Platelet tropomyosin binding protein is identical with serum albumin.
We have shown that the platelet tropomyosin binding protein described in the accompanying paper (Gerhard, M. D., DiGirolamo, P. M., and Hitchcock-DeGregori, S. E. (1985) J. Biol. Chem. 260, 3221-3227) is identical with human serum albumin. The immunological determinants are completely shared; the tryptic peptide maps are the same; the proteins comigrate on two-dimensional gels; and the amino acid sequences of the first 33 amino acids are the same. Although human serum albumin in plasma or commercially prepared protein will not bind tropomyosin-Affi-Gel 15, it will bind following purification from plasma by chromatography on hydroxylapatite.
D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D002352:Carrier Proteins / Q000097:blood*; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits; D012709:Serum Albumin / Q000032:analysis*; D014335:Tropomyosin / Q000097:blood*; D014357:Trypsin / Q000378:metabolism
D002352:Carrier Proteins; D010446:Peptide Fragments; D012709:Serum Albumin; D014335:Tropomyosin; D014357:Trypsin