Tissue-specific expression of the newly acquired ecotropic Emv-18 provirus in Fv-2 congenic mice.
Expression of endogenous retroviral sequences in Fv-2 congenic mouse strains was examined by Northern blot analysis. Endogenous ecotropic virus transcripts were observed in total spleen RNA of B6.S (Fv-2ss) mice. Endogenous ecotropic transcripts were not detected in spleen RNA of C57BL/6, the Fv-2rr congenic partner of B6.S, nor in spleens of the C57BL/10 (Fv-2rr) and B10.C (Fv-2ss) congenic strains. Mendelian segregation analysis revealed that only backcross mice segregating the newly acquired Fv-2-linked endogenous ecotropic provirus had endogenous ecotropic transcripts in spleen RNA. Examination of different tissues of B6.S mice showed that Emv-18 transcription was highest in spleen and bone marrow, tissues in which Fv-2 has been shown to function. These results support the conclusion that chromosomal location is an important factor controlling Emv-18 expression in B6.S mice. We also report the presence in the spleen of a novel xenotropic virus transcript detectable only in B6.S mice.
D000818:Animals; D002472:Cell Transformation, Viral; D005622:Friend murine leukemia virus / Q000235:genetics; D005786:Gene Expression Regulation; D015683:Gene Products, gag; D051379:Mice; D009857:Oncogenes; D012367:RNA, Viral / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D013154:Spleen / Q000382:microbiology; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D015683:Gene Products, gag; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins