Mutagenic metabolites in rat urine after dermal exposure to 1,3-diaminobenzene.
Amberlite XAD-2 purified urine extracts from rats treated dermally with 1,3-diaminobenzene were fractionated by HPLC and the metabolites were identified by GC-MS. Mutagenic activity in Salmonella typhimurium TA 98 was equally distributed between the two fractions, none of which contained the parent amine. The first fraction, eluted between 2-7 minutes, contained only a very small amount of presently unidentified metabolites, while the second fraction eluted between 14-19 minutes contained N,N'-diacetyl-1,3-diaminobenzene. Both fractions showed minor, but still significant activity even without metabolic activation. A non-mutagenic fraction, eluted between 10-12 minutes contained N-acetyl -acetyl-1,3-diaminobenzene and N,N'-diacetyl-2,4-diaminophenol.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000652:urine*; D010655:Phenylenediamines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects
D009153:Mutagens; D010655:Phenylenediamines; C008381:3-phenylenediamine