Increased ovulation rate of adult ewes treated with anti-bovine LH antiserum during the normal breeding season.
Adult Suffolk ewes (n = 14) were treated on d 10 of the estrous cycle with anti-bovine luteinizing hormone (LH) antiserum. Control ewes (n = 10) were treated with normal horse serum. Estrous behavior and the number of corpora lutea and ovarian follicles were examined at the subsequent estrous cycle. Daily plasma concentrations of progesterone (P4), follicle stimulating hormone (FSH) and estradiol were determined before and after treatment. Ewes treated with antiserum had a higher (P less than .05) ovulation rate (2.7 +/- .2) than did controls (2.1 +/- .1). No differences were found in the numbers of large (greater than 5 mm) or small (less than 5 mm) follicles between treatment groups. Estrus was delayed (P less than .025) approximately .6 d/in ewes treated with antisera. Immunoreactive FSH increased (P less than .05) within 1 d after treatment and remained higher than the controls for 5 d. Peak estradiol concentrations occurred on d 17 for treated ewes compared with peak concentrations on d 15 or 16 for control ewes. The P4 concentrations were generally less (P less than .025) in treated ewes throughout the luteal phase of the treatment cycle. These data demonstrate that ovulation rate is increased in ewes treated with LH antiserum. The marked increase in plasma FSH suggests a possible mechanism whereby ovulation rate is enhanced.
D000818:Animals; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000187:drug effects; D004973:Estrus Synchronization; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007106:Immune Sera / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction / Q000662:veterinary; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012756:Sheep / Q000502:physiology*
D007106:Immune Sera; D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone