Production of auto-anti-idiotypic antibody during the normal immune response. VIII. Effect of auto-anti-idiotypic antibody on contact sensitivity.
An eluate prepared by brief incubation of spleen cells from 2,4,6-trinitrophenyl (TNP) lysyl-Ficoll immunized mice with TNP-epsilon-amino-n-caproic acid causes a specific inhibition of the induction of contact sensitivity by 2,4,6-trinitrochlorobenzene skin painting. The active factor in the eluate binds to an anti-mouse immunoglobulin (Ig) immunoadsorbent column but not to a TNP immunoadsorbent column and is therefore Ig but not anti-TNP antibody. The active factor does bind to an immunoadsorbent prepared from anti-TNP antibody, suggesting that the factor has anti-idiotype specificity. Evidence based upon hapten-reversible inhibition of plaque formation and an enzyme-linked immunosorbent assay (ELISA) indicates that the eluates contain auto-anti-idiotype antibody specific for anti-TNP antibody. It is suggested that auto-anti-idiotype antibody spontaneously produced during the immune response to a T-independent antigen can specifically downregulate contact sensitization to the same epitope.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000917:Antibody Formation; D001323:Autoantibodies / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007163:Immunosorbent Techniques; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014301:Trinitrobenzenes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D011948:Receptors, Antigen, T-Cell; D014301:Trinitrobenzenes; D010081:Oxazolone; C029222:sym-trinitrobenzene