An adoptive transfer system for the investigation of granuloma formation in murine listeriosis.
A system is described for studying adoptive transfer of granuloma formation in infection of mice with the facultative intracellular bacterium, Listeria monocytogenes. Intravenous injection of graded numbers of L. monocytogenes-immune peritoneal exudate T-enriched cells (PETLEs) together with 5 X 10(4) living L. monocytogenes resulted in dose-dependent accelerated granuloma formation in the livers of recipient mice. The lymphoid cells conferring granuloma formation were T cells by virtue of their nonadherence to nylon wool and sensitivity to anti-Thy 1.2 antiserum plus complement. Since granuloma formation could not be transferred from C57B1/6J donors to BALB/c recepients it is concluded that adoptive transfer of granuloma formation is genetically restricted.
D000818:Animals; D005122:Exudates and Transudates / Q000276:immunology; D006099:Granuloma / Q000276:immunology; D007116:Immunization, Passive; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors