Active immunization of NMRI mice against Serratia marcescens.
Phenol-hot water lipopolysaccharide (LPS) extracts of Serratia marcescens strains CDC O3:H1, CDC O6:H3, NEW CDC O14:H12, and SH 186 (serotype O6/O14:H12) significantly protected NMRI mice against intraperitoneal challenge with the more mouse virulent homologous strains; overall, there was moderate cross-protection against the minority of heterologous challenge strains. Trichloroacetic acid LPS extracts and K-antigen extracts of strains NEW CDC O14:H12 and SH 186 also proved protective antigens. The purified metalloproteases of strains SH 186 and SF 178 (serotype O6/O14:H12) effected active murine immunization. Neither active nor passive immunization of NMRI mice with E. coli Rc mutant J5 afforded significant protection against various challenge strains of S. marcescens.
D000818:Animals; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000276:immunology; D001424:Bacterial Infections / Q000517:prevention & control*; D010450:Endopeptidases / Q000276:immunology; D005260:Female; D007114:Immunization; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008666:Metalloendopeptidases; D051379:Mice; D012706:Serratia marcescens / Q000276:immunology*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; C030659:K antigens; D008070:Lipopolysaccharides; D010450:Endopeptidases; D008666:Metalloendopeptidases