Stimulation of proliferation in mixed cultures of mouse tumor cells and nonimmune peritoneal cells. II. Stimulation of tumor cells by macrophages and of lymphocytes by a tumor cell product.
Stimulation of [methyl-3H]thymidine incorporation in mixed cultures of mouse peritoneal cells (PC) and tumor cells required a large adherent PC. Flow cytometric analysis, as well as autoradiography, showed that the stimulation involved increased proliferation of tumor cells. The stimulation required contact between tumor and PC; it was partly inhibited by Trasylol (aprotinin) and potentiated by dexamethasone. In addition, there was minor stimulation of PC by a soluble product of tumor cells; this stimulation was not affected by Trasylol or dexamethasone. It is suggested that macrophages can stimulate tumor cell proliferation and that such stimulation may be an important factor in host-tumor relationships.
D000818:Animals; D001202:Ascitic Fluid; D001345:Autoradiography; D002450:Cell Communication; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005434:Flow Cytometry; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D013936:Thymidine / Q000378:metabolism
D008937:Mitomycins; D016685:Mitomycin; D003907:Dexamethasone; D013936:Thymidine