Polymorphisms of mitochondrially encoded proteins.
Polymorphisms of mitochondrially encoded proteins can be detected in human lymphocytes by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Using an SDS-polyacrylamide 8 M urea system, 17 mitochondrially encoded proteins are distinguishable. Three of these (ME-6, ME-8, and ME-17) were polymorphic among 92 individuals screened, and these polymorphisms are reported here for the first time. With SDS-polyacrylamide electrophoresis without urea, 18 mitochondrial proteins are detectable. One of these (MV-1) varied in two of 31 individuals tested. This polymorphism has been identified previously in HeLa cells. Maternal inheritance of the ME-8 polymorphism was demonstrated by three informative families.
D004272:DNA, Mitochondrial / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D008928:Mitochondria / Q000032:analysis*; D011110:Polymorphism, Genetic
D004272:DNA, Mitochondrial; D008565:Membrane Proteins