Effects of metabolic inhibitors on the distribution of 45-Ca in subcellular fractions from pancreatic islets.
It is well established that intracellular calcium buffering is of vital importance for the regulation of insulin release. The metabolic inhibitors, cyanide and N-ethylmaleimide, both decreased the 45-Ca content of a mitochondrial fraction isolated from glucose-stimulated pancreatic islets. We suggest that the main mechanism behind this decrease is an impaired uptake of the isotope, since cyanide had no effect on the 45-Ca wash-out from the mitochondrial fraction of preloaded islets.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology*; D005033:Ethylmaleimide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D011190:Potassium Cyanide / Q000494:pharmacology*; D051381:Rats; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D003486:Cyanides; D011190:Potassium Cyanide; D005033:Ethylmaleimide; D002118:Calcium