[Effect of proteolytic enzymes on the enhanced sensitivity of a cell culture of goat embryonic kidney to Newcastle disease virus].
The influence of proteolytic enzymes on the increase of the sensitivity of cell cultures to Newcastle disease virus was tested in the continuous culture of goat embryo kidney cells. In this investigation the dose of trypsin and the time of incubation for the treatment of the cell culture was selected. The preliminary treatment of the monolayer culture of goat embryo kidney cells with trypsin by incubation at a temperature of 18-20 degrees C for 30 minutes made it possible to increase the sensitivity of the culture to Newcastle disease virus 10-fold.
D000818:Animals; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D006041:Goats; D007668:Kidney / Q000187:drug effects* / Q000382:microbiology; D009522:Newcastle disease virus / Q000472:pathogenicity*; D010447:Peptide Hydrolases / Q000494:pharmacology*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D014776:Virus Cultivation / Q000379:methods
D010447:Peptide Hydrolases; D014357:Trypsin
Vliianie proteoliticheskikh fermentov na povyshenie chuvstvitel'nosti kul'tury kletok pochki émbriona kozy k virusu bolezni N'iukasla.