The use of colloidal gold for screening monoclonal antibodies to cell surface antigens.
An immunogold method in Terasaki plates is described which allows accurate and sensitive visualization of the binding of monoclonal antibodies to cell surface antigens and is suitable for large scale screening. Monolayers of fixed cells are prepared in the wells. The binding of monoclonal antibodies is detected by a protein A gold complex. The cell-bound gold can be visualized by either optical or transmission electron microscopy. The results obtained with various monoclonal antibodies are presented.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis*; D003102:Colloids; D006046:Gold; D051379:Mice; D008854:Microscopy, Electron; D013205:Staphylococcal Protein A
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003102:Colloids; D013205:Staphylococcal Protein A; D006046:Gold