[Immunochemical identification and study of an interspecific thymus antigen (AgT-1)].
A new interspecific human and animal thymic antigen (AgT-1) was identified immunochemically. It was shown that AgT-1 is a protein with a molecular mass about 40000 dalton, electrophoretic mobility of alpha 1-globulins and isoelectric points 4.0 and 4.5. Heating of the protein to 80 degrees C led to the loss of its immunochemical activity. Antisera to AgT-1 were obtained by immunization of rabbits by conjugated extract of bovine thymus in complete Freund's adjuvant. AgT-1 was identified immunochemically in bovine embryonal thymus, spleen and liver. In addition to these organs, AgT-1 was discovered in lung extracts of adult animals. Identical antigen was identified in the embryonal thymus and extracts of human small intestine. AgT-1 antibodies inhibited the biological activity of the active thymic fraction (AFT-6) in recovering the sensitivity of spleen fRFC from thymectomized mice to the inhibitory action of azathioprin. The data indicate that the biological activity of AFT-6 is partly due to the molecules having antigenic determinants of AgT-1.
D000328:Adult; D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004587:Electrophoresis, Agar Gel; D005333:Fetus / Q000276:immunology; D006801:Humans; D007114:Immunization; D007120:Immunochemistry; D005779:Immunodiffusion; D007526:Isoelectric Point; D051379:Mice; D011817:Rabbits; D012397:Rosette Formation; D013045:Species Specificity; D013950:Thymus Gland / Q000276:immunology*; D013997:Time Factors
D000941:Antigens
Immunokhimicheskaia identifikatsiia i izuchenie mezhvidovogo antigena timusa (AgT-1).