Induction of interleukin 2 from a murine B cell tumor by a factor found in immune serum.
The murine B cell tumor line 2 PK-3 secretes T cell growth factor activity after incubation for 6 to 48 hr with a factor present in heterologous immune serum. T cell growth factor derived from 2 PK-3 was compared with IL 2 produced by the Con A-induced T lymphoma cell line EL-4 G12. These studies indicated that T cell growth factor activities derived from both cell lines were similar with respect to m.w., pI values, and the ability to support growth of two IL 2-dependent T cell clones. Three preparations of immune sera were found to be active in the induction of IL 2 activity from 2 PK-3 cells, including rabbit anti-mouse brain, rabbit anti-complete Freund's adjuvant, and goat anti-mouse Ig. None of these preparations, however, induced IL 2 from EL-4 G12 cells. It was also observed that LPS synergized with immune serum to produce enhanced activity. Normal sera prepared from unimmunized animals were not active in the induction of IL 2 activity. Fractionation of immune serum on protein A Sepharose suggested that the IL 2-inducing agent is not IgG.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D001921:Brain / Q000276:immunology; D002460:Cell Line; D002846:Chromatography, Affinity; D002999:Clone Cells / Q000378:metabolism; D004586:Electrophoresis; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D007106:Immune Sera / Q000032:analysis / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000302:isolation & purification; D008223:Lymphoma / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011817:Rabbits; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D007376:Interleukin-2; D005620:Freund's Adjuvant