title: Effects of host serum on feeding by Trichostrongylus colubriformis (nematoda).
abstract: The effect of host serum on in vitro feeding by Trichostrongylus colubriformis was studied by incubating adult helminths in goat serum containing the dye, Rhodamine B. The amount of dye ingested was determined by fluorometric analysis. Immune serum from goats infected with T. colubriformis suppressed helminth feeding, while normal serum from uninfected goats did not. Suppression of feeding by immune serum increased with the duration of the host's infection. Heat-inactivation (56 degrees C) of immune serum did not affect its suppressive activity. Pre-exposure of worms to immune serum decreased subsequent feeding activity. However, rigorous washing of helminths restored their feeding to levels that were similar to untreated worms. Indirect immunofluorescent studies with immune serum and FITC conjugated rabbit anti-goat IgG demonstrated binding of immunoglobulin to the cuticle, stoma and excretory pore of whole worms. Feeding inhibition of immune serum was associated with IgG1 isotype. Results of the present studies indicated that IgG was responsible for in vitro suppression of T. colubriformis feeding and may be one effector of immunity to T. colubriformis in the goat.
mesh_terms: D000818:Animals; D005247:Feeding Behavior; D005260:Female; D005455:Fluorescent Antibody Technique; D006041:Goats; D006358:Hot Temperature; D007106:Immune Sera / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D014251:Trichostrongyloidea / Q000276:immunology / Q000502:physiology*; D014252:Trichostrongyloidiasis / Q000276:immunology*; D014253:Trichostrongylosis / Q000276:immunology* / Q000469:parasitology
chemical_list: D007106:Immune Sera; D007074:Immunoglobulin G