title: Carbohydrates detected by lectins in the vestibular organ.
abstract: In the vestibular organ the presence of carbohydrates in the cupula and otoconia of young (6-day-old) and adult rats was investigated using fluorescent lectins. The following sugars have been identified in both young and adult rats: N-acetyl-glucosamine, galactose, mannose and fucose. In contrast, N-acetyl-galactosamine was not detected. In order to demonstrate the specificity of the reaction, control experiments were performed after preincubation of the lectin with its specific inhibitory sugar. The same sugars were identified in calcified (i.e. otoconia) and non-calcified (i.e. cupula) structures. The role of these sugars in the mineralization and fusion process of otoconia is discussed.
mesh_terms: D000818:Animals; D003053:Cochlear Duct / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D037102:Lectins; D008358:Mannose / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D010037:Otolithic Membrane / Q000378:metabolism; D051381:Rats; D014722:Vestibule, Labyrinth / Q000378:metabolism*
chemical_list: D037102:Lectins; D009844:Oligosaccharides; D005643:Fucose; D005944:Glucosamine; D008358:Mannose; D005690:Galactose