[Cell-killing effect in vitro of prostaglandin derivatives on an established human bladder cancer cell line, KK-47].
The cytotoxic effects of the prostaglandin derivatives, NP01A, NP07A and NP13A (Nihoniyakuhin Kogyo Co., Ltd., Toyama, Japan) on asynchronous cells of an established human bladder cancer cells line, KK-47, have been investigated using an in vitro colony formation technique. The following results obtained: NP01A-and NP13A-treated cells presented survival curves showing an exponential decrease with increasing drug concentrations. NP07A-treated cells demonstrated a survival curve bearing a prominent shoulder at considerably high drug concentrations. Using these survival curves, the 50% and 90% cell growth inhibition concentrations (IC50 and IC90) of the derivatives were computed. NP13A showed the highest cell-killing effect in terms of IC50 or IC90. According to a statistical analysis of variances followed by unpaired t-test, NP07A showed a higher cell-killing effect than that of NP01A (P less than 0.01). Based on a therapeutic index obtained from the ratio of clinical dosage to IC90, the derivatives proved to be of value in further in vivo studies.
D000527:Alprostadil / Q000031:analogs & derivatives*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004351:Drug Resistance; D006801:Humans; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D011459:Prostaglandins E, Synthetic; C046219:NP 13 A; C046217:NP 01A; C046218:NP 07 A; D000527:Alprostadil