Phosphorylation of nonhistone proteins during premature chromosome condensation in a temperature-sensitive mutant, tsBN2.
In tsBN2 cells, a temperature-sensitive (ts) mutant of the BHK21 cell line, with a ts-defect in its regulatory system for chromosome condensation, antigens that react with mitotic specific mouse monoclonal antibody MPM-2 were produced when premature chromosome condensation (PCC) was induced by a temperature shift. The polypeptides of antigens recognized by MPM-2 in tsBN2 cells with PCC were identical to those of antigens in mitotic cells. These antigens appeared concomitantly with chromosome condensation, which suggests that these mitotic-specific antigens may be related to chromosome condensation. As the production of mitotic-specific antigens was inhibited by W-7, a specific and potent antagonist of calmodulin, calmodulin may function in the mitotic phosphorylation of nonhistone protein.
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000096:biosynthesis / Q000276:immunology; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology / Q000378:metabolism*; D002877:Chromosomes, Human / Q000502:physiology*; D003101:Collodion; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007399:Interphase; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009154:Mutation; D010209:Paper; D010766:Phosphorylation; D013449:Sulfonamides / Q000494:pharmacology; D013696:Temperature
D000911:Antibodies, Monoclonal; D000941:Antigens; D002868:Chromosomal Proteins, Non-Histone; D013449:Sulfonamides; C017967:W 7; D003101:Collodion