title: Brain micro glutamic acid-rich protein is the C-terminal endpiece of the neurofilament 68-kDa protein as determined by the primary sequence.
abstract: The amino acid sequence of bovine brain micro glutamic acid-rich protein was determined by analysis of tryptic and Trimeresurus flavoviridis protease peptides of the molecule. The protein comprised 82 amino acid residues and has an Mr of 8992. The established sequence was highly homologous (90% identity) to the sequence of C-terminal 82 residues of the neurofilament 68-kDa protein from porcine spinal cord; there are differences of 8 residues which could be species-specific amino acid substitutions. This indicates that the micro glutamic acid-rich protein may arise by a restricted proteolysis of the neurofilament 68-kDa protein, with the break occuring toward the C-terminus.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D054815:Cyclic Nucleotide-Gated Cation Channels; D003600:Cytosol / Q000032:analysis; D010450:Endopeptidases / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D015260:Neprilysin; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011506:Proteins; D014357:Trypsin / Q000378:metabolism
chemical_list: C034698:CNGB1 protein, Bos taurus; D054815:Cyclic Nucleotide-Gated Cation Channels; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011506:Proteins; D010450:Endopeptidases; D014357:Trypsin; D015260:Neprilysin