Cell sorting of lymphocyte subpopulations by biotinylated antibody and avidin-Sepharose beads.
A method has been developed to divide lymphocyte populations into subsets based on the affinity of avidin for biotinylated antibodies. Murine splenic lymphocytes were mixed with biotin-conjugated anti-immunoglobulin, Thy-1 or anti-Lyt antibodies. Cells bound by these reagents were adsorbed to avidin-sepharose beads and by this negative selection procedure effluent cell populations enriched for T cells, B cells or Lyt subsets were prepared reproducibly and rapidly.
D000818:Animals; D000961:Antilymphocyte Serum; D001360:Avidin; D001710:Biotin; D002469:Cell Separation / Q000379:methods*; D005260:Female; D005455:Fluorescent Antibody Technique; D008214:Lymphocytes / Q000145:classification*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012685:Sepharose; D013154:Spleen / Q000166:cytology
D000961:Antilymphocyte Serum; D001360:Avidin; D001710:Biotin; D012685:Sepharose