title: Expression in Escherichia coli of a fusion protein product containing a region of the adenovirus DNA polymerase.
abstract: The bulk of an open reading frame extending from map coordinates 23.3 to 14.2 in region E2b of the adenoviral genome has been cloned and expressed from a chimeric plasmid in Escherichia coli. The cloning strategy used created a fusion protein of 124,000 daltons, which contained greater than 98% adenovirus-encoded sequences. Antiserum raised against this protein reacted with the authentic 140,000-dalton adenovirus DNA polymerase. Another serum raised against a synthetic hexapeptide whose sequence corresponded to the predicted carboxyl terminus of adenovirus-encoded DNA polymerase also reacted with the fusion protein and authentic adenovirus DNA polymerase. These results demonstrate that the cloned region of DNA encodes the adenovirus DNA polymerase.
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D007120:Immunochemistry; D010957:Plasmids; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase