title: Assignment of the gene encoding cytosolic phosphoenolpyruvate carboxykinase (GTP) to Mus musculus chromosome 2.
abstract: The structural gene Pck-1, encoding cytosolic phosphoenolpyruvate carboxykinase (GTP) (PEPCK; EC 4.1.1.32), has been assigned to mouse chromosome 2. This assignment was made based on genomic Southern transfer of rat-mouse and hamster-mouse hybrid DNAs using a rat kidney cloned cDNA of the PEPCK gene as a probe. Conclusive evidence for the cosegregation of the PEPCK gene with mouse chromosome 2 was obtained using a monochromosomal microcell hybrid that selectively retained a Robertsonian translocation between mouse autosomes 2 and 8 and its back-selected hybrid clone.
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure*; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D003600:Cytosol / Q000201:enzymology; D005796:Genes; D006822:Hybrid Cells / Q000201:enzymology; D051379:Mice; D009693:Nucleic Acid Hybridization; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D014176:Protein Biosynthesis
chemical_list: D010729:Phosphoenolpyruvate Carboxykinase (GTP)