Immunogold labeling of organelles in the bioluminescent dinoflagellate Gonyaulax polyedra with anti-luciferase antibody.
A polyclonal antibody directed against the luciferase of the luminous dinoflagellate Gonyaulax polyedra labels both dense vesicles and trichocyst sheaths, as visualized in the electron microscope after treatment of antibody-reacted sections with an immunogold probe. Because of their similar size, shape and localization, the dense vesicles seen with the electron microscope are postulated to correspond to autofluorescent particles seen with the fluorescent microscope, which are known to be the origin of bioluminescent flashes in this alga. The explanation for the trichocyst sheath-specific labeling is less evident. The possibility that a second antibody of different specificity is involved has not been excluded but seems unlikely. Alternatively, it could be due to a different but antigenically cross-reacting protein. But the possibility that luciferase itself occurs in two different organelles is intriguing and consistent with previous biochemical studies of cell extracts.
D000818:Animals; D003594:Cytoplasmic Granules / Q000201:enzymology; D004141:Dinoflagellida / Q000201:enzymology* / Q000648:ultrastructure; D006046:Gold; D007158:Immunologic Techniques; D008156:Luciferases / Q000276:immunology / Q000378:metabolism*; D008163:Luminescent Measurements; D009940:Organoids / Q000201:enzymology
D006046:Gold; D008156:Luciferases