Anti-neuroblastoma monoclonal antibodies which do not bind to bone marrow cells.
Three previously unreported mouse monoclonal antibodies are described. All three bind to cultured human neuroblastoma cells from six of six cell lines, but do not detectably bind to human bone marrow cells. The two IgG2a monoclonal antibodies bind to greater than 98% of the cultured cells from each cell line, bind to six of six human neuroblastoma tumors by immunoperoxidase histochemistry, and are cytotoxic with complement to cultured human neuroblastoma cells but not to human bone marrow granulocyte-macrophage colony-forming cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000920:Antibody-Dependent Cell Cytotoxicity; D001666:Binding Sites, Antibody; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D002470:Cell Survival; D002648:Child; D002675:Child, Preschool; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology; D009447:Neuroblastoma / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009194:Myeloma Proteins; D003165:Complement System Proteins