Bovine neutrophils treated with chemotactic agents: morphologic changes.
Neutrophils were isolated from the peripheral blood of cattle. After the neutrophils were incubated with zymosan-activated serum, the neutrophils changed from spherical to a bipolar shape. Ninety percent of the neutrophils became bipolar in 5 to 10 minutes. Bacterial cell filtrate and casein also induced bipolar shape changes in neutrophils, but N-formyl-L-methionyl-L-leucinyl-L-phenylalanine did not. The neutrophil-shape-change response was a rapid in vitro assay to evaluate early chemotactic events.
D000818:Animals; D002364:Caseins / Q000494:pharmacology*; D002417:Cattle / Q000097:blood*; D003470:Culture Media / Q000494:pharmacology*; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects; D004926:Escherichia coli; D005260:Female; D007774:Lactation; D008858:Microscopy, Phase-Contrast; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D011247:Pregnancy
D002364:Caseins; D003470:Culture Media; D009240:N-Formylmethionine Leucyl-Phenylalanine