title: Contractile proteins in pericytes. I. Immunoperoxidase localization of tropomyosin.
abstract: In these studies we have compared the relative amounts and isoforms of tropomyosin in capillary and postcapillary venule pericytes, endothelial cells, and vascular smooth muscle cells in four rat microvascular beds: heart, diaphragm, pancreas, and the intestinal mucosa. The results, obtained by in situ immunoperoxidase localization, indicate that (a) tropomyosin is present in capillary and postcapillary venule pericytes in relatively high concentration; (b) the tropomyosin content of pericytes appears to be somewhat lower than in vascular smooth muscle cells but higher than in endothelia and other vessel-associated cells; and (c) pericytes, unlike endothelia and other nonmuscle cells, contain detectable levels of tropomyosin immunologically related to the smooth muscle isoform. These results and our previous findings concerning the presence of a cyclic GMP-dependent protein kinase (Joyce, N., P. DeCamilli, and J. Boyles, 1984, Microvasc. Res. 28:206-219) in pericytes demonstrate that these cells contain significant amounts of at least two proteins important for contraction regulation. Taken together, the evidence suggests that pericytes are contractile elements related to vascular smooth muscle cells, possibly involved, as are the latter, in the regulation of blood flow through the microvasculature.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001921:Brain; D002196:Capillaries / Q000648:ultrastructure; D000070559:Gizzard, Non-avian; D007124:Immunoenzyme Techniques; D008297:Male; D008833:Microcirculation / Q000166:cytology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D014335:Tropomyosin / Q000378:metabolism*; D014699:Venules / Q000648:ultrastructure
chemical_list: D014335:Tropomyosin