[In vitro synthesis of interleukin 2 (IL-2) by T lymphocytes extracted from a lymphoepithelial thymoma. Purification and biochemical characterization].
T (E+) lymphocytes purified from lymphoepithelial thymoma surgically removed from an asymptomatic 12 yrs old girl were cultured in presence of PHA-P; culture supernatant contained a T cell growth factor (IL-2) biochemically different from that purifiable from supernatants of normal T cells. Differences in molecular weight, isoelectric point and ion exchange elution pattern would allow to compare this abnormal IL-2, (named Thy-IL-2 from Thymus) to that already characterized from the HUT 102 T cell line (L-TCGF peak II). A comparison of biochemical properties of Thy-IL-2 and normal IL-2 (N-IL-2) is also presented; the biological significance of such differences are discussed.
D002648:Child; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000302:isolation & purification; D007525:Isoelectric Focusing; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000378:metabolism*; D013945:Thymoma / Q000276:immunology*
D007376:Interleukin-2
Sintesi di interleuchina 2 (IL-2) in vitro da parte di T-linfociti estratti da timoma linfoepiteliale. Purificazione e caratterizzazione biochimica.