High-performance liquid chromatographic identification of beta-lactam antibiotics produced by dermatophytes.
Thirteen of 48 dermatophyte isolates were found by bioassay to produce beta-lactam antibiotics and seven produced other antibiotics. Estimation and detection of specific beta-lactams in culture broths by derivatization and HPLC was only possible following concentration and extraction procedures. Analysis of the concentrated broths demonstrated the production of penicillin X and penicillin G by two Trichophyton mentagrophytes strains and by one Microsporum canis strain; one further T. mentagrophytes strain produced only penicillin X. Additions of the beta-lactam side-chain precursors, phenylacetic acid and phenoxyacetic acid, to fermentation media failed to increase the antibiotic titres.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification; D003883:Arthrodermataceae / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D010405:Penicillinase; D013045:Species Specificity; D014005:Tinea / Q000209:etiology; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D010405:Penicillinase