Response of a phage modification factor to enhanced production of its target molecule.
Escherichia coli cells with plasmids bearing the valS gene were constructed from the Clark-Carbon collection. Their increased valyl-tRNA synthetase (EC 6.1.1.9) activity was not matched by commensurate production of a modifying peptide during T4 infection. Thus, phage vs gene expression is set to modify the normal amount of valyl-tRNA synthetase activity.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D013604:T-Phages / Q000254:growth & development / Q000378:metabolism*; D014637:Valine-tRNA Ligase / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D014764:Viral Proteins; D000604:Amino Acyl-tRNA Synthetases; D014637:Valine-tRNA Ligase