title: The molecular weight-dependent distribution of urinary glycosaminoglycans in Werner's syndrome.
abstract: The macromolecular AGAG in the urine of patients with Werner's syndrome were analyzed by enzymatic methods after digestion with chondroitinases and Streptomyces hyaluronidase. The molecular weight-dependent distribution of the urinary AGAG has been determined by gel filtration on a Sephadex G-100 column. The distribution of HA and HS was predominant in the macromolecular fractions. Chondroitin sulfate isomers were prominent in the low molecular weight fractions but the ratio of the 4-type to the 6-type increased with decreasing molecular weight. These observations indicated that Werner's syndrome is a metabolic disorder of the molecular weight-dependent AGAG composition.
mesh_terms: D000328:Adult; D002809:Chondroitin Sulfates / Q000032:analysis; D002850:Chromatography, Gel; D003871:Dermatan Sulfate / Q000032:analysis; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006025:Glycosaminoglycans / Q000652:urine*; D006493:Heparin / Q000032:analysis; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D014898:Werner Syndrome / Q000652:urine*
chemical_list: D006025:Glycosaminoglycans; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D006493:Heparin; D002809:Chondroitin Sulfates