title: Immunoglobulin transcripts and molecular history of a hybridoma that produces antibody to carcinoembryonic antigen.
abstract: Hybridoma CEA 66-E3 secretes only an antibody directed against human carcinoembryonic antigen (CEA). This hybridoma has been used as a source of mRNA for molecular cloning studies, and for the bacterial expression of anti-CEA heavy and light chains [Cabilly, S. et al., Proc. Natl. Acad. Sci. USA 81 (1984) 3273-3277]. We report here nucleotide sequence data which indicate that hybridoma CEA 66-E3 produces significant levels of several immunoglobulin transcripts in addition to those of anti-CEA. One kappa-chain transcript can be traced back to MOPC21; other transcripts probably come from genes derived from the antigen-stimulated normal lymphocyte. This information should be especially relevant for many hybridomas in which the immortalizing fusion partner is a derivative of MOPC21, for example, X63-Ag8,SP/0-Ag14, or NS1n.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005796:Genes; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D002272:Carcinoembryonic Antigen; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007136:Immunoglobulins; D012333:RNA, Messenger; D004247:DNA