title: A muscle-type tropomyosin in human fibroblasts: evidence for expression by an alternative RNA splicing mechanism.
abstract: We have isolated a cDNA clone from a human fibroblast cDNA library that contains the entire protein-coding region of a 1.1-kilobase mRNA. This mRNA encodes a 284-amino acid tropomyosin, the primary structure of which most closely resembles smooth muscle tropomyosin. Thus, the expression of both 284-amino acid muscle-type and 247-amino acid non-muscle-type tropomyosins appears to be a normal feature of human non-muscle cells. We also present evidence to suggest that this cytoskeletal tropomyosin and a human skeletal muscle beta-tropomyosin are derived from a common structural gene by an alternative RNA splicing mechanism.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003598:Cytoskeletal Proteins / Q000235:genetics; D005347:Fibroblasts / Q000502:physiology*; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D009124:Muscle Proteins / Q000235:genetics*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014335:Tropomyosin / Q000235:genetics*
chemical_list: D003598:Cytoskeletal Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D014335:Tropomyosin