Availability of hyperacetylated H4 histone in intact nucleosomes to specific antibodies.
Specific antibodies against the tetra-acetylated form of H4 histone have been elicited in the rabbit. They do not cross-react with the non-, mono-, and di-acetylated forms of the histone molecule but a slight cross-reactivity with the tri-acetylated form of H4 histone is observed. Our studies also show that hyperacetylated H4 histones are recognized by the antibodies in intact nucleosomes.
D000107:Acetylation; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D005450:Fluorescamine / Q000378:metabolism; D006657:Histones / Q000276:immunology*; D009707:Nucleosomes / Q000032:analysis*; D011817:Rabbits
D000906:Antibodies; D006657:Histones; D009707:Nucleosomes; D005450:Fluorescamine