Effects of ONO-3122 (an enhancer of PGH2 production) and OKY-1581 (an inhibitor of TXA2 production) on the vasopressin-induced water flow in the toad bladder.
The effects of 1-iodo-3-aminomethyl-5,6,7,8-tetrahydro-2-naphthol (ONO-3122) which increases endogenous PGH2, and sodium (E)-2-methyl-3-[4-3-pyridylmethyl)phenyl]-2-methylpropenoate (OKY-1581) which inhibits thromboxane A2 synthesis, on vasopressin-induced osmotic water flow in the bladder of the toad, Bufo bufo japonicus, were examined. ONO-3122 significantly inhibited the vasopressin-induced water flow at a concentration of 1 X 10(-4) M. OKY-1581 inhibited the vasopressin-induced water flow at 1 X 10(-6) M, but enhanced it at 1 X 10(-4) M. These results suggest that ONO-3122 indirectly inhibits the vasopressin-induced osmotic water flow in the toad bladder; that is, ONO-3122 causes an increase in the conversion of arachidonic acid into PGH2. These results also suggest that OKY-1581 at a low concentration suppresses the vasopressin-induced water flow due to inhibition of cyclooxygenase activity. Both ONO-3122 and OKY-1581 provide a useful means for studying the action of prostaglandins.
D000179:Acrylates / Q000494:pharmacology*; D000818:Animals; D001834:Body Water / Q000378:metabolism*; D002023:Bufo bufo; D066298:In Vitro Techniques; D008689:Methacrylates / Q000494:pharmacology*; D009284:Naphthols / Q000494:pharmacology*; D009994:Osmolar Concentration; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D011450:Prostaglandin Endoperoxides, Synthetic / Q000096:biosynthesis; D044262:Prostaglandin H2; D011463:Prostaglandins H / Q000096:biosynthesis; D013928:Thromboxane A2 / Q000096:biosynthesis; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors*; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism; D014667:Vasopressins / Q000494:pharmacology*
D000179:Acrylates; D008689:Methacrylates; D009284:Naphthols; D011450:Prostaglandin Endoperoxides, Synthetic; D011463:Prostaglandins H; D014667:Vasopressins; D044262:Prostaglandin H2; D013928:Thromboxane A2; C031517:2-methyl-3-(4-(3-pyridinylmethyl)phenyl)-2-propenoic acid; C035374:1-iodo-3-aminomethyl-5,6,7,8-tetrahydro-2-naphthol; D010088:Oxidoreductases; D013930:Thromboxane-A Synthase