Cloning and nucleotide sequence determination of the Clostridium pasteurianum ferredoxin gene.
We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain greater than 6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobase-pair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.
D001483:Base Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics*; D003062:Codon / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005288:Ferredoxins / Q000235:genetics*; D005798:Genes, Bacterial; D009876:Operon; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D003062:Codon; D004269:DNA, Bacterial; D005288:Ferredoxins