title: Enhancement of antibody response by mouse dendritic cells pulsed with tobacco mosaic virus or with rabbit antiidiotypic antibodies raised against a private rabbit idiotype.
abstract: The role of splenic lymphoid dendritic cells (DC) and macrophages (M phi) from mice in induction of immune responses in vivo has been investigated. Varying numbers of purified DC and M phi pulsed in vitro with tobacco mosaic virus (TMV) or with rabbit antiidiotypic antibodies (Ab2) directed against a private rabbit anti-TMV idiotype were injected back into syngeneic mice. In both systems, DC appeared to strongly enhance the primary and secondary responses to the virus. Optimal responses were obtained with 5 X 10(4) purified DC carrying TMV or rabbit Ab2. In contrast, M phi were less efficient by a factor of at least 100. These results show the potency of lymphoid DC as inducing cells in T-dependent antibody responses in vivo.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007156:Immunologic Memory; D008264:Macrophages / Q000276:immunology; D051379:Mice; D011817:Rabbits; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014027:Tobacco Mosaic Virus / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007130:Immunoglobulin Idiotypes