title: Amphibian lymphokines: 1. Leucocyte chemotactic factors produced by amphibian spleen cells following antigenic and mitogenic challenge in vitro.
abstract: The production of soluble chemotactic factors by Rana temporaria leucocytes following in vitro stimulation with antigen or mitogen was examined. Stimulated spleen cells were found to produce a soluble factor with a molecular weight (MW) of between 16,000-27,000 daltons which was chemotactic for Rana peritoneal cells. Rana peritoneal cells also showed directional movement towards solutions of casein, which is a potent chemo-attractant for mammalian lymphoid cells.
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001202:Ascitic Fluid / Q000276:immunology; D002364:Caseins / Q000276:immunology; D002630:Chemotactic Factors / Q000096:biosynthesis*; D003208:Concanavalin A / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D066298:In Vitro Techniques; D008222:Lymphokines / Q000096:biosynthesis*; D008970:Molecular Weight; D011896:Rana temporaria / Q000276:immunology*; D013154:Spleen / Q000276:immunology*
chemical_list: D000941:Antigens; D002364:Caseins; D002630:Chemotactic Factors; D008222:Lymphokines; D003208:Concanavalin A