Inhibition of idiotype-anti-idiotype reaction in particle counting immunoassay as a tentative assay of IgE.
Rabbit antibodies were raised against a purified mouse monoclonal IgG1 antibody having specificity for the D epsilon 2 determinant of human IgE. After appropriate absorption this antiserum was anti-idiotype specific and was used to set up a particle counting immunoassay for human IgE. Latex particles coated with F(ab')2 fragments of the anti-idiotypic antibodies were agglutinated by the monoclonal anti-IgE antibody (20 ng/ml). This reaction was inhibited up to 100% by human IgE and the assay took 30 min to perform with a sensitivity of 40 IU/ml. The coefficient of correlation with a routine IgE assay was r = 0.96, and the mean analytical recovery tested on 10 sera was 95.8%.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007158:Immunologic Techniques
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007130:Immunoglobulin Idiotypes; D007073:Immunoglobulin E