Efficient purification of human plasma beta 2-microglobulin from the haemodialysate of a patient with chronic renal failure by use of high-performance liquid chromatography.
Human plasma beta 2-microglobulin was isolated in a good yield (more than 80%) from the haemodialysate (blood ultrafiltrate) of a patient with chronic renal failure. The isolation procedure consisted of Sephadex G-100 gel filtration and two steps of high-performance liquid chromatography: reversed-phase high-performance liquid chromatography and gel permeation chromatography. The purified beta 2-microglobulin was homogeneous by sodium dodecyl sulphate polyacrylamide gel electrophoresis and two-dimensional electrophoresis.
D001798:Blood Proteins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D006435:Renal Dialysis; D012967:Sodium Dodecyl Sulfate; D014462:Ultrafiltration; D001613:beta 2-Microglobulin / Q000302:isolation & purification*
D001798:Blood Proteins; D001613:beta 2-Microglobulin; D012967:Sodium Dodecyl Sulfate