[Interaction of the photosystem 1 reaction center with antenna chlorophyll in a pigment-protein complex isolated from pea chloroplasts].
Using a specially developed phosporoscopic attachment to spectropolarimeter, light induced spectra of circular dichroism (CD) in region 600-750 nm were measured for a pigment protein complex of photosystem 1 (PC-1) isolated from pea chloroplast (chlorophyll : P700 = 40). Minor components at 672 and 678 nm are observed in light induced spectra besides the components of dimer splitting of P700 Qy transition at 691 and 698 nm. Haussian deconvolution of light induced CD spectra of P700 and low temperature CD spectrum of PC-1 indicates that minor components are due to forms of antenna chlorophylls Chl672 and Chl678, rotational strength of that is changed by 2-4% as a result of P700 oxidation. Long term incubation of PC-1 with Triton X-100 inhibits P700 and destroys longwave optically active chlorophyll forms. A strong relation between dichroic density of 693 nm band in CD spectrum of PC-1 and the value of light induced absorption change at 698 nm could be used to determine P700 concentration on the basis of CD spectrum of PC-1. Such a relation shows that Chl693 is an important component of photo-system 1 reaction center. It is suggested that P700 is not an isolated dimer but it is included in the local complex from 8-10 chlorophyll molecules (Chl672, Chl678, Chl686, Chl693).
D002734:Chlorophyll / Q000302:isolation & purification / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D002942:Circular Dichroism; D007887:Fabaceae; D008027:Light; D045342:Light-Harvesting Protein Complexes; D010777:Photochemistry; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010946:Plants, Medicinal
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D002734:Chlorophyll
Vzaimodeĭstvie reaktsionnogo tsentra fotosistemy 1 s antennym khlorofillom v pigment-belkovom komplekse, vydelennom iz khloroplastov gorokha.