Identification of a major N-glycosylated protein of rabbit mammary gland and its appearance during development in vivo.
A major N-glycosylated protein was purified from hormonally stimulated pregnant and lactating rabbit mammary tissue. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, immunodiffusion and amino acid analysis showed the protein to be transferrin. The rate of synthesis of the protein during tissue development was studied and found to parallel the whey proteins casein and alpha-lactalbumin. The function of the protein during lactation is discussed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis / Q000302:isolation & purification*; D005779:Immunodiffusion; D007774:Lactation; D007930:Leucine / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008892:Milk / Q000032:analysis; D008894:Milk Proteins / Q000302:isolation & purification; D009924:Organ Culture Techniques; D011247:Pregnancy; D011817:Rabbits; D014316:Tritium
D000596:Amino Acids; D006023:Glycoproteins; D008894:Milk Proteins; D014316:Tritium; D007930:Leucine; D008358:Mannose