A new form of amyloid protein associated with chronic hemodialysis was identified as beta 2-microglobulin.
Amyloid fibrils were isolated from amyloid-laden tissue obtained from a chronic hemodialysis patient with carpal tunnel syndrome. After solubilization in guanidine HCl, a significant amount of the protein was located in a homogeneous low molecular weight fraction. The protein was found to be identical to beta 2-microglobulin, with regard to its molecular weight of 11,000, amino acid composition and 16 amino-terminal amino acids: Ile-Gln-Arg-Thr-Pro-Lys-Ile-Gln-Val-Tyr-Ser-Arg-His-Pro-Ala-Glu-. These results demonstrate that the amyloid associated with chronic hemodialysis contains as major component a new form of amyloid fibril protein that is homologous to beta 2-microglobulin.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000686:Amyloidosis / Q000209:etiology*; D002349:Carpal Tunnel Syndrome / Q000209:etiology / Q000601:surgery; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005921:Glomerulonephritis / Q000150:complications; D006801:Humans; D007676:Kidney Failure, Chronic / Q000209:etiology / Q000628:therapy; D008875:Middle Aged; D008970:Molecular Weight; D006435:Renal Dialysis / Q000009:adverse effects*; D001613:beta 2-Microglobulin / Q000032:analysis*
D000596:Amino Acids; D001613:beta 2-Microglobulin