Structure of the amino acid-containing capsular polysaccharide (K54 antigen) from Escherichia coli O6:K54:H10.
The structure of the K54-antigenic polysaccharide (K54 antigen) of Escherichia coli O6:K54:H10 was elucidated by determination of the composition, 1H- and 13C-n.m.r. spectroscopy, periodate oxidation, and a study of the oligosaccharides obtained by partial hydrolysis with acid. The K54 polysaccharide consists of----3)-beta-D-glucosyluronic acid-(1----3)-alpha-L-rhamnosyl-(1----repeating-units. Of the glucuronic acid residues, approximately 85% are substituted in the ratio 9:1 with L-threonine and L-serine amidically linked to the carboxyl group. The K54 polysaccharide has a molecular weight of approximately 160,000, corresponding to approximately 380 repeating-units.
D000596:Amino Acids / Q000032:analysis; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D002241:Carbohydrates / Q000032:analysis; D004926:Escherichia coli / Q000276:immunology*; D007122:Immunoelectrophoresis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000032:analysis
D000596:Amino Acids; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D002241:Carbohydrates; C030659:K antigens; D009844:Oligosaccharides