7-ethoxycoumarin deethylase activity as indicator of oxidative metabolism in rabbit eyes.
Oxidative drug metabolism in intraocular tissues (choroid, cornea, iris-ciliary body and retina) plus conjunctiva and liver was determined in rabbits. 7-Ethoxycoumarin deethylase activity was found in all the intraocular tissues, but enzyme activities in these tissues were fairly low compared to activities in the conjunctiva and liver. Iris-ciliary body showed the highest enzyme activity and cornea showed the lowest. Relatively high activities were found in conjunctiva: 21 and 120 times as high as those in iris-ciliary body of young and adult rabbit eyes, respectively. The administration of phenobarbital or 3-methylcholanthrene (3-MC) to young rabbits caused marked induction of enzyme activity only in choroid tissues. Phenobarbital, but not 3-MC, also induced enzyme activities in conjunctiva and liver.
D015660:7-Alkoxycoumarin O-Dealkylase; D000818:Animals; D003374:Coumarins / Q000378:metabolism*; D004790:Enzyme Induction; D005123:Eye / Q000201:enzymology*; D005260:Female; D008658:Inactivation, Metabolic; D010105:Oxygenases / Q000502:physiology*; D011817:Rabbits
D003374:Coumarins; C017299:7-ethoxycoumarin; D010105:Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase