Typing for HLA class II at the product level.
Class II antigens were isolated from consanguineous homozygous typing cells by sequential immunoprecipitation with the MoAbs: 7.3.19.1 (anti-DRw52-like), B.8.11.2 (anti-DR backbone) and 7.5.10.1 (anti-HLA class II backbone). Depending on the DR serotype of the cell line used, two or three families of class II antigens could be isolated [1]. For each homozygous typing cell the different families of class II antigens were analysed on 1D-IEF gels. Charge heterogeneity showed that the different haplotypes are distinct in electrophoretic beta chain patterns. For each homozygous typing cell at least one beta chain was observed that possessed a haplotype unique pI. This means that typing for HLA class II at the product level is possible.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D005260:Female; D006684:HLA-DR Antigens; D006579:Heterozygote; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006720:Homozygote; D006801:Humans; D007526:Isoelectric Point; D008297:Male; D010375:Pedigree
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II