Copper chelates of antirheumatic and anti-inflammatory agents: their superoxide dismutase-like activity and stability.
An in vitro assay is described which simultaneously tests for the superoxide dismutase-like activity of low-molecular-weight copper chelates and their stability in the presence of excess EDTA. The EDTA is introduced to simulate the competitive endogenous ligands a copper-chelating drug will encounter in plasma and other body fluids. Under the conditions of the assay, antirheumatic drugs of the D-penicillamine type are clearly distinguished both from nonsteroidal anti-inflammatories and from some drugs which chelate copper but lack antirheumatic activity.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy*; D002614:Chelating Agents / Q000494:pharmacology*; D003300:Copper; D004355:Drug Stability; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D008722:Methods; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D002614:Chelating Agents; D009580:Nitroblue Tetrazolium; D003300:Copper; D004492:Edetic Acid; D013482:Superoxide Dismutase