NMR, CD and IR spectroscopies of a tridecanucleotide containing a no-base residue: coexistence of B and Z conformations.
The synthesis of the tridecadeoxynucleotide d(CGm5CGCGxACATGT), where x is the 1-cyano-2-deoxy-beta-D-erythropentofuranose, is described. The NMR, IR, CD studies at various salt concentrations and temperatures of this oligomer show that the B and Z conformations are simultaneously present in the same short DNA fragment. A single apurinic residue is sufficient for the coexistence of the B and Z helices on this oligomer.
D001483:Base Sequence; D002942:Circular Dichroism / Q000379:methods; D003855:Deoxyribose / Q000031:analogs & derivatives*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D009994:Osmolar Concentration; D013055:Spectrophotometry, Infrared / Q000379:methods; D013816:Thermodynamics
D009838:Oligodeoxyribonucleotides; C047933:1-cyano-2-deoxyribose; D003855:Deoxyribose