title: Location of the SH group of the alkali light chain on the myosin head as revealed by electron microscopy.
abstract: The location of the single cysteinyl residue of the alkali light chain on the myosin head was determined by electron microscopy. The cysteinyl residue of isolated alkali light chain 2 was biotinylated and the light chain was exchanged with that of heavy meromyosin in 4.7 M-NH4Cl. Avidin was attached to the biotin in the heavy meromyosin and the complex was rotary shadowed and observed in the electron microscope. The distance from the head-rod junction to the centre of avidin was 8(+/- 3) nm (mean value +/- standard deviation: n = 105).
mesh_terms: D000818:Animals; D001360:Avidin; D001665:Binding Sites; D003545:Cysteine; D008854:Microscopy, Electron; D015879:Myosin Subfragments; D009218:Myosins; D011817:Rabbits
chemical_list: D015879:Myosin Subfragments; D001360:Avidin; D009218:Myosins; D003545:Cysteine