Thin layer chromatographic method for determination of deoxynivalenol in wheat: collaborative study.
A collaborative study of a rapid method for the determination of deoxynivalenol (DON) in winter wheat was successfully completed. The method involves sample extraction with acetonitrile-water (84 + 16), cleanup using a disposable column of charcoal, Celite, and alumina, and detection by thin layer chromatography after spraying with an aluminum chloride solution. Each of the 15 collaborators analyzed 12 samples, 2 of which were naturally contaminated, and 10 to which DON was added, in duplicate, at levels of 0, 50, 100, 300, and 1000 ng/g. Average recoveries of DON ranged from 78 to 96% with repeatabilities of 30-64% and reproducibilities of 33-87%. The results of the study show that false positives were not a problem and that all of the analysts could detect DON at the 300 ng/g level or higher. The method has been adopted official first action.
D002855:Chromatography, Thin Layer; D005516:Food Microbiology; D007202:Indicators and Reagents; D012717:Sesquiterpenes / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000032:analysis*
D007202:Indicators and Reagents; D012717:Sesquiterpenes; D014255:Trichothecenes; C007262:deoxynivalenol