[Development of an immunoenzyme test system for the quantitative demonstration of influenza virus hemagglutinin in biological specimens].
A test system for the detection and quantitation of influenza virus hemagglutinin (HA) in biological specimens by solid-phase enzyme immunoassay (SPEIA) is described. The test system implies sequential adsorption on polystyrene base of anti-HA guinea pig gammaglobulins, detectable HA-containing antigen, monospecific anti-HA rabbit serum, and peroxidase antirabbit conjugate. Adsorption of the HA-containing antigen is run in the presence of a high concentration of nonionic detergent. The developed method is highly sensitive (0.3-0.5 ng in a specimen) and permits the detection and quantitation of HA in whole influenza virions, preparations of surface glycoproteins and in preparations of hemagglutinin recovered from virus particles. The analysis (after pre-sensitization of the polystyrene adsorbent) takes from 2 to 21/2 hours. The possibility of using the developed test system for control and standardization of inactivated influenza vaccines (whole-virion, split, and subunit) is discussed.
D000818:Animals; D006168:Guinea Pigs; D006389:Hemagglutinins, Viral / Q000032:analysis* / Q000302:isolation & purification; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology*; D011817:Rabbits; D014771:Virion / Q000276:immunology
D006389:Hemagglutinins, Viral; D007106:Immune Sera
Razrabotka immunofermentnoĭ test-sistemy dlia kolichestvennoĭ indikatsii gemaggliutinina virusa grippa v biologicheskikh obraztsakh.