The role of feeding rejection in Drosophila mutation assays.
The frequencies of sex-linked recessive lethal mutations recovered in the male post-meiotic germ cells of Drosophila after feeding on solutions containing a mutagen (either 1 mM methyl methanesulfonate, MMS or 2 mM ethyl methanesulfonate, EMS) and 5-bromo-2-deoxyuridine (BUdR) were significantly lower than the frequencies observed after treatment with the mutagen alone. In an attempt to explain the apparent 'protective' effect of BUdR, the feeding behavior of the flies was monitored for differences in the uptake of the mutagen-containing solution in the presence and absence of BUdR. This was accomplished by measuring the uptake of [14C]sucrose. The results indicated that the uptake of the feeding solution is inhibited by the presence of the selected concentration of BUdR (1.0 or 32.5 mM). Such a reduced uptake of the mutagen could alone account for the reduction in mutational yields noticed in treatments containing mutagen + BUdR compared to the ones with the mutagen alone. These results emphasize the need to monitor the feeding behavior of flies in experiments involving adult feeding.
D000284:Administration, Oral; D000818:Animals; D001973:Bromodeoxyuridine / Q000008:administration & dosage / Q000494:pharmacology; D003864:Depression, Chemical; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D004347:Drug Interactions; D005020:Ethyl Methanesulfonate / Q000008:administration & dosage / Q000494:pharmacology; D005247:Feeding Behavior / Q000187:drug effects*; D005804:Genes, Lethal; D008297:Male; D008741:Methyl Methanesulfonate / Q000008:administration & dosage / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000008:administration & dosage* / Q000494:pharmacology; D013649:Taste
D009153:Mutagens; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; D001973:Bromodeoxyuridine