Immunohistochemical analysis of the cross-reaction of anti-rat histidine decarboxylase antibody with guinea-pig DOPA decarboxylase.
L-Histidine decarboxylase [L-histidine carboxylyase, HDC, EC 4.1.1.22] is an enzyme distinct from L-DOPA decarboxylase [L-aromatic amino acid carboxylyase, DDC, EC 4.1.1.28]: the two decarboxylases from fetal rat liver were completely separated from each other by DEAE-cellulose column chromatography and by affinity chromatography with L-carnosine as a ligand. The antibody raised against this HDC inhibited the HDC's from rat and guinea-pig brains very strongly, but their DDCs very weakly. However, in immunofluorescent histochemical studies, the antibody cross-reacted with DDC-like immunoreactive structures, such as chromaffin cells of the adrenal medulla, the raphe nucleus, the substantia nigra, and the locus coeruleus of the brain of guinea-pigs, but not of rats, suggesting that these two decarboxylases share some antigenic structures.
D000313:Adrenal Medulla / Q000201:enzymology; D000818:Animals; D000906:Antibodies / Q000502:physiology; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000276:immunology*; D001667:Binding, Competitive; D001921:Brain / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000276:immunology*; D003429:Cross Reactions; D004296:Dopa Decarboxylase / Q000276:immunology* / Q000302:isolation & purification; D005333:Fetus; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000302:isolation & purification; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D002262:Carboxy-Lyases; D004296:Dopa Decarboxylase; D006640:Histidine Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases