A single step procedure for purification of estradiol 17 beta-dehydrogenase from human placenta.
A new and simple procedure for purification to homogeneity of the soluble estradiol 17 beta-dehydrogenase [E C.1.1.1.62] from human placenta has been developed. The purification is achieved in a single step by affinity-absorption of Cibachrome Blue F3G-A coupled to Sepharose and selective elution with NADP+. Homogeneous estradiol 17 beta-dehydrogenase has a specific activity of 7.2 units/mg. and has been purified 2400-fold with a 93% recovery.
D015067:17-Hydroxysteroid Dehydrogenases / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004960:Estradiol Dehydrogenases / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007700:Kinetics; D009249:NADP; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D014227:Triazines
D014227:Triazines; D009249:NADP; C012842:Cibacron Blue F 3GA; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases