The karyotype of the glucocorticoid-sensitive, lymphoblastic human T-cell line CCRF-CEM shows a unique deleted and inverted chromosome 9.
Chromosome karyotypes were prepared from the highly glucocorticoid-sensitive clone C7 of the human acute lymphoblastic leukemia T-cell line CCRF-CEM. The modal number of chromosomes is 47, and one chromosome #9 has a pericentric inversion of the heterochromatin region (9qh) plus a deletion of the short arm. In most cells, there is an extra chromosome #20. All other chromosomes appear to be normal. Examination of the uncloned line CCRF-CEM (ATCC CCL 119), which was frozen away shortly after the line was originated and has undergone fewer passages than CEM C7, also revealed the same abnormality of chromosome #9. Each of 10 other clones isolated from CCRF-CEM in this laboratory also contained the abnormal chromosome #9.
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002871:Chromosome Banding; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D002906:Chromosomes, Human, 6-12 and X; D002999:Clone Cells; D004351:Drug Resistance; D005938:Glucocorticoids / Q000633:toxicity*; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000235:genetics*; D013601:T-Lymphocytes
D005938:Glucocorticoids