title: Effect of adenine, cytidine and guanosine on the expression of the SOS system in Escherichia coli.
abstract: Addition of cytidine or guanosine to UV-irradiated cells of a RecA+ strain of Escherichia coli did not produce any effect on the induction of two SOS functions: inhibition of cell division and expression of the umuC gene. Under the same conditions adenine gave a slight increase in the induction of these two responses. In a RecA441 mutant growing at 42 degrees C, both cytidine and guanosine inhibited these SOS functions, whereas adenine produced a large increase in their expression. Moreover, the ATP concentration of the RecA441 mutant at 42 degrees C showed a decrease which occurred earlier in the cells growing in the presence of cytidine or guanosine than in the absence of either compound. Adenine induced an increase of about three times the initial ATP concentration of this mutant at 42 degrees C which dropped quickly after 10 min. Neither cytidine nor guanosine increased the evolution of cellular ATP in UV-irradiated cells of the RecA+ strain, whereas adenine had only a slight positive effect. However, in UV-irradiated RecA+ cells with and without adenine, ATP levels dropped quickly to the initial value after 20 min. These data suggest that the influence of adenine, cytidine and guanosine on the expression of the RecA441 phenotype at 42 degrees C may be due to alteration of the cellular ATP concentration of this mutant.
mesh_terms: D000225:Adenine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003562:Cytidine / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D005786:Gene Expression Regulation; D006151:Guanosine / Q000494:pharmacology*; D009154:Mutation; D014176:Protein Biosynthesis; D011938:Rec A Recombinases / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D006151:Guanosine; D003562:Cytidine; D000255:Adenosine Triphosphate; D011938:Rec A Recombinases; D000225:Adenine