Comparison of polyclonal antiserum versus monoclonal antibodies for the rapid diagnosis of influenza A virus infections by immunofluorescence in clinical specimens.
A pool of monoclonal antibodies was compared with polyclonal antiserum for the rapid detection of influenza A virus in 28 clinical specimens by immunofluorescence. Monoclonal antibodies showed higher sensitivity (69 versus 46%) and accuracy (86 versus 75%) and easier slide interpretation than did polyclonal antiserum. The procedure proved useful for rapid detection of a community outbreak of influenza A virus infection.
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D007251:Influenza, Human / Q000175:diagnosis*; D009302:Nasopharyngeal Diseases / Q000382:microbiology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral