A marker of early amacrine cell development in rat retina.
A monoclonal antibody, HPC-1, labels only amacrine and displaced amacrine cells in adult rat retina. Reactivity with displaced amacrine cells was demonstrated by double-label immunofluorescence with HPC-1 and ganglion cell-specific reagents. HPC-1 antibody reacts with a polypeptide of apparent molecular weight 35,000. HPC-1 antibody labels migrating amacrine cells in late embryonic retinas. The results define cell-specific gene expression in relation to migration of one subclass of CNS neurons.
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002454:Cell Differentiation; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000276:immunology; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000166:cytology; D051381:Rats; D012160:Retina / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface