Dialysis-induced transformation of mouse submandibular androgen-receptor.
Dialysis induced transformation of cytosol androgen receptor from mouse submandibular gland. On DEAE-Sephacel chromatography, this dialyzed [3H]methyltrienolone receptor complex was eluted at 0.10 M KCl which was lower than 0.25 M KCl required to elute the nontransformed androgen receptor complex, but higher than 0.05 M KCl required to elute the heat transformed receptor. On glycerol density gradient centrifugation, the dialysis transformed receptor complex shifted its sedimentation coefficient to 6 S from 8 S of the nontransformed condition, whereas the heat transformed receptor was sedimented at 4 S. Molybdate inhibited the dialysis-induced transformation on DEAE-Sephacel chromatography. The charge and the molecular size of dialysis-induced transformed receptor complex were different from those of heat-induced transformed receptor complex.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D003600:Cytosol / Q000378:metabolism; D003956:Dialysis; D004963:Estrenes / Q000378:metabolism; D005260:Female; D006358:Hot Temperature; D015741:Metribolone; D051379:Mice; D008970:Molecular Weight; D009994:Osmolar Concentration; D011944:Receptors, Androgen / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*
D004963:Estrenes; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D015741:Metribolone