[Replication of Streptomyces plasmids: the DNA nucleotide sequence of plasmid pSB 24.2].
The nucleotide sequence of DNA in plasmid pSB 24.2, a natural deletion derivative of plasmid pSB 24.1 isolated from S. cyanogenus was studied. The plasmid amounted by its size to 3706 nucleotide pairs. The G-C composition was equal to 73 per cent. The analysis of the DNA structure in plasmid pSB 24.2 revealed the protein-encoding sequence of DNA, the continuity of which was significant for replication of the plasmid containing more than 1300 nucleotide pairs. The analysis also revealed two A-T-rich areas of DNA, the G-C composition of which was less than 55 per cent and a DNA area with a branched pin structure. The results may be of value in investigation of plasmid replication in actinomycetes and experimental cloning of DNA with this plasmid as a vector.
D001482:Base Composition; D001483:Base Sequence; D002874:Chromosome Mapping; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D005821:Genetic Techniques; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial
Izuchenie replikatsii plazmid Streptomyces: nukleotidnaia posledovatel'nost' DNK plazmidy pSB 24.2.