The complete amino acid sequence of the major mammalian neurofilament protein (NF-L).
The first complete amino acid sequence of a neurofilament protein has been established. Porcine NF-L contains 548 residues corresponding to a molecular mass of approximately 62 kDa. This value is noticeably smaller than the 68-72 kDa estimates from gel electrophoresis. Sequence comparison among the 6 non-epithelial intermediate filament (IF) proteins of warm-blooded vertebrates shows that the three NF proteins are the most remote members. Additionally and unexpectedly they reveal among each other lower sequence identity than the three non-neuronal IF proteins GFAP, desmin, and vimentin where the last two are particularly closely related. Certain schemes of IF protein evolution are discussed.
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D006224:Cricetinae; D003893:Desmin; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D051379:Mice; D016900:Neurofilament Proteins; D010446:Peptide Fragments; D013116:Spinal Cord / Q000032:analysis; D013552:Swine; D014746:Vimentin
D003893:Desmin; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D010446:Peptide Fragments; D014746:Vimentin; C044882:neurofilament protein L