title: Platelet sensitivity in vitro to adenosine-5'-diphosphate and prostacyclin and diabetic retinopathy.
abstract: Platelet sensitivity to adenosine diphosphate and prostacyclin in diabetes has been assessed using collision theory and the concept of 'sticking probability' (the probability of particle union). Twenty Type 1 (insulin-dependent) diabetic men (10 with no or minimal retinopathy and a matched group of 10 with proliferative retinopathy) and 10 age-matched nondiabetic men were studied. Platelets from the 20 diabetic patients required, on average, 37% less adenosine diphosphate to achieve a sticking probability of 0.5 (ED50) compared with platelets from the non-diabetic subjects (medians 1.50 and 0.95 mumol/l, respectively; p less than 0.01). The platelet prostacyclin response was assessed by the dissociation constant (Ki) for inhibition of adenosine diphosphate-induced aggregation. Platelets from the diabetic patients had similar prostacyclin sensitivity to those from the non-diabetic subjects (medians 0.42 and 0.42 respectively). Diabetic patients with and without retinopathy had similar platelet sensitivity to both adenosine diphosphate and prostacyclin.
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000367:Age Factors; D001786:Blood Glucose / Q000032:analysis; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D003930:Diabetic Retinopathy / Q000097:blood*; D011464:Epoprostenol / Q000494:pharmacology*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008954:Models, Biological; D010974:Platelet Aggregation / Q000187:drug effects*
chemical_list: D000244:Adenosine Diphosphate; D001786:Blood Glucose; D011464:Epoprostenol; D006442:Glycated Hemoglobin