Antibodies to granulocytes detected by an indirect immunofluorescence method not requiring chemical modification of cells.
IgG antibodies to neutrophil polymorphonuclear cells (PMNs) were detected by a simple indirect immunofluorescence method that did not require chemical or enzymatic modification of the cells. Based on recent information about the human PMN Fc gamma-receptor, nonspecific binding of IgG to PMNs was virtually completely prevented by incubating cells at 37 degrees C with human serum diluted 1 to 3 in the presence of a high concentration of rabbit IgG. This also facilitated distinction of sera with weak anti-PMN activity from normal sera. On the other hand, nonspecific staining remained on a fraction (10-25%) of mononuclear leukocytes. Following first-time transfusion of 0.5 to 5 units of whole blood or red cell concentrate, 4 of 27 patients (15%) developed anti-leukocyte antibodies. Anti-leukocyte antibodies were detected in 51 percent of 47 patients with reported febrile transfusion reactions; the IgG antibodies bound to PMNs in 38 percent of these 47 patients. Since only about one in three patients with febrile transfusion reactions had detectable IgG anti-PMN antibodies, fever may be caused by lysis of leukocytes other than PMNs.
D000017:ABO Blood-Group System / Q000276:immunology; D001666:Binding Sites, Antibody; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D006680:HLA Antigens / Q000276:immunology; D015789:HLA-A2 Antigen; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D009504:Neutrophils / Q000276:immunology*; D011108:Polymers; D065227:Transfusion Reaction
D000017:ABO Blood-Group System; D006680:HLA Antigens; D015789:HLA-A2 Antigen; D007518:Isoantibodies; D011108:Polymers; D005557:Formaldehyde; C003043:paraform