Inhibition of gluconeogenesis by 2,5-anhydro-D-mannitol in isolated rat hepatocytes.
2,5-Anhydro-D-mannitol inhibited glucose synthesis, increased the pyruvate/phosphoenolpyruvate ratio and altered adenine nucleotide concentrations in hepatocytes isolated from fasted rats. The accumulations of 2,5-anhydro-D-mannitol 1,6-diphosphate, an allosteric activator of pyruvate kinase, and of ADP in treated hepatocytes can account for the increase in pyruvate/phosphoenolpyruvate ratio and the inhibition of glucose synthesis from lactate.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D005215:Fasting; D005943:Gluconeogenesis / Q000187:drug effects*; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008353:Mannitol / Q000031:analogs & derivatives* / Q000494:pharmacology; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000227:Adenine Nucleotides; D007773:Lactates; D019344:Lactic Acid; D008353:Mannitol; C031765:2,5-anhydromannitol; D011770:Pyruvate Kinase