8-Substituted guanosine and 2'-deoxyguanosine derivatives as potential inducers of the differentiation of Friend erythroleukemia cells.
A variety of 8-substituted guanosine and 2'-deoxyguanosine derivatives were synthesized and tested as inducers of the differentiation of Friend murine erythroleukemia cells in culture. The most active agents in the guanosine series were 8-substituted-N(CH3)2, -NHCH3, -NH2, -OH, and -SO2CH3, which caused 68, 42, 34, 33, and 30% of erythroleukemia cells to attain benzidine positivity, a functional measure of maturation, at concentrations of 5, 1, 0.4, 5, and 5 mM, respectively. The 8-OH derivative of the 2'-deoxyguanosine series produced comparable activity, causing 62% benzidine-positive cells at a level of 0.2 mM. These findings indicate that 8-substituted analogues of guanosine and 2'-deoxyguanosine have the potential to terminate leukemia cell proliferation through conversion to end-stage differentiated cells.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005622:Friend murine leukemia virus; D006151:Guanosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000502:physiology; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D006151:Guanosine; C046215:8-hydroxyguanosine; C032533:8-aminoguanosine; C035169:8-bromoguanosine; D007041:Hypoxanthine Phosphoribosyltransferase; D003849:Deoxyguanosine