Effects of strain and age on prophylaxis and co-carcinogenesis of urethan-induced mouse lung adenomas by butylated hydroxytoluene.
A single i.p. injection of butylated hydroxytoluene (BHT) 6 h before a single urethan injection had varying effects on lung tumorigenesis in mice of different strains and ages. Strains exhibiting both high (A/J, SWR/J) and low (BALB/cByJ, 129/J, C57BL/6J) susceptibility to urethan tumorigenesis were tested in this study. BHT treatment decreased tumor multiplicity by an average of 32% in adult A/J mice but acted as a cocarcinogen by increasing tumor number 48% in adult SWR/J mice, 240% in adult C57BL/6J mice, 655% in adult 129/J mice, and 38% in 14-day-old A/J mice. The numbers of both alveolar type 2 cell-derived and bronchiolar Clara cell-derived lung adenomas were similarly affected by these BHT treatments. Such BHT pre-treatment had no effect on adenoma multiplicity in either young or adult BALB/cByJ mice. Multiplicity in young BALB/cByJ mice was also unaffected by chronic BHT administration following urethan, even though multiplicities increased several-fold with such treatment in adult mice of this strain. Since the mice showing co-carcinogenesis by BHT include strains which are both highly susceptible and relatively resistant to urethan induction of lung tumors, our results support a distinction between genes regulating susceptibility to urethan carcinogenesis and to tumor modulation by BHT.
D000236:Adenoma / Q000139:chemically induced* / Q000517:prevention & control; D000367:Age Factors; D000818:Animals; D002084:Butylated Hydroxytoluene / Q000633:toxicity*; D003043:Cocarcinogenesis; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000517:prevention & control; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D014520:Urethane / Q000633:toxicity*
D002084:Butylated Hydroxytoluene; D014520:Urethane