Molecular species of platelet-activating factor generated by human neutrophils challenged with ionophore A23187.
Two species of platelet-activating factor (PAF), 1-hexadecyl- and 1-octadecyl-2-acetyl-sn-glycero-3-phosphocholine (C16 = 0 AGEPC and C18 = 0 AGEPC) were detected in ionophore A23187-stimulated human neutrophils. The amount of AGEPC in 1 x 10(7) neutrophil cells was 80 +/- 26 pmol (mean +/- standard error) with a range of 14 to 223 pmol (n = 8), and it consisted of 80% of the C16 = 0 species and 20% of the C18 = 0 species. Most of the AGEPC derived from ionophore-treated neutrophils remained cell associated rather than being secreted into the medium, even when the medium contained ample albumin protein, which can trap AGEPC. These results were obtained by a technique of gas chromatography-mass spectrometry coupled with selected ion monitoring.
D000328:Adult; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000097:blood; D010972:Platelet Activating Factor / Q000032:analysis / Q000096:biosynthesis*; D010974:Platelet Aggregation; D011817:Rabbits
D010713:Phosphatidylcholines; D010972:Platelet Activating Factor; D000001:Calcimycin