Determination of [3H]TdR-labelling indices of cultured cells grown on detachable chamber slides.
The in vitro effects of xenobiotic agents on the incorporation of DNA precursors was determined using a detachable chamber slide assembly. The technique presented allows for the comparative assessment of several agents, even when a limited number of cells is available. The use of a glass slide also potentiates the use of higher magnifications with a greater resultant resolution than that achievable with past methods.
D001345:Autoradiography / Q000379:methods*; D002478:Cells, Cultured; D006652:Histological Techniques / Q000295:instrumentation; D013936:Thymidine / Q000032:analysis; D014316:Tritium
D014316:Tritium; D013936:Thymidine