Characterization of a glycoprotein fraction with an unusual composition from eel serum.
A glycoprotein fraction was extracted from eel serum with a high carbohydrate content of 60% as determined by gas-liquid chromatography. Remarkable is the presence of glucose (5%) which is not attributable to a contaminating glycolipid. Affinity chromatography on wheat germ agglutinin immobilized on to agarose beads results in two glycoproteins with a molecular weight of 17200 and 31300, respectively, as determined by SDS-PAGE (polyacrylamide gel electrophoresis). They differ markedly in their sialic acid content. All of the neuraminic acid is terminally bound and exclusively N-acetylated.
D000811:Anguilla / Q000097:blood*; D000818:Animals; D002846:Chromatography, Affinity; D002849:Chromatography, Gas; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000097:blood*; D037102:Lectins; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D012794:Sialic Acids; D014909:Wheat Germ Agglutinins; D019158:N-Acetylneuraminic Acid; D005947:Glucose