Evaluation of a spectrophotometric method for canine serum lipase determination.
The British antilewisite butyrate-dithionitrobenzoate (BALB-DTNB) spectrophotometric serum lipase assay was evaluated for precision, accuracy, and diagnostic usefulness in analyzing canine sera. Sera samples from clinically healthy dogs, dogs with experimentally induced pancreatitis, and dogs with spontaneous pancreatitis were analyzed. A titrimetric method of serum lipase determination was used for comparison. Although the BALB-DTNB method was not found to be precise or accurate for determining the lipase activity of canine serum samples, it seemed to be at least as diagnostically useful as the titrimetric procedure. The small sample size requirement and the speed of analysis of the BALB-DTNB procedure are advantages of this method over the titrimetric method, and thus, its use in place of the titrimetric method is justified. A laboratory reference range of 3 to 37 IU/L was determined for canine serum.
D000208:Acute Disease; D000818:Animals; D004796:Clinical Enzyme Tests; D004228:Dithionitrobenzoic Acid; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005069:Evaluation Studies as Topic; D007202:Indicators and Reagents; D008049:Lipase / Q000097:blood*; D008466:Mechlorethamine; D010195:Pancreatitis / Q000175:diagnosis / Q000662:veterinary*; D012016:Reference Values; D013053:Spectrophotometry / Q000379:methods
D007202:Indicators and Reagents; D008466:Mechlorethamine; D004228:Dithionitrobenzoic Acid; D008049:Lipase