An amino acid sequence common to both cartilage proteoglycan and link protein.
Cartilage proteoglycan monomers associate with hyaluronic acid to form proteoglycan aggregates. Link protein, interacting with both hyaluronic acid and proteoglycan, serves to stabilize the aggregate structure. In the course of determining the primary structure of link protein, two peptides produced by digestion of rat chondrosarcoma link protein with trypsin or chymotrypsin have been selectively purified by immunoaffinity chromatography on a column of monoclonal anti-link protein antibody (8A4) immobilized to Sepharose 4B. These peptides have been sequenced using the double-coupling dimethylaminoazobenzene isothiocyanate/phenyl isothiocyanate procedure. A consensus sequence, Cys-X-Ala-Gly-Trp-Leu-X-Asp-Gly-Ser-Val-X-Tyr-Pro-Ile-X-X-Pro, obtained by comparing the affinity-isolated tryptic peptide with the affinity-isolated chymotryptic peptide and an overlapping tryptic peptide, shows homology with a sequence obtained from the NH2-terminal of a CNBr peptide from proteo glycan core protein of bovine nasal cartilage: Ser-Ser-Ala-Gly-Trp-Leu-Ala-Asp-Arg-Ser-Val-Arg-Tyr-Pro-Ile-Ser-. We suggest that the common sequence is structurally important to the function of these proteins and may be involved in the binding of both link protein and proteoglycan to hyaluronic acid.
D000595:Amino Acid Sequence; D000818:Animals; D002356:Cartilage / Q000032:analysis*; D002813:Chondrosarcoma / Q000032:analysis; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D007158:Immunologic Techniques; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D014357:Trypsin / Q000378:metabolism; D000099249:Proteoglycan Link Protein
D002918:Chymotrypsin; D016326:Extracellular Matrix Proteins; D010446:Peptide Fragments; D011506:Proteins; D011509:Proteoglycans; D014357:Trypsin; D000099249:Proteoglycan Link Protein