Effect of alkylation of tryptophan residues on the enzymatic and pharmacological properties of snake venom phospholipase A2.
Snake venom phospholipases A2 show a remarkable degree of amino acid sequence homology yet differ markedly in enzymatic and pharmacological activities. The basic phospholipase A2 from Naja nigricollis venom has much greater lethal potency, cardiotoxicity, hemolytic and anticoagulant activity than the acidic or neutral enzymes from Naja naja atra or Hemachatus haemachatus venoms, respectively, even though it has lower enzymatic activity than the latter two enzymes. Previous studies in which we selectively modified lysine and free carboxyl groups suggested that the pharmacological and enzymatic active sites are not identical. Tryptophan residues have been suggested as being involved in substrate binding although some phospholipases have no tryptophan. We investigated the effect of alkylating the tryptophans in N. nigricollis, N. n. atra, and H. haemachatus phospholipases A2 with 2-hydroxy-5-nitrobenzyl bromide. Chemical modification caused decreases in enzymatic activity, although the extent of inactivation varied with the enzyme and with the substrate (lecithin micelles, egg yolk, heart homogenates). The specificity of the enzymes for individual phospholipid substrates was not affected. Alkylation of the tryptophans also caused decreases in lethal, hemolytic, anticoagulant, and cardiotoxic potencies, which were similar to the extents of decrease in enzymatic activity. Our results suggest that tryptophans are not specifically associated with either the enzymatic or the pharmacological active site nor are essential for either activity.
D015077:2-Hydroxy-5-nitrobenzyl Bromide / Q000494:pharmacology*; D000478:Alkylation; D000818:Animals; D004546:Elapid Venoms; D004558:Electric Stimulation; D006352:Heart Ventricles / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D007700:Kinetics; D008297:Male; D009596:Nitrophenols / Q000494:pharmacology*; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism* / Q000633:toxicity; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains; D012911:Snakes; D013045:Species Specificity; D013379:Substrate Specificity; D014364:Tryptophan; D016276:Ventricular Function
D004546:Elapid Venoms; D009596:Nitrophenols; D015077:2-Hydroxy-5-nitrobenzyl Bromide; D014364:Tryptophan; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2