Denitrification by Alcaligenes eutrophus is plasmid dependent.
Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of converting some nitrate to nitrite, but they did not metabolize nitrite under anaerobic conditions.
D000421:Alcaligenes / Q000235:genetics / Q000378:metabolism*; D000693:Anaerobiosis; D006864:Hydrogenase / Q000235:genetics; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D010957:Plasmids
D009566:Nitrates; D009573:Nitrites; D006864:Hydrogenase