Immobilized enzymes in organic synthesis.
The immobilization of enzymes and cells by different methods and the possible stabilization of immobilized preparations are discussed. An outlook on 'second generation enzyme technology', which involves immobilized multi-enzyme systems and coenzymes, is given with examples: the immobilization of dehydrogenases with their active sites facing one another, and systems containing NAD(H) coenzymes immobilized by coupling to dextran (in an enzyme electrode), to polyethylene glycol (in a membrane reactor), or to enzymes themselves. The use of immobilized enzymes to synthesize peptides and disaccharides is described.
D055598:Chemical Phenomena; D002621:Chemistry; D003067:Coenzymes / Q000378:metabolism; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology; D011092:Polyethylene Glycols
D003067:Coenzymes; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009243:NAD; D011092:Polyethylene Glycols; D010088:Oxidoreductases