[Biochemically labelled human lymphoblastoid cell line. I. Its karyotypic, growth and physiological characteristics].
A study was made of the properties of human lymphoid cell line RPMI-6410 derived from peripheral blood of a patient with acute myeloblastic leukaemia. The lymphoblastoid cell line was found to be resistant to 5-brom-deoxyuridine and to have a low thymidine kinase activity. The modal chromosome number for RPMI-6410 is 46-47 XY. The karyotype includes marker chromosomes: two large submetacentrics --M1 and M2, and two small acrocentrics--M3 and M4. Ways of marker chromosome formation are discussed. The properties of RPMI-6410 line make it possible to use it for somatic cell hybridization, in particular, for obtaining hybridoma synthesizing human monoclonal antibodies.
D002455:Cell Division; D002459:Cell Fusion; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000097:blood; D008214:Lymphocytes / Q000502:physiology / Q000648:ultrastructure*; D009154:Mutation; D013937:Thymidine Kinase / Q000378:metabolism
D003470:Culture Media; D013937:Thymidine Kinase
Biokhimicheski markirovannaia limfoblastoidnaia liniia kletok cheloveka. I. Kariotipicheskaia, rostovye i fiziologicheskie kharakteristiki.