High-performance liquid chromatographic separation of lens crystallins and their subunits.
A high-performance liquid chromatography (HPLC) method for the separation of lens crystallins has been developed which utilizes molecular sieve HPLC. Also described is a rapid one-step separation of the lens crystallin subunits using a reversed-phase C4 column.
D000375:Aging; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003459:Crystallins / Q000302:isolation & purification*; D007908:Lens, Crystalline / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D003459:Crystallins