High long-standing fertilizing capacity of human sperm isolated for male sex preselection.
A method of separating a Y-enriched human sperm population for male sex preselection with a single-column, four-step, four-layer albumin gradient technique is presented. This technique provides an efficient separation of spermatozoa, good Y-enriched sperm motility, and high fertilization capacity of this fraction as tested by the zona-free hamster ova sperm penetration assay.
D002469:Cell Separation / Q000379:methods; D005260:Female; D005818:Genetic Engineering; D006801:Humans; D008297:Male; D012732:Sex Determination Analysis; D012743:Sex Preselection; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors; D014998:Y Chromosome / Q000648:ultrastructure