Inhibition of the heat shock response and synthesis of glucose-regulated proteins in Ca2+-deprived rat hepatoma cells.
Rat hepatoma cells become refractory to the induction of heat shock proteins and highly resistant to severe hyperthermia when incubated in Ca2+-free medium. The Ca2+-depleted cells synthesize polypeptides identified as the glucose-regulated proteins, but these proteins do not appear to be directly involved in the inhibition of the heat shock response. The results suggest that a Ca2+-dependent metabolic process is involved in the generation of the heat shock signal and/or mediates a step in the subsequent cascade of events that leads to the induction of heat shock protein synthesis and cell death.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002460:Cell Line; D006224:Cricetinae; D004533:Egtazic Acid / Q000494:pharmacology; D005947:Glucose / Q000502:physiology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats
D006360:Heat-Shock Proteins; D004533:Egtazic Acid; D005947:Glucose; D002118:Calcium