Studies on assay of estradiol binding proteins by cytochemical and immunocytochemical techniques in human breast tumors and other tissues.
Estradiol binding proteins in frozen sections of human breast tumors, ovarian carcinomas, hypernephromas, rat uteri and cow udders were demonstrated by fluorescent labeled estradiol bovine serum albumin conjugate (cytochemical or direct method). The specificity of the binding of estradiol conjugate to tissue sections was tried to approach by addition of various steroid or non-steroidal competitors. The estradiol conjugate binding sites were practically not present in sections of human spleen, cross muscle and colon muscle.
D000328:Adult; D000368:Aged; D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis*; D002417:Cattle; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D007680:Kidney Neoplasms / Q000032:analysis; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000032:analysis; D051381:Rats; D012738:Sex Hormone-Binding Globulin; D014599:Uterus / Q000032:analysis
D002352:Carrier Proteins; D012738:Sex Hormone-Binding Globulin