Binding of fibronectin to DNA: new application of the Crithidia luciliae immunofluorescence test.
Binding of purified plasma fibronection to Crithidia luciliae kinetoplast DNA was demonstrated by fluorescence microscopy. The method was suitable for the detection of fibronectin in human plasma diluted 1:256 and 1 microgram/ml concentration of isolated fibronectin. Purified human Clq, monoclonal human myeloma proteins of IgG1 and IgG3 subclasses and calf thymus DNA inhibited the binding of fibronectin to kinetoplasts. The method can be used as a functional assay for fibronectin and for various materials containing fibronectin.
D000818:Animals; D000936:Antigen-Antibody Complex; D003421:Crithidia / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005353:Fibronectins / Q000097:blood / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007122:Immunoelectrophoresis; D007136:Immunoglobulins; D011485:Protein Binding
D000936:Antigen-Antibody Complex; D005353:Fibronectins; D007136:Immunoglobulins; D004247:DNA