Elimination of the allatotropic activity in locusts by microsurgical and immunological methods: evidence for humoral control of the corpora allata, hemolymph proteins, and ovary development.
Transection of the nervous connections (NCC I) between the brain and the corpus cardiacum (group 1) and injection of anti-brain antibodies inhibited vitellogenin synthesis and ovary development in virgin Locusta migratoria females. Removal of the neurosecretory material by these methods changed the titer and the electrophoretic pattern of hemolymph proteins, including vitellogenin. Transection of NCC I at some distance from the brain resulted in bulbs with 30-200 micron diameter which were filled with stainable neurosecretory material (group 2). Oocyte maturation was delayed in group 2 but normal eggs were laid. The same happened if group 1 animals received daily injections of JH-III. It is concluded that a humoral factor is involved in the activation of JH-III biosynthesis. The factor is transported through the NCC I and is released into the hemolymph as allatotropin.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001798:Blood Proteins / Q000378:metabolism / Q000502:physiology*; D001921:Brain / Q000276:immunology; D002369:Castration; D003335:Corpora Allata / Q000502:physiology*; D005260:Female; D006110:Grasshoppers / Q000254:growth & development / Q000502:physiology*; D006458:Hemolymph / Q000378:metabolism / Q000502:physiology*; D007158:Immunologic Techniques; D008866:Microsurgery; D010053:Ovary / Q000254:growth & development*; D014819:Vitellogenins / Q000378:metabolism
D000906:Antibodies; D001798:Blood Proteins; D014819:Vitellogenins