An abundant ubiquitous glycoprotein (GP100) in nucleated mammalian cells.
Two-dimensional gel electrophoresis with the 125I-Con A overlay and affinity purification with Con A-agarose revealed the presence of an abundant ubiquitous 100-kDa glycoprotein (GP100) in nucleated mammalin cells. The amount in cultured human and murine cells varies from 3 to 20 X 10(6) molecules per cell making GP100 the most abundant glycoprotein in nucleated cells. Peptide mapping shows that it is different from erythrocyte Band III protein. Several properties of GP100 suggest that it could play a structural role in nucleated cell membranes.
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology*; D002477:Cells / Q000032:analysis*; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006023:Glycoproteins / Q000032:analysis*; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D007526:Isoelectric Point; D012515:Mast-Cell Sarcoma / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000032:analysis
D001457:Anion Exchange Protein 1, Erythrocyte; D006023:Glycoproteins; D003208:Concanavalin A