Transmission and expression of a specific pair of rearranged immunoglobulin mu and kappa genes in a transgenic mouse line.
Two genes that code for a hapten-specific immunoglobulin M (IgM) have been introduced into the mouse germ line. The transgenic antibody represents 10-50% of the serum IgM and is expressed on the membrane of B cells. B-cell hybridoma lines show that a negative feedback inhibition of mu and kappa transgenic products on the immunoglobulin heavy-chain rearrangement is possible.
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005796:Genes; D006825:Hybridomas / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D051379:Mice; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic
D004274:DNA, Recombinant; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007148:Immunoglobulin mu-Chains; D012333:RNA, Messenger