Human dioxin-inducible cytochrome P1-450: complementary DNA and amino acid sequence.
Induction of cytochrome P1-450 has been linked to susceptibility to certain chemically induced cancers in mouse and man. Treatment of the human cell line MCF-7 with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) results in high levels of aryl hydrocarbon (benzo[a]pyrene) hydroxylase (P1-450) activity. This cell line was used to isolate a human P1-450 full-length complementary DNA (cDNA) clone. The cDNA is 2566 nucleotides in length, encodes a polyadenylated messenger RNA (2.8 kilobases in length), and has a continuous reading frame producing a protein with 512 residues (molecular weight, 58,151). The human P1-450 cDNA and protein are 63 percent and 80 percent similar to mouse P1-450 cDNA and protein, respectively. Whereas the mouse TCDD-inducible P-450 gene subfamily has two members (P1-450 and P3-450), the human TCDD-inducible gene subfamily appears to have only one gene (P1-450).
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004147:Dioxins / Q000494:pharmacology*; D004790:Enzyme Induction; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D011817:Rabbits
D002273:Carcinogens; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D004247:DNA; D003577:Cytochrome P-450 Enzyme System