Defective chemotaxis and adherence in granulocytes from chronic myeloid leukemia (CML) patients.
Adherence and motility of granulocytes from chronic myeloid leukemia (CML) patients was compared with that of granulocytes from normal subjects. The percentage of non-adherent granulocytes was significantly higher in untreated CML patients and patients in relapse and acute blastic crisis (ABC) (P less than 0.01). Chemotactic Index (C.I.) of granulocytes moving in a gradient of a synthetic chemoattractant F-Met-Leu-Phe was measured by time-lapse cinématography. While 84.5 +/- 3.53% of normal granulocytes were motile, only 30.3 +/- 14.7% granulocytes from untreated patients, 33.8 +/- 21.3% granulocytes from relapse patients and 36 +/- 9.9% granulocytes from ABC patients were found to be motile. The C.I. of motile granulocytes from CML patients was significantly lower in untreated patients (P less than 0.05), in patients in relapse (P less than 0.01) and in patients in ABC (P less than 0.05), as compared to that of normal granulocytes. Visualization of cytoplasmic actin by indirect immunofluorescence, revealed the presence of actin in granulocytes from patients in all stages of the disease. Thus, granulocytes from CML patients were defective in directional locomotion. Organized actin filaments were found in the small percentage of motile cells still found in CML patients.
D000199:Actins / Q000097:blood; D002448:Cell Adhesion; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid / Q000097:blood*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D012008:Recurrence
D000199:Actins; D009240:N-Formylmethionine Leucyl-Phenylalanine