Mutagenicity of beta-glucuronidase in the Ames test.
The enzyme preparation beta-glucuronidase/arylsulphatase from Helix pomatia (Boehringer) caused base-pair substitutions in Salmonella typhimurium TA100 and TA1535 strains within the dose range of 0.50-50 microliter per plate. No effect was observed in the TA98 strain. The presence of S9 mix did not substantially affect the mutagenic potential of beta-G. The number of induced revertants decreased continually from experiment to experiment carried out in the course of 12 weeks.
D000818:Animals; D001711:Biotransformation; D005966:Glucuronidase / Q000378:metabolism / Q000633:toxicity*; D006372:Helix, Snails / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics*
D009153:Mutagens; D005966:Glucuronidase