Cross-reactivity between solid-phase immunoassay plates and intermediate filaments demonstrated by human monoclonal antibodies.
While screening supernatants of human-human hybridomas for rheumatoid factor and anti-cellular activity we found that a significant number of supernatants which react with the Falcon-polyvinyl chloride immunoassay plate used in an enzyme-linked immunosorbent rheumatoid factor assay also react with intracellular intermediate filaments.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D003429:Cross Reactions; D003599:Cytoskeleton / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007163:Immunosorbent Techniques; D007381:Intermediate Filament Proteins / Q000276:immunology*; D010969:Plastics
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D010969:Plastics