title: Influence of a dorsomedial hypothalamus lesion on the circadian changes in the enzyme development and activity of intestinal brushborder membranes in the rat.
abstract: Circadian changes in activities and electrophoretic pattern of the intestinal brushborder enzymes alkaline phosphatase (EC 3.1.3.1), isomaltase (EC 3.2.1.10) and sucrase (EC 3.2.1.26) of normal rats and of rats with a lesion of the dorsomedial hypothalamus (DMH-rats) were studied. In contrast to the rhythm found in normal rats, there are no significant differences between the average enzyme activities in the light or dark period for the DMH-rats. The peak ratio in the electrophoretic pattern for sucrase-isomaltase (SI) over pro-sucrase-isomaltase (pro-SI) changes over 24 hr with higher values during the active (dark) period of the normal rat. In DMH-rats this ratio is much higher, but no significant difference could be demonstrated between the values near the acrophase in these rats.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002940:Circadian Rhythm; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007033:Hypothalamus, Middle / Q000502:physiology*; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure; D007583:Jejunum / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000201:enzymology; D009834:Oligo-1,6-Glucosidase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000032:analysis; D013393:Sucrase / Q000378:metabolism*
chemical_list: D000469:Alkaline Phosphatase; D006026:Glycoside Hydrolases; D009834:Oligo-1,6-Glucosidase; D013393:Sucrase