Suppression of B-cell and T-cell responses by the prostaglandin-induced T-cell-derived suppressors (PITS)--III. Production of PITS beta factors from T-cell hybridomas.
Previous studies have shown that the prostaglandin-induced T-cell-derived suppressors (PITS) are actually a mixture of at least seven distinct factors. These factors may be reproducibly resolved by size-exclusion chromatography followed by high-pressure liquid chromatography (HPLC). Results are presented here which suggest that two T-lymphocyte hybridoma clones are capable of constitutive synthesis of three of these suppressor factors. Results show that clone Hyb-7SC2 produces factors which co-elute (by HPLC) with the PITS beta2 and PITS beta3 factors, while clone Hyb-9SC2 produces a factor which co-elutes with PITS beta1. These results suggest the possibility that the seven PITS beta factors are not the product of a single cell population.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D006825:Hybridomas / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis* / Q000494:pharmacology; D051379:Mice; D008938:Mitosis; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000378:metabolism*
D008222:Lymphokines; D013491:Suppressor Factors, Immunologic