Enhanced activity of the macrophage-like cell line J774.1 following exposure to gamma radiation.
Exposure of the macrophage-like cell line J774.1 to 20 gray of cobalt-60 gamma radiation resulted in a block of tritiated thymidine incorporation, along with an increase in cell "activation," as assessed by increases in lysosomal enzyme and ectoenzyme content, PMA-induced H2O2 production, and NBT staining, ingestion of E(IgG), spreading, and membrane ruffling. These changes are evident within 1 day postradiation and peak at 4 days postradiation.
D000818:Animals; D002448:Cell Adhesion / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000528:radiation effects; D005720:Gamma Rays; D006861:Hydrogen Peroxide / Q000378:metabolism; D008262:Macrophage Activation / Q000528:radiation effects*; D008264:Macrophages / Q000201:enzymology / Q000528:radiation effects*; D051379:Mice; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis / Q000528:radiation effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D009580:Nitroblue Tetrazolium; D004247:DNA; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate