Application of fluorescence polarization to the determination of urinary lysozyme activity.
A fluorescence polarization (FP) technique was applied to the assay of lysozyme activity in human urine. In this assay system, reaction between enzyme and fluorescein-labelled substrate produced a decrease in the fluorescein polarization (P) value. Enzyme activity and rate of decrease of P value exhibited a hyperbolic proportionality between 0.01 mg/L and 10.0 mg/L of lysozyme concentration. The method was compared with a turbidimetric method and the results were comparable with each other. Urinary lysozyme activity in patients with various renal diseases whose renal function had deteriorated was 1.04 +/- 2.55 mg/L (mean +/- S.D.), whereas that of normal controls was 0.13 +/- 0.14 mg/L.
D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005454:Fluorescence Polarization / Q000379:methods*; D006801:Humans; D007477:Ions; D007674:Kidney Diseases / Q000201:enzymology; D009113:Muramidase / Q000652:urine*; D009391:Nephelometry and Turbidimetry; D010457:Peptidoglycan; D013861:Thiocyanates
D005452:Fluoresceins; D007477:Ions; D010457:Peptidoglycan; D013861:Thiocyanates; D009113:Muramidase; D016650:Fluorescein-5-isothiocyanate