A microtiter modification of granulocyte immunofluorescence.
The granulocyte immunofluorescence test (GIFT) is valuable for detecting allogeneic and autologous granulocyte antibodies. However, the original tube technique (macro GIFT) requires 50-100 microliters of serum and 10(6) granulocytes with time-consuming washing steps which cause cell loss. We report a modification (micro GIFT) using microtiter trays which requires only 20 microliters of serum and 2 X 10(5) granulocytes. The modified method conserves reagents, reduces the time required for washing by 67%, decreases cell loss in washing by 74%, reduces the volume of conjugate required by 67%, and compares favorably with the macro GIFT in accuracy, specificity, and sensitivity.
D001323:Autoantibodies / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000032:analysis; D011786:Quality Control
D001323:Autoantibodies; D007518:Isoantibodies