title: Langerhans cells and extra-epidermal dendritic cells. An investigation in laboratory animals and man with immunomorphological methods.
abstract: S-100 protein was demonstrated in the cytoplasm of dendritic cells (DCs) in normal and pathologic lymphoid tissues and epidermis in man and several other species. The presence of S-100 protein served to distinguish these cells from other mononuclear cells, most importantly from those of macrophage/histiocyte lineage. Fractionation procedures to isolate and enrich suspensions of DCs were coupled with immunocytochemical techniques to identify S-100-positive cells. Langerhans cells in the epidermis and in aural cholesteatomata and nodal, splenic, and thymic interdigitating cells were S-100-positive. Lymph node and splenic follicular dendritic cells (except in rats) were negative, indicating that this DC may be a separate cell type.
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D002356:Cartilage / Q000166:cytology; D002417:Cattle; D002469:Cell Separation; D002781:Cholesteatoma / Q000378:metabolism; D006168:Guinea Pigs; D006646:Histiocytosis, Langerhans-Cell / Q000378:metabolism; D006801:Humans; D007120:Immunochemistry; D007801:Langerhans Cells / Q000032:analysis*; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000032:analysis / Q000166:cytology*; D051379:Mice; D008854:Microscopy, Electron; D051381:Rats; D009418:S100 Proteins / Q000032:analysis* / Q000276:immunology; D012867:Skin / Q000166:cytology; D013154:Spleen / Q000166:cytology
chemical_list: D009418:S100 Proteins