Solid phase synthesis of thymosin beta 9.
Thymosin beta 9, a 41 residue thymic polypeptide, has been synthesized by a solid phase method. A modification of the low HF method was used to deprotect and cleave the peptide from the resin. Thymosin beta 9 was then obtained in analytically pure form by a one-step purification procedure in 32% yield. The activity of thymosin beta 9 in the terminal deoxynucleotidyl transferase assay was greater than calf thymus fraction 5, but comparable to thymosin beta 4. In contrast to thymosin alpha 1, neither beta 4 nor beta 9 was active in the rosette inhibition assay.
D000818:Animals; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D007202:Indicators and Reagents; D051379:Mice; D008810:Mice, Inbred C57BL; D013329:Structure-Activity Relationship; D013947:Thymosin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D013950:Thymus Gland / Q000201:enzymology
D007202:Indicators and Reagents; D013947:Thymosin; C042362:thymosin beta(9); D004253:DNA Nucleotidylexotransferase; D006854:Hydrocortisone