Isolation of tumor-secreted products from human carcinoma cells maintained in a defined protein-free medium.
A protein-free synthetic cell-growth medium has been defined that permits long-term survival (greater than 120 days) of an established human colon tumor cell line, HT-29. Viability is dependent upon both the concentration of L-glutamine in the medium and the cell density at the time of initial transfer into it. Cell proliferation is minimal, thus obviating the necessity for subculturing. HT-29 adenocarcinoma cells maintained in large-scale culture with this medium continue to secrete the established colon tumor marker carcinoembryonic antigen as well as growth factors and lysozyme. These and, potentially, other important tumor-derived products can therefore be generated continuously in such cultures so that they can be isolated from a conditioned medium free of contaminating serum and protein supplements.
D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D003470:Culture Media; D005354:Fibrosarcoma / Q000378:metabolism / Q000473:pathology; D005973:Glutamine; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D009113:Muramidase / Q000378:metabolism; D011506:Proteins
D002272:Carcinoembryonic Antigen; D003470:Culture Media; D006133:Growth Substances; D011506:Proteins; D005973:Glutamine; D009113:Muramidase