[Change in the strength of DNA-protein binding in T- and B-lymphocytes of the spleen of C3HA mice during chemical hepatocarcinogenesis].
The tightness of DNA-protein binding in the nuclei of mouse spleen T- and B-lymphocytes was assessed, using nucleoprotein celite chromatography, and changes in the number of T- and B-suppressors in the course of o-AAT-induced chemical hepatocarcinogenesis were studied. Attenuation of DNA-protein bonds in T-lymphocytes at the early stages (up to 3 months) was observed, and by the time of hepatoma formation (8 months) about 50% of T-lymphocyte DNA was loosely bound to proteins, which is a typical feature of quiescent cells. In B-lymphocytes attenuation of DNA-protein interaction was only observed by the 8th month of carcinogenesis. By the time of hepatoma formation the number of T-suppressors in mouse spleen increased 2.8-fold, while the number of B-suppressors in lymph nodes remained unchanged.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism*; D008113:Liver Neoplasms / Q000139:chemically induced*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009363:Neoplasm Proteins / Q000378:metabolism*; D011485:Protein Binding; D013154:Spleen / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D009762:o-Aminoazotoluene
D001798:Blood Proteins; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009762:o-Aminoazotoluene
Izmenenie prochnosti sviazi DNK-belok v T- i B-limfotsitakh selezenki mysheĭ linii C3HA pri khimicheskom gepatokantserogeneze.