title: V lambda 2 rearranges with all functional J lambda segments in the mouse.
abstract: We have analyzed 210 lambda-producing hybridomas derived from lipopolysaccharide-stimulated spleen cells from a single kappa-suppressed mouse. All were classified as lambda 1, lambda 2 or lambda 3 with the exception of four unusual lines. Two of these were due to V lambda 2 J lambda 1 and the other two to V lambda 2 J lambda 3 rearrangements. The lines were clonally independent since the point of VJ recombination in each one was different. Southern blot analysis of the V lambda 2 C lambda 1-producing lines showed no evidence for an inversion. Under the assumption of a simple deletion model of rearrangement these findings place the V lambda 2 cluster upstream of the V lambda 1 cluster oriented in the same direction.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006825:Hybridomas / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000096:biosynthesis; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis*; D007146:Immunoglobulin lambda-Chains / Q000096:biosynthesis*; D007526:Isoelectric Point; D051379:Mice; D011995:Recombination, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007144:Immunoglobulin J-Chains; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains