A simple nephelometric method for measuring the progressive motility and collecting motile spermatozoa.
A simple method for collection and measurement of the progressive motility of spermatozoa was developed. The procedure consists of the spectrophotometric measurement of the increase in optical absorbance produced by the upward migration of spermatozoa in a high density medium of Ficoll 400. The suspension containing migrated spermatozoa may be used as a purified population of uniformly motile and undamaged spermatozoa.
D000818:Animals; D002417:Cattle; D005362:Ficoll; D006801:Humans; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D013081:Sperm Motility
D005362:Ficoll