Regulation of human B lymphopoiesis: effect of a urinary activity associated with cyclic neutropenia.
Urine from a patient with cyclic neutropenia was found to contain a lymphopoietic activity that acts as a growth factor for human pre-B cells. This biologic activity was detectable during the week preceding, but not during, the period of neutropenia. This corresponded with a periodic excessive accumulation of pre-B cells in the marrow of this patient. Urine preparations were added to cultures of normal human bone marrow that had been depleted of B cells. Pre-B cells were generated in these cultures but not in cultures containing urine preparations from normal donors. Pre-B cells were also generated from bone marrow that had been depleted of 177.17+ cells and the majority of pre-B cells. This is the first report of a hemopoietic activity which affects human pre-B cells. This activity may represent a normal regulatory molecule that is periodically produced in excess in this patient.
D000328:Adult; D000380:Agranulocytosis / Q000276:immunology*; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D001666:Binding Sites, Antibody; D001854:Bone Marrow Cells; D002675:Child, Preschool; D006133:Growth Substances / Q000502:physiology / Q000652:urine*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology; D006801:Humans; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology / Q000652:urine*; D008297:Male; D009503:Neutropenia / Q000276:immunology* / Q000652:urine; D010507:Periodicity
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D008222:Lymphokines; D015847:Interleukin-4