High-performance liquid chromatography as a tool for identification of linolenic acid hydroperoxide prepared with soybean lipoxygenase.
Linolenic acid hydroperoxide, enzymatically produced by soybean lipoxygenase from linolenic acid, was purified by high-performance liquid chromatography (HPLC) using a Supelco LC-8 (5 microns), 22 cm X 4.6 mm I.D. column with a solvent system of acetonitrile-tetrahydrofuran-0.1% phosphoric acid (50.4:21.6:28, v/v/v). The hydroperoxide was converted to several derivatives, the hydroxy, methyl, and stearate which were analyzed by HPLC and identified by gas chromatography-mass spectrometry. The 13-hydroperoxy linolenic acid was the major hydroperoxide produced by soybean lipoxygenase.
D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000032:analysis; D008042:Linolenic Acids / Q000032:analysis*; D008054:Lipid Peroxides / Q000032:analysis*; D008084:Lipoxygenase; D010545:Peroxides / Q000138:chemical synthesis; D013025:Glycine max / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet
D008042:Linolenic Acids; D008054:Lipid Peroxides; D010545:Peroxides; C025998:13-hydroperoxylinolenic acid; D006861:Hydrogen Peroxide; D008084:Lipoxygenase