Ca++ localization in boar spermatozoa by the pyroantimonate technique and X-ray microanalysis.
Intracellular, loosely bound Ca++ has been localized electron microscopically in freshly ejaculated boar spermatozoa by in situ precipitation with potassium antimonate. Ca++ was identified as the cation precipitated by testing the EGTA-sensitivity of the precipitates and by X-ray microprobe analysis. The data obtained revealed that the outer acrosomal membrane is the preferential site for Ca++ precipitation in the sperm head.
D000818:Animals; D000965:Antimony; D002118:Calcium / Q000032:analysis*; D004577:Electron Probe Microanalysis / Q000379:methods; D008297:Male; D008854:Microscopy, Electron; D013094:Spermatozoa / Q000648:ultrastructure*; D013552:Swine
C009133:pyroantimonate; D000965:Antimony; D002118:Calcium