The routine measurement of platelet volume: a comparison of aperture-impedance and flow cytometric systems.
The effect of dipotassium ethylenediaminetetraaceticacid (EDTA) on the platelet count and mean volume (MPV) was evaluated using two routine measurement systems, a Coulter S Plus Phase 1 (S+) and a Technicon H6000 (H6000). In normal subjects (n = 29) MPV increased by 17% during 39 h storage in EDTA when measured by the S+. In contrast MPV decreased by 22% when measured by the H6000. MPV differences of up to 40% were observed between the two systems. Concomitant platelet counts, in both systems, changed by less than 4%. Using the anticoagulant sodium citrate and prostaglandin E1 (Na-citrate/PGE1 there were no significant changes in MPV measured by the S+ during 7 h storage, although a linear decrease in platelet count was observed. A decrease in H6000 MPV was observed whether the blood was stored in EDTA or Na-citrate/PGE1. Methodology, anticoagulation and storage time all influence MPV. Until these determinants are standardized the clinical value of MPV cannot be assessed.
D000527:Alprostadil; D000925:Anticoagulants / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D001811:Blood Volume Determination / Q000379:methods*; D002951:Citrates; D019343:Citric Acid; D004492:Edetic Acid / Q000494:pharmacology; D005434:Flow Cytometry; D006403:Hematologic Tests; D006801:Humans
D000925:Anticoagulants; D002951:Citrates; D019343:Citric Acid; D004492:Edetic Acid; D000527:Alprostadil