Amino acid sequence of Streptomyces metallo-proteinase inhibitor from Streptomyces nigrescens TK-23.
Streptomyces Metallo-Proteinase Inhibitor (S-MPI) consists of 102 amino acid residues, including one methionine and two disulfide bridges. The complete amino acid sequence of S-MPI, including two disulfide bridges, was determined by sequencing of tryptic and chymotryptic peptides of two fragments obtained by cyanogen bromide cleavage followed by reduction and S-pyridylethylation of the protein. Incubation of the inhibitor with thermolysin slowly cleaved one peptide bond, Cys(64)-Val(65), which might be a reactive site of S-MPI.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D004220:Disulfides / Q000032:analysis; D008666:Metalloendopeptidases; D010446:Peptide Fragments / Q000032:analysis; D011480:Protease Inhibitors; D013302:Streptomyces / Q000032:analysis*; D013820:Thermolysin / Q000378:metabolism; D014357:Trypsin
D000596:Amino Acids; D004220:Disulfides; D010446:Peptide Fragments; D011480:Protease Inhibitors; D002918:Chymotrypsin; D014357:Trypsin; D008666:Metalloendopeptidases; D013820:Thermolysin; D003488:Cyanogen Bromide