The development of a solid phase radioimmunoassay for the detection of leishmanial parasites in the sand fly.
The development of a solid phase radioimmunoassay inhibition test using anti-leishmanial monoclonal antibodies is described for the detection of leishmanial promastigote infections in sand flies, as an aid to epidemiology. As few as 6-12 Leishmania major promastigotes could be detected in laboratory-bred Phlebotomus papatasi. The test was sensitive and species-specific.
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D007891:Leishmania / Q000276:immunology; D010691:Phlebotomus / Q000469:parasitology*; D011863:Radioimmunoassay; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan