title: mRNA splicing efficiency in yeast and the contribution of nonconserved sequences.
abstract: A simple kinetic model for mRNA splicing predicts the way in which in vivo steady state precursor RNA levels (P) and messenger RNA levels (M) vary as a function of the rate constant of the splicing reaction (ksp). The model points to M/P as the best measure of ksp. The analysis of a set of intron mutations in a yeast gene supports the general features of the model and shows that the splicing efficiency of transcripts containing the wild-type intron is well in excess of what is necessary to generate normal mRNA levels. The data also suggest that regions of the intron, in addition to the well-conserved consensus sequences, contribute to efficient splicing.
mesh_terms: D001483:Base Sequence; D004274:DNA, Recombinant; D005800:Genes, Fungal; D007700:Kinetics; D008957:Models, Genetic; D009154:Mutation; D009694:Nucleic Acid Precursors / Q000235:genetics / Q000378:metabolism*; D012322:RNA Precursors; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012331:RNA, Fungal; D012333:RNA, Messenger