Stable cavitation at low ultrasonic intensities induces cell death and inhibits 3H-TdR incorporation by Con-A-stimulated murine lymphocytes in vitro.
Murine spleen cell suspensions stimulated by Concanavalin A (Con-A) were exposed to 1.6-MHz continuous-wave ultrasound at low intensities (spatial-peak values ranging from 16 to 300 mW/cm2) in the presence of a Nuclepore membrane that contained stabilized gas bodies. The ultrasonically activated gas bodies induced cell lysis and reduced the fraction of intact cells that excluded trypan blue. At a spatial-peak intensity of 75 mW/cm2 (spatial-average intensity 15 mW/cm2), Con-A-induced Methyl[3H]thymidine (3H-TdR) incorporation was reduced in cultures exposed at 24 or 48 hr after addition of Con-A but not at 7 or 13 hr. There was no observable effect on cell survival or 3H-TdR incorporation at spatial-peak intensities below 75 mW/cm2.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002470:Cell Survival; D003208:Concanavalin A / Q000276:immunology; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology*; D013936:Thymidine / Q000031:analogs & derivatives / Q000378:metabolism; D014465:Ultrasonics / Q000009:adverse effects*
D003208:Concanavalin A; D000255:Adenosine Triphosphate; C012439:methylthymidine; D013936:Thymidine