Salmonellas in Danish pigs: a comparison of three isolation methods.
Caecal samples from 350 Danish bacon pigs were investigated for salmonella using three methods of isolation. (1) Direct inoculation of 1 g of faeces into 10 ml of Muller-Kaufmann medium (MK medium) with addition of 0.3% Teepol 610 and subculture on Brilliant Green lactose sucrose phenol-red agar (BLSF agar) with 0.3% Teepol 610. (2) Pre-enrichment of 5 g of faeces into buffered peptone water with addition of 1% Teepol 610 followed by enrichment of 1 ml in 10 ml MK medium with 1% Teepol 610 and subculture on BLSF agar with 0.3% Teepol. (3) Incubation of 0.1 ml of the pre-enrichment (2) into 10 ml Rappaport-Vassiliadis medium (RV 10 medium) incubated at 43 degrees C, subculture on BLSF agar. The MK media with and without pre-enrichment yielded higher findings than the RV 10 media. In total, 28 (8%) of the pigs were found positive, representing 11 (7.4%) of a total of 142 herds investigated. Lymph glands were collected at a later date from six of the positive herds. Five of the herds were found positive. The number of salmonellas in the glands was low, probably less than ten per gram.
D000818:Animals; D001431:Bacteriological Techniques; D002432:Cecum / Q000382:microbiology; D003470:Culture Media; D003718:Denmark; D005243:Feces / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology; D012475:Salmonella / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000382:microbiology; D012486:Salmonella typhimurium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D012703:Serotyping; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology
D003470:Culture Media