Control of human T-colony formation by interleukin-2.
T-colony formation can be induced in PHA-stimulated peripheral blood mononuclear cells (PBM) from man, but not in PHA-stimulated purified T cells, the latter requiring the presence of factors produced by PHA-stimulated PBM and termed T-colony promoting activity (TCPA). In this paper, we demonstrate that interleukin-2 (IL-2), the growth hormone of T lymphocytes, controls T-colony formation. We show that: IL-2 activity and TCPA produced by PHA-activated PBM are co-purified by gel filtration and chromatography on blue agarose, a procedure which yields a 850-fold IL-2 purification; recombinant IL-2, produced by genetically manipulated Escherichia coli, can induce T-colony formation in PHA-stimulated purified T cells; Monoclonal antibody against the IL-2 receptor (anti-Tac antibody) completely inhibits the T-colony formation in PHA-stimulated PBM when directly added to the culture system.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002850:Chromatography, Gel; D002999:Clone Cells; D006801:Humans; D007376:Interleukin-2 / Q000302:isolation & purification / Q000502:physiology*; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D010835:Phytohemagglutinins