Quantification of arabinogalactan-protein in plant extracts by single radial gel diffusion.
The amount of arabinogalactan-protein in whole plant extracts can be quantified by single radial diffusion in agarose gels containing a dye known as the beta-glucosyl-Yariv reagent which specifically interacts with and precipitates arabinogalactan-proteins. The lower limit of quantification is 0.04 microgram of arabinogalactan-protein; gum arabic is used as a standard reference arabinogalactan-protein. In principle, this method can be adapted to measure levels of any dye-precipitating macromolecule; for example, acidic polysaccharides can be estimated by their binding to the cationic dye Alcian blue.
D011232:Chemical Precipitation; D004396:Coloring Agents; D005779:Immunodiffusion; D007202:Indicators and Reagents; D008832:Microchemistry; D009088:Mucoproteins / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*
D004396:Coloring Agents; D007202:Indicators and Reagents; D009088:Mucoproteins; D010936:Plant Extracts; D010940:Plant Proteins; C006619:arabinogalactan proteins