Active maturation-promoting factor is present in mature mouse oocytes.
Cytoplasmic extracts of meiotically mature mouse oocytes were injected into immature Xenopus laevis oocytes, which underwent germinal vesicle breakdown within 2 h. Germinal vesicle breakdown was not inhibited by incubation of the Xenopus oocytes in cycloheximide (20 micrograms/ml). Identically prepared extracts of meiotically immature mouse oocytes, arrested at the germinal vesicle stage by dibutyryl cyclic AMP (100 micrograms/ml), did not induce germinal vesicle breakdown in Xenopus oocytes. The results show that maturation-promoting factor activity appears during the course of oocyte maturation in the mouse.
D000818:Animals; D005260:Female; D006133:Growth Substances / Q000502:physiology*; D016200:Maturation-Promoting Factor; D008540:Meiosis; D051379:Mice / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D014982:Xenopus laevis
D006133:Growth Substances; D016200:Maturation-Promoting Factor