Effect of dithiothreitol treatment of plasma on automated anti-D assay.
A study of automated anti-D quantitation on untreated and dithiothreitol (DTT) pre-treated plasma has been undertaken. The results indicate that in most cases pre-treatment of plasma with DTT causes a reduction in the anti-D level detected. In one case such treatment caused the anti-D level to fall to less than 50% of its original value. DTT treatment prior to quantitation may give a more accurate assessment of the IgG anti-D level present in plasma and thus be of assistance in the selection of suitable plasma for anti-D immunoglobulin preparation. The technique may also have applications in the diagnosis and management of haemolytic disease of the newborn.
D004229:Dithiothreitol; D004899:Erythroblastosis, Fetal / Q000175:diagnosis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007231:Infant, Newborn; D007518:Isoantibodies / Q000032:analysis*; D010949:Plasma / Q000276:immunology*; D011247:Pregnancy; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies; C033242:RH-antibodies; D012204:Rh-Hr Blood-Group System; D004229:Dithiothreitol