Inhibition of in vitro anti-DNA B-cell responses by cyclosporine.
The action of the immunosuppressive agent cyclosporine (CsA) on anti-DNA B-cell responses was investigated in an in vitro system. Spleen cells from autoimmune MRL-lpr/lpr or control BALB/c mice, when cultured at high cell density, spontaneously produced significant amounts of IgM and IgG, including anti-DNA. IgG levels, both total and anti-DNA, were much higher for MRL-lpr/lpr cells compared to BALB/c cells, suggesting similarity of this model system to the in vivo response. For cells of both strains, the production of IgM and IgG anti-DNA was 10- to 100-fold more sensitive to the inhibitory activity of CsA than total immunoglobulin production. The effect was not manifest, however, in cultures stimulated with the B-cell mitogen, lipopolysaccharide. These observations suggest that CsA in certain dose ranges preferentially inhibits anti-DNA production, with efficacy determined by the mechanisms promoting B-cell hyperactivity.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001323:Autoantibodies / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D003524:Cyclosporins / Q000494:pharmacology*; D004247:DNA / Q000276:immunology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000187:drug effects
D001323:Autoantibodies; D003524:Cyclosporins; D004247:DNA