Evidence that a system similar to the recA system of Escherichia coli exists in Vibrio cholerae.
Two lines of evidence suggest that a gene analogous to the recA gene of Escherichia coli exists in Vibrio cholerae and that its product serves a proteolytic function in the SOS response. Firstly, Southern blot hybridization using the recA gene of E. coli as a probe revealed a genomic sequence in V. cholerae which hybridized with the probe. Secondly, the SOS-like response in V. cholerae (as measured by beta phage induction) triggered by DNA damaging agents like Furazolidone could be blocked by Antipain, a protease inhibitor known to inhibit RecA protease action in E. coli. Maximal blocking effect of Antipain on beta phage induction occurred at 1 mM. At this concentration neither the viability of the host bacterium nor the lytic growth of a clear plaque mutant of the phage was affected by Antipain.
D000976:Antipain / Q000494:pharmacology; D001435:Bacteriophages / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011938:Rec A Recombinases / Q000235:genetics* / Q000302:isolation & purification; D014734:Vibrio cholerae / Q000235:genetics*
D000976:Antipain; D011938:Rec A Recombinases