The proliferative effects of human GM-CSF alpha and beta and murine G-CSF in microwell cultures of fractionated human marrow cells.
Blast cell-enriched and promyelocyte-myelocyte-enriched fractions of human marrow were prepared by fluorescence-activated cell sorting using an antineutrophil monoclonal antiserum. Cells from both fractions proliferated in microwell cultures when stimulated by placental or bladder cancer cell conditioned medium containing colony stimulating factor. Semipurified preparations of both GM-CSF alpha and beta from bladder cancer cell conditioned medium were effective proliferative stimuli for both cell populations. Pure murine G-CSF, GM-CSF, M-CSF and Multi-CSF failed to stimulate detectable proliferation in blast cell fractions containing granulocyte-monocyte progenitors. However, G-CSF was an effective proliferative stimulus for human promyelocytes and myelocytes leading to the formation of differentiating granulocytic progeny. G-CSF also stimulated the proliferation of human promyelocytic leukemic cells. Promyelocyte-myelocyte-enriched fractions of human marrow appear to be useful target cells for monitoring the proliferative effects of human-active CSFs in microwell cultures.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003470:Culture Media; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid / Q000473:pathology; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D001749:Urinary Bladder Neoplasms / Q000378:metabolism
D003115:Colony-Stimulating Factors; D003470:Culture Media