Measurement of noradrenaline synthesis in rat hippocampal synaptosomes using HPLC with ECD.
A procedure to measure noradrenaline synthesis in rat hippocampal synaptosomes using high-performance liquid chromatography with electrochemical detection is described. In order to characterize the technique, the linearity of synthesis as well as the effects of K+-depolarization and tyrosine omission were investigated. The technique was compared with the radiochemical tritium release method and the DOPA-accumulation method.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004563:Electrochemistry; D006624:Hippocampus / Q000032:analysis* / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000032:analysis* / Q000096:biosynthesis; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000032:analysis; D014443:Tyrosine / Q000494:pharmacology
D014443:Tyrosine; D004295:Dihydroxyphenylalanine; D011188:Potassium; D009638:Norepinephrine