Nature of the residual alpha-1,4-glucosidase activity in the seminal plasma of vasectomized men.
Vasectomy leads to a drastic decrease of neutral alpha-1,4-glucosidase from human seminal plasma. The nature of this residual enzyme activity has been ascertained according to optimum pH and sucrose density gradient analysis with or without inhibitors of neutral (maltotriose) or acid (sodium dodecyl sulfate) alpha-1,4-glucosidase. Data from the present study provide strong evidence that the enzyme content of the seminal plasma is of mixed nature after exclusion of the epididymis.
D000328:Adult; D003956:Dialysis; D005959:Glucosidases / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D012661:Semen / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014312:Trisaccharides / Q000494:pharmacology; D014461:Ultracentrifugation; D014659:Vasectomy; D000520:alpha-Glucosidases / Q000378:metabolism*
D065089:Glycoside Hydrolase Inhibitors; D014312:Trisaccharides; D012967:Sodium Dodecyl Sulfate; C008317:maltotriose; D005959:Glucosidases; D000520:alpha-Glucosidases