Affinity chromatography of phospholipase A2 from Naja naja naja (Indian cobra) venom.
A rapid and improved purification procedure is described for phospholipase A2 from the Indian cobra, Naja naja naja. The procedure is based on affinity chromatography of the venom through Affi-Gel Blue to obtain a 9-fold purification in one step. However, as there are multiple forms of the enzyme in the venom and other proteins do bind to Affi-Gel Blue, further purification is achieved by DE-11 cellulose. Finally, a minor contaminant is removed by Sulfopropyl Sephadex C-25 chromatography. The resulting product was found to be pure by analytical isoelectric focusing and double diffusion precipitin tests. An isoelectric focusing column run with venom collected from a single snake gave a similar profile to that obtained with venom pooled from several snakes.
D000818:Animals; D002846:Chromatography, Affinity; D004546:Elapid Venoms / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D010740:Phospholipases / Q000032:analysis*; D010741:Phospholipases A / Q000032:analysis*; D054467:Phospholipases A2; D011506:Proteins / Q000032:analysis
D004546:Elapid Venoms; D011506:Proteins; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2