A catabolin-like factor from porcine kidney.
Explants of pig kidney cortex and medulla release a catabolin-like factor (CLF). The CLF of both kidney cortex and medulla can be precipitated with ammonium sulphate, mainly in the 60-95 per cent fraction. By gel chromatography the kidney CLF showed a major active fraction at a molecular weight of around 22 500. Whole glomeruli and dissociated glomerular cells in culture also released into the medium a CLF that could be bioassayed in live cartilage but displayed no effect on dead cartilage. The possible role of such a local hormone is discussed.
D000818:Animals; D002356:Cartilage / Q000378:metabolism; D002417:Cattle; D005591:Chemical Fractionation; D002809:Chondroitin Sulfates / Q000378:metabolism; D002850:Chromatography, Gel; D046508:Culture Techniques; D006025:Glycosaminoglycans / Q000378:metabolism; D007375:Interleukin-1 / Q000096:biosynthesis*; D053583:Interleukin-1beta; D007668:Kidney / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism; D013552:Swine; D013583:Synovial Membrane / Q000378:metabolism
D006025:Glycosaminoglycans; D007375:Interleukin-1; D053583:Interleukin-1beta; D002809:Chondroitin Sulfates