[Determination, using a piezo-impulse method, of iso-osmotic permeability of the apical membrane of epithelium].
A frog skin, mechanically held on the mucosal side separates two Ringer solutions. It is submitted to an hydrostatic pressure difference delta P varying between 2 and 120 mb. Water permeability P (delta P) delta pi = 0 is determined with a piezo-impulse method. The rapid variation of permeability within the 2-25 mb range indicates a reversible closing of the junctions. The limiting value Pisol for high delta P is the isoosmotic permeability of the apical membrane.
D000818:Animals; D001001:Anura; D001692:Biological Transport; D001826:Body Fluids / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D008954:Models, Biological; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D012879:Skin Physiological Phenomena; D014882:Water-Electrolyte Balance
Détermination par une méthode piézoimpulsionnelle de la "perméabilité isoosmotique" de la membrane apicale d'un épithélium.