Expression of coagulant activity in human platelets: release of membranous vesicles providing platelet factor 1 and platelet factor 3.
The relationship between the appearance of membrane-associated factor V-like activity (platelet factor 1, PF1) and phospholipid-like catalytic activity (platelet factor 3, PF3) has been examined, in vitro, in collagen-stimulated, human platelets. Both activities increased 7 fold upon collagen treatment relative to stirred controls. After sedimentation of stimulated platelets, 31% of total PF1 and 41% of PF3 remained in the supernatant fraction. PF1 eluted from a Sepharose CL-4B column in the same void volume fractions as PF3, phospholipid, and vesicular particles. These fractions had roughly 100 fold (lipid basis) or 1000 fold (protein basis) enhanced specific activity when compared to the stimulated platelet suspension. Freeze-fracture electron microscopy demonstrated that these void volume fractions contain two populations of membranous vesicles (80-200 nm and 400-600 nm in diameter). Upon centrifugation of the void volume fractions, PF1 and PF3 activities, phosphate-containing material, and ultraviolet-absorbing material all sedimented at the same rate, indicating that PF1 and PF3 are activities associated with one or both of the platelet-derived vesicle populations. Finally, we examined the effects of inhibitors on the appearance of PF1, PF3, platelet factor 4, total intrinsic factor V activity, and serotonin as well as on platelet aggregation. These studies suggest that the collagen-stimulated release of PF1 and PF3 is not coupled to either platelet aggregation or PF4 release but is probably a separate phase of the release reaction.
D001779:Blood Coagulation Factors / Q000032:analysis / Q000502:physiology*; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D002850:Chromatography, Gel; D003094:Collagen / Q000494:pharmacology; D005165:Factor V / Q000502:physiology; D005166:Factor V Deficiency / Q000097:blood / Q000503:physiopathology; D006801:Humans; D066298:In Vitro Techniques; D010977:Platelet Factor 3 / Q000032:analysis / Q000502:physiology*
D001779:Blood Coagulation Factors; D010977:Platelet Factor 3; C031546:platelet factor 1; D005165:Factor V; D003094:Collagen