Increased production of aminoglycosides associated with amplified antibiotic resistance genes.
The 6'-N-acetyltransferase derived from Streptomyces kanamyceticus strain M1164 was cloned on to the high copy plasmid vector pIJ702 and introduced into S. kanamyceticus (ATCC 12853, a kanamycin producer) and S. fradiae (ATCC 10745, a neomycin producer). In both cases transformants containing the recombinant plasmid showed increased resistance to a number of aminoglycoside antibiotics and substantially increased production of kanamycin and neomycin. This demonstrates that specific amplification of gene products associated with antibiotic biosynthesis provides a means for improving antibiotic production.
D000123:Acetyltransferases / Q000235:genetics; D000617:Aminoglycosides / Q000096:biosynthesis*; D004352:Drug Resistance, Microbial; D005285:Fermentation; D005784:Gene Amplification; D007612:Kanamycin / Q000096:biosynthesis; D009355:Neomycin / Q000096:biosynthesis; D010957:Plasmids; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics
D000617:Aminoglycosides; D007612:Kanamycin; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; D009355:Neomycin