Effect of islet cell surface antibodies on neonatal rat pancreatic islet cells or isolated islets of Langerhans in vitro.
Islet cell surface antibodies (ICSA) raised by immunizing rabbits with pancreatic islet cell suspensions were characterized with respect to some aspects of their influence on neonatal rat islet cells or isolated islets of Langerhans. The removal of unspecific cytotoxic factors by absorption with liver powder and spleen cells was reflected in changes of the antibody binding pattern to islet cells demonstrated by flow-cytometric analysis as well as the corresponding 51Cr-release from prelabelled islet cells. Using neonatal rat pancreatic islets as a target, fresh ICSA-positive serum provokes a beta-cell specific insulin leakage in a concentration dependent manner. In contrast to heat-inactivated antiserum the complement-mediated cytotoxic effect of rabbit anti-rat islet cell surface antiserum seems to have its morphological expression also in alterations of the islet surface structure as revealed by scanning electron microscopy.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000954:Antigens, Surface / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D002478:Cells, Cultured; D007515:Islets of Langerhans / Q000276:immunology* / Q000648:ultrastructure; D051381:Rats
D000954:Antigens, Surface; D001323:Autoantibodies