Antigenic modulation in retinoblastoma: a flow cytometric study.
Flow cytometry (FCM) was used to investigate antigenic expression and modulation during the cell cycle of Y-79 and WERI-Rb1 tissue cultured retinoblastoma cell lines using a polyclonal anti-Y-79 antibody and fluorescein conjugated lectins. Several Y-79 resting cell populations were identified by FCM analysis of antibody binding, while only a single population with uniform antigen expression was found to exist in the synthetic and mitotic phases. WERI-Rb1 cells bound antibody approximately equally in each phase of the cell cycle. Multiple cell populations with different lectin binding affinities were seen in the resting phase with FITC-concanavalin A, FITC-ricinus communis-60 and FITC-ricinus communis-120 (FITC-RCA-120). During the S-phase of the cell cycle, a higher percentage of cells bound FITC-RCA-120 and FITC wheat germ agglutinin. The relationship between antigenic expression during the cell cycle and treatment considerations in retinoblastoma is discussed.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000941:Antigens / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D019393:Arrestin; D002453:Cell Cycle; D002460:Cell Line; D005134:Eye Neoplasms / Q000032:analysis / Q000276:immunology*; D005434:Flow Cytometry; D037102:Lectins / Q000276:immunology; D012175:Retinoblastoma / Q000032:analysis / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D000954:Antigens, Surface; D019393:Arrestin; D037102:Lectins