title: Characterisation of a cell line from a human lung lymphosarcoma. Abundance of vimentin-type intermediate filaments.
abstract: A cell line (HCL82) was established from a human lung lymphosarcoma. Cells exhibited a stable phenotype and remained nearly diploid with a modal chromosome number of 45-48, showing no significant aberrations. Cultures consisted of single elongated cells and irregular colonies. Ultrastructurally, mitochondria, golgi complexes, and large bundles of intermediate filaments were well developed; some cells contained virus-like particles. Filaments were composed of vimentin identified by immunofluorescence and gel electrophoresis. Reaction with monoclonal antibody OKT4 suggested a close relationship to T-helper cells. Cells did grow at clonal density but did not form tumors in nude mice.
mesh_terms: D000818:Animals; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D014746:Vimentin / Q000378:metabolism
chemical_list: D014746:Vimentin