A simplified in situ solubilization procedure for the determination of DNA and cell number in tissue cultured mammalian cells.
A rapid and simple procedure for the direct solubilization of DNA from adherent cultured cells, or sedimented cells, has been developed and employed in the measurement of DNA in small numbers of endothelial cells (1 X 10(3).
D000818:Animals; D001769:Blood; D002417:Cattle; D002452:Cell Count / Q000379:methods*; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D004492:Edetic Acid / Q000494:pharmacology; D004727:Endothelium / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D012995:Solubility
D004247:DNA; D004492:Edetic Acid