Phospholipids involved in specific binding of 12-O-(5-azido-2-nitrobenzoyl)phorbol-13-acetate to epidermal microsomes, a photolabeling study.
The preparation of 12-O-(5-azido-2-nitro)benzoylphorbol-13-acetate (NABPA) is described. It is used as a photoaffinity probe to study the biochemical components involved in the specific binding of phorbol esters to an epidermal particulate fraction (microsomes) from NMRI mice: without irradiation NABPA binds in a saturable and high affinity manner (KD = 12 nM; Rt = 2.6 pmol/mg protein) to microsomes; after irradiation (at 350 nm) specific photolabeling (representing specific binding of NABPA) is found of phospholipids (phosphatidyl-serine (PS) and -ethanolamine(PE)), but not of protein. The results are discussed in the context of protein kinase C being a receptor for phorbol esters.
D000345:Affinity Labels; D000818:Animals; D001386:Azides / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D008861:Microsomes / Q000378:metabolism*; D010703:Phorbol Esters / Q000378:metabolism*; D010704:Phorbols / Q000378:metabolism*; D010743:Phospholipids / Q000502:physiology*; D010782:Photolysis; D012867:Skin / Q000378:metabolism*; D014316:Tritium
D000345:Affinity Labels; D001386:Azides; D002273:Carcinogens; D010703:Phorbol Esters; D010704:Phorbols; D010743:Phospholipids; D014316:Tritium; C044835:12-O-(5-azido-2-nitrobenzoylphorbol)-13-acetate