A selenium-containing nucleoside at the first position of the anticodon in seleno-tRNAGlu from Clostridium sticklandii.
In previous studies, the single selenonucleoside component of a selenium-containing tRNAGlu isolated from Clostridium sticklandii has been shown to be 5-methyl-aminomethyl-2-selenouridine. Here, we show that this selenonucleoside is most likely located at the "wobble" position of the anticodon of the clostridial seleno-tRNAGlu. Nuclease T1 digestion of this seleno-tRNAGlu generated one major selenium-containing oligonucleotide (25 bases long). The selenium-containing residue within this oligonucleotide was located by sequence analysis of the oligonucleotide before and after removal of selenium by treatment with cyanogen bromide. The sequence of this oligonucleotide, A-A-C-C-G-C-C-C-U-U+-U-C-A+C-G-G-C-G-G-U-A-A-C-A-G, is homologous to that of the Escherichia coli tRNAGlu2 from residues 27 to 50, including the anticodon region and the variable loop, except that the E. coli tRNA has 5-methylaminomethyl-2-thiouridine instead of the selenonucleoside.
D000926:Anticodon / Q000032:analysis*; D001483:Base Sequence; D003013:Clostridium / Q000235:genetics*; D003488:Cyanogen Bromide / Q000494:pharmacology; D009841:Oligonucleotides / Q000032:analysis; D016566:Organoselenium Compounds; D012343:RNA, Transfer / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*; D006163:Ribonuclease T1 / Q000378:metabolism; D012643:Selenium / Q000032:analysis*; D014529:Uridine / Q000031:analogs & derivatives / Q000032:analysis
D000926:Anticodon; D009841:Oligonucleotides; D016566:Organoselenium Compounds; D012346:RNA, Transfer, Amino Acyl; C366009:seleno-tRNA; C043608:5-((methylamino)methyl)-2-selenouridine; D012343:RNA, Transfer; D006163:Ribonuclease T1; D012643:Selenium; D003488:Cyanogen Bromide; D014529:Uridine