title: Kinetic studies of oestradiol 17 beta-hydroxysteroid dehydrogenase in MCF-7 mammary cancer cells.
abstract: The kinetic properties of oestradiol 17 beta-dehydrogenase have been studied in the MCF-7 human breast cancer cell line. The activity of the enzyme was found to be linear with respect to time for at least 2 h and with respect to protein concentrations over the range 40-300 micrograms protein per tube. The enzyme was able to utilize both NAD and NADP as cofactors but at higher concentrations NAD was the more effective. The apparent Km was estimated to be 13.4 microM which is approximately two-fold higher than found for breast tumour tissue.
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology*; D002460:Cell Line; D004960:Estradiol Dehydrogenases / Q000378:metabolism*; D004970:Estrone / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism
chemical_list: D009243:NAD; D004970:Estrone; D009249:NADP; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases