Demonstration of structural polymorphism among MB3 light chains by two-dimensional gel electrophoresis.
The heavy and light chain subunits of MB3 molecules were isolated from KT2 (DKT2, DR4, MB3 homozygous), ER (Dw4, DR4, MB3 homozygous), JMe (Dw5, DR5, MB3 homozygous), EBV-Sh (DSh, DRw6.2, MB3 homozygous), and EBV-Ky (DKy, DRw9, MB3 homozygous) cells and were compared with one another by two-dimensional gel electrophoresis. The MB3 light chains from KT2, ER, and EBV-Ky cells were clearly different in terms of their isoelectric points, whereas those from ER, JMe, and EBV-Sh cells were indistinguishable. No differences in charge or m.w. were noted for the MB3 heavy chains from the five cell lines. Thus, three out of the five MB3-positive, D/DR-disparate cell lines were found to express structurally distinct MB3 molecules, demonstrating that MB3 is a public serologic specificity shared by at least three structurally distinct MB (human I-A-like) molecules. Because the DR light chain subunits isolated from EBV-Wa, KT2, ER, JMe, EBV-Sh, and EBV-Ky cells differed from one another in their isoelectric points, the DR light chains were apparently more polymorphic than the MB3 light chains.
D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006680:HLA Antigens / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000302:isolation & purification; D006720:Homozygote; D006801:Humans; D007519:Isoantigens / Q000235:genetics* / Q000302:isolation & purification; D007945:Leukemia, Lymphoid / Q000276:immunology; D046911:Macromolecular Substances; D010641:Phenotype; D011110:Polymorphism, Genetic
D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D046911:Macromolecular Substances