Immunoregulation in disseminated murine histoplasmosis: disturbances in the production of interleukins 1 and 2.
Production of IL 1 and IL 2 by splenocytes from C57BL/6 mice was measured at wk 1, 3, 8, and 14 after i.v. inoculation with 6 X 10(5) Histoplasma capsulatum (Hc) yeasts. As compared with age-matched controls, IL 1 production by splenocytes from Hc-infected mice was reduced severely at wk 1 and 3 of infection, greater than normal at wk 8, and within normal range at wk 14. IL 2 production was also reduced at wk 1 and 3 of infection; it was normal at wk 8 and was elevated at wk 14. Indomethacin and catalase failed to restore IL 1 production by splenocytes from infected mice, and exogenous IL 1 did not augment IL 2 production by these cells. A factor capable of suppressing the activity of IL 2 was detected in supernatants of concanavalin A-stimulated splenocytes from infected animals at wk 1 and 3 of infection, respectively. No factor capable of suppressing IL 1 activity was detected. Thus, the deficits of cell-mediated immunity in mice with systemic Hc infection may derive, in part, from impaired amplification of the immune response consequent to abnormal generation of IL 1 and IL 2.
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D004195:Disease Models, Animal; D006660:Histoplasmosis / Q000276:immunology*; D007163:Immunosorbent Techniques; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000502:physiology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000378:metabolism / Q000502:physiology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology
D007375:Interleukin-1; D007376:Interleukin-2; D002374:Catalase; D007213:Indomethacin