[Synthesis of alkaline phosphatase in a stringent and a relaxed strain of Escherichia coli under amino acid and phosphate limitation].
Synthesis of alkaline phosphatase in Escherichia coli is derepressed under phosphate starvation in the stringent strain CP78 as well as in its relaxed counterpart CP79. During limitation of phosphate as well as of amino acids a decrease of enzyme activity is observed, especially in the relaxed strain, which can not produce ppGpp under this conditions. After phosphate limitation synthesis of ppGpp is not stimulated and the kinetics of RNA synthesis is similar in both strains. We suggest that ppGpp is not directly involved in the regulation of gene expression during phosphate starvation.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000596:Amino Acids / Q000378:metabolism*; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014644:Genetic Variation; D005947:Glucose / Q000378:metabolism; D006159:Guanosine Tetraphosphate / Q000235:genetics; D010710:Phosphates / Q000378:metabolism*
D000596:Amino Acids; D003470:Culture Media; D010710:Phosphates; D006159:Guanosine Tetraphosphate; D000469:Alkaline Phosphatase; D005947:Glucose; C018279:sodium phosphate
Synthese der alkalischen Phosphatase in einem stringent und relaxed kontrollierten Stamm von Escherichia coli nach Aminosäuren- und Phosphatlimitation.