[Role of the stroma of the hematopoietic organs in the development of hematologic diseases. Intramedullary regulation of granulomonocytopoiesis].
Bone marrow trepanobiopsies were cultivated in the agar drop-liquid medium system. A total of 155 subjects with different diseases of the blood system and 31 controls were examined. It was shown that prolonged cultivation of trepanobiopsies makes it possible to functionally evaluate the stromal elements of the bone marrow. The whole medullary tissue is characterized by marked heterogeneity as regards its ability to synthesize humoral stimulants of granulomonocytopoiesis. The colony-stimulating ability (CSA) of this tissue combines the CSA of the hemopoietic and stromal cells. In bone marrow deficiency of varying degree, the function of the bone marrow stroma is highly activated. The trepanobiopsy areas short of the hemopoietic tissue possess a higher ability to form a monolayer on the dish surface. During AML and ALL relapses, the ability of the bone marrow stroma to synthesize the humoral stimulants is lowered, whereas the ability to form monolayers is less demonstrable. In diseases associated with the development of secondary fibrosis (CMF, CML, IP), the bone marrow stroma was discovered to be less capable of synthesizing the humoral stimulants, which provides evidence against the involvement of fibrous tissue in the formation of the total CSA of the bone marrow. It is assumed that fibroblasts and fatty cells are related histogenetically and that their proliferation is controlled by humoral factors.
D001853:Bone Marrow / Q000473:pathology*; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D006098:Granulocytes / Q000378:metabolism / Q000473:pathology*; D006402:Hematologic Diseases / Q000209:etiology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000378:metabolism / Q000473:pathology*
D003115:Colony-Stimulating Factors
Rol' stromy krovetvornykh organov v razvitii nekotorykh gematologicheskikh zabolevaniĭ. Intramedulliarnaia reguliatsiia granulomonotsitopoeza.