A novel immunoassay with direct relevance to protection against organophosphate poisoning.
Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase against organophosphate poisoning. The toxicity of paraoxon was reduced in mice which were preinjected with the immune sera.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001667:Binding, Competitive; D007106:Immune Sera; D007158:Immunologic Techniques; D008722:Methods; D051379:Mice; D062025:Organophosphate Poisoning; D010261:Paraoxon / Q000276:immunology / Q000633:toxicity; D011817:Rabbits
D000906:Antibodies; D007106:Immune Sera; D000110:Acetylcholinesterase; D010261:Paraoxon