An enzymatic determination of D-glucaric acid by conversion to pyruvate.
A method was developed for determination of D-glucaric acid by treatment with a bacterial extract containing glucarate dehydratase and ketodeoxyglucarate aldolase. This led to the quantitative formation of pyruvate, which was then assayed by use of lactate dehydrogenase. Measurements of D-glucarate in individual samples of human urine by this technique were compared with those by the commonly used method of beta-glucuronidase inhibition, and gave values for D-glucarate content which were about 25% higher, but with otherwise good correlation.
D000328:Adult; D000818:Animals; D001681:Biological Assay; D004926:Escherichia coli / Q000201:enzymology; D005260:Female; D005937:Glucaric Acid / Q000032:analysis* / Q000652:urine; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D006801:Humans; D006836:Hydro-Lyases; D008297:Male; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000032:analysis* / Q000096:biosynthesis; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D013400:Sugar Acids / Q000032:analysis*
D011773:Pyruvates; D013400:Sugar Acids; D009243:NAD; D019289:Pyruvic Acid; D005966:Glucuronidase; D006836:Hydro-Lyases; C020572:glucarate dehydratase; D005937:Glucaric Acid