Simultaneous determination of N-acetylglucosamine, N-acetylgalactosamine, N-acetylglucosaminitol and N-acetylgalactosaminitol by gas-liquid chromatography.
A gas-liquid chromatographic procedure is described which will concomitantly separate and quantitate N-acetylglucosamine, N-acetylgalactosamine, N-acetylglucosaminitol and N-acetylgalactosaminitol in a single analytical run. The hexosamines, as their O-methyloximes, and the hexosaminitols can be separated as either their per-O-acetylated or per-O-trimethylsilylated derivatives. This method is particularly useful with samples that possess both N-acetylhexosaminitols and N-acetylhexosamines as are seen with N-linked oligosaccharides that are cleaved from glycoproteins by alkaline borohydride treatment. This procedure demonstrates a range of acceptable linearity of 1-1000 nmoles for each type of amino sugar.
D000116:Acetylgalactosamine / Q000031:analogs & derivatives* / Q000032:analysis*; D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000032:analysis*; D000818:Animals; D002849:Chromatography, Gas; D005688:Galactosamine / Q000031:analogs & derivatives*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins / Q000302:isolation & purification; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis; D013552:Swine
D006023:Glycoproteins; D009844:Oligosaccharides; C011631:N-acetylgalactosaminitol; C033828:N-acetylglucosaminitol; D005688:Galactosamine; D000116:Acetylgalactosamine; D005944:Glucosamine; D000117:Acetylglucosamine