title: Chromatin structure of genes during the cell cycle.
abstract: K562 cells were separated by centrifugal elutriation into 5 fractions each of which had a unique distribution of cells at different phases of the cell cycle. A study of DNAseI sensitivity of the epsilon- and gamma-globin genes, which are expressed in these cells, and the lambda light chain immunoglobulin gene, which is not expressed, in nuclei from each of the 5 fractions was undertaken. There was no detectable difference in the arrangement of DNAseI-hypersensitive sites (DHS) in chromatin of each of the three genes in the different fractions.
mesh_terms: D002453:Cell Cycle; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism*; D002999:Clone Cells / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000494:pharmacology; D005796:Genes; D005914:Globins / Q000235:genetics; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D007146:Immunoglobulin lambda-Chains; D005914:Globins; D004247:DNA; D003850:Deoxyribonuclease I