A rapid, inexpensive, quantitative, general-purpose densitometer and its application to one-dimensional gel electrophoretograms.
We describe the construction of a densitometer based on a TV camera linked to an IBM personal computer (PC) using commercially available components. We discuss some of the problems of accurate quantitative densitometry using low-cost video systems, and describe the manner in which absolute optical density values may be recorded. We describe a digital analysis routine designed to quantitate unresolved bands on noisy one-dimensional electrophoretograms.
D003365:Costs and Cost Analysis; D003720:Densitometry / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation*; D008433:Mathematics; D008838:Microcomputers; D012984:Software; D013690:Television