Enhanced production of human interferon-gamma by the use of a combination of inducers--phytohemagglutinin, picibanil and tumor promoting agent.
Phytohemagglutinin (PHA), picibanil (OK432) and tumor promoting agent (TPA) were tested in various combinations for optimal induction of human interferon-gamma (HuIFN-gamma). It was found that the use of a mixture of all 3 inducers resulted in IFN production 2-3 times higher than either PHA (10 micrograms/ml) in combination with TPA (5 ng/ml) or picibanil (10 micrograms/ml) alone. The IFN was produced by T lymphocytes and could be neutralized by specific IFN-gamma antiserum. It was pH 2.0 labile and species specific.
D001688:Biological Products / Q000494:pharmacology*; D004347:Drug Interactions; D006801:Humans; D007369:Interferon Inducers; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D010704:Phorbols / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D010844:Picibanil / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D001688:Biological Products; D007369:Interferon Inducers; D010704:Phorbols; D010835:Phytohemagglutinins; D010844:Picibanil; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate