A method for demonstration of antibodies to Trypanosoma cruzi by using antigen-coated nitrocellulose paper strips.
A method for a rapid and accurate demonstration of antibodies to Trypanosoma cruzi by using nitrocellulose paper strips coated with antigens of the parasite was developed and evaluated. Thirty-eight serum samples from 38 individuals infected with T. cruzi had a positive result in the test. Sera from normal individuals and individuals with antibodies to Toxoplasma gondii or Leishmania braziliensis had a negative result. The test was performed in a short period of time at room temperature. The antigen-coated paper may be kept for prolonged periods of time at 4 degrees C or room temperature.
D000906:Antibodies / Q000032:analysis*; D000953:Antigens, Protozoan; D014355:Chagas Disease / Q000175:diagnosis* / Q000276:immunology; D003101:Collodion; D003429:Cross Reactions; D006801:Humans; D007891:Leishmania / Q000276:immunology; D012698:Serologic Tests / Q000379:methods; D014122:Toxoplasma / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology*
D000906:Antibodies; D000953:Antigens, Protozoan; D003101:Collodion