Amphibian lymphokines: 1. Leucocyte chemotactic factors produced by amphibian spleen cells following antigenic and mitogenic challenge in vitro.
The production of soluble chemotactic factors by Rana temporaria leucocytes following in vitro stimulation with antigen or mitogen was examined. Stimulated spleen cells were found to produce a soluble factor with a molecular weight (MW) of between 16,000-27,000 daltons which was chemotactic for Rana peritoneal cells. Rana peritoneal cells also showed directional movement towards solutions of casein, which is a potent chemo-attractant for mammalian lymphoid cells.
D000818:Animals; D000941:Antigens / Q000276:immunology; D001202:Ascitic Fluid / Q000276:immunology; D002364:Caseins / Q000276:immunology; D002630:Chemotactic Factors / Q000096:biosynthesis*; D003208:Concanavalin A / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D066298:In Vitro Techniques; D008222:Lymphokines / Q000096:biosynthesis*; D008970:Molecular Weight; D011896:Rana temporaria / Q000276:immunology*; D013154:Spleen / Q000276:immunology*
D000941:Antigens; D002364:Caseins; D002630:Chemotactic Factors; D008222:Lymphokines; D003208:Concanavalin A