Facilitated transport of 3-O-methyl-D-glucose in human polymorphonuclear leukocytes.
Transport of the nonmetabolizable hexose analogue 3-O-methyl-D-glucose (30MG) was measured in human polymorphonuclear leukocytes at 37 degrees C, pH 7.4. 3OMG at very low concentration (0.05 mM) equilibrated with the intracellular water with a rate constant of about 0.08 s-1. Transport of 3OMG in the presence of 20 microM cytochalasin B and transport of L-glucose were insignificant. Countertransport of 14C-labelled 3OMG was demonstrated. Exchange of 3OMG between the extracellular and intracellular water showed saturation with a Km of about 4 mM. Thus, the transport of 3OMG is mediated almost exclusively by facilitated diffusion.
D001692:Biological Transport; D003571:Cytochalasin B / Q000494:pharmacology; D004058:Diffusion; D006801:Humans; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D008757:Methylglucosides; D008759:Methylglycosides; D014867:Water; D003571:Cytochalasin B