A new approach to gene mapping by in situ hybridization on isolated chromosomes.
A new technique was developed for in situ hybridization on isolated murine chromosomes. The safety and relative rapidity of the method is due to the ready availability of large numbers of isolated "target" chromosomes with well preserved morphology. Its applicability was demonstrated by mapping c-myc to band 15D of Robertsonian (6;15) fusion chromosomes. This localization coincides with the cytogenetic mapping of the translocation breakpoints in mouse plasmacytomas that carry the typical rcpt (12;15) translocation.
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002874:Chromosome Mapping; D005819:Genetic Markers; D007621:Karyotyping; D008223:Lymphoma / Q000235:genetics; D051379:Mice; D008806:Mice, Inbred AKR; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D013953:Thymus Neoplasms / Q000235:genetics; D014178:Translocation, Genetic
D005819:Genetic Markers