Establishment of the antiviral state in alpha, beta-interferon-resistant Friend cells treated with gamma-interferon. Induction of 67-kilodalton protein kinase activity in absence of detectable 2-5A synthetase.
Treatment with murine gamma-interferon (IFN) preparations of variant sublines of Friend leukemia cells resistant to the alpha, beta IFN-induced antiviral state (Affabris, E., Jemma, C., and Rossi, G.B. (1982) Virology 120, 441-452; Affabris, E., Romeo, G., Belardelli, F., Jemma, C., Mechti, N., Gresser, I., and Rossi, G. B. (1983) Virology 125, 508-512) results in the establishment of a bona fide antiviral state. In fact, gamma IFN preparations are able to induce a dose-dependent reduction of endogenous virus release and of vesicular stomatitis or encephalomyocarditis viruses yields (up to 1.5 log). Under these experimental conditions, no inducible 2-5A synthetase activity is detectable in cell extracts. The 67-kDa protein kinase, uninducible by treatment with alpha, beta IFN (up to 13,000 units/ml), is instead induced upon treatment with gamma IFN at a similar rate of activity as in wild-type Friend leukemia cells, both when assayed in solution and after immobilization on poly(rI) X poly(rC)-agarose.
D015088:2',5'-Oligoadenylate Synthetase / Q000032:analysis*; D000818:Animals; D004351:Drug Resistance; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D007371:Interferon-gamma / Q000627:therapeutic use*; D007942:Leukemia, Experimental / Q000188:drug therapy* / Q000201:enzymology; D051379:Mice; D011494:Protein Kinases / Q000096:biosynthesis*; D013997:Time Factors
D007371:Interferon-gamma; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase