Enhanced luminescence enzyme immunoassay for factor VIII related antigen.
A sandwich enzyme immunoassay for plasma factor VIII related antigen has been developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results on a series of 57 specimens agreed with results obtained by immunoelectrophoresis (correlation coefficient = 0.97).
D000941:Antigens / Q000032:analysis*; D001779:Blood Coagulation Factors / Q000032:analysis*; D005169:Factor VIII / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D014841:von Willebrand Factor / Q000032:analysis*
D000941:Antigens; D001779:Blood Coagulation Factors; D014841:von Willebrand Factor; D005169:Factor VIII