The production and characterization of monoclonal antibodies to the human thyroid microsome.
By suitable immunization of mice and fusion of their spleen cells with a non-secretor mouse myeloma line, monoclonal antibodies have been produced which react with the human thyroid microsomal (M) antigen. These monoclonal antibodies showed no reactivity by enzyme-linked immunoassay with liver microsomes or thyroglobulin and their specificity was confirmed by immunolocalization studies, in which they showed the staining characteristics of human M antibodies. All four monoclonal antibodies tested were immunoglobulin M; three were cytotoxic to thyroid cell monolayers. The lack of cytotoxicity with the fourth monoclonal supports the concept that certain epitopes of the M antigen may be partially or completely absent at the thyroid cell surface. These monoclonal antibodies should permit further characterization of the thyroid M antigen in view of their absence of cross-reactivity with thyroglobulin.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008861:Microsomes / Q000276:immunology*; D013961:Thyroid Gland / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M