Influence of RU 41.740, a glycoprotein extract from Klebsiella pneumoniae, on the murine system. II. RU 41.740 facilitates the response to Con A in otherwise unresponsive T-enriched cells.
RU 41.740, a glycoprotein extract from Klebsiella pneumoniae, is a polyclonal B cell activator in the thymus-independent category, and both RU 41.740 and its fraction F1 induce the production of interleukin 1 (IL 1). RU 41.740 alone appears to have no direct effect on T-enriched cells. However, we show here that when given in conjunction with concanavalin A (Con A), RU 41.740 enabled nylon wool-passed T cells, which had been accessory cell depleted and lost responsiveness to Con A, to proliferate in response to Con A. Analysis of this observation indicated that RU 41.740 probably acted via a residual accessory cell population and that contact of this cell with the T cell was necessary for Con A activation of the T cell. Because both lectin/antigen and a source of IL 1 are required to stimulate accessory cell-depleted T cells, the mechanism of action of RU 41.740 in this system may be by induction of IL 1 from residual accessory cells in the nylon wool-passed, T-enriched cell population. However, two other agents that stimulate IL 1 production, lipopolysaccharide (LPS) and F1, do not enable T-enriched cells to respond to Con A in this system.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D002452:Cell Count; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis; D007711:Klebsiella pneumoniae / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology*
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D006023:Glycoproteins; D007375:Interleukin-1; D003208:Concanavalin A; C037567:glycoprotein, Klebsiella pneumoniae; D004247:DNA