Pinacolyl methylphosphonochloridate: in vitro covalent binding to DNA and mutagenicity in the Ames test.
In the present study, in vitro binding of 14C-labeled pinacolyl methylphosphonochloridate (PiCl) to calf-thymus DNA has been investigated. The data reveal that, at PiCl concentrations of 0.038-0.26 mM, the binding is rapid and saturable at the extent of modification of 1 PiCl molecule per 2000 nucleotides. A similar range of PiCl concentrations was toxic, but did not produce a significant increase in mutagenicity in Salmonella typhimurium TA100 and TA98. Our results are of relevance with regard to the in vitro assessment of Soman-like organophosphates and the mutagenic risk of these agents and related pesticides.
D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D009152:Mutagenicity Tests; D009153:Mutagens; D009943:Organophosphorus Compounds / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects; D012999:Soman / Q000031:analogs & derivatives / Q000633:toxicity*
D009153:Mutagens; D009943:Organophosphorus Compounds; C044812:pinacolyl methylphosphonochloridate; D004247:DNA; D012999:Soman