title: Monoclonal antibodies which distinguish certain classes of neuronal and supporting cells in the nervous tissue of the nematode Caenorhabditis elegans.
abstract: Monoclonal antibodies were generated using mice immunized with total homogenates of Caenorhabditis elegans adults or early larvae. Two of them were shown to distinguish a certain class of neuronal or supporting cells in the nervous tissue of this animal. Their histological specificities were studied in detail by indirect immunofluorescence on a whole mount preparation of animal head (or tail); for one of the antibodies further analysis was done by immunoelectron microscopy with the aid of a colloidal gold probe. An application of this antibody to a mutant of C. elegans is also described.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D009154:Mutation; D009348:Nematoda / Q000276:immunology*; D009417:Nerve Tissue / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D009421:Nervous System Malformations; D009474:Neurons / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal