[Frequency of methicillin resistance varies with conditions, in a population of staphylococci from a hospital environment].
The incidence of methicillin-resistant Strains is increasing in these last years. This increase is particularly evident in Strains isolated from hospital environments. In this work we evaluated the methicillin-resistance of 58 Staphylococcus Strains, isolated from a surgical intensive care ward, in various experimental conditions, which changing in: culture medium, inoculum, incubation period and antibiotic concentration. Besides this we took in consideration various factors which can influence the frequency of methicillin-resistance and can also lead to errors in the evaluation itself. From our study we can conclude that the factors are: survey "in vitro" of the resistance or of the sensitivity, exclusion or inclusion of staphylococci different from Staphylococcus aureus, double count of the same Strains isolated in different times.
D001431:Bacteriological Techniques; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007362:Intensive Care Units; D008712:Methicillin / Q000494:pharmacology*; D010403:Penicillin Resistance; D013210:Staphylococcus / Q000187:drug effects*
D003470:Culture Media; D008712:Methicillin
Frequenze di meticillino-resistenza e variabili che le modificano, su una popolazione di stafilococchi di provenienza ospedaliera.