Separate regulatory elements for the heat-inducible and ovarian expression of the Drosophila hsp26 gene.
We have constructed and transformed into the D. melanogaster germ line a series of hsp26 deletion variants. We show that the region -728 to +14 contains all of the cis-acting sequences necessary for the developmental and heat-inducible transcription of hsp26. Sequences upstream of -341 are not required for heat-inducible expression but are required for ovarian expression. Conversely, sequences between -351 and -53 are unnecessary for ovarian expression but are needed for maximal heat-inducible expression. We conclude that most, or all, of the cis-acting regulatory sequences required for hsp26 gene expression during oogenesis are physically separable from those required for heat-inducible expression. These results suggest that different trans-acting factors bind at different regulatory sites in the 5'-flanking region of the gene, thus activating its transcription.
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D005809:Genes, Regulator; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D009866:Oogenesis; D010053:Ovary / Q000378:metabolism; D011679:Pupa / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D006360:Heat-Shock Proteins; D011994:Recombinant Proteins