Differential effect of dichloroacetate on branched-chain amino acid catabolism in perfused rat hindlimbs.
The effect of 1 mM and 5 mM dichloroacetate on the catabolism of branched-chain amino acids in isolated rat hindlimbs was investigated in perfusions with 0.5 mM 1-14C-labeled L-leucine or L-valine. The results demonstrate an increasing effect of dichloroacetate on the flux through skeletal muscle branched-chain 2-oxo acid dehydrogenase. A minor effect was observed with the high dichloroacetate concentration. Evidence is presented that this was essentially due to diminished pyruvate supply.
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000085:Acetates / Q000494:pharmacology*; D000597:Amino Acids, Branched-Chain / Q000378:metabolism*; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D006614:Hindlimb; D007658:Ketone Oxidoreductases / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D014633:Valine / Q000378:metabolism
D000085:Acetates; D000597:Amino Acids, Branched-Chain; D009097:Multienzyme Complexes; D011773:Pyruvates; D002245:Carbon Dioxide; D019289:Pyruvic Acid; D003999:Dichloroacetic Acid; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D007930:Leucine; D014633:Valine