title: Production of auto-anti-idiotype antibody during the normal immune response. X. Response to TNP-Ficoll in the chicken.
abstract: The spontaneous production of auto-anti-idiotype (Id) was demonstrated after injection of chickens with trinitrophenylated Ficoll (TNP-F) by: (a) the presence of hapten-augmentable plaque-forming cells (PFC), (b) the ability of serum and of hapten eluates from immune spleen cells to cause hapten-reversible inhibition of anti-TNP plaque formation, and (c) an enzyme-linked immunosorbent assay (ELISA). Tests for anti-Id using the ELISA and hapten-reversible inhibition of PFC correlated very well. As in the mouse, the incidence of hapten-augmentable PFC was reduced by thymectomy and increased by the transfer of TNP-F-immune spleen cells. Hapten-augmentable PFC were also observed during the immune response of chickens to p-azobenzene arsonate-conjugated Brucella abortus.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000378:metabolism; D001323:Autoantibodies / Q000096:biosynthesis* / Q000502:physiology; D001667:Binding, Competitive; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005362:Ficoll / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D009578:Nitrobenzenes / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014301:Trinitrobenzenes / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D006241:Haptens; D007130:Immunoglobulin Idiotypes; D009578:Nitrobenzenes; D011134:Polysaccharides; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll