Controlled transcription of the yeast regulatory gene GAL80.
Transcription of the tightly linked genes GAL7-GAL10-GAL1 encoding three galactose-metabolizing enzymes in Saccharomyces cerevisiae is regulated by an interplay of the positive regulatory gene GAL4 and the negative regulatory gene GAL80. The concentration of GAL80 mRNA (determined by a quantitative blot hybridization) was nearly comparable to that of URA3 mRNA, in the wild-type yeast grown in medium with glucose or glycerol. The intracellular concentration of GAL80 mRNA increased by changing the carbon source to galactose by a factor of more than 5, in contrast to a GAL4 mRNA concentration which is essentially unaffected by galactose [Laughon and Gesteland, Proc. Natl. Acad. Sci. USA 79 (1982) 6827-6831]. The inducible expression of the GAL80 gene was suggested to involve its own product and also the product of GAL4 by using various mutations in those genes.
D004271:DNA, Fungal / Q000235:genetics; D005686:Galactokinase / Q000235:genetics; D005690:Galactose / Q000494:pharmacology; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005809:Genes, Regulator; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012331:RNA, Fungal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D014534:UDPglucose 4-Epimerase / Q000235:genetics; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase / Q000235:genetics
D004271:DNA, Fungal; D012331:RNA, Fungal; D005686:Galactokinase; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase; D014534:UDPglucose 4-Epimerase; D005690:Galactose