The application of enzyme-linked immunosorbent assays (ELISA) to neuropeptides.
Procedures are presented for routine evaluation of antibody specificity, titre, and quantitation of antigen levels in tissue extracts without the use of radiolabeled probes. A colorimetric, enzyme-linked immunosorbent assay (ELISA) is described for general use with neuropeptides, using neurotensin as a primary example. These assays use rabbit anti-neurotensin immune serum which is colorimetrically identified after combination with an alkaline phosphatase-conjugated, affinity purified, goat anti-rabbit IgG and reaction with the chromogenic substrate, p-nitrophenyl phosphate. Because the principle of these methods can be adapted for use with various proteins and neuropeptides, they should find widespread applicability in neurobiology.
D000469:Alkaline Phosphatase; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D009496:Neurotensin / Q000032:analysis* / Q000276:immunology; D009596:Nitrophenols / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D013997:Time Factors
D007106:Immune Sera; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D009496:Neurotensin; D000469:Alkaline Phosphatase