Structural differences in the cell binding region of human fibronectin molecules isolated from cultured normal and tumor-derived human cells.
Fibronectins isolated from human plasma (pFN) and from the conditioned media of normal (N-cFN) and tumor (T-cFN) human cells were compared by cathepsin D digestion followed by immunostaining of released fragments with the monoclonal antibody 3E3, specific for the cell binding site. Two different staining patterns were obtained, one specific for pFN and N-cFN, the second common to fibronectins from the 3 different kinds of tumors studied. This indicates structural differences between N-cFN and T-cFN in the cell binding region of the fibronectin molecule.
D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002402:Cathepsin D / Q000378:metabolism; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D005354:Fibrosarcoma / Q000378:metabolism; D006801:Humans; D007158:Immunologic Techniques; D008545:Melanoma / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D012208:Rhabdomyosarcoma / Q000378:metabolism
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D010446:Peptide Fragments; D002402:Cathepsin D