Antimalarial drug susceptibility testing of Plasmodium falciparum in Thailand using a microdilution radioisotope method.
Antimalarial activity of chloroquine, quinine, mefloquine and halofantrine against 33 strains of P. falciparum isolated from naturally acquired malaria infections in Thailand was determined using a radioisotope microdilution method. A microtitration procedure was used to test isolates of P. falciparum against the 4 drugs simultaneously. The mean ID50 for chloroquine and quinine reflected known resistance to those drugs in Thailand. The mean ID50 for mefloquine and halofantrine showed susceptibility to these drugs. Four isolates of P. falciparum however had markedly decreased susceptibility to mefloquine (ID50 greater than 15 ng/ml); one case of which was confirmed as the first case of RII resistance for mefloquine in Thailand. Several parasite isolates were also observed to have decreased susceptibility to the new drug, halofantrine. These studies strongly recommend that in vitro testing be done in conjunction with field evaluation of new antimalarial drugs.
D000328:Adult; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology* / Q000627:therapeutic use; D002738:Chloroquine / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D006801:Humans; D007928:Lethal Dose 50; D008288:Malaria / Q000188:drug therapy* / Q000469:parasitology; D008297:Male; D015767:Mefloquine; D008826:Microbial Sensitivity Tests; D010616:Phenanthrenes / Q000494:pharmacology / Q000627:therapeutic use; D010963:Plasmodium falciparum / Q000187:drug effects*; D011803:Quinine / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology / Q000627:therapeutic use; D013785:Thailand
D000962:Antimalarials; D010616:Phenanthrenes; D011804:Quinolines; D002738:Chloroquine; D011803:Quinine; C023768:halofantrine; D015767:Mefloquine