The use of flow cytometry to measure X-ray survival in cultured T lymphocytes.
Light-scattering signals produced in a flow cytometer containing unstained, irradiated T-lymphocytes (MOLT-4 cell line) were analysed by plotting the axial light loss versus right-angle scatter. The resulting three-dimensional scattergram separated into two regions, corresponding to live and dead cells, as confirmed by trypan blue staining. The method is simple, rapid, allows large numbers of cells to be measured, avoids staining artifacts and is suitable for measuring radiation-induced killing down to 0.5 to 0.1 Gy.
D002460:Cell Line; D002470:Cell Survival; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D013601:T-Lymphocytes / Q000528:radiation effects*; D014343:Trypan Blue; D014965:X-Rays
D014343:Trypan Blue