[Immobilization of proteolytic complexes on pectin-containing carriers].
A number of sorbents were synthesized on the basis of pectin and then used for immobilization of proteolytic complexes--pancreatin and protosubtilin. The best properties were shown by the enzyme preparations based on pectin, formaldehyde and melamin (PFM). Thus immobilization of pancreatin on PFM through Fe(III) ions gave a preparation with the activity of 79 000 mumole/g X h with respect to methyl ester of L-tryptophane (the activity yield is 91%). The pH optimum for all immobilized preparations was shifted towards the alkaline region. The thermostable fraction of the immobilized preparations retains the activity at 60 degrees for a long time.
D001412:Bacillus subtilis / Q000201:enzymology; D004792:Enzyme Precursors / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005557:Formaldehyde / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010194:Pancreatin / Q000378:metabolism; D010368:Pectins / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D014227:Triazines / Q000378:metabolism
D004792:Enzyme Precursors; D004800:Enzymes, Immobilized; D014227:Triazines; D005557:Formaldehyde; D010194:Pancreatin; D010368:Pectins; C040620:protosubtilin; D010447:Peptide Hydrolases; C011907:melamine
Immobilizatsiia proteoliticheskikh kompleksov na pektinsoderzhashchikh nositeliakh.