[Use of beta-lactamase from Bacillus licheniformis 749/C for solid-phase immunoenzyme analysis].
beta-Lactamase from Bacillus licheniformis 749/c was used as a marker for ELISA. Conjugates of the beta-lactamase with the capsule antigen of the plague causative agent and with the monoclonal antibodies to it were prepared by glutaric aldehyde "linking". The conjugates preserved high immunospecific and beta-lactamase activity. High sensitivity of the modification of ELISA allowed detecting up to 8 ng/ml of the capsule antigen of the plague causative agent. The enzymatic activity of the beta-lactamase was determined microiodometrically which provided visual recording of the results of ELISA.
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001407:Bacillus / Q000201:enzymology*; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007202:Indicators and Reagents; D015010:Yersinia pestis / Q000276:immunology; D001618:beta-Lactamases
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007202:Indicators and Reagents; D001618:beta-Lactamases
Ispol'zovanie beta-laktamazy iz Bacillus licheniformis 749/C dlia tverdofaznogo immunofermentnogo analiza.