title: Non-specific cross-reacting antigen (NCA) in individual maturation stages of myelocytic cell series.
abstract: The distribution and localization of NCA and carcinoembryonic antigen CEA in cells of different types of myelogenous leukaemias (acute myelogenous leukaemia - AML; chronic granulocytic leukaemia - CGL; CGL in myeloblastic crisis - CGL-BC) was studied using the immunofluorescence test. Discontinuous density-gradient centrifugation was used to separate myeloid cells into fractions containing granulocytes in individual stages of maturation. Serum NCA and CEA levels were estimated in parallel. It was established that: (a) AML blasts without maturation (MO type) and monoblasts did not synthesize NCA; (b) individual blasts of AML with features of maturation (M1, M2 types) and some myeloblasts of CGL-BC exhibited a limited ability to express cytoplasmic NCA; (c) the number of NCA-containing cells increased in the more mature granulocyte fractions isolated on Ficoll-Hypaque density-gradients; (d) myelocytic NCA is immunologically related to NCA isolated from lung tissue and (e) CEA is undetectable in the myelocytic cell series.
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D015815:Cell Adhesion Molecules; D002454:Cell Differentiation; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology*; D015470:Leukemia, Myeloid, Acute / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D006023:Glycoproteins