Suppression of antibody response to transfusions in rats by preconditioning with antibody-coated cells.
In a Lewis-BN rat model, the antibody response to transfused blood cells from strongly histoincompatible donors was suppressed by preincubation of blood with specific antidonor antiserum or broadly reactive heterologous antilymphocyte serum (ALS). Suppression was achieved even when excess antiserum was removed by washing prior to injection. The suppressive effect was dose-dependent. Broadly reactive ALS was even more effective than specific antidonor antiserum in inhibiting the primary antibody response. Lewis animals pretreated three times with ALS-coated BN blood showed no secondary antibody response against subsequent BN-blood booster injections. Our experiments may be relevant for the prevention of undesired sensitization to donor-specific transfusions prior to related donor kidney transplantation.
D000818:Animals; D000917:Antibody Formation; D000961:Antilymphocyte Serum / Q000494:pharmacology; D006648:Histocompatibility; D007108:Immune Tolerance / Q000187:drug effects; D007117:Immunization, Secondary; D017710:Lymphocyte Transfusion; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D065227:Transfusion Reaction
D000961:Antilymphocyte Serum