A direct spectrophotometric assay for D-alanine carboxypeptidases and for the esterase activity of beta-lactamases.
A direct spectrophotometric pH indicator method has been devised to assay the activity of the D-Ala carboxypeptidase/transpeptidase of Streptomyces R61, and which should be of general application to D-Ala carboxypeptidases. The substrate employed is N,N'-diacetyl-L-lysyl-D-alanyl-D-lactate. The method allows the determination of steady-state kinetic parameters, and can also be used for the assay of the esterase activity of beta-lactamases against specific depsipeptides.
D002268:Carboxypeptidases / Q000378:metabolism*; D002473:Cell Wall / Q000201:enzymology; D004950:Esterases / Q000378:metabolism*; D006868:Hydrolysis; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000378:metabolism*
D004950:Esterases; D002268:Carboxypeptidases; D002267:Muramoylpentapeptide Carboxypeptidase; D001618:beta-Lactamases