In vitro migration inhibition of [3H]leucine-labeled granulocytes by supernatants of Con A-Sepharose stimulated MNC cultures.
By measuring in vitro migration of [3H]leucine-labeled granulocytes from agarose containing capillary tube fragments, the migration inhibitory activity of supernatants of Con A-Sepharose stimulated MNC cultures was tested. The sensitivity of the method was increased by using higher dilutions of granulocytes in agarose (from 10(7)/1 microliter to 10(7)/3-4 microliter). Serum-free assays have been used for characterizing the migration inhibitory activity in this system. The migration inhibitory activity was abolished by absorption on granulocytes or by heating (56 degrees C, 80 degrees C). Other factors such as L-fucose, N-acetyl-D-glucosamine, DFP or BAEE showed only moderate or insignificant impairing effects on migration inhibitory activity of crude supernatants of Con A-Sepharose stimulated MNC cultures.
D000117:Acetylglucosamine / Q000494:pharmacology; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D005643:Fucose / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology*; D007158:Immunologic Techniques; D008222:Lymphokines / Q000502:physiology*; D009000:Monocytes / Q000166:cytology*; D011480:Protease Inhibitors / Q000494:pharmacology
D003470:Culture Media; D008222:Lymphokines; D011480:Protease Inhibitors; D003208:Concanavalin A; D005643:Fucose; D000117:Acetylglucosamine