Fourier transform infrared spectra of aqueous protein mixtures using a novel attenuated total internal reflectance cell with infrared fibers.
A specially designed cell containing a silver halide (AgBr:AgCl) infrared fiber allows convenient and reproducible loading of viscous protein solutions and suspensions. Attenuated total internal reflectance measurements using an FTIR spectrometer were made for bovine serum albumin-water past. Dynamic changes in the protein films are readily followed, a technique which should be generally useful. A band assigned to a secondary structural feature, the alpha-helix, is similar in intensity to that reported (T.M. Fong, M.G. McNamee, submitted for publication).
D001965:Bromides; D005583:Fourier Analysis; D011487:Protein Conformation; D012710:Serum Albumin, Bovine; D012834:Silver; D018030:Silver Compounds; D012996:Solutions; D013055:Spectrophotometry, Infrared / Q000295:instrumentation; D014867:Water
D001965:Bromides; D018030:Silver Compounds; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; D012834:Silver; C037548:silver chloride; C047751:silver bromide