Analysis of B-cell subpopulations. I. Relationships among splenic xid, Ly 1+, and Lyb 5+ B cells.
The phenotypic and functional relationships among the various B-cell subsets is of importance for better understanding studies of the immune system. In this report, the realm of B cells encompassed by Lyb 5, Ly 1, and xid has been examined through the use of the alloantiserum anti-Lyb 5, the unique functional properties of Ly 1+ B cells, and the spontaneous autoantibody producing congenic xid mice, NZB.xid. By functional and phenotypic analysis, we have shown that (i) Lyb 5- B cells and B cells of xid mice are largely, but not completely, overlapping; (ii) xid spleen cells contain a population which is Lyb 5+; (iii) Ly 1+ B cells fall largely in the Lyb 5+ compartment; and (iv) the autoantibody-producing Ly 1+ B cells are predominantly Lyb 5+.
D000818:Animals; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly / Q000032:analysis* / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology; D005260:Female; D007136:Immunoglobulins / Q000096:biosynthesis; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D007518:Isoantibodies; D051379:Mice; D008808:Mice, Inbred CBA; D008814:Mice, Inbred NZB; D010641:Phenotype; D013154:Spleen / Q000166:cytology*
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D001323:Autoantibodies; D007136:Immunoglobulins; D007518:Isoantibodies; C029805:Lyt antibodies