[The structure of chromatin from the pigeon brain. II. Sedimentation analysis of oligonucleosome density].
Compaction of pigeon brain and rat thymus chromatin differing in the length of the linker DNA has been studied by the method of velocity sedimentation. The dependence of sedimentation coefficients of oligonucleosomes on the number of nucleosomes in the chain in solution of different ionic strength (0.005-0.085) has been analyzed. The analyses of these dependences showed that the structure of oligonucleosomes of both cell types at low ionic conditions may be described by the model of a zig-zag-shaped nucleosomal chain. The process of compaction of the oligonucleosomes at higher ionic strength (0.045-0.085) proceeds similarly for brain and thymus chromatin. The formation of a superhelical structure is determined by the interaction of no less than 6 nucleosomes; the compactness of the structure is significantly increased when the number of nucleosomes in the chain exceeds 10. The ability of the brain oligonucleosomes to form a compact structure despite the short linker allow the suggestion that in brain short chromatin the DNA chain does not form two complete turns in the nucleosome. This provides necessary flexibility of brain chromatin.
D000818:Animals; D001923:Brain Chemistry; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis*; D010856:Columbidae; D006657:Histones / Q000032:analysis; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000032:analysis*; D009994:Osmolar Concentration; D011487:Protein Conformation; D051381:Rats; D013950:Thymus Gland / Q000032:analysis
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes
Struktura khromatina mozga golubia. II. Sedimentatsionnyĭ analiz kompaktizatsii oligonukleosom.