Direct measurement of antigens in serum by time-resolved fluoroimmunoassay.
A long-lived fluorescence label (Tb3+) has been attached to the antigen of interest by using a bifunctional chelating agent 1-(p-benzenediazonium)-EDTA. A nonequilibrium competitive-binding immunoassay protocol, in conjunction with time-resolved detection of the long-lived fluorescence label, allows the antigen to be analyzed directly in samples containing diluted human serum. Results obtained for immunoglobulin G with this simple and rapid procedure correlated well (r = 0.93) with those by a commercially available fluorescence immunoassay method.
D000941:Antigens / Q000032:analysis*; D003432:Cross-Linking Reagents; D004492:Edetic Acid / Q000031:analogs & derivatives; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013725:Terbium; D013997:Time Factors
D000941:Antigens; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D013725:Terbium; C017826:azophenyl-EDTA; D004492:Edetic Acid