title: Cloning the gene for Congo red binding in Shigella flexneri.
abstract: The ability to bind the dye Congo red from agar medium is associated with virulence of Shigella species. DNA sequences conferring this property have been cloned from a large, 140-kilobase plasmid of Shigella flexneri into a plasmid vector. This recombinant plasmid does not fully restore virulence to S. flexneri isolates which have lost the large plasmid. This indicates that other genes present on the 140-kilobase plasmid must also be required for virulence of S. flexneri. The cloned fragment contains a copy of the insertion sequence IS1 closely linked to the gene for Congo red binding.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003224:Congo Red / Q000378:metabolism*; D005798:Genes, Bacterial; D010957:Plasmids; D012763:Shigella flexneri / Q000235:genetics* / Q000472:pathogenicity; D013696:Temperature; D014774:Virulence
chemical_list: D003224:Congo Red