Preparation of human salivary alpha-amylase specific monoclonal antibody.
A mouse hybridoma cell line which produced an anti-human salivary alpha-amylase monoclonal antibody was obtained by fusion between mouse spleen cells immunized with human salivary alpha-amylase and mouse myeloma cells, followed by screening the hybridoma cells by enzyme-linked immunosorbent assay. The hybridoma cell line (27-4-1) secreted IgG. The monoclonal antibody produced by the hybridoma showed no inhibitory effect on the activity of human salivary alpha-amylase. The specificity and reactivity of this monoclonal antibody were examined by determining the activities of human salivary and pancreatic alpha-amylases bound to the monoclonal antibody immobilized on polystyrene balls or by enzyme immunoassay with the monoclonal antibody conjugated with beta-D-galactosidase. The results revealed that the monoclonal antibody produced by the hybridoma cell line was specific for salivary alpha-amylase and absolutely unreactive to pancreatic alpha-amylase.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D006801:Humans; D010179:Pancreas / Q000201:enzymology; D012463:Saliva / Q000201:enzymology*; D000516:alpha-Amylases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000516:alpha-Amylases