Partial characterization of an erythropoiesis inhibitory factor.
An inhibitory factor of erythropoiesis, obtained from normal human urine, is indicated to be a complex of a fragment of alpha 1-acid glycoprotein and prostaglandin F2 alpha. Immunoelectrophoresis reveals two protein components in the EIF complex which separate during acrylamide gel electrophoresis. A gamma-globulin (MW 185,000) is a carrier of the complex. A fragment of alpha 1-acid glycoprotein (MW 9300) retains the inhibitory factor, PGF2 alpha. Noncovalent forces bind the PGF2 alpha to the protein, and PGF2 alpha can be extracted with benzene.
D001798:Blood Proteins / Q000652:urine*; D003377:Countercurrent Distribution; D015237:Dinoprost; D006801:Humans; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D011460:Prostaglandins F / Q000652:urine
D001798:Blood Proteins; D011460:Prostaglandins F; C007627:erythropoiesis inhibiting factors; D015237:Dinoprost