Regional mapping of two human immunoglobulin V lambda genes and analysis of the V lambda locus in chronic myeloid leukaemia.
The human immunoglobulin V lambda locus has been studied in relation to chromosomal translocations involving chromosome 22. DNA probes for two V lambda genes which belong to different subgroups and do not cross hybridize, were used to show that both V lambda genes are located on the Philadelphia chromosome in chronic myeloid leukaemia (CML). Both genes map in band 22q11 to a region that is bounded on the distal side by the breakpoints for CML 9:22 translocations and on the proximal side by the breakpoint for an X:22 translocation. We have found no evidence for rearrangements or amplification of either V lambda gene in CML, in either the chronic or acute phases of the disease. In K562 cells which are derived from the pleural effusion of a patient with Ph1-positive CML, there appears to be no rearrangement of the V lambda genes, but they are both amplified about four times. We have estimated that the minimum size for the amplification unit in K562 cells is 186 kb.
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002904:Chromosomes, Human, 21-22 and Y; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D005796:Genes; D005838:Genotype; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics / Q000276:immunology*; D009693:Nucleic Acid Hybridization; D012016:Reference Values; D014178:Translocation, Genetic
D007135:Immunoglobulin Variable Region; D004247:DNA