[Production of GM-CSF by human T lymphocytes purified and stimulated by Con A].
Production of granulomacrophage colony stimulating factor (GM-CSF) by human T lymphocytes has not yet been demonstrated due to insufficient monocyte depletion in cell suspensions. We studied GM-CSF production from normal blood T lymphocyte suspensions containing less than 0.1% of monocytes; moreover, human bone marrow samples were also depleted of adherent cells. A colony stimulating activity (CSA) was always found in the 11 healthy subjects studied following Con A stimulation. The colonies were principally of the granulocytic type. These results strongly suggest that normal human T cells have the capacity to produce CSA.
D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D003208:Concanavalin A / Q000494:pharmacology*; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D008264:Macrophages / Q000166:cytology; D009000:Monocytes / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D003208:Concanavalin A
Production de GM-CSF par les lymphocytes T humains purifiés et stimulés par la Con A.