Inhibition of staphylococcal alpha-hemolysis by monoclonal antibodies against oligomer 12 S alpha-toxin.
Oligomer 12 S alpha-toxin as well as 3 S alpha-toxoid of Staphylococcus aureus induced the formation of monoclonal antibodies (mabs). Mabs against the 12 S alpha-toxin could be demonstrated in 31 and those against 3 S alpha-toxoid in 18 of 120 hybrid cell colonies. Each of these mab-preparations reacted with 12 S, 3 S alpha-toxin and 3 S alpha-toxoid. The reactions were more pronounced with the homologous than the heterologous toxin preparations. Mabs against 12 S alpha-toxin inhibited the hemolytic effects of native 3 S alpha-toxin as well or better than the respective polyclonal antisera.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D006460:Hemolysin Proteins; D006461:Hemolysis / Q000187:drug effects*; D006825:Hybridomas / Q000276:immunology; D007700:Kinetics; D051379:Mice; D013211:Staphylococcus aureus / Q000032:analysis
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin