Computer-aided construction of nucleic acid restriction maps using defined vectors.
A new algorithm is described that will rapidly produce restriction maps of cloned DNA fragments. Information concerning the vector is stored as a data file and used in constructing probable maps. As the program is based upon a permutation analysis it has two primary uses. First, preliminary restriction maps can be created from fragment length data as a starting point for further analysis. Second, existing maps can be confirmed as being highly probable, and other probable maps examined to ensure certain combinations have not been overlooked. Although primarily designed for linear vectors, the program can be used to calculate circular maps.
D000465:Algorithms; D000818:Animals; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics; D005822:Genetic Vectors; D008838:Microcomputers; D015183:Restriction Mapping / Q000379:methods*