Cross-reactivities of neuropeptide Y and peptide YY with pancreatic polypeptide antisera: evidence for the existence of pancreatic polypeptide in the brain.
Highly purified neuropeptide Y (NPY) and peptide YY (PYY) did not cross-react in our human pancreatic polypeptide (hPP) radioimmunoassay, nor did 125I-labelled NPY and PYY, even with anti-hPP serum at low dilution (1:1000). However, both [125I]NPY and [125I]PYY significantly cross-reacted with anti-bovine PP (bPP) serum at low dilution (1:1000, similar to that used in immunohistochemistry). These results suggest that radioassayable hPP-like peptide in the porcine or canine brain is probably pancreatic polypeptide itself, otherwise immunohistochemically detected bPP-like peptide may represent both NPY and PP.
D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds; D001923:Brain Chemistry; D002417:Cattle; D003429:Cross Reactions; D004285:Dogs; D006801:Humans; D009419:Nerve Tissue Proteins / Q000276:immunology*; D009478:Neuropeptide Y; D010191:Pancreatic Polypeptide / Q000032:analysis* / Q000276:immunology; D019894:Peptide YY; D010455:Peptides / Q000276:immunology*; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D010455:Peptides; D019894:Peptide YY; D010191:Pancreatic Polypeptide