title: Characterization of monoclonal antibodies against human protein C specific for the calcium ion-induced conformation or for the activation peptide region.
abstract: Three monoclonal antibodies have been produced that are specific for the activation peptide region in human protein C. These antibodies inhibited the activation of protein C by thrombin and by the thrombin-thrombomodulin complex. A fourth monoclonal antibody specifically recognized the Ca2+-stabilized conformation in protein C. This antibody bound both intact protein C and protein C from which the gamma-carboxyglutamic acid-containing region had been removed by limited proteolysis. These results indicate that this antibody recognizes the conformation in protein C stabilized by Ca2+ bound to the single binding site that is independent of gamma-carboxyglutamic acid.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001665:Binding Sites; D002118:Calcium / Q000502:physiology*; D004492:Edetic Acid / Q000494:pharmacology; D006023:Glycoproteins / Q000276:immunology*; D007114:Immunization; D051379:Mice; D011486:Protein C; D011487:Protein Conformation; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011486:Protein C; D004492:Edetic Acid; D002118:Calcium