Effect of glucose and insulin administration on hepatic adenylate energy charge and the cytosolic redox state in the neonates of normal and insulin-treated diabetic rats.
At birth and during the first 3 postnatal hours, the concentrations of hepatic ATP and the adenylate energy charge were significantly higher in neonates of insulin-treated diabetic rats compared to age-matched neonates of normal rats. However, a significant reduction in these parameters was observed after 3 postnatal hours in neonates of insulin-treated diabetic rats compared to normal rats. Administration of glucose to newborns of insulin-treated diabetic rats at birth did not prevent the lowering of hepatic ATP concentrations and the adenylate energy charge in 6-hour-old neonates. In contrast, the administration of glucose or insulin to the newborns of normal rats at birth caused a reduction in the concentration of hepatic ATP and the adenylate energy charge only at 1 h after birth. The postnatal increase in the hepatic cytosolic redox state (NAD+/NADH) in the neonates of insulin-treated diabetic rats was similar to that observed in the neonates of normal rats during the first 3 postnatal hours; however, a significant reduction in this parameter was observed only in the neonates of insulin-treated diabetic rats after the third postnatal hour. Administration of glucose to the neonates of insulin-treated diabetic rats did not prevent the decline in the cytosolic redox state in these neonates at the sixth postnatal hour.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology* / Q000627:therapeutic use; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains
D000227:Adenine Nucleotides; D007328:Insulin; D007773:Lactates; D011773:Pyruvates; D009243:NAD; D019344:Lactic Acid; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D005947:Glucose