A new sensitive and fast peptide immunoassay based on enzyme amplification used in the determination of CGRP and the demonstration of its presence in the thyroid.
An enzyme amplified immunoassay for rCGRP based on cofactor cycling has been found to be clearly superior to a comparable radioimmunoassay employing the same antiserum in terms of sensitivity, speed and convenience. Correlation between the two methods was very good. With the enzyme amplified immunoassay we have been able to demonstrate the existence of rCGRP in thyroid extract.
D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D002851:Chromatography, High Pressure Liquid; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008832:Microchemistry; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013961:Thyroid Gland / Q000032:analysis*
D007106:Immune Sera; D009419:Nerve Tissue Proteins; D015740:Calcitonin Gene-Related Peptide