An improved method for the demonstration of fibrin in biopsies of rabbit skin homografts.
Tissues embedded in resin are convenient for routine use when the presence or absence of fibrin in them is to be confirmed using the electron microscope. To visualize fibrin using the light microscope, sections (1.0-2.0 micron) from such specimens should be stained with Methylene Blue-Azure II-Basic Fuchsin (MBBF). Staining with MBBF is more controllable than with other methods and it requires only two short staining steps. Compared with Giemsa, MBBF provides a polychromatic, as opposed to a monochromatic end-result, sharply contrasting fibrin (blue) against collagen (pink-violet).
D000818:Animals; D001391:Azo Compounds; D001399:Azure Stains; D001706:Biopsy; D004396:Coloring Agents; D005337:Fibrin / Q000032:analysis*; D005557:Formaldehyde; D005976:Glutaral; D008297:Male; D008751:Methylene Blue; D011817:Rabbits; D012867:Skin / Q000032:analysis*; D014184:Transplantation, Homologous
D001391:Azo Compounds; D001399:Azure Stains; D004396:Coloring Agents; D005557:Formaldehyde; D005337:Fibrin; C042643:Martius scarlet blue trichrome; D005976:Glutaral; D008751:Methylene Blue