title: Effects of aphidicolin on premature condensation of sperm chromosomes in fertilized sea urchin eggs.
abstract: Unfertilized Strongylocentrotus purpuratus eggs may be treated with ammonia to initiate maternal DNA replication and the maternal cell cycle. When these eggs are polyspermically fertilized 75 min after the beginning of ammonia treatment, the nuclei of the fertilizing spermatozoa undergo premature chromosome condensation (PCC) in an apparent attempt to conform to the advanced maternal cell cycle. PCC is inhibited if maternal DNA replication is blocked by exposing the eggs to aphidicolin but will proceed if this exposure begins after replication is complete. Additionally, PCC will proceed in ammonia-activated, polyspermically fertilized anucleate merogons in the continuous presence of aphidicolin. These results suggest that the direct inhibitory effect of aphidicolin may well be limited to the replication of DNA and that the unreplicated maternal nucleus itself exerts negative control over the development of chromosome-condensing conditions in the maternal cytoplasm.
mesh_terms: D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D016590:Aphidicolin; D002875:Chromosomes; D004261:DNA Replication / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D005260:Female; D008297:Male; D012617:Sea Urchins / Q000235:genetics*; D013094:Spermatozoa / Q000187:drug effects*; D015053:Zygote / Q000187:drug effects*
chemical_list: D004224:Diterpenes; D016590:Aphidicolin; D000641:Ammonia