Expansion and cell surface Ig isotype switching in the antigen-binding cell population of nu/nu mice and nu/+ littermates.
Swiss-Webster nu/nu splenocytes placed in modified Mishell-Dutton culture containing sheep red blood cells (SRC) generated increased numbers of antigen-binding cells (ABC) compared with antigen-free cultures. In contrast Balb/c nu/nu cultures did not expand their ABC population in response to SRC, suggesting that strain background may influence the effect of the nu/nu gene on T-dependent immune responses. Cell surface Ig isotype analysis indicated that the SRC-induced expanded ABC population exhibited a significant decrease in cell surface IgD and a significant increase in ABC bearing both IgM and IgG. The Swiss-Webster nu/+ littermate cell surface Ig isotype patterns were generally similar to the nu/nu ABC patterns, but with different kinetics.
D000818:Animals; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007114:Immunization; D007072:Immunoglobulin D / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell