Soluble and immobilized anti-Ig antibodies in the regulation of LPS-induced lymphoblasts.
Anti-immunoglobulin (anti-Ig) causes suppression of secretion of immunoglobulin by LPS-activated pig B lymphoblasts. The cellular level at which anti-Ig influences immunoglobulin secretion has been investigated, using soluble anti-Ig which enters B cells, and anti-Ig immobilized onto acrylamide bead or plastic surfaces which can act only at the B-cell surface. Suppression of secretion only occurred with soluble anti-Ig, indicating that the intracellular processing of antibody after its complexing on the cell surface was necessary for suppression to occur. Immobilized anti-Ig acted as effectively as soluble antibody in activation of resting B cells into mitosis, showing that the activating signal of anti-Ig is received at the cell surface. Electron microscopy has shown that the block to secretion after soluble anti-Ig resulted from the accumulation of smooth membrane-bounded intracellular vesicles which, by immunofluorescence, contained immunoglobulin. The formation of vesicles was intimately associated with the intracellular localization of 125I-labelled anti-Ig which was used to follow cellular processing of anti-Ig.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D002478:Cells, Cultured; D007108:Immune Tolerance; D007075:Immunoglobulin M / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008854:Microscopy, Electron; D008934:Mitogens; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D012995:Solubility; D013552:Swine
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D008934:Mitogens; D011947:Receptors, Antigen, B-Cell