Galactosyltransferase activities in cultured urinary bladder tumor cells.
After demonstrating that three bladder cancer cell lines (human bladder transitional cell carcinoma, MGH-U1; rat bladder transitional cell carcinoma, RBTCC; Nara rat bladder epidermal carcinoma, NBT-II) had galactosyltransferase (GT) activity in their cell surfaces, we investigated the effect of increasing cell density on the activity of this enzyme. All three cell lines responded to increased cell density by increased activity of cell-surface GT towards endogenous acceptor. By the use of exogenous acceptor, we showed that in the two transitional cell carcinoma lines (human and rat), the increased activity was probably caused by increased levels of endogenous acceptor rather than enzyme. In the rat bladder epidermal carcinoma line, on the other hand, increased GT activity seemed to be the result of increased levels of the enzyme. These conclusions were supported by the increased shedding of GT into the medium with increasing cell density in case of the epidermal carcinoma cells, but not the two transitional cell carcinoma lines. Total cell-associated GT activity would indicate that, in contrast to the two transitional cell carcinoma lines, the bladder epidermal carcinoma cells may have an increased rate of synthesis of GT as confluence is approached.
D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005700:Galactosyltransferases / Q000378:metabolism*; D006801:Humans; D008985:Monensin / Q000494:pharmacology; D051381:Rats; D013499:Surface Properties; D014357:Trypsin / Q000378:metabolism; D014530:Uridine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000201:enzymology*
D014530:Uridine Diphosphate; C033179:UDP dialdehyde; D008985:Monensin; D005700:Galactosyltransferases; D014357:Trypsin