title: Platelet tropomyosin binding protein is identical with serum albumin.
abstract: We have shown that the platelet tropomyosin binding protein described in the accompanying paper (Gerhard, M. D., DiGirolamo, P. M., and Hitchcock-DeGregori, S. E. (1985) J. Biol. Chem. 260, 3221-3227) is identical with human serum albumin. The immunological determinants are completely shared; the tryptic peptide maps are the same; the proteins comigrate on two-dimensional gels; and the amino acid sequences of the first 33 amino acids are the same. Although human serum albumin in plasma or commercially prepared protein will not bind tropomyosin-Affi-Gel 15, it will bind following purification from plasma by chromatography on hydroxylapatite.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D002352:Carrier Proteins / Q000097:blood*; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits; D012709:Serum Albumin / Q000032:analysis*; D014335:Tropomyosin / Q000097:blood*; D014357:Trypsin / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D010446:Peptide Fragments; D012709:Serum Albumin; D014335:Tropomyosin; D014357:Trypsin