title: The appearance of surface H-2 antigens on fetal and postnatal murine hepatocytes in vivo and in vitro: a comparative study of MHC control.
abstract: We have collected data on the kinetics of in vivo development of H-2b antigens in fetal and postnatal hepatocytes from days 15-89 postcoitum (pc) in C57BL/10 mice using a sensitive immunoferritin labeling method combined with electron microscopy. We compared these data with data on the kinetic development of H-2b antigens on fetal hepatocytes cultured from days 15, 16, and 17 pc onwards. Using the same techniques, we also compared the surface concentrations of H-2 antigens on the hepatocytes of pregnant and age-matched male and virgin female mice at day 110 pc. We found that the surface concentration of H-2 antigens on fetal and postnatal hepatocytes increased with time but that the birth event was associated with an increase in H-2 antigen expression from 60-80 ferritin grains per meter (F/m) to 190 F/m followed by a decrease to 115 F/m within 72 h of birth. The concentration of antigens on postnatal hepatocytes increased gradually and reached a maximum of 640 F/m on day 41 pc. By day 89 pc, however, this level had decreased 433 F/m, which was not significantly different from that found in day 110 pc males and virgin females. In contrast to previous findings, we found that hepatocytes cultured from days 15, 16, and 17 pc exhibited a large increase in H-2 surface antigen concentration within 48 h from 60-80 F/m to 1500-2200 F/m. After day 2, the H-2 concentration decreased and by days 14-17 in culture it was not significantly different from day 110 pc male and virgin female levels (485 F/m). Lastly, we found that the H-2 concentration on hepatocytes from pregnant mice was increased significantly (725 F/m) compared with the concentration of hepatocytes from day 110 pc males or virgin females. We postulate that the control of cell-surface major histo-compatibility complex antigen concentration is achieved by a combination of intra and extracellular mechanisms and we discuss how this might be of benefit to the animal in vivo.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006183:H-2 Antigens / Q000235:genetics; D008099:Liver / Q000196:embryology / Q000254:growth & development / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice
chemical_list: D006183:H-2 Antigens