Establishment and partial characterisation of a human fibrosarcoma cell line MR-83.
We describe a mycoplasma-free human fibrosarcoma cell line, MR-83, which grows readily in liquid culture and as clones in semi-solid agar with a plating efficiency of about 0.5%. It has a stable karyotype consisting of a modal number of 49-51 chromosomes, with two translocations and a deletion. The cell line shows resistance to adriamycin in semi-solid agar assay, and responds to Epidermal Growth Factor (EGF) by increased DNA synthesis, as measured by thymidine uptake.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D002999:Clone Cells / Q000473:pathology; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005354:Fibrosarcoma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007621:Karyotyping
D004815:Epidermal Growth Factor; D004317:Doxorubicin