Modification of serine residues during sequential degradation of proteins and peptides by the phenylisothiocyanate method.
A novel method of serine determination during Edman degradation of proteins and peptides which is based on the reaction of serine degradation products with alkyl thiols is described. The physicochemical characteristics and the structures of the reaction products, i.e., of 5-[alkyl-thio)methyl)-3-phenyl-2-thioxo-4-imidazolinones, were determined. The procedure described permits the products of serine degradation to be obtained in a 70-80% yield.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D017879:Isothiocyanates; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides; D011506:Proteins; D012694:Serine / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013861:Thiocyanates
D007202:Indicators and Reagents; D017879:Isothiocyanates; D010455:Peptides; D011506:Proteins; D013861:Thiocyanates; C005441:phenylisothiocyanate; D012694:Serine