Detection of mutant diphtheria toxin-resistant Chinese hamster lung cells in situ by autoradiography.
An autoradiographic method was developed to detect diphtheria toxin-resistant (DTr) Chinese hamster lung cells in situ. With this method, a dose-dependent increase in the number of DTr cells was observed after exposure of the cells to increasing concentrations of ethyl methanesulfonate (EMS). Segregation of EMS-induced DTr cells in a sector of single colonies was clearly demonstrated. It was also found by this method that the number of DTr cells induced by a fixed concentration of EMS was not determined by the number of cell divisions during the expression time but by the period after exposure of the cells to EMS.
D000818:Animals; D001345:Autoradiography; D002455:Cell Division; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004167:Diphtheria Toxin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D008168:Lung / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation
D004167:Diphtheria Toxin; D005020:Ethyl Methanesulfonate