Stimulatory and inhibitory activities of lung-conditioned medium on the growth of normal and neoplastic cells in vitro.
Lung-conditioned medium (LCM) was obtained by incubation of BALB/c mouse lung tissue fragments in serum-free Eagle medium for 48 hours and subsequent separation by dialysis or chromatography on a Sephadex G-75 column. LCM fractions were tested for their ability to modulate proliferation of normal human endothelial cells and neoplastic cells (N2a, MCF, HEp-2) in vitro as assessed by plating efficiency and tritiated thymidine incorporation assays. It was found that LCM contained two kinds of factors, either stimulating (molecular weight: 50,000-70,000) or inhibiting (molecular weight: 12,000-20,000 and 3,000-5,000) cell proliferation.
D000818:Animals; D001943:Breast Neoplasms; D002455:Cell Division; D002460:Cell Line; D002850:Chromatography, Gel; D003470:Culture Media; D003956:Dialysis; D004727:Endothelium; D005260:Female; D006131:Growth Inhibitors / Q000502:physiology; D006133:Growth Substances / Q000032:analysis / Q000502:physiology*; D006801:Humans; D007822:Laryngeal Neoplasms; D008168:Lung / Q000032:analysis / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009369:Neoplasms / Q000473:pathology*; D009447:Neuroblastoma
D003470:Culture Media; D006131:Growth Inhibitors; D006133:Growth Substances