Action of compounds with effective in vivo mineralocorticoid activity on ion transport in leucocytes.
We have studied the in vitro short-term effects of aldosterone (1.0-1000 nmol l-1), cortisol (0.5-5.0 mumol l-1), fludrocortisone (1.0-10 nmol l-1) and carbenoxolone (0.5-3 mmol l-1) on 86rubidium influx (a model for potassium), 22sodium efflux, and [3H]-ouabain binding capacity in intact human leucocytes. No effect of aldosterone (at concentrations present in Conn's syndrome) or fludrocortisone could be demonstrated on cation fluxes or [3H]-ouabain binding compared to controls. No significant effect of cortisol, at concentrations either physiological or present in Cushing's syndrome, could be demonstrated on cation fluxes or [3H]-ouabain binding compared to controls. Carbenoxolone significantly increased 86Rb influx and 22Na efflux at concentrations known to cause hypokalaemia in man. The effect was not blocked by propranolol. No effect could be demonstrated for [3H]-ouabain binding.
D000450:Aldosterone / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002229:Carbenoxolone / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008901:Mineralocorticoids / Q000494:pharmacology*; D010042:Ouabain / Q000378:metabolism; D011868:Radioisotopes; D012413:Rubidium; D012979:Sodium Radioisotopes; D013963:Thyroid Hormones / Q000494:pharmacology
D008901:Mineralocorticoids; D011868:Radioisotopes; D012979:Sodium Radioisotopes; D013963:Thyroid Hormones; D000450:Aldosterone; D010042:Ouabain; D012413:Rubidium; D002229:Carbenoxolone; D006854:Hydrocortisone