Application of ELISA-inhibition to Aspergillus antigen standardization for immunodiagnosis.
An inhibition ELISA method was used to test culture filtrate antigens of Aspergillus fumigatus for the avidity of antibodies to them. Four different culture media were used to check the ability of this assay to distinguish between similar antigenic preparations. Differences between antigens exhibiting similar crossed immunoelectrophoresis patterns were observed by inhibition ELISA.
D000908:Antibodies, Fungal / Q000032:analysis; D000915:Antibody Affinity; D000946:Antigens, Fungal / Q000032:analysis* / Q000592:standards; D001228:Aspergillosis / Q000175:diagnosis*; D001232:Aspergillus fumigatus / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007122:Immunoelectrophoresis; D012698:Serologic Tests / Q000592:standards
D000908:Antibodies, Fungal; D000946:Antigens, Fungal