Maintenance of the integrity of the blood-brain barrier in the rat during an in situ saline-based perfusion.
Integrity of the blood-brain barrier to the small polar tracer mannitol was maintained for up to 30 min during an in situ perfusion of the brain with a saline-based solution containing the metabolic inhibitor 2,4-dinitrophenol. The patency of the capillary bed after perfusion was demonstrated by injecting a solution of Indian ink and gelatin, and ultrastructural examination showed the microvasculature to be well preserved. These findings suggest that the blood-brain barrier can be studied under conditions that are independent of normal cerebral function and metabolism.
D019297:2,4-Dinitrophenol; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000098:blood supply; D002196:Capillaries / Q000187:drug effects / Q000648:ultrastructure; D004140:Dinitrophenols / Q000494:pharmacology; D008297:Male; D008353:Mannitol / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology
D004140:Dinitrophenols; D008353:Mannitol; D012965:Sodium Chloride; D019297:2,4-Dinitrophenol