title: Dual cutaneous reactions to anti-human IgE; no change in plasma level of the split product complement C3d or effect of heparin.
abstract: The possible dependence of the dual cutaneous reaction (DCR) on activation of the complement and coagulation systems was further elucidated. Heterologous whole anti-human IgE was injected intradermally (i.d.) on the volar aspect of the forearms of 17 drug-free healthy volunteers and venous samples for the selected laboratory test collected from the adjacent cubital vein. Plasma level of C3 split products (C3d) did not change significantly 6 h and 96 h after the injection of anti-IgE inducing immediate cutaneous reactions (ICRs) or DCRs as compared with prechallenge values. Conventional heparin, a compound with anti-coagulant and anti-complementary effects, injected i.d. (200 IE) concomitantly with and by s.c. (1000 IE) infiltration of the whealing 15 and 90 min subsequently to anti-IgE challenge produced no significant inhibition of the resulting ICRs or DCRs. The results suggest that the previously reported involvement of the coagulation process is a secondary phenomenon and do not support the concept that activation of the complement system is necessary for the evolution of DCRs.
mesh_terms: D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D003176:Complement C3 / Q000097:blood; D015933:Complement C3d; D003165:Complement System Proteins / Q000502:physiology*; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D006967:Hypersensitivity / Q000503:physiopathology*; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D012882:Skin Tests; D013997:Time Factors
chemical_list: D000888:Antibodies, Anti-Idiotypic; D003176:Complement C3; D007073:Immunoglobulin E; D015933:Complement C3d; D006493:Heparin; D003165:Complement System Proteins