An improved method for the analysis of sister-chromatid exchange in vivo.
A single injection method of halogenated nucleosides for analysis in vivo of SCE is reported. Halogenated nucleosides were suspended in plant oils, such as peanut oil, and injected into mice subcutaneously. When the dosage of halogenated nucleosides reached 500 mg/kg, satisfactory differential sister chromatid staining of bone marrow cells was obtained. This technique was simple, neither special equipment nor surgical procedure was needed, and the dosage of halogenated nucleosides was relatively low.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002453:Cell Cycle; D003520:Cyclophosphamide / Q000494:pharmacology; D007065:Idoxuridine / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods; D009821:Oils; D000074241:Peanut Oil; D010938:Plant Oils; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000348:Aflatoxins; D008937:Mitomycins; D009821:Oils; D000074241:Peanut Oil; D010938:Plant Oils; D016685:Mitomycin; D003520:Cyclophosphamide; D016604:Aflatoxin B1; D007065:Idoxuridine