Activation of the gamma 1 gene by lipopolysaccharide and T cell-derived lymphokines containing a B cell differentiation factor for IgG1 (BCDF gamma).
By using both pulse labeling of nascent RNA chains and lactoperoxidase-catalyzed cell surface radioiodination, we examined both the de novo synthesis of mRNA for gamma-chains and the expression of membrane IgG (mIgG) on cells which had been stimulated with LPS plus a T cell supernatant (SN) containing a B cell differentiation factor for IgG1 (BCDF gamma). Our results show that neither nascent mRNA for gamma 1 chains nor mIgG1 can be detected in B lymphocytes until they have been stimulated by both LPS and BCDF gamma-containing T cell SN, and suggest that cell surface expression and secretion of IgG1 are coordinately controlled.
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D005260:Female; D005802:Genes, MHC Class II; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis / Q000145:classification; D013601:T-Lymphocytes / Q000378:metabolism*; D014158:Transcription, Genetic
D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell