The use of alkaline-phosphatase conjugated second antibody for the enhancement of immunoprecipitation techniques on cellulose acetate membranes.
A technique is described using alkaline phosphatase (ALP) conjugated second antibodies on cellulose acetate membrane (CAM) for enhancement of immunoprecipitation techniques. The method is extremely sensitive, detecting as little as 0.1 mg/l of protein and has particular application in the investigation of monoclonal immunoglobulins in human serum or urine and in hybridoma supernatants or ascitic fluids.
D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001536:Bence Jones Protein / Q000652:urine; D001798:Blood Proteins / Q000032:analysis; D002482:Cellulose / Q000031:analogs & derivatives; D006801:Humans; D006825:Hybridomas / Q000032:analysis; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007136:Immunoglobulins / Q000032:analysis*; D007163:Immunosorbent Techniques; D008722:Methods; D051379:Mice; D011817:Rabbits
D000906:Antibodies; D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D007136:Immunoglobulins; C005062:acetylcellulose; D002482:Cellulose; D001536:Bence Jones Protein; D000469:Alkaline Phosphatase