title: [Production of monoclonal antibodies].
abstract: Monoclonal antibodies are prepared by a process of somatic cell hybridization. During this process the antibody-forming plasma cells are fused with myeloma cells. Hybrid cells continue to proliferate and to secrete antibodies. Homogeneous cell population which secrete a monoclonal product are obtained by cloning of the hybrid cells. The reactivity of the monoclonal antiserum is then determined by a screening procedure (for example by immunoassay). The principles of the production and the screening of monoclonal antibodies are presented.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007136:Immunoglobulins
vernacular_title: Produktie van monoclonale antilichamen.