[Enzymatic synthesis of 3,4-dihydroxyphenyl-L-alanine by free and immobilized Citrobacter freundii cells].
The Citrobacter freundii 62 cells immobilized in PAAG and possessing the tyrosine-phenol-lyase (TPL) activity catalyse the synthesis of 3,4-dihydroxyphenyl-L-alanine (DOPA) from pyrocatechol and ammonium pyruvate. The synthesis of DOPA was studied using both free and immobilized bacterial cells. When the concentration of pyrocatechol is over 0.1 M the TPL activity of the cells is inhibited. The concentration of pyrocatechol can be increased up to 0.3 M by using an equimolar mixture of pyrocatechol and boric acid. The addition of ascorbic acid as an antioxidant results in a lower TPL activity of both free and immobilized bacterial cells.
D000180:Acrylic Resins / Q000494:pharmacology; D002396:Catechols / Q000378:metabolism; D002855:Chromatography, Thin Layer; D002954:Citrobacter / Q000187:drug effects / Q000201:enzymology*; D004295:Dihydroxyphenylalanine / Q000032:analysis / Q000096:biosynthesis*; D005782:Gels; D007700:Kinetics; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D014447:Tyrosine Phenol-Lyase / Q000378:metabolism
D000180:Acrylic Resins; D002396:Catechols; D005782:Gels; D011773:Pyruvates; C016680:polyacrylamide gels; D004295:Dihydroxyphenylalanine; D019289:Pyruvic Acid; D014447:Tyrosine Phenol-Lyase
Fermentativnyĭ sintez 3,4-dioksifenil-L-alanina svobodnymi i immobilizovannymi kletkami Citrobacter freundii.