[Various properties of purified hepatoredoxin from bovine liver mitochondria].
Hepatoredoxin purified to homogeneity from bovine liver mitochondria has been characterized for the first time in terms of its most important physico-chemical properties. The protein was found to contain in its active center a [2Fe-2S] cluster and has in the oxidized state an absorption maxima at 280, 320, 415 and 455 nm. The spectrophotometric index of purity, A415/A280 of the homogeneous native preparation is 0.84; extinction coefficient, epsilon 415, is 9800 M-1cm-1. The Mr of hepatoredoxin as evidenced by data from SDS gel electrophoresis is 12 500 Da; pI is 4.2. Hepatoredoxin is necessary for the reconstitution of the C27-steroid hydroxylase activity and can be substituted for by a related protein, adrenodoxin. All the above parameters as well as the circular dichroism spectra, immunochemical properties and sequence of the initial five N-terminal amino acids of hepatoredoxin and adrenodoxin are either coincident or very close. At the same time, the amino acid composition of these ferredoxins, apart from some common features, has individual peculiarities.
D000302:Adrenal Cortex / Q000032:analysis; D000325:Adrenodoxin / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000032:analysis* / Q000302:isolation & purification; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D007668:Kidney / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D008930:Mitochondria, Liver / Q000032:analysis* / Q000201:enzymology; D011487:Protein Conformation; D013250:Steroid Hydroxylases / Q000032:analysis
D000596:Amino Acids; D005288:Ferredoxins; C041734:hepatoredoxin; D000325:Adrenodoxin; D013250:Steroid Hydroxylases
Nekotorye svoĭstva ochishchennogo gepatoredoksina iz mitokhondriĭ pecheni byka.