Immunoglobulin binding to platelets. The effect of aggregated IgG.
When fresh platelets were incubated in serum to which heat-aggregated IgG was added, the increase of platelet-associated IgG was paralleled by increased levels of platelet-associated IgM, IgA and albumin as determined by ELISA. The increase was observed regardless of whether the platelets were quantified by conventional platelet counting or with the help of 51Cr-labelling, which was done to avoid potential counting errors induced by platelet fragmentation. In thrombocytopenic patients a correlation between in vivo bound platelet-associated albumin and IgG could be confirmed. It was concluded that aggregated IgG induces an increased binding of serum immunoglobulins and albumin to platelets in vitro.
D001792:Blood Platelets / Q000276:immunology*; D002860:Chromium Radioisotopes; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology; D013921:Thrombocytopenia / Q000097:blood / Q000276:immunology
D002860:Chromium Radioisotopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012709:Serum Albumin