Cell cycle effects of an autologous growth promoter from human leukemia cells.
The mechanism of action of an autologous growth promoter, produced by HL-60 cells grown in serum-free defined medium, was investigated. Determinations of cell cycle phase distribution, which showed an approx. 16% increase in S and G2M with a reciprocal decrease in G1 cells, indicated a mechanism causing decreased time in G1, resulting in a more rapid entry of cells into G2M. Adsorption of the culture supernatant with HL-60 cells resulted in a decreased promoter activity, suggesting an autologous receptor mechanism for the promoter.
D001854:Bone Marrow Cells; D002453:Cell Cycle; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003470:Culture Media; D005434:Flow Cytometry; D006133:Growth Substances / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007399:Interphase; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism
D003470:Culture Media; D006133:Growth Substances; D013936:Thymidine