Preparative isolation of plasmid DNA with sedimentation field flow fractionation.
Sedimentation field flow fractionation (SFFF) can be used to isolate plasmids preparatively from crude cellular lysates. Total purification time is about 3/4 day, including lysate preparation. The purity and yield of plasmids isolated by SFFF appear to be at least equivalent to those prepared by traditional methods. Molecular-weight data also are supplied rapidly by SFFF without the need for standards.
D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000032:analysis; D007202:Indicators and Reagents; D010957:Plasmids
D004269:DNA, Bacterial; D007202:Indicators and Reagents