1 alpha,25-Dihydroxyvitamin D3 (calcitriol) stimulates proliferation of human circulating monocytes in vitro.
Previous studies demonstrated that human circulating monocytes can proliferate in vitro when incubated with lectin-induced factor(s) from lymphocytes [(1985) Biochem. Biophys. Res. Commun., in press]. This study shows that human monocytes were induced to proliferate when incubated with 1 alpha,25-dihydroxyvitamin D3 (calcitriol) at physiological concentrations. The optimal dose was about 10 nM. Proliferative activity was examined both by measuring the [3H]thymidine incorporation and by counting cell nuclei. Among other derivatives of vitamin D3, 1 alpha,24R-dihydroxyvitamin D3 and 1 alpha,24R,25-trihydroxyvitamin D3 stimulated mitotic activity of monocytes. Addition of both calcitriol and lectin-stimulated lymphocyte-conditioned medium to the monocyte culture had an additional effect on the mitotic activity of monocytes.
D002117:Calcitriol / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004100:Dihydroxycholecalciferols / Q000494:pharmacology; D006801:Humans; D006887:Hydroxycholecalciferols / Q000494:pharmacology; D007700:Kinetics; D008264:Macrophages / Q000166:cytology; D008938:Mitosis / Q000187:drug effects; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D010835:Phytohemagglutinins; C013721:1,24,25-trihydroxyvitamin D3; C023850:1 alpha,24-dihydroxyvitamin D3; D002117:Calcitriol