title: 111In-labeled eosinophils: localization of inflammatory lesions and parasitic infections in mice.
abstract: Based upon recent development of practical isolation techniques for eosinophils, labeling and in vivo imaging of eosinophils has been achieved. Isolation of cells was performed utilizing a Percoll density gradient. The eosinophils were subsequently labeled by a modified 111In-oxine method. Migration of eosinophils in response to intradermal ear-pinna injections of SEA (soluble schistosoma egg antigen), S. mansoni eggs, E. coli, and turpentine was followed with gamma-ray camera imaging from 4 to 48 h. Maximal localization, determined by Gamma 11 data processing, occurred by 4-h post-injection of radiolabel. SEA and S. mansoni eggs provided a greater stimulus for localization than E. coli or turpentine. Neutrophils did not preferentially accumulate. Tissue distribution of labeled eosinophils was greatest in the spleen, followed by liver and bone. 111In-labeled-eosinophil scans are sensitive to parasitic infections, although somewhat nonspecific.
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000000981:diagnostic imaging; D002499:Centrifugation, Density Gradient / Q000379:methods; D004804:Eosinophils / Q000166:cytology* / Q000000981:diagnostic imaging; D004927:Escherichia coli Infections / Q000000981:diagnostic imaging*; D006968:Hypersensitivity, Delayed; D007204:Indium / Q000097:blood; D007249:Inflammation; D007700:Kinetics; D008099:Liver / Q000000981:diagnostic imaging; D051379:Mice; D008811:Mice, Inbred DBA; D011868:Radioisotopes; D011877:Radionuclide Imaging; D012555:Schistosomiasis mansoni / Q000000981:diagnostic imaging*; D013154:Spleen / Q000000981:diagnostic imaging; D014018:Tissue Distribution
chemical_list: D011868:Radioisotopes; D007204:Indium