Identification of gastrin-producing cells in cell cultures and smears: an avidin-biotin-peroxidase complex (ABC) technique.
A method is described that uses the avidin-biotin complex (ABC) immunoperoxidase technique to provide a rapid, sensitive, and specific means to quantitate isolated G cells in cultures and suspensions.
D000818:Animals; D001360:Avidin; D001710:Biotin; D002478:Cells, Cultured; D005404:Fixatives; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D005755:Gastrins / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011706:Pyloric Antrum; D051381:Rats; D011919:Rats, Inbred Strains; D015043:Zollinger-Ellison Syndrome / Q000473:pathology
D005404:Fixatives; D005755:Gastrins; D001360:Avidin; D001710:Biotin