Commitment events in early G1 requirement for the synthesis of dolichol dependent glycoproteins.
Dolichol functions as a carrier of oligosaccharides to polypeptide chains in the biosynthesis of N-linked glycoproteins. It is here reported that a short (4 hours) transient exposure to tunicamycin, (a specific inhibitor of dolichol dependent glycosylation) causes a cell cycle delay in post-mitotic 3T3-cells. From kinetic point of view the delay following treatment by tunicamycin resembles the delay caused by short exposures to serum deprivation or treatment by cycloheximide, indicating that the expression of N-linked glycoproteins may be involved in the cell cycle regulation. Evidence is that the availability of dolichol may be a limiting factor in this process is also presented.
D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004224:Diterpenes / Q000502:physiology*; D004286:Dolichols / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis*; D007328:Insulin / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects; D051379:Mice; D009844:Oligosaccharides / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology
D004224:Diterpenes; D004286:Dolichols; D006023:Glycoproteins; D007328:Insulin; D009844:Oligosaccharides; D010982:Platelet-Derived Growth Factor; D014415:Tunicamycin; D004815:Epidermal Growth Factor; D003513:Cycloheximide