Selectivity of acyl transfer between phospholipids: arachidonoyl transacylase in dog heart membranes.
Dog heart microsomes catalyze the transfer of acyl groups from the sn-2 position of exogenous phosphatidylcholine to 1-acyl lysophosphatidylethanolamine. Approximately equal amounts of free fatty acids are produced as well. The reaction exhibits a pH optimum of 7.5-8.5 and does not require Ca2+ or other divalent cations. The reaction proceeds in the absence of exogenous coenzyme A but acyl transfer is enhanced by its addition. The transacylase exhibits a strong preference for arachidonate over linoleate and thus may be involved in the maintenance of the high amounts of arachidonate found in microsomal ethanolamine phospholipids.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D004285:Dogs; D066298:In Vitro Techniques; D008246:Lysophospholipids; D008563:Membrane Lipids / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*
D001095:Arachidonic Acids; D008246:Lysophospholipids; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C008301:lysophosphatidylethanolamine; D016718:Arachidonic Acid; D000217:Acyltransferases; C039766:arachidonyl transacylase