The amino-acid sequence of the variable region of a carbohydrate-containing amyloid fibril protein EPS (immunoglobulin light chain, type lambda).
The amino-acid sequence of the variable region of a carbohydrate-containing amyloid fibril protein EPS of immunoglobulin lambda light chain origin has been elucidated. The protein was isolated from the liver of a patient (EPS) with an immunocyte dyscrasia of the IgM type. The molecular mass of this protein was found to be about 20 kDa including an oligosaccharide chain linked to it. The amino-acid sequence determination involved automatic Edman degradation of polypeptides obtained after cleaving the protein with BNPS-skatole, trypsin and thermolysin. The proposed sequence of the variable region of the protein showed that it may be assigned to the V lambda I subgroup. A tryptic and a thermolysinolytic peptide both containing the carbohydrate were isolated and characterized, and the localization of an oligosaccharide chain linked to asparagine was established.
D000595:Amino Acid Sequence; D000682:Amyloid / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007135:Immunoglobulin Variable Region / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis*; D012862:Skatole / Q000031:analogs & derivatives / Q000494:pharmacology; D013820:Thermolysin / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D000682:Amyloid; D002241:Carbohydrates; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; C046640:amyloid fibril protein EPS; C005904:BNPS-skatole; D012862:Skatole; D014357:Trypsin; D013820:Thermolysin