Auto-antibody to kidney tubular cells during retrograde chronic pyelonephritis in rats.
Retrograde pyelonephritis was induced in inbred Fischer rat kidneys with Proteus mirabilis (1 X 10(8) organisms/ml). Sera from pyelonephritic animals sacrificed at 4 and 6 weeks contained cytotoxic antibodies to cultured syngeneic 51Cr-labeled kidney tubular cells (p less than 0.02 and p less than 0.05, respectively) which could be absorbed with plasma membranes. Immune sera from 4- and 6-week pyelonephritic animals also displayed a granular fluorescent pattern along the surface of cultured kidney tubular cells. Quick-frozen syngeneic rat kidney sections stained positively with fluorescent antibody on the intraluminal side of kidney tubular cells. The results suggest that during chronic phases of pyelonephritis, auto-antibodies to kidney tubular cells are induced.
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D007684:Kidney Tubules / Q000276:immunology*; D008297:Male; D011512:Proteus Infections / Q000276:immunology; D011513:Proteus mirabilis; D011704:Pyelonephritis / Q000209:etiology / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344
D001323:Autoantibodies; D007106:Immune Sera