Ren-1 and Ren-2 loci are expressed in mouse kidney.
Inbred strains of mice can be categorized into two groups based on the absence or presence of a duplicated copy of the renin structural gene; one-gene strains carry a single renin gene (Ren-1), whereas two-gene strains carry two renin genes (Ren-1 and Ren-2). To investigate the contribution that each locus makes to the composite levels of renin mRNA observed to accumulate in different tissues of two-gene strains, we have developed two assays capable of distinguishing the highly homologous Ren-1 and Ren-2 transcripts. Both methods take advantage of established base sequence differences between Ren-1 and Ren-2 coding regions by using reverse transcriptase-mediated primer extension of oligonucleotide primer/mRNA hybrids in the presence of appropriate deoxy-and dideoxynucleotide phosphates. Using these techniques we found that Ren-1 and Ren-2 mRNAs accumulate in the kidney of two-gene strains to approximately equal levels. These observations are discussed in light of potential mechanisms regulating the tissue-specific expression of the Ren-1 and Ren-2 loci.
D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation; D006579:Heterozygote; D007527:Isoenzymes / Q000235:genetics; D007668:Kidney / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012083:Renin / Q000235:genetics*; D013363:Submandibular Gland / Q000201:enzymology; D014158:Transcription, Genetic
D007527:Isoenzymes; D012333:RNA, Messenger; D012083:Renin