Controlled binding of liposomes to cultured cells by means of lectins.
Lectin-mediated binding of liposomes to Hela cells was analyzed as a function of different parameters. We show that the amount of lectin covalently bound to liposomes can be accurately controlled. We chose to work with 500 - to 1 000 molecules of WGA bound per liposome of 1 micron diameter. These liposomes bound very efficiently to Hela cells as demonstrated by fluorescent microscopy, and fluorescent cell-sorting. We show that the number of liposomes bound is proportional to the input, over a wide range of concentrations. The liposomes bound very tightly to cells and could not be removed by trypsin or N-acetylglucosamine, which competes with WGA binding.
D000117:Acetylglucosamine / Q000378:metabolism; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism*; D002469:Cell Separation; D005434:Flow Cytometry; D005452:Fluoresceins / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010835:Phytohemagglutinins / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism; D014909:Wheat Germ Agglutinins
D005452:Fluoresceins; D037102:Lectins; D008081:Liposomes; D010835:Phytohemagglutinins; D014909:Wheat Germ Agglutinins; D014357:Trypsin; C007740:fluorexon; D000117:Acetylglucosamine