Conformational change of 50 S ribosomes on enzymatic binding of phenylalanyl-tRNA.
Tight couple 70 S ribosomes are converted to loose couple ones on enzymatic binding of phenylalanyl-tRNA. Enzymatic binding at 0 degree C as well as nonenzymatic binding does not lead to any change. Further, no change takes place when the P site is occupied by N-acetylphenylalanyl-tRNA. Loose couple 70 S ribosomes are not affected by either enzymatic or nonenzymatic binding of phenylalanyl-tRNA.
D006160:Guanosine Triphosphate / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D010445:Peptide Elongation Factors / Q000494:pharmacology; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D010445:Peptide Elongation Factors; D012346:RNA, Transfer, Amino Acyl; C036506:elongation factor T; C006383:tRNA, N-acetylphenylalanine-; D006160:Guanosine Triphosphate; D008274:Magnesium