Interaction of human T lymphocytes with E. coli lipopolysaccharide (LPS).
We have examined the interaction of fluorescein isothiocyanate (FITC) labeled E. coli lipopolysaccharide (LPS) with human T lymphocytes by means of cell flow cytometry, in an effort to establish the type and the conditions of this interaction. Our results indicate that the interaction of LPS with human T lymphocytes is optimal at 37 degrees C. This interaction is rapid and reaches the maximum after 5 minutes and is followed by endocytosis. Our results show that sodium azide and trypsin treatment does not affect LPS- human T cell interaction, suggesting a nonspecific lipid-lipid interaction.
D004926:Escherichia coli; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D008070:Lipopolysaccharides