The effect of streptozotocin-induced diabetes on hepatic hexose transport.
3-O-methyl-D-glucose (which is not metabolized in isolated parenchymal cells) was used to characterize the hexose transport process in hepatocytes prepared from 24 h fasted rats. The Vmax and Km obtained were 161 +/- 12 nmol/mg dry wt./min and 39 +/- 4 mM respectively (Europe-Finner GN, 1984, Biosci. Rep. 4, 483-489). Streptozotocin-induced diabetes decreased the Km of the system by 50% to a value of 19 +/- 6 mM without causing any change in the Vmax. Short term insulin treatment of cells prepared from 24 h diabetic rats appeared to partially return the system to normal.
D019325:3-O-Methylglucose; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005215:Fasting; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D051381:Rats
D007328:Insulin; D008757:Methylglucosides; D008759:Methylglycosides; D019325:3-O-Methylglucose