Immunohistochemical studies with antibodies to myosins from the cytoplasm and membrane fraction of human blood platelets.
Antibodies were raised to myosins extracted from the cytoplasm and solubilized membranes of human blood platelets. Both antibodies had similar titers as shown by enzyme-immunoassay and bound to the same sites as shown by immunohistochemistry. They were specific for cytoplasmic myosins (e.g., in human white blood cells, platelets and fibroblasts and rat endothelial cells). They showed no crossreaction with human or rat smooth muscle.
D000818:Animals; D000906:Antibodies; D001792:Blood Platelets / Q000166:cytology*; D002462:Cell Membrane / Q000032:analysis; D002645:Chickens; D003593:Cytoplasm / Q000032:analysis; D005260:Female; D005895:Gizzard, Avian / Q000166:cytology; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000166:cytology; D009130:Muscle, Smooth / Q000166:cytology; D009132:Muscles / Q000166:cytology; D009206:Myocardium / Q000166:cytology; D009218:Myosins / Q000032:analysis / Q000097:blood*; D009928:Organ Specificity; D011247:Pregnancy; D051381:Rats; D013045:Species Specificity
D000906:Antibodies; D009218:Myosins