Activation of the alternative complement pathway by pneumococcal lipoteichoic acid.
Cell wall teichoic acids of some gram-positive bacteria are potent activators of the alternative pathway of complement. It is unclear, however, whether the other form of teichoic acid, cell membrane lipoteichoic acid (LTA), can also activate the alternative pathway. In the present study, radiolabelled pneumococcal LTA was found to bind spontaneously to sheep erythrocytes in a temperature- and time-dependent fashion. In addition, the presence of pneumococcal LTA on the erythrocyte surface was verified by the fact that they could be agglutinated by a myeloma protein (TEPC-15) specific for choline, a constituent of pneumococcal LTA. Pneumococcal LTA when fixed to the surface of erythrocytes was able to activate the alternative pathway of complement in both guinea pig serum deficient in the fourth component of complement and human serum deficient in the second component of complement, resulting in lysis of the sensitized erythrocytes. The sensitizing principle of the LTA preparation was removed before erythrocyte sensitization by immunoabsorption, using the choline-specific TEPC-15 myeloma protein. These data demonstrate that purified pneumococcal LTA will bind to sheep erythrocytes and endow them with the ability to activate the alternative pathway.
D000818:Animals; D002794:Choline; D003167:Complement Activation / Q000187:drug effects*; D003170:Complement Pathway, Alternative / Q000187:drug effects*; D004912:Erythrocytes / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D007163:Immunosorbent Techniques; D008070:Lipopolysaccharides; D009194:Myeloma Proteins / Q000378:metabolism; D010712:Phosphatidic Acids / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D012756:Sheep; D013296:Streptococcus pneumoniae / Q000032:analysis*; D013682:Teichoic Acids / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
D008070:Lipopolysaccharides; D009194:Myeloma Proteins; D010712:Phosphatidic Acids; D013682:Teichoic Acids; C009900:lipoteichoic acid; D002794:Choline