In vitro T cell-mediated killing of Pseudomonas aeruginosa. II. The role of macrophages and T cell subsets in T cell killing.
T lymphocytes from immune mice can adoptively transfer protection against infection with the extra-cellular Gram-negative bacterium Pseudomonas aeruginosa to nonimmune recipients, and in vitro, immune T cells are able to kill these bacteria. Earlier studies indicated that this killing is mediated by a bactericidal lymphokine. Those studies also showed that macrophages enhance this in vitro T cell killing but do not directly participate in the bacterial killing, nor do macrophages function to present antigen to T cells. The current studies demonstrate that the ability of macrophages to enhance T cell killing can be replaced by macrophage culture supernatants or by purified recombinant interleukin 1 (IL 1). In addition, the macrophage supernatant-induced enhancement can also be blocked by antibody to purified IL 1. These studies also demonstrate that the T cell subset that serves as the final effector cell in the killing process is the Lyt-1-, 2,3+, I-J+ phenotype.
D000818:Animals; D001667:Binding, Competitive; D002450:Cell Communication; D005260:Female; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D007074:Immunoglobulin G / Q000502:physiology; D007375:Interleukin-1 / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology* / Q000502:physiology
D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D007074:Immunoglobulin G; D007375:Interleukin-1