Fe3+ transport by brush-border membrane vesicles isolated from normal and hypoxic mouse duodenum and ileum.
Studies of 59Fe3+ uptake by brush-border membrane vesicles prepared from mouse duodenum have indicated that uptake represents transport across the brush-border membrane which is rate-limited by the membrane-transfer step (Simpson, R.J. and Peters, T.J. (1984) Biochim. Biophys. Acta 772, 220-226). Further studies presented here reveal that the uptake rate represents the net influx rate for Fe3+ and is independent of Na+ in the medium and of the method of vesicle preparation. Uptake by brush-border membrane vesicles prepared from mouse distal ileum also represents predominantly transport and is higher than that observed with duodenal brush-border membrane vesicles. Studies of the initial uptake rate by vesicles prepared from normal and hypoxic mouse intestine demonstrated an increase in Fe3+ transport in duodenal vesicles only.
D000818:Animals; D001693:Biological Transport, Active; D004386:Duodenum / Q000648:ultrastructure*; D005260:Female; D005290:Ferric Compounds / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D007082:Ileum / Q000648:ultrastructure*; D007501:Iron / Q000378:metabolism*; D008297:Male; D008353:Mannitol / Q000378:metabolism; D051379:Mice; D008871:Microvilli / Q000378:metabolism; D009994:Osmolar Concentration; D011247:Pregnancy; D012737:Sex Factors; D012964:Sodium / Q000378:metabolism
D005290:Ferric Compounds; D008353:Mannitol; D012964:Sodium; D007501:Iron