Electrophoretic analysis of HLA-DR2 molecules isolated from HLA-Dw2 and HLA-Dw12 cell lines.
To answer the question of whether or not polymorphism exists among HLA-DR2 molecules derived from cells homozygous for HLA-DR2, but expressing different HLA-D specificities, HLA-DR2 molecules were isolated from HLA-Dw2 and HLA-Dw12 homozygous cells using a monoclonal antibody operationally monospecific for HLA-DR2, and were compared to each other by two-dimensional gel electrophoresis. No electrophoretically discernible polymorphism was found in either the heavy or the light chain subunits of the HLA-DR2 molecules. These findings are in marked contrast with previous observations that each of the HLA-DR4-associated HLA-D clusters expresses an electrophoretically distinct HLA-DR4 light chain.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D004586:Electrophoresis; D006681:HLA-D Antigens; D015802:HLA-DR2 Antigen; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D015802:HLA-DR2 Antigen; C039901:HLA-Dw12 antigens; D000949:Histocompatibility Antigens Class II