Phosphorylase kinase isozymes in normal and electrically stimulated skeletal muscles.
Phosphorylase kinase was quantitatively immunoprecipitated from extracts of different rabbit skeletal muscles, subjected to electrophoresis on polyacrylamide gels in the presence of sodium dodecyl sulfate, and stained with silver. Amounts of the two isozymes, enzymes r and w. were determined by the staining intensities of the alpha'- and alpha -subunits, respectively. The kinase in muscles composed primarily of slow oxidative fibers was almost entirely enzyme r, but several times more of this isozyme was found in muscles with a high proportion of fast oxidative-glycolytic fibers. Enzyme w predominated in muscles composed mostly of fast glycolytic fibers. To investigate the possible role of muscle activity in controlling isozyme levels, tibialis anterior muscles were activated by chronic electrical stimulation of the peroneal nerves. After 10 wk of continuous stimulation, the amount of phosphorylase kinase was decreased by approximately 80%, and the ratio of enzyme r to enzyme w was doubled. These results demonstrate that increased muscle activity can decrease levels of phosphorylase kinase and suggest that activity may regulate the expression of the isozymes in different muscle fiber types.
D000818:Animals; D003720:Densitometry; D004558:Electric Stimulation; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000032:analysis*; D046911:Macromolecular Substances; D009132:Muscles / Q000201:enzymology*; D010764:Phosphorylase Kinase / Q000032:analysis*; D011817:Rabbits; D012834:Silver; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors
D007527:Isoenzymes; D046911:Macromolecular Substances; D012967:Sodium Dodecyl Sulfate; D012834:Silver; D010764:Phosphorylase Kinase