Transcytosis of ferritin and increased production of subendothelial matrix components by aortic endothelial cells during the development of hypertension.
The aortic intima of the Spontaneously Hypertensive Rat underwent dramatic structural alterations with respect to the subendothelial space coincident with the rise in arterial pressure. This space became increasingly widened from four to 24 weeks, and exhibited a dense accumulation of granular material, resembling a complex reticular basement membrane. To determine if this alteration was due to an abnormality in endothelial transcytosis, rats of four, ten and 24 weeks of age were studied using ferritin as a tracer. In the SHR, vesicular transport of injected ferritin was significantly increased at all ages studied compared to the WKY, but decreased to prehypertensive levels by 24 weeks of age. This occurred without a change in the density of vesicles and caveolae within the cells. Despite the decrease in transcytosis from 10 to 24 weeks the alterations of the subendothelial space continued indicating that the endothelium may play a role in the synthesis and deposition of the dense extracellular matrix.
D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000473:pathology; D001693:Biological Transport, Active; D004727:Endothelium / Q000378:metabolism / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D005293:Ferritins / Q000378:metabolism*; D006973:Hypertension / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D005293:Ferritins