Acidic acrosin inhibitors from bull seminal plasma. Structural differences.
The three acidic acrosin inhibitors of bull seminal plasma, BUSI I A, BUSI I B1 and BUSI I B2 were compared by thin-layer chromatographic and high-performance liquid chromatographic fingerprint analyses of the tryptic digests prepared from their S-carboxymethylated derivatives. It was found that the inhibitors differ only in their N-terminal regions. The inhibitor BUSI I B1 has a blocked N-terminus due to a pyroglutamic-acid residue. This residue is substituted by glutamic acid in BUSI I B2. The third inhibitor, BUSI I A, is four residues shorter at the N-terminus than the two other inhibitors. A high-performance liquid chromatography-based method for the separation of the three inhibitor variants was developed.
D000176:Acrosin / Q000037:antagonists & inhibitors*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003488:Cyanogen Bromide; D066298:In Vitro Techniques; D008297:Male; D010455:Peptides / Q000032:analysis; D011480:Protease Inhibitors; D012661:Semen / Q000032:analysis*; D014357:Trypsin
D000596:Amino Acids; D010455:Peptides; D011480:Protease Inhibitors; D000176:Acrosin; D014357:Trypsin; D003488:Cyanogen Bromide