title: Immunocytochemical demonstration of both carbonic anhydrase isoenzyme II and glial fibrillary acidic protein in some immature rat glial cells in primary culture.
abstract: In glial cell cultures from newborn rat hemispheres we have demonstrated by immunocytochemistry the presence of carbonic anhydrase (CAII) and glial fibrillary acidic protein (GFAP) in the same cell. These double-stained cells are detected between day 5 and day 13 after seeding. These cells could be morphologically identified as oligodendroblasts. At 5 days in culture about 10% of the cells containing CAII also contained GFAP. At 11 days only 2% preserved this property. After 13 days in culture GFAP was only localized in typical astrocytes and CAII in oligodendroglial-like cells. The presumption that the immature glial cells, which contained specific markers of astrocytes and oligodendrocytes, became in our culture conditions essentially oligodendroglial cells is discussed.
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology; D001921:Brain / Q000166:cytology; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007527:Isoenzymes; D002256:Carbonic Anhydrases