Correction factors for the diphenylamine test for deoxyribonucleic acid in yeasts.
The diphenylamine assay for DNA content of yeast is unsatisfactory due to the presence of a yeast component which inhibits the colour reaction. The inhibitor is found in a wide range of yeast species and has not been identified. The relationships between the degree of inhibition and cell concentration, temperature and time of hydrolysis and extraction have been described. A formula for correction of the inhibition has been derived.
D000814:Aniline Compounds; D000818:Animals; D004271:DNA, Fungal / Q000032:analysis* / Q000378:metabolism; D003839:Deoxyadenosines / Q000032:analysis; D004159:Diphenylamine; D006868:Hydrolysis; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors; D015003:Yeasts / Q000032:analysis / Q000235:genetics
D000814:Aniline Compounds; D004271:DNA, Fungal; D003839:Deoxyadenosines; D004159:Diphenylamine