title: New evidence for the inhibition by harringtonine in the formation of the first peptide bond in protein synthesis.
abstract: New evidence for the inhibition by harringtonine in the formation of the first peptide bond in protein synthesis was obtained by means of experiments in the cell-free system. The antitumour drug strongly blocked dipeptide synthesis in the system without elongation factor G and GTP. Furthermore, it inhibited N-Acetyl-phenylalanyl-puromycin formation from N-Acetyl-phenylalanyl-tRNA, puromycin, and ribosomes.
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000595:Amino Acid Sequence / Q000187:drug effects; D002474:Cell-Free System; D004917:Erythromycin / Q000494:pharmacology; D006248:Harringtonines / Q000494:pharmacology*; D008239:Lysine / Q000096:biosynthesis*; D011487:Protein Conformation / Q000187:drug effects; D011691:Puromycin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D012270:Ribosomes / Q000378:metabolism
chemical_list: D000470:Alkaloids; D006248:Harringtonines; C046712:N-acetylphenylalanylpuromycin; D011691:Puromycin; D004917:Erythromycin; D008239:Lysine