Production of monoclonal antibodies to salinomycin.
Protein conjugates of salinomycin were prepared and utilized to produce murine monoclonal antibodies (MAbs) in the BALB/c-P3X63.Ag 8.653 fusion system. Several antibodies were cloned, stabilized, and evaluated for cross-reactivity to other ionophorous antibiotics. Selected antibodies were used to develop quantitative assays for salinomycin by means of an enzyme-linked immunosorbent assay (ELISA). These assays were sensitive to 50 ng of salinomycin.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000276:immunology; D011714:Pyrans / Q000032:analysis / Q000276:immunology; D000095662:Polyether Polyketides
D000911:Antibodies, Monoclonal; D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides