Ontogeny of 'macrophage' function. V. Differential effect of prostaglandin E2 on the activation of newborn and adult mouse macrophages.
Our previous papers indicate that peritoneal exudate macrophages (PM) of newborn mice are strongly suppressive for the tumor cell growth in comparison with those of adult mice. Neither newborn PM nor adult PM are cytolytic, but they both can be activated to be cytolytic by being cultured overnight either with a high concentration of bacterial lipopolysaccharide (LPS) or with a low concentration of LPS plus lymphokine. This paper shows that the activation of adult PM was inhibited by prostaglandin E2 (PGE2) added simultaneously with LPS, though that of newborn PM was not affected by PGE2. Adult PM, however, acquired the resistance to PGE2 by the preculture with LPS and/or lymphokine for 4 hr so as to be activated by LPS in the presence of PGE2. These results indicate that newborn PM are more activated than adult PM to a state which adult PM attain after moderate activation with LPS or lymphokine.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015232:Dinoprostone; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D011458:Prostaglandins E / Q000494:pharmacology*
D008070:Lipopolysaccharides; D008222:Lymphokines; D011458:Prostaglandins E; D015232:Dinoprostone