Effects of microwave radiation (340 and 900 MHz) on different structural levels of erythrocyte membranes.
By use of fluorescence probes 1-anilinonaphthalene-8-sulfonic acid, 2-toluidinylnaphthalene-6-sulfonate, pyrene, perylene and chemical label phosphatidylethanolamine 2,4,6-trinitrobenzele sulfonic acid, the effect of microwave radiation on the erythrocyte membrane was studied. The studies with the fluorescence probes were carried out on erythrocyte ghosts and with 2,4,6-trinitrobenzene sulfonic acid on whole erythrocytes. The fluorescence was measured during irradiation of the membranes with 340-MHz microwaves at an SAR of 100 W/kg. Trinitrophenylation of phosphatidylethanolamine from whole erythrocytes was performed simultaneously with microwave irradiation at 900 MHz (10 mW/cm2). It was shown that the microwave field decreased lipid viscosity, altered the structural state of lipid-protein contact regions, and decreased the protein shielding of lipids. These changes corresponded to those produced by thermal action of microwaves.
D000818:Animals; D001798:Blood Proteins / Q000528:radiation effects; D004910:Erythrocyte Membrane / Q000528:radiation effects*; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000528:radiation effects; D008565:Membrane Proteins / Q000528:radiation effects; D008872:Microwaves; D051381:Rats
D001798:Blood Proteins; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D008565:Membrane Proteins