The pipette-method: its application to cytogenetic studies of tumor cells cloned in semisolid media.
A simple method is herein described which allows easy cytogenetic analyses of human tumor cells grown in semisolid media. The quality of the metaphases is relatively good, comparable with that reached by using conventional cytogenetic methods. Therefore, the demonstration of G-, Q-, C-bands, and nucleolus organizing regions (NOR) is possible without any problems. Its particular advantage, however, lies in the fact that single colonies can be removed under sterile conditions at different stages of cell culturing.
D002460:Cell Line; D002871:Chromosome Banding; D002877:Chromosomes, Human; D005821:Genetic Techniques; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D014410:Tumor Stem Cell Assay / Q000379:methods