Location of the SH group of the alkali light chain on the myosin head as revealed by electron microscopy.
The location of the single cysteinyl residue of the alkali light chain on the myosin head was determined by electron microscopy. The cysteinyl residue of isolated alkali light chain 2 was biotinylated and the light chain was exchanged with that of heavy meromyosin in 4.7 M-NH4Cl. Avidin was attached to the biotin in the heavy meromyosin and the complex was rotary shadowed and observed in the electron microscope. The distance from the head-rod junction to the centre of avidin was 8(+/- 3) nm (mean value +/- standard deviation: n = 105).
D000818:Animals; D001360:Avidin; D001665:Binding Sites; D003545:Cysteine; D008854:Microscopy, Electron; D015879:Myosin Subfragments; D009218:Myosins; D011817:Rabbits
D015879:Myosin Subfragments; D001360:Avidin; D009218:Myosins; D003545:Cysteine