Evidence for existence of inactive arterial renin-like enzyme in the rat.
Inactive renin-like enzyme(s) in the arterial wall of the rat are converted to active renin-like enzyme in vitro by either "acid activation" (dialysis to pH 3.3 followed by dialysis to pH 7.4) or "protease-induced activation" (trypsin, alpha-chymotrypsin and glandular kallikrein). The molecular weights of the inactive renin-like enzyme(s) before trypsin activation were estimated to be about 68,000, 44,000, 36,000 and 30,000 by column chromatography. These findings may offer a new aspect for the role of the arterial renin-angiotensin system in the local control of vascular tone by interconversion of the inactive to the active renin-like enzymes in the arterial wall.
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D001794:Blood Pressure; D004789:Enzyme Activation / Q000187:drug effects; D006851:Hydrochloric Acid / Q000494:pharmacology; D008297:Male; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000494:pharmacology / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000378:metabolism*; D012084:Renin-Angiotensin System
D010447:Peptide Hydrolases; D012083:Renin; D006851:Hydrochloric Acid