Effect of forskolin on prostaglandin productions in isolated dog renal arteries.
Forskolin (1 to 100 microM), a direct activator of adenylate cyclase, did not have any effect on prostaglandin E2 and I2 productions in isolated dog renal arteries. However, forskolin at the lower concentrations (10 and 100 nM) markedly stimulated only prostaglandin E2 production. 8-Bromo-cyclic AMP (0.5 and 1 mM) failed to stimulate prostaglandin E2 and I2 productions. The results suggest that 1) forskolin stimulates only prostaglandin E2 production, not through the activation of adenylate cyclase and 2) the prostaglandin production system may be independent of the cyclic AMP-generating system in isolated dog renal arteries.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D015232:Dinoprostone; D004285:Dogs; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000378:metabolism; D012077:Renal Artery / Q000378:metabolism*
D011453:Prostaglandins; D011458:Prostaglandins E; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D015121:6-Ketoprostaglandin F1 alpha; D000262:Adenylyl Cyclases; D015232:Dinoprostone