Monoclonal antibody production to a B16 melanoma associated antigen.
Eight different rat hybridoma cell lines have been produced which secrete MAbs identifying the melanoma-specific B700 antigen. This antigen has been previously shown to have significant sequence homology to the mammalian serum albumins, but antibodies specifically raised against B700 cross-react only with murine albumin. Each of these MAbs has been shown by Western immunoblotting to recognize an independent epitope on the B700 protein; it is concluded not only that the B700 protein is a highly immunogenic antigen, but also that the sequence homologies between the B700 antigen and murine albumin must occur extensively throughout the molecule.
D000418:Albumins / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000302:isolation & purification; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D008545:Melanoma / Q000276:immunology*; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods
D000418:Albumins; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D010446:Peptide Fragments