Regulators of steroid secretion and inhibin activity in human ovarian follicular fluid.
Inhibin activity from human follicular fluid was purified by successive chromatographies on Sephadex G-50, DEAE-Biogel A and orange A dye matrix. Inhibin activity was associated only with the protein(s) that bound to orange A (OrA-2). Daily injection of OrA-2, 1 h prior to hMG into 10-day-old female rats for 4 days caused a significant inhibition of hMG-induced estradiol secretion. In vitro, OrA-2 dose-dependently inhibited the amounts of estradiol secreted by porcine granulosa cells during a 3-h incubation. Orange A-unbound proteins, on the other hand, induced a dose-dependent increase in estradiol as well as progesterone secretion by porcine granulosa cells in vitro. Separation of stimulator from the inhibitor by orange A chromatography led to an increase in the relative inhibin activity (25-50-fold) as well as aromatisation-suppressing activity (60-fold). The results indicate a possible local action of hFF inhibin to regulate aromatisation activity.
D000818:Animals; D001201:Ascites / Q000503:physiopathology; D001826:Body Fluids / Q000032:analysis / Q000502:physiology; D002369:Castration; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D007265:Inhibins / Q000302:isolation & purification* / Q000494:pharmacology; D010048:Ovarian Cysts / Q000503:physiopathology; D006080:Ovarian Follicle / Q000502:physiology*; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects
D006063:Chorionic Gonadotropin; D004958:Estradiol; D007265:Inhibins; D005640:Follicle Stimulating Hormone