Monoclonal antibodies that distinguish between human aorta smooth muscle and endothelial cells.
A monoclonal antibody has been generated that interacts with the surface of cultured human aorta smooth muscle cells and does not bind to the endothelial cells from aorta and umbilical vein. An antigen recognized by the antibody has a molecular mass of 330 kDa as determined by electrophoresis of immunoprecipitate in SDS-polyacrylamide gel. The same antigen appeared to be present on the fibroblast surface while neither immunofluorescence, flow cytofluorimetry nor immunoprecipitation reveal it on the endothelial cell surface or in the Triton X-100 extract.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004727:Endothelium / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000276:immunology*; D017830:Octoxynol; D011092:Polyethylene Glycols
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011092:Polyethylene Glycols; D017830:Octoxynol