[Effect of somatotropin and prolactin on functional activity and proliferation of cultured islet cells of newborn rats].
A study was made of the STH and prolactin effect on insulin secretion and 3H-thymidine incorporation in DNA by keeping them in 10.5 and 1% embryonic calf serum using a model of the primary monolayer culture of isolated pancreatic islet cells. It has been shown that STH preincubation of the islet cells for 16 hours resulted in a distinct stimulation of insulin secretion and cell proliferation in culture. Prolactin had a similar stimulating effect with STH preincubation of the cells for 24 hours. The STH and prolactin effect depended on the cultivation conditions and manifested itself to a large extent with a low content in the medium of the embryonic calf serum (1%). The results are indicative of the presence of the STH and prolactin direct specific effect on insulin secretory function and cultivated islet cell proliferation.
D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D006728:Hormones / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D006728:Hormones; D007328:Insulin; D011388:Prolactin; D013006:Growth Hormone
Vliianie somatotropnogo gormona i prolaktina na funktsional'nuiu aktivnost' i proliferatsiiu ostrovkovykh kletok v kul'ture podzheludochnoĭ zhelezy novorozhdennykh krys.