Mechanism of factor VIII inactivation by human antibodies. III. Proteolytic cleavage of factor VIII:C and C antigen by thrombin.
Thrombin activation of factor VIII results in a marked, but transient, increase in factor VIII procoagulant activity. This proteolytic process has been examined by immobilizing factor VIII in a solid phase system using mouse monoclonal antibody specific for the factor VIII related antigen. These studies demonstrate that thrombin activation is the result of proteolytic cleavage from the factor VIII:C protein of a peptide fragment which contains both the factor VIII:C procoagulant and VIII:C antigen sites recognized by human antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal; D005169:Factor VIII / Q000276:immunology / Q000378:metabolism*; D015944:Factor VIIIa; D006801:Humans; D051379:Mice; D013917:Thrombin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015944:Factor VIIIa; D005169:Factor VIII; D013917:Thrombin