title: Separate resistances to arsenate and arsenite (antimonate) encoded by the arsenical resistance operon of R factor R773.
abstract: The arsenical resistance operon of R factor R773 was analyzed by subcloning and insertional inactivation. The operon was found to have two functional regions, the promoter-proximal region encoding resistance to arsenite and antimonate and the promoter-distal region encoding arsenate resistance. A unique 1.6-kilobase fragment was shown to be sufficient to encode arsenate resistance and produce arsenate extrusion from intact cells.
mesh_terms: D001149:Arsenates / Q000378:metabolism / Q000494:pharmacology*; D001151:Arsenic / Q000378:metabolism / Q000494:pharmacology*; D018053:Arsenites; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D009154:Mutation; D009876:Operon; D010452:Peptide Biosynthesis; D011815:R Factors
chemical_list: D001149:Arsenates; D018053:Arsenites; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid