Expression of Ia antigens on the surface of human oligodendrocytes and astrocytes in culture.
Oligodendrocytes and astrocytes were isolated from normal adult human brains 3-15 h postmortem using a Percoll density gradient centrifugation and were cultured for 10-135 days. The presence of HLA-DR(Ia) antigens on the surface of these human oligodendrocytes and astrocytes was studied using double immunofluorescence procedures. Only half of separate culture series (6/12 donors) contained HLA-DR-positive oligodendrocytes, while all of the culture series (12/12 donors) revealed HLA-DR-positive astrocytes. Among the HLA-DR-positive cultures, 4-16% of galactocerebroside-positive oligodendrocytes and 9-24% of GFAP-positive astrocytes were found to immunoreact with HLA-DR antibody. The presence of Ia antigens on the surface of certain populations of oligodendrocytes and astrocytes may be important in the induction of an immune response to these cells.
D000328:Adult; D000368:Aged; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001253:Astrocytes / Q000276:immunology*; D001921:Brain / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009457:Neuroglia / Q000276:immunology*; D009836:Oligodendroglia / Q000276:immunology*
D000954:Antigens, Surface; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II