Swelling of the brain in mice caused by pertussis vaccine--its quantitative determination and the responsible factors in the vaccine.
Intracerebral injection of vaccine into the mouse induced swelling of the brain. The swelling reached the maximum in the intensity by day 1 and persisted for several days. A method for quantitative determination of the brain-swelling activity of the vaccine was developed. A positive regression coefficient was found only between the brain-swelling and the lymphocytosis-promoting activities. Such activity was no longer shown with the vaccine heat-treated for 30 min at 80 C, but it was restored upon addition of the lymphocytosis-promoting factor (LPF) that caused no brain swelling by itself. The activity, therefore, was ascribed to cooperation of LPF and a certain heat-stable component other than endotoxin contained by pertussis vaccine.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000473:pathology*; D002552:Cerebral Ventricles / Q000187:drug effects / Q000473:pathology*; D004168:Diphtheria Toxoid / Q000633:toxicity*; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations / Q000633:toxicity; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000633:toxicity*; D013745:Tetanus Toxoid / Q000633:toxicity*
D004168:Diphtheria Toxoid; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004338:Drug Combinations; D010567:Pertussis Vaccine; D013745:Tetanus Toxoid