Basement membrane and connective tissue proteins in early lesions of Kaposi's sarcoma associated with AIDS.
Nearly one-third of all young homosexual men diagnosed as having acquired immune-deficiency syndrome (AIDS) develop a disseminated form of dermal Kaposi's sarcoma (KS). Although the histogenesis of KS cells is unclear, certain evidence suggests that the aberrant cells are of endothelial derivation. We have examined the presence and distribution of connective tissue-specific and basement membrane-specific macromolecules by indirect immunofluorescence and immunoperoxidase staining of frozen sections in early cutaneous lesions of KS from individuals with AIDS. The KS cells typically line the spaces between collagen bundles of the reticular dermis. When stained for the connective tissue-specific glycoprotein fibronectin, all Kaposi's sarcoma lesions showed an intense staining pattern, revealing a complex array of linear deposits of antigen that outlined the exterior surface of the collagen bundles. Antibodies to laminin and type IV collagen, both basement membrane-specific macromolecules, produced an intense staining pattern similar to that found with the anti-fibronectin antiserum, indicating that all 3 antigens are closely codistributed. In contrast, antibodies to type I collagen, the major collagen of the dermis, uniformly stained the collagen bundles in the KS lesions and in the normal control skin. Antiserum to factor VIII-associated antigen, an antigen specific to blood vascular endothelium, frequently stained the KS lesions but the staining pattern was diffuse and of variable intensity. The results suggest that KS cells are derived from the endothelium of the blood microvasculature and maintain their secretory phenotype of secreting basement membrane-specific macromolecules.
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis; D001485:Basement Membrane; D003094:Collagen / Q000032:analysis*; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D005353:Fibronectins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D012514:Sarcoma, Kaposi / Q000032:analysis / Q000150:complications*; D014841:von Willebrand Factor
D000941:Antigens; D005353:Fibronectins; D007797:Laminin; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D014841:von Willebrand Factor; D005169:Factor VIII; D003094:Collagen