title: Hormonal induction of specific protein synthesis in murine Leydig tumor cells.
abstract: The M5480A murine Leydig cell tumor was used to investigate the effects of three hormones, which produce distinct biochemical actions, on cytoplasmic protein synthesis. Human choriogonadotropin treatment of tumor-bearing mice induced the synthesis of six proteins with relative molecular weights (Mr) of 135K, 82K, 60K, 19K, 18.2K and 17.3K (K = kilodaltons). Diethylstilbestrol induced one protein peak in common with the gonadotropin Mr = 135K) and three additional proteins with Mr's of 120K, 50K and 36K. Epidermal growth factor induced one major protein with Mr = 33K, which is similar to that of a protein induced in murine epidermal cells by tumor promoters. These studies demonstrate the induction of specific gene products in a hormone-responsive tumor.
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007984:Leydig Cell Tumor / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D014176:Protein Biosynthesis; D013736:Testicular Neoplasms / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D004815:Epidermal Growth Factor; D004054:Diethylstilbestrol