Hepatotoxic action of a poisonous mushroom, Amanita abrupta in mice and its toxic component.
An aqueous extract of a poisonous mushroom, Amanita abrupta was injected intraperitoneally into male ICR mice and the acute effects on the liver were studied. Contents of serum glucose and liver glycogen decreased to 60% and 10% of the control levels, respectively, 6 h after injection. Activities of serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase increased to 3- and 8-fold, respectively, 12 h after injection, and the elevated activities were maintained up to 24 h. Activities of the hepatic drug metabolizing enzymes were also reduced 15 h after injection. Histological examination demonstrated massive liver cell necrosis and disappearance of glycogen granules in the liver of the treated animals. Two amino acids, L-2-amino-4-pentynoic acid and L-2-amino-4,5-hexadienoic acid were identified in the mushroom extract. The former caused similar biochemical effects to those of the mushroom extract.
D000624:5-Aminolevulinate Synthetase / Q000378:metabolism*; D000363:Agaricales / Q000032:analysis; D000410:Alanine Transaminase / Q000097:blood; D000480:Alkynes / Q000633:toxicity; D000545:Amanita / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives / Q000633:toxicity; D006003:Glycogen / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D051379:Mice; D009336:Necrosis; D010936:Plant Extracts / Q000633:toxicity*; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000633:toxicity
D000480:Alkynes; D001786:Blood Glucose; D010936:Plant Extracts; C047863:2-amino-4,5-hexadienoic acid; C009055:propargylglycine; D006003:Glycogen; D000624:5-Aminolevulinate Synthetase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005998:Glycine; D013011:Sorbic Acid