Rabbit anti-human beta 2 microglobulin antibodies: a T-cell mitogen.
Rabbit anti-beta 2 microglobulin antibodies have been described as a potent mitogen for human B lymphocytes. However, when fractionated after activation, only the T-cell enriched population is actively dividing. The induction of proliferation in purified T cells requires the presence of non-T cells. Daudi cells (which do not express beta 2 microglobulin on their cell surface) were shown to produce 'macrophage replacing factors' which supported anti-beta 2-induced T cell division. Non-T cells could be effectively replaced by addition of interleukin 2 containing cell supernatants, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), or teleocidin B. Thus, anti-beta 2 appears to selectively activate T cells. In contrast to other T cell mitogens, anti-beta 2 microglobulin antibodies did not induce secondary immunoglobulin production in B cells from peripheral blood or spleen.
D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D000921:Antibody-Producing Cells / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology; D008235:Lyngbya Toxins / Q000494:pharmacology; D008934:Mitogens; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology*
D000906:Antibodies; D008222:Lymphokines; D008235:Lyngbya Toxins; D008934:Mitogens; D001613:beta 2-Microglobulin; C022176:teleocidins; D004247:DNA