Assessment of neutrophil function in the elderly using antibody coated polyacrylamide gel (immunobeads) and nitroblue tetrazolium (NBT) reduction as a combined test.
Neutrophil function was assessed in 35 elderly individuals (age greater than 75) and 20 normal young individuals (age 20-45) by combining ingestion of antibody coated polyacrylamide gel and nitroblue tetrazolium reduction in a single test. This test evaluates phagocytosis and metabolic integrity simultaneously and appears to be a sensitive and reliable test of neutrophil function. No significant difference was found (by using this test) between neutrophils from healthy elderly people and the neutrophils from young controls, or between the sexes in either age group.
D000180:Acrylic Resins; D000328:Adult; D000368:Aged; D000375:Aging; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008863:Microspheres; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D010587:Phagocytosis; D013778:Tetrazolium Salts
D000180:Acrylic Resins; D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D013778:Tetrazolium Salts; C016680:polyacrylamide gels; D009580:Nitroblue Tetrazolium