Practical "stat" method for radioimmunoassay.
A prompt and reasonably precise radioimmunoassay result is often urgently required, but it is difficult to satisfy such requests without running a complete standard curve, even when only a single sample is to be assayed. To obtain a greater constancy in the response of the RIA system, I propose a method for use of RIA in a "stat" (i.e., urgent) method: maximum binding and nonspecific binding for RIA data processing are "calculated" from response indexes obtained in a previous assay run with the same reagents and from the binding values of two or three standards processed together with a single sample. These calculated values are then used to calculate the response index for the single sample, which is directly interpolated in the calibration curve of the previous non-stat assay. I checked the validity of this stat method for eight analytes, comparing a total of 552 analytical results obtained by the conventional and the stat method. This method is shown to give adequately precise results for use in clinical emergencies.
D006801:Humans; D007202:Indicators and Reagents; D008433:Mathematics; D010672:Phenytoin / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods*; D013997:Time Factors
D007202:Indicators and Reagents; D010672:Phenytoin