[Detection of synthesized microsomal hemoproteins (cytochrome P-448) using autofluorography].
Simple and informative method for the elucidation of de novo synthesized forms of microsomal cytochrome P-448 induced by 3-methylcholanthrene and 2,3,7,8-tetrachlordibenzo-p-dioxine has been developed. The method is based on gel fluorography upon electrophoretic separation of microsomal proteins obtained from the liver of rats pre-treated with the inducers of monooxygenase system components and then with 14C-leucine. At least two forms of cytochrome P-448 (with molecular weight of 56000 and 53000) were shown to be de novo synthesized under the influence of 3-methylcholanthrene and 2,3,7,8-tetrachlodbibenzo-p-dioxine.
D000818:Animals; D019388:Cytochrome P-450 CYP1A2; D003580:Cytochromes / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D005453:Fluorescence; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D003580:Cytochromes; D000072317:Polychlorinated Dibenzodioxins; D008748:Methylcholanthrene; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2
Vyiavlenie sinteziruemykh mikrosomnykh gemoproteidov (tsitokhromov P-448) s pomoshch'iu avtofliuorografii.