Production of human interferon-beta in mouse L-cells.
A mouse fibroblast cell-constructed by genetic engineering--expresses glycosylated human interferon-beta constitutively. The cells were cultivated on microcarriers in special bioreactors. Under these conditions they produce a maximum yield of 6 000 units interferon-beta/ml/day at cell densities of about 2,2 X 10(6) cells/ml. The production stage of interferon-beta is longer than one month.
D000818:Animals; D003001:Cloning, Molecular; D003470:Culture Media; D003584:Cytological Techniques; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D007739:L Cells / Q000276:immunology*; D051379:Mice; D008863:Microspheres
D003470:Culture Media; D007370:Interferon Type I