An investigation into the effect of reagent volume on the spin indirect antiglobulin test.
Three polyspecific antiglobulin reagents were tested in a spin antiglobulin technique with 51 antibodies of varying blood group specificities in which the volume of antiglobulin reagent used ranged from 1 volume (35 microliters) to 3 volumes (105 microliters). Statistical analysis of results showed that a significant decrease in avidity and agglutination scores occurred as the volume of antiglobulin reagent used was increased ('volume effect'). The volume effect was shown by 33/51 (65%) of antibodies with all three antiglobulin reagents and by 48/51 (94%) of antibodies with at least one antiglobulin reagent. Only 3/51 (6%) failed to show the volume effect with all three antiglobulin reagents.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000915:Antibody Affinity; D003298:Coombs Test; D006801:Humans; D007202:Indicators and Reagents
D000888:Antibodies, Anti-Idiotypic; D007202:Indicators and Reagents