Ability of rosetting or non-rosetting individual control and inflammatory macrophages to kill Escherichia coli X43 intracellularly.
An autoradiographic method combined with a rosette technique was used to assess the bactericidal activity of individual control and inflammatory peritoneal macrophages (PM phi) in the presence or absence of expression of Fc receptor for IgG (FcR). There was a lack of FcR reactivity in a certain percentage of both categories of PM phi exposed to E. coli X43, a bacterium which is readily phagocytosed in the presence of specific antibody. Both rosetting and non-rosetting PM phi were capable of phagocytosing E. coli X43, but inflammatory PM phi showed a marked reduction in their capacity to ingest these bacteria compared with control PM phi. Once ingested the E. coli X43 were killed equally well by non-rosetting and rosetting control and inflammatory PM phi.
D000818:Animals; D001500:BCG Vaccine / Q000494:pharmacology; D002364:Caseins / Q000494:pharmacology; D004926:Escherichia coli / Q000502:physiology*; D005260:Female; D006087:Graft vs Host Reaction; D007249:Inflammation / Q000276:immunology; D007424:Intracellular Fluid / Q000382:microbiology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010446:Peptide Fragments / Q000494:pharmacology; D010587:Phagocytosis; D011961:Receptors, Fc / Q000096:biosynthesis; D012397:Rosette Formation
D001500:BCG Vaccine; D002364:Caseins; D010446:Peptide Fragments; D011961:Receptors, Fc; C018135:proteose-peptone