An improved method for electron microscopic observation of cerebrospinal fluid cells.
Human cerebrospinal fluid (CSF) cells were entrapped between two bovine serum albumin cylinders linked by glutaraldehyde using microhematocrit tubes. The "sandwiched" specimen could be processed in further preparatory steps like tissue for routine electron microscopy. This rapid procedure resulted in adequate cell preservation for transmission electron microscopy without substantial loss of CSF cells.
D002555:Cerebrospinal Fluid / Q000166:cytology*; D005404:Fixatives; D005976:Glutaral / Q000008:administration & dosage; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*
D005404:Fixatives; D005976:Glutaral