title: Isolation of the epidermal growth factor from the shrew submaxillary gland.
abstract: The epidermal growth factor (EGF) from the submaxillary glands of the Chinese Shrew (Suncus murinus) is purified to apparent homogeneity by using a sequence of four chromatographic steps, viz. gel filtration on Sephacryl S-200, affinity chromatography on immobilized Ni, hydrophobic interaction on phenyl-Sepharose CL-4B and reverse-phase HPLC. An 800-fold increase in specific activity and an overall recovery of 46% were achieved. The most effective step in its purification is the successful use of immobilized metal ion affinity chromatography (IMAC). This method was very selective, reproducible and requires a minimum of sample pre-treatment prior to chromatography.
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D004815:Epidermal Growth Factor / Q000302:isolation & purification*; D012788:Shrews / Q000378:metabolism*; D013363:Submandibular Gland / Q000032:analysis*
chemical_list: D004815:Epidermal Growth Factor