[Results of primary culture of malignant melanoma explants by the so-called compact covering lattices method].
Results from primary cultures of malignant melanomas explants can be amplified. The original method is based upon collagen lattices use. The collagen is retracted by human fibroblasts obtained from human angiomas. The cultured tumor fragments are covered by the lattices, which are now adhering to the flask. Six primitive malignant melanomas and three cutaneous metastases were cultured according to the classical method and the new method described here. Compact covering lattices allow a regular melanocytic redifferentiation either in primitive malignant melanomas or in cutaneous metastases. The results point out: the microenvironment's role in tumour growth; the different behaviour of primitive tumours and metastases.
D002478:Cells, Cultured; D008545:Melanoma / Q000473:pathology*; D008722:Methods
Résultats de la Primo-culture d'explants de mélanomes malins par la méthode dite des lattices compactes couvrantes.