Characterization of clostridia isolated from faeces of limited flora mice and their effect on caecal size when associated with germ-free mice.
115 strains of clostridia were accumulated from 3 separate isolations from the faeces of 1 limited flora (LF) mouse produced by inoculation of germ-free mice with chloroform-treated faeces of conventional mice. These strains were divided into 36 types on the basis of conventional biochemical characteristics. There was some variation in types isolated on the 3 occasions. Although 3 groups of polyassociated (PA) mice were produced from mixing 46 monoassociated mice, each inoculated with 1 of 46 strains of 36 bacterial types, the caecal size of PA mice was still greater than that of the control mice prepared by inoculation of chloroform-treated faeces of an LF mouse. After PA mice of each group were housed together, the caecal size became smaller and was only slightly larger than the control.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001431:Bacteriological Techniques; D002087:Butyrates / Q000378:metabolism; D002432:Cecum / Q000033:anatomy & histology*; D003013:Clostridium / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005561:Formates / Q000378:metabolism; D005856:Germ-Free Life; D008297:Male; D051379:Mice
D000085:Acetates; D002087:Butyrates; D005561:Formates