Steroid hormone regulation of prostatic acid phosphatase expression in cultured human prostatic carcinoma cells.
We have investigated the modulation of prostatic acid phosphatase expression in the human prostatic cancer cell line LNCaP in response to the natural androgens testosterone and dihydrotestosterone, the female sex steroid estradiol and the synthetic androgen R1881 (methyltrienolone). Testosterone and dihydrotestosterone at 1 microgram/ml enhance the acid phosphatase synthesis by a factor of 3.5, while a hundred-fold lower concentration of the synthetic androgen R1881 induces an almost five-fold increase in the expression of this enzyme. The stimulation by all androgens tested and estradiol was dose-dependent. The synthetic glucocorticoid triamcinolone acetonide does not modulate the prostatic acid phosphatase expression in LNCaP cells, neither alone nor in combination with R1881.
D000135:Acid Phosphatase / Q000378:metabolism*; D002478:Cells, Cultured; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004963:Estrenes / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000502:physiology*; D006801:Humans; D008297:Male; D015741:Metribolone; D011467:Prostate / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology*; D013268:Stimulation, Chemical; D013739:Testosterone / Q000494:pharmacology; D014222:Triamcinolone Acetonide / Q000494:pharmacology
D004963:Estrenes; D012739:Gonadal Steroid Hormones; D013196:Dihydrotestosterone; D015741:Metribolone; D013739:Testosterone; D004958:Estradiol; D000135:Acid Phosphatase; D014222:Triamcinolone Acetonide