title: An electrode arrangement for horizontal electroblotting where gas bubbles are removed by a flow of buffer.
abstract: A wire electrode arrangement for a horizontal electroblotting apparatus is described. The electrodes were mounted in electrode conduits built on perforated plexiglass plates. Electroblotting was carried out in a buffer tank. Human serum proteins were separated by sodium dodecyl sulfate--polyacrylamide gel electrophoresis and electrophoretically transferred onto nitrocellulose membranes. Gas bubbles developed by electrolysis at the lower electrode were trapped in electrode conduits and removed by circulating buffer. Accumulation of gas bubbles between the electrodes during operation was avoided.
mesh_terms: D002021:Buffers; D003101:Collodion; D004566:Electrodes; D004586:Electrophoresis / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D012212:Rheology
chemical_list: D002021:Buffers; D003101:Collodion