Interferon-like activity in an anti-interferon anti-idiotypic hybridoma antibody.
We describe here an anti-idiotypic hybridoma antibody directed against affinity-purified rabbit idiotypic antibodies (Rb-Id) to a homogeneous protein, the recombinant human leukocyte A interferon (rIFN-alpha A). The supernatant of the hybridoma, designated 3-1B, was able to inhibit the neutralization of rIFN-alpha A activity by the idiotypic antibodies. An in vivo passage of uncloned 3-1B cells yielded hybridoma cells (presumably a subclone), designated 3B1, the supernatant of which exhibited interferon-like antiviral activity with both bovine kidney (MDBK) cells and human amnion (WISH) cells. This activity could be absorbed by polymer-bound goat anti-mouse immunoglobulin serum and by Rb-Id coupled to Affi-Gel 10, and could be partially eluted from the latter at pH 2.5. The anti-idiotypic hybridoma antibody was able to compete with 125I-rIFN-alpha A for binding to the Rb-Id and also to interferon receptor-bearing MDBK cells. The clinical significance of an interferon-like anti-idiotypic antibody is discussed.
D000042:Absorption; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001667:Binding, Competitive; D002417:Cattle; D002460:Cell Line; D005260:Female; D006041:Goats / Q000276:immunology; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D007130:Immunoglobulin Idiotypes / Q000276:immunology* / Q000378:metabolism; D007370:Interferon Type I / Q000276:immunology* / Q000378:metabolism; D007668:Kidney; D051379:Mice; D009500:Neutralization Tests / Q000379:methods; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007370:Interferon Type I