5-Methyl-2-thiouridine in the tRNA of Candida tropicalis and its localization in lysine tRNA.
35S incorporation studies showed that Candida tropicalis tRNA contained two thionucleosides, one of which was identified as 5-methyl-2-thiouridine. The other thionucleoside was alkali labile, and it appeared to be an ester. Pulse-chase experiments suggested that the two thionucleosides were structurally related. 5-Methyl-2-thiouridine was present in one of the lysine tRNAs. This is the first report of the presence of this nucleoside in a yeast tRNA.
D002175:Candida / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis*; D012343:RNA, Transfer / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*; D013872:Thionucleosides / Q000032:analysis* / Q000096:biosynthesis; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000032:analysis
D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D013872:Thionucleosides; D013891:Thiouridine; C022856:2-thioribothymidine; D012343:RNA, Transfer