[Additive nature of the stimulating effects of alloxan diabetes and fasting on the specific binding of insulin with its receptors in the plasma membranes of adipose and hepatic cells in the rat].
Properties of insulin receptors were studied in rat fatty tissue and in plasmatic membranes of liver cells under conditions of alloxan diabetes (within 3 days) and in fasting (within 2 days) as well as under conditions of simultaneous effect of these factors. In the diabetes amount of receptors in plasmatic membranes of both these tissues, affinity of fatty cell membranes as well as amount of the receptors and their specific affinity in hepatic cell membranes were increased. Specific binding of insulin was distinctly increased under conditions of simultaneous influence of diabetes and fasting due to elevation in content of the receptors in liver cell membranes and in both their content and affinity in fatty cell membranes. The data obtained suggest that alloxan diabetes and fasting caused additive effect on expression of insulin receptors in fatty and hepatic cell plasmatic membranes as well as that the content of receptors on the cell surface was regulated by means of several mechanisms.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005215:Fasting; D007328:Insulin / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000378:metabolism*
D007328:Insulin; D011972:Receptor, Insulin
Additivnost' stimuliruiushchikh éffektov alloksanovogo diabeta i golodaniia na spetsificheskoe sviazyvanie insulina s ego retseptorami v plazmaticheskikh membranakh zhirovykh i pechenochnykh kletok krysy.