A macrophage-derived factor that inhibits the production and action of interleukin 2.
The human monocytic leukemia cell line THP-1 produces an immunosuppressive factor that inhibits interleukin 1 (IL-1)-dependent proliferation of mouse thymocytes as well as the mitogenic effects of concanavalin A (Con A) and phytohemagglutinin (PHA) on human peripheral blood mononuclear cells. The mechanism of action of this factor includes interference with both the production of interleukin 2 (IL-2) and its effects on target cells. Thus, the suppressor abrogates the proliferation of an IL-2-dependent cytotoxic T cell line (CTLL), but not of IL-2 independent cells like the L929 fibroblasts or the EL4 T lymphoma and U937 histiocytic lymphoma lines. It also suppresses IL-2 production by human peripheral blood enriched T cells and mouse splenocytes. The mediator has a molecular weight of 60,000-70,000 dalton, as determined by gel filtration chromatography, is heat labile, and is sensitive to trypsin, chymotrypsin, and protease.
D000818:Animals; D002460:Cell Line; D006358:Hot Temperature; D006801:Humans; D007166:Immunosuppressive Agents / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D007375:Interleukin-1 / Q000037:antagonists & inhibitors; D007376:Interleukin-2 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D037102:Lectins / Q000037:antagonists & inhibitors; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D010447:Peptide Hydrolases; D011506:Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects
D007166:Immunosuppressive Agents; D007375:Interleukin-1; D007376:Interleukin-2; D037102:Lectins; D011506:Proteins; C046429:macrophage-derived immunosuppressor factor; D010447:Peptide Hydrolases