Mapping of functional domains in adenovirus E1A proteins.
We have modified the E1A gene of human subgroup C adenovirus by introducing deletions in its coding sequence. Various truncated E1A proteins were expressed in Escherichia coli, purified, and microinjected via glass capillaries into Vero cells. We monitored their movement from the cell cytoplasm to the nucleus and their ability to induce expression of H5dl312, an adenovirus E1A deletion mutant. Our results show that the carboxyl terminus of E1A contains sequences essential for rapid and efficient nuclear localization. Essential information for efficient H5dl312 complementation is contained in an internal region, comprising sequences of both exons of the E1A gene. A first exon-encoded region, however, is sufficient to induce low levels of adenovirus gene expression. Information for nuclear localization and for H5dl312 complementation are therefore encoded by distinct domains of the E1A gene. In addition, we determined that the human c-myc product was unable to complement H5dl312.
D000256:Adenoviridae / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D009154:Mutation; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D014764:Viral Proteins