Efficient generation of antibodies to oncoproteins by using synthetic peptide antigens.
To examine the efficiency of generating protein-reactive antipeptide antibodies, 35 peptides encoded by retroviral or cellular oncogenes were used to immunize rabbits. Thirty-two peptides elicited antipeptide antibodies, of which 56% reacted with their respective oncoproteins. The length of the immunizing peptide was an important factor in generating antibodies reactive with native protein. Similar peptides differing in a single or a few amino acids could elicite antisera of markedly different reactivities.
D000595:Amino Acid Sequence; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D009363:Neoplasm Proteins / Q000276:immunology*; D009857:Oncogenes; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011487:Protein Conformation; D012995:Solubility; D013329:Structure-Activity Relationship
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D010446:Peptide Fragments