Cloning of DNA sequences from Methanococcus vannielii capable of autonomous replication in yeast.
Total DNA of the archaebacterium Methanococcus vannielii was digested with BamHI or BamHI/HindIII, cloned with plasmid Yip5 and analyzed for sequences capable of autonomous replication (ARSs) in the eukaryote Saccharomyces cerevisiae. Two recombinant plasmids were isolated which contained 3.3 kb and 8 kb fragments of methanogen derived DNA with ARS activity. They exhibited low transformation efficiencies for yeast and promoted slow growth of yeast transformants.
D003001:Cloning, Molecular; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D019605:Euryarchaeota / Q000235:genetics*; D010957:Plasmids; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004269:DNA, Bacterial