Radioiodination of a photoactivatable heterobifunctional reagent.
The N-hydroxysuccinimide ester of 4-azidosalicylic acid, a photoactivable heterobifunctional reagent, can be radioiodinated. The low efficiency (3%) of the radioiodination by a previously published method (I. Ji and T. H. Ji, 1982, Anal. Biochem. 121, 286-289) has been increased to 63% by substituting the solvent, acetone, with others such as aqueous acetonitrile, dimethylformamide, or dimethyl sulfoxide. The resulting 125I reagent was used for derivatizing human choriogonadotropin. The radioactive hormone derivative was crosslinked to the alpha beta dimer upon photolysis.
D001386:Azides; D006063:Chorionic Gonadotropin; D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D011865:Radioisotope Dilution Technique; D012459:Salicylates
D001386:Azides; D006063:Chorionic Gonadotropin; D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D012459:Salicylates; C046019:4-azidosalicylic acid N-hydroxysuccinimide ester