A simple, rapid, high yield isolation and purification procedure for chloroperoxidase isoenzymes.
A simple four-step procedure has been developed for isolation of chloroperoxidase from the mold Caldariomyces fumago. Polyethyleneglycol precipitation of the contaminating pigment in the growth medium, followed by chromatography of the soluble enzyme fraction on a QAE-ZetaPrep-250 cartridge and ammonium sulfate precipitation affords isolation of the chloroperoxidase. Extensive dialysis and chromatography on DE-53 cellulose allows the separation and further purification of chloroperoxidase A and B isoenzymes.
D000645:Ammonium Sulfate; D011232:Chemical Precipitation; D002711:Chloride Peroxidase / Q000302:isolation & purification*; D002845:Chromatography; D003956:Dialysis; D004586:Electrophoresis; D007527:Isoenzymes / Q000302:isolation & purification*; D003904:Mitosporic Fungi / Q000201:enzymology*; D010544:Peroxidases / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D013053:Spectrophotometry
D007527:Isoenzymes; D011092:Polyethylene Glycols; D010544:Peroxidases; D002711:Chloride Peroxidase; D000645:Ammonium Sulfate