Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles. Interaction with cytoskeletal protein - vinculin.
A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinculin immobilized on polysterene surface. It is suggested that this 175-kDa protein may be involved in physical connection between microfilaments and cell membrane.
D000818:Animals; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005895:Gizzard, Avian / Q000032:analysis; D005779:Immunodiffusion; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009130:Muscle, Smooth / Q000032:analysis*; D009206:Myocardium / Q000032:analysis*; D016596:Vinculin
D008565:Membrane Proteins; D009124:Muscle Proteins; D016596:Vinculin