Determination of 5-aminolevulinic acid in biological samples by high-performance liquid chromatography.
5-Aminolevulinic acid (ALA), the common precursor of all naturally occurring tetrapyrroles, forms a stable condensation product with 2-amino-3-hydroxynaphthalene which can be identified by its fluorescence. Separation of the compound by reversed-phase high-performance liquid chromatography on RPC-18 columns allows its detection down to the picomolar range and can be successfully applied for ALA analysis in small biological samples. The reaction product of ALA with 2-amino-3-hydroxynaphthalene has been synthesized and characterized.
D000622:Aminolevulinic Acid / Q000032:analysis*; D002708:Chlorella / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D007982:Levulinic Acids / Q000032:analysis*; D008832:Microchemistry; D009284:Naphthols; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D007982:Levulinic Acids; D009284:Naphthols; C049196:2-amino-3-hydroxynaphthalene; D000622:Aminolevulinic Acid