[Differential affinity of pathogenic species of microorganisms for a set of lectins detectable by the sandwich method using fluorescein isothiocyanate (FITC)].
The qualitative differences in the affinity of concanavalin A (Con A), wheat-germ agglutinin (WGA) and Phaseolus vulgaris lectin to the surface of 10 microbial strains inducing various diseases in humans and agricultural animals have been demonstrated by means of the indirect immunofluorescence tests. Enterobacteria, Coxiella burnetii and Bacillus anthracis have been found to possess pronounced affinity to Con A and WGA, while Rickettsia prowazekii, Francisella tularensis and Brucella abortus, as well as Treponema pallidum, have proved to be resistant to lectins. WGA has been found to bind specifically to Brucella abortus and Treponema pallidum. Con A and WGA are seemingly suitable for use in the preliminary test for the total capacity of lectin receptors to come in contact with biological macromolecules.
D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D003208:Concanavalin A / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000494:pharmacology*; D007114:Immunization; D007117:Immunization, Secondary; D037102:Lectins / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011817:Rabbits; D013861:Thiocyanates / Q000494:pharmacology*; D014909:Wheat Germ Agglutinins
D005452:Fluoresceins; D005456:Fluorescent Dyes; D037102:Lectins; D010835:Phytohemagglutinins; D013861:Thiocyanates; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate
Differentsirovannoe srodstvo patogennykh vidov mikroorganizmov k naboru lektinov, proiavliaemykh "séndvich"-metodom s uchastiem fliuorestseinizotiotsionata (FITTs).