Establishment of growth factor-dependent MOPC 104E cell line in vitro.
The MOPC 104E cell line has been adapted to grow in vitro using a combination of feeder layer and growth factor(s). The growth of this myeloma cell line is dependent on the presence of growth factor(s). Growth-promoting activity generated from T-cell-mitogen-stimulated, Corynebacterium parvum-stimulated spleen cell culture supernatant, and peritoneal adherent cell culture supernatants gives dose-dependent proliferation. Generation of growth factors in the serum-free bovine serum albumin-substituted media and a rapid assay system based on [3H]thymidine uptake for the quantitation of growth promoting activity are described.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006128:Growth; D006133:Growth Substances / Q000502:physiology; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D015846:Monokines; D009101:Multiple Myeloma / Q000473:pathology*; D009374:Neoplasms, Experimental / Q000473:pathology*; D010529:Peritoneal Cavity / Q000166:cytology; D011506:Proteins / Q000494:pharmacology*
D003470:Culture Media; D006133:Growth Substances; D008222:Lymphokines; D015846:Monokines; D011506:Proteins; D015847:Interleukin-4