title: Physical conditions during batch culture of hybridomas in laboratory scale stirred tank reactors.
abstract: Due to the nature of animal cell suspension culture, physical conditions during growth such as pH, pO2, pCO2, agitation and aeration are inter-related and can exhibit individual or combined effects on cells. Evaluation of their influence on hybridoma growth and monoclonal antibody production can only be achieved by the use of well-instrumented stirred tank reactors. To determine the effect of the various physical conditions, mouse hybridomas, excreting antibody specific for human IgG, have been cultured in a 2 litre mechanically agitated round bottomed glass vessel, equipped with facilities to monitor and control these parameters during cell growth. Batch culture has been carried out at 36 degrees C in medium supplemented with 5% foetal calf serum (FCS). Clones have also been grown successfully with 2% FCS additions and in serum free medium, after careful adaptation periods. At least two clones have been assessed for comparative purposes.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002245:Carbon Dioxide; D002455:Cell Division; D003584:Cytological Techniques; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D002245:Carbon Dioxide