title: Macrophage chemotaxis in experimental tooth movement in the rat.
abstract: Extracts from tissue surrounding teeth being moved contained chemotactic activity for peritoneal exudate cells (PEC). The tissue was homogenized, extracted and diafiltered using a 100,000 mol. wt cut-off filter. The filtrate contained statistically-significant chemotactic activity, whereas the retentate did not. Further fractionation of the filtrate using an isoelectric focusing column produced a filtrate fraction in the pH range between 5.0 and 6.8 which was chemotactically active. This sample was analysed for purity and mol. wt using sodium dodecylsulphate polyacrylamide-gradient gel electrophoresis (SDS-PAGE). Gels of the active fraction stained with Coomassie blue showed two distinct bands with molecular weights of 69,000 and 60,000.
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000473:pathology; D002630:Chemotactic Factors / Q000302:isolation & purification; D002633:Chemotaxis; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000276:immunology*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D014086:Tooth Mobility / Q000276:immunology*
chemical_list: D002630:Chemotactic Factors