An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.
A sensitive and quantitative assay for DNA ligase has been developed which is suitable for the analysis of crude cell extracts of yeast. The assay is sufficiently sensitive to detect the low levels of DNA ligase activity remaining in cdc9 mutants of Saccharomyces cerevisiae. Indeed, we have been able to show that this residual activity is temperature-sensitive, thus establishing finally that CDC9 is the structural gene for DNA ligase.
D001483:Base Sequence; D011088:DNA Ligases / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D011118:Polynucleotide Ligases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013696:Temperature; D013698:Templates, Genetic
D011088:DNA Ligases; D011118:Polynucleotide Ligases