The effects of zinc chloride on the RNP structures in HEp-2 cells: accumulation of perichromatin granules.
The effects of zinc on the ribonucleoprotein (RNP) constituents of HEp-2 cells have been analyzed. Pulse-chase autoradiographic experiments show a preferential inhibition of nucleolar RNA synthesis and a block in the transport of nucleolar and extranucleolar RNA in zinc-treated cells. Concomitantly with the disturbance in RNA metabolism and in protein synthesis, nucleolar condensation, accumulation of perichromatin granules and fibrils, condensation of interchromatin fibrils, and appearance of dense granular bodies occur. Accumulation of perichromatin fibrils and condensation of interchromatin fibrils appear to be related to the block in the transport of heterogeneous nuclear RNA. Depletion of certain proteins required for the assembly of RNP particles could share in the abnormal behavior of RNA and lead to the accumulation of perichromatin granules and the appearance of dense granular bodies.
D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002712:Chlorides; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D007700:Kinetics; D008854:Microscopy, Electron; D012261:Ribonucleoproteins / Q000378:metabolism*; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds
D002712:Chlorides; D002843:Chromatin; D012261:Ribonucleoproteins; D017967:Zinc Compounds; D014316:Tritium; C016837:zinc chloride; D015032:Zinc; D014529:Uridine