Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli.
A strain of Escherichia coli was isolated in which dihydrofolate reductase was not detected by an enzyme assay or by competition for antibody. This strain requires methionine, glycine, a purine, and thymidine for growth in addition to the auxotrophic requirements of the parent strain. It was found to be useful as a recipient of plasmids harboring dihydrofolate reductase genes.
D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D009154:Mutation; D013762:Tetrahydrofolate Dehydrogenase / Q000032:analysis / Q000172:deficiency* / Q000235:genetics
D013762:Tetrahydrofolate Dehydrogenase