Screening expression libraries with nonradioactive immunological probes.
An immunological screening method employing protein A-peroxidase which does not require radiolabelled antibodies for detection of Escherichia coli colonies synthesizing foreign proteins in a cDNA expression library is described. The technique is sensitive, simpler and more rapid than the procedures that rely on radiolabelled antibodies and autoradiography.
D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D007158:Immunologic Techniques; D011994:Recombinant Proteins / Q000032:analysis* / Q000276:immunology; D013205:Staphylococcal Protein A; D015028:Zein / Q000235:genetics
D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D015028:Zein