Stable hyper-production of Escherichia coli beta-lactamase by Bacillus subtilis grown on a 0.5 M succinate-medium using a B. subtilis alpha-amylase secretion vector.
Extracellular production of Escherichia coli beta-lactamase by Bacillus subtilis, using a B. subtilis secretion vector constructed from its own alpha-amylase gene, was promoted when the cells were grown on LG-medium containing 0.5 M succinate under poor aeration conditions. The amount of the enzyme secreted was 50 to 60 times as large as that obtained by cultivation of the cells on LG-medium under good aeration conditions. The effect of protease-deficient mutant of B. subtilis on the enzyme production by B. subtilis was significant while that of protease inhibitors was negligible or inexistent.
D001412:Bacillus subtilis / Q000201:enzymology*; D003470:Culture Media; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D008970:Molecular Weight; D013386:Succinates; D000516:alpha-Amylases / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis*
D003470:Culture Media; D004274:DNA, Recombinant; D013386:Succinates; D000516:alpha-Amylases; D001618:beta-Lactamases