Mutation induction by excess deoxyribonucleotides in Saccharomyces cerevisiae.
Excess dTMP is toxic and mutagenic with exponentially growing dTMP efficient uptaking yeast strains 831 rho+ and 833 rho. The respiratory deficient strain 833 exhibits a tenfold sensitivity to the genotoxicity of excess dTMP. Mutant yield in the forward mutation system CAN1----can1 after dTMP excess is comparable to that found after irradiation with UV254nm. Excess dTMP is a poor mutagen in stationary phase cells of both strains. Mutagenicity of excess dTMP is not found in an ochre mutant allele (ade2-1). Exposure of exponentially growing cells to other deoxyribonucleotides (dCMP, dAMP, and dGMP) reveal these nucleotides to have mutagenic potential as well.
D002172:Canavanine / Q000494:pharmacology; D003838:Deoxyadenine Nucleotides / Q000494:pharmacology; D003843:Deoxycytidine Monophosphate / Q000494:pharmacology; D003848:Deoxyguanine Nucleotides / Q000494:pharmacology; D003854:Deoxyribonucleotides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013938:Thymidine Monophosphate / Q000494:pharmacology
D003838:Deoxyadenine Nucleotides; D003848:Deoxyguanine Nucleotides; D003854:Deoxyribonucleotides; D003843:Deoxycytidine Monophosphate; D013938:Thymidine Monophosphate; D002172:Canavanine; C007257:2'-deoxyguanosine 5'-phosphate