Reference interval for serum alpha-amylase determined with p-nitrophenyl-alpha-D-maltoheptaoside as a substrate.
The reference interval for the catalytic concentration of alpha-amylase (EC 3.2.1.1) in serum was determined in 326 blood donors. The analytic method used p-nitrophenyl-alpha-D-maltoheptaoside as substrate, which allows a continuous colorimetric measurement at 37 degrees C of the catalytic concentration of alpha-amylase in serum. The limits obtained were 0.7-3.8 mukat/l (42-228 U/l), which were in agreement with the only previous report found.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001782:Blood Donors; D005260:Female; D005960:Glucosides; D006027:Glycosides; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012737:Sex Factors; D000516:alpha-Amylases / Q000097:blood*
D005960:Glucosides; D006027:Glycosides; C036167:4-nitrophenyl maltoheptaoside; D000516:alpha-Amylases