title: A set of procedures for resolving purine compounds by reversed-phase high performance liquid chromatography: application to the study of purine nucleotide and nucleic acid metabolism.
abstract: A set of simple procedures for the separation of major purine 5'-ribonucleotides including diguanosine polyphosphates, purine and pyrimidine bases, and 2'- and 3'-nucleotide monophosphates using reversed-phase high-performance liquid chromatography and isocratic elution study of purine nucleotide and nucleic acid biosynthesis in Artemia is presented.
mesh_terms: D000818:Animals; D001156:Artemia / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D009696:Nucleic Acids / Q000032:analysis* / Q000096:biosynthesis; D011685:Purine Nucleotides / Q000096:biosynthesis / Q000302:isolation & purification*; D011687:Purines / Q000302:isolation & purification; D011743:Pyrimidines / Q000302:isolation & purification; D012265:Ribonucleotides / Q000302:isolation & purification; D012995:Solubility
chemical_list: D009696:Nucleic Acids; D011685:Purine Nucleotides; D011687:Purines; D011743:Pyrimidines; D012265:Ribonucleotides