Comparison of the major histocompatibility antigen on Marek's disease-derived cell lines detected by membrane fluorescent antibody, cytotoxicity, and lymphoagglutination tests.
The reactivity of 13 antisera specific for the major histocompatibility antigen (MHA) of the B complex against cells in 6 cell lines (MDCC-JP1, JP2, HP1, HP2, RP1, and MSB1), derived from Marek's disease (MD) lymphoma, was compared by using membrane fluorescent antibody, cytotoxicity, and lymphoagglutination tests. The membrane fluorescent antibody test was found to be the most sensitive method for detecting the MHA of the B complex on the MD cell lines. After absorption of these antisera with the homologous erythrocytes, the reactivities of the absorbed sera to the homologous erythrocytes and the MD line cells were reduced or lost altogether. However, after absorbing the antisera reactive for MD cell lines with the respective cell line, the reactivities of the absorbed sera to the line cells were not detected at all when examined by the above tests. However, the reactivities of these absorbed sera to the homologous erythrocytes could still be observed when examined by a hemagglutination test. These results indicate that the antisera contain at least two kinds of antibody (against Class I and Class IV antigens), and the three different tests used here are detecting the Class I antigens of MHA on the MD line cells.
D000372:Agglutination Tests; D000818:Animals; D002460:Cell Line; D002645:Chickens; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000032:analysis*; D008223:Lymphoma; D008285:Major Histocompatibility Complex; D008380:Marek Disease
D006649:Histocompatibility Antigens