Automatic classification of chromosomes as part of a routine system for clinical analysis.
A procedure for automatic classification of G-banded human chromosomes has been implemented on a semiautomated system for routine clinical analysis. Chromosomes represented by their density profiles are described by so-called weighted density distributions (WDDs) by application of a number of weighting functions and classified by a parametric discriminant analysis. During 16 mo of routine use of the system, 2,794 metaphases (127,925 chromosomes) from amniotic fluid have been karyotyped by the system with an error rate of 8-9%. This corresponds to 4-5 errors per metaphase. These errors can immediately be corrected by the operator on a displayed karyogram with a light pen.
D000653:Amniotic Fluid / Q000032:analysis; D001322:Autoanalysis; D001773:Blood Cells / Q000032:analysis; D002871:Chromosome Banding / Q000379:methods; D002877:Chromosomes, Human / Q000145:classification*; D003201:Computers; D003951:Diagnostic Errors; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008433:Mathematics; D008677:Metaphase