The differentiation of HL-60 cells in the synthetic medium induced by GM3 ganglioside.
GM3 ganglioside, added exogenously to a promyelocytic leukemia cell line (HL-60 cells) in serum-free synthetic medium, induced differentiation into macrophage-like cells. Macrophagic morphology and function of differentiation-induced cells were determined by cell growth behavior, May-Grünwald-Giemsa staining, activities of nonspecific esterase, phagocytosis and nitroblue tetrazolium (NBT) reduction. GM3 ganglioside may play a role in triggering differentiation of HL-60 cells into macrophage-like cells.
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D004950:Esterases / Q000378:metabolism; D005679:G(M3) Ganglioside / Q000494:pharmacology*; D005732:Gangliosides / Q000494:pharmacology*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology* / Q000503:physiopathology; D008264:Macrophages / Q000473:pathology / Q000502:physiology; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects
D003470:Culture Media; D005679:G(M3) Ganglioside; D005732:Gangliosides; D009580:Nitroblue Tetrazolium; D004950:Esterases