Purification of cystozoites of Sarcocystis sp. by the chromatographic gel Spheron.
Cystozoites of Sarcocystis sp. isolated from beef muscles were purified by elution through Spheron chromatographic gel. Using the number of cystozoites recovered and the purity of suspension as criteria, the effect of length of gel column, rate of elution, and ionic strength of the eluent was evaluated. The optimum purity was achieved at the elution rate of 10 ml/min and column length of 100 mm. The recovery of cystozoites (38-56%) was in correlation to the ionic strength of the eluent. Of the current isotonic solutions, Tyrode's solution was found to be the best eluent. Preliminary assays with different coccidia indicate that the method could be used for the study of a variety of parasitological topics.
D000818:Animals; D002415:Cats; D002417:Cattle; D002850:Chromatography, Gel; D004285:Dogs; D004947:Esophagus / Q000469:parasitology; D009130:Muscle, Smooth / Q000469:parasitology*; D009994:Osmolar Concentration; D012522:Sarcocystis / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification*