The effect of NaCl intake on 9-ketoprostaglandin reductase activity in the rabbit kidney.
Renal 9-ketoprostaglandin reductase activity from rabbits fed 0.3 g or 2.5 g NaCl per 100 g chow was measured in both centrifuged homogenates and in purified enzyme fractions. There was no salt related increase in 9-ketoprostaglandin reductase activity. PGA1-glutathione, 9, 10-phenanthrenequinone, and 4-nitrobenzaldehyde were better substrates for the purified 9-ketoprostaglandin reductases than was PGE2. Several carbonyl reductases were isolated which used PGA1-glutathione, 9, 10-phenanthrenequinone, and 4-nitrobenzaldehyde, but not PGE2, as substrates. Although PGA1-glutathione was a more faithful indicator of PGE2-related 9-ketoprostaglandin reductase activity than either 9, 10-phenanthrenequione or 4-nitrobenzaldehyde, it did not always provide an accurate estimate of that activity.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D001547:Benzaldehydes / Q000378:metabolism; D015232:Dinoprostone; D011464:Epoprostenol / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D010616:Phenanthrenes / Q000378:metabolism; D011454:Prostaglandins A / Q000378:metabolism; D011456:Prostaglandins B / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D011817:Rabbits; D012965:Sodium Chloride / Q000494:pharmacology; D013379:Substrate Specificity; D014882:Water-Electrolyte Balance
D001547:Benzaldehydes; D010616:Phenanthrenes; D011454:Prostaglandins A; D011456:Prostaglandins B; D011458:Prostaglandins E; C034467:prostaglandin B1; C005399:9,10-phenanthrenequinone; D012965:Sodium Chloride; C029915:4-nitrobenzaldehyde; D011464:Epoprostenol; D000429:Alcohol Oxidoreductases; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; C035858:prostaglandin-E2 9-reductase; C019642:alcohol dehydrogenase (NADP+); D005978:Glutathione; D015232:Dinoprostone; C100573:prostaglandin A1