The demonstration of the influenza virus by the method of the immune electron microscopy.
The direct and indirect method of the Immune electron microscopy (IEM) was used for the identification of two influenza viruses of the type A--[strain A/Chabarovsk/77 (H1N1) and A/Texas/77 (H3N2)]--by means of reference specific rat- and human- convalescent sera. In the cross-testing different dilutions of the virus in the allantoic fluid and of the sera were examined for the determination of the appropriate rates for the formation of the immunocomplexes. In the demonstration of the immunocomplexes the higher sensitivity of the indirect method was proved, by using the pig-anti-human-IgG serum,--in comparing with the direct method.
D000818:Animals; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000276:immunology; D002642:Chick Embryo; D003429:Cross Reactions; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G; D009980:Influenza A virus / Q000276:immunology; D008854:Microscopy, Electron; D009302:Nasopharyngeal Diseases / Q000276:immunology; D051381:Rats
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D007074:Immunoglobulin G