Phospholipid metabolism and prolactin secretion in vitro.
The possible mechanisms by which phospholipid metabolism may be involved in the biochemical events underlying pituitary hormone secretion in basal and stimulated conditions were examined. Particular emphasis was given to the role of changes in the turnover of specific membrane phospholipids, the polyphosphoinositides, in the stimulatory effect of TRH and neurotensin on prolactin release in vitro. Finally, some comments on the involvement of arachidonate and/or its metabolites in the mechanisms of release of the hormone have been reported. In this respect, the possibility that a specific diacylglycerol lipase may represent a link between the 'phosphatidylinositol effect' and the production of arachidonate from mammotroph membranal phospholipids was examined using the rather selective inhibitor of diacylglycerol lipase RHC80267.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002417:Cattle; D066298:In Vitro Techniques; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism; D010716:Phosphatidylinositols / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
D001095:Arachidonic Acids; D010716:Phosphatidylinositols; D010743:Phospholipids; D016718:Arachidonic Acid; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin; D008071:Lipoprotein Lipase