title: Studies on the "labile-bound" glucose compartment in erythrocytes: studies on Psammomys obesus (sand rat) and preliminary studies on human erythrocytes.
abstract: Enzymatic (glucose oxidase) measurement of glucose concentration in the fluid compartment of Psammomys erythrocytes (Gfe) and of its concentration in the fluid compartment of blood plasma (Gfp) gives the ratio (mean +/- SD): Gfe/Gfp = 1.50 +/- 0.43 (n = 12, 23 degrees C). However, when we added 3H-labeled glucose (G*) in vitro to the whole blood, the ratio after 2 min was G*fe/G*fp = 0.90 (SD 0.11) and after 5 min G*fe/G*fp = 0.97 (SD 0.12). These calculations were based on previous determination of the fractional volumes of the fluid and non-fluid compartments in Psammomys blood. The results suggest that there is more than one compartment of measurable glucose in Psammomys erythrocytes. Glucose undergoes a fast free transfer between the plasma and the erythrocyte fluids, and a much slower transmission to another measurable compartment in the erythrocyte, where it is loosely bound to other molecules. This loosely bound glucose does not participate in the fast kinetic transmission across the erythrocyte membrane, but it is measurable by the glucose-oxidase-based method. Preliminary studies on human erythrocytes lead to similar conclusions.
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000097:blood*; D001786:Blood Glucose / Q000032:analysis*; D002415:Cats; D002417:Cattle; D002451:Cell Compartmentation; D004285:Dogs; D004912:Erythrocytes / Q000166:cytology*; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D008253:Macaca mulatta; D008433:Mathematics; D011817:Rabbits; D012756:Sheep; D013045:Species Specificity; D013552:Swine
chemical_list: D001786:Blood Glucose