Use of purified antipeptide sera of selected specificity for the detection of the simian virus 40 large T antigen by Western blotting.
Western blotting was used as a powerful alternative to immunoprecipitation for the detection of the simian virus 40 (SV40) large tumor (T) antigen. After resolution by electrophoresis on a SDS-polyacrylamide gel of a [35S]methionine labeled crude extract from SV40 infected monkey kidney cells, the separated proteins were transferred electrophoretically on nitrocellulose paper. T antigen was detected on nitrocellulose strips by using for the first time, specific, purified antipeptide monoclonal antibodies directed against the N- and C-terminal portions of the molecule, and 125I-labeled Protein A.
D000818:Animals; D000918:Antibody Specificity; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D007163:Immunosorbent Techniques; D007668:Kidney / Q000032:analysis; D008715:Methionine / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis*
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D014764:Viral Proteins; D008715:Methionine