Correcting an immune-response deficiency by creating E alpha gene transgenic mice.
We have introduced a cloned Ek alpha gene into embryos of mice incapable of expressing their endogenous E alpha genes. The transcription of Ek alpha was accurate and tissue-specific in the resulting transgenic lines and, in macrophages, Ek alpha transcription could be induced by gamma-interferon. The injected gene conferred on the genetically deficient host strain the ability to mount an immune response to a synthetic polymer.
D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D011995:Recombination, Genetic; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma