[Synthesis of lipid A analogs. Obtaining conjugates of 6-phosphate 2-desoxy-2-(3-hydroxymyristoyl)amino-D-glucose with polymer carriers and their immunological properties].
The derivatives of 2-acylamino-6-O-(2-aminoethyl) phosphono-3-deoxy-D-glucose acylated with acetic or D,L-3-hydroxytetradecanoic acid were obtained, and their 31P-and 13C-NMR spectra investigated. These haptens were bound with a polysaccharide (Ficoll) or proteins (albumins, bovine gamma-globulin). The protein conjugates were immunogenic in rabbits, specific antibodies against the hapten being revealed by two immunochemical methods. As shown by the enzyme-linked immunoadsorbent assay, the specific rabbit antiserum reacted with lipid A from Yersinia pseudotuberculosis.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology; D006241:Haptens / Q000276:immunology; D007114:Immunization; D008050:Lipid A / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology; D009227:Myristic Acids / Q000138:chemical synthesis* / Q000276:immunology; D011108:Polymers; D011817:Rabbits; D015007:Yersinia / Q000276:immunology*; D015012:Yersinia pseudotuberculosis Infections / Q000382:microbiology
D006241:Haptens; D008050:Lipid A; D009227:Myristic Acids; D011108:Polymers; C046464:2-deoxy-2-(3-hydroxytetradecanoyl)aminoglucose 6-phosphate; D005944:Glucosamine
Sintez analogov lipida A. Poluchenie kon'iugatov 6-fosfata 2-dezoksi-2-(3-oksimiristoil) amino-D-gliukozy s polimernymi nositeliami i ikh immunologicheskie svoistva.