Morphology of transplanted tumours and drug induced regression in the subrenal capsule assay (SRCA) of mice and rats.
Specimens from freshly biopsied human mammary and ovarian tumours and DMBA-induced rat mammary tumours were transplanted under the renal capsule of normal, immunocompetent mice and rats. The growth of the tumour and the effects of chemotherapy to the host animals were evaluated by measuring the growth of the implanted tumour specimens. Morphological analysis of the tumours showed preserved histological structures and cellular function, as evidenced by the basement membrane component production seen using immunohistochemical stains and by the surface specialized structures, cell-to-cell contact and secretory activity observed in ultrastructural studies. Treatments with different types of drugs yielded tangible results within 6 days of transplantation, showing lymphoid infiltration and scar formation, but preserved vascular structures. The results suggest that tumours subjected to subrenal capsule assay retain their structure and function when transplanted, thus enabling the evaluation of their sensitivity to therapeutic manipulation.
D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D003114:Colony-Forming Units Assay / Q000379:methods*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014183:Transplantation, Heterologous; D014410:Tumor Stem Cell Assay / Q000379:methods*