Comparison of a coagglutination test with the GM1-ELISA and the Y-1 adrenal cell assay for the detection of heat-labile enterotoxin from Escherichia coli.
A number of different assay methods has been developed for the detection of the heat-labile enterotoxin (LT) of ETEC strains isolated from humans and animals. In the present study 40 Escherichia coli strains were subject of a comparative study of the staphylococcal coagglutination (Coa-LT) test, the GM1-ELISA and the Y-1 adrenal cell test. All but two of 20 "LT-ST" or "LT-only" producing ETEC strains gave identical results in both the Coa-LT test and the bioassay. None of the 14 "ST only" producing ETEC strains gave false positive results. It is concluded that the Coa-LT test is a simple and rapid assay method for the routine diagnosis of LT producing ETEC strains.
D000311:Adrenal Glands; D000372:Agglutination Tests; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004768:Enterotoxins / Q000032:analysis* / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000032:analysis; D029968:Escherichia coli Proteins; D005677:G(M1) Ganglioside; D006801:Humans; D013045:Species Specificity
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D005677:G(M1) Ganglioside; C027538:heat-labile enterotoxin, E coli