title: High-affinity hemicholinium-3 binding to brain membranes: reduction by treatment with physostigmine in vivo.
abstract: We examined high-affinity, sodium-dependent binding of hemicholinium-3 to brain membranes. Binding, with Kd of about 3 nM, was highest in corpus striatum, intermediate in cerebral cortex and hippocampus, and low in cerebellum. Treatment with physostigmine in vivo reduced binding in all regions without affecting apparent affinity.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D006426:Hemicholinium 3 / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D008566:Membranes / Q000378:metabolism; D010830:Physostigmine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006426:Hemicholinium 3; D010830:Physostigmine