Strand break repair, DNA polymerase activity and heat radiosensitization in thermotolerant cells.
HeLa S3 cells were made thermotolerant by 'chronic' (5 h at 42 degrees C) or 'acute' (15 min at 44 degrees C followed by 5 h at 37 degrees C) heat treatments. Cell survival, repair of radiation-induced DNA strand breaks, alpha and beta DNA polymerase activity and radiation sensitivity following hyperthermia were all measured in both control and thermotolerant cells. The ability to repair DNA strand breaks correlated well with cell survival following hyperthermia. Hyperthermic inhibition of strand break repair was reduced in thermotolerant relative to control cells, although the thermal tolerance ratios for repair were less than for hyperthermic cell killing. Both radiosensitization and DNA polymerase inactivation by hyperthermia were only slightly reduced in thermotolerant relative to control cells. Hence a poor correlation was found between these two parameters and hyperthermic cell survival. For all heat treatments applied, alpha and beta DNA polymerase activity correlated well with the extent of hyperthermic radiosensitization.
D002470:Cell Survival / Q000528:radiation effects; D004256:DNA Polymerase I / Q000378:metabolism / Q000528:radiation effects; D004257:DNA Polymerase II / Q000378:metabolism / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism* / Q000528:radiation effects; D006367:HeLa Cells / Q000201:enzymology / Q000528:radiation effects; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D011836:Radiation Tolerance; D013696:Temperature; D013997:Time Factors
D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase