Studies about induction of resistance to the reaction of grafts against their host.
The prevention of reactions of transplanter grafts against their host (GVHR)--the most serious obstacle to transplantations of allogenic bone marrow--still remains an actual problem. The results of studies about the conditions leading to the development of and about the basis of the resistance of /B10 X A/F1 hybrid mice to the GVHR elicited by A/Ph parental spleen cells, having been induced by a previous local or systemic application of various types of parental cells, are presented in this paper. The injection of an adequate dose of A/Ph mouse thymocytes into the paws of /B10 X A/F1 hybrids leads to the development of resistance against within three days, while a similar injection of B10 thymocytes remains without effect. Systemic administration of the A/Ph thymocytes and of cells of the spleen (and also of their fractions differing from one another by their adherence to nylon cotton-wool) induces resistance towards the regional type of the GVHR of A/Ph cells only after as long as seven days; the B10 splenic cells are also effective in this manner. According to this, it appears that the regional type of resistance manifests signs of specificity while the general type of resistance is nonspecific, and also that the degree of resistance is not related to the intensity of the primary GVHR. When the nature of the resistance was analyzed it appeared that both the nylon nonadherent and the adherent fractions of spleen cells of resistant individuals were capable of transferring the inhibition upon lymphocyte of normal individuals. It was, in both cases, the T-lymphocytes which were responsible for the inhibition, as has been demonstrated by the anihilation of this inhibitory activity of the nylon wool adherent cells by a monoclonal anti Thy 1.2 antibody with a complement.
D000818:Animals; D016026:Bone Marrow Transplantation; D003165:Complement System Proteins / Q000276:immunology; D006087:Graft vs Host Reaction; D007113:Immunity, Innate; D007518:Isoantibodies / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D007518:Isoantibodies; C035678:anti-Thy antibody; D003165:Complement System Proteins