Repair of chemical carcinogen-induced damage in DNA of human lymphocytes and lymphoid cell lines--studies of the kinetics of removal of O6-methylguanine and 3-methyladenine.
The removal of O6-methylguanine by human lymphoid cells corresponded, with certain assumptions, to a second-order chemical reaction in any given cell. There was a spectrum of proficiency in this respect for a considerable number of cells originating from different individuals and it was found that patients with diseases associated with autoimmunity tended to fall into the less proficient groups. E-B virus-induced lymphoid cell lines, derived from proficient, but not relatively deficient, peripheral blood lymphocytes, always (in 9/9 cases) reflected the level of proficiency of the donor lymphocytes with respect to removal of O6-methylguanine. Thus while proficient lymphocytes always produced proficient cell lines, deficient lymphocytes, in 3/8 cases, gave rise to more proficient cell lines. No evidence was found that groups of individuals exist who lack ability to remove 3-methyladenine from DNA, either from their blood lymphocytes or derived lymphoid cell lines.
D000225:Adenine / Q000031:analogs & derivatives* / Q000097:blood; D001327:Autoimmune Diseases / Q000097:blood / Q000235:genetics; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D004260:DNA Repair; D006147:Guanine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008433:Mathematics; D008956:Models, Chemical
D002273:Carcinogens; C025946:3-methyladenine; D006147:Guanine; C008449:O-(6)-methylguanine; D000225:Adenine