A simple disk-diffusion test for differentiation of yeast species.
A disk-diffusion method for identification of yeasts was developed that depended on their different but distinct susceptibilities to the following chemicals: janus green, ethidium bromide, 2, 3, 5-triphenyltetrazolium chloride, brilliant green, cycloheximide and rhodamine 6G. For 594 of 623 routinely isolated yeasts, the disk-diffusion and the commercial API 20C auxanogram tests gave the same identification, an agreement of 95.3%. Only 8 of 1052 isolates from clinical specimens were not identified by the disk-diffusion method. The method is simple, inexpensive and technically straightforward and for most isolates gives an identification in 24 h.
D001391:Azo Compounds / Q000494:pharmacology; D002175:Candida / Q000145:classification / Q000187:drug effects; D004396:Coloring Agents / Q000494:pharmacology*; D003454:Cryptococcus / Q000145:classification / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004996:Ethidium / Q000494:pharmacology*; D005848:Geotrichum / Q000145:classification / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D009181:Mycoses / Q000382:microbiology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012235:Rhodamines / Q000494:pharmacology; D012248:Rhodotorula / Q000145:classification / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000187:drug effects; D013045:Species Specificity; D013778:Tetrazolium Salts / Q000494:pharmacology*; D015003:Yeasts / Q000145:classification* / Q000187:drug effects
D001391:Azo Compounds; D004396:Coloring Agents; D000644:Quaternary Ammonium Compounds; D012235:Rhodamines; D013778:Tetrazolium Salts; C026188:rhodamine 6G; C009591:triphenyltetrazolium; D003513:Cycloheximide; C047446:janus green; D004996:Ethidium; C006798:brilliant green