Isolation of methyl 3-hydroxy-9-oxo-9,10-seco-23,24-dinor-1,3,5(10)-cholatrienoate from a sterol bioconversion.
A mutant (UC9778) of M. fortuitum has been isolated which degrades cholesterol and plant sterols to mixtures of Ring A-phenolic compounds. The title compound was isolated by repeated chromatography, and its structure determined by spectroscopic methods.
D002784:Cholesterol / Q000378:metabolism; D005285:Fermentation; D013058:Mass Spectrometry; D009154:Mutation; D009161:Mycobacterium / Q000378:metabolism*; D011281:Pregnatrienes / Q000302:isolation & purification* / Q000378:metabolism; D012632:Secosteroids / Q000302:isolation & purification* / Q000378:metabolism; D013261:Sterols / Q000378:metabolism
D011281:Pregnatrienes; D012632:Secosteroids; D013261:Sterols; C048869:methyl 3-hydroxy-9-oxo-9,10-seco-23,24-dinor-1,3,5(10)-cholatrienoate; D002784:Cholesterol