Impaired organic ion transport in proximal tubules of rats with Heymann nephritis.
Organic ion transport across the basolateral membrane of proximal tubules was measured by means of the tissue slice technique in each of the four different stages of Heymann nephritis. Impairment of both organic anion and cation transport was detected early in Stage 2, and became more severe in Stage 3 of Heymann nephritis. The decreased transport function was associated with extensive damage to proximal tubule cells, including loss of brush border microvilli and basal infoldings. Despite these abnormalities of structure and function, oxygen consumption of proximal tubule cells remained essentially normal. Partial recovery of organic cation transport was noted late in Heymann nephritis (Stage 4). Recovery of the cation transport function was associated with a partial restoration of brush border microvilli and basal infoldings to proximal tubule cells. However, organic anion transport remained depressed throughout the entire course of disease. Impairment of organic ion transport in rats with Heymann nephritis appeared to result from damage to basolateral membrane transport elements rather than general deterioration of the metabolic machinery of proximal tubule cells. Decreased organic cation transport appeared to be the consequence of a reduction in the number of carrier sites, a phenomenon that could have resulted from decreased membrane surface area. However, the depression of organic anion transport was associated with decreased substrate affinity of the anion carrier, indicating that qualitative, rather than quantitative changes, were primarily responsible for that defect. Specific antibody-mediated damage to the anion transport elements in basolateral membranes of proximal tubules is postulated to occur in Heymann nephritis.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D001327:Autoimmune Diseases / Q000503:physiopathology*; D001693:Biological Transport, Active; D005260:Female; D007477:Ions; D007687:Kidney Tubules, Proximal / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009161:Mycobacterium; D009393:Nephritis / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011917:Rats, Inbred Lew; D010130:p-Aminohippuric Acid / Q000378:metabolism
D000276:Adjuvants, Immunologic; D007477:Ions; D010130:p-Aminohippuric Acid