Fluorometric determination of polystyrene latex: application to the measurement of phagosomes and phagocytosis.
Intrinsic fluorescence of polystyrene dissolved in organic solvents such as 1,2-dimethoxyethane was used to develop a sensitive method for the quantification of polystyrene latex beads. This method allows the assay of latex in the microgram range and is one order of magnitude more sensitive than the conventional spectrophotometric method. The fluorometric technique was used in the quantification of phagocytic latex particle uptake by macrophages and in the quantification of isolated phagosomal fractions.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism*; D011137:Polystyrenes / Q000032:analysis*; D012408:Rubber / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000378:metabolism
D011137:Polystyrenes; D012408:Rubber