Differentiation pathways of pluripotent hemopoietic stem cells in long-term mouse bone marrow culture.
Differentiation pathways of CFU-S were examined in mouse bone marrow long-term culture system. E/G ratios of non-adherent CFU-S in this system increased from 2 to 7-9 by 2-3 weeks of culture and then fluctuated during the culture period. On the other hand, E/G ratios of adherent CFU-S were lower than that of non-adherent CFU-S, and always remained under 2. Some of the supernatants collected from culture flasks at weekly intervals increased E/G ratios of normal bone marrow CFU-S after 18-20 h of incubation. These results suggest that the differentiation of CFU-S is controlled by humoral factors, secreted from yet unknown cells in bone marrow, in this system.
D000818:Animals; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D004912:Erythrocytes / Q000166:cytology; D004921:Erythropoietin / Q000032:analysis; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D008808:Mice, Inbred CBA
D003470:Culture Media; D004921:Erythropoietin