beta 2-Microglobulin from serum associates with several class I antigens expressed on the surface of mouse L-cells.
Bovine beta 2-microglobulin (beta 2-m) present in fetal calf serum (FCS) is able to replace endogenous beta 2-m associated with several class I antigens from human and mouse cells maintained in culture [Bernabeu et al. (1984) Nature, Lond. 308, 642-645]. Here we show that human HLA-A2 and HLA-B7, as well as mouse H-2Ld and H-2Dd heavy chains expressed after gene transfer in mouse L-cells, associate to a large extent with bovine beta 2-m. We also demonstrate that bovine beta 2-m associated with the endogenous H-2Kk/Dk heavy chains generates an antibody response when L-cells are injected into syngeneic C3H mice.
D000818:Animals; D005312:Fetal Blood / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology*; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D064532:Histocompatibility Antigen H-2D; D007739:L Cells / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D014162:Transfection; D001613:beta 2-Microglobulin / Q000032:analysis / Q000276:immunology*
D006183:H-2 Antigens; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D064532:Histocompatibility Antigen H-2D; D001613:beta 2-Microglobulin