Characterization and localization of HLA antigens on hydatidiform mole.
Frozen sections of three specimens of hydatidiform mole were stained with monoclonal antibodies to HLA Class I and Class II antigens by means of an indirect immunoperoxidase technique. Class I (HLA A, B, C) antigens were detected on proliferating extravillous trophoblast and on villous stromal cells but not on quiescent villous trophoblast. Trophoblast Class I antigen was detected with four different antibodies to monomorphic determinants but not with antibodies to the appropriate polymorphic HLA A or B type. Stromal cells were reactive with all Class I antibodies. Class II (HLA DR) antigens were not detected on any molar tissue. The expression of HLA antigens by molar tissue is similar to that of the normal first-trimester human placenta.
D000911:Antibodies, Monoclonal / Q000276:immunology; D005260:Female; D006680:HLA Antigens / Q000302:isolation & purification*; D006801:Humans; D006828:Hydatidiform Mole / Q000276:immunology / Q000473:pathology*; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D014327:Trophoblasts / Q000276:immunology / Q000473:pathology; D014594:Uterine Neoplasms / Q000276:immunology / Q000473:pathology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens