Cell-free translation of the vitamin K-dependent bone protein osteocalcin.
The primary gene product of the vitamin K-dependent bone matrix protein, osteocalcin, has been identified by immunoprecipitation of cell-free translated proteins from 4 week rat calvariae mRNA preparations. Peptides of 9.8kd and 12kd, precipitated with a polyclonal affinity selected species specific antibody raised to purified rat osteocalcin, accounted for 1-2% of labelled proteins and were displaced by rat osteocalcin. These studies demonstrate that the 5800 molecular weight osteocalcin is synthesized as a precursor of approximately twice its size. The size of the propeptide, with a molecular weight of 4.3kd, is consistent with other known secreted vitamin K-dependent blood proteins.
D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D015675:Osteocalcin; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014812:Vitamin K / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D015675:Osteocalcin; D014812:Vitamin K