Gastrin induces intracellular Ca2+ release and acid secretion regulation by the microtubular-microfilamentous system.
In the absence of extracellular Ca2+ gastrin induced accumulation of amino [14C]pyrine as an index of acid secretion and evoked a cytosolic Ca2+ rise (measured by quin2/AM), suggesting that an intracellular Ca2+ release after the activation of the gastrinergic pathway may trigger acid secretion. In isolated parietal cells pretreatment with colchicine or cytochalasin B abolished the amino [14C]pyrine accumulation and the rise in cytosolic Ca2+ concentration evoked by gastrin. The results suggest that the microtubular-microfilamentous system regulates gastrin-induced intracellular Ca2+ release and acid secretion.
D000632:Aminopyrine / Q000378:metabolism; D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D008870:Microtubules / Q000378:metabolism; D010295:Parietal Cells, Gastric / Q000166:cytology / Q000187:drug effects
D000634:Aminoquinolines; D005755:Gastrins; D000632:Aminopyrine; D003571:Cytochalasin B; C035738:Quin2-acetoxymethyl ester; D003078:Colchicine; D002118:Calcium