Cytotoxicity of adherent mononuclear cells detached after 24 hours of culture.
During the first day of culture some of the initially adherent mononuclear cells detach from the monolayers. On the initial day of culture these adherent cells are cytolytic. However, as the cells are cultured and the detached cells are removed, there is a disappearance of the strong cytolytic activity seen with the freshly isolated adherent monocytes. In contrast, it was found that the detached cells continued to exhibit high cytolytic capabilities. These detached cells were further characterized and found to consist of 30% T lymphocytes, 22% B lymphocytes, 35% monocytes, and 13% dendritic cells. Following sheep red blood cell (RBC) rosetting, the cytotoxic cells were found in the non-rosetting fraction and thus were non-T lymphocytes.
D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002448:Cell Adhesion; D002469:Cell Separation; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008221:Lymphoid Tissue / Q000166:cytology; D009000:Monocytes / Q000145:classification / Q000276:immunology* / Q000502:physiology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013997:Time Factors