Use of non-human plasma for in vitro cultivation and antimalarial drug susceptibility testing of Plasmodium falciparum.
Viability, growth rate, and chemotherapeutic susceptibility of the CDC/Indochina III, CDC/Sierra Leone I, and FCR-3 (Subline F-86) isolates of Plasmodium falciparum grown continuously in RPMI 1640 medium supplemented with goat, horse, porcine, bovine, or ovine plasma were evaluated. Results were compared to those obtained from parallel cultures maintained in medium supplemented with non-immune human plasma. Only media supplemented with goat or horse plasma supported significant continuous multiplication of the isolates. Medium supplemented with either ovine or porcine plasma supported continuous multiplication of the CDC/Indochina III isolate, but not the FCR-3 isolate. Medium supplemented with bovine plasma did not support continuous growth of any of the isolates tested. The light microscopic appearance of the isolates during and after continuous culture in medium supplemented with either goat or horse plasma was identical to that of the control parasites maintained in medium supplemented with human plasma. There were no statistically significant differences in the susceptibility to antimalarial drugs of the culture lines maintained in medium supplemented with either human or goat plasma.
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D002417:Cattle / Q000097:blood; D002738:Chloroquine / Q000031:analogs & derivatives / Q000494:pharmacology; D003470:Culture Media; D006041:Goats / Q000097:blood; D006736:Horses / Q000097:blood; D006801:Humans; D015767:Mefloquine; D010949:Plasma; D010963:Plasmodium falciparum / Q000187:drug effects / Q000254:growth & development*; D011804:Quinolines / Q000494:pharmacology; D012756:Sheep / Q000097:blood; D013552:Swine / Q000097:blood
D000962:Antimalarials; D003470:Culture Media; D011804:Quinolines; C023676:chloroquine diphosphate; D002738:Chloroquine; D015767:Mefloquine