Supported phospholipid bilayers for two-dimensional protein crystallization.
Phospholipid bilayers, supported on UV irradiated carbon shadowed nitrocellulose electron microscope grids, have been used to induce two-dimensional crystal growth of IgE and IgG anti-DNP monoclonal antibodies. The UV irradiation renders the grids hydrophilic in a very uniform fashion and allows for the transfer of phospholipid monolayers from an air/water interface in a sequential dipping procedure. The surface coverage achieved was nearly 100% as measured by antibody binding and by the formation of protein arrays on the bilayer covered grids. The supported bilayers appear to be stably held and are appropriate for slow binding conditions and long incubation times with low concentrations of binding protein.
D000818:Animals; D000911:Antibodies, Monoclonal; D003460:Crystallization; D004140:Dinitrophenols / Q000276:immunology; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D008051:Lipid Bilayers; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D010743:Phospholipids; D011506:Proteins
D000911:Antibodies, Monoclonal; D004140:Dinitrophenols; D007074:Immunoglobulin G; D008051:Lipid Bilayers; D010743:Phospholipids; D011506:Proteins; D007073:Immunoglobulin E