title: Extracellular matrix-coated culture dishes: an in vitro model for primary human gynecologic tumors.
abstract: Culture dishes coated with an extracellular matrix (ECM) produced by bovine corneal endothelial cells were utilized to investigate human gynecologic carcinomas of epithelial origin. A high culture success rate was achieved for both solid tumor (83%) and ascitic fluid (75%) derived from specimens from 59 patients. Because of the high percentage of tumor cells attaching to the ECM and actively proliferating, a variety of in vitro studies (karyotypic analysis, morphologic appearance on ECM, and ability to digest the ECM) could be done. A preliminary in vitro profile of the various tumor cell types could be made on the basis of these studies. In addition, five long-term cultures were established utilizing the ECM model system.
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000648:ultrastructure; D002277:Carcinoma / Q000235:genetics / Q000648:ultrastructure*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000648:ultrastructure*; D005260:Female; D005833:Genital Neoplasms, Female / Q000235:genetics / Q000648:ultrastructure*; D006801:Humans; D007621:Karyotyping; D008954:Models, Biological; D010051:Ovarian Neoplasms / Q000235:genetics / Q000648:ultrastructure; D014594:Uterine Neoplasms / Q000235:genetics / Q000648:ultrastructure