title: Synthesis of Escherichia coli carbamoylphosphate synthetase initiates at a UUG codon.
abstract: The ribosome binding region of the messenger RNA for the Escherichia coli carA gene contains two adjacent putative translational start codons, UUG and AUU, both of them unusual. By Edman degradation and mass spectrometry of purified carA protein, we show that only UUG is used in vivo. Translation initiation at UUG in carA appears about half as efficient as at AUG in lacZ.
mesh_terms: D000595:Amino Acid Sequence; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000096:biosynthesis / Q000235:genetics*; D003062:Codon / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008401:Gas Chromatography-Mass Spectrometry; D008025:Ligases / Q000235:genetics*; D010442:Peptide Chain Initiation, Translational; D010446:Peptide Fragments / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D003062:Codon; D010446:Peptide Fragments; D012333:RNA, Messenger; D008025:Ligases; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)