Nucleotide sequence of the cohesive single-stranded ends of Bacillus subtilis temperate bacteriophage phi 105.
The cohesive single-stranded ends of temperate Bacillus subtilis phage phi 105 were analyzed with the exonuclease activities of the Klenow fragment of DNA polymerase I and with exonuclease III and were found to be 3' extensions. Chemical sequencing of 3'-end-labeled fragments showed that the ends are 7-base extended 3' single strands and have the sequence: 5'-GCGCTCC-3'. 3'-CGCGAGG-5'
D001412:Bacillus subtilis; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004256:DNA Polymerase I; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D005090:Exodeoxyribonucleases
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004256:DNA Polymerase I; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III