Choriogonadotropin measured with the Tandem-E immunoenzymetric assay system.
We evaluated the Hybritech Tandem-E procedure for quantifying choriogonadotropin (hCG) in human serum. In this "sandwich"-type assay, two monoclonal antibodies directed against different regions of the hCG molecule are used, one coated on a plastic bead, the second conjugated to alkaline phosphatase. The assay can detect as little as 1.0 int. unit of the hormone per liter, shows a linear response up to at least 200 int. units/L, and has good precision. By prolonging the incubations for formation of the sandwich and for substrate hydrolysis, one can achieve higher sensitivity at the expense of a narrower linear range. Correlation with a conventional radioimmunoassay for the beta subunit of hCG was generally excellent, but in one instance the Tandem-E gave an apparently false positive result.
D000328:Adult; D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin / Q000097:blood*; D003429:Cross Reactions; D005189:False Positive Reactions; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000175:diagnosis; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D011933:Reagent Kits, Diagnostic; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone