Spontaneous production of interleukin-1-like cytokine from a mouse osteoblastic cell line (MC3T3-E1).
We have investigated the role of interleukin-1 in the regulatory mechanisms of a bone remodeling system. Osteoblastic cell line (MC3T3-E1) established from newborn mouse calvaria spontaneously produced interleukin-1-like cytokine. Although the interleukin-1-like activity was not observed in culture supernatants of the cells during their exponential phase of growth, a most remarkable interleukin-1-like activity was detected in the supernatants of cells cultured in serum-free alpha-MEM on day 5 after the cells had formed a confluent monolayer. Gel filtration analysis indicated that the interleukin-1-like cytokine exhibits some heterogeneity in size.
D000818:Animals; D001769:Blood; D002460:Cell Line; D002850:Chromatography, Gel; D003470:Culture Media; D007375:Interleukin-1 / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010006:Osteoblasts / Q000378:metabolism*
D003470:Culture Media; D007375:Interleukin-1