The utility of the macrophage migration inhibition test for in vitro titration of tuberculins.
The purpose of this study was to explore the fundamental principle of the potency estimation of tuberculins, as applied in vitro by the macrophage migration inhibition test (MIT) under agarose. The MIT was performed using specifically sensitized mouse and guinea-pig peritoneal macrophages and serial dilutions of the analogous PPD (purified protein derivatives of tuberculin) tuberculins as antigen. Statistical studies performed included (a) the standard deviation of the mean migration areas, (b) the analysis of variance, (c) the regression analysis, (d) its corresponding linearity test and (e) the determination of the related correlation coefficient. It was shown for the first time and in both animal species under study that there is correspondence between the log dose-response relationship of the tuberculin PPD in MIT under agarose and the well known in tuberculin cutaneous reaction. The MIT may therefore successfully replace the in vivo titration of tuberculins.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D002464:Cell Migration Inhibition; D007114:Immunization; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008954:Models, Biological; D009163:Mycobacterium bovis / Q000276:immunology; D014373:Tuberculin / Q000032:analysis*
D000942:Antigens, Bacterial; D014373:Tuberculin