Expression of the bacteriophage P1 cin recombinase gene from its own and heterologous promoters.
The cin recombinase of bacteriophage P1, a protein that catalyses site-specific DNA inversions, has been identified and its structural gene has been cloned under the control of different promoters. One of the DNA sequences used for the site-specific recombination, cixL, overlaps with the 3' end of the gene, but we show that the presence of this site does not affect cin gene expression from strong promoters. To assay cin activity we have constructed plasmids that carry antibiotic resistance genes within the invertible segment that are transcribed from promoters outside the segment. DNA inversion switches on or off genes for chloramphenicol or kanamycin resistance. These tester plasmids are used to study cin-mediated DNA inversion both in vivo and in vitro.
D001483:Base Sequence; D007446:Chromosome Inversion; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins; D004254:DNA Nucleotidyltransferases; C039930:cin recombinase