Immunolocalization of gamma-carboxyglutamic acid-containing proteins in developing molar tooth germs of the rat.
The localization of dentin gamma-carboxyglutamic acid containing proteins (DGP's) was examined in rat molar tooth germs at various stages of development by immunostaining with the peroxidase-antiperoxidase method. Odontoblasts and their processes stained positive, whereas all other cells were negative. No staining was found in predentin. Dentin stained only after a mild demineralization. The results support the concept that DGP's are deposited by odontoblasts directly into the mineralizing dentin.
D015055:1-Carboxyglutamic Acid / Q000378:metabolism*; D000818:Animals; D003804:Dentin / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008903:Minerals / Q000378:metabolism; D009804:Odontoblasts / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014083:Tooth Germ / Q000254:growth & development / Q000378:metabolism*
D005971:Glutamates; D008903:Minerals; D011506:Proteins; D015055:1-Carboxyglutamic Acid