Temperature-dependent structural rearrangement of apotryptophanase in potassium phosphate.
Apotryptophanase (L-tryptophan indole-lyase, EC 4.1.99.1) from Escherichia coli B/1t7A shows, in the presence of potassium phosphate, a temperature-dependent structural rearrangement which is not observed in the presence of sodium phosphate or imidazole plus KC1. This rearrangement can be described by a two-state equilibrium between two forms of the apoenzyme. The midpoint temperature of the rearrangement (TM) and the van't Hoff enthalpy (delta H) at different potassium phosphate concentrations and pH values, respectively, were determined by measuring the temperature-dependence of the ultraviolet absorbance of apotryptophanase. Increasing the potassium phosphate concentration at pH 7.8 causes a simultaneous increase in total absorbance and the delta H value, whereas the TM increases between pH 7.0 and 7.8 but starts to decrease at pH values above 7.8. In 0.1 M potassium phosphate at the pH optimum of the enzyme (7.8) TM and delta H were found to be 293.1 K and 167 kJ X mol-1, respectively. Moreover, the tyrosine residues of apotryptophanase dissociate in potassium phosphate and in imidazole plus KCl with pK values of 8.6 and 9.8, respectively, indicating that potassium phosphate favors the formation of tyrosinate. The rearrangement might be interpreted as the formation of specific hydrogen bonds between tyrosine and potassium phosphate which are ruptured at higher temperature. Such hydrogen bonds cannot be formed at all or only to a small extent in the presence of imidazole plus KCl or sodium phosphate. Those hydrogen bonds stabilize the structure of apotryptophanase. In contrast, holotryptophanase requires only K+ for enzymatic activity.
D001051:Apoenzymes / Q000378:metabolism; D002021:Buffers; D004926:Escherichia coli / Q000201:enzymology*; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007700:Kinetics; D008190:Lyases / Q000378:metabolism*; D008433:Mathematics; D010710:Phosphates / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology*; D017680:Potassium Compounds; D011487:Protein Conformation; D013816:Thermodynamics; D014368:Tryptophanase / Q000378:metabolism*
D001051:Apoenzymes; D002021:Buffers; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D010710:Phosphates; D017680:Potassium Compounds; C013216:potassium phosphate; D008190:Lyases; D014368:Tryptophanase; D011188:Potassium