Expression of meta-vinculin associated with differentiation of chicken embryonal muscle cells.
We confirmed the existence of meta-vinculin, which cross-reacts immunologically with vinculin and has a slightly higher molecular weight than the latter. The immunological cross-reactivity between meta-vinculin and vinculin was confirmed with monoclonal antibodies against vinculin. Furthermore, we found that this protein is present only in either smooth or striated muscle, but is absent in non-muscular tissues and that the expression of this protein is associated with a differentiation of muscle cells either in vivo or in vitro. Microinjection experiments of fluorescently labelled vinculin and/or meta-vinculin into the cytoplasm of cultured myotubes suggested that meta-vinculin may be localized at sites similar to vinculin in muscle cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D030161:Avian Proteins; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000378:metabolism; D003429:Cross Reactions; D005779:Immunodiffusion; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D016596:Vinculin
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D030161:Avian Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; C035689:VCL protein, Gallus gallus; D016596:Vinculin; D003402:Creatine Kinase