Hyperpolarizing membrane potential changes in a cloned monkey kidney cell line.
Electrophysiological properties of a cloned monkey kidney cell line, JTC-12, were studied. The mean resting potential and input resistance were -15.3 mV and 78 M omega, respectively. Spontaneous hyperpolarizations with increased membrane conductance were observed. Similar hyperpolarization could be elicited by mechanical and electrical stimulations. The mean reversal potential of these hyperpolarizations was -72.7 mV. Hyperpolarization could be also elicited in a chloride-free solution. These data indicate that: JTC-12 cells exhibit spontaneous and induced hyperpolarizations, and occurrence of hyperpolarization is related to an increase in membrane permeability to potassium ions.
D000818:Animals; D002463:Cell Membrane Permeability; D002999:Clone Cells; D004553:Electric Conductivity; D000882:Haplorhini; D007668:Kidney / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials; D011188:Potassium / Q000378:metabolism
D011188:Potassium