Carbachol stimulation of phosphatidic acid synthesis: competitive inhibition by pirenzepine in synaptosomes from rat cerebral cortex.
Carbachol stimulated phosphatidic acid synthesis in cholinergically enriched synaptosomes from rat cerebral cortex. Increasing concentrations of pirenzepine (10-1000 nM) produced parallel concentration-response curves to carbachol which were shifted to the right. A pA2 value for pirenzepine of 8.4 +/- 0.3 was obtained from Schild analysis. We hypothesize that high affinity pirenzepine binding to M1 receptors is coupled to phosphatidic acid synthesis in the rat cerebral cortex.
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology*; D002217:Carbachol / Q000037:antagonists & inhibitors*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010712:Phosphatidic Acids / Q000096:biosynthesis*; D010890:Pirenzepine; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
D001570:Benzodiazepinones; D010712:Phosphatidic Acids; D011976:Receptors, Muscarinic; D010890:Pirenzepine; D002217:Carbachol