Large-scale clinical comparison of the lysis-centrifugation and radiometric systems for blood culture.
The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with the BACTEC radiometric method (Johnston Laboratories, Inc., Towson, Md.) with 6B and 7D broth media for the recovery of bacteria and yeasts. From 11,000 blood cultures, 1,174 clinically significant organisms were isolated. The Isolator system recovered significantly more total organisms, members of the family Enterobacteriaceae, Staphylococcus spp., and yeasts. The BACTEC system recovered significantly more Pseudomonas spp., Streptococcus spp., and anaerobes. Of the Isolator colony counts, 87% measured less than 11 CFU/ml of blood. Organisms, on an average, were detected the same day from each of the two culture systems. Only 13 of the 975 BACTEC isolates (0.01%) were recovered by subculture of growth-index-negative bottles, and 12 of the 13 were detected in another broth blood culture taken within 24 h. Contaminants were recovered from 4.8% of the Isolator 10 and 2.3% of the BACTEC cultures.
D000328:Adult; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001769:Blood / Q000382:microbiology*; D002498:Centrifugation; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006461:Hemolysis; D006801:Humans; D008828:Microbiological Techniques; D011549:Pseudomonas / Q000302:isolation & purification; D011874:Radiometry; D018805:Sepsis / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000302:isolation & purification; D013291:Streptococcus / Q000302:isolation & purification; D015003:Yeasts / Q000302:isolation & purification