[Prospective use of immunofluorescence polarization in the study of enzymes modifying aminoglycosides].
A number of assays have been described for aminoglycoside-modifying enzymes; the aim of our investigation was to check the validity of polarized immunofluorescence assay for aminoglycoside enzymes. We have studied three enzymes encoded by R-factors from clinical isolates of Enterobacteriaceae conferring various patterns of aminoglycoside-resistance. The assay resulted valid: this is a simple, rapid and sensitive method.
D000617:Aminoglycosides / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology*; D005454:Fluorescence Polarization; D007158:Immunologic Techniques; D011815:R Factors
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents
Prospettive di utilizzazione della immunofluorescenza polarizzata per lo studio degli enzimi modificanti gli aminoglicosidi.