Isolation of morphine from toad skin.
A nonpeptide opioid was found in toad skin and purified to homogeneity by using HPLC with electrochemical detection. A nonpeptide opioid also was detected in bovine brain and adrenals as well as rabbit and rat skin, by reversed-phase HPLC following Sephadex G-15 column chromatography. The material in toad skin was identical to morphine by immunological, pharmacological, and physical chemical criteria.
D000311:Adrenal Glands / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002024:Bufo marinus / Q000378:metabolism*; D002417:Cattle; D009020:Morphine / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats; D012867:Skin / Q000032:analysis*; D013045:Species Specificity; D014018:Tissue Distribution
D009020:Morphine