title: Insulin secretion from isolated pure beta cells.
abstract: Isolation of pure beta cells of the rat pancreas was achieved employing counterflow sedimentation technique (CST) followed by density gradient centrifugation technique (DGCT). The proportion of non-endocrine cells to beta cells was minimal (1 acinar cell in 296 beta cells, and 1 duct cell in 300 beta cells) with total absence of alpha and delta cells. Oxidation of D-(U-14C) glucose to 14CO2 by the isolated beta cells was linear to time. Glucagon (1, 5, or 10 nM) or arginine (1, 5 or 10 mM) produced concentration dependent insulin secretion. Thus, a highly purified preparation of isolated beta cells of rat pancreas could be obtained with excellent morphologic, metabolic and functional integrity.
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D005934:Glucagon / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D051381:Rats
chemical_list: D007328:Insulin; D005934:Glucagon; D001120:Arginine; D005947:Glucose