The proteolytic activities of chymopapain, papain, and papaya proteinase III.
The three proteinases present in papaya latex: papain (EC 3.4.22.2) chymopapain and papaya proteinase III (EC 3.4.22.6), were standardized by active-site titration, and compared in proteolytic activity against azocasein, serum albumin and cartilage proteoglycan. The activities were all of the same order, although there were differences in pH dependence. SDS-polyacrylamide gel electrophoresis of the early products of digestion of albumin and phosphorylase a showed very similar patterns for the three papaya proteinases. Kinetic parameters for hydrolysis of benzyloxycarbonyl-phenylalanyl-arginyl-7(4-methyl)coumarylamide were determined for the three enzymes. Values for kcat/Km varied only within a factor of 2, but the individual constants were much higher for papain than for chymopapain and papaya proteinase III. In contrast to the results obtained with the synthetic substrate, the kinetic parameters for the initial hydrolysis of succinyl-albumin were very similar for the three papaya proteinases. This was consistent with their similar proteolytic activities in other assays.
D000418:Albumins / Q000378:metabolism; D001665:Binding Sites; D002356:Cartilage / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D002917:Chymopapain / Q000378:metabolism*; D003374:Coumarins / Q000378:metabolism; D004151:Dipeptides; D004586:Electrophoresis; D010450:Endopeptidases / Q000378:metabolism*; D005779:Immunodiffusion; D007700:Kinetics; D010206:Papain / Q000378:metabolism*; D010762:Phosphorylase a / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D012709:Serum Albumin; D012710:Serum Albumin, Bovine / Q000378:metabolism
D000418:Albumins; D002364:Caseins; D003374:Coumarins; D004151:Dipeptides; D011509:Proteoglycans; D012709:Serum Albumin; C026018:azocasein; C034877:succinylalbumin; D012710:Serum Albumin, Bovine; C021159:benzyloxycarbonyl-phenylalanylarginine-4-methylcoumaryl-7-amide; D010762:Phosphorylase a; D010450:Endopeptidases; D010206:Papain; D002917:Chymopapain