2-5A-dependent endoribonuclease activity and effects of A2'p5'A2'p5'A (2-5A core) in mouse pancreatic islets.
Glucose-stimulated (pro)insulin biosynthesis is markedly inhibited is mouse pancreatic islets incubated with micromolar concentrations of 2-5A 'core', (A2'p5'A2'p5'A). Total protein synthesis was also reduced, but to a lesser extent, while insulin release and total insulin content of the islets was untouched by 2-5A 'core'. Evidence is given for the presence of a 2-5A-dependent endoribonuclease which mediates these effects.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D004722:Endoribonucleases / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009841:Oligonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000494:pharmacology*; D011070:Poly I-C / Q000494:pharmacology; D011384:Proinsulin / Q000096:biosynthesis
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D011384:Proinsulin; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D005947:Glucose; D011070:Poly I-C