Genes specifying degradation of 3-chlorobenzoic acid in plasmids pAC27 and pJP4.
All of the structural genes for 3-chlorobenzoate degradation are clustered in a 4.2-kilobase (kb) region of plasmid pAC25 (or pAC27) in Pseudomonas putida. An approximate 10-kb DNA segment containing three structural genes for chlorocatechol metabolism present on plasmid pJP4 in Alcaligenes eutrophus shows homology with the above 4.2-kb region of pAC27. In spite of the detectable sequence homology in the structural genes present on both plasmids, the regulation of their expression seems quite different; unlike pAC27, structural rearrangements are prerequisite for efficient expression of the 3-chlorobenzoate genes on plasmid pJP4. Structural features such as stem-loop structures present on plasmid pJP4 are most likely the starting materials for such rearrangements.
D000421:Alcaligenes / Q000235:genetics* / Q000378:metabolism; D005075:Biological Evolution; D002723:Chlorobenzoates / Q000378:metabolism*; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism
D002723:Chlorobenzoates; C036427:3-chlorobenzoic acid