Electrophoretic desorption of intact virus from immunoadsorbent.
A technique and apparatus are described for obtaining electrophoretic elution of virus from immunoadsorbent matrices. The method was applied to a well-known and easily purifiable virus, carnation mottle virus (CarMV). The technique was applicable even when the starting material was diluted and the virus recovered was indistinguishable from the original. Our aim was to avoid more drastic procedures that could damage virus structure (e.g. chaotropic chemicals, detergents, acidic pH's) and develop the mildest procedure possible. The apparatus is easy to work with and the procedure is simple and rapid.
D000818:Animals; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D007163:Immunosorbent Techniques; D011817:Rabbits; D014780:Viruses / Q000276:immunology / Q000302:isolation & purification*