Streptomyces contain Escherichia coli-type A + T-rich promoters having novel structural features.
We describe here the isolation and characterization of a class of A + T-rich transcriptionally active sequences in the filamentous antibiotic-producing Gram-positive bacterial genus Streptomyces. These regions, which digress dramatically in base composition from the 73% G + C composition characteristic of the Streptomyces genome, promote gene expression in both Escherichia coli and Streptomyces lividans and contain the major elements that determine promoter strength in E. coli. The Streptomyces-E. coli-type promoters (SEP) also show novel structural features that include multiple direct repeats within the promoter region as well as a specific hexameric sequence in the vicinity of the mRNA start point.
D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012333:RNA, Messenger