[Distribution of nervous system-specific enolase (NSE) and S-100 protein in the gastrointestinal tract of congenital aganglionosis rat].
Immunobiochemical and immunohistochemical studies were performed to find out changes in neural and glial components of the intestine in congenital aganglionosis rats. Nervous system-specific enolase (NSE) was used as a marker of the neural component. S-100 protein was used as a marker of the glial component. NSE and S-100 protein were quantitated by highly sensitive immunoassay. And distribution of NSE and S-100 protein of the intestine was studied by immunohistochemical methods in fetal rats of various gestational age. The results of our studies were followed; Significant decrease in NSE and S-100 protein was found in the aganglionic segment. Although there was no decrease in NSE, significant decrease in S-100 protein was found in the ganglionic segment just proximal to the aganglionic narrow segment. In the studies using rat embryos, NSE positive cells and S-100 protein positive cells were identified in the more caudal intestine with increasing gestational age. In addition, NSE positive cells appeared in the intestine few days earlier than S-100 protein positive cells did. In conclusion, our studies disclosed that there was abnormality of the glial component as well as the neural component in the intestine of Hirschsprung's disease.
D000818:Animals; D000831:Animals, Newborn; D004064:Digestive System / Q000032:analysis* / Q000201:enzymology; D005333:Fetus / Q000378:metabolism; D005865:Gestational Age; D006627:Hirschsprung Disease / Q000201:enzymology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D010751:Phosphopyruvate Hydratase / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000032:analysis*
D009418:S100 Proteins; D010751:Phosphopyruvate Hydratase