[Semen evaluation: technic of study].
Sampling precautions are particularly important in the study of sperm: the length of sexual abstinence, complete collection of the ejaculate, sampling by masturbation in the laboratory. The examination of sperm consists of different steps: measurement of the ejaculate volume, evaluation of the mobility, speed, and propulsion direction of the spermatozoa, study of spermatozoon concentration, estimation of the percentage of live spermatozoa, morphological examination of the spermatozoa, including an enumeration of the abnormal forms, evaluation of spermatozoon survival in vitro for 24 h by monitoring the percentage of mobility every 2 h. This examination can be complemented by biochemical and enzymatic assays of: fructose, zinc, carnitine, citric acid, acid phosphatase, alpha glucosidase, which serve as tracers of associated secretions.
D002470:Cell Survival; D003584:Cytological Techniques; D006801:Humans; D007962:Leukocytes / Q000473:pathology; D008297:Male; D008418:Masturbation; D012661:Semen / Q000166:cytology* / Q000502:physiology; D012746:Sexual Abstinence; D013076:Sperm Count / Q000379:methods; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000166:cytology*; D014783:Viscosity
Spermogramme: technique de réalisation.