Induction of interleukin 2 responsiveness in thymocytes by synergistic action of interleukin 1 and interleukin 2.
Thymocyte cultures from C3H/HeJ mice were stimulated for proliferative responses with purified preparations of interleukin 1 (IL 1) and interleukin 2 (IL 2). Synergistic responses were obtained in the absence of mitogen. In the presence of excess IL 2, the thymocyte proliferation response was strictly dependent on the amount of IL 1 in the cultures. Antibodies to IL 1 inhibited the response in a dose-dependent manner. The combination of IL 1 plus IL 2 induced the appearance of IL 2 receptors on murine thymocytes as detected with a monoclonal antibody directed against the IL 2 receptor. Neither IL 1 nor IL 2 alone had this effect. The thymic subpopulation found to become IL 2 responsive upon IL 1 stimulus was the peanut agglutinin-negative (PNA-) medullary fraction.
D000818:Animals; D004357:Drug Synergism; D007108:Immune Tolerance; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology*; D007376:Interleukin-2 / Q000378:metabolism / Q000502:physiology*; D037102:Lectins; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D019887:Peanut Agglutinin; D010641:Phenotype; D011971:Receptors, Immunologic / Q000096:biosynthesis; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000378:metabolism
D007375:Interleukin-1; D007376:Interleukin-2; D037102:Lectins; D019887:Peanut Agglutinin; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2