Structure of hyperacetylated chromatin: light scattering and flow linear dichroism study.
Cation-induced folding of 10 nm chromatin filament to 30 nm fiber was studied with hyperacetylated chromatin using light scattering at 90 degrees and flow linear dichroism. Acetylated chromatin folded in a way indistinguishable from that of the control chromatin: both the compactness of chromatin and the orientation of nucleosomes relative to the fiber axis were identical at a given salt concentration.
D000107:Acetylation; D002843:Chromatin / Q000648:ultrastructure*; D006367:HeLa Cells / Q000648:ultrastructure; D006657:Histones / Q000378:metabolism; D006801:Humans; D008027:Light; D012542:Scattering, Radiation; D013057:Spectrum Analysis
D002843:Chromatin; D006657:Histones