The use of anti-synthetic peptide antibodies to study the v-erb B protein from chicken and rat cells transformed by avian erythroblastosis virus.
Two site-specific anti-peptide antisera have been produced that efficiently recognize the native form of the v-erb B protein from avian erythroblastosis virus (AEV)-infected chicken erythroblasts and fibroblasts, and from AEV-transformed mammalian cells. Since the antibodies were generated against synthetic sequences, the immunoprecipitations could be performed in the presence or absence of immunizing peptide, permitting the specifically precipitated proteins to be identified from background non-specifically adsorbed proteins. We confirmed that immobilized v-erb B protein from cell lysates of unlabelled AEV-infected chicken erythroblasts became labelled upon incubation with [gamma-32P]ATP. In addition we demonstrated for the first time that v-erb B from mammalian cells became labelled under the same conditions. These results suggest that the v-erb B protein may possess intrinsic kinase activity. The reagents described should permit further investigations as to whether this activity plays a role in maintaining cellular transformation.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001355:Alpharetrovirus; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002472:Cell Transformation, Viral; D002645:Chickens; D004900:Erythroblasts; D005347:Fibroblasts; D010455:Peptides / Q000276:immunology*; D010761:Phosphorus Radioisotopes; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D014764:Viral Proteins
D000906:Antibodies; D010455:Peptides; D010761:Phosphorus Radioisotopes; D010982:Platelet-Derived Growth Factor; D014764:Viral Proteins; D000255:Adenosine Triphosphate; D011494:Protein Kinases