Amino acid sequence of human plasma apolipoprotein C-III from normolipidemic subjects.
The complete amino acid sequence of human plasma apolipoprotein C-III (apoC-III) isolated from normal subjects is described. ApoC-III is a linear polypeptide chain of 79 amino acids. Tryptic digestion of intact apoC-III produced 5 major peptides, while tryptic digestion of the citraconylated protein yielded two peptides. The complete amino acid sequence of apoC-III was determined by the automated Edman degradation of the intact protein as well as the various tryptic peptides. Phenylthiohydantoin amino acids were identified by high-performance liquid chromatography and chemical ionization mass spectrometry. The amino acid sequence of apoC-III isolated from normolipidemic subjects is identical to the apoC-III sequence derived from the cDNA sequence and differs at 4 positions from the previously reported sequence of apoC-III derived from a patient with type V hyperlipoproteinemia.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006954:Hyperlipoproteinemia Type V / Q000097:blood; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D010446:Peptide Fragments; D014357:Trypsin