New model systems to study DNA-protein recognition mechanisms.
dpG antibodies were fractionated on a cellulose-double-stranded DNA column. The flow-through fraction bound denatured DNA but not double-stranded DNA (dsDNA). The dpG-eluted fraction bound dsDNA preferentially. The results show that proteins can recognise dpG in DNA by different mechanisms, some involving DNA unwinding.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D004247:DNA / Q000276:immunology*; D004269:DNA, Bacterial / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D006241:Haptens / Q000276:immunology*; D009691:Nucleic Acid Denaturation; D011817:Rabbits
D000906:Antibodies; D004269:DNA, Bacterial; D006241:Haptens; D004247:DNA