A potential microneurosurgical method for the manipulation of the developing nervous system of the postimplantation mouse embryo in utero. I. Injection methods.
A microsurgical method for the injection of cells into the postimplantation mouse embryo whilst it is in utero is described. Specific regions of the 9.5-day-old mouse embryo brain have been injected with marker substances, such regions including the intraventricular cavity of the telencephalon and the trigeminal facialis ganglion bulge of the forebrain. Histological study of the injected embryos shows that the injection method is precise and able to avoid damage to other nearly embryonic tissues. The method should have wide application in the study of questions of normal cell lineage, neoplasia, teratology, and toxicology.
D000818:Animals; D001921:Brain / Q000196:embryology*; D004622:Embryo, Mammalian / Q000601:surgery*; D004626:Embryology / Q000379:methods*; D047108:Embryonic Development; D005260:Female; D007267:Injections / Q000379:methods; D007276:Injections, Intraventricular / Q000379:methods; D051379:Mice; D008809:Mice, Inbred C3H; D008866:Microsurgery / Q000379:methods; D009493:Neurosurgery / Q000379:methods*; D011247:Pregnancy; D014180:Transplantation / Q000379:methods