Further studies on the subcellular distribution of Cd2+ in Cd-sensitive and Cd-resistant strains of Saccharomyces cerevisiae.
When a Cd-resistant strain (301 N) and a Cd-sensitive strain (101 N) of Saccharomyces cerevisiae were incubated in medium containing Cd2+, a large proportion of the cellular Cd2+ was found in the cytosol of strain 301 N, but not in that of strain 101 N. Approximately 65% of the cellular Cd2+ was released from strain 301 N after treatment with chitosan, which affects cell membrane permeability. About 80% of the cellular Cd2+ released from strain 301 N by chitosan treatment was detected in a 30 000-10 000 molecular weight fraction prepared by ultrafiltration. The distribution of Cd2+ into the cytosol in strain 301 N was inhibited in the presence of cycloheximide. The proportion of cellular Cu2+ or Zn2+ present in the cytosol after incubation with these ions was similar for the two strains (about 40%).
D002104:Cadmium / Q000378:metabolism*; D002686:Chitin / Q000031:analogs & derivatives / Q000494:pharmacology; D048271:Chitosan; D003300:Copper / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013347:Subcellular Fractions / Q000032:analysis; D014462:Ultrafiltration; D015032:Zinc / Q000378:metabolism
C004203:cadmium-binding protein; D002104:Cadmium; D002686:Chitin; D003300:Copper; D048271:Chitosan; D008668:Metallothionein; D003513:Cycloheximide; D008274:Magnesium; D015032:Zinc