The S-sulfate formation from 4-nitrobenzyl mercaptan in rat liver cytosol.
4-Nitrobenzyl mercaptan (NBM) was enzymatically transformed at pH 6.0 into its S-sulfate in rat liver cytosol fortified with 3'-phosphoadenosine 5'-phosphosulfate. At pH 7.4, the S-sulfate was not detected from the incubation mixture. 4-Nitrobenzyl alcohol was also transformed under the same incubation conditions into the corresponding O-sulfate at a higher rate at pH 6.0 than at pH 7.4. Under the incubation conditions used, NBM S-sulfate reacted with the substrate NBM at a significant rate to afford 4-nitrobenzyl disulfide. The disulfide formation from NBM and the S-sulfate occurred more readily at pH 7.4 than at pH 6.0, so that biologically formed NBM S-sulfate was strongly suggested not to remain unchanged in the incubation mixture at pH 7.4.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000201:enzymology; D004220:Disulfides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D010724:Phosphoadenosine Phosphosulfate / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013466:Sulfurtransferases / Q000378:metabolism
D004220:Disulfides; D013431:Sulfates; D013438:Sulfhydryl Compounds; C046806:4-nitrobenzyl mercaptan; D010724:Phosphoadenosine Phosphosulfate; D013466:Sulfurtransferases