Mechanism of enhancing effect of irradiation on production of IL-2.
In order to explain the mechanism of enhancing effect of irradiation on IL-2 production, assumption that radio-induced free radicals could act as a signal for mitogenesis and/or increase lipid peroxidation of T lymphocyte membrane phospholipids was made. Thus, production of metabolites of the lipoxygenase pathway in the arachidonic acid (AA) cascade, known to increase T cell proliferation and lymphokine production could be enhanced by irradiation. We have shown that (1) hydroxyl radical scavenger (DMSO) abolished IL-2 production, (2) inhibitor of the lipoxygenase pathway (NDGA) strongly decreased IL-2 production, (3) thin layer chromatography demonstrated a polar metabolite(s) of AA in increasing amounts according to the following order: non-irradiated resting cells, non-irradiated stimulated cells, irradiated resting cells, and irradiated stimulated cells. Investigations are in progress to identify this metabolite.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis*; D009637:Masoprocol; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*
D001095:Arachidonic Acids; D002396:Catechols; D005609:Free Radicals; D007376:Interleukin-2; D016718:Arachidonic Acid; D009637:Masoprocol; D004121:Dimethyl Sulfoxide