Metabolic activation of hepatocarcinogens in chronic hepatitis B.
S9 fraction pools of liver biopsy samples, collected from 129 patients in two consecutive studies, were comparatively assayed for their ability to activate aflatoxin B1 (AFB1) and a tryptophan pyrolysate product (Trp-P-2) in a miniaturized Salmonella mutagenicity test system. Metabolic activation was not affected to a significant extent by most of the monitored variability factors, such as sex, alcohol, cigarette smoking and liver histology (minimal changes, chronic persistent (CPH) or active (CAH) hepatitis, CAH steatosis, or cirrhosis). Conversely, a significant enhancement of activation was observed for AFB1 in cases of mild CAH and especially for Trp-P-2 in hepatitis B virus carriers, irrespective of their histologic diagnosis.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism; D001711:Biotransformation; D002243:Carbolines / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism* / Q000633:toxicity; D006509:Hepatitis B / Q000378:metabolism*; D006521:Hepatitis, Chronic / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D012486:Salmonella typhimurium / Q000187:drug effects
D000348:Aflatoxins; D002243:Carbolines; D002273:Carcinogens; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D016604:Aflatoxin B1