The regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum: cellular localization and developmental regulation analyzed by immunoblotting.
The level of the regulatory subunit of the cAMP-dependent protein kinase from Dictyostelium discoideum was analyzed in subcellular fractions of cells at various stages of development by Western blotting. The protein was found only in the cytosolic fraction. A small amount of regulatory subunit was present in vegetative cells, and its level increased sharply during the first hours of aggregation; a further increase also occurred during culmination. Analysis of mature spores and of the stalky mutant HL 65 revealed that the protein is present only in prespore cells.
D004023:Dictyostelium / Q000201:enzymology* / Q000502:physiology; D007163:Immunosorbent Techniques; D011494:Protein Kinases / Q000032:analysis / Q000378:metabolism*; D013172:Spores, Fungal / Q000201:enzymology
D011494:Protein Kinases