title: Glycogen synthase (casein) kinase-1: tissue distribution and subcellular localization.
abstract: The distribution of glycogen synthase (casein) kinase-1 (CK-1) among different rat tissues and subcellular fractions was investigated. Using casein, glycogen synthase and phosphorylase kinase as substrates, CK-1 activity was detected in kidney, spleen, liver, testis, lung, brain, heart, skeletal muscle and adipose tissue. The distribution of CK-1 among different subcellular fractions of rat liver was; cytosol (72.1%), microsome (17.6%), mitochondria (9.6%) and nuclei (0.7%). CK-1 from rat tissues was shown to have a similarly wide substrate specificity as highly purified CK-1 from rabbit skeletal muscle. Such wide substrate specificity and distribution among different mammalian tissues and subcellular organelles indicate that CK-1 may be involved in the regulation of diverse cellular functions.
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D001921:Brain / Q000201:enzymology; D047388:Casein Kinases; D002845:Chromatography / Q000379:methods; D006006:Glycogen Synthase / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013154:Spleen / Q000201:enzymology; D013347:Subcellular Fractions / Q000201:enzymology*; D013737:Testis / Q000201:enzymology
chemical_list: D006006:Glycogen Synthase; D011494:Protein Kinases; D047388:Casein Kinases