[Topography of cysteine residues in DNA-dependent RNA-polymerase].
Chemical modification of DNA-dependent RNA polymerase with monoiod-[14C]acetic acid and N-[14C]ethylmaleimide has been carried out and position of superficial and functionally important as well as enzymatically non-significant, exposed cysteine residues have been localised in the enzyme. Topography of cysteine residues in the RNA-polymerase alpha-subunit is thoroughly studied. The results are summarized in a model.
D000595:Amino Acid Sequence; D001345:Autoradiography; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine / Q000032:analysis*; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D006868:Hydrolysis; D007461:Iodoacetates; D019807:Iodoacetic Acid
D007461:Iodoacetates; D012321:DNA-Directed RNA Polymerases; D003545:Cysteine; D019807:Iodoacetic Acid
Topografiia ostatkov tsisteina v DNK-zavisimoĭ RNK-polimeraze.