Soluble chromogenic substrates for the assay of endo-1,4-beta-xylanases and endo-1,4-beta-glucanases.
New soluble chromogenic substrates were prepared for specific and rapid assays of endo-1,4-beta-xylanases and endo-1,4-beta-glucanases. A soluble beechwood 4-O-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R, and hydroxyethylcellulose was coupled to Ostazin brilliant red H-3B. The assays are based on photometric measurements of the enzyme-released dyed fragments soluble in the presence of organic solvents which precipitate the original substrates and their high-molecular-weight fractions. The assays are advantageous for rapid analyses of large amount of samples and also permit evaluation of the activities of both enzymes in the presence of exo-beta-glycanases and beta-glycosidases, at a high level of reducing compounds and viable cells, on the cell surface and on cell membranes and organelles.
D001234:Aspergillus niger / Q000201:enzymology; D002480:Cellulase / Q000032:analysis*; D002482:Cellulose / Q000031:analogs & derivatives; D002863:Chromogenic Compounds / Q000138:chemical synthesis*; D004396:Coloring Agents; D043364:Endo-1,4-beta Xylanases; D006026:Glycoside Hydrolases / Q000032:analysis*; D012995:Solubility; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology; D014990:Xylans
D002863:Chromogenic Compounds; D004396:Coloring Agents; D014990:Xylans; D002482:Cellulose; C002283:hydroxyethylcellulose; C044967:4-O-methyl glucuronoxylan; D006026:Glycoside Hydrolases; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases