High-performance liquid affinity chromatography of beta-lactamase.
New silica and organic polymer-based, wide-pore, affinity chromatographic phases have been developed for beta-lactamase purification. The resins have been evaluated in terms of binding capacity, ligand coverage, protein recovery and long-term stability of activated resins prior to ligand attachment. The columns have been applied to the isolation of beta-lactamase from fermentation media. Protein purity following high-performance liquid affinity chromatography was assessed by polyacrylamide gel electrophoresis.
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D004355:Drug Stability; D005285:Fermentation; D007475:Ion Exchange Resins; D007700:Kinetics; D010405:Penicillinase / Q000302:isolation & purification; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D001618:beta-Lactamases / Q000302:isolation & purification*
D007475:Ion Exchange Resins; D011506:Proteins; D010405:Penicillinase; D001618:beta-Lactamases; D003488:Cyanogen Bromide