Structures of the carbohydrate moieties of two monoclonal human lambda-type immunoglobulin light chains.
Human Bence Jones proteins of lambda type, Wh and Nei, both of which belong to subgroup II, contain an asparagine-linked sugar chain. Their carbohydrate moieties were liberated as oligosaccharides by hydrazinolysis and labeled by reduction with NaB3H4 after N-acetylation. Structural studies of each oligosaccharide by sequential exoglycosidase digestion in combination with methylation analysis revealed that Wh lambda contains the mono- and disialylated oligosaccharides (Formula: see text). These oligosaccharides are different from the oligosaccharides found in another lambda-type Bence Jones protein, Sm lambda, by Chandrasekaran et al. [Chandrasekaran, E. V., Mendicino, A., Garver, F. A., & Mendicino, J. (1981) J. Biol. Chem. 256, 1549-1555] and Garver et al. [Garver, F. A., Chang, L. S., Kiefer, C. R., Mendicino, J., Chandrasekaran, E. V., Isobe, T., & Osserman, E. F. (1981) Eur. J. Biochem. 115, 643-652].
D000911:Antibodies, Monoclonal / Q000032:analysis*; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis*; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D009844:Oligosaccharides