Growth of normal human T lymphocytes induced by monoclonal antibody to the T cell antigen receptor.
A monoclonal antibody (mAb) called 30-3D6 has been raised against the T cell antigen receptor analogue on a human T cell leukemia cell line HPB-ALL. This mAb comodulates the T3 molecule on HPB-ALL and precipitates the heterodimeric structure previously described as a T cell idiotypic receptor analogue on this cell line. 30-3D6 reacts with a variable percentage of normal T cells (up to 6%) depending on the donor and this number is stable on repeated sampling and is not affected by the temperature of the reaction. When normal T cells from a high frequency donor are stimulated with 30-3D6 and interleukin 2 in vitro the idiotype-positive (Id+) population can be expanded. Large numbers of greater than 90% Id+ T cells can be generated. Id cells are present in both the helper and cytotoxic suppressor subsets.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000937:Antigen-Antibody Reactions; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000502:physiology; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D008934:Mitogens; D011948:Receptors, Antigen, T-Cell