Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors.
A colorimetric assay for peroxidase has been applied to the detection of eosinophils in bone marrow cultures and tissue cell suspensions. The substrate solution consists of 0.1 mM o-phenylenediamine in 0.05 M Tris-HCl buffer pH 8.0 containing 0.1% Triton and 1 mM hydrogen peroxide. The method is shown to be an easy and reproducible method of detecting eosinophils, with insignificant interference from neutrophils.
D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D004804:Eosinophils / Q000166:cytology / Q000201:enzymology; D005122:Exudates and Transudates / Q000166:cytology; D007958:Leukocyte Count; D008645:Mesocestoides; D051379:Mice / Q000469:parasitology; D009504:Neutrophils / Q000201:enzymology; D010544:Peroxidases / Q000097:blood*
D010544:Peroxidases; D000640:Amitrole