Endocytosis in yeast: several of the yeast secretory mutants are defective in endocytosis.
Yeast cells have been shown to internalize lucifer yellow CH by endocytosis. Internalization of the fluorescent dye is time-, temperature-, and energy-dependent, it is not saturable, and the dye is accumulated in the vacuole. Some of the yeast secretory mutants that accumulate endoplasmic reticulum or Golgi bodies are defective for endocytosis at restrictive temperature, while others are not. All of the mutants that accumulate secretory vesicles are defective for endocytosis. These results suggest that efficient transport of proteins from the endoplasmic reticulum to the Golgi apparatus and from the Golgi to secretory vesicles is not necessary for endocytosis. In contrast, endocytosis may be obligatorily coupled with the latest steps of secretion.
D003594:Cytoplasmic Granules / Q000378:metabolism; D004705:Endocytosis; D004734:Energy Metabolism; D005456:Fluorescent Dyes; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D013696:Temperature
D005456:Fluorescent Dyes; D005656:Fungal Proteins; D007546:Isoquinolines; C017475:lucifer yellow