Response of normal and diabetic rat aorta to deendothelialization in terms of 6-keto-prostaglandin F1 alpha synthesis and cholesterol accumulation.
The response of rat aorta to balloon catheter-induced denudation in terms of 6-keto-PGF1 alpha synthesis and cholesterol accumulation was studied in normal and streptozotocin-induced diabetic animals. Initial studies in control rats showed that 1 day after deendothelialization, aortic 6-keto-PGF1 alpha was significantly reduced (31%), and this was normalized at 4 days after injury. However, endothelial regeneration as indicated by Evans blue staining was complete only at 14 days after denudation. Diabetic aorta showed a more severe reduction of 6-keto-PGF1 alpha production (50%) at 1 day after deendothelialization, but this was also normalized at 4 days after injury. Deendothelialization did not increase the accumulation of cholesterol in diabetic aorta. It is concluded that (a) recovery of PGI2 production in deendothelialized aorta does not parallel endothelial regeneration, indicating substantial contribution of subendothelial layer, in rats and (b) short-term aortic injury in diabetic rat will not influence the arterial repair process and cholesterol accumulation and is unlikely to be a contributing factor to increased atherogenesis.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology*; D002404:Catheterization; D002784:Cholesterol / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D004727:Endothelium / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D015121:6-Ketoprostaglandin F1 alpha; D002784:Cholesterol