title: Polyclonal B-cell activation of rabbit Peyer's patch.
abstract: This study delineates the culture conditions and cellular requirements for pokeweed mitogen (PWM)-induced polyclonal B-lymphocyte activation of rabbit Peyer's patch cells for immunoglobulin secretion. This system was optimal with a cell density of 3 X 10(5) cells/ml and a culture duration of 7 days, in round-bottom containers. Although glass-plate-adherent cells did not appear to influence this process, the immunoglobulin responses to PWM were T cell dependent. This study supplies a foundation for further work on the mechanisms of polyclonal activation of B cells in the rabbit Peyer's patch.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D010581:Peyer's Patches / Q000276:immunology*; D011817:Rabbits / Q000276:immunology*; D013997:Time Factors
chemical_list: D008934:Mitogens