Heparin and the solubilization of asymmetric acetylcholinesterase.
Heparin solubilizes asymmetric acetylcholinesterase, from chick skeletal muscle and retina, as a 24 S complex which is quantitatively converted to conventional asymmetric molecular forms of the enzyme (A12 and A8, either class I or class II) upon exposure to high salt. The simultaneous presence of salt and heparin in the homogenization medium selectively prevents, however, the release of class II A-forms in both muscle and retina. Heparin may generally act by displacing native proteoglycans involved in the attachment of the enzyme tail to the extracellular matrix, or its neural equivalent, being in turn removed by salt to yield typical asymmetric enzyme forms. Heparin would also appear to displace some other molecules specifically involved in the EDTA-sensitive attachment of class II tailed forms, this effect being antagonized by salt.
D000110:Acetylcholinesterase; D000818:Animals; D002645:Chickens; D006493:Heparin; D009132:Muscles / Q000201:enzymology; D012160:Retina / Q000201:enzymology; D012995:Solubility
D006493:Heparin; D000110:Acetylcholinesterase