title: Escherichia coli mutant with altered respiratory control of the frd operon.
abstract: In wild-type Escherichia coli, fumarate reductase encoded by the frd operon is inducible by its substrate in the absence of molecular oxygen and nitrate. Synthesis of this enzyme under permissive conditions requires the fnr+ gene product, which is believed to be a pleiotropic regulatory protein that activates transcription. A spontaneous mutant was isolated in which the expression of the frd operon no longer depended on the presence of fumarate or the fnr+ gene product. Aerobic repression of the operon was abolished, but nitrate repression remained intact. Transductional analysis showed that the mutation was closely linked to the frd locus. The mutant phenotype strongly suggests that repression by molecular oxygen and nitrate is mediated by different mechanisms.
mesh_terms: D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005809:Genes, Regulator; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D009097:Multienzyme Complexes / Q000235:genetics*; D009154:Mutation; D009566:Nitrates / Q000494:pharmacology; D009876:Operon; D013385:Succinate Dehydrogenase / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D009566:Nitrates; D005993:Glycerolphosphate Dehydrogenase; C033727:fumarate reductase complex; D013385:Succinate Dehydrogenase