[Hybridoma cloning by the end-point dilution method].
Hybridomas producing monoclonal antibodies to tick-borne encephalitis, Venezuelan equine encephalomyelitis, and vaccinia viruses were cloned and recloned by the end-point dilution method in 96-well Linbro plates on a cell feeder layer. With decreasing of the cell seed dose the average number of clones per well decreased and the effectiveness of clone production increased. When an average of one cell per well was introduced, the portion of vells with single clones for hybridomas of various origins ranged from 5.4% to 37.5%. As a rule, the first cloning resulted in a significant rise in titres of monospecific immunoglobulins secreted by hybridomas and improved growth characteristics of the hybrid cell population. Repeated cloning required in most cases for stability of the hybrid line was usually not accompanied by changes in the productivity of the cell cultures.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D003584:Cytological Techniques / Q000295:instrumentation; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D014616:Vaccinia virus / Q000276:immunology
D000911:Antibodies, Monoclonal
Klonirovanie gibridom metodom predel'nykh razvedeniĭ.