Partial characterization of n-butanol-solubilized rejection-type antigens of syngeneic murine colon tumors.
Previous investigation of the transplantation immunity of 2 cultured murine colon lines of BALB/c origin, C-C36 and C-C26, showed these tumor lines to be immunogenic against individual tumors and to have possibly cross-reactive, tumor-rejection-type antigens. For characterization of the molecular features of tumor-rejection antigens expressed on the colon tumor cells, n-butanol was used for the extraction of rejection-type antigens from tumor cells and immunogenic molecules were analyzed on transplantation immunity. The data demonstrated that extraction of the rejection-type antigens from C-C36 and C-C26 surface membrane without cellular lysis was possible with n-butanol treatment of these cells, and immunogenic activities of these extracts from C-C36 and C-C26 cells were more potent than those of nonionic detergent Nonidet P40 extracts in the tumor-rejection assays. The extracts were partially characterized by chromatographic separation on Sephadex G-200 gel filtration and lectin-affinity chromatography. It was suggested that the C-C36 antigens responsible for tumor-rejection activity against the same tumor cells had a molecular weight range of approximately 150,000 to 250,000 (fraction II) in the presence of 5 mM EDTA and had been eluted into unbound fractions to lens culinaris lectin on affinity chromatography. Moreover, immunization of mice with antigens from the same fractions (fraction II) of n-butanol extracts of C-C26 tumor on the gel filtration could induce the resistance against challenged C-C36 as well as against challenged C-C26 tumor growth. These results may indicate that solubilized tumor-rejection-type antigens found in C-C36 and C-C26 colon tumors have a size similar to that of the molecules and that cross-reacting, rejection-type antigens between these cells are the products of the same gene clusters or somatic derivatives of a single gene.
D020001:1-Butanol; D000230:Adenocarcinoma / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D000440:Butanols; D002277:Carcinoma / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D006084:Graft Rejection; D006649:Histocompatibility Antigens / Q000032:analysis*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D017830:Octoxynol; D011092:Polyethylene Glycols; D012995:Solubility; D014020:Tissue Extracts / Q000276:immunology; D014181:Transplantation Immunology
D000951:Antigens, Neoplasm; D000440:Butanols; D006649:Histocompatibility Antigens; D014020:Tissue Extracts; C029237:tumor-associated transplantation antigen; D011092:Polyethylene Glycols; D020001:1-Butanol; D017830:Octoxynol; C010615:Nonidet P-40