Acetylcholine stimulates a Ca2+-dependent C1- conductance in mouse lacrimal acinar cells.
Patch-clamp whole-cell current recordings under voltage-clamp conditions were carried out on isolated mouse exorbital lacrimal acinar cells. Acetylcholine evoked outward current at a membrane potential of -20 mV whereas an inward current was observed at -80 mV. The outward current is due to the well-known calcium-activated K+ channels whereas the inward current was C1- dependent. The acetylcholine-evoked C1- current was abolished when the intracellular Ca2+ concentration was clamped at very low levels by a high intracellular EGTA concentration. Acetylcholine therefore activates a Ca2+-dependent C1-conductance in mouse lacrimal acinar cells.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002712:Chlorides / Q000502:physiology*; D004533:Egtazic Acid / Q000494:pharmacology; D004553:Electric Conductivity; D007765:Lacrimal Apparatus / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D013268:Stimulation, Chemical; D013431:Sulfates / Q000502:physiology
D002712:Chlorides; D013431:Sulfates; D004533:Egtazic Acid; D000109:Acetylcholine; D002118:Calcium