Molecular forms of glucagon-like peptides in man.
Molecular forms of the glucagon-like peptides (GLP) encoded by the human preproglucagon gene were analysed by chromatography combined with specific radioimmunoassays to the synthetic peptides. Whereas extracts of human pancreas and a glucagonoma contained a large proglucagon cleavage product possessing both GLP-1 and GLP-2 immunoreactivities, extracts of human intestine contained products corresponding to free GLP-1 and a small amount of chromatographically distinct GLP-2 immunoreactivity. It is concluded that post-translational processing of proglucagon differs in pancreas and intestine, so that the C-terminal portion of the molecule is cleaved to liberate free GLP-1 in the intestine. Further processing or degradation results in loss especially of GLP-2 immunoreactivity.
D002851:Chromatography, High Pressure Liquid; D005796:Genes; D052216:Glucagon-Like Peptide 1; D053767:Glucagon-Like Peptide 2; D005935:Glucagonoma / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism; D010455:Peptides / Q000235:genetics* / Q000302:isolation & purification; D011498:Protein Precursors / Q000235:genetics*; D011863:Radioimmunoassay
D053767:Glucagon-Like Peptide 2; D010455:Peptides; D011498:Protein Precursors; D052216:Glucagon-Like Peptide 1