Electrophysiological correlates of peroxide damage in guinea pig hippocampus in vitro.
To study the effects of active oxygen on neuronal electrophysiology, hippocampal brain slices were exposed to hydrogen peroxide plus ferrous sulfate which react to produce hydroxyl free radicals. Analysis of extracellularly recorded somatic and dendritic responses to orthodromic stimulation indicated a decrease in both synaptic efficacy and impairment of action potential generation.
D000818:Animals; D001927:Brain Diseases / Q000139:chemically induced; D002545:Brain Ischemia / Q000503:physiopathology; D004195:Disease Models, Animal; D004347:Drug Interactions; D005071:Evoked Potentials / Q000187:drug effects; D005296:Ferrous Compounds / Q000633:toxicity*; D005609:Free Radicals; D006168:Guinea Pigs; D006624:Hippocampus; D006861:Hydrogen Peroxide / Q000633:toxicity*; D066298:In Vitro Techniques; D007501:Iron / Q000633:toxicity*; D008054:Lipid Peroxides / Q000096:biosynthesis; D008297:Male
D005296:Ferrous Compounds; D005609:Free Radicals; D008054:Lipid Peroxides; C020748:ferrous sulfate; D006861:Hydrogen Peroxide; D007501:Iron