Regulation of the production of granulocyte-macrophage colony-stimulating factor by macrophage-like tumour cell lines.
Macrophage tumour cell lines (PU5-1.8, P388D1) produced detectable granulocyte macrophage colony-stimulating (CSF-2) activity measured using a factor-dependent cell line FDC-P1. The production of CSF-2 was enhanced by endotoxin and inhibited by serum, and correlated inversely with [3H]TdR incorporation. mRNA isolated from PU5-1.8 or P388D1 cells initiated CSF-2 production when injected into Xenopus laevis oocytes. The specific activity in this assay was unaltered in mRNA isolated from endotoxin-treated cells. The results suggest that endotoxin acts at a post-transcriptional level.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D007941:Leukemia P388 / Q000378:metabolism; D007942:Leukemia, Experimental / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D014982:Xenopus laevis
D003115:Colony-Stimulating Factors; D004731:Endotoxins