Influence of MAO A and MAO B on the inactivation of noradrenaline in the saphenous vein of the dog.
Specific inhibitors of MAO A (clorgyline 0.1 mumol/l) and of MAO B [(-)deprenyl 10 mumol/l] were used in dog saphenous vein strips in order to study the relative influence of the two types of MAO on: 1. Termination of contractile response to exogenous noradrenaline (NA); 2. Metabolism and accumulation of exogenous (3H)-NA; 3. Metabolism of 3H-NA released by electrical stimulation. To study the termination of contractile response to exogenous NA, the oil immersion technique was used to determine the time for half-relaxation. The experiments were performed on strips with or without treatment with cocaine 10 mumol/l (to inhibit neuronal uptake) plus U-0521 100 mumol/l (to inhibit catechol-O-methyl transferase) before and after exposure to MAO inhibitors. Clorgyline, but not (-)deprenyl enhanced significantly the time for half-relaxation of the strips, whether cocaine was present or not. To study the metabolism and accumulation of exogenous 3H-NA, the strips were incubated (with or without preincubation with cocaine) with 3H-NA 0.23 mumol/l and 2.3 mumol/l in the presence and in the absence of MAO inhibitors. The formation of deaminated metabolites was significantly reduced by clorgyline, but not by deprenyl. To study the metabolism of 3H-NA released by electrical stimulation, the strips were incubated with 3H-NA 1.4 mumol/l. In the presence of cocaine and U-0521, field stimulation was applied during two periods of 5 min (10 Hz, 100 V, 2 ms), in the absence or presence of MAO inhibitors. Under these experimental conditions clorgyline, but not deprenyl, abolished DOPEG formation, without affecting the other metabolites.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D003010:Clorgyline / Q000494:pharmacology; D003641:Deamination; D004285:Dogs; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008995:Monoamine Oxidase / Q000494:pharmacology*; D009638:Norepinephrine / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012501:Saphenous Vein / Q000378:metabolism*; D012642:Selegiline / Q000494:pharmacology
D012642:Selegiline; D008995:Monoamine Oxidase; D003010:Clorgyline; D009638:Norepinephrine