title: L3T4-positive T lymphoblasts are responsible for transfer of immunity to Trichinella spiralis in mice.
abstract: The characteristics of lymphocyte subpopulations involved in mediating immunity to the intestinal nematode Trichinella spiralis in vivo have been examined using adoptive transfer in conjunction with accurate cell-sorting and cell-depletion techniques. Positive selection of cell subsets, using FACS sorting and velocity sedimentation at unit gravity, confirm that rapidly dividing T blasts are the major population that mediates expulsion of the worm from the gut. Furthermore, cell-depletion studies demonstrated that the T-cell subset involved is of the L3T4 + ve Lyt 2-ve phenotype. This phenotype suggests class II MHC restriction in recognition of T. spiralis antigens by T cells in vivo. The roles that such T cells play in immunity to T. spiralis are discussed in terms of lymphokine release.
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D002469:Cell Separation; D005434:Flow Cytometry; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth