beta-Hydroxydecanoylthioester dehydrase: a rapid, convenient, and accurate product distribution assay.
High-performance liquid chromatography on silica gel has been used to separate the products from incubation of substrates with beta-hydroxydecanoylthioester dehydrase (Escherichia coli). Peaks are detected by their absorbances at 230 nm. Following correction for differences in extinction coefficients, comparison of the peak areas reveals the relative amounts of beta-hydroxydecanoate, E-2-decenoate, and Z-3-decenoate thioesters of N-acetylcysteamine.
D002851:Chromatography, High Pressure Liquid; D003543:Cysteamine / Q000031:analogs & derivatives / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology; D054889:Fatty Acid Synthase, Type II; D006836:Hydro-Lyases / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
C047124:3-decenoic acid N-ethylcysteamine thioester; C047122:3-hydroxydecanoic acid N-acetylcysteamine thioester; C047123:2-decenoic acid N-acetylcysteamine thioester; D003543:Cysteamine; D006836:Hydro-Lyases; C047118:3-hydroxyacyl-(acyl-carrier-protein) dehydratase; D054889:Fatty Acid Synthase, Type II