Immunoaffinity purification of tissue plasminogen activator from serum-supplemented conditioned media using monoclonal antibody.
Tissue plasminogen activator (tPA) was purified from serum-supplemented culture media in a single-step immunoaffinity chromatographic procedure. This was done by producing a monoclonal antibody to tissue plasminogen activator. The antibody is a murine IgG1, kappa light chain molecule. The antibody was coupled to CNBr-activated Sepharose 4B. Tissue plasminogen activator was isolated from serum supplemented tissue culture media by immunoaffinity chromatography using a relatively high ionic strength equilibration buffer and an acidic elution buffer.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002459:Cell Fusion; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008545:Melanoma; D008970:Molecular Weight; D010053:Ovary; D010959:Tissue Plasminogen Activator / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D010959:Tissue Plasminogen Activator