Characterization of a monoclonal antibody to myosin specific for mammalian and human type II muscle fibers.
We have characterized a monoclonal antibody (McAb), ALD-19, generated against slow myosin from chicken anterior latissimus dorsi (ALD) muscle for use in studies of human and animal muscle fiber types. This McAb bound selectively to the 200 kDa myosin heavy chain band in immunoblots against chicken, rat and human myosins and showed selective staining of A bands in the myofibrils. The reactivity of ALD-19 with various myosin types was quantitated by radioimmunoassays. Fiber type analysis revealed unexpected specificity of McAb ALD-19 for type II mammalian muscle fibers. This antibody should, therefore be useful for identification and quantification of normal type II fibers in human muscle biopsy specimens.
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D005455:Fluorescent Antibody Technique; D006801:Humans; D009132:Muscles / Q000032:analysis / Q000166:cytology / Q000276:immunology*; D009218:Myosins / Q000276:immunology* / Q000302:isolation & purification; D051381:Rats; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D009218:Myosins