Transforming growth factors (TGFs) produced by R-XC cells.
Chemical fractionation as a first step of purification of TGFs from R-XC cells and culture medium was described. Physicochemical treatment indicated that the crude preparation of RGF's contains a heat and acid stable and dithiothreitol and trypsin sensitive polypeptides related with the alpha and/or beta TGFs. The influence of partially purified TGFs on REF cell colony formation in soft agar was documented.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D003470:Culture Media; D004229:Dithiothreitol / Q000494:pharmacology; D005590:Fractional Precipitation; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D015291:Transforming Growth Factors; D014357:Trypsin / Q000494:pharmacology
D003470:Culture Media; D010455:Peptides; D015291:Transforming Growth Factors; D014357:Trypsin; D004229:Dithiothreitol