Specific removal of anti-red cell blood group activity from anti-HLA lymphocytotoxic sera and purification of anti-A and anti-B antibodies with lymphocytotoxic activity.
Lymphocytotoxic antibodies to the red cell blood groups A and B were purified from human sera by elution from synthetic immunoadsorbents (Synsorbs from Chembiomed). The lymphocytotoxic activity of the eluted antibodies was better preserved when elution was carried out at pH 11, whereas it was less stable when elution was performed at pH3. In addition, complete removal of anti-red cell blood group activity from an anti-HLA serum was easily obtained by adsorption on the corresponding Synsorb without loss of anti-HLA activity.
D000017:ABO Blood-Group System / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000302:isolation & purification*; D007983:Lewis Blood Group Antigens / Q000276:immunology*
D000017:ABO Blood-Group System; D006680:HLA Antigens; D007518:Isoantibodies; D007983:Lewis Blood Group Antigens