A method for the controlled cleavage of disulfide bonds in proteins in the absence of denaturants.
A simple method was developed for the controlled cleavage of protein disulfide bonds and the simultaneous blockage of the free sulfhydryl groups in the absence of a denaturant. The disulfide bonds of bovine serum albumin were cleaved unsymmetrically at pH 7.0 using 0.1 M sulfite in 0.1 M phosphate buffer and the free sulfhydryl groups formed were sulfonated in an oxidation-reduction cycle using molecular oxygen and 400 microM cupric sulfate as a catalyst. The reaction was affected by cupric ion concentration, sulfite concentration, reaction pH and temperature. The standardized method was successfully used to cleave the disulfide bonds of other proteins pepsin, trypsin, and chymotrypsin. The method is reliable and can be used for achieving progressive cleavage of disulfide bonds in proteins without employing a denaturant.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper; D004220:Disulfides; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009579:Nitrobenzoates / Q000138:chemical synthesis; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D011506:Proteins; D012710:Serum Albumin, Bovine; D013696:Temperature
D004220:Disulfides; D009579:Nitrobenzoates; D011506:Proteins; D012710:Serum Albumin, Bovine; D003300:Copper; C041724:2-nitro-5-thiosulfobenzoic acid