Improvement of freeze-fracture autoradiography for localization of soluble substances in tissue samples.
Freeze-fracture autoradiography is accepted as an adequate technique for localization studies of soluble substances at the electron microscopical level. The method, however, involves many critical preparation steps, among them a protective carbon coating of the developed nuclear emulsion adhering to the replica. We demonstrate here that this additional carbon coating may be omitted. This simplification leads to a significant improvement of the sample yield as compared with the previously described procedures.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D005614:Freeze Fracturing; D011893:Rana esculenta; D013116:Spinal Cord / Q000032:analysis*