Analysis of allergen components in grass pollen extracts using immunoblotting.
Sera of 60 allergic persons and 20 healthy RAST-negative control persons were examined using immunoblotting to establish the presence of grass pollen-specific antibodies. These antibodies were depicted by an enzymatic, immunoglobulin class-specific indicator system. Seven bands of the timothy pollen extract proved to be major allergens. One of the 4 grass pollen-specific monoclonal antibodies tested (Bo 1) recognized the 7 major allergen bands. The possibility of employing monoclonal antibodies for the isolation of allergens is discussed.
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D003101:Collodion; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D010209:Paper; D010936:Plant Extracts / Q000276:immunology*; D011058:Pollen / Q000032:analysis*; D011485:Protein Binding
D000906:Antibodies; D000911:Antibodies, Monoclonal; D010936:Plant Extracts; D003101:Collodion