MicroELISA method for measurement of human serum thymosin alpha 1.
A microELISA for the estimation of human serum thymosin alpha 1 is described. In this assay, antibody to thymosin alpha 1 is pre-incubated with the standard or serum at 4 degrees C. Unbound antibody in the liquid-phase then binds with solid-phase thymosin alpha 1. The method is sufficiently sensitive for measuring serum levels of thymosin alpha 1 and highly reproducible. The serum levels measured with the microELISA are comparable to serum levels of thymosin alpha 1 determined by the previously described radioimmunoassay for thymosin alpha 1.
D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D008832:Microchemistry; D011863:Radioimmunoassay; D013696:Temperature; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000097:blood
D013947:Thymosin; D000077596:Thymalfasin