Mechanisms of leu-enkephalin hydrolysis in human plasma.
The present work describes the kinetics of enkephalin hydrolysis by plasma enzymes and the fragmentation pattern of both the parent peptide and of the first hydrolysis by-products. The degradation kinetics were followed by positive identification of the hydrolysis fragments by chromatographic methods, by amino acid analysis and by scintillation counting of tritium-labeled enkephalin. In addition, the results presented confirm the role of the low molecular weight plasma components in the control of the hydrolysis of the peripherally-released enkephalins.
D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D004743:Enkephalin, Leucine / Q000097:blood*; D006207:Half-Life; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010447:Peptide Hydrolases / Q000097:blood
D000596:Amino Acids; D004743:Enkephalin, Leucine; D010447:Peptide Hydrolases