Amiloride in differentiation and commitment of Friend erythroleukemia cells.
Dimethylsulfoxide (DMSO) converts almost all of the undifferentiated murine erythroleukemia cells (MEL or Friend cells, clone 745A) in a culture to differentiated cells that contain high levels of hemoglobin and that stop growing after a limited number of cell divisions. Contrary to other reports--that amiloride strongly inhibits DMSO-induced differentiation in MEL cells--in this laboratory, inhibition by amiloride, tested with DMSO over a range of concentrations in two kinds of media and at various cell densities, was found to be only weak or absent. Similarly, amiloride did not inhibit induction by N,N'-hexamethylene bis-acetamide (HMBA). As expected from previous findings with other cell systems, amiloride inhibited protein synthesis and cell multiplication.
D000081:Acetamides / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001560:Benzidines / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005622:Friend murine leukemia virus; D006454:Hemoglobins / Q000032:analysis; D004915:Leukemia, Erythroblastic, Acute / Q000648:ultrastructure*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D014176:Protein Biosynthesis
D000081:Acetamides; D001560:Benzidines; D006454:Hemoglobins; C029876:benzidine; D000584:Amiloride; C014026:hexamethylene bisacetamide; D004121:Dimethyl Sulfoxide