Human mononuclear cells exposed to staphylococci rapidly produce an inhibitor of neutrophil chemotaxis.
Serum-free cultures of human peripheral blood mononuclear cells from normal volunteers produce an inhibitor of neutrophil chemotaxis when exposed to heat-killed staphylococci. Human neutrophils were exposed to 100-fold dilutions of supernatants from 6-hr cultures, washed repeatedly, and assayed for chemotactic responsiveness with a radiolabel assay. Dilutions of supernatants from cell cultures exposed to staphylococci resulted in a mean chemotaxis of 856 +/- 83 cpm (n = 21), while that for medium-treated neutrophils was 1,354 +/- 100 cpm (n = 21, P less than .001), and supernatants from cultures without staphylococci produced chemotaxis of 1,107 +/- 132 cpm (n = 14, P greater than .05 vs. medium-treated). Fifty-three experiments on tissue from 26 donors showed that after 6 hr the mean inhibition (+/- SE) of chemotaxis at 1:100 dilution was 26.4% +/- 3.3% (P less than .001 vs. medium-treated). It was found that the peptidoglycan fraction of the staphylococci was sufficient to induce production of inhibitor by the cooperative action of T cells and monocytes. The inhibitor is a protein with a molecular mass of 30-45 kilodaltons. It is not toxic to the neutrophils and does not affect secretion, adhesion, phagocytosis, or the ability to kill Staphylococcus aureus. This potent inhibitor of neutrophil chemotaxis may play a role in the modulation of neutrophil function during bacterial infections.
D002465:Cell Movement; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006801:Humans; D008222:Lymphokines / Q000096:biosynthesis* / Q000494:pharmacology; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism*; D009504:Neutrophils / Q000502:physiology*; D010457:Peptidoglycan / Q000494:pharmacology*; D013296:Streptococcus pneumoniae / Q000502:physiology*; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors
D008222:Lymphokines; D010457:Peptidoglycan; C034397:chemotactic inhibitory factor