Immunoreactivity of vasopressin and a novel pituitary protein '7B2' in Long-Evans and Brattleboro rat hypothalamus and hypophysis.
The unlabeled antibody (peroxidase-anti-peroxidase) method was used to simultaneously localize vasopressin and a novel pituitary protein designated '7B2' in rat hypothalamus and pituitary. Results showed the common localization of both substances within magnocellular neurons of supraoptic, supraoptic retrochiasmic and paraventricular nuclei. The distribution was also similar in the inner zone of the median eminence and in the posterior lobe of the pituitary gland. Only 7B2 antiserum labeled the external zone of the median eminence and the intermediate and anterior lobes of the pituitary. In the Brattleboro rat the anterior and intermediate lobes were strongly labeled with 7B2-IR and there was some 7B2-staining in the hypothalamus and the posterior lobe, but the intensity of the reaction was diminished.
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D008473:Median Eminence / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D011910:Rats, Brattleboro; D013045:Species Specificity; D013495:Supraoptic Nucleus / Q000378:metabolism
D009419:Nerve Tissue Proteins; D001127:Arginine Vasopressin