Assessment of neutrophil chemotaxis and random migration in children and adults. Comparison between filter and agarose techniques.
Polymorphonuclear leucocyte migration was studied in 16 children and 11 normal adults, both by a filter technique and under agarose. Assays were carried out in parallel. There were no significant differences (p greater than 0.5) between adult controls and children's mean neutrophil migration coefficients within methods, but highly significant differences were found between methods (p less than 0.001) both in adult and children's samples. The observed intermethod differences are shown to be the result of increased random migration under agarose in both age groups.
D000328:Adult; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D002648:Child; D002675:Child, Preschool; D004731:Endotoxins / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D005374:Filtration / Q000379:methods; D006801:Humans; D007223:Infant; D009504:Neutrophils / Q000276:immunology*; D012685:Sepharose; D015054:Zymosan / Q000276:immunology
D004731:Endotoxins; D015054:Zymosan; D012685:Sepharose