Enzyme immunoassay of teichoic acids from Listeria monocytogenes.
Amino groups were introduced into Listeria monocytogenes teichoic acids by reductive amination, and the product was coupled to biotin. Teichoic acids were assayed by their binding to specific antibody adsorbed to a solid phase, followed by detection of the antigen-antibody complex by horseradish peroxidase-avidin. Less than 20 ng of teichoic acid was detectable.
D000907:Antibodies, Bacterial / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis; D001710:Biotin / Q000276:immunology; D007124:Immunoenzyme Techniques; D008089:Listeria monocytogenes / Q000032:analysis* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000276:immunology; D013682:Teichoic Acids / Q000032:analysis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids; D001710:Biotin