Distribution and sub-cellular localization of drug metabolizing enzymes in the skin.
The distribution and sub-cellular localization of cytochrome P-450-dependent mono-oxygenase activity in the skin of adult hairless mice has been investigated using ethoxycoumarin, ethoxyresorufin and benzpyrene as substrates. Highest levels of mono-oxygenase activity per unit area of skin were found in the dermis, both before and after induction with benzanthracene. Subcellularly, mono-oxygenase activity was predominantly microsomal and, prior to induction, located entirely in the smooth microsomal fraction.
D015660:7-Alkoxycoumarin O-Dealkylase; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001551:Benz(a)Anthracenes / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008854:Microscopy, Electron; D008861:Microsomes / Q000648:ultrastructure; D010105:Oxygenases / Q000378:metabolism*; D012867:Skin / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
D001551:Benz(a)Anthracenes; C030935:benz(a)anthracene; D010105:Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; D001189:Aryl Hydrocarbon Hydroxylases