PAF-acether-induced release of tissue-type plasminogen activator from vessel walls.
Platelet-activating factor (PAF-acether; 1-0-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) induced the release of plasminogen activator in rat, both in vivo and in perfused hind legs. The released plasminogen activator was shown by immunologic and functional criteria to be tissue-type plasminogen activator (t-PA). Release of t-PA by PAF-acether could be inhibited by phospholipase inhibitors and by lipoxygenase inhibitors, but not by cyclooxygenase inhibitors. It is suggested that PAF-acether induces the release of t-PA from vascular endothelial cells by the (calcium-dependent) activation of a phospholipase-lipoxygenase pathway.
D000818:Animals; D001808:Blood Vessels / Q000378:metabolism*; D002118:Calcium / Q000502:physiology; D016861:Cyclooxygenase Inhibitors; D005342:Fibrinolysis / Q000187:drug effects; D016859:Lipoxygenase Inhibitors; D008297:Male; D010740:Phospholipases / Q000037:antagonists & inhibitors; D010960:Plasminogen Activators / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D010972:Platelet Activating Factor; D010740:Phospholipases; D010960:Plasminogen Activators; D002118:Calcium