[Methodological aspects of the determination of nitrogen metabolism parameters from 15N-tracer experiments on swine based on nitrogen metabolism models. 2. Calculation of N metabolism parameters from the time of urinary excretion of 15N].
After a 45-hour infusion of 15N-lysine and 15N-glycine resp. with pigs of 25 kg live weight, the infusion of the tracer was disrupted and a 3-day reduction phase was made part of the experiment. The N-metabolism parameters such as protein synthesis and decomposition quotas were calculated on the basis of the temporal development of 15N-amounts excreted in urine. The labelling and reduction phases were evaluated simultaneously. By means of including the total N-amount of the animal body the balance calculation becomes more stable. The possible influence of the tracer amino acid in connection with the 3-pool-model is discussed on the basis of the ascertained parameters.
D000818:Animals; D005998:Glycine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism* / Q000652:urine; D009587:Nitrogen Isotopes; D011506:Proteins / Q000378:metabolism; D013552:Swine / Q000378:metabolism*
D009587:Nitrogen Isotopes; D011506:Proteins; D008239:Lysine; D009584:Nitrogen; D005998:Glycine
Methodische Aspekte zur Bestimmung von N-Umsatzparametern aus 15N-Tracerversuchen an Schweinen auf der Grundlage von Modellen des N-Stoffwechsels. 2. Mitteilung Berechnung von N-Stoffwechselparametern aus dem zeitlichen Verlauf der im Harn ausgeschiedenen 15N-Mengen.