Aging effects on the elastin composition in the extracellular matrix of cultured rat aortic smooth muscle cells.
Elastin accumulation in the extracellular matrix of cultured rat aortic smooth muscle cells was monitored as a function of age. The effect of the animal donor age and time in culture in single or consecutive passages on the cells' ability to accumulate total protein as well as elastin was evaluated. Smooth muscle cells were obtained from animals ranging in age from 2 d to 36 mo. Protein accumulation by the cells based on DNA content was similar regardless of which of the above aging parameters was examined. Although there were significant amounts of elastin present in the extracellular matrix of those cells originating from the younger animals (2 d and 6 wk old), little or none was detected in cell cultures derived from the oldest animals. A soluble elastin-like fraction which was isolated from the cultures of the 2-d-old rats seemed to be lacking in the cultures of cells from the 36-mo-old animals. This observation may, in part, explain the absence of insoluble elastin in the matrix of some cultures obtained from older animals. The data strongly suggest that the age of the donor animal from which the cells originate has the greatest influence on in vitro elastin accumulation.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D004549:Elastin / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000032:analysis*; D007163:Immunosorbent Techniques; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000032:analysis / Q000648:ultrastructure*; D011817:Rabbits; D051381:Rats; D013997:Time Factors
D000596:Amino Acids; D004549:Elastin