Avian skin embryonal fibroblasts heterogeneity for lectins surface receptors.
Using several fluorescein-coupled lectins (ConA, WGA and SBA) the distribution of surface ligands in chick embryonic skin fibroblasts was studied at two incubation stages. On the basis of the percentage of lectin marked cells, at least, three fibroblastic populations heterogeneous for surface specific-saccharide binding sites were found. Their relative concentration were changed in the course of incubation, thus indicating developmental changes. We discuss this finding in relation to the regulatory mechanism of the spatial and temporal mesenchymal glycosaminoglycan pattern.
D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D003208:Concanavalin A; D005347:Fibroblasts / Q000166:cytology; D037102:Lectins; D008856:Microscopy, Fluorescence; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis*; D012867:Skin / Q000166:cytology* / Q000196:embryology; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; D010835:Phytohemagglutinins
D003208:Concanavalin A; D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C028824:soybean lectin; D010835:Phytohemagglutinins