Mutagenicity of metal ions in bacteria.
The mutagenicity of 24 metal salts was investigated in plate incorporation and fluctuation assays with Salmonella TA strains or Escherichia coli WP2 uvrA pKm 101. Chromate(VI) and selenate(VI) ions were found to be mutagenic in plate incorporation assays employing conventional media. On the other hand, cadmium(II), beryllium(II), chromate(VI), and metavanadate(V) ions were detected in conventional fluctuation assays, indicating the importance of this technique in detection of metal mutagens. Modified culture media, with trimetaphosphate ions in place of orthophosphate as the sole phosphate source for bacterial growth, were also used in this study. The media modifications prevented precipitation of metals such as nickel and cadmium as their insoluble phosphates, and allowed detection of the mutagenicity of metavanadate ions in plate incorporation assays. However, the fluctuation technique using standard media was shown to detect a wider range of mutagenic metal ions than tests with modified media. It is notable that metaarsenite(III), arsenate(V), and nickel(II) ions were not found to be mutagenic in any of the assays although they are known to be carcinogenic and are mutagenic in other test systems. Their lack of mutagenicity in the modified media indicates that precipitation of these ions as orthophosphates is not the reason for their lack of activity in standard bacterial assays.
D001608:Beryllium / Q000633:toxicity; D002104:Cadmium / Q000633:toxicity; D002857:Chromium / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens; D012475:Salmonella / Q000187:drug effects; D012643:Selenium / Q000633:toxicity
D008670:Metals; D009153:Mutagens; D002104:Cadmium; D002857:Chromium; D012643:Selenium; D001608:Beryllium