Isolation and characterization of a cytolysin produced by Vibrio cholerae serogroup non-O1.
A thermolabile toxin (molecular weight, 52 711; isoelectric point, 8.65) produced by a clinical isolate of Vibrio cholerae serogroup non-O1 was cytotoxic for Y-1 mouse adrenal cells and Chinese hamster ovary cells. The toxin lysed rabbit red blood cells and produced a hemorrhagic zone in rabbit skin. When injected intravenously into adult mice, the cytolysin was rapidly lethal and caused fluid accumulation in both 5- and 18-h rabbit ileal loops. Strains of V. cholerae that produced cytolysin but no cholerae enterotoxin were able to cause fluid accumulation in rabbit intestinal loops.
D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology; D002460:Cell Line; D002470:Cell Survival; D002850:Chromatography, Gel; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins / Q000276:immunology / Q000302:isolation & purification* / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006461:Hemolysis; D007082:Ileum; D007106:Immune Sera; D007413:Intestinal Mucosa / Q000378:metabolism; D007526:Isoelectric Point; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009500:Neutralization Tests; D011817:Rabbits; D022603:Shiga Toxins; D013997:Time Factors; D014734:Vibrio cholerae / Q000378:metabolism*
D001427:Bacterial Toxins; D003603:Cytotoxins; D007106:Immune Sera; D022603:Shiga Toxins