Bioimmunoassay (BIA) of tissue plasminogen activator (t-PA) and its specific inhibitor (t-PA/INH).
A bioimmunoassay (BIA) for tissue plasminogen activator (t-PA) is described which depends on the binding of t-PA and its inhibitor complexes to an immobilised IgG to t-PA and the subsequent assessment of the bound t-PA using glutamic acid-plasminogen (glu-plgn) and the chromogenic substrate, S-2251. This assay indicated that neither normal plasma nor its euglobulin precipitate contain any measurable free and functionally active t-PA. The BIA was also used to measure the level of the fast acting specific t-PA inhibitor (t-PA/INH) in plasma by assessing the residual t-PA activity in the plasma euglobulin fraction (pH 5.9), following the addition of a known amount of t-PA to the plasma.
D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G; D007700:Kinetics; D010960:Plasminogen Activators / Q000032:analysis* / Q000037:antagonists & inhibitors; D015849:Plasminogen Inactivators; D012712:Serum Globulins / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods
D006023:Glycoproteins; D007074:Immunoglobulin G; D015849:Plasminogen Inactivators; D012712:Serum Globulins; D010960:Plasminogen Activators