A gel casting technique allowing rapid and sensitive quantitation of antibodies by diffusion-in-gel enzyme-linked immunosorbent assay.
A technique is described which allows rapid and sensitive quantitation of antibodies by diffusion towards an antigen-coated surface through a gel of wedge-shaped profile. A sensitivity of 0.16 micrograms antibody per ml solution was obtained after 45 min incubation with stirring at 22 degrees C and 0.08 micrograms/ml could be detected after 90 min incubation with stirring or with incubation at 37 degrees C.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006735:Horseradish Peroxidase; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D010047:Ovalbumin / Q000276:immunology; D011817:Rabbits; D013997:Time Factors
D000906:Antibodies; D010047:Ovalbumin; D006735:Horseradish Peroxidase