Blastogenic response of bovine lymphocytes to Brucella abortus lipopolysaccharide.
Brucella abortus lipopolysaccharide was tested in a blastogenesis assay with unfractionated and nylon wool-separated peripheral blood lymphocytes of Brucella-naive cattle and cattle immunized with B. abortus. Our results indicated that in cattle the lipopolysaccharide of B. abortus is not a B-cell mitogen. In immunized animals it stimulated predominantly nylon wool-adherent cells. The lipopolysaccharide of Escherichia coli O128:B12, in contrast, induced a substantially greater proliferative response in circulating lymphocytes, predominantly those adherent to nylon wool, of the Brucella-naive cattle.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects; D001428:Bacterial Vaccines / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000276:immunology; D014611:Vaccination
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D011135:Polysaccharides, Bacterial