Enzyme immunoassay of human protein C by using monoclonal antibodies.
An enzyme-linked immunosorbent assay (ELISA) for measuring human protein C by using two monoclonal antibodies directed toward the heavy chain of protein C is reported. This assay enabled the determination of protein C in concentrations of 10 to 400 ng/ml in less than 3 hours with a single antigen-antibody reaction. Within-run and between-run coefficients of variation were less than 8%. The mean concentrations of protein C in plasma of 42 normal subjects, 24 patients with liver disease, 27 with DIC, 48 with warfarin therapy and 15 with congenital protein C deficiency, were 4.2, 3.0, 2.3, 2.1 and 1.9 micrograms/ml, respectively. The results obtained with the present ELISA correlated well with those of radioimmunoassay (r = 0.935, n = 81) as well as those of Laurell's Rocket method (r = 0.910, n = 81) by using rabbit anti-human protein C serum. The present method was sensitive and specific for measurement of protein C and also PIVKA-protein C in plasma.
D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers; D004211:Disseminated Intravascular Coagulation / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000172:deficiency / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D008107:Liver Diseases / Q000097:blood; D008297:Male; D011486:Protein C; D011498:Protein Precursors / Q000032:analysis; D011516:Prothrombin / Q000032:analysis; D014859:Warfarin / Q000627:therapeutic use
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006023:Glycoproteins; D011486:Protein C; D011498:Protein Precursors; C008208:acarboxyprothrombin; D014859:Warfarin; D011516:Prothrombin