Detection of the microtubule cytoskeleton of the coccidian Toxoplasma gondii and the hemoflagellate Leishmania donovani by monoclonal antibodies specific for beta-tubulin.
Seven monoclonal antibodies specific for mammalian beta-tubulin demonstrate the microtubule cytoskeleton of Toxoplasma gondii and Leishmania donovani by indirect immunofluorescence microscopy. Immunoblots of T. gondii and L. donovani proteins separated by SDS polyacrylamide gel electrophoresis confirm the specificity of the monoclonal antibodies for tubulin. Differential staining of flagellar and subpellicular microtubule populations was not seen in L. donovani with these antibodies. All seven antibodies also detected the subpellicular microtubules of T. gondii, but the polar ring and conoid of this organism was not visualized by any of them. This technique provides a rapid and specific way to assess microtubular organization in whole organisms.
D000818:Animals; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D007893:Leishmania donovani / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D008870:Microtubules / Q000032:analysis* / Q000648:ultrastructure; D014122:Toxoplasma / Q000648:ultrastructure*; D014404:Tubulin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014404:Tubulin