Identification of proline carrier in Escherichia coli K-12.
Proline carrier, a product of the putP gene of Escherichia coli, was identified as a 35 kDa cytoplasmic membrane protein by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Its identification was based on the following evidence: First, the density of the band corresponding to a 35 kDa protein correlated with the proline-binding activity of cytoplasmic membranes from putP-deficient and putP-amplified strains. Second, by the differential labeling method, the 35 kDa protein was specifically labeled with radioactive N-ethyl-maleimide. The 35 kDa protein was found to aggregate on heat treatment and to show abnormal mobility on SDS-PAGE.
D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D029968:Escherichia coli Proteins; D005033:Ethylmaleimide / Q000378:metabolism; D046911:Macromolecular Substances; D008970:Molecular Weight; D011392:Proline / Q000378:metabolism; D027981:Symporters
D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D027981:Symporters; C041832:proline transport protein; C060609:PutP protein, E coli; D011392:Proline; D005033:Ethylmaleimide