Determination of glycolic acid level in higher plants during photorespiration by stable isotope dilution mass spectrometry with double-labeling experiments.
Determination of glycolic acid by stable isotope dilution was applied to the measurement of the glycolic acid pool size in tomato and maize leaves during photorespiration. Detached leaves were maintained in the presence of 18O2; [13C]glycolate was added to the foliar extract as an internal standard and the mixture of biological glycolate and [13C]glycolate was analyzed by combined gas chromatography-mass spectrometry. The level of foliar glycolate pool was measured via the 13C label, and 18O incorporation was determined.
D002247:Carbon Isotopes; D008401:Gas Chromatography-Mass Spectrometry; D006016:Glycolates / Q000032:analysis*; D007201:Indicator Dilution Techniques; D007553:Isotope Labeling; D008027:Light; D008433:Mathematics; D010101:Oxygen Consumption; D010103:Oxygen Isotopes; D010944:Plants / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism
D002247:Carbon Isotopes; D006016:Glycolates; D010103:Oxygen Isotopes; C031149:glycolic acid