[Prostaglandins and applications in clinical biology].
Pure acetylcholine esterase from Electrophorus Electricus has been covalently coupled to different prostaglandins or thromboxanes. The corresponding conjugates have been tested using microtiter plates with 96 wells coated with a second antibody. The minimal detectable concentrations were 10 pg/ml. We suggest that this procedure which has been extended to non icosanoid molecules may serve as a general technique for enzyme immunoassay.
D015121:6-Ketoprostaglandin F1 alpha / Q000031:analogs & derivatives / Q000652:urine; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002675:Child, Preschool; D002849:Chromatography, Gas; D015237:Dinoprost; D015232:Dinoprostone; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn; D013058:Mass Spectrometry; D011453:Prostaglandins / Q000032:analysis* / Q000378:metabolism; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D013929:Thromboxane B2 / Q000032:analysis; D013931:Thromboxanes / Q000032:analysis; D014652:Vascular Diseases / Q000175:diagnosis / Q000652:urine
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D013931:Thromboxanes; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C028290:2,3-dinor-6-ketoprostaglandin F1alpha; D015237:Dinoprost; D015232:Dinoprostone
Prostaglandines et applications en biologie clinique.