Purification and some properties of a low molecular weight trypsin inhibitor from acute pancreatitis urine.
Urinary trypsin inhibitors (UTIs) from the urine of a patient with acute pancreatitis consisted of three forms with different molecular weights. These were highly purified by ammonium sulfate precipitation, Sephadex G-75, SP-Sephadex C-25 and trypsin-Sepharose 4B column chromatography. The lowest molecular weight of UTIs was estimated to be 6,200 daltons. Moreover, five residues of N-terminal amino acids and a C-terminal amino acid were the same as those of pancreatic secretory trypsin inhibitor.
D000208:Acute Disease; D000596:Amino Acids / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D008970:Molecular Weight; D010195:Pancreatitis / Q000652:urine*; D014361:Trypsin Inhibitors / Q000032:analysis / Q000302:isolation & purification / Q000652:urine*
D000596:Amino Acids; D014361:Trypsin Inhibitors