Monoclonal antibody to human lysosomal alpha-glucosidase in immunocytochemistry: unexpected reactivity with cytoskeletal structures.
A well-characterized monoclonal antibody against human lysosomal alpha-glucosidase has been used for the immunohistochemical localization of the enzyme in cultured human skin fibroblasts. Under conditions that are routinely used for the preparation of cells for immunocytochemistry, this monoclonal antibody does not react with acid alpha-glucosidase but in contrast with components of the cytoskeleton. Double-labelling experiments with the monoclonal antibody and rabbit anti-vimentin antiserum identified the cytoskeletal components as intermediate filaments. The implications of this observation for the use of monoclonal antibodies in immunocytochemistry in general are discussed.
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000201:enzymology* / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D005959:Glucosidases / Q000378:metabolism*; D006008:Glycogen Storage Disease / Q000201:enzymology; D006651:Histocytochemistry; D006801:Humans; D007223:Infant; D008247:Lysosomes / Q000201:enzymology* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D012867:Skin / Q000201:enzymology*; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005959:Glucosidases; D000520:alpha-Glucosidases