[LHRH stimulates the secretion of an 87 K protein by cultured gonadotrophic cells].
The biosynthesis of proteins secreted by cultured pituitary cells was studied using (35S)-methionine. The intracellular and released proteins were analyzed by polyacrylamide gel electrophoresis followed by autoradiography. The LHRH-stimulated release is accompanied by the synthesis and release of a 87,000 dalton molecule (87 K protein). This peculiar secretion is particularly evidenced when using enriched gonadotrophs obtained by centrifugal elutriation. The 87 K protein could be involved in the release process of gonadotropins.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D007987:Gonadotropin-Releasing Hormone; D008715:Methionine
Le LHRH stimule la sécrétion d'une protéine 87 K par les cellules gonadotropes en culture.