Ultrastructural localization of a peroxisomal protein in rat liver using the specific antibody against the non-specific lipid transfer protein (sterol carrier protein 2).
An antibody against the non-specific lipid transfer protein from rat liver was purified by immunoabsorbent affinity chromatography. This antibody in conjunction with protein A-colloidal gold was used to localize the transfer protein in rat liver by electron microscopy. Labeling by this immunocytochemical technique was found to be mainly restricted to the peroxisomes; low labeling was observed in the cytoplasm. Subsequent analysis of isolated peroxisomes by immunoblotting indicated that the non-specific lipid transfer protein (mol. wt. 14800) was absent from this organelle and that a protein of molecular weight 58000 was responsible for the immunological response. Immunoblotting of the membrane-free cytosol showed the presence of both proteins. It remains to be established to what extent the non-specific lipid transfer protein in the cytosol and the high-molecular weight protein in the peroxisomes are related.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D002846:Chromatography, Affinity; D003600:Cytosol / Q000032:analysis; D007163:Immunosorbent Techniques; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008722:Methods; D008830:Microbodies / Q000032:analysis*; D008854:Microscopy, Electron; D008970:Molecular Weight; D010940:Plant Proteins; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D002352:Carrier Proteins; D010940:Plant Proteins; C072087:sterol carrier proteins