Spectrofluorometric determination of bacterial DNA base composition.
A spectrofluorometric technique for bacterial DNA base composition has been developed. This fast and simple technique requires two fluorescent dyes and a few inexpensive reagents. The data from this assay indicate that the guanine-cytosine content obtained was within acceptable statistical limits in comparison to commonly cited literature values. The spectrofluorometric technique is reliable and reproducible.
D001482:Base Composition; D055598:Chemical Phenomena; D002621:Chemistry; D003013:Clostridium / Q000235:genetics; D003596:Cytosine / Q000032:analysis*; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D006147:Guanine / Q000032:analysis*; D008837:Micrococcus / Q000235:genetics; D013050:Spectrometry, Fluorescence; D014733:Vibrio / Q000235:genetics
D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine