Bovine and human endothelial cell production of neutrophil chemoattractant activity in response to components of the angiotensin system.
Although there is growing evidence that angiotensin II affects macrophage-mediated inflammatory responses, it is unclear whether it can affect neutrophil-mediated inflammatory responses. Because vascular endothelial cells are capable of releasing neutrophil chemoattractant activity, we attempted to determine whether components of the angiotensin system could affect neutrophil-mediated responses indirectly by stimulating endothelial cells to release neutrophil chemoattractant substances. Cultured bovine and human endothelial cells incubated with angiotensin II released neutrophil chemoattractant activity. This activity appeared within 1 minute of exposure to angiotensin II, and was blocked by saralasin, an angiotensin II antagonist. Angiotensin I also caused release of neutrophil chemoattractant activity, but its effect required conversion to angiotensin II. Bradykinin, another substrate for angiotensin-converting enzyme, did not stimulate appearance of chemoattractant. Chemoattractant generation was not inhibited by indomethacin but was blocked by diethylcarbamazine and 5,8,11,14-eicosatetraynoic acid. This study demonstrates that angiotensin II may influence neutrophil accumulation, via production of neutrophil chemoattractant activity by vascular endothelial cells.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000803:Angiotensin I / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000809:Angiotensins / Q000494:pharmacology*; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002216:Captopril / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004049:Diethylcarbamazine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D012504:Saralasin / Q000494:pharmacology
D000809:Angiotensins; D000804:Angiotensin II; D015117:5,8,11,14-Eicosatetraynoic Acid; D000803:Angiotensin I; D002216:Captopril; D012504:Saralasin; D001920:Bradykinin; D004049:Diethylcarbamazine; D007213:Indomethacin