Electron-microscopic examination of the binding of a large RNA polymerase III transcription factor to a tRNA gene.
A Saccharomyces cerevisiae RNA polymerase III transcription factor was previously shown to bind stably to tRNA genes. This transcription factor has been further purified on the basis of its large size and its binding to a S. cerevisiae tRNALeu3 gene has been examined by electron microscopy. Site-specific binding of the factor to the tRNALeu3 gene sharply bends the DNA.
D002499:Centrifugation, Density Gradient; D004271:DNA, Fungal / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005800:Genes, Fungal; D008854:Microscopy, Electron; D011485:Protein Binding; D012320:RNA Polymerase III / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*
D004271:DNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III