Effect of model trauma on the turnover of protein and hemoprotein components of hepatic microsomal membrane in immature rats.
Administration of 14C-leucine and delta-[3,5-3H]-aminolevulinic acid to immature male rats leads to the incorporation of radioactivity into microsomal protein, including the hemoprotein cytochrome P-450. Non-hepatic regional ischemic trauma results in an increase in the half-life of total microsomal protein, but does not exert the same effect on microsomal heme-associated protein. Loss of radioactivity from microsomal hemoprotein, primarily cytochrome P-450, from traumatized animals exhibits a biphasic pattern similar to that in control animals. The half-life of both the fast-phase component and the slow-phase component is unchanged by trauma. Trauma does, however, increase the ratio of the fast- to slow-phase components of microsomal heme. A significant increase in heme oxygenase activity after trauma suggests that the fast-phase component of hepatic microsomal cytochrome P-450 is more extensively degraded.
D000818:Animals; D000833:Animals, Suckling; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006207:Half-Life; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D006420:Hemeproteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014947:Wounds and Injuries / Q000378:metabolism*
D006420:Hemeproteins; D008054:Lipid Peroxides; D011506:Proteins; D003577:Cytochrome P-450 Enzyme System; D006419:Heme Oxygenase (Decyclizing)