[Isolation and mapping of a mutation leading to damage in the cytoplasmic-specific component of the fructose transport system in Escherichia coli K-12 bacteria].
A mutant delta ptsH of Escherichia coli was used to obtain a mutation which damages a function of cytoplasmic specific component of the fructose phosphotransferase system--FPr protein. This mutation was mapped using a number of genetical methods. In conjugational crosses the mutation was localized at 47 min of the E. coli chromosomal map. The gene responsible for this defect was designated fpr. In P1 mediated transduction and in conjugational three-factorial crosses the order of the markers in this region was established as: gyrA-ompC-fpr-ptsF-...-his. The frequency of cotransduction of the fpr gene was 4.5 and 35% with gyrA and ompC markers, respectively. In fructose containing minimal medium the doubling time of the ptsH+fpr mutant was lower than that of the delta ptsHfpr+ mutant. Also, the doubling time of the fpr mutant depends on concentration of fructose in growth medium but is independent of the amount of this sugar in the case of the delta ptsH mutant.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001692:Biological Transport; D002874:Chromosome Mapping; D003593:Cytoplasm / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005632:Fructose / Q000378:metabolism*; D005798:Genes, Bacterial; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000096:biosynthesis / Q000235:genetics; D010766:Phosphorylation
D001426:Bacterial Proteins; D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr
Vydelenie i kartirovanie mutatsii, privodiashcheĭ k povrezhdeniiu tsitoplazmaticheskogo spetsificheskogo komponenta sistemy transporta fruktozy u bakteriĭ Escherichia coli K-12.