[Isolation and purification of lactoperoxidase from cow's milk].
A procedure is described for isolation and purification of lactoperoxidase from cow milk. The procedure involved the following steps: isolation of casein from milk, sorption of lactoperoxidase on CM-Sephadex, concentration of the eluate using ultrafiltration, salting out with ammonium sulfate; isoelectric focusing was carried out in the borate-polyol system. Highly purified, active preparation of lactoperoxidase was obtained within a relatively short period by means of the procedure, where the available reagents were used.
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007525:Isoelectric Focusing; D007784:Lactoperoxidase / Q000302:isolation & purification*; D008892:Milk / Q000201:enzymology*; D010544:Peroxidases / Q000302:isolation & purification*
D007784:Lactoperoxidase; D010544:Peroxidases
Vydelenie i ochistka laktoperoksidazy iz korov'ego moloka.