title: Biotechnological production and functional characterization of leukocytic cytokine(s) with cytotoxic effect on resting but not activated thymocytes.
abstract: In the present report we describe the analysis of suppressive and cytotoxic factor(s) derived from Con A-induced porcine leukocyte culture supernatant solution(s) (PCAS). The supernatant solution(s) had been produced on a biotechnological scale using porcine peripheral blood leukocytes isolated from 200 to 1000 1 of porcine blood. The supernatant solution(s) were tested for their effect during murine T cell activation. We found that crude supernatant solution(s) contain suppressive and cytotoxic factor(s) (SF) which have a similar selectivity for non-activated thymocytes as suppressive factor(s) previously detected in the supernatant solution(s) of allo-antigen-stimulated murine spleen cell cultures. SF was obtained from crude serum-free culture supernatants by fractionated ammonium-sulphate precipitation between 35 and 45% saturation. SF has the following characteristics: its release into the culture supernatant is dependent on mitogenic stimulation, it acts during early stages of T lymphocytic activation, the activity towards naive thymocytes is most likely caused by an irreversible cytolytic mechanism, and phenotypically and functionally immature thymocytes are preferentially affected through exposure to SF.
mesh_terms: D000818:Animals; D001688:Biological Products / Q000276:immunology* / Q000302:isolation & purification; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines; D003602:Cytotoxicity, Immunologic; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007108:Immune Tolerance; D007166:Immunosuppressive Agents; D007376:Interleukin-2 / Q000494:pharmacology; D037102:Lectins; D008213:Lymphocyte Activation; D019887:Peanut Agglutinin; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology; D014357:Trypsin
chemical_list: D001688:Biological Products; D016207:Cytokines; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D037102:Lectins; D019887:Peanut Agglutinin; D003208:Concanavalin A; D014357:Trypsin