Template-directed synthesis of oligoadenylates using cyanogen bromide and diiminosuccinonitrile.
Cyanogen bromide was found to be a highly effective reagent for the coupling of oligoadenylates on a polyuridylic acid template in aqueous solution. Imidazole and divalent metal ions were required for the reaction. Hexaadenylic acid was converted to (pA)12, (pA)18, (pA)24, (pA)30 and (pA)36 in the presence of 0.01 M Co2+ and imidazole. When the reaction was carried out at 25 degrees C for 3 days, the oligomers were obtained in a 34% overall yield.
D000227:Adenine Nucleotides / Q000138:chemical synthesis*; D002413:Cations, Divalent; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D007202:Indicators and Reagents; D009570:Nitriles; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D013329:Structure-Activity Relationship; D013698:Templates, Genetic
D000227:Adenine Nucleotides; D002413:Cations, Divalent; D007202:Indicators and Reagents; D009570:Nitriles; D009843:Oligoribonucleotides; C047926:diiminosuccinonitrile; C023505:2',5'-oligoadenylate; D003488:Cyanogen Bromide