Phospholipid biosynthesis in human platelets. The acylation of lyso-platelet-activating factor.
Acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine acyltransferase of human platelets is membrane-bound, has a pH optimum of 7.5, is insensitive to 1 mM-Mg2+, is inhibited by 1 mM-Ca2+, and is stimulated slightly by 1 mM-EDTA. Maximal formation of 1-alkyl-2-acyl-sn-glycero-3-phosphocholine is observed at 150 microM-1-alkyl-sn-glycero-3-phosphocholine and 20 microM unsaturated fatty acyl-CoA. The transfer of unsaturated fatty acyl groups to 1-alkyl-sn-glycero-3-phosphocholine is 3-14 times slower than to 1-acyl-sn-glycero-3-phosphocholine. The CoA esters of linoleate and arachidonate, two unsaturated fatty acyl groups commonly found in platelet phospholipids, are the preferred fatty acyl group donors.
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000097:blood; D000214:Acyl Coenzyme A / Q000378:metabolism; D000215:Acylation; D001792:Blood Platelets / Q000378:metabolism*; D002413:Cations, Divalent / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D013379:Substrate Specificity
D000214:Acyl Coenzyme A; D002413:Cations, Divalent; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D004492:Edetic Acid; D008243:1-Acylglycerophosphocholine O-Acyltransferase