Immunocytochemical localization of the cytochrome b/f complex of chloroplast thylakoid membranes.
Antibodies directed against purified cytochrome f, isolated from the cytochrome b/f complex of spinach chloroplasts, were used in on-grid immunogold labelling studies of spinach leaf tissue. Our results show unambiguously that cytochrome f, and hence the cytochrome b/f complex, is located in both appressed and non-appressed thylakoid membranes.
D002736:Chloroplasts / Q000201:enzymology* / Q000648:ultrastructure; D003573:Cytochrome b Group / Q000276:immunology / Q000378:metabolism*; D003580:Cytochromes / Q000276:immunology / Q000378:metabolism*; D045348:Cytochromes f; D006046:Gold; D007158:Immunologic Techniques; D007425:Intracellular Membranes / Q000201:enzymology; D008854:Microscopy, Electron; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology / Q000648:ultrastructure
D003573:Cytochrome b Group; D003580:Cytochromes; D006046:Gold; D045348:Cytochromes f