A gene probe for TEM type beta-lactamases.
A restriction fragment of plasmid pBR322 bearing the TEM-1 beta-lactamase structural gene was electroeluted from agarose gels after digestion with EcoRI and HinfI. The 1-kilobase fragment was 32P-labeled and used to examine genetic relationships with nucleic acids encoding seven other beta-lactamase classes. The probe hybridized only with TEM-2 and OXA-2 class plasmids.
D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005796:Genes; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1