Analysis of specific protein synthesis by cultures of motor neuron-enriched cells from embryonic chicken using dual-label two-dimensional gel electrophoresis.
Protein synthesis in motor neuron-enriched and motor neuron-depleted cell cultures from chick embryo spinal cord have been examined by two-dimensional gel electrophoresis in conjunction with dual-label autoradiography. The majority of the 800 or so more abundant cellular proteins examined were found to be commonly expressed by both culture systems. A number of minor protein species were, however, clearly identified as being exclusively synthesized by motor neuron-enriched cell cultures and may thus comprise a small set of motor neuron-specific gene products.
D000818:Animals; D001345:Autoradiography; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D009046:Motor Neurons / Q000032:analysis* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D012967:Sodium Dodecyl Sulfate; D013116:Spinal Cord
D009419:Nerve Tissue Proteins; D012967:Sodium Dodecyl Sulfate