Probing the combining site of monoclonal IgA J539 using deoxyfluoro- and other galactosides as ligands.
IgA J539 is a monoclonal anti beta-(1----6)-D-galactopyranan. Measurement of its affinity with a number of synthetic galactosyl oligosaccharides of that linkage type, in which one or more hydroxyl groups have been replaced by a fluorine atom, confirm the assignment of the relative binding strength of the subsites in the protein for the individual galactosyl residues of the antigen.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D001666:Binding Sites, Antibody; D002236:Carbohydrate Conformation; D005685:Galactans / Q000276:immunology; D005697:Galactosides / Q000276:immunology*; D006027:Glycosides / Q000276:immunology*; D007070:Immunoglobulin A / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D008024:Ligands
D000911:Antibodies, Monoclonal; D005685:Galactans; D005697:Galactosides; D006027:Glycosides; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D008024:Ligands; C044347:1,6-galactan