Enzyme immunoassay for detection of Chlamydia trachomatis from the cervix.
An enzyme immunoassay for the detection of Chlamydia trachomatis antigens was compared with isolation of the organism in cycloheximide-treated McCoy cells using duplicate cervical swab specimens from 514 women. Chlamydia trachomatis was isolated after subculturing from 45 of 514 (8.8%) specimens, while the enzyme immunoassay, Chlamydiazyme detected chlamydial antigens in 37 of 45 (82.2%) of those women. Chlamydiazyme was as sensitive as primary isolation for detecting total infections. The specificity of Chlamydiazyme was 98.1%. The predictive values of a positive and negative Chlamydiazyme test compared with isolation after subculturing was 80.4 and 98.3%, respectively. Overall, 497 of 514 (96.7%) samples were identified correctly by Chlamydiazyme. Thus, Chlamydiazyme is a simple and rapid test that eliminates many of the problems encountered by isolation procedures.
D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis*; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000276:immunology / Q000302:isolation & purification; D002986:Clinical Trials as Topic; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D002575:Uterine Cervicitis / Q000175:diagnosis* / Q000382:microbiology
D000942:Antigens, Bacterial