title: Generation of deletions through a cis-acting mutation in plasmid pC194.
abstract: When plasmid pC194-1 is ligated to pBR322 to generate plasmid pHV15-1, deletions occur with high frequency within the joined pBR322 DNA. Generation of deletions is recE4 independent, and occurs in B. subtilis with a 1,000-fold higher frequency than in Escherichia coli. In the hybrid plasmid pVH15-1, deletion end-points are not at random, but at defined locations within pBR322. We propose that the base alteration, characterizing pC194-1, has stabilized within the plasmid a stem/loop structure, which acts as a deletion generator.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D010957:Plasmids; D013696:Temperature; D014169:Transformation, Bacterial