title: Studies on residual antibacterials in foods. IV. Simultaneous determination of penicillin G, penicillin V and ampicillin in milk by high-performance liquid chromatography.
abstract: A rapid and simple method for the simultaneous determination of penicillin G (PCG), penicillin V (PCV) and ampicillin (ABPC) in milk is described. The retention behaviour of these beta-lactam antibiotics in reversed-phase liquid chromatography with mobile phases containing sodium alkylsulphonate was studied. Good separations were obtained with methanol-water-0.2 M phosphate buffer (pH 4.0) (5:13:2) containing 11 mM sodium 1-heptanesulphonate and a LiChrosorb RP-18 column. The sample was pre-treated with a Sep-Pak C18 cartridge. The peaks corresponding to each beta-lactam antibiotics can be confirmed with the treatment using penicillinase. The recoveries from milk fortified with sodium PCG, potassium PCV and ABCP at levels of 0.5 and 0.1 micrograms/g each were generally better than 87% and the relative standard deviations were 1.17-4.98%. The detection limits corresponded to 0.03 microgram/g of these beta-lactam antibiotics in milk.
mesh_terms: D000667:Ampicillin / Q000032:analysis; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008892:Milk / Q000032:analysis*; D010400:Penicillin G / Q000032:analysis; D010404:Penicillin V / Q000032:analysis; D010405:Penicillinase
chemical_list: D000890:Anti-Infective Agents; D007202:Indicators and Reagents; D000667:Ampicillin; D010405:Penicillinase; D010400:Penicillin G; D010404:Penicillin V