Intracellular transport of high molecular weight intermediates of acid alpha-glucosidase in human fibroblasts.
Two transient, high molecular weight precursors of human acid alpha-glucosidase were detected by immune precipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The high molecular weight precursors were rapidly converted into lower molecular weight forms corresponding to previously identified intermediates of acid alpha-glucosidase. An accumulation of these precursors was observed in fibroblasts treated with monensin and nigericin, suggesting that these precursors are intermediates of acid alpha-glucosidase undergoing transport through the Golgi complex.
D000818:Animals; D001693:Biological Transport, Active; D001826:Body Fluids / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000201:enzymology*; D005959:Glucosidases / Q000378:metabolism*; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism*; D008970:Molecular Weight; D008985:Monensin / Q000494:pharmacology; D009550:Nigericin / Q000494:pharmacology; D011817:Rabbits; D000520:alpha-Glucosidases / Q000378:metabolism*
D008985:Monensin; D005959:Glucosidases; D000520:alpha-Glucosidases; D009550:Nigericin