Plasmid transfer and behaviour in Acinetobacter calcoaceticus EBF65/65.
At least one plasmid from each of the incompatibility groups B, C, FIV, H2/S, I alpha, I delta, P, W and X was shown to be capable of transfer from Escherichia coli K12 to Acinetobacter calcoaceticus EBF65/65. Transfer was influenced by the presence of pAV2 (thought to encode a restriction-modification system) in the recipient strain; however, not all plasmids belonging to a particular incompatibility group behaved identically. All plasmids were unstable to varying degrees in A. calcoaceticus EBF65/65, but under suitable conditions were capable of transfer to further strains of EBF65/65 and re-transfer to E. coli K12. Of 40 recently isolated trimethoprim R plasmids 31 transferred successfully from E. coli K12 to A. calcoaceticus EBF65/65, but 17 of these 31 required the introduction of a second mobilizing plasmid for re-transfer to occur.
D000150:Acinetobacter / Q000235:genetics*; D000667:Ampicillin; D002701:Chloramphenicol; D010403:Penicillin Resistance; D010957:Plasmids; D011815:R Factors; D013752:Tetracycline; D014295:Trimethoprim
D002701:Chloramphenicol; D000667:Ampicillin; D014295:Trimethoprim; D013752:Tetracycline