Organic solvent-soluble lipofuscin pigments and glutathione peroxidase in mouse brain and heart: effects of age and vitamin E.
The effect of age and dietary supplementation of vitamin E or N,N'-diphenyl-p-phenylenediamine (DPPD) on organic solvent-soluble lipofuscin pigments (OLP) and glutathione peroxidase (GSH-Px) activity in mouse heart and brain was investigated. Four groups of 32 female weanling mice were fed a basal diet containing either 0, 30 or 300 ppm RRR-alpha-tocopheryl acetate (d-alpha-tocopheryl acetate) or 30 ppm DPPD from 2 to 18 mo of age. Neither GSH-Px activity nor dietary supplementation of vitamin E or DPPD had an effect on OLP concentrations in the brain or heart. OLP levels were two- to fourfold higher at 12 mo of age in the heart and were lower at 18 mo of age in the brain than at 2 or 9 mo of age. GSH-Px activity increased with age in the heart tissue of vitamin E-deficient and DPPD-supplemented mice only. No change in GSH-Px activity was observed in the brain due to diet or increasing age. These results suggested that OLP concentrations were not affected by dietary supplementation of vitamin E or DPPD but were affected by age-related factors in the mouse brain and heart.
D000375:Aging; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001923:Brain Chemistry; D004032:Diet; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism*; D006321:Heart / Q000187:drug effects*; D008062:Lipofuscin / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000032:analysis / Q000378:metabolism*; D010655:Phenylenediamines / Q000494:pharmacology; D010860:Pigments, Biological / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
D008062:Lipofuscin; D010655:Phenylenediamines; D010860:Pigments, Biological; D014810:Vitamin E; C004845:N,N'-diphenyl-4-phenylenediamine; D005979:Glutathione Peroxidase