In vitro methylation of the elongation factor EF-Tu from Escherichia coli.
The in vitro methylation of the elongation factor EF-Tu from Escherichia coli was investigated. The methylation of newly synthesized EF-Tu was obtained using lambda rifd 18 DNA as template and S-adenosyl [methyl-3H]methionine as methyl donor. About 3 mol methyl residues were incorporated for every 10 mol EF-Tu synthesized. Analysis of the nature of the methyl-containing residues by protein hydrolysis followed by paper chromatography showed that both mono- and dimethyllysine were present. The methylation of EF-Tu was also studied separately from its synthesis by using cell-free systems with artificially undermethylated components.
D002474:Cell-Free System; D004926:Escherichia coli / Q000235:genetics*; D066298:In Vitro Techniques; D008745:Methylation; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D013997:Time Factors
D010444:Peptide Elongation Factor Tu