title: Ornithine decarboxylase inhibitors increase the cellular content of the enzyme: implications for translational regulation.
abstract: Ehrlich ascites tumor cells grown in the presence of inhibitors of ornithine decarboxylase (EC 4.1.1.17) exhibited an elevated content of this enzyme. The increase could not solely be explained by a decrease in the degradation rate of the enzyme. Instead a stimulation of enzyme synthesis, probably mediated via the polyamine-depleting properties of the inhibitors, is suggested. The enhancement of cellular ornithine decarboxylase content was not accompanied by any significant changes in the amount of ornithine decarboxylase mRNA, indicating a regulation at the level of translation.
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA; D000518:Eflornithine; D006207:Half-Life; D009693:Nucleic Acid Hybridization; D009952:Ornithine / Q000031:analogs & derivatives / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D065108:Ornithine Decarboxylase Inhibitors; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D065108:Ornithine Decarboxylase Inhibitors; D012333:RNA, Messenger; C025025:alpha-methylornithine; D004247:DNA; D003513:Cycloheximide; D009952:Ornithine; D009955:Ornithine Decarboxylase; D000518:Eflornithine