An enzyme-linked immunosorbent assay for autoantibodies against the nuclear protein Scl-70.
This paper describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of autoantibodies against the nuclear protein Scl-70. The isolation of Scl-70 from rat livers and the conditions for the ELISA are described. Compared with the already established double diffusion in gel for detection of anti-Scl-70 antibodies this ELISA has advantages.
D000042:Absorption; D000818:Animals; D000941:Antigens / Q000276:immunology*; D034961:Antigens, Nuclear; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology* / Q000302:isolation & purification; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009698:Nucleoproteins / Q000276:immunology* / Q000302:isolation & purification; D010209:Paper; D051381:Rats; D012595:Scleroderma, Systemic / Q000276:immunology*
D000941:Antigens; D034961:Antigens, Nuclear; D001323:Autoantibodies; D001324:Autoantigens; D009698:Nucleoproteins; D003101:Collodion