The nucleotide sequence of bacteriophage T5 leucine tRNA.
Uniformly 32P-labeled bacteriophage T5 leucine tRNA has been isolated by two-dimensional gel electrophoresis from phage-infected E. coli cells. Its nucleotide sequence has been determined by conventional techniques using TLC on cellulose for oligonucleotide fractionation: pGGGGCUAUGCUGGAACDGmGDAGACAAUACGGCCUUAGm6AU psi CCGUAGCUUAAAUGCGUGGGAGT psi CGAGUCUCCCUAGCCCCACCAoh. This tRNA has anticodon sequence UAG, which can presumably recognize all the four leucine-specific codons (CUN). The main feature of T5 tRNALeu is the absence of the A10-C25 and C31-psi 39 pairing in the D and anticodon stems, respectively.
D001483:Base Sequence; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000235:genetics; D009690:Nucleic Acid Conformation; D010761:Phosphorus Radioisotopes; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification; D013604:T-Phages / Q000235:genetics*
D010761:Phosphorus Radioisotopes; D012346:RNA, Transfer, Amino Acyl; D012367:RNA, Viral