title: Volume regulation and basolateral co-transport of sodium, potassium, and chloride ions in frog skin epithelium.
abstract: Frog skin epithelium, which is normally almost tight to chloride, acquires a basolateral leakiness to chloride during osmotic swelling. By measuring the epithelial thickness (volume) after equilibration first with half thiocyanate Ringer, and then full thiocyanate Ringer, one obtains the chloride-free volume. Partial or full recovery of the volume and cellular chloride concentration occurs only when the inside of the skin is exposed to solutions containing K as well as Na and Cl. This recovery process is totally inhibited by low concentrations of bumetanide. The data suggest a basolateral NaKCl2 co-transport.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D001826:Body Fluids / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism*; D007700:Kinetics; D011188:Potassium / Q000378:metabolism*; D011896:Rana temporaria; D012867:Skin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters; D014882:Water-Electrolyte Balance
chemical_list: D002352:Carrier Proteins; D002712:Chlorides; D028021:Sodium-Potassium-Chloride Symporters; D012964:Sodium; D011188:Potassium