[Lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase in skeletal muscle. 12. The influence of electric stimulation of beef carcasses on activity and subcellular distribution].
The effect of electrical stimulation (ES) of beef carcasses at 450 V on the total extractable activity and subcellular distribution of the mitochondrial enzymes lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase in skeletal muscle (activities in the supernatant of a phosphate buffer extract and in muscle press juice) was studied. There was no influence of ES on the total activity and the subcellular distribution of these enzymes in the muscle tissue stored at +2 degrees C for 7 days nor did ES influence the extent of the release of the three enzymes from the mitochondria into the sarcoplasm by freezing (-20 degrees C) and thawing. From these results it can be concluded that ES does not result in an appreciable disintegration of the inner membrane of muscle mitochondria.
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D009132:Muscles / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors
D015094:3-Hydroxyacyl CoA Dehydrogenases; D008058:Dihydrolipoamide Dehydrogenase; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases
Lipoamiddehydrogenase, Citratsynthase und beta-Hydroxyacyl-CoA-dehydrogenase des Skelettmuskels. XII. Einfluss der Elektrostimulierung von Rinderschlachtkörpern auf Aktivität und subcelluläre Verteilung.