Turnover of the cell wall peptidoglycan during growth of Neisseria gonorrhoeae and Escherichia coli. Relative stability of newly synthesized material.
The peptidoglycan of a number of strains of Neisseria gonorrhoeae and Escherichia coli turned over during exponential growth as monitored by the loss of radioactivity (supplied as [14C]glucosamine) from SDS-insoluble material. However, no turnover of the peptide side chains of E. coli peptidoglycan was observed (monitored by diamino[3H]pimelic acid) even though turnover of glycan material was occurring. Turnover rates of 9 to 15% per generation were recorded for all the N. gonorrhoeae strains studied except for the autolytic variant RD5 which showed a higher rate of turnover (20 to 26% per generation). In contrast to previous interpretations, these rates of turnover were not affected by benzylpenicillin, unless sufficient antibiotic was present to affect culture turbidity, when lysis occurred. Examination of the fragments (monomer, dimer and their O-acetylated counterparts, and oligomers) produced by Chalaropsis B muramidase treatment of prelabelled peptidoglycan revealed that no fraction of the peptidoglycan was immune from turnover. However, peptidoglycan pulse-labelled for only 10 min did not show immediate turnover. The lapse of time before turnover commenced was strain dependent, with a maximum value of 1.5 generations. This work confirms that the peptidoglycan of N. gonorrhoeae undergoes a period of maturation and suggests that only mature peptidoglycan turns over.
D001426:Bacterial Proteins / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D004926:Escherichia coli / Q000254:growth & development*; D009344:Neisseria gonorrhoeae / Q000254:growth & development*; D010400:Penicillin G / Q000494:pharmacology; D010457:Peptidoglycan / Q000378:metabolism*
D001426:Bacterial Proteins; D010457:Peptidoglycan; D010400:Penicillin G