Detection of glucagon in pancreatic A-cells by monoclonal antibodies.
The production of a mouse monoclonal antibody from a hybrid myeloma and its use for the detection of glucagon in tissue sections is reported. The hybrid clone isolated after fusion of mouse myeloma cells with hyperimmune spleen cells from a mouse previously immunized with porcine glucagon allowed us a standardized and permanent source of monoclonal antibodies in a culture cell system. The monoclonal antibody (3 GL 31) specifically reacts with pancreatic A-cells in several species including pig, rabbit, tupaia belangeri and sheep. No immunoreactivity is observed against gut cells and neurons.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001666:Binding Sites, Antibody; D002415:Cats; D002417:Cattle; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005934:Glucagon / Q000032:analysis* / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007515:Islets of Langerhans / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D051381:Rats; D013552:Swine; D014417:Tupaia
D000911:Antibodies, Monoclonal; D005934:Glucagon