Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells.
A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37 degrees C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000330:Aedes / Q000235:genetics*; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase; D004247:DNA / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004330:Drosophila / Q000201:enzymology / Q000235:genetics; D005796:Genes; D005822:Genetic Vectors; D006583:Hexadimethrine Bromide / Q000494:pharmacology; D007700:Kinetics; D011401:Promoter Regions, Genetic / Q000187:drug effects; D014162:Transfection
D006583:Hexadimethrine Bromide; D004247:DNA; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D004121:Dimethyl Sulfoxide