Identity of the photoproduct that causes lacI mutations in UV-irradiated Escherichia coli.
Estimates of the capacity of photoreactivation to act specifically on premutational lesions were obtained by conjugational transfer of an F' lac plasmid from a UV-irradiated, photoreactivated donor to a delta (pro-lac) recipient that had been UV irradiated and allowed to induce SOS functions for 30 min. This treatment reduced the frequency of induced lacI mutations by 70 to 80%, indicating that cyclobutane dimers cause most mutations in this system.
D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects*; D007763:Lac Operon; D008027:Light; D009154:Mutation; D010957:Plasmids; D011938:Rec A Recombinases / Q000502:physiology
D011938:Rec A Recombinases