Fetal rat brain hemisphere tissue in nonadherent stationary organ culture.
A simple organ culture system for brain tissue is described. Fragments of fetal rat brain hemisphere tissue are explanted to multiwell dishes base-coated with semisolid agar. In this system nonadherent organ culture can be performed for at least 50 days. Cell migration, biochemical and morphological differentiation and the formation of a layered architecture seem to mimic some of the phenomena occurring in the developing rat brain in vivo. The fragments may therefore be a useful organ culture model for nervous tissue.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000648:ultrastructure; D002454:Cell Differentiation; D002470:Cell Survival; D005333:Fetus; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009024:Morphogenesis; D009924:Organ Culture Techniques / Q000379:methods*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D051381:Rats; D013997:Time Factors
D005904:Glial Fibrillary Acidic Protein; D010751:Phosphopyruvate Hydratase