Induction of IL 2 responsiveness in a murine IL 3-dependent cell line.
A mouse IL 3-dependent cell line, FD.C/1, can be induced to IL 2 growth responsiveness by culture in IL 2-conditioned culture medium. The IL 2-dependent cell lines derived by this procedure have been designated FD.C/2 cells. Once established, the FD.C/2 cells respond to human, rat, and mouse IL 2. When cultured with murine IL 3, FD.C/2 cells did not proliferate, appearing to accumulate in the G1 phase of the cell cycle. Other human and mouse lymphokines failed to stimulate FD.C/2 cell growth. The growth dependence of FD.C/2 cells on IL 2 could not be reversed to IL 3. Cell lines derived by these procedures could provide in vitro models for hemopoietic differentiative events.
D000042:Absorption; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D005434:Flow Cytometry; D006133:Growth Substances / Q000302:isolation & purification; D006168:Guinea Pigs; D006410:Hematopoiesis; D016298:Hematopoietic Cell Growth Factors; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism / Q000502:physiology*; D007377:Interleukin-3; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D014066:Palatine Tonsil / Q000166:cytology; D051381:Rats; D011994:Recombinant Proteins / Q000502:physiology; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism
D000954:Antigens, Surface; D003470:Culture Media; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007376:Interleukin-2; D007377:Interleukin-3; D008222:Lymphokines; D011994:Recombinant Proteins