Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver.
The complete amino acid sequence of the cysteine proteinase inhibitor cystatin B (formerly named CPI-B) from human liver was determined. The 98-residue sequence (Mr = 11,175) was obtained by automated solid-phase Edman degradation of a large cyanogen bromide fragment and peptides generated by enzymatic cleavage. The protein starts with a blocked Met-Met sequence which is presumably N-acetylated. The sequence shows that human cystatin B is a member of the family of intracellular cystatins; it is 79% identical with cystatin beta from rat liver, but contains only a single cysteine. Human cystatin B is able to form a dimer stabilized by noncovalent forces.
D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide; D055313:Cystatin B; D015891:Cystatins; D003546:Cysteine Endopeptidases; D006801:Humans; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism; D011480:Protease Inhibitors; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013045:Species Specificity
C524994:CSTB protein, human; D015891:Cystatins; D011480:Protease Inhibitors; D011506:Proteins; D055313:Cystatin B; D003546:Cysteine Endopeptidases; D003488:Cyanogen Bromide