Embryonic chicken skeletal, cardiac, and smooth muscles express a common embryo-specific myosin light chain.
It has been demonstrated that embryonic chicken gizzard smooth muscle contains a unique embryonic myosin light chain of 23,000 mol wt, called L23 (Katoh, N., and S. Kubo, 1978, Biochem. Biophys. Acta, 535:401-411; Takano-Ohmuro, H., T. Obinata, T. Mikawa, and T. Masaki, 1983, J. Biochem. (Tokyo), 93:903-908). When we examined myosins in developing chicken ventricular and pectoralis muscles by two-dimensional gel electrophoresis, the myosin light chain (Le) that completely comigrates with L23 was detected in both striated muscles at early developmental stages. Two monoclonal antibodies, MT-53f and MT-185d, were applied to characterize the embryonic light chain Le of striated muscles. Both monoclonal antibodies were raised to fast skeletal muscle myosin light chains; the former antibody is specific to fast muscle myosin light chains 1 and 3, whereas the latter recognizes not only fast muscle myosin light chains but also the embryonic smooth muscle light chain L23. The immunoblots combined with both one- and two-dimensional gel electrophoresis showed that Le reacts with MT-185d but not with MT-53f. These results strongly indicate that Le is identical to L23 and that embryonic chicken skeletal, cardiac, and smooth muscles express a common embryo-specific myosin light chain.
D000818:Animals; D000911:Antibodies, Monoclonal; D002642:Chick Embryo; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006321:Heart / Q000196:embryology; D007158:Immunologic Techniques; D008970:Molecular Weight; D024510:Muscle Development; D009130:Muscle, Smooth / Q000032:analysis* / Q000196:embryology; D009132:Muscles / Q000032:analysis* / Q000196:embryology; D009206:Myocardium / Q000032:analysis*; D009218:Myosins / Q000032:analysis*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D003101:Collodion; D009218:Myosins