The effects of insulin and concanavalin A on the accumulation of a specific mRNA in rat hepatoma cells.
One of insulin's effects is to stimulate specific mRNA synthesis. Treatment of H4IIE hepatoma cells with 0.01-1.0 nM insulin results in a maximum 10-15 fold increase in the accumulation of a specific mRNA (p33-mRNA) as measured with a cloned cDNA. Concanavalin A, a lectin known to mimic many of insulin's effects, also stimulates the accumulation of p33-mRNA. The effects of both insulin and Con A were blocked by the addition of two RNA synthesis inhibitors, actinomycin D or 5,6 dichloro-1-beta-D-ribofuranosyl-benzimidazole. We therefore suggest that insulin and concanavalin A act to stimulate p33-mRNA synthesis.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D051381:Rats
D007328:Insulin; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D003208:Concanavalin A