Synthesis and expression of the native RNase T1 gene and several mutant genes.
RNase T1 gene and several mutant genes were constructed by joining of chemically synthesized deoxyoligonucleotides. These genes were inserted into an expression vector and expressed as fused protein in E. coli. RNase T1 and its mutant enzymes were liberated by cyanogen bromide treatment and their activities were measured.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004722:Endoribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005813:Genes, Synthetic; D005822:Genetic Vectors; D009154:Mutation; D006163:Ribonuclease T1 / Q000235:genetics* / Q000302:isolation & purification; D014158:Transcription, Genetic
D004722:Endoribonucleases; D006163:Ribonuclease T1