Effect of haemopoietic stem-cell proliferation regulators on early and late spleen colony-forming cells.
An inhibitor and stimulator of CFU-s proliferation can be obtained from haemopoietic tissue containing, respectively, relatively quiescent CFU-s (e.g. normal bone marrow) and proliferating CFU-s (e.g. regenerating bone marrow). Their effects on the proliferative behaviour of steady-state and regenerating marrow CFU-s, which produce colonies 7, 10 and 12 days post-transplantation have been investigated. The results demonstrate changing sensitivities of CFU-s to inhibitor and stimulator as they progress through a developmental age structure, 'Older' CFU-s (producing early spleen colonies) are more sensitive to stimulator, 'Younger' CFU-s (producing late spleen colonies) are more sensitive to inhibitor.
D000818:Animals; D001853:Bone Marrow / Q000032:analysis; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005260:Female; D006133:Growth Substances / Q000032:analysis / Q000494:pharmacology*; D016298:Hematopoietic Cell Growth Factors; D008222:Lymphokines / Q000032:analysis / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010659:Phenylhydrazines / Q000494:pharmacology; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000187:drug effects*; D013997:Time Factors
D003470:Culture Media; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D008222:Lymphokines; D010659:Phenylhydrazines; C022173:stem cell inhibitory factor; C030299:phenylhydrazine