The defect in delayed-type hypersensitivity of young adult SJL mice is due to a lack of functional antigen-presenting cells.
SJL mice exhibit a strain-specific age-dependent delay in the maturation of delayed-type hypersensitivity (DTH) responsiveness. They do not attain "adult" levels of DTH responsiveness until the 10th week of age, which is 4 to 6 weeks later than the other strains of mice tested. In this report we demonstrate that spleen cells, resident peritoneal cells and thioglycollate-elicited peritoneal exudate cells are all able to transfer DTH responsiveness from naive 12-week-old DTH responders to 6-week-old nonresponders. Transfer prior to immunization was more efficient at eliciting a response than transfer after immunization. As few as 5 X 10(4) cells from 12-week-old SJL mice can adoptively transfer responsiveness to unresponsive 6-week-old animals. The active cell was found to be adherent, radiation (2000 rds) resistant, I-A+, Thy-1- and Mac-1+. I-A compatibility between the adoptively transferred population and the nonresponder mice is required. These data suggest that young adult SJL mice lack a functional population of antigen-presenting cells specific for DTH and that the appearance of these cells is under maturational control.
D000375:Aging; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000502:physiology; D000941:Antigens / Q000008:administration & dosage; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL
D000941:Antigens; D000949:Histocompatibility Antigens Class II