Abrupt induction of a membrane digestive enzyme by its intraintestinal substrate.
The regulation of amino-oligopeptidase (AOP), an intestinal brush border hydrolase essential for the surface digestion of peptide nutrients, was examined in rats in vivo. Short-term (30-minute) intraintestinal perfusion of a tetrapeptide substrate, Gly-Leu-Gly-Gly, or a synthetic substrate, leucyl-beta-naphthylamide, induced a doubling in the incorporation of [3H]leucine into the AOP in association with intracellular membranes. The subsequent conversion of AOP from nascent to mature enzyme and its membrane-associated transport to the brush border occurred at normal rates.
D000626:Aminopeptidases / Q000096:biosynthesis*; D000818:Animals; D018826:CD13 Antigens; D004044:Dietary Proteins / Q000378:metabolism; D004063:Digestion; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004790:Enzyme Induction; D006056:Golgi Apparatus / Q000378:metabolism; D007422:Intestines / Q000201:enzymology*; D007700:Kinetics; D007930:Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000201:enzymology; D009842:Oligopeptides / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004044:Dietary Proteins; D009842:Oligopeptides; C040135:leucine-beta-naphthylamide; C043841:glycyl-leucyl-glycyl-glycine; D000626:Aminopeptidases; D018826:CD13 Antigens; D007930:Leucine