Use of temperature-sensitive liposomes in the selective delivery of methotrexate and cis-platinum analogues to murine bladder tumor.
This report describes the release of methotrexate and cis-platinum analogues JM8 and JM9 from phase transition liposomes. Large unilamellar liposomes were able to quickly release the antineoplastic agents employed during in vitro tests. Methotrexate exhibited a 73 per cent release in one second. The cis-platinum analogues JM8 and JM9 were slower, showing 43.5 per cent and 33.5 per cent release at two seconds. In vivo experiments utilizing MBT-2 transitional cell carcinoma-bearing mice were conducted. Methotrexate delivered by the liposome complex showed an 8.4-fold increase in heated tumor uptake when compared to unheated tumors.
D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000188:drug therapy*; D002945:Cisplatin / Q000378:metabolism / Q000627:therapeutic use*; D008081:Liposomes / Q000378:metabolism; D008727:Methotrexate / Q000378:metabolism / Q000627:therapeutic use*; D051379:Mice; D008809:Mice, Inbred C3H; D013696:Temperature; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy*
D008081:Liposomes; D002945:Cisplatin; D008727:Methotrexate