The modification of HLA-B27-positive lymphocytes by the culture filtrate of Klebsiella K43 BTS 1 is a metabolically active process.
The culture filtrate of some bacteria contains a modifying factor which specifically interacts with the cells of HLA-B27-positive normal individuals (BS+ AS-); this modification results in a serologically detectable change similar to that seen on the cells of patients with ankylosing spondylitis (B27+ AS+). Puromycin, 5-azacytidine, actinomycin D, cyclosporin A, dexamethasone, indomethacin, colchicine, quinacrine, chlorpromazine but not tunicamycin can effectively inhibit the in vitro modification of HLA-B27-positive lymphocytes from normal individuals. These studies suggest that a number of different metabolic pathways may be involved in the modification of B27+ AS- lymphocytes by certain enteric antigens.
D000942:Antigens, Bacterial / Q000276:immunology*; D000963:Antimetabolites / Q000494:pharmacology; D003470:Culture Media; D004306:Dose-Response Relationship, Immunologic; D005374:Filtration; D006680:HLA Antigens / Q000032:analysis*; D015796:HLA-B27 Antigen; D006801:Humans; D007709:Klebsiella / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000942:Antigens, Bacterial; D000963:Antimetabolites; D003470:Culture Media; D006680:HLA Antigens; D015796:HLA-B27 Antigen