From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila.
A monoclonal antibody (MAb24B10), derived from mice immunized with Drosophila retina, exclusively stains photoreceptor cells in the retina and their axonal projections to the optic ganglia. The antigen (Ag24B10) is a 160-kDa glycoprotein comprising about 0.8% of the retina protein. By microsequencing, 19 of the first 21 amino acids at the NH2-terminal end of the protein have been determined. Using synthetic oligonucleotide probes corresponding to a portion of this amino acid sequence, we isolated a homologous lambda genomic clone. A partial DNA sequence of this clone, along with blot experiments on genomic DNA and RNA, indicate that this clone is part of the structural gene for Ag24B10. By in situ hybridization, the gene was localized to the tip of chromosome 3R.
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D009474:Neurons / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006023:Glycoproteins