Cell-mediated T lymphocyte responses against syngeneic cells modified with amino-reactive hapten (AED-NH2): H-2Dk serves as an element for cell-mediated lympholysis to amino-reactive hapten (AED-NH2)-modified self.
Spleen cells from C3H/He mice immunized to the newly synthesized amino-reactive hapten, 5-sulfo-1-naphthoxy acetic acid N-hydroxysuccinimide (AED-NH2), were stimulated in vitro with AED-NH2 modified syngeneic cells. After 5 days of culture, effector cells were assayed for their cytotoxic activity against AED-NH2-modified target blast cells. In contrast to other amino-reactive haptens reported so far, a strong cytotoxic activity against AED-NH2-modified syngeneic cells was found in H-2b mice as well as in H-2k mice. Furthermore, Dk-restricted anti-AED-NH2 CTL recognition was observed in H-2k mice as shown by cold target inhibition. Previous studies have demonstrated the predominant influence of K over D region self determinants, and of the chemical reactivity of the haptenic reagent in Ir gene control of CTL response to hapten-self. The present report illustrates the importance of the hapten itself in genetic regulation of these CTL responses.
D000818:Animals; D001667:Binding, Competitive; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology*; D064532:Histocompatibility Antigen H-2D; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009280:Naphthaleneacetic Acids / Q000008:administration & dosage / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage / Q000276:immunology
D006183:H-2 Antigens; D006241:Haptens; D064532:Histocompatibility Antigen H-2D; D009280:Naphthaleneacetic Acids; C040132:5-sulfo-1-naphthoxyacetic acid N-hydroxysuccinimide ester; D014302:Trinitrobenzenesulfonic Acid