[Karyological analysis of hybridoma lines producing monoclonal antibodies to viral antigens].
The number of Hybridomes obtained from various sources rapidly increases at present. Clones producing monoclonal antibodies to influenza virus A/USSR/090/77 and to VEE-230 are generated in the laboratory of the Institute of Virology (Academy of Sciences of the USSR). The present work is devoted to the study of Hybridome karyotype by means of C-method for chromosome staining with the aim to reveal specific characteristics of these lines. Results of the investigation have shown that the count of chromosomes together with an examination of their C-staining picture permit proving a hybrid nature of the clones and identifying various Hybridomes by chromosome markers.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002871:Chromosome Banding; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology; D005819:Genetic Markers; D006825:Hybridomas / Q000276:immunology / Q000648:ultrastructure*; D007114:Immunization; D009980:Influenza A virus / Q000276:immunology*; D007621:Karyotyping; D008677:Metaphase; D051379:Mice
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D005819:Genetic Markers
Kariologicheskiĭ analiz gibridomnykh liniĭ, produtsiruiushchikh monoklonal'nye antitela virusnym antigenam.