Enhanced interleukin 1 (IL-1) production mediated by mouse serum amyloid P component.
Purified serum amyloid P component (SAP), the major acute-phase reactant of mice, induces enhanced interleukin 1 (IL-1) production by elicited monocytes/macrophages in vitro. SAP also enhanced IL-1 elaboration by macrophages from lipopolysaccharide (LPS)-low responder mice and in the presence of polymyxin B, indicating that the small amounts of LPS present in the SAP preparation did not augment IL-1 production. Concentrations of SAP of 0.1 to 10.0 micrograms/ml enhanced IL-1 production by elicited and bacillus Calmette-Guerin (BCG)-activated peritoneal macrophages, but not by resident peritoneal macrophages. The inflammation-induced monocyte/macrophage population displayed selective binding of SAP. The mouse macrophage line P388D1, also could bind SAP and display enhanced IL-1 production in response to SAP. SAP did not bind to the macrophage cell line RAW264.7 nor did it enhance IL-1 secretion by this line. The results suggest that this acute-phase reactant has the potential to enhance inflammatory and immunological events mediated by IL-1.
D000682:Amyloid / Q000097:blood* / Q000494:pharmacology; D000818:Animals; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D008264:Macrophages / Q000145:classification / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D000683:Serum Amyloid P-Component
D000682:Amyloid; D007375:Interleukin-1; D000683:Serum Amyloid P-Component