Differential expression of class II MHC antigens on cells derived from human tissue.
Human lymphomedullary tissue cells, separated on the basis of density, Fc and E receptors, show characteristic and consistent differences in their expression of class II MHC antigens. A low density, FcR-ve cell type displays high expression of HLA-DR and acts as the most efficient accessory cell in the induction of a Con A driven proliferative response. Exposure of cells to Con A inhibits subsequent binding of anti-HLA-DR antibodies. Addition of -methyl-D-mannoside reverses both the proliferative response to Con A and returns binding of anti-class II MHC antibodies to normal levels. This experimental system can be used to study: the nature of the association between cell bound molecules and class II MHC products, both spatially and functionally; and the relative functional importance of these products in antigen presentation.
D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology*; D002469:Cell Separation; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology*; D014066:Palatine Tonsil / Q000276:immunology; D011961:Receptors, Fc / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D011961:Receptors, Fc; D011971:Receptors, Immunologic