[Cell wall proteins of Escherichia coli: a substance resembling the thermolabile enterotoxin (LT)].
In ultrasonic extracts of all 19 investigated non-enterotoxigenic E. coli strains a substance (LTLS) could be detected reacting positively in all tests which are commonly used to detect specifically E. coli thermolabile enterotoxin (LT). Culture supernatants of these strains in general did not contain LTLS in detectable amounts. LTLS can be found in the whole cell, however, the membrane fraction contains the highest quantities. Released LTLS appears mainly aggregated with components of the cell wall, especially with lipopolysaccharides. This fact in combination with the very low quantities produced by the bacteria renders very difficult purification of LTLS.
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D001427:Bacterial Toxins / Q000302:isolation & purification*; D002772:Cholera Toxin / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000032:analysis*; D029968:Escherichia coli Proteins; D007082:Ileum / Q000187:drug effects; D011817:Rabbits; D013010:Sonication
D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli
Proteine der Zellwand von Escherichia coli: eine dem thermolabilen Enterotoxin (LT) ähnliche Substanz.