Interaction of 3-deaza-adenosine, a phospholipid methyltransferase inhibitor, on the production of pluripoietins from human peripheral T cells.
The formation of mixed colonies is dependent upon the addition of leucocyte-conditioned medium prepared with the mitogenic lectin phytohemagglutinin (PHA). The activation of peripheral T cells by PHA revealed an increase in methylated phospholipids and subsequently led to the release of stimulatory activities into the media. Stimulatory activities supporting mixed colony formation could not be detected when peripheral T cells were preincubated with the methyltransferase inhibitor 3-deaza-adenosine for 60 min before PHA stimulation. The addition of 3-deaza-adenosine to the culture at day 0 demonstrated no inhibitory effect on multilineage colony formation. The data suggest that the activation of human peripheral T cells from healthy volunteers by the mitogenic lectin PHA causes an increase in methylated phospholipids and might be an important signal for the mitogenesis of T-lymphocytes, which subsequently lead to the production of stimulatory activities promoting the growth of pluripotent stem cells.
D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000096:biosynthesis* / Q000378:metabolism; D008780:Methyltransferases / Q000037:antagonists & inhibitors; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase; D050918:Phosphatidylethanolamine N-Methyltransferase; D010835:Phytohemagglutinins / Q000494:pharmacology; D012263:Ribonucleosides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000378:metabolism*; D014372:Tubercidin / Q000494:pharmacology*
D008222:Lymphokines; D010835:Phytohemagglutinins; D012263:Ribonucleosides; C018258:3-deazaadenosine; D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase; D014372:Tubercidin