Immunotoxins containing ricin A or B chains with modified carbohydrate residues act synergistically in killing neoplastic B cells in vitro.
Partially deglycosylated (dg) ricin A and B chains were coupled to rabbit anti-human Ig (IT-dg-A) and goat anti-rabbit Ig (IT-dg-B), respectively. The deglycosylation was accomplished by treating intact ricin (before separation into its constituent chains) with a mixture of sodium metaperiodate and sodium cyanoborohydride. The two immunotoxins (ITs) acted synergistically in vitro to kill Daudi cells, albeit to a four-to-eight-fold lesser degree than ITs prepared with native A and B chains.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002051:Burkitt Lymphoma / Q000276:immunology / Q000628:therapy*; D002241:Carbohydrates / Q000032:analysis*; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D004357:Drug Synergism; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D037121:Plant Lectins; D054790:Ribosome Inactivating Proteins, Type 2; D012276:Ricin / Q000031:analogs & derivatives* / Q000494:pharmacology
D002241:Carbohydrates; D037102:Lectins; D037121:Plant Lectins; D054790:Ribosome Inactivating Proteins, Type 2; C040213:modeccin A-chain-ricin B-chain hybrid toxin; D012276:Ricin