Regulation of nitrogen fixation in Rhodospirillum rubrum grown under dark, fermentative conditions.
Rhodospirillum rubrum was shown to grow fermentatively on fructose with N2 as a nitrogen source. The nitrogenase activity of these cells was regulated by the NH4+ switch-off/switch-on mechanism in a manner identical to that for photosynthetically grown cells. In vitro, the inactive nitrogenase Fe protein from fermenting cells was reactivated by an endogenous membrane-bound, Mn2+-dependent activating enzyme that was interchangeable with the activating enzyme isolated from photosynthetic membranes.
D003624:Darkness; D005285:Fermentation; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012247:Rhodospirillum rubrum / Q000378:metabolism*
D000644:Quaternary Ammonium Compounds; D009591:Nitrogenase