Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus.
Escherichia coli F-18 isolated from the feces of a healthy human is an excellent colonizer of the CD-1 mouse colon. In the present investigation, adhesion of E. coli F-18 to CD-1 mouse colonic mucus and bovine serum albumin (BSA), immobilized on polystyrene, was studied. Adhesion of E. coli F-18 to mucus was two- to sixfold greater than to either BSA or polystyrene. E. coli F-18 lipopolysaccharide specifically blocked adhesion of E. coli F-18 to mucus and mimicked adhesion of E. coli F-18 to mucus, BSA, and polystyrene. Purified capsule also blocked adhesion of E. coli F-18 to mucus, but this inhibition was found to be entirely nonspecific. The specific E. coli F-18 receptor in mucus appeared to be a glycoprotein, containing sugars normally found in mucins and having a maximum molecular weight of between 1.25 X 10(5) and 2.5 X 10(5).
D000268:Adhesiveness; D000818:Animals; D003106:Colon / Q000382:microbiology*; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000502:physiology*; D005243:Feces / Q000382:microbiology*; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009093:Mucus / Q000032:analysis / Q000382:microbiology*
D006023:Glycoproteins; D008070:Lipopolysaccharides; D004492:Edetic Acid