Polyclonal induction of immunoglobulin synthesis by feline leukocytes as identified in a reverse hemolytic plaque assay.
Optimal conditions of culture and assay for identification of feline immunoglobulin-secreting mononuclear cells were determined for the staphylococcal protein A-reverse hemolytic plaque assay (SpA-RHPA). Hemolytic plaques were most distinct and numerous when peripheral blood mononuclear cells were stimulated with 6.9 micrograms/ml pokeweed mitogen for 7 days. Immunoglobulin-secreting cells were identified morphologically within a zone of hemolysis utilizing a 1:5 dilution of rabbit anti-cat IgG and a 1:30 dilution of guinea pig complement as developing reagents. The SpA-RHPA system should contribute to an understanding of normal feline T- and B-lymphocyte interactions and will likely aid in the identification and understanding of immune cell dysfunctions associated with chronic feline leukemia virus infection.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D002415:Cats; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D011043:Pokeweed Mitogens; D012756:Sheep; D013205:Staphylococcal Protein A
D007136:Immunoglobulins; D011043:Pokeweed Mitogens; D013205:Staphylococcal Protein A