A somatic cell mutant with a kinetically altered 3-hydroxy-3-methylglutaryl coenzyme A reductase.
A somatic cell mutant has been isolated which is resistant to killing and growth inhibition by mevinolin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase. The resistance phenotype is dominant with respect to the wild-type cell and can largely be ascribed to a 6-7-fold lowering of the KM for HMG-CoA. We thus conclude that mevinolin resistance can be utilized to obtain a genetic marker for the structural gene encoding HMH-CoA reductase.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics* / Q000378:metabolism; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007700:Kinetics; D008148:Lovastatin; D009154:Mutation; D009281:Naphthalenes / Q000494:pharmacology; D010053:Ovary
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D009281:Naphthalenes; D008148:Lovastatin; D006903:Hydroxymethylglutaryl CoA Reductases