An enzyme immunoassay for rapid isotyping of monoclonal antibodies.
An enzyme-immunoassay has been developed for rapid isotyping of murine monoclonal antibodies. This assay has several advantages over the commonly used typing methods. It does not require the presence of specific antigen. Typing plates can be prepared in advance and stored frozen. Unequivocal results can be obtained in 2 h, and the urease indicator system produces a vivid colour change that can be read visually.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice
D000911:Antibodies, Monoclonal