The analysis of an anti-fluorescein response at limit dilution: the effect of lymphokine preparations on the apparent specificity.
The specificity of cytotoxic T cells in an anti-fluorescein response has been analysed at limit dilution. According to the ability to discriminate between fluoresceinated and non-fluoresceinated targets, the apparent specificity of CTL depends on the quality and quantity of the lymphokine preparations used to support the response. An acid-labile component of the factor preparations contributes to the generation of non-specific CTL. No cytotoxicity against NK-sensitive target cells was detected in the 'non-specific' component of the responses.
D000143:Acids / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013861:Thiocyanates / Q000276:immunology*; D013997:Time Factors
D000143:Acids; D005452:Fluoresceins; D007376:Interleukin-2; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate