Variation in peripheral blood T cell subsets in serial assays.
No significant differences were found in the T cell subsets of fresh and frozen peripheral blood mononuclear cells (PBM) from six healthy donors analysed with the Ortho series of monoclonal antibodies and a fluorescence-activated cell sorter. Analysis of replicates of cryopreserved PBM showed that considerably higher variation in T cell subsets occurred when samples were assayed in serial assays than when the samples were analysed together under the same conditions. These results indicate that errors introduced into a longitudinal study by serial analysis of samples may be reduced if samples are cryopreserved and subsequently thawed and analysed together at the end of the study.
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D002469:Cell Separation; D005434:Flow Cytometry; D005615:Freezing; D006801:Humans; D011309:Preservation, Biological; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface