Age related variations of some polymorphonuclear leukocyte functions.
The age-related alterations of some biochemical processes in polymorphonuclear leukocytes (PMNL) obtained from 20 healthy aged male and 20 healthy aged female (age: 60-94 years) subjects were investigated. The basic level of luminol dependent chemiluminescence (LDCL) was increased, whereas the basal value of reduced/oxidized glutathione (GSH per GS-SG) was decreased in the aged groups. The enhancement of LDCL was more significant by PMNLs of aged males than of females. The change of both GSH and GS-SG levels during phagocytosis were diminished in the PMNLs of aged subjects. The L-alanine beta-naphthylamide specific elastase like protease (ELP) activity measured in living cell suspension was markedly increased with aging in male subjects. Therefore, it was concluded that the aging of PMNLs is partly a sex related process. The intensive release of both beta-glucuronidase (beta-G) and ELP by the PMNLs of aged subjects after in vitro treatment with calcium ionophore A23187, Cytochalasin B or low density lipoprotein (LDL) suggests that they could play an important role in some age-related disorders.
D000328:Adult; D000368:Aged; D000375:Aging; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D010084:Oxidation-Reduction; D010196:Pancreatic Elastase / Q000378:metabolism; D012737:Sex Factors; D013268:Stimulation, Chemical
D005966:Glucuronidase; D010196:Pancreatic Elastase; D005978:Glutathione