The production of monoclonal antibodies against respiratory syncytial virus and its clinical applications.
The availability of monoclonal antibodies against various viral components, either internal nucleoproteins or membrane proteins, has greatly advanced our understanding of the biochemistry of respiratory syncytial virus (RSV). The components responsible for serum neutralization have been identified by monoclonal antibodies, thus facilitating the development of synthetic RSV vaccine. A number of clinical tests have been devised using monoclonal antibodies for either qualitative or quantitative determination of the presence of RSV antigens. The practical procedures for the widely used indirect immunofluorescent antibody (IFA) and enzyme-linked immunosorbent assays (ELISA) are presented. Various commercial ELISA spectrophotometers and washers are also compared and evaluated.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D002459:Cell Fusion; D002469:Cell Separation; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D005455:Fluorescent Antibody Technique; D005615:Freezing; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007106:Immune Sera; D051379:Mice; D009305:Nasopharynx / Q000382:microbiology; D011309:Preservation, Biological; D011817:Rabbits; D051381:Rats; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D010253:Respirovirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy; D013053:Spectrophotometry / Q000295:instrumentation; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D014764:Viral Proteins; D014765:Viral Vaccines