Limited proteolysis of MP26 in lens fiber plasma membranes of the U18666A-induced cataract in rats.
Most of the animals treated with U18666A every other day beginning at one-day of age developed permanent nuclear cataracts by 3-4 weeks of age. Lens fiber plasma membranes were isolated from cortical and nuclear areas of untreated controls, treated but clear, and treated cataractous lenses, and analyzed by SDS-PAGE. MP26 was the major intrinsic polypeptide in the plasma membranes of both cortical and nuclear fibers of control lenses. MP26 was largely replaced by MP23-24 in the plasma membranes of nuclear fibers of treated but clear lenses, and in the membranes of both cortical and nuclear fibers of cataractous lenses.
D000736:Androstenes / Q000494:pharmacology*; D000818:Animals; D020346:Aquaporins; D002386:Cataract / Q000139:chemically induced* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D007908:Lens, Crystalline / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000736:Androstenes; D020346:Aquaporins; D005136:Eye Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C030328:aquaporin 0; C006261:3-beta-(2-(diethylamino)ethoxy)androst-5-en-17-one; D010447:Peptide Hydrolases