Cells which contain S-100 protein in Hodgkin's disease: a quantitative study.
Paraffin sections from a series of 50 lymph nodes affected by Hodgkin's disease were examined by means of the unlabelled primary antibody peroxidase-antiperoxidase method to detect those cells which contained S-100 protein. In addition, 15 lymph nodes showing reactive follicular hyperplasia were studied. A simple enumeration procedure (eyepiece graticule) was used to count the number of such cells in 20 standard 25 X microscope fields. In the specimens of nodular sclerosing Hodgkin's disease many cells positive for S-100 protein were present, in contrast to the other Rye subtypes, which showed a relative paucity. By comparison, the lymph nodes showing reactive follicular hyperplasia contained a similar number of cells containing S-100 to those seen in the nodular sclerosing lymph nodes affected by Hodgkin's disease.
D002452:Cell Count; D006689:Hodgkin Disease / Q000032:analysis* / Q000473:pathology; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000032:analysis / Q000473:pathology; D009418:S100 Proteins / Q000032:analysis*
D009418:S100 Proteins