Intermediate filament heterogeneity in normal and hypercholesterolemic rabbit vascular smooth muscle cells.
We have examined the intermediate filament (IF) protein content of vascular smooth muscle (SM) cells from several arteries and veins in rabbits and quantitated the changes which occur in SM cell expression of these proteins in response to cholesterol feeding. Cells from control rabbit arteries expressed 30% of their IF protein as desmin, while veins expressed 50% as desmin. During development of diet-induced atherosclerosis, morphological changes in arterial SM cells in the intima correlate with changes in IF expression. There is a significant increase in total IF protein content, vimentin increased differentially in thoracic aorta and desmin in pulmonary artery. In abdominal aorta both increase equally. Cholesterol feeding also resulted in changes in the expression of subspecies of desmin, vimentin, and actin in the thoracic arch. Although cholesterol feeding did not produce obvious morphological changes in the veins examined, venous SM IF protein expression was also altered. In the vena cava of cholesterol-fed rabbits there was an increase in vimentin expression without the parallel increase in desmin that occurred in the arterial system. These studies show that cholesterol feeding of rabbits induces measurable changes in the amounts of IF proteins in both arterial atherosclerotic lesions and venous SM cells.
D000199:Actins / Q000032:analysis; D000818:Animals; D001012:Aorta, Abdominal / Q000032:analysis; D001013:Aorta, Thoracic / Q000032:analysis; D001161:Arteriosclerosis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D003893:Desmin / Q000032:analysis; D006937:Hypercholesterolemia / Q000378:metabolism* / Q000473:pathology; D007381:Intermediate Filament Proteins / Q000032:analysis*; D007382:Intermediate Filaments / Q000032:analysis / Q000648:ultrastructure; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000032:analysis* / Q000648:ultrastructure; D011651:Pulmonary Artery / Q000032:analysis; D011817:Rabbits; D014684:Venae Cavae / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D000199:Actins; D002791:Cholesterol, Dietary; D003893:Desmin; D007381:Intermediate Filament Proteins; D014746:Vimentin