Generation of functional I-Ed variants from an antigen-presenting macrophage cell line.
We report the ability of the BAC1.2 macrophage cell line to present antigen both to populations of antigen-primed lymph node cells and to T cell hybridomas. We also describe the selection of I-Ed variants derived from this cell line. These variants have lost the ability to present antigen to an I-Ed plus ovalbumin-specific T cell line, but retain the ability to stimulate an I-Ad plus ovalbumin-restricted T cell line, and a T cell line reactive with I-Ed alone. These variants should aid in the elucidation of structural elements of the I-E molecule involved in the interaction with helper T cells.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D005434:Flow Cytometry; D014644:Genetic Variation; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D000949:Histocompatibility Antigens Class II; D006825:Hybridomas / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology
D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen