Immunohistochemical detection of monocytes by the antiserum specific to monocytic esterase.
An antiserum specific to esterase Ib was produced in a rabbit. The antigen-antibody reaction was visualized by the strong esterase activity in the precipitin band in Ouchterlony double diffusion and immunoelectrophoresis. Immunohistochemical procedure demonstrated strong staining in the monocyte-infiltrated splenic sections and tissue sections of true histiocytic lymphoma. Negative results were observed in T- and B-cell lymphomas, granulocytic sarcoma, and chronic granulocytic leukemia. This antibody may be useful for the identification of monocytes and histocytes in paraffin-embedded tissue sections.
D000818:Animals; D000918:Antibody Specificity; D004950:Esterases / Q000276:immunology*; D006644:Histiocytes / Q000473:pathology; D006651:Histocytochemistry / Q000379:methods*; D007124:Immunoenzyme Techniques; D007951:Leukemia, Myeloid / Q000473:pathology; D008223:Lymphoma / Q000473:pathology; D009000:Monocytes / Q000201:enzymology* / Q000473:pathology; D011817:Rabbits
D004950:Esterases