Monoclonal antibody-dependent murine macrophage-mediated cytotoxicity against human tumors is stimulated by lentinan.
The beta (1----3) glucan lentinan was tested for its capacity to increase the cytotoxic effect of murine peritoneal macrophages for human tumor cells in the presence of monoclonal antibodies (MAbs). Activity was maximum in macrophages obtained on day 5 following intraperitoneal injection of CBA mice with 2.5 mg of lentinan per kg body weight. Macrophages stimulated by lentinan were cytotoxic in conjunction with IgG1, IgG2a and IgG3 MAbs, but not with IgG2b, IgM, or IgA MAbs. The demonstration of a similar enhancing effect by lentinan on human macrophages might have implications for potentiating the tumoricidal effect of these macrophages in the presence of tumor antigen-specific MAbs.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006801:Humans; D007167:Immunotherapy; D007912:Lentinan / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D009369:Neoplasms / Q000473:pathology* / Q000628:therapy; D011134:Polysaccharides / Q000494:pharmacology*; D013864:Thioglycolates / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D011134:Polysaccharides; D013864:Thioglycolates; D007912:Lentinan