Isolation of cDNAs encoding human and gibbon GM-CSF.
We have used a mammalian cell expression cloning system to identify cDNA clones encoding human granulocyte-macrophage colony stimulating factor. The human clone was used as a hybridization probe to identify the corresponding sequence from a cDNA library prepared from a gibbon T-cell line. The human cDNA has been used to produce recombinant GM-CSF in monkey COS-1 cells. The purified protein from COS cells is very similar to the GM-CSF isolated from a continuous human T-cell line.
D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA / Q000302:isolation & purification*; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008264:Macrophages / Q000166:cytology*; D014162:Transfection
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D004247:DNA