Determination of calmodulin by competitive binding assay.
Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a tool to readily probe changes in calmodulin levels in cells and tissues as a function of pathophysiologic state.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002147:Calmodulin / Q000032:analysis*; D002417:Cattle; D003599:Cytoskeleton / Q000032:analysis; D006801:Humans; D007118:Immunoassay; D007457:Iodine Radioisotopes; D008297:Male; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D002147:Calmodulin; D007457:Iodine Radioisotopes