Intracellular human IL-1: a precursor for the secreted monokine.
An intracellular monocyte derived protein possessing interleukin 1 (IL-1) activity has been compared with the secreted from of IL-1. Our results indicated that this intracellular IL-1 had an apparent size greater than that of extracellular IL-1. Data are presented to show that a rabbit heterotypic antiserum prepared against epitopes on the secreted form of human IL-1 blocked the biologic activity of intracellular IL-1 in a dose dependent manner. Intracellular IL-1 had a mass of 30-40 kDa as determined by gel filtration using sephacryl-200 gel.
D003593:Cytoplasm / Q000502:physiology; D005110:Extracellular Space / Q000502:physiology; D006133:Growth Substances / Q000302:isolation & purification; D006801:Humans; D007375:Interleukin-1 / Q000302:isolation & purification / Q000502:physiology*; D015847:Interleukin-4; D008222:Lymphokines / Q000302:isolation & purification; D008264:Macrophages / Q000502:physiology; D008970:Molecular Weight; D009000:Monocytes / Q000502:physiology; D015846:Monokines; D011498:Protein Precursors / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*
D006133:Growth Substances; D007375:Interleukin-1; D008222:Lymphokines; D015846:Monokines; D011498:Protein Precursors; D011506:Proteins; D015847:Interleukin-4