[Retrospective electrophoretic analysis of the individual precipitates from the dried and stained immunoelectrophoregrams of crystalline lens proteins].
Electrophoretic analysis of pattern of polypeptides making part of precipitation arcs (peaks) cut out of predried and stained immunoelectrophoregrams has been shown to be possible. The cut out precipitation arcs were extracted overnight by a starting buffer for SDS-electrophoresis, centrifuged, and supernatants were subjected to SDS-electrophoresis; electrophoregrams were stained by silver nitrate. Electrophoretic pattern of polypeptides from the stained and "native" precipitation arcs for the same antigens were shown to be identical. A good correspondence was also observed between the results of analysis of polypeptide composition of the immune precipitates and the data of immunoelectroblotting preformed with the same immune reagents. This technique allows precipitation arcs to be studied from immunoelectrophoregrams preserved at the room temperature for a decade and more.
D000818:Animals; D003459:Crystallins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007122:Immunoelectrophoresis / Q000379:methods*; D007525:Isoelectric Focusing; D010455:Peptides / Q000032:analysis; D010999:Pleurodeles; D011233:Precipitin Tests / Q000379:methods; D011817:Rabbits; D011895:Rana ridibunda; D011896:Rana temporaria; D012189:Retrospective Studies
D003459:Crystallins; D010455:Peptides
Retrospektivnyĭ élektroforeticheskiĭ analiz individual'nykh pretsipitatov iz vysushennykh i okrashennykh immunoélektroforegramm belkov khrustalika glaza.