Plasma levels of 6-keto PGF1 alpha but not TxB2 increase in rats with peritonitis due to cecal ligation.
Acute, overwhelming sepsis or endotoxemia in experimental animals is associated with increased circulating levels of thromboxane (Tx)B2 (stable metabolite of TxA2) and 6-keto PGF1 alpha (stable metabolite of prostacyclin). The purpose of the present investigation was to determine the plasma prostanoid response to sepsis using an animal paradigm in which the septic process evolved more slowly than in previous similar studies. Bacterial peritonitis was induced in rats by cecal ligation (group B) or cecal ligation plus puncture with a 22-gauge needle (group C). Compared to sham-operated controls (group A), levels of immunoreactive 6-keto PGF1 alpha were significantly (p less than .05) elevated in group C rats at 6, 12, and 24 hr after surgery. At 48 hr after surgery, levels of this prostanoid were significantly (p less than .05) elevated in group B animals. In contrast, TxB2 levels were never significantly increased in septic (groups B and C) as compared to control (group A) rats. These data are consistent with results from several clinical studies and emphasize an important difference between the cecal ligation model and other experimental sepsis paradigms.
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood*; D000818:Animals; D002432:Cecum; D008026:Ligation; D008297:Male; D010538:Peritonitis / Q000097:blood*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D018805:Sepsis / Q000097:blood / Q000401:mortality; D013929:Thromboxane B2 / Q000097:blood*; D013997:Time Factors
D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha