title: Mutagenesis of Clostridium acetobutylicum.
abstract: Mutagenesis of the obligate anaerobe Clostridium acetobutylicum was best accomplished using agents (e.g. ethyl methane sulphonate or N-methyl-N'-nitro-N-nitrosoguanidine) which are believed to act by a direct mutagenic mechanism. Other agents (e.g. u.v. radiation) whose effectiveness relies on misrepair of damaged DNA via an error-prone pathway, were poor mutagens of this organism. Procedures are described which readily yielded a variety of auxotrophic and other useful mutant strains of Cl. acetobutylicum and related saccharolytic clostridia.
mesh_terms: D003013:Clostridium / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D004260:DNA Repair; D005020:Ethyl Methanesulfonate; D008769:Methylnitronitrosoguanidine; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation
chemical_list: D008937:Mitomycins; D008769:Methylnitronitrosoguanidine; D016685:Mitomycin; D005020:Ethyl Methanesulfonate