Cell kinetics of the K-562 cell line in microculture.
The cell kinetic parameters of K-562 leukemia cells were studied using microwell cultures in which growth was initiated from a single cell. Total population growth was studied by direct enumeration, 3H-thymidine labelling, and flow cytometry. Clonogenic cell growth was studied by replating and 3H-thymidine suicide. In 7-day clones of K-562 cells, durations of the total cell cycle, G1, S, G2, and M phases were 20.8 h, 3.5 h, 12.9 h, 3.3 h, and 1.1 h, respectively; the growth fraction was 0.92 and the cell loss factor was 0.084. Study of colony-forming cells by replating indicated that clonogenic cells comprised 40% of total cells. 3H-Thymidine suicide showed that cell-cycle duration for these cells was 22.5 h and that S-phase duration was 11.7 h.
D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells / Q000473:pathology; D046508:Culture Techniques / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid / Q000473:pathology*; D013997:Time Factors; D014410:Tumor Stem Cell Assay
D004273:DNA, Neoplasm