The regulation of tissue eosinophilia. III. In vitro production of eosinophil-directed chemotactic inhibitory factor by T lymphocytes of complete Freund's adjuvant-treated guinea-pigs.
Guinea-pig spleen cells treated with complete Freund's adjuvant (CFA) produce eosinophil-directed chemotactic inhibitory factors (ECIF). The inhibition is selective for the response of eosinophils to delayed ECF-a, which had been isolated from 24-hr-old inflamed skin lesions induced by DNP-Ascaris extract in sensitized animals and had been confirmed as being a T lymphocyte-derived lymphokine. ECIF activity is absorbed by incubation with eosinophils, but not with macrophages or neutrophils. The cells spontaneously release ECIF; pretreatment with a protein synthesis inhibitor reduces ECIF production, indicating that protein synthesis is essential. The source of ECIF seems to be lymphocytes, probably T lymphocytes. ECIF activity is recovered in two separate fractions: one elutes near bovine serum albumin (MW 70,000) and the other near cytochrome c (MW 12,500). ECIF binds to peanut agglutinin- or Limulus polyphemus agglutinin-coupled agarose beads. Furthermore, ECIF activity is blocked when eosinophils are incubated with D-galactose or sialic acid. These results suggest that ECIF derived from T lymphocytes of CFA-treated animals modulate the delayed ECF-a-mediated tissue eosinophilia, and that terminal galactose and/or sialic acid residues are essential for ECIF activity.
D000818:Animals; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors*; D002631:Chemotactic Factors, Eosinophil / Q000037:antagonists & inhibitors*; D002634:Chemotaxis, Leukocyte; D004802:Eosinophilia / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006168:Guinea Pigs; D007700:Kinetics; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D009005:Monosaccharides / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil; D008222:Lymphokines; D009005:Monosaccharides; C034397:chemotactic inhibitory factor; D005620:Freund's Adjuvant