title: Bromo-A23187: a nonfluorescent calcium ionophore for use with fluorescent probes.
abstract: 4-Bromo-A23187, a halogenated analog of the widely studied divalent cation ionophore A23187, is a nonfluorescent Ca2+ ionophore suitable for use in the calibration of cytoplasmic free Ca2+ by fluorescent probes. Br-A23187 is shown to saturate Ca2+ sites in quin-2-loaded rat thymic lymphocytes in a manner essentially identical to ionomycin.
mesh_terms: D000634:Aminoquinolines; D000818:Animals; D000001:Calcimycin / Q000031:analogs & derivatives*; D002118:Calcium / Q000032:analysis*; D007476:Ionophores; D008081:Liposomes; D010713:Phosphatidylcholines; D051381:Rats; D013050:Spectrometry, Fluorescence; D013601:T-Lymphocytes / Q000032:analysis
chemical_list: D000634:Aminoquinolines; D007476:Ionophores; D008081:Liposomes; D010713:Phosphatidylcholines; D000001:Calcimycin; C033170:4-bromo-A-23187; C035738:Quin2-acetoxymethyl ester; D002118:Calcium