[Cultivation of bone marrow in a medium conditioned by lymphoid organ cells].
Conditioned media (CM) from short-term cultures of splenocytes or thymocytes stimulated by Con A inhibit the growth of fibroblast colonies and raise the survival of non-adherent cells (NAC) in primary monolayer cultures of mouse bone marrow. The double effect of the CM is dose-dependent. The self-renewal of NAC in CM cultures is effected during two weeks.
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D003584:Cytological Techniques; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects; D013997:Time Factors
D003470:Culture Media; D003208:Concanavalin A
Kul'tivirovanie kostnogo mozga v srede, konditsionirovannoĭ kletkami limfoidnykh organov.