Isolation of germinal centre (GC) cells is greatly improved by using the protease dispase to prepare cell suspensions.
D001402:B-Lymphocytes / Q000473:pathology; D002469:Cell Separation; D010450:Endopeptidases; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D009157:Myasthenia Gravis / Q000276:immunology / Q000473:pathology*; D010950:Plasma Cells / Q000473:pathology; D011950:Receptors, Cholinergic / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology / Q000473:pathology*
D007074:Immunoglobulin G; D011950:Receptors, Cholinergic; D010450:Endopeptidases; C023139:dispase