Use of fluorescent standards in protein transfer and immunoblotting. Accurate estimation of the molecular weight of immunoreactive species.
Fluorescent protein molecular weight markers have been used to standardize the electrotransfer and subsequent immunostaining of proteins separated by sodium dodecyl sulfate-polyacrylamide electrophoresis. The method permits accurate estimation of the molecular weight of immunoreactive species despite shrinkage or swelling which may occur during transfer or staining.
D003101:Collodion; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007120:Immunochemistry; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D012015:Reference Standards; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins; D013861:Thiocyanates; D003101:Collodion; D016650:Fluorescein-5-isothiocyanate