FSH stimulation of cytosolic protein synthesis in cultured pig Sertoli cells.
The effects of FSH on the cytosolic protein synthesis by a primary culture of immature porcine Sertoli cells were studied. Cells were cultured in a chemically-defined medium for 3 days and, on day 3, they were incubated for 40 h in another medium containing labelled amino-acids either in the presence or absence of 50 ng/ml porcine FSH (pFSH). In control cells, about 107 spots (pI in the range of 5 to 8) were identified by a two-dimensional polyacrylamide gel electrophoresis of the radiolabelled cytosolic proteins. pFSH treatment produced a marked increase of seven proteins whose molecular weights and isoelectric points are respectively comprised between 25 to 58 K and 5.5 to 5.8. In addition, pFSH treatment induced a slight but constant increase of two other proteins (mol. wt: 24 and 12 K and isoelectric point: 5.3).
D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D014176:Protein Biosynthesis; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D013552:Swine
D005640:Follicle Stimulating Hormone