Morphological changes in CHO and VERO cells treated with T-2 mycotoxin. Correlation with inhibition of protein synthesis.
Exposure of Chinese hamster ovary and African green monkey kidney cells to T-2 mycotoxin resulted in several morphological changes which were related to inhibition of protein synthesis, the basic in vitro mechanism of action of the toxin. These changes, which occurred in both cell types, included disassociation of polysomes and mitochondrial cristae alterations. In addition, CHO cells displayed membrane bleb formations similar to those found in CHO cells after exposure to established inhibitors of protein synthesis, puromycin and anisomycin. Blebs could be either a result of protein synthesis inhibition or a non-specific early pathological response. Bleb formations were not observed in VERO cells under any experimental condition.
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000648:ultrastructure; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D011132:Polyribosomes / Q000648:ultrastructure; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology*; D013605:T-2 Toxin / Q000494:pharmacology*
D012717:Sesquiterpenes; D011691:Puromycin; D000841:Anisomycin; D013605:T-2 Toxin