Typing Haemophilus ducreyi by indirect immunofluorescence assay.
Rabbits were inoculated with untreated whole cells of 16 strains of Haemophilus ducreyi. Homologous titres in an indirect immunofluorescence test ranged from 1/320 to 1/1280. No cross reactions, or very few, were observed between antisera to these strains and 22 other (possible taxonomically related) species, strains, and genera. A large diversity in types of antigen was observed among the strains of H ducreyi. Antiserum from two antigens (35000 and 3138), however, reacted with all 16 H ducreyi antigens tested. At least nine of the strains of H ducreyi examined could be identified as determining the type of antigen.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006191:Haemophilus ducreyi / Q000145:classification* / Q000276:immunology; D011817:Rabbits; D012703:Serotyping
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial