title: High-performance liquid chromatographic molecular weight determination of allergen extracts. Examination of the influence of the column material on allergenic activity and allergen patterns.
abstract: To investigate whether the column material employed in size-exclusion high-performance liquid chromatography (HPLC) influences the allergenic activity and antigen/allergen patterns of allergen extracts, the molecular weights of a mite and a birch pollen allergen extract were determined using a Bio-Sil TSK 250 column with a guard column. The allergenic activities were measured by RAST inhibition and the antigen/allergen patterns were determined by crossed (radio)immunoelectrophoresis. The original extracts and the corresponding eluates after HPLC runs showed the same allergenic activity and the same number of antigen/allergen precipitation lines. Only slight differences in the peak heights of some precipitates were observed.
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D008970:Molecular Weight; D011058:Pollen / Q000032:analysis; D011852:Radioallergosorbent Test
chemical_list: D000485:Allergens; D007202:Indicators and Reagents