The effect of calcium on cholesterol side-chain-cleavage enzyme in superovulated rat ovaries.
Cholesterol side-chain-cleavage enzyme activity in mitochondria isolated from heavily luteinized rat ovaries was determined using isocitrate as an electron donor and [4-14C]cholesterol to start the reaction. The activity of the enzyme was reduced when more than 10 microM calcium was added to the mitochondrial preparations. When 100 microM EGTA or 2 mM ATP was added to the reaction an increase in enzyme activity was observed and ATP was able to partially overcome the calcium-induced inhibition.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D007523:Isocitrates / Q000494:pharmacology; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D010053:Ovary / Q000201:enzymology* / Q000378:metabolism; D010060:Ovulation; D010088:Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D013480:Superovulation
D007523:Isocitrates; D013386:Succinates; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D003513:Cycloheximide; C034219:isocitric acid; D019802:Succinic Acid; D010088:Oxidoreductases; D002786:Cholesterol Side-Chain Cleavage Enzyme; D002118:Calcium