title: Swelling of the brain in mice caused by pertussis vaccine--its quantitative determination and the responsible factors in the vaccine.
abstract: Intracerebral injection of vaccine into the mouse induced swelling of the brain. The swelling reached the maximum in the intensity by day 1 and persisted for several days. A method for quantitative determination of the brain-swelling activity of the vaccine was developed. A positive regression coefficient was found only between the brain-swelling and the lymphocytosis-promoting activities. Such activity was no longer shown with the vaccine heat-treated for 30 min at 80 C, but it was restored upon addition of the lymphocytosis-promoting factor (LPF) that caused no brain swelling by itself. The activity, therefore, was ascribed to cooperation of LPF and a certain heat-stable component other than endotoxin contained by pertussis vaccine.
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000473:pathology*; D002552:Cerebral Ventricles / Q000187:drug effects / Q000473:pathology*; D004168:Diphtheria Toxoid / Q000633:toxicity*; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations / Q000633:toxicity; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000633:toxicity*; D013745:Tetanus Toxoid / Q000633:toxicity*
chemical_list: D004168:Diphtheria Toxoid; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004338:Drug Combinations; D010567:Pertussis Vaccine; D013745:Tetanus Toxoid