Inactivation of key factors of the plasma proteinase cascade systems by Bacteroides gingivalis.
The effect of Bacteroides gingivalis W83 on various key components of the human plasma proteinase cascade systems was studied. When purified C1-inhibitor was incubated with the bacterium, the inhibitor was rapidly inactivated by limited proteolytic cleavage. In citrated whole plasma, C1-inhibitor, antithrombin, plasminogen, prekallikrein, prothrombinase complex, the clotting factor X, and most of the alpha 2-antiplasmin were functionally eliminated after 30 min of incubation with the bacterium. Fibrinogen disappeared from the plasma almost immediately upon mixing with the bacterial suspension. In contrast, there was no appreciable decrease in the bulk of other plasma proteins, such as various transport proteins (albumin, prealbumin, transferrin) and immunoglobulins, during 4 h of incubation with the bacterium. Most of the observed effects can be assigned to the proteolytic activity of the bacterium itself, since there was little evidence for generation of intrinsic plasma proteinase activity, despite the loss of proteinase inhibitory activities. B. gingivalis W83 thus seems to be equipped with proteolytic enzyme systems which selectively recognize and rapidly inactivate the most important proteinase inhibitors and proenzymes present in human plasma. This bacterium therefore seems to be able to efficiently paralyze the host's various defenses against invading microorganisms.
D001439:Bacteroides / Q000201:enzymology*; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism; D003174:Complement C1 Inactivator Proteins / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005165:Factor V / Q000378:metabolism; D005170:Factor X / Q000378:metabolism; D015951:Factor Xa; D006801:Humans; D007700:Kinetics; D010447:Peptide Hydrolases / Q000097:blood* / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism; D011288:Prekallikrein / Q000378:metabolism; D011480:Protease Inhibitors
D001798:Blood Proteins; D003174:Complement C1 Inactivator Proteins; D011480:Protease Inhibitors; C022475:prothrombinase complex; D005165:Factor V; D005170:Factor X; D010958:Plasminogen; D011288:Prekallikrein; D010447:Peptide Hydrolases; D015951:Factor Xa