A large scale method of separating multiple lymphokines secreted by the murine EL-4 thymoma.
The in vivo administration of lymphokines is a new approach to immunotherapy with possible applications to the treatment of tumors and infectious processes. These lymphokines are produced in very small quantities and purification of these molecules has proven difficult. A rapid two step method was used to isolate different lymphokines from large quantities of conditioned media produced by the murine EL-4 cell line. Interleukin-2 (IL-2), colony stimulating factor (CSF) and macrophage activating factor (MAF) are lymphokines with distinct biologic activities and are secreted by the EL-4 cell line. Their isolation involved initial batch purification on trimethylsilyl-controlled pore glass beads followed by reversed phase high pressure liquid chromatography. Recovery of IL-2 activity was greater than 100% of initial activity. The specific activity of the purified IL-2 ranged from 2 X 10(6) to 3.6 X 10(7) U/mg protein. Colony stimulating factor (CSF) and macrophage activating factor (MAF) were also separated on the chromatographic columns. This technique is useful for the purification of large quantities of these lymphokines which have potential in vivo applications.
D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003115:Colony-Stimulating Factors / Q000302:isolation & purification; D007376:Interleukin-2 / Q000302:isolation & purification; D008222:Lymphokines / Q000302:isolation & purification*; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D051381:Rats; D013945:Thymoma / Q000378:metabolism; D013953:Thymus Neoplasms / Q000378:metabolism; D014297:Trimethylsilyl Compounds
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D014297:Trimethylsilyl Compounds