Mating-type switching in yeast is induced by thymine nucleotide depletion.
Thymidylate biosynthesis was inhibited in a haploid heterothallic strain of Saccharomyces cerevisiae. When the treated cells were mixed with a haploid strain of the same mating-type, there was an increase in the recovery of diploid colonies. Genetic and biochemical analyses demonstrated that the diploid clones arose as a consequence of induced mating-type interconversion.
D003433:Crosses, Genetic; D004171:Diploidy; D005468:Fluorodeoxyuridylate / Q000494:pharmacology; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D007700:Kinetics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D013172:Spores, Fungal / Q000502:physiology; D013937:Thymidine Kinase / Q000235:genetics; D013942:Thymine Nucleotides / Q000378:metabolism*
D013942:Thymine Nucleotides; D005468:Fluorodeoxyuridylate; D013937:Thymidine Kinase