The SV40 T-antigen gene can have two introns.
F8dl is an SV40 early-region mutant that lacks over 60% of the DNA sequences normally used to encode large T antigen. This mutant employs a novel splice donor junction at nucleotide 4425 to produce a family of doubly spliced messages. A similar splicing pattern with wild-type SV40 mRNA has been observed, indicating that the wild-type gene for T antigen can also have two introns. A single G-to-T base change at nucleotide 4425 is sufficient to eliminate this novel donor splice junction.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D051379:Mice; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D004247:DNA; D011494:Protein Kinases