Nucleotide sequence of the respiratory syncytial virus phosphoprotein gene.
A cDNA library was prepared from poly(A)+ RNA extracted from respiratory syncytial (RS) virus-infected HEp-2 cells. A recombinant plasmid, pRSP68, encoding the RS virus phosphoprotein gene was identified by translation in vitro of hybrid-selected mRNA. The cloned cDNA insert of 1 kb was sequenced and a polypeptide of 241 amino acids with a molecular weight of 27 166 was deduced from the sequence. The protein was relatively rich in polar amino acids and devoid of both cysteine and tryptophan. A second short open reading frame with a coding potential of 65 amino acids was identified and overlapped the 3' terminus of the phosphoprotein gene by 34 bases.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D002460:Cell Line; D003062:Codon; D004247:DNA; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D000596:Amino Acids; D003062:Codon; D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA