Antibody determination against Aspergillus fumigatus by means of the enzyme-linked immunosorbent assay. II. Physico- and immunochemical properties of the polystyrene-binding components.
Polystyrene-binding antigenic components (PBC) of Aspergillus fumigatus involved in ELISA IgG antibody determinations and the corresponding numbers of precipitates found by double immunodiffusion (DID) and/or crossed immunoelectrophoresis (CIE) were studied using culture filtrate antigenic preparations fractionated by Sephadex G-100 chromatography, concanavalin A (ConA) affinity chromatography, isoelectric focusing (IEF) and TCA precipitation. It is shown that PBC, immunologically active in ELISA, are characterized by high molecular weights and ConA-binding properties. The pI values of these components were 3.5-6 and above 8. The glycoprotein and/or mucopolysaccharide nature is suggested by the binding to ConA and their partial resistance to trichloroacetic acid treatment. By contrast, low-molecular weight components and antigenic components that are not absorbed by ConA being active in DID and/or CIE showed less or absence of binding to the polystyrene surface.
D000908:Antibodies, Fungal / Q000032:analysis*; D001232:Aspergillus fumigatus / Q000276:immunology*; D003208:Concanavalin A / Q000378:metabolism / Q000494:pharmacology; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D008970:Molecular Weight; D011137:Polystyrenes / Q000378:metabolism
D000908:Antibodies, Fungal; D007074:Immunoglobulin G; D011137:Polystyrenes; D003208:Concanavalin A