Experimental study of fetal liver transplantation.
Fetal liver cells function as liver and bone marrow cells. The direct and indirect effects of fetal liver cell transplants on liver and/or bone marrow failure was investigated in rat experiments. Outbred Wistar, inbred Lewis and F344 fetus rats (18-20 days) were used as donors; Wistar and Lewis rats (210-280g) were used as recipients. D-galactosamine (1.0-1.85g/kg) was administered intraperitoneally inducing acute liver failure. TBI, Co60 1000 Rads, was given inducing bone marrow failure. Donor liver cells were prepared using the following four methods: 1) simple liver homogenation; 2) centrifugation by percol gradient into different fractions; 3) arginine deficient culture; and 4) pronase culture. Preliminary observations of the transplant effect of fetal liver cells on liver and/or bone marrow failure is described.
D000208:Acute Disease; D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001855:Bone Marrow Diseases / Q000628:therapy; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured / Q000502:physiology; D002499:Centrifugation, Density Gradient; D056486:Chemical and Drug Induced Liver Injury; D005333:Fetus; D008099:Liver / Q000166:cytology / Q000196:embryology; D008107:Liver Diseases / Q000628:therapy; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D014185:Transplantation, Isogeneic / Q000401:mortality