Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains.
Lipopolysaccharide-defective mutants of Salmonella typhimurium were transformed by plasmid DNA with a Ca2+ treatment method. Only those mutants with an Rc or Rd2 chemotype, due to galE or rfaF mutations, respectively, gave efficiencies greater than 10(5) transformants per microgram of DNA, frequencies 8- to 630-fold higher than with smooth strains or other rough mutants.
D002118:Calcium / Q000494:pharmacology; D002463:Cell Membrane Permeability; D008070:Lipopolysaccharides / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010957:Plasmids; D012486:Salmonella typhimurium / Q000235:genetics*; D014169:Transformation, Bacterial / Q000187:drug effects
D008070:Lipopolysaccharides; D002118:Calcium