Decapreno-beta-carotene as an internal standard for the quantification of the hydrocarbon carotenoids by high-performance liquid chromatography.
The application of decapreno-beta-carotene as an internal standard in quantification of the hydrocarbon carotenoids extracted from raw carrots has been thoroughly examined. Decapreno-beta-carotene is a C50 beta-carotene that has most of the requirements of an internal standard and it can be commercially synthesized in high purity. An isocratic high-performance liquid chromatographic (HPLC) system has been developed that separated all-trans-alpha-carotene, all-trans-beta-carotene, and its 15,15'-cis-isomer from this internal standard. Quantitative determination of the hydrocarbon carotenoids in carrots by HPLC using the internal standard technique gave values for alpha- and beta-carotene similar to those obtained from alpha- and beta-carotene standards alone.
D002338:Carotenoids / Q000031:analogs & derivatives* / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D010936:Plant Extracts / Q000032:analysis; D012015:Reference Standards; D013055:Spectrophotometry, Infrared; D014675:Vegetables / Q000032:analysis; D019207:beta Carotene / Q000031:analogs & derivatives*
D007202:Indicators and Reagents; D010936:Plant Extracts; D019207:beta Carotene; D002338:Carotenoids; C047146:decapreno-beta-carotene