Resistance plasmids of Pasteurella multocida isolated from turkeys.
From 1940 through 1978, fifty-eight strains of Pasteurella multocida (serotype 3) were isolated from turkeys throughout the United States and were examined for R-plasmids. Forty-one of the isolates contained plasmid DNA, of which 7 isolates were found to encode resistance to tetracycline, streptomycin, and sulfonamides, or to streptomycin and sulfonamides. The R-plasmids were 2 to 10 megadaltons, nonconjugal, and contained a moles percent guanine plus cytosine ratio in the range of 57 to 61. The R-plasmids did not belong to any of the 19 incompatibility groups evaluated, including Inc Q. Digestion with restriction endonuclease indicated that 2 of the plasmids from P multocida isolated in 1960 and 1962 were identical, whereas 4 of the 5 plasmids obtained from P multocida isolated after 1966 were identical, with the 5th plasmid closely related to the other 4. The results indicated that R-plasmids were not widely dispersed among P multocida (serotype 3) isolated from turkeys in the United States. The nontransmissible nature of these plasmids was probably the major reason for their lack of dissemination.
D000818:Animals; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004270:DNA, Circular / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000032:analysis / Q000235:genetics; D006147:Guanine / Q000032:analysis; D008970:Molecular Weight; D010325:Pasteurella / Q000032:analysis / Q000235:genetics*; D011815:R Factors; D014169:Transformation, Bacterial; D014422:Turkeys / Q000382:microbiology*
D004269:DNA, Bacterial; D004270:DNA, Circular; D006147:Guanine; D003596:Cytosine