[Use of protein A in the immunosorbent analysis of antibodies to the tick-borne encephalitis virus].
The results of the studies made with a view to developing the method for the determination of specific antibodies to the antigen of tick-borne encephalitis virus in human blood serum and liquor are presented. The method is based on the capacity of Staphylococcus aureus protein A to bind with Fc-region of immunoglobulins, which makes it possible to use this protein as the "second" system of antibodies. The conditions for the sorption of the antigen on polystyrene test tubes and for binding 125I-or horse radish peroxidase-labeled protein A preparations with antibodies have been determined, and the method has been approved in tests made on sera and liquor obtained from donors and tick-borne encephalitis patients.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology* / Q000302:isolation & purification; D004675:Encephalitis, Tick-Borne / Q000175:diagnosis / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D051379:Mice; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013205:Staphylococcal Protein A; D014776:Virus Cultivation
D000914:Antibodies, Viral; D000956:Antigens, Viral; D013205:Staphylococcal Protein A
Primenenie belka A v immunosorbentnom analize antitel k virusu kleshchevogo éntsefalita.