Purification of tissue forms (Amastigotes) of Trypanosoma cruzi by immunoaffinity chromatography.
A rapid and simple method for the purification of amastigotes of Trypanosoma cruzi from spleens of infected mice is described. A protein A-Sepharose 4B immunoadsorbent column bound with antisera to epimastigotes of T. cruzi was used to purify the tissue forms of this parasite. Host cells and debris are not retained, and parasites can be eluted in high yields and purity. Studies of surface glycoproteins and glycolipids of the purified amastigotes with 18 lectins of various specificities revealed the presence on the parasites of receptors for N-acetylglucosamine, N-acetylgalactosamine, D-galactose, and D-mannose binding lectins.
D000371:Agglutination; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D014355:Chagas Disease / Q000469:parasitology; D002846:Chromatography, Affinity; D007163:Immunosorbent Techniques; D037102:Lectins / Q000494:pharmacology; D051379:Mice; D013154:Spleen / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000276:immunology / Q000302:isolation & purification*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D037102:Lectins