Immunisation of cattle with a recombinant togavirus-vaccinia virus strain.
Genetic engineering techniques have been used to construct a vaccinia virus recombinant which contains and expresses togavirus (Sindbis) genetic information. Intradermal inoculation of this recombinant strain into calves caused a transient pock-type lesion at the site of inoculation and elicited the production of substantial levels of anti-Sindbis virus neutralising antibodies. These results suggest that recombinant vaccinia virus vaccines may have potential for use in veterinary medicine.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002417:Cattle / Q000276:immunology*; D005818:Genetic Engineering; D007271:Injections, Intradermal / Q000662:veterinary; D011995:Recombination, Genetic; D012845:Sindbis Virus / Q000235:genetics* / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines