title: Regulation of proliferation of bovine aortic endothelial cells, smooth muscle cells, and adventitial fibroblasts in collagen lattices.
abstract: We compared the proliferation of bovine aortic cells grown in collagen lattices. Smooth muscle cells continued to divide for 2 weeks while adventitial fibroblasts ceased to divide after 4-5 days. Endothelial cells did not proliferate within an untreated collagen lattice; however, if the lattice was covered with culture medium, endothelial cells populated its surface and proliferated to form a monolayer. We also found that both smooth muscle cells and endothelial cells, like fibroblasts, are able to contract a collagen lattice to a small fraction of its original volume, although endothelial cells are able to do so only if the lattice is covered with culture medium.
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology*; D002417:Cattle; D002452:Cell Count; D002455:Cell Division; D003094:Collagen; D003239:Connective Tissue Cells; D003470:Culture Media; D003584:Cytological Techniques; D004727:Endothelium / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D009130:Muscle, Smooth / Q000166:cytology*; D013936:Thymidine; D014316:Tritium
chemical_list: D003470:Culture Media; D014316:Tritium; D003094:Collagen; D013936:Thymidine