LH-RH in the rat and mouse hypothalamus and rat hypophysial portal blood: confirmation of identity by high performance liquid chromatography.
The nature of the immunoreactive (IR) form of luteinizing hormone-releasing hormone (LH-RH) in rat hypophysial portal vessel blood and in extracts of hypothalamus from rats and normal and hypogonadal (hpg) mice was investigated using high performance liquid chromatography and two highly specific anti-LH-RH sera. In rat hypophysial portal blood and in hypothalamic extracts from rats and normal mice a single immunoreactive peak was present which corresponded in retention time to synthetic LH-RH. No LH-RH-IR was detected in hypothalamic extracts from the hpg strain of mouse.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis* / Q000097:blood; D007006:Hypogonadism / Q000235:genetics / Q000378:metabolism*; D007030:Hypothalamo-Hypophyseal System / Q000098:blood supply*; D007031:Hypothalamus / Q000032:analysis*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D051381:Rats; D011919:Rats, Inbred Strains
D007987:Gonadotropin-Releasing Hormone