Inhibition of aromatase and NADPH cytochrome c reductase activities in human endometrium by the human placental NADPH cytochrome c reductase antiserum.
The cross-reactivity of human placental microsomal NADPH-cytochrome c reductase antiserum, REDFBIV, against the endometrial reductase alone and as a component of the endometrial aromatase was investigated. Human endometrial particulate fractions were incubated with various amounts of REDFBIV for 1 h at 4 degrees C and both enzyme activities were measured at the end of incubation. The extent of inhibition of these endometrial enzymes was compared with the ability of this antiserum to inhibit the placental microsomal reductase and aromatase activities. The antiserum effectively inhibited the activities of both enzymes in both tissues in a dose dependent manner with aromatase activity inhibited to a greater extent than reductase activity. These results indicate the antiserum to the placental microsomal NADPH-cytochrome c reductase component of aromatase recognizes the reductase component of the aromatase enzyme system in endometrium.
D047072:Aromatase Inhibitors; D004717:Endometrium / Q000201:enzymology*; D004967:Estrogens / Q000378:metabolism; D005260:Female; D006801:Humans; D007106:Immune Sera; D009251:NADPH-Ferrihemoprotein Reductase / Q000037:antagonists & inhibitors* / Q000276:immunology; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013739:Testosterone / Q000378:metabolism
D047072:Aromatase Inhibitors; D004967:Estrogens; D007106:Immune Sera; D013739:Testosterone; D010088:Oxidoreductases; D009251:NADPH-Ferrihemoprotein Reductase