The effect of sorbic acid and esters of p-hydroxybenzoic acid on the protonmotive force in Escherichia coli membrane vesicles.
The effect of three food preservatives, sorbic acid and methyl and butyl esters of p-hydroxybenzoic acid, on the protonmotive force in Escherichia coli membrane vesicles was investigated. Radioactive chemical probes were used to determine the two components of the protonmotive force: delta pH (pH difference) and delta psi (membrane potential). Both types of compound selectively eliminated delta pH across the membrane, while leaving delta psi much less disturbed indicating that transport inhibition by neutralization of the protonmotive force cannot be the only mechanism of action for the food preservatives tested.
D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000648:ultrastructure; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D010226:Parabens; D013011:Sorbic Acid / Q000494:pharmacology*
D005231:Fatty Acids, Unsaturated; D062385:Hydroxybenzoates; D010226:Parabens; C038193:4-hydroxybenzoic acid; D013011:Sorbic Acid