beta 2-Microglobulin secretion from lymphoid cells: a study at the cellular level.
A protein plaque assay was developed for the detection of human lymphocytes secreting beta 2-microglobulin. The development of plaques required production of beta 2-microglobulin by viable cells at an estimated rate exceeding 25 molecules per second. It has previously been suggested that beta 2-microglobulin secretion is restricted to certain lymphoid cell subpopulations. However, both T- and B-cells were shown to form plaques in normal donors and immunodeficient patients after activation with various mitogens. A close correlation between beta 2-microglobulin production and DNA synthesis was evident. However, secretion of immunoglobulin did not correlate with the proportion of cells secreting beta 2-microglobulin.
D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006462:Hemolytic Plaque Technique; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007153:Immunologic Deficiency Syndromes / Q000097:blood; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism
D007136:Immunoglobulins; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D001613:beta 2-Microglobulin; D004247:DNA