Platelet-activating factor mobilizes intracellular calcium in vascular smooth muscle cells.
The effect of platelet-activating factor (PAF) on polyphosphoinositide metabolism and 45Ca2+ efflux was examined in a vascular smooth muscle cell line (A7r5). PAF stimulated a rapid but transient production of inositol trisphosphate and inositol bisphosphate which, in the presence of lithium, resulted in an accumulation of inositol monophosphate. In addition, PAF induced a rapid efflux of 45Ca2+ from preloaded cells, an effect which was concentration-dependent. These data suggest that PAF mobilizes intracellular Ca2+ via the production of inositol trisphosphate.
D000818:Animals; D001011:Aorta; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000096:biosynthesis*; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000502:physiology*; D051381:Rats; D013403:Sugar Phosphates / Q000096:biosynthesis*
D007295:Inositol Phosphates; D010972:Platelet Activating Factor; D013403:Sugar Phosphates; D007294:Inositol; D008094:Lithium; D002118:Calcium