An enzymatic method for calibration of serum lipase assays.
An enzymatic method for the calibration of a turbidimetric lipase assay is described, based on measurement of free fatty acids liberated by the action of lipase. The substrate of the turbidimetric assay is a colipase-containing triolein emulsion. For determination of the free fatty acids a commercial test kit including acyl-CoA synthetase, acyl-CoA oxidase, and peroxidase is used. Intra- and interassay imprecision (CV) is about 5% at above-normal lipase activities, about 10% at normal values. Temperature coefficients are 1.24 and 1.45, respectively, for measurements at 30 and 37 degrees C vs 25 degrees C.
D000818:Animals; D002138:Calibration; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D006801:Humans; D008049:Lipase / Q000097:blood*; D009391:Nephelometry and Turbidimetry; D013552:Swine; D013696:Temperature
D005230:Fatty Acids, Nonesterified; D008049:Lipase