Stabilisation of human interferon beta synthesis in Escherichia coli by zinc ions.
Human interferon beta synthesized in Escherichia coli is unstable and toxic for the bacterial cell. Zinc ions are able to stabilise interferon beta in E. coli probably by inhibiting the action of cell internal proteinase(s) which affect the half-life of this foreign protein. As a result up to one order of magnitude more active IFN-beta can be detected in Zn2+-treated E. coli cells.
D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005470:Fluorometry; D005786:Gene Expression Regulation; D006207:Half-Life; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D015032:Zinc / Q000494:pharmacology*
D007370:Interferon Type I; D015032:Zinc