Effect of pyridoxalated stabilized stroma-free hemoglobin solution on the clearance of intravascular lipid by the reticuloendothelial system.
Although considerable work is being carried out with pyridoxalated glutaraldehyde-stabilized stroma-free hemoglobin solution (SFHS-pp) as a possible blood substitute, it is not known whether the SFHS-pp has any adverse effect on some aspects of reticuloendothelial (RE) function. To determine this, 131I-gelatinized trioleate (GT) was injected 30 min or 3 days after the injection of 45 or 100 mg/100 gm BW SFHS-pp (low and high dose, respectively). The results indicate that low as well as high dose SFHS-pp significantly decreased splenic uptake and transiently increased pulmonary uptake of GT. Hepatic retention of GT, however, increased 3 days following SFHS-pp administration, indicating that there was a stimulation of this aspect of liver RE function at that time. The intravascular clearance rates of GT at 3 days were also markedly faster, but no dose effect relationship was seen. This suggests that there was an overall stimulation of RE function 3 days following SFHS-pp administration. These results provide support for the safety of polymerized stroma-free hemoglobin solution for use as an oxygen-carrying volume support.
D000818:Animals; D005976:Glutaral; D006454:Hemoglobins; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008657:Metabolic Clearance Rate; D012157:Mononuclear Phagocyte System / Q000378:metabolism*; D011736:Pyridoxine; D051381:Rats; D012996:Solutions; D013154:Spleen / Q000378:metabolism; D013997:Time Factors
D006454:Hemoglobins; D012996:Solutions; D011736:Pyridoxine; D005976:Glutaral