Stimulation of calcium uptake in rat calvaria by prostacyclin.
Treatment of newborn rat calvaria discs with a variety of unsaturated fatty acids led to a 50% enhancement of calcium uptake. Arachidonic acid was effective at lower concentrations than cis-vaccenic or oleic acid, while trans-vaccenic acid and saturated fatty acids did not enhance calcium uptake. Cyclooxygenase inhibitors indomethacin and acetylsalicylic acid abolished the enhancement of calcium uptake seen in response to cis-vaccenic acid and inhibited calcium uptake by otherwise untreated bones. Prostacyclin was found to produce up to 2 fold stimulation of calcium uptake with an EC50 of approximately 0.1 microM. No statistically significant stimulation of calcium uptake was seen in response to PGE2 or PGE1 alpha up to 25 microM, while slight stimulation was produced by 6-keto PGE1 alpha but only at concentrations of 10 microM. Prostacyclin production by calvaria was demonstrated and was stimulated over 50% by cis-vaccenic acid. These results suggest that not only is enhanced prostacyclin production responsible for elevation of calcium uptake in response to unsaturated fatty acids, but also that prostacyclin may be an important regulator of bone calcium homeostasis.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors; D011464:Epoprostenol / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D051381:Rats
D016861:Cyclooxygenase Inhibitors; D005227:Fatty Acids; D011464:Epoprostenol; D001241:Aspirin; D002118:Calcium; D007213:Indomethacin