Chlorantine fast green BLL as a stain for callose in oak phloem.
Sections of oak bark were stained with chlorantine fast green BLL, used as a 0.25% aqueous solution. All tissues were unstained, except for local deposits of material associated with phloem cell walls, which stained deep green. This green-staining material also stained specifically with resorcinol blue and with the aniline blue fluorescence technique, the usual histochemical tests for callose. The chlorantine fast green-staining material was removed from sections by treatment with a beta-1,3-glucan hydrolase. It is concluded that chlorantine fast green BLL stains callose in plant sections and is a useful additional stain for the histochemical detection of this polymer.
D001391:Azo Compounds; D002473:Cell Wall / Q000648:ultrastructure; D004396:Coloring Agents; D005936:Glucans / Q000032:analysis*; D059828:Plant Cells; D014197:Trees
D001391:Azo Compounds; D004396:Coloring Agents; D005936:Glucans; C048307:Chlorantine Fast Green BLL; C048306:callose