Conjugal transfer of plasmid-mediated antibiotic resistance from streptococci to Clostridium acetobutylicum.
Three broad host-range MLS plasmids, pAM beta 1 (Em), pIP501 (Em, Cm) and pJH4 (Em, Sm, Km), were transferred by a conjugation-like process from Streptococcus faecalis to Clostridium acetobutylicum. The plasmids were stably maintained and antibiotic resistances were fully expressed in C. acetobutylicum, except for chloramphenicol resistance, which was poorly expressed, if at all. The C. acetobutylicum strains harbouring every plasmid could be used as donors in intraspecific matings with frequencies of approximately 1 X 10(-5) to 1 X 10(-6).
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003013:Clostridium / Q000187:drug effects / Q000235:genetics*; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D011815:R Factors
D000900:Anti-Bacterial Agents