Investigation of a coagulation accelerating factor (CAF) in glomerulonephritis.
A coagulation accelerating factor was purified from the plasma of two patients with glomerulonephritis (GN) who suffered from thrombotic complications. The factor co-purified with factor VIII/von Willebrand factor complex (FVIII/vWf) and under dissociating conditions remained associated with the factor VIII coagulant activity (FVIII). Control purified FVIII/vWf showed no coagulation accelerating activity under the experimental conditions used. The levels of coagulation accelerating factor, FVIII and von Willebrand factor (vWf) were reduced by incubation with rabbit anti-human FVIII/vWf or human anti-FVIII serum indicating a close association of these three activities. Multimeric analysis of the plasma FVIII/vWf complex from the two patients demonstrated a reduction in the high molecular weight multimers and the presence of an additional band not present on analysis of normal FVIII/vWf. It is suggested that the coagulation accelerating factor represents an active form of FVIII which has different in vitro properties to thrombin activated FVIII.
D000328:Adult; D001779:Blood Coagulation Factors / Q000302:isolation & purification*; D003378:Counterimmunoelectrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005169:Factor VIII / Q000032:analysis / Q000302:isolation & purification; D005921:Glomerulonephritis / Q000097:blood* / Q000150:complications; D006801:Humans; D008297:Male; D013923:Thromboembolism / Q000097:blood* / Q000209:etiology; D014841:von Willebrand Factor / Q000032:analysis
D001779:Blood Coagulation Factors; D014841:von Willebrand Factor; D005169:Factor VIII