In vivo proteolytic activity of the mammary gland. Contribution to the origin of secretory component, beta 2-microglobulin and bovine-associated mucoprotein (BAMP) in cows milk.
Milk samples were collected from Holstein-Friesian cows at various times after milking (10-30 min; 30 min-10 hr) and treated with a protease inhibitor or control solution. Samples were then fractionated into whole, skimmed and cell-free skimmed milk aliquots. Some animals were treated with E. coli endotoxin prior to sample collection. The concentrations of three membrane-associated proteins (MAP), beta 2-microglobulin (beta 2M), secretory component (SC) and bovine-associated mucoprotein (BAMP) as well as albumin were measured in each aliquot to determine if in vivo proteolysis of milk elements could explain the origin of these MAP in milk. All three MAP could be localized on milk fat globules (MFG) and alveolar epithelial cells of the gland. Data revealed that all BAMP in milk can be accounted for by in vivo proteolytic degradation of MFG while most beta 2M is derived by similar degradation, from cellular elements in milk, presumably monocytes. Experiments with endotoxin which elevate PMN levels, failed to influence the release of any MAP while elevating albumin levels by greater than 10-fold. Based on these studies, SC release into milk cannot be ascribed to a protease-dependent mechanism.
D000818:Animals; D002417:Cattle; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007118:Immunoassay / Q000379:methods; D007128:Immunoglobulin Fragments / Q000378:metabolism*; D008892:Milk / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D009088:Mucoproteins / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000502:physiology*; D012635:Secretory Component / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013997:Time Factors; D001613:beta 2-Microglobulin / Q000378:metabolism*
D004731:Endotoxins; D007128:Immunoglobulin Fragments; D009088:Mucoproteins; D012635:Secretory Component; D001613:beta 2-Microglobulin; D012710:Serum Albumin, Bovine; D010447:Peptide Hydrolases