Enhanced DNA synthesis of human glial cells exposed to human leukocyte products.
DNA synthesis was studied in primary glial cell cultures derived from adult human non-neoplastic and neoplastic brain tissues. Enhanced DNA synthesis occurred in 5/5 non-neoplastic astrocyte, one oligodendroglioma, and 2/5 astrocytoma cultures after exposure to medium containing 1.25-12.5% supernatant fluid (SF) from insoluble concanavalin A (Con A) stimulated unseparated or T lymphocyte-enriched human mononuclear leukocytes (MNL). Analyses of SF indicated that the presence of platelet-derived growth factor (PDGF) could not account for glial cell stimulation, and exposure to semi-purified interleukin-2 (IL-2) in amounts comparable to those in SF from Con A-stimulated MNL had no effect on glial cells. These data indicate that non-neoplastic astrocytes and other human glial cells are stimulated by products of human MNL.
D001254:Astrocytoma / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology; D007962:Leukocytes / Q000502:physiology*; D009457:Neuroglia / Q000378:metabolism*; D009837:Oligodendroglioma / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000502:physiology
D005904:Glial Fibrillary Acidic Protein; D007376:Interleukin-2; D010982:Platelet-Derived Growth Factor; D003208:Concanavalin A; D004247:DNA