Glutaraldehyde preparation of coronary artery bypass bioprostheses.
A method for glutaraldehyde (GA) fixation of canine carotid arteries has been developed for the preparation of small caliber biologic prostheses for coronary artery bypass. The biologic grafts were preserved by a static inflation technique that proved to be more advantageous than the standard stenting method. The most suitable static inflation pressure was found to be 120 mm Hg. By means of colorimetric measurements the minimal tanning time and the amount of GA required for complete fixation for canine vascular tissue were established. Stabilization of the vessel collagen and confirmation of GA-collagen cross-linking were verified by evaluation of the elastic properties and shrinkage temperature of the grafts. Stress-strain measurements were evaluated to determine the number of cross-links introduced in the vascular tissue by GA. This number was shown to be proportional to the inflation pressure. Ethyl alcohol was chosen as the storage solution because it maintained the best physical, chemical, and histologic characteristics of the grafts. Biological evaluations were performed with carotid implants that were examined following acute low flow studies and implantations up to 112 days. All implantations have yielded 100% patencies.
D000447:Aldehydes; D000818:Animals; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries; D003094:Collagen / Q000032:analysis; D001026:Coronary Artery Bypass; D004285:Dogs; D004548:Elasticity; D005069:Evaluation Studies as Topic; D005976:Glutaral; D014021:Tissue Preservation
D000447:Aldehydes; D003094:Collagen; D005976:Glutaral