The relationship between the serogroup antigen and lipopolysaccharide of Legionella pneumophila.
Serogroup-specific antigen was extracted from a number of Legionella pneumophila strains and compared with phenol-water extracted lipopolysaccharide on the basis of gel filtration, chemical analysis, SDS-PAGE and reaction with serogroup-specific antibody in immunoblots. Serogroup specificity is apparently borne by the O side-chains of the lipopolysaccharide, which, although smooth in type, partitions in the phenol phase. For four serogroup 1 strains tested, there was no qualitative correlation between O side-chain length and pulmonary virulence for guinea-pigs.
D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007875:Legionella / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000032:analysis*; D012703:Serotyping; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides