Immuno-affinity purification of heparinase.
Polyclonal IgG rabbit antibodies were prepared against a purified heparinase from Flavobacterium heparinum. Immuno-affinity purification of crude and partially purified heparinase is described. The resulting enzyme was of comparable purity to that prepared using the standard multistep purification scheme. The antibodies prepared were found to increase the activity of bound heparinase.
D000818:Animals; D000917:Antibody Formation; D005417:Flavobacterium / Q000201:enzymology; D019763:Heparin Lyase; D007163:Immunosorbent Techniques; D011133:Polysaccharide-Lyases / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits
D011133:Polysaccharide-Lyases; D019763:Heparin Lyase