[Increased transformation ability of Escherichia coli strains carrying the plasmid pLD4].
The effect of resident plasmid pLD4, a derivative of plasmid Hly241, on transformability of the host bacteria cells has been studied. Plasmid pLD4 was transferred into the different strains of E. coli subsequently transformed by the DNA of plasmids pBR322, pBR325, pAL-R2, pMB9. The majority of strains harbouring pLD4 obtain the increased ability to be transformed as compared with the ability of isogenic plasmidless strains. The similar but less expressed effect was conferred by the plasmid Hly241. Another hemolytic plasmid Hly195 and its derivatives, carrying the different transposons, as well as plasmid F' tet Hly did not increase the transformability of host bacteria. The optimal parameters for transformation of the strains harbouring pLD4 and plasmidless strains coincide, but the number of competent cells is considerably higher for plasmid containing strains, due to preliminary results.
D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D013045:Species Specificity; D014169:Transformation, Bacterial
Povyshennaia sposobnost' k transformatsii shtammov kishechnoĭ palochki s plazmidoĭ pLD4.