Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937.
Phagocytic cells, including normal human blood neutrophils and monocytes, metabolize 25-hydroxyvitamin D3 in vitro to more polar metabolites. Cells of the human monoblastic cell line U937 produced three metabolites when incubated with 25-hydroxyvitamin D3. One of these metabolites, previously designated peak III, has its maximal absorbance at 310 nm. Mass spectral analysis of the trimethylsilylated derivatives of peak III revealed a spectral pattern very similar to that published for the trimethylsilylated derivatives of 19-nor-10-keto-25-hydroxyvitamin D3. The biosynthetic 19-nor-10-keto-25-hydroxyvitamin D3 was active in the induction of differentiation of U937 cells.
D002112:Calcifediol / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D008222:Lymphokines / Q000494:pharmacology; D013058:Mass Spectrometry; D009000:Monocytes / Q000166:cytology*; D010586:Phagocytes / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D008222:Lymphokines; C039030:19-nor-10-keto-25-hydroxyvitamin D3; D002112:Calcifediol