Rapid isolation of the 7S-nerve growth factor complex and its subunits from murine submaxillary glands and saliva.
7S-Nerve growth factor (NGF) and its alpha, beta-NGF, and gamma subunits have been purified from murine submaxillary glands and saliva by a combination of gel filtration on rigid polyvinyl gels, reversed-phase liquid chromatography on short alkyl chain supports (C4 columns), and ion-exchange chromatography on silica-based carboxymethyl columns. This technique is superior to previously used methods in that it is much more rapid and allows the purification of larger quantities of polypeptide from the same amount of starting material. Beta-NGF prepared with this method elicits the outgrowth of fibers of cells of a pheochromocytoma cell line (PC 12) in vitro, indicating that the biological activity is not impaired by the organic solvents and strong acids utilized for its isolation.
D000310:Adrenal Gland Neoplasms / Q000473:pathology; D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D046911:Macromolecular Substances; D051379:Mice; D009414:Nerve Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D010673:Pheochromocytoma / Q000473:pathology; D012463:Saliva / Q000032:analysis*; D013363:Submandibular Gland / Q000032:analysis*
D046911:Macromolecular Substances; D009414:Nerve Growth Factors