Change in the physical state of platelet plasma membranes upon ionophore A23187 activation. A fluorescence polarization study.
Human platelets were isolated and fluorescence-labelled by 1,6-diphenylhexatriene. Diphenylhexatriene was essentially localized in the plasma membrane, as indicated by trinitrobenzenesulfonate-quenching experiments. A decrease of the fluorescence polarization of diphenylhexatriene was observed upon ionophore A23187 addition in the absence of aggregation. 0.3 microM ionophore allowed to reach the maximum rate of the decrease of fluorescence polarization; it also maximally stimulated the light transmission change, the serotonin release and the thromboxane B2 synthesis. The amplitude of the fluorescence polarization decrease was maximum at platelet concentrations between 4 X 10(7) and 7 X 10(7)/ml. The presence of Ca2+ in the medium increased the rate constant of the polarization change. Chlorpromazine (60 microM) completely inhibited this transition, but at 30 microM its inhibitory effect was reversed by Ca2+. The membrane events implied in platelet activation very likely lead to fluidization of the plasma membrane, perhaps by its fusion with the membranes of internal granules which are relatively depleted of cholesterol. Ca2+ plays a central role in the triggering of the observed effects at the membrane level.
D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects*; D002746:Chlorpromazine / Q000494:pharmacology; D004161:Diphenylhexatriene / Q000032:analysis; D005454:Fluorescence Polarization; D006801:Humans
D004161:Diphenylhexatriene; D000001:Calcimycin; D002122:Calcium Chloride; D002746:Chlorpromazine