Immunocytochemical investigation of carcinoembryonic antigen expression in neuroblastoma with monoclonal and polyclonal antibodies.
Immunocytochemical localization of two monoclonal anticarcinoembryonic antigen antibodies (11-285-14; 11-359-6) which recognize different CEA epitopes, and a polyclonal anti-CEA antibody (DAKO) recognizing both carcinoembryonic antigen and nonspecific cross-reacting antigen, was studied in 59 tumor tissue samples from 18 patients with neuroblastoma. No evidence of intact tumor cell staining was seen with any of the antibodies although the polyclonal antibody often stained necrotic tissue and infiltrating inflammatory cells. It is concluded that at the level of sensitivity of immunocytochemical localization, neuroblastoma cells do not express carcinoembryonic antigen.
D000293:Adolescent; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009447:Neuroblastoma / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen