Establishment and characterization of a B-cell line from a patient with acute lymphoblastic leukemia.
A permanent lymphoblastoid cell line was established from the peripheral blood of a child with acute lymphoblastic leukemia. The cell line, designated SDK, grows in a stationary suspension culture, forming aggregates, in RPMI medium supplemented with 10% FCS, with a doubling time of 50-60 h. Immunologic markers and cytological features suggested that the SDK cells should be identified as being of B-cell origin. The cells failed to form rosettes with sheep erythrocytes, did not express T-cell antigens as defined by monoclonal antibodies, and exhibited surface and cytoplasmic immunoglobulin determinants. Chromosome analysis revealed the presence of three cell populations with (a) 46XY; (b) t(8q-; 14q+) or 2p-; 14q+) and (c) cells with unidentifiable markers. SDK demonstrated susceptibility to TPA-induced differentiation toward plasma cells.
D000135:Acid Phosphatase / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology*; D002460:Cell Line; D002648:Child; D002869:Chromosome Aberrations; D006651:Histocytochemistry; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008297:Male; D009283:Naphthol AS D Esterase / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate
D009283:Naphthol AS D Esterase; D000135:Acid Phosphatase; D013755:Tetradecanoylphorbol Acetate