Cell-surface modification with an iodinatible imidoester to enhance radiolabeling.
A procedure to enhance incorporation of radioactive iodine into cell-surface proteins is described. The effects of chemical modification of cells with the iodinatible haptens N-succinimidyl-3-(4-hydroxyphenyl) propionate (Bolton-Hunter reagent) and methyl-p-hydroxybenzimidate HC1 (Wood reagent) are compared. The Wood reagent, that does not alter the charge of the modified amino group, is superior in retention of cell viability and function. Cell-surface modification with the Wood reagent prior to radiolabeling is particularly useful for detecting antigens not readily iodinated using standard techniques.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D002470:Cell Survival; D007096:Imidoesters; D007457:Iodine Radioisotopes; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D013388:Succinimides; D013601:T-Lymphocytes / Q000166:cytology
D007096:Imidoesters; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D013388:Succinimides; C022581:Bolton-Hunter reagent; C014099:methylhydroxybenzimidate