[Separation of isoenzymatic forms of human serum galactosyltransferase by chromatofocusing and isoelectric focusing. Application to cancerology].
Chromatofocusing is used to separate the multiple isoenzyme forms of human serum galactosyltransferase. At least 11 major peaks of activity are observed in normal sera, which are eluted between pH 4.3 and 6.9; a fraction of activity is eluted above pH 7.0. The normal patterns are compared with those obtained with sera from cancer patients and with an ascitic fluid. Chromatofocusing appears as resolutive as agarose isoelectric focusing.
D001201:Ascites / Q000201:enzymology; D002850:Chromatography, Gel; D005700:Galactosyltransferases / Q000097:blood*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D009369:Neoplasms / Q000201:enzymology; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase / Q000097:blood*
D007527:Isoenzymes; D005700:Galactosyltransferases; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase
Séparation des formes isoenzymatiques de la galactosyltransférase du sérum humain par chromatofocalisation et focalisation isoélectrique. Application à la cancérologie.