Mass spectral analysis of murine epidermal growth factor.
Fast atom bombardment mass spectrometry has been used to characterize epidermal growth factor isolated from mouse submaxillary glands. The preparation is found to consist of two peptides, one of which has the average molecular weight predicted for the familiar gene product. The molecular weight of the second component is found to be reduced by the mass of one asparagine residue. These observations are discussed in light of previous reports of heterogeneity.
D000595:Amino Acid Sequence; D000818:Animals; D004220:Disulfides / Q000032:analysis; D004815:Epidermal Growth Factor / Q000302:isolation & purification; D046911:Macromolecular Substances; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008970:Molecular Weight; D013363:Submandibular Gland / Q000032:analysis
D004220:Disulfides; D046911:Macromolecular Substances; D004815:Epidermal Growth Factor