title: DNA-damaging activity of tripelennamine in primary cultures of human hepatocytes.
abstract: The genotoxicity of tripelennamine, an antihistamine used in the treatment of allergic disorders, was examined in human hepatocyte primary cultures derived from 3 different donors, after exposure to non-toxic concentrations ranging from 10 to 100 microM. A modest but statistically significant and dose-related amount of autoradiographic DNA repair was present in cultures from two donors. DNA fragmentation, as measured by alkaline elution, was found to occur in dose-dependent amounts in cultures of all the 3 donors. These findings, which agree with the previously observed capability of tripelennamine to induce DNA repair and fragmentation in rat hepatocytes, strengthen the suspicion of a potential genotoxic risk of this drug to humans.
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D005260:Female; D006801:Humans; D008099:Liver / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D014309:Tripelennamine / Q000494:pharmacology*
chemical_list: D014309:Tripelennamine; D004247:DNA