Studies on platelet surface carbohydrates in normal and uraemic platelets using 125I-labelled lectins.
Binding studies with six different purified 125I-labelled lectins, concanavalin A (con A), wheat germ agglutinin (WGA), Ricinus communis agglutinin II (RCA II), Dolichos biflorus (DB), Tetranolobus purpureus (TP) and P-phyto-hemagglutinin (P-PHA), were used to investigate the surface topography of carbohydrates in platelets from uraemic and normal subjects. Compared with normal the uraemic platelets, bear significantly decreased (more than 2.5-fold) numbers of receptors for P-PHA (N-acetyl D-galactosamine specificity) and Con A (specificity glucose, mannose). The number of WGA, RCA, II, DB and TP receptors in uraemic platelets did not differ from the number in normal platelets. Binding studies with 125I-labelled lectins provide further evidence of molecular defects in uraemic platelets. Moreover, this method might provide a fast and reliable technique for identifying abnormalities in the surface topography of carbohydrates on platelets in several pathological states.
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008297:Male; D010835:Phytohemagglutinins / Q000378:metabolism; D011975:Receptors, Mitogen / Q000032:analysis*; D014511:Uremia / Q000097:blood*
D002241:Carbohydrates; D037102:Lectins; D010835:Phytohemagglutinins; D011975:Receptors, Mitogen; D003208:Concanavalin A