Immunocytochemical demonstration of p21 ras family oncogene product in normal mucosa and in premalignant and malignant tumours of the colorectum.
Study of the distribution of the p21 ras oncogene product as demonstrated by monoclonal antibody Y13-259 shows this protein to be apparently present in all epithelial populations of both premalignant and malignant tumours and throughout the normal foetal and adult epithelial crypt population in the colorectum. Metastatic tumour in liver shows a similar staining pattern which is less intense however than in the surrounding normal hepatocytes. Our results suggest that the presence of this protein is a widespread feature of normal cellular metabolism in certain cell types and is not restricted to those actively involved in cellular proliferation. It appears, furthermore, that neither cells at different stages of carcinogenesis nor those representing variants of a malignant phenotype can be identified using this particular antibody.
D000911:Antibodies, Monoclonal / Q000276:immunology; D003106:Colon / Q000032:analysis; D003110:Colonic Neoplasms / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D009857:Oncogenes; D011230:Precancerous Conditions / Q000032:analysis*; D016283:Proto-Oncogene Proteins p21(ras); D012004:Rectal Neoplasms / Q000032:analysis*
D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)