title: Intra-cellular and extra-cellular growth of L. monocytogenes in chick embryo fibroblast cell culture.
abstract: The growth of L. monocytogenes in isolated chick embryo fibroblast cell culture was studied. Hanks balanced salt solution and Eagles minimal essential medium were shown to support a limited growth of L. monocytogenes. Extra-cellular growth on the maintenance medium occurs for 48 h prior to the establishment of intra-cellular organisms. As the uptake of the parasite by the cell culture takes place, intra-cellular replication begins with subsequent release of the organisms into the surrounding medium. The organism continues to replicate intra-cellularly until all the cell culture is destroyed.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005347:Fibroblasts / Q000382:microbiology; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000378:metabolism; D013997:Time Factors