Enzymatic characterization of arginine esterase from dog seminal plasma.
Previously purified arginine esterase from dog seminal plasma was characterized enzymatically. The enzyme was found to have a rather narrow specificity for arginine esters, much less for lysine esters and was practically devoid of activity towards tyrosine esters, casein, albumin and azocoll. It had a broad optimum pH between 8 and 9. It presented no kallikrein-like activities either in the blood pressure test in dog or in the rat uterus contraction test. It was inhibited by bovine pancreas trypsin inhibitor, aprotinin, phenylalanylprolyl arginine chloromethyl ketone, diisopropylfluorophosphate, phenylmethylsulfonyl fluoride, sodium dodecyl sulfate and leupeptin, but not by soybean trypsin inhibitor, tosyllysine chloromethyl ketone, tosylamide-2-phenylethyl chloromethyl ketone, iodoacetamide, Triton X-100 and EDTA. Experiments involving incubation of prostatic cytosol with purified arginine esterase showed that actin was the only important prostatic protein that was extensively hydrolyzed by this enzyme. It is not known presently whether the hydrolysis of actin is related to a true physiological function of the enzyme and whether actin and arginine esterase ever come into contact with each other in vivo. These properties indicate that arginine esterase from dog seminal plasma is different from other known proteinases including classical kallikreins, although it presents many similarities with this class of enzyme.
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004285:Dogs; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007610:Kallikreins / Q000378:metabolism; D007700:Kinetics; D008297:Male; D012661:Semen / Q000201:enzymology*; D014357:Trypsin / Q000378:metabolism; D014508:Urea / Q000494:pharmacology
D014508:Urea; D001120:Arginine; C009727:benzoylarginine ethyl ester; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D007610:Kallikreins; D014357:Trypsin