[Production of tetanolysin preparations and characteristics of their properties].
As the result of the study of tetanolysin-producing Clostridium tetani strains, their populations have been found to be markedly heterogeneous with respect to the hemolytic activity of clone cultures. On the basis of normal and dialyzed cultures of selected variants with maximum activity the preparations of tetanolysin have been obtained, and their hemolytic activity and antigenic properties have been studied. Antihemolytic rabbit sera have also been obtained and characterized. Partially purified preparations of tetanolysin with high hemolytic activity have been obtained by the fractionation of C. tetani dialyzed cultures with ammonium sulfate.
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000633:toxicity; D002417:Cattle; D002645:Chickens; D003017:Clostridium tetani / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006736:Horses; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D008297:Male; D011817:Rabbits; D012756:Sheep; D013744:Tetanus Toxin / Q000302:isolation & purification* / Q000633:toxicity; D013745:Tetanus Toxoid / Q000276:immunology
D001427:Bacterial Toxins; D003470:Culture Media; D007106:Immune Sera; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; C026167:tetanolysin
Poluchenie preparatov tetanolizina i kharakteristika ikh svoĭstv.