A modified method to induce immune polyclonal ascites fluid in BALB/c mice using Sp2/0-Ag14 cells.
Immunized BALB/c mice were injected with 10(6) viable Sp2/0-Ag14 cells to induce ascites fluid concurrently with maximum serum antibody activity. Ascites fluids developed in a systematic manner, yielded large amounts of specific polyclonal antibody even when the mice were injected with relatively small amounts of immunogen. A combination of an initial pristane injection prior to 3 intraperitoneal immunizations of immunogen emulsified in Freund's complete adjuvant (total volume of 0.1-0.2 ml per injection), followed 4 days later by injection of 10(6) viable Sp2/0-Ag14 cells, resulted in a consistent production of ascites fluid with substantial specific antibody activity.
D000818:Animals; D000917:Antibody Formation; D001201:Ascites / Q000209:etiology; D001202:Ascitic Fluid / Q000276:immunology*; D002460:Cell Line; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000276:immunology; D007114:Immunization; D007117:Immunization, Secondary; D008221:Lymphoid Tissue / Q000166:cytology / Q000637:transplantation*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010534:Peritoneal Neoplasms / Q000150:complications / Q000209:etiology; D013729:Terpenes / Q000008:administration & dosage / Q000494:pharmacology
D013729:Terpenes; C009042:pristane; D005620:Freund's Adjuvant