Elastase digested urokinase (ED-UK).
Elastase digested urokinase (ED-UK) was prepared from human high mol. wt urokinase (HMW-UK). It resembled low mol. wt urokinase (LMW-UK) in its mol. wt, specific activity, and active sites. The steady-state kinetic parameters of each enzyme for the activation of human Glu-plasminogen also resembled each other, as did their amidase parameters (with pyro-Glu-Gly-Arg-pNA).
D002846:Chromatography, Affinity; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D009842:Oligopeptides / Q000378:metabolism; D010196:Pancreatic Elastase; D010958:Plasminogen / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis* / Q000378:metabolism
D009842:Oligopeptides; C018788:valyl-leucyl-lysine 4-nitroanilide; C028190:5-oxo-prolyl-glycyl-arginine-4-nitroanilide; D010958:Plasminogen; D010196:Pancreatic Elastase; D014568:Urokinase-Type Plasminogen Activator