A sperm-agglutinating lectin from seeds of Jack fruit (Artocarpus heterophyllus).
A lectin specific for N-acetylgalactosamine was isolated from seed extract of Jack fruit (Artocarpus heterophyllus) by ammonium sulfate precipitation, followed by affinity chromatography on a Affigel-galactosamine-agarose column. The lectin possessed agglutinating activities for human and rat sperm as well as human red blood cells. It was found to have Mr = 62,000 consisting of two dissimilar subunits of Mr = 18,000 and 13,000. It also cross-reacted with an antibody against the lectin of Osage Orange (Maclura pomifera).
D000116:Acetylgalactosamine / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D005779:Immunodiffusion; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D037121:Plant Lectins; D051381:Rats; D012639:Seeds / Q000032:analysis; D013073:Sperm Agglutination / Q000187:drug effects*
D000596:Amino Acids; D005960:Glucosides; D037102:Lectins; D037121:Plant Lectins; C018619:octyl-beta-D-glucoside; D000116:Acetylgalactosamine; D005690:Galactose