Polyclonal B cell stimulation and interleukin 1 induction by the mucoid exopolysaccharide of Pseudomonas aeruginosa associated with cystic fibrosis.
Mucoid exopolysaccharide isolated from Pseudomonas aeruginosa obtained from colonized cystic fibrosis patients was found to be a potent mitogen for mouse lymphocytes. The responding lymphocyte was a B cell, and we found no evidence that T cell could proliferate or synergize with B cells in response to the mucoid exopolysaccharide. Proliferation was not inhibitable by polymyxin B, which blocks lipopolysaccharide (LPS)-induced proliferation, indicating that a minor LPS contaminant in the purified exopolysaccharide was not the mitogenic component. Mucoid exopolysaccharide induced secretion of IgG, suggesting that it is polyclonal mitogen. It also induced splenic adherent cells (macrophages) to produce interleukin 1. We propose that mucoid exopolysaccharide produced by P. aeruginosa present in the lungs of cystic fibrosis patients may have potent in vivo consequences resulting in aberrant immunoregulation and inhibition of effective immune elimination of P. aeruginosa.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D003550:Cystic Fibrosis / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000276:immunology; D013154:Spleen / Q000166:cytology
D007136:Immunoglobulins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008934:Mitogens; D011135:Polysaccharides, Bacterial