Staining of the magnocellular nuclei of the rat hypothalamus by a monoclonal antibody directed against the alpha-subunit of the nicotinic cholinergic receptor.
Previous work has shown the vasopressin-secreting cells of the supraoptic nucleus receive a cholinergic input from a region dorsolateral to the nucleus, and that acetylcholine (ACh) activation of these cells is blocked by nicotinic antagonists. This work reports that a monoclonal antibody, directed against the alpha-subunit of the nicotinic ACh receptor isolated from Electrophorus electricus, stains certain hypothalamic cells and processes, particularly in regions associated with vasopressin-containing neurones.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D010121:Oxytocin / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013495:Supraoptic Nucleus / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism
D011978:Receptors, Nicotinic; D014667:Vasopressins; D010121:Oxytocin; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase