Growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin B.
Vibrio alginolyticus, Streptomyces griseus, Arthrobacter G12, Bacillus sp. and Cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. Arthrobacter G12, Bacillus sp. and Cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. All bacteria produced extracellular proteases and all bacteria except Arthrobacter G12 produced extracellular chitinases. Growth of Cytophaga sp. on colloidal chitin was paralleled by the accumulated chitinase activity in the culture filtrate, and growth of Cytophaga sp. and Arthrobacter G12 on cell walls of Geotrichum candidum and cell walls of Candida pseudotropicalis was paralleled by the accumulation of laminarinase activity in the culture filtrate, but little or no extracellular chitinase activity was observed in these cultures. Mycolases purified from the culture filtrates of Cytophaga sp. grown on colloidal chitin on cell walls of C. pseudotropicalis potentiated the antifungal activity of amphotericin B.
D000666:Amphotericin B / Q000494:pharmacology*; D001173:Arthrobacter / Q000254:growth & development*; D001407:Bacillus / Q000254:growth & development*; D002175:Candida / Q000187:drug effects*; D002473:Cell Wall; D002686:Chitin; D002688:Chitinases / Q000378:metabolism*; D003589:Cytophaga / Q000254:growth & development*; D005848:Geotrichum / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D003904:Mitosporic Fungi / Q000187:drug effects*; D013045:Species Specificity; D013305:Streptomyces griseus / Q000254:growth & development*; D014733:Vibrio / Q000254:growth & development*
D002686:Chitin; D000666:Amphotericin B; D002688:Chitinases