[Evaluation of an immunoenzymatic method for the simultaneous determination of HBsAg and anti-HBs].
In this study we have evaluated an enzymeimmunoassay for the simultaneous detection of HBsAg and anti-HBs. The EIA method was found to determine anti-HBs at titer of about 10 mIU/ml and HBsAg between 1.5 and 2.5 EU/ml for both ad and ay subtypes. Detection of HBsAg and anti-HBs by EIA and RIA in sera collected from healthy subjects and in patients with acute or chronic hepatitis showed a concordance, respectively, of 98.2 and 97.4%. The discrepancies were in part due to the higher sensitivity of RIA respect to EIA method, while in some cases EIA seems to detect anti-HBs earlier than RIA. The EIA method might be effectively employed in pre-vaccine screening and in the serological follow-up of individuals who received anti-hepatitis B vaccine.
D000328:Adult; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006525:Hepatitis, Viral, Human / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D014761:Viral Hepatitis Vaccines / Q000276:immunology
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D014761:Viral Hepatitis Vaccines
Valutazione di un metodo immunoenzimatico per la determinazione contemporanea dell'HBsAg e dell'anti-HBs.