Primary structure of the soybean nodulin-23 gene and potential regulatory elements in the 5'-flanking regions of nodulin and leghemoglobin genes.
The nodulin-23 gene of soybean is one of the most abundantly transcribed genes induced during symbiosis with Rhizobium. Using a plasmid (pNod25) from a nodule cDNA library, we have isolated the nodulin-23 gene from a soybean genomic library. Nucleotide sequence analysis of the cDNA and of the genomic clone indicated that the coding region of this gene is 669 bp long and is interrupted by a single intron of about 530 bp. The deduced protein sequence suggests that nodulin-23 may have a signal sequence. The 5'-flanking sequence of two other nodulin genes, nodulin-24 encoding for a membrane polypeptide and one of the leghemoglobin genes (LbC3), were obtained. Comparison of these sequences revealed three conserved regions, one of which, an octanucleotide (GTTTCCCT), has 100% homology. The conserved sequences are arranged in a unique fashion and have a spatial organization with respect to order and position, which may suggest a potential regulatory role in controlling the expression of nodulin and leghemoglobin genes during symbiosis.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D006420:Hemeproteins / Q000235:genetics*; D007874:Leghemoglobin / Q000235:genetics*; D008565:Membrane Proteins; D009876:Operon; D010940:Plant Proteins / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D013025:Glycine max / Q000235:genetics*; D014158:Transcription, Genetic
D006420:Hemeproteins; D007874:Leghemoglobin; D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin; D004247:DNA