Isolation and characterization of fatty acid-binding protein from rat brain.
A fatty acid-binding protein has been identified and isolated from the cytosol fraction of rat brain. The fatty acid-binding protein was purified to homogeneity by gel filtration and preparative isoelectric focusing. The binding protein was different from Z protein from rat liver in its isoelectric point and immunological reactivity, in spite of its similar molecular weight of 12,000. Rabbit antibodies against rat liver Z protein were used to demonstrate that the fatty acid-binding proteins from rat liver and brain are immunologically unrelated, and that no Z protein is present in rat brain cytosol.
D000818:Animals; D001667:Binding, Competitive; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003600:Cytosol / Q000032:analysis; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008099:Liver / Q000032:analysis; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D011919:Rats, Inbred Strains
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins