Association of tobacco etch virus related RNA with chloroplasts in extracts of infected plants.
The RNA in various subcellular fractions of tobacco etch virus (TEV) infected tissue was analyzed for the presence of complementary viral RNA, and double-stranded viral RNA by hybridization with 32P-labeled viral RNA or cDNA probes. Although viral RNA was detected in several cellular fractions, the complementary RNA of full-length size was found exclusively associated with fractions containing chloroplasts. Treatment of RNAs with RNase before hybridization suggested that the virus-related complementary RNA was present in double-stranded form.
D002736:Chloroplasts / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000235:genetics*; D010947:Plants, Toxic; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D014026:Nicotiana / Q000382:microbiology*
D012330:RNA, Double-Stranded; D012367:RNA, Viral