title: Immunological responses to protein, carbohydrate and lipid fractions of Nocardia asteroides in mice.
abstract: The protein, polysaccharide and phospholipid constituents of Nocardia asteroides have been partially purified and their immunogenicity studied in mice. Humoral and cellular immune responses were demonstrated against a crude cytoplasmic protein fraction (CPF). Two fractions of CPF were prepared on a Sephadex G-200 column; a high mol. wt fraction, fraction-1(F1) was capable of eliciting both types of immune responses, whereas fraction-2(F2) behaved more like a hapten. Phosphatides elicited only humoral responses whereas polysaccharides were non-immunogenic.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002464:Cell Migration Inhibition; D003378:Counterimmunoelectrophoresis; D005260:Female; D007114:Immunization; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007962:Leukocytes / Q000276:immunology; D008297:Male; D051379:Mice; D009616:Nocardia asteroides / Q000276:immunology*; D010743:Phospholipids / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D010743:Phospholipids; D011135:Polysaccharides, Bacterial