title: Interleukin 1 can act as a B-cell growth and differentiation factor.
abstract: Splenic B lymphocytes specifically reactive to the hapten fluorescein (FLU) were prepared from nonimmune adult mice by affinity fractionation on hapten-gelatin. These FLU-specific B cells were cultured as single cells or in small numbers in 10-microliter wells either in the absence of any feeder, filler, or accessory cell or in the presence of 3T3 fibroblasts acting as filler cells. A selected batch of a "T-cell-independent" antigen, FLU-Ficoll, which induces growth and differentiation only in the presence of lymphokines or cytokines acting as B-cell growth and differentiation factors (BGDF), was used as the antigenic stimulus. It was found that murine interleukin 1 prepared by recombinant DNA technology was an effective, although weak, BGDF when acting with antigen on B cells cultured either under filler cell-free conditions or in the presence of 3T3 cells. When the murine interleukin 1 was used in combination with recombinant human interleukin 2, itself a weak but effective BGDF in the system, an additive effect was observed. The results challenge the notion that interleukin 1 is exclusively or even primarily an activating cytokine. This system, in which pure factors are able to act with specific antigen on single hapten-specific B cells, will prove helpful for the further dissection of the respective roles of the various factors that can act on B cells.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001681:Biological Assay; D002454:Cell Differentiation; D002455:Cell Division; D002999:Clone Cells / Q000276:immunology; D006133:Growth Substances / Q000276:immunology*; D007375:Interleukin-1 / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000276:immunology; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology*
chemical_list: D000941:Antigens; D006133:Growth Substances; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D011994:Recombinant Proteins; D015847:Interleukin-4