A human-mouse chimeric immunoglobulin gene with a human variable region is expressed in mouse myeloma cells.
We have constructed and obtained expression of a chimeric human-mouse immunoglobulin gene after transfection into mouse myeloma cells. A human VDJH gene segment was joined to a mouse C kappa gene in the plasmid vector pSV2-gpt, and the construct was transfected into J558L cells by protoplast fusion. Analyses of six transformants by RIA and SDS-PAGE indicated that the chimeric protein was synthesized in large amounts in five. A kappa-specific transcript was observed by Northern blot analysis. Four out of five clones were stable producers of this chimeric chain over a period of 10 mo.
D000818:Animals; D002459:Cell Fusion; D002678:Chimera; D002999:Clone Cells / Q000378:metabolism; D005796:Genes; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000378:metabolism; D010954:Plasmacytoma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D009194:Myeloma Proteins; D012333:RNA, Messenger