Rubidium transport in X-irradiated human erythrocytes.
Outflow of 86Rb, a radioactive analogue of potassium, from human erythrocytes X-irradiated in vitro was studied with the following results. (1) The 86Rb level in the supernatants of irradiated and control cell suspensions reflected mainly 86Rb outflow and much less its active re-uptake. (2) The effect of irradiation on 86Rb outflow was more pronounced at a low temperature (4 degrees C) than at 37 degrees C; the lowest dose of X-radiation exhibiting a significant effect on 86Rb outflow at 4 degrees C was 2.5 Gy. (3) K/Rb exchange did not seem to play an appreciable role in radiation-induced 86Rb outflow. (4) Calcium and its accumulation in irradiated cells was not found to be the cause of the effect of radiation on 86Rb outflow. (5) The effect of radiation on 86Rb outflow was higher in low Na medium but it was not inhibited by bumetanide. Rb/Na counter- or co-transport do not therefore seem to be involved in radiation-induced Rb+ outflow.
D001692:Biological Transport / Q000528:radiation effects; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000097:blood / Q000494:pharmacology; D012413:Rubidium / Q000097:blood*; D012964:Sodium / Q000097:blood; D013696:Temperature
D000001:Calcimycin; D010042:Ouabain; D012964:Sodium; D012413:Rubidium; D011188:Potassium; D002118:Calcium