title: Adrenergic stimulation of pepsinogen release from rabbit isolated gastric glands.
abstract: Isolated gastric glands from the rabbit were used for studying the effect of catecholamines on the release of pepsinogen. Isoprenaline, adrenaline and noradrenaline stimulated pepsinogen release in a dose-dependent manner with similar maximal effects, but isoprenaline was significantly more potent than the other two agonists. The effect was mediated through beta-adrenoceptors, since the response was inhibited by the beta-adrenoceptor antagonist, propranolol, and since the alpha-adrenoceptor agonist, phenylephrine, was without effect in the concentration range 0.01 - 10 microM. Concentration-response curves for isoprenaline were shifted to the right in parallel by increasing doses of propranolol, and maximal response was not influenced by propranolol per se, which indicates a competitive type of antagonism. A Schild plot showed a pA2-value for propranolol of 7.70 and the slope of the regression line was 1.02. Studies with the beta 1-selective antagonist pafenolol and the beta 2-selective antagonist ICI 118.551 demonstrated that isoprenaline acted through beta 1-adrenoceptors. The results suggest an adrenergic component in the control of the peptic cells in rabbit gastric mucosa.
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000494:pharmacology*; D004837:Epinephrine / Q000378:metabolism; D005088:Exocrine Glands / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D010435:Pepsinogens / Q000378:metabolism*; D010656:Phenylephrine / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D011817:Rabbits; D013564:Sympathetic Nervous System / Q000502:physiology*; D013997:Time Factors
chemical_list: D002395:Catecholamines; D010435:Pepsinogens; D010656:Phenylephrine; D011433:Propranolol; D010434:Pepsin A; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine