Specific immunoassay of alpha-amylase isoenzymes in human serum.
A monoclonal antibody (66C7) was prepared that specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and the remaining (pancreatic) amylase activity is assayed. Results by the two methods correlate well.
D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000097:blood*; D008433:Mathematics; D010179:Pancreas / Q000201:enzymology; D012463:Saliva / Q000201:enzymology; D000516:alpha-Amylases / Q000097:blood*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D000516:alpha-Amylases