A Chinese hamster ovary cell mutant resistant to aphidicolin.
A triple (aphr ara-Ar and araCr) mutant (AP7) of Chinese hamster ovary cells resistant to DNA polymerase inhibitors is described. The aphidicolin-resistance of the mutant was stable and inherited as a dominant genetic trait. The DNA polymerase alpha from the wild type (aphs) and the mutant (aphr) cells differed in their elution profiles on DEAE-cellulose chromatography and in their molecular weights which were 192,000 for the wild type (CHO-K-1, AC6a) and 165,000 for the mutant (AP7) enzymes.
D000818:Animals; D016590:Aphidicolin; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D004224:Diterpenes / Q000494:pharmacology*; D004351:Drug Resistance; D005260:Female; D009154:Mutation; D019384:Nucleic Acid Synthesis Inhibitors; D010053:Ovary
D004224:Diterpenes; D019384:Nucleic Acid Synthesis Inhibitors; D016590:Aphidicolin; D004259:DNA-Directed DNA Polymerase