Effects of DNA sequence and conformation on nucleosome formation.
A simple theoretical analysis of the free energy balance controlling nucleosome formation shows that the specific effects of different DNA sequences and/or conformations observed in vitro are mainly due to their different elastic properties. A calculation of the elastic free energy required to fold DNA on histone octamers yields quantitative results rationalizing the experimental findings provided that: (i) the average helical repeat of DNA on nucleosomes is greater than 10.2 bp per turn, and (ii) poly[dG.dC] adopts an A-type conformation.
D001483:Base Sequence; D004247:DNA; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D009707:Nucleosomes; D011067:Poly dA-dT; D011089:Polydeoxyribonucleotides; D013816:Thermodynamics
D009707:Nucleosomes; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; C019530:poly(dC-dG); D004247:DNA