[An immunoenzyme method of determining benzylpenicillin in biological substrates and its antibodies in immunized animals].
Lately it appeared to be necessary to develop-alternative methods for control of antibiotic therapy. Detection of low molecular substances based on the immunoenzymatic assay (IEA) is one of the most promising approaches to the development of such methods. The authors developed a quantitative method for determination of benzylpenicillin (BP) according to the procedure described earlier by Van Weemen. The BP antisera were prepared by immunization of rabbits with a conjugate of bovine serum albumin. The gamma-globulin fraction isolated from the antisera was conjugated with peroxidase. The working dilution of the conjugate was determined by the chess titration method. The test system of competitive binding developed by the authors provides detection of BP in the biological fluids in concentrations of 0.1 to 0.0001 microgram/ml (by BP dry weight).
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001667:Binding, Competitive; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D010400:Penicillin G / Q000097:blood* / Q000276:immunology; D011817:Rabbits
D000906:Antibodies; D007106:Immune Sera; D010400:Penicillin G
Immunofermentnyĭ metod dlia opredeleniia benzilpenitsillina v biologicheskikh substratakh i antitel k nemu u immunizirovannykh zhivotnykh.