Effects of dicyclohexane derivatives on androgen metabolism in the rat prostate.
Dicyclohexane derivatives, which inhibit the binding of testosterone and dihydrotestosterone (DHT) to the androgen-binding protein (ABP) of rat epididymis without interfering with their binding to the androgen receptor, show a similar selectivity in their effects on androgen metabolism. Their ability to inhibit the aromatization of testosterone has been reported previously. This paper demonstrates that they are potent inhibitors of 3 alpha(beta)-hydroxysteroid:NAD(P)+ oxidoreductase activity (3-HSD) in the particulate fraction from rat prostate gland; the values of Ki for their inhibition of this enzyme are similar to that of the Km for DHT as substrate. The dicyclohexane derivatives are markedly less effective against the cytosolic NADPH-dependent 3-HSD, and they do not appear to inhibit testosterone 5 alpha-reductase activity. These characteristics are likely to complicate the proposed use of the dicyclohexane derivatives as probes for the role of ABP in vivo. However, they may be of interest in the study of structure-activity relationships in androgen-metabolizing enzymes, particularly in the examination of the different forms of 3-HSD.
D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors; D000727:Androgen-Binding Protein / Q000378:metabolism; D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D042944:Cholestenone 5 alpha-Reductase; D003510:Cyclohexanes / Q000494:pharmacology*; D013196:Dihydrotestosterone / Q000378:metabolism; D008297:Male; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013739:Testosterone / Q000378:metabolism
D000727:Androgen-Binding Protein; D000728:Androgens; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D003510:Cyclohexanes; D013196:Dihydrotestosterone; D009243:NAD; D013739:Testosterone; D009249:NADP; D010088:Oxidoreductases; D015096:3-Hydroxysteroid Dehydrogenases; D042944:Cholestenone 5 alpha-Reductase