[The effect of 2-deoxy-2-fluoroglucose on the functional maturation of monolayer-cultured B cells of the neonatal rat].
Monolayer islet cells prepared from neonatal rat pancreases were cultured in media with 5.5 mM glucose alone or supplemented with 1 mM 2-deoxy-2-fluoroglucose for a total of 7 days. The recovery of insulin in the cells and media, and the insulin secretory responses to four secretagogues were determined on several occasions. Under culture conditions with a basal medium containing 5.5 mM glucose, the responses to insulin secretagogues tested were abolished after 7 days of culture. In contrast, the addition of 2-deoxy-2-fluoroglucose maintained the insulin secretory responses to glucose (2.8 approximately 33.4 mM), leucine (2.5 approximately 10 mM) and 2-ketoisocaproate (2.5 approximately 10 mM) at levels several times higher than at day 0. Moreover, in these monolayer islet cells the rates of 0.62 mM [U-14C]glutamine oxidation were significantly increased by leucine or 2-ketoisocaproate at 10 mM. From these results, it is suggested that a series of metabolic adaptations in response to change in nutritional status (possibly attributable to the action of 2-deoxy-2-fluoroglucose as an inhibitor of glycolysis) may facilitate the metabolism of amino acids in B cells. Such an activation may be associated with the survival and maturation of neonatal B cells in vitro.
D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004247:DNA / Q000378:metabolism; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019788:Fluorodeoxyglucose F18; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media; D003837:Deoxy Sugars; D007328:Insulin; D019788:Fluorodeoxyglucose F18; D004247:DNA; D003847:Deoxyglucose