Direct current stimulation of bone production in the pony: observations with a diaphyseal osteotomy model.
Electrically induced osteogenesis exhibits a dose response curve and can induce bone formation in the absence of trauma and in nonunions. Electrically induced osteogenesis, using direct electric current (DC) in a third metacarpal diaphyseal osteotomy model, in conjunction with internal fixation and postoperative loading, was investigated. Twelve young adult ponies of mixed sex were divided into 2 treatment groups (A and B) of 3 stimulated and 3 controls each and evaluated, using a specifically designed procedure. Stimulated ponies in both groups were given 20 microA of DC via an implanted bone growth stimulator (BGS); group A ponies were stimulated for 60 days and group B ponies for 120 days. Biocompatibility of the BGS was excellent in all ponies. Radiographically, there was more advanced healing with better vascular development in the ponies stimulated for 60 days compared with controls, but no discernible difference was seen at 120 days. Nuclear medical bone imaging revealed a peak uptake at 4 weeks in stimulated groups and the uptake decreased after 8 weeks. Radiopharmaceutical uptake in stimulated ponies was consistently below control uptake throughout the study. Mean specific gravity (SG) of specimens prepared for testing materials properties for group A and B controls were 2.044 +/- 0.119 and 1.939 +/- 0.065, respectively. The SG for group A and group B stimulated were 1.992 +/- 0.049 and 1.876 +/- 0.031, respectively. The SG of specimens obtained from metacarpi of age-matched control ponies was 2.194 +/- 0.166. The ultimate bending strengths and the flexural moduli of elasticity in 60-day control ponies and stimulated ponies were 57 +/- 8.4, 59 +/- 23.9 MN/m2 and 89 +/- 3.5, 106 +/- 6.5 MN/m2 and in 120-day control ponies and stimulated ponies were 13 +/- 1.0, 13 +/- 3.2 GN/m2 and 13 +/- 2.89, 15 +/- 0.577 GN/m2, respectively. Sixty-day samples and 120-day samples had pending strengths (SU) of 25% and 50% that of unfractured cortex, respectively. These findings indicate that 20 microA of DC stimulation on a diaphyseal osteotomy model may not stimulate sufficient bone production to affect the mechanical properties of the bone. Therefore, based on our research, clinical use of BGS in fresh fracture management cannot be recommended.
D000818:Animals; D004195:Disease Models, Animal; D004599:Electric Stimulation Therapy / Q000379:methods / Q000662:veterinary*; D005260:Female; D005593:Fracture Fixation, Internal / Q000662:veterinary; D050723:Fractures, Bone / Q000601:surgery / Q000628:therapy / Q000662:veterinary*; D006734:Horse Diseases / Q000601:surgery / Q000628:therapy*; D006736:Horses; D008297:Male; D008663:Metacarpus / Q000000981:diagnostic imaging / Q000293:injuries*; D010012:Osteogenesis; D010027:Osteotomy / Q000662:veterinary; D011859:Radiography; D011877:Radionuclide Imaging; D014945:Wound Healing