Translation of human macrophage activating factor (for glucose consumption) mRNA in Xenopus laevis oocytes.
Total messenger RNA was extracted from a human T cell hybridoma, clone H-E4-9, which strongly produced macrophage activating factor for glucose consumption (MAF-G). This messenger RNA gave rise to functional MAF-G when translated in Xenopus laevis oocytes. Isoelectric focusing of culture supernatants of the mRNA-microinjected oocytes and the H-E4-9 cells revealed that the former contained MAF-Gs with isoelectric points of pH 5.0 and 3.0 while the latter contained MAF-Gs with isoelectric points of pH 5.0 and pH 3.3. Sucrose density gradient centrifugation analysis showed that MAF-G mRNA prepared from H-E4-9 cells sedimented at about 11.5 S.
D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D014982:Xenopus laevis
D008222:Lymphokines; D016215:Macrophage-Activating Factors; D012333:RNA, Messenger; D005947:Glucose