Immunization of marmosets with Trypanosoma cruzi cell surface glycoprotein (GP90).
Marmosets (Callithrix jacchus) have been immunized with a vaccine comprising a Trypanosoma cruzi 90K cell surface glycoprotein and the adjuvant saponin; a combination previously shown to be protective in mice. Immunization was by two s.c. injections one month apart and non-lethal challenge of homologous Y strain T. cruzi was given one month after the booster immunization. No anti-T. cruzi antibodies were detected after the first immunization but high levels developed after boosting. Immunization caused a significant decrease in the levels of acute phase blood parasitaemia, however, both immunized and control animals remained xenodiagnosis positive 60 weeks after infection. No ECG aberrations, histopathological lesions or anti-tissue antibodies were detected in infected marmosets.
D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000276:immunology*; D002144:Callithrix; D002143:Callitrichinae; D014355:Chagas Disease / Q000469:parasitology / Q000517:prevention & control*; D004562:Electrocardiography; D006023:Glycoproteins / Q000276:immunology*; D007117:Immunization, Secondary; D008297:Male; D012503:Saponins; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000276:immunology*; D014611:Vaccination
D000276:Adjuvants, Immunologic; D000953:Antigens, Protozoan; D006023:Glycoproteins; D012503:Saponins