title: Influence of different inhibitors on the activity of the RAD54 dependent step of DNA repair in Saccharomyces cerevisiae.
abstract: The recombinagenic pathway of DNA repair in yeast was characterized by the effect of different inhibitors on the temperature-dependent survival after gamma-irradiation in haploid cells of the thermoconditional mutant rad54-3. Blocking protein synthesis with cycloheximide in replicating cells caused partial inhibition of the RAD54 dependent function but some repair activity remained detectable. This indicates that gamma-rays can induce RAD54 activity above some constitutive level of function. Inhibition of DNA replication by hydroxyurea efficiently blocked the RAD54 dependent function in stationary-phase cells but not in logarithmic-phase cells. In logarithmic-phase cells, we found a strong inhibitory effect of caffeine on the RAD54 mediated repair process.
mesh_terms: D002110:Caffeine / Q000494:pharmacology*; D002470:Cell Survival / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D003513:Cycloheximide / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004271:DNA, Fungal / Q000528:radiation effects; D005720:Gamma Rays; D006358:Hot Temperature; D006918:Hydroxyurea / Q000494:pharmacology*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D003037:Cobalt Radioisotopes; D004271:DNA, Fungal; D002110:Caffeine; D003513:Cycloheximide; D006918:Hydroxyurea