Activation of an interleukin-1-responsive T-cell lymphoma by fixed P388D1 macrophages and an antibody against the Ag:MHC T-cell receptor.
We have characterized the activation of the interleukin 1 (IL-1)-responsive T-cell lymphoma LBRM33-1A5-47 to interleukin 2 (IL-2) production by phytohemagglutinin (PHA) and IL-1. The signal provided by soluble IL-1 could be replaced by fixed P388D1 cells and the signal provided by PHA by a monoclonal antibody directed against an allotypic determinant of the Ag/MHC T-cell receptor (KJ16-133). The signal provided by fixed P388D1 does not appear to be due to alloreactivity. Instead, it appears to be due to a membrane-bound IL-1. The implications of these observations are discussed.
D000818:Animals; D002460:Cell Line; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007375:Interleukin-1 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008223:Lymphoma / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D051381:Rats / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects
D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D007375:Interleukin-1; D011948:Receptors, Antigen, T-Cell