title: MgATP specifically controls in vitro self-assembly of vertebrate skeletal myosin in the physiological pH range.
abstract: The appearances in the electron microscope of rat and rabbit skeletal muscle myosin filaments and rod aggregates, formed in the presence of variable amounts of MgATP, were compared at different pH values. It is shown that small amounts of MgATP, similar to those sufficient to trigger the dissociation of the actomyosin complex, were able to modify the geometry of myosin filaments profoundly in the physiological pH range, whereas the conformation of rod aggregates remained unchanged even in the presence of high concentrations of MgATP. Myosin filaments formed in the absence of MgATP displayed the classical spindle-shaped conformation and variable diameters at all pH values, whereas myosin filaments formed in the presence of MgATP in the physiological pH range had constant diameters, similar to those of natural thick filaments. These filaments of constant diameter frayed, rapidly and reversibly, into two types of subfilaments with respective diameters of 4 to 5 nm and 9 to 10 nm, when the pH of the medium was raised above 7.2. Spindle-shaped myosin filaments and rod aggregates remained unchanged by such small changes in pH. It was possible to change the conformation of preformed spindle-shaped filaments by simply adding MgATP to the medium, but this reaction was slow and took several hours to be completed. Relatively high concentrations of MgATP, similar to those in the living cell, increased the solubility of both myosin filaments and rod aggregates in the alkaline pH range (pH greater than or equal to 7.0). Low pH values (less than or equal to 6.5) and excess free Mg2+ (greater than or equal to 6 to 7 mM) abolished both the specific effect of MgATP on myosin filament conformation and its solubilizing effect on both myosin filaments and rod aggregates. The degree of purity of the myosin preparations and the level of phosphorylation of the LC-2 light chains did not influence filament behaviour noticeably and rat and rabbit myosins behaved similarly.
mesh_terms: D000255:Adenosine Triphosphate; D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008274:Magnesium; D015636:Magnesium Chloride; D008854:Microscopy, Electron; D009132:Muscles / Q000032:analysis*; D009218:Myosins; D011817:Rabbits; D051381:Rats
chemical_list: D046911:Macromolecular Substances; D015636:Magnesium Chloride; D000255:Adenosine Triphosphate; D009218:Myosins; D008274:Magnesium