Sulfur uptake by type I collagen from methyl mercaptan/dimethyl disulfide air mixtures.
Type I acid-soluble collagen, suspended in 0.02 M Tris/0.13 M NaCl buffer (pH 7.4), was exposed to air atmosphere admixed with 10.7 X 10(-9) moles of [35S]-labeled dimethyl disulphide/methyl mercaptan mixture in the ratio of 99.82% (CH3S)2/0.18% CH3SH. Gas chromatographic analyses of head-space following one and four days of incubation indicated that all of the CH3SH was absorbed from the head-space by the collagen-containing liquid phase, while the (CH3S)2 concentration of the head-space remained essentially unchanged. Of the total [35S]-activity initially present in the head-space, only 1.0 the total [35S]-activity initially present in the head-space, only 1.0 and 1.86% was absorbed by the collagen-containing liquid phase within one and four days, respectively. Paper chromatographic separation of the reaction mixture showed that only 0.42 and 0.60% of the radioactivity in the head-space was associated with collagen after one and four days of reaction. In comparable [35S]-H2S systems devoid of (CH3S)2, all of the H2S was absorbed within 48 hours of incubation with 18.04% (1 day) and 18.28% (4 days) of the available [35S]-H2S complexed with collagen. The results provide evidence that the reaction of collagen with H2S and CH3SH/(CH3S)2 mixture proceeded via the H2S and CH3SH thiol groups.
D000818:Animals; D002849:Chromatography, Gas; D003094:Collagen / Q000378:metabolism*; D004220:Disulfides; D009812:Odorants; D051381:Rats; D012965:Sodium Chloride; D012997:Solvents; D013438:Sulfhydryl Compounds; D013455:Sulfur / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013997:Time Factors; D014325:Tromethamine
D004220:Disulfides; D012997:Solvents; D013438:Sulfhydryl Compounds; D013462:Sulfur Radioisotopes; D014325:Tromethamine; C005231:methylmercaptan; C021181:dimethyl disulfide; D012965:Sodium Chloride; D013455:Sulfur; D003094:Collagen