Rapid purification of the main allergen of Lolium perenne by high-performance liquid chromatography.
The main allergen from rye grass (Lolium perenne) pollen was purified by size-exclusion high-performance liquid chromatography. The purified allergen had a molecular weight of 32,000 daltons and was significantly more active in solid phase radioimmunoassay than the whole extract. The highly purified antigen can be obtained very rapidly and with a recovery of 30%.
D000485:Allergens / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D008129:Lolium / Q000032:analysis*; D006109:Poaceae / Q000032:analysis*; D011058:Pollen / Q000032:analysis; D011480:Protease Inhibitors; D011863:Radioimmunoassay
D000485:Allergens; D011480:Protease Inhibitors