Hypermutation at the immunoglobulin heavy chain locus in a pre-B-cell line.
Most cells in the well-known pre-B-lymphocyte line 18-81 have correctly assembled genes for both alleles at the immunoglobulin heavy chain locus. Only one allele is "active"; the other, "silent" allele contains an amber termination codon. The rate of reversion of this amber codon was determined to be 0.3-1 X 10(-4) per cell generation. This high rate is termed hypermutation.
D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002451:Cell Compartmentation; D003062:Codon / Q000032:analysis; D005786:Gene Expression Regulation; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008433:Mathematics; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000235:genetics; D009154:Mutation; D013234:Stem Cells / Q000276:immunology*
D003062:Codon; D007143:Immunoglobulin Heavy Chains