Immunohistochemical investigations of the influence of reserpine on the serotonin neuron system in the rat brain.
A peroxidase-antiperoxidase immunohistochemical method with serotonin antiserum was employed to investigate the influence of reserpine on serotonin neurons of rats which were sacrificed at various times after injection (10 mg/kg i.p.). The disappearance of serotonin immunoreactivity induced by reserpine was detected only in the perikarya after 15 min, and then rapidly proceeded to the terminals. Between 2 and 4 h, immunoreactivity completely disappeared throughout the brain. The immunoreactivity reappeared in the perikarya after 6 h, and progressed toward the terminals gradually. However, there was an obvious difference in the rate of recovery of immunoreactivity between areas. After 7 days, the immunoreactivity returned to control levels.
D000818:Animals; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D008297:Male; D009474:Neurons / Q000032:analysis* / Q000187:drug effects; D011903:Raphe Nuclei / Q000032:analysis* / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000008:administration & dosage / Q000494:pharmacology*; D012701:Serotonin / Q000032:analysis*; D013997:Time Factors
D007106:Immune Sera; D012701:Serotonin; D012110:Reserpine