Furosemide-sensitive Na+-K+ cotransport and cellular metabolism in human erythrocytes.
Metabolic depletion of human red cells with 2-deoxy-D-glucose in the presence of EGTA decreased ATP to about 4% of the initial value and increased total ouabain- and furosemide-resistant Na+ and K+ effluxes by 20% and 100%, respectively, and furosemide-sensitive Na+ and K+ effluxes by 100% and 60%, respectively. When ATP was restored, all the components of Na+ and K+ fluxes measured returned to baseline levels suggesting a metabolic dependence.
D000255:Adenosine Triphosphate / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005665:Furosemide / Q000494:pharmacology*; D006801:Humans; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D004533:Egtazic Acid; D010042:Ouabain; D005665:Furosemide; D000255:Adenosine Triphosphate; D003847:Deoxyglucose; D012964:Sodium; D011188:Potassium