Effect of intracellular free Ca2+ on leucine transport in Chang liver cells.
Addition of Ca2+ ionophore (A23187) to the medium stimulated the Na+-independent leucine transport in Chang liver cells, increasing the cytoplasmic free Ca2+ concentration, irrespective of the presence or absence of extracellular Ca2+. Anticalmodulin drugs, such as chlorpromazine, trifluoperazine, and W-7, significantly inhibited the leucine transport in the cells. The stimulatory effect of A23187 on leucine transport was completely blocked in the presence of the anticalmodulin drug. Two microtubule disrupting drugs, colchicine and colcemid, significantly stimulated leucine transport. On the other hand, taxol, a microtubule stabilizing agent, decreased the stimulatory effect of colchicine on the leucine transport. These results strongly suggest the involvement of Ca2+ and calmodulin in regulation of Na+-independent leucine transport, possibly through control of assembly and disassembly of the microtubule network.
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D001826:Body Fluids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000494:pharmacology; D003703:Demecolcine; D004347:Drug Interactions; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects; D013449:Sulfonamides / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D013449:Sulfonamides; D014268:Trifluoperazine; D000001:Calcimycin; C017967:W 7; C034700:N-(6-aminohexyl)-1-naphthalenesulfonamide; D007930:Leucine; D002118:Calcium; D002746:Chlorpromazine; D003703:Demecolcine