The specific inhibition of 11-cis-retinyl palmitate formation in the frog eye by diaminophenoxypentane, an inhibitor of rhodopsin regeneration.
The antischistosomal drug 1,5-di-(p-aminophenoxy) pentane (DAPP), an inhibitor of rhodopsin regeneration in the vertebrate retina, is shown to completely block the production of 11-cis-retinyl palmitate in the frog eye. An untreated frog generates a large amount of 11-cis-retinyl palmitate during 1-2 days in the dark after a strong bleach. Also, it is demonstrated that DAPP can deplete the stores of 11-cis-retinyl palmitate in the dark-adapted frog eye. The specificity of DAPP's inhibition of dark-adaptation is explored, and the usefulness of employing retinotoxic drugs to investigate the physiology and biochemistry of rhodopsin regeneration is discussed.
D000814:Aniline Compounds / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003623:Dark Adaptation / Q000187:drug effects; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D011894:Rana pipiens; D012160:Retina / Q000378:metabolism*; D000084562:Retinyl Esters; D012243:Rhodopsin / Q000096:biosynthesis; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000814:Aniline Compounds; D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate; C000900:1,5-bis(p-aminophenoxy)pentane; D012243:Rhodopsin