Depressed lectin-dependent cell-mediated cytotoxicity against HEp-2 cells in patients with metastasizing solid tumour.
Lectin-dependent cell-mediated cytotoxicity (LDCC) of peripheral blood mononuclear cells (PBMC) from tumour patients was evaluated using human adherent 3H-TdR-prelabelled HEp-2 epipharynx carcinoma cells as targets at 50:1 effector-target cell ratio with 25 micrograms/ml concanavalin A (Con A) in a 24h assay. PBMC of nine patients with metastasizing solid tumour failed to exert cytotoxicity against HEp-2 targets. In contrast, increased survival (decreased detachment from the monolayer) of HEp-2 cells was observed in the presence of PBMC from tumour patients that was significantly enhanced by addition of Con A during LDCC assay.
D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000097:blood / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D037102:Lectins