Determination of antibodies in dried blood from earlobes of leprosy patients by enzyme-linked immunosorbent assay--a preliminary report.
An enzyme-linked immunosorbent assay (ELISA) was used with soluble antigens of Mycobacterium leprae. All blood samples collected from the earlobes of 109 leprosy patients and 100 healthy controls (from a non-endemic area of leprosy) were absorbed with M. vaccae, BCG, cardiolipin, and lecithin according to the technology of the FLA-ABS test before being tested in the ELISA. The results (at a 1:200 blood dilution) showed that antibody activity gradually increased from TT to LL (mean OD values: TT = 0.43, BT = 0.62, BB = 0.72, BL = 0.84, LL = 0.89), and the rates of positive reactions were 100% in all classifications of patients except TT (66.6%). Antibody activity in the controls was less pronounced than in leprosy patients, their mean OD value being only 0.15. We suggest that the ELISA is highly sensitive and specific for the determination of anti-M. leprae antibodies, and is useful for clinical serodiagnosis and for the study of subclinical infections in leprosy.
D000907:Antibodies, Bacterial / Q000032:analysis*; D001800:Blood Specimen Collection; D004431:Ear, External; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007918:Leprosy / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*
D000907:Antibodies, Bacterial