Detection of mucosal and serum antibodies specific for the capsular polysaccharide of Haemophilus influenzae type b by enzyme-linked immunosorbent assay.
A sensitive enzyme-linked immunosorbent assay (ELISA) with rough-surfaced glass beads used as the solid phase was developed for detection of IgG, IgM, and IgA antibodies to Haemophilus influenzae type b capsular polysaccharide (HITB-CP). A successful method for indirect coating of glass bead surfaces with HITB-CP, and parameters affecting the specificity and sensitivity of the assay are described. This ELISA system proved to be 100 times as sensitive as the standard indirect fluorescent-antibody assay. The assay was applied to the measurement of antibodies to HITB-CP in serum and nasal secretions and proved to be a useful tool in the evaluation of immunological response to HITB infection.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006193:Haemophilus influenzae / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000032:analysis; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007223:Infant; D008297:Male; D009297:Nasal Mucosa / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011817:Rabbits
D000907:Antibodies, Bacterial; D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains; D011135:Polysaccharides, Bacterial; D006735:Horseradish Peroxidase