[Characteristics of virion formation during mixed infection with influenza viruses A and B].
Simultaneous infection of MDCK cells with influenza A and B viruses at an equal multiplicity of infection leads to the synthesis of the proteins of both viruses. In the population of virions the hemagglutinin of influenza B virus prevails, whereas NP proteins of both viruses are present in similar quantities. Trypsin treatment of the double-infected cells resulting in the cleavage of the hemagglutinin molecules at the cell surface allows revealing the predominance of influenza B hemagglutinin on cell surface, although both hemagglutinins are accumulated in the cells. An impairment of the hemagglutinin transport to the cell surface as a possible additional mechanism of heterotypic interference and its possible effect on the polypeptide content of the phenotypically mixed virions are discussed.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D006389:Hemagglutinins, Viral / Q000096:biosynthesis; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D009981:Influenza B virus / Q000235:genetics* / Q000502:physiology; D051379:Mice; D014762:Viral Interference; D014771:Virion / Q000235:genetics*
D006389:Hemagglutinins, Viral
Osobennosti formirovaniia virionov pri smeshannoĭ infektsii, vyzvannoĭ virusami grippa A i B.