Glycolysis and methylaminoisobutyrate uptake in rat-1 cells transfected with ras or myc oncogenes.
A high rate of aerobic glycolysis was catalyzed by rat-1 cells transfected with a ras oncogene (ras cells); rat-1 cells and rat-1 cells transfected with myc oncogene (myc cells) showed a low rate of glycolysis that was increased after exposure of the cells to type B transforming growth factor (TGF-beta). The uptake of radioactive methylaminoisobutyric acid or L-methionine via system A of amino acid transport also was accelerated after exposure of these cells to TGF-beta, with the myc cells being most sensitive and the ras cells least sensitive. Methionine was found to be a potent inhibitor of glycolysis in ras cells as well as in rat-1 or myc cells that were exposed to TGF-beta. We propose a relationship between the product of the ras oncogene (p21) and the protein(s) induced by exposure to TGF-beta.
D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts; D006019:Glycolysis / Q000187:drug effects; D008715:Methionine / Q000494:pharmacology; D009857:Oncogenes; D010455:Peptides / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051381:Rats; D014162:Transfection; D015291:Transforming Growth Factors
D000621:Aminoisobutyric Acids; D010455:Peptides; D010982:Platelet-Derived Growth Factor; C017911:2-(methylamino)isobutyric acid; D015291:Transforming Growth Factors; D003513:Cycloheximide; D008715:Methionine