An assay for screening drugs against animal-infective bloodstream forms of Trypanosoma brucei brucei in vitro.
A rapid and simple assay for in vitro drug screening has been established using the mammalian feeder layer cell system for continuous cultivation of infective bloodstream forms of Trypanosoma brucei brucei. A total of 21 trypanocides have been tested. Differences in sensitivity to standard trypanocides were detected among drug-susceptible isolates and strains of T. b. brucei. This assay may thus be of potential use for rapid detection of new drug-resistant isolates of T. b. brucei.
D000818:Animals; D004133:Diminazene / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D010274:Parasitology / Q000379:methods; D011806:Quinolinium Compounds / Q000494:pharmacology; D013498:Suramin / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects*
D011806:Quinolinium Compounds; D014344:Trypanocidal Agents; D013498:Suramin; C005707:quinapyramine; C003915:diminazene aceturate; D004133:Diminazene