Rapid yeast DNA staining method for flow cytometry.
The variation of the fluorescence intensity of olivomycin-stained yeast cells as a function of the concentration of olivomycin, NaCl, and MgCl2 in the staining solution was studied. The best results were obtained when the staining solution contained 100 micrograms, olivomycin/ml, 40 mM MgCl2, and 1 M NaCl. A staining time of 12 min was sufficient for proper staining.
D004271:DNA, Fungal / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D008274:Magnesium; D015636:Magnesium Chloride; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D012965:Sodium Chloride
D004271:DNA, Fungal; D015636:Magnesium Chloride; D012965:Sodium Chloride; D008274:Magnesium