Oral colonization and susceptibility testing of Pseudomonas aeruginosa oral isolates from cystic fibrosis patients.
Microbial samples from the oral cavities of cystic fibrosis (C.F.) patients and 20 age-matched normal control subjects were characterized. Mucoid variant Pseudomonas aeruginosa was isolated from the tongue, buccal mucosa, and saliva of C.F. patients only. Analysis of the data suggests that the oral cavity is a potential reservoir for this organism. Aspiration and cross-contamination from this reservoir may be important in perpetuating chronic pulmonary infection in C.F. patients. Susceptibility testing was performed on 20 mucoid variant P. aeruginosa oral isolates obtained from the patients according to standardized broth dilution procedures. The in vitro antimicrobial effects of sodium fluoride, stannous fluoride, and chlorhexidine were measured. Analysis of the data suggests that clinically safe and achievable levels of chlorhexidine and stannous fluoride may be antimicrobial.
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002710:Chlorhexidine / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000302:isolation & purification*; D012463:Saliva / Q000382:microbiology; D012969:Sodium Fluoride / Q000494:pharmacology; D014002:Tin Fluorides / Q000494:pharmacology; D014059:Tongue / Q000382:microbiology
D014002:Tin Fluorides; D012969:Sodium Fluoride; D002710:Chlorhexidine