title: Characteristics of lipid peroxidative conduction block induced by an organic hydroperoxide in axons of isolated frog nerve.
abstract: The direct effects of lipid peroxidation on axonal conduction were investigated by application of tertiary-butyl hydroperoxide (t-BOOH) to the isolated common peroneal nerve of the frog (Rana catesbeiana). The powerful oxidizing agent t-BOOH caused a concentration-related (0.03-3.0%) block of action potential conduction. This effect, presumably due to axonal lipid peroxidation, was progressive, with the time required for the conduction impairment to occur also being a function of t-BOOH concentration. In contrast, tertiary butyl alcohol had no effect even at a 3.0% concentration. The gamma-fibers in the nerve were the most sensitive to t-BOOH conduction block, followed in order by the larger diameter beta-fibers and the even larger alpha-fibers. The rate of decrease in conduction was faster in nerves that were stimulated continuously (1 Hz) than in those that were activated only at specific measurement times, indicating an association between axonal depolarization and susceptibility to peroxidative conduction block. Recovery of conduction was observed particularly in alpha- and beta-fibers. The rate and extent of recovery were inversely proportional to the concentration of t-BOOH, suggesting that moderate peroxidative damage is potentially reversible. The possible relationship of these results to lipid peroxidative axonal damage in acute central nervous system injury is discussed.
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001369:Axons / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism* / Q000502:physiology; D009407:Nerve Block; D009431:Neural Conduction / Q000187:drug effects*; D010543:Peroneal Nerve / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010545:Peroxides / Q000494:pharmacology*; D011892:Rana catesbeiana; D051381:Rats; D011930:Reaction Time / Q000502:physiology; D020122:tert-Butylhydroperoxide
chemical_list: D008054:Lipid Peroxides; D010545:Peroxides; D020122:tert-Butylhydroperoxide