Fractionated populations of normal human marrow cells respond to both human colony-stimulating factors with granulocyte-macrophage activity.
Populations of normal human colony-forming cells (blast cells) and cluster-forming cells (promyelocytes-myelocytes) were obtained from bone marrow by using the monoclonal antibody WEM G11 and the fluorescence-activated cell sorter (FACS). Both populations were shown to be responsive to both human colony-stimulating factors (CSFs) with granulocyte-macrophage activity (CSF alpha and CSF beta), with the cluster-forming cell population being more responsive to CSF beta than the colony-forming cell population. The clonal proliferation of promyelocytes-myelocytes was transient, and the clones generated were of subcolony size (less than 40 cells) regardless of the CSF used. Clone transfer experiments demonstrated that progeny of promyelocytes-myelocytes initiated using one stimulus (CSF alpha or CSF beta) were also responsive to the other stimulus.
D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells; D002455:Cell Division; D002469:Cell Separation; D002999:Clone Cells / Q000166:cytology; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D004804:Eosinophils / Q000166:cytology; D004912:Erythrocytes / Q000166:cytology; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors