[In vitro malondialdehyde formation in brain structure: a method to characterize antihypoxic properties].
In vitro generated free radicals (ascorbic acid-ferric salt-mixture or hydrogen-peroxide) result in lipid peroxidation on brain cellular fractions and striatum slices an decrease of the stimulated dopamine release from rat striatum slices. Besides cysteamine, alpha-tocopherol, pyrogallol and chelating agents also tisochromide shows an antioxidative activity on lipid peroxidation induced by ascorbic acid-ferric salt-mixture. Nootropic drugs like piracetam, methylglucamine orotate, meclofenoxate hydrochloride and nicergoline are ineffective to mitochondrial malondialdehyde generation. On the other hand, piracetam exhibits a limited effect on oxidative damage of striatum slices. For that reason, the antihypoxic activity of these drugs is not accompanied by any antioxidative component and presumes a relatively high degree of tissular organization.
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008314:Malonates / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010889:Piracetam / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism
D008054:Lipid Peroxides; D008314:Malonates; D008315:Malondialdehyde; D004492:Edetic Acid; D002374:Catalase; D001205:Ascorbic Acid; D010100:Oxygen; D004298:Dopamine; D010889:Piracetam
In vitro-Malondialdehyd-Bildung in Hirnstrukturen: Eine Methode zur Charakterisierung antihypoxischer Eigenschaften.