Hapten-specific prophylaxis of murine contact sensitivity by DNP-amino acid derivatives.
In mice, the elicitation of DNFB contact skin reaction was suppressed by the intravenous injection of relatively small amounts of DNP-amino acid conjugates when given at the same time of challenge test. Maximal suppression achieved was around 70% when optimal doses of DNP-amino acid were administered. The suppressive activity of 4 different amino acids, i.e., DNP-L-lysine, DNP-S-cysteine, DNP-alanine, and DNP-glycine, was nearly the same, respectively. This inhibitory effect was hapten-specific, and it failed to suppress the strong skin reaction elicited in cyclophosphamide-pretreated mice. Using the lymphocyte culture system, low doses of DNP-amino acid also suppressed the antigen-specific proliferation of sensitized lymph node cells (T prlf). Preincubation of sensitized cells with DNP-amino acid eliminated the transfer activity of T-DH cells as demonstrated previously. The mixture of lymph node cells and spleen cells from DNP-L-lysine-treated mice in vivo also suppressed the elicitation of skin reaction when transferred to recipient mice sensitized 5 days previously. Transfer studies demonstrated that this suppression was mediated by Lyt 1.2 (+), Thy 1.2 positive cells in vivo.
D000409:Alanine / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003545:Cysteine / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000209:etiology / Q000517:prevention & control*; D004139:Dinitrofluorobenzene / Q000009:adverse effects; D004140:Dinitrophenols / Q000494:pharmacology*; D005260:Female; D005998:Glycine / Q000494:pharmacology; D006241:Haptens; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000166:cytology; D008239:Lysine / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology
D000596:Amino Acids; D004140:Dinitrophenols; D006241:Haptens; D004139:Dinitrofluorobenzene; D008239:Lysine; D003545:Cysteine; D000409:Alanine; D005998:Glycine