[Characteristics of the capsular antigen preparations isolated from a broth culture of Yersinia pestis strain EV].
The physico-chemical and immunobiological study of the preparation of capsular antigen, isolated by a high-yield method from the culture fluid of Y. pestis grown in nutrient broth at 37 degrees C and practically free from the admixture of polysaccharide and nucleic acids, has been made. The study has revealed that this capsular antigen is protein with a molecular weight of 1.65-1.69 X 10(6) D and sedimentation constant equal to 13.4S; its viscosity is 220 ml/g and its isoelectric point is at pH 4.1. The preparations have been found to possess immunogenic and allergenic properties and to be capable of sensitizing formulated, tannin-treated sheep red blood cells to plague antiserum.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D007114:Immunization; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins
Kharakteristika preparatov kapsul'nogo antigena, vydelennykh iz bul'onnoĭ kul'tury shtamma EV Yersinia pestis.