Immunocytochemical detection of acetylcholine in the rat central nervous system.
A specific antibody to acetylcholine was raised and used as a marker for cholinergic neurons in the rat central nervous system. The acetylcholine conjugate was obtained by a two-step immunogen synthesis procedure. An enzyme-linked immunosorbent assay was used to test the specificity and affinity of the antibody in vitro; the results indicated high affinity. A chemical perfusion mixture of allyl alcohol and glutaraldehyde was used to fix the acetylcholine in the nervous tissue. Peroxidase-antiperoxidase immunocytochemistry showed many acetylcholine-immunoreactive cells and fibers in sections from the medial septum region.
D000109:Acetylcholine / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping; D002799:Cholinergic Fibers / Q000033:anatomy & histology*; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats
D000109:Acetylcholine