A serum-free medium that supports the growth of cultured skeletal muscle satellite cells.
A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.
D000818:Animals; D001769:Blood; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D003907:Dexamethasone / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D009132:Muscles / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media; D007328:Insulin; D008041:Linoleic Acids; D005346:Fibroblast Growth Factors; D003907:Dexamethasone; D019787:Linoleic Acid