title: Human serotonectin: a blood glycoprotein that binds serotonin. Chemical and physiological characterization.
abstract: A glycoprotein that circulates in human blood, binds to the surface of platelets and white cells and also binds serotonin with high affinity and specificity has previously been purified and partially characterized. This glycoprotein has been called serotonectin. Antibodies raised against serotonectin inhibited the uptake of [3H]serotonin by platelets. We now report on the amino acid and carbohydrate composition of this protein as well as on some of the properties of the protein from which the carbohydrate moiety was removed. Serotonectin (apparent molecular weight 200 000; as judged by SDS-polyacrylamide gel electrophoresis) is an acidic protein that contains about 13% carbohydrate (w/w) consisting of mannose, galactose, glucosamine and sialic acid in a molar ratio of 2:1:4:0.8. Initial characterization suggests that serotonectin is a sialoglycoprotein of complex-type oligosaccharide N-linked to asparagine through N-acetylglucosamine. Treatment of serotonectin with neuraminidase resulted in a quantitative release of sialic acid without loss of antigenicity or binding capacity for [3H]serotonin. Treatment of desialylated serotonectin under non-denaturing conditions with almond glycopeptidase A resulted in 60-80% release of sugar. The protein moiety of the glycopeptidase-digested material showed no change in the capacity to bind [3H]serotonin and exhibited the same antigenic properties as untreated serotonectin. These data show the non-involvement of the carbohydrate moiety of human serotonectin in the mechanism of binding serotonin but the possible contribution of this moiety to a tighter interaction with the serotonectin receptor.
mesh_terms: D000581:Amidohydrolases; D000596:Amino Acids / Q000032:analysis; D001792:Blood Platelets / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D002849:Chromatography, Gas; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D019158:N-Acetylneuraminic Acid; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D011485:Protein Binding; D012701:Serotonin / Q000097:blood*; D050486:Serotonin Plasma Membrane Transport Proteins; D012794:Sialic Acids / Q000032:analysis
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D002352:Carrier Proteins; D006023:Glycoproteins; D050486:Serotonin Plasma Membrane Transport Proteins; D012794:Sialic Acids; D012701:Serotonin; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D019158:N-Acetylneuraminic Acid