Interleukin-2 induces rapid phosphorylation of an 85 kilodalton protein in permeabilized lymphocytes.
Lymphocytes were induced to express receptors for interleukin-2 by stimulation for 5 days with phytohaemagglutinin and subsequently permeabilized by treatment with L-alpha-lysophosphatidylcholine. Phosphorylation of an 85 kDa protein was stimulated when these cells were treated with interleukin-2 or with an antibody directed against the interleukin-2 receptor.
D002463:Cell Membrane Permeability / Q000187:drug effects; D006801:Humans; D007158:Immunologic Techniques; D007163:Immunosorbent Techniques; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology*; D008214:Lymphocytes / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D010750:Phosphoproteins / Q000097:blood*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism; D015375:Receptors, Interleukin-2
D007376:Interleukin-2; D008244:Lysophosphatidylcholines; D010750:Phosphoproteins; D010835:Phytohemagglutinins; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D011493:Protein Kinase C