[Metabolism of 1-(2-tetrahydrofuryl)-5-fluorouracil (FT-207) in human maxillary cancer (OKK) cells in culture].
Incubation of FT-207 with cytosol obtained from OKK cells but not from HEPM cells in vitro resulted in marked formation of the active metabolite, 5-FU. Degradation of 5-FU measured by FBA formation was very low in OKK cytosol but high in HEPM cytosol. High activity of 5-FU formation and low activity of 5-FU degradation in tumor cells might be responsible for increased 5-FU levels in the tumors of patients. Inhibition of OKK cell growth by FT-207 occurred in parallel with an appearance of 5-FU in the nucleus. We presume that a factor present in OKK cytosol is a soluble enzyme.
D002455:Cell Division; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D003600:Cytosol / Q000378:metabolism; D005472:Fluorouracil / Q000031:analogs & derivatives* / Q000096:biosynthesis; D006801:Humans; D008441:Maxillary Neoplasms / Q000378:metabolism* / Q000473:pathology; D005641:Tegafur / Q000378:metabolism*
D005641:Tegafur; D005472:Fluorouracil