The two intervening sequences of human beta- and gamma-globin pre-mRNAs are excised in a preferred temporal order in vitro.
Splicing of complete synthetic human beta- and gamma-globin mRNA precursors (pre-mRNAs) follows a preferred pathway in vitro. In almost all molecules, excision of the first intervening sequence (IVS) is completed before excision of the second IVS initiates. However, in a very small fraction of molecules the order of IVS excision is reversed.
D001483:Base Sequence; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D009694:Nucleic Acid Precursors / Q000235:genetics*; D010957:Plasmids; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D013698:Templates, Genetic; D013997:Time Factors; D014158:Transcription, Genetic
D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D005914:Globins