Limited proteolysis of the porcine zona pellucida by homologous sperm acrosin.
Boar sperm acrosin isolated by affinity chromatography on p-(p'-aminophenoxypropoxy)benzamidine linked to Sepharose was tested for its proteolytic effect on the zona pellucida of freshly ovulated pig eggs. During 1 h in a physiological medium there was no observable change in the morphology of the zona pellucida but subsequent 125I labelling of the structure followed by electrophoretic analysis revealed that acrosin had asserted a limited and selective proteolysis.
D000176:Acrosin / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D008297:Male; D010063:Ovum / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism; D013552:Swine; D015044:Zona Pellucida / Q000187:drug effects / Q000378:metabolism*
D010455:Peptides; D010450:Endopeptidases; D000176:Acrosin