Changes in DNA capacity for actinomycin-D binding in nuclei of interphase cells.
3H-AMD binding to DNA in interphase nuclei was tested on asynchronous and synchronous LS/BL cell populations under physiological conditions and after exposure to gamma rays (60Co). 3H-AMD binding to DNA in an asynchronous cell population appeared to be nearly constant and independent of 3H-AMD concentration. However, in comparing individual cells, a great variability could be observed. In synchronized cells the DNA accessibility for 3H-AMD binding changed in the course of the cell cycle, with a maximum occurring at the late G1-phase (13.95 X 10(-12) mumol/nucleus) and a minimum at the late G2-phase (2.63 X 10(-12) mumol/nucleus). In irradiated cells the DNA capacity for 3H-AMD binding was growing with the increasing dose (5-80 Gy) from 4.9 to 11.2 X 10(-12) mumol 3H-AMD/nucleus.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000378:metabolism*; D003609:Dactinomycin / Q000378:metabolism*; D007399:Interphase; D007700:Kinetics; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D014316:Tritium
D004273:DNA, Neoplasm; D014316:Tritium; D003609:Dactinomycin