Liquid nitrogen storage of cultured T lymphocytes.
The conditions required for storing and recovering IL-2-dependent T cell clones from liquid nitrogen were investigated. For maximum cell recovery, cultured T lymphocytes were precooled at 4 degrees C for 15 min in medium containing 10% DMSO and 20% FCS before storage in liquid nitrogen. This method allowed adequate time for DMSO to penetrate the cells before freezing.
D002470:Cell Survival; D005615:Freezing; D006801:Humans; D011309:Preservation, Biological; D013601:T-Lymphocytes