Anti-kinetochore antibodies: use as probes for inactive centromeres.
Application of a modified immunofluorescence technique using an anti-kinetochore serum enables cytogeneticists to obtain quality metaphase spreads and to localize kinetochores. In a patient with a 45, XX, -9, -11, tdic (9p;11p) constitution, we found that the dicentric marker chromosome has an intensely fluorescent kinetochore (no. 11), the functional centromere, and a less intensely fluorescent kinetochore (no. 9), the inactive centromere. The data suggest that in the process of tandem fusion (telomere-telomere between 11p and 9p), the centromere of chromosome 9 was not deleted, but, rather, inactivated.
D000906:Antibodies; D002460:Cell Line; D002503:Centromere / Q000276:immunology / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007621:Karyotyping; D008677:Metaphase
D000906:Antibodies; D007106:Immune Sera