Lipid peroxidation decreases the rotational mobility of cytochrome P-450 in rat liver microsomes.
Phenobarbital-induced rat liver microsomes were subjected to NADPH- and Fe2+-catalyzed lipid peroxidation. The formation of approx. 95 nmol malondialdehyde/mg protein during 18 min peroxidation at 37 degrees C was observed. Membrane rigidity measured by means of the steady-state fluorescence anisotropy rs of diphenylhexatriene increased in parallel with the malondialdehyde formation. Both the amount of malondialdehyde and rs remained constant thereafter during incubation of the peroxidized membranes for 2 h. The aminopyrine demethylase activity decreased by about 60% upon lipid peroxidation for 18 min, whereas no significant loss of benzphetamine demethylase activity within the same time range was observed. A time-dependent formation of protein complexes of high molecular weight, comprising most of the microsomal polypeptides, upon lipid peroxidation was observed in SDS-polyacrylamide gel electrophoresis. The effect of microsomal lipid peroxidation on protein-protein interactions was examined by measuring the rotational mobility of intact cytochrome P-450. Rotational diffusion was measured by observing the decay of flash-induced absorption anisotropy r(t) of the P-450 X CO complex. Analysis was based on a 'rotation-about-membrane normal' model with the equation r(t) = r1exp(-t/phi 1) + r2exp(-t/phi 2). In control microsomes, two classes (rapid and slow) of rotating populations of cytochrome P-450 were observed with phi 1 approximately equal to 150 microseconds, fraction r1/(r1 + r2) approximately equal to 40% and phi 2 approximately equal to 2 ms, fraction r2/(r1 + r2) approximately equal to 60%. A relatively small decrease in the rotational mobility of P-450 was observed by a 18-min lipid peroxidation, while a subsequent incubation of peroxidized microsomes for 2 h at 37 degrees C resulted in a dramatic immobilization of P-450 by the increase of both r2/(r1 + r2) approximately equal to 75% and phi 2 approximately equal to 10-25 ms. The decrease in the P-450 mobility during 18-min lipid peroxidation would be due to the rigidification of the lipid bilayer. However, because the lipid fluidity remained unchanged thereafter, the significant immobilization of P-450 by the subsequent 2-h incubation is deduced to be due to formation of protein aggregates.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System; D004591:Electrophoresis, Polyacrylamide Gel; D005454:Fluorescence Polarization; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000096:biosynthesis; D008433:Mathematics; D008560:Membrane Fluidity / Q000187:drug effects; D008862:Microsomes, Liver / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D013997:Time Factors
D008054:Lipid Peroxides; D008315:Malondialdehyde; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase