Trypanosoma cruzi: metabolic labeling of trypomastigote sialoglycolipids.
Trypomastigote forms (infective) of Trypanosoma cruzi incorporate (3H)-palmitic acid and D-(3H)-galactose into glycolipids. Palmitic acid-labeled acidic glycolipids were partially hydrolyzed with neuraminidase. The labeling of these compounds when the intact cell surface was labeled with galactose oxidase plus NaB3H4 indicates the membrane location of the sialoglycolipids.
D000818:Animals; D001894:Borohydrides; D002462:Cell Membrane / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005692:Galactose Oxidase / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism*; D007700:Kinetics; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D014316:Tritium; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000378:metabolism*
D001894:Borohydrides; D006017:Glycolipids; D010169:Palmitic Acids; C009255:sialoglycolipids; D014316:Tritium; D019308:Palmitic Acid; C025364:sodium borohydride; D005692:Galactose Oxidase; D005690:Galactose