Production of immunoglobulins and alloantibodies by lymphocytes from lung cancer patients.
Spontaneous and PWM stimulated production of immunoglobulins (Igs) were measured in culture of lymphocytes isolated from peripheral blood (PBL) of lung cancer patients, blood donors and patients with benign lung diseases. Culture supernatants were tested for antibody activity against lung cancer cells and normal cells and some of them were absorbed with normal lung cells and fetal liver cells. Igs levels and antibody activity in culture supernatants were measured by radioimmunometric assay. Significantly increased level of Igs in unstimulated culture of PBL and low index of PWM-stimulated production of Igs was found in lung cancer patients in contrast to the controls. Supernatants of PBL cultures from lung cancer patients had significant low antibody activity to lung cancer cells and were effectively absorbed by normal lung cells and fetal liver cells. The results suggest in vivo polyclonal activation of B cells in patients with lung cancer and simultaneously diminished production of alloantibodies with some features of natural antibodies following polyclonal activation in vitro.
D000230:Adenocarcinoma / Q000276:immunology; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007518:Isoantibodies / Q000096:biosynthesis*; D008171:Lung Diseases / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values
D007136:Immunoglobulins; D007518:Isoantibodies