Implication of proteases in the respiration dependent inactivation of the lactose permease of E. coli.
The lactose permease of E. coli becomes irreversibly inactivated during lactose transport under conditions of high respiratory activity. This inactivation is characterized by a decrease in the steady state of lactose accumulation, a decrease in the influx rate of lactose, and a decrease in the transmembrane electrical potential. We report here that inhibitors of serine proteases (phenylmethylsulfonyl fluoride and N-alpha-P-tosyl-L-lysine chloromethyl ketone) prevent this inactivation, thus implicating proteases in this process.
D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D007785:Lactose / Q000378:metabolism; D008564:Membrane Potentials; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins / Q000037:antagonists & inhibitors*; D009004:Monosaccharide Transport Proteins; D010101:Oxygen Consumption; D010447:Peptide Hydrolases / Q000378:metabolism*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; D027981:Symporters; D013997:Time Factors; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology
D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D011480:Protease Inhibitors; D027981:Symporters; D014107:Tosyllysine Chloromethyl Ketone; D010664:Phenylmethylsulfonyl Fluoride; C021499:lactose permease; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; D007785:Lactose