Influence of serotonin on lymphokine secretion in vitro.
It was found that serotonin can induce the secretion of a chemotactic activity for polymorphonuclear granulocytes and the secretion of charge-changing in in lymphocyte cultures derived from guinea pigs and mice. The serotonin antagonists methysergide, pizotiphen, cyproheptadine and ketanserin inhibited the secretion of the serotonin-elicited factors, whereas the histamine-type 2 antagonist metiamide had no inhibitory effect. Moreover, the production of Concanavalin A-induced lymphokines (migration inhibition factor for macrophages, chemotactic factor for polymorphonuclear granulocytes and for thymocytes, charge-changing lymphokines) was suppressed in the presence of serotonin in the lymphocyte cultures. The fractionation of the supernatants derived from the serotonin-stimulated lymphocytes revealed that serotonin produced an activity with a molecular weight of 40 kDa in the presence of which the Con A-elicited response was completely inhibited. These findings support the regulatory role of serotonin with respect to immune reactions.
D000818:Animals; D002630:Chemotactic Factors / Q000096:biosynthesis; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008222:Lymphokines / Q000378:metabolism*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D008297:Male; D051379:Mice; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology
D002630:Chemotactic Factors; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D012702:Serotonin Antagonists; D003208:Concanavalin A; D012701:Serotonin