Determination of choline-containing phospholipids in human bile and serum by a new enzyme sensor.
A simple method for direct determination both of choline and lecithin (phosphatidylcholine) in human bile and blood sera was developed. An enzyme electrode, based on immobilized choline oxidase on nylon net and an oxygen Clark electrode was assembled. Phosphatidylcholine can be determined by use of phospholipase D as hydrolyzing agent. Reliable results were obtained in the case of determination of lecithin and choline in bile samples.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001646:Bile / Q000032:analysis*; D002794:Choline / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D004566:Electrodes; D004800:Enzymes, Immobilized; D006801:Humans; D010713:Phosphatidylcholines / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D013053:Spectrophotometry
D004800:Enzymes, Immobilized; D010713:Phosphatidylcholines; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D010739:Phospholipase D; D002794:Choline