Characterization of a tyrosine sulfotransferase in rat brain using cholecystokinin derivatives as acceptors.
An apparently novel tyrosyl sulfotransferase activity was detected in a crude microsomal fraction from rat cerebral cortex by using 3'-phosphoadenosine 5'-phospho[35S]sulfate [( 35S]PAPS) as the sulfate donor and various cholecystokinin (CCK) fragments or derivatives as acceptors. Among the latter, the shortest substrate was tert-butoxycarbonylaspartyltyrosine (Boc-Asp-Tyr), but the reaction was optimized by increasing the length of the peptide sequence on the C-terminal side up to tert-butoxycarbonylcholecystokinin octapeptide (Boc-CCK-8) as well as by the presence of acidic amino acid residues at the N-terminal side. Peptides with an N-terminal Tyr residue (e.g., CCK-7 or enkephalins) were not sulfated. With Boc-CCK-8 the optimum pH was 5.8, and apparent KM values were 0.14 +/- 0.02 mM for the peptide (0.5 microM PAPS) and 0.12 +/- 0.01 microM for PAPS (0.25 mM Boc-CCK-8). In the presence of 0.2 mM MnCl2 the Vmax of the reaction was enhanced without change of apparent affinities of the two substrates. The possible role of this sulfotransferase activity in posttranslational modification of CCK and other secretory proteins is suggested.
D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D002766:Cholecystokinin / Q000031:analogs & derivatives* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D011499:Protein Processing, Post-Translational; D051381:Rats; D013379:Substrate Specificity; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D002766:Cholecystokinin; D013466:Sulfurtransferases; D015238:Sulfotransferases; C039079:protein-tyrosine sulfotransferase