The influence of sorbitol on the glycogenolytic pathway.
The following report presents the results of a study conducted on the influence of sorbitol on levels of glycogen, phosphorylase a (E.C. 2.4.1.1.) and phosphorylase kinase (E.C.2.7.1.38) in slices of rat liver. In addition, it presents data concerning the effects of sorbitol on the activities of phosphorylase a and phosphorylase kinase purified from rabbit muscle. The data reveal a limitation in the glycogenolytic process which seems, in part, due to the inactivation of the phosphorylase a and phosphorylase kinase. Gel-filtration techniques showed, in fact, a breakdown of these two oligomeric enzymes into inactive subunits following incubation with sorbitol.
D000818:Animals; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008112:Liver Glycogen / Q000378:metabolism*; D008297:Male; D010764:Phosphorylase Kinase / Q000378:metabolism*; D010762:Phosphorylase a / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013012:Sorbitol / Q000494:pharmacology*
D008112:Liver Glycogen; D013012:Sorbitol; D010762:Phosphorylase a; D006005:Phosphorylases; D010764:Phosphorylase Kinase