Molecular cloning of a gene sequence regulated by nerve growth factor.
Nerve growth factor (NGF) is essential for the development and differentiation of sympathetic or sensory neurons. A complementary DNA was cloned that corresponds to a gene sequence induced more than 50-fold in a cultured target cell line of pheochromocytoma cells (PC12 cells) 5 hours after the addition of NGF. The induced messenger RNA encodes a 90,000-dalton polypeptide that may represent one of the primary events in NGF-induced differentiation of neurons.
D000199:Actins / Q000235:genetics; D000310:Adrenal Gland Neoplasms / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D009414:Nerve Growth Factors / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D010673:Pheochromocytoma / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats
D000199:Actins; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D004247:DNA