Expression of a monocyte-specific esterase isoenzyme in cases of acute myeloid leukemias.
The carboxylic esterase (E.C. 3.1.1.1) isoenzymes from cases of acute myeloid leukemias were separated by analytical isoelectric focusing on horizontal thin-layer gels. One isoenzyme consisting of one or two components (bands) could be completely and selectively inhibited by addition of 40 mM sodium fluoride (NaF) to the staining bath. The 105 cases were classified into the groups M1-M6 according to the FAB proposals. The NaF-sensitive isoenzyme was not detected in cases of FAB groups M1/2 (acute myeloblastic leukemia without or with maturation), group M3 (acute promyelocytic leukemia) or group M6 (erythroleukemia). Thirty-one out of 33 cases in the FAB group M4 (acute myelomonocytic leukemia) and 9/9 cases in FAB group M5 (acute monocytic leukemia) expressed the NaF-sensitive isoenzyme. The NaF-sensitive isoenzyme was found at different staining intensities; all M5 cases showed the isoenzyme at strong or very strong intensity, whereas most of the M4 cases displayed the isoenzyme at weak, medium or strong staining intensity. The data presented are further evidence that the presence of the NaF-sensitive esterase isoenzyme indicates monocytic involvement or differentiation in cases of myeloid leukemias. The easy and fast to perform method of isoelectric focusing can be used to distinguish the monocytic variants among the acute myeloid leukemias and can supplement the morphological analysis of these cases.
D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology* / Q000473:pathology; D009000:Monocytes / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology
D007527:Isoenzymes; D012969:Sodium Fluoride; D002265:Carboxylic Ester Hydrolases