Error analysis in titration microcalorimetry of biochemical systems.
A simplified method for titrations of biochemical systems is described as well as extensive error propagation through the data analysis. This work uses a Tronac Model 450 isoperibol titration calorimeter. Sample volumes of 2 ml are used and total heats of less than 5 mcal can be routinely measured. The binding of 3'-CMP to bovine pancreatic ribonuclease A is used to illustrate the methods. The binding enthalpy can be determined with a standard deviation of 1.5% and the free energy with a standard deviation of 2 to 3%.
D000818:Animals; D002151:Calorimetry / Q000379:methods* / Q000592:standards; D002417:Cattle; D003568:Cytidine Monophosphate / Q000378:metabolism; D007201:Indicator Dilution Techniques; D008832:Microchemistry; D011485:Protein Binding; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D013816:Thermodynamics
D012259:Ribonuclease, Pancreatic; D003568:Cytidine Monophosphate