[Preparation of smears of isolated hepatocytes and the glycogen preservation in them after cryopreservation of the liver tissue].
A method is proposed for preparation of smears of isolated hepatocytes from liver samples frozen in liquid nitrogen or dry ice. The thawing of liver samples and the preparation of smears of isolated hepatocytes were produced by a successive maintenance of the samples in a mixture of 0.067 M phosphate buffer and 5% sucrose (pH 8.0) at 20 degrees C, followed by placing in 0.067 M phosphate buffer (pH 7.4) at the same temperature. Then hepatocytes were fixed by methanol. The cytofluorometric analysis has shown that isolation of cells from the frozen and thawing liver tissue using the proposed method does not influence the level of glycogen preservation in the hepatocytes. The proposed method may be used in the clinical practice.
D000818:Animals; D002469:Cell Separation; D005434:Flow Cytometry; D005615:Freezing; D006801:Humans; D008099:Liver / Q000166:cytology*; D008112:Liver Glycogen / Q000032:analysis*; D008297:Male; D051381:Rats; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
D008112:Liver Glycogen
Poluchenie mazkov izolirovannykh gepatotsitov i sokhrannost' v nikh glikogena posle kriokonservatsii tkani pecheni.