Acylation of adenovirus type 12 early region 1b 18-kDa protein. Further evidence for its localisation in the cell membrane.
The 18-kDa E1b protein in Ad 12-transformed rat cells and in Ad 12-infected human cells binds lipid strongly. The lipid is not removed by boiling in the presence of SDS or by extraction with methanol/chloroform. It is, however, dissociated from the protein by treatment with methanolic KOH suggesting that attachment is through an ester linkage. The acylated 18-kDa protein is detected only in the membrane fraction. Labelling cell surface proteins on Ad 12-transformed cells with [125I]iodosulphanilic acid shows that some of the Ad 12 18-kDa E1b protein is present on the outside of the cell. It is concluded that this protein is responsible for cell surface T-antigen activity.
D000215:Acylation; D000256:Adenoviridae / Q000276:immunology; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000276:immunology / Q000378:metabolism*; D001921:Brain; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002472:Cell Transformation, Viral; D004622:Embryo, Mammalian; D006801:Humans; D006878:Hydroxides / Q000494:pharmacology; D007668:Kidney; D050356:Lipid Metabolism; D000432:Methanol / Q000494:pharmacology; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds; D051381:Rats; D014415:Tunicamycin / Q000494:pharmacology; D014764:Viral Proteins / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D006878:Hydroxides; D010169:Palmitic Acids; D017680:Potassium Compounds; D014764:Viral Proteins; D014415:Tunicamycin; D019308:Palmitic Acid; D011188:Potassium; C029943:potassium hydroxide; D000432:Methanol