Insulin-like growth factor-I action on hypothyroid rat pituitary cells: suppression of triiodothyronine-induced growth hormone secretion and messenger ribonucleic acid levels.
T3 stimulates in vitro GH secretion, whereas somatomedins have been shown to suppress GH. We, therefore, tested the effects of a recombinant human insulin-like growth factor analog (IGF-I; Thr 59) on T3-induced GH secretion. Pituitary monolayer cultures were derived from 4- to 7-week surgically thyroidectomized male rats and grown in hypothyroid medium stripped of T3 and T4. Hypothyroid pituitary cell monolayers secreted low levels of GH (200 ng/ml X 72 h). GH secretion was stimulated (185% of T3-free controls) by the addition of T3 (0.1 nM) during 48 h of incubation. IGF-I (up to 13 nM) did not alter basal GH secretion, but inhibited the induction of GH by T3. IGF-I (0.65 nM) suppressed the 3.5-fold induction of GH by 0.25 nM T3 by 35% (p less than 0.001), whereas maximal (68%) GH suppression was seen with 6.5 nM IGF-I (p less than 0.001). The inhibition of T3-induced GH by IGF-I occurred after 24 h. By 72 h, IGF-I (3.25 nM) suppressed GH stimulation by up to 1 nM T3 [213 +/- 12 (+/- SE) vs. 88 +/- 8 ng GH/10(4) cells]. Insulin, epidermal growth factor, and fibroblast growth factor did not alter the T3-induced GH secretion. GH mRNA was measured by agarose gel blot hybridization analysis and nitrocellulose dot blot hybridization of cytoplasmic RNA with rat GH [32P]cDNA. T3 (0.25 nM) stimulated the hybridization of immobilized pituitary cell RNA extracts, indicating an almost 3-fold increase in rat (r) GH mRNA levels; IGF-I (3.25 nM) suppressed the T3-induced rGH mRNA content of pituitary cells from thyroidectomized rats by 50% during 72 h of incubation. Maximal suppression of rGH mRNA induced by 0.25 nM T3 was achieved with 6.5 nM IGF-I (35% of T3-treated cells). These results show that IGF-I does not alter low basal GH secretion in pituitary cells from hypothyroid rats. IGF-I does, however, block the T3-induction of GH secretion and suppresses GH mRNA levels stimulated by T3. As T3 is known to directly stimulate GH gene transcription, IGF-I may suppress T3 action on GH at a posttranscriptional site.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D007037:Hypothyroidism / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D010902:Pituitary Gland / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013002:Somatomedins / Q000494:pharmacology*; D013965:Thyroidectomy; D014284:Triiodothyronine / Q000494:pharmacology*
D012333:RNA, Messenger; D013002:Somatomedins; D014284:Triiodothyronine; D013006:Growth Hormone