Reduced cofactor function of human high molecular weight kininogen induced by rat plasma kallikrein.
Plasma kallikrein purified from acetone-activated, plasminogen-free rat plasma yielded in polyacrylamide gel electrophoresis protein bands corresponding to Mr values of 143,000 (main band) and 135,000 (lighter band). After SDS treatment without reduction the protein pattern had changed to two strong bands corresponding to Mr values of 87,000 and 78,000. Gel electrophoresis of kallikrein purified from plasma of rats pretreated with clinical dextran (200 mg/kg intravenously) produced main bands corresponding to Mr values of 120,000-130,000 and 78,000-80,000 for native samples and SDS-treated samples respectively (Johansen & Briseid 1983). Both kinds of kallikrein reduced the capacity of human high molecular weight kininogen to function as a cofactor in the surface-mediated activation of factor XII in a crude plasma preparation. The preparation obtained from plasma of dextran-treated rats was significantly more potent than was the normal kallikrein preparation, both as regards the effect against HMrK, and as an activator of plasminogen.
D015119:Aminocaproic Acid / Q000494:pharmacology; D000818:Animals; D006801:Humans; D007610:Kallikreins / Q000097:blood / Q000494:pharmacology*; D007704:Kininogens / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D007704:Kininogens; D007610:Kallikreins; D010960:Plasminogen Activators; D015119:Aminocaproic Acid