Physical properties of chondroitin sulphate/dermatan sulphate proteoglycans from bovine aorta.
Bovine aortic chondroitin sulphate/dermatan sulphate proteoglycans (PG-25, PG-35 and PG-50) were differentially precipitated with ethanol and analysed by a variety of chemical and physical techniques. The glycosaminoglycan chains of PG-25 and PG-35 contained a mixture of glucuronic acid and iduronic acid, whereas the uronic acid component of PG-50 was primarily glucuronic acid. In addition, various amounts of oligosaccharides containing small amounts of mannose, a galactose/hexosamine ratio of 1:1 and an absence of uronic acid were covalently linked to the core protein of all proteoglycans. The weight-average Mr (Mw) values of the proteoglycans determined by light-scattering in 4 M-guanidinium chloride were 1.3 X 10(6) (PG-25), 0.30 X 10(6) (PG-35) and 0.88 X 10(6) (PG-50). The s0 values of the proteoglycans were distributed between 7 and 8 S, and the reduced viscosities, eta sp./c, of all proteoglycans were dependent on the shear rate and polymer concentration. Electron microscopy of spread molecules revealed that PG-25 contained small structural units that appeared to self-associate into large aggregates, whereas PG-35 and PG-50 appeared mainly as monomers consisting of a core with various numbers of side projections. Hyaluronic acid-proteoglycan complexes occurred only with a small proportion of the molecules present in PG-35, and their formation could be inhibited by oligosaccharides. These results suggest the presence in the aorta of subspecies of chondroitin sulphate and dermatan sulphate proteoglycans, which show large variations in their physicochemical and inter- and intra-molecular association properties.
D000818:Animals; D001011:Aorta / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002807:Chondroitin / Q000031:analogs & derivatives; D011508:Chondroitin Sulfate Proteoglycans; D002849:Chromatography, Gas; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D008027:Light; D008854:Microscopy, Electron; D011509:Proteoglycans; D012542:Scattering, Radiation; D014461:Ultracentrifugation; D014574:Uronic Acids / Q000032:analysis; D014783:Viscosity
D002241:Carbohydrates; D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D011509:Proteoglycans; D014574:Uronic Acids; C032649:dermatan sulfate proteoglycan; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002807:Chondroitin