Stabilization of the adenylate energy charge by the depletion of adenylates without glycolytic stimulation.
A relationship between the AMP deaminase reaction and the recovery of the energy charge was examined using permeabilized yeast cells. Citrate inhibited the glycolytic flux and the recovery of the energy charge. The addition of spermine enhanced the recovery of the energy charge without the reversal of the inhibition of glycolysis in the presence of excess citrate. The AMP deaminase reaction can participate in the stabilization of the energy charge only by the depletion of total adenylates, and not by the glycolytic stimulation under the conditions where citrate is highly increased during aerobic growth conditions.
D000659:AMP Deaminase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D006019:Glycolysis; D010539:Permeability; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D012441:Saccharomyces cerevisiae; D013096:Spermine / Q000494:pharmacology
D000227:Adenine Nucleotides; D002951:Citrates; D011773:Pyruvates; D019343:Citric Acid; D013096:Spermine; D000641:Ammonia; D019289:Pyruvic Acid; D000659:AMP Deaminase