Biosynthesis of androgens and pheromonal steroids in neonatal porcine testicular preparations.
The biosynthesis of testosterone and 4-androstene-3,17-dione and some 16-androstenes has been studied in homogenates or subcellular fractions of testes from 3-week-old Landrace piglets. Pregnenolone was converted into 5,16-androstadien-3 beta-ol, 4,16-androstadien-3-one, 5 alpha-androst-16-en-3-one and 5 alpha-androst-16-en-3 alpha- and 3 beta-ols, but the quantities were some 50 times less than those formed in the mature boar testis. Androgens were also formed in the microsomal fractions but the quantities of 4-androstene-3,17-dione (from side-chain cleavage of 17-hydroxyprogesterone) and of testosterone (from reduction of 4-androstene-3,17-dione) were 50-70 times lower than in the adult animal. The kinetic parameters and cofactor preference of the 3 alpha- and 3 beta-hydroxysteroid dehydrogenases were determined in the cytosolic, microsomal and mitochondrial fractions of neonatal porcine testes.
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000728:Androgens / Q000096:biosynthesis*; D000736:Androstenes / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010675:Pheromones / Q000096:biosynthesis*; D011284:Pregnenolone / Q000378:metabolism; D013552:Swine; D013737:Testis / Q000378:metabolism*
D000728:Androgens; D000736:Androstenes; D010675:Pheromones; D011284:Pregnenolone; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases