The purification of urease from Aspergillus nidulans.
A purification procedure is described for Aspergillus urease, the most important step being affinity chromatography on hydroxyurea Sepharose. The enzyme exists as a single active species of Mr 240,000. The pure enzyme has an activity of 670 mumol urea hydrolysed/min, has a Km of 10(-3) M, an optimum pH of 8.5 and a sub-unit Mr of 40,000.
D000645:Ammonium Sulfate / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000201:enzymology*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D014510:Urease / Q000302:isolation & purification*
D014510:Urease; D000645:Ammonium Sulfate