High-performance liquid chromatographic method for quantitation of pentamidine in blood serum.
A high-performance liquid chromatographic procedure has been developed for the determination of pentamidine concentrations in serum samples. A microbore, reversed-phase column was used with a mobile phase consisting of methanol and water with sodium heptanesulfonate and triethylamine as modifiers. Pentamidine could be extracted from serum only by the addition of an ion-pairing agent, di(2-ethylhexyl) phosphoric acid, to the chloroform used for extraction. The method can be used to reliably detect levels as low as 5 ng/ml. The pentamidine concentration in the serum of eleven patients 24 h after their tenth daily dose of pentamidine averaged 60 +/- 34 ng/ml.
D000578:Amidines / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D010419:Pentamidine / Q000097:blood* / Q000627:therapeutic use; D011020:Pneumonia, Pneumocystis / Q000188:drug therapy
D000578:Amidines; D010419:Pentamidine