In vivo cytogenetic studies on mice exposed to ethylene dibromide.
The pesticide, ethylene dibromide (EDB), was evaluated with in vivo cytogenetic assays to determine its genotoxicity. CD1 male mice were exposed to EDB through intraperitoneal injections. Bone marrow cells isolated from femora were analyzed for sister-chromatid exchange (SCE), chromosome aberration and micronucleus formation. The results showed that only certain concentrations of EDB tested caused a slight but significant increase in SCEs and chromosome aberrations. However, these increases were not dose-related. No increase in the polychromatic erythrocytes with micronuclei was observed following EDB exposure. Also, EDB did not cause cell-cycle delay in comparison with controls. Thus, it appears that EDB is not an effective genotoxic agent in vivo in mice.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D015946:Ethylene Dibromide / Q000008:administration & dosage / Q000494:pharmacology*; D006842:Hydrocarbons, Brominated / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D012854:Sister Chromatid Exchange
D006842:Hydrocarbons, Brominated; D015946:Ethylene Dibromide