Modified mutagen metabolism in Schistosoma hematobium-infested organisms.
The relationship between parasite infestation and chemical mutagen metabolism was investigated in this study. Schistosoma hematobium, long associated with increased incidence of bladder cancer in humans, was chosen as a model parasite. Urine samples, serum samples, and liver tissue extracts (S-9) from infested and control hamsters were used with the Ames Salmonella/microsome test to follow 3,3'-dichlorobenzidine (DCB), aflatoxin B1 (AFB1), and 2-acetylaminofluorene (AAF) mutagenicity. Liver S-9 preparations from infested and control hamsters showed little difference in activation potential for DCB and AFB1. Aroclor 1254-induced rat liver S-9, however, was remarkably efficient at reducing the mutagenicity of DCB. This process was reversible by beta-glucuronidase (BG). Studies on infested and control hamsters indicated increased BG activity in serum and urine. Urine concentrates (UC) from infested and control animals were not mutagenic by themselves, but did enhance the mutagenicity of AAF and DCB in the presence of S-9 and BG. Urine concentrates from infested animals showed greater enhancement of DCB mutagenicity than did UC from control animals. These data suggest that increased BG and unknown urinary factors in infested hamsters play a role in altering chemical mutagen activity.
D015073:2-Acetylaminofluorene / Q000378:metabolism / Q000633:toxicity; D015101:3,3'-Dichlorobenzidine / Q000378:metabolism / Q000633:toxicity; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001711:Biotransformation; D002273:Carcinogens / Q000097:blood / Q000378:metabolism* / Q000652:urine; D020111:Chlorodiphenyl (54% Chlorine); D006224:Cricetinae; D004195:Disease Models, Animal; D005966:Glucuronidase / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008109:Liver Diseases, Parasitic / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D009152:Mutagenicity Tests; D051381:Rats; D012548:Schistosoma haematobium; D012552:Schistosomiasis / Q000378:metabolism*
D000348:Aflatoxins; D001140:Aroclors; D002273:Carcinogens; D020111:Chlorodiphenyl (54% Chlorine); D015101:3,3'-Dichlorobenzidine; D015073:2-Acetylaminofluorene; D016604:Aflatoxin B1; D005966:Glucuronidase