The fluorometric determination of europium ion concentration as used in time-resolved fluoroimmunoassay.
A model of the relationships between optimal fluorescence yield, Eu3+ ion concentration, and the concentration of beta-diketone in the determination of Eu3+ ion concentration in aqueous solutions as used in time-resolved fluoroimmunoassays was developed. The model, in addition to optimizing the determination of the metal ion, also presents a new simple and rapid method for the determination of stability constants for the formation of beta-diketone-Eu3+ complexes. It is shown how the method may be extended to determine the formation constants for Eu3+ with other chelates.
D002614:Chelating Agents / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D004492:Edetic Acid; D005063:Europium / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods; D007659:Ketones / Q000032:analysis; D008433:Mathematics; D008956:Models, Chemical; D009943:Organophosphorus Compounds; D013050:Spectrometry, Fluorescence
D002614:Chelating Agents; D007659:Ketones; D009943:Organophosphorus Compounds; D005063:Europium; C044965:trioctyl phosphine oxide; D004492:Edetic Acid