Mouse monoclonal antibodies with specificity for the melanoma-associated ganglioside disialyllactosylceramide (GD3) also react with the structural analogue disialylparagloboside.
A mouse monoclonal IgM antibody, 4.2, has previously been shown to bind preferentially to the surface of human malignant melanoma cells and to have specificity for the GD3 ganglioside (NeuAc alpha 2----8NeuAc alpha 2----3Gal beta 1----4GlcCer). Using overlay of antibodies on thin-layer chromatograms with glycolipids of various sources, it was shown that antibody 4.2, a further IgM and two IgG3 mouse monoclonal antibodies, selected on the basis of reactivity with GD3, also bound with similar strength to the structural analogue NeuAc alpha 2----8NeuAc alpha 2----3Gal beta 1----4GlcNac beta 1----3Gal beta 1----4GlcCer or disialylparagloboside. The SK-MEL 28 melanoma cell line used for immunization was shown to contain a large amount of GD3 but to lack disialylparagloboside. The demonstrated cross-reactivity may be of importance when considering the use of these antibodies for biochemical and medical purposes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D002460:Cell Line; D002855:Chromatography, Thin Layer; D003429:Cross Reactions; D005732:Gangliosides / Q000276:immunology*; D005915:Globosides / Q000276:immunology*; D006028:Glycosphingolipids / Q000276:immunology*; D006801:Humans; D008545:Melanoma / Q000032:analysis*; D051379:Mice; D051381:Rats
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D005915:Globosides; D006028:Glycosphingolipids; C026226:ganglioside, GD3; C018928:disialosyl paragloboside