Oxygenation of phosphatidylcholine by human polymorphonuclear leukocyte 15-lipoxygenase.
A cell-free human polymorphonuclear leukocyte preparation containing both 15- and 5-lipoxygenase activities was found to oxygenate phosphatidylcholine at carbon-15 of the arachidonic acid moiety. No oxygenation at carbon-5 was found. Under similar incubation conditions, soybean and rabbit reticulocyte 15-lipoxygenases also oxygenated phosphatidylcholine, whereas rat basophilic leukemia cell 5-lipoxygenase, rabbit platelet 12-lipoxygenase and rat liver cytochrome P-450 preparations did not. Our results suggest that the oxygenation of phospholipids may be a unique property of the 15-lipoxygenases.
D000818:Animals; D001091:Arachidonate Lipoxygenases; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001491:Basophils / Q000201:enzymology; D001792:Blood Platelets / Q000201:enzymology; D002474:Cell-Free System; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006801:Humans; D008084:Lipoxygenase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology*; D010100:Oxygen; D010713:Phosphatidylcholines / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013329:Structure-Activity Relationship
D001095:Arachidonic Acids; D010713:Phosphatidylcholines; D016718:Arachidonic Acid; D003577:Cytochrome P-450 Enzyme System; D001091:Arachidonate Lipoxygenases; D008084:Lipoxygenase; D010100:Oxygen