Mitogenic effects of phorbol myristate acetate (PMA) on amphibian cells.
Tumour promoter phorbol myristate acetate (PMA) stimulates and causes proliferation of cells from the spleen, thymus and peripheral blood of Xenopus laevis, the clawed toad. Phagocytic cells become activated and from foci of clustered cells. B cells blast and change membrane immunoglobulin (Ig) expression; a small portion go on to secrete Ig. The surface Ig-negative splenocyte population, which contains T cells, responds to PMA by active proliferation. Splenocytes, once stimulated by PMA, become refractory to further addition of PMA but respond instead to ConA. These results are discussed in the general context of the effect of PMA lymphocytes.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A; D004261:DNA Replication / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D010704:Phorbols / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013950:Thymus Gland / Q000276:immunology; D014981:Xenopus
D010704:Phorbols; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate