Effects of dopamine and vasopressin on histamine-induced increases in macromolecular permeability in the canine forelimb.
Local intra-arterial infusions of histamine, 16 micrograms base/min, for 60 minutes produced increases in lymph flow, lymph total protein concentration, the lymph/plasma total protein ratio, and weight in canine forelimbs perfused at constant flow. The weight gains were far greater than can be accounted for by an increase in vascular volume, and must, therefore, be attributed to edema formation. Treatment with dopamine (2, 4, or 8 micrograms base/min) or arginine vasopressin (AVP) initially produced an increase in perfusion pressure reflecting constriction of the forelimb vasculature. AVP prevented the histamine-induced increase in lymph flow, lymph total protein concentration, the L/P total protein ratio, and weight gain. In contrast, treatment with DA at three different dose levels failed to affect the histamine response. The increases in lymph flow, lymph total protein concentration, lymph/plasma total protein ratio, and weight were comparable to that produced by histamine in the absence of DA. These data fail to provide evidence for DA mediated regulation of macromolecular permeability, and suggest that endothelial cells either do not contain DA receptors, or that their activation does not affect macromolecular permeability.
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004285:Dogs; D004298:Dopamine / Q000008:administration & dosage / Q000494:pharmacology*; D004487:Edema / Q000139:chemically induced; D005260:Female; D005552:Forelimb / Q000098:blood supply / Q000187:drug effects / Q000503:physiopathology*; D006632:Histamine / Q000008:administration & dosage / Q000494:pharmacology*; D007261:Infusions, Intra-Arterial; D008196:Lymph / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D046911:Macromolecular Substances / Q000378:metabolism; D008297:Male; D009929:Organ Size; D014667:Vasopressins / Q000008:administration & dosage / Q000494:pharmacology*; D014690:Venous Pressure
D046911:Macromolecular Substances; D014667:Vasopressins; D006632:Histamine; D004298:Dopamine