Characterization of rat ribosomal DNA II. identification of the highly repetitive DNA in the 3' non-transcribed spacer.
The nucleotide sequence of one of the non-transcribed spacer subclones, p1.7, from the region 3' to rat 45 S pre-rRNA has been determined. Within 1612 base-pairs, the fragment contains two distinct regions of highly repetitive DNA, one of which can serve as a site for initiation in vitro by RNA polymerase III. The first is the alternating purine-pyrimidine sequence (A-C)21. The second of these regions has 95% homology to the identifier sequence and served as the template for RNA polymerase III transcription in vitro. The in vitro polymerase III template is aligned in opposite polarity to the direction of transcription of 45 S rRNA. Located near the identifier sequence is a region that is 59% homologous to the type-II Alu sequences. It would seem, therefore, that members of more than one highly repetitive sequence family have accumulated in the non-transcribed spacers. These data also suggest that within the non-transcribed spacers these families have evolved (sequence variation) at different rates, until one of them, the Alu type-II-like element, may represent a new Alu type-II subfamily.
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D004274:DNA, Recombinant; D004275:DNA, Ribosomal / Q000235:genetics*; D005095:Exoribonucleases; D009841:Oligonucleotides; D012335:RNA, Ribosomal; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D009841:Oligonucleotides; D012335:RNA, Ribosomal; D005095:Exoribonucleases; C042081:exoribonuclease T