Effects of inositol-1,4,5-trisphosphate injections into salamander rods.
Solitary rods were isolated by trituration of salamander (Ambystoma tigrinum) retinas. One barrel of an intracellular, double-barreled micropipette was used to record membrane voltage; the other barrel was used to pressure-inject inositol-1,4,5-trisphosphate. The injection of inositol-1,4,5 -trisphosphate induced a reversible hyperpolarization of the rod membrane. Injections of inositol-1,4,5-trisphosphate decreased the size of receptor potentials induced by dim lights. Conversely, light decreased the responses of the rod to injections of inositol-1,4,5-trisphosphate. These results suggest that inositol-1,4,5-trisphosphate might be involved in the modulation of rod membrane voltage during phototransduction.
D000818:Animals; D004594:Electrophysiology; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000494:pharmacology*; D007814:Larva; D008027:Light; D008564:Membrane Potentials; D010786:Photoreceptor Cells / Q000187:drug effects* / Q000502:physiology; D013403:Sugar Phosphates / Q000494:pharmacology*; D014562:Caudata
D007295:Inositol Phosphates; D013403:Sugar Phosphates; D015544:Inositol 1,4,5-Trisphosphate