PSL, an S phase-related p55 nuclear antigen, associates transiently with chromatin.
An S phase-related nuclear 55K antigen, also designated PSL, has been characterized in various mammalian cells, using a human serum from a patient with autoimmune disorders (Barque et al., EMBO j 2 (1983) 743). In this report, we show by immunoelectron microscopy that the p55 protein associates in situ with the chromatin of rat hepatocytes. This association is a transient one, as indirect immunofluorescence studies show that PSL does not bind to individualized metaphase chromosomes. Furthermore, immunoprecipitation tests indicate that the majority of PSL is in the non-chromosomal cell fraction. These results suggest that this nuclear antigen is directly involved in the DNA replication process.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis*; D002467:Cell Nucleus / Q000276:immunology*; D011232:Chemical Precipitation; D002843:Chromatin / Q000032:analysis*; D003078:Colchicine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007120:Immunochemistry; D007399:Interphase / Q000187:drug effects; D008099:Liver / Q000276:immunology / Q000648:ultrastructure; D008677:Metaphase / Q000187:drug effects; D008854:Microscopy, Electron / Q000379:methods; D009687:Nuclear Proteins; D009698:Nucleoproteins / Q000032:analysis*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
D000941:Antigens; D001324:Autoantigens; D002843:Chromatin; D009687:Nuclear Proteins; D009698:Nucleoproteins; C044854:nuclear antigen p55, human; D003078:Colchicine