Effects of diacetyl diamines on in vitro activation and proliferation of human B lymphocytes.
N,N'-Diacetylputrescine (tetramethylenebisacetamide [TMBA]) and its six carbon analog, hexamethylenebisacetamide (HMBA), inhibited the proliferative response of human B lymphocytes to anti-mu and formalinized Cowan I strain Staphylococcal aureus (SAC) stimulation. In contrast, B cell growth factor-stimulated proliferation of human B cells was minimally inhibited by TMBA or HMBA. The antiproliferative effect of these diamine derivatives was specific for anti-mu (or SAC) activation of normal B cells, because the proliferation of PHA-stimulated human T cells and transformed human B cells was not affected by the presence of TMBA or HMBA. The inhibitory effect of diacetyl diamines on anti-mu (or SAC)-induced B cell activation was dose dependent and persisted after removal of the diamine derivatives from the culture media. These studies show that diacetylated derivatives of polyamines modulate human B cell activation in vitro by specific abrogation of anti-mu or SAC activation.
D000081:Acetamides / Q000494:pharmacology*; D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D006133:Growth Substances / Q000502:physiology; D006801:Humans; D007075:Immunoglobulin M / Q000502:physiology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D015847:Interleukin-4; D008213:Lymphocyte Activation / Q000187:drug effects*; D008222:Lymphokines / Q000502:physiology; D008940:Mitotic Index / Q000187:drug effects; D011700:Putrescine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000276:immunology
D000081:Acetamides; D000888:Antibodies, Anti-Idiotypic; D006133:Growth Substances; D007075:Immunoglobulin M; D007166:Immunosuppressive Agents; D008222:Lymphokines; C044203:anti-IgM; D015847:Interleukin-4; C026212:N-acetylputrescine; C014026:hexamethylene bisacetamide; D011700:Putrescine