Mechanism of Ca2+ and dipicolinic acid requirement for L-alanine induced germination of Bacillus cereus BIS-59 spores.
Spores prepared from different sporulating media containing varying amounts of Ca and dipicolinic acid (DPA), exhibited differential responses to germination in L-alanine (0.25 M). Ca-spores with moderately high Ca and DPA contents could be triggered to germination by L-alanine, whereas P-spores with low contents of Ca and DPA could not be germinated by L-alanine unless Ca2+ or DPA was exogenously added. The initiation of L-alanine induced germination by P-spores in the presence of 45CaCl2 was associated with a marked uptake of 45Ca2+. Experiments involving stepwise extraction of 45Ca from prelabelled spores indicated that a part of the spore calcium may be involved in L-alanine induced germination. Both Ca2+ and DPA seemed to have a stimulatory effect on the incorporation of 14C-L-alanine.
D000409:Alanine / Q000378:metabolism / Q000494:pharmacology*; D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000032:analysis; D001409:Bacillus cereus / Q000201:enzymology / Q000502:physiology*; D002118:Calcium / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D010848:Picolinic Acids / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D013171:Spores, Bacterial
D010848:Picolinic Acids; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D000409:Alanine; D002118:Calcium; C004860:dipicolinic acid