Identification and initial characterization of transforming growth factor-like mitogen(s) in human anterior pituitary.
Transforming growth factor (TGFs) are a family of peptide(s) defined by their ability to induce anchorage-independent growth of non-neoplastic indicator cells in soft agar. We found that acid-ethanol extracts of human anterior pituitary tissues were able to stimulate colony growth of normal rat kidney fibroblasts in soft agar. When subjected to gel-filtration on a column of Bio-Gel P-60 in 1 M acetic acid, the majority of TGF activity eluted in fractions corresponding to an apparent mol wt 15,000. The activity was heat- and acid-stable, but was inactivated by treatment with trypsin and dithiothreitol. Pituitary TGF-like materials did not compete with epidermal growth factor (EGF) for receptor binding and did not require EGF for colony-forming activity. Thus, human pituitary TGF was not like type alpha or type beta TGF.
D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology; D000431:Ethanol; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008934:Mitogens / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D011869:Radioligand Assay; D015291:Transforming Growth Factors
D008934:Mitogens; D010455:Peptides; D000431:Ethanol; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D004247:DNA