Evaluation of the RIM system and Gono Gen test for identification of Neisseria gonorrhoeae from clinical specimens.
RIM-N (Austin Biological Laboratories) and Gono Gen (Micro Media Systems) were evaluated for accuracy and compared with conventional carbohydrate degradation tests for identification of Neisseria gonorrhoeae. A total of 127 fresh clinical isolates of N. gonorrhoeae were tested; 118 (93%) were identified by RIM-N, and 100 (79%) yielded positive reactions with Gono Gen. Seventy nongonococcal isolates including other Neisseria species, Branhamella catarrhalis, Kingella kingae, and Moraxella species were evaluated to determine the specificity of the test methods. Both rapid tests were 100% specific in the identification of N. gonorrhoeae isolates. RIM-N was the most sensitive test for the identification of N. gonorrhoeae and offers a useful, more rapid alternative to conventional carbohydrate degradation methods.
D000372:Agglutination Tests; D001431:Bacteriological Techniques; D050260:Carbohydrate Metabolism; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification* / Q000378:metabolism; D009346:Neisseriaceae