Characterization of hemopoietic growth factors from T cells and the myelomonocytic leukemia WEHI-3B.
P-cell-stimulating factor (PSF) (also termed interleukin 3) produced by the T-cell clone A3-37.4, the T-cell hybridoma 123, the T-lymphoma EL4, spleen cells, and the myelomonocytic cell line WEHI-3B had a similar apparent mol. wt. and in each case eluted from a Waters C18 silica column at a concentration of acetonitrile of 38%. Both the PSF from the T-cell clones and from WEHI-3B stimulated the in vitro growth of cloned T-dependent mast cells and of colonies from normal bone marrow cells. The T-cell sources--but not WEHI-3B--also produced an additional, distinct hemopoietic growth factor that stimulated the growth of colonies of neutrophils and macrophages but did not support the growth of P cells. This factor was termed T-cell granulocyte-macrophage colony-stimulating factor (T-cell GM-CSF). T-cell GM-CSF eluted from a C18 silica column at an acetonitrile concentration of 41%, differing in this respect from both PSF, which eluted at 38% acetonitrile, and the GM-CSF produced by endotoxin-stimulated mouse lungs.
D000818:Animals; D002460:Cell Line; D006133:Growth Substances / Q000302:isolation & purification; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D007525:Isoelectric Focusing; D007942:Leukemia, Experimental / Q000378:metabolism / Q000473:pathology; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D013601:T-Lymphocytes / Q000378:metabolism*
D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3