title: Glycosaminoglycans in human gingival crevicular fluid as indicators of active periodontal disease.
abstract: The glycosaminoglycans (GAG) in gingival crevicular fluid (GCF) were investigated by cellulose-acetate electrophoresis of samples from individual sites of defined conditions variously affecting the tissues of the periodontium. The non-sulphated GAG, hyaluronic acid, was present in all samples and was the only major band from sites of chronic gingivitis. An additional sulphated GAG band identified by enzymic digestions as chondroitin-4-sulphate, was detected in GCF from sites of untreated-advanced periodontitis. Initial samples from sites of early periodontitis and juvenile periodontitis yielded a similar additional band which was not detected, however, in samples collected after either surgery to eliminate deep pockets or daily subgingival irrigation with a chlorhexidine solution. Sulphated GAG was also present in fluid from the control situations, i.e. of teeth either undergoing orthodontic movement or showing evidence of trauma from occlusion, and from healing tooth-extraction wounds. Thus the presence of such a component in GCF correlates with those clinical conditions in which degradative changes are occurring in the deeper-periodontal tissues. The electrophoretic profile of GAG in a sample of GCF may be a sensitive laboratory method of indicating active phases of destructive periodontal disease at individual sites.
mesh_terms: D002908:Chronic Disease; D004588:Electrophoresis, Cellulose Acetate; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D005891:Gingivitis / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D010510:Periodontal Diseases / Q000175:diagnosis* / Q000628:therapy; D010518:Periodontitis / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans