Detection and separation of the submetacentric marker chromosome of the WALKER (W-256) carcinoma using flow cytometry and sorting.
The chromosomes of WALKER (W-256) carcinoma cells have been separated into different DNA subclasses using DAPI for quantitative DNA staining and laser flow cytometry. The submetacentric marker chromosome could be isolated and its DNA content was determined to be 1.3 pg. One microgram marker DNA was obtained after separation of about 750 000 marker chromosomes by means of electronic flow sorting. The chromosomal composition of sorted fractions was analyzed by microscopy following banding of sorted chromosomes. The average morphological purity obtained was about 83%.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics*; D002469:Cell Separation; D014128:Chromomycin A3; D002871:Chromosome Banding; D002875:Chromosomes / Q000032:analysis; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D005819:Genetic Markers; D007211:Indoles; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D005819:Genetic Markers; D007211:Indoles; C007293:DAPI; D004247:DNA; D014128:Chromomycin A3