Comparison of measurements of serum venom-specific IgG by radioimmunoassay and enzyme immunoassay.
Honeybee venom (BV)-, yellow jacket venom (YJV)- and honeybee venom phospholipase A (PLA)-specific IgG were measured by radioimmunoassay (RIA) and enzyme immunoassay (RIA) in 54 sera of insect-allergic patients. In the majority of sera, results of the two tests were similar. Discordant results were found in 1 of 21 sera in tests with PLA, 4 of 23 sera in tests with BV, and 1 of 16 sera in tests with YJV. The RIA measured higher antibody titers in 5 of these 'outlier' sera. The results suggest that with a few exceptions, the two assays are similar for detection of venom- and PLA-specific IgG.
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D001180:Arthropod Venoms / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D011863:Radioimmunoassay; D014688:Venoms / Q000276:immunology*
D000906:Antibodies; D001180:Arthropod Venoms; D007074:Immunoglobulin G; D014688:Venoms