Site specificities of three transfer RNA methyltransferases from yeast.
The site specificities of two distinct tRNA(m1G)methyltransferases and one tRNA(m2G)methyltransferase from yeast have been investigated by heterologous methylation and analysis of purified Escherichia coli tRNAs. The two tRNA(m1G)methyltransferases were found to be specific for sites 9 and 37, respectively. The tRNA(m2G)methyltransferase was specific for site 10. Two of the enzymes were purified by affinity chromatography on tRNA-Sepharose.
D001483:Base Sequence; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006147:Guanine / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity; D012359:tRNA Methyltransferases / Q000302:isolation & purification / Q000378:metabolism*
D005656:Fungal Proteins; D012331:RNA, Fungal; D006147:Guanine; D012343:RNA, Transfer; D012359:tRNA Methyltransferases; C023196:tRNA (guanine(10)-N(2))-dimethyltransferase; C036950:tRNA (guanine-N1-)-methyltransferase