title: Interactions between lymphocytes and hematopoietic progenitor cells in normal and leukemic human bone marrow (phase contrast observations).
abstract: Single cell observations of normal and of leukemic human bone marrow cells demonstrated cell-cell interactions of lymphocytes with hematopoietic progenitor cells. In all cases lymphocytes and target cells were from the same individual. Lymphocyte-target cell interactions occurred more frequently with normal committed progenitor cells and leukemic blast cells from acute myeloid leukemia than with precursor cells of the proliferative cell pool, including myeloblasts, promonocytes, erythroblasts and megakaryocytes. Both induction of mitosis and degeneration of the progenitor cells occurred after cell-cell interaction with almost the same frequency. Acute myeloid leukemic blast cells degenerated after contact with lymphocytes with the same frequency as normal progenitor cells (i. e. in 16% of cell contacts), but especially during mitosis. In contrast, normal and regenerating bone marrow progenitor cells from myeloproliferative diseases demonstrated no degeneration after cell-cell interaction with lymphocytes during mitosis. Otherwise the induction of mitoses by lymphocyte-target cell interactions was more frequently observed in normal progenitor cells than in leukemic blasts.
mesh_terms: D001854:Bone Marrow Cells; D002450:Cell Communication; D002465:Cell Movement; D002470:Cell Survival; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology; D015470:Leukemia, Myeloid, Acute / Q000503:physiopathology; D008214:Lymphocytes / Q000502:physiology*; D008858:Microscopy, Phase-Contrast; D008938:Mitosis; D014411:Neoplastic Stem Cells / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*