[Polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism].
A procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. The procedure involved polarographic registration of oxygen consumption, which is directly related to content of NADPH formed, in a coupled enzymatic system containing peroxidase and Mn2+.
D004912:Erythrocytes / Q000201:enzymology* / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006801:Humans; D010427:Pentose Phosphate Pathway; D010734:Phosphogluconate Dehydrogenase / Q000097:blood*; D011048:Polarography
D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase
Poliarograficheskiĭ metod opredeleniia aktivnosti degidrogenaz pentozofosfatnogo puti metabolizma uglevodov.