The carbohydrates associated with the surfaces of hamster alveolar macrophages: a study using fluorescein-conjugated lectins.
Alveolar macrophages were isolated from the lungs of hamsters by lavage and centrifugation. The harvesting procedure yielded about 5.2 X 10(6) cells per animal and the majority of these cells were viable as assessed by a Trypan blue exclusion test. Monolayers of macrophages were labelled with a series of FITC-conjugated lectins. The results of this study suggest that the surface coating of the alveolar macrophages contains the following carbohydrate groups: N-acetylgalactosamine, N-acetylglucosamine, D-glucose, D-mannose, L-fucose and sialic acid.
D000116:Acetylgalactosamine / Q000378:metabolism; D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism; D006224:Cricetinae; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008647:Mesocricetus; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism; D013861:Thiocyanates / Q000378:metabolism
D005452:Fluoresceins; D037102:Lectins; D012794:Sialic Acids; D013861:Thiocyanates; D005643:Fucose; D016650:Fluorescein-5-isothiocyanate; D000116:Acetylgalactosamine; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose