[Isolation and characteristics of the thermolabile enterotoxin from Escherichia coli strain H74-114].
The modified method for isolation and purification of LT-enterotoxin from Escherichia coli strain H74-114 has been proposed. Modification is based on exclusion of concentration of bacterial cell lysates by 3-5 fold dilution and changing PH during chromatography. The proposed modification permitted to increase the yield of purified and active product 8-10 fold. The obtained preparations are homogenic due to PAAG-SDS data and immunochemical analysis. B-component of TL-enterotoxin is shown to consist of 4 subunits B in contrast to B-component of choleragen, consisting of 5 subunits. The mol mass of A component of both toxins is identical but in contrast to choleragen the component of LT-enterotoxin is not subject to proteolysis.
D001427:Bacterial Toxins / Q000032:analysis / Q000302:isolation & purification*; D002772:Cholera Toxin / Q000032:analysis; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000032:analysis / Q000302:isolation & purification*; D004926:Escherichia coli / Q000032:analysis* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D007120:Immunochemistry; D008970:Molecular Weight
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli
Poluchenie i kharakteristika termolabil'nogo énterotoksina iz shtamma H74-114 Escherichia coli.