Subcellular distribution of the immunoreactive peptide histidine isoleucine in the rat brain and release from synaptosomes in vitro.
Subcellular fractionation studies of rat brain homogenate revealed that peptide histidine isoleucine-like immunoreactivity (PHI-LI) was enriched in synaptosomal preparations. PHI-LI was released by high potassium concentrations from synaptosomal pellets incubated in vitro. These results suggest a possible physiological role of PHI in the central nervous system as a neurotransmitter or modulator of synaptic function.
D000818:Animals; D001923:Brain Chemistry; D004151:Dipeptides / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D010451:Peptide PHI; D011188:Potassium / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000032:analysis; D013574:Synaptosomes / Q000378:metabolism
D004151:Dipeptides; D010451:Peptide PHI; D011188:Potassium