Identification and measurement of paraprotein polymers by high performance gel filtration chromatography.
An evaluation of Superose 6, a new high performance gel filtration medium, has been made for the rapid separation and quantitation of paraprotein polymers. There is a significant correlation between relative serum viscosity and the concentration of polymeric IgA as demonstrated by FPLC using the Superose 6 columns. The retention times of the columns are highly reproducible and allow good resolution of polymers and provide a simple way of separating IgG3 from albumin. Scaling up to the preparative Superose 6B could be achieved with little loss of resolution.
D002850:Chromatography, Gel / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D010266:Paraproteins / Q000032:analysis* / Q000302:isolation & purification; D011108:Polymers / Q000032:analysis; D012685:Sepharose
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010266:Paraproteins; D011108:Polymers; C043127:Superose 6b; D012685:Sepharose