Approaches to the study of mammalian cellular phospholipases.
The purpose of this article is to present some problems encountered in the study of cellular phospholipases and some approaches used in answering these problems. Examples of a number of phospholipases that differ in their origin and positional specificity are given. Consideration is given to enzyme purification, mode of action, and interaction with substrate and function in situ.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002384:Catalysis; D006801:Humans; D007700:Kinetics; D008245:Lysophospholipase / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000378:metabolism
D001095:Arachidonic Acids; D016718:Arachidonic Acid; D010740:Phospholipases; D010741:Phospholipases A; D008245:Lysophospholipase; D010738:Type C Phospholipases; D010739:Phospholipase D