Allogeneic pregnancy in B-lymphocyte deprived CBA/Ca mice--effects on maternal lymphoid organs and fetal survival.
A number of female CBA/Ca (H2-k) mice were injected with rabbit-anti mouse IgM every 2 days throughout their life cycle in order to prevent their development of immunocompetent B-lymphocytes. The efficiency of the anti IgM-treatment was good in the group of mice used, and all experimental mice almost completely lacked 1) surface Ig-staining cells (immunoperoxidase ABC method), 2) serum IgG (ELISA technique), and 3) Ig-secreting cells (protein A plaque assay). All experimental mice contained a functioning T-cell pool, as recorded in vitro by conA-stimulation and one-way MLC. At an age of 40-60 days these mice were allowed to mate allogeneically with C57/Bl (H-2b) males. No harmful effects of the anti-IgM treatment on the first pregnancy cycle were found, and B-cell deprived mice delivered as many healthy litters as did the controls. It was also observed that the enlargement of the spleen and uterus-draining nodes was of the same magnitude in both experimental and control mice at the end of the first pregnancy.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005333:Fetus / Q000276:immunology*; D007114:Immunization; D007075:Immunoglobulin M / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D008297:Male; D008431:Maternal-Fetal Exchange; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy
D000888:Antibodies, Anti-Idiotypic; D000961:Antilymphocyte Serum; D007075:Immunoglobulin M