Complexes of IgG and plasma proteins in factor VIII preparations--a possible cause of adverse reactions.
IgG in factor VIII preparations was studied as a possible cause of adverse reactions following infusion of hemophiliacs. Thin-layer immuno-gel filtration analysis of nine products from seven firms demonstrated aggregated and monomeric IgG at highly variable amounts and proportions. These factor VIII products contained from 20 to 700 mg IgG per 1000 IU of factor VIII; IgG-fibrinogen complexes were demonstrated by double-antibody testing. A relationship is suggested between aggregated IgG and/or IgG complexes in factor VIII concentrates and adverse clinical reactions. They may also partially explain abnormalities and aberrations of the immune system previously reported in hemophiliacs.
D001798:Blood Proteins / Q000032:analysis*; D002850:Chromatography, Gel; D005169:Factor VIII / Q000009:adverse effects / Q000302:isolation & purification*; D005340:Fibrinogen / Q000032:analysis; D006467:Hemophilia A / Q000628:therapy*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D046911:Macromolecular Substances
D001798:Blood Proteins; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D005169:Factor VIII; D005340:Fibrinogen