Suboptimal C3b/C3bi deposition and defective yeast opsonization. II. Partial purification and preliminary characterization of an opsonic co-factor able to correct sera with the defect.
Using a correction assay a factor essential for normal deposition of C3 fragments on zymosan was identified in fractions of serum obtained by Sephacryl S-300 gel filtration (eluting between IgG and albumin), preparative Pevikon block electrophoresis (eluting in the beta-region) and DEAE-ion-exchange chromatography (eluting immediately post IgG). Active material was also identified in a commercial preparation of transferrin. A combination of DEAE-ion-exchange chromatography and gel filtration was used to partially purify the co-factor. The factor has an approximate relative molecular mass of 70,000-80,000.
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003179:Complement C3b / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D009895:Opsonin Proteins / Q000276:immunology*; D011414:Properdin / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000276:immunology; D014168:Transferrin / Q000032:analysis; D015054:Zymosan
D009895:Opsonin Proteins; D014168:Transferrin; D011414:Properdin; D003179:Complement C3b; D015054:Zymosan