Cell-free synthesis of preuteroglobin and processing to uteroglobin by polysomes from rabbit endometrium.
Polysomes from rabbit endometrium, incubated in a cell-free system in the presence of [35S]methionine, completed polypeptide chains pre-initiated in vivo. Polypeptide chains were analyzed by SDS-gel electrophoresis after immunoprecipitation with anti-uteroglobin. Endometrial polysomes, freed of membranes by detergent treatment, synthesized both mature uteroglobin and preuteroglobin not yet cleaved in vivo. Membrane-bound polysomes synthesized exclusively mature uteroglobin, indicating the processing of preuteroglobin by the membranes bound to the ribosomes.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D004717:Endometrium / Q000648:ultrastructure*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D006493:Heparin / Q000494:pharmacology; D007163:Immunosorbent Techniques; D011092:Polyethylene Glycols / Q000494:pharmacology; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D011498:Protein Precursors / Q000096:biosynthesis*; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D014598:Uteroglobin / Q000096:biosynthesis*
D006023:Glycoproteins; D011498:Protein Precursors; D011092:Polyethylene Glycols; D006493:Heparin; D014598:Uteroglobin