Purification of antibodies for the cytokinin N6-(delta 2-isopentenyl) adenosine by affinity chromatography.
Isopentenyl adenosine antibodies useful in the investigations of the "cytokinin" functions of isopentenyl adenosine were purified by affinity chromatography. Using different affinity columns, the antibodies were purified to near complete purity. Analyses of the purified proteins revealed the presence of isopentenyl adenosine binding proteins in normal rabbit serum, which presence supports a suggested role for isopentenyl adenosine and its related compounds in animal cell division in vivo.
D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D003583:Cytokinins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007541:Isopentenyladenosine; D010937:Plant Growth Regulators / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine
D000906:Antibodies; D003583:Cytokinins; D010937:Plant Growth Regulators; C001478:N(6)-(delta(2)-isopentenyl)adenine; D012710:Serum Albumin, Bovine; D007541:Isopentenyladenosine; D000225:Adenine