Variables that regulate production of insulin-like peptide(s) in human leukemia cell line (HL-60).
A human myeloid leukemia cell line (HL-60) produces a peptide or peptides with insulin-like activity which is distinct from insulin or insulin-like growth factors (somatomedins). Factors regulating the production of this peptide (HL-ILP) were explored in the present study. The production of HL-ILP was maximal in the early log phase of cell growth and declined with increasing cell density. Differentiation of HL-60 cells to macrophages, induced by dihydroxyvitamin D3 or phorbol esters, was also associated with a decrease in HL-ILP production. Glucose consumption by the cells in the early log phase was closely related with HL-ILP production, and HL-ILP was found to stimulate glucose consumption by HL-60 cells. Production of HL-ILP was dependent on glucose concentrations in the culture medium and glucose concentrations higher than 1mg/d1 suppressed the release of HL-ILP. These observations are not inconsistent with a hypothesis that HL-ILP is involved in the glucose metabolism of the HL-60 cells that produce this peptide.
D002117:Calcitriol / Q000494:pharmacology; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000096:biosynthesis* / Q000494:pharmacology; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000494:pharmacology; D013002:Somatomedins / Q000096:biosynthesis* / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007328:Insulin; D010455:Peptides; D013002:Somatomedins; D002117:Calcitriol; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate