Sugar transport in Trypanosoma brucei: a suitable kinetic probe.
A transport assay has been developed for use in the investigation of 1-deoxy-D-glucose uptake in trypanosomes. 1-Deoxy-D-glucose has high affinity for the trypanosome sugar transport system (net influx Km = 4.03 +/- 0.42 mM; V = 0.052 +/- 0.005 mM X s-1. D-Glucose oxidation is competitively inhibited by 1-deoxy-D-glucose. However, we show that 1-deoxy-D-glucose is not a substrate for metabolism and that the competition occurs because of interaction at the transport system. D-Glucose competitively inhibits 1-deoxy-D-glucose influx.
D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D050260:Carbohydrate Metabolism; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007700:Kinetics; D014346:Trypanosoma brucei brucei / Q000378:metabolism*
C006584:1,5-anhydroglucitol; D003847:Deoxyglucose; D006593:Hexokinase; D005947:Glucose