Immunodiagnosis of bovine fascioliasis by enzyme-linked immunosorbent assay and immunoprecipitation methods.
Calves were infected with 1,000 Fasciola hepatica metacercariae and the antibodies which developed were monitored by gel diffusion, counterelectrophoresis (CEP) and the enzyme-linked immunosorbent assay (ELISA) using crude or tegument antigen preparations of the adult fluke. Serologic diagnosis was positive as early as 2-4 wk of infection by gel diffusion, by 4 wk by CEP, and by 6-8 wk by ELISA, whereas parasitologic diagnosis via the detection of fluke eggs in the feces was possible only after 8-10 wk of infection. In general, both gel diffusion and ELISA were superior to CEP in detecting active infection.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica / Q000276:immunology; D005211:Fascioliasis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D005779:Immunodiffusion; D013997:Time Factors
D000906:Antibodies