Antiviral activity and its mechanism of guanine 7-N-oxide on DNA and RNA viruses derived from salmonid.
Guanine 7-N-oxide produced by Streptomyces sp. was found to inhibit in vitro the replication of herpes virus (Oncorhynchus masou virus, OMV), rhabdo virus (infectious hematopoietic necrosis virus, IHNV) and a bi-segmented double-strand virus (infectious pancreatic necrosis virus, IPNV) derived from salmonids with IC50 values of about 10 micrograms/ml, 20 micrograms/ml and 32 micrograms/ml, respectively. The agent was not toxic for the host cells (chinook salmon embryo, CHSE-214) at the IC50 concentrations. Labeling of IHNV viral RNA and host cellular DNA and RNA with [3H]uridine and [3H]thymidine during drug treatment showed that guanine 7-N-oxide did not reduce the incorporation of these precusors into RNA and DNA. The anti-IHNV activity of guanine 7-N-oxide was enhanced synergistically by neplanocin A, an inhibitor of RNA methylation. The mechanism of action of guanine 7-N-oxide is discussed, in regard to maturation of viral messenger RNA including capping.
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D006147:Guanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006564:Herpesviridae / Q000187:drug effects*; D009696:Nucleic Acids / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D012209:Rhabdoviridae / Q000187:drug effects*; D012474:Salmon / Q000382:microbiology
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D009696:Nucleic Acids; D012367:RNA, Viral; C046075:guanine 7-oxide; D006147:Guanine; C031096:neplanocin A; D000241:Adenosine