Regulation of functional mRNA levels for trypsin-like esteroproteases by 5 alpha-dihydrotestosterone and triiodothyronine in mouse submandibular salivary gland.
The effects of 5 alpha-dihydrotestosterone and triiodothyronine on the levels of mRNA for trypsin-like esteroproteases in the submandibular glands of young female mice were examined using a translation system in a reticulocyte lysate. Immunoprecipitation of the trypsin-like esteroproteases synthesized in vitro gave almost the same isozyme profile as that of enzymes synthesized in vivo on isoelectric focusing in acrylamide gel containing 8 M urea. Although 5 alpha-dihydrotestosterone and triiodothyronine induced the enzyme activity 3-5 times, these hormones increased their functional mRNA levels only 45-70 per cent. These results suggest that there is little if any post-translational processing of esteroproteases and that hormones increase both the amount of mRNA and the efficacy of its translation.
D000818:Animals; D013196:Dihydrotestosterone / Q000494:pharmacology*; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D007527:Isoenzymes; D051379:Mice; D008813:Mice, Inbred ICR; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*
D007527:Isoenzymes; D012333:RNA, Messenger; D014284:Triiodothyronine; D013196:Dihydrotestosterone; D010450:Endopeptidases