Effect of pH, temperature, and potassium sorbate on amino acid uptake in Salmonella typhimurium 7136.
The effect of sorbate on L-serine and L-histidine uptake in Salmonella typhimurium was studied at various pH levels, temperatures, and amino acid and sorbate concentrations. Low pH had an apparent synergistic effect on amino acid uptake inhibition caused by sorbate. The relationship between sorbate concentration and the amount of amino acid uptake inhibition was not linear. Compared with L-histidine, L-serine uptake was more sensitive to changes in pH, temperature, and sorbate concentration. Various degrees of amino acid uptake inhibition by sorbate may be related to differences between amino acid transport systems. The results of this study suggest that sorbate acts as a noncompetitive inhibitor of amino acid uptake in S. typhimurium.
D000596:Amino Acids / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D012486:Salmonella typhimurium / Q000378:metabolism*; D013011:Sorbic Acid / Q000494:pharmacology*; D013696:Temperature
D000596:Amino Acids; D005231:Fatty Acids, Unsaturated; D013011:Sorbic Acid