Control of STA1 gene expression by the mating-type locus in yeasts.
Extracellular production of glucoamylase from the yeast Saccharomyces diastaticus carrying an STA1 gene (a structural gene for the enzyme) is repressed by the mating-type constitution a/alpha. We obtained mutants that were relieved from the mating-type control of the STA1 gene expression. Tetrad analysis showed that the mutation was closely linked to MATa. The mutation was complemented by a MATa2 gene; hence, it was designated mata2. Northern blot analysis revealed that the MATa2-mediated repression occurs at the posttranscriptional level.
D004794:Enzyme Repression; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000235:genetics*; D005959:Glucosidases / Q000235:genetics*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic
D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase