T cell involvement in production of tumor necrosis factor: reconstitution experiments with nude mice.
In order to investigate the role of T cells in the production of tumor necrosis factor (TNF), a reconstitution experiment was performed with nude mice (Balb/c, nu/nu). The results obtained were as follows: 1) The cytotoxic activity of tumor necrosis serum (TNS) from Balb/c, nu/nu mice treated with Propionibacterium acnes-LPS was 1/22 of that from Balb/c, nu/+ mice. 2) TNF activity increased 14 times in reconstituted nude mice as compared to Balb/c, nu/nu mice. 3) The production of the cytotoxic activity per cell was investigated using T cell and macrophage fractions separated from the spleens of both Balb/c, nu/nu and Balb/c, nu/+ mice treated with P. acnes as a priming agent. Elicitation with LPS was done in vitro. Release of cytotoxic activity into the culture medium was observed in the macrophage fraction, but not in the T cell fraction. However, no significant species difference was found. 4) With P. acnes treatment, the population of macrophages in the spleens from Balb/c, nu/+ mice increased 25.5 times, whereas that from Balb/c, nu/nu mice only increased 6.8 times. The above results suggest that the mechanism of the incremental effect of T cells on TNF production was due to the promotion of macrophage proliferation during the priming period after injection of P. acnes.
D000818:Animals; D000921:Antibody-Producing Cells / Q000032:analysis / Q000276:immunology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006023:Glycoproteins / Q000096:biosynthesis / Q000276:immunology*; D007114:Immunization; D007518:Isoantibodies / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011425:Propionibacterium acnes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D007518:Isoantibodies; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C035678:anti-Thy antibody