Monitoring of urinary mutagenicity in workers exposed to low doses of 2,4,7-trinitro-9-fluorenone.
A monitoring of the urinary mutagenicity in workers occupationally exposed to low doses of 2,4,7-trinitro-9-fluorenone (TNF) was undertaken. Urine concentrate of 22 exposed workers (11 smokers and 11 nonsmokers) and 18 presumedly unexposed workers (7 smokers and 11 nonsmokers) were assayed for mutagenicity in Salmonella typhimurium strain TA98 with the plate incorporation technique. In this test system none of the urine concentrate was effective as a mutagen, either in the absence or presence of S9. Fifteen urine samples (8 from exposed workers, 7 from referents) were also tested in the microtiter fluctuation assay. With this technique smoking habits were significantly related to urinary mutagenicity in tests performed with metabolic activation. In neither case however was the association between presumed exposure and urinary mutagenicity significant. These results were evaluated on the basis of urinary mutagenicity data obtained from rats exposed to TNF by different routes. It was shown that the observed urinary mutagenicity accounts for a minor fraction of the administered TNF dose (about 0.1 to 0.2%, depending on the route of exposure); thus it is possible that low-level exposure to TNF could escape detection by urinary mutagenicity monitoring.
D000395:Air Pollutants, Occupational / Q000633:toxicity*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005449:Fluorenes / Q000633:toxicity* / Q000652:urine; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens; D011327:Printing; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects
D000395:Air Pollutants, Occupational; D005449:Fluorenes; D009153:Mutagens; C005644:2,4,7-trinitrofluorenone