Fluorescent microsphere detection of surface antigens and simultaneous cytochemistries in individual hematopoietic cells.
A method for simultaneous analysis of cell surface antigens, cell morphology, and endogenous cytochemical staining on the same hematopoietic cell has been developed. Individual cells in heterogeneous populations can be tested for cell surface antigens using monoclonal antibodies conjugated to fluorescein-containing microspheres and subsequently stained for myeloperoxidase, Sudan black B, chloroacetate esterase, or alpha naphthyl butyrate. This technic, when applied to mixed cell populations such as normal peripheral blood and leukemic bone marrow samples, provides simultaneous analysis of cell surface antigens and specific cytoplasmic cytochemistries within individual cells. Application of this technic may increase the body of knowledge about hematopoiesis by allowing individual cells to be analyzed simultaneously for multiple lineage-associated characteristics.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D019793:Fluorescein; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007938:Leukemia / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000276:immunology; D008722:Methods; D008863:Microspheres
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005452:Fluoresceins; D019793:Fluorescein