[Mucosa dimensions and cell proliferation in intestinal organ cultures with and without inhibition of cholesterol synthesis].
The changes in the structure and proliferative activity of rabbit ileal mucosa have been studied with morphometric and stathmokinetic techniques during 24 h organ culture. Under control conditions the villus base diameter and villus height decreased by 34 and 53%, respectively, whereas the number of villi per mm2 remained constant. The reduction of crypt depth was less pronounced than the loss in villus height, leading to a decrease in the villus height to crypt depth ratio from 3.4 to 2.3. The likely reason for this culture induced hypoplasia was a significant drop in mitotic activity as early as 2 h after starting the culture. Inhibition of HMG-CoA reductase, the key enzyme of cholesterogenesis, lead to a further reduction in mucosal dimensions and a near complete suppression of crypt cell regeneration. These data imply that despite the well-known preservation of differentiated function mucosal cell renewal during organ culture is impaired.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007082:Ileum / Q000166:cytology / Q000187:drug effects; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects; D008148:Lovastatin; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D009281:Naphthalenes / Q000494:pharmacology; D009924:Organ Culture Techniques; D011817:Rabbits
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D009281:Naphthalenes; D002784:Cholesterol; D008148:Lovastatin; D006903:Hydroxymethylglutaryl CoA Reductases
Mukosadimensionen und Zellproliferation in intestinaler Organkultur mit und ohne Hemmung der Cholesterinsynthese.