Hepatic clearance of tissue-type plasminogen activator in rats.
The clearance of tissue-type plasminogen activator (t-PA) was studied, in rats, by use of a functional assay for t-PA. Half-lives in the circulation were about one minute both for human (melanoma cell-derived) t-PA and for the rat's own t-PA. The clearance of t-PA required an intact liver blood flow. In isolated liver perfusion experiments the hepatic extraction of t-PA did not require any plasma proteins, including fast-acting t-PA inhibitor. Competition experiments, using monosaccharides, suggested that known hepatic glycoprotein receptors were not involved in hepatic t-PA extraction.
D000818:Animals; D008099:Liver / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate / Q000187:drug effects; D009005:Monosaccharides / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D013045:Species Specificity; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology
D009005:Monosaccharides; D014415:Tunicamycin; D010959:Tissue Plasminogen Activator