Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis.
Hybridoma cells produced by fusing myeloma cells with spleen cells from mice immunized with a soluble antigen of Microsporum canis yielded 30 antibody-producing clones. Six of these clones, propagated as ascites tumors in mice, showed two different types of monoclonal antibodies. The type 1 monoclonal antibody reacted with 17 heterologous and 10 homologous dermatophyte antigens. Type 2 monoclonal antibodies were unable to precipitate three antigens from different isolates of M. canis, thus suggesting the occurrence of different serotypes within the species.
D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000946:Antigens, Fungal / Q000276:immunology; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D005779:Immunodiffusion; D051379:Mice; D008807:Mice, Inbred BALB C; D008864:Microsporum / Q000145:classification* / Q000276:immunology; D012703:Serotyping / Q000379:methods*; D013154:Spleen
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal