An improved assay for the detection of interleukin 1.
A 24 h, highly sensitive assay for detection of interleukin 1 (IL-1) is described. A thioguanine-resistant mutant of the murine lymphoma cell line LBRM 33 was selected (LBRM TG6). When this cell line was incubated with low concentrations of PHA and IL-1 it produced interleukin 2 (IL-2). IL-2-dependent HT2 cells were co-cultured with the LBRM cells to measure the released IL-2. Prior to addition of tritiated thymidine to the co-culture, hypoxanthine and azaserine were added to metabolically block DNA synthesis by LBRM TG6 cells. This resulted in a sensitive, short term assay requiring minimal technical manipulations and characterized by a high signal to noise ratio.
D000818:Animals; D001377:Azaserine; D002460:Cell Line; D003470:Culture Media; D019271:Hypoxanthine; D007042:Hypoxanthines; D007375:Interleukin-1 / Q000032:analysis*; D008213:Lymphocyte Activation; D008297:Male; D008722:Methods; D051379:Mice; D008809:Mice, Inbred C3H; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology
D003470:Culture Media; D007042:Hypoxanthines; D007375:Interleukin-1; D010835:Phytohemagglutinins; D019271:Hypoxanthine; D001377:Azaserine; D013936:Thymidine