Preparation of agarose with low net negative charge density using an inexpensive anion exchanger.
A method of preparing agarose of low net negative charge density by the use of Chromium tanned gelatin is described. Using commercial agarose (agarose c) as standard of comparison the product has a sulphate content approximately 0.1 that of agarose c and its pyruvate content was reduced to zero as shown by NMR in DMSO. The product was still able to adsorb and desorb serum glycoproteins similar to agarose c indicating that charge on the agarose c gel particles played a minor role if any on the binding of the serum glycoproteins.
D000327:Adsorption; D000837:Anion Exchange Resins; D002236:Carbohydrate Conformation; D002857:Chromium; D004563:Electrochemistry; D005780:Gelatin; D006023:Glycoproteins / Q000097:blood*; D007475:Ion Exchange Resins; D011773:Pyruvates; D019289:Pyruvic Acid; D012685:Sepharose / Q000032:analysis; D013431:Sulfates / Q000032:analysis
D000837:Anion Exchange Resins; D006023:Glycoproteins; D007475:Ion Exchange Resins; D011773:Pyruvates; D013431:Sulfates; D002857:Chromium; D019289:Pyruvic Acid; D005780:Gelatin; D012685:Sepharose