Detection of antibodies to Yersinia enterocolitica by single radial diffusion-in-gel and peroxidase labelled antibodies.
Sixty-nine sera with agglutinating antibodies to Y.enterocolitica were tested with a DIG-ELISA method using ethanol inactivated preparations of Y.enterocolitica (0:3 and 0:9). All sera with agglutination titer greater than or equal to 1280 were positive of which 71% were positive for IgM and IgA. Sera having lower titers by the agglutination technique were usually positive only for IgG antibodies. By titer 160 70% were positive using a conjugate directed against IgG, IgA and IgM. Further investigations are necessary to determine the clinical implication of the antibody-response measured within these immunoglobulin classes.
D000907:Antibodies, Bacterial / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D015008:Yersinia enterocolitica / Q000276:immunology*
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M