title: [The role of histones H3 and H1 in the formation of thymine-lysine cross-links during UV-irradiation of deoxyribonucleoprotein].
abstract: The effect of UV light (lambda = 254 nm) on calf thymus DNP at low ionic strengths was studied. It was found that at the irradiation doses used the protein in the DNA-protein complex increases as the irradiation dose rises. Thermal treatment and acid hydrolysis resulted in a predominant release of histones H3 and H1 from the complex. Data from liquid high performance chromatography, amino acid analysis, thin-layer chromatography point to the induction by UV-light of a thymine-lysine bond, whose formation involves DNA thymines and histone lysine residues, predominantly H3 and H1 fractions.
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D004268:DNA-Binding Proteins / Q000032:analysis / Q000528:radiation effects*; D003852:Deoxyribonucleoproteins / Q000032:analysis / Q000528:radiation effects*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis / Q000528:radiation effects*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008239:Lysine / Q000032:analysis / Q000528:radiation effects; D010777:Photochemistry; D013941:Thymine / Q000032:analysis / Q000528:radiation effects; D013950:Thymus Gland; D014466:Ultraviolet Rays
chemical_list: D004268:DNA-Binding Proteins; D003852:Deoxyribonucleoproteins; D006657:Histones; D008239:Lysine; D013941:Thymine
vernacular_title: Uchastie gistonov H3 i H1 v obrazovanii timin-lizinovoĭ shivki pri UF-obluchenii deoksiribonukleoproteida.