A 50 kilodalton exoantigen specific to the merozoite release-reinvasion stage of Plasmodium falciparum.
The immunoglobulins G of a human plasma inhibiting in vitro Plasmodium falciparum merozoite reinvasion have been purified and used to immunoprecipitate the antigens released into the culture medium by an [35S]methionine-labeled synchronous culture. Several of the major exoantigens identified were found throughout the entire life cycle; they were also immunoprecipitated from the labeled parasitized cells. Some antigens were found only after the reinvasion stage, and especially a major one of molecular mass 50 kDa and pI 5.5. The latter was not found in the parasitized cells but derived most likely from the processing of a major 126 kDa antigen which disappeared from the parasites during the reinvasion period and which was immunoprecipitated by an anti-50 kDa monoclonal antibody.
D000936:Antigen-Antibody Complex; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008288:Malaria / Q000276:immunology*; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010961:Plasmodium / Q000472:pathogenicity; D010963:Plasmodium falciparum / Q000254:growth & development*; D011506:Proteins / Q000302:isolation & purification*; D013462:Sulfur Radioisotopes
D000936:Antigen-Antibody Complex; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D011506:Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine