Transient expression of glial fibrillary acidic protein in developing oligodendrocytes in vitro.
Expression of galactocerebroside (GC) and glial fibrillary acidic protein (GFAP) was studied in oligodendrocyte-enriched cultures of newborn mouse brains. In the cultures, GC was detectable as early as 1 day in vitro (1-DIV). Using double immunofluorescence labeling, some GC-positive cells were also stained homogeneously by the anti-GFAP serum. The intensity of GFAP staining increased until 9-DIV when the GC-positive cells revealed the typical morphology of oligodendrocytes, and the GFAP staining faded thereafter. The GFAP staining pattern of the GC-positive cells was not changed upon exposure to demecolcine, even though the GC-negative, GFAP-positive astrocytes showed perinuclear aggregation of GFAP. No intermediate filaments were observed in the oligodendrocytes by electron microscopy. The results suggest that the oligodendrocytes may have soluble GFAP in a certain period of early development.
D000818:Animals; D000831:Animals, Newborn; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009457:Neuroglia / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*
D005904:Glial Fibrillary Acidic Protein