Detection of Plasmodium falciparum using a synthetic DNA probe.
A labeled synthetic polynucleotide representing a repetitive sequence from Plasmodium falciparum was hybridized with genomic DNA spotted on nitrocellulose. After an overnight exposure, 0.1 ng of P. falciparum DNA was specifically detected and 0.01 ng was detected after an exposure of 1 week. The synthetic probe showed no cross-hybridization with host DNA or with DNA isolated from other species in the phylum Apicomplexa, P. vivax and Babesia species. Since synthetic DNA is easily prepared, the observed sensitivity and specificity suggests that synthetic DNA probes would be generally useful in diagnosis.
D000818:Animals; D001345:Autoradiography; D001403:Babesia / Q000235:genetics; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008288:Malaria / Q000175:diagnosis; D051379:Mice; D009693:Nucleic Acid Hybridization; D010963:Plasmodium falciparum / Q000235:genetics*; D010966:Plasmodium vivax / Q000235:genetics
D004247:DNA