[Determination of adenine nucleotides by thin-layer chromatography combined with the dansylation reaction].
A new method was developed for the qualitative and quantitative determining of adenine nucleotides, based on thin-layer chromatography and dansilation. Chromatography mixtures 1,4-dioxan-water-ammonia (40:20:8) or 1,4-dioxan-water-propanol 2-ammonia (40:20:10:8) were used for separation of dansilated derivatives of ATP. ADP, AMP. The intensity of luminescence and the areas of spots served as measures of nucleotide quantities and were estimated using a microdensitometer. The method was applied to estimate the adenine nucleotide pool in E. coli K-12 cells.
D000227:Adenine Nucleotides / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods; D003619:Dansyl Compounds / Q000494:pharmacology*; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects; D010783:Photometry / Q000379:methods
D000227:Adenine Nucleotides; D003619:Dansyl Compounds
Opredelenie adeninovykh nukleotidov metodom tonkosloĭnoĭ khromatografii v sochetanii s reaktsieĭ dansilirovaniia.