Antitermination is required for readthrough transcription of the maize rbcL gene by a bacteriophage promoter in Escherichia coli.
Sequences upstream of the 5' end of the rbcL gene in maize chloroplast fragment Bam 9, have a polar effect on the expression of rbcL from an external upstream bacteriophage PL promoter in Escherichia coli. This polarity can be suppressed by the bacteriophage transcription antitermination protein N or Q. The requirement for transcription antitermination is abolished if DNA upstream of rbcL is removed by a deletion. We have also investigated the ability of RNA polymerase initiating transcription at PL in the presence of N to transcribe through the normal rbcL transcription terminator and into sequences beyond. RNA polymerase initiating at PL can traverse rbcL and its 5' and 3'-flanking regions in the presence of N.
D001483:Base Sequence; D002736:Chloroplasts; D003090:Coliphages / Q000235:genetics; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics*
D004247:DNA; D012273:Ribulose-Bisphosphate Carboxylase