[Use of monoclonal antibodies for the production of a plague antibody erythrocyte diagnostic agent].
Monoclonal antibodies to Yersinia pestis capsular antigen were fixed onto the surface of formulated sheep red blood cells. The preparation thus obtained was compared with commercial antibody erythrocyte diagnosticum in the passive hemagglutination test aimed at the search for the capsular antigen in the suspensions of Yersinia pestis museum cultures and in the antigen neutralization test aimed at the search for antibodies in the sera of wild and laboratory animals having had plague. Monoclonal erythrocyte diagnosticum proved to be suitable for the detection of both the capsular antigen and antibodies. The comparison of the results of the passive hemagglutination test and the enzyme immunoassay demonstrated the presence of very close relationship between them.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000942:Antigens, Bacterial / Q000276:immunology; D001202:Ascitic Fluid / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010930:Plague / Q000175:diagnosis*; D015010:Yersinia pestis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial
Isplo'zovanie monoklonal'nykh antitel dlia proizvodstva chumnogo antitel'nogo éritrotsitarnogo diagnostikuma.