Use of a metallochromic indicator to study intracellular calcium movements in skeletal muscle.
The transient increase in free myoplasmic calcium concentration due to depolarization of a skeletal muscle fibre is the net result of the release of calcium from the sarcoplasmic reticulum (SR) and its simultaneous removal by binding to various sites and by reuptake into the SR. We review here procedures recently developed in this laboratory for empirically characterizing the calcium removal processes in voltage-clamped fibres and for using such characterization to determine the time course of SR calcium release during a depolarizing pulse.
D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D009119:Muscle Contraction; D009132:Muscles / Q000378:metabolism* / Q000502:physiology; D009282:Naphthalenesulfonates; D011894:Rana pipiens; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D013816:Thermodynamics
D009282:Naphthalenesulfonates; C016238:antipyrylazo III; D002118:Calcium