[Role of the cytoskeleton in the morphological normalization of transformed cells in culture].
The role of the cytoskeleton in morphological normalization of transformed cells was studied. Mouse cells of the L197/6 clonal line were fused by polyethylene glycol and replated. The multinuclear cells were more spread than control ones: the ratio of the cell-occupied area to the number of the nuclei increased 2-3 times as a result of multinucleation. Instead of the spindle-like morphology typical for control cells they became star-like with larger lamellar regions located between radially oriented cell processes. According to the immunofluorescent data these processes contained thick bundles of microtubules and intermediate filaments. Destruction of these bundles with colcemide led to a decrease in the area occupied by multinuclear cells but did not change significantly the area occupied by control cells. The role of microtubules and intermediate filaments in cell spreading is discussed.
D000818:Animals; D002459:Cell Fusion; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000648:ultrastructure*; D002999:Clone Cells / Q000648:ultrastructure; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D003703:Demecolcine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007739:L Cells / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure
D003703:Demecolcine
Rol' tsitoskeleta v morfologicheskoĭ normalizatsii transformirovannkh kletok v kul'ture.