[Demonstration of succinate dehydrogenase activity in ascites tumor cells using fluorescent tetrazolium salts].
In Ascites tumor cells a fluorescent tetrazolium salt, new synthesized (Stellmach 1984) was tested on its suitability for the histochemical enzyme demonstration. Optimal incubation conditions for the demonstration of the succinate dehydrogenase were found out. The red coloured and red fluorescent formazane formed by enzymatic reduction can be localized as well under the light microscope as under the fluorescent microscope. A quantification of the formed formazane as a measure for the enzyme activity was possible by measuring the absorption and fluorescent intensity.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002460:Cell Line; D005456:Fluorescent Dyes; D005562:Formazans / Q000032:analysis; D006651:Histocytochemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D013385:Succinate Dehydrogenase / Q000032:analysis*; D013778:Tetrazolium Salts
D005456:Fluorescent Dyes; D005562:Formazans; D013778:Tetrazolium Salts; D013385:Succinate Dehydrogenase
Nachweis der Aktivität der Succinatdehydrogenase in Ascites-Tumor-zellen mit fluoreszierenden Tetrazoliumsalzen.