Isolation of a mutant cell line derived from ICR 2A frog cells hypersensitive to the induction of non-dimer DNA damage by solar ultraviolet radiation.
A mutant cell line DRP 36, hypersensitive to nondimer DNA damage induced by exposure of cells to the Mylar-filtered solar ultraviolet (UV) radiation produced by a fluorescent sunlamp plus photoreactivating light (PRL) was isolated from the haploid ICR 2A frog cell line. The DO for mutant cells exposed to this solar UV source was 3.3 kJ/m2 compared with a DO of 7.3 kJ/m2 for the parental ICR 2A cells. In contrast, DRP 36 and ICR 2A cells exhibited similar levels of survival following 254-nm irradiation which causes the induction primarily of pyrimidine dimers. The cross-sensitivity to additional DNA damaging agents was examined, and it was determined that the DRP 36 cells are also hypersensitive to treatment with gamma-rays, ethyl methane sulfonate (EMS), cis-dichlorodiammine platinum (II) (DDP), and 4-nitroquinoline oxide (4-NQO) while exhibiting normal sensitivity to L-phenylalanine mustard (L-PAM), 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) and mitomycin C (MMC).
D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000032:analysis; D011836:Radiation Tolerance; D011898:Ranidae; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D004247:DNA; D004255:Deoxyribodipyrimidine Photo-Lyase