Purification of glandular kallikrein in maxillary mucosa from humans suffering from chronic inflammation.
Glandular kallikrein was purified from human maxillary mucosa with chronic inflammation and its biochemical properties were characterized. The purification procedure consisted of extraction with 3 mol/l KCl, saturation of ammonium sulfate (66%), DEAE-Sepharose, arginine-Sepharose and Sephadex G-150 chromatographies. Maxillary mucosa with chronic inflammation contains considerable activity of glandular kallikrein, which is a serine protease with limited proteolytic activity and its biochemical properties resemble those of pancreatic kallikrein.
D000328:Adult; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D007610:Kallikreins / Q000302:isolation & purification*; D008437:Maxilla / Q000201:enzymology*; D009092:Mucous Membrane / Q000201:enzymology; D012852:Sinusitis / Q000201:enzymology*; D013696:Temperature; D014361:Trypsin Inhibitors / Q000494:pharmacology
D014361:Trypsin Inhibitors; D007610:Kallikreins