Phosphorylated high mannose-type and hybrid-type oligosaccharide chains of human thyroglobulin isolated from malignant thyroid tissue.
Structures of anionic oligosaccharides released by endo-beta-N-acetylglucosaminidase H from human thyroglobulin (19 S) purified from normal and malignant (papillary carcinoma) thyroid tissues were studied by sequential exoglycosidase digestion and lectin affinity chromatography. It was revealed that sialylated hybrid-type oligosaccharides were present in thyroglobulin from normal thyroid tissue, whereas, in the case of thyroglobulin from malignant thyroid tissue, phosphorylated high-mannose type and hybrid-type oligosaccharides each containing one phosphate group in a diester linkage were present instead of sialylated oligosaccharides. The possible meaning of the phosphorylated oligosaccharides was discussed in relation to the low thyroglobulin content in malignant thyroid tissue.
D000118:Acetylglucosaminidase / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000378:metabolism*; D002845:Chromatography; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008358:Mannose / Q000378:metabolism*; D008360:Mannosephosphates / Q000378:metabolism; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D009439:Neuraminidase / Q000378:metabolism; D009844:Oligosaccharides / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D013954:Thyroglobulin / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism*
D008360:Mannosephosphates; D009844:Oligosaccharides; C027693:mannose-6-phosphate; D013954:Thyroglobulin; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008358:Mannose