[Effect of glucose metabolic products on the growth of a recombinant Escherichia coli strain--a superproducer of DNA-polymerase].
The dynamics of glucose metabolites production by Escherichia coli CM 5199 was studied under the conditions of batch and continuous cultivation. Acetate and ethanol were shown to be accumulated in the cultural broth in considerable amounts, and the rate of their synthesis was directly proportional to the specific growth rate of the culture. Acetate inhibited E. coli growth as was found in experiments conducted in a turbidostat regimen. As a result, the specific growth rate of the strain decreased during both batch and continuous cultivation.
D000332:Aerobiosis; D003470:Culture Media / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis*; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D011995:Recombination, Genetic; D013997:Time Factors
D003470:Culture Media; D004259:DNA-Directed DNA Polymerase; D005947:Glucose
Vliianie produktov metabolizma gliukozy na rost rekombinantnogo shtamma Escherichia coli--sverkhprodutsent DNK-polimerazy.