Binding of an aldose reductase inhibitor to renal glomeruli.
The aldose reductase inhibitor Sorbinil affects several membrane-associated complexes including Na/K-ATPase activity, transport processes, and impulse propagation. These considerations, coupled with the drug's aromatic nature, suggested the possibility of direct interaction with cell membranes. In the present study, binding of [3H]-Sorbinil to isolated glomeruli was demonstrated. Binding is dose-dependent and saturable, and can be inhibited by increasing concentrations of unlabeled Sorbinil. These results may help explain the compound's diverse effects on membrane-associated processes.
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D007093:Imidazoles / Q000378:metabolism* / Q000494:pharmacology; D048289:Imidazolidines; D066298:In Vitro Techniques; D007678:Kidney Glomerulus / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D013401:Sugar Alcohol Dehydrogenases / Q000037:antagonists & inhibitors*; D013402:Sugar Alcohols / Q000378:metabolism
D007093:Imidazoles; D048289:Imidazolidines; D013402:Sugar Alcohols; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; C026411:sorbinil