title: Peroxidative oxidation of bilirubin during prostaglandin biosynthesis.
abstract: The peroxidative oxidation of bilirubin has been characterized in the ram seminal vesicle microsomal system. The oxidation was monitored by following the loss in absorbance of bilirubin at 440 nm. Bilirubin behaves as a peroxidase substrate for prostaglandin H synthase. The oxidation may be initiated by the addition of arachidonic acid or peroxides to incubations containing ram seminal vesicle microsomes and bilirubin, and is sensitive to inhibition by reduced glutathione. The arachidonate-dependent oxidation, but not the peroxide-initiated case, is inhibited by indomethacin. Similar results were obtained using microsomal preparations from mouse, rat, and pig lungs. Spectral and chromatographic examination of the products of bilirubin oxidation in the ram seminal vesicle system demonstrate that biliverdin is produced in this system by the dehydrogenation of bilirubin, but that this product accounts for only about 15% of the bilirubin consumed. Biliverdin itself is not oxidized in this system. At least three highly polar, fluorescent products also are formed from bilirubin. Though not identified, these polar products differ markedly in chromatographic behavior from the major fluorescent products obtained following the singlet oxygen oxidation or the autoxidation of bilirubin.
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism*; D001664:Biliverdine / Q000096:biosynthesis; D005978:Glutathione / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008168:Lung / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011463:Prostaglandins H / Q000096:biosynthesis; D012669:Seminal Vesicles / Q000378:metabolism*; D012756:Sheep; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
chemical_list: D001095:Arachidonic Acids; D011463:Prostaglandins H; D016718:Arachidonic Acid; D010544:Peroxidases; D011451:Prostaglandin-Endoperoxide Synthases; D005978:Glutathione; D001664:Biliverdine; D001663:Bilirubin; D007213:Indomethacin