[Cytological and insulin-producing characteristics of cultures of pancreatic islet cells from newborn piglets].
The paper is concerned with the characteristics of morphological changes and insulin producing activity of neonatal pig islet cell initial cultures in health and in different glucose concentrations. The investigated cultures preserve morphological and functional differentiation within 4-5 weeks of growth. Insulin biosynthesis and secretion are raised by glucose injection at a dose of 300 mg%. An increase in the dose up to 600 mg% results in the suppression of insulocyte functional activity. The obtained cultures can be used for the treatment of diabetes mellitus.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003584:Cytological Techniques; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D013552:Swine / Q000502:physiology*; D013997:Time Factors
D007328:Insulin; D005947:Glucose
Tsitologicheskaia i insulinprodutsiruiushchaia kharakteristika kul'tur ostrovkovykh kletok podzheludochnoĭ zhelezy novorozhdennykh porosiat.