[Effect of physical exercise on the level of the non-activated form of glucocorticoid receptor in cytosol and phosphorylation of non-histone proteins of the myocardium].
In accordance with recently published data on the nuclear localization of free steroid receptors in vivo and the role of phosphorylation processes in the hormonal control of cellular activity, an attempt was made to study the effect of physical exercise on the phosphorylation of cytosolic and nuclear proteins and on the proportion of nonactivated and activated forms of the glucocorticoid receptor in the hearts of adrenalectomized rats. According to a generally accepted hypothesis, the receptor in these animals resided mainly in the cytoplasm. The results of DNA- and DEAE-cellulose chromatography showed that physical exercise caused an increase in the amount of the nonactivated receptor. As the amount of the activated form remained approximately the same the best explanation was that some amount of the receptor resided in the nuclei even in adrenalectomized rats and physical exercise decreased the affinity of the receptor to nuclear elements. At the end of exhaustive exercise the in vivo phosphorylation of nuclear nonhistone proteins was decreased, however dexamethasone administration enhanced phosphorylation and also caused the receptor translocation into nuclei. The results obtained suggest that both the binding of the hormone to its receptor (leading to closer association with nuclear elements, probably with nonhistone proteins) and the phosphorylation of nonhistones are biologically related events involved in the regulation of gene transcription.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010766:Phosphorylation; D005082:Physical Exertion; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D011965:Receptors, Glucocorticoid
Vliianie fizicheskikh nagruzok na soderzhanie neaktivirovannoĭ formy gliukokortikoidnogo retseptora v tsitozole i fosforilirovanie negistonovykh belkov miokarda.