title: Insensitivity of erythrocyte 2,3-bisphosphoglycerate to changes in extracellular phosphate concentration in vitro.
abstract: Erythrocytes maintained in vitro under controlled conditions at pH 7.40 were unable to respond to changes in extracellular phosphate concentrations with changes in intracellular 2,3-bisphosphoglycerate content over an 8 h incubation period. Increased extracellular phosphate concentrations did not retard the rate at which 2,3-bisphosphoglycerate concentrations fell when erythrocytes were incubated at pH 7.10. Elevated extracellular phosphate concentrations did not increase the rate of recovery of intracellular 2,3-bisphosphoglycerate concentrations when erythrocytes were returned to pH 7.40 having been incubated at pH 7.10. The rate at which 2,3-bisphosphoglycerate concentrations increased under these latter conditions was too slow to counterbalance any effect that adjustment of blood pH may have on the position of the haemoglobin-oxygen dissociation curve during treatment of diabetic ketoacidosis.
mesh_terms: D019794:2,3-Diphosphoglycerate; D016883:Diabetic Ketoacidosis / Q000097:blood / Q000188:drug therapy; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D010710:Phosphates / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D004163:Diphosphoglyceric Acids; D010710:Phosphates; D019794:2,3-Diphosphoglycerate