Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells.
We have placed human insulin receptor cDNA into a vector under the control of the simian virus 40 (SV40) early promoter and tested its function by transient expression in microinjected Xenopus oocytes and by expression in stably transformed CHO cells. The precursor and the alpha and beta subunits of the receptor were detected by immunoprecipitation from extracts of these cells. The human insulin receptor expressed in CHO cells specifically binds 125I-labeled insulin but not insulin-like growth factor I, displays insulin-stimulated autophosphorylation of the beta subunit, and mediates insulin-stimulated 2-deoxyglucose uptake. We conclude that the human insulin receptor is synthesized, processed normally, and functional in this heterologous cell system.
D000818:Animals; D000911:Antibodies, Monoclonal; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D008970:Molecular Weight; D009865:Oocytes; D010957:Plasmids; D011972:Receptor, Insulin / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D007328:Insulin; D004247:DNA; D011972:Receptor, Insulin; D005947:Glucose