Radioimmunoassay of Pinellia ternata lectin.
Radioimmunoassay of Pinellia ternata lectin (PTL) in rabbit serum and in root extract has been tried. The PTL was labelled with 125I using chloramine-T, and the labelled product was purified by gel chromatography. Standard suppression lines were obtained and the parallelism in serum or buffer was demonstrated. The assay time was 48 h with an intra- and inter-assay coefficient of variation of less than 12%. The PTL content in dried Pinellia ternata roots was about 28 +/- 3 mg/g. More than 40% of the i.v. injected PTL to rabbits was retained in serum at 1 h post administration.
D000818:Animals; D007106:Immune Sera; D007457:Iodine Radioisotopes; D037102:Lectins / Q000032:analysis*; D037121:Plant Lectins; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D011865:Radioisotope Dilution Technique
D007106:Immune Sera; D007457:Iodine Radioisotopes; D037102:Lectins; C047938:Pinellia ternata lectin; D037121:Plant Lectins