[Cloning and the expression of the hemolysin gene of Leptospira pomona pomona in Escherichia coli].
The library of Leptospira pomona genes was obtained on phage vector AL 47.1. From this library a recombinant phage carrying the hemolysin gene was selected. The DNA fragment (7.7 kb) of this phage containing the hemolysin gene was subcloned on plasmid pUC19. E. coli clones with hybrid plasmid pDR7 were shown to be hemolytic, but the secretion of hemolysin by E. coli into the culture medium was not observed.
D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D007919:Leptospira / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D006460:Hemolysin Proteins
Klonirovanie i ékspressiia gena gemolizina Leptospira pomona pomona v Escherichia coli.