title: Chlamydial infection of subcutaneous fimbrial transplants in cynomolgus and rhesus monkeys.
abstract: Acute infection with Chlamydia trachomatis serotype E was established in monkey fallopian tube fimbriae by subcutaneous implantation. Depending upon monkey species, from eight to 20 implants could be established in each animal. Animals were given estrogen before percutaneous inoculation of the autografts with Chlamydia. Acute inflammatory changes were found in homografts examined in the first week after infection, with chronic inflammatory changes noted thereafter. Chlamydial inclusions were detected within fimbrial epithelial cells up to seven days postinoculation by fluorescent-antibody staining and immunoperoxidase staining with C. trachomatis-specific monoclonal antibody. Organisms were recovered from autografts up to five days after infection. Analysis of serum antibody by microimmunofluorescence revealed that serotype E-specific antibody of both IgM and IgG classes was produced after infection. We conclude that subcutaneously implanted fallopian tube autografts may provide a useful primate model for kinetic studies of chlamydial infection and immunity.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D002692:Chlamydia trachomatis / Q000276:immunology / Q000302:isolation & purification; D004195:Disease Models, Animal; D005187:Fallopian Tubes / Q000382:microbiology / Q000473:pathology / Q000637:transplantation; D005260:Female; D008252:Macaca fascicularis; D008253:Macaca mulatta; D012488:Salpingitis / Q000276:immunology / Q000382:microbiology / Q000473:pathology*
chemical_list: D000907:Antibodies, Bacterial