[Maintenance stability of the pBR322 and pBR327 vector plasmids in Escherichia coli cells during multiple passages].
Stability of pBR322 and pBR327 plasmids was studied. Plasmid-containing Escherichia coli strains were grown in liquid growth medium without selection pressure. Plasmid pBR327 was shown to be more stable in E. coli CSH54 cells than pBR322. Essential heterogenity of individual plasmid-containing clones was recognized by the maintenance stability of plasmid DNA. The indicated clones with high stability failed to be cured from pBR327 plasmid by means of acridine orange. High stability of plasmid maintenance and the failure to cure cells containing this plasmid are suggested to correlate with and to be essentially determined by the cell functions.
D000165:Acridine Orange / Q000494:pharmacology; D003001:Cloning, Molecular / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors / Q000187:drug effects; D010957:Plasmids / Q000187:drug effects
D004269:DNA, Bacterial; D000165:Acridine Orange
Stabil'nost' podderzhaniia vektornykh plazmid pBR322 i pBR327 v kletkakh Escherichia coli pri mnozhestvennykh peresevakh.