[Isolation of the internal proteins of the influenza virus by preparative polyacrylamide gel electrophoresis for obtaining monospecific antisera].
The principal possibility of isolation of internal proteins (M and NP) of influenza type A (H1N1 and H3N2) and B viruses by SDS-PAG preparative electrophoresis and preparation of monospecific antisera to these proteins was demonstrated. The resulting preparations may be used for testing the biological objects by enzymeimmunoassay.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007106:Immune Sera / Q000302:isolation & purification*; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D009981:Influenza B virus / Q000276:immunology / Q000302:isolation & purification*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D011817:Rabbits; D014758:Viral Core Proteins / Q000276:immunology / Q000302:isolation & purification*; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification
D000956:Antigens, Viral; D007106:Immune Sera; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins
Vydelenie vnutrennikh belkov virusa grippa metodom preparativnogo élektroforeza v poliakrilamidnom gele dlia polucheniia monospetsificheskikh antisyvorotok.