Enhanced thromboxane synthesis and vacuolization in human polymorphonuclear leucocytes induced by human lymphokine containing supernatants.
PMN's were cultivated in vitro and treated with supernatants obtained from mitogenic-induced lymphocytes of human tonsil. Cytoplasmic vacuolization increased with time and there was a lower number of neutrophilic clumps in the culture treated with a supernatant containing lymphotoxin. In addition, the PMN's released more thromboxane B2 which was inhibited by indomethacin. We conclude that the action of lymphotoxin on the target PMN's is not mediated by the production of thromboxane B2.
D000328:Adult; D000818:Animals; D002417:Cattle; D002648:Child; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects; D008222:Lymphokines / Q000494:pharmacology*; D008233:Lymphotoxin-alpha / Q000378:metabolism; D051379:Mice; D009504:Neutrophils / Q000378:metabolism*; D009940:Organoids / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013268:Stimulation, Chemical; D013929:Thromboxane B2 / Q000096:biosynthesis*; D013997:Time Factors; D014617:Vacuoles / Q000378:metabolism*
D008222:Lymphokines; D008233:Lymphotoxin-alpha; D010835:Phytohemagglutinins; D013929:Thromboxane B2; D007213:Indomethacin