Detection of 2,5-hexanedione in the urine of persons not exposed to n-hexane.
The neurotoxic n-hexane metabolite 2,5-hexanedione was detected by gas chromatography--mass spectrometry in urine samples from human subjects not exposed to n-hexane or related hydrocarbons. Quantitative determinations by multiple ion detection revealed an amount of 2,5-hexanedione ranging from 0.12 to 0.78 mg/l (0.45 +/- 0.20 mg/l; means +/- SD). The amounts of 2,5-hexanedione actually measured in urinary extracts depended on the pH employed for acid hydrolysis of the samples.
D000328:Adult; D004781:Environmental Exposure; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006586:Hexanes / Q000378:metabolism; D006588:Hexanones / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007659:Ketones / Q000652:urine*; D008297:Male; D008875:Middle Aged
D006586:Hexanes; D006588:Hexanones; D007659:Ketones; C026385:n-hexane; C011269:2,5-hexanedione