Platelet derived growth factor induces ornithine decarboxylase activity in NIH 3T3 cells.
Incubation with highly purified human Platelet Derived Growth Factor induced ornithine decarboxylase activity in quiescent NIH 3T3 cells concomitantly with mitogenic stimulation. Pretreatment of cells with a specific ornithine decarboxylase inhibitor, DL-alpha-difluoromethyl-ornithine significantly inhibited the effect of the mitogen on DNA synthesis. These experiments suggest that the mitogenic activity of Platelet Derived Growth Factor, similarly to that of other serum growth factors or tumor promoters, is mediated through rise in polyamine levels.
D000818:Animals; D002460:Cell Line; D000518:Eflornithine; D004790:Enzyme Induction / Q000187:drug effects; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009952:Ornithine / Q000031:analogs & derivatives / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D065108:Ornithine Decarboxylase Inhibitors; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D065108:Ornithine Decarboxylase Inhibitors; D010982:Platelet-Derived Growth Factor; D009952:Ornithine; D009955:Ornithine Decarboxylase; D013936:Thymidine; D000518:Eflornithine