title: Effect of dexamethasone on serum protein extravasation in experimental brain infarcts of monkey: an immunohistochemical study.
abstract: Experimental brain infarcts were produced in 12 adult baboons (Papio cynocephalus) by transorbital permanent clipping of the left middle cerebral artery. One group (seven monkeys) received daily injections of 1 mg/kg dexamethasone, starting 1 h after vascular occlusion and continuing till the end of the experiment. Another group (five monkeys) was not treated. One week after vascular occlusion the volume of infarcts and peri-infarct edema was estimated morphometrically on histological sections, using Masson's trichrome stain and the peroxidase-anti-peroxidase (PAP) technique for visualization of serum protein extravasation. In the untreated animals the average volume of infarct was 6.57 +/- 4.23% (mean +/- SD) and the volume of edema 7.83 +/- 2.93% of ipsilateral hemisphere. In the treated animals the infarct volume was not different (7.95 +/- 3.00%), but the volume of peri-infarct edema was significantly lower (2.82 +/- 3.06%, p less than 0.05). The results obtained indicate that dexamethasone treatment reduces the development of peri-infarct edema but does not influence the size of infarcts.
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001812:Blood-Brain Barrier; D001929:Brain Edema / Q000209:etiology / Q000517:prevention & control*; D002544:Cerebral Infarction / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism; D003907:Dexamethasone / Q000627:therapeutic use*; D005260:Female; D007124:Immunoenzyme Techniques; D008297:Male; D010215:Papio
chemical_list: D001798:Blood Proteins; D003907:Dexamethasone