Relationship of c-myc expression to differentiation and proliferation of HL-60 cells.
We have compared changes in c-myc expression in HL-60 promyelocytic leukemia cells induced to differentiate by dimethyl sulfoxide or growth inhibited in an undifferentiated state. Under these conditions, c-myc expression did not correlate with the proportion of proliferating cells. The kinetics of the decrease in c-myc expression upon differentiation induction is paralleled closely by an increasing proportion of histochemically detected differentiated myeloid cells and by a decrease in clonogenic potential but not by changes in the proportion of proliferating cells. Changes in c-myc expression subsequent to differentiation induction can therefore be directly related to the differentiation process rather than to a cell cycle-related phenomenon.
D002454:Cell Differentiation; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D004121:Dimethyl Sulfoxide