title: A bioassay for determining biotinidase activity and for discriminating biocytin from biotin using holocarboxylase synthetase-deficient cultured fibroblasts.
mesh_terms: D000581:Amidohydrolases / Q000032:analysis* / Q000172:deficiency; D001681:Biological Assay; D001710:Biotin / Q000378:metabolism*; D043523:Biotinidase; D019731:Carbon-Nitrogen Ligases; D002262:Carboxy-Lyases / Q000032:analysis; D002478:Cells, Cultured; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008025:Ligases / Q000172:deficiency*; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D043586:Methylmalonyl-CoA Decarboxylase
chemical_list: D001710:Biotin; D000581:Amidohydrolases; D043523:Biotinidase; D002262:Carboxy-Lyases; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C023312:holocarboxylase synthetases; D043586:Methylmalonyl-CoA Decarboxylase; C013411:biocytin; D008239:Lysine