Ultrastructural patterns of the alpha-naphthyl butyrate esterase activity in normal and leukaemic peripheral blood leucocytes.
The activity of alpha-naphthyl butyrate esterase was investigated at the ultrastructural level in normal human peripheral blood and in a few cases of hairy cell leukaemia, B-chronic lymphocytic leukaemia and acute monocytic leukaemia. A membrane reactivity was detected in most normal monocytes and lymphocytes. The activity in monocytes was very strong and was inhibited by NaF. It was NaF-resistant and less intense in lymphocytes. The reaction product was localized in the cytoplasm only in a small percentage of lymphocytes. In lymphocytes and monoblasts from pathological samples the pattern of reactivity was similar to that found in their normal counterparts, except for a lower intensity. The hairy cells showed a discrete distribution of the NaF-resistant reaction product on their cell surface. The different patterns of enzyme distribution are discussed critically.
D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D007943:Leukemia, Hairy Cell / Q000201:enzymology; D007945:Leukemia, Lymphoid / Q000201:enzymology; D007948:Leukemia, Monocytic, Acute / Q000201:enzymology; D007962:Leukocytes / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D012016:Reference Values
D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase