Functional analysis of macrophage hybridomas. II. Antibody-independent tumor cytotoxicity and its dissociation from IL-1 production and Ia expression.
Cloned macrophage hybridoma cells derived from fusions between splenic adherent cells of CKB mice and the drug-marked P388D1 cell line were studied to further understand macrophage-mediated tumoricidal activity at the clonal level. Most of the macrophage hybridomas were specifically activated by a combination of lymphokine and lipopolysaccharide (LPS), but were not activated by either agent alone. There is no apparent correlation between macrophage-mediated tumor cytotoxicity and the ability of LPS to induce interleukin 1 (IL-1) secretion or of concanavalin A (Con A) supernatants containing interferon-gamma to increase H-2 and Ia expression. Thus, one hybridoma clone which was unable to kill tumor cells was still sensitive to LPS and lymphokine activation by the latter criterion.
D000818:Animals; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D007375:Interleukin-1 / Q000096:biosynthesis*; D007941:Leukemia P388 / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H
D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008222:Lymphokines