T-cell subsets in lesions of systemic and discoid lupus erythematosus.
In 6 patients with untreated systemic lupus erythematosus (SLE) in the progressive stage, and in 6 with discoid lupus erythematosus (DLE), an analysis of inflammatory infiltrates was performed in situ using the avidin-biotin-peroxidase complex (ABC) method with monoclonal antibodies. In all patients, over 75% of the infiltrates reacted with the pan T-cell antibody OKT3, but only sporadically with that of B-cell OKB7. In addition, a large number of the infiltrates were OKIal-positive, indicating that they were in an activated state. Many OKT8-positive cells were seen infiltrating the epidermis especially in the vicinity of basal keratinocytes. Staining for T-cell subsets revealed that the proportion of OKT8-positive cells (suppressor/cytotoxic) was from 2 to 3 fold higher than that of OKT4-positive cells (helper/inducer) in lesions of SLE. On the contrary, in DLE, a predominance of OKT4-positive cells (the OKT4/OKT8 ratio was from 1:1 to 3:1) was observed. Thus, our results provide further evidence that these 2 main types of LE show quite contrary findings on immunohistochemical analysis of T-cell subsets, and that besides the humoral immune mechanism, the cell-mediated immune mechanism may be involved in the pathogenesis of these disorders.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D005260:Female; D006801:Humans; D008179:Lupus Erythematosus, Discoid / Q000276:immunology / Q000473:pathology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000473:pathology*; D008297:Male; D013601:T-Lymphocytes / Q000145:classification* / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface