Comparison of beta-glucan structures in a cell wall mutant of Saccharomyces cerevisiae and the wild type.
A mutant of Saccharomyces cerevisiae defective in the cell wall beta-glucan structure was obtained. The mutant cells are extremely sensitive to (beta 1-3)-glucanase digestion and mild alkali treatment. Structural analysis revealed that the alkali-insoluble, skeletal glucan from wild type cells contains two components, a (beta 1-3) linked glucan with a laminated structure, and a highly branched glucan containing predominantly (beta 1-6) linkages. The mutant cells lack the latter component.
D002473:Cell Wall / Q000032:analysis; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D005936:Glucans / Q000032:analysis*; D006868:Hydrolysis; D008745:Methylation; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000235:genetics
D005936:Glucans