Regulation of human granulocyte function by products derived from murine tumors.
Murine EL-4 thymoma was found to produce a factor that activated antibody-dependent cell-mediated cytotoxicity by human eosinophils and neutrophils. The eosinophil-activating factor had some properties similar to human eosinophil colony-stimulating factor in being heat stable, inactivated by 2-mercaptoethanol, and cochromatographing by gel filtration with a factor that promoted the growth of pure eosinophil colonies from human bone marrow. The neutrophil-activating factor from EL-4 was also heat stable but was not inactivated by 2-mercaptoethanol and was found in two peaks on chromatography, neither having human neutrophil-CSF activity but one cochromatographing with mouse granulocyte-macrophage CSF. EL-4 therefore secretes factors that activate two types of human granulocytes. Several other murine thymomas and macrophage cell lines produced a neutrophil-activating factor, the production of which could not be correlated with that of several other known lymphokines. Murine tumors produce factors that activate human granulocytes.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D001402:B-Lymphocytes / Q000032:analysis; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D004804:Eosinophils / Q000502:physiology*; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D006825:Hybridomas / Q000032:analysis; D008264:Macrophages / Q000032:analysis; D051379:Mice; D009504:Neutrophils / Q000502:physiology*; D013045:Species Specificity; D013945:Thymoma / Q000378:metabolism*; D013953:Thymus Neoplasms / Q000378:metabolism*
D003115:Colony-Stimulating Factors