Reaction components influencing CAMP factor induced lysis.
The reaction components and conditions affecting CAMP factor (Streptococcus agalactiae) induced lysis of target cells have been investigated. Both the amount of sphingomyelinase used and the time of preincubation with sphingomyelinase directly affected the rate of haemolysis by CAMP factor. The CAMP factor induced lysis was temperature dependent between 15 and 30 degrees C and was proportional to the amount of CAMP factor added.
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D006460:Hemolysin Proteins; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D012756:Sheep; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D013109:Sphingomyelins / Q000032:analysis; D013292:Streptococcus agalactiae; D013696:Temperature
D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D006460:Hemolysin Proteins; D013109:Sphingomyelins; D013108:Sphingomyelin Phosphodiesterase