[Comparison of cytoplasmic informosome proteins with RNA-binding proteins and translation initiation factors from rabbit reticulocytes. ].
Using one-and two-dimensional electrophoresis, the free and polyribosomal informosome proteins and a preparation of total RNA-binding proteins from rabbit reticulocytes were compared. It was shown that the major proteins of free and polyribosomal informosomes are similar only to the minor components of RNA-binding proteins. On the other hand, the major RNA-binding proteins, two of which are elongation translation factors EF-1L and EF2, can be present in informosome preparations only as minor components. The major proteins of polyribosomal informosomes do not coincide in terms of electrophoretic mobility with initiation factors eIF-2, eIF-2A, eIF-3, eIF-4A and eIF-4B. The major proteins of free informosomes differ in their electrophoretic mobility from initiation factors eIF-2A, eIF-4A and eIF-4B as well as from the alpha- and beta-subunits of initiation factor eIF-2.
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D007526:Isoelectric Point; D046911:Macromolecular Substances; D008970:Molecular Weight; D010448:Peptide Initiation Factors / Q000032:analysis*; D011132:Polyribosomes / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D016601:RNA-Binding Proteins; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis*; D012261:Ribonucleoproteins
D002352:Carrier Proteins; D046911:Macromolecular Substances; D010448:Peptide Initiation Factors; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C040911:informosome
Sravnenie belkov tsitoplazmaticheskikh informosom s RNK- sviaéyvaiushchimi belkami i faktorami initsiatsii transliatsii iz retikulotsitov krolika.