Phospholipid methylation in rabbit aorta.
The formation of phosphatidylcholine by successive methylations of phosphatidylethanolamine using S-adenosylmethionine as the methyl donor was studied in homogenates of rabbit aorta. Addition of phosphatidylmonomethylethanolamine and phosphatidyldimethylethanolamine, but not phosphatidylethanolamine, stimulated methyltransferase activity and this activity was further stimulated when the phospholipids were dispersed in taurocholate prior to addition to the assay system. No incorporation of radiolabel into sphingomyelin or lysolecithin was detected indicating minimal metabolism of newly formed phosphatidylcholine. The majority of methyltransferase activity was detected in the high-speed pellet of the aortic homogenate; however, since activity was also detected in the high-speed supernatant, the low-speed supernatant preparation was used as the source of enzyme. Methyltransferase activity was characterized in cultured arterial smooth muscle cells using methionine as the radiolabeled precursor. The major product formed was phosphatidylcholine. No difference in enzyme activity was seen as a function of the length of time that cells were in culture or anatomic location of the aortic explant used as a source of cells. Treatment of the cells with cycloheximide did not affect methyltransferase activity. The ability of catecholamine agonists and vasoactive peptides to influence methyltransferase activity was investigated both in the cell-free preparation and in cultured cells. These compounds did not appear to alter methyltransferase activity in rabbit aortic smooth muscle cells.
D000818:Animals; D001011:Aorta / Q000378:metabolism; D002395:Catecholamines / Q000494:pharmacology; D002474:Cell-Free System; D066298:In Vitro Techniques; D008297:Male; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D050918:Phosphatidylethanolamine N-Methyltransferase; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011817:Rabbits
D002395:Catecholamines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C032000:phosphatidyl-N-monomethylaminoethanol; C026508:phosphatidyldimethylethanolamine; D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase