Signal requirements for growth and differentiation of activated murine B lymphocytes.
Mouse B lymphocytes were stimulated at high cell concentrations with goat anti-IgM antibodies, which leads to the induction of B cell proliferation without the addition of any growth factors. After 48 hr, blast cells were purified and cultured at low cell concentrations. Proliferation and differentiation of purified B lymphocyte blasts is then dependent on the addition of either mitogens (e.g., LPS) or certain lymphokines derived from activated T cells or macrophages. One such lymphokine was isolated from supernatants of various activated T cells and characterized by gel filtration as a material with an apparent m.w. of 40,000 to 50,000, similar to BCGF II. It supports the proliferation of the B cell blasts and induces their differentiation into plaque-forming cells. Lymphokines such as BCGF I, interleukin 2, and BCDF gamma could neither maintain growth nor induce differentiation of B lymphocytes preactivated by goat anti-IgM.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation; D002455:Cell Division; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006133:Growth Substances / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000378:metabolism
D006133:Growth Substances; D008222:Lymphokines; D015847:Interleukin-4