A direct technique for the preparation of chromosomes from early equine embryos.
A technique is described for the preparation of banded chromosomes from early equine embryos cultured for less than 10 h in a medium containing bromodeoxyuridine. In addition to standard Giemsa staining and C-banding, chromosomes thus prepared can also be R-banded by either the RBA or the RB-FPG methods. This technique is rapid, repeatable, and limits cell loss, making it ideal for the preparation of early embryos.
D000818:Animals; D002417:Cattle; D002871:Chromosome Banding / Q000379:methods*; D005260:Female; D006736:Horses / Q000235:genetics*; D008940:Mitotic Index; D011247:Pregnancy