Application of pRPEL2 plasmid to detect African swine fever virus by DNA-DNA hybridization. Brief report.
A diagnostic assay has been developed to detect African Swine Fever virus in field samples. It is based on the use of 32P-DNA from pRPEL2 plasmid as a hybridization probe. This plasmid contains essentially the H-ClaI DNA fragment from the Spain-70 strain of African Swine Fever virus.
D000357:African Swine Fever / Q000175:diagnosis*; D000358:African Swine Fever Virus / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D007497:Iridoviridae / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013552:Swine
D004274:DNA, Recombinant; D004279:DNA, Viral