High-performance liquid chromatographic assay of antiinflammatory drugs incorporated in gel ointments. Separation and stability testing.
High-performance liquid chromatographic determinations of gabexate mesilate (FOY), prostaglandin E1 (PGE1), PGE1-alpha-cyclodextrin (PGE1-CD), prednisolone (PD) and butyl flufenamate (BF) incorporated in gel ointment were investigated. The gel ointment is composed of a carboxy vinyl polymer (1.3%, w/w) and a large amount of an aqueous organic solvent. A methanol extraction system offered simultaneous advantages of the removal of the polymer and the recovery of active ingredients from the gel phase. The recoveries of the drugs were 100%. The stabilities of PGE1, PGE1-CD, FOY, PD and BF incorporated in gel ointment, stored at 5, 25 and 40 degrees C for up to 90 days, were investigated.
D000527:Alprostadil / Q000032:analysis; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000032:analysis; D004355:Drug Stability; D005439:Flufenamic Acid / Q000031:analogs & derivatives / Q000032:analysis; D016670:Gabexate; D005782:Gels; D006146:Guanidines / Q000032:analysis; D009824:Ointments; D011239:Prednisolone / Q000032:analysis; D047391:alpha-Cyclodextrins
D000893:Anti-Inflammatory Agents; D003505:Cyclodextrins; D005782:Gels; D006146:Guanidines; D009824:Ointments; D047391:alpha-Cyclodextrins; D016670:Gabexate; D005439:Flufenamic Acid; C047051:ufenamate; D011239:Prednisolone; D000527:Alprostadil; C032613:alpha-cyclodextrin