Protection by acyl-carnitines and phenylmethylsulfonyl fluoride of rat heart subjected to ischemia and reperfusion.
Perfusion of rat hearts according to the Langendorff technique with micromolar concentrations of palmitoylcarnitine or millimolar concentrations of phenylmethylsulfonyl fluoride protect the heart from deterioration by reperfusion after total-ischemia. This is based on the retention of the cytosolic enzymes determined (lactate dehydrogenase, glycogen phosphorylase and glycogen synthase) and of myoglobin, as well as on the resumption of contractile activity. Palmitoylcarnitine, like phenylmethylsulfonyl fluoride, could protect through plasma membrane stabilization, since more hydrophilic compounds had no effect.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002331:Carnitine / Q000031:analogs & derivatives*; D002462:Cell Membrane / Q000502:physiology; D003327:Coronary Disease / Q000188:drug therapy* / Q000201:enzymology / Q000503:physiopathology; D003600:Cytosol / Q000201:enzymology; D006006:Glycogen Synthase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009200:Myocardial Contraction; D009211:Myoglobin / Q000378:metabolism; D010172:Palmitoylcarnitine / Q000627:therapeutic use*; D010477:Perfusion; D010664:Phenylmethylsulfonyl Fluoride / Q000627:therapeutic use*; D006005:Phosphorylases / Q000378:metabolism; D051381:Rats; D013450:Sulfones / Q000627:therapeutic use*
D009211:Myoglobin; D013450:Sulfones; D010172:Palmitoylcarnitine; D010664:Phenylmethylsulfonyl Fluoride; D007770:L-Lactate Dehydrogenase; D006005:Phosphorylases; D006006:Glycogen Synthase; D002331:Carnitine; D002118:Calcium