Anti-dental caries effect in rats and man of a bacteriocin purified from the oral bacterium Streptococcus mutans C3603.
Specific pathogen-free rats infected with Streptococcus mutans PS-14 (serotype c) and fed a cariogenic diet containing 380 parts/10(6) of bacteriocin, purified from Strep. mutans C3603, developed significantly fewer carious lesions than controls infected with Strep. mutans and fed the same diet without bacteriocin. The caries-inhibitory effect was 58.7 per cent. Oral appliances fixed with bovine enamel slabs were worn by volunteers to test bacteriocin C3603. The enamel slabs were treated with 500 parts/10(6) bacteriocin in 3 per cent sucrose solution for 10 min, 4 times daily, or with 3 per cent sucrose solution, 3 times daily, for 10 min and just before bedtime with 500 parts/10(6) bacteriocin once for 1 min. The bacteriocin exerted a strong inhibition of cariogenicity of sucrose.
D000818:Animals; D001430:Bacteriocins / Q000494:pharmacology*; D002327:Cariostatic Agents; D004037:Diet, Cariogenic; D006801:Humans; D051381:Rats; D011919:Rats, Inbred Strains; D013295:Streptococcus mutans / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D014070:Tooth / Q000187:drug effects
D001430:Bacteriocins; D002327:Cariostatic Agents; D013395:Sucrose