[Separation of the transforming growth factor from cells of murine sarcoma induced by polymeric film].
Use was made of the cell line PS-103 induced by subcutaneous implantation of plastic film to CBA mice. The conditioned medium from PS-103 was shown to stimulate anchorage independence of PS-103 itself and several pseudonormal cells: NRK and NIH/3T3. The peak of the colony-stimulating activity after gel filtration was displayed by fractions with a molecular weight of 15 000, which were acid- and heat-resistant. All those characteristics correlated with the traits of the transforming growth factors (TGF). The significance of the TGF for carcinogenesis induced by plastic films is discussed.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D066298:In Vitro Techniques; D051379:Mice; D008808:Mice, Inbred CBA; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D011143:Polyvinyl Chloride; D012513:Sarcoma, Experimental / Q000139:chemically induced; D015291:Transforming Growth Factors
D009363:Neoplasm Proteins; D010455:Peptides; D015291:Transforming Growth Factors; D011143:Polyvinyl Chloride
Vydelenie kletkami myshinoĭ sarkomy indutsirovannoĭ polimernoĭ plastinkoĭ, transformiruiushchego faktora rosta.