Heterogeneity in the sizes of proteins which diffuse across the outer membrane barrier of bacteria.
Proteins of both small and large molecular sizes diffuse together across the outer membrane barrier of bacterial cells. Once outside, the proteins present in convenient extracts react in agar-gel diffusions against correspondingly antibacterial induced antibodies by precipitation lines that occur at various distances from the wells containing the antigens. The distances between the precipitation lines depend on the velocity with which the proteins are moving in the agar gel; their speed is a result of the molecular sizes of the proteins. The differences involve the intrinsic qualities of the species that synthesize the antigens.
D000936:Antigen-Antibody Complex / Q000032:analysis; D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism; D007106:Immune Sera; D012475:Salmonella / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism; D015008:Yersinia enterocolitica / Q000378:metabolism
D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; D007106:Immune Sera