Effect of acute and chronic ethanol treatment on renal prostaglandins in the rat.
To study the effects of ethanol on renal prostaglandins (PGs), we gave rats a dose of 3.0 g/kg body weight either as a single IP injection (acute treatment) or daily IP injections of the same dose for 23 consecutive days (chronic treatment). The availability of PG precursors was evaluated from the post mortem accumulation of PGF2 alpha in kidney as determined by gas chromatography-mass spectrometry, and the renal excretion of PGF2 alpha was measured by radioimmunoassay. Acute ethanol treatment had no dramatic effect on either tissue levels or urinary excretion rate of PGF2 alpha but chronic ethanol treatment significantly lowered renal PGF2 alpha levels. In another experiment we employed liquid ethanol diets and a pair feeding technique for 12 days to achieve tolerance and physical dependence on ethanol. The levels of PGF2 alpha and 6-keto PGF1 alpha were measured in kidney in rats killed at 2, 6, and 12 days post-treatment. The kidney levels of both prostaglandins were lowered throughout the period of chronic ethanol exposure but returned to normal within five days after withdrawal. Thus, chronic but not acute ethanol treatment leads to a depletion of the renal stores of prostaglandin precursors in the rat.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D015237:Dinoprost; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000378:metabolism* / Q000652:urine; D011460:Prostaglandins F / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D011453:Prostaglandins; D011460:Prostaglandins F; D000431:Ethanol; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost