The human leucocyte P30 antigen: properties and assessment of value as a clonal marker.
FMC3 and FMC29 are monoclonal antibodies which react with a 30,000 Dalton molecule expressed on the lymphocyte surface membrane. The molecule is a protein which does not appear to be N-glycosylated. The antigen, which is also expressed intracellularly, does not appear to be a marker of differentiation or maturation. Polyclonal cell populations, such as peripheral blood lymphocytes, show a bimodal distribution of surface antigen density, whilst monoclonal cell populations analysed quantitatively showed unimodal antigen density distributions. This suggests the antigen may be a clonal marker.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification; D002454:Cell Differentiation / Q000187:drug effects; D002999:Clone Cells / Q000276:immunology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D007938:Leukemia / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008970:Molecular Weight; D010641:Phenotype; D010703:Phorbol Esters / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D010703:Phorbol Esters; D004121:Dimethyl Sulfoxide