title: Centromere spreading in a case of megaloblastic anemia "cured" under TC 199 culture conditions.
abstract: The present study discusses the possible reasons of appearance and disappearance of centromere spreading in chromosomes obtained from bone marrow cultures in RPMI 1640 and TC 199 media, respectively, in a case of megaloblastic anemia. This prevention or curing effect of centromeric region is suggested to have occurred due to exogenous thymidine present in TC 199 medium, probably reflecting the failure of proper DNA synthesis.
mesh_terms: D000368:Aged; D000748:Anemia, Macrocytic / Q000235:genetics*; D000749:Anemia, Megaloblastic / Q000235:genetics* / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D002503:Centromere / Q000648:ultrastructure*; D002873:Chromosome Fragility; D002875:Chromosomes / Q000648:ultrastructure*; D003470:Culture Media; D004261:DNA Replication; D006801:Humans; D008297:Male; D013936:Thymidine / Q000378:metabolism
chemical_list: D003470:Culture Media; D013936:Thymidine