title: Different cellular requirements for inducing contact sensitivity and non-specific unresponsiveness with hapten-conjugated lymphoid cells.
abstract: Primary contact sensitivity (CS) to Ox and non-specific unresponsiveness to other unrelated delayed-type hypersensitivity (DTH) containing CS were induced simultaneously by i.v. administration of oxazolone(Ox)-conjugated spleen cells. The subpopulation of spleen cells which serves as carriers for the induction of primary CS consisted of plastic-adherent, Thy 1-negative macrophage-like cells, and the carriers for the induction of non-specific unresponsiveness were non-adherent, Thy 1-positive cells. Further, allogeneic spleen cells were not capable of acting as carriers for the induction of primary CS, though H-2 restriction was not observed in the induction of non-specific unresponsiveness by Ox-conjugated cells. Thus, these two phenomena were shown to be induced by distinct pathways or distinct mechanisms. Adult thymectomy or treatment with cyclophosphamide (CY) prevents the induction of non-specific unresponsiveness. Moreover, an interval of 7 days was required for almost complete suppression of CS between treatment with Ox-conjugated spleen cells and sensitization with picryl chloride (PCl). These results suggest that relatively short-lived, CY-sensitive T cells participate in the induction of the non-specific unresponsiveness induced with Ox-conjugated cells.
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology; D001202:Ascitic Fluid / Q000276:immunology; D002448:Cell Adhesion; D003520:Cyclophosphamide / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010081:Oxazolone / Q000276:immunology; D010853:Picryl Chloride; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013934:Thymectomy
chemical_list: D000961:Antilymphocyte Serum; D010081:Oxazolone; D003520:Cyclophosphamide; D010853:Picryl Chloride