Flow-injection analysis of catecholamine secretion from bovine adrenal medulla cells on microbeads.
Bovine adrenal medullary cells have been cultured on microbeads which are placed in a low-volume flow system for measurements of stimulation-response parameters. Electronically controlled stream switching allows stimulation of cells with pulse lengths from 1 s to many minutes; pulses may be repeated indefinitely. Catecholamines secreted are detected by an electrochemical detector downstream from the cells. This flow-injection analysis technique provides a new level of sensitivity and precision for measurement of kinetic parameters of secretion. A manual injection valve allows stimulation by higher levels of stimulant in the presence of constant low levels of stimulant. Such experiments show interesting differences between the effects of K+ and acetylcholine on cells partially desensitized to acetylcholine.
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D001547:Benzaldehydes / Q000138:chemical synthesis*; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D005898:Glass; D007700:Kinetics; D008863:Microspheres
D001547:Benzaldehydes; D002395:Catecholamines; D005456:Fluorescent Dyes; C044970:2-benzoylbenzaldehyde; C044969:2-acetylbenzaldehyde