Selective extraction of the DM-20 brain proteolipid.
Brain DM-20 proteolipid was previously shown to be structurally different from the myelin major proteolipid (MMPL). In an attempt to set up a large-scale purification of DM-20, we studied extraction of brain proteolipids with mixtures of methylene chloride containing up to 80% methanol. The CH2Cl2-CH3OH (3:7, vol/vol) mixture is highly selective for the extraction of bovine brain DM-20 compared to MMPL. Purified DM-20 was obtained after chromatography of the extract on methylated Sephadex.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D000432:Methanol; D008752:Methylene Chloride; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D011510:Proteolipids; D051381:Rats
D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C489071:Plp1 protein, rat; D011510:Proteolipids; D008752:Methylene Chloride; D000432:Methanol