A simultaneous flow cytometric assay for c-myc oncoprotein and DNA in nuclei from paraffin embedded material.
A simultaneous flow cytometric assay for the c-myc oncoprotein and DNA in nuclei extracted from archival paraffin wax embedded clinical biopsies is presented. The nuclei were extracted by pepsin digestion after dewaxing 20 micron sections. The c-myc oncoprotein was probed with a mouse monoclonal antibody. This was raised against a synthetic peptide corresponding to a hydrophilic region of the protein predicted from the amino acid sequence. The technique is illustrated with biopsies from patients with testicular cancer and with benign and malignant neoplasms of the colon.
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002467:Cell Nucleus / Q000032:analysis; D003106:Colon / Q000032:analysis; D003110:Colonic Neoplasms / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D010232:Paraffin; D011309:Preservation, Biological; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D013736:Testicular Neoplasms / Q000032:analysis; D013737:Testis / Q000032:analysis
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D010232:Paraffin; D004247:DNA