Colony-stimulating activity from the new metastatic TS/A cell line and its high- and low-metastatic clonal derivatives.
We investigated the presence of colony-stimulating factor (CSF) in supernatants obtained from TS/A, a new metastatic murine cell line, and from its high-and low-metastatic clonal derivatives (E and F clones, respectively). TS/A cells produced a CSF in vitro that induced proliferation and differentiation of murine monocytic and granulocytic progenitors in agar cultures. In TS/A-bearing mice remarkable splenomegaly, blood granulocytosis and thymus depletion were observed along with a stimulatory activity in serum and a strong proliferative activity both in spleen and in bone marrow populations. Conditioned media from E clones showed an in vitro colony-stimulating activity greater than those of F clones. Mice injected subcutaneously with cells of all clones studied showed granulocytosis, splenomegaly and thymus depletion, although to varying degrees. However, no direct correlation between granulocytosis-splenomegaly and the number of spontaneous lung metastases was observed.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000032:analysis*; D005260:Female; D006098:Granulocytes; D007958:Leukocyte Count; D008264:Macrophages; D008325:Mammary Neoplasms, Experimental / Q000032:analysis* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D013936:Thymidine / Q000031:analogs & derivatives / Q000378:metabolism
D003115:Colony-Stimulating Factors; C012439:methylthymidine; D013936:Thymidine