Lysine decarboxylase assay by the pH-stat method.
A simple assay of lysine decarboxylase is described. It is based on measuring the rate of titration of OH- ions, released during decarboxylation with the aid of a pH-stat apparatus. The continuous recording of the reaction progress may be easily performed. Using the pH-stat method the Km for lysine decarboxylase from E. coli is 2.0 mM.
D002262:Carboxy-Lyases / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007201:Indicator Dilution Techniques
D002262:Carboxy-Lyases; C021520:lysine decarboxylase