Fibroblast products adsorb to the culture substrate and stimulate growth of human umbilical vein endothelial cells.
Human umbilical vein endothelial cells grew with a doubling time of approximately 20 hr in medium conditioned by human diploid fibroblasts and supplemented with 10% fetal bovine serum, whereas the cells did not grow substantially in the non-conditioned serum supplemented medium. Production of the fibroblast-derived activity required the presence of insulin, EGF, or PDGF. The fibroblast derived-factor adsorbed to native culture dishes or dishes coated with gelatin and collagen. The adsorbed activity was resistant to treatment with 1% Triton X-100, and was abolished by treatment with serine proteases. Further, the extracellular matrix produced by the fibroblasts also showed growth-stimulating activity. Fibroblast-derived factors may play a role in vascularization processes during wound healing, inflammation and normal development.
D000327:Adsorption; D002455:Cell Division; D003094:Collagen; D003470:Culture Media; D010450:Endopeptidases / Q000378:metabolism; D004727:Endothelium / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D005780:Gelatin; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D017830:Octoxynol; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011247:Pregnancy; D012697:Serine Endopeptidases; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology
D003470:Culture Media; D007328:Insulin; D010982:Platelet-Derived Growth Factor; D011092:Polyethylene Glycols; D004815:Epidermal Growth Factor; D005780:Gelatin; D017830:Octoxynol; D003094:Collagen; D010450:Endopeptidases; D012697:Serine Endopeptidases