DNA homologies between the genomes of Choristoneura fumiferana and Autographa californica nuclear polyhedrosis viruses.
The DNA sequence homology between the genomes of Choristoneura fumiferana and Autographa californica multicapsid nuclear polyhedrosis viruses (CfMNPV and AcMNPV) were compared by hybridization of nick-translated [32P]CfMNP[V DNA to restricted AcMNPV genome. In the presence of 5 x SSC and 50% formamide the CfMNPV DNA exhibited extensive homology to the AcMNPV genome. When the stringency conditions of hybridization were lowered, we observed hybridization to almost all the EcoRI fragments of AcMNPV. We then utilized the cloned EcoRI fragments from both genomes to obtain more detailed information, and to localize the hybridizing fragments on the EcoRI physical map of AcMNPV. It was clear that some CfMNPV clones hybridized to more than one fragment of the AcMNPV genome indicating that there has been some DNA sequence rearrangement in the AcMNPV genome.
D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D007304:Insect Viruses / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004279:DNA, Viral