title: Comparison of American MicroScan dry and frozen microdilution trays.
abstract: This study compared the American MicroScan frozen microdilution tray and dehydrated microdilution tray. A total of 153 gram-negative and 106 gram-positive isolates were tested concurrently on both tray types and read visually. Very major and major antimicrobial susceptibility discrepancies amounted to 1.0% of all results, and minor discrepancies amounted to 0.4% of all results. The biochemical discrepancy rate for gram-negative organisms was 1.3%, resulting in no identification errors either at the genus or the species level. Gram-positive identification was not evaluated, as the formulation of biochemicals was changed after this evaluation was completed. Overall, the results with the dry tray were acceptable, providing a suitable alternative to the frozen tray.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects / Q000378:metabolism; D013293:Enterococcus faecalis / Q000145:classification / Q000187:drug effects / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005615:Freezing; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000187:drug effects / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D011933:Reagent Kits, Diagnostic