA synthesis. Active transcription and the induction of new transcripts also continued in the arrested cells.
D002453:Cell Cycle / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D004271:DNA, Fungal / Q000096:biosynthesis; D006657:Histones / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D015003:Yeasts / Q000166:cytology / Q000235:genetics*
D004271:DNA, Fungal; D006657:Histones; D012331:RNA, Fungal; D012333:RNA, Messenger