Effect of hypertonicity on the increase in basolateral conductance of Necturus small intestine in response to Na+-sugar cotransport.
Exposure of Necturus small intestine to a galactose-containing perfusate that is 20% hypertonic compared to the galactose-free (control) perfusate results in a rapid depolarization of the electrical potential difference across the apical membrane, psi mc, and a decrease in the ratio of the resistance of the apical membrane to that of the basolateral membrane, (rm/rs); however, the slow repolarization of psi mc and increase in (rm/rs), observed under isotonic conditions, is blocked. These findings are consistent with the notion that the increase in the conductance of the basolateral membrane in response to Na+-coupled sugar (or amino acid) transport across the apical membrane may be a 'volume regulatory response' to cell swelling.
D000818:Animals; D001693:Biological Transport, Active; D050260:Carbohydrate Metabolism; D004553:Electric Conductivity; D004594:Electrophysiology; D005690:Galactose / Q000378:metabolism; D007421:Intestine, Small / Q000502:physiology*; D009337:Necturus; D009994:Osmolar Concentration; D010477:Perfusion; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
D012964:Sodium; D011188:Potassium; D005690:Galactose