Flow cytometry of ethanol-fixed versus fresh bladder barbotage specimens.
Saline bladder barbotage specimens were obtained from 99 patients for comparison of flow cytometric deoxyribonucleic acid analyses using fresh versus ethanol-fixed cell preparation techniques. The analyses were consistent for the 2 methods in 87 (88 per cent) of the 99 cases (p less than 0.0001, Kappa statistic for agreement). Since accurate, reproducible results are obtainable on fixed bladder washings, samples may be sent to flow cytometry centers for analysis.
D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D000431:Ethanol; D005404:Fixatives; D005434:Flow Cytometry; D006652:Histological Techniques; D006801:Humans; D013048:Specimen Handling / Q000379:methods; D007507:Therapeutic Irrigation; D001743:Urinary Bladder / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology
D004273:DNA, Neoplasm; D005404:Fixatives; D000431:Ethanol; D004247:DNA