Comparison of the API rapid E four-hour system with the API 20E overnight system for the identification of routine clinical isolates of the family Enterobacteriaceae.
Four hundred forty-one clinical isolates of the family Enterobacteriaceae were identified in parallel by using the API Rapid E 4-h and the API 20E overnight procedures (Analytab Products, Plainview, N.Y.). The results obtained by using the API Rapid E were compared with those obtained by using the API 20E. Discrepancies were resolved by using standard biochemicals. The API 20E identified 98.9% (436 of 441) of the isolates without the use of additional biochemicals and was found to be correct in each case of a discrepancy among the 436 isolates. The API Rapid E gave the same identification as the API 20E for 94.0% (410 of 436) of the isolates, misidentified 3.0% (13 of 436), and gave a correct but low-selectivity answer for the remaining 3.0% (13 of 436). The API Rapid E is a suitable alternative for the rapid identification of the Enterobacteriaceae.
D001431:Bacteriological Techniques; D003951:Diagnostic Errors; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D006801:Humans; D011934:Reagent Strips; D013997:Time Factors
D011934:Reagent Strips