title: Presence of tropomyosin in adrenal chromaffin cells and its association with chromaffin granule membranes.
abstract: The presence of tropomyosins in adrenal chromaffin cells was demonstrated by immunoperoxidase staining of cultured chromaffin cells by an anti-tropomyosin antibody. In immunoblotting the antibody labelled polypeptides of Mr 39 000, 36 500 and 31 000 in 100 000 X g supernatants and heat-stable fractions of adrenal medulla. The adrenal medullary tropomyosins did not comigrate with smooth muscle tropomyosins. The 39-kDa and 31-kDa polypeptides comigrated with polypeptides labelled by anti-tropomyosin in brain supernatants. Chromaffin granule membranes possessed a 39-kDa polypeptide that was labelled by anti-tropomyosin.
mesh_terms: D000313:Adrenal Medulla / Q000648:ultrastructure*; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000648:ultrastructure*; D002838:Chromaffin System / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008970:Molecular Weight; D014335:Tropomyosin / Q000032:analysis*
chemical_list: D014335:Tropomyosin