[Effect of Ecodam DNA-methylase on single-stranded sequences and synthetic oligonucleotides].
Interaction of the Ecodam methylase with different substrates were investigated among them the double- and single-stranded DNAs and synthetic oligonucleotides containing some defects in the GATC sequence. These defects were:nick, the absence of one internucleotide phosphate of nucleotide; partially single-stranded form on the recognition site etc. It was demonstrated that the presence of both G . A-dinucleotides in the recognition site is necessary for productive enzyme-substrate interaction. The absence of T and/or C residues is less dramatic for methylase activity. The Ecodam methylase is capable to modify the single-stranded oligonucleotides by forming the double-stranded structure in the symmetric recognition sequences GATC.
D000818:Animals; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D066298:In Vitro Techniques; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
Deĭstvie DNK-metilazy Ecodam na odnonitchatye posledovatel'nosti i sinteticheskie oligonukleotidy.