All-reagent test tablets and method for rapid and selective alpha-amylase iodometric determination.
The new type of test tablets (Iodocrom) for alpha-amylase assay contain crosslinked (CL)-amylose or CL-starch (specific substrates for alpha-amylase only) and the reagent (KIO3/KI) generating iodine (in acidic medium, when the reaction is stopped). The method--amyloclastic in nature--is based on selective action of alpha-amylase on the CL-substrate liberating soluble polysaccharide chains large enough and in a conformation suitable to allow the formation of iodine inclusion complexes. Unlike the classical iodometric methods, the reaction is followed by an increase in iodine complex blue color. The method has some common points with the well-known chromogenic (e.g., Phadebas) methods. Both use insoluble substrates which are not susceptible to attack by exoamylases and in both cases the enzymatic reaction is followed by the release of soluble products. The amounts of these released chains and the absorbances of their inclusion complexes with iodine are in a linear dependence with the enzyme concentration (activity).
D006801:Humans; D007452:Iodates; D011188:Potassium; D017680:Potassium Compounds; D011193:Potassium Iodide; D011933:Reagent Kits, Diagnostic; D012463:Saliva / Q000201:enzymology; D000516:alpha-Amylases / Q000032:analysis*
D007452:Iodates; D017680:Potassium Compounds; D011933:Reagent Kits, Diagnostic; D011193:Potassium Iodide; D000516:alpha-Amylases; C039693:potassium iodate; D011188:Potassium