Human alpha 1-antitrypsin expression in Xenopus oocytes. Secretion of the normal (PiM) and abnormal (PiZ) forms.
Injection of equivalent amounts of normal (PiMM) or abnormal (PiZZ) alpha 1-antitrypsin mRNA into Xenopus oocytes resulted in secretion of both the normal and abnormal alpha 1-antitrypsin. A much lower proportion of the abnormal protein was secreted, and the Z alpha 1-antitrypsin that was not secreted accumulated within the cell in a high-mannose form. The time taken for secretion of the normal and abnormal proteins was identical. Both the secreted and intracellular alpha 1-antitrypsin synthesized by oocytes were functionally active.
D000818:Animals; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D009865:Oocytes / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D010641:Phenotype; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000502:physiology; D013347:Subcellular Fractions / Q000378:metabolism; D014981:Xenopus; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase