title: An antisuppressor mutant of Saccharomyces cerevisiae deficient in isopentenylated tRNA has reduced delta 2-isopentenylpyrophosphate: tRNA-delta 2-isopentenyl transferase activity.
abstract: We have previously reported the isolation and initial characterization of a mutation in Saccharomyces cerevisiae, designated mod5-1, that reduces the capacity of altered tyrosine tRNAs to suppress ochre nonsense mutations. The mutation results in the virtual elimination of the modified tRNA nucleoside, N6-delta 2-(isopentenyl) adenosine, normally found adjacent to the anticodons of certain tRNA species. We demonstrate here that MOD5 codes for delta 2-isopentenylpyrophosphate: tRNA-delta 2-isopentenyl transferase, or a protein that regulates its synthesis.
mesh_terms: D019883:Alkyl and Aryl Transferases; D045782:Hemiterpenes; D009154:Mutation; D009943:Organophosphorus Compounds / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D014166:Transferases / Q000378:metabolism*
chemical_list: C025669:2-isopentenyl tRNA; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; C004809:isopentenyl pyrophosphate; D012343:RNA, Transfer; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C068392:MOD5 protein, S cerevisiae; C023197:tRNA isopentenyltransferase