Comparison of ribosomes from gram-positive and gram-negative bacteria with respect to the presence of protein S1.
Ribosomes from Gram-positive and Gram-negative bacteria have been analysed for the presence of ribosomal protein S1 by three methods, sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoreaction with E. coli anti-S1 and chromatography on poly (U)-Sepharose. We observed that protein S1 is predominantly present in Gram-negative bacteria in comparison with Gram-positive bacteria. Exceptions are noted in both species.
D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006090:Gram-Negative Bacteria / Q000032:analysis*; D006094:Gram-Positive Bacteria / Q000032:analysis*; D005779:Immunodiffusion; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*; D013045:Species Specificity
D012269:Ribosomal Proteins; C015494:ribosomal protein S1