title: Use of cells cultured from human mammary carcinomas for studies of malignant progression and chemotherapeutic drug sensitivity.
abstract: We describe techniques to readily culture human mammary epithelium from normal and malignant tissue. The cells were characterized as to their epithelial origins and qualities distinguishing tumor from normal cells. Using a highly efficient clonogenic assay, we now are able to routinely assay breast tumors for chemotherapeutic drugs. Initial studies suggest that these assays correlate with patient response.
mesh_terms: D001940:Breast / Q000166:cytology; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology*; D002277:Carcinoma / Q000188:drug therapy / Q000473:pathology*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004317:Doxorubicin / Q000494:pharmacology; D005260:Female; D006801:Humans; D009362:Neoplasm Metastasis; D014410:Tumor Stem Cell Assay
chemical_list: D004317:Doxorubicin