Role of transferrin in metal uptake by human lymphoblasts in vitro.
The uptake and binding of 59Fe, 67Ga and 239Pu complexed with citrate of transferrin (Tf) and of 125I-labelled Fe-Tf by human lymphoblasts (WI-L2 cells) have been studied. Uptake kinetics of 59Fe-Tf and [125I]-Tf point to internalization by receptor mediated endocytosis. 67Ga binding and uptake is always less. This may be explained by a lower affinity of Ga-complexes for the cell surface. Factors which influence Fe uptake have a similar effect on Ga. 239Pu uptake and binding, however, are different, especially in that Tf does not stimulate 239Pu uptake and may actually decrease it.
D002460:Cell Line; D002951:Citrates / Q000378:metabolism / Q000494:pharmacology; D019343:Citric Acid; D005708:Gallium / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D008214:Lymphocytes / Q000254:growth & development / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D011005:Plutonium / Q000378:metabolism; D011485:Protein Binding; D011868:Radioisotopes; D013154:Spleen / Q000166:cytology; D014168:Transferrin / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*
D002951:Citrates; D008670:Metals; D011868:Radioisotopes; D014168:Transferrin; D019343:Citric Acid; D011005:Plutonium; D005708:Gallium; D007501:Iron