Ion selective microelectrodes: computer-controlled calibration, plotting, and data analysis.
Calibration of ion-selective microelectrodes involves delivering and measuring the potential of several different solutions both before and after intracellular measurement. A microprocessor-controlled system is described which completely automates that tedious process. The microcomputer controls the delivery of the calibration solutions and prints out the calibration value along with the solution number. A second program plots the calibration curve using a modified Nicholsky equation and a third program converts intracellular ion signals into ion concentration. Specific examples based on Ca-selective microelectrodes are shown.
D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D002118:Calcium / Q000032:analysis; D002138:Calibration; D003201:Computers; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D008838:Microcomputers; D008839:Microelectrodes; D012984:Software
D002118:Calcium