Cell cycle-related expression of the receptor for a B cell differentiation factor.
Cloned, neoplastic B cells (BCL1) have been used to evaluate the expression of the receptor for the B cell differentiation factor, BCDF mu. These cells do not secrete IgM before stimulation with BCDF mu-containing T cell supernatants (SN). By inducing cell cycle synchrony in this homogeneous population, the expression of the BCDF mu receptor could be evaluated as a function of the cell cycle. Responsiveness to BCDF mu-containing SN is maximal when the cells are in S and G2 phases of the cell cycle, and a 2-hr exposure of cells to BCDF mu-containing SN during S/G2 results in optimal IgM secretion 5 days later. Cells in S/G2 are also maximally effective in absorbing BCDF mu activity from SN. These data support the hypothesis that B cells do not respond to differentiative signals until after they are committed to at least one round of cell division.
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D002999:Clone Cells / Q000166:cytology; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D015847:Interleukin-4; D007399:Interphase; D007942:Leukemia, Experimental / Q000276:immunology; D008222:Lymphokines / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013997:Time Factors
D006133:Growth Substances; D008222:Lymphokines; D011947:Receptors, Antigen, B-Cell; D015847:Interleukin-4