Evaluation of the Spot-CAMP test for the rapid presumptive identification of group B streptococci.
A rapid Spot-CAMP test was evaluated for its ability to accurately identify colonies of Streptococcus agalactiae (Lancefield Group B) growing on primary sheep blood agar plates. The test uses a beta-lysin-containing filtrate, which is prepared from a broth culture of Staphylococcus aureus. A drop of beta-lysin filtrate is applied adjacent to a suspected group B Streptococcus (GBS) colony and the plate is incubated and then examined for a zone of synergistic hemolysis. The Spot-CAMP test demonstrated 100% correlation with both a Standard CAMP procedure and Lancefield serogrouping. The rapid Spot-CAMP test was easy to perform and inexpensive, and could presumptively identify within 30 minutes colonies of GBS growing on primary isolation plates.
D023181:Antimicrobial Cationic Peptides; D001427:Bacterial Toxins; D001431:Bacteriological Techniques; D001798:Blood Proteins; D006460:Hemolysin Proteins; D006801:Humans; D011506:Proteins; D013108:Sphingomyelin Phosphodiesterase; D013290:Streptococcal Infections / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000302:isolation & purification*
D023181:Antimicrobial Cationic Peptides; D001427:Bacterial Toxins; D001798:Blood Proteins; D006460:Hemolysin Proteins; D011506:Proteins; C011679:beta lysin, human; D013108:Sphingomyelin Phosphodiesterase; C029878:hlb protein, Staphylococcus aureus