Density distribution of chronic myeloid leukaemia and normal colony-forming cells in diffusion chambers (CFU-D) and agar (CFU-GM).
The density distribution in Percoll gradients of clonogenic cells forming colonies in diffusion chambers (CFU-D) or in agar culture (CFU-GM) was studied in chronic myeloid leukaemia (CML). The density distribution of CFU-GM in CML was homogeneous with peaks within 1.058-1.061 g/ml, which is slightly lower than normal. CFU-D, on the other hand, showed heterogeneous distributions both in CML and in normal controls. Two separable populations of CFU-D were recognized, one with the same or lower density than CFU-GM that formed almost exclusively neutrophilic colonies in diffusion chambers, and a second population concentrating in the density range 1.068-1.075 g/ml which primarily formed macrophage colonies. The second population contained colony-forming cells derived from both Fc-receptor positive and Fc-receptor negative precursor cells, suggesting that at least some colonies in diffusion chambers arise from Fc-receptor positive granulopoietic cells of intermediate maturity and/or monocytes. The concentration of CFU-D in peripheral blood was increased 40- to 100-fold in 2 patients currently off treatment who had increased WBC counts.
D000362:Agar; D001853:Bone Marrow / Q000473:pathology; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques / Q000379:methods; D004058:Diffusion; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000276:immunology; D011961:Receptors, Fc / Q000032:analysis; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology
D011961:Receptors, Fc; D000362:Agar