Stimulation of 3-O-methylglucose transport in adipocytes by a synthetic human growth hormone fragment.
The insulin-like effects of intact human growth hormone (hGH) were demonstrated with a synthetic amino-terminal fragment of the molecule, containing the sequence Leu-Ser-Arg-Leu-Phe-Asp-Asn-Ala (hGH 6-13). The in vitro study on the molecular mechanism of the hypoglycaemic action revealed that hGH 6-13 enhanced the transport of 3-O-methylglucose in adipocytes independently of its ability to potentiate the binding of insulin to the isolated cells. Current results suggest that the hGH part-sequence either stimulated ligand-binding to intact cells, subsequently amplifying the membrane regulatory functions, or promoted a biochemical event common to the binding of insulin and concanavalin A, resulting in the increase of transport and utilization of glucose.
D019325:3-O-Methylglucose; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D008297:Male; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D008757:Methylglucosides; D008759:Methylglycosides; D010446:Peptide Fragments; D003208:Concanavalin A; D019325:3-O-Methylglucose; C037849:somatotropin (6-13); D013006:Growth Hormone