title: Sudden volume changes of the lumen of inverted thyroid follicles in suspension cultures. Use in estimation of rates of water flux through thyroid epithelium.
abstract: Inverted thyroid follicles in suspension culture were observed using dark-field illumination and time-lapse video enhancement techniques. The lumens of many follicles undergo episodes of rapid shrinkage followed by relatively slow dilation. The extent of shrinkage ranged from 10 to 40% of the initial diameter. The decrease in lumen diameter is accompanied by loss of luminal fluid. The rate of efflux of fluid amounted to from 1 to 90 microns3/micron2 of luminal surface/min. This was followed after several minutes by a slow and generally linear increase in lumen diameter with time that could be accounted for by an influx of fluid of the order of 0.5 micron3/micron2/min. It is possible that this phenomenon is due to a continued influx of fluid into the lumen until a junction opens between cells allowing a sudden loss of luminal fluid. The junction reseals and fluid begins to accumulate in the lumen. The phenomenon may be a three-dimensional analogue of the behavior of domes.
mesh_terms: D000818:Animals; D046508:Culture Techniques; D005110:Extracellular Space / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology* / Q000378:metabolism