Cytogenetic and DNA-flow cytometric studies of separated blasts.
With Percoll density gradients, blasts from peripheral blood and bone marrow could be separated with a significant enrichment, and very often with a high degree of purity. This allowed a study of selected cases, where the separated sample exhibited chromosome abnormalities and/or an abnormal DNA content distribution (as measured by DNA-flow cytometry). The anomalies were shown to be associated with the separated blast fraction.
D001853:Bone Marrow / Q000648:ultrastructure; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics*; D055728:Primary Myelofibrosis / Q000235:genetics*
D004247:DNA