Generation of antigen specific cytotoxic T lymphocytes (CTL) directed against class II molecules expressed by activated T cells.
Mitogen-activated T cells were used to provide a source of Class II antigens to CTL originally stimulated against mononuclear cells expressing both foreign Class I and Class II determinants. Our results indicated that 7-10-day-old activated T antigen-presenting cells, which shared only Class II antigens with the original priming cell, were able to stimulate the differentiation of CTL-recognizing Class II determinants. The use of 14-day-old activated T cells as target cells in the CML assay, compared with 72 h PHA blasts or 7-day-old activated T cells, enabled a more sensitive detection of the anti-Class II CTL.
D000938:Antigen-Presenting Cells / Q000276:immunology; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*
D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II; D010835:Phytohemagglutinins