gamma-Interferon stimulates production of 1,25-dihydroxyvitamin D3 by normal human macrophages.
We show for the first time that normal human pulmonary alveolar macrophages (PAM) markedly enhance their basal rate of the production of [3H]-1,25(OH)2D3 when cultured in the presence of recombinant gamma-interferon (gamma-IFN). The rate of conversion of [3H]-25(OH)D3 to [3H]-1,25(OH)2D3 was dose-dependent in a linear fashion. A maximal production of 1,25(OH)2D3 by PAM occurred after exposure of PAM to gamma-IFN for one day. This maximum plateau-level was sustained for at least five days. The authenticity of the putative 1,25(OH)2D3 obtained from PAM was tested by demonstrating the exact comigration of [3H]-1,25(OH)2D3 with chemically synthesized 1,25(OH)2D3 in four different HPLC-systems.
D000818:Animals; D002117:Calcitriol / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology*; D008214:Lymphocytes / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D009000:Monocytes / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D011650:Pulmonary Alveoli / Q000166:cytology
D007371:Interferon-gamma; D002117:Calcitriol