title: Changes in H1 complement in differentiating rat-brain cortical neurons.
abstract: Neuronal nuclei have a low H1 content. A stoichiometry of 0.47 molecule/nucleosome, on average, is calculated for rat brain cortical neurons by comparing its H1 content with that of liver nuclei. The H1 fraction of rat cerebral cortex neurons has been resolved into five subtypes, H1a--e, that have the same mobility as the unphosphorylated H1 forms of other rat tissues. The subtypes H1a--d decay exponentially during postnatal development and are substituted to different extents by H1e. The higher replacement rate is shown by H1a with an apparent half-lifetime of about 5 days. The corresponding values for H1b, H1c and H1d are 11, 21 and 15 days. Several conclusions can be drawn from the observation of postnatal changes in H1 subtype proportions. The low H1 content of neuronal nuclei does not imply the presence of notable peculiarities in subtype composition or in subtype substitution pattern. There is turnover of H1 in differentiating neurons once cell proliferation and DNA replication have ceased. The relative rates of synthesis and/or degradation of the subtypes differ in germinal cells and in neurons. Comparison with previous results on H1 degrees accumulation also shows that in cortical neurons the regulation of the subtypes H1a--e differs from that of H1 degrees.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002467:Cell Nucleus / Q000032:analysis; D002540:Cerebral Cortex / Q000166:cytology* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D006207:Half-Life; D006657:Histones / Q000032:analysis*; D008099:Liver / Q000166:cytology; D008433:Mathematics; D009474:Neurons / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006657:Histones