Serum antibodies to LDH-C4.
Human sera from the WHO Reference Bank were tested by a liquid phase radioimmunoassay for antibodies to the sperm-specific form of lactate dehydrogenase (LDH-C4; LDH-X). Modestly elevated levels of antibodies were detected in about 10% of infertile males and females and in about 20% of vasectomized males, as well as in a few cases in other categories. The occurrence of this anti-LDH-C4 activity was without overt correlation with other anti-sperm antibody assays. The primary advantage of the radioimmunometric assay is its absolute specificity for a sperm antigen. These findings lend support to contraceptive vaccine development based on LDH-C4 antigenicity.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000276:immunology*; D007248:Infertility, Male / Q000097:blood / Q000276:immunology*; D007457:Iodine Radioisotopes; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D008297:Male; D051379:Mice / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D013094:Spermatozoa / Q000201:enzymology / Q000276:immunology; D014659:Vasectomy; D014944:World Health Organization
D000906:Antibodies; D007457:Iodine Radioisotopes; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase