title: Production and regeneration of protoplasts from Cryptococcus.
abstract: Protoplasts were quickly and efficiently produced from both varieties of Cryptococcus neoformans and from C. laurentii by use of the multi-enzyme product Novozym 234. Conditions for regeneration of protoplasts are described. DNA yield from the Novozym-produced protoplasts was superior to that from snail gut enzyme-derived protoplasts.
mesh_terms: D003454:Cryptococcus / Q000187:drug effects*; D003455:Cryptococcus neoformans / Q000187:drug effects; D004798:Enzymes; D005966:Glucuronidase / Q000494:pharmacology; D008828:Microbiological Techniques; D009097:Multienzyme Complexes / Q000494:pharmacology; D011523:Protoplasts; D013429:Sulfatases / Q000494:pharmacology
chemical_list: D004798:Enzymes; D009097:Multienzyme Complexes; C023059:glusulase; C045154:novozym 234; D013429:Sulfatases; D005966:Glucuronidase