DNA-fork displacement rates in cultured mammalian cells with mutations in ribonucleotide reductase.
DNA-replication fork displacement rates were measured in mouse S49 lymphosarcoma cell lines and in derivatives of those cell lines. One of the derivatives lacks dCMP deaminase activity and two others bear defined mutations in ribonucleotide reductase. We also examined a revertant cell line that was selected from one of the ribonucleotide reductase mutants and has regained normal ribonucleotide reductase activity. Our results show a correlation between decreased fork-displacement rates and alterations in ribonucleotide reductase, suggesting a possible involvement of this enzyme in the replication apparatus.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000201:enzymology / Q000235:genetics*; D051379:Mice; D009154:Mutation; D012264:Ribonucleotide Reductases / Q000235:genetics*
D004273:DNA, Neoplasm; D012264:Ribonucleotide Reductases