The use of microcomputers for the quantitation of light intensity patterns using digitized video signals.
We have developed an inexpensive yet versatile microcomputer-based system for quantitating light intensity levels in autoradiographs. This system employs a standard video camera interfaced to an analog-to-digital convertor. A program has been written for this system which can measure intensities within a defined region of an autoradiograph, permitting an easy and accurate quantitation of spots or bands of irregular shape.
D000465:Algorithms; D000703:Analog-Digital Conversion; D001345:Autoradiography / Q000379:methods; D008838:Microcomputers; D012815:Signal Processing, Computer-Assisted; D012984:Software; D014741:Video Recording