title: [Isolation and characteristics of membrane fraction of rabbit T lymphocytes specifically binding the staphylococcal enterotoxin type A].
abstract: The lymphocyte membranes from rabbit thymus were shown to bind specifically the staphylococcal enterotoxin type A (SEA). The glycolipid components were demonstrated to be absent from the SEA receptor complex on the surface of T-lymphocytes. The mild conditions were elaborated for the receptor membrane fraction solubilization by triton X-100. The affinity chromatography method was used to isolate the SEA binding membrane fraction, the major component of which is a protein with a 42,000 mol mass. The isolated preparation inhibits the specific binding of [125I]-SEA on the cellular (by T-lymphocytes) and subcellular (by membranes) levels.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002846:Chromatography, Affinity; D004768:Enterotoxins / Q000378:metabolism*; D006162:Guanylate Cyclase; D066298:In Vitro Techniques; D011817:Rabbits; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D011971:Receptors, Immunologic / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D018000:Receptors, Peptide; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D004768:Enterotoxins; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; C026066:enterotoxin A, Staphylococcal; D006162:Guanylate Cyclase; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled
vernacular_title: Vydelenie i kharakteristika membrannoĭ fraktsii T-limfotsitov krolika, spetsificheski sviazyvaiushcheĭ stafilokokkobyĭ énterotoksin tipa A.