Cloning and expression of the human erythropoietin gene.
The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004921:Erythropoietin / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization
D004921:Erythropoietin