Covalent binding of aflatoxin B1 to liver DNA in rats pretreated with ethanol.
Male Fischer F-344 rats were given ethanol in the drinking water and/or by single oral administration. Following this, the animals received p.o. 100 ng/kg of the hepatocarcinogen [3H]aflatoxin B1 (AFB1). 24 h later, the level of DNA-bound AFB1 was determined in the liver and was found not to be affected by any type of ethanol pretreatment. A cocarcinogenic effect of ethanol in the liver is therefore unlikely to be due to an effect on the metabolic activation inactivation processes governing the formation of DNA-binding AFB1 metabolites.
D000284:Administration, Oral; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism*; D000818:Animals; D003043:Cocarcinogenesis; D004247:DNA / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000139:chemically induced; D051381:Rats; D011916:Rats, Inbred F344
D000348:Aflatoxins; D000431:Ethanol; D004247:DNA; D016604:Aflatoxin B1