title: Epidermal growth factor or serum stimulation of rat fibroblasts induces an elevation in mRNA levels for lactate dehydrogenase and other glycolytic enzymes.
abstract: We have isolated cloned cDNAs corresponding to five mRNAs whose level is increased following stimulation of quiescent rat fibroblasts by either epidermal growth factor or serum. Partial sequencing followed by a computer search of data banks has shown that the cloned cDNAs correspond to mRNAs encoding proteins with extensive homology to lactate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase, enolase, triose phosphate isomerase, and actin. The complete nucleotide sequence of a rat fibroblast lactate dehydrogenase is presented.
mesh_terms: D000818:Animals; D001483:Base Sequence; D001769:Blood; D002645:Chickens; D004247:DNA / Q000032:analysis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D006019:Glycolysis; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D004247:DNA; D007770:L-Lactate Dehydrogenase