Effect of membrane potential on the kinetic parameters of the Na+ or H+ melibiose symport in Escherichia coli membrane vesicles.
Comparison of the transport properties of the melibiose permease of E. coli acting as a H+-symport or a Na+-symport has been performed by measuring initial rates of [3H]-melibiose transport or its accumulation in isolated membrane vesicles. The results show strikingly that although the membrane potential primarily drives melibiose accumulation by both types of symport, it selectively affects the apparent affinity constant Kt of the H+-melibiose symport while it specifically changes the maximal rate of transport (Vmax) of the Na+-melibiose symport. It is suggested that modification(s) of some partial reaction constants of a given transport cycle might lead to important changes in the kinetic properties of this transport system.
D001693:Biological Transport, Active / Q000187:drug effects; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004187:Disaccharides / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D005374:Filtration; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D008553:Melibiose / Q000378:metabolism*; D008564:Membrane Potentials; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008985:Monensin / Q000494:pharmacology; D009550:Nigericin / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D027981:Symporters; D014634:Valinomycin / Q000494:pharmacology
D004187:Disaccharides; D026901:Membrane Transport Proteins; D027981:Symporters; D014634:Valinomycin; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D006859:Hydrogen; C021548:melibiose permease; D008985:Monensin; D008553:Melibiose; D012964:Sodium; D009550:Nigericin