Synthesis and structure of proteoglycan core protein.
Studies of the structure and synthesis of cartilage proteoglycan core protein have been carried out. Deglycosylation of completed, secreted proteoglycan by HF-pyridine treatment yielded an intact homogeneous core protein of approximately 210,000 daltons, with a blocked amino-terminus. Greater than 95% of chondroitin sulfate chains and 80% of N- and O-linked oligosaccharides were removed by the procedure, which made the product an excellent xylosyltransferase acceptor. Little alteration of core protein structure occurred during the HF-pyridine treatment as shown by complete immunoreactivity with antiserums prepared against hyaluronidase-digested proteoglycan. In other studies, the initially synthesized precursor for proteoglycan core protein was found to be approximately 376,000 daltons and localized to the rough membrane fractions. This precursor already contained N-linked oligosaccharides, and was also able to accept xylose, thereby initiating chondroitin sulfate chains. The precursor was translocated intact in an energy-dependent manner to smooth membrane-Golgi fractions where further processing of high mannose type of oligosaccharides and addition of glycosaminoglycan chains occurred. The subcellular distribution pattern of the chondroitin sulfate-synthesizing enzymes corroborated the proposed topological modifications of the proteoglycan core protein precursor.
D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006821:Hyaluronoglucosaminidase; D066298:In Vitro Techniques; D008970:Molecular Weight; D010430:Pentosyltransferases / Q000378:metabolism; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis*; D000097608:UDP Xylose-Protein Xylosyltransferase
D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases; D006821:Hyaluronoglucosaminidase; D010430:Pentosyltransferases; D011498:Protein Precursors; D011509:Proteoglycans; D000097608:UDP Xylose-Protein Xylosyltransferase