B-cell responses to male-specific antigen(s) in mice.
It has been found that B-cell responses to male-specific antigen(s) can be clearly demonstrated by reversed plaque assays. Female mice injected with syngeneic male spleen cells showed significant increases (greater than 100 X in some strains) in the number of immunoglobulin-secreting cells in lymph nodes draining the injection site. There was a variation in B-cell responsiveness between strains and this correlated only partially with previously reported T-cell responsiveness to the H-Y antigen. C57BL (H-2b) mice were among the most responsive, while CBA (H-2k), (CBA X C57BL)F1, and BALB/c (H-2d) were all much less responsive. These results apparently open up a new approach to the investigation of B-cell responses to male-specific antigen(s).
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006182:H-Y Antigen / Q000276:immunology*; D006579:Heterozygote; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007518:Isoantibodies / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008912:Minor Histocompatibility Loci; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D006182:H-Y Antigen; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies