The synthesis of lipids from [1-14C]acetate by isolated rat brain capillaries.
Lipid biosynthesis was investigated in isolated cerebral microvessels obtained from adult Sprague-Dawley rats using [1-14C]acetate as precursor. All lipid classes were labelled by [1-14C]acetate. Neutral lipids incorporated about 50% of radiolabelled acetate, among which free fatty acids and triglycerids showed the highest level of incorporation. Moreover, about 4% of radioactivity was found in cholesterol fraction. In phospholipid fraction, phosphatidylcholine and phosphatidylethanolamine were the main radiolabelled phospholipids. [1-14C]acetate was also incorporated into sulphatides and cerebrosides. The presence of bovine serum albumin in incubation medium modified the percentage of incorporation in different lipid fractions.
D000085:Acetates / Q000378:metabolism*; D000818:Animals; D002196:Capillaries / Q000033:anatomy & histology / Q000378:metabolism; D002250:Carbon Radioisotopes; D002560:Cerebrovascular Circulation; D005260:Female; D006017:Glycolipids / Q000096:biosynthesis; D066298:In Vitro Techniques; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D008722:Methods; D008858:Microscopy, Phase-Contrast; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D002250:Carbon Radioisotopes; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids