Glutathione content in the semen of bulls of the lowland black-white breed.
Glutathione concentration in the semen was determined in 113 ejaculates obtained from 30 bulls of the lowland black-white breed. The quantitative and morphological characteristics of the studied sperm samples indicated considerable individual differences but always within normal range. Glutathione content was determined in the plasma and in isolated spermatozoa by the method of Saville (Analyst, 1958, 83, 670). A high glutathione level in the spermatozoa (31 nmols in 10(9) cells) and in plasma (28.8 microM) was found. A positive correlation was demonstrated (p less than 0.05) between the level of the peptide in plasma and its level in spermatozoa. It was found that the glutathione level in spermatozoa was higher in the ejaculates with the cell count below the average level.
D000177:Acrosome / Q000648:ultrastructure; D000818:Animals; D002417:Cattle; D005978:Glutathione / Q000032:analysis*; D008297:Male; D012661:Semen / Q000032:analysis*; D013045:Species Specificity; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000032:analysis*
D005978:Glutathione