Alloimmunisation to HLA antigens following transfusion with leucocyte-poor and purified platelet suspensions.
24 previously non-transfused patients were given three transfusions of 200 X 10(8) platelets at 14-day intervals. Group I (12 patients) received leucocyte-poor platelet suspensions with a mean contamination of 15 X 10(6) leucocytes per transfusion. Group II (12 patients) received platelets with less than 5 X 10(6) leucocytes per transfusion. 5 patients in group I and no patients in group II developed lymphocytotoxic antibodies (p = 0.037). Platelets with fewer than 5 X 10(6) leucocytes did not seem to stimulate a response to major histocompatibility antigens, but with a small contaminating dose of leucocytes appeared to be highly immunogenic.
D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000166:cytology; D001803:Blood Transfusion; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007518:Isoantibodies / Q000032:analysis*; D016030:Kidney Transplantation; D017708:Leukocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D017713:Platelet Transfusion
D006680:HLA Antigens; D007518:Isoantibodies