Simultaneous measurement of toxicity and mutagenic activity.
The assay of mutagenic activity of toxic drugs is difficult to perform and analyze, because one needs to know the kinetics of both effects in order to draw reliable conclusions. This is the case with niflumic acid (NA), which reduced the viability of S. typhimurium TA1535 100 times in the Ames test, but the background microcolonies show no difference from controls and the number of revertants was not altered by the drug. A test which measures the kinetics of growth of viable bacteria and mutants in liquid medium has been developed and applied to NA. No mutagenic activity was detected and elimination of the toxicity from the medium is suggested.
D002455:Cell Division / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation; D009539:Nicotinic Acids / Q000494:pharmacology*; D009544:Niflumic Acid / Q000494:pharmacology* / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
D009539:Nicotinic Acids; D009544:Niflumic Acid