High incidence of acute myeloid leukemia in SJL/J mice after X-irradiation and corticosteroids.
SJL/J mice which developed a high incidence of spontaneous reticulum cell neoplasms, developed a low rate incidence (20-25%) of myeloid leukemia (ML) after X-irradiation. The possible effect of adrenal steroid imbalance to radiation-induced ML in SJL/J mice was tested. Intact and thymectomized animals were exposed to a single dose of 300 r whole body irradiation and treated with either hydrocortisone acetate, prednisone, metyrapone and adrenocorticotropin as coleukemogenic agents. Hydrocortisone and prednisone exerted a marked coleukemogenic effect, increasing the ML incidence to a similar rate of about 50-70%, at a mean latent period of 300 days. Prominent leukemic infiltration were observed in the bone marrow, spleen, lymph nodes and liver of the leukemic animals. Results of cytological and histological studies, including cytochemistry and ultrastructure, were all consistent with the diagnosis of acute myeloid leukemia (AML). Since AML is the type of human secondary leukemia which appears increasingly in patients treated with alkylating drugs and/or irradiation and corticosteroids for Hodgkin's disease or other neoplastic diseases, the experimental model of AML induced in SJL/J mice could be used for elucidation of mechanisms of leukemogenesis in secondary leukemia.
D000305:Adrenal Cortex Hormones / Q000633:toxicity*; D000818:Animals; D005260:Female; D015470:Leukemia, Myeloid, Acute / Q000209:etiology* / Q000473:pathology; D007953:Leukemia, Radiation-Induced / Q000209:etiology* / Q000473:pathology; D008797:Metyrapone / Q000633:toxicity; D051379:Mice; D008815:Mice, Inbred Strains
D000305:Adrenal Cortex Hormones; D008797:Metyrapone