[Erythrocyte test preparations for standardizing fluorescent antibodies and the serological diagnosis of influenza].
An erythrocytic diagnostic preparation, obtained by the sandwich method and intended for the standardization of antivirus fluorescent antibodies (FA) and the determination of specific IgM in the blood sera of patients with a view to the early serological diagnosis of influenza, has been proposed. The loading of immunosorbents with influenza virus, achieved in this preparation, approaches its theoretical limit, the virions being arranged in a single layer, and constitutes up to 0.4 hemagglutinating units per 1000 red blood cells. The investigation made on 9 commercial and 4 experimental lots of FA has demonstrated that the newly developed test preparations serve as a convenient model for the standardization of FA and the choice of their optimum working dilution in practical diagnostic procedures. In the study of 153 paired blood sera from patients with acute respiratory diseases and clinically healthy persons the proposed variant of the early diagnosis of influenza has proved to be not inferior to the hemagglutination inhibition test and the complement fixation test with respect to the main characteristics of their diagnostic value, while having the advantage over these tests due to the fact that exact laboratory diagnosis can be made by the study of single blood samples as early as on the fourth day of the disease.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000918:Antibody Specificity; D004912:Erythrocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique / Q000592:standards; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D009980:Influenza A virus / Q000276:immunology; D007251:Influenza, Human / Q000175:diagnosis*; D011817:Rabbits; D012698:Serologic Tests / Q000379:methods / Q000592:standards
D000914:Antibodies, Viral; D007075:Immunoglobulin M
Eritrotsitarnye test-preparaty dlia standartizatsii fliuorestsiruiushchikh antitel i serologicheskoĭ diagnostiki grippa.