title: Trypanosoma brucei: analysis of relapsing populations in sensitive and resistant breeds of cattle.
abstract: The clone DiTat 1.1 of Trypanosoma brucei brucei was injected into four bovids, and clones obtained from successive waves of parasitemia were used to study the expressed variant-specific surface glycoprotein repertoire. Twenty-four clones were obtained which could be classified into 12 different variable antigen types, in addition to the clone injected, using agglutination or immunofluorescence with monospecific antisera. The variable surface glycoproteins of the 25 clones were extracted using the detergent octyl-beta-D-glucopyranoside in the presence of the protease inhibitor, N-cbz-L-phenylalaninechloromethylketone. The molecular weights varied from 52,000 to 69,000 and the pI from 5.0 to 8.8. The virulence of 14 clones representing 13 variable antigen types was ascertained in mice. The mean survival time ranged from 20.5 to 43.0 days. Clones isolated from early peaks of parasitemia in the bovid were the most virulent while clones derived from later peaks were less virulent. It seems that organisms of diminishing virulence appear in bovids, leading to self-cure of the disease. All clones were sensitive to human serum in a blood infectivity inhibition test. Antibody against all virulent clones appeared in 20 cattle (10 Zebus, 10 Baoulés) which had been injected with T. brucei DiTat 1.1. There was no evidence for parasites of high or low virulence being preferentially expressed in resistant or sensitive hosts.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology; D002417:Cattle; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis; D007113:Immunity, Innate; D051379:Mice; D008970:Molecular Weight; D014346:Trypanosoma brucei brucei / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D014354:Trypanosomiasis, Bovine / Q000276:immunology / Q000469:parasitology*; D014643:Variant Surface Glycoproteins, Trypanosoma; D014774:Virulence
chemical_list: D000953:Antigens, Protozoan; D006023:Glycoproteins; D014643:Variant Surface Glycoproteins, Trypanosoma