The influence of antimicrobial agents on macrophage-associated Staphylococcus aureus.
Macrophages obtained by culturing human blood monocytes were incubated with Staphylococcus aureus for phagocytosis to occur and exposed to gentamicin, rifampin, clindamycin or trimethoprim/sulphamethoxazole. The macrophage-associated bacteria were protected against gentamicin at low concentrations (1 mg/l) and trimethoprim/sulphamethoxazole. However, high concentrations of gentamicin and clindamycin reduced the number of bacteria, indicating that these drugs penetrated into human macrophages and killed phagocytosed bacteria. Rifampin, even at low concentrations (0.5 mg/l), caused a marked reduction in macrophage-associated bacteria, implying that the drug penetrated into the phagocytes and retained its effect in the cells most effectively.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002981:Clindamycin / Q000494:pharmacology; D004338:Drug Combinations / Q000494:pharmacology; D004347:Drug Interactions; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D010587:Phagocytosis; D012293:Rifampin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000276:immunology; D013420:Sulfamethoxazole / Q000494:pharmacology; D014295:Trimethoprim / Q000494:pharmacology; D015662:Trimethoprim, Sulfamethoxazole Drug Combination
D000900:Anti-Bacterial Agents; D004338:Drug Combinations; D005839:Gentamicins; D002981:Clindamycin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D014295:Trimethoprim; D013420:Sulfamethoxazole; D012293:Rifampin