[The use of unsaturated phosphatidylcholines for determining the cholesterol esterase activity of biological matter].
A relatively simple method for preparation of a new substrate used in highly effective estimation of cholesterol esterase activity is described. Emulsion containing cholesterol I-14C oleate and unsaturated phosphatidyl cholines (70% linolic and 30% linolenic acids) was stabilized within three months.
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D002787:Sterol Esterase / Q000032:analysis*; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000494:pharmacology
D010713:Phosphatidylcholines; D013501:Surface-Active Agents; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase
Ispol'zovanie nenasyshchennykh fosfatidilkholinov dlia opredeleniia aktivnosti kholesterolésteraz v biologicheskom materiale.