Liquid-chromatographic measurement of purine nucleotides in blood cells.
In this anion-exchange "high-performance" liquid-chromatographic method of analysis for purine nucleotides, the nucleotides are separated with high efficiency and selectivity on a weak anion exchanger (Hypersil APS 2, 3-micron particle size) by elution with a gradient of eluent pH and concentration. Applying this method to analysis for these compounds in human blood cells, we determined them in a patient with adenosine deaminase deficiency who was treated with a bone-marrow transplantation, finding that the transplantation did not entirely correct the patient's abnormalities of purine metabolism.
D000243:Adenosine Deaminase / Q000172:deficiency*; D016026:Bone Marrow Transplantation; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000378:metabolism*; D009700:Nucleoside Deaminases / Q000172:deficiency*; D011685:Purine Nucleotides / Q000097:blood*
D011685:Purine Nucleotides; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase