ACNU-resistant mutants of 9L rat glioma cell line. Isolation and preliminary characterization of these subclones.
Three ACNU-resistant subclones were isolated and characterized from a wild-typed 9L rat glioma cell line in culture. At an early stage after cloning, these ACNU-resistant subclones showed a high frequency of chromosomal aberrations compared with nonresistant 9L cells. These ACNU-resistant subclones revealed a cross resistance to BCNU, CCNU, methyl CCNU, nitrogen mustard, cyclophosphamide, and cis-platinum, which are alkylating agents. Further studies are necessary to clarify the mechanisms of ACNU-resistance from the aspect of repair of DNA alkylation damage.
D000818:Animals; D001932:Brain Neoplasms / Q000188:drug therapy*; D002460:Cell Line; D004351:Drug Resistance; D005910:Glioma / Q000188:drug therapy*; D009374:Neoplasms, Experimental / Q000188:drug therapy; D015376:Nimustine; D009607:Nitrosourea Compounds / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344
D009607:Nitrosourea Compounds; D015376:Nimustine