Cell electrophoretic investigations of peripheral blood lymphocytes during hemodialysis with different membranes.
Determining the relative percentage of LMC in PBL histograms of RDT patients during hemodialysis we observed a temporary decrease of the LMC proportion during the first 15 minutes of hemodialysis using Cuprophan in contrast to AN 69S and MC Cellulose. These results correlate with the increase of C3a plasma concentration and the decrease of total count of granulocytes. In all investigated RDT patients higher proportions of LMC could be found in relation to healthy donors already before dialysis treatment. Our study therefore seems to indicate that the application of cell electrophoresis is a useful method for the characterization of lymphocytes during extracorporeal circulation as an additional parameter of blood compatibility.
D000180:Acrylic Resins; D000181:Acrylonitrile / Q000031:analogs & derivatives; D002482:Cellulose / Q000031:analogs & derivatives; D003176:Complement C3 / Q000378:metabolism; D015926:Complement C3a; D004586:Electrophoresis; D006801:Humans; D007688:Kidneys, Artificial / Q000009:adverse effects*; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D014511:Uremia / Q000097:blood / Q000276:immunology / Q000628:therapy
D000180:Acrylic Resins; D003176:Complement C3; C016892:AN-69; D015926:Complement C3a; D002482:Cellulose; C009865:cuprammonium cellulose; D000181:Acrylonitrile