The effect of dietary nitrite and nitrate on the metabolism of sulphamethazine in the rat.
Rats given 100 p.p.m. of 14C-sulphamethazine [4-amino-N-(4,6-dimethyl-2-pyrimidinyl)benzene[U-14C]sulphonamide] in the diet excreted less 14C-activity in the urine as the amount of nitrite (0 to 1000 p.p.m.), but not nitrate (3730 p.p.m.), in the diet was increased. As the level of nitrite, but not nitrate, was increased, there was a concomitant increase in the amount of 14C-desaminosulphamethazine (4-[(N-4,6-dimethyl-2-pyrimidinyl)benzene-[U-14C]-sulphonamide in the blood, liver, skeletal muscle and gastrointestinal tract. As the level of nitrite, but not nitrate, supplementation was increased, the amount of methanol-insoluble 14C-activity in the gastrointestinal tract increased but the amount of insoluble 14C-activity in the blood, skeletal muscle and liver was not changed.
D000818:Animals; D004032:Diet; D008297:Male; D009566:Nitrates / Q000008:administration & dosage / Q000494:pharmacology*; D009573:Nitrites / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013418:Sulfamethazine / Q000378:metabolism*; D014018:Tissue Distribution
D009566:Nitrates; D009573:Nitrites; D013418:Sulfamethazine