Efficient purification of somatomedin-C/insulin-like growth factor I using immunoaffinity chromatography.
Somatomedin-C/insulin-like growth factor I was purified from human plasma using a monoclonal antibody affinity column. Combining immunoaffinity chromatography with standard protein purification methods resulted in an overall recovery of 18%. The 35 micrograms of somatomedin-C/insulin-like growth factor I purified from 500 ml of plasma appeared as a single band when analyzed by polyacrylamide gel electrophoresis and could be used in radioimmunoassay and receptor binding studies.
D000328:Adult; D002846:Chromatography, Affinity / Q000379:methods; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007328:Insulin / Q000302:isolation & purification*; D007334:Insulin-Like Growth Factor I; D007525:Isoelectric Focusing; D010455:Peptides / Q000302:isolation & purification*; D013002:Somatomedins / Q000302:isolation & purification*
D007328:Insulin; D010455:Peptides; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I