A competitive solid-phase radioimmunoassay for quantitation of the major allergen of Parietaria pollen.
A competitive solid-phase radioimmunoassay has been developed for quantitation of the major allergen of Parietaria judaica pollen. The assay is based on: (1) the ability of AC/1.1 monoclonal antibody to bind specifically to the P. judaica major allergen, and (2) the ability of crude pollen extracts or purified allergen to inhibit the binding of 125I-labelled allergen to solid-phase-bound AC/1.1 monoclonal antibody. The assay is sensitive enough to detect as little as 10 ng of allergen. A good correlation is found when the results obtained are compared with those produced by RAST inhibition (r = 0.95; P less than 0.001). Thus, this method can also be used for the estimation of the allergenic activity of P. judaica pollen extracts. The assay is easily completed in 2 h, allowing simultaneous analysis of a number of extracts.
D000485:Allergens / Q000032:analysis*; D000911:Antibodies, Monoclonal; D006801:Humans; D007163:Immunosorbent Techniques; D010944:Plants / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods
D000485:Allergens; D000911:Antibodies, Monoclonal