A convenient protein substrate for the determination of protease specificity: reduced and S-3-(trimethylated amino)propylated lysozyme.
Reduced lysozyme was alkylated with 3-bromopropyltrimethylammonium bromide to give reduced and S-3-(trimethylated amino)propylated lysozyme. It was soluble in a wide range of pH and is suitable as the protein substrate to determine protease specificity.
D000478:Alkylation; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D009113:Muramidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010447:Peptide Hydrolases / Q000378:metabolism*; D011489:Protein Denaturation; D000644:Quaternary Ammonium Compounds; D013379:Substrate Specificity; D014357:Trypsin / Q000378:metabolism
D000644:Quaternary Ammonium Compounds; C045443:3-bromopropyltrimethylammonium bromide; D009113:Muramidase; D010447:Peptide Hydrolases; D014357:Trypsin