[Expression of the HBsAg gene of the hepatitis B virus under the control of the promoter region of gene PH05 in yeast cells].
Recombinant plasmids have been constructed containing the complete HBsAg gene combined with the promotor region of the yeast gene of acid phosphatase. The plasmids are capable of replication in E. coli and Saccharomyces cerevisiae cells. In cultivation of yeast cells containing these plasmids in a synthetic medium with a low phosphorus content a protein is synthesized possessing the properties of hepatitis B virus surface antigen.
D000135:Acid Phosphatase / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005814:Genes, Viral; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D004274:DNA, Recombinant; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D000135:Acid Phosphatase
Ekspressiia gena HBsAg virusa gepatita B pod kontrolem promotornoĭ oblasti gena PH05 v kletkakh drozhzheĭ.