Reovirus hemagglutinin mRNA codes for two polypeptides in overlapping reading frames.
Human reovirus s1 mRNA, which codes for the viral hemagglutinin, also directs the synthesis of a previously unrecognized polypeptide of molecular mass 14 kDa in reticulocyte and wheat germ extracts. Hybrid-arrest of translation by selected restriction fragments of cloned S1 DNA indicated that synthesis of the 14-kDa polypeptide initiates at the second AUG. This was confirmed by NH2-terminal sequence analyses. The coding sequence for the 14-kDa polypeptide thus lies entirely within the hemagglutinin gene but in a different reading frame. Although not found in virions, the 14-kDa polypeptide apparently is formed in virus-infected mouse L cells, as demonstrated by comparison of [35S]methionine-labeled polypeptides in cell extracts with the corresponding in vitro products.
D000595:Amino Acid Sequence; D002474:Cell-Free System; D006389:Hemagglutinins, Viral / Q000235:genetics*; D012089:Mammalian orthoreovirus 3 / Q000235:genetics*; D010446:Peptide Fragments / Q000032:analysis; D014176:Protein Biosynthesis; D012330:RNA, Double-Stranded / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D006389:Hemagglutinins, Viral; D010446:Peptide Fragments; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins