Interaction of [14C]dimethylnitrosamine with albumin produced by rat hepatocytes in culture.
Rat hepatocyte cultures were incubated with [14C]dimethylnitrosamine (14C-NDMA) and the utility of measuring covalent binding of the radiolabel to newly synthesized albumin as a dose monitor of in vivo alkylation was determined. Hydrolysis of albumin, followed by derivatization and high performance liquid chromatography (HPLC) analysis of its constituent amino acids, showed radioactivity to be associated with a number of peaks. As the albumin fraction isolated from rat hepatocytes cultured with [14C]NDMA or [14C]methanol (14C-CH3OH) had similar profiles of radiolabelled amino acids it is concluded that the presence of radiolabel in albumin isolated from 14C-NDMA-treated hepatocytes is due to incorporation of its metabolic products rather than to direct alkylation.
D000418:Albumins / Q000096:biosynthesis*; D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002250:Carbon Radioisotopes / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004128:Dimethylnitrosamine / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D051381:Rats; D014316:Tritium / Q000378:metabolism
D000418:Albumins; D000596:Amino Acids; D002250:Carbon Radioisotopes; D014316:Tritium; D004128:Dimethylnitrosamine