title: Enzyme linked immunosorbent assay for lactoferrin. Plasma and tissue measurements.
abstract: Highly purified lactoferrin was obtained from human breast milk by sequential use of affinity chromatography and isoelectric focusing. IgG antibody to purified lactoferrin was used to develop a sensitive and reproducible enzyme linked immunosorbent assay. Characteristics of the assay included linearity over a wide range of lactoferrin concentration (3.125-200 micrograms/l) and sensitivity (lower range less than 1 microgram/l). The assay can be adapted for use on tissue cytosol as well as plasma. Healthy subjects showed plasma lactoferrin levels ranging from 187.5-450.1 micrograms/l. Pulmonary tuberculosis and acute pneumonia are associated with a 2-3-fold increase in plasma lactoferrin content while neutropenic subjects have markedly depressed lactoferrin concentrations. The assay will be useful for further delineation of lactoferrin and neutrophil function and turnover.
mesh_terms: D000906:Antibodies / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000097:blood; D001943:Breast Neoplasms / Q000032:analysis; D003600:Cytosol / Q000032:analysis; D004492:Edetic Acid / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000032:analysis* / Q000097:blood / Q000276:immunology; D007782:Lactoglobulins / Q000032:analysis*; D007970:Leukopenia / Q000097:blood; D008297:Male; D008895:Milk, Human / Q000032:analysis; D011018:Pneumonia, Pneumococcal / Q000097:blood; D014397:Tuberculosis, Pulmonary / Q000097:blood
chemical_list: D000906:Antibodies; D007782:Lactoglobulins; D004492:Edetic Acid; D007781:Lactoferrin