[Comparative evaluation of the efficacy of methods of cryo- and trypsin activation of inactive renin in vitro].
The total renin activity (TRP) and inactive renin levels (IR) in the blood plasma were examined with the help of cryo- and trypsin activation in 9 normal subjects, in 40 patients with essential hypertension and in 18 patients with primary aldosteronism (PA). The cryoactivation method was shown to detect 40-80% of IR determined by the method of trypsin activation. Both methods revealed analogous ratios of the two renin forms in its total production in the presence of essential hypertension (in different "renin" subgroups) and arterial hypertension secondary to PA and may be used as the methods of choice when examining essential hypertension patients with the normal and subnormal plasma renin activity. The method of trypsin activation is more preferable for a more accurate quantification of small amounts of IR found in essential hypertension patients with a high plasma renin activity. With a high level of the TRP (over 8 ng/ml/h) activation should be performed in diluted plasma.
D000328:Adult; D003080:Cold Temperature; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000097:blood*; D006801:Humans; D006929:Hyperaldosteronism / Q000097:blood*; D006973:Hypertension / Q000097:blood*; D066298:In Vitro Techniques; D012083:Renin / Q000097:blood* / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D004792:Enzyme Precursors; D014357:Trypsin; D012083:Renin
Sravnitel'naia otsenka éffektivnosti metodov krio- i tripsinovoĭ aktivatsii neaktivnogo renina in vitro.