Purification of a specific placental plasminogen activator inhibitor by monoclonal antibody and its complex formation with plasminogen activator.
A monoclonal antibody of IgG2a-type was obtained against a specific fast acting plasminogen activator inhibitor found in placenta. The placental inhibitor was purified by affinity chromatography using the monoclonal antibody and additionally in a FPLC-system. A strong complex formation was found between the inhibitor and urokinase and also with the two-chain form of plasminogen activator of the tissue-type. A weaker complex was found between the placental inhibitor and the one-chain form of the tissue-type activator.
D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D010920:Placenta / Q000032:analysis*; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators; D011247:Pregnancy; D014568:Urokinase-Type Plasminogen Activator
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D015849:Plasminogen Inactivators; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator