Assessment of the role of an immunofluorescent terminal deoxynucleotidyl transferase kit.
The laboratory use of a commercial kit for the immunofluorescent measurement of terminal deoxynucleotidyl transferase (BRL Terminal Deoxynucleotidyl Transferase Immunofluorescent Assay System) has been investigated. The precision of the assay system with stored patient material and its performance with regard to ease of microscopic examination were assessed. The intensity of the fluorescence obtained was found to vary. However, the precision of the assay system on fixed slides stored at -30 degrees C was satisfactory; the method simple, and results obtainable in a relatively short space of time.
D001853:Bone Marrow / Q000201:enzymology; D004796:Clinical Enzyme Tests; D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D004254:DNA Nucleotidyltransferases / Q000032:analysis*; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007938:Leukemia / Q000145:classification / Q000175:diagnosis*; D007945:Leukemia, Lymphoid / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D011933:Reagent Kits, Diagnostic; D013048:Specimen Handling
D011933:Reagent Kits, Diagnostic; D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase