Use of chloroquine-treated granulocytes and platelets in the diagnosis of immune cytopenias.
A chloroquine modification of the fluorescent antiglobulin technique has been used to demonstrate cell-specific antibodies in the presence of HLA antibodies. This is of particular value in the diagnosis of alloimmune neonatal thrombocytopenia and neutropenia, post-transfusion purpura, and in the investigation of febrile non-haemolytic transfusion reactions and of patients refractory to platelet transfusions.
D000328:Adult; D000906:Antibodies / Q000032:analysis*; D001323:Autoantibodies; D001327:Autoimmune Diseases / Q000209:etiology; D001792:Blood Platelets / Q000276:immunology; D002738:Chloroquine; D005260:Female; D005334:Fever / Q000209:etiology; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology / Q000637:transplantation*; D006801:Humans; D007231:Infant, Newborn; D008875:Middle Aged; D017713:Platelet Transfusion; D011247:Pregnancy; D013921:Thrombocytopenia / Q000209:etiology* / Q000276:immunology; D065227:Transfusion Reaction
D000906:Antibodies; D001323:Autoantibodies; D002738:Chloroquine