Effect of the xid gene on graft-versus-host-induced autoantibody production in nonautoimmune mice.
Graft-versus-host disease (GVH) was used to induce an autoimmune state in F1 recipients using donor spleen cells, splenic T cells, or Lyt 1+2- splenic T cells from either normal DBA/2 mice or from DBA/2 mice carrying the X-linked immunodeficiency (xid) gene. Recipients were either nondefective (DBA/2 X CBA/N)F1 males or reciprocal cross (CBA/N X DBA/2)F1 male mice carrying the xid gene. GVH induced hypergammaglobulinemia and anti-ssDNA autoantibodies in F1 recipients. Immunodeficient (CBA/N X DBA/2)F1 recipients had less hypergammaglobulinemia and IgG anti-ssDNA than did normal (DBA/2 X CBA/N)F1 recipients. Spleen cells, splenic T cells, and Lyt 1+2- splenic T cells from immunodeficient DBA/2.xid donors were less able to induce GVH and autoimmunity than normal DBA/2 donors. These studies suggest that the xid gene may reduce the autoimmune hyperractive state, but may do so by acting on more than one cell population, including T cells.
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D001323:Autoantibodies / Q000096:biosynthesis*; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology*; D004247:DNA / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004247:DNA