[Comparative study of ceruloplasmin biosynthesis in various cell-free systems].
Some peculiarities of mRNA translation of ceruloplasmin (CP) from rat liver were investigated, using three cell-free protein biosynthesis systems (wheat embryo extracts, rabbit reticulocyte lysates and Zajdela ascite hepatoma extracts). It was shown that reticulocyte lysates and tumour cell extracts synthesize full-size CP mRNA translation products, whose molecular mass is close to that of mature CP molecules, i. e., 122-132 kD. Wheat embryo extracts synthesize the NH2-terminal fragment of the CP molecule (Mr = 84 kD). Addition of liver membrane fractions to wheat embryo extracts translating CP mRNA results in the reconstitution of proteolytic steps of CP maturation.
D000818:Animals; D002474:Cell-Free System; D002570:Ceruloplasmin / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism
D011480:Protease Inhibitors; D002570:Ceruloplasmin
Sravnitel'noe izuchenie biosinteza tseruloplazmina v razlichnykh beskletochnykh sistemakh.