Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA; D004274:DNA, Recombinant; D006098:Granulocytes; D000882:Haplorhini; D006801:Humans; D008264:Macrophages; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes; D014162:Transfection
D003115:Colony-Stimulating Factors; D004274:DNA, Recombinant; D012333:RNA, Messenger; D004247:DNA