Early diagnosis of herpes simplex virus encephalitis in childhood by the enzyme-linked immunosorbent assay.
IgG antibody of cerebro-spinal fluid (CSF) and serum IgM antibody were determined by the enzyme-linked immunosorbent assay (ELISA) in 3 patients with herpes simplex virus encephalitis (HSVE). The ELISA detected the early rise of IgG antibody for herpes simplex virus (HSV) in CSF on the 3rd/4th day from onset of symptoms and IgM antibody in serum similarly on the 3rd/4th day, respectively. CSF albumin, IgG index and daily IgG synthesis during the early phase of HSVE showed significantly high levels. However, there was no significant increase of complement-fixing antibody and neutralizing antibody for HSV of serum and CSF in 2 of 3 patients. The ELISA turned out to be a more sensitive and convenient test for the early diagnosis of HSVE.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000134:cerebrospinal fluid; D000914:Antibodies, Viral / Q000032:analysis / Q000134:cerebrospinal fluid; D002675:Child, Preschool; D004660:Encephalitis / Q000175:diagnosis / Q000209:etiology*; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007231:Infant, Newborn; D008297:Male; D012699:Serology; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M