Low colloid osmotic pressure improves liver preservation in the model of the isolated perfused guinea pig liver.
In order to study the effect of two perfusion solutes with a different oncotic level on the preservation of the isolated liver, we perfused 14 guinea pig livers with a modified KREBS-HENSELEIT buffer with either 3%- or 6% bovine albumin. The release of four selected plasma proteins, standard transaminases, potassium and urea and the degree of hepatic oxygen consumption were evaluated. The results show a superiority of the 3%-solution with improvement of the synthetic capacity for albumin and fibrinogen and, in contrast to the 6%-solution, a stable potassium level and hepatic oxygen consumption.
D000818:Animals; D003102:Colloids; D005260:Female; D005340:Fibrinogen / Q000096:biosynthesis; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008954:Models, Biological; D009926:Organ Preservation / Q000379:methods*; D009997:Osmotic Pressure; D010101:Oxygen Consumption; D010477:Perfusion; D011188:Potassium / Q000378:metabolism; D012709:Serum Albumin / Q000096:biosynthesis
D003102:Colloids; D012709:Serum Albumin; D005340:Fibrinogen; D011188:Potassium