Presence of nucleosomal repeat in the transcribed alpha globin gene of induced murine erythroleukemia cells.
The sensitivity of the mouse alpha-globin gene to micrococcal nuclease and its nucleosomal repeat were studied in three different functional states of the gene: inactive in EAT cells, potentially active in uninduced MEL cells and active in induced MEL cells. The results show that: 1. The nuclease sensitivity of the gene differs in the three different functional states. 2. Both the coding and the 5'-flanking regions of the induced actively transcribed gene show a typical nucleosomal repeat pattern. 3. Hypersensitive sites for micrococcal nuclease and for an endogenous nuclease appear upstream of the gene after induction of differentiation.
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D008040:Genetic Linkage; D005914:Globins / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D051379:Mice; D008836:Micrococcal Nuclease; D009707:Nucleosomes / Q000648:ultrastructure*; D014158:Transcription, Genetic / Q000187:drug effects
D002843:Chromatin; D009707:Nucleosomes; D005914:Globins; D008836:Micrococcal Nuclease; D004121:Dimethyl Sulfoxide