A comparison of methods for enumerating lymphocyte classes.
We have evaluated a novel kit method for enumerating B and T lymphocytes using antibody-coupled beads. The technique proved to be an unsuitable alternative to sheep red blood cell rosettes and immunofluorescence. However, the principle is potentially valuable and we suggest certain modifications which would make the method more suitable for routine use.
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000145:classification; D005455:Fluorescent Antibody Technique; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D008214:Lymphocytes / Q000145:classification*; D008863:Microspheres; D011933:Reagent Kits, Diagnostic; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic