Dominant and recessive mitogen-nonproliferative variants of 3T3 cells.
We have previously isolated 3T3 cell variants unable to respond to specific mitogens. In this report we analyze the dominant and/or recessive nature of these variants. Two independently isolated EGF nonproliferative variants are unable to bind EGF. Hybrids between 3T3R5 cells (thymidine kinase deficient, ouabain-resistant) and these variants express EGF receptors; the "EGF receptorless" phenotype of these variants is recessive. Hybrids between these two variants do not bind EGF; they are defective in a common, non-complementing function. A TPA nonproliferative 3T3 variant is also recessive; hybrids with 3T3R5 mount a mitogenic response to TPA. In contrast a fourth variant, which can neither bind labeled EGF nor respond to TPA, is dominant for both characteristics. Hybrids between this latter variant and 3T3R5 can neither bind EGF nor mount a mitogenic response to TPA.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002468:Cell Physiological Phenomena; D004815:Epidermal Growth Factor / Q000235:genetics; D005799:Genes, Dominant; D005808:Genes, Recessive; D014644:Genetic Variation; D051379:Mice / Q000196:embryology; D008934:Mitogens / Q000235:genetics* / Q000494:pharmacology; D010641:Phenotype; D013755:Tetradecanoylphorbol Acetate / Q000235:genetics
D008934:Mitogens; D004815:Epidermal Growth Factor; D013755:Tetradecanoylphorbol Acetate