[Use of galascorbin as a fixative of histological material for light and electron microscopy].
A complex of sodium salts of gallic and ascorbic acids (galascorbin) is recommended as an additive to the fixative for improving the quality of histological material fixation. Its main advantage as compared to natural tannids is an easiness of penetration into the depth of tissues and, in comparison to other fixatives (aldehydes, alcohol), a more efficient fixation and retention of intra- and extracellular substances in tissues. Prescriptions of the galascorbic fixatives for light and electron microscopy are given. A method of staining of preparations specifically adapted for galascorbic fixation is suggested for light microscopy. Methods of both fixation and staining are intended for scientific purposes and for use by prosectors.
D000085:Acetates; D001205:Ascorbic Acid; D004338:Drug Combinations; D005419:Flavonoids; D005557:Formaldehyde; D006652:Histological Techniques; D047348:Hydrolyzable Tannins; D008853:Microscopy / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D013634:Tannins
D000085:Acetates; D004338:Drug Combinations; D005419:Flavonoids; D047348:Hydrolyzable Tannins; D013634:Tannins; D005557:Formaldehyde; C002944:galascorbin; D001205:Ascorbic Acid
Primenenie galaskorbina v kachestve fiksatora gistologicheskogo materiala dlia svetovoĭ i élektronnoĭ mikroskopii.