Topographical studies of lymphocyte localization using an intracellular fluorochrome.
A procedure for analysing the topographical localization in tissue sections or whole-organ mounts of lymphocytes labelled with an intracellular DNA-binding fluorochrome, Hoechst dye No. 33342, is described. The localization of intravenously injected lymphocytes in spleen, popliteal lymph nodes, and Peyer's patches was followed up to 7 days. In the case of spleen, both B and T lymphocytes initially localised in the marginal zone. Subsequently, B cells appeared to exit via the red pulp, while T cells aggregated around vessels in the white pulp. In Peyer's patches, B and T lymphocytes localized to different lymphoid areas. The advantages and potential applications of this technique are discussed.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D001562:Benzimidazoles / Q000378:metabolism; D002465:Cell Movement; D005260:Female; D007267:Injections; D007425:Intracellular Membranes / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010581:Peyer's Patches / Q000166:cytology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology
D001562:Benzimidazoles; C017807:bisbenzimide ethoxide trihydrochloride