Transferrin in isolated cells from rat duodenum and jejunum.
Mucosal transferrin was determined as transferrin-like immunoreactivity (TLIR) by means of a 2-site immunoradiometric assay (IRMA). Scraped-off mucosal tissue as well as isolated mucosal cells from the duodenum and jejunum of normal and iron-deficient rats before and after a washing procedure were examined. In iron-deficient rats there was about twice as much TLIR in scraped-off mucosal tissue as in the untreated animals. In the duodenum and jejunum of normal and iron-deficient rats, TLIR contents of the isolated cells in the magnitude of 320-510 ng/mg dry weight were found. Washing isolated cells three times in ice-cold Hank's solution resulted in a nearly tenfold decrease of TLIR content in all groups. In contrast the cells' RNA content remained unchanged.
D000818:Animals; D004386:Duodenum / Q000166:cytology*; D007413:Intestinal Mucosa / Q000032:analysis*; D000090463:Iron Deficiencies; D007583:Jejunum / Q000166:cytology*; D051381:Rats; D014168:Transferrin / Q000032:analysis*
D014168:Transferrin