Neurospora glucamylase and a mutant affected in its regulation.
Neurospora glucamylase is a glucose-repressible extracellular enzyme. The enzyme was purified to homogeneity and found to have a molecular weight of 82,000 and to release glucose from either maltose or amylose. The rate of glucamylase synthesis increases more than 100-fold when cells are transferred from a glucose-containing medium to a glucose-free medium. Increased from a glucose-containing medium to a glucose-free medium. Increased production of glucamylase begins within 30 min of the transfer. Glucamylase is rapidly secreted into the medium. A mutant affecting the ability of glucose to repress the synthesis of the glucose-repressible extracellular enzymes glucamylase and invertase has been isolated and studied. The mutant constitutively synthesizes and secretes a glucamylase which is indistinguishable from the wild-type enzyme.
D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D005959:Glucosidases / Q000235:genetics*; D009154:Mutation; D009491:Neurospora / Q000201:enzymology / Q000235:genetics*
D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase