A direct evidence of the localization of mitochondrial calmodulin.
The presence and localization of mitochondrial calmodulin was directly proved immuno-electron microscopically by the protein A-gold technique. In the ultra-pure mitochondria the complexes of anti-calmodulin antibody and protein A-gold clearly showed the localization of mitochondrial calmodulin on the inner membrane and in the matrix space.
D000818:Animals; D002147:Calmodulin / Q000032:analysis* / Q000276:immunology; D006046:Gold / Q000378:metabolism; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000032:analysis*; D051381:Rats; D013205:Staphylococcal Protein A / Q000378:metabolism
D002147:Calmodulin; D013205:Staphylococcal Protein A; D006046:Gold