title: The solubilization of platelet membrane-bound acetylcholinesterase and aryl acylamidase by exogenous or endogenous phosphatidylinositol specific phospholipase C.
abstract: Phosphatidylinositol specific phospholipase C from Staphylococcus aureus could solubilize acetylcholinesterase up to 55% from sheep platelets in the presence of ethylenediaminetetra acetic acid (EDTA). The endogenous phosphatidylinositol specific phospholipase C of platelets activated by deoxycholate (at 3-5 mM) could also solubilize the enzyme to a similar extent. The solubilized enzyme could be further purified to apparent homogeneity by affinity chromatography without the use of any detergents. It is suggested that phosphatidylinositol specific phospholipase C will be a useful tool in the solubilization of acetylcholinesterase from mammalian sources and its purification free of detergents. The present study also demonstrates the parallel behaviour of acetylcholinesterase and aryl acylamidase in platelets confirming their identity.
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood* / Q000302:isolation & purification; D000581:Amidohydrolases / Q000097:blood* / Q000302:isolation & purification; D000818:Animals; D001792:Blood Platelets / Q000201:enzymology*; D002846:Chromatography, Affinity; D003840:Deoxycholic Acid / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D012756:Sheep; D012995:Solubility; D010738:Type C Phospholipases / Q000494:pharmacology*
chemical_list: D003840:Deoxycholic Acid; D004492:Edetic Acid; D000110:Acetylcholinesterase; D010738:Type C Phospholipases; D000581:Amidohydrolases; C019732:aryl acylamidase