Qualitative and quantitative Con A binding to mouse lymphocytes, T cell enriched spleen cells and allogeneically stimulated lymphocytes.
The qualitative and quantitative Con A binding behaviour of mouse spleen cells and allogeneically stimulated lymphocytes was investigated by means of fluorescence-optical and cell-electrophoretical methods. Contrary to B lymphocytes and unstimulated cultured T lymphocytes, normal as well as allogeneically stimulated T cells show a decreased electrophoretic mobility after incubation with Con A. This decrease correlates with a higher affinity and specificity to Con A on the one side and with a functional signal of Con A on the other side. Incubation with Con A is a stimulating signal for normal T lymphocytes and a suppressive one for allogeneically stimulated lymphocytes. The 3H-thymidine incorporation of B lymphocytes and unstimulated T cells is not influenced by Con A in spite of Con A binding. The amount of bound molecules is not of decisive importance for the mitogenic cultivated properties of Con A.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D003208:Concanavalin A / Q000031:analogs & derivatives / Q000378:metabolism; D004261:DNA Replication; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
D005452:Fluoresceins; D005456:Fluorescent Dyes; D011952:Receptors, Concanavalin A; C042784:fluorescein isothiocyanate-concanavalin A; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate