Presence of tropomyosin in adrenal chromaffin cells and its association with chromaffin granule membranes.
The presence of tropomyosins in adrenal chromaffin cells was demonstrated by immunoperoxidase staining of cultured chromaffin cells by an anti-tropomyosin antibody. In immunoblotting the antibody labelled polypeptides of Mr 39 000, 36 500 and 31 000 in 100 000 X g supernatants and heat-stable fractions of adrenal medulla. The adrenal medullary tropomyosins did not comigrate with smooth muscle tropomyosins. The 39-kDa and 31-kDa polypeptides comigrated with polypeptides labelled by anti-tropomyosin in brain supernatants. Chromaffin granule membranes possessed a 39-kDa polypeptide that was labelled by anti-tropomyosin.
D000313:Adrenal Medulla / Q000648:ultrastructure*; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000648:ultrastructure*; D002838:Chromaffin System / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008970:Molecular Weight; D014335:Tropomyosin / Q000032:analysis*
D014335:Tropomyosin