[Influence of antibiotics on the production of interleukin-1 by macrophages].
Influence of cefoxitin and cefotaxime on interleukin-1 production (IL-1) by mice peritoneal resident macrophages activated by opsonized zymosan was investigated. Evaluation of the in vivo effect was done by examining macrophages of animals following intravenous administration of 30 mg/kg cefoxitin or cefotaxime. In vitro studies consisted in testing IL-1 production by macrophages in cell cultures in the presence of either antibiotic. In vivo IL-1 production was unchanged following cefoxitin treatment of cell donors, whereas a more than threefold increase was observed after administration of cefotaxime. In vitro, IL-1 production was enhanced by both antibiotics, but the pattern and extent of this effect diverged widely. Cefoxitin in a concentration of 300 micrograms/ml induced a threefold increase in IL-1 production. IL-1 production was increased tenfold and eightfold respectively by 150 and 300 micrograms/ml cefotaxime. When the concentration of either antibiotic was very high (400 micrograms/ml), the effect was significantly less important; a certain degree of inhibition was even observed with cefotaxime. Effects of cefoxitin and cefotaxime on IL-1 production and--as shown in previous studies--on prostaglandin E2 (PGE2) production and sensitivity of immunocompetent cells to PGE2 may be important factors in the mechanism of immunological side effects which result in a modulation of the course of experimental infection.
D000818:Animals; D001202:Ascitic Fluid; D002439:Cefotaxime / Q000494:pharmacology*; D002440:Cefoxitin / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009895:Opsonin Proteins / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D007375:Interleukin-1; D009895:Opsonin Proteins; D010835:Phytohemagglutinins; D002440:Cefoxitin; D015054:Zymosan; D002439:Cefotaxime
Influence des antibiotiques sur la production de l'interleukine-1 par des macrophages.