Synthesis and secretion of an epidermal growth factor (EGF) by human fibroblast cells in culture.
Human fibroblast (WS-1) cells in culture synthesized and secreted an epidermal growth factor which cross-reacted with human epidermal growth factor (hEGF) purified from human urine. hEGF secreted by the cells (designated as WS-1 EGF or fibroblast EGF) and hEGF isolated from urine (designated as urine EGF) were immunologically indistinguishable. The molecular weight of fibroblast EGF estimated by gel filtration was identical with that of hEGF from urine. On chromatofocusing chromatography, fibroblast EGF was eluted mainly at pH 4.26 as a sharp symmetric peak with a minor peak at pH 4.62, like urine EGF. These results suggested that EGF synthesized and secreted by human fibroblast cells is an identical molecule to that of hEGF in human urine.
D002478:Cells, Cultured; D002845:Chromatography / Q000379:methods; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000378:metabolism / Q000652:urine; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing; D008970:Molecular Weight
D004815:Epidermal Growth Factor