[Cultivation of reaggregated brain cells in rapidly rotating mini-rollers].
An original method of cultivation of reaggregated brain cells with the aid of high-speed portable mini-rollers is described. The mini-roller consists of parallel rollers and an electric motor rotating the flasks at a speed of 60 to 70 rpm. The Moscona technique was used for preparing brain cell suspensions. During cultivation of the suspension of dissociated cells in high-speed mini-rollers, reaggregates with an internal organotypic structure were obtained. The method suggested provides stable and reproducible results.
D000818:Animals; D001921:Brain / Q000166:cytology*; D002449:Cell Aggregation; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans
Kul'tivirovanie reagregirovannykh kletok mozga v bystro vrashchaiushchikhsia mini-rollerakh.