Inhibition of experimental autoimmune uveoretinitis in rats by S-antigen-specific monoclonal antibodies.
Mouse monoclonal antibodies (mAb), of either IgG2a or IgG2b isotypes, specific for the retinal S-autoantigen (S-Ag) or a pool of rat anti-S-Ag sera prevented experimental autoimmune uveoretinitis in Lewis rats when injected i.p. at the time of immunization. Control mAb of the same isotypes, irrelevant to S-Ag, had no inhibitory effect. The humoral response to S-Ag, as studied by enzyme-linked immunosorbent assay using a mouse mAb specific for rat kappa chain, was moderately but significantly reduced in suppressed animals. The rapid disappearance of the injected mAb from rat sera, as measured using a rat mAb specific for mouse kappa chain, could be explained by its complexing with either autologous antigen released from the retina at the site of inflammation, or anti-idiotypic antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000032:analysis / Q000096:biosynthesis; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D019393:Arrestin; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D007106:Immune Sera / Q000032:analysis; D007108:Immune Tolerance; D007274:Injections, Intraperitoneal; D051381:Rats; D011917:Rats, Inbred Lew; D012173:Retinitis / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D014605:Uveitis / Q000209:etiology / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D007106:Immune Sera