Influence of salts and temperature on the transfer of mercury resistance from a marine pseudomonad to Escherichia coli.
Thirty-one strains of marine bacteria were examined for their ability to transfer mercury resistance to Escherichia coli in complex media; eight strains were able to transfer their resistance marker, with frequencies ranging from 10(-3) to 10(-8). Frequencies generally increased with the increase of the mating period. Additional mating experiments were carried out with one strain, belonging to the pseudomonads, to estimate the influence of temperature, salinity, and time on the conjugal transfer frequency of mercury resistance markers. The higher frequencies occurred at 30 degrees C, in a salt medium (37%), after 24 h of mating.
D003227:Conjugation, Genetic; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D005798:Genes, Bacterial; D005819:Genetic Markers; D008628:Mercury / Q000494:pharmacology*; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D011815:R Factors; D012623:Seawater; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
D004269:DNA, Bacterial; D005819:Genetic Markers; D012965:Sodium Chloride; D008628:Mercury