[Quantitative determination of human bone marrow T cells following in vitro depletion by Campath-1 monoclonal antibody].
Bone marrow T cell depletion is an effective graft vs host disease prophylaxis in allogeneic bone marrow transplantation. Standard methods of identifying T cells are not sensitive enough to determine small numbers of residual T cells following T cell depletion. A rapid and sensitive method, using limiting dilution culture technology and radionucleotide labelling and allowing the detection of 1/10(4)-1/10(5) residual T cells, has been developed. Its usefulness in the evaluation of the number of T cells after monoclonal antibody (Campath-1) + autologous complement marrow treatment is demonstrated.
D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D016026:Bone Marrow Transplantation; D006086:Graft vs Host Disease / Q000517:prevention & control; D006801:Humans; D066298:In Vitro Techniques; D007958:Leukocyte Count / Q000379:methods; D008212:Lymphocyte Depletion; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal
Détermination quantitative des cellules T humaines médullaires après déplétion in vitro par l'anticorps monoclonal Campath-1.