Relationship between acrosome reaction and in vitro fertilization of zona-free hamster eggs by bonnet monkey (Macaca radiata) spermatozoa.
The relationship between acrosome reaction, as studied by FITC-RCA staining technique, and the penetration of bonnet monkey spermatozoa into zona-free hamster eggs was investigated. The acrosomes of unreacted spermatozoa fluoresced, whereas those of acrosome-reacted sperm did not fluoresce owing to decreased binding of the lectin. The percentage of acrosome-reacted sperm increased following 3 h of incubation in BWW medium. The assessment of acrosome reaction by the FITC-RCA staining technique correlates well with the in vitro fertilization of zona-free hamster eggs.
D000177:Acrosome / Q000502:physiology*; D000818:Animals; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D037102:Lectins; D008255:Macaca radiata; D008297:Male; D037121:Plant Lectins; D013077:Sperm Head / Q000648:ultrastructure; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D013861:Thiocyanates
D005452:Fluoresceins; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate