Vesicles and cisternae in the trans Golgi apparatus of human fibroblasts are acidic compartments.
Recently we demonstrated that low-pH compartments can be visualized with the electron microscope using a basic congener of dinitrophenol, 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine (DAMP), which concentrates in acidic compartments and can be detected by immunocytochemistry with a monoclonal anti-dinitrophenol antibody. We now report that DAMP also accumulates in cisternae and vesicles associated with the trans face of the Golgi apparatus. DAMP rapidly leaves this compartment when cells are incubated with the ionophore monensin, which indicates that accumulation is due to the acidic pH in this compartment. Using indirect protein A-gold immunocytochemistry, we localized fibronectin, a major secretory protein in fibroblasts, to the trans Golgi vesicles that took up DAMP. Therefore, the trans cisternae of the Golgi apparatus and forming secretory vesicles have an acidic pH.
D000818:Animals; D004136:Dinitrobenzenes / Q000378:metabolism; D005347:Fibroblasts / Q000648:ultrastructure*; D005353:Fibronectins / Q000378:metabolism; D006046:Gold; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure*; D006651:Histocytochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D037102:Lectins; D051379:Mice; D008854:Microscopy, Electron; D014909:Wheat Germ Agglutinins
D004136:Dinitrobenzenes; D005353:Fibronectins; D037102:Lectins; D014909:Wheat Germ Agglutinins; D006046:Gold; C042432:3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine