Phosphorylation of 3-O-methyl-D-glucose and catabolite repression in yeast.
The glucose analog, 3-O-methyl-D-glucose, inhibited growth of yeast on non-fermentable carbon sources. The sugar was phosphorylated by the yeast and also in vitro by a commercial preparation of yeast hexokinase. The chromatographic behaviour of the phosphorylated product was identical in both cases. This suggests that 3-O-methyl-D-glucose is phosphorylated to form 3-O-methyl-D-glucose 6-phosphate. The inhibition of the growth appears to be due to interference with the derepression of several enzymes necessary to grow on non-fermentable carbon sources. Spontaneous mutants whose growth was unaffected by 3-O-methyl-D-glucose were isolated. In these mutants there was no significant accumulation of the phosphorylated ester and the derepression of the enzymes tested was not affected by the glucose analog.
D019325:3-O-Methylglucose; D002854:Chromatography, Paper; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D010766:Phosphorylation; D010940:Plant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D008757:Methylglucosides; D008759:Methylglycosides; D010940:Plant Proteins; D019325:3-O-Methylglucose