In situ ultrastructural demonstration of cells bearing Ia antigens in the murine pancreas.
Although an important role has been postulated for class II major histocompatibility gene products in the rejection of transplanted islets, the identity of the cells bearing these molecules within pancreatic tissue has been the topic of some controversy. To clearly define the cell population(s) within the murine pancreas that express Ia antigens, perfused murine pancreata were processed for the ultrastructural in situ demonstration of Ia antigens. It was found that Ia antigen expression was restricted to mononuclear cells adjacent to capillaries throughout the exocrine and endocrine portions of the pancreas. Vascular endothelium and ductular epithelium did not express detectable amounts of Ia antigens.
D000818:Animals; D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology; D010179:Pancreas / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II