Inhibitory effects of parathyroid hormone on growth of osteogenic sarcoma cells.
The effects of the bone resorbing hormone, parathyroid hormone (PTH), on the growth of malignant osteoblastic cells have been examined. The malignant osteoblastic cells were a clonal line (UMR 106) derived from a transplantable rat osteogenic sarcoma. The predominant effect of PTH at doses above 10(-10) M was an inhibition of replication and DNA synthesis. Replication was decreased by PTH in both the presence or absence of serum and at various cell seeding densities. Both bovine PTH (1-84) and the synthetic hormone, human PTH (1-34), inhibited replication, but with bovine hormone being an order of magnitude more potent. The effects could be observed in as short a time as 6 hours with DNA synthesis and 24 hours with replication.
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005312:Fetal Blood / Q000502:physiology; D006131:Growth Inhibitors / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects*; D012516:Osteosarcoma / Q000473:pathology*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors
D006131:Growth Inhibitors; D010281:Parathyroid Hormone