A modified immunoperoxidase method for the detection of ABH tissue isoantigens in patients with bladder carcinoma.
Detectable ABH blood group antigens (BGAg) on tumor cells from transitional carcinoma of the urinary bladder, used for diagnosis and prognosis may be evidenced with the existing immunoperoxidase methods. In this study, a modification of the immunoperoxidase method was shown to enable the detection of BGAg in deparaffinized tissue section of normal and malignant human urothelium. To detect antigens A and B a peroxidase-conjugated rabbit anti-human IgM was used and antigen H was visualized by biotinylated lectin.
D000017:ABO Blood-Group System / Q000276:immunology*; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000032:analysis*; D001743:Urinary Bladder / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D000017:ABO Blood-Group System; D007519:Isoantigens