[Immunomorphological study of 1,2-dimethylhydrazine-induced carcinomas of the large intestine in rats using monoclonal antibodies to intermediate filament proteins].
Cryostatic sections of rat large bowel tumors induced by 1,2-dimethylhydrazine were stained with monoclonal antibodies against different proteins of intermediate filaments: (a) against prekeratin (mol. mass 49 000, PK49) found in many epithelial cells and (b) against vimentin, a constituent of intermediate filaments of mesenchymal cells. Immunofluorescence study showed that large bowel tumor cells as well as normal cells of this organ contain PK49 but not vimentin. High sensitivity of the method allowed one to clearly identify small invasive nodules and groups of tumor cells not visible in usual histologic preparations. Moreover, in some cases single atypical tumor cells were identified in tumor stroma and in the submucosal layer underlying the tumor, that were indistinguishable from normal mesenchymal cells at the light microscopy level.
D019813:1,2-Dimethylhydrazine; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D004127:Dimethylhydrazines; D005455:Fluorescent Antibody Technique; D006652:Histological Techniques; D007381:Intermediate Filament Proteins / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D007414:Intestinal Neoplasms / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D007420:Intestine, Large / Q000276:immunology* / Q000473:pathology; D008297:Male; D051381:Rats
D000911:Antibodies, Monoclonal; D004127:Dimethylhydrazines; D007381:Intermediate Filament Proteins; D019813:1,2-Dimethylhydrazine
Immunomorfologicheskoe izuchenie kartsinom tolstoĭ kishki krys, indutsirovannykh 1,2-dimetilgidrazinom, s ispol'zovaniem monoklonal'nykh antitel k belkam promezhutochnykh filamentov.