Progress in temporal bone histopathology. II. Immuno-technology applied to the temporal bone.
By using monoclonal antibodies against cell-membrane receptors the cellular composition of an aural cholesteatoma can be determined. Langerhans' cells and T-lymphocytes predominate in the cholesteatoma matrix. The normal human tympanic membrane is devoid of these cells. Immuno-technology applied to the inner ear is illustrated by tracing gentamicin in the cochlea with a polyvalent anti-gentamicin antiserum in serial sections. In an animal model gentamicin is detectable through this method. From 3 days on, after daily i.p. injection of 100 mg gentamicin/kg body weight, the drug accumulates in the outer hair cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D002781:Cholesteatoma / Q000473:pathology*; D004427:Ear Diseases / Q000473:pathology*; D005839:Gentamicins / Q000032:analysis; D006198:Hair Cells, Auditory / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007801:Langerhans Cells / Q000473:pathology; D011817:Rabbits; D013601:T-Lymphocytes / Q000473:pathology; D013701:Temporal Bone / Q000473:pathology*; D014432:Tympanic Membrane / Q000473:pathology
D000911:Antibodies, Monoclonal; D005839:Gentamicins