Production of monoclonal antibodies. The effect of hybridoma concentration on the yield of antibody-producing clones.
The relationship between the number of hybrid clones in each well of the primary culture, the yield of antibody-producing wells and the short-term stability of the polyclonal cultures was tested. The results of the study demonstrate that a higher yield of antibody-secreting clones was obtained by employing lower density of hybrids and that instability of antibody secretion by polyclonal cultures is not the result of overgrowth of non-secreting clones.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology; D046508:Culture Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal