Differential effects of 7,8-benzoflavone on polycyclic aromatic hydrocarbon dependent mutagenesis and cytotoxicity in a keratinocyte cell-mediated mutation assay.
A murine keratinocyte cell-mediated mutagenesis assay was used to examine the effects of 7,8-benzoflavone (7,8-BF) on the metabolic activation of 7,12-dimethylbenz[a]anthracene (DMBA) and benzo[a]pyrene (BP) to mutagenic and cytotoxic metabolites. DMBA-dependent mutagenesis and cytotoxicity were strongly and coordinately inhibited by 7,8-BF in a concentration-dependent manner. BP-dependent mutagenesis was also strongly inhibited by 7,8-BF in a concentration-dependent manner; whereas BP-dependent cytotoxicity was relatively unaffected by 7,8-BF. Maximum inhibition of DMBA- and BP-dependent mutagenesis occurred when the cultured keratinocytes were treated with 7,8-BF immediately prior to the addition of BP or DMBA.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism*; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001571:Benzoflavones / Q000494:pharmacology*; D001711:Biotransformation; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004817:Epidermis / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology*; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*
D001571:Benzoflavones; D005419:Flavonoids; D009153:Mutagens; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; C011512:alpha-naphthoflavone