Isolation and characterization of the nephritogenic antigen producing anti-tubular basement membrane disease.
Using monoclonal antibody affinity chromatography, we isolated a 48,000 mol wt, glucose-rich glycoprotein (3M-1) from collagenase-solubilized rabbit renal tubular basement membrane (SRTA). The purified 3M-1 protein is noncollagenous, and is capable of inducing anti-TBM (tubular basement membrane) antibodies and interstitial nephritis in susceptible hosts. Further, when SRTA, at a normally nephritogenic dose, was selectively depleted of 3M-1, it lost its ability to induce disease. As shown by immunofluorescent techniques, 3M-1 appears to be localized on rodent TBM to the exclusion of the glomerular basement membrane, but was lacking in the TBM of the LEW rat, a strain devoid of the relevant antigen of anti-TBM disease. Immunoelectron microscopy revealed that 3M-1 was associated with the most lateral aspect of the TBM, which borders, and lies in the interstitium. These results indicate that 3M-1 is the nephritogenic antigen producing experimental anti-TBM disease.
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000008:administration & dosage; D001324:Autoantigens / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification*; D001485:Basement Membrane / Q000276:immunology / Q000648:ultrastructure; D002846:Chromatography, Affinity; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000008:administration & dosage; D006168:Guinea Pigs; D026541:Heymann Nephritis Antigenic Complex; D007684:Kidney Tubules / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D009395:Nephritis, Interstitial / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D011817:Rabbits; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens; D000954:Antigens, Surface; D001324:Autoantigens; D006023:Glycoproteins; D026541:Heymann Nephritis Antigenic Complex; C035812:renal tubular antigen