title: Structure of sialyloligosaccharides isolated from bonnet monkey (Macaca radiata) cervical mucus glycoproteins exhibiting multiple blood group activities.
abstract: Mucin glycoproteins purified from cervical epithelial secretion of the bonnet monkey (Macaca radiata) exhibit multiple blood group activities. Alkaline borohydride reductive cleavage resulted in a mixture of neutral and acidic oligosaccharide-alditols. By high-performance liquid chromatography, seven oligosaccharides (A-4-1 to A-4-7) have been purified from the monosialyloligosaccharide fraction (A-4). Based on the results of 500-MHz 1H NMR spectroscopy, in conjunction with sugar analysis and immunological assays, we propose the following structures for these oligosaccharides. (formula: see text) These structures imply that either the A, B, or H determinant may be found in combination with the Cad/Sda determinant; the oligosaccharides identified, together, account for the blood group activities exhibited by the cervical mucus.
mesh_terms: D000818:Animals; D001789:Blood Group Antigens / Q000032:analysis*; D002240:Carbohydrate Sequence; D002582:Cervix Mucus / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004971:Estrus; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D008255:Macaca radiata; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000302:isolation & purification*
chemical_list: D001789:Blood Group Antigens; D006023:Glycoproteins; D009844:Oligosaccharides; C016735:sialooligosaccharides