Disposition of 2,4-dinitroaniline in the male F-344 rat.
The disposition and metabolism of 2,4-dinitroaniline was studied in male F-344 rats following oral and i.v. administration. Gastrointestinal absorption was near complete and was not affected by dose (10-90 mumol/kg). Following either oral or i.v. administration, dinitroaniline was rapidly distributed throughout the tissues and showed no marked affinity for any particular tissue. 14C-dinitroaniline was readily cleared by metabolism to at least nine metabolites; radioactivity was excreted primarily in urine (70% dose) and to a lesser extent in faeces (25-30% dose). Clearance of radioactivity from the body was near complete in 3 days. As the whole-body half-life of dinitroaniline derived radioactivity in the rat was less than 3 h and there was no evidence for saturation of any mechanism of absorption, distribution, metabolism or excretion in the dose range studied, 2,4-dinitroaniline appears to have little potential for bioaccumulation in animal tissues. Analysis of urine and bile detected nine metabolites of 2,4-dinitroaniline. The major metabolite was excreted in urine as a sulphate conjugate and in bile as a glucuronide. This metabolite was characterized by combined h.p.l.c./mass spectrometry as 2,4-dinitrophenylhydroxylamine.
D000284:Administration, Oral; D000814:Aniline Compounds / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine; D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D007275:Injections, Intravenous; D008297:Male; D013058:Mass Spectrometry; D008657:Metabolic Clearance Rate; D051381:Rats; D011916:Rats, Inbred F344; D014018:Tissue Distribution
D000814:Aniline Compounds; D002250:Carbon Radioisotopes; C010934:2,4-dinitroaniline