title: In vitro cultivation of Babesia bigemina.
abstract: A strain of Babesia bigemina was isolated from an infected calf and propagated in vitro. Culture conditions included washing of infected and normal bovine erythrocytes in a special solution, and the use of a 5% to 10% (v/v) erythrocyte suspension in medium 199 (with 20% to 50% fresh normal bovine serum) at a depth of 4 mm in a 5% CO2, 2% O2, 93% N2 atmosphere. After 36 days in vitro and 9 subcultures, the cultured organism was inoculated into a susceptible calf. This calf developed clinical signs of disease and recovered when treated with 1% trypan blue solution. The strain was also reisolated from the second calf. The original isolate had been maintained in continuous in vitro cultivation for more than 99 days.
mesh_terms: D000818:Animals; D001403:Babesia / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D001404:Babesiosis / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D010100:Oxygen
chemical_list: D003470:Culture Media; D010100:Oxygen