[Biological activity of thiophosphamide-alkylated DNA in the transformation system of Bacillus subtilis].
DNA treated with thiophosphamide was studied for changes in its biological activity in transformation system of Bac. subtilis. DNA alkylation by this three-functional alkylating agent is shown to be followed by the reduction of transforming activity, changes in the competitive ability as well as by a decrease of the cotransfer coefficient for linked markers of transforming DNA. The observed changes in the transforming and cotransforming activities may be explained by variations in the DNA structure.
D000478:Alkylation; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D007700:Kinetics; D009690:Nucleic Acid Conformation / Q000187:drug effects; D013329:Structure-Activity Relationship; D013852:Thiotepa / Q000494:pharmacology*; D014169:Transformation, Bacterial / Q000187:drug effects*
D004269:DNA, Bacterial; D013852:Thiotepa
Izuchenie biologicheskoĭ aktivnosti DNK, alkilirovannoĭ tiofosfamidom v transformatsionnoĭ sisteme Bac. subtilis.