Monoclonal antibodies against MHC class II antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line.
A series of seven monoclonal antibodies recognizing cell surface antigens of similar distribution on a panel of leukemia/lymphoma and lymphoblastoid human cell lines was prepared by hybridoma technique after immunization with non-T, non-B acute lymphoblastic leukemia cell line REH. Immune reactivity of these monoclonal antibodies with B-lymphoblastoid and lymphoma cell lines, as well as with non-T, non-B leukemia cell lines but not with T-leukemic and myeloid leukemic cell lines (with the exception of myeloid leukemia cell line KG 1) was demonstrated by indirect immunofluorescence. No reactivity was observed with the examined human non-hemopoietic tumor cell lines, except melanoma cell lines HMB-2 and SK 1477. These antibodies immunoprecipitated a similar cell surface bimolecular glycoprotein complex consisting of two glycosylated chains (gp30, 35), as demonstrated by immunoprecipitation of cell surface 125I-lactoperoxidase radioiodinated, periodate/tritiated borohydride radiolabeled and 35S-methionine metabolically radiolabeled proteins of REH cells. These properties, typical of MHC class II antigens correspond also to immunoperoxidase staining of lymph nodes with some selected antibodies.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008285:Major Histocompatibility Complex; D008565:Membrane Proteins / Q000276:immunology; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D008565:Membrane Proteins