Immobilization of DNA via covalent linkage for use as immunosorbent.
DNA was immobilized covalently to Sepharose by several methods using epichlorohydrin, cyanogen bromide, carbodiimide, hydroxysuccinimide, carbonyldiimidazole, trichlorotriazine, and diazonium salt. These immobilizing methods were compared from the standpoint of the preparation of immunosorbent for anti-DNA antibodies. Among these methods, that involving epichlorohydrin was the most suitable because of large coupling capacity, stability of bound DNA, and nonadsorption of anti-DNA by the support itself.
D000818:Animals; D002021:Buffers; D003488:Cyanogen Bromide; D004247:DNA / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D008024:Ligands; D013696:Temperature; D013997:Time Factors
D002021:Buffers; D007164:Immunosorbents; D008024:Ligands; D004247:DNA; D003488:Cyanogen Bromide