[Use of semithin sections for the histochemical study of carbohydrate-containing biopolymers in cells and tissues with the aid of lectins].
A sensitive method of histochemical analysis of cellular and tissue glycoconjugates on semithin sections using lectins is suggested. For fixation tissue bioptates were incubated for 4 h in a 2.5% glutaraldehyde in phosphate buffered saline (PBS) at 4 degrees C, then washed for 1 h in 0.2 M glycine in PBS. After epon-araldite embedment and preparation of semithin sections, the resin was removed in saturated ethanol-KOH solution during 5-10 s. Endogenous perooxidase was inactivated in methanol containing 0.3% H2O2. For identification of lectin-binding sites semithin sections were incubated for 30 min in a 0.005% solution of lectin-peroxidase conjugate in PBS and visualized by 0.05% diaminobezidine solution in PBS, containing 0.015% H2O2. The method described ensures good preservation of cellular and tissue glycoconjugates and is highly specific and sensitive.
D000818:Animals; D001704:Biopolymers; D001808:Blood Vessels / Q000378:metabolism; D050260:Carbohydrate Metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006651:Histocytochemistry; D006652:Histological Techniques; D007668:Kidney / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D051381:Rats; D013737:Testis / Q000378:metabolism
D001704:Biopolymers; D037102:Lectins
Primenenie polutonkikh srezov dlia gistokhimicheskogo issledovaniia uglevodsoderzhashchikh biopolimerov kletok i tkaneĭ s pomoshch'iu lektinov.