The Ly-15 alloantigenic system: a genetically determined polymorphism of the murine lymphocyte function-associated antigen-1 molecule.
Serological and biochemical studies using monoclonal antibodies have demonstrated that the Ly-15 cell membrane alloantigens are polymorphic sites on the lymphocyte function-associated antigen-1 (LFA-1) molecule. Ly-15.2 and LFA-1 show identical tissue distributions, being present on all thymocytes, lymphocytes, and neutrophils, and flow cytofluorometric analysis indicated identical cell surface expression of these molecules. Identity of Ly-15.2 and LFA-1 was confirmed by immunochemical analysis. The Ly-15.2 and LFA-1 molecules have an identical heterodimeric structure of Mr 180,000 alpha chain and Mr 94,000 beta chain, which coelectrophorese on two-dimensional NaDodSO4/PAGE. Furthermore, anti-Ly-15.2 and anti-LFA-1 antibodies coprecipitate the same molecule from thymocyte lysates, and peptide mapping studies show that the Ly-15.2 and LFA-1 alpha chains are identical, as are the beta chains.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D007163:Immunosorbent Techniques; D007519:Isoantigens / Q000235:genetics*; D016169:Lymphocyte Function-Associated Antigen-1; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D011110:Polymorphism, Genetic; D013045:Species Specificity; D014018:Tissue Distribution
D000954:Antigens, Surface; D007519:Isoantigens; D016169:Lymphocyte Function-Associated Antigen-1