Cold sensitivity in the transfer of a plasmid with a deletion hot spot into recombination deficient B. subtilis cells.
Various recombination-deficient mutants of B. subtilis, which are readily transformable by plasmid DNA at 42 degrees C cannot be transformed at 30 degrees C with chimeric plasmid derivatives that contain the deletion hot spot defined previously (Alonso and Trautner 1985a, b). Such interference was also observed in protoplast transformation and SPP1 transduction.
D001412:Bacillus subtilis / Q000235:genetics* / Q000648:ultrastructure; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D003080:Cold Temperature; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011995:Recombination, Genetic; D012093:Replicon; D014169:Transformation, Bacterial