Enteroadhesion fimbriae and enterotoxin of Escherichia coli: genetic transfer to a streptomycin-resistant mutant of the galE oral-route live-vaccine Salmonella typhi Ty21a.
An enterotoxigenic Escherichia coli plasmid encoding colonization factor antigen (CFA) I fimbriae and heat-stable toxin was transferred into a streptomycin-resistant mutant of the Salmonella typhi galE strain Ty21a (a live attenuated oral typhoid vaccine). The virulence plasmid-carrying transconjugants produced CFA I fimbriae and heat-stable toxin. The marked production of CFA I fimbriae was observed even in a vaccine medium for Ty21a. The data lead to a new type of potential live oral vaccine, S. typhi Ty21a producing enteroadhesion fimbriae.
D000942:Antigens, Bacterial / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D001428:Bacterial Vaccines; D002238:Carbohydrate Epimerases / Q000235:genetics*; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D009154:Mutation; D010957:Plasmids; D012485:Salmonella typhi / Q000235:genetics* / Q000276:immunology; D013307:Streptomycin / Q000494:pharmacology*; D014534:UDPglucose 4-Epimerase / Q000235:genetics*
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C022924:colonization factor antigens; C034151:heat stable toxin (E coli); D036781:Fimbriae Proteins; D002238:Carbohydrate Epimerases; D014534:UDPglucose 4-Epimerase; D013307:Streptomycin