Antibiotic monitoring in body fluids.
Analytical procedures recently described for the quantitative determination of antibiotics in body fluids are reviewed. High-performance liquid chromatography (HPLC) and immunoassays appear as an alternative to current microbiological assays. HPLC has been applied to most antibiotics in clinical use and a major part of the review deals with this technique. Attention is given to sample pretreatment, characteristics of chromatography and detection, and limit of sensitivity. Non-isotopic immunoassays have been essentially applied to aminoglycosides and vancomycin and are also reviewed. Advantages and drawbacks of HPLC and immunoassays are presented.
D000617:Aminoglycosides / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001398:Aztreonam; D001681:Biological Assay; D001826:Body Fluids / Q000032:analysis; D002511:Cephalosporins / Q000032:analysis; D002701:Chloramphenicol / Q000032:analysis; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006801:Humans; D007118:Immunoassay; D008991:Monitoring, Physiologic; D010406:Penicillins / Q000097:blood; D012293:Rifampin / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013839:Thiamphenicol / Q000032:analysis; D014640:Vancomycin / Q000032:analysis
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D010406:Penicillins; D002701:Chloramphenicol; D014640:Vancomycin; D013839:Thiamphenicol; D001398:Aztreonam; D012293:Rifampin