Eosinophil activation by lymphokines and T cell clone products in the rat.
T cell lines and T cell clones derived from inbred Fischer rats and specific for Schistosoma mansoni antigens were established. Cell-free supernatants from the T cell lines demonstrated a marked capacity to enhance IgE- and IgG2a-dependent eosinophil-mediated killing of S. mansoni larvae in vitro. In addition, supernatants from cloned T cells stimulated with concanavalin A or specific antigen, or unstimulated, enhanced IgE-dependent eosinophil-mediated helminthotoxicity. The enhancing activity in both cases was very heat-stable (100 degrees C, 10 min). We also found that clone-derived supernatants enhance eosinophil peroxidase release upon stimulation with homologous IgE and anti-IgE as well as inducing a more delayed spontaneous release of peroxidase. In view of the established thymus dependency for the development of immunity to schistosomiasis in the rat, the availability of these S. mansoni-specific cloned T cells has enabled the relationship between eosinophils, lymphocytes and anaphylactic antibodies to be examined more closely.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000947:Antigens, Helminth / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D006358:Hot Temperature; D007114:Immunization; D066298:In Vitro Techniques; D008222:Lymphokines / Q000276:immunology*; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D010544:Peroxidases / Q000378:metabolism; D051381:Rats; D012550:Schistosoma mansoni / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000947:Antigens, Helminth; D008222:Lymphokines; D010544:Peroxidases