Epicardial image analysis using a desk top computer. Comparison of epicardial flow distribution and NADH-fluorescence pattern.
In isolated coronary-ligated rabbit hearts, quantification of epicardial flow distribution pattern and of ischemic area was performed from flow indicator and endogenous NADH-fluorescence pictures, respectively, after UV-flash photography. After being digitized (256 X 256 pixels), the fluorescence photos were analyzed by an Apple desk computer using self-written software (fast machine routines called from BASIC programs). The ischemic area (= sum of pixels with enhanced NADH fluorescence) corresponds to the area with disturbed coronary perfusion (= sum of pixels with diminished indicator fluorescence). Since coronary-active drugs might affect both parameters--local myocardial perfusion and metabolism--differentially, the method presented seems to be a very useful technique for differentiated analysis of the action profile of coronary-active drugs.
D000818:Animals; D003201:Computers; D003326:Coronary Circulation; D003327:Coronary Disease / Q000503:physiopathology; D005453:Fluorescence; D008838:Microcomputers; D009206:Myocardium / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D010101:Oxygen Consumption; D011817:Rabbits; D012984:Software
D009243:NAD