[Immunofluorescence localization of corticoliberin neurons in the brain of pigeons].
With immunofluorescence techniques using one anti-rat or two different anti-ovine CRF, the localization of corticotropin-releasing factor (CRF) producing neurons was characterized in frozen sections of pigeon brain. Colchicine was administered intraventricularly at various day hours. The CRF neurons were localized in the telencephalon: lobus parolfactorius, nucleus (n.) accumbens, anterior commissure; in the diencephalon: n. dorso-medialis and lateralis thalami and in different structures of the hypothalamus: n. praeopticus periventricularis and medialis, paraventricularis, supraopticus medialis, lateralis, ectomamillaris and in the stratum cellulare externum. Concerning the hypothalamic localizations, results are discussed in the light of physiological studies on corticotropic regulations in pigeons. Additional populations of CRF neurons were also located in various brainstem areas substantia grisea centralis, locus caeruleus, n. tegmenti dorsalis, sensorius principalis nervi trigemini, vestibularis latetalis, solitarius, nervi hypoglossi, in the dorsal area of the n. pontis lateralis and in the n. paramedianus paragiganto--cellularis, raphes, nervi facialis, subcaeruleus and the area ventralis. These particular localizations may lead to the assumption that CRF might be involved in nervous regulations other than those related to the corticotropic function.
D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D010856:Columbidae; D003346:Corticotropin-Releasing Hormone / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D009474:Neurons / Q000032:analysis / Q000166:cytology*
D003346:Corticotropin-Releasing Hormone
Localisation par immunofluorescence des neurones à corticolibérine dans l'encéphale de pigeon.