title: Partial purification and characterization of eosinophil chemotactic factors from soluble extract of Fasciola species.
abstract: Eosinophil chemotactic factor (ECF) was partially purified from common liver flukes (Japanese strain of Fasciola sp) by a combination of anion-exchange chromatography on DE52 and gel filtration on Sephacryl S-200. The molecular weight of ECF was estimated to be approximately 27,000 by high-pressure liquid chromatography. The ECF was heat labile (56 and 100 C for 30 minutes) and was not bound with lentil-lectin Sepharose. The results of isoelectric focusing showed that ECF comprises at least 2 components; one was a major ECF with an isoelectric point of 3.1, and the other, a minor ECF with an isoelectric point of 3.8 to 4.5. These results indicate that ECF of common liver flukes are acidic proteins.
mesh_terms: D000818:Animals; D002630:Chemotactic Factors / Q000302:isolation & purification*; D002631:Chemotactic Factors, Eosinophil / Q000032:analysis / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005209:Fasciola / Q000032:analysis*; D007525:Isoelectric Focusing; D008970:Molecular Weight
chemical_list: D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil