Anticomplementary fraction from the poisonous secretion of the paratoid gland of the toad (Bufo marinus paracnemis Lutz).
Fractionation of the poisonous secretion of the toad Bufo marinus paracnemis Lutz, by dialysis and chromatography on QAE-Sephadex, led to the isolation of a fraction which was adsorbed to the ion exchanger. This fraction, when incubated with human serum, yielded an anticomplementary effect that was evaluated by measuring the kinetics of lytic activity on sensitized sheep red cells (classical pathway) and unsensitized rabbit cells (alternative pathway).
D000664:Amphibian Venoms / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002024:Bufo marinus / Q000502:physiology*; D003167:Complement Activation / Q000187:drug effects*; D003170:Complement Pathway, Alternative / Q000187:drug effects; D003171:Complement Pathway, Classical / Q000187:drug effects; D005260:Female; D008297:Male; D010306:Parotid Gland / Q000032:analysis; D013045:Species Specificity
D000664:Amphibian Venoms