DNA strand break rejoining in human lymphocytes during the S phase.
Induction and repair of DNA strand breaks in asynchronous and synchronized cultures of human lymphocytes was investigated by using the alkaline DNA-unwinding technique followed by chromatography on hydroxylapatite. Strand break rejoining in exponentially growing human PHA stimulated lymphocytes, irradiated with 20 Gy of X-rays, is temperature-dependent, being fast at 37 degrees C (half-time of a few minutes), and very slow at around 4 degrees C. In synchronized cells irradiated with the same X-ray dose, the repair capacity increases during S phase reaching its maximum when DNA is entirely duplicated.
D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007399:Interphase; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000000981:diagnostic imaging*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011859:Radiography; D013997:Time Factors
D010835:Phytohemagglutinins