Magnesium enhances human pancreatic elastase digestion of 125I-labeled elastin.
The effect of some divalent cations, especially Mg++, on elastinolysis by porcine or human pancreatic elastase has been determined using 125Iodine-labeled elastin as substrate. Elastin degradation was significantly increased in the presence of 10(-3) M Mg++. If elastin was pre-incubated with 0.5 (w/v) Triton, there was a further increase in elastinolysis to 2.6 times the original rate.
D000818:Animals; D002413:Cations, Divalent; D004492:Edetic Acid / Q000494:pharmacology; D004549:Elastin / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013552:Swine
D002413:Cations, Divalent; D011092:Polyethylene Glycols; D004549:Elastin; D004492:Edetic Acid; D010196:Pancreatic Elastase; D008274:Magnesium