title: Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae.
abstract: We present genetic evidence for the enzymes 4-aminobutyrate: 2-oxoglutarate aminotransferase (EC 2.6.1.19) and succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16) constituting the functional pathway for the utilization of 4-aminobutyric acid as a nitrogen source by Saccharomyces cerevisiae. We show that the pathway is induced by 4-aminobutyric acid and that the presence of the pathway enzymes probably requires the integrity of a positive control element.
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000235:genetics / Q000302:isolation & purification; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000302:isolation & purification; D002463:Cell Membrane Permeability; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D050644:Succinate-Semialdehyde Dehydrogenase; D050604:Succinate-Semialdehyde Dehydrogenase (NADP+); D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D005680:gamma-Aminobutyric Acid; D000445:Aldehyde Oxidoreductases; D050604:Succinate-Semialdehyde Dehydrogenase (NADP+); C493646:UGA2 protein, S cerevisiae; D050644:Succinate-Semialdehyde Dehydrogenase; D000612:4-Aminobutyrate Transaminase; D009584:Nitrogen