Immunological detection of cloned antigenic genes of Vibrio cholerae in Escherichia coli.
Antibodies have been used as probe to detect cloned genes coding for toxin and surface antigens of Vibrio cholerae El Tor strain KB207. EcoRI-digested chromosomal DNA of KB207 was cloned in plasmid pBR325 and transformed in Escherichia coli HB101(lambda cI857). Transformants were grown at 32 degrees C on plates containing antibodies. Lysogen was induced at 42 degrees C to release expressed antigens. Antigen-antibody reaction produced a halo around positive clones.
D000942:Antigens, Bacterial / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids; D014734:Vibrio cholerae / Q000235:genetics*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D004274:DNA, Recombinant