Neonatal treatment with nerve growth factor antiserum eliminates cholinergic sympathetic innervation of rat sweat glands.
Most mammalian sympathetic neurons are noradrenergic, and their dependence upon nerve growth factor (NGF) for survival during development is well established. A minor population of sympathetic neurons, including those that innervate sweat glands, is cholinergic. To determine whether cholinergic sympathetic neurons, like their noradrenergic counterparts, require NGF during development, neonatal rats were treated with NGF-antiserum and 3 weeks later their sweat glands were examined for the presence of innervation. Acetylcholinesterase (AChE) staining and vasoactive intestinal polypeptide-like immunoreactivity (VIP-IR) which mark the mature sweat gland innervation were absent from the sweat glands of the anti-NGF treated animals. Further, when the glands were examined with the electron microscope, no axons or nerve terminals were evident. These observations indicate that the elaboration of the sweat gland plexus is NGF-dependent and suggest that at least one population of cholinergic sympathetic neurons in the rat requires NGF for survival. Our findings are consistent with the idea that during development NGF is a required trophic factor not only for noradrenergic sympathetic but also for cholinergic sympathetic neurons.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development*; D002799:Cholinergic Fibers / Q000254:growth & development*; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D009414:Nerve Growth Factors / Q000276:immunology / Q000502:physiology*; D051381:Rats; D013545:Sweat Glands / Q000294:innervation*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D009414:Nerve Growth Factors; D014660:Vasoactive Intestinal Peptide; D000110:Acetylcholinesterase