Cloning and chromosomal location of the alpha- and beta-globin genes from a marsupial.
The cDNA sequences encoding the adult alpha- and beta-globin polypeptides of a marsupial, the native cat Dasyurus viverrinus, have been cloned and their nucleotide sequences have been determined. Using these cDNA clones we have determined the chromosomal location of the native cat alpha- and beta-globin genes by in situ hybridization to fixed metaphase chromosomes and by hybridization to DNA extracted from chromosomes purified by a fluorescence-activated cell sorter. Using two independent methods of gene assignment we have shown the alpha- and beta-globin-like gene sequences to be asyntenic in the native cat, the alpha-globin-like sequences being on chromosome 2 and the beta-globin-like sequences being on chromosome 4.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D003001:Cloning, Molecular; D005434:Flow Cytometry; D005796:Genes; D005914:Globins / Q000235:genetics*; D008394:Marsupialia / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012156:Reticulocytes
D005914:Globins