Differences in modifications of cytoplasmic free Ca2+ concentration and 86Rb+ influx in human neoplastic B cells by antibodies to mu- relative to delta-Ig heavy chains.
Cytoplasmic free Ca2+ concentration and influx of 86Rb+ (K+ analogue) were determined during the first minutes after stimulation of neoplastic human B cells and B cell lines by antibodies to surface Ig. The Ca2+ concentration increased in the great majority of samples (41 of 48). All of four B cell lines also responded, providing formal evidence that accessory cells are not required for this early, surface Ig-mediated event. Antibodies to delta as well as mu, heavy chains (anti-delta and anti-mu) could induce both Ca2+ and 86Rb+ responses. 86Rb+ responders were found within the group of Ca2+ responders, but no quantitative relation was observed between the two responses. In cells expressing both sIgM and sIgD, antibodies to delta heavy chains were more potent than those to mu heavy chains in inducing Ca2+ responses, whereas the opposite pattern was seen with regard to 86Rb+ responses. These results demonstrate that sIgM and sIgD can deliver different biochemical signals to the cell.
D000912:Antibodies, Neoplasm / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007072:Immunoglobulin D / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D007138:Immunoglobulin delta-Chains / Q000276:immunology; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D007938:Leukemia / Q000276:immunology*; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000276:immunology*; D011868:Radioisotopes; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D012413:Rubidium / Q000378:metabolism*
D000912:Antibodies, Neoplasm; D007072:Immunoglobulin D; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007138:Immunoglobulin delta-Chains; D007148:Immunoglobulin mu-Chains; D011868:Radioisotopes; D011947:Receptors, Antigen, B-Cell; D012413:Rubidium; D002118:Calcium