A simple method for high resolution light microscopy of nervous tissue.
A simple method for high resolution light microscopy of nervous tissue is described. Tissue perfused with glutaraldehyde and formaldehyde and postfixed with osmium tetroxide is embedded in HistoResin (LKB Bromma), a new glycol methacrylate based embedding medium, and sectioned (2 microns) on a standard microtome. The method gives excellent preservation of tissue structure, which is far superior to that in paraffin sections and in fact resembles that in epoxy resin-embedded material. It does not, however, require the time, expertise and ultramicrotome needed to cut epoxy resin sections. This technique is particularly useful for assessing demyelination.
D000818:Animals; D048588:Benzoxazines; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology; D008689:Methacrylates; D008853:Microscopy / Q000379:methods*; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D009417:Nerve Tissue / Q000033:anatomy & histology* / Q000473:pathology; D010078:Oxazines; D051381:Rats; D011917:Rats, Inbred Lew; D014048:Tolonium Chloride
D048588:Benzoxazines; D008689:Methacrylates; D010078:Oxazines; D014048:Tolonium Chloride; C028911:cresyl violet; C005044:hydroxyethyl methacrylate