Cell lineage evaluation in preimplantation mouse embryos by intracellular injection of IgG.
The cell lineage in preimplantation mouse embryos was studied by injecting rabbit IgG into zygotes or into a blastomere of early or late 2-cell, 3- or 4-cell stage embryos. The localization of the IgG was detected by the peroxidase-antiperoxidase method. It was found that starting from the late 2-cell stage, lineage could be followed up to the cell populations of the blastocyst, inner cell mass and trophectoderm.
D000818:Animals; D001757:Blastomeres; D002454:Cell Differentiation; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D005260:Female; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D051379:Mice; D011247:Pregnancy; D015053:Zygote
D007074:Immunoglobulin G