title: Use of fluorescent standards in protein transfer and immunoblotting. Accurate estimation of the molecular weight of immunoreactive species.
abstract: Fluorescent protein molecular weight markers have been used to standardize the electrotransfer and subsequent immunostaining of proteins separated by sodium dodecyl sulfate-polyacrylamide electrophoresis. The method permits accurate estimation of the molecular weight of immunoreactive species despite shrinkage or swelling which may occur during transfer or staining.
mesh_terms: D003101:Collodion; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007120:Immunochemistry; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D012015:Reference Standards; D013861:Thiocyanates
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins; D013861:Thiocyanates; D003101:Collodion; D016650:Fluorescein-5-isothiocyanate