Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate-cyclohydrolase-formyltetrahy dro folate synthetase. Affinity labelling of the dehydrogenase-cyclohydrolase active site.
Methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase are inactivated in parallel by carbodiimide-activated folic acid in an NADP-dependent reaction. Modification with tritium-labelled reagent resulted in the incorporation of 1 mole 3H-folate per mole polypeptide, which demonstrates that these activities share a single folate binding site.
D000345:Affinity Labels; D000619:Aminohydrolases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D005492:Folic Acid / Q000494:pharmacology; D005574:Formate-Tetrahydrofolate Ligase / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D045064:Methenyltetrahydrofolate Cyclohydrolase; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D011624:Pteroylpolyglutamic Acids / Q000494:pharmacology; D013552:Swine
D000345:Affinity Labels; D011624:Pteroylpolyglutamic Acids; D009249:NADP; D005492:Folic Acid; D010088:Oxidoreductases; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D045064:Methenyltetrahydrofolate Cyclohydrolase; D008025:Ligases; D005574:Formate-Tetrahydrofolate Ligase