Enzyme assays for the identification of gastric fluid.
Simple, reliable procedures for the assay of pepsin and rennin-like enzyme activities are described as a means of identifying gastric fluid-containing samples in forensic science laboratories. These samples are usually vomitus, or stomach contents originating from wounds that perforate the stomach. They may be encountered at scenes or on articles submitted for examination, in fresh form or as dried stains. The pepsin activity assay is based on proteolytic activity with bovine albumin as substrate and the rennin-like activity assay is based on the coagulation of milk protein.
D001826:Body Fluids / Q000032:analysis*; D012085:Chymosin / Q000032:analysis*; D004796:Clinical Enzyme Tests; D005554:Forensic Medicine; D005750:Gastric Juice / Q000201:enzymology*; D006801:Humans; D010434:Pepsin A / Q000032:analysis*
D010434:Pepsin A; D012085:Chymosin