Chloroquine removal of HLA antigens from platelets for the platelet immunofluorescence test.
Chloroquine-treated platelets did not react with HLA antisera in a platelet suspension immunofluorescence test (PSIFT), while the reactions with platelet-specific antibodies (P1A1, Baka) seemed even stronger after treatment. The method might be useful in discerning HLA and platelet-specific antibodies without absorption procedures. The treatment causes death of a proportion of the cells, with nonspecific fluorescence making the reading difficult. This may be circumvented by using special phase-contrast lenses.
D000906:Antibodies / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology*; D002738:Chloroquine / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans
D000906:Antibodies; D006680:HLA Antigens; D002738:Chloroquine