[Isoelectric focusing of different classes of beta-lactamases in cell-free extracts of Gram-negative bacteria].
A method of electrofocusing of beta-lactamases in cell-free bacterial extracts was developed. It provided determination of the isoelectric points of beta-lactamases without preliminary preparation of the homogeneous enzyme. Isoelectrofocusing of beta-lactamases of 14 poly-resistant strains of gram-negative bacteria of six species was performed. It was found that the isoelectric points of the beta-lactamase belonging to the same class according to Richmond were different. No species specificity of the beta-lactamases of the gram-negative bacteria was observed.
D002850:Chromatography, Gel; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D013045:Species Specificity; D001618:beta-Lactamases / Q000032:analysis* / Q000145:classification
D001618:beta-Lactamases
Izoélektrofokusirovanie beta-laktamaz razlichnykh klassov v beskletochnykh ékstraktakh gramotritsatel'nykh bakteriĭ.