[Properties of the phospholipases C from Bacillus cereus].
Three different phospholipases C--the so-called phospholipase C which hydrolyses phosphatidylcholine (Pch-PLC), sphingomyelinase (SM-PLC) and phosphatidylinositol hydrolysing phospholipase C (PI-PLC)--were separated from the culture filtrate of Bacillus cereus using column chromatography on DEAE-sephadex A-50. The pI values were estimated to be 5.0 +/- 0.3 for PI-PLC and 5.3 +/- 0.2 for SM-PLC. The effect of some bivalent cations was studied. Metal ions had no effect on the activity of PI-PLC, whereas Mg2+ and Co2+ in concentrations from 1 to 5 mM highly activated SM-PLC. Mg2+ failed to activate PCh-PLC, and Co2+ even inhibited it. EDTA of o-phenantroline had a rather inhibitory effect on Pch-PLC, but they almost did not affect SM-PLC.
D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology*; D002384:Catalysis; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004530:Egg Yolk; D005260:Female; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010740:Phospholipases / Q000302:isolation & purification*; D013109:Sphingomyelins / Q000378:metabolism; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D015002:Yeast, Dried
D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D013109:Sphingomyelins; D010740:Phospholipases; D010738:Type C Phospholipases
Nekotorye svoĭstva fosfolipaz C iz Bacillus cereus.