A rapid and sensitive non-competitive avidin-biotin assay for lactoferrin.
We have developed an avidin-biotin assay for the detection of lactoferrin in human seminal plasma. We compared 5 modifications of this assay, and found the non-competitive avidin-biotin assay (NABA) to be the most sensitive. The detection limit of 3-step NABA was 0.2 or 0.1 ng lactoferrin/ml, depending whether avidin-biotin-peroxidase complex (ABC) or avidin-peroxidase was used. The intra-assay and inter-assay coefficients of variation for 3-step NABA were 6.2 and 8.5%, respectively. The lactoferrin levels of human seminal plasma samples measured by 3-step NABA and radioimmunoassay (RIA) showed good correlation (r = 0.96). The total performance time of 3-step NABA is flexible and the method can be modified for rapid (less than 1 h) or overnight assay according to need.
D001360:Avidin; D001710:Biotin; D006801:Humans; D007124:Immunoenzyme Techniques; D007781:Lactoferrin / Q000032:analysis*; D007782:Lactoglobulins / Q000032:analysis*; D008297:Male; D008722:Methods; D010047:Ovalbumin / Q000031:analogs & derivatives; D011863:Radioimmunoassay; D012661:Semen / Q000032:analysis
D007782:Lactoglobulins; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin; D007781:Lactoferrin