Proliferative characteristics and sister chromatid exchange (SCE) of colony-forming cells (CFU-S) in acute myelogenous leukemia.
Sister chromatid exchange (SCE) and cell cycle progression of colony-forming cells (CFU-S) from patients with acute myelogenous leukemia were studied using bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. The mean SCE rate of CFU-S was 7.99, which is similar to that reported for human peripheral blood lymphocytes. Cultures treated with BrdU at culture initiation and then harvested 72-120 hr later indicated a cell cycle time (TC) of approximately 60 hr. However, cells allowed to proliferate for 72-120 hr prior to the addition of BrdU showed TC values of 12-36 hr. Analysis of serial cultures from one patient revealed a heterogeneous population with a mean TC of 19.5 hr. These cell cycle times are considerably shorter than previously published reports and suggest that leukemic cells have the potential to divide rapidly.
D000328:Adult; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology* / Q000648:ultrastructure; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013234:Stem Cells / Q000473:pathology*; D013997:Time Factors
D001973:Bromodeoxyuridine