An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells.
An enzyme immunoassay was developed to identify and quantify infectious murine parvovirus. The assay was as sensitive as the fluorescent focus test for quantification of virus in clinical material and was less labor-intensive. Viral infectivity could be inactivated with formalin prior to performance of the enzyme immunoassay. Specificity of the reaction product was shown by neutralization of infectivity using a hyperimmune rabbit antiserum to murine parvovirus.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005976:Glutaral / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D008915:Minute Virus of Mice / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D010321:Parvoviridae / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats
D005976:Glutaral