Cell surface glycosaminoglycans (GAGs) of cultured chick fibroblasts. Modifications in relation to the stage of embryo development.
We have investigated the changes in glycosaminoglycan (GAG) composition between cultured fibroblasts derived from 8- and 16-day chick embryos. GAG composition has been studied after [3H]glucosamine and [35S]sulfate labeling. Both the 8- and 16-day embryo fibroblasts were found to contain hyaluronic acid (HA), dermatan sulfate (DS), heparan sulfate (HS) and chondroitin sulfates (CS), the latter being the major component in 8- and 16-day cells. These four GAGs were quantified after their separation using cellulose acetate electrophoresis. The amounts of HA and CS were respectively shown to increase 2-fold and 4-fold between the 8th and 16th day of development, whereas the amounts of HS and DS resp. diminished 2.5-fold and 1.2-fold. These results show that the relative proportions of the different GAGs alter during embryo development. The fibroblasts from 8-day-old embryos detached more rapidly from the culture dishes than the cells from 16-day-old embryos when treated with trypsin. However, this difference was not directly related to the different GAG content.
D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D002462:Cell Membrane / Q000032:analysis*; D002478:Cells, Cultured; D002642:Chick Embryo / Q000032:analysis / Q000166:cytology* / Q000254:growth & development; D002809:Chondroitin Sulfates / Q000032:analysis; D003871:Dermatan Sulfate / Q000032:analysis; D004588:Electrophoresis, Cellulose Acetate; D005347:Fibroblasts / Q000032:analysis* / Q000166:cytology; D006025:Glycosaminoglycans / Q000032:analysis*; D006497:Heparan Sulfate / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D014357:Trypsin
D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D014357:Trypsin