Inhibition of prolipoprotein signal peptidase by globomycin.
Globomycin inhibits the prolipoprotein-specific signal peptidase activity by binding to the enzyme in a noncompetitive manner (Ki = 36 nM). The Km of prolipoprotein signal peptidase for the prolipoprotein substrate is 6 (+/- 1) microM.
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001665:Binding Sites; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D007700:Kinetics; D008074:Lipoproteins; D008565:Membrane Proteins; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D011480:Protease Inhibitors; D011498:Protein Precursors / Q000378:metabolism; D012697:Serine Endopeptidases
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C099932:Lpp protein, E coli; D008565:Membrane Proteins; D010455:Peptides; D011480:Protease Inhibitors; D011498:Protein Precursors; C017339:globomycin; D012697:Serine Endopeptidases; C026515:type I signal peptidase