Effects of gonadectomy and sex hormones on the levels of noradrenaline and dopamine in rat hypothalamus.
The levels of noradrenaline and dopamine were determined in the homogenates of the hypothalamus of rats of either sex. The determinations were done in intact rats, after sham gonadectomy, 6 and 9 weeks after gonadectomy, and in gonadectomized rats receiving 6 weeks after gonadectomy one dose of oestradiol cypionate (females) or testosterone cypionate (males). Catecholamines were determined fluorimetrically. The changes of the determined catecholamines differed in the hypothalamic homogenates in males and females after gonadectomy. Following orchidectomy the noradrenaline level rose, while after ovariectomy the level of this catecholamine decreased. Contrary to this, in ovariectomized rats the dopamine level was significantly raised after the operation. This change was reversible as observed after administration of oestradiol cypionate. Orchidectomy and testosterone cypionate injection had no effect on the dopamine level in the hypothalamus. The role of these catecholamines in the processes connected with the regulation of the hypothalamus-hypophysis-gonad axis is discussed.
D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D009919:Orchiectomy; D010052:Ovariectomy; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology
D013739:Testosterone; D004958:Estradiol; C007630:estradiol 17 beta-cypionate; C016131:testosterone 17 beta-cypionate; D004298:Dopamine; D009638:Norepinephrine