Membrane protein from rabbit T-lymphocytes, specifically binding staphylococcal enterotoxin A (SEA).
The presence of specific binding of SEA with membranes of lymphocytes from rabbit thymus is established. Components of a glycolipid nature are absent in the composition of the receptor complex for SEA on T-lymphocytes. Suitable conditions for the solubilization of the receptor membrane fraction by Triton X-100 are described. The SEA-binding membrane fraction is isolated by means of an affinity-chromatography method. The main component of the fraction is a protein with molecular mass 42 kd. The isolated protein inhibits the specific binding of [125I] SEA on cell (T-lymphocytes) and subcell (membrane) levels.
D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D004768:Enterotoxins / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D011817:Rabbits; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000378:metabolism
D004768:Enterotoxins; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; C026066:enterotoxin A, Staphylococcal