An imbalance in sex chromosomes alters cell survival of human skin fibroblasts exposed to ionizing radiation in vitro.
We report that a cell clone with a 45,X(-Y) karyotype, obtained from a male individual who is a gene carrier for hereditary adenomatosis of the colon and rectum (ACR), was considerably more resistent to gamma-ray irradiation (D0 range 184-240) than were diploid ACR skin fibroblasts (SF) obtained from sister biopsies of the same individual or of normal controls (D0 range 90-125). In addition, cell clones obtained from a Turner's syndrome female patient and from a female patient with focal endometrial hyperplasia, each with a 45,X(-X) karyotype, were abnormally resistent to gamma-ray irradiation (D0 range 168-195). These results may suggest an association between the status of the sex chromosomes and an altered response to gamma-ray-induced cytotoxicity of human SF.
D000236:Adenoma / Q000235:genetics; D002470:Cell Survival / Q000528:radiation effects; D002869:Chromosome Aberrations; D002999:Clone Cells; D003110:Colonic Neoplasms / Q000235:genetics; D004247:DNA / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D007621:Karyotyping; D008297:Male; D011836:Radiation Tolerance; D012004:Rectal Neoplasms / Q000235:genetics; D012730:Sex Chromosomes; D012867:Skin / Q000528:radiation effects*
D004247:DNA