[Preparation and use of magnetic sorbents for studying microorganism antigens].
In this work simple techniques for obtaining polyacrylamide sorbents with magnetic properties are described. These techniques have permitted obtaining block and microgranulated sorbents with the immobilization of antibodies from plague antiserum in the cellular gel structure for the specific sorption of killed and live Yersinia pestis cells and their first fraction; pig brain gangliosides have also been incorporated into the gel structure with a view to the sorption of cholera toxin from the filtrate of Vibrio cholerae culture. The magnetic properties of sorbents, obtained by the copolymerization of powdered magnetic ferric oxides in gel, have made it possible to increase the effectiveness of specific sorption due to mixing and rapid separation in different magnetic fields, as well as to facilitate and accelerate manipulations with the sorbent at all stages. The capacity of different types of sorbents and the time of sorption have been determined.
D000180:Acrylic Resins; D000327:Adsorption; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000032:analysis*; D004655:Emulsions; D005732:Gangliosides; D005782:Gels; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000302:isolation & purification*; D008024:Ligands; D008280:Magnetics; D011208:Powders; D012995:Solubility; D014734:Vibrio cholerae / Q000276:immunology*; D015010:Yersinia pestis / Q000276:immunology*
D000180:Acrylic Resins; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D004655:Emulsions; D005732:Gangliosides; D005782:Gels; D007164:Immunosorbents; D008024:Ligands; D011208:Powders; C016680:polyacrylamide gels
Prigotovlenie i primenenie magnitnykh sorbentov dlia izucheniia antigenov mikroorganizmov.