Isolation and cultivation of Plasmodium falciparum using adult bovine serum.
RPMI 1640 medium supplemented with adult bovine serum and hypoxanthine was superior to human serum-supplemented medium for the isolation of new strains of Plasmodium falciparum in Sudan. Similar observations in Indonesia have since confirmed our results. The chloroquine sensitivity of new isolates was identical in either human or bovine serum. Once acclimated to culture conditions P. falciparum strains grew better when using human serum. Erythrocyte-specific antibody present in adult bovine serum slightly inhibited merozoite invasion of uninfected cells. Removal of this cross-reactive antibody from bovine serum increased parasite multiplication to the level obtained in human serum.
D000818:Animals; D001769:Blood; D002417:Cattle / Q000097:blood; D002738:Chloroquine / Q000494:pharmacology; D003470:Culture Media; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D010963:Plasmodium falciparum / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D013048:Specimen Handling; D013696:Temperature
D003470:Culture Media; D002738:Chloroquine