A sum of 14C-putrescine metabolites as a measure of DAO activity. Column chromatography assay.
The rapid ion-exchange chromatographic method was developed for the separation of 14C-putrescine from its metabolites arising from diamine oxidase (DAO, E.C. 1.4.3.6) catalysed reaction. The assay is linear for putrescine conversion with both, time and enzyme concentration. With this method many samples could be analysed simultaneously.
D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis*; D000818:Animals; D002250:Carbon Radioisotopes; D002852:Chromatography, Ion Exchange; D005260:Female; D006168:Guinea Pigs; D011700:Putrescine / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006631:Amine Oxidase (Copper-Containing); D011700:Putrescine