Antibiotic accumulation in human polymorphonuclear leucocytes and lymphocytes.
Human polymorphonuclear leucocytes and lymphocytes were incubated with 10 different radiolabelled antibiotics (10 micrograms/ml) for 2 hours. The ratio of cellular concentration to extracellular concentration of the drugs was determined after centrifugation. For beta-lactam antibiotics and gentamicin the ratios were less than 1.0. The ratios for doxycycline, erythromycin, fusidic acid and rifampicin were 13, 4.3, 7-10 and 2.5 respectively indicating a cellular accumulation of these drugs. Erythromycin, fusidic acid and rifampicin were rapidly released from the cells in antibiotic free medium, while doxycycline was partly irreversibly bound.
D000900:Anti-Bacterial Agents / Q000097:blood* / Q000627:therapeutic use; D006105:Granulomatous Disease, Chronic / Q000188:drug therapy; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*
D000900:Anti-Bacterial Agents