title: Neonatal tolerance increases efficacy of antisera production.
abstract: In this investigation we demonstrate that neonatal tolerance can be used to increase the specificity of antisera. We have shown that if mice are immunized with a human B cell line then the antisera they produce also reacts strongly with human T cells. However, if the mice are first neonatally tolerized to a human T cell line and then immunized with the B cell line the antisera becomes much more specific for the B cell line. In theory this approach, of initially tolerizing the animal to uninteresting antigens, may also make it possible to produce monoclonal antibodies to rare cellular determinants more efficiently.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D006801:Humans; D007108:Immune Tolerance; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens