The stimulation of prostaglandin production by transforming growth factor-alpha and 12-O-tetradecanoyl-phorbol-13-acetate or 1-oleoyl-2-acetyl-glycerol is synergistic.
Stimulation of prostaglandin production in C-9 rat liver cells by transforming growth factor (TGF)-alpha was synergistic with that of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). TGF-alpha and TPA synergized the release of radiolabelled compounds from [3H]arachidonic acid prelabelled C-9 cells. 1-Oleoyl-2-acetyl-glycerol (OAG) and TGF-alpha also synergized prostaglandin production in the C-9 cells suggesting that the tumor promoter was mimicking the physiological activator of protein kinase C, 1,2-diacylglycerol. TGF-alpha and TPA also synergistically stimulated arachidonic acid metabolism by NRK-49F rat kidney cells. In A-549 human lung carcinoma cells, TGF-beta, but not TGF-alpha, stimulated arachidonic acid metabolism synergistically with TPA.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D004075:Diglycerides / Q000494:pharmacology*; D004357:Drug Synergism; D005989:Glycerides / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007668:Kidney; D008099:Liver; D008168:Lung; D010455:Peptides / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015291:Transforming Growth Factors
D001095:Arachidonic Acids; D004075:Diglycerides; D005989:Glycerides; D006133:Growth Substances; D010455:Peptides; D010704:Phorbols; D011453:Prostaglandins; D016718:Arachidonic Acid; D015291:Transforming Growth Factors; D013755:Tetradecanoylphorbol Acetate