Interleukin 2 induces synthesis of polypeptides in an interleukin 2-dependent cell line by transcription and translation.
Interleukin 2 (IL 2) is a lymphocyte growth factor capable of inducing the rapid growth of antigen- or mitogen-stimulated T cells. To understand the molecular mechanisms of IL 2 action, we studied the synthesis of cellular polypeptides induced by IL 2. For a homogeneous cell population responsive to IL 2, we used a murine clone of natural killer-like cells, NK 7, which is strictly dependent on exogenous IL 2 for cell viability and growth. NK 7 cells were first kept in culture without IL 2 for 6 h and then labeled for 3 h with [14C]leucine in the presence or absence of 100 U/ml recombinant human IL 2. Analysis of the cellular polypeptides by two dimensional gel electrophoresis and autoradiography revealed that the addition of IL 2 to IL 2-starved NK 7 cells specifically increased the levels of at least ten polypeptides (designated from molecular weight as p94a, p94b, p52, p51a, p51b, p48.5, p36, p34.5, p25.5, and p23). The increases of p52, p25.5, and p23 were inhibited by 5 micrograms/ml actinomycin D, whereas the other seven were virtually unaffected by the drug.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D000090266:Chromobox Protein Homolog 5; D002999:Clone Cells; D006801:Humans; D007376:Interleukin-2 / Q000302:isolation & purification / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics* / Q000302:isolation & purification; D014176:Protein Biosynthesis / Q000187:drug effects*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
C000715594:CBX5 protein, human; D007376:Interleukin-2; D010455:Peptides; D011994:Recombinant Proteins; D000090266:Chromobox Protein Homolog 5