Interaction between citrate synthase and thiolase.
Thiolase, a mitochondrial matrix enzyme which produces CoASAc from fatty acids, is shown to interact with citrate synthase, the mitochondrial matrix enzyme responsible for CoASAc utilization. The interaction is demonstrated in three ways: the two enzymes co-precipitate in polyethylene glycol; thiolase causes a change in the fluorescence anisotropy of labeled citrate synthase; and the two enzymes co-elute in gel permeation chromatography. The interactions are shown to be specific by the use of enzymes not metabolically related to citrate synthase.
D000102:Acetyl-CoA C-Acyltransferase / Q000378:metabolism*; D000217:Acyltransferases / Q000378:metabolism*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D005454:Fluorescence Polarization; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000378:metabolism; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D013389:Succinate-CoA Ligases / Q000378:metabolism
D008291:Malate Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase; D000217:Acyltransferases; D000102:Acetyl-CoA C-Acyltransferase; D002332:Carnitine O-Acetyltransferase; D002950:Citrate (si)-Synthase; D001219:Aspartate Aminotransferases; D007652:Oxo-Acid-Lyases; D013389:Succinate-CoA Ligases