Factor VIII related antigen-concanavalin A binding in plasma and serum.
The Factor VIII Related Antigen (FVIIIRAG) was determined by electroimmunodiffusion and the per cent concanavalin A (% con A) binding of FVIIIRAG was determined by crossed affinity electrophoresis (CAIEP) in the plasma and serum of 8 hospitalized patients. The FVIIIRAG in plasma and serum was 669 and 501% of lyophilized plasma, respectively (correlation coefficient, r = 0.92). The FVIIIRAG con A-binding of plasma and serum were 44 and 16%, respectively (p less than 0.01), while the FVIIIRAG-con A binding of lyophilized plasma is 78%. We conclude that plasma has a higher content of electrophoretically slower, high con A affinity FVIIIRAG multimers than serum. The high FVIIIRAG levels of both serum and plasma and the comparatively low plasma FVIIIRAG-con A binding here may reflect the acute phase reaction.
D000328:Adult; D000368:Aged; D000941:Antigens / Q000032:analysis*; D003208:Concanavalin A / Q000378:metabolism*; D004586:Electrophoresis; D005169:Factor VIII / Q000032:analysis / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D010949:Plasma / Q000032:analysis; D014841:von Willebrand Factor
D000941:Antigens; D014841:von Willebrand Factor; D003208:Concanavalin A; D005169:Factor VIII