Two techniques for detection of antibodies against Corynebacterium (Rhodococcus) equi in horse sera.
Two techniques were developed to detect antibodies against the exosubstance of C. equi called equi-factor. In the first technique serum samples are tested against native equi-factor produced by the growth of C. equi on agar medium. A positive result is manifested by the development of precipitation lines. The second test is based on neutralization of prepurified equi-factor by antibody, resulting in the inhibition of its hemolytic synergism with staphylococcal beta toxin. Sera (125 samples) from horses of different ages, kept in localities with a history of C. equi infections, were examined. The first technique detected 65.6%, and the second 40% of positive cases.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001427:Bacterial Toxins / Q000276:immunology; D003352:Corynebacterium / Q000276:immunology*; D003354:Corynebacterium Infections / Q000276:immunology / Q000662:veterinary*; D006734:Horse Diseases / Q000276:immunology*; D006736:Horses; D005779:Immunodiffusion
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins