Functional preservation of the mammalian kidney. VI. Viability assessment of rabbit kidneys perfused at 25 degrees C with dimethyl sulfoxide in RPS-2.
The study utilizes an ex vivo perfusion model to evaluate the effects of warm perfusion (25 degrees C) with two K+-rich vehicle solutions (RPS-2 and solution A) on whole rabbit kidneys. The suitability of the solutions as vehicles for introducing cryoprotectant concentrations (2.8 and 3.5 M) of dimethyl sulfoxide (Me2SO) and the effects of the addition of albumin to RPS-2 are also tested. Albumin (5 g/dl) does not appreciably improve the renal perfusion dynamics of RPS-2. Results indicate that both RPS-2 and solution A serve as effective vehicles for the introduction of 2.8 M Me2SO, and the continued investigation of the RPS-2/Me2SO protocol is advocated.
D000042:Absorption; D000818:Animals; D003080:Cold Temperature; D004121:Dimethyl Sulfoxide; D005919:Glomerular Filtration Rate; D005947:Glucose / Q000378:metabolism; D007668:Kidney / Q000502:physiology*; D016030:Kidney Transplantation; D009926:Organ Preservation / Q000379:methods*; D009929:Organ Size; D010477:Perfusion; D011188:Potassium / Q000378:metabolism; D011817:Rabbits; D012079:Renal Circulation; D012996:Solutions
D012996:Solutions; D005947:Glucose; D011188:Potassium; D004121:Dimethyl Sulfoxide