Presence of a tubulin pool in the resting cyst of the hypotrich ciliate, Histriculus muscorum.
Although microtubular organelles have not been observed in the resting cyst of the ciliate, Histriculus muscorum, the cyst was immunocytochemically stained with anti-tubulin antiserum, and one of its polypeptide bands reacted to the same antiserum after the immunoblotting test. Both these findings demonstrate the presence of tubulin in the resting cyst. The reacted band appeared in the 100,000 g supernatant of the cyst but not in the pellet, suggesting a depolymerized state of the tubulin in the resting cyst.
D000818:Animals; D016798:Ciliophora / Q000166:cytology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D008297:Male; D008870:Microtubules / Q000032:analysis* / Q000502:physiology; D011817:Rabbits; D013768:Tetrahymena / Q000166:cytology / Q000378:metabolism; D014404:Tubulin / Q000032:analysis*
D014404:Tubulin