Computer-controlled in-vitro simulation of multiple dosing regimens.
The bactericidal effect of gentamicin on Pseudomonas aeruginosa ATCC 27853 was investigated in a computer controlled dynamic in-vitro model, which allows the simultaneous simulation of three different dosing regimens for several days. The same total dose reduced cfu-counts of Pseudomonas aeruginosa most effectively, when administered with peak concentrations of 32 mg/l every 32 h, whereas the other dosing regimens with peak concentrations of 16 mg/l every 16 h and 8 mg/l every 8 h were distinctly less effective following the second and subsequent doses. It was shown that the use of a microcomputer facilitates the in-vitro investigation of multiple dosing regimens but counting of cfu cannot be substituted by automatic measurements of turbidity when rapid bactericidal effects occur.
D003201:Computers; D005839:Gentamicins / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
D005839:Gentamicins