Semi-synthetic preparation of 1-O-[1'-14C]hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) using plant cell cultures.
Incubation of photomixotrophic cell suspension cultures of rape (Brassica napus) and heterotrophic cell suspension cultures of soya (Glycine max) with 1-O-[1'-14C]hexadecyl-sn-glycerol or rac-1-O-[1'-14C]hexadecylglycerol leads in high yield (up to 78%) to labeled 1-O-hexadecyl-2-acyl-sn-glycero-3-phosphocholines. Alkaline hydrolysis of the choline glycerophospholipids yields pure 1-O-[1'-14C]hexadecyl-sn-glycero-3-phosphocholine. 1-O-[1'-14C]Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) is obtained by acetylating the lyso compound. The semi-synthetic preparation described leads to labeled platelet activating factor in an overall yield of 50-60% without loss of specific activity.
D001937:Brassica; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D007202:Indicators and Reagents; D019308:Palmitic Acid; D010169:Palmitic Acids; D010944:Plants / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000138:chemical synthesis* / Q000302:isolation & purification; D011865:Radioisotope Dilution Technique; D013025:Glycine max
D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D010169:Palmitic Acids; D010972:Platelet Activating Factor; D019308:Palmitic Acid