Enterotoxigenicity of clinical isolates of non-O1 Vibrio cholerae.
Whole cultures, but not culture supernatant fluids, of 21 isolates of non-O1 V. cholerae from patients with diarrhea were shown to induce positive fluid accumulation in infant mice. CHO cell assays demonstrated the elaboration of heat-labile cytotonic, cytotoxic or both factors from most isolates when grown under optimal culture conditions. These factors were not neutralized by anti-cholera toxin serum. Also genetic studies performed on 9 vibrio isolates using a DNA hybridization probe failed to detect gene sequences homologous with cholera toxin. ELISA assays recognized six isolates which produced a cell-associated substance which immunologically cross-reacted with cholera toxin. Enzymatic profiles of the vibrio isolates did not correlate with the production of any toxic factor. The results indicate that mainly heat-labile and cell-associated cytotonic and cytotoxic factors appear to influence the enterotoxigenic potential of this heterogenous group of non-O1 vibrios.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002771:Cholera / Q000382:microbiology*; D006224:Cricetinae; D003412:Cricetulus; D004768:Enterotoxins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D012703:Serotyping; D014734:Vibrio cholerae / Q000472:pathogenicity*; D014774:Virulence
D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae