Elevated chondroitin 6-sulfotransferase activity in fetal bovine serum.
Assay conditions for chondroitin sulfotransferase in serum were established using a rapid and simple paper disk method. Sulfotransferase activities towards endogenous serum glycosaminoglycans and exogenously added chondroitin were determined for fetal, newborn and adult bovine sera. The results indicate that 3'-phosphoadenosine 5'-phosphosulfate (PAPS); chondroitin 6-sulfotransferase activity in fetal calf serum is several times that in newborn or adult bovine sera. The enzyme transfers sulfate onto position 6 of internal nonsulfated galactosamine units of endogenous and exogenous chondroitin.
D000375:Aging; D000818:Animals; D002417:Cattle; D002807:Chondroitin / Q000378:metabolism; D005312:Fetal Blood / Q000201:enzymology*; D006025:Glycosaminoglycans / Q000097:blood; D006801:Humans; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000097:blood*
D006025:Glycosaminoglycans; D013431:Sulfates; D010724:Phosphoadenosine Phosphosulfate; D002807:Chondroitin; D013466:Sulfurtransferases; D015238:Sulfotransferases; C019893:chondroitin 4-sulfotransferase