The influence of prolonged clofibrate treatment on the plasma drug concentration and liver NADP-linked enzymes activity.
The clofibric acid (CPIB) concentration in the rat blood plasma during 14 days of treatment with clofibrate and after cessation of the treatment, was measured and compared with the changes of some NADP-linked dehydrogenases activity. CPIB rapidly accumulates in the plasma during the first two days of clofibrate administration reaching a maximum concentration of about 0.6 mM. Then a linear decrease of the drug concentration was observed. The second day after clofibrate treatment had been completed, CPIB could no longer be detected in the plasma. Liver malic enzyme activity increased constantly up to the third day after cessation of the treatment. There was a decrease of activity which reached its control level on the 32nd day of the experiment. The variation patterns of hexose monophosphate shunt dehydrogenases activity and liver weight were similar to the changes of malic enzyme activity.
D000818:Animals; D002994:Clofibrate / Q000097:blood / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000032:analysis; D009249:NADP / Q000032:analysis; D010088:Oxidoreductases / Q000032:analysis*; D010427:Pentose Phosphate Pathway; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D009249:NADP; D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D002994:Clofibrate