Amphotericin B enhances efficiency of DNA-mediated gene transfer in mammalian cells.
Using plasmids containing the genes for thymidine kinase (tk) and neomycin resistance (neo), we have shown that DNA-mediated genotypic transformation of L and Chinese hamster ovary (CHO) cells is increased several-fold by the presence of the sterol-binding polyene antibiotic, amphotericin. Transformation into the same host cells, using genomic DNA, was also enhanced by amphotericin. Phenotypic expression of beta-galactosidase activity of a plasmid containing the gene for the enzyme was also markedly elevated when the antibiotic was added at transfection. Other sterol-binding polyene antibiotics also showed activity in these DNA-mediated gene transfer assays.
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D005260:Female; D007739:L Cells; D051379:Mice; D009355:Neomycin / Q000494:pharmacology; D010053:Ovary; D010957:Plasmids; D011815:R Factors; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection / Q000187:drug effects; D014170:Transformation, Genetic / Q000187:drug effects*; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D000666:Amphotericin B; D004247:DNA; D013937:Thymidine Kinase; D001616:beta-Galactosidase; D009355:Neomycin