Hormonal regulation of fructose 2,6-bisphosphate levels in epididymal adipose tissue of rat.
The participation of fructose 2,6-bisphosphate on glycolysis stimulated by insulin and adrenaline in incubated white adipose tissue of rat was investigated. Adrenaline addition to incubated fat-pads strongly decreased the intracellular levels of fructose 2,6-bisphosphate. When the tissue was preincubated with glucose, the presence of insulin in the incubation medium increased fructose 2,6-bisphosphate levels 2-fold. These variations were related to changes in the substrates, ATP and fructose 6-phosphate. It therefore appears that fructose 2,6-bisphosphate may be involved in the control of insulin-induced glycolysis, but it does not seem to play a role in the stimulation of glucolysis by adrenaline.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology*; D005635:Fructosediphosphates / Q000096:biosynthesis*; D005636:Fructosephosphates / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006019:Glycolysis; D006598:Hexosediphosphates / Q000096:biosynthesis*; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D005635:Fructosediphosphates; D005636:Fructosephosphates; D006598:Hexosediphosphates; D007328:Insulin; C027618:fructose-6-phosphate; C027652:fructose 2,6-diphosphate; D000255:Adenosine Triphosphate; D005947:Glucose; D004837:Epinephrine