title: [Detection of toxoplasma-specific IgM antibodies--comparison with the ISAGA (immunosorbent agglutination assay) and immunofluorescence results].
abstract: Serum samples from 702 persons were examined for Toxoplasma-specific IgM-antibodies using the immunosorbent agglutination assay (ISAGA) and the immunofluorescence-test (IIFT). 250 samples showed a positive reaction in the Sabin-Feldman-test (SFT) with titers greater than or equal to 1: 1 024, 58% were positive in ISAGA and 36% in the IIFT. Samples of persons with acute Toxoplasma-infection, showed high titers in the ISAGA and SFT as well, even in cases where the IgM-IIFT was negative. SFT-negative sera and others with weakly-positive reactions and also rheuma-positive samples were negative in the ISAGA. It is discussed how far it is possible to determine the duration of the Toxoplasma infection by applying the ISAGA in combination with other antibody tests.
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D007163:Immunosorbent Techniques; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology*
chemical_list: D000906:Antibodies; D007075:Immunoglobulin M
vernacular_title: Untersuchungen zum Nachweis von Toxoplasma-spezifischen IgM-Antikörpern--Vergleich von ISAGA (Immunosorbent Agglutination Assay)- und Immunofluoreszenz-Ergebnissen.