Immunological quantitation of tyrosinase from wild-type and albino mutant mice.
The relationship between gene dosage, enzyme activity, and level of immunologically cross-reacting material (CRM) was examined in mammalian tyrosinase (EC 1.14.18.1) by rocket immunoelectrophoresis. Skin extracts from mice heterozygous (C/c) and homozygous (c/c) for the albino locus contain 46% and 0% of CRM, respectively, as compared with wild-type (C/C) animals. Enzyme activity and CRM level were directly proportional in these genotypes, suggesting that the albino locus controls the quantity of tyrosinase produced in melanocytes.
D000818:Animals; D004156:Catechol Oxidase / Q000032:analysis*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D051379:Mice; D008817:Mice, Mutant Strains / Q000378:metabolism*; D008970:Molecular Weight; D014442:Monophenol Monooxygenase / Q000032:analysis*; D012867:Skin / Q000201:enzymology
D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase