Immunohistochemical distribution of the histone H1(0)/H5 variant in various tissues of adult Xenopus laevis.
The cellular distribution of the histone H1(0)/H5 variant has been examined immunohistochemically in various tissues of adult Xenopus laevis, using monoclonal antibodies against this variant that was isolated from erythrocyte nuclei. The H1(0)/H5 variant appears not to be erythrocyte-specific and appears to be present in all cell types of liver, stomach, and skin. In contrast, in oocyte nuclei the H1(0)/H5 variant cannot be detected, whereas they do contain H1; the nuclei of spermatogenic cells contain the H1(0)/H5 variant, but probably less than the somatic cells. In Xenopus no H1(0) variant distinct from H5 seems to occur and the H1(0)/H5 variant apparently may perform a functional role related to mammalian H1(0).
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000032:analysis*; D008099:Liver / Q000166:cytology; D009865:Oocytes / Q000166:cytology; D012867:Skin / Q000166:cytology; D013270:Stomach / Q000166:cytology; D014018:Tissue Distribution; D014982:Xenopus laevis / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D006657:Histones