Effects of female hormones on the activity of 3'-phosphoadenylylsulphate: desulphated heparan sulphate sulphotransferase in the endometrium of rabbit uterus.
Sulphotransferase activity in the microsomal fraction of the uterine endometrium of rabbits was measured with heparan sulphate, N-desulphated heparan sulphate and N-, O-desulphated heparan sulphate as exogenous substrates in the presence of 3'-phosphoadenylyl[35S]sulphate. Of the three acceptors, N-, O-desulphated heparan sulphate was most effectively radiolabelled. When N-, O-desulphated heparan sulphate was used as an acceptor, it was found that estrogen enhanced the sulphotransferase activity and that progesterone suppressed the effect of estrogen.
D000818:Animals; D002850:Chromatography, Gel; D004588:Electrophoresis, Cellulose Acetate; D004717:Endometrium / Q000201:enzymology*; D004967:Estrogens / Q000502:physiology*; D005260:Female; D010052:Ovariectomy; D011817:Rabbits; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism*; D013552:Swine
D004967:Estrogens; D013466:Sulfurtransferases; D015238:Sulfotransferases; C021350:heparitin sulfotransferase