Partial purification and characterization of a human lymphokine which induces antileishmanial activity in mouse macrophages.
Human blood mononuclear cells stimulated in vitro with concanavalin A secrete a lymphokine which activates mouse macrophages to kill Leishmania enriettii. Supernatants of cells cultivated for 1 day and for 2 days were analyzed for this activity after G-100 gel filtration and isoelectrofocusing. First day antileishmanial activity was found in a MW range of 23,000 with an isoelectric point of 3.6 to 4.0. Second day antileishmanial activity eluted in a peak in the MW range of 23,000 to 65,000 with an isoelectric point of 4.0 to 4.3. This activity could be completely separated from migration-inhibitory factor (MIF) after isoelectrofocusing in first day and in second day supernatants. Antileishmanial activity could be separated from interferon-gamma in first day, but not in second day supernatants.
D000981:Antiprotozoal Agents; D002850:Chromatography, Gel; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007113:Immunity, Innate; D007525:Isoelectric Focusing; D007700:Kinetics; D007891:Leishmania / Q000276:immunology*; D008222:Lymphokines / Q000302:isolation & purification* / Q000494:pharmacology; D008263:Macrophage Migration-Inhibitory Factors / Q000302:isolation & purification; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000276:immunology*; D013997:Time Factors
D000981:Antiprotozoal Agents; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D016215:Macrophage-Activating Factors