Identification and quantitation of O-phosphoserine in human plasma fibronectin.
O-Phosphoserine was positively identified as the phosphorylated moiety in human plasma fibronectin by 31P-NMR of intact peptides. These data correlated completely with chemical analyses which demonstrated the presence of O-phosphoserine at a concentration of 2 residues/molecule. Neither O-phosphothreonine nor O-phosphotyrosine was detected in partial acid and partial alkaline hydrolysates, respectively.
D005353:Fibronectins / Q000097:blood*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010446:Peptide Fragments / Q000032:analysis; D010768:Phosphoserine / Q000032:analysis*; D012694:Serine / Q000031:analogs & derivatives*; D013820:Thermolysin
D005353:Fibronectins; D010446:Peptide Fragments; D010768:Phosphoserine; D012694:Serine; D013820:Thermolysin