New evidence for the inhibition by harringtonine in the formation of the first peptide bond in protein synthesis.
New evidence for the inhibition by harringtonine in the formation of the first peptide bond in protein synthesis was obtained by means of experiments in the cell-free system. The antitumour drug strongly blocked dipeptide synthesis in the system without elongation factor G and GTP. Furthermore, it inhibited N-Acetyl-phenylalanyl-puromycin formation from N-Acetyl-phenylalanyl-tRNA, puromycin, and ribosomes.
D000470:Alkaloids / Q000494:pharmacology*; D000595:Amino Acid Sequence / Q000187:drug effects; D002474:Cell-Free System; D004917:Erythromycin / Q000494:pharmacology; D006248:Harringtonines / Q000494:pharmacology*; D008239:Lysine / Q000096:biosynthesis*; D011487:Protein Conformation / Q000187:drug effects; D011691:Puromycin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D012270:Ribosomes / Q000378:metabolism
D000470:Alkaloids; D006248:Harringtonines; C046712:N-acetylphenylalanylpuromycin; D011691:Puromycin; D004917:Erythromycin; D008239:Lysine