Serotonin-depleting midbrain lesions fail to mitigate hyperphagia and obesity in the Zucker fatty rat.
Previous research has shown that damage to the dorsal and median raphe nuclei of rats can impede the subsequent development of hypothalamic hyperphagia and obesity as well as impair the defense of established hypothalamic obesity in response to food deprivation. The present study sought to determine if raphe injury might alter the development of another form of obesity, namely that which occurs spontaneously in the Zucker fatty rat. Subjects were 20 obese females (fafa; mean weight of 200 g) and 20 lean littermate controls (FaFa females; mean weight of 150 g). Following 10 days of baseline intake and weight recordings, half of each group received radio-frequency heat lesions of the dorsal and median raphe nuclei while the other half received sham surgery. Except for a mild suppression of food intake and weight gain during the first few days after lesioning, raphe injury did not alter the hyperphagia or obesity shown by fatties over the 7 week ad lib feeding period studied. Additional 24-hr intake tests of varying sucrose and quinine solutions revealed reduced sucrose acceptance and enhanced quinine rejection by fatties much as has been seen in previous studies of hypothalamic obese rats. Terminal assays of forebrain monoamine levels confirmed that raphe lesions were effective in depleting serotonin (-71% compared to controls) without producing major changes in norepinephrine or dopamine (-14% and +2%, respectively). The inability of raphe lesions to mitigate this form of hyperphagia and obesity suggests that earlier observations of their attenuating effects on hypothalamic obesity were not due to non-specific impairments of behavioral or metabolic factors necessary to permit overeating and weight gain.
D000818:Animals; D001835:Body Weight; D001931:Brain Mapping; D001068:Feeding and Eating Disorders / Q000503:physiopathology*; D005260:Female; D006801:Humans; D006963:Hyperphagia / Q000503:physiopathology*; D008636:Mesencephalon / Q000503:physiopathology*; D009765:Obesity / Q000503:physiopathology*; D010641:Phenotype; D011903:Raphe Nuclei / Q000503:physiopathology*; D051381:Rats; D011924:Rats, Zucker; D012701:Serotonin / Q000502:physiology*
D012701:Serotonin