Immunodissection of sperm surface modifications during epididymal maturation.
Mammalian spermatozoa undergo changes in morphology, composition, and function during transit through the epididymis. These changes correlate with acquisition by sperm of the ability to fertilize ova. It has been found that sperm from the cauda epididymidis, but not those from the caput epididymidis, are able to bind to the zona pellucida. This would imply a modification in sperm surface characteristics. Biochemical and immunological studies have demonstrated changes in sperm surface composition during epididymal maturation. These changes involve addition of epididymal secretory products to the sperm surface, loss or alteration of existing sperm surface molecules, and possibly the unmasking of preexisting molecules or epitopes. Several laboratories have studied the epididymal secretory proteins in the rat, but a consensus has not been reached on the identification, characterization, source, and sperm surface association of these proteins. Monoclonal antibodies are beginning to be used to characterize sperm surface components and sperm maturation antigens. They are proving to be valuable tools for the dissection of epididymal maturation when used in conjunction with biochemical and physiological approaches.
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D002462:Cell Membrane / Q000502:physiology; D004210:Dissection; D004822:Epididymis / Q000378:metabolism / Q000502:physiology*; D007106:Immune Sera / Q000276:immunology; D007158:Immunologic Techniques; D008297:Male; D008322:Mammals / Q000502:physiology*; D051379:Mice; D013079:Sperm Maturation; D013094:Spermatozoa / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D007106:Immune Sera