[Detection of hormonally dependent albumin secretion by primary cultures of rat hepatocytes using an homologous system of radioimmunological analysis].
A homologous radioimmunoassay to determine rat serum albumin is described in detail. Using this technique one was able to estimate albumin basal secretion by initial rat hepatocyte cultures in a serum-free medium and to detect protein secretion changes caused by various hormones. The stimulating dose-dependent affects of insulin and growth hormone as well as the inhibitory effect of estradiol with regard to albumin production by cultivated hepatocytes were demonstrated. The time of incubation with hepatic cells which was necessary to reach a maximum expression of the effect, was different for various hormones. The preliminary results provide an opportunity to create models for in vitro studies on various dyshormonal states of the body on the basis of a quantitative estimation of hormone-dependent albumin secretion by initial hepatocyte cultures.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology; D007114:Immunization; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D012711:Serum Albumin, Radio-Iodinated
D000906:Antibodies; D007328:Insulin; D012709:Serum Albumin; D012711:Serum Albumin, Radio-Iodinated; D004958:Estradiol; D013006:Growth Hormone
Vyiavlenie gormonal'no-zavisimoĭ sekretsii al'bumina pervichnymi kul'turami gepatotsitov krys s pomoshch'iu gomologichnoĭ sistemy radioimmunologicheskogo analiza.