Low temperature embedding with Lowicryl resins: two new formulations and some applications.
Lowicryl K4M and HM20 are methacrylate/acrylate based low temperature embedding resins for biological material which can be used in conjunction with either the progressive lowering of temperature (PLT) technique or with freeze-substitution. K4M and HM20 are applicable over a very extended temperature range, approximately 220 K to 340 K. With two new resins, K11M and HM23, one can reach even lower temperatures, c. 200 K. Freeze-substitution combined with low temperature embedding allows for very mild or no chemical fixation which seems to increase the sensitivity of immunocytochemical localization of antigens on sections.
D000180:Acrylic Resins; D000818:Animals; D004926:Escherichia coli / Q000032:analysis; D005612:Freeze Drying / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy / Q000379:methods*; D011108:Polymers; D051381:Rats; D013696:Temperature
D000180:Acrylic Resins; D011108:Polymers; C037883:Lowicryl 4KM resin