title: [Primary structure of fragment 7 of the influenza virus A/USSR/90/77(H1N1) RNA].
abstract: The double-stranded DNA copy of the matrix protein (M) gene of the influenza virus A/USSR/90/77 (H1N1) has been inserted in PstI site of plasmid pBR322 and cloned in E. coli. The full-length DNA copy of the M gene has been sequenced using Maxam-Gilbert method. Analysis of nucleotide sequence of segment 7 RNA of influenza virus A/USSR/90/77 and nucleotide substitutions, as compared with known primary structures of segment 7 RNA for other strains, is presented. A hypothetical model of secondary structure of segment 7 RNA of influenza virus and repeating sequences at nucleotide and amino acid levels, revealed in the central region of M1 protein, are discussed.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000032:analysis* / Q000235:genetics; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
vernacular_title: Pervichnaia struktura segmenta 7 RNK virusa grippa A/USSR/90/77(H1N1).