Partial purification and characterisation of a 31 kDalton putative enkephalin precursor from guinea pig striatum.
The presence of several putative precursors for Met-enkephalin (ME) and Leu-enkephalin (LE) has been demonstrated in guinea pig striatal extracts. The major high molecular weight (Mr) species (31000) contained 9% of the total ME-immunoreactivity (IR) after digest. This species has been partially purified and characterized using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and nitrocellulose blotting techniques as well as highly specific radioimmunoassay (RIA) for ME, LE and ME-Arg6-Phe7.
D000818:Animals; D043424:Carboxypeptidase B; D002268:Carboxypeptidases; D002384:Catalysis; D003342:Corpus Striatum / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D004745:Enkephalins / Q000302:isolation & purification*; D006168:Guinea Pigs; D008297:Male; D008666:Metalloendopeptidases; D011498:Protein Precursors / Q000302:isolation & purification*; D014357:Trypsin
D004745:Enkephalins; D011498:Protein Precursors; D002268:Carboxypeptidases; D010450:Endopeptidases; D043424:Carboxypeptidase B; D014357:Trypsin; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus