title: Energy dispersive X-ray microanalysis of zinc and calcium in organelles of insulin-producing cells of the mouse, rat, and a fish.
abstract: By means of energy dispersive X-ray microanalysis in the scanning-transmission electron microscope, spectra were obtained from quick-frozen, cryo-ultramicrotome cut, freeze-dried sections of insulin cells from a fish and a mouse. It was shown that both zinc and calcium are present in significant quantities in native islet-cell beta-granules. In the beta-granules of the rat RINm5F insuloma cells calcium, but not zinc, seemed to accumulate; the zinc contents in the secretion granules of these neoplastic beta-cells were probably below the detection limit.
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis*; D004577:Electron Probe Microanalysis; D005399:Fishes; D007340:Insulinoma / Q000032:analysis; D007515:Islets of Langerhans / Q000032:analysis*; D051379:Mice; D009940:Organoids / Q000032:analysis; D051381:Rats; D015032:Zinc / Q000032:analysis*
chemical_list: D015032:Zinc; D002118:Calcium