A method for the accurate determination of the specificity of monoclonal antibodies reactive with porcine immunoglobulins.
Monoclonal antibodies against porcine IgG were produced by fusion and characterized. The supernatants of microtiter wells containing fusion hybrids were first screened with an ELISA using semi-purified porcine IgG as antigen. Hybrids reactive in ELISA were cloned by limiting dilution. Further characterization of the specificity of the monoclonal antibodies was done by a combination of two methods: SDS-PAGE electroimmunoblotting and convection blotting of immunoelectrophoretic patterns (IEP-immunoblotting). Using these techniques, we identified monoclonal antibodies specific for porcine Ig gamma chains.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D013552:Swine
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D003101:Collodion