title: Ca2+, Mg2+-dependent endonuclease activity in different subpopulations of spleen cells from normal and erythroleukemic mice.
abstract: We have detected Ca2+, Mg2+-dependent endonuclease activity in spleen cells of normal, Friend erythroleukemic, and phenylhydrazine-treated mice. When nuclei were isolated and incubated in the presence of Ca2+ and Mg2+ ions, the activity resulted in the production of 3'-OH termini in the cellular DNA and the release of chromatin due to internucleosomal DNA fragmentation. This enzyme activity was chromatin-bound and could be extracted from chromatin in an active form in 0.35 M KCl. The majority of endonuclease activity from erythroleukemic spleens was present in nuclei of precursor erythroid cells of low buoyant density (1.025-1.05 g/ml). Uninfected normal splenic tissue contained an endonuclease activity which was almost entirely confined to a B-lymphocyte population of high buoyant density (greater than 1.07 g/ml). Erythroid cell-enriched spleens from phenylhydrazine-treated mice exhibited a distribution of endonuclease activity in cells at low and high densities reflecting a mixture of erythroid and lymphoid cells. Cloned erythroleukemic cell lines propagated in vitro lacked cells of low density and showed no detectable endonuclease activity. However, nuclei from these cell lines were susceptible to exogenously added endonuclease extracted from erythroleukemic spleen cells. These same cell lines propagated as subcutaneous tumors contained endonuclease activity and a morphologically-similar low-density cell population which accounted for the endonuclease activity in these tumors. Nuclei from cloned lymphoid cell lines, representing different B-lymphocyte phenotypes, showed differences in the presence of endonuclease activity. Among the cell lines tested, only those expressing late B-cell markers showed detectable endonuclease activity.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D002843:Chromatin / Q000201:enzymology; D002999:Clone Cells; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology*; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology* / Q000473:pathology; D008214:Lymphocytes / Q000201:enzymology*; D051379:Mice; D008811:Mice, Inbred DBA; D010659:Phenylhydrazines / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D013601:T-Lymphocytes / Q000201:enzymology
chemical_list: D002843:Chromatin; D010659:Phenylhydrazines; C030299:phenylhydrazine; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease