title: Phenotyping of proliferating cells in cultures of human lymphocytes.
abstract: Lymphocytes stimulated with mitogens, alloantigens or soluble antigens were pulsed with tritiated thymidine to label proliferating cells. Aliquots of the cells were labeled with OKT 4 or OKT 8 mouse monoclonal antibodies and then incubated in microtiter wells coated with goat anti-mouse immunoglobulin G (IgG). Unattached cells were flushed out of the wells: individual wells were then separated for counting in scintillation fluid in a B counter. This method permits simple characterization of the relative T cell subset proliferative responses and is clinically applicable.
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division; D006801:Humans; D008213:Lymphocyte Activation; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface