Stimulation and killing of bovine mononuclear leukocytes by bacterial lipopolysaccharide (endotoxin).
Bovine adherent mononuclear leukocytes were incubated with bacterial lipopolysaccharides (LPS) in vitro, and these cells produced a factor that increased the blastogenic reaction of mouse thymocytes to concanavalin A. This factor most resembles interleukin 1. The LPS were also cytotoxic for bovine adherent mononuclear leukocytes in a dose-and time-dependent manner. Cytotoxicosis was determined by the release of cytoplasmic lactic dehydrogenase. This cytotoxicosis was blocked by treating the cells with corticosteroids. Variation in the reaction to LPS occurred in cells collected from the same cow on different days and from cells collected from different cows.
D000818:Animals; D002417:Cattle / Q000097:blood*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004926:Escherichia coli; D005260:Female; D006854:Hydrocortisone / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000201:enzymology / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D011239:Prednisolone / Q000031:analogs & derivatives / Q000494:pharmacology; D012475:Salmonella; D013601:T-Lymphocytes / Q000276:immunology
D008070:Lipopolysaccharides; D008222:Lymphokines; C027779:blastogenic factor; D011239:Prednisolone; D007770:L-Lactate Dehydrogenase; C021322:prednisolone hemisuccinate; C007133:hydrocortisone hemisuccinate; D006854:Hydrocortisone