Acute phase serodiagnosis using Quik-Sep ion exchange chromatography.
A commercial ion-exchange column method (Quik-Sep) for isolation of immunoglobulin M (IgM) free from immunoglobulin G (IgG) was evaluated. After column separation, serum IgM recovery averaged 88% (46-100%) with IgG removal averaging 95% (91-100%). Rubella and Toxoplasma gondii IgM antibodies were recovered without a change in titer, whereas IgG antibodies were removed effectively. Rheumatoid factor (RF) interference and IgG blocking antibodies were eliminated following column chromatography.
D000208:Acute Disease / Q000175:diagnosis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D012409:Rubella / Q000175:diagnosis; D012412:Rubella virus / Q000276:immunology; D012698:Serologic Tests / Q000379:methods; D014122:Toxoplasma / Q000276:immunology
D007074:Immunoglobulin G; D007075:Immunoglobulin M