Cloning and expression of human interleukin 2 in Streptomyces lividans using the Escherichia coli consensus promoter.
Streptomyces lividans was transformed with a plasmid containing the structural gene that specifies human interleukin 2. The expression of interleukin 2 in this plasmid is controlled by both the consensus promoter and a consensus ribosome binding site characteristic of Escherichia coli. We have detected production of active human interleukin 2 in liquid cultures of the transformed Streptomyces by both, biological assay and immuno-blotting analysis.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D006801:Humans; D007120:Immunochemistry; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics*
D007376:Interleukin-2; D004247:DNA