Estrogen effects on sister chromatid exchanges in mouse uterine cervical and kidney cells.
Uterine cervical fibroblasts and kidney cells from 3-to 4-day-old, previously untreated or phenobarbital [(PBA) CAS: 50-06-6]-treated female NMRI mice were cultured in vitro in the presence of diethylstilbestrol [(DES) CAS: 56-53-1] or 17 beta-estradiol [(E2) CAS: 50-28-2]. DES at 10(-7) M in the culture medium increased the number of sister chromatid exchanges (SCE); no further increases was obtained with higher concentrations of DES. Pretreatment of the females with PBA resulted in an increase in SCE when DES was used in the range of 10(-7) to 10(-5) M. The level of this increase was significantly higher than that in fibroblasts from untreated females. E2 had no effect. DES had no effect on SCE in kidney cells from PBA-treated females. Indomethacin and alpha-naphthoflavone reduced the number of SCE in PBA-exposed fibroblasts to an intermediate level. The presence of both enzyme inhibitors in the medium depressed the number of SCE to the control level.
D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000187:drug effects* / Q000648:ultrastructure; D004054:Diethylstilbestrol / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000633:toxicity; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D010634:Phenobarbital / Q000494:pharmacology; D011247:Pregnancy; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D001571:Benzoflavones; D004958:Estradiol; C011512:alpha-naphthoflavone; D004054:Diethylstilbestrol; D007213:Indomethacin; D010634:Phenobarbital