Articular cartilage cultured with catabolin (pig interleukin 1) synthesizes a decreased number of normal proteoglycan molecules.
A homogeneous preparation of catabolin from pig leucocytes caused a reversible dose-dependent (0.01-1 nM) decrease in the synthesis of proteoglycan in slices of pig articular cartilage cultured in serum-free medium. The monomers that were synthesized and secreted in the presence of catabolin had the same average hydrodynamic size and ability to aggregate as the controls, and the core protein was substituted with the same number of glycosaminoglycan chains. The chains were the same average length and charge as normal and were sulphated to the same extent as the controls. Newly synthesized extracellular proteoglycan was not preferentially degraded. A 2-3-fold increase in glycosaminoglycan synthesis occurred in control and catabolin-treated cartilage in the presence of beta-D-xyloside (1 mM), more than 80% being secreted into the medium as free chains. Decreased incorporation of sulphate was not reversed in the presence of lysosomal-enzyme inhibitors, and there was no evidence in pulse-chase experiments of increased intracellular degradation of glycosaminoglycan chains before secretion. It is concluded that catabolin-treated cartilage synthesizes a smaller number of normal proteoglycan molecules.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D002850:Chromatography, Gel; D046508:Culture Techniques; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006027:Glycosides / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D053583:Interleukin-1beta; D007424:Intracellular Fluid / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis*; D013431:Sulfates / Q000378:metabolism; D013552:Swine
D000085:Acetates; D006025:Glycosaminoglycans; D006027:Glycosides; D007375:Interleukin-1; D053583:Interleukin-1beta; D011509:Proteoglycans; D013431:Sulfates; C010807:xylosides; D019342:Acetic Acid