Biologic activities of recombinant human interleukin 2 on murine lymphocytes.
Recombinant human IL-2, produced by yeast cells, was tested in a number of in vitro responses with murine lymphocytes. The responses studied included proliferation of a cloned murine T lymphocyte line, generation of cytotoxic responses, recall of cytotoxic memory, and restoration of responses in spleen cells taken from cyclophosphamide-treated mice. In all cases, the recombinant human IL-2 had the same activity as purified murine IL-2. The recombinant material represents a source of IL-2 free of other lymphokines. The responses described in this work can therefore be ascribed to the direct effects of human IL-2, of known sequence, on the cells of interest.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003520:Cyclophosphamide / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007156:Immunologic Memory / Q000187:drug effects; D007376:Interleukin-2 / Q000096:biosynthesis / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects*
D007376:Interleukin-2; D003520:Cyclophosphamide