Increased actin nucleating activity in tumorigenic cells.
The kinetics of actin polymerization has been used to quantitate the relative levels of actin nucleating activity in extracts from a number of related tumorigenic and non-tumorigenic cells. The level of nucleating activity was significantly elevated in the tumorigenic compared with the non-tumorigenic cell extracts whether the results were expressed on the basis of per protein (2-3 fold increase) or per total endogenous cellular actin (3-4 fold increase). It is concluded that this activity is probably due to an actin filament capping/severing regulatory protein(s) and that this protein(s) may be, at least partially, responsible for the microfilament disruption observed in transformed cells.
D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D001704:Biopolymers; D002460:Cell Line; D006801:Humans; D007700:Kinetics; D008168:Lung; D008274:Magnesium / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000378:metabolism*
D000199:Actins; D001704:Biopolymers; D008274:Magnesium