A new Tla region antigen Qa-11, similar to Qa-2 and associated with B-type beta 2-microglobulin.
A new antigen, Qa-11, is detected as a 40,000 dalton band in the SDS-PAGE of immunoprecipitates of radiolabeled lymphocyte membrane preparations. In C57BL H-2 congenic strains, its presence is controlled by a gene in the Tla region. In strains with genetic background other than C57BL it is not expressed. Tests with recombinant inbred strains and with H-3 congenic strains show that, in addition to the Tla region, a gene linked to or identical with the beta 2-microglobulin-b-allele is required for the expression of Qa-11 as well. The mobility of the Qa-11 antigen in SDS-PAGE and in isoelectrofocusing is the same as that of Qa-2 antigen. The Cleveland peptide maps of Qa-2 and Qa-11 are identical as well. This finding, that the Tla region controlled Qa-11 antigen is structurally very similar to the Qa-2 antigen, contrasts with the fact that Tla region products do not react with anti-Qa-2 sera. This paradox could be explained by a separate Qa-11 region between Qa-2 and Tla. Alternatively, it is possible that the Qa-11 antigen is the result of the action of a modifying gene in the Tla region upon a Qa-2 gene product, or that the structural gene for Qa-11 is located in the Qa-2 region and a Tla region gene controls its expression.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D011233:Precipitin Tests; D001613:beta 2-Microglobulin / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments; C032907:Q surface antigens; D001613:beta 2-Microglobulin