Motor axon sprouting in frog sartorius muscles is not altered by contralateral axotomy.
Sartorius muscles of the frog Rana pipiens were used to study the incidence of motor nerve sprouting in normal unoperated muscles, in experimental muscles contralateral to axotomy of the sartorius nerve, and in sham-operated control muscles. Muscles were stained with either a combination of nitroblue tetrazolium nerve terminal stain and cholinesterase stain or with a combination of silver nerve terminal stain and cholinesterase stain. Each endplate that could be clearly seen was classified into one or more of the following categories: normal endplates without sprouts, three types of terminal sprouts, preterminal sprouts, nodal sprouts, sprouts of unknown origin and destination, and doubly innervated gutters. A quantitative study of 318 endplates from nine unoperated muscles, 779 endplates from 45 experimental muscles, and 694 endplates from 41 control muscles showed that all muscles had a high incidence of motor nerve sprouting and other forms of remodelling (20-28% of all endplates). There were, however, no significant differences between experimental, control, and unoperated muscles when results obtained with the same stains were compared. Results obtained with the two different stains were only slightly different. We conclude that sprouting is a very common but highly variable feature of normal frog neuromuscular junctions, and in the sartorius, contralateral axotomy does not alter this ongoing remodelling.
D000818:Animals; D001369:Axons / Q000502:physiology*; D002802:Cholinesterases / Q000032:analysis; D009046:Motor Neurons / Q000502:physiology*; D009121:Muscle Denervation; D009132:Muscles / Q000502:physiology; D009416:Nerve Regeneration; D009469:Neuromuscular Junction / Q000502:physiology; D011894:Rana pipiens; D013569:Synapses / Q000502:physiology
D002802:Cholinesterases