[Biosynthesis of tritium-labeled S-adenosyl-L-methionine in yeast cells].
Biosynthetic preparation of S-adenosyl-L-[methyl-3H]methionine from L-[methyl-3H]methionine by cultivation of diploid yeast Saccharomyces cerevisiae (methionine-auxotrophic) in a cultural medium with the high concentration of L-methionine is described. The radiochemical purity was over 95%. Biological activity of the preparations has been shown in transmethylation reactions in the presence of the yeast homocysteine-methyltransferase.
D007700:Kinetics; D012436:S-Adenosylmethionine / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D012436:S-Adenosylmethionine
Biosintez mechennogo tritiem S-adenozil-L-metionina drozhzhevymi kletkami.