Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.
Monoclonal antibodies have been used to characterize molecules found on the surfaces of T cells of sheep. SBU-T4 and STU-T8 are present on 80-85% of thymocytes, absent from B lymphocytes, and present on two-thirds and one-third of lymph node T lymphocytes, respectively. Double-labelling of T lymphocytes shows that the populations recognized are mutually exclusive. Immunoprecipitation of the SBU-T4 antigen from thymocytes and T lymphocytes reveals a molecule which migrates on SDS-PAGE as a single band of 56,000 MW under both reducing and non-reducing conditions. Under reducing conditions, the SBU-T8 molecule migrates as two bands of 33,000 and 36,000 MW when immunoprecipitated from thymocytes, but only the higher MW band is present following immunoprecipitation from peripheral lymphocytes. Under non-reducing conditions, SBU-T8 migrates as dimers and other multiples. Based on immunofluorescent, immunochemical and immunohistological data, these molecules are considered to be the structural analogues of the marker molecules used to delineate T-helper and T-cytotoxic/suppressor lymphocyte subsets of other species.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008221:Lymphoid Tissue / Q000276:immunology; D011863:Radioimmunoassay; D012756:Sheep / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification* / Q000187:drug effects / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D014357:Trypsin