title: Identification of B cells in multilineage hematopoietic colonies derived from cells of patients with lymphocytic lymphoma.
abstract: Pluripotent stem cells from human bone marrow can be identified in culture by their ability to form multilineage hematopoietic colonies containing different myeloid lineages and T cells of different phenotypes. The observation of a common progenitor of myeloid and lymphoid cells in normal and disturbed hematopoiesis prompted the question of whether B cells are part of the differentiation program of stem cells. The availability of hybridomas of azaguanine-resistant T-cell lines secreting monoclonal growth factors for B cells and clinical conditions that are considered to originate from malignant B cells might facilitate this investigation. We were able to identify surface immunoglobulin and B-cell-associated antigen-positive cells within such colonies, indicating that B cells are generated from a myelolymphopoietic stem cell. This report describes the presence of B cells in these colonies derived from bone marrow cells of patients with non-Hodgkin lymphoma.
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D001854:Bone Marrow Cells; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007072:Immunoglobulin D / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008223:Lymphoma / Q000097:blood*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
chemical_list: D007072:Immunoglobulin D; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell