A longitudinal slicer for obtaining multiple uniform slices from cylindrical polyacrylamide gels.
Construction of a longitudinal gel slicer for polyacrylamide gels is described. The apparatus produces intact, undamaged, and identical slices with rectangular cross section from gels of different thicknesses and different concentrations of acrylamide. When an extract containing brain proteins was electrophoresed on a cylindrical gel and then sliced and colored by Coomassie blue, an identical band pattern was obtained in all slices, indicating the absence of any gel distortion during cutting.
D000818:Animals; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation*; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes
D012394:Rosaniline Dyes; C048139:Coomassie blue