Incorporation of radiolabelled amino acids into the sulphur-containing metabolites of paracetamol by the hamster.
Various radiolabelled forms of the amino acids cysteine, methionine and serine were co-administered with paracetamol to the hamster to investigate the biosynthesis of the thiomethyl metabolites of paracetamol. T.l.c. and h.p.l.c. were used to determine the presence of sulphur-containing metabolites in the urine. Paracetamol sulphate and the 3-cysteine, mercapturate and thiomethyl derivatives were all detected in urine, along with the 3-methylsulphoxide after hydrolysis. No paracetamol-3-methylsulphone was detected. Both cysteine and methionine acted as sulphur donors via glutathione. The methyl group of the thiomethyl derivatives originated from methionine in a reaction system believed to involve cysteine conjugate-beta-lyase and the thiomethyl shunt.
D000082:Acetaminophen / Q000378:metabolism* / Q000652:urine; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D003545:Cysteine / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D008715:Methionine / Q000378:metabolism; D012694:Serine / Q000378:metabolism
D000596:Amino Acids; D000082:Acetaminophen; D012694:Serine; D008715:Methionine; D003545:Cysteine