[Primary structure of 20S,22R-cholesterol-hydroxylating cytochrome P-450 from bovine adrenal cortex mitochondria. Study of the structure of peptides obtained by hydrolysis of fragment F1 with proteinase from Staphylococcus aureus].
The fragment F1 resulting from the limited tryptic hydrolysis of the native molecule of cytochrome P-450 has been digested with Staphylococcus aureus protease. 24 peptides, covering the whole polypeptide chain of fragment F1, are isolated from the hydrolysate. Analysis of their amino acid sequence in combination with the earlier data on the structure of cytochrome P-450 chymotryptic peptides and fragment F1 tryptic peptides permitted to carry out a reconstruction of large peptide blocks of fragment F1 that comprise 252 amino acid residues.
D000302:Adrenal Cortex / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D010450:Endopeptidases; D006868:Hydrolysis; D066298:In Vitro Techniques; D008299:Maleic Anhydrides; D008666:Metalloendopeptidases; D008928:Mitochondria / Q000201:enzymology*; D010088:Oxidoreductases / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis
D008299:Maleic Anhydrides; D010446:Peptide Fragments; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D002786:Cholesterol Side-Chain Cleavage Enzyme; D010450:Endopeptidases; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus
Pervichnaia struktura 20S,22R-kholesteringidroksiliruiushchego tsitokhroma P-450 iz mitokhondriĭ nadpochechnikov byka. II. Issledovanie struktury peptidov, poluchennykh pri gidrolize fragmenta F1 proteinazoĭ iz Staphylococcus aureus.