Immunoperoxidase assay for the detection of specific IgG antibodies to Hantaan virus.
A technique, using indirect immunoperoxidase antibody (IPA), was developed for the detection of IgG antibody to Hantaan virus. The same protein A-peroxidase conjugate was used with mouse, rat and human sera. The IPA technique employs glass slides with air-dried gamma-ray-inactivated and acetone-fixed Hantaan-infected Vero-E6 cells. Antibody titers detected by IPA was comparable to those detected by the indirect fluorescent antibody technique.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002043:Bunyaviridae / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D051379:Mice; D051381:Rats
D000914:Antibodies, Viral; D007074:Immunoglobulin G