Effect of alpha-chlorohydrin on metabolism and testosterone secretion by rat testicular interstitial cells.
The direct effect of alpha-chlorochydrin (alpha-CH) on basic metabolism (glucose utilization and oxygen consumption) and testosterone secretion by isolated rat interstitial cells (I-cells) has been studied. In the range of concentrations between 5 and 100 microliter/ml, only the highest doses of alpha-CH decreased cell vitality and their histochemical stain for 3 beta-HSD. Oxygen consumption of I-cells was depressed at all doses higher than 10 microliter/ml and this effect was reversible only with doses lower than 50 microliter/ml. glucose utilization by I-cells was depressed significantly by alpha-CH and this effect was particularly dramatic with doses higher than 50 microliter/ml. alpha-CH decreased testosterone secretion by I-cells, with maximal effects at 100 microliter/ml. I-cells responded to hCG challenge by increasing testosterone secretion, and hCG prevented the toxic effect of alpha-CH at the lowest dose (10 microliter/ml) of alpha-CH, but failed to overcome the effects of a high dose (100 microliter/ml).
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D002728:Chlorohydrins / Q000494:pharmacology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism*; D000517:alpha-Chlorohydrin / Q000494:pharmacology*
D002728:Chlorohydrins; D006063:Chorionic Gonadotropin; D013739:Testosterone; D000517:alpha-Chlorohydrin; D015096:3-Hydroxysteroid Dehydrogenases; D005947:Glucose