A "dipstick" enzyme immunoassay for detection of antibody to Brucella abortus in cattle sera.
An enzyme immunoassay that utilizes antigen bound to a matrix which can be removed from the substrate to stop development is described. The assay which is performed in glass or plastic disposable tubes uses Gel-Bond film strips for attachment of antigen. The only equipment requirements are a rotary shaker and a spectrophotometer (optional). The antigen coated strips are passed through a series of tubes containing test serum, wash solution, antibody-enzyme conjugate, wash solution and substrate-chromogen taking about 45 minutes to perform. In testing sera with or without antibody to Brucella abortus a very high correlation existed between same day tests and tests performed over several days as well as with data on the same sera obtained by an enzyme immunoassay in a microtiter format.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis*; D002417:Cattle; D002846:Chromatography, Affinity; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008070:Lipopolysaccharides / Q000276:immunology
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides