The rates of replicative and unscheduled DNA synthesis as functions of temperature in LS/B1, L-As and L-C3 cells.
The relative rates of replicative and unscheduled DNA synthesis were determined over a temperature range of 2 degrees to 42 degrees C in LS/B1, L-As and L-C3 cells. The rates were determined by measuring the amount of 3H-dTh incorporated into DNA during 60 min incubation of cells at a given temperature. Acid-resistant 3H-activity incorporated into DNA at 2 degrees C was evaluated as evidence of DNA turnover, not non-specific adsorption of 3H-dTh. The relative rates of RDS and UDS were expressed as functions of temperature and from the data the values of Q10 and Eact necessary for incorporation of one mole of dTh into DNA during RDS and UDS were calculated. The values of Q10 and Eact are comparable to those reported for HeLa, V-79, L5/78Y and the epithelial cells. The experimentally determined values of Q10 and Eact for UDS in the lines tested did not vary. UDS, compared to RDS, is evaluated as a process with lesser demands for energy.
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D007700:Kinetics; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D013696:Temperature; D013816:Thermodynamics; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine