Study of fluorescein glucuronide. I. Isolation from urine.
A method for isolating fluorescein glucuronide from the urine using silica gel chromatography and preparative high-performance liquid chromatography is described. The product obtained was free from significant fluorescent impurity on thin-layer chromatography and analytical high-performance liquid chromatography.
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D005452:Fluoresceins / Q000302:isolation & purification / Q000652:urine*; D008297:Male; D011817:Rabbits
D005452:Fluoresceins; C041357:fluorescein glucuronide