PMN chemotactic factor produced by glass-adherent cells in the acute inflammation caused by Paracoccidioides brasiliensis.
Intraperitoneal inoculation of BIO.A mice with P. brasiliensis induces an acute inflammatory infiltrate in which 40-50% of the cells are PMN leucocytes. Previous depletion of serotonin, prostaglandin, histamine and complement does not alter the course of inflammation. Complement-derived factors appear to have no active participation in the process since C5-deficient mice depleted or not by Cobra venom factor (CoF) show the same kind of cellular influx. On the other hand, peritoneal cells incubated (6 h) with the fungus release a soluble factor that induces in vivo an active chemotaxis of PMN cells when inoculated i.p. The factor has the following characteristics: a) it is produced by adherent cells; b) it is protein in nature; c) its production is inhibited by incubation of peritoneal cells with 10 micrograms/ml puromycin and d) it has a molecular weight less than 15 000 daltons, as determined by gel filtration through a Sephadex G-75 column.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D002448:Cell Adhesion; D002630:Chemotactic Factors / Q000032:analysis / Q000096:biosynthesis*; D002634:Chemotaxis, Leukocyte; D003182:Complement C5 / Q000172:deficiency; D005260:Female; D016209:Interleukin-8; D007958:Leukocyte Count; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009504:Neutrophils / Q000276:immunology; D010229:Paracoccidioidomycosis / Q000276:immunology / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology
D002630:Chemotactic Factors; D003182:Complement C5; D016209:Interleukin-8; D011691:Puromycin