DNA-directed in vitro synthesis and assembly of the form II D-ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides.
A biochemical analysis of the in vitro assembly of the form II ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides after transcription and translation from cloned DNA is presented. The predominant enzymatically active oligomeric forms of the in vitro-synthesized and -assembled ribulose-1,5-bisphosphate carboxylase are tetramers and hexamers. Assembly of the monomeric subunits to form active enzyme appears to be dependent on the presence of a minimum number of subunits in the cell extract. Assembly of ribulose-1,5-bisphosphate carboxylase also was observed when the protein-synthesizing extracts were prepared from cells which were partially derepressed for ribulose-1,5-bisphosphate carboxylase expression.
D004269:DNA, Bacterial / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D010105:Oxygenases / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000235:genetics*; D012247:Rhodospirillum rubrum / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D046911:Macromolecular Substances; D010105:Oxygenases; D012273:Ribulose-Bisphosphate Carboxylase