Selection of highly transfectable variant from mouse mastocytoma P815.
A tk- cell line derived from mouse mastocytoma P815 was transfected with a plasmid carrying a thymidine kinase gene. The tk+ cells were obtained at a frequency of 10(-6). From some of these tk+ transfectants it was possible to select tk- cells with BrdU so that these cells could be submitted again to transfection with the thymidine kinase gene. By repeating cycles of transfection and tk+ selection followed by reverse selection with BrdU, it was possible to obtain a stable variant having a 100-fold increase in transfection efficiency. This HTR (high transfection) variant shows a high efficiency of transfection (10(-4)) for the neomycin-resistance gene as well as for the thymidine kinase gene.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004351:Drug Resistance; D004587:Electrophoresis, Agar Gel; D005796:Genes; D005819:Genetic Markers; D012515:Mast-Cell Sarcoma / Q000235:genetics; D051379:Mice; D008811:Mice, Inbred DBA; D009355:Neomycin / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012641:Selection, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection
D005819:Genetic Markers; D004247:DNA; D013937:Thymidine Kinase; D009355:Neomycin