Microinjected photoreactivating enzymes from Anacystis and Saccharomyces monomerize dimers in chromatin of human cells.
Photoreactivating enzymes (PRE) from the yeast Saccharomyces cerevisiae and the cyanobacterium Anacystis nidulans have been injected into the cytoplasm of repair-proficient human fibroblasts in culture. After administration of photoreactivation light, PRE-injected cells displayed a significantly lower level of UV-induced unscheduled DNA synthesis (UDS) than non-injected cells. This indicates that monomerization of the UV-induced pyrimidine dimers in the mammalian chromatin had occurred as a result of photoreactivation by the injected PRE at the expense of repair by the endogenous excision pathway. Purified PRE from yeast is able to reduce UDS to 20-25% of the UDS found in non-injected cells, whereas the in vitro more active PRE from A. nidulans gives a reduction to only 70%. This suggests that the eukaryotic enzyme is more efficient in the removal of pyrimidine dimers from mammalian chromatin than its equivalent purified from the prokaryote A. nidulans.
D002478:Cells, Cultured; D002843:Chromatin / Q000187:drug effects*; D000458:Cyanobacteria / Q000201:enzymology; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008190:Lyases / Q000494:pharmacology*; D008845:Microinjections; D011740:Pyrimidine Dimers / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D002843:Chromatin; D011740:Pyrimidine Dimers; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase