Expression of surface antigen gene of human hepatitis B virus serotype adr in Escherichia coli.
The construction of an expression plasmid of hepatitis B virus surface antigen (HBsAg) gene from the cloned hepatitis B virus (HBV) genome subtype adr is reported. The expression products of this plasmid in E. coli were detected by means of radioimmunoassay in competitive suppression and polyacrylamide-SDS gel electrophoresis. The presence of a fusion protein containing HBsAg was confirmed.
D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005814:Genes, Viral; D005818:Genetic Engineering; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006801:Humans; D010957:Plasmids
D006514:Hepatitis B Surface Antigens