Structure of apoproteins in insect lipophorin.
The two polypeptide chains of cockroach and locust lipophorins were separated and their amino acid compositions were determined. Circular dichroic spectra of the lipophorins and apolipophorin from 190 to 250 nm showed a single trough at 218 nm and a peak at 194 nm. Infrared spectra of the lipophorins in D2O showed a strong peak at 1625 cm-1 and a weak shoulder at 1693 cm-1 corresponding to v (pi, 0) and nu (0, pi) of antiparallel pleated sheet. The resonance frequency splitting delta nu = nu (0, pi) -nu (pi, 0) was 68 cm-1, which was larger than that of ordinary globular proteins containing antiparallel pleated sheet. From circular dichroic and infrared spectra it was concluded that lipophorins contained polypeptides rich in antiparallel pleated sheet with longer unbroken extensions than the case for ordinary globular proteins. Partial proteolytic digestion study of lipophorins with trypsin, chymotrypsin, and subtilisin showed that the larger apolipophorin (AL1) was exposed to the surface of the particle and the smaller apolipophorin (AL2) lay protected from the attack of the enzymes. Crosslinked products between AL1 and AL2 were readily obtained when dimethylsuberimidate or dimethyladipimidate was added to the lipophorin solution, without giving lipophorin dimers, suggesting that the two chains were located within 11 A from each other. Such structural features of insect lipoprotein were compared with other insect lipophorins and the human serum low-density lipoprotein (LDL). Similarities between lipophorins and LDL were found in the molecular weight, amino acid compositions, and the secondary structure of major apoproteins.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001059:Apoproteins / Q000032:analysis*; D002352:Carrier Proteins / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D003058:Cockroaches; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006110:Grasshoppers; D008074:Lipoproteins; D010447:Peptide Hydrolases; D013055:Spectrophotometry, Infrared
D000596:Amino Acids; D001059:Apoproteins; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D008074:Lipoproteins; C032830:lipophorin; D010447:Peptide Hydrolases