title: Leishmania tropica: differences in the antigenicity of promastigotes and amastigotes.
abstract: In vitro bioassays were used to compare T-cell responses induced by the intramacrophage amastigote stage and the sandfly-borne promastigote stage of Leishmania tropica. Lymph node cells from mice immunized with sonicated promastigotes or amastigotes incorporated in Freund's complete adjuvant were assayed for their ability to proliferate and to release interleukin 2 following in vitro challenge with promastigote or amastigote antigen. The levels of the proliferative and interleukin 2 synthetic responses of cells from promastigote and amastigote immunized mice were quite distinct. Cells from mice immunized with promastigotes demonstrated a vigorous in vitro response to the homologous antigen, but a reduced response to the potentially cross-reactive amastigotes. In contrast, cells from mice immunized with amastigotes mounted a weak response to the homologous antigen, but a consistently greater response to promastigote antigen. This unusual response was similar when 8 M urea extracts were used as immunogens and test antigens. In general, interleukin 2 production by immune mice paralleled the results from the T-cell proliferation assays. These results are discussed in relation to evasion of host immunologic detection by the intramacrophage amastigote stage.
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D007700:Kinetics; D007891:Leishmania / Q000254:growth & development / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology / Q000469:parasitology; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D007376:Interleukin-2