Morphological and functional investigations on macrophages with heterogeneous fluorescent staining.
Four heterogeneous colors of fluorescence were demonstrated in macrophages from rabbit lymph nodes and mouse peritoneal exudate using fluorescent staining. They were bright blue, light blue, bluish green and yellow colors of fluorescence respectively. All of these 4 types of macrophages with heterogeneous colors of fluorescence could form EA or EAC rosettes and were able to phagocytose chicken erythrocytes and ink carbon granules. Among them the macrophages with bright blue color of fluorescence could form large EA or EAC rosettes and showed more rapid and stronger activity to phagocytose chicken erythrocytes, while those with bluish green and yellow colors of fluorescence showed stronger activity to phagocytose ink carbon granules.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008297:Male; D051379:Mice; D010587:Phagocytosis; D011817:Rabbits; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
D005456:Fluorescent Dyes; D000949:Histocompatibility Antigens Class II