The effect of interleukin 3 and GM-CSA-2 on megakaryocyte and myeloid clonal colony formation.
Two separate helper T cell-derived lymphokines, interleukin 3 and granulocyte-macrophage colony-stimulating activity-2, were found to stimulate a broad and similar range of hemopoietic colonies in in vitro soft agar cultures including granulocyte, macrophage, granulocyte-macrophage, megakaryocyte, and mixed megakaryocyte colonies. Both lymphokines were potent stimulators of in vitro megakaryocyte colony formation. At plateau levels of IL-3, megakaryocyte colony formation was increased by biologic activity in pokeweed mitogen spleen-conditioned media.
D000818:Animals; D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002999:Clone Cells / Q000502:physiology; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003470:Culture Media; D006098:Granulocytes / Q000502:physiology; D006410:Hematopoiesis; D007377:Interleukin-3; D008222:Lymphokines / Q000494:pharmacology*; D008264:Macrophages / Q000502:physiology; D008533:Megakaryocytes / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR
D003115:Colony-Stimulating Factors; D003470:Culture Media; D007377:Interleukin-3; D008222:Lymphokines