Enzyme-linked immunosorbent assay for determination of anti-mitochondrial antibodies.
An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of human auto antibodies to mitochondria (AMA). The ELISA was compared to the previous routine method - indirect immunofluorescence technique (IIF)--and optimized for the specific detection of patients with primary biliary cirrhosis. Sera from 72 patients with primary biliary cirrhosis, 10 sera positive for anti-cardiolipin antibodies from patients with syphilis, 9 patients with drug-induced pseudolupus erythematosus, 19 patients with non-alcoholic chronic active hepatitis, 14 patients with systemic lupus erythematosus, 20 patients with rheumatoid arthritis and 100 healthy blood-donors were examined for AMA by both methods. The nosological sensitivity for the ELISA method was comparable to the IIF. The ELISA method was accurate, precise, inexpensive and well-suited as a diagnostic screening method for AMA when primary biliary cirrhosis is suspected. Furthermore, ELISA methods require less experience of the observer than IIF.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001327:Autoimmune Diseases; D002308:Cardiolipins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D005455:Fluorescent Antibody Technique; D006521:Hepatitis, Chronic / Q000175:diagnosis; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000175:diagnosis; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis; D008929:Mitochondria, Heart / Q000276:immunology*; D013552:Swine
D000906:Antibodies; D002308:Cardiolipins