title: Expression of the Pseudomonas gene coding for carboxypeptidase G2 in Saccharomyces cerevisiae.
abstract: The Pseudomonas gene coding for carboxypeptidase G2 was introduced into Saccharomyces cerevisiae on an Escherichia coli/yeast shuttle vector pROG5. The level of enzyme activity obtained was independent of the orientation of the gene within the pBR322-derived tetracycline resistance gene of the vector, indicating that expression can occur from a Pseudomonas promoter in yeast.
mesh_terms: D002268:Carboxypeptidases / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005822:Genetic Vectors; D009876:Operon; D010641:Phenotype; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D002268:Carboxypeptidases