title: Effect of reduced temperature on glycoprotein (Ig, HLA) processing and transport in lymphoid cells.
abstract: Secretion of Igs and surface expression of HLA antigens was examined in lymphoid cells as a function of temp. Upon reducing the temp from 37 to 20 degrees C a progressive decrease in the secretion of Ig and surface expression of HLA antigens was noted. When the status of the oligosaccharides present on these glycoproteins was examined, conversion of high-mannose [endo-beta-N-acetylglucosaminidase-(Endo H) sensitive] to complex-type (Endo H resistant) oligosaccharides diminished with decreasing temp. At no time was an accumulation of Endo H resistant glycoproteins seen intracellularly. These results show that the phenomenon observed for synthesis and intracellular transport of viral glycoproteins in epithelial cells at reduced temp, namely intracellular accumulation of viral glycoproteins carrying complex sugar moieties, does not necessarily apply to glycoprotein transport in lymphoid cells. A difference in subcellular organization of epithelial and lymphoid cells may be responsible for this discrepancy.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001692:Biological Transport; D002460:Cell Line; D003080:Cold Temperature; D006023:Glycoproteins / Q000378:metabolism*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007072:Immunoglobulin D / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D051379:Mice
chemical_list: D000954:Antigens, Surface; D006023:Glycoproteins; D006680:HLA Antigens; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D007136:Immunoglobulins