Reversed-phase liquid-chromatographic determination of purine compounds in serum applied to studies of hypouricemia.
With this rapid (20 min), sensitive (0.1 mumol/L), and reproducible reversed-phase "high-performance" liquid-chromatographic (HPLC) method hypoxanthine, xanthine, uric acid, allopurinol (4-hydroxypyrazolo[3,4-d]pyrimidine), and oxypurinol(4,6-dihydroxypyrazolo[3,4-d]pyrimidine) concentrations can be determined simultaneously in de-proteinized serum. We use an Amicon MPS-1 centrifugal ultrafiltration device, then inject the de-proteinized ultrafiltrate into a Toyosoda ODS-120A HPLC column and elute with a mobile phase consisting of potassium phosphate buffer (40 mmol/L, pH 2.2) containing 20 mL of methanol per liter. We used this method to investigate purine metabolism in 10 cases of hypouricemia (uric acid concentration in serum less than 60 mumol/L).
D000328:Adult; D000368:Aged; D000493:Allopurinol / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry; D005260:Female; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000097:blood; D008297:Male; D008659:Metabolic Diseases / Q000097:blood*; D008875:Middle Aged; D010117:Oxypurinol / Q000097:blood; D011687:Purines / Q000097:blood*; D014527:Uric Acid / Q000097:blood*; D019820:Xanthine; D014970:Xanthines / Q000097:blood
D007042:Hypoxanthines; D011687:Purines; D014970:Xanthines; D019820:Xanthine; D014527:Uric Acid; D019271:Hypoxanthine; D000493:Allopurinol; D010117:Oxypurinol