title: Distribution and processing of the polymeric immunoglobulin receptor in the rat hepatocyte: morphological and biochemical characterization of subcellular fractions.
abstract: The transepithelial transport of polymeric immunoglobulins is an essential process in the mucosal immune system. Transport across the epithelial cells of mucous or exocrine glands is affected by an integral membrane glycoprotein receptor known as membrane secretory component (SCm) or as polymeric immunoglobulin receptor (pIgR). This receptor binds polymeric immunoglobulins at the basolateral cell surface and mediates their transcellular translocation and their release from the apical plasma membrane into external secretions. Release depends on cleavage of the membrane-anchoring domain of the receptor, resulting in liberation of polymeric immunoglobulin bound to the ectoplasmic domain of the receptor (secreted SC or SCs) into extracellular secretions. Using a monoclonal antibody directed against the cytoplasmic tail of the receptor and a polyclonal antibody directed against the secreted ectoplasmic domain, we have combined cell fractionation and Western blotting techniques to examine the fate of these receptor domains in the hepatocyte. In this study, we characterize biochemically and morphologically the various subcellular components separated by our fractionation scheme, and correlate this with biochemical analysis of the receptor in each fraction.
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D006056:Golgi Apparatus / Q000032:analysis; D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments / Q000032:analysis*; D008099:Liver / Q000032:analysis* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D011971:Receptors, Immunologic / Q000032:analysis*; D012635:Secretory Component / Q000032:analysis*
chemical_list: D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D011971:Receptors, Immunologic; D012635:Secretory Component; C034639:polymeric IgA