Is G2-arrest an active cellular response to irradiation?
Protein synthesis is normally required for G2-cell progression and for recovery from radiation-induced G2-arrest. In the presence of 5 mM caffeine this requirement is alleviated, indicating that the mechanism responsible for G2 cell progression actually remains intact in irradiated or protein synthesis inhibitor-treated cells. It is suggested that both radiation and cycloheximide-induced G2-arrest are not, therefore, passive consequences of cellular defects, but are rather, active cellular responses to the state of cellular integrity, implying the existence of G2 cell progression controls.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000528:radiation effects; D005260:Female; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects*; D010053:Ovary; D014176:Protein Biosynthesis
D002110:Caffeine; D004247:DNA; D003513:Cycloheximide