Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.
Antibodies raised against the protein encoded by a lacZ-CDC28 in-frame fusion were shown to immunoprecipitate the CDC28 product from yeast cell lysates. The polypeptide p36CDC28 is a phosphoprotein of apparent Mr 36,000. Immune complexes prepared from yeast cell lysates by using anti-CDC28 antibody were found to possess a protein kinase activity, as determined by the transfer of label from [gamma-32P]ATP to a coprecipitated Mr 40,000 protein of unknown identity or function (p40). This activity was absent or thermolabile when extracts were prepared from several different cdc28 temperature-sensitive strains. The protein kinase activity was dependent on Zn2+ and transferred phosphate specifically to serine and threonine residues.
D000595:Amino Acid Sequence; D000936:Antigen-Antibody Complex; D002453:Cell Cycle; D005656:Fungal Proteins / Q000378:metabolism; D007526:Isoelectric Point; D008970:Molecular Weight; D009154:Mutation; D010750:Phosphoproteins / Q000502:physiology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics* / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013696:Temperature
D000936:Antigen-Antibody Complex; D005656:Fungal Proteins; D010750:Phosphoproteins; D011494:Protein Kinases