Immunological comparison of glyoxalase I from yeast and mammals and quantitative determination of the enzyme in human tissues by radioimmunoassay.
Antibodies to glyoxalase I from yeast, rat liver, porcine erythrocytes and human erythrocytes were raised in rabbits. Gel precipitation and immunotitration experiments demonstrated that the mammalian enzymes were immunologically related, but distinct from the yeast enzyme. Fab fragments of the antibodies to human glyoxalase I did not inhibit the catalytic activity, indicating that the antigen binding sites were not directed towards the active site of the enzyme. A radioimmunoassay for glyoxalase I was developed. Quantitative analysis of human adult as well as fetal organs demonstrated that glyoxalase I was present in a concentration of approximately 0.2 micrograms/mg protein in most human tissues.
D000328:Adult; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005333:Fetus; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007140:Immunoglobulin Fab Fragments; D007791:Lactoylglutathione Lyase / Q000032:analysis* / Q000097:blood / Q000276:immunology; D008099:Liver / Q000201:enzymology*; D008190:Lyases / Q000032:analysis*; D011247:Pregnancy; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013045:Species Specificity; D013347:Subcellular Fractions / Q000201:enzymology; D013552:Swine; D014018:Tissue Distribution
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D008190:Lyases; D007791:Lactoylglutathione Lyase