title: A rapid HPLC technique for determining levels of histamine in tears from normal and inflamed human eyes.
abstract: Because of the important role of histamine in the inflammatory process, the measurement of histamine levels in tears has been considered as a possible index of eye irritancy. Histamine levels have been measured using an HPLC procedure involving fluorimetric detection after fluorescamine derivatization. Analyses, at picomole levels, were carried out in less than 5 minutes using 10 microliter of tear samples collected from either normal or inflamed human eyes.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D009877:Endophthalmitis / Q000378:metabolism*; D006632:Histamine / Q000032:analysis*; D006801:Humans; D007509:Irritants / Q000633:toxicity; D013666:Tears / Q000032:analysis*
chemical_list: D007509:Irritants; D006632:Histamine