The encapsulation of pancreatic islets. Investigation of insulin secretion and content in vitro.
Polyelectrolyte complex capsules from cellulose sulphate can be formed by precipitation in a polycation bath. The application of this new method for encapsulation of pancreatic islets requires investigations whether and to what extent cellulose sulphate injures viability and functionality of the pancreatic islets. Islets cultures in the presence of 2% cellulose sulphate for up to 3 weeks are characterized by unchanged insulin content, secretion and biosynthesis when compared to appropriate controls.
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives* / Q000276:immunology / Q000633:toxicity; D046508:Culture Techniques; D005260:Female; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D051381:Rats
D007328:Insulin; D002482:Cellulose; C020254:cellulose sulfate