title: Renin gene expression in vessels of the ovine renal cortex.
abstract: Using hybridization histochemistry, a technique which localizes specific mRNA populations in tissue sections with a 700 base pair recombinant DNA probe which codes for ovine renin, we have localized renin gene expression in the afferent arteriole of the juxtaglomerular apparatus (JGA) in the sheep renal cortex. Specific labelling representing renin gene expression was also found at a distance from the glomerular tuft in the walls of the afferent arteriole and also in cells in the medial layer of larger vessels of the renal cortex, specifically the interlobular arteries. These observations provide morphological evidence of renin gene expression at these sites and, combined with ultrastructural and immunocytochemical evidence suggest that renin is synthesized and stored in the afferent arteriole either within the JGA or at a distance from the glomerulus, and in the smooth muscle coat of the interlobular arteries in the sheep kidney.
mesh_terms: D000818:Animals; D001160:Arterioles / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000032:analysis; D004274:DNA, Recombinant; D007606:Juxtaglomerular Apparatus / Q000098:blood supply / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D012077:Renal Artery / Q000378:metabolism*; D012083:Renin / Q000096:biosynthesis* / Q000235:genetics; D012756:Sheep
chemical_list: D004274:DNA, Recombinant; D012083:Renin