Butyrate formation from glucose by the rumen protozoon Dasytricha ruminantium.
Production of butyrate by the holotrich protozoon Dasytricha ruminantium involves the enzymes of glycolysis, pyruvate:ferredoxin oxidoreductase, acetyl-CoA:acetyl-CoA C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, 3-hydroxyacyl-CoA hydro-lyase, 3-hydroxyacyl-CoA reductase, phosphate butyryltransferase and butyrate kinase. Subcellular fractionation by differential and density-gradient centrifugation on sucrose gradients indicated that all those enzymes except pyruvate:ferredoxin oxidoreductase were non-sedimentable at 6 X 10(6) g-min. Butyrate kinase and phosphate butyryltransferase were associated with the large- and small-granule fractions. Thus, although metabolic reactions necessary for butyrate production proceed predominantly in the cytosol, hydrogenosomes play a key role in the conversion of pyruvate into acetyl-CoA.
D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D020148:Butyric Acid; D002498:Centrifugation; D016798:Ciliophora / Q000201:enzymology / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology
D002087:Butyrates; D020148:Butyric Acid; D005947:Glucose