2-Mercaptoethanol-dependent lipopolysaccharide-responsive B cells in nylon-wool-fractionated spleen cell preparations.
The ability of lipopolysaccharide (LPS) to influence lymphocyte mitogen responsiveness to 2-mercaptoethanol (2-ME) was investigated. 2-ME was mitogenic for splenocytes only when fetal bovine serum lots containing elevated endotoxin levels were used to supplement (5%) the culture medium. 2-ME did not stimulate mitosis of nylon-wool-fractionated splenocytes (NWSC); however, 2-ME induced significant proliferation in the NWSC cell preparations when endotoxin-deficient culture medium was supplemented with doses of LPS which alone did not stimulate significant proliferation. The sIg+ and Thy-1-/Lyt-1- fractions of NWSC, which represented less than 5% of the cells in the 'T-cell preparations', were almost completely dependent on 2-ME for proliferation in response to LPS stimulation. These data indicate that nylon-wool-fractionated preparations contain splenic B cells that incorporate significant amounts of [3H]thymidine when stimulated with LPS and 2-ME.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008623:Mercaptoethanol / Q000494:pharmacology*; D051379:Mice; D009757:Nylons; D013154:Spleen / Q000166:cytology
D008070:Lipopolysaccharides; D009757:Nylons; D008623:Mercaptoethanol