Location of functional regions of acetylcholine receptor alpha-subunit by site-directed mutagenesis.
The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with alpha-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the alpha-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002217:Carbachol / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D005786:Gene Expression Regulation; D046911:Macromolecular Substances; D008564:Membrane Potentials / Q000187:drug effects; D009154:Mutation; D012333:RNA, Messenger / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000235:genetics*; D014101:Torpedo; D014981:Xenopus
D046911:Macromolecular Substances; D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D002217:Carbachol; D004247:DNA