Gene expression at the pink-eyed dilution (p) locus in the mouse is confirmed to be pigment cell autonomous using recombinant embryonic skin grafts.
Sash (Wsh), a viable and fully fertile allele of the dominant spotting (W) locus (Lyon & Glenister, 1982) has been used in a modified test system to investigate the site of gene expression at the pink-eyed dilution (p) locus. Reciprocal recombinant epidermal@dermal skin grafts were constructed from 13-day embryonic skin of p and Wsh homozygotes. Thus in the reciprocal experiments pink-eyed dilution melanocytes were exposed to any environmental influence from the wild-type allele of the p locus in either the epidermis (when WshWsh) or the dermis (when WshWsh). The hair pigmentation of the grafts recovered after three weeks beneath the testicular tunica of adult male mice was always typical of the p phenotype showing that p is melanocyte autonomous. This result was supported by experiments using a modification of Mayer's (1965) neural crest grafting technique and the construction of 14-day recombinant skin grafts. Sash (WshWsh) epidermis can support melanocyte differentiation and pigment production but lacks functional melanocytes. The advantages of Wsh in experimental systems for testing the site of pigment gene expression have been demonstrated. Control experiments confirmed the dermal influence of agouti (A) over non-agouti (a) epidermis but non-agouti dermis did not overrule agouti pink-eyed dilution (AA pp) epidermis suggesting an epistatic effect of p in the melanocyte.
D000818:Animals; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D005838:Genotype; D008544:Melanocytes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009154:Mutation; D009432:Neural Crest / Q000637:transplantation; D010858:Pigmentation; D011995:Recombination, Genetic; D016038:Skin Transplantation