Expression of c-fos oncogene during hepatocarcinogenesis, liver regeneration and in synchronized HTC cells.
We have studied the expression of c-fos gene in rat hepatoma induced by DENA. An increase of c-fos mRNA concentration was observed after 8 days, but the maximal 5- to 6-fold increase was observed after 70 weeks. This increase was found in perinodular hepatocytes as well as in cancer nodules. c-fos expression was also enhanced during liver regeneration at a period corresponding to cell proliferation. In HTC cells the arrest of the cell cycle at early G1 phase by addition of sodium butyrate was accompanied by a strong increase of c-fos gene expression. However the c-fos mRNA rapidly decreased after removal of sodium butyrate during the progression of the cells in the cell cycle and increased transiently when the cells entered again in G1 phase.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002453:Cell Cycle / Q000187:drug effects; D003584:Cytological Techniques; D005260:Female; D005786:Gene Expression Regulation; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D008115:Liver Regeneration; D009857:Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D002087:Butyrates; D012333:RNA, Messenger; D020148:Butyric Acid