Adenosine 5'-triphosphate stimulates the release of polypeptides from mitochondria.
There was release of polypeptides to the medium when mitochondria containing labeled proteins were incubated with a rat liver post-mitochondrial supernatant. The release of polypeptides increased with the amount of rat liver extract added. Addition of cycloheximide did not inhibit the effect. Heating the post-mitochondrial supernatant did not inhibit the release of mitochondrial proteins, indicating that it was due to a heat-stable factor. The factor responsible has been isolated and identified as ATP. The presence of EDTA inhibits the release of polypeptides caused by ATP and Mg2+ stimulates it. The possible role of ATP in the turnover of mitochondrial proteins is briefly discussed.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002850:Chromatography, Gel; D004492:Edetic Acid / Q000494:pharmacology; D006358:Hot Temperature; D008274:Magnesium / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis; D051381:Rats
D010455:Peptides; D011498:Protein Precursors; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D008274:Magnesium