The use of caged aeration for the growth of animal cells on microcarriers.
A simple, unaerated 0.1 litre microcarrier propagator was successfully sealed up to 1 litre using constant tip speed of the flexible wide blade stirrer. Further scale-up to 10 litres was not successful because of inadequate aeration. When this deficiency was corrected, using a sintered aerator, cell growth did not improve due to excessive foam production. This was overcome by carrying out the necessary aeration inside a special cage, located inside the propagator, which retained the bubbles and excluded the microcarriers. Using this device cell growth improved to levels similar to those obtained at the 0.1 and 1 litre scales.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D006224:Cricetinae; D003584:Cytological Techniques; D007668:Kidney; D008863:Microspheres; D010101:Oxygen Consumption