1,25-Dihydroxyvitamin D3 and 1,24-dihydroxyvitamin D3 suppress in vitro antibody response to T cell-dependent antigen.
1,25-Dihydroxyvitamin D3 and 1,24-dihydroxyvitamin D3 suppressed an antibody response to sheep red blood cells (SRBC, T cell-dependent antigen) by murine splenocytes, in concentrations ranging from 10(-10)-10(-7)M. These suppressive effects were markedly abrogated when T cell-depleted lymphocytes were cultured in the presence of a supernatant of concanavalin A-stimulated spleen cells. On the contrary, neither of them suppressed antibody response to trinitrophenyl-lipopolysaccharide (T cell-independent antigen). These results suggest that the suppressive effect of active vitamin D3 on anti-SRBC response was mediated by the inhibition of T cells.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D000955:Antigens, T-Independent / Q000276:immunology; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004100:Dihydroxycholecalciferols / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D000955:Antigens, T-Independent; D004100:Dihydroxycholecalciferols; D003208:Concanavalin A; C023850:1 alpha,24-dihydroxyvitamin D3; D002117:Calcitriol