Production and partial characterization of monoclonal hybridoma antibodies to Mycobacterium tuberculosis.
The production and partial characterization of monoclonal hybridoma antibodies against Mycobacterium tuberculosis is described. 30 clones have been produced. Their reactivity towards M. tuberculosis antigens have been tested in immunoblotting from SDS-PAGE, in crossed immunoelectrophoresis and in ELISA. The Mab's separated into four reactivity groups, one of which may be relevant in assays for antigen specific for M. tuberculosis.
D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D002478:Cells, Cultured; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology*; D007123:Immunoelectrophoresis, Two-Dimensional; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010209:Paper
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D003101:Collodion