Xeno- and auto-perfusion of rabbit kidney. Machine perfusion with blood at 37 degrees C.
Five rabbit kidneys were perfused with human blood and another five with their own blood in a re-circulating oxygenated system at 37 degrees C. The flow decreased to 2 ml/min. within 30 min. in all xenoperfusions, while none of the autoperfused had decreased to this level by 60 min. Endothelial damage, exudation, and IgG deposits along the basement membrane of the glomerular capillaries were the discriminative features of the xenoperfusion. In these experiments, we were unable to demonstrate any major role of platelets in the process leading to decreased blood flow.
D000818:Animals; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007668:Kidney / Q000276:immunology / Q000473:pathology; D016030:Kidney Transplantation; D010477:Perfusion / Q000379:methods*; D010974:Platelet Aggregation; D011817:Rabbits; D012079:Renal Circulation; D014183:Transplantation, Heterologous
D007074:Immunoglobulin G