Detection of endogenous epidermal growth factor-like activity in the developing chick embryo.
Epidermal growth factor-like activity has been detected by radioreceptor assay and radioimmunoassay in the developing chick embryo. Very little activity could be detected prior to Day 8 of embryonic life (hatching is at Day 21). A peak of EGF activity was detectable by both assays over Days 10 to 12. The EGF activity then fell to virtually undetectable levels during Days 14 to 17. A later rise in RRA detectable EGF like activity was then observed over Days 18-20. The EGF activity from a Day 11 embryo chromatographed on high-performance liquid chromatography as a single peak, with very high recovery of activity, at a later elution position than mouse EGF or human EGF.
D000818:Animals; D001835:Body Weight; D002642:Chick Embryo / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004815:Epidermal Growth Factor / Q000032:analysis*; D011863:Radioimmunoassay; D011869:Radioligand Assay; D013997:Time Factors
D004815:Epidermal Growth Factor