The antigen receptor on a human T cell line initiates activation by increasing cytoplasmic free calcium.
A monoclonal antibody to the antigen-receptor on the T cell line Jurkat induces substantial increases in [Ca++]i. Ca++ ionophores can substitute for this antibody in activation by increasing [Ca++]i to levels comparable with those seen with the antigen-receptor antibody. Stimulation with either the antigen-receptor antibody or a Ca++ ionophore leads to the appearance of the same phosphoproteins. These results suggest that the antigen-receptor initiates T cell activation by increasing [Ca++]i.
D000911:Antibodies, Monoclonal / Q000502:physiology; D002118:Calcium / Q000096:biosynthesis*; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D004987:Ethers / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D015759:Ionomycin; D008213:Lymphocyte Activation / Q000187:drug effects; D010750:Phosphoproteins / Q000096:biosynthesis; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D004987:Ethers; D007376:Interleukin-2; D010750:Phosphoproteins; D011948:Receptors, Antigen, T-Cell; D015759:Ionomycin; D002118:Calcium