Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein.
The variant surface glycoprotein of the parasite Trypanosoma brucei contains a glycolipid of unknown structure covalently attached to its COOH terminus. We have shown, by using metabolic labeling with [35S]methionine or [3H]myristic acid, precipitation with specific antibodies, and NaDodSO4/polyacrylamide gel electrophoresis, that this glycolipid is attached to the variant surface glycoprotein polypeptide within 1 min after its translation.
D000595:Amino Acid Sequence; D000818:Animals; D006017:Glycolipids / Q000378:metabolism*; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D014346:Trypanosoma brucei brucei / Q000276:immunology / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology; D014643:Variant Surface Glycoproteins, Trypanosoma
D006017:Glycolipids; D006023:Glycoproteins; D008565:Membrane Proteins; D009227:Myristic Acids; D014643:Variant Surface Glycoproteins, Trypanosoma; D019814:Myristic Acid; D014415:Tunicamycin