Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans.
A mutant of Aspergillus niger defective in ornithine transcarbamylase function was transformed with plasmids carrying a functional copy of the argB gene of Aspergillus nidulans after treatment of spheroplasts in the presence of polyethylene glycol and calcium ions. The plasmid pDG3 gave stable transformants at a frequency of 4 per microgram of input DNA. Southern blot analysis of DNA from transformants showed that pDG3 DNA had integrated into the A. niger chromosomes at a variety of locations. The transformants were phenotypically stable for many mitotic divisions. This procedure may potentially be used to insert any gene into the genome of A. niger. A cosmid shuttle vector, pDG1, for cloning in Aspergillus was also constructed.
D001120:Arginine / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics*; D001234:Aspergillus niger / Q000235:genetics*; D004261:DNA Replication; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D009954:Ornithine Carbamoyltransferase / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D001120:Arginine; D009954:Ornithine Carbamoyltransferase