[Use of nucleic acid preparative electrophoresis in molecular oncological research].
Application of preparative electrophoresis in agarose gel for isolation of chromosomal and extrachromosomal genetic elements is described. The above-mentioned method is used for fractionation of chromosomal DNA according to the molecular weight; for isolation of the plasmid containing the insertion of the viral oncogene myc and for separation of the viral oncogene myc insertion from the vector (a linear form of plasmid pBR 322). The method can be successfully applied in molecular biology, microbiology and medicine.
D002877:Chromosomes, Human / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004926:Escherichia coli; D005814:Genes, Viral; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis; D008970:Molecular Weight; D009857:Oncogenes; D010957:Plasmids
D004269:DNA, Bacterial; D004247:DNA
Ispol'zovanie preparativnogo élektroforeza nukleinovykh kislot v molekuliarno-onkologicheskikh issledovaniiakh.