Comparison of two immuno-mechanical methods of T-cell depletion of human bone-marrow for prevention of graft-versus-host disease: soybean lectin agglutination and sheep erythrocyte rosette depletion versus triple rosette depletion.
Depletion of T-lymphocytes from HLA-mismatched donor bone marrow can be accomplished by either triple neuraminidase-treated sheep erythrocyte depletion (3En) or soybean agglutination and sheep erythrocyte depletion (SBA/E/En). T-lymphocyte depletion by 3En resulted in higher yields of all marrow precursor phenotypes (studied by a battery of monoclonal antibodies) than did SBA/E/En depletion. While both procedures enriched for some early precursor cells (e.g., HLA-DR and/or terminal deoxynucleotidyl transferase (TdT) positive cells), greater numbers of these cells were available after 3En. Clinical benefits which may be derived from the larger inoculum of T-depleted bone-marrow cells available after 3En treatment remain to be studied.
D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002469:Cell Separation / Q000379:methods; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D004912:Erythrocytes; D006086:Graft vs Host Disease / Q000517:prevention & control*; D006801:Humans; D037102:Lectins; D037121:Plant Lectins; D012397:Rosette Formation; D012756:Sheep; D030262:Soybean Proteins; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D010835:Phytohemagglutinins
D000911:Antibodies, Monoclonal; D004253:DNA Nucleotidylexotransferase; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins