Growth of the Escherichia coli cell envelope.
The growth pattern of the Escherichia coli envelope was studied by immunoelectron microscopy, using the outer membrane protein LamB specifically labelled by a double antibody gold particle technique. An operon fusion placing the lamB gene under lac promoter control permitted rapid turn-off of LamB synthesis. In the generation following turn-off no lamB-free regions appeared, strongly suggesting that bulk outer membrane material is not inserted in restricted growth zones.
D001425:Bacterial Outer Membrane Proteins; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D004926:Escherichia coli / Q000254:growth & development*; D006046:Gold; D007158:Immunologic Techniques; D007763:Lac Operon; D008854:Microscopy, Electron; D018272:Porins; D011991:Receptors, Virus / Q000096:biosynthesis / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D018272:Porins; D011991:Receptors, Virus; C016532:maltoporins; D006046:Gold