[Binding of activated C3b component with complement effector].
Various nucleophilic agents (acceptors) react with thiolester group of nascent activated fragment (C3b) of the third complement component. The C3b-acceptors binding prevents transformation of C3 convertase to C5 convertase and results in inhibition of the cell-target lysis. A convenient method of monitoring the EAC142 to EAC1423 transformation was elaborated. Character of the inhibition suggests that the covalent binding follows a stage of the reversible C3b-acceptor complex formation. The method allows to determine the maximum of inhibition of the C5 convertase formation and the dissociation constant of the reversible C3b-acceptor complex, which reflects the C3b affinity to this acceptor.
D000818:Animals; D002021:Buffers; D003166:Complement Activating Enzymes / Q000037:antagonists & inhibitors*; D050577:Complement C3-C5 Convertases / Q000037:antagonists & inhibitors*; D003179:Complement C3b / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D006461:Hemolysis; D066298:In Vitro Techniques; D007700:Kinetics; D011951:Receptors, Complement / Q000378:metabolism*; D012756:Sheep
D002021:Buffers; D011951:Receptors, Complement; D003179:Complement C3b; D003166:Complement Activating Enzymes; D050577:Complement C3-C5 Convertases
Sviazyvanie aktivirovannogo komponenta C3b s éffektorami komplementa.