Production of macrophage activating factor by human leukemic T cell lines.
Human leukemic T cell lines were tested for their ability to produce a macrophage activating factor. When mouse peritoneal macrophages were cultured for 48 hr in the presence of culture supernatants from cell lines HPB-ALL, CCRF-CEM, or MOLT-4, glucose oxidation via the hexose monophosphate pathway was enhanced by five to seven fold. Culture supernatants from cell line HPB-MLT stimulated the oxidation to a lesser extent. However, cell line CCRF-HSB-2 was essentially inactive as a producer. The active supernatants also stimulated the release of hydrogen peroxide from macrophages, whereas the inactive one did not. Since treatment of the cell lines with 12-o-tetradecanoyl phorbol acetate or phytohemagglutinin had little effect on the production of the factor except HPB-ALL, the cell lines seemed to secrete the factor constitutively. The stimulatory effect was dose-dependent and evident at a concentration as low as a 1/80 dilution. The factor was resistant to heat treatment at 100 C for 20 min, nondialysable and sensitive to protease digestion. The activating factor could be partially purified by anion exchange and gel filtration chromatographies.
D000818:Animals; D002460:Cell Line; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis* / Q000302:isolation & purification; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D008222:Lymphokines; D016215:Macrophage-Activating Factors; D006861:Hydrogen Peroxide; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate