Correct sequence for the major nucleocapsid protein mRNA of respiratory syncytial virus.
A nucleotide sequence for the mRNA of the major nucleocapsid (N) protein gene of respiratory syncytial virus was reported previously (N. Elango and S. Venkatesen, 1983, Nucleic Acids Res. 11, 5941-5951). However, we have been unable to confirm part of this sequence as N mRNA-specific and suggest that the published sequence represents that of an aberrant chimeric transcript. Here we present an alternative sequence for the N mRNA and provide data supporting its authenticity. The corrected N mRNA sequence contains 1197 rather than 1427 nucleotides exclusive of poly(A), and encodes a protein of 391 rather than 467 amino acids. The calculated molecular weight for the 391-amino acid protein described by the sequence presented here is 42,600, in agreement with the molecular weight of 42,000 determined for the RS viral N protein by gel electrophoresis. In addition, we present sequence data from dicistronic RNAs that span the junction between the 1B protein and N cistrons, and the junction between the N and phosphoprotein (P) cistrons.
D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D004247:DNA; D005796:Genes; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA