[Stereological analysis of phagocytosing cells].
Various complexes of phagocytes with adsorbed (I) and entrapped (J) particles are formed during phagocytosis. Method of stereological reconstruction is proposed that allows to demonstrate the actual distribution of these complexes on the basis of morphometric analysis of their ultrathin sections. The principle of the method lies on the probability simulation of section distribution for a given distribution of complexes and on the solution of the reverse problem by stepwise determination of the relative quantity of each complex type (from the most complicated to the most simple one, when I = 0 and J = 0). The stereological analysis of phagocytosing murine peritoneal macrophages revealed an absolutely different and more adequate kinetical picture of phagocytosis, as compared to the morphometric data.
D000042:Absorption; D000327:Adsorption; D000818:Animals; D002176:Candida albicans; D007700:Kinetics; D008264:Macrophages / Q000276:immunology / Q000648:ultrastructure; D008433:Mathematics; D051379:Mice; D008808:Mice, Inbred CBA; D008954:Models, Biological; D010587:Phagocytosis; D013997:Time Factors
Stereologicheskiĭ analiz fagotsitiruiushchikh kletok.