title: "Thiocyanate gold": small (2-3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy.
abstract: Reduction of HAuCl4 by NaSCN or KSCN produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation approximately 15%). The AuSCN sol forms protein-gold complexes. The amount of protein required to form an AuSCN-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates. By immuno-electron microscopy SCN-gold complexed to protein A is active and visible as is shown by revealing alpha-amylase in rat pancreatic acinar cells.
mesh_terms: D000818:Animals; D003102:Colloids; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D008297:Male; D008854:Microscopy, Electron; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013205:Staphylococcal Protein A; D013861:Thiocyanates; D000516:alpha-Amylases / Q000032:analysis
chemical_list: D003102:Colloids; D013205:Staphylococcal Protein A; D013861:Thiocyanates; D006046:Gold; D000516:alpha-Amylases; C031760:thiocyanate