title: Site-specific analysis of drug interactions and damage in DNA using sequencing techniques.
abstract: DNA-sequencing techniques can be adapted to provide powerful analytical tools for pinpointing the sites at which DNA is modified by either radiations or chemicals. Base modifications, covalent adducts, crosslinks, and noncovalent binding can all be detected. This review outlines the adaptions of the Maxam-Gilbert and Sanger dideoxy sequencing techniques which make such studies possible. Practical aspects and limitations of the various methods are given. Assays which test the ability of DNA polymerase to bypass damage and misincorporate bases are also discussed. It is concluded that these techniques constitute the most powerful method currently available for the study of sequence-specific DNA interactions.
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000494:pharmacology; D006801:Humans; D007457:Iodine Radioisotopes; D008722:Methods; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D014176:Protein Biosynthesis
chemical_list: D007457:Iodine Radioisotopes; D004364:Pharmaceutical Preparations; D010761:Phosphorus Radioisotopes; D004247:DNA; D004259:DNA-Directed DNA Polymerase