Influence of five different anticoagulants on human blood monocyte isolation and functional activities.
The recovery of blood monocytes after centrifugation with metrizoate/polysucrose was high with EDTA (97.0%) and heparin (91.6%) as anticoagulants, moderate with EGTA and citrate, but low with oxalate (52.3%; P less than 0.05 compared with heparin). There were no differences in viability, spontaneous migration, chemotaxis, or phagocytosis and killing of Candida albicans by the monocytes isolated from blood anticoagulated with heparin, citrate, oxalate, EDTA, or EGTA. However, heparin in concentrations above 20 IU/ml decreased the spontaneous migration and chemotaxis. It is concluded, that EDTA and heparin below 20 IU/ml are the most satisfactory anticoagulants for the functional study of blood monocytes isolated by metrizoate/polysucrose density centrifugation.
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008297:Male; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D010070:Oxalates / Q000494:pharmacology; D019815:Oxalic Acid; D010587:Phagocytosis / Q000187:drug effects
D000925:Anticoagulants; D002951:Citrates; D010070:Oxalates; D019343:Citric Acid; D004533:Egtazic Acid; D006493:Heparin; D019815:Oxalic Acid; D004492:Edetic Acid