Partial purification and initial characterization of a novel differentiation factor for mouse myeloid leukemia cells.
We found cells spontaneously differentiated from mouse myeloid leukemia M1 cells were producing a strong differentiation factor in culture medium and established a method to prepare a large quantity of conditioned medium containing the differentiation factor. The factor purified over 4,000-fold from the conditioned medium showed a single peak due to a peptide on a TSK 3000PW column which was coincident with differentiation activity. The molecular weight of the factor estimated by high-performance gel filtration chromatography was 1,300, which is remarkably lower than the values reported for protein differentiation factors reported thus far. M1 cells were induced to differentiate into macrophage-like cells by the factor.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D006023:Glycoproteins / Q000302:isolation & purification*; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D015470:Leukemia, Myeloid, Acute / Q000473:pathology* / Q000503:physiopathology; D008222:Lymphokines; D051379:Mice; D008970:Molecular Weight
D003470:Culture Media; D006023:Glycoproteins; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines