Immunogenicity of Corynebacterium pseudotuberculosis and the effect of adjuvants in mice.
Intraperitoneal inoculation of CF1 mice with 100 micrograms, 200 micrograms, or 300 micrograms whole cell (WC) or 250 micrograms, 500 micrograms, or 1000 micrograms cell wall (CW) of Corynebacterium pseudotuberculosis induced varying degrees of protection after intravenous challenge of immunity with 7.2 X 10(4) CFU of C. pseudotuberculosis. Generally, the degree of protection increased with the dose of WC or CW. However, intraperitoneal inoculation of mice with 100 micrograms, 200 micrograms, or 300 micrograms heat-killed Mycobacterium bovis BCG; 50 micrograms, 150 micrograms, or 300 micrograms of either muramyl dipeptide (MDP) or trehalose dimycolate (TDM); or 350 micrograms, 700 micrograms or 1400 micrograms Corynebacterium parvum did not induce resistance to intravenous inoculation of 7.4 X 10(4) CFU of C. pseudotuberculosis. The protection induced by 500 micrograms CW was enhanced by adding 100 micrograms BCG, 150 micrograms MDP, or 350 micrograms C. parvum but protection induced in mice by 300 micrograms of WC was not enhanced by adding any adjuvants.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000276:immunology; D000276:Adjuvants, Immunologic; D000818:Animals; D001500:BCG Vaccine; D002473:Cell Wall / Q000276:immunology; D003311:Cord Factors / Q000276:immunology; D003352:Corynebacterium / Q000276:immunology*; D003354:Corynebacterium Infections / Q000276:immunology / Q000662:veterinary*; D005260:Female; D005620:Freund's Adjuvant; D007114:Immunization / Q000662:veterinary*; D051379:Mice; D011425:Propionibacterium acnes / Q000276:immunology
D000276:Adjuvants, Immunologic; D001500:BCG Vaccine; D003311:Cord Factors; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D005620:Freund's Adjuvant