Preparation of antibodies to guinea pig IgE and its use for enzyme-linked immunosorbent assay of IgE antibodies.
Guinea pigs were infected with Trichinella spiralis. A pooled serum from the infected guinea pigs was fractionated by DEAE-cellulose column chromatography and Sephadex G-200 gel filtration. An IgE-rich fraction was injected into rabbits. The antiserum from the rabbits, after appropriate absorption with a normal guinea pig plasma, formed a precipitin line in immunoelectrophoresis and in immunodiffusion against a guinea pig serum containing IgE antibodies to ovalbumin. Uptake of 125I ovalbumin was observed in radioimmunoelectrophoresis. This anti IgE could be used in enzyme-linked immunosorbent assay of IgE antibodies to ovalbumin.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D007158:Immunologic Techniques; D010047:Ovalbumin / Q000276:immunology; D014235:Trichinellosis / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007073:Immunoglobulin E; D010047:Ovalbumin