6-Oxo-prostaglandin F1a production by guinea-pig skeletal muscle in vitro: the effects of oestradiol and progesterone.
Fragments of guinea-pig skeletal muscle produce four times as much 6-oxo-PGF1a as PGE and PGF. This production of 6-oxo-PGF1a was unaffected by relatively high concentrations of oestradiol-17B (100 micrograms/ml) but was increased four-fold in the presence of progesterone (500 micrograms/ml). In skeletal muscle homogenates, oestradiol (100 micrograms/ml) inhibited 6-oxo-PGF1a synthesis and this inhibition was enhanced in the presence of progesterone (500 micrograms/ml); at this concentration, progesterone had no effect on its own. Tamoxifen (100 micrograms/ml), a drug with anti-oestrogenic properties in humans, enhanced the inhibition of 6-oxo-PGF1a synthesis by oestradiol and by oestradiol and progesterone together, suggesting that oestradiol and tamoxifen may exert their effect by a similar mechanism in this experimental model. The significance of these observations is discussed.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D046508:Culture Techniques; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D006168:Guinea Pigs; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D013629:Tamoxifen / Q000494:pharmacology
D004965:Estrogen Antagonists; D011458:Prostaglandins E; D011460:Prostaglandins F; D013629:Tamoxifen; D011374:Progesterone; D004958:Estradiol; D015121:6-Ketoprostaglandin F1 alpha