Opposite effects of a glucocorticoid and an immunostimulating agent on prostaglandin production by two different cell types.
Studies were performed to investigate the effects of several agents known to modulate wound healing on prostaglandin production by mouse embryo fibroblasts and adult thymic phagocytic cells in culture. Dexamethasone (10(-6)M) induced in both cell types a significant inhibition of the production of PGE2 and 6 keto PGF1 alpha, and a moderate inhibition of PGF2 alpha. Using an antiglucocorticoid compound, RU 38486, we were able to demonstrate that the inhibition of PG secretion represents a classical receptor-mediated effect of the steroid. In contrast, LPS and RU 41740 (5 micrograms/ml) induced a significant stimulation of PGE2 and 6-keto-PGF1 alpha secretion in the two types of cells. These results suggest that agents which modulate in different ways the process of tissue repair have opposite effects on PG production by cells involved in inflammatory and/or immunological reactions.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001426:Bacterial Proteins; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D015237:Dinoprost; D015232:Dinoprostone; D004336:Drug Antagonism; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000494:pharmacology*; D051379:Mice; D010586:Phagocytes / Q000187:drug effects / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D013929:Thromboxane B2 / Q000096:biosynthesis*; D013931:Thromboxanes / Q000096:biosynthesis*; D013950:Thymus Gland / Q000166:cytology
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D006023:Glycoproteins; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D013931:Thromboxanes; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C037567:glycoprotein, Klebsiella pneumoniae; D003907:Dexamethasone; D015237:Dinoprost; D015232:Dinoprostone