Molecular cloning and nucleotide sequence of the streptavidin gene.
Using synthetic oligonucleotides as probes we have cloned the streptavidin gene from a genomic library of Streptomyces avidinii. Nucleotide sequence analysis indicated that a 2 Kb DNA-fragment contained the entire coding region, a signal peptide region and the 3' and 5' flanking regions of the gene. The deduced amino acid sequence shows several interrupted blocks of homology with the amino acid sequence of chicken egg-white avidin. Analysis of the secondary structure suggests a high content of beta-structure in both proteins and considerable overall structural similarity between them.
D000595:Amino Acid Sequence; D001360:Avidin; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D011487:Protein Conformation; D019809:Streptavidin; D013302:Streptomyces / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001360:Avidin; D019809:Streptavidin