Autofluorescence of alveolar macrophages: problems and potential solutions.
Despite recent advances in immunofluorescence technology, the study of alveolar macrophage cell surface proteins is hampered by the presence of autofluorescence. We suggest several approaches that might overcome this problem and enable specific immunofluorescent detection of cellular proteins and automated analysis of alveolar macrophages.
D003592:Cytophotometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D008264:Macrophages / Q000502:physiology*; D010777:Photochemistry