Sympathetic neurons extend neurites in a culture medium containing cyanide and dinitrophenol but not iodoacetate.
A culture medium circulated through the rat heart and supplemented with insulin, transferrin and nerve growth factor leads to a massive proliferation of neurite outgrowth from neurons of peripheral sympathetic ganglia of the chick embryo. Addition of 1 mM cyanide or 50 microM dinitrophenol to such medium for 2 days had no adverse effect on the neurite outgrowth and ATP content of these neurons. However, 0.5 microM iodoacetate lowered ATP content 65% without affecting the number of surviving neurons up to 2 days. Only when ATP content was reduced to 80% by 2.5 microM iodoacetate was the number of surviving neurons significantly reduced (30%). It is concluded that the glycolytic pathway is the major route of ATP synthesis in embryonic sympathetic neurons maintained in culture, and only a small fraction of ATP is utilized for the survival and neurite extension.
D019297:2,4-Dinitrophenol; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D003470:Culture Media; D003486:Cyanides / Q000494:pharmacology*; D004140:Dinitrophenols / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology*; D019807:Iodoacetic Acid; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D013564:Sympathetic Nervous System / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D003486:Cyanides; D004140:Dinitrophenols; D007328:Insulin; D007461:Iodoacetates; D009414:Nerve Growth Factors; D014168:Transferrin; D000255:Adenosine Triphosphate; D019297:2,4-Dinitrophenol; D019807:Iodoacetic Acid