Some properties of carbonic anhydrase from mineralizing hamster molars.
Carbonic anhydrase (CA) activity in mineralizing hamster molars was determined with a radiochemical assay. Hamster molar CA appeared to be very similar to CA from other sources. No pH optimum could be determined; the KM was 13.6 mM and CA was strongly inhibited by acetazolamide. EDTA and F- had almost no effect on the CA activity. This lack of fluoride effect suggests strongly that the protective effect of fluoride on dental mineral is not mediated by CA.
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D006224:Cricetinae; D004492:Edetic Acid / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D007700:Kinetics; D008647:Mesocricetus; D008903:Minerals / Q000378:metabolism*; D008963:Molar / Q000201:enzymology; D013997:Time Factors; D014070:Tooth / Q000201:enzymology / Q000378:metabolism*
D008903:Minerals; D004492:Edetic Acid; D002256:Carbonic Anhydrases; D000086:Acetazolamide; D005459:Fluorides