Abnormal amino acid metabolism in mutants of Saccharomyces cerevisiae affected in the initiation of sporulation.
Amino acid metabolism was examined during sporulation in a wild-type strain of Saccharomyces cerevisiae and in a mutant homozygous for the spd1 mutation (derepression of sporulation). In the wild-type initiation of sporulation involves a rise in intracellular glutamate. In the derepressed mutant there was no further increase of intracellular glutamate: glutamate was already present at a greater concentration than in the wild-type. These elevated glutamate levels are apparently due to reduction in the activity of 2-oxoglutarate dehydrogenase. One consequence of the elevated glutamate levels in diploids homozygous for the spd1 mutation is the production of considerable amounts of glycyl-L-proline, glutathione and saccharopine.
D000596:Amino Acids / Q000302:isolation & purification / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D009154:Mutation; D010455:Peptides / Q000302:isolation & purification; D011392:Proline / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal / Q000378:metabolism*
D000596:Amino Acids; D005971:Glutamates; D010455:Peptides; D011392:Proline; D007655:Ketoglutarate Dehydrogenase Complex