The genotype and phenotype of T cell and non-T, non-B acute lymphoblastic leukemia.
We used Southern blot analysis to investigate the T cell receptor and immunoglobulin (Ig) genes in tumor DNA derived from 13 patients with acute lymphoblastic leukemia (ALL). Rearrangement of both alleles of the T cell receptor beta chain gene was demonstrated in the three individuals with T cell ALL, while the Ig genes remained in germline configuration. In contrast, in nine of ten patients with non-T, non-B ALL (seven common ALL antigen [CALLA]-positive), Ig genes were rearranged while the T cell receptor genes were intact: the noteworthy exception was a CALLA-positive individual in whom one allele of the T cell receptor was rearranged and in whom a minor rearranged Ig band was detected as well. This exception indicates that CALLA-positive non-T, non-B ALL includes proliferations that have undergone an initial genetic step toward T cell differentiation. With probes now available for both the Ig and T cell receptor genes, analysis of genomic tumor DNA is useful in all variants of ALL.
D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005802:Genes, MHC Class II; D005838:Genotype; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics*; D010641:Phenotype; D013601:T-Lymphocytes
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D004273:DNA, Neoplasm