Partial purification and biochemical characterization of a T cell suppressor factor produced by human glioblastoma cells.
The human glioblastoma cell line 308 constitutively secretes a soluble factor with biologic and biochemical characteristics of human monocyte-derived interleukin 1 (IL 1). The 308 cells also produce a 97,000 m.w. factor that inhibits the effects of IL 1 and interleukin 2 (IL 2) on T lymphocytes. By using sequential chromatography on Blue Affigel, hydroxyapatite, and Ultrogel AcA54, the inhibitory factor, termed glioblastoma-derived T cell suppressor factor (G-TsF), was separated from IL 1 and purified 2000-fold with respect to the protein present in the crude 308 cell supernatant. This G-TsF preparation was sensitive to tryptic proteolysis, showed a peak of pI 4.6 on isoelectric focusing, and when labeled with 125I, revealed six protein bands in the range of 30 to 100 kdaltons on SDS gel.
D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005910:Glioma / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007164:Immunosorbents; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000302:isolation & purification / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D007164:Immunosorbents; D007457:Iodine Radioisotopes; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic; D014357:Trypsin