Evaluation of 1,3-pentadiene for mutagenicity by the Salmonella/mammalian microsome assay.
1,3-Pentadiene, a food contaminant produced by some molds when they metabolize sorbic acid, was tested for mutagenicity, using variations of the Salmonella/mammalian microsome assay. The chemical was incorporated into the test system (with and without S9 mix) by 3 methods: (a) the standard plate incorporation assay, (b) a liquid preincubation procedure and (c) exposure of test bacteria in the soft agar overlay to gaseous 1,3-pentadiene. The chemical was extremely toxic to the test bacteria with amounts as low as 2.0 microgram/plate causing cell death. However, none of the nonlethal concentrations tested by any of the methods was mutagenic to Salmonella typhimurium strains TA97, TA98, TA100, TA1535, TA1537 or TA1538.
D000466:Alkadienes / Q000494:pharmacology*; D000818:Animals; D001711:Biotransformation; D005506:Food Contamination; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D009154:Mutation; D010420:Pentanes; D012486:Salmonella typhimurium / Q000187:drug effects; D013045:Species Specificity
D000466:Alkadienes; D009153:Mutagens; D010420:Pentanes; C011970:1,3-pentadiene