In vivo effects of epidermal and fibroblast growth factors on DNA replication in mouse skin.
A method was developed for measuring in vivo DNA synthesis after exposure to epidermal growth factor (EGF) or fibroblast growth factor (FGF) in a local area of mouse skin using ring-shaped forceps in combination with autoradiography. The technique should be useful for analysing the effects of growth factors on individual cells of the skin in vivo. EGF induced semisynchronized DNA synthesis in basal cells of the epidermis dose-dependently, but FGF did not. Time course study showed that EGF-induced DNA synthesis in basal cells increased with time for 24 h, and then decreased rapidly. EGF-induced DNA synthesis in basal cells was proportional to the time exposed to EGF (0-60 min). FGF and EGF both had little effect on dermal fibroblastic cells. The discrepancy between in vivo observations and those with cultured mammalian cells is discussed.
D000818:Animals; D001345:Autoradiography; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D000078404:Epidermal Cells; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor