Influence of sex hormones on prostaglandin dehydrogenase activity in the rat uterus.
The present experiments report the effects of estradiol or of progesterone on the activity of 15-prostaglandin-dehydrogenase (PGDH) in the uterus of spayed rats. When the substrate was PGF2 alpha the treatment with progesterone (4 mg X day-1, two days) or with estradiol-17-beta (0.5 ug + 1 ug) did not show any effect on the activity of the enzyme. On the contrary, uteri from ovariectomized rats injected with a higher dose of estradiol-17-beta (0.5 ug + 50 ug) exhibited a significant increment. When the substrate was PGE2, progesterone failed again to modify the enzyme activity, whereas estradiol, both at a low and at a high doses, enhanced significantly the uterine PGDH activity. The possibility of two different PGDHs for each PG and the role of estradiol in enhancing PGE2 catabolism into 15-keto-PGE2 as a mechanism subserving the effect of estrogens on the output of this PG in the rat uterus, are discussed.
D000818:Animals; D002369:Castration; D015237:Dinoprost; D015232:Dinoprostone; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D010053:Ovary / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology*; D011458:Prostaglandins E; D011460:Prostaglandins F; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D014599:Uterus / Q000187:drug effects* / Q000201:enzymology
D011458:Prostaglandins E; D011460:Prostaglandins F; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D015232:Dinoprostone