Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.
A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.
D000595:Amino Acid Sequence; D000818:Animals; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007304:Insect Viruses / Q000235:genetics*; D007313:Insecta / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
D006023:Glycoproteins; D007376:Interleukin-2; D011994:Recombinant Proteins