Enzymatic radioimmunoassay for detecting Leptospira interrogans serovar pomona in the urine of experimentally-infected pigs.
An enzymatic radioimmunoassay (ERIA) has been developed for detecting Leptospira interrogans serovar pomona in porcine urine. Four grower pigs were experimentally infected with serovar pomona. A total of 39 urine samples was collected, and ERIA was compared with dark ground microscopy (DGM) and culture for demonstrating leptospiruria. Of 20 samples positive by at least one technique, leptospires were detected by ERIA in 14, by culture in 16 and by DGM in 13. ERIA, unlike the other 2 methods, was suitable for use with urine which had been stored frozen for several months.
D000818:Animals; D007124:Immunoenzyme Techniques; D007921:Leptospira interrogans / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis / Q000652:urine / Q000662:veterinary*; D011863:Radioimmunoassay; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000652:urine