Influence of LH, hCG and PRL on steroid release from granulosa cells of early pregnant and pseudopregnant sows.
Granulosa cells were isolated from porcine ovaries on the 18th day of pregnancy or pseudopregnancy. Pseudopregnancy was induced applying estradiol benzoate (EB). The isolated cells were cultured for 4 days at 37 degrees C in Medium 199 with an addition of 15% calf serum. Studies were performed on the effect of the following hormones upon estradiol 17 beta (E2) and progesterone (P4) secretion: 10 and 100 ng LH, 10 and 100 ng PRL, and 0.01, 0.1 and 1 U hCG/ml of the medium. Concentration of E2 and P4 was determined in the medium after 2 and 4 days of culture by radioimmunoassay. It was shown that hCG at a dose of 0.01 U increased E2 release by the granulosa cells obtained from pregnant sows. None of the hormones under study affected E2 secretion after EB treatment. Moreover P4 secretion by the porcine granulosa cells was about twice higher in EB treated pigs than in the pregnant animals. The cells from pseudopregnant sows were more susceptible to the applied exogenous hormones: LH, hCG and PRL (only 100 ng dose) increased P4 secretion. Contrarily to this, the granulosa cells from pregnant pigs increased P4 secretion only under the effect of 1 U hCG.
D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D007986:Luteinizing Hormone / Q000494:pharmacology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D011555:Pseudopregnancy; D013552:Swine
D006063:Chorionic Gonadotropin; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone