title: Isolation and characterization of allergens of Prosopis juliflora pollen grains.
abstract: Proteins and glycoproteins from Prosopis juliflora (Pj) pollen grains were separated by gel filtration, electrophoresis, DEAE cellulose chromatography and their molecular weight was determined by gel filtration and SDS-Polyacrylamide gel electrophoresis. The allergenic activity of different fractions were evaluated by in vivo skin prick test and in vitro gel diffusion test. It was found that fraction E of gel filtration and fraction III and IV of DEAE cellulose chromatography were most allergenic. This fraction E of gel filtration showed positive reaction with periodic acid Schiff's reagent as determined by SDS-gel electrophoresis.
mesh_terms: D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006168:Guinea Pigs; D005779:Immunodiffusion; D008297:Male; D008970:Molecular Weight; D010940:Plant Proteins / Q000302:isolation & purification*; D011058:Pollen / Q000032:analysis*; D012882:Skin Tests
chemical_list: D000485:Allergens; D006023:Glycoproteins; D010940:Plant Proteins