The effect of harmine on the localization of the nucleolar proteins C23 and B23.
In this study we show that harmine treatment (10 mg/l for 2 or 24 h) of PtK2 cells had a marked effect on the localization of the nucleolar phosphoproteins C23 and B23. C23 was localized with silver staining in the fibrillar areas of completely segregated nucleoli. B23 was localized mainly on the periphery of the nucleoli with the aid of immunofluorescence.
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000187:drug effects / Q000648:ultrastructure*; D004173:Dipodomys; D005455:Fluorescent Antibody Technique; D006247:Harmine / Q000494:pharmacology*; D008854:Microscopy, Electron; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D012261:Ribonucleoproteins / Q000032:analysis*
D000470:Alkaloids; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D000090243:Nucleophosmin; D006247:Harmine