Isolation of two trypsin inhibitors from resting seeds of the white bush (Cucurbita pepo var. patissonina) and their properties.
Two trypsin inhibitors, CPPTI-I and CPPTI-II of Mr 3 250 and 7 850, respectively, were isolated from resting white bush seeds. Both inhibitors are cysteine-rich proteins. In addition to trypsin, they inhibit a trypsin-like enzyme isolated from Streptomyces griseus proteinase but they do not act on chymotrypsin, kallikrein or subtilopeptidase A. The isolated inhibitors contain a lysine residue in position P1 of the reactive site.
D000596:Amino Acids / Q000032:analysis; D002845:Chromatography; D003545:Cysteine / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008239:Lysine / Q000032:analysis; D008970:Molecular Weight; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants / Q000032:analysis*; D011480:Protease Inhibitors / Q000494:pharmacology; D012639:Seeds; D013305:Streptomyces griseus / Q000201:enzymology; D014361:Trypsin Inhibitors / Q000302:isolation & purification*
D000596:Amino Acids; D010940:Plant Proteins; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; C031809:trypsin inhibitor, Cucurbita sp.; D008239:Lysine; D003545:Cysteine