Slow- and tight-binding inhibition of aspartate aminotransferase by L-hydrazinosuccinate.
The inhibition of aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1) by L-hydrazinosuccinate has been studied. The velocity of the enzyme reaction decreased with time when the reaction was initiated by the addition of enzyme to a mixture of the assay components and L-hydrazinosuccinate, while it increased slowly from a low level when a preincubated mixture of the enzyme and the inhibitor was added to the reaction mixture to initiate the reaction. Nearly 50% decrease in the initial reaction velocity was produced by a prolonged preincubation of the enzyme with the inhibitor, both at low concentrations of about 2 nM. These findings indicate that the inhibition is of the slow- and tight-binding type. The time-course of the reaction of the enzyme and the inhibitor, examined by the change in activity, was not in accord with single-step mechanisms, but rather appeared to follow biphasic kinetics. The inhibition could be fully reversed only in the presence of L-cysteine sulfinate or large excess of L-aspartate to convert the regenerated enzyme to its pyridoxamine form. The time-course of the reversal followed pseudo-first-order kinetics. Quantitative analysis of the experimental data has shown that the results are consistent with a mechanism of enzyme-inhibitor interaction which involves a reaction of two consecutive, reversible steps. The overall inhibition constant for L-hydrazinosuccinate was calculated to be approx. 0.2 nM.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors*; D006834:Hydrazines / Q000494:pharmacology*; D007700:Kinetics; D008433:Mathematics; D009206:Myocardium / Q000201:enzymology; D013386:Succinates; D013552:Swine; D013997:Time Factors
D006834:Hydrazines; D013386:Succinates; C042747:hydrazinosuccinic acid; D001219:Aspartate Aminotransferases