Production of leukemic blast growth factor by a human bladder carcinoma cell line.
Circulating blast cells from the peripheral blood of acute myeloblastic leukemia patients include a subpopulation capable of colony formation in the presence of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). We describe the complete replacement in the blast assay of PHA-LCM by conditioned medium from a human bladder carcinoma cell line, HTB9. Both conditioned media contain a stimulator of blast cell growth that elutes as a single peak from a Sephadex G100 column with an apparent molecular weight of 30,000. It is shown that this leukemic blast growth factor is distinct from erythroid-potentiating activity (EPA) and possibly granulocyte macrophage colony-stimulating factor.
D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D003470:Culture Media / Q000032:analysis; D006133:Growth Substances / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis; D010835:Phytohemagglutinins / Q000494:pharmacology; D015291:Transforming Growth Factors; D014410:Tumor Stem Cell Assay; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
D003470:Culture Media; D006133:Growth Substances; D010455:Peptides; D010835:Phytohemagglutinins; D015291:Transforming Growth Factors