Physicochemical and immunochemical proofs of short-term insulin regulation of hepatic M2 pyruvate kinase.
In isolated rat hepatocytes, the short-term regulation of the M2 pyruvate kinase isozyme by insulin is demonstrated. This hormone dependence, which is a function of dose and incubation time, is checked by the activity of the pyruvate kinase, using enzyme assay, cellulose acetate electrophoresis and electroimmunodiffusion methods.
D000818:Animals; D004588:Electrophoresis, Cellulose Acetate; D007122:Immunoelectrophoresis; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D011770:Pyruvate Kinase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D007328:Insulin; D007527:Isoenzymes; D011770:Pyruvate Kinase