The actions of leukotrienes C4 and D4 in the porcine renal vascular bed.
The kidney of anaesthetised pigs was perfused in situ with carotid arterial blood. Renal blood flow and perfusion pressure were recorded. Close intra-arterial injection of leukotriene (LT) C4, D4 or noradrenaline (NA) caused a dose-related increase in vascular resistance. Both LTs were more active than NA by one to two orders of magnitude. Systemically-administered indomethacin potentiated the effect of all three agonists. Incubation of renal artery tissue with calcium ionophore A23187 in the presence of indomethacin resulted in the generation of LT-like material which, when assayed on guinea-pig ileum, was indistinguishable from LTD4. The results show that pig renal vessels produce LT-like material and suggest that the potent vasoconstriction induced by exogenous NA and LTs is modulated in vivo by a vasodilator cyclo-oxygenase product.
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D004357:Drug Synergism; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D012079:Renal Circulation / Q000187:drug effects*; D013189:SRS-A / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013552:Swine; D014655:Vascular Resistance / Q000187:drug effects
D013189:SRS-A; D000001:Calcimycin; D009638:Norepinephrine; D007213:Indomethacin