title: Circular dichroism observations on the lipoxygenase from reticulocytes.
abstract: CD spectra of reticulocyte lipoxygenase were recorded at various times during aerobic incubation, which leads to loss of catalytic activity, and after inactivation by the reaction product, 13-LS-hydroperoxylinoleic acid. Neither process showed any correlation with a decrease in the alpha-helix content, which was only observed over much longer times. It is concluded that inactivation of reticulocyte lipoxygenase by the suicidal reaction with 13-LS-hydroperoxylinoleic acid is not accompanied by gross structural alterations.
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D008041:Linoleic Acids / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D008433:Mathematics; D000432:Methanol; D011487:Protein Conformation; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*
chemical_list: D008041:Linoleic Acids; D008084:Lipoxygenase; C032890:13-hydroperoxylinoleic acid; D000432:Methanol