Expression of an Escherichia coli flagellin gene, hag48, in the presence of a Salmonella H1-repressor.
An Escherichia coli K12 flagellin gene, hag48, was found to be expressed in the presence of the Salmonella rh1 gene product. The strains which had hag48 on a chromosome or on an F' factor were constructed from strains H2-e,n,xon-off rh1+ and fixed H2-e,n,xon rh1+ in which rh1+ is cotranscribed with H2 in its "on" state. Motility of these strains in semisolid medium was inhibited by anti-H48 serum and motile clones (swarms) that escaped from it were hag mutants in case of the hag48 e,n,xon-off strain tested. H48 flagellin was detected by electrophoresis, though its amount was less than e,n,x flagellin, from all the strains that were nonmotile in the presence of anti-H48 serum.
D001426:Bacterial Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005408:Flagellin / Q000235:genetics* / Q000302:isolation & purification; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000378:metabolism*; D012475:Salmonella / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*
D001426:Bacterial Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D005408:Flagellin