Survival of intracerebrally grafted rat dopamine neurons previously cultured in vitro.
Fetal rat dopamine (DA) neurons were cultured in vitro for a 6-day period and transported, after redissociation, for 2 days prior to being grafted to the neostriatum of adult rats with 6-hydroxydopamine lesions of the ascending nigrostriatal pathway. In 2 of the 5 graft recipients that were tested for amphetamine-induced motor asymmetry, the grafts eliminated the lesion-induced turning behaviour within 3-6 weeks after transplantation. Fluorescence histochemistry revealed surviving grafts in all 6 recipients at 7 weeks after transplantation, containing between 42 and 125 DA neurons. The number of surviving DA neurons in the 3 non-compensated rats was below the minimum number of cells previously found to be necessary for functional effects on turning behaviour to occur.
D000818:Animals; D001927:Brain Diseases / Q000139:chemically induced / Q000628:therapy; D002478:Cells, Cultured; D003342:Corpus Striatum; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D005333:Fetus; D006892:Hydroxydopamines; D008636:Mesencephalon / Q000637:transplantation*; D009434:Neural Pathways; D016627:Oxidopamine; D051381:Rats; D011919:Rats, Inbred Strains; D013378:Substantia Nigra
D006892:Hydroxydopamines; D016627:Oxidopamine; D004298:Dopamine