Cytotoxicity-related alterations of selected cellular functions after in vitro vanadate exposure.
A bovine kidney cell line was used to monitor select cellular functions for toxicity-dependent alterations in an effort to examine the cellular response to vanadium insult. The vanadium concentrations utilized ranged between 20 and 500 microM Na3VO4 (V) and elicited 15-60% cytotoxicity. Cytotoxicity-related decreases in thymidine incorporation into DNA and leucine incorporation into protein were noted. Paradoxically, V-treated cultures exhibited increased protein and DNA content, suggestive of a decrease in precursor transport. K+-dependent phosphatase (KP), acid phosphatase (AP) and succinate dehydrogenase (SDH) were monitored in surviving cells and in a cell-free system. Significant inhibitions were detected for KP and AP; SDH exhibited slight enhancement. In the cell-free system, KP was inhibited significantly at 10(-7) M V, while AP and SDH were either unchanged or sensitive only at concentrations of 10(-4) M V or greater. Measurement of reduced glutathione (GSH) in surviving cells revealed toxicity-dependent increases of up to 500% of control values. When compared to the cellular V content, the GSH:V molar ratio decreased from 1.7 to 0.5 as cell survival decreased.
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D007668:Kidney / Q000187:drug effects; D011506:Proteins / Q000032:analysis; D014638:Vanadates; D014639:Vanadium / Q000633:toxicity*
D011506:Proteins; D014639:Vanadium; D014638:Vanadates; D004247:DNA; D000469:Alkaline Phosphatase; D005978:Glutathione