title: A metalloproteinase associated with gelonin, a "ribosome inactivating protein".
abstract: Gelonin, a single-chain protein which inactivates eukaryotic ribosomes, becomes split into peptides when incubated with SDS. During the chromatographic purification of gelonin on carboxymethylcellulose three overlapping peaks emerge in the gelonin elution region, containing three proteins with small differences in apparent molecular weight (31,500, 30,000 and 29,200). All three proteins are endowed with inhibitory activity on protein synthesis and with proteinase activity, although with different specific activities, and all three give rise to the same peptides upon incubation with SDS, suggesting that they are isoforms of gelonin. The gelonin-associated proteinase acts only on gelonin, while it is inactive on the most common substrates for endoproteinases. The proteolytic activity is not inhibited by inhibitors of serine- or SH-proteinases, while it is completely abolished by chelating agents. Divalent cations restore the proteolytic activity inhibited by EDTA. The stability of the proteinase activity on exposure of gelonin to extreme values of pH or to prolonged incubation has been investigated. The inhibitory activity on protein synthesis and the proteinase activity are differently affected by these treatments.
mesh_terms: D000818:Animals; D002845:Chromatography; D004355:Drug Stability; D004492:Edetic Acid / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D006868:Hydrolysis; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D008666:Metalloendopeptidases; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism* / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D014176:Protein Biosynthesis; D011817:Rabbits; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013379:Substrate Specificity
chemical_list: D007461:Iodoacetates; D010446:Peptide Fragments; D010940:Plant Proteins; D011480:Protease Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; D012967:Sodium Dodecyl Sulfate; C025674:GEL protein, Gelonium multiflorum; D004492:Edetic Acid; D010450:Endopeptidases; D008666:Metalloendopeptidases; D005033:Ethylmaleimide; D019807:Iodoacetic Acid