[Trypanosoma cruzi: differences in the requirement for protein protectors presented by 2 Argentinian strains].
The protein protector requirements for CA-I and RA T. cruzi trypomastigotes were standardized. The CA-I trypomastigotes needed a supplement of 5% serum or 5% bovine albumin in the washing and/or resuspending solutions to preserve their viability. For RA trypomastigotes, requirements were in the order of 1%. Trypomastigotes viability was determined by motility at the fresh microscopic observation and by mouse inoculation. This is a further demonstration of the particular behaviour reported for the CA-I bloodstream forms compared with those of RA.
D000818:Animals; D002021:Buffers / Q000494:pharmacology; D002465:Cell Movement; D051379:Mice; D010274:Parasitology / Q000379:methods; D011489:Protein Denaturation; D011506:Proteins; D014349:Trypanosoma cruzi / Q000145:classification* / Q000187:drug effects / Q000254:growth & development
D002021:Buffers; D011506:Proteins
Trypanosoma cruzi: diferencias en el requerimiento de protectores proteicos presentadas por dos cepas argentinas.