Ultrastructural identification of VIP-containing nerve fibres in the myenteric plexus of the rat ileum.
Vasoactive intestinal polypeptide (VIP)-containing nerve fibres of myenteric plexus of the rat ileum were investigated using the pre-embedding peroxidase-antiperoxidase technique for electron microscopy. The VIP-positive fibres were characterized by a predominance of spherical agranular vesicles with a diameter of 40-55 nm. The immunoprecipitate was largely localized on the vesicle membrane. Large granular vesicles (100-144 mm) containing labelled dense cores were also present within labelled nerve profiles. Quantitative data indicate that the vesicles appeared in the following proportions: 93% small agranular vesicles and 7% large granular vesicles.
D000818:Animals; D007082:Ileum / Q000294:innervation*; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D009197:Myenteric Plexus / Q000032:analysis / Q000648:ultrastructure*; D009412:Nerve Fibers / Q000032:analysis / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013572:Synaptic Vesicles / Q000032:analysis / Q000648:ultrastructure; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D014660:Vasoactive Intestinal Peptide