Influence of selection for milk yield on endogenous hormones and metabolites in Holstein heifers and cows.
Plasma concentrations of prolactin (PRL), growth hormone (GH), insulin, glucagon, glucose, urea and free fatty acids (FFA) were measured in Holstein calves, yearlings, bred heifers and primiparous cows, either sired by bulls with high predicted differences (PD) for milk (selection group) or by bulls from an unselected random bred control population (control group; n = 6). Serial blood samples were collected before and after feeding for an 8-h period from 0900 to 1700 h. All animals were fed a complete feed at 1100 h and administered insulin (.6 IU/100 kg body weight) at 1400 h. Mean plasma PRL was greater in control animals after feeding and insulin administration, while GH was greater overall in selection cattle. Insulin remained elevated longer in selection animals after exogenous administration, and plasma glucagon was increased in the control group. While plasma glucose and urea were unaffected by genetic group, plasma FFA were elevated in selection group calves and primiparous cows compared with the control group. All hormones and metabolites differed among the pre- and post-feeding and insulin administration periods and also with age. Mean PRL and GH increased after feeding, while glucagon decreased after exogenous insulin. Plasma FFA declined after feeding, while urea and glucose were similar before and after feeding. Mean PRL increased and glucagon decreased with advancing age and plasma GH and insulin showed inverse relationships at different ages. Plasma FFA changes closely followed GH changes with age, while plasma glucose more closely followed insulin changes with age. Results indicate that all hormones measured and FFA responded to genetic selection for milk, and increases in GH are uniformly associated with increased genetic potential for milk yield.
D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary*; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D004435:Eating; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005934:Glucagon / Q000097:blood; D006062:Gonadotropins / Q000097:blood; D007328:Insulin / Q000097:blood; D007774:Lactation; D008892:Milk / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000097:blood; D011863:Radioimmunoassay; D012641:Selection, Genetic; D014508:Urea / Q000097:blood
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D006062:Gonadotropins; D007328:Insulin; D014508:Urea; D011388:Prolactin; D005934:Glucagon