Characterization of saliva proteins from 'stainers' and 'non-stainers' adsorbed to hydroxyapatite.
Stainers and non-stainers were selected on the basis of their individual tendency to develop extrinsic tooth discolorations from a chlorhexidine mouth rinse. Saliva proteins adsorbed to hydroxyapatite in vitro and in vivo pellicle from the participants were analyzed by gel filtration and ion-exchange chromatography. Two distinct anionic components were isolated. The elution patterns from stainers and non-stainers were identical. Amino acid analyses of the main peaks demonstrated a prevalence of serine, glycine, and glutamic acid.
D000042:Absorption; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003741:Dental Deposits / Q000032:analysis; D044622:Dental Pellicle; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D006882:Hydroxyapatites; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000378:metabolism*; D014075:Tooth Discoloration / Q000378:metabolism*
D000596:Amino Acids; D006023:Glycoproteins; D006882:Hydroxyapatites; D012471:Salivary Proteins and Peptides