Purification and identification of Streptomyces aureofaciens lD13 antibiotic.
Complete extraction of S. aureofaciens lD13 antibiotic was achieved by adding n-butanol to the clarified culture filtrate (v/2v) at pH 8.0. Using the column chromatography technique, 85.7% of the initial amount of the antibiotic was obtained in a purified form. Data of the Rf values of the antibiotic in different organic solvents revealed that it belongs to the tetracycline group. The antibiotic was chromatographically analyzed, using the thin-layer technique. UV and IR spectra, optical rotation, melting point as well as 15 colour reactions were also determined.
D020001:1-Butanol; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification*; D000440:Butanols; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D012997:Solvents; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013304:Streptomyces aureofaciens / Q000032:analysis*; D013752:Tetracycline / Q000032:analysis
D000900:Anti-Bacterial Agents; D000440:Butanols; D007202:Indicators and Reagents; D012997:Solvents; D020001:1-Butanol; D013752:Tetracycline