Antibodies to rheumatoid arthritis nuclear antigen (RANA) in Japanese patients with rheumatoid arthritis.
We studied antibodies to rheumatoid arthritis nuclear antigen (RANA) by the Ouchterlony method in 0.5% agarose plates, using soluble antigen extracted with 0.25 M sucrose solution from cultured Raji cells. Anti-RANA antibody was found in sera from 24 to 40 (60%) patients with rheumatoid arthritis (RA), from 4 of 20 (20%) patients with systemic lupus erythematosus (SLE), and from 2 of 30 (7%) healthy controls. When sucrose extracts from BJAB, Ramos, and JM cells were used as the cellular antigens, no anti-RANA precipitin lines were detected. Indirect immunofluorescence study, using Raji cells or human B lymphocytes transformed by EB virus as substrate tissues, demonstrated anti-RANA antibody as fine speckled nuclear staining. Although RA patients with positive anti-RANA antibody usually had high titers of anti-Epstein-Barr nuclear antigen (EBNA) and anti-viral capsid antigen (VCA) IgG antibodies, the Wilcoxon ranks sum test showed no close statistical correlation between the presence of anti-RANA antibodies and the titers of anti-EBNA or anti-VCA (IgG) antibodies. These data showed that the incidence of positivity of anti-RANA antibodies in Japanese RA patients is almost the same as that in American and European RA patients.
D000328:Adult; D000906:Antibodies / Q000032:analysis*; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000941:Antigens / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000503:physiopathology; D001324:Autoantigens / Q000276:immunology*; D002213:Capsid / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000503:physiopathology; D008875:Middle Aged
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D000956:Antigens, Viral; D001324:Autoantigens; D019309:Epstein-Barr Virus Nuclear Antigens; D007074:Immunoglobulin G; C030352:rheumatoid arthritis nuclear antigen