The formation of cerebrospinal fluid in two amphibians, Rana catesbeiana and Rana pipiens.
The cerebrospinal fluid (CSF) system of two amphibians, Rana catesbeiana and Rana pipiens has been perfused from the lateral ventricle to a site in the subarachnoid space close to the roof of the fourth ventricle. The CSF production rate (Vf) was measured by the dilution method using a perfusion fluid containing [14C]dextran in artificial CSF. For Rana catesbeiana Vf was 1.43 microliter min-1 or 0.2 microliter min-1 mg choroid plexus-1 and for Rana pipiens Vf was 0.2 microliter min-1 or 0.1 microliter min-1 mg choroid plexus-1. These control values declined by 35% and 14% respectively in the second half of the experimental period. Ouabain (5 X 10(-5) M) in the perfusion fluid for the second half of the experiment resulted in a much greater reduction in Vf, viz. 65% for Rana catesbeiana and 71% for Rana pipiens. Acetazolamide (1 X 10(-4) M) in the perfusion fluid for the second half of the experiment gave no change in Vf values when compared with control 74.5 +/- 4.8 microliters.
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D002555:Cerebrospinal Fluid / Q000502:physiology*; D002831:Choroid Plexus / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D011892:Rana catesbeiana; D011894:Rana pipiens; D013045:Species Specificity
D010042:Ouabain; D000086:Acetazolamide