Activation of the c-K-ras oncogene in a human pancreas carcinoma.
The human pancreas carcinoma cell line T3M-4 contains activated c-Kirsten (K)-ras oncogene detectable by the DNA-mediated gene transfer technique using NIH/3T3 cells. DNA fragments containing coding lesions have been cloned, and nucleotide sequence analysis suggests that the T3M-4 oncogene has been activated by a single nucleotide transition from A to C in the second exon, which results in the substitution of histidine for glutamine in coden 61 of the predicted amino acid sequence. The quantity analysis of c-K-ras oncogene in the DNA and RNA of T3M-4 cells revealed that the c-K-ras gene was amplified and overexpressed in T3M-4 cells. These findings indicate that the T3M-4 c-K-ras oncogene is activated by different mutational events.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000032:analysis; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005784:Gene Amplification; D006801:Humans; D008297:Male; D009857:Oncogenes; D010190:Pancreatic Neoplasms / Q000235:genetics*
D004247:DNA