[Isolation and characteristics of immobilized preparations of prostaglandin synthetase].
Enzymatic preparations of prostaglandin synthetase were immobilized by various methods, i.e., covalent binding to the carrier, incorporation into the matrix of polyacrylamide gel and adsorption on ion-exchangers. The most representative results were obtained with the use of the absorption technique. In this case, the enzyme activity either decreased very insignificantly or remained unaffected. The stability of immobilized enzyme preparations was studied. Some kinetic characteristics of immobilized prostaglandin synthetase were obtained.
D000327:Adsorption; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D015232:Dinoprostone; D004800:Enzymes, Immobilized / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000302:isolation & purification* / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis; D012669:Seminal Vesicles / Q000201:enzymology; D012756:Sheep
D001095:Arachidonic Acids; D004800:Enzymes, Immobilized; D011458:Prostaglandins E; D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone
Poluchenie i kharakteristika immobilizovannykh preparatov prostaglandinsintetazy.