Genetic dissection of Drosophila adenylate cyclase.
The Drosophila learning mutant rutabaga is missing calcium/calmodulin activation of adenylate cyclase (EC4.6.1.1). The mutation was mapped at a finer resolution to X chromosome bands 12F5-7. By comparing wild-type and mutant cyclase activities, the relative responsiveness of the calcium-sensitive and calcium-insensitive components to different ligands could be determined; the calcium-sensitive fraction of the total cyclase activity was significantly less responsive to guanyl nucleotides and monoamines. The results suggest that the component of cyclase activity that is stimulated by calcium/calmodulin, possibly a genetically distinct catalytic subunit, is not coupled to the G subunit or the G subunit/monoamine receptor complex.
D000262:Adenylyl Cyclases / Q000235:genetics*; D000818:Animals; D001245:Association Learning / Q000502:physiology*; D001679:Biogenic Amines / Q000502:physiology; D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology; D002746:Chlorpromazine / Q000494:pharmacology; D002874:Chromosome Mapping; D004303:Dosage Compensation, Genetic; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology; D005796:Genes; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007858:Learning / Q000502:physiology*; D009154:Mutation
D001679:Biogenic Amines; D002147:Calmodulin; D006165:Guanylyl Imidodiphosphate; D000262:Adenylyl Cyclases; D002118:Calcium; D002746:Chlorpromazine