B lymphocyte regulation of human hematopoiesis.
Epstein Barr virus (EBV)-transformed B lymphoblastoid cell lines (BLCL) were derived from seven different individuals. The ability of BLCL supernatants to stimulate hematopoietic colony formation in vitro was tested in a conventional stem cell assay system. Supernatants promoted the growth of single (GM, E, MK) as well as multi-lineage (GEMM) colonies in bone marrow cultures. Our results indicate that EBV-transformed B lymphocytes produce cytokines that affect in vitro stem cell proliferation and differentiation. These studies demonstrate the regulatory potential of activated B lymphocytes in human hematopoiesis.
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D002474:Cell-Free System; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis / Q000502:physiology; D003470:Culture Media; D004306:Dose-Response Relationship, Immunologic; D006410:Hematopoiesis; D006801:Humans; D008213:Lymphocyte Activation; D009174:Mycoplasma / Q000378:metabolism
D003115:Colony-Stimulating Factors; D003470:Culture Media