Alleles of the Ly-17 alloantigen define polymorphisms of the murine IgG Fc receptor.
Antibodies specific for allelic determinants of the cell membrane alloantigen Ly-17 were found to react with genetically determined polymorphic sites on the murine IgG Fc receptor (Fc gamma R). Monoclonal antibodies specific for Ly-17.2 and the Fc gamma R detected identical populations of cells in thymus, spleen, lymph node, and bone marrow and had nearly identical reactivity with a large number of hematopoietic neoplasms. Antibodies to the Fc gamma R and either allele of Ly-17 blocked binding of rabbit IgG dimers to the Fc gamma R on spleen and bone marrow cells. Antibodies to the Fc gamma R also blocked binding of antibodies to either allele of Ly-17, whereas anti-Ly-17 antibodies only partially blocked the binding of antibodies to the Fc gamma R. Biochemical studies showed that antibodies to Ly-17 and the Fc gamma R precipitated proteins of Mr 55,000-60,000. The identity of the proteins recognized by these antibodies was confirmed by sequential immunoprecipitation.
D000483:Alleles; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000950:Antigens, Ly / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D005470:Fluorometry; D007074:Immunoglobulin G; D007519:Isoantigens / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011110:Polymorphism, Genetic; D011961:Receptors, Fc / Q000235:genetics*
D000906:Antibodies; D000950:Antigens, Ly; D007074:Immunoglobulin G; D007519:Isoantigens; D011961:Receptors, Fc