Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor.
DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis*; D004247:DNA / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis / Q000235:genetics*; D051381:Rats
D000596:Amino Acids; D011498:Protein Precursors; D004247:DNA; D003346:Corticotropin-Releasing Hormone