Improved enzymatic hydrolysis of conjugated bile acids.
The hydrolysis of conjugated bile acids by cholylglycine hydrolase (EC 3.5.1.24) using the standard procedure for a commercial enzyme preparation was found to be incomplete, as judged by the use of 24-14C-taurocholic acid as a tracer. A method is proposed that incorporates the nonionic detergent Triton X-100 into the reaction mixture to achieve almost complete hydrolysis. It is proposed that the observed enhancement of enzyme activity is due to the formation of micelles by the detergent.
D000581:Amidohydrolases / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000097:blood*; D006801:Humans; D008823:Micelles; D017830:Octoxynol; D011092:Polyethylene Glycols; D013654:Taurine / Q000378:metabolism; D013656:Taurocholic Acid / Q000378:metabolism; D014580:Ursodeoxycholic Acid / Q000378:metabolism
D001647:Bile Acids and Salts; D008823:Micelles; C030141:taurine-ursodeoxycholate conjugate; D013654:Taurine; D011092:Polyethylene Glycols; D013656:Taurocholic Acid; D014580:Ursodeoxycholic Acid; D017830:Octoxynol; D000581:Amidohydrolases; C023154:choloylglycine hydrolase