L-Threonine dehydrogenase from goat liver. Feedback inhibition by methylglyoxal.
L-Threonine dehydrogenase, which forms aminoacetone from L-threonine and NAD, has been extensively purified from goat liver. A feedback inhibition of this enzyme has been observed with methylglyoxal. Kinetic data and other experiments indicate that methylglyoxal acts at a site other than the active site of the enzyme. The enzyme contains a single subunit of Mr 89,000. The apparent Km values of the enzyme for L-threonine and NAD were found to be 5.5 and 1 mM, respectively.
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D000494:Allosteric Regulation; D000818:Animals; D006041:Goats; D008099:Liver / Q000201:enzymology*; D011765:Pyruvaldehyde / Q000494:pharmacology
D011765:Pyruvaldehyde; D000429:Alcohol Oxidoreductases; C023159:L-threonine 3-dehydrogenase