title: [Neutralizing antibodies to human gamma-interferon].
abstract: Spleen lymphocytes from normal subjects who suddenly died were used as cells producing gamma-interferon. One spleen yielded (3-8) X 10(8) viable cells which made it possible to prepare from 3 to 81 of splenocyte suspension culture. Staphylococcal enterotoxin A (SEA) and B (SEB) were used as gamma-interferon inducers. Stimulation of splenocyte suspension culture with SEA and SEB resulted in production of gamma-interferon with an average activity of 640-2560 units/ml. A partially purified interferon preparation with an activity of 2 X 10(4) units/ml was obtained by sorption of gamma-interferon on porous glass CPG-200-240 followed by elution with a buffer containing 50% ethylene glycol and Sephadex G-25 gel chromatography. As a result of 11 successive intramuscular immunizations of rabbits at 2-week intervals with a partially purified and concentrated preparation of gamma-interferon with Freund's complete adjuvant, blood serum was obtained which was capable of neutralizing 32 units of gamma-interferon up to a dilution of 1:128. The serum was highly specific: it showed no specific interaction with antigenic determinants of either natural alpha- and beta- or plasmid alpha-F and alpha-F/D human interferons.
mesh_terms: D000328:Adult; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D002478:Cells, Cultured; D004768:Enterotoxins / Q000494:pharmacology; D006801:Humans; D007114:Immunization; D007369:Interferon Inducers; D007371:Interferon-gamma / Q000276:immunology*; D008875:Middle Aged; D009500:Neutralization Tests; D011817:Rabbits; D013154:Spleen / Q000187:drug effects / Q000276:immunology
chemical_list: D000906:Antibodies; D004768:Enterotoxins; D007369:Interferon Inducers; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma
vernacular_title: Neĭtralizuiushchie antitela k chelovecheskomu gamma-interferonu.