[Experience with long-term preservation of international-standard cultures of Actinomycetes].
The results of the study on long-term maintenance (1-12 years) of 453 reference cultures of actinomycetes are presented. The cultures were maintained with the following methods: lyophilization, soil cultures and periodical passages on agarized nutrient media. Lyophilization was most efficient for storage of the majority of the cultures: 346 strains (77.4 per cent) preserved high viability over the observation period of 11-12 years. Lyophilization appeared less favourable for the cultures with low sporulation. The efficacy of the method of soil cultures on long-term storage of the whorl actinomycetes was low: 88 per cent of Streptoverticillium lost their viability within 1-2 years. However, this method was valuable for stabilization of the taxonomic features of streptomycetes. The maintenance of the actinomycetes by regular passages on agarized media every 6 months resulted in the changing of the cultural and morphological features in a number of the strains.
D000192:Actinomycetales / Q000254:growth & development*; D000362:Agar; D001431:Bacteriological Techniques; D005612:Freeze Drying; D066298:In Vitro Techniques; D007391:International Cooperation; D011309:Preservation, Biological; D012015:Reference Standards; D012987:Soil; D013302:Streptomyces / Q000254:growth & development; D013997:Time Factors
D012987:Soil; D000362:Agar
Opyt dlitel'nogo khraneniia kul'tur mezhdunarodnykh vidovykh étalonov aktinomitsetov.