Relevance of hemin for in vitro differentiation of Trypanosoma cruzi.
Simple culture conditions that allow good growth and high yields of trypomastigotes are described. The proportion of metacyclic trypomastigotes increases with the concentration of hemin in the culture medium, reaching a peak of 80% after 10 days with 20 mg hemin/liter.
D000818:Animals; D003470:Culture Media; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D007700:Kinetics; D010274:Parasitology / Q000379:methods; D014349:Trypanosoma cruzi / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
D003470:Culture Media; D006418:Heme; D006427:Hemin