Non-toxic expression in Escherichia coli of a plasmid-encoded gene for phage T4 lysozyme.
The phage T4 gene coding for lysozyme has been cloned into a plasmid under control of the (trp/lac) hybrid tac promoter and expressed in Escherichia coli with no significant toxic effect to actively growing cells. E. coli D1210 (lacIq) transformed with this plasmid produced active T4 lysozyme at levels up to 2% of the cellular protein after induction with isopropyl-beta-D-thiogalactoside. A strain producing active lysozyme was shown to be under a selective disadvantage when co-cultured with a similar strain producing inactive lysozyme. Purified strains, however, are reasonably stable in culture and under normal storage conditions.
D004274:DNA, Recombinant; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D009113:Muramidase / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*
D004274:DNA, Recombinant; D009113:Muramidase