Extracellular material secreted by human colonic adenocarcinoma cell lines promotes spreading in serum-free medium and induces neurite outgrowth of PC-12 cells.
A completely defined medium has been designed to promote cell proliferation of 2 colonic adenocarcinoma cell lines of epithelial origin (HT 29 and HRT 18). The spreading of both cell types, especially of HT 29 cells, was not possible in a serum-free medium supplemented with growth factors. Spreading was obtained in a defined medium (a 1/1 mixture of DMEM and F12 media supplemented with 5 micrograms/ml transferrin, 5 ng/ml EGF, 10 ng/ml selenite and 15 mM HEPES pH 7.3) with an extracellular matrix-like material (ECM) secreted by the cells themselves. The properties of the ECM have been studied: ECM secreted by the 2 cell lines induced very quick spreading of HT 29 and HRT 18 cells (1 to 2 hr vs. 12 to 24 hr in serum-supplemented medium). ECM induced morphological differentiation of a rat pheochromocytoma cell line (PC 12). PC-12 cells grown under these conditions began to develop neurite extensions as early as 2 hr after seeding.
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000310:Adrenal Gland Neoplasms / Q000473:pathology*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005109:Extracellular Matrix / Q000502:physiology*; D006801:Humans; D010673:Pheochromocytoma / Q000473:pathology*; D051381:Rats
D003470:Culture Media; D004815:Epidermal Growth Factor