Transformation and growth related changes in levels of nuclear and cytoplasmic proteins antigenically related to mammalian beta-galactoside-binding lectin.
Immunofluorescence and immunoblotting experiments, using a monoclonal antibody to the 13 kDa mammalian beta-galactoside-binding lectin have shown that human lymphocytes contain nuclear and cytoplasmic proteins of apparent molecular masses of 130, 80, 65 and 13 kDa that are antigenically related to the lectin and whose levels and patterns of expression change in association with transformation, or after stimulation with mitogens. These observations, together with the finding that the myeloid cell line K562 is also rich in the 130 kDa component, whereas the mature granulocytes of normal donors and of patients with chronic myeloid leukaemia are lacking in all of the immunoreactive forms, raise the possibility that this family of lectin-related proteins may be components of growth regulatory systems that are variously elicited in the transformed and stimulated cells.
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D001345:Autoradiography; D001798:Blood Proteins / Q000032:analysis*; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D037161:Galectins; D006098:Granulocytes / Q000378:metabolism*; D006388:Hemagglutinins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007120:Immunochemistry; D007938:Leukemia / Q000097:blood / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D009698:Nucleoproteins / Q000097:blood*
D000911:Antibodies, Monoclonal; D000941:Antigens; D001798:Blood Proteins; D037161:Galectins; D006388:Hemagglutinins; D009698:Nucleoproteins