Comparison of the immunochemical responses by anti-NADPH-cytochrome c reductase of Tetrahymena pyriformis (strain NT-1) in protozoan cells and mammalian liver microsomes.
Comparison of the microsomal NADPH-cytochrome c reductase activities in the four Tetrahymena cells (pyriformis, strain GL and NT-1; thermophilia; ISO) and rat liver was studied. The reductase activity in strain NT-1 was lowest among four Tetrahymena cells grown at 24 degrees C. Rabbit antibody was prepared against the purified NADPH-cytochrome c reductase from Tetrahymena pyriformis (strain NT-1) microsomes. Microsomal NADPH-cytochrome c reductase activities in various Tetrahymena cells were inhibited in proportion to the amount of antibody added, in the order of GL greater than NT-1 greater than thermophilia greater than ISO. No inhibition of reductase activity by antibody was observed in rat liver microsomes.
D000818:Animals; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D008862:Microsomes, Liver / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity; D013768:Tetrahymena / Q000201:enzymology*; D013769:Tetrahymena pyriformis / Q000201:enzymology*
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D009251:NADPH-Ferrihemoprotein Reductase