Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.
cDNAs encoding the mRNAs for the fusion protein (F) and the hemagglutinin/neuraminidase protein (HN) of the paramyxovirus simian virus 5 have been inserted into a eukaryotic expression vector under the control of the simian virus 40 late promoter. The F and HN proteins synthesized in recombinant infected cells are indistinguishable in terms of electrophoretic mobility and glycosylation from the proteins synthesized in simian virus 5-infected cells. In addition, the expressed F and HN proteins have been shown to be anchored in the plasma membrane in a biologically active form by indirect live cell immunofluorescence, the F-mediated formation of syncytia, and the ability of HN to cause the hemadsorption of erythrocytes to the infected cell surface.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D006389:Hemagglutinins, Viral / Q000096:biosynthesis*; D009439:Neuraminidase / Q000096:biosynthesis*; D010252:Paramyxoviridae / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000096:biosynthesis*; D014760:Viral Fusion Proteins
D006389:Hemagglutinins, Viral; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D004247:DNA; D009439:Neuraminidase