Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents.
When exposed to DNA-damaging agents, the yeast Saccharomyces cerevisiae induces the expression of at least six specific genes. We have previously identified one damage inducible (DIN) gene as a gene fusion (din-lacZ fusion) whose expression increases in response to DNA-damaging treatments. We describe here the identification of five additional DIN genes as din-lacZ fusions and the responses of all six DIN genes to DNA-damaging agents. Northern blot analyses of the transcripts of two of the DIN genes show that their levels increase after exposure to DNA-damaging agents. Five of the din-lacZ fusions are induced in S. cerevisiae cells exposed to UV light, gamma rays, methotrexate, or alkylating agents. One of the din-lacZ fusions is induced by either UV or methotrexate but not by the other agents. This finding suggests that there are sets of DIN genes that are regulated differently.
D000477:Alkylating Agents; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant; D005786:Gene Expression Regulation / Q000187:drug effects*; D005800:Genes, Fungal; D007700:Kinetics; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000235:genetics
D000477:Alkylating Agents; D004271:DNA, Fungal; D004274:DNA, Recombinant; D008741:Methyl Methanesulfonate; D001616:beta-Galactosidase