Independent action of prostaglandins and kinins on vasopressin-stimulated water flow.
The kallikrein-kinin and the prostaglandin systems are both important modifiers of vasopressin action. This study examines whether the systems are dependent on one another for their action. Four groups of toad hemibladders were examined. In groups 1 and 2 animals the endogenous prostaglandin system was inhibited. Inhibition of kallikrein by aprotinin caused vasopressin-stimulated water flow to increase further (24.8 +/- 4.9 to 34.5 +/- 4.8 microliters/min) while potentiation of kinins by captropril caused vasopressin-stimulated water flow to decrease (45 +/- 6.3 to 30.5 +/- 5.4 microliters/min). In groups 3 and 4 endogenous kallikrein was inhibited by aprotinin. The addition of prostaglandin E2 caused vasopressin-stimulated water flow to decrease (17.5 +/- 2.7 to 5.71 +/- 1.0 microliter/min) while the inhibition of endogenous prostaglandins caused vasopressin-stimulated water flow to increase (26.7 +/- 3.4 to 39.2 +/- 3.5 microliters/min). Thus, the inhibitory effects of prostaglandins and kinins on vasopressin-stimulated water flow are independent of one another.
D000818:Animals; D001001:Anura; D001059:Apoproteins / Q000494:pharmacology; D001834:Body Water / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007610:Kallikreins / Q000502:physiology; D007705:Kinins / Q000502:physiology*; D009995:Osmosis / Q000187:drug effects; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D011453:Prostaglandins / Q000502:physiology*; D001743:Urinary Bladder / Q000378:metabolism; D014667:Vasopressins / Q000494:pharmacology*
D001059:Apoproteins; D007705:Kinins; D011448:Prostaglandin Antagonists; D011453:Prostaglandins; D014667:Vasopressins; D007610:Kallikreins