Inhibition of poly(ADP-ribose)polymerase causes increased DNA strand breaks without decreasing strand rejoining in alkylated HeLa cells.
Treatment of alkylated HeLa cells with 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase, increased the number of DNA strand breaks but did not affect the rate of strand rejoining. This suggests that an increase in DNA incision, not a decrease in ligation, results from the inhibition of poly(ADP-ribose) polymerase in cells recovering from DNA damaged by alkylating agents.
D000478:Alkylation; D001549:Benzamides / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000067856:Poly(ADP-ribose) Polymerase Inhibitors
D001549:Benzamides; D004273:DNA, Neoplasm; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D003561:Cytarabine; C025160:3-aminobenzamide; D004247:DNA