Monoclonal antibodies to human vitamin D-binding protein.
Monoclonal antibodies to vitamin D-binding protein isolated from human serum have been produced. The antibodies obtained have been shown to be specific for human vitamin D-binding protein by three independent assays. The antibodies recognize human vitamin D-binding protein specifically in an enzyme-linked immunosorbent assay. Human vitamin D-binding protein is detected specifically in both pure and crude samples by a radiometric immunosorbent assay (RISA) and by an immunoprecipitation assay. The anti-human vitamin D-binding protein antibodies cross-react with monkey and pig vitamin D-binding protein, but not with vitamin D-binding protein from rat, mouse, or chicken, as determined by the RISA and immunoprecipitation assays.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002645:Chickens; D003429:Cross Reactions; D000882:Haplorhini; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D051381:Rats; D013045:Species Specificity; D013552:Swine; D014809:Vitamin D-Binding Protein / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014809:Vitamin D-Binding Protein