ATP and 2,3-diphosphoglycerate changes in the erythrocytes of hyperammonaemic sheep.
The experiment was conducted on sheep receiving NH4Cl infusions into v. mesentericta anterior. During first 3 h animals received ammonia in a dose of 13 mumol X min-1 X kg body wt.-1 and then next 3 h in a dose of 30 mumol X min-1 X kg body wt.-1. When ammonia was administered in smaller doses its concentration increased to 0.65 and 0.22 mmol/l in portal and peripheral blood, respectively. Simultaneous slight increase of ATP and marked of 2,3-diphosphoglycerate (2,3-DPG) was observed. These effects of moderate ammonia loading are probably caused by the rise of glucose utilization in erythrocytes. Ammonia given in a higher dose induced further rise of its concentration to 1.1 and 0.51 mmol/l in portal and peripheral blood, respectively. Such NH4Cl infusion caused a further rise of 2,3-DPG concentration connected with the drop of ATP level in ovine red blood cells. It seems that a decrease of ATP level was mainly the result of local high 2,3-DPG level which inhibits glycolytic enzymes.
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood*; D000641:Ammonia / Q000097:blood* / Q000494:pharmacology; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D009994:Osmolar Concentration; D011169:Portal Vein; D012756:Sheep
D004163:Diphosphoglyceric Acids; D000643:Ammonium Chloride; D019794:2,3-Diphosphoglycerate; D000641:Ammonia; D000255:Adenosine Triphosphate