Creatine kinase: reassessment of optimal concentrations for adenosine-5'-diphosphate and magnesium.
Whereas univariate studies led to an European agreement for the choice of optimal reagent concentrations of 2 mmol/L for ADP and 10 mmol/L for Mg2+ for determining creatine kinase (EC 2.7.3.2) activity in serum, whatever its isoenzyme pattern, the results of our bivariate study led us to recommend higher optimal concentrations: 4.1 to 4.7 mmol/L for ADP and 22 mmol/L for Mg2+. The zone of maximal activity was in fact a broad plateau such that more than 99% of maximal enzyme activity was attained at ADP concentrations between 3 and 5 mmol/L and Mg2+ concentrations between 17 and 26 mmol/L. Under these new conditions the maximum activity measured was modestly increased (about 10%) over the previously recommended method but the assay could be expected to be more resistant to the variations of ADP and Mg2+ concentrations. It may become necessary to modify the European recommended method.
D000244:Adenosine Diphosphate; D002413:Cations, Divalent; D003402:Creatine Kinase / Q000097:blood*; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007527:Isoenzymes; D008274:Magnesium; D008433:Mathematics; D008962:Models, Theoretical
D002413:Cations, Divalent; D007527:Isoenzymes; D000244:Adenosine Diphosphate; D003402:Creatine Kinase; D008274:Magnesium