Determination of theophylline in serum with the Seralyzer Aris reagent strip test evaluated.
We evaluated a new Seralyzer Aris reagent strip test (Ames Div., Miles Labs.) for the determination of theophylline in human serum. The method is based on the monoclonal enzyme immunoassay with dry reagent chemistry. The analysis is rapid and simple to perform: results are available only 5-10 min after receipt of the sample. Intra-assay precision (CV) was 2.2-3.3% (n = 15) for theophylline concentrations of 5-25 mg/L; interassay CV was 5.9% (n = 19) at 15 mg/L. The results (y) agreed well with those by liquid chromatography (x): r = 0.949 (p less than 0.001), and y = 0.967x + 0.214. We conclude the method is useful for rapid evaluation of theophylline concentrations in asthmatic patients.
D000911:Antibodies, Monoclonal; D001249:Asthma / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D010783:Photometry / Q000295:instrumentation; D011934:Reagent Strips; D013806:Theophylline / Q000097:blood*
D000911:Antibodies, Monoclonal; D011934:Reagent Strips; D013806:Theophylline