Detection of human acid alpha-glucosidase in fibroblasts using monoclonal antibodies in a biotin-avidin amplified ELISA.
A sensitive and specific immunological assay for detection of human lysosomal alpha-glucosidase was developed using a mouse monoclonal antibody incorporated into a biotin-avidin amplified ELISA. The immunoassay was more than 60 times more sensitive than the currently used enzymatic assay for alpha-glucosidase activity using a fluorimetric substrate. This methodology provides an alternative approach with increased sensitivity for screening individuals for alpha-glucosidase deficiency.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001360:Avidin; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005347:Fibroblasts / Q000201:enzymology; D005959:Glucosidases / Q000276:immunology*; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D000520:alpha-Glucosidases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D005959:Glucosidases; D000520:alpha-Glucosidases