title: Structural studies on the oligosaccharide moiety of the lipopeptidophosphoglycan from Trypanosoma cruzi.
abstract: The structure of the carbohydrate moiety of the lipopeptidophosphoglycan from Trypanosoma cruzi was studied by 13C NMR spectroscopy and by methylation analysis of the original and of an acid-degraded sample. An oligosaccharide, consisting of 2-O-substituted and 6-O-substituted mannoses, which is linked to the ceramide, was separated by partial acid hydrolysis from an external chain that contained 3-O-substituted mannopyranosyl residues. beta-D-Galactofuranosyl terminal units are attached to position 3 of (1----2)-linked mannopyranose. Besides the previously reported monosaccharide components (mannose, galactose, glucose and glucosamine), ribose was identified in a partial acid hydrolysate of the lipopeptidophosphoglycan. The last three sugars are minor components and their organization into the overall structure of the lipopeptidophosphoglycan has not been determined.
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D006027:Glycosides / Q000032:analysis; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis; D009904:Optical Rotation; D010457:Peptidoglycan / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D012266:Ribose / Q000032:analysis; D014349:Trypanosoma cruzi / Q000032:analysis*
chemical_list: D006027:Glycosides; D009844:Oligosaccharides; D010457:Peptidoglycan; D010743:Phospholipids; C014643:lipopeptidophosphoglycan; D012266:Ribose