Establishment of Muller cell cultures from adult rat retina.
During a recent study of the effects of photoreceptor degeneration on Muller cells, we observed that unlike glia from normal adult retinas, glia from degenerating rat retinas could be cultured and maintained in vitro for several weeks. Using a variety of cell type specific antisera, we show that at least 70% of cells in these cultures are derived from Muller cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001253:Astrocytes / Q000166:cytology; D002455:Cell Division; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D009457:Neuroglia / Q000032:analysis / Q000145:classification / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000166:cytology*
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein