Effect of pH on the induction of competence and progression to the S-phase in mouse fibroblasts.
The pH dependence of competence induction and progression to the S-phase in quiescent stimulated cells has been studied. The results show that: (i) induction of competence by fibroblast growth factor in these cells is relatively independent of the external pH between pH 5.6-7.6; (ii) progression of cells to the S-phase is highly sensitive to pH and shows a dramatic increase between pH 6.8-7.2. These observations suggest that the intracellular alkalinization triggered by growth factors is fundamental for progression but not for competence induction.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007399:Interphase; D051379:Mice
D005346:Fibroblast Growth Factors; D004247:DNA