beta-Hydroxy acyl-CoA inhibition of mitochondrial ATP production.
beta-Hydroxypalmitoyl-CoA and beta-hydroxystearoyl-CoA were synthesized, purified and quantitated. beta-Hydroxypalmitoyl-CoA and beta-hydroxystearoyl-CoA instantly and reversibly inhibited oxidative phosphorylation by rabbit heart mitochondria oxidizing pyruvate. [8-14C]ADP uptake studies showed that the beta-hydroxy acyl-CoA species linearly inhibited the adenine nucleotide translocase system. Free beta-hydroxy fatty acids at comparable concentrations (0.005 mM) did not affect ADP uptake or state III respiration.
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D007700:Kinetics; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D011817:Rabbits; D013329:Structure-Activity Relationship
D000214:Acyl Coenzyme A; D010171:Palmitoyl Coenzyme A; C048143:3-hydroxyhexadecanoyl-coenzyme A; C033103:3-hydroxyoctadecanoyl-coenzyme A; D000255:Adenosine Triphosphate