Plasmodium falciparum: invasion and development in highly parasitized cultures.
We report that synchronized cultures of Plasmodium falciparum with up to 40% parasitized cells can be obtained with the use of low, red blood cell suspensions and only daily replacement of culture medium. These cultures contained not only a reduced proportion of uninfected red cells but also a population of cells with brief and equal time of exposure to culture conditions. Such high parasitemias are desirable for studies of ring-staged parasites (for which enrichment techniques are not available) and late-staged parasites when the manipulations for enrichment are inappropriate or unsuccessful.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000469:parasitology*; D005978:Glutathione / Q000494:pharmacology; D006790:Host-Parasite Interactions; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D008288:Malaria / Q000097:blood / Q000469:parasitology; D010963:Plasmodium falciparum / Q000166:cytology / Q000254:growth & development*; D013535:Suspensions
D003470:Culture Media; D007042:Hypoxanthines; D013535:Suspensions; D019271:Hypoxanthine; D005978:Glutathione