A method of purifying sheep sIg+ lymphocytes as a tool for class II MHC antigen analysis.
A method is described for the purification of sheep lymphocytes carrying class II MHC antigens. After incubation of purified blood lymphocytes on anti-IgM-coated petri dishes, the adherent fraction contained 95% sIg-positive cells determined by immunofluorescence. When tested with cross-reacting anti-class II (bovine and human) monoclonal antibodies, more than 95% of these cells were positive either by immunofluorescence or cytotoxicity. This technique will permit studies of the polymorphism of sheep class II antigens.
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation / Q000379:methods; D006041:Goats / Q000276:immunology; D006649:Histocompatibility Antigens / Q000032:analysis*; D007962:Leukocytes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D012756:Sheep / Q000276:immunology*; D013045:Species Specificity; D013552:Swine / Q000276:immunology
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D011947:Receptors, Antigen, B-Cell