The differentiation of L5/A10 myoblast cell line (a subclone of L5 line) is controlled by changes of culture conditions.
We report here that it is possible to induce differentiation in a subline of L5 myoblast line (L5/A10) by manipulating the culture media. When L5/A10 myoblast are cultured in F14 supplemented with 10% fetal calf serum the cells grow with a division time of 12 h and reach confluency at a cell density of approximately 2.4 X 10(5) cells per cm2, without undergoing differentiation, characterized, morphologically, by formation of multinucleated fibers, and biochemically, by the synthesis of muscle specific proteins such as creatine phosphokinase or myokinase. However, cells, grown in F14 + 10% fetal calf serum, will undergo regular differentiation after a limited number of division when transferred to F14 medium supplemented with limiting concentrations (1-2%) of fetal calf serum. Investigations of the biochemistry of myoblast differentiation in cell culture will be facilitated by the availability of a cell line that can undergo differentiation under controlled conditions.
D000263:Adenylate Kinase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D003402:Creatine Kinase / Q000096:biosynthesis; D003470:Culture Media; D004247:DNA / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D009132:Muscles / Q000166:cytology*; D051381:Rats; D014357:Trypsin
D003470:Culture Media; D007328:Insulin; D004247:DNA; D003402:Creatine Kinase; D000263:Adenylate Kinase; D014357:Trypsin