[An expedient semi-automatic procedure for the preparation of large quantities of bioindicators especially for use in gas sterilization processes].
Bioindicators serve to test the efficacy of disinfection and sterilization procedures. Such indicators mostly consist of a support (filter paper, as a rule) to which micro-organisms have been fixed by drying. The authors have used a thread as support and a special apparatus for semi-automatic preparation of the bioindicators. The components of the device are either commercially available or may be prepared from commercially available material without difficulty. The principle of the method is as follows: The thread serving as the support is drawn slowly, at constant speed, through the suspension of test organisms and dried in an air stream immediately afterwards. The apparatus consists of a cylindrical glass tube of a few centimeters in diameter, an electric motor slowly rotating the cylinder, a fan, a magnetic stirrer, and an ice-water bath. A small vial containing the germ suspension is immersed in the ice-water bath. The vial is sealed by a screw cap with two glass tubes of about 3 mm inner diameter passing through it. One of the glass tubes being bent in its upper part reaches far down into the vial to leave just enough play for free rotation of a magnetic stirring rod. This tube serves to introduce the thread into the germ suspension. The second straight tube does not reach as far down as the first one. Its lower opening should not be immersed in the germ suspension. This tube serves as a guide for the returning thread. Preparation begins by winding the thread to be soaked with the suspension around the cylinder.(ABSTRACT TRUNCATED AT 250 WORDS)
D001419:Bacteria / Q000254:growth & development*; D005658:Fungi / Q000254:growth & development*; D005740:Gases; D008828:Microbiological Techniques / Q000295:instrumentation; D013242:Sterilization / Q000379:methods*
D005740:Gases
Ein rationelles halbautomatisches Verfahren zur Herstellung grosser Mengen Bioindikatoren, insbesondere für Gas-Sterilisationsverfahren.