In vivo clearance and tissue uptake of an anti-DNA monoclonal antibody and its complexes with DNA.
In vivo clearance and tissue localization of a purified mouse anti-DNA monoclonal antibody (MoAb) (A52 IgG2b) and its complexes with DNA were studied in normal BALB/c and autoimmune NZB/NZW mice. The plasma half-life of the autoantibody in both mouse strains was significantly shorter (T 1/2 = 10-15 min), compared with that of purified NZB myeloma proteins (T 1/2 greater than or equal to 180 min). DNA antigen and DNA-A52 IgG complexes in antibody excess were cleared very rapidly (T 1/2 = 4-8 min), while complexes formed in antigen excess persisted in the circulation much longer (T 1/2 = 60 min). Organ studies showed that the anti-DNA MoAb was transiently retained by the liver and the spleen but demonstrated a particular affinity for the kidney tissue. We suggest that tissue damage in SLE glomerulonephritis may be facilitated by direct interaction of anti-DNA antibodies with glomerular components.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D004247:DNA / Q000276:immunology* / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006207:Half-Life; D007074:Immunoglobulin G / Q000276:immunology; D007668:Kidney / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D014018:Tissue Distribution
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D004247:DNA