Incorporation into the tissues and turnover of arachidonic acid after administration to normal and essential fatty acid deficient rats.
A comparison was made of the incorporation into the tissues and metabolism of [1-14C] arachidonic acid (AA) after i.v. administration to normal and EFA-deficient rats. At different times, ultra-thin whole body sections were prepared and the distribution of the radioactivity determined by autoradiograms. After 5 min, a considerable incorporation occurs in the following organs: subcutaneous and perispinal fat, liver, heart muscle, kidney and adrenal. The EFA deficient rats show a similar distribution but the radioactivity is longer retained. The total amount of radioactivity in the heart, liver, kidney and adrenal was measured at different times. A decline occurs in the heart, and an increase in the adrenal. In the urine, the highest amount of radioactivity is excreted on the first day. The excretion is lower in the EFA-deficient rats. Small amounts of radioactive metabolites with the chromatographic characteristics of PGE2 and 13,14 dihydro-15ketoPGE2 were isolated from urine. The amounts of 14CO2 produced were determined after the administration of [1-14C] AA. Half times were: 39 +/- 2.9 min in the EFA-deficient and 28 +/- 2.8 min in the normal rats. In the heart, AA is incorporated into phospholipids and neutral lipids. The following percentages were determined: phosphatidylinositol: 6.9 +/- 0.6%, phosphatidylcholine: 44 +/- 4.1%, phosphatidylethanolamine: 10.0 +/- 1.0%, neutral lipids: 9.3 +/- 1.6%. Several explanations can be given for the higher requirements of some tissues for AA. It could be, that this substance is used in the formation of particular membranes with a high AA content. Differences in the amounts of metabolites produced may also play a role.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000008:administration & dosage / Q000378:metabolism*; D001345:Autoradiography; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes; D005228:Fatty Acids, Essential / Q000172:deficiency*; D007275:Injections, Intravenous; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D005228:Fatty Acids, Essential; D002245:Carbon Dioxide; D016718:Arachidonic Acid