[Determination of serum apolipoproteins A1 and B by immunoturbidimetry using the Cobas-Bio centrifugal analyzer. Influence of hyperlipemia].
The authors describe an immunoturbidimetric technique for the determination of the serum apolipoproteins A1 and B using a Cobas-Bio centrifugal analyser. This methods is sensitive and reproducible (CV less than 5%) and show a good correlation with electroimmunodiffusion which was taken as method of reference. It may be applied to serum stored at 4 degrees C or frozen at -20 degrees C. No light is transmitted in the case of either turbid hyperlipemic sera or sera extensively icteric or hemolyzed. The physiological values found in a large population of men and women of various ages are in agreement with those reported in the literature. The method described thus provides a suitable means of investigation adapted to large series for screening for coronary disease states.
D000328:Adult; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood*; D001055:Apolipoproteins B / Q000097:blood*; D003327:Coronary Disease / Q000517:prevention & control; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D006951:Hyperlipoproteinemias / Q000097:blood; D007118:Immunoassay / Q000379:methods; D007122:Immunoelectrophoresis; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods; D014461:Ultracentrifugation / Q000295:instrumentation
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008075:Lipoproteins, HDL
Dosage des apolipoprotéines A1 et B sériques par immunoturbidimétrie sur analyseur centrifuge Cobas-Bio. Influence de l'hyperlipémie.