Modification of DNA by glucose 6-phosphate induces DNA rearrangements in an Escherichia coli plasmid.
Reducing sugars such as glucose or glucose 6-phosphate (Glc-6-P) have been shown previously to modify the amino groups of nucleotides and single-stranded DNA. We have examined the mutagenic effect of Glc-6-P-induced lesions in the double-stranded DNA plasmid pBR322. Seventeen mutants of the Ampr Tets phenotype were isolated from plasmid preparations whose transforming capacity had been decreased by incubation with Glc-6-P. A number of the mutant plasmids were found to have undergone gross DNA alterations, including insertions and deletions, as well as the development of multiple species originating from a single cell. The ability of an endogenous reducing sugar to induce extensive DNA rearrangements suggests that these lesions may be significant contributors to cellular mutation.
D002872:Chromosome Deletion; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism*; D005669:Fusaric Acid / Q000494:pharmacology; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism*; D009154:Mutation / Q000187:drug effects*; D010084:Oxidation-Reduction; D010403:Penicillin Resistance; D010957:Plasmids; D013752:Tetracycline / Q000494:pharmacology; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; D013752:Tetracycline; D005669:Fusaric Acid