Protein A gold-silver staining method for light microscopic immunohistochemistry.
A new sensitive method has been established for light microscopic immunohistochemistry. It involves a protein A gold technique instead of immunoglobulin-gold method, followed by an improved procedure of physical development. In this procedure, two selected reagents were employed: bromohydroquinone, a more potent developing agent than hydroquinone, and specifically purified gum arabic solution. The results obtained by this method in the pancreatic islet tissues of the rat under a variety of histochemical control conditions have substantiated both the high specificity and fidelity of this method.
D000818:Animals; D006046:Gold; D007158:Immunologic Techniques; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism; D051381:Rats; D012834:Silver; D013205:Staphylococcal Protein A
D007328:Insulin; D013205:Staphylococcal Protein A; D012834:Silver; D006046:Gold