The base sequence of the nifF gene of Klebsiella pneumoniae and homology of the predicted amino acid sequence of its protein product to other flavodoxins.
The nucleotide sequence of a 629 base-pair segment of DNA spanning the nifF gene of Klebsiella pneumoniae is presented. The structural gene comprises 531 base-pairs (175 codons, excluding the translational initiator and terminator) encoding an acidic polypeptide of 18950 Da. The nifF product thus belongs to the long-chain class of flavodoxins. It shows some sequence homology to the short-chain flavodoxins from Desulfovibrio vulgaris, Clostridium MP and Megasphaera elsdenii, and much stronger homology to long-chain flavodoxins from Azotobacter vinelandii and Anacystis nidulans. The long chain flavodoxins thus seem to constitute a well-conserved sub-group. The homology with the A. vinelandii flavodoxin is particularly strong, which may reflect their common function in nitrogen fixation.
D000595:Amino Acid Sequence; D001483:Base Sequence; D005418:Flavodoxin / Q000235:genetics*; D005420:Flavoproteins / Q000235:genetics*; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics*; D014176:Protein Biosynthesis; D013045:Species Specificity
D005418:Flavodoxin; D005420:Flavoproteins