Alterations in follicular fluid steroids and follicular hCG and FSH binding during atresia in hamster.
Preovulatory follicles from hamsters treated on proestrus for 1-3 days with phenobarbital sodium exhibited early signs of atresia after 2-3 days of ovulatory delay. A significant increase in follicular fluid progesterone was evident by Day 1 of delay. Concentrations of androstenedione in follicular fluid were unaffected by ovulatory delay. Follicular fluid levels of estradiol in delayed follicles were either higher than proestrous values after 1 day of delay or lower after 2 and 3 days of delay. hCG binding was slightly higher than proestrous controls after ovulatory delay whereas FSH binding was significantly lower than controls after 2 and 3 days of ovulatory delay. These results indicate that in the barbiturate-treated hamster the elevated follicular fluid levels of progesterone precede by 1-2 days the previously reported increase in steroidogenic capability of delayed follicles to produce progesterone in vitro; this correlated with an increase in the ratio of hCG:FSH binding and this was mostly due to a decrease in FSH binding to whole follicles.
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D006224:Cricetinae; D004958:Estradiol / Q000378:metabolism; D004971:Estrus; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D005496:Follicular Atresia; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D006080:Ovarian Follicle / Q000378:metabolism*; D010060:Ovulation / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology; D011247:Pregnancy; D011359:Proestrus; D011374:Progesterone / Q000378:metabolism; D011863:Radioimmunoassay; D013997:Time Factors
D006063:Chorionic Gonadotropin; D012739:Gonadal Steroid Hormones; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D010634:Phenobarbital