Human erythrocyte carbonic anhydrase I concentrations in dried blood spots from normal and hypothyroid neonates and children.
The ELISA technique was used to assay carbonic anhydrase I (HCAI) in hemolysates prepared by the elution of dried blood samples from Guthrie cards. The ratio HCAI (mg)/hemoglobin (g) measured in blood samples eluted from Guthrie cards was not significantly different from that determined in aliquots of the same blood samples after storage as erythrocyte (RBC) lysates at -20 degrees C, provided that the dried blood was eluted within three weeks of collection. The normal neonatal mean (SD) RBC HCAI concentrations were 2.05 (1.01) and 1.82 (0.86) mg HCAI/g hemoglobin for females and males respectively. Erythrocyte HCAI concentrations gradually rose with age, approaching normal adult levels by 16 years. Blood from hypothyroid neonates and hypothyroid infants on treatment gave normal HCAI/hemoglobin ratios.
D000293:Adolescent; D002256:Carbonic Anhydrases / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D007037:Hypothyroidism / Q000188:drug therapy / Q000201:enzymology*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012016:Reference Values; D013974:Thyroxine / Q000627:therapeutic use
D002256:Carbonic Anhydrases; D013974:Thyroxine