title: Lack of correlation between hepatic microsomal progesterone 21-hydroxylase activity and the excretion of acidic metabolites in rabbit urine.
abstract: Hepatic microsomes were prepared from liver sections removed from anesthetised New Zealand White rabbits. Two groups of rabbits, distinguished by different levels of progesterone 21-hydroxylase, were compared for in vitro and in vivo metabolism of [3H]deoxycorticosterone/[14C]progesterone mixtures. In vitro, microsomes with low 21-hydroxylase activity gave significantly lower yields of pregnenoic acid than those with higher 21-hydroxylase activities. In vivo, these differences were not evident from an examination of the 3H/14C ratios of acidic urinary metabolites.
mesh_terms: D000818:Animals; D001711:Biotransformation; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D011374:Progesterone / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000652:urine*; D011817:Rabbits; D013255:Steroid 21-Hydroxylase / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*
chemical_list: C010952:4-pregnene-3,20-dione-21-oic acid; D011374:Progesterone; D013250:Steroid Hydroxylases; D013255:Steroid 21-Hydroxylase