A novel RNA digesting activity from commercial polynucleotide phosphorylase.
RNase A4 is a new RNase activity found as a contaminant in commercial polynucleotide phosphorylase. This enzyme has the ability of hydrolyzing the phosphodiester bond between pyrimidine-A in both loop and paired regions of RNA.
D002845:Chromatography; D004340:Drug Contamination; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D011117:Polyribonucleotide Nucleotidyltransferase / Q000032:analysis / Q000592:standards*; D012331:RNA, Fungal / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013379:Substrate Specificity
D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D011117:Polyribonucleotide Nucleotidyltransferase; D012260:Ribonucleases