Induction of a novel nuclear protein (p54) by phorbol esters in mouse erythroleukemia (Friend) cells.
Tumor-promoting phorbol esters including 12-O-tetradecanoylphorbol-13-acetate (TPA), specific inhibitors for erythroid differentiation in mouse Friend cells, induce a newly identified nuclear protein with a molecular weight of 54,000 (p54) in Friend cells. Phorbol, an analogue of TPA with neither tumor-promoting nor differentiation-inhibitory activity, did not induce p54. In a variant cell line of Friend cells which exhibits resistance to TPA in erythroid differentiation, p54 was not induced by TPA. The induction of p54 by TPA was counteracted by erythroid-inducing agents including dimethyl sulfoxide, hexamethylenebisacetamide, and actinomycin D. Throughout these experiments, we have observed an inverse relationship between p54 induction and cellular potential for erythroid differentiation.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004351:Drug Resistance; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D009698:Nucleoproteins / Q000096:biosynthesis*; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D009698:Nucleoproteins; D010704:Phorbols; D008715:Methionine; D013755:Tetradecanoylphorbol Acetate