Effect of membrane fluidity upon binding of Electrophorus acetylcholinesterase to lipid vesicles.
A previous report (Watkins, M.S., Hitt, A.S. and Bulger, J.E. (1977) Biochem. Biophys. Res. Commun. 79, 640-647) has indicated that the asymmetric forms of Electrophorus acetylcholinesterase bind exclusively to sphingomyelin vesicles through interaction with the collagen-like 'tail' portion of the enzyme. We report here that acetylcholinesterase also binds to phosphatidylcholine vesicles containing saturated fatty acyl chains and to egg phosphatidylcholine vesicles containing cholesterol. This suggests preferential binding of acetylcholinesterase to membranes of lower fluidity. Surface charge of vesicles and density of zwitterionic lipid headgroups do not significantly affect binding of native acetylcholinesterase. The presence of chondroitin sulfate or hyaluronic acid slightly increases the binding of native acetylcholinesterase to sphingomyelin vesicles, while the presence of 1 M NaCl, bovine serum albumin, or tissue fractions enriched in basement membrane diminish binding. The dissociation constant for native acetylcholinesterase and sphingomyelin vesicles is (1.0-1.5) X 10(-7) M, as measured by a flotation binding assay. The globular, 11S form of acetylcholinesterase also binds to lipid vesicles, although not to the same degree as native acetylcholinesterase. This suggests that the collagen tail of the enzyme enhances binding, but is not essential for binding to occur. These results are consistent with the location of acetylcholinesterase on the surface of the postsynaptic plasma membrane in vivo.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002809:Chondroitin Sulfates / Q000378:metabolism; D004557:Electric Organ / Q000201:enzymology; D004593:Electrophorus; D007700:Kinetics; D008051:Lipid Bilayers / Q000378:metabolism*; D008560:Membrane Fluidity; D013109:Sphingomyelins / Q000378:metabolism
D008051:Lipid Bilayers; D013109:Sphingomyelins; D002809:Chondroitin Sulfates; D000110:Acetylcholinesterase