Comparative studies on surface antigenicity of Trypanosoma cruzi trypomastigotes from infected mouse blood and infected L-cell cultures.
Differences in the antigenicities of surface components of blood-form trypomastigotes and trypomastigotes derived from L-cell cultures were studied by agglutination and indirect immunofluorescent tests on living parasites using various antisera from rabbits and mice. Antisera from rabbits immunized with L-cell-derived trypomastigotes and antisera obtained from rabbits infected with L-cell-derived trypomastigotes showed similar titers in both the agglutination and immunofluorescent test. Moreover, both antisera exhibited higher titers against trypomastigotes derived from L-cell cultures than against blood-form trypomastigotes. No detectable agglutination titer against either blood-form or L-cell-derived trypomastigotes was observed with sera from (a) mice infected with blood-form trypomastigotes after previous immunization with blood-form trypomastigotes, (b) mice infected with blood-form trypomastigotes and then treated with Lampit, or (c) mice infected with slightly less virulent trypomastigotes from L-cells. However, detectable and almost equal titers were observed with sera from (a), (b) and (c) in indirect immunofluorescent tests. Mouse sera also exhibited higher titers against trypomastigotes derived from L-cells than against the blood-form type. However, mouse sera showed more pronounced differences than rabbit sera. These results suggest that there may be two types of trypomastigotes in infected animals and that the surface components of blood-form trypomastigotes have lower antigenicity.
D000372:Agglutination Tests; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis*; D001769:Blood / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D007739:L Cells / Q000469:parasitology*; D051379:Mice; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000276:immunology*
D000953:Antigens, Protozoan; D000954:Antigens, Surface