Diphtheria toxin promoter function in Corynebacterium diphtheriae and Escherichia coli.
The expression of the diphtheria tox228 gene encoding the nontoxic, serologically related CRM228 mutant diphtheria toxin has been analyzed in Corynebacterium diphtheriae and Escherichia coli. The diphtheria toxin promoter has been used to direct the expression of beta-galactosidase in E.coli, and the efficiency of promotion has been compared to that obtained with the lac promoter. Expression in C.diphtheriae is known to be dependent on the absence of iron, and we present for the first time direct evidence that this regulation occurs at the level of transcription. The 5' end of toxin mRNA maps at the same position in C.diphtheriae and E.coli, suggesting identical sequences to be recognized by C.diphtheriae and E.coli RNA polymerase. The diphtheria toxin promoter carries at position -34 a TTGATT sequence closely related to the E.coli -35 consensus sequence and in the -14 to -8 region a set of overlapping sequences with complete or partial homology to the E.coli -10 consensus sequence.
D001483:Base Sequence; D003353:Corynebacterium diphtheriae / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004167:Diphtheria Toxin / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D007763:Lac Operon; D009154:Mutation; D009876:Operon; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
D004167:Diphtheria Toxin; D012333:RNA, Messenger; D012321:DNA-Directed RNA Polymerases