title: FSH regulates cultured Leydig cell function via Sertoli cell proteins: an in vitro study.
abstract: The effects of follicular stimulating hormone (FSH) on testicular steroidogenic activity has been studied by testing the capacity of conditioned medium (CM) by both unstimulated (control) Sertoli cells (C-CM) and FSH stimulated Sertoli cells (FSH-CM) to influence porcine cultured Leydig cell activity. Leydig cells cultured in FSH-CM for 48 hrs, as compared to C-CM, show a significant (P less than 0.05) increase in [125I]-hCG binding (150% +/- 4) and hCG-stimulated testosterone (T) secretion (266% +/- 42). In addition, the stimulating effect of FSH-CM on Leydig cell function as compared to C-CM, is trypsin sensitive, non dialyzable, heat stable, acid resistant and is chromatographed following gel filtration (Sephadex G 100) into two different peaks of activity. These data suggest that FSH regulates Leydig cell function via (at least two types of) Sertoli cell secreted proteins.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003470:Culture Media / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology / Q000502:physiology*; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008297:Male; D011506:Proteins / Q000502:physiology*; D012708:Sertoli Cells / Q000187:drug effects / Q000502:physiology*; D013256:Steroids / Q000096:biosynthesis; D013552:Swine
chemical_list: D003470:Culture Media; D011506:Proteins; D013256:Steroids; D005640:Follicle Stimulating Hormone