Practical assessment of the NBT-PABA pancreatic function test using high performance liquid chromatography determination of p-aminobenzoic acid in urine.
A practical method for determining p-aminobenzoic acid in urine by high-performance liquid chromatography has been assessed. The technique is quick, requires no extraction steps and has good precision. Using this method, healthy individuals had a p-aminobenzoic acid excretion index of 94% +/- 18 (mean +/- 1 SD). Patients with proven, severe chronic exocrine pancreatic dysfunction had index values of less than 5-25%. Unlike chemical methods available, there were no interferences in any of the urines tested, nor did 12 compounds tested interfere with the analysis.
D010129:4-Aminobenzoic Acid / Q000652:urine*; D062365:Aminobenzoates / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D006801:Humans; D006868:Hydrolysis; D010179:Pancreas / Q000503:physiopathology*; D010182:Pancreatic Diseases / Q000503:physiopathology*; D010186:Pancreatic Function Tests; D010130:p-Aminohippuric Acid; D062366:para-Aminobenzoates
D062365:Aminobenzoates; D062366:para-Aminobenzoates; C006717:bentiromide; D010129:4-Aminobenzoic Acid; D010130:p-Aminohippuric Acid