Photodynamic mutagenic action of acridine compounds on yeast Saccharomyces cerevisiae.
The photodynamically produced mutagenicity and toxicity of 8 acridine compounds were compared in Saccharomyces cerevisiae under resting and growing conditions. Without irradiation none of the acridines induced respiratory-deficient ('petite') colonies, indicative of mitochondrial DNA damage, in resting cells; and only acriflavine and proflavine induced 'petites' in growing cells. Also, without irradiation none of the acridines were significantly toxic or mutagenic for nuclear DNA under resting or growing conditions. However, with irradiation, acriflavine, proflavine, acridine yellow and rivanol became effective 'petite'-inducing mutagens and highly toxic for resting cells, while acriflavine, proflavine, and acridine orange became effective nuclear mutagens for resting cells. Acridine, quinacrine and 9-aminoacridine were not at all biologically effective with irradiation for resting cells. The results presented here indicate that singlet oxygen is generated by a photodynamic mechanism when acriflavine is irradiated, and further, that acridine, quinacrine and 9-aminoacridine are ineffective photosensitizers, because they are incapable of generating singlet oxygen with irradiation.
D000609:Aminoacridines / Q000494:pharmacology* / Q000528:radiation effects / Q000633:toxicity; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D009152:Mutagenicity Tests; D010100:Oxygen / Q000096:biosynthesis / Q000633:toxicity; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D026082:Singlet Oxygen; D013329:Structure-Activity Relationship
D000609:Aminoacridines; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D011838:Radiation-Sensitizing Agents; D026082:Singlet Oxygen; D010100:Oxygen