On mutation fixation resulting from nitrosoguanidine-induced DNA damage in Escherichia coli K12 ada-.
Brief treatment with nitrosoguanidine of E. coli defective in the removal of the pre-mutagenic lesion, gives rise to cells that segregate mutant clones. No depletion in the number of such cells occurs for at least 4 generations of growth in liquid medium. It is concluded that pre-mutagenic lesions persist and result in copying errors by an otherwise normal replication machinery.
D000477:Alkylating Agents / Q000494:pharmacology*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D008780:Methyltransferases / Q000235:genetics / Q000502:physiology; D009154:Mutation; D019853:O(6)-Methylguanine-DNA Methyltransferase
D000477:Alkylating Agents; D004269:DNA, Bacterial; D008769:Methylnitronitrosoguanidine; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase