Cloning of the pectate lyase genes from Erwinia carotovora and their expression in Escherichia coli.
A hybrid cosmid coding for pectate lyase (PL) activity was identified from an Erwinia carotovora genomic library by an immunological screening method. A 7-kb DNA fragment was identified which codes for three proteins identical in size to proteins with PL activity purified from E. carotovora culture supernatants. The three proteins had apparent Mrs of 41, 44 and 44 X 10(3) as estimated by SDS-PAGE. None of the PLs were exported from Escherichia coli strain HB101 but all were found in the periplasmic space. Plant tissue was macerated by the PLs made in E. coli.
D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004885:Erwinia / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D010957:Plasmids; D011133:Polysaccharide-Lyases / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D011133:Polysaccharide-Lyases; C023911:pectate lyase