Growth factor production during multistage transformation of epithelium in vitro. I. Partial purification and characterisation of the factor(s) from a fully transformed epithelial cell line.
Transforming growth factor (TGF)-like activity is characterised from one of a series of salivary epithelial cell lines, CSG 211, chemically transformed in vitro. In this transformation system, we can demonstrate multiple stages in the acquisition of a malignant phenotype by normal diploid ductal epithelial cells from male mouse submandibular gland. The fully transformed, tumorigenic cell TGF-like activity in serum-free supernatants resembles no other well-characterised growth factor and has an apparent molecular weight (Mr) of 14 kd. There is also evidence of a higher Mr activity, which is separable by anion exchange chromatography. We show that the premalignant, nontumorigenic progenitor cells of this line do not produce demonstrable TGF-like activity and that this property is therefore acquired as CSG 211 cells become carcinoma producing.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004261:DNA Replication / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D037102:Lectins / Q000378:metabolism; D008297:Male; D051379:Mice; D008858:Microscopy, Phase-Contrast; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D037121:Plant Lectins; D011817:Rabbits; D051381:Rats; D012469:Salivary Glands / Q000166:cytology*; D013997:Time Factors; D015291:Transforming Growth Factors
D037102:Lectins; D037121:Plant Lectins; C033619:lentil lectin; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors