Interactions of cytotoxic and other drugs: rapid cell culture assay.
In order to investigate the interactions of cytotoxic and other drugs, a cell culture assay was devised where changes in the ability of cytotoxic drugs to induce cytopathogenic effects can easily be visualized. A human mammary carcinoma cell line (BT 20) and a human hypernephroma cell line were used. Seventeen commonly used cytotoxic drugs were titrated in this assay. As an example of drug interactions the neutralization of nitrogen mustard, cis-dichlorodiammine-platinumII, and 4-hydroperoxycyclophosphamide by thiol-containing drugs shows that this assay is well suited to detect interactions of cytotoxic and other drugs.
D000970:Antineoplastic Agents / Q000037:antagonists & inhibitors / Q000627:therapeutic use*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D001943:Breast Neoplasms; D002292:Carcinoma, Renal Cell; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin; D003520:Cyclophosphamide; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004347:Drug Interactions; D006801:Humans; D008466:Mechlorethamine; D013438:Sulfhydryl Compounds
D000970:Antineoplastic Agents; D013438:Sulfhydryl Compounds; D008466:Mechlorethamine; D003520:Cyclophosphamide; D002945:Cisplatin