title: Identification of a transcriptional enhancer element upstream from the proto-oncogene fos.
abstract: Sequences upstream from the proto-oncogene fos were shown to be essential for its transcription. Transient expression of the chloramphenicol acetyl-transferase (CAT) gene linked to upstream sequences of the fos gene including its promoter reveals that sequences located 64 to 404 base pairs 5' to the fos cap site contain a typical transcriptional enhancer. Moreover, these enhancer sequences, which are strikingly conserved between mouse and human fos genes, coincide with a deoxyribonuclease I-hypersensitive site in the chromatin. The expression of the fos-CAT fusion genes was stimulated only two to three times by the fos inducer 12-0-tetradecanoyl phorbol-13-acetate. The fos enhancer does not appear to be tissue-specific.
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase; D002843:Chromatin / Q000378:metabolism; D004274:DNA, Recombinant; D003850:Deoxyribonuclease I / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D006801:Humans; D051379:Mice; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004274:DNA, Recombinant; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D003850:Deoxyribonuclease I