Solid-phase ABO grouping and Rh typing.
A solid-phase adherence method (SPAM) for ABO grouping and Rh typing of red cells (RBCs) has been developed. Adherence reactions were read spectrophotometrically and interpreted by a computer. The SPAM had a 99.6 percent correlation with conventional microplate agglutination methods for ABO grouping and Rh typing. The increased sensitivity of the SPAM was demonstrated because it directly detected Du-positive RBCs and weak subgroups of A and B.
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001322:Autoanalysis; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D008838:Microcomputers; D012204:Rh-Hr Blood-Group System
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D012204:Rh-Hr Blood-Group System