Determination of citalopram, amitriptyline and clomipramine in plasma by reversed-phase high-performance liquid chromatography.
The determination of citalopram, amitriptyline, clomipramine and their desmethyl metabolites after alkaline diethyl either extraction from plasma is achieved by high-performance liquid chromatography using two internal standards and mu Bondapak C18 as stationary phase. Elution is carried out isocratically at 0.5 or 1 ml/min with a mixture of acetonitrile-potassium dihydrogen phosphate-distilled water (45:50:5). Detection is monitored by absorption at 254 nm. The detection limit is less than 5 ng/ml for each compound. The coefficients of variation are between 1.3% and 9.4% for 8-360 ng/ml. Interference from 22 possible co-medications is discussed. The technique can be used for therapeutic monitoring of these antidepressants as well as in analytical toxicology.
D000639:Amitriptyline / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D015283:Citalopram; D002997:Clomipramine / Q000097:blood*; D006801:Humans; D011437:Propylamines / Q000097:blood*; D012702:Serotonin Antagonists / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D011437:Propylamines; D012702:Serotonin Antagonists; D015283:Citalopram; D000639:Amitriptyline; D002997:Clomipramine