Detection of multiple serovars of Chlamydia trachomatis in genital infections.
Eight genital isolates of Chlamydia trachomatis that each contained two different serovars were identified by typing with serovar-specific monoclonal antibodies. Mixed infections were confirmed by using serovar-specific monoclonal antibodies in an indirect immunofluorescence assay. For some isolates, fluorescein/rhodamine double-label indirect immunofluorescence with mixtures of IgM and IgG monoclonal antibodies to Chlamydia provided direct visual confirmation of mixed infection. Two isolates contained serovars D and F, and two E and F; one isolate contained serovars Ba and E, one D and J, one I' and F, and one E and J. In a sample of 352 genital isolates of C. trachomatis consecutively typed by dot ELISA with monoclonal antibodies, seven (2%) demonstrated mixed serovars.
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006716:Homosexuality; D006801:Humans; D008297:Male; D012002:Rectal Diseases / Q000382:microbiology*; D012007:Rectum / Q000382:microbiology; D012703:Serotyping; D012749:Sexually Transmitted Diseases / Q000382:microbiology*; D002577:Uterine Cervical Diseases / Q000382:microbiology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal