Spontaneous production of antibodies to deoxyribonucleic acids in patients with systemic lupus erythematosus.
In vitro spontaneous anti-DNA antibody production in patients with systemic lupus erythematosus (SLE) was examined. SLE lymphocytes produced IgG and IgM anti-DNA antibody from the third culture day, and reached a plateau on the seventh culture day. This anti-DNA antibody activity in 7-day culture supernatant was abolished by pretreatment of the lymphocytes with cycloheximide, suggesting de novo immunoglobulin synthesis was required for this spontaneous anti-DNA antibody production. Lymphocytes from patients with rheumatoid arthritis (RA) and other collagen diseases including progressive systemic sclerosis, polymyositis/dermatomyositis, and polyarteritis nodosa did not produce IgG and IgM anti-DNA antibody spontaneously, but SLE lymphocytes produced substantial amounts of IgG and IgM anti-DNA antibody spontaneously. Furthermore, active SLE produced a larger amount of IgG anti-DNA antibody than inactive SLE. We observed a significant negative correlation between the number of Ia+ T cells and IgG, but not IgM, anti-DNA antibody production. Furthermore, spontaneous IgG anti-DNA antibody production was elevated after pretreatment of SLE T cells with anti-Ia and complement, suggesting that Ia+ T cells in SLE bring about suppression of autologous B cells producing IgG anti-DNA antibody.
D000293:Adolescent; D000328:Adult; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000378:metabolism; D001323:Autoantibodies / Q000276:immunology; D002648:Child; D003095:Collagen Diseases / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000187:drug effects; D013997:Time Factors
D001323:Autoantibodies; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins; D004247:DNA; D003513:Cycloheximide