The SOS Chromotest, a colorimetric bacterial assay for genotoxins: procedures.
The SOS Chromotest is a quantitative bacterial colorimetric assay for genotoxins. Substantial validation is now available (Quillardet et al., 1985). We describe here in detail the tester strain as well as the effects of the variation of some parameters on the assay. We report a simple spot-test procedure as well as a new standard procedure which incorporate recent technical improvements aimed at simplifying the assay further.
D000469:Alkaline Phosphatase / Q000235:genetics; D002021:Buffers; D002463:Cell Membrane Permeability; D003470:Culture Media; D004260:DNA Repair / Q000187:drug effects*; D004274:DNA, Recombinant; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D007700:Kinetics; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis*; D009154:Mutation; D010100:Oxygen; D001616:beta-Galactosidase / Q000235:genetics
D002021:Buffers; D003470:Culture Media; D004274:DNA, Recombinant; D009153:Mutagens; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase; D010100:Oxygen