title: Primary tissue culture of spontaneously regressing flat warts. In vitro attack by mononuclear cells against wart-derived epidermal cells.
abstract: Although tumors may be resolved due to host immune response, it is difficult to obtain direct evidence of this in man. Numerous flat warts, human papilloma virus type 3-induced papillomas, disappear systemically and simultaneously after showing inflammatory changes. Histologically, there is a dense cellular infiltration composed of lymphocytes and mononuclear phagocytes as identified by alpha-naphthyl acetate esterase staining in situ, the former being predominant in most cases. The primary tissue culture of such inflamed flat warts from ten cases revealed a proliferation of wart-derived keratinocytes as is the case with ordinary flat warts. However, in nine of the ten cases, massive mononuclear cells, most of which were T-lymphocytes, migrated out of the explants and began to attack these keratinocytes, inducing degenerative changes. These findings indicate that cell-mediated tumor cell destruction rather than antiviral reaction induces systemic spontaneous regression of multiple papillomas in man.
mesh_terms: D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D001706:Biopsy; D046508:Culture Techniques; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D008297:Male; D009000:Monocytes / Q000276:immunology; D009365:Neoplasm Regression, Spontaneous; D010212:Papilloma / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal