A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human red blood cells.
A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53 degrees-55 degrees C for 15 min. The method is simple and highly efficient with a fair recovery of 15.6%-34.4%.
D000017:ABO Blood-Group System / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D004912:Erythrocytes / Q000276:immunology*; D005404:Fixatives; D005976:Glutaral; D006801:Humans; D007518:Isoantibodies / Q000302:isolation & purification*
D000017:ABO Blood-Group System; D005404:Fixatives; D007518:Isoantibodies; D005976:Glutaral