Far-field 2.45 GHz irradiation system for cellular monolayers in vitro.
A 2.45-GHz microwave exposure facility was developed for long-term TEM irradiation of cellular monolayers. Culture flasks with cells attached to the inside bottom surface were filled with medium, submerged in a 60 X 60 X 12-cm water bath on the field central axis, and exposed in the far-field 2 m below the ceiling-mounted antenna. A quarter-wave transformer plate increased the power transmitted into the water bath, and treatment temperatures were maintained by closed circulation with an external temperature control reservoir. Power density mapped below the quarter-wave plate indicated uniform TEM fields in the 25 X 25-cm region where flasks were located. With 1 kW of forward power to the antenna, the SAR [W/kg] = 45 exp(-0.607d) where d [cm] is the depth in water at any point within this area.
D000818:Animals; D002151:Calorimetry; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects*; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D008872:Microwaves