[Comparative determination of progesterone in the blood plasma by radioimmunological and chromato-mass-fragmentographic methods].
A comparative determination of progesterone in the blood plasma was performed using a radioimmunoassay (RIA) and 2 variants of a chromato-mass-fragmentographic (CMF) method. A deuterated internal standard, capillary gas-liquid chromatography and 3-enol-3-tret-BDMS and 3.20-bis-enol-bis-BDMS progesterone ethers as chromatographied derivatives were employed. Both derivatives made it possible to perform CMF determination using simplified variants of the method. The variants of the CMF method were employed as references for RIA of progesterone. Comparison of the results of the CMF and RIA methods indicated RIA significance (r = +0.99) in using antiserum to the conjugate 3-(O-carboxy methyl)-oxime of progesterone with bovine serum albumin injected to rabbits.
D005069:Evaluation Studies as Topic; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards; D006801:Humans; D011374:Progesterone / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards
D011374:Progesterone
Sravnitel'noe opredelenie progesterona v plazme krovi radioimmunologicheskim i khromato-mass-fragmentograficheskim metodami.