Computerized calculation of in vitro generation of ATP and creatine phosphate induced by respiration in human muscle mitochondria.
A computer program is presented which calculates the action of adenylate kinase and creatine kinase during the process of adenosine-5'-triphosphate generation by the mitochondria in human skeletal muscle 600 X g supernatants. The results show that the mass action ratios of the two kinase reactions are not influenced by incubation time or decreased rate of oxidative phosphorylation as a result of mitochondrial dysfunction. The program is useful for establishing optimum initial adenosine-5'-diphosphate and creatine concentrations in the assay medium.
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000263:Adenylate Kinase / Q000378:metabolism; D003201:Computers; D003402:Creatine Kinase / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008931:Mitochondria, Muscle / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010725:Phosphocreatine / Q000096:biosynthesis*; D012984:Software
D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D000263:Adenylate Kinase