title: Separation of human plasma fibrin stabilizing factor (factor XIII) from fibrinogen by fibrinogen polymer formation.
abstract: Human plasma fibrin stabilizing factor (factor XIII) may be separated from fibrinogen through reversible fibrinogen polymer formation at pH 6.6, gamma/2 0.3, 0 degrees C, and subsequent Bio-Gel A 1.5m filtration. Factor XIII activity is eluted after the monomer fibrinogen peak. Polymer fractions from eight preparations, processed in duplicate, contain a mean 0.002 units factor XIII per mg fibrinogen, or about 0.7% the factor XIII content of standard plasma. Factor XIII-free fibrinogen polymers are easily dissociated (greater than 98%) to the monomer form by incubation at 37 degrees C, 18 hours. The fibrinogen preparations utilized were devoid of plasma fibronectin; thus these studies also show that reversible human fibrinogen polymer formation occurs in its absence.
mesh_terms: D002850:Chromatography, Gel; D005176:Factor XIII / Q000302:isolation & purification*; D005340:Fibrinogen / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D011108:Polymers; D011485:Protein Binding
chemical_list: D005353:Fibronectins; D011108:Polymers; D005340:Fibrinogen; D005176:Factor XIII