Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I).
Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene has been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage lambda and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormone's synthesis were chosen in an SMC-lac z fusion assay. The amounts of SMC accumulated in E. coli were influenced by mutations at two chromosomal loci, lon and htpR.
D049069:ATP-Dependent Proteases; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D010450:Endopeptidases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics; D007334:Insulin-Like Growth Factor I; D009154:Mutation; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D049070:Protease La; D012697:Serine Endopeptidases; D013002:Somatomedins / Q000235:genetics*
D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D010450:Endopeptidases; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La