A simple immunoblotting method after separation of proteins in agarose gel.
A simple and sensitive method for immunoblotting of proteins after separation in agarose gels is described. It involves transfer of proteins onto nitrocellulose paper simply by diffusion through pressure, a transfer which only takes about 10 min. By this method we have demonstrated the existence of multiple molecular forms of the complement factors C3 and factor B in serum from 2 species, man and chicken, after electrophoretic separation in agarose. We have also demonstrated the usefulness of the method for determining the isoelectric point of proteins after isoelectric focusing in agarose.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001797:Blood Protein Electrophoresis / Q000379:methods*; D002645:Chickens / Q000097:blood; D003101:Collodion; D003176:Complement C3 / Q000032:analysis; D011415:Complement Factor B / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing
D000911:Antibodies, Monoclonal; D003176:Complement C3; D003101:Collodion; D011415:Complement Factor B