Separation of malignant plasma cells from the bone marrow of patients with myelomatosis.
Complement-mediated lysis of bone marrow cells from patients with myelomatosis using a rabbit antiserum raised against normal peripheral blood mononuclear cells was found to greatly enrich the abnormal plasma cells. Cellular morphology was good and the cells were able to synthesize and secrete paraprotein. This was found to be a quick and useful method of preparing myeloma plasma cells for further studies of their metabolic properties.
D001853:Bone Marrow / Q000473:pathology*; D002469:Cell Separation / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D006462:Hemolytic Plaque Technique; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism; D010266:Paraproteins / Q000378:metabolism; D010950:Plasma Cells / Q000378:metabolism
D007106:Immune Sera; D009363:Neoplasm Proteins; D010266:Paraproteins