Growth-activity related variation of a nuclear antigen in monolayer-culture cells quantitated by immunofluorometry and microspectrophotometry.
Antibodies of the hybridoma clone M 372/809 react with a nuclear antigen in growing cells. Using immunofluorometry and microspectrophotometry, the antigen was found to accumulate to a maximum coincident with the marked early increase in nuclear protein contents. The following DNA increase appeared when the intensity of the reaction with M 372/809 antibodies was decreasing. The antigen increase was inhibited by alpha-amanitin. It may therefore be associated with transcription early in the cell cycle.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D034961:Antigens, Nuclear; D002455:Cell Division; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D005455:Fluorescent Antibody Technique; D006801:Humans; D008856:Microscopy, Fluorescence; D009698:Nucleoproteins / Q000032:analysis* / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
D000546:Amanitins; D000911:Antibodies, Monoclonal; D000941:Antigens; D034961:Antigens, Nuclear; D009698:Nucleoproteins; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin