Induction of IL 2 receptor expression and cytotoxicity of thymocytes by stimulation with TCF1.
We investigated the role of T cell cytotoxicity inducing factor 1 (TCF1) in the induction of a cytotoxic T cell response. We found that help-deficient thymocyte cultures supplied with saturating amounts of purified IL 2 did not develop CTL in a 5-day culture. The expression of cytotoxicity was dependent on the addition of TCF1 derived from the T cell hybridoma K15. TCF1 also induced proliferation of thymocytes in the presence of IL 2. Only the PNA- thymocyte subpopulation responded to TCF1 with proliferation and cytotoxicity in the presence of IL 2. The monokine IL 1 also induced proliferation in this subpopulation but failed to induce cytotoxicity. IL 1 was further distinguished from TCF1 by inhibition of IL 1-induced but not TCF1-induced proliferation by anti-IL 1 antibodies. In addition, using anti-IL 2 receptor antibodies (AMT 13), we showed that TCF1 in the presence of IL 2 substantially increased IL 2 receptor expression in thymocytes. IL 1 had the same effect on induction of IL 2 receptor expression as TCF1. Because some effects of IL 1 and TCF1 are distinct and some overlap, we discuss whether IL 1 and TCF1 induce different subsets of PNA- thymocytes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D002454:Cell Differentiation; D003602:Cytotoxicity, Immunologic; D007375:Interleukin-1 / Q000502:physiology; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000276:immunology; D015375:Receptors, Interleukin-2; D013234:Stem Cells / Q000166:cytology / Q000276:immunology; D013491:Suppressor Factors, Immunologic; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D013491:Suppressor Factors, Immunologic