A mutant from Escherichia coli which lacks ribosomal proteins S17 and L29 used to localize these two proteins on the ribosomal surface.
A mutant of Escherichia coli has been isolated which lacked ribosomal proteins S17 and L29, as judged by two-dimensional gel electrophoresis. A battery of immunological tests was used to confirm this result. Ribosomes of this mutant were used as a control for the localization of proteins S17 and L29 on the surface of the ribosomal subunits of E. coli. Protein S17 has been localized on the 30S subunit body, 3-5 nm away from the lower pole, while protein L29 is located at the back of the 50S particle on the opposite side to the interface.
D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D002499:Centrifugation, Density Gradient; D004588:Electrophoresis, Cellulose Acetate; D004926:Escherichia coli / Q000032:analysis / Q000235:genetics*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008854:Microscopy, Electron; D008958:Models, Molecular; D009154:Mutation; D012269:Ribosomal Proteins / Q000032:analysis* / Q000235:genetics; D013499:Surface Properties
D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C035078:ribosomal protein L29; C037084:ribosomal protein S17