Human c-myc and N-ras expression during induction of HL-60 cellular differentiation.
The genome of the human HL-60 promyelocytic leukemia cell contains amplified c-myc sequences and the transforming N-ras oncogene. The present study has monitored c-myc and N-ras expression in HL-60 cells during induction of myeloid and monocytic differentiation with dimethyl sulfoxide, hexamethylene bisacetamide, 12-O-tetradecanoylphorbol-13-acetate and 1,25 dihydroxy-vitamin D3. The results demonstrate that induction of HL-60 differentiation is associated with decreases in c-myc RNA, while there is little if any effect on expression of the N-ras gene. Although the diminution in c-myc expression occurred as an early event in the induction of HL-60 differentiation, the rate of decrease in c-myc transcripts varied with respect to cessation of proliferation. Thus, the appearance of the mature phenotype and loss of proliferative capacity are associated with declines in c-myc RNA, while these events appear to occur in the absence of significant alterations in N-ras expression.
D000081:Acetamides / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D009857:Oncogenes / Q000187:drug effects; D012334:RNA, Neoplasm / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000081:Acetamides; D002273:Carcinogens; D012334:RNA, Neoplasm; D002117:Calcitriol; C014026:hexamethylene bisacetamide; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide