[DNA damage and repair in the steps for obtaining biopreparations].
The change in the number of single-strand breaks in the genome of E. coli cells was being studied during centrifugation and drying of biomass used as raw material for producing biopreparations. The results obtained allowed the authors: (1) to establish the value of DNA damage at the two stages mentioned above and to reveal that structural integrity of the genome is mostly damaged if the humidity of dried biomass is below 15%; (2) to demonstrate that the repair of bacterial genome damages occurs after centrifugation and superficial dehydration, but it is not observed after deep dehydration.
D001688:Biological Products / Q000302:isolation & purification*; D002498:Centrifugation; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D003890:Desiccation; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial
D001688:Biological Products; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded
Povrezhdenie i reparatsiia DNK na étapakh razrabotki biopreparatov.