Requirements for in vitro reconstruction of protein synthesis.
Translation of f2am3 RNA and MS2 RNA in a system containing purified ribosomes and precharged aminoacyl-tRNA, does not occur in the presence of the "known" initiation (IF-1, IF-2 and IF-3), elongation (EF-Tu, EF-Ts and EF-G) and termination (RF-1, RF-2) factors. Translation in this system requires at least three additional protein factors. These are EF-P and the rescue protein as well as a previously undescribed factor we call W, which is found in the high-salt ribosomal eluate.
D001426:Bacterial Proteins / Q000096:biosynthesis; D002384:Catalysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010445:Peptide Elongation Factors / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012358:RNA, Transfer, Met; D012270:Ribosomes / Q000378:metabolism
D001426:Bacterial Proteins; D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-