Electrochemical luminescence-based homogeneous immunoassay.
Aromatic hydrocarbon such as pyrene capable of generating electrochemical luminescence was employed as a label of immunoassay. Pyrene labeled antigen generated luminescence upon electrolytic reduction, while the luminescence decreased remarkably in the presence of antibody. The labeled antigen (constant) and free antigen were competitively reacted to the constant amount of antibody. The luminescence was correlated to the antigen concentration as little as 10(-6)M antigen. The proposed method is a very unique immunochemical technique which requires no BF separation.
D000906:Antibodies / Q000276:immunology; D004563:Electrochemistry; D006801:Humans; D007158:Immunologic Techniques; D008163:Luminescent Measurements; D008722:Methods; D011721:Pyrenes; D012709:Serum Albumin / Q000276:immunology
D000906:Antibodies; D011721:Pyrenes; D012709:Serum Albumin; C030984:pyrene