Double labeling of human leukemic cells using 3H-cytarabine and monoclonal antibody against bromodeoxyuridine.
A new technique using immunofluorescence and autoradiography is described, in which the DNA of cells in S phase are labeled with two different probes. This method makes it possible to study the relationship between DNA synthesis and the uptake and/or incorporation of chemotherapeutic agents into normal or neoplastic cells. An example is provided in which the incorporation of 3H-cytarabine into DNA is demonstrated to occur only in cells which were synthesizing DNA during exposure to 3H-cytarabine. Other radioactively labeled probes can be used as well.
D000911:Antibodies, Monoclonal; D001345:Autoradiography / Q000379:methods; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003561:Cytarabine / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014410:Tumor Stem Cell Assay
D000911:Antibodies, Monoclonal; D003561:Cytarabine; D014316:Tritium; D004247:DNA; D001973:Bromodeoxyuridine; D013936:Thymidine