Beta-lactamase stability of temocillin.
The stability of temocillin to 12 different beta-lactamase preparations was studied using high pressure liquid chromatography and was compared with that of aztreonam, cefotetan and cefotaxime. Temocillin was the most stable beta-lactam examined, being as stable as cefotetan to most of the beta-lactamases tested. However, cefotetan was hydrolysed at measurable rates by the beta-lactamase of Enterobacter cloacae P99, in contrast to temocillin which was completely stable to this enzyme. Aztreonam was hydrolysed at a slow rate by many of the preparations, being especially labile to the enzyme from Klebsiella oxytoca K1. Cefotaxime was hydrolysed at varying rates by all the preparations.
D000900:Anti-Bacterial Agents / Q000378:metabolism; D001398:Aztreonam; D002439:Cefotaxime / Q000378:metabolism; D015313:Cefotetan; D002513:Cephamycins / Q000378:metabolism; D002876:Chromosomes, Bacterial; D066298:In Vitro Techniques; D010406:Penicillins / Q000378:metabolism*; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002513:Cephamycins; D010406:Penicillins; C031367:temocillin; D015313:Cefotetan; D001618:beta-Lactamases; D001398:Aztreonam; D002439:Cefotaxime