Sensitive high-performance liquid chromatography system with fluorometric detection of three urinary catecholamines in the same range.
A simple and highly sensitive HPLC method for the simultaneous determination of three catecholamines (norepinephrine, epinephrine, and dopamine) was developed. Catecholamines were separated on a reversed-phase column, reacted with ethylenediamine at 70 degrees C and pH 8.2 for 4 min, and fluorometrically determined. Those detection limits were in the same range of 20 to 40 pg. When the method was applied to human urine samples, 3,4-dihydroxyphenylalanine, their precursor, was also determined.
D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004295:Dihydroxyphenylalanine / Q000652:urine; D004298:Dopamine / Q000652:urine; D004837:Epinephrine / Q000652:urine; D005029:Ethylenediamines; D006801:Humans; D009638:Norepinephrine / Q000652:urine; D013050:Spectrometry, Fluorescence
D002395:Catecholamines; D005029:Ethylenediamines; C031234:ethylenediamine; D004295:Dihydroxyphenylalanine; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine