A highly restricted antigen for renal cell carcinoma defined by a monoclonal antibody.
Monoclonal antibodies against renal cell carcinoma (RCC) antigens were generated by immunizing Balb/c mice with the human RCC cell line 7860. After cloning many RCC reactive monoclonal antibodies, one antibody (D5D), was of special interest. Specificity testing against 33 human tumor cell lines revealed D5D to have no specific reactivity with 17 specimens of normal adult or fetal kidney. Reactivity with 67 specimens of nonrenal tissue demonstrated no reactivity. The reactivity of D5D with 15 different RCC tissues has been variable, being present in 60% of cases. Preliminary data, however, suggest that the antigen may be reexpressed for some of the remaining 40% during in vitro propagation.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm