Construction and identification of a cDNA clone for human type II procollagen mRNA.
Double-stranded cDNA was constructed for poly(A)-containing RNA isolated from foetal human articular cartilage known to contain small amounts of pro alpha 1 (II) collagen mRNA. A 585 base pair PstI-EcoRI cDNA fragment was isolated and cloned into plasmid pBR322. A resulting recombinant plasmid pHCAR1 was shown to hybridize specifically to a 5.4 kilobase mRNA in cartilage but not in calvarial RNA. Definite identification of clone pHCAR1 was based on sequence analysis; marked homology with the corresponding chick gene and complete agreement with the human gene sequences available were observed.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004587:Electrophoresis, Agar Gel; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011061:Poly A; D011347:Procollagen; D012333:RNA, Messenger; D014018:Tissue Distribution
D011347:Procollagen; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA