v-src inhibits differentiation via an extracellular intermediate(s).
Murine 3T3L1 preadipocytes transformed by avian sarcoma virus were unable to differentiate in response to insulin or dexamethasone plus 1-methyl-3-isobutylxanthine, both potent inducers of differentiation of the nontransformed 3T3L1 parental line. Conditioned medium from transformed cells contained a relatively heat-stable factor(s) which inhibited the differentiation of untransformed parental 3T3L1 cells but did not induce any changes in their morphology. A protease-sensitive mitogen was also detected in the medium. The relationship between the two activities remains to be elucidated.
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D003470:Culture Media; D006133:Growth Substances / Q000502:physiology; D051379:Mice; D015688:Oncogene Protein pp60(v-src); D012191:Retroviridae Proteins / Q000502:physiology*
D003470:Culture Media; D006133:Growth Substances; D012191:Retroviridae Proteins; D015688:Oncogene Protein pp60(v-src)