Cloning of a cDNA encoding the smallest neurofilament protein from the rat.
We have cloned a cDNA coding for the smallest rat neurofilament protein. The cDNA is 861 nucleotides long coding for 287 amino acids from the internal alpha-helical region and the carboxy-terminal tail domain of the neurofilament protein. Comparison of the porcine, mouse and rat neurofilament protein sequences shows that the protein is highly conserved (greater than 93% identity). Blot analysis indicates that the cDNA is derived from a single neurofilament gene that codes for two different poly(A)+ mRNA species.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016900:Neurofilament Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D004247:DNA