Hormone-independent polyamine metabolism of squamous cell carcinoma of the prostate.
The levels of polyamines and their synthesizing enzymes in squamous cell carcinoma of prostate implanted in intact as well as castrated male rats were determined after certain hormonal manipulations. The tumour was found to grow with an identical rate in non-castrated and castrated rats. Polyamine content and activities of polyamine synthesizing enzymes in the tumour were found to be much lower compared to their values in ventral prostate. Moreover, the levels of these parameters were comparable in tumours whether implanted in non-castrated or gonadectomized animals. The sequential analyses of putrescine and spermidine and activities of L-ornithine decarboxylase and S-adenosyl-L-methionine decarboxylase of tumours at different time intervals showed a significant reduction in their levels at 30 days compared to 10 days post implantation in non-castrated as well as castrated rats. Daily intramuscular administration of tumour-bearing intact or castrated animals with testosterone (50 micrograms/g), beta-estradiol (2 micrograms/g) or cyproterone (12.5 micrograms/g) for 10 days did not influence polyamine metabolism in tumour tissue. However, either beta-estradiol and cyproterone treatments or castration were found to decrease polyamine synthesis in ventral prostate. At the same time, the testosterone replacement therapy did not allow polyamine levels or activities of polyamine synthesizing enzymes to decline in the ventral prostate of castrated rats. Our results demonstrated that contrary to ventral prostate, the polyamine metabolism in squamous cell carcinoma of prostate is independent of hormonal control. The loss of hormonal sensitivity of polyamine metabolism in the prostatic tumour could be the result of qualitative changes that occurred during transformation.
D012437:Adenosylmethionine Decarboxylase / Q000032:analysis; D000728:Androgens / Q000502:physiology; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D002369:Castration; D003534:Cyproterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology; D006728:Hormones / Q000502:physiology*; D008297:Male; D009368:Neoplasm Transplantation; D009955:Ornithine Decarboxylase / Q000032:analysis; D011073:Polyamines / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D011700:Putrescine / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D013739:Testosterone / Q000494:pharmacology
D000728:Androgens; D004967:Estrogens; D006728:Hormones; D011073:Polyamines; D013096:Spermine; D013739:Testosterone; D004958:Estradiol; D003534:Cyproterone; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; D013095:Spermidine; D011700:Putrescine