Stromal cells (CFU-F) in normal and genetically anemic mouse strains.
Mice of the Sl/Sld genotype have an approximately 3-fold higher number of fibroblastoid progenitors (CFU-F) in their spleens than their normal +/+ littermates. Experiments were performed to determine whether the elevated Sl/Sld splenic CFU-F numbers were due to compensatory mechanisms acting in the presence of a functionally abnormal CFU-F population or to a nonspecific response to chronic anemia. Comparison of the functional ability of Sl/Sld splenic fibroblasts to produce granulocyte/macrophage colony-stimulating activity with +/+ splenic fibroblasts demonstrated that there was no difference. Similar results were obtained for Sl/Sld and +/+ femoral fibroblasts. Analysis of CFU-F in W/Wv mice revealed an approximately 3-fold higher number of splenic CFU-F than in either +/+ or heterozygous (W/+ and Wv/+) littermates. Since the anemia in W/Wv mice is attributed to a hemopoietic stem cell defect and that of the Sl/Sld mice is attributed to a microenvironmental defect, we suggest that the increased splenic CFU-F number in Sl/Sld mice is not specifically due to the microenvironmental defect, but is part of a general response to hemopoietic stress.
D000748:Anemia, Macrocytic / Q000097:blood / Q000235:genetics / Q000473:pathology*; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology*; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006579:Heterozygote; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains
D003115:Colony-Stimulating Factors