Mr determination of histones from their electrophoretic mobility in acidic urea gels in the absence of detergents.
The Mr of histones can be determined from their electrophoretic mobility at pH 2.3, 8 M urea in a polyacrylamide gel by correcting for differences in their charge density and properties of the gel matrix. The applicability of this method to other proteins is considered.
D000818:Animals; D002417:Cattle; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011489:Protein Denaturation; D012617:Sea Urchins; D012967:Sodium Dodecyl Sulfate; D013950:Thymus Gland; D014508:Urea
D003902:Detergents; D006657:Histones; D012967:Sodium Dodecyl Sulfate; D014508:Urea