Liquid-chromatographic study of fluorescent compounds in hemodialysate solutions.
We have separated and identified three endogenous, naturally fluorescent substances in uremic hemodialysate by using reversed-phase liquid chromatography with fluorescence detection. Co-chromatography with authentic standards, monitoring peak shifts after enzymic treatment, and spectrofluorescence measurements were used to confirm the identity of indican, tryptophan, and indole-3-acetic acid. Concentrations of indican were about 1.5 those of tryptophan and considerably greater than those of indole-3-acetic acid in hemodialysate samples from 12 renal patients. These three compounds, as well as eight unidentified components, were consistently present in dialysate samples from each of the 12 patients.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007200:Indican / Q000032:analysis; D007210:Indoleacetic Acids / Q000032:analysis; D006435:Renal Dialysis; D012996:Solutions / Q000032:analysis; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000032:analysis; D014368:Tryptophanase; D014511:Uremia / Q000378:metabolism*
D007210:Indoleacetic Acids; D012996:Solutions; C030737:indoleacetic acid; D014364:Tryptophan; D014368:Tryptophanase; D007200:Indican