[Properties of T-cell growth factor obtained from diucifon-stimulated human lymphocytes].
The properties of T-cell growth factor (TCGF), obtained by diucifon (Dc) stimulation of human mononuclear cells (MNC) (TCGF-Dc) have been studied. Taking into account the fact that Dc alone does not, like other TCGF inductors, cause proliferation, differences between TCGF-Dc and TCGF were suggested. Partial purification of supernatant from cells, activated by Dc was performed on Sephadex G-100 column. TCGF-Dc biological activity in these fractions was determined in the system of mitogen activated human MNC and mice thymocytes, as well as in the system of concanavalin A transformed cells. 2 peaks of TCGF-Dc activity have been revealed that are indicative of TCGF-Dc molecular mass heterogeneity. In contrast to TCGF, low molecular mass TCGF-Dc (8000-12000) and TCGF-Dc from the whole supernatant were capable of absorbing on intact human MNC. TCGF-Dc may be constantly present on MNC membrane, but TCGF-Dc fixation is not sufficient for proliferation induction, the receptor activation is necessary as well. Receptors to TCGF-Dc were suggested to consist of fixing and triggering sites.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004338:Drug Combinations / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000302:isolation & purification / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008722:Methods; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D010835:Phytohemagglutinins / Q000494:pharmacology; D013450:Sulfones / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects*; D014498:Uracil / Q000031:analogs & derivatives* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D004338:Drug Combinations; D007376:Interleukin-2; D010835:Phytohemagglutinins; D013450:Sulfones; C023367:diucifon; D014498:Uracil
Issledovanie nekotorykh svoĭstv faktora rosta T-kletok, poluchennogo iz limfotsitov cheloveka, stimulirovannykh diutsifonom.