Monoclonal antibodies against species-specific cephalosporinase of Pseudomonas aeruginosa.
Ten hybridomas which produced monoclonal antibodies against species-specific cephalosporinase of Pseudomonas aeruginosa were constructed by somatic cell fusion of SP-2/0 myeloma cells with spleen cells from hyperimmunized BALB/c mice and intraperitoneally injected into mice. Monoclonal antibodies were partially purified from ascites fluids. All ten antibodies thus prepared were IgG immunoglobulins. In a solid-phase immunoassay the antibodies gave positive reactions which could be prevented by the presence of free cephalosporinase. When the antibodies were precipitated with anti-(mouse IgG), the cephalosporinase activity was co-precipitated. Also, these antibodies were co-eluted with cephalosporinase activity in gel filtration. Nine of the monoclonal antibody preparations elevated the cephalosporinase activity by about 6-40% and only one inhibited it by about 50%. All the monoclonal antibodies were highly specific to P. aeruginosa cephalosporinase and showed no cross-reaction with nine cephalosporinases and four penicillinases of other gram-negative bacteria.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002510:Cephalosporinase / Q000276:immunology*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006090:Gram-Negative Bacteria / Q000201:enzymology; D007120:Immunochemistry; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013045:Species Specificity; D001618:beta-Lactamases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D002510:Cephalosporinase; D001618:beta-Lactamases