Liquid chromatographic determination of noxytiolin and 1-methyl-2-thiourea in serum: application to pharmacokinetic studies in rabbits and humans.
A high-performance liquid chromatographic method is described which determines noxytiolin and 1-methyl-2-thiourea concentrations in serum. Valid determination requires immediate ultracentrifugation of blood samples, rapid serum freezing, and injection into the chromatograph within 6 h. A number of pharmacokinetic parameters were calculated from serum concentration data in rabbits and humans. An unknown metabolite was detected in both species but its structure was not identified.
D000368:Aged; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005260:Female; D006801:Humans; D007274:Injections, Intraperitoneal; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009677:Noxythiolin / Q000008:administration & dosage / Q000097:blood*; D011817:Rabbits; D013890:Thiourea / Q000031:analogs & derivatives* / Q000097:blood
D009677:Noxythiolin; C002267:N-methylthiourea; D013890:Thiourea