Anomeric specificity of glucose metabolism in the pentose cycle.
The production of 3H2O from alpha- and beta-D-[5-3H]glucose and that of 14CO2 from either alpha- and beta-D-[1-14C] or alpha- and beta-D-[6-14C]glucose were measured in rat pancreatic islets and tumoral insulin-producing cells incubated at 7 degrees C. The ratio in 14CO2 output from D-[1-14C]glucose/D-[6-14C]glucose, the fraction of glucose metabolism occurring through the pentose cycle, and the flow rate through such a cycle were always higher in the presence of beta- than alpha-D-glucose. This indicates that the anomeric specificity of glucose-6-phosphate dehydrogenase is operative in intact islet cells.
D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D066298:In Vitro Techniques; D007340:Insulinoma / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D010084:Oxidation-Reduction; D010190:Pancreatic Neoplasms / Q000378:metabolism; D010429:Pentoses / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D014316:Tritium
D002250:Carbon Radioisotopes; D010429:Pentoses; D014316:Tritium; D005947:Glucose