[Histamine receptor-bearing lymphocytes. III. Suppression of immune reactions by killing of histamine receptor-bearing lymphocytes with a conjugate of histamine and the A chain of mistletoe lectin I].
Conjugates of the A-chain of the mistletoe lectin I and histamine but not A-chain per se inhibit the capacity of spleen cells of the mouse to induce antibody response or graft-versus-host reaction by 90%. The conjugates killed 27% of the spleen cells, 60% of the T-, and 15% of the B-lymphocytes. The receptors for histamine could be shown to be of the H2-type, because only H2-antagonists inhibited the toxic influence of the conjugates. The results suggest the hypothesis that not only suppressor cells but at least the majority of the immunocompetent cells bear receptors for histamine. The A-chain-histamine conjugate represents an "affinotoxin" which is cytotoxic only after affinity binding on receptors of the target cell.
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001665:Binding Sites; D003602:Cytotoxicity, Immunologic; D006087:Graft vs Host Reaction; D006632:Histamine / Q000031:analogs & derivatives* / Q000276:immunology; D007166:Immunosuppressive Agents; D066298:In Vitro Techniques; D037102:Lectins / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D028321:Plant Preparations; D010940:Plant Proteins; D011968:Receptors, Histamine / Q000378:metabolism*; D011970:Receptors, Histamine H2 / Q000276:immunology / Q000378:metabolism*; D054790:Ribosome Inactivating Proteins, Type 2; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014118:Toxins, Biological / Q000276:immunology*
D007166:Immunosuppressive Agents; D037102:Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D011968:Receptors, Histamine; D011970:Receptors, Histamine H2; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum; D006632:Histamine
Histaminrezeptor-tragende Lymphozyten. III. Suppression von Immunreaktionen nach Abtöten Histaminrezeptor-tragender Lymphozyten durch ein Konjugat aus Histamin und der A-Kette des Mistellektins I.