title: Point mutations that affect translation initiation in the Escherichia coli gal E gene.
abstract: This paper describes the selection and characterization of several mutations in the Escherichia coli galactose operon that affect translation initiation of the galE gene but are located outside of the Shine-Dalgarno sequence and the initiator codon. One mutation lies in the gal promoter region and shifts transcription initiation from the galP1 to the galP2 promoter. This results in a gal messenger that is five nucleotides longer and that is translated threefold more efficiently in vivo. This accords with previous observations from in vitro experiments which showed that the longer gal messenger was better translated (Queen & Rosenberg, 1981). The other mutations that affect galE translation are located in the coding sequence immediately downstream from the initiator codon. In contrast to the promoter mutation, these cause alterations in galE expression only when the gene carries a mutated initiator codon or Shine-Dalgarno sequence and have no effect on the wild-type galE gene. These findings are discussed with respect to our present knowledge of translation initiation mechanisms.
mesh_terms: D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005690:Galactose / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D009876:Operon; D014176:Protein Biosynthesis
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D005690:Galactose