[Identification of staphylococci of clinical importance: evaluation of available commercial kits and technical notes on the reduction of response time and cost of performance].
A study was carried out on 150 strains of staphylococci of human origin in order to evaluate the possibility of achieving identification that is accurate, swift and perhaps economical, with the following aims in mind: a) the testing of fast systems for the detection of coagulase of Staphyslide and Sero Stat compared with classical coagulase in a test tube with EDTA and citrate; b) the evaluation of the API Staph system by comparing identification obtained through subculturing the strains in agar P, as suggested by manufacturers, with those that have been streaked directly with material on triptose agar. All the experiments were carried out in both laboratories and in double blindness. The strains of Staphylococcus aureus were 88, 90 and 98% respectively, identified by the Staphyslide, Sero Stat and coagulase in a test tube. The same results were obtained by greatly reducing the amount of the kits reactive substance. The facts show that system can take place in the microbiological laboratory to improve screening processes. The API Staph system identified 96% of all the strains and 89% of negative coagulase. The methods used to identify through primary culture in triptose are mirrored with subculture from agar P. Using this medium to streak urine, one can expect to prepare the recognition of the isolated strains the day before.
D001431:Bacteriological Techniques / Q000191:economics / Q000295:instrumentation*; D003030:Coagulase / Q000032:analysis; D003363:Cost Control; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000191:economics; D012703:Serotyping / Q000379:methods; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology; D013997:Time Factors
D003030:Coagulase; D011933:Reagent Kits, Diagnostic
Identificazione degli stafilococchi di interesse clinico: valutazione di kits disponibili in commercio e note di tecnica sulla riduzione dei tempi di risposta e costi di esecuzione.