Predictive value of assessment of lymph node weight and T-lymphocyte proliferation in contact sensitivity in acrylates.
An assessment of T-lymphocyte proliferation and lymph node weight is proposed as a predictive test for contact sensitizers of industrial origin. Data are presented showing increased T-lymphocyte proliferation following epicutaneous application of a variety of industrially important acrylate-like chemicals which appear to correlate well with their ability to sensitize in the guinea pig. These data were compared with those obtained after application of 2,4-dinitro-1-fluorobenzene (DNFB) a strong sensitizer, and 2,4-dinitrothiocyanatebenzene (DNTB) a nonsensitizer when given epicutaneously. It is suggested that this quantitative approach, in parallel with a simple one-dose immunization, may provide a better picture of sensitization potential than the longer multidose immunizations currently in use.
D000179:Acrylates / Q000008:administration & dosage / Q000494:pharmacology*; D000287:Administration, Topical; D000818:Animals; D003877:Dermatitis, Contact / Q000175:diagnosis / Q000209:etiology* / Q000276:immunology; D004136:Dinitrobenzenes / Q000008:administration & dosage / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D005260:Female; D006168:Guinea Pigs; D008198:Lymph Nodes / Q000033:anatomy & histology*; D008213:Lymphocyte Activation; D008297:Male; D009929:Organ Size; D013601:T-Lymphocytes / Q000276:immunology*
D000179:Acrylates; D004136:Dinitrobenzenes