title: Immunohistochemical analysis of the early ontogeny of the neuropeptide Y system in rat brain.
abstract: The distribution of neuropeptide Y in the developing rat brain was studied with immunocytochemistry, using the peroxidase-antiperoxidase method. Immunoreactive perikarya were first seen on embryonic day 13 and staining of fibres appeared from embryonic day 15 onwards: perikaryal staining was generally more intense prenatally than after birth. Areas rich in neuropeptide Y immunostaining included the monoaminergic regions of the brain stem from embryonic day 13 (especially the lateral reticular nucleus and the medullary reticular formation), the dorsal mesencephalon (with spots of immunoreactivity in the outer subventricular zone at embryonic days 13 or 14 and many cells and fibres in the inferior colliculus from embryonic days 16-20) and the olfactory tubercle/ventral striatum from embryonic day 15 until birth. The period of development of cortical neurones extended from embryonic day 19 until postnatal day 21. A hitherto unreported feature unique to neuropeptide Y was the presence in certain parts of the cerebral cortex of transient cells at the base of the cortical plate bearing radial processes which transverse its width. They were present from embryonic day 17 until postnatal day 4 and were maximally developed at embryonic days 20 or 21, contributing at this age a substantial fibre projection through the immature corpus callosum. The abundance of neuropeptide Y in the prenatal rat brain suggests it may play an important role in development.
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D001923:Brain Chemistry; D005260:Female; D005865:Gestational Age; D007124:Immunoenzyme Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D009478:Neuropeptide Y; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y