Failure to detect specific binding sites for prostaglandin F-2 alpha in membrane preparations from rat endometrium.
Membrane preparations from endometria of rats in different physiological states (e.g. pseudopregnancy, ovariectomized animals receiving progesterone + oestradiol or oestradiol alone) were studied for [3H]PGF-2 alpha binding by methods which detected PGF-2 alpha binding in ovary preparations and PGE binding in the same endometrial preparations. There was no evidence of high-affinity binding sites for [3H]PGF-2 alpha. Saturable [3H]PGF-2 alpha binding that increased with the onset of uterine sensitivity was detected but this binding does not fulfil all the criteria required for a PGF-2 alpha receptor and is probably due to binding to PG metabolizing enzymes in our preparations, or to binding of [3H]PGF-2 alpha to PGE binding sites. The failure to detect specific PGF-2 alpha binding sites seems to reflect a true absence of these sites in the rat endometrium.
D000818:Animals; D001665:Binding Sites; D002369:Castration; D002462:Cell Membrane / Q000378:metabolism; D015237:Dinoprost; D004717:Endometrium / Q000648:ultrastructure*; D004971:Estrus; D005260:Female; D011247:Pregnancy; D011460:Prostaglandins F / Q000378:metabolism*; D011555:Pseudopregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D011460:Prostaglandins F; D015237:Dinoprost