Further evaluation of lectin affinity purified glycoprotein (GP90) in the enzyme linked immunosorbent assay (ELISA) for diagnosis of Trypanosoma cruzi infection.
Sera from 143 patients considered to be infected with Trypanosoma cruzi on the basis of epidemiological, clinical and standard serological evidence gave positive results in the enzyme-linked immunosorbent assay (ELISA) using a lectin affinity purified 90,000 molecular weight glycoprotein (GP90) antigen preparation. Levels of antibody did not discriminate between clinically classified groups of patients in the chronic phase of infection. The GP90 preparation was found to be heterogeneous.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D014355:Chagas Disease / Q000175:diagnosis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D015800:Protozoan Proteins; D014349:Trypanosoma cruzi / Q000276:immunology; D014643:Variant Surface Glycoproteins, Trypanosoma
D000906:Antibodies; D000941:Antigens; D000953:Antigens, Protozoan; D006023:Glycoproteins; D015800:Protozoan Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; C044186:glycoprotein GP90, Trypanosoma cruzi