A simultaneous assay for T-cells and B-cells using Immunobeads.
A comparison was made between a new, commercially available light microscope assay for T- and B-lymphocyte enumeration and existing procedures. The Quantigen assay employs two color-coded Immunobeads; one Immunobead binds to and labels T-cells, while the second labels B-cells. Phagocytic cells contaminating lymphocyte preparations ingest both types of beads. A high degree of correlation was found between this simultaneous assay for T- and B-cells and the E-rosette method for T-cells and FITC-labeling of B-cell surface immunoglobulin. The results of a comparison of the Quantigen assay to flow cytometric analyses are also given. Data on chronic lymphocytic leukemia samples are presented, demonstrating that these cells are double-markers with the Quantigen assay because of the use of monoclonal antibody T101, which recognizes T-cells and B-CLL cells.
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007163:Immunosorbent Techniques; D007945:Leukemia, Lymphoid / Q000276:immunology; D007958:Leukocyte Count / Q000379:methods*; D008856:Microscopy, Fluorescence; D009000:Monocytes / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc