Binding of different antigen-enzyme and antibody-enzyme conjugates by intracellular antibodies in cytoplasm and Golgi complex of plasma cells. A double immunocytochemical study.
Intracellular immunoglobulins in plasma cells were characterized by antigen-enzyme conjugates and anti-immunoglobulin antibody-enzyme conjugates applied in a double immunocytochemical approach. After their assemblage, immunoglobulins in the cytoplasm of anti-TNP anti-body producing plasma cells can be demonstrated both by TNP-enzyme conjugates and by anti-immunoglobulin (mu or gamma chain specific) antibody-enzyme conjugates. Once arrived in the Golgi complex (GC) detection with TNP-enzyme conjugates remains possible, but anti-immunoglobulin anti-body-enzyme conjugates did not bind to a detectable degree. Similar results were obtained in experiments where immunoglobulin-enzyme conjugates were used both as an antigen-enzyme conjugate and as an antibody-enzyme conjugate.
D000469:Alkaline Phosphatase; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000941:Antigens / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D006056:Golgi Apparatus / Q000276:immunology; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000378:metabolism*; D008297:Male; D010950:Plasma Cells / Q000276:immunology*; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007136:Immunoglobulins; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase