[The role of methods using immunofluorescence (platelet suspension immunofluorescence test and granulocyte immunofluorescence test) in the diagnosis of autoimmune thrombocytopenia and neutropenia].
The authors examined 46 patients with autoimmune thrombocytopenic Purpura and 17 patients with autoimmune neutropenia, with the direct and indirect immunofluorescence method. They intended to verify the sensibility of such methods and to determine the class of immunoglobulins. In the PTA the direct PSIFT was positive in 26 of the 46 examined patients, while the indirect in 15 cases. In the GPA the direct GIFT was positive in 9 of the 17 patients and the indirect in 6 cases. By using FITC-labelled monospecific anti-immunoglobulin reagents, in the PTA we have found IgG in the 57.7% of the patients, IgM in the 11.5% and IgG + IgM in 30.8%; in the GPA we have found IgG in the 66% of the cases, IgM in 11% and IgG + IgM in 23%. These methods have shown a good reliability and sensibility, but also delicacy.
D000380:Agranulocytosis / Q000175:diagnosis*; D001327:Autoimmune Diseases / Q000097:blood / Q000150:complications*; D001792:Blood Platelets; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006098:Granulocytes; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D009503:Neutropenia / Q000175:diagnosis* / Q000209:etiology; D013861:Thiocyanates; D013921:Thrombocytopenia / Q000175:diagnosis* / Q000209:etiology
D005452:Fluoresceins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate
Ruolo dei metodi che impiegano l'immunofluorescenza (PSIFT e GIFT) nella diagnosi delle trombocitopenie e neutropenie autoimmuni.