title: Conservation of DNA sequences for plasmid-mediated citrate utilization within the enterobacteria.
abstract: Southern blot DNA-DNA hybridization experiments with a cloned Cit+ DNA fragment as a probe showed that the plasmid-mediated Cit+ determinants from four Cit plasmids (R726, pOH3001, pOH3035, and pOH30221) were all homologous. Sequences homologous to the plasmid-borne Cit+ gene were also found in total bacterial DNA isolated from Salmonella paratyphi B, Salmonella enteritidis, Salmonella typhimurium LT-2, Citrobacter freundii, ATCC 8090, Citrobacter amalonaticus ATCC 25405, Klebsiella pneumoniae I and IID 977, and Enterobacter aerogenes ATCC 13048. The DNA digest from C. amalonaticus ATCC 25405 contained a 1.4-kilobase BamHI-HincII DNA fragment that was strongly homologous with and identical in size to the plasmid Cit+ probe.
mesh_terms: D001483:Base Sequence; D002951:Citrates / Q000378:metabolism*; D019343:Citric Acid; D004269:DNA, Bacterial / Q000032:analysis*; D004755:Enterobacteriaceae / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002951:Citrates; D004269:DNA, Bacterial; D019343:Citric Acid