Encapsulation potential of a trypanocidal drug homidium bromide in bovine carrier erythrocytes.
Bovine red blood cells (RBC) were made to entrap homidium bromide, a trypanocidal drug. Hypotonic dialysis resulted in 0.3 mg drug encapsulated per milliliter of RBC. Homidium bromide had no untoward effects on the encapsulation of a marker 14C-sucrose. However, RBC containing homidium bromide had a mean cell volume (MCV) larger than that of sham-loaded cells. EDTA did not reverse the effects of the drug on the MCV. The in vitro drug efflux rate was 0.097 mg/h. The effects of drug on the 24-h circulating survival was determined, and 24-h cell survival was reduced from 80% to 50%.
D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability; D003692:Delayed-Action Preparations; D004492:Edetic Acid / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004996:Ethidium / Q000097:blood*; D008051:Lipid Bilayers; D008855:Microscopy, Electron, Scanning; D012710:Serum Albumin, Bovine; D014344:Trypanocidal Agents / Q000097:blood*
D003692:Delayed-Action Preparations; D008051:Lipid Bilayers; D014344:Trypanocidal Agents; D012710:Serum Albumin, Bovine; D004492:Edetic Acid; D004996:Ethidium