A possible pathogenetic role of cationic proteins (CP) released by stored granulocytes in the development of pulmonary infiltrates after granulocyte transfusions.
The cationic protein (CP) content of polymorphonuclear neutrophils (PMN) prepared for transfusion is depleted after storage. The supernatants from these PMN have in vitro a PMN aggregating activity which is abolished by the preabsorption with a specific rabbit anti-human PMN CP serum. Furthermore, when the supernatants stored for few hours were injected into New Zealand White rabbits, a marked sequestration of PMN took place in the lung microvascular bed. It is suggested that PMN storage per se can cause the release of intracellular mediators of possible pathogenetic importance in the development of the pulmonary infiltrates observed after PMN transfusions.
D000328:Adult; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001793:Blood Preservation; D001798:Blood Proteins / Q000009:adverse effects / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D008168:Lung / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism* / Q000637:transplantation; D011817:Rabbits; D013997:Time Factors; D065227:Transfusion Reaction
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D007106:Immune Sera; C030509:neutrophil basic proteins