[Production of hybridomas synthesizing monoclonal antibodies to streptococcal group A polysaccharide].
Nine stable hybridomas synthetizing monoclonal antibodies to the antigenic determinants of polysaccharide A of group A streptococcus were obtained. Three monoclonal antibodies possessed precipitating properties. The formation of hybridomas was found to be influenced by the presence of immune splenocytes and the standard conditions of cell fusion. The highest yield of hybridomas was observed under the conditions ensuring the growth of cell in 80-100% of the wells. Rapid and specific screening was found to be an important stage in obtaining hybridomas.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D002459:Cell Fusion; D002460:Cell Line; D003584:Cytological Techniques; D006825:Hybridomas / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010434:Pepsin A / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011135:Polysaccharides, Bacterial; D010434:Pepsin A
Poluchenie gibridom, sinteziruiushchikh monoklonal'nye antitela k polisakharidu streptokokka gruppy A.