Immuno gold staining (IGS) for electron microscopical demonstration of glial fibrillary acidic (GFA) protein in LR white embedded tissue.
Post-embedding immunocytochemical staining methods using gold have so far failed to label intermediate filament antigens in situ in epon or araldite embedded tissue. We have now applied the post-embedding immuno gold staining (IGS) technique for LR White embedded tissue. Glial fibrillary acidic (GFA) protein immunoreactivity was clearly demonstrated electron microscopically on astrocytic filaments of rat cerebellum in situ.
D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism*; D006046:Gold; D007382:Intermediate Filaments / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D051381:Rats; D012116:Resins, Plant
D005904:Glial Fibrillary Acidic Protein; D012116:Resins, Plant; D006046:Gold