title: Characterization of Brucella abortus strain 19 isolated from human and bovine tissues and fluids.
abstract: One hundred isolates of Brucella abortus, which were recovered from bovine and human tissues or fluids, were identified as strain 19 by conventional bacteriologic methods. Each isolate was examined using a Warburg respirometer to determine oxidative rates on substrates of D- and L-alanine, L-glutamic acid, d(+)-galactose, D-ribose, and i-erythritol. These results were compared with those of repository (seed) cultures of strain 19 used for making antigens and vaccines. Except on the substrate of i-erythritol, each of the 100 isolates oxidized these substrates with rates different from the repository cultures and indistinguishable from those of field strains of B abortus. Thus, oxidatively, i-erythritol was the only substrate useful to help distinguish between strain 19 and virulent strains of B abortus biotype 1.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001431:Bacteriological Techniques; D001826:Body Fluids / Q000382:microbiology; D002003:Brucella abortus / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D002006:Brucellosis / Q000382:microbiology*; D002007:Brucellosis, Bovine / Q000382:microbiology*; D050260:Carbohydrate Metabolism; D002417:Cattle; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology; D010084:Oxidation-Reduction; D014774:Virulence
chemical_list: D000596:Amino Acids