Induction of IgG secretion in a human B cell clone with recombinant IL 2.
A human B cell clone (SGB3) responsive to IL 2 was established. Both recombinant IL 2 and B cell differentiation factor (BCDF) induced IgG secretion in SGB3 cells in a dose-dependent manner, but IL 2 did not affect the proliferation of SGB3 cells. FACS analysis showed that SGB3 cells expressed Tac antigen and anti-Tac antibody inhibited IL 2-induced IgG secretion without any inhibitory effect on BCDF-induced IgG secretion. These results showed that IL 2 could directly act on B cells and provide a differentiation signal through IL 2 receptors distinct from BCDF receptors. Physiologic relevance of IL 2 in the antibody response was discussed.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001665:Binding Sites; D002460:Cell Line; D002999:Clone Cells / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D011971:Receptors, Immunologic / Q000032:analysis / Q000502:physiology; D015375:Receptors, Interleukin-2
D007074:Immunoglobulin G; D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2