Cytolytic T cell hybridomas. II. Interleukin 2-independent cytolytic variants.
Interleukin 2 (IL2)-dependent cytolytic hybrids usually loose IL2 dependence and cytolytic activity concomittantly. Here we describe an exceptional IL2-independent variant which lost IL2 receptor but retained cytolytic activity. When the IL2-independent cytolytic hybrid cells were fused with IL2-dependent cytolytic clones which express IL2 receptors constitutively, IL2-dependent hybrids were obtained. This suggests that the expression of IL2 receptors can interfere with IL2-independent growth mechanisms.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion; D002459:Cell Fusion; D002460:Cell Line; D003470:Culture Media; D014644:Genetic Variation; D006825:Hybridomas / Q000276:immunology*; D019271:Hypoxanthine; D007042:Hypoxanthines; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010042:Ouabain; D011971:Receptors, Immunologic / Q000276:immunology; D015375:Receptors, Interleukin-2; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007042:Hypoxanthines; D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D019271:Hypoxanthine; D010042:Ouabain