Electron transfer to nitrogenase in Klebsiella pneumoniae. nifF gene cloned and the gene product, a flavodoxin, purified.
The nifF gene of Klebsiella pneumoniae was cloned into a multicopy plasmid in order to construct a strain that synthesizes and retains an elevated concentration of the gene product relative to the wild-type strain. Characterization of the isolated flavodoxin, which serves as an electron donor to nitrogenase, shows unambiguously that it is the product of the nifF gene.
D000596:Amino Acids / Q000032:analysis; D003001:Cloning, Molecular; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005418:Flavodoxin / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005420:Flavoproteins / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D009591:Nitrogenase / Q000378:metabolism*; D010957:Plasmids; D014176:Protein Biosynthesis
D000596:Amino Acids; D005418:Flavodoxin; D005420:Flavoproteins; D009591:Nitrogenase