Intracellular localization of 12-O-3-N-dansylamino TPA in C3H/10T1/2 mouse cell line.
The fluorescent 12-O-tetradecanoylphorbol-13-acetate (TPA) analogue dansyl-TPA allowed the distribution of fluorescent molecules to be visualized in living mouse fibroblasts. The entry of dansyl-TPA into cells occurred within millisecond time scale and was found largely energy independent. Dansyl-TPA was shown to stain the endoplasmic reticulum, Golgi apparatus, mitochondria and nuclear membrane. In addition, dansyl-TPA fluorescence was found to parallel that obtained with rhodamine-conjugated anti-alpha tubulin. The intracellular location of the dansyl-TPA fluorescence can be taken into account to explain the pleiotropic action of TPA in the cells.
D000818:Animals; D002460:Cell Line; D003619:Dansyl Compounds / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D005453:Fluorescence; D051379:Mice; D008809:Mice, Inbred C3H; D008856:Microscopy, Fluorescence; D010704:Phorbols / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000378:metabolism*; D014316:Tritium
D003619:Dansyl Compounds; D010704:Phorbols; D014316:Tritium; C043670:O-(N-dansylamino-3-tetradecanoyl)-12,O-acetyl-13-phorbol; D013755:Tetradecanoylphorbol Acetate