Suppression of phorbol myristate acetate-triggering of macrophage H2O2 release by sarcoma 180 originating factor.
A high molecular weight proteinaceous factor in the cell extract of sarcoma 180 (S-180) was found to inhibit phorbol myristate acetate (PMA)-triggering of macrophage H2O2 release. This factor (S-180 factor) was stable at 56 C for 1 hr and resistant to ultraviolet-irradiation. The S-180 factor inhibited the specific binding of PMA to macrophages and this was accompanied by a parallel reduction of PMA-triggered H2O2 release. S-180 factor preferentially depressed macrophage H2O2 release in response to phorbol diesters including PMA, 4 beta-phorbol 12 13 beta,13 alpha-diacetate, 4 beta-phorbol 12 beta,13 alpha-didecanoate, 4 beta-phorbol 12 beta,13 alpha-dibenzoate, and 4-omicron-methyl-PMA rather than the H2O2 release triggered by wheat germ agglutinin or by phagocytosis of latex particles. The S-180 factor failed to affect the PMA-elicited macrophage cell spreading and macrophage phagocytic activity against latex beads with or without PMA-mediated stimulation. A similar inhibitory factor was found in the extracts of some other murine tumor cells (Ehrlich carcinoma and thymic leukemia) and normal cells (liver, spleen, and peritoneal exudate cells).
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002457:Cell Extracts / Q000494:pharmacology; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007938:Leukemia / Q000378:metabolism; D008262:Macrophage Activation; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D009363:Neoplasm Proteins / Q000494:pharmacology*; D010587:Phagocytosis; D012510:Sarcoma 180 / Q000378:metabolism; D013601:T-Lymphocytes; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002457:Cell Extracts; D008263:Macrophage Migration-Inhibitory Factors; D009363:Neoplasm Proteins; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate