Combination therapy of Schistosoma japonicum by tubercidin and nitrobenzylthioinosine 5'-monophosphate.
Coadministration of nitrobenzylthioinosine 5'-monophosphate (NBMPR-P) with high doses of tubercidin by i.p. injection into Schistosoma japonicum infected mice beginning 5 weeks post-infection was highly toxic to the parasite but not the hose. Combination therapy resulted in a striking reduction in the number of worms, and the few worms that could be found were stunted. Combination therapy also caused a drastic reduction in the number of eggs in the livers (from 86,500 to 2,800 eggs/liver) and intestines (from 2,200 to 74 eggs/cm2), and 95% of eggs that were found were dead, indicating the termination of oviposition. Mice receiving the combination of tubercidin plus NBMPR-P appeared healthy and had normal size livers and spleens. These results demonstrate that by combining NBMPR-P with tubercidin high selective toxicity against S. japonicum can be achieved, as was shown previously with S. mansoni.
D000818:Animals; D004359:Drug Therapy, Combination; D005260:Female; D007288:Inosine / Q000031:analogs & derivatives*; D007668:Kidney / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D012263:Ribonucleosides / Q000008:administration & dosage*; D012554:Schistosomiasis japonica / Q000188:drug therapy*; D013868:Thioinosine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D013873:Thionucleotides / Q000008:administration & dosage* / Q000633:toxicity; D014372:Tubercidin / Q000008:administration & dosage* / Q000633:toxicity
D012263:Ribonucleosides; D013873:Thionucleotides; D013868:Thioinosine; D007288:Inosine; C028318:nitrobenzylthioinosine 5'-monophosphate; D014372:Tubercidin