[Method for contaminating test objects with coagulated blood].
A special method to contaminate test objects with blood is described. It is characterized by the use of coagulable blood and its coagulation when adhering to the test objects. Above all, the method is suitable for the microbiological evaluation of the efficacy of agents and methods for the disinfection of surfaces to which coagulated blood will adhere (e.g. instruments). Immediately upon sampling, the blood is heparinized (Liquemin 500 from Hoffmann-La Roche AG at a ratio of 0.1 ml per 100 ml blood). Until use, the coagulable blood is stored at 0 degrees C. For the contamination of test objects, the test organisms are added to the blood (at 0 degrees C), either by mixing the blood with a small amount of a suspension of the test organism or by centrifuging this suspension and dispersing the sedimented organisms in the blood. To start coagulation, Protamin 1000 (Hoffmann-La Roche) is added at a ratio of 0.15 ml per 10 ml blood. Immediately afterwards, the test objects are contaminated with the coagulating blood. Until complete coagulation of the blood, taking ca. 15 min, the test objects are stored in a humid chamber at ca. 20 degrees C.
D001777:Blood Coagulation; D001800:Blood Specimen Collection; D003428:Cross Infection / Q000517:prevention & control*; D004866:Equipment Contamination; D006493:Heparin; D006801:Humans; D008722:Methods
D006493:Heparin
Eine Methode zur Kontamination von Testobjekten mit gerinnendem Blut.