Optimization of the urinary acid alpha-glucosidase determination.
The optimization of the method for acid alpha-glucosidase determination (EC 3.2.1.3) in human urines, employing the synthetic substrate 4-nitrophenyl-alpha-D-glucopyranoside, is reported. Storage conditions of the specimens and their pretreatment were particularly investigated. The precision of the whole analytical procedure (including gel filtration) is good (within-run CV = 7.4% for normal samples and 3.7% for elevated ones). The correlation with the method using maltose as substrate is excellent (y = -0.01 + 0.13 x; r = 0.9893).
D002850:Chromatography, Gel; D004355:Drug Stability; D005959:Glucosidases / Q000652:urine*; D005960:Glucosides; D006801:Humans; D007202:Indicators and Reagents; D007676:Kidney Failure, Chronic / Q000201:enzymology / Q000652:urine; D016030:Kidney Transplantation; D007700:Kinetics; D012016:Reference Values; D013053:Spectrophotometry / Q000379:methods; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000652:urine*
D005960:Glucosides; D007202:Indicators and Reagents; C019502:4-nitrophenyl alpha-glucoside; D005959:Glucosidases; D000520:alpha-Glucosidases