Fluorescence polarization applied to cellular microrheology.
The fluorescence polarization of probe molecules gives information on the "fluidity" of probe environment. Although the data cannot be related with absolute values of microviscosity, the method is largely used for probing the "fluidity" of lipid regions of biological membranes. Therefore, fluorescence polarization is of interest in clinical research, for membrane alterations are associated with either pathological processes of red cells, platelets, leukocytes or important cell functions.
D001792:Blood Platelets / Q000032:analysis; D004910:Erythrocyte Membrane / Q000032:analysis; D005454:Fluorescence Polarization / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D012212:Rheology; D013050:Spectrometry, Fluorescence
D008563:Membrane Lipids; D008565:Membrane Proteins