Complete amino acid sequence of steer liver microsomal NADH-cytochrome b5 reductase.
The complete covalent structure of liver microsomal NADH-cytochrome b5 reductase from steer liver microsomes was determined. Cleavage at methionyl bonds gave 10 peptides accounting for all the residues of the protein. Acid cleavage of the reductase at the Asp-Pro bonds gave three peptides accounting for all the CNBr peptides in the molecule. Subfragmentation of these peptides by chemical and enzymatic cleavage provided overlaps which established all the fragments in an unambiguous sequence of 300 residues, corresponding to Mr 34,110. Limited tryptic digestion cleaved reductase at residues 28 and 119, yielding a preparation having two noncovalently linked peptides having a conformation which binds flavin and retains the structural features essential for NADH-cytochrome b5 activity. A model for the secondary structure of cytochrome b5 reductase is proposed that is based on computer-assisted analysis of the amino acid sequence. In this model the beta-turns are predominant and there is some 25% alpha and 30% beta structure.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D003579:Cytochrome Reductases / Q000302:isolation & purification*; D042966:Cytochrome-B(5) Reductase; D010450:Endopeptidases; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D014357:Trypsin
D010446:Peptide Fragments; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase; D010450:Endopeptidases; D014357:Trypsin; D003488:Cyanogen Bromide