Nitroarenes in Suimon River sediment.
Mutagenic activity was observed in sediments of the Suimon River bed with and without S9 mix. The direct-acting mutagens in the sediment were investigated. The sediment was extracted with methanol and fractionated on a Silica gel column. The benzene fraction from the Silica gel column exhibited mutagenic activity without S9 mix in strain TA98, while it failed to show mutagenic activity in nitroreductase-deficient strain TA98NR. This observation led to the suspicion that nitro compounds were the direct-acting mutagens of these samples. The benzene fraction was treated by heptafluorobutyric anhydride (HFBA) and investigated with gas chromatography equipped with an electron capture detector (GC-ECD). 2-Nitrofluorene, 4,4'-dinitrobiphenyl, 2,7-dinitrofluorene and 1-nitropyrene were detected and measured quantitatively. The mutagenic activity of a mixture of these compounds was compared with that of the original fraction and the direct-acting mutagenicity of Suimon River sediment can be explained by these nitroarenes, especially 1-nitropyrene.
D000818:Animals; D007564:Japan; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D009574:Nitro Compounds / Q000633:toxicity*; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects; D012989:Soil Pollutants / Q000302:isolation & purification / Q000633:toxicity; D013329:Structure-Activity Relationship; D014873:Water Pollutants / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000633:toxicity*
D009153:Mutagens; D009574:Nitro Compounds; D012989:Soil Pollutants; D014873:Water Pollutants; D014874:Water Pollutants, Chemical