Size and domain structure of collagen VI produced by cultured fibroblasts.
Disulfide-bonded forms of collagen VI were analyzed by immunoblotting of fibroblast culture medium and cell extracts. The protein consists of pepsin and collagenase-resistant domains of about equal size indicating a molecular mass of 340 kDa for collagen VI monomers.
D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D004220:Disulfides; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010446:Peptide Fragments
D004220:Disulfides; D010446:Peptide Fragments; D008623:Mercaptoethanol; D003094:Collagen; D004229:Dithiothreitol