Why is processing of 23 S ribosomal RNA in Escherichia coli not obligate for its function?
In an RNase III-deficient mutant of Escherichia coli, all 23 S ribosomal RNA in ribosomes is present in an unprocessed form with a double-stranded stem at the base of the molecule stable enough to be detected by electron microscopy under conditions where all other secondary structure is denatured. Molecules with variable stem lengths enter freely into polysomes, consistent with the existence of a similar but much shorter stem in mature 23 S rRNA in wild-type ribosomes.
D004926:Escherichia coli / Q000235:genetics*; D008854:Microscopy, Electron; D009154:Mutation; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000235:genetics*; D012270:Ribosomes / Q000648:ultrastructure
D012335:RNA, Ribosomal