[3H]Oestradiol metabolism by 18-day rat interstitial cells in culture and the effect of FSH: presence of 16 alpha-hydroxylase.
The metabolism of [3H]oestradiol by interstitial cells in culture prepared from 18-day old rat testes was investigated. Interstitial cells were able to convert [3H]oestradiol to [3H]oestriol as confirmed by recrystallization of oestriol to constant specific activity from samples containing cells and not from controls. This demonstrated for the first time the presence of 16 alpha-hydroxylase in rat testicular interstitial cells. The effect of in vitro FSH treatment on the cells in culture was also investigated. FSH failed to affect 16 alpha-hydroxylase activity since we could not demonstrate a significant difference between treated and untreated preparations. The 16 alpha-hydroxylation of phenolic steroids is widely regarded as the major pathway of oestrogen metabolism in mammals. This metabolic step significantly reduces the biological activity of oestradiol. The presence of 16 alpha-hydroxylase in interstitial cells suggests that it may play a role in inactivating the oestradiol that is produced in the Leydig cells and thus prevent its intracellular accumulation. Such activity may conceivably play a role in the overall local "fine tuning" of androgen biosynthesis.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D002478:Cells, Cultured; D000072467:Cytochrome P450 Family 2; D004958:Estradiol / Q000378:metabolism*; D004964:Estriol / Q000096:biosynthesis; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D039181:Steroid 16-alpha-Hydroxylase; D013250:Steroid Hydroxylases / Q000378:metabolism*
D004958:Estradiol; D005640:Follicle Stimulating Hormone; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C450279:CYP2C11 protein, rat; D000072467:Cytochrome P450 Family 2; D039181:Steroid 16-alpha-Hydroxylase; D004964:Estriol