Phenotypic and functional heterogeneity of human T lymphocytes producing B-cell growth factor(s): a clonal analysis.
Human T cells active on B-cell proliferation are phenotypically and functionally heterogeneous. A series of 43 human T-cell clones, selected according to their ability to release factors active on B-cell proliferation, were analyzed. B-Cell proliferation was evaluated by two different assays, namely, a costimulation assay with anti-mu antibody or by an assay based on B-cell preactivation with Staphylococcus aureus. Eight of these clones expressed the T4-/T8+ phenotype while the remaining were T4+/T8-. The large majority had T-cell growth factor activity as well. However, some clones appeared to have B-cell growth factor activity only. Fourteen clones (eight T8+ and six T4+) also displayed cytolytic activity in a phytohemagglutinin-dependent cytolytic assay.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006133:Growth Substances / Q000096:biosynthesis*; D006801:Humans; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D010641:Phenotype; D013211:Staphylococcus aureus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D006133:Growth Substances; D007148:Immunoglobulin mu-Chains; D008222:Lymphokines; D015847:Interleukin-4