T cells from lymphoepithelial thymoma produce a biochemical variant of IL-2.
The supernatant of TE+ lymphocytes isolated by density gradient from a lympho-epithelial thymoma contained a biochemical variant of T cell growth factor (IL-2). This protein showed two peaks with an apparent molecular weight of 18 and 28 kd by Sephadex G 100 chromatography. Further purification was achieved by DEAE Sephacel and CM Sepharose ion-exchange chromatography. By isoelectric focusing the isoelectric point was found to differ from that of normal. IL-2. Based on these results, the IL-2 variant appears to be a new kind of lymphokine distinguishable from normal IL-2 and similar to a TCGF (L-TCGF peak II) purified from the malignant T cell clone HUT 102.
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000302:isolation & purification; D007526:Isoelectric Point; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology*; D013945:Thymoma / Q000276:immunology*; D013953:Thymus Neoplasms / Q000276:immunology*
D007376:Interleukin-2