Characterization of host lymphoid cells in antibody-facilitated bone marrow chimeras.
We have produced stable murine antibody-facilitated (AF) chimeras by the simultaneous injection of P1 bone marrow cells and anti-P2 monoclonal antibody into normal (unirradiated) adult (P1 X P2)F1 recipients. These AF chimeras are healthy, long-lived, and exhibit no overt signs of graft-versus-host disease. They are immunocompetent and tolerant of host, P2-encoded alloantigens. Donor cell engraftment and takeover, monitored by glucosephosphate isomerase isozyme patterns, is usually complete (greater than 95%) in the peripheral blood, bone marrow, and hemopoietic stem cell compartments of long-term (greater than 3 months posttransplantation) AF chimeras. We report here, however, that splenic, lymph node, and thymic leukocytes of AF chimeras represent donor/host chimeric populations. Spleen cell populations of AF chimeras exhibit substantial chimera-to-chimera variation in the preponderant residual host cell type(s) present. Our interpretations of the implications of these findings are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D007108:Immune Tolerance; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000145:classification / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal