Tubulin and benzimidazole-resistance in Trichostrongylus colubriformis (Nematoda).
Benzimidazole treatment produced greater effects on microtubule-dependent acetylcholinesterase secretion, the presence of microtubules in intestinal cells, and colchicine binding in susceptible compared with benzimidazole-resistant Trichostrongylus colubriformis. In addition, the binding of benzimidazoles was markedly reduced in preparations from the latter strain, indicating that the mechanism of resistance to benzimidazoles in this nematode involves a reduced affinity of tubulin for benzimidazoles.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001562:Benzimidazoles / Q000378:metabolism / Q000494:pharmacology*; D003078:Colchicine / Q000378:metabolism / Q000494:pharmacology; D004351:Drug Resistance; D008463:Mebendazole / Q000494:pharmacology; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D013827:Thiabendazole / Q000378:metabolism / Q000494:pharmacology; D014254:Trichostrongylus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism*
D001562:Benzimidazoles; D014404:Tubulin; C011321:oxibendazole; D008463:Mebendazole; D000110:Acetylcholinesterase; D013827:Thiabendazole; C100292:parbendazole; C011030:oxfendazole; D003078:Colchicine