[The use of immunoenzyme methods in virology].
Data on the development of theoretical basis of immunoenzyme (IE)method s (EMIT analysis and ELISA) and on using these methods in virology are presented. The problems arising in the use of most popular version of IE analysis. ELISA, are elucidated: the schemes for different variants of ELISA; the demands to adsorbent, enzyme and substrate for achieving the maximum of sensitivity of the technique; comparison of the advantages of different ELISA variants in identification of various antigens and antibodies. The data on using ELISA for indication and identification of viral antigens and virus specific antibodies are analyzed. The respiratory viruses have been used to demonstrate the possibilities of IE technique for rapid diagnostic of viral diseases and for studies in viral reproduction and structure.
D007124:Immunoenzyme Techniques; D014773:Virology
Ispol'zovanie immunofermentnykh metodov v virusologii.