Preparation and characterization of monoclonal antibodies to the trichothecene mycotoxin T-2.
Two mouse immunoglobulin G1 monoclonal antibodies that bind to the trichothecene mycotoxin T-2 were prepared. These antibodies, designated 12C12 and 15H6, had affinities for T-2 of 3.5 X 10(6) and 5.8 X 10(7) liters/mol, respectively. A competitive inhibition enzyme immunoassay that employed these antibodies had a sensitivity for T-2 of 50 ng per assay. Both antibodies bound to the metabolite HT-2 but not to the related trichothecenes monoacetoxyscirpenol, diacetoxyscirpenol, deoxynivalenol, and deoxyverrucarol. Evidence is presented that T-2-protein conjugates inhibit protein synthesis in lymphoid cells and that this apparent immunotoxicity may be due to the release of T-2 from the protein carrier.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D005260:Female; D006825:Hybridomas; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D012717:Sesquiterpenes / Q000276:immunology*; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012717:Sesquiterpenes; D013605:T-2 Toxin; C012351:HT-2 toxin