Evidence for a family of four immunochemically related isozymes of cytochrome P-450 purified from untreated rats.
Hepatic microsomal cytochromes P-450f, P-450g, P-450h, and P-450i have recently been purified to electrophoretic homogeneity from untreated adult rats and identified as distinct isozymes [Ryan et al. (1984) J. Biol. Chem. 259, 1239-1250; Haniu et al. (1984) Arch. Biochem. Biophys. 235, 304-311]. In Ouchterlony double-diffusion plates, as well as enzyme-linked immunosorbent assays and Western blots, purified immunoglobulin G (IgG) raised in rabbits against cytochromes P-450f or P-450g show strong cross-reactions with the heterologous protein, indicating apparent partial identity. Anti-P-450f and anti-P-450g also show strong cross-reactivity with cytochromes P-450h and P-450i but not with five previously characterized rat hepatic cytochromes, P-450 (i.e., P-450a-P-450e), indicating a high degree of immunochemical and structural relatedness among cytochromes P-450f, P-450g, P-450h, and P-450i.
D000818:Animals; D000918:Antibody Specificity; D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000302:isolation & purification*; D007120:Immunochemistry; D005779:Immunodiffusion; D007527:Isoenzymes / Q000145:classification / Q000302:isolation & purification*; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System