Induction of ornithine decarboxylase in the liver and spleen of mice by interleukin 1-like factors produced from a macrophage cell line.
Injection of culture medium of P388D1 cells, a murine macrophage cell line, into mice produced a rapid induction of ornithine decarboxylase in the liver and spleen. The ornithine decarboxylase-inducing factor in the medium was purified by gel-filtration and chromatofocusing. With these procedures, the factor was usually found in the fraction of interleukin 1 (a lymphocyte-activating factor), indicating that the factor is identical with interleukin 1 or a molecule closely related to interleukin 1. The ornithine decarboxylase induction by the factor was suppressed by cycloheximide, but actinomycin D did not suppress the induction, or rather enhanced it. These findings may provide valuable information on the important roles of macrophages in immune responses or in inflammatory reactions.
D000818:Animals; D002460:Cell Line; D002845:Chromatography; D003470:Culture Media / Q000032:analysis; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005502:Food; D007375:Interleukin-1 / Q000302:isolation & purification / Q000502:physiology*; D008099:Liver / Q000201:enzymology*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D013154:Spleen / Q000201:enzymology*; D013217:Starvation / Q000201:enzymology
D003470:Culture Media; D007375:Interleukin-1; D003609:Dactinomycin; D003513:Cycloheximide; D009955:Ornithine Decarboxylase