Isolation and characterization of a genomic DDD mouse interleukin-3 gene.
A chromosomal DNA segment containing the entire gene for interleukin-3 (IL-3) was isolated from DDD mouse erythroleukemia cells, and its gene organization and nucleotide sequence were determined. Enhancer-like sequences in the second intron and G + C-rich sequence in the 5'-flanking region may play a role in the regulation of tissue-specific and inducible gene expression. Southern blot analyses revealed that constitutively IL-3-producing WEHI-3 cells have the rearranged IL-3 gene, and that genomic DNAs prepared from the adult and fetal mouse liver have the same organization of the IL-3 gene. No IL-3 gene transcript was detected in the mouse erythroleukemia cells by Northern blot analysis.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D007377:Interleukin-3; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000276:immunology; D008222:Lymphokines / Q000235:genetics*; D051379:Mice
D007377:Interleukin-3; D008222:Lymphokines; D004247:DNA