A new inhibitor of the long-chain fatty acid transfer across the mitochondrial membrane: 2-(3-methylcinnamylhydrazono)-propionate (BM 42.304).
Using two different assay systems to distinguish between overt and inner forms of carnitine palmitoyl transferase (CPT, EC 2.3.1.21) of intact guinea-pig liver mitochondria, we have shown that the hypoglycemic agent 2-(3-methylcinnamylhydrazono)-propionate (BM 42.304) inhibits the activity of carnitine-acylcarnitine translocase of liver mitochondria. The results offer an explanation for the inhibitory effect of the compound on ketogenesis with oleate but not with octanoate in the perfused guinea-pig liver, previously reported by us (Biochem. Pharmacol. 32, 3405-3412, 1983).
D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002331:Carnitine / Q000378:metabolism; D002333:Carnitine Acyltransferases; D002334:Carnitine O-Palmitoyltransferase / Q000037:antagonists & inhibitors*; D002934:Cinnamates / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D006168:Guinea Pigs; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008316:Malonyl Coenzyme A / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010172:Palmitoylcarnitine / Q000378:metabolism; D014166:Transferases / Q000037:antagonists & inhibitors*
D002934:Cinnamates; D004852:Epoxy Compounds; D005227:Fatty Acids; D007004:Hypoglycemic Agents; D010172:Palmitoylcarnitine; D008316:Malonyl Coenzyme A; C039804:BM 42304; D014166:Transferases; D000217:Acyltransferases; D002333:Carnitine Acyltransferases; D002334:Carnitine O-Palmitoyltransferase; C021756:2-tetradecylglycidic acid; D002331:Carnitine