Tumour inhibitory effects of TCGF/IL-2/-containing preparations.
Supernatants from ConA-stimulated rat spleen cell cultures and from cultures of PMA-stimulated murine lymphoma subline EL-4TF were found to contain TCGF and to inhibit growth of a transplantable, MC-induced sarcoma MC11 in syngeneic mice. Tumour-inhibitory effects of the supernatants were dependent on local and repeated administration. Prior to use of the supernatants obtained from PMA-stimulated EL-4TF cell cultures, the dialysable PMA had to be removed; contamination with PMA was found to abolish the tumour-inhibitory effect of the supernatants and to produce enhancement of tumour growth. A significant tumour-inhibitory effect has also been obtained with partially purified TCGF prepared from culture supernatants of cloned EL-4TF cells by ammonium sulphate precipitation, ion-exchange (FPLC) chromatography, and AcA 44 Ultrogel filtration.
D000818:Animals; D007167:Immunotherapy; D007376:Interleukin-2 / Q000302:isolation & purification / Q000627:therapeutic use*; D051379:Mice; D051381:Rats; D012513:Sarcoma, Experimental / Q000473:pathology / Q000628:therapy*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D007376:Interleukin-2; D013755:Tetradecanoylphorbol Acetate