Association of nitrosamine-derived radioactivity with nuclear and mitochondrial DNA in mice.
In order to better understand the carcinogenic process, a comparative study was undertaken of the association of nitrosamine-derived radioactivity with nuclear and mitochondrial DNA isolated from tumor-susceptible and non-tumor-susceptible tissues of two strains of mice with different susceptibilities to tumor induction by dimethylnitrosamine (DMN) or diethylnitrosamine (DEN). A single dose of either [14C]-DMN or [14C]DEN was administered by intragastric intubation to young adult male RFM or BALB/c mice. The DNA isolated from the mitochondrial fraction of tumor-susceptible tissues bound several times (10 to 90) more nitrosamine than the nuclear DNA. These results suggest that mitochondrial DNA may be a target in the carcinogenic process induced by nitrosamine compounds.
D000818:Animals; D002250:Carbon Radioisotopes; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D004272:DNA, Mitochondrial / Q000378:metabolism*; D004052:Diethylnitrosamine / Q000378:metabolism*; D004128:Dimethylnitrosamine / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000378:metabolism; D009602:Nitrosamines / Q000378:metabolism*
D002250:Carbon Radioisotopes; D004272:DNA, Mitochondrial; D009602:Nitrosamines; D004052:Diethylnitrosamine; D004247:DNA; D004128:Dimethylnitrosamine