Interferon-induced protein Mx accumulates in nuclei of mouse cells expressing resistance to influenza viruses.
In mouse cells carrying the dominant influenza resistance allele Mx+ (but not in Mx- cells) interferon-alpha/beta (IFN) induces an efficient antiviral state against influenza viruses and, concomitantly, the synthesis of a 75,000-Da protein (protein Mx). Here, indirect immunofluorescence using monoclonal antibodies was used to demonstrate that protein Mx accumulates in the nucleus of IFN-treated Mx+ cells, suggesting a nuclear site of action. Protein Mx is present in the nucleus of untreated influenza virus-resistant macrophages freshly explanted from the peritoneal cavity of Mx+ mice but is lost with time in culture when peritoneal macrophages become permissive for influenza virus.
D000483:Alleles; D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins; D007113:Immunity, Innate; D007370:Interferon Type I / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D064547:Myxovirus Resistance Proteins; D009975:Orthomyxoviridae / Q000276:immunology*; D011506:Proteins / Q000235:genetics*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D011506:Proteins; D019204:GTP-Binding Proteins