Measurement of pancreatic and salivary alpha-amylase in serum: comparison of methods with five different substrates.
Pancreatic and salivary alpha-amylase in human serum have been determined with the inhibitor method with 5 different substrates. An end concentration of 60 inhibitor units per liter reagent is sufficient for inhibition of the salivary alpha-amylase. The recovery of known amounts of pancreatic and salivary alpha-amylase was excellent. A mean +/- SD of 53.1 +/- 3.8% of pancreatic alpha-amylase as percentage of total alpha-amylase in serum has been observed for the 5 different substrates used. Moreover, it seems that standards obtained from crude saliva or duodenal fluid can be used as well as pure enzymes obtained from a commercial supplier.
D005960:Glucosides; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000037:antagonists & inhibitors / Q000097:blood*; D008320:Maltose / Q000031:analogs & derivatives; D009844:Oligosaccharides; D010179:Pancreas / Q000201:enzymology*; D012015:Reference Standards; D012463:Saliva / Q000201:enzymology*; D013213:Starch; D013379:Substrate Specificity; D000516:alpha-Amylases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000097:blood*
D005960:Glucosides; D007527:Isoenzymes; D009844:Oligosaccharides; D008320:Maltose; C036167:4-nitrophenyl maltoheptaoside; D013213:Starch; C009819:maltotetraose; D000516:alpha-Amylases