Single acetylcholine channel currents in sympathetic neurons.
Single acetylcholine (ACh) channel currents were studied by the gigaohm patch-clamp technique in cultured sympathetic neurons of the bullfrog, Rana catesbeiana. Recordings were made at 22 degrees C on cell-attached and excised membrane patches. When ACh (0.5-1 microM) was present in the pipette, a single class of inward currents was observed with a chord conductance of 30 pS and a reversal potential of -2 mV. The mean channel open time was 11.6 ms at -65 mV and showed little or no voltage-dependence over the range -85 to -45 mV. These channels appear to mediate the fast nicotinic excitatory postsynaptic current.
D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D005728:Ganglia, Sympathetic / Q000502:physiology*; D008564:Membrane Potentials; D009474:Neurons / Q000502:physiology*; D011892:Rana catesbeiana
D000109:Acetylcholine