Pre-alpha 2-elastase inhibitor of the horse: a hybrid molecule between alpha 1-proteinase inhibitor and alpha 2-beta 1-glycoprotein.
Pre-alpha 2-elastase inhibitor of horse plasma has recently been isolated in our laboratory. In this article we demonstrate that the inhibitor is a composite structure built of alpha 1-proteinase inhibitor and alpha 1-beta 1-glycoprotein. The compound inhibitor is biologically active, although it has previously been shown that its enzyme specificity is different from that of free alpha 1-proteinase inhibitor. Our observations are based on immunochemical cross-reactions between pre-alpha 1-elastase inhibitor and antibodies to alpha 2-beta 1-glycoprotein as well as antibodies to alpha 1-proteinase inhibitor. Pre-alpha 2-elastase inhibitor could be dissociated into its parent components by dithiothreitol. After dissociation, the alpha 1-proteinase inhibitor portion retained its biological activity.
D000818:Animals; D000906:Antibodies; D001798:Blood Proteins / Q000276:immunology; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D004229:Dithiothreitol / Q000494:pharmacology; D004586:Electrophoresis; D005340:Fibrinogen; D006023:Glycoproteins / Q000276:immunology; D006736:Horses / Q000378:metabolism*; D007122:Immunoelectrophoresis; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000302:isolation & purification*; D000515:alpha 1-Antitrypsin
D000906:Antibodies; D001798:Blood Proteins; D006023:Glycoproteins; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D005340:Fibrinogen; D010196:Pancreatic Elastase; D004229:Dithiothreitol