Mutagenesis of bacteriophage T7 and T7 DNA by alkylation damage.
We have developed a new assay for in vitro mutagenesis of bacteriophage T7 DNA that measures the generation of mutations in the specific T7 gene that codes for the phage ligase. This assay was used to examine mutagenesis caused by in vitro DNA synthesis in the presence of O6-methylguanosine triphosphate. Reversion of one of the newly generated ligase mutants by ethyl methanesulfonate was also tested.
D000478:Alkylation; D011088:DNA Ligases / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005020:Ethyl Methanesulfonate; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives; D009024:Morphogenesis; D009152:Mutagenicity Tests; D009154:Mutation; D011118:Polynucleotide Ligases / Q000235:genetics*; D013604:T-Phages / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins; D006160:Guanosine Triphosphate; D005020:Ethyl Methanesulfonate; D011088:DNA Ligases; D011118:Polynucleotide Ligases