Deficiency of NK activity of HNK-1+ cells after transplantation of fetal thymus and liver or haploidentical soybean agglutinin-treated marrow cells in two severe combined immunodeficiency patients.
Two severe combined-immunodeficiency patients successfully transplanted with fetal thymus and liver or haploidentical lectin-treated marrow cells lacked NK activity, with a normal number of HNK1+ cell-defined NK cells. The defect was not due to the inhibiting factor in patients' sera. Their NK cells bound to their targets, but did not lyse them in a single-cell agarose assay, and did not respond to alpha-IFN or IL-2. IL-2 did not stimulated the development of mature NK cells that bear M1 antigens from precursors that lack M1 antigens.
D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003602:Cytotoxicity, Immunologic; D006238:Haploidy; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000276:immunology* / Q000628:therapy; D007223:Infant; D007370:Interferon Type I / Q000494:pharmacology; D007376:Interleukin-2 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D037102:Lectins / Q000494:pharmacology; D008099:Liver / Q000166:cytology; D016031:Liver Transplantation; D008297:Male; D037121:Plant Lectins; D030262:Soybean Proteins; D013950:Thymus Gland / Q000166:cytology / Q000637:transplantation*; D010835:Phytohemagglutinins
D007370:Interferon Type I; D007376:Interleukin-2; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins