Altered DNA contacts made by a mutant AraC protein.
Mutant AraC proteins were selected for their ability to induce but not to repress, or their ability to repress but not to induce the araBAD operon. One such unusual mutant is able to bind to the araI site with an affinity only two to three-fold weaker than the wild type AraC protein, but the mutant protein was shown, both in crude extracts and when purified, to contact only two of the three major groove regions of the DNA that are contacted by the wild type protein.
D051777:AraC Transcription Factor; D001089:Arabinose / Q000235:genetics; D001426:Bacterial Proteins; D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005786:Gene Expression Regulation / Q000187:drug effects*; D006147:Guanine / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010710:Phosphates / Q000378:metabolism; D010957:Plasmids; D012097:Repressor Proteins / Q000494:pharmacology*; D014157:Transcription Factors / Q000494:pharmacology*
D051777:AraC Transcription Factor; C497371:AraC protein, E coli; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D010710:Phosphates; D012097:Repressor Proteins; D014157:Transcription Factors; D006147:Guanine; D001089:Arabinose