The effect of tunicamycin on transferrin synthesis and secretion in hormonally stimulated explants of rabbit mammary gland.
Pregnant rabbit mammary gland explants cultured with insulin, prolactin and cortisol synthesize and secrete transferrin radiolabelled with [3H]leucine or [3H]mannose. Addition of tunicamycin inhibits the glycosylation but not synthesis of the [3H]leucine-labelled polypeptide. Secretion of the non-glycosylated protein is only marginally inhibited.
D000818:Animals; D005260:Female; D005944:Glucosamine / Q000031:analogs & derivatives*; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011817:Rabbits; D014168:Transferrin / Q000096:biosynthesis* / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology*
D007328:Insulin; D014168:Transferrin; D014415:Tunicamycin; D011388:Prolactin; D005944:Glucosamine; D006854:Hydrocortisone