Androgen receptor content in cytosol from non-tumoral human kidney and its relation to steroid hormone environment.
The content of cytoplasmic androgen receptors (Bmax) was analyzed in non-tumoral renal tissue of 12 men and 15 postmenopausal women using a synthetic androgen (methyltrienolone) as ligand and a method of dextran-coated charcoal. The Bmax in both sexes was compared, establishing correlations between it and circulating levels of testosterone, dehydroepiandrosterone sulfate, androstenedione, and estradiol to find out the possible influence of the hormonal environment on androgen receptors in the human kidney. No differences in Bmax were observed between males (46.3 +/- 24 fmol/g of tissue) and females (45.4 +/- 26 fmol/g), in spite of the significantly greater (p less than 0.01) levels of circulating testosterone in the former group. No significant linear correlations existed between any of the steroids analyzed and the Bmax. These results demonstrate the existence of androgenic receptors in non-tumoral human kidney and indicate that its content is not regulated by circulating levels of testosterone. The concentrations of the principal extratesticular androgens and estradiol do not seem to have a quantitative influence on these androphilic proteins either.
D000328:Adult; D000368:Aged; D000728:Androgens / Q000097:blood / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D004963:Estrenes / Q000378:metabolism; D004967:Estrogens / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D008297:Male; D015741:Metribolone; D008875:Middle Aged; D010502:Perineum / Q000378:metabolism; D011467:Prostate / Q000378:metabolism; D011869:Radioligand Assay; D011944:Receptors, Androgen / Q000378:metabolism* / Q000502:physiology; D013737:Testis / Q000378:metabolism; D045165:Testosterone Congeners / Q000378:metabolism
D000728:Androgens; D004963:Estrenes; D004967:Estrogens; D011944:Receptors, Androgen; D045165:Testosterone Congeners; D015741:Metribolone