Differential expression of H-2Dd and H-2Ld histocompatibility antigens.
To determine why the H-2Dd antigen is expressed on the surface of transfected cells at a rate several-fold higher than an analogously transfected H-2Ld molecule, we analyzed both previously described and new H-2 hybrid genes. These genes were constructed by exchanging domains between H-2 genes. Quantitative radioimmunoassay indicates that the region of the H-2Dd molecule responsible for its enhanced expression resides in the polymorphic N domain, the first domain of the mature class I molecule.
D000818:Animals; D003001:Cloning, Molecular; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000032:analysis / Q000235:genetics*; D064532:Histocompatibility Antigen H-2D; D007739:L Cells / Q000276:immunology; D008668:Metallothionein / Q000235:genetics; D051379:Mice; D009876:Operon; D012333:RNA, Messenger / Q000032:analysis
D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D012333:RNA, Messenger; D008668:Metallothionein