The amino acid sequence of thiogalactoside transacetylase of Escherichia coli.
The amino acid sequence of thiogalactoside transacetylase, a dimer, has been determined. The monomer contains 202 amino acid residues in a single polypeptide chain and has a molecular weight of 22,671. The analysis was carried out by treatment of the carboxymethylated protein with cyanogen bromide and with trypsin. All seven cyanogen bromide peptides were isolated in pure form and were ordered by peptides isolated from tryptic digests. The sequence analysis was aided by determination of the DNA sequence of the lacA gene. The amino terminus of the protein is heterogenous because the initiator methionine is only partially cleaved. Another rather unusual feature of this cytoplasmic protein is a very hydrophobic segment in the center portion of the chain. Comparison of the amino acid sequence of thiogalactoside transacetylase to those of the lac repressor, beta-galactosidase, and lactose permease did not reveal any marked similarities. Therefore, there is no obvious evolutionary relatedness among proteins of the Lactose Operon.
D000123:Acetyltransferases / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D007763:Lac Operon; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments / Q000302:isolation & purification; D027981:Symporters; D014357:Trypsin; D001616:beta-Galactosidase
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments; D027981:Symporters; C021499:lactose permease; D000123:Acetyltransferases; C022770:galactoside acetyltransferase; D001616:beta-Galactosidase; D014357:Trypsin; D003488:Cyanogen Bromide