Electrophoretic characterization of active renin from human kidney and inactive renin from a human chorionic cell culture.
Enzymatically inactive human renin from chorionic cells in culture is significantly distinct in polyacrylamide gel electrophoresis (pH 8.17, 0 degree C) from active human kidney renin. The inactive renin is larger and more basic than the active renin; their molecular weights derived from gel electrophoretic retardation coefficients relate as 47.5/35.3 kDa, their valences (net protons/molecule) as 2.14/1.85. In gel electrofocusing conducted in a mixture of simple buffers, both inactive and active renins exhibit 2 components at the steady-state. The molecular size and basicity of inactive renin are consistent with the hypothesis that it may be a precursor (prorenin), although the possibility that it is an inhibitor complex cannot be ruled out.
D002478:Cells, Cultured; D002823:Chorion / Q000201:enzymology*; D004586:Electrophoresis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007668:Kidney / Q000201:enzymology*; D008970:Molecular Weight; D011863:Radioimmunoassay; D012083:Renin / Q000032:analysis*
D012083:Renin