The identification of several prostaglandin moieties in Crassostrea virginica and Mytilus edulis by radioimmunoassay and high performance liquid chromatography.
The presence of prostaglanin-like compounds was examined by radioimmunoassay and isochratic step-elution high performance liquid chromatography in two commercially important molluscs, the American oyster Crassostrea virginica, and the edible mussel Mytilus edulis. Radioimmunoassays for prostaglandins F2 alpha and E2, and 6-keto F1 alpha (the stable hydrolysis product of prostacyclin) demonstrated mean +/- S.D. levels of 3.19 +/- 2.1, 13.08 +/- 5.4, and 1.5 +/- 0.42 nanograms of prostaglandin equivalents, respectively, in C. virginica extracts. Levels of PGF2 alpha, PGE2, and 6-keto PGF1 alpha were 4.0 +/- 2.2, 33.34 +/- 3.5, and 7.18 +/- 2.3, respectively, in M. edulis samples. Chromatographic analyses of organic extracts of these bivalues demonstrated the presence of one or more moieties possessing retention times similar to, or identical with, standards of PGF2 alpha, PGE2, and 6-keto PGF1 alpha. The immunological and chromatographic data, as well as the polyenoic fatty acid profiles of these species, suggest the presence of immunoactive prostanoids of the 2- and 3-series. These findings are discussed in the context of present knowledge of eicosanoid biochemistry and physiology in marine invertebrates.
D000818:Animals; D049872:Bivalvia / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010125:Ostreidae / Q000032:analysis*; D011453:Prostaglandins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D013045:Species Specificity; D014316:Tritium
D011453:Prostaglandins; D014316:Tritium