Application of flow cytometry to detection and characterization of Legionella spp.
Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps related to infectivity, in Legionella populations.
D000389:Air Conditioning; D000907:Antibodies, Bacterial / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D007875:Legionella / Q000276:immunology / Q000302:isolation & purification*; D014871:Water Microbiology
D000907:Antibodies, Bacterial