A quantitative comparison of the efficiency of orthograde axonal transport and transsynaptic transport of iodinated (125I) wheat germ agglutinin (I-WGA) and horseradish peroxidase labeled I-WGA (I-WGA-HRP) in the rat visual system.
A quantitative study of the orthograde axonal transport of iodinated wheat germ agglutinin (I-WGA) and I-WGA conjugated to horseradish peroxidase (I-WGA-HRP) demonstrated labeling of both the lateral geniculate nucleus and the visual cortex following intraocular injections. I-WGA was 13 times more efficient than I-WGA-HRP as a marker of the primary projection from the retina to the geniculate and 3 times more efficient than I-WGA-HRP as a marker of the second order projection to the cortex.
D000818:Animals; D001345:Autoradiography; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport; D002850:Chromatography, Gel; D004563:Electrochemistry; D006735:Horseradish Peroxidase; D037102:Lectins; D010544:Peroxidases; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*; D013787:Thalamic Nuclei / Q000378:metabolism; D014793:Visual Cortex / Q000378:metabolism; D014795:Visual Pathways / Q000378:metabolism*; D014909:Wheat Germ Agglutinins
D037102:Lectins; D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase; D010544:Peroxidases