Jacalin: an IgA-binding lectin.
We previously reported that seeds of Artocarpus integrifolia (jackfruit) contain a lectin, which we call jacalin, that is both a potent T cell mitogen and an apparently T cell-independent activator of human B cells for the secretion of immunoglobulins. During the above experiments we noted a massive precipitation in cell cultures stimulated with greater than or equal to 100 micrograms of lectin. In this paper, we show that the precipitate is formed after the interaction of jacalin and the serum protein added to the culture medium. More importantly, we demonstrate that IgA is probably the major serum constituent precipitated by the lectin and that no IgG or IgM can be detected in the precipitates. In secretions such as colostrum, IgA is the only protein precipitated by jacalin. On the basis of this specificity we describe a simple and reliable affinity chromatography procedure for the purification of both human serum and colostrum IgA. Jacalin is a D-Gal binding lectin and should be a useful tool for studying of serum and secretory IgA.
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D003126:Colostrum / Q000276:immunology; D004347:Drug Interactions; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D008222:Lymphokines / Q000302:isolation & purification / Q000494:pharmacology*; D037121:Plant Lectins; D011233:Precipitin Tests; D029584:Prostatic Secretory Proteins; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*
D001798:Blood Proteins; D007070:Immunoglobulin A; D037102:Lectins; D008222:Lymphokines; D037121:Plant Lectins; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; C043696:jacalin