The quantitative determination of neomycin sulphate by a diffusion technique on agar plates by the method of the European Pharmacopoeia, 2nd edition--evaluation of precision and reproducibility of the method.
The medium recommended by the European Pharmacopoeia (EP), 2nd edition, for the microbiological determination of neomycin by the agar diffusion method was tested and compared with the medium recommended by the EP, 1st edition. The tests were carried out in different laboratories. The medium recommended by the EP, 2nd edition, gave greater precision and reproducibility than the previous medium. The possibility of using a reference standard of almost pure neomycin B for both the determination of framycetin and neomycin was evaluated. The results demonstrated that the medium recommended by the EP, 2nd edition, gave better precision and reproducibility. Difficulty in achieving valid assays was practically the same with both media.
D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay / Q000379:methods; D003470:Culture Media; D004058:Diffusion; D005060:Europe; D005069:Evaluation Studies as Topic; D009355:Neomycin / Q000032:analysis*; D010602:Pharmacopoeias as Topic
D003470:Culture Media; D009355:Neomycin