The effects of lead on differentiation of the Friend leukemia cells and rat bone marrow cells.
The effects of lead toxicity on differentiation of erythroid cells was examined using Friend leukemia cells induced with 2% dimethyl sulfoxide. By increasing the concentration of lead, these cells exhibited a lag before the onset of induction. When incorporation of [3H]-delta-aminolevulinic acid into heme was compared, the maximum incorporation into the cells without lead was on the third day after induction, while it was on the fifth day in the presence of lead (5 X 10(-4) M). However, these cells did reach similar differentiation stages by the seventh day. The amount of delta-aminolevulinic acid which was excreted into the medium increased in the presence of more than 10(-4) M lead. The amount of coproporphyrin III was slightly increased with 10(-6) M lead. Protoporphyrin IX content in the cells decreased slightly with increasing concentrations of lead. delta-Aminolevulinic acid dehydratase activity in cells which were cultured in the presence of lead was low, while synthesis of this enzyme increased in the presence of lead. The bone marrow cells from lead-poisoned rats exhibited a lag in onset of maturation, which was consistent with the effect of lead on Friend cells.
D000622:Aminolevulinic Acid / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003306:Coproporphyrins / Q000378:metabolism; D004920:Erythropoiesis / Q000187:drug effects*; D005622:Friend murine leukemia virus; D007854:Lead / Q000633:toxicity*; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D008297:Male; D000623:Porphobilinogen Synthase / Q000032:analysis; D011524:Protoporphyrins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D003306:Coproporphyrins; D011524:Protoporphyrins; D007854:Lead; D000622:Aminolevulinic Acid; D000623:Porphobilinogen Synthase