[An immunochemical assay of pregnanediol 3-glucuronide in urine and its variation in the peri-ovulatory period].
A rapid, simple and sensitive immunochemical assay of pregnanediol 3-glucuronide (Pd-3G) in urine was established. The standard error range of the recovery test was within 0.8 microgram/ml, and the coefficient variation of the within run and day to day precision tests were within 4%. Cross reactivity of various steroids exhibited less than 4%. A significant rise of Pd-3G (mg)/creatinine (g) coincided with the LH surge and continued during the luteal phase in the ovulatory menstrual cycle. In an anovulatory cycle, Pd-3G was found to be under 4 mg/g. This Pd-3G direct assay method could be widely used for assessing ovulation and luteal function.
D000328:Adult; D000858:Anovulation / Q000652:urine; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D008183:Luteal Phase; D008597:Menstrual Cycle; D010060:Ovulation; D011276:Pregnanediol / Q000031:analogs & derivatives* / Q000652:urine
C022270:pregnanediol-3 alpha-glucuronide; D011276:Pregnanediol