Erythrocyte-bound immune complexes trigger the release of interleukin-1 from human monocytes.
We have demonstrated herein that immune complexes (IC) bound to O+ human erythrocytes trigger the release of interleukin-1 (IL-1) from human monocytes in vitro. The efficiency of monocytes stimulation by erythrocyte-bound IC is in sharp contrast to the lack of stimulation of monocytes by soluble IC. The IL-1 activity triggered by erythrocyte-bound IC was lower than that induced by insoluble IC or opsonized fluorescent latex beads, but was greater than that induced by anti-D-treated O+ human erythrocytes. The magnitude of IL-1 release induced by insoluble IC and erythrocyte-bound IC were similar up to 18 hr of incubation. The IL-1 activity decreased in the supernatant of monocytes exposed to erythrocyte-bound IC for 40 hr, while the activity of IL-1 induced by insoluble IC remained at a high level. The physiological and physiopathological significance of triggering IL-1 release by erythrocyte-bound IC are discussed.
D000017:ABO Blood-Group System; D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism / Q000502:physiology*; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D051379:Mice; D008809:Mice, Inbred C3H; D009000:Monocytes / Q000378:metabolism*; D011817:Rabbits; D013745:Tetanus Toxoid / Q000276:immunology; D013997:Time Factors
D000017:ABO Blood-Group System; D000936:Antigen-Antibody Complex; D007375:Interleukin-1; D013745:Tetanus Toxoid