title: 31P-nuclear magnetic resonance analysis of extracts of vascular smooth muscle.
abstract: 31P-nuclear magnetic resonance spectroscopy was used to assess phosphate metabolites in perchloric acid extracts of rabbit aorta. In addition to the high energy phosphates, several other phosphorus compounds were detected and quantified. Most notable was the presence of a prominent phosphomonoester compound appearing at a chemical shift of 3.86 delta. This compound constituted 26% of the total extractable tissue phosphorus and is tentatively identified as ribose-5-phosphate, a pentose phosphate pathway intermediate. While ATP and phosphocreatine did not change during glucose and oxygen deprivation or during prolonged muscle contraction, the 3.86 delta phosphate decreased significantly. Furthermore, theophylline, an agent that increases intracellular cAMP, also decreased the level of the 3.86 delta phosphate. These results are consistent with the concept that intermediate metabolism sustains high energy phosphate pools in vascular smooth muscle in the steady state under various conditions. The pentose phosphate pathway may play an important role in vascular smooth muscle metabolism.
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic; D005947:Glucose / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000033:anatomy & histology*; D010100:Oxygen / Q000494:pharmacology; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D010759:Phosphorus Isotopes; D011817:Rabbits; D012267:Ribosemonophosphates / Q000032:analysis; D013806:Theophylline / Q000494:pharmacology
chemical_list: D010759:Phosphorus Isotopes; D012267:Ribosemonophosphates; C031626:ribose-5-phosphate; D013806:Theophylline; D005947:Glucose; D010100:Oxygen