Identification of a trans-dominant mutation affecting proline dehydrogenase in Escherichia coli.
L-Proline dehydrogenase catalyzes the oxidation of L-proline to delta 1-pyrroline-5-carboxylate, a reaction that is an important step in the utilization of proline as a carbon or nitrogen source by bacteria. A mutant of Escherichia coli K-12 lacking L-leucyl-tRNA:protein transferase had been found previously to contain about five times as much proline dehydrogenase activity as its parent strain. This difference has now been shown to be due to the presence in the parent strain of a previously unrecognized mutation. This mutation, which has been designated put-4977, specifically affects proline dehydrogenase rather than proline uptake. Although proline dehydrogenase remains inducible by L-proline in strains carrying the mutation, there is a premature cessation of differential synthesis during induction that results in a lower specific activity. The mutation shows about 50% P1-mediated cotransduction with pyrC and is therefore located at about 22 min on the E. coli chromosome. Merodiploids containing a normal F' factor still exhibit decreased enzyme activity, indicating that the put-4977 mutation is trans-dominant. The mutation cannot be detected in present stocks of the transferase-deficient mutant, suggesting that this mutant is a revertant for put-4977.
D000217:Acyltransferases / Q000378:metabolism; D019881:Aminoacyltransferases; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005799:Genes, Dominant; D005809:Genes, Regulator; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000096:biosynthesis*; D010641:Phenotype; D010957:Plasmids; D011392:Proline / Q000378:metabolism; D011394:Proline Oxidase / Q000096:biosynthesis*
D011392:Proline; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011394:Proline Oxidase; D000217:Acyltransferases; D019881:Aminoacyltransferases; C021511:leucyltransferase; D009584:Nitrogen