NK-cell stimulating properties of a membrane proteoglycane from non-capsulated Klebsiella pneumoniae biotype a.
Upon testing the individual fractions of a composite bacterial vaccine for biological activities, a potent immuno-stimulatory capacity could be demonstrated within a crude membrane proteoglycan preparation from Klebsiella pneumoniae. One of its characteristic features was the capacity to induce alpha-type interferon and increased NK activity in vivo in mice, following intraperitoneal or oral administration. A highly purified fraction from the crude preparation was obtained using alkaline hydrolysis, delipidation and size fractionation. This fraction was shown to be a very potent inducer of NK cells in vivo or in vitro, where in the latter systems concentrations as low as 0.1 microgramme per ml were highly efficient.
D000276:Adjuvants, Immunologic / Q000276:immunology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D007274:Injections, Intraperitoneal; D007370:Interferon Type I / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology*; D007711:Klebsiella pneumoniae; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011509:Proteoglycans / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D000276:Adjuvants, Immunologic; D001425:Bacterial Outer Membrane Proteins; D007370:Interferon Type I; D011509:Proteoglycans