Nucleologenesis: composition and fate of prenucleolar bodies.
A time course study was conducted on nucleologenesis after release from a mitotic block in the presence and absence of actinomycin D to determine the composition and fate of prenucleolar bodies (PNBs). Prenucleolar bodies, whether naturally occurring or induced by actinomycin D treatment, stain with silver and contain phosphoproteins B23 and C23, two of the major proteins of the interphase nucleolus as determined by double label immunofluorescence with specific antibodies. The nucleolus is formed by fusion of PNBs, which subsequently "reorganize" and form internal fibrillar and peripheral granular regions. Actinomycin D prevents fusion of PNBs, which are then randomly dispersed throughout the nucleus but they still contain proteins B23 and C23. These results demonstrate that the nucleolus is formed by fusion of prenucleolar structures whose biochemical composition resembles the mature nucleolus, since PNBs contain at least two of the major nucleolar proteins.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002466:Cell Nucleolus / Q000187:drug effects / Q000648:ultrastructure*; D003609:Dactinomycin / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats
D003609:Dactinomycin