Freeze-substitution of Drosophila heads for subsequent [3H]2-deoxyglucose autoradiography.
High resolution of [3H]2-deoxyglucose labelling was obtained in autoradiographs of Drosophila brains after freeze-substitution in anhydrous acetone at -76 degrees C. This method was applied to preparations which received visual, olfactory and mechanosensory stimulation. The autoradiographs were compared to those obtained after freeze-drying. Freeze-substitution, which has proved to be technically simple, rapid and inexpensive, yields a good quality of tissue preservation and hence is recommended for tissue dehydration prior to autoradiography.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D003847:Deoxyglucose / Q000378:metabolism; D004330:Drosophila / Q000378:metabolism*; D005724:Ganglia / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006652:Histological Techniques; D011309:Preservation, Biological / Q000379:methods
D003847:Deoxyglucose; D005947:Glucose