A novel IgA protease from Clostridium sp. capable of cleaving IgA1 and IgA2 A2m(1) but not IgA2 A2m(2) allotype paraproteins.
Three bacterial strains of Bifidobacterium and Clostridium sp. from patients with inflammatory bowel disease (I.B.D.) and Streptococcus pneumoniae from a patient with pneumonia were identified to produce extracellular proteases cleaving IgA into Fab and Fc fragments. Although the proteases from the Bifidobacterium and the Streptococcus pneumoniae showed the characteristics of typical IgA1 proteases, cleaving the IgA of only the IgA1 subclass, the protease from Clostridium sp. revealed a dual substrate specificity, in that it cleaved both IgA1 and IgA2 of the A2m(1) allotype. The latter protease, however, did not show any activity with respect to the IgA2 of the A2m(2) allotype. Fc fragments isolated from the IgA1 and the IgA2 A2m(1) by digestion with the Clostridium sp. protease were identified to have an identical amino terminal residue of valine. The site of cleavage in both the alpha 1 and the alpha 2 of A2m(1) by the protease was assumed to be an identical peptide bond at Pro(221)-Val(222), which is a common one present just before the hinge of both the alpha 1 and the alpha 2 of the A2m(1) but not of the alpha 2 of the A2m(2). The protease was sensitive to ethylene-diamino tetraacetic acid, a chelating agent, similar to other already reported IgA1 proteases.
D001424:Bacterial Infections / Q000382:microbiology; D001644:Bifidobacterium / Q000201:enzymology* / Q000302:isolation & purification; D003013:Clostridium / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A; D007126:Immunoglobulin Allotypes; D010266:Paraproteins; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D012697:Serine Endopeptidases; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000302:isolation & purification; D013379:Substrate Specificity
D007070:Immunoglobulin A; D007126:Immunoglobulin Allotypes; D010266:Paraproteins; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase