Immunohistochemical localization of 6-keto-PGF1-alpha in canine coronary vasculature.
The localization of the prostacyclin metabolite, 6-keto-PGF1-alpha, in canine coronary vasculature was accomplished using immunohistochemical techniques (avidin-biotin method of immunoperoxidase staining). Six-keto-PGF1-alpha was localized to the intimal endothelial cell layer of epicardial and intramyocardial arteries and veins. No specific staining was seen in the the media or adventitia of canine coronary vasculature, or in capillaries, or myocardial fibers. To our knowledge these studies represent the first immunohistochemical demonstration of the endothelial cell localization of the prostacyclin metabolite, 6-keto-PGF1-alpha. The described technique allows the cellular localization of prostaglandin metabolites in histologic sections.
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis*; D000818:Animals; D003331:Coronary Vessels / Q000166:cytology*; D004285:Dogs; D004727:Endothelium / Q000166:cytology; D007124:Immunoenzyme Techniques; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D009206:Myocardium / Q000166:cytology*
D015121:6-Ketoprostaglandin F1 alpha