[Comparison of various methods for the identification of pneumococci based on optochin sensitivity].
The sensitivity against optochin of 491 strains of Streptococcus pneumoniae respectively alpha-haemolytic Streptococci was tested in optochin-plate-method and in the diffusion-test. Discs impregnated and used in wet condition and dry discs prepared in one of the participating laboratories were equal in their efficiency. The differentiation of Streptococcus pneumoniae and alpha-haemolytic Streptococci is possible by the disc-diffusion method using the following limit of inhibition zone. Streptococcus pneumoniae diameter of inhibition zone greater than 15 mm alpha-haemolytic Streptococci diameter of inhibition zone 0-15 mm The industrial production of optochin-discs can be recommended.
D001431:Bacteriological Techniques; D006801:Humans; D011008:Pneumococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D011803:Quinine / Q000031:analogs & derivatives*; D013183:Sputum / Q000382:microbiology; D013291:Streptococcus; D013296:Streptococcus pneumoniae / Q000302:isolation & purification
C017303:ethylhydrocupreine; D011803:Quinine
Vergleich verschiedener Methoden zur Identifizierung von Pneumokokken mittels Optochinempfindlichkeit.