Lipoxygenase specific inhibitors inhibit murine lymphocyte reactivity to Con A by reducing IL-2 production and its action.
Caffeic acid and AA861, specific inhibitors of lipoxygenase, reduced reactivity of murine spleen lymphocytes to Concanavalin A (Con A). They inhibited interleukin-2 (IL-2) production and its action, although they did not inhibit acquisition of lymphocyte reactivity to IL-2. Indomethacin was not able to restore the reactivity of lymphocytes to Con A even when it was combined with lipoxygenase specific inhibitors. This result suggests that lipoxygenase inhibitors exert their effects not through increasing prostaglandin E2 (PGE2) production but through inhibiting lipoxygenase. Lipoxygenase inhibitors exerted their effects even though they were removed 24 hr after the onset of culture. Taken together, these results suggest that lipoxygenase inhibitors exerted their inhibitory effects in the early period of lymphocyte activation by reducing IL-2 production and its action, implying that lipoxygenase reactions during 24 hr after stimulation may be involved in these steps.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000818:Animals; D016227:Benzoquinones; D002109:Caffeic Acids / Q000494:pharmacology*; D002478:Cells, Cultured; D002934:Cinnamates / Q000494:pharmacology*; D003208:Concanavalin A / Q000037:antagonists & inhibitors*; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis*; D016859:Lipoxygenase Inhibitors; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011809:Quinones / Q000494:pharmacology*
D016227:Benzoquinones; D002109:Caffeic Acids; D002934:Cinnamates; D007376:Interleukin-2; D016859:Lipoxygenase Inhibitors; D011809:Quinones; D003208:Concanavalin A; D015117:5,8,11,14-Eicosatetraynoic Acid; C036837:2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone; C040048:caffeic acid; D007213:Indomethacin