[Detection of soluble antigens of blood group ABH using immunoenzyme analysis].
The use of the indirect ELISA techniques did not ensure the sharp differentiation of the antigens of the blood groups A and B on the polystyrene sorbent by means of heteroimmune sera, though such differentiation could be achieved by means of monoclonal antibodies. The test system known as "the lectin-antibody sandwich" was found to have the optimum sensitivity and specificity permitting the detection of soluble ABH antigens. This variant of ELISA permitted the detection of blood group A antigen both in native biological materials and in traces of blood and saliva, thus making it possible to carry out its quantitative determination.
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000941:Antigens / Q000032:analysis*; D001788:Blood Grouping and Crossmatching / Q000379:methods; D001801:Blood Stains; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D037102:Lectins / Q000032:analysis; D008297:Male; D051379:Mice; D011817:Rabbits; D012463:Saliva / Q000276:immunology; D012756:Sheep / Q000276:immunology; D012995:Solubility
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000941:Antigens; D037102:Lectins
Vyiavlenie rastvorimykh antigenov grupp krovi ABH s pomoshch'iu immunofermentnogo analiza.