Nucleotide sequence of the beta-lactamase gene of alkalophilic Bacillus sp. strain 170.
A gene for the beta-lactamase from alkalophilic Bacillus sp. strain 170 was cloned in a functional state on a 1.0 kb DNA fragment and its nucleotide sequence was determined. The coding sequence showed an open reading frame of 257 amino acids, which represents the beta-lactamase precursor protein. It is considered that the signal peptide consisted of 30 amino acids including 12 hydrophobic amino acids.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001345:Autoradiography; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001409:Bacillus cereus / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D021382:Protein Sorting Signals / Q000032:analysis; D001618:beta-Lactamases / Q000235:genetics*
D000596:Amino Acids; D004269:DNA, Bacterial; D021382:Protein Sorting Signals; D001618:beta-Lactamases