[New method of encapsulating isolated nuclei into a lipid membrane].
A new method of the coating of isolated cell nuclei with additional phosphatidylcholine membranes is described. The additional membrane was visualized under the electron microscope using ferritin as a label. The coating diminished leakage of the content of the nuclei and restricted permeability of the nuclei for exterior macromolecules.
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D003584:Cytological Techniques; D005347:Fibroblasts / Q000648:ultrastructure; D008081:Liposomes / Q000494:pharmacology; D008099:Liver / Q000648:ultrastructure; D008563:Membrane Lipids / Q000494:pharmacology*; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D008907:Mink; D010713:Phosphatidylcholines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008081:Liposomes; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010713:Phosphatidylcholines
Novyĭ metod zakliucheniia izolirovannykh iader v lipidnuiu obolochku.