Purification of anti-lactose antibodies from antisera to Neisseria gonorrhoeae and Neisseria meningitidis.
Lactosyl-sepharose 4B columns were used for purification by affinity chromatography of anti-lactose antibodies from rabbit antisera to the gonococcal strains 8551 and VII, and the serogroup B meningococcal strain M982. Anti-lactose antibodies were obtained from all three antisera. SDS-PAGE of lipopolysaccharide and of bacterial cells and immunoblotting with the anti-lactose antibodies showed that the lipopolysaccharide was the only bacterial component with binding sites for the antibodies.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D007785:Lactose / Q000276:immunology*; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D009345:Neisseria meningitidis / Q000276:immunology*; D011817:Rabbits
D000907:Antibodies, Bacterial; D007785:Lactose