Inhibition of DNA synthesis by chromium compounds.
Cr(VI) irreversibly inhibited DNA synthesis in cultured mouse L cells to 50% of controls at 10 microM; 3.3 mM Cr(III) did not. At 0.3 mM, Cr(III) and Cr(VI) inhibited DNA synthesis in permeabilized L cells to 50% of control values. Cr(III) was a stronger inhibitor of DNA synthesis in the DNA-Escherichia coli DNA polymerase I system than was Cr(VI). The inhibitory effect of Cr(VI) depended on the ratio of Cr/DNA and Cr/enzyme; on the other hand, the increase in the concentration of DNA polymerase did not affect the inhibition of Cr(III), Cr(III), below the inhibitory concentration, produced an increase in the incorporation of [3H]dTMP into DNA; this was not observed with Cr(VI).
D000818:Animals; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D002712:Chlorides; D002840:Chromates / Q000633:toxicity*; D002857:Chromium / Q000633:toxicity*; D017608:Chromium Compounds; D004247:DNA / Q000096:biosynthesis*; D004256:DNA Polymerase I / Q000378:metabolism; D004926:Escherichia coli; D007739:L Cells / Q000187:drug effects*; D051379:Mice; D017680:Potassium Compounds; D013329:Structure-Activity Relationship; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002712:Chlorides; D002840:Chromates; D017608:Chromium Compounds; D017680:Potassium Compounds; D002857:Chromium; D014316:Tritium; C027373:potassium chromate(VI); D004247:DNA; D004256:DNA Polymerase I; C022990:chromic chloride; D013936:Thymidine