[Hybrid plasmid with bacterial and fungal markers carrying the denV gene of T4 phage and restoring the UV-resistance of E. coli uvrA].
The hybrid plasmid pYBP2 with bacterial (ampR), yeast (LEU2) and bacteriophage T4 (denV) genes has been constructed. The plasmid transformed Escherichia coli CSR603 uvrA recA ampS leuA phr- to ampicillin resistance, leucine independence, UV-resistance similar to the one of uvrA+ recA strain. Cell-free extracts of transformed Escherichia coli cells contain low level of ultraviolet-endonuclease activity in contrast to nontransformed cells containing no enzyme.
D004706:Endodeoxyribonucleases; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects*; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005814:Genes, Viral; D005819:Genetic Markers; D009097:Multienzyme Complexes / Q000235:genetics*; D009699:N-Glycosyl Hydrolases; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013604:T-Phages / Q000235:genetics*; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D005819:Genetic Markers; D009097:Multienzyme Complexes; C032219:UV endonuclease; D004706:Endodeoxyribonucleases; D009699:N-Glycosyl Hydrolases
Gibridnaia plazmida s bakterial'nym i drozhzhevym markerami, nesushchaia gen denV faga T4 i vosstanavlivaiushchaia UF-rezistentnost' E. coli uvrA.