Cell calcium of ejaculated rabbit spermatozoa before and following in vitro capacitation.
Ejaculated rabbit spermatozoa were loaded with the calcium-selective fluorescent indicator quin-2. Measurements of trypan blue exclusion indicated that cell viability was not affected by quin-2 loading. The concentration of intracellular free calcium of quin-2 loaded sperm was calculated to be 144 +/- 14 nM. Spermatozoa capacitated in vitro either before or following quin-2 loading had intracellular free calcium levels similar to that of non-capacitated sperm. These studies indicate that the concentration of intracellular free calcium of ejaculated rabbit spermatozoa does not change as a result of in vitro capacitation.
D000177:Acrosome / Q000502:physiology; D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D008297:Male; D011817:Rabbits; D012661:Semen / Q000378:metabolism; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000378:metabolism*
D000634:Aminoquinolines; C035738:Quin2-acetoxymethyl ester; D002118:Calcium