Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA.
The positions of intramolecular crosslinks induced by irradiation with ultraviolet light were mapped into potato spindle tuber viroid RNA and HeLa 5S rRNA. Crosslinking in each of these molecules occurred at a single major site, which was located by RNA fingerprinting and secondary analysis (and additional primer extension studies in the case of the viroid). Various lines of evidence suggest that these crosslinks identify a previously undescribed element of local tertiary structure common to these two widely divergent RNA molecules: (i) both crosslinks occur in an identical eight-base context, with the sequence 5' GGGAA 3' on one side and the sequence 5' UAC 3' on the other; (ii) both crosslinks connect bases that are not thought to be involved in conventional hydrogen bonding, within regions usually depicted as single-stranded loops flanked by short helical segments; and (iii) both crosslinks connect a purine and a pyrimidine residue, and both may generate the same G-U dimer. Furthermore, it is likely that the crosslinking site is of functional significance because it is located within the most highly conserved region of the viroid sequence and involves bases that are essentially invariant among eukaryotic 5S rRNA molecules.
D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis* / Q000528:radiation effects; D012335:RNA, Ribosomal / Q000032:analysis* / Q000528:radiation effects; D012367:RNA, Viral / Q000032:analysis* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014772:Viroids / Q000032:analysis*
D009838:Oligodeoxyribonucleotides; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D012367:RNA, Viral