Rapid assay of N-butyl-N-(3-carboxypropyl)nitrosamine in rat organs and urine by high-performance liquid chromatography after derivatization.
The concentration of N-butyl-N-(3-carboxypropyl)nitrosamine (BCPN), which is the major metabolite of the carcinogen N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN), was measured in the urine, thymus, liver, kidney, and bladder of rats orally administered with BBN. Since BCPN is a carboxylic acid, it forms an ester with 9-anthryldiazomethane (ADAM), which is a fluorescent labeling agent highly sensitive to carboxylic acids. Thus, BCPN and ADAM were reacted at 40 degrees for 1 hr, and the resulting ester was separated and measured by high-performance liquid chromatography (HPLC) with a reverse-phase type column. The range of measurement was 0 to 40 micrograms/ml, and the coefficient of variation (CV) was 3.8%. When 0.025% BBN was given orally to rats in tap water, the BCPN concentration in the urine was very high at 220 micrograms/ml, while it was 0.15 microgram/100 mg in the wet tissues of the thymus, 0.35 microgram/100 mg in the liver, 0.40 microgram/100 mg in the kidney, and 1.2 microgram/100 mg in the bladder. The BCPN concentration in the bladder, in which tumors are induced by the administration of BBN, was thus higher than those in the other organs.
D000818:Animals; D002085:Butylhydroxybutylnitrosamine / Q000008:administration & dosage / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D009602:Nitrosamines / Q000032:analysis* / Q000652:urine; D051381:Rats; D013950:Thymus Gland / Q000032:analysis*; D001743:Urinary Bladder / Q000032:analysis*
D009602:Nitrosamines; D002085:Butylhydroxybutylnitrosamine; C006853:butyl(3-carboxypropyl)nitrosamine