Modulation of cellular phosphoprotein profiles in transformation and redifferentiation of murine AKR embryonic fibroblastic cells.
Cellular phosphoprotein profiles from normal mouse embryonic fibroblast AKR-2B cells were compared to those of their permanently, chemically transformed malignant counterparts AKR-MCA cells, and AKR-2B cells reversibly transformed by transforming growth factor (AKR-TGF). Similar 32P-phosphorylation profiles were observed for both the AKR-TGF and AKR-MCA cells which were distinct from that of the normal AKR-2B cells. Dimethylformamide (DMF)-induced differentiation of the AKR-MCA cells resulted in a restoration of the normal AKR-2B phosphorylation profile to the malignant AKR-MCA cells.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004126:Dimethylformamide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011247:Pregnancy; D015291:Transforming Growth Factors
D010455:Peptides; D010750:Phosphoproteins; D015291:Transforming Growth Factors; D004126:Dimethylformamide