[Histological study and functional results of tubal and ovarian transplants in the rat (isografts and allografts treated with cyclosporin A)].
A microsurgical technique was used to transplant the tube and the ovary in the rat. 49 autografts were carried out (in a pure line of Lewis strain) to study histologically the results of cold ischaemia and the functional future of the graft in the form of pregnancies. 40 hybrid allografts Lewis-DA were transplanted in Lewis or DA rats. 19 animals that were not treated allowed us to study the phenomena of rejection histologically. 21 further rats were treated with Cyclosporin A to study their fertility. The histological results of temporary cold ischaemia are minimal and come down to atresia of the active antral follicles of the cohort. None of the histological structures of the tube seemed to suffer from cold ischaemia that lasted 20 minutes. The average size of the litter for the autografts was 6.4 baby rats with an index of implantation of 0.65. Where no treatment was given, allografts were very quickly rejected. Giving Cyclosporin A allowed the allografts to be tolerated in 60% of cases. 5.6 baby rats per litter were obtained with an index of implantation of 0.65. Cyclosporin A in an immunosuppressive drug which is not teratogenic in animals and is not cytostatic. It inhibits immune responses at the cellular level as well as the production of lymphocyte T dependent antibodies. This study shows again the value of transplants for treating tubo-ovarian sterility.
D000818:Animals; D003524:Cyclosporins / Q000627:therapeutic use*; D005187:Fallopian Tubes / Q000166:cytology / Q000637:transplantation*; D005260:Female; D006084:Graft Rejection; D007036:Hypothermia, Induced / Q000009:adverse effects; D007511:Ischemia / Q000503:physiopathology; D008098:Litter Size; D010053:Ovary / Q000166:cytology / Q000637:transplantation*; D011247:Pregnancy; D051381:Rats; D011917:Rats, Inbred Lew; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D003524:Cyclosporins
Etudes histologiques et devenir fonctionnel des greffes de trompe et d'ovaire chez la rate (isogreffes et allogreffes traitées par cyclosporine A).