Large scale production of glucose oligomers and polymers for physiological studies in humans.
Methods for isolating relatively large quantities of glucose oligomer and polymer subfractions from a partial corn starch hydrolysate (PCSH) are described. To ensure that the products are suitable for physiological studies in humans, potentially toxic substances were excluded from the preparative processes. For long chain glucose polymer fractions with degrees of polymerization (DP) averaging 43 glucose units, we employed molecular filtration through Amicon YM5 membranes. For fractions containing glucose oligomers with DP's 3 through 8, we employed yeast fermentation followed by ethanol fractionation.
D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D000431:Ethanol; D005285:Fermentation; D005947:Glucose / Q000032:analysis*; D006801:Humans; D009844:Oligosaccharides / Q000032:analysis; D011108:Polymers; D011134:Polysaccharides / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D002241:Carbohydrates; D009844:Oligosaccharides; D011108:Polymers; D011134:Polysaccharides; D000431:Ethanol; D005947:Glucose