Biotin-avidin amplified ELISA for quantitation of human IgA.
A biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) for the measurement of human IgA in serum, colostrum, gastric juice, and supernatants of in vitro mitogen-stimulated human peripheral blood mononuclear cells is described. The ELISA reproducibly detects as little as one nanogram of IgA in a fifty microliter sample (20 ng/ml).
D001360:Avidin; D001710:Biotin; D003126:Colostrum / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005750:Gastric Juice / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis*; D007962:Leukocytes / Q000276:immunology; D008895:Milk, Human / Q000276:immunology
D007070:Immunoglobulin A; D001360:Avidin; D001710:Biotin