Role of renal prostaglandins in the fall in urine osmolality after papillary micropuncture.
The effect of micropuncture of the renal papilla through an intact ureter on urinary concentrating ability of rats was examined. Micropuncture of the renal papilla caused a fall in urine osmolality in the punctured kidney from 1718 +/- 106 to 1035 +/- 79 mosmol/kg X H2O. In order to investigate the role of renal prostaglandins in this process, PGE2 excretion was measured and found to increase from 63.4 +/- 14.0 to 205.5 +/- 57.1 pg/min. Urine osmolality and PGE2 excretion from the contralateral kidney were not significantly altered. In animals given meclofenamate (2 mg/kg X hr), renal PGE2 excretion was reduced to 22.3 +/- 5.1 pg/min prior to micropuncture and it remained low at 8.9 +/- 1.8 pg/min after papillary micropuncture. Meclofenamate also blocked the fall in urine osmolality caused by micropuncture of the renal papilla, with urine osmolality averaging 1940 +/- 122 before and 1782 +/- 96 mosmol/kg X H2O after the micropuncture. These results indicated that papillary micropuncture through an intact ureter increased renal PGE2 excretion and that a rise in renal production of PGE2 or some other prostanoid is associated with a fall in urine concentrating ability.
D000818:Animals; D015232:Dinoprostone; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007671:Kidney Concentrating Ability / Q000187:drug effects; D007679:Kidney Medulla; D008469:Meclofenamic Acid / Q000494:pharmacology; D009994:Osmolar Concentration; D011453:Prostaglandins / Q000378:metabolism*; D011458:Prostaglandins E / Q000652:urine; D011677:Punctures; D051381:Rats; D011919:Rats, Inbred Strains
D011453:Prostaglandins; D011458:Prostaglandins E; D008469:Meclofenamic Acid; D015232:Dinoprostone