Synchronization of rapid globin expression in murine erythroleukemic cells.
The addition of butyric acid (BA) to murine erythroleukemia cells (MELC) produces the expression of primarily A and E2 hemoglobins while DMSO incubation produces the expression of primarily A hemoglobin. Preincubation of MELC with DMSO followed by BA induction accelerates the expression of hemoglobins in a synchronous manner, yielding the preferential synthesis of alpha and y globins, and E2 hemoglobin.
D000818:Animals; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005914:Globins / Q000096:biosynthesis*; D006454:Hemoglobins / Q000096:biosynthesis; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice
D006454:Hemoglobins; D005914:Globins; D004121:Dimethyl Sulfoxide