Reduced prostaglandin synthesis by renal and aortic tissues from adult rats fed essential fatty acid-deficient diet after food deprivation.
Studies were conducted to determine the efficacy of a dietary technique for reducing prostaglandin (PG) synthesis in adult rats. Rats weighing 280-318 g were fed either essential fatty acid (EFA)-deficient or EFA-adequate diets for 10-17 days after a period of food deprivation. Synthesis of renal papillary PGE2 and aortic PGI2 from endogenous precursor in vitro were estimated by liquid chromatographic and bioassay/radioimmunoassay techniques, respectively, as indices of the capacity of the technique to induce EFA deficiency. PGE synthesis and PGI2 synthesis by isolated tissues from rats fed the EFA-deficient diet were significantly decreased (ca. 50%) relative to control rats fed an EFA-adequate diet. Body and renal papillary weights were not significantly altered by the EFA-deficient diet.
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis; D005228:Fatty Acids, Essential / Q000172:deficiency*; D005508:Food Deprivation; D007213:Indomethacin / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D004041:Dietary Fats; D005228:Fatty Acids, Essential; D011453:Prostaglandins; D011458:Prostaglandins E; D011464:Epoprostenol; D007213:Indomethacin