title: Induction of umuC gene expression by nitrogen dioxide in Salmonella typhimurium.
abstract: Gaseous nitrogen dioxide (NO2) was found to induce umuC gene expression in Salmonella typhimurium carrying the umuC-lacZ fusion plasmid. The induction level of the umu operon responsible for inducible mutagenesis was measured by the level of beta-galactosidase in the cell, encoded by the fusion gene. NO2 gas was bubbled into bacterial suspensions at 10, 30 and 90 microliters/l for 30 min at a flow rate of 100 ml/min. Expression of the umuC gene varied with the concentration, flow rate and bubbling time of the NO2 gas. Although NO2 gas induces SOS functions, mutagenesis due to it was not detectable in Salmonella typhimurium TA100 and TA102. Nitric oxide gas (NO) did not induce any umuC gene expression.
mesh_terms: D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial; D009154:Mutation / Q000187:drug effects*; D009585:Nitrogen Dioxide / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D009585:Nitrogen Dioxide