Na+ (Li+)-proline cotransport in Escherichia coli.
Na+ and Li+ were found to stimulate the transport of L-proline by cells of Escherichia coli induced for proline utilization. The gene product of the put P gene is involved in the expression of this transport activity since the put P+ strains CSH 4 and WG 148 show activity and the put P- strain RM 2 fails to show this cation coupled transport. The addition of proline was found to stimulate the uptake of Li+ and of Na+. Attempts to demonstrate proline stimulated H+ uptake were unsuccessful. It is concluded that the proline carrier (coded by the put P gene) is responsible for Na+ (or Li+)-proline cotransport.
D001693:Biological Transport, Active; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005838:Genotype; D007700:Kinetics; D008094:Lithium / Q000378:metabolism* / Q000494:pharmacology; D011392:Proline / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism* / Q000494:pharmacology
D011392:Proline; D008094:Lithium; D012964:Sodium