A highly sensitive enzyme immunoassay (EIA) system for mouse epidermal growth factor (mEGF).
A highly sensitive two-site enzyme immunoassay system for mouse epidermal growth factor (mEGF) was developed, based on the sandwiching of an antigen between anti-mouse EGF IgG antibody-coated on a polystyrene bead and anti-mouse EGF Fab' antibody-linked peroxidase (horseradish peroxidase, EC. 1.11.1.7). The procedure is simple and rapid compared to a bioassay. Also, the Fab' antibody-peroxidase complex is more stable than the 125I-labeled antibody. Purified mEGF is detectable at a concentration as low as 3 pg/ml. The detection range was 0.3 to 680 pg/sample with 0.1 ml samples. Levels of immunoreactive mEGF in extracts from adult male mice well agreed with those determined by a radioimmunoassay and a radioreceptor assay. The submaxillary gland contained an extremely high concentration of EGF, while other tissues had low levels of EGF.
D000818:Animals; D004815:Epidermal Growth Factor / Q000032:analysis*; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D051379:Mice; D008832:Microchemistry; D013363:Submandibular Gland / Q000032:analysis; D014018:Tissue Distribution
D007106:Immune Sera; D007202:Indicators and Reagents; D004815:Epidermal Growth Factor