Comparison of an enzyme-linked immunosorbent assay using crude S. haematobium soluble egg antigen with a radioimmunoassay using purified S. mansoni egg antigen for the serodiagnosis of schistosomiasis haematobium.
An enzyme-linked immunosorbent assay (ELISA) using crude Schistosoma haematobium soluble egg antigen (ShSEA) was compared with a radioimmunoassay (RIA) employing purified heterologous species S. mansoni egg antigen (MSA1) for the serodiagnosis of schistosomiasis haematobium in a group of 45 Nigerian school children living in an area endemic for S. haematobium. Both assay systems appeared applicable. The ELISA proved to be more sensitive detecting 100% of the 27 parasitologically positive individuals while the RIA defined 89% of this group. Neither test had false-positive results for the 10 non-endemic area parasitologically negative controls, although the ELISA demonstrated that 56% of the 18 endemic area parasitologically negative controls had anti-ShSEA antibodies. The RIA indicated that 44% of this group had anti-MSA1 antibodies. These latter findings were interpreted as related to the hyperendemicity of schistosomiasis haematobium for the study area.
D000293:Adolescent; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D010063:Ovum / Q000276:immunology*; D011863:Radioimmunoassay; D012548:Schistosoma haematobium / Q000276:immunology*; D012552:Schistosomiasis / Q000175:diagnosis
D000947:Antigens, Helminth