Transfer of chromosomal mutations to plasmids via Hfr-mediated conduction.
Nonconjugative plasmids can be transferred from an Hfr donor to a recipient by Hfr-mediated conduction. We found that this phenomenon can be employed to obtain a plasmid in which a mutation in a chromosomal gene has been transferred to the copy of that gene contained in the hybrid plasmid.
D001426:Bacterial Proteins / Q000235:genetics*; D002633:Chemotaxis; D002876:Chromosomes, Bacterial; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D009154:Mutation; D010957:Plasmids; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C041476:Trg protein, E coli