[Cultivation of exo-erythrocytic schizonts of rodent Plasmodium in hepatocytes: a new experimental model for chemotherapy of malaria].
An in vitro experimental model using primary cultures of laboratory bred Thamnomys gazellae's hepatocytes and Plasmodium yoelii yoelii' sporozoites was set up for chemotherapeutic studies. The surface of the culture was reduced (0.5 cm) and allowed the rapid performance and analysis of schizonticide activity tests, with a reduced biological material (Rodents, Anopheles, sporozoites). Fifteen compounds were tested. When activity of molecules is known in vivo, both results in vivo and in vitro are parallel.
D000818:Animals; D000962:Antimalarials / Q000627:therapeutic use*; D003470:Culture Media; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004912:Erythrocytes; D008099:Liver / Q000469:parasitology*; D008288:Malaria / Q000188:drug therapy*; D010961:Plasmodium / Q000187:drug effects / Q000254:growth & development*; D012377:Rodentia
D000962:Antimalarials; D003470:Culture Media
La culture des schizontes exo-érythocytaires des Plasmodium de rongeurs dans des hépatocytes: un nouveau modèle expérimental pour la chimiothérapie du paludisme.