CGA-7 and HHF, two monoclonal antibodies that recognize muscle actin and react with adherent cells in human long-term bone marrow cultures.
The CGA-7, a monoclonal antibody that reacts with smooth muscle cell actin but not with endothelial cell or fibroblast actin, and HHF, a monoclonal antibody that reacts with smooth muscle, skeletal muscle, and cardiac muscle actin, both recognize microfilaments present within adherent cells from actively hematopoietic human long-term marrow cultures. Macrophages, monocytes, and cultured marrow fibroblasts do not react with either antibody. These data suggest that the anti-actin antibodies may serve as useful markers for in vitro microenvironmental cells and lend support to the hypothesis that stromal cells from long-term marrow cultures are different from marrow fibroblasts and may constitute a unique cell lineage.
D000199:Actins / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002478:Cells, Cultured; D006801:Humans; D007124:Immunoenzyme Techniques; D013997:Time Factors
D000199:Actins; D000911:Antibodies, Monoclonal