title: Cl transport across the basolateral membrane in frog skin epithelium.
abstract: Cellular Cl concentrations were determined by electron microprobe analysis to obtain further insight into the Cl transport across the basolateral membrane of the frog skin epithelium. Cl-free media on the serosal side led in all epithelial layers within 1 h to a decrease in cellular Cl concentration from about 40 to 15 mmol/kg wet wt, whereas the application of Cl-free solutions or amiloride to the apical side had no effect. Na-free media, furosemide or bumetanide on the serosal side had little effect on cellular Cl but abolished the Cl-reuptake into Cl-depleted cells. It is concluded that cellular Cl concentration is maintained above electrochemical equilibrium by a co-transport system, which is relatively silent under control conditions.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002034:Bumetanide / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005665:Furosemide / Q000494:pharmacology; D007700:Kinetics; D011896:Rana temporaria; D012867:Skin / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D014882:Water-Electrolyte Balance / Q000187:drug effects
chemical_list: D002712:Chlorides; D002034:Bumetanide; D005665:Furosemide; D012964:Sodium