Evidence for separate genetic defects in C3H/HeJ and C3HeB/FeJ mice, that affect susceptibility to gram-negative infections.
Past studies have suggested a linkage between susceptibility to Salmonella typhimurium infection and the Lpsd genotype in C3H mice. Recently, this linkage was questioned by the finding that C3HeB/FeJ mice (Lpsn,Lpsn) were highly susceptible to systemic S. typhimurium infection. The present study shows a marked difference between C3H/HeJ and C3HeB/FeJ in their susceptibility to Gram-negative urinary tract infection. The number of E. coli and S. typhimurium recovered from the kidneys 24 hr after infection was 70 to 100 times higher in C3H/HeJ than in C3HeB/FeJ or C3H/HeN mice. Subsequently, in C3HeB/FeJ mice S. typhimurium multiplied to the level of C3H/HeJ mice, resulting in a shorter mean survival time of C3H/HeJ and C3HeB/FeJ compared with C3H/HeN mice. In contrast, E. coli remained localized to the urinary tract of C3H/HeJ mice but were eliminated from C3HeB/FeJ and C3H/HeN mice. Thus, experimental E. coli urinary tract infection appears to provide a method to differentiate the genetic defects of C3H/HeJ and C3HeB/FeJ mice. The results support an influence of the Lpsd genotype on clearance of Gram-negative bacteria from the kidneys of C3H mice.
D000818:Animals; D004198:Disease Susceptibility; D004927:Escherichia coli Infections / Q000209:etiology / Q000235:genetics*; D005260:Female; D008099:Liver / Q000382:microbiology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics* / Q000276:immunology; D008817:Mice, Mutant Strains; D008819:Mice, Nude; D010538:Peritonitis / Q000235:genetics / Q000401:mortality; D012481:Salmonella Infections, Animal / Q000209:etiology / Q000235:genetics*; D012486:Salmonella typhimurium; D013154:Spleen / Q000382:microbiology; D001745:Urinary Bladder Diseases / Q000209:etiology / Q000235:genetics; D014552:Urinary Tract Infections / Q000209:etiology / Q000235:genetics / Q000401:mortality