title: The effect of tunicamycin on transferrin synthesis and secretion in hormonally stimulated explants of rabbit mammary gland.
abstract: Pregnant rabbit mammary gland explants cultured with insulin, prolactin and cortisol synthesize and secrete transferrin radiolabelled with [3H]leucine or [3H]mannose. Addition of tunicamycin inhibits the glycosylation but not synthesis of the [3H]leucine-labelled polypeptide. Secretion of the non-glycosylated protein is only marginally inhibited.
mesh_terms: D000818:Animals; D005260:Female; D005944:Glucosamine / Q000031:analogs & derivatives*; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011817:Rabbits; D014168:Transferrin / Q000096:biosynthesis* / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D007328:Insulin; D014168:Transferrin; D014415:Tunicamycin; D011388:Prolactin; D005944:Glucosamine; D006854:Hydrocortisone