Purification and characterization of a low molecular mass cysteine proteinase inhibitor from human amniotic fluid.
We have purified the human low molecular mass cysteine proteinase inhibitor in good yield from amniotic fluid, using ultrafiltration through 100-kDa and 1-kDa cut-off filters, chromatography on Ultrogel AcA 54, and affinity chromatography on alkylated papain-agarose. Approximately 1-4 mg/l of this inhibitor are present in amniotic fluid. The purified inhibitor had an apparent molecular mass of 10.5-12 kDa, as judged by its electrophoretic behavior. Amino acid analysis showed it to be rich in acidic and aliphatic residues and in cysteine. No carbohydrate side-chains could be demonstrated. The purified inhibitor inhibited papain, ficin, cathepsins B, C, and H, the cathepsin B-like enzyme from B16 melanoma cells, and a bovine chromaffin granule enkephalin-converting activity. No inhibition of Ca2-dependent neutral cysteine proteinase, serine- or metallo-proteinases was seen. Analysis of the purified inhibitor by isoelectric focusing revealed 7 major bands with pI values of 7.95, 7.0, 6.7, 6.55, 6.25, 5.5, and 5.2, all of which inhibited papain.
D000596:Amino Acids / Q000032:analysis; D000653:Amniotic Fluid / Q000032:analysis*; D000818:Animals; D002401:Cathepsin B; D002403:Cathepsins / Q000037:antagonists & inhibitors; D002417:Cattle; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A; D003546:Cysteine Endopeptidases; D015853:Cysteine Proteinase Inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010206:Papain / Q000032:analysis; D011247:Pregnancy; D011480:Protease Inhibitors; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification
D000596:Amino Acids; D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors; D011506:Proteins; D003208:Concanavalin A; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D010206:Papain