The strain of mouse and assay conditions influence whether MuIFN-gamma primes or activates macrophages for tumor cell killing.
Investigators from two different laboratories have compared several variables in the short-term macrophage-mediated cytotoxicity assays used by each group to study the role of MuIFN-gamma in macrophage activation. The findings suggest that the capacity of MuIFN-gamma to activate macrophages without the need for a second triggering stimulus is related to assay conditions and, most especially, the strain of mouse used to provide the macrophages.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D012107:Research Design; D013045:Species Specificity
D007371:Interferon-gamma