Inhibitor of 2',5'-oligoadenylate synthetase induced in human T lymphoblastoid cell line treated with deoxyadenosine, deoxycoformycin and interferon.
The effect of deoxyadenosine (dAdo) with deoxycoformycin on the induction of 2',5'-oligoadenylate synthetase by interferon was investigated. After semi-purification through poly(I):poly(C) gel, the activity was similar in control and dAdo-treated cells. However, the activity in the crude extract decreased with rising concentrations of dAdo. On the other hand, the level of 2'-phosphodiesterase, which is also induced by interferon and degrades 2',5'-oligoadenylate, showed no significant change after dAdo treatment. Thus, the crude extract was speculated to contain an inhibitor of 2',5'-oligoadenylate synthetase. Further characterization of the inhibitor revealed that inhibition was not due to dATP accumulation in cells.
D015088:2',5'-Oligoadenylate Synthetase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000243:Adenosine Deaminase / Q000172:deficiency; D002460:Cell Line; D003070:Coformycin / Q000031:analogs & derivatives / Q000494:pharmacology*; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D003839:Deoxyadenosines / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005095:Exoribonucleases / Q000378:metabolism; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000201:enzymology; D007370:Interferon Type I / Q000494:pharmacology*; D015649:Pentostatin; D012263:Ribonucleosides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*
D003838:Deoxyadenine Nucleotides; D003839:Deoxyadenosines; D007370:Interferon Type I; D012263:Ribonucleosides; D003070:Coformycin; D015649:Pentostatin; D015088:2',5'-Oligoadenylate Synthetase; D005095:Exoribonucleases; C029364:2'-phosphodiesterase; D000243:Adenosine Deaminase; C026600:2'-deoxyadenosine triphosphate