[Antibody formation to o-aminoazotoluene via the administration of conjugated antigens synthesized by enzymatic and chemical methods].
Two new methods are developed for synthesis of conjugated antigens of o-aminoazotoluene-bovine serum albumin (o-AAT--BSA): (1) from the microsomal fraction of the guinea pig liver which contains the cytochrome-P-450-dependent monooxygenase enzymic system and (2) from the m-chloroperbenzoic acid. A possible mechanism of the covalent binding of o-AAT with albumin under the effect of monooxigenases and of their chemical model is considered. Differences of antigenic determinants in conjugated antigens of o-AAT--BSA synthetized by the chemical and enzymic methods are detected.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000138:chemical synthesis / Q000276:immunology*; D001391:Azo Compounds / Q000276:immunology*; D002417:Cattle; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods; D007158:Immunologic Techniques; D008862:Microsomes, Liver / Q000201:enzymology / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D013997:Time Factors; D009762:o-Aminoazotoluene / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D001391:Azo Compounds; D012710:Serum Albumin, Bovine; D003577:Cytochrome P-450 Enzyme System; D009762:o-Aminoazotoluene
Obrazovanie antitel k o-aminoazotoluolu pri vvedenii kon''iugirovannykh antigenov, sintezirovannykh fermentativnymi i khimicheskimi metodami.