[Production of human monoclonal antibodies against various antigens of erythrocyte stages of Plasmodium falciparum].
Peripheral blood lymphocytes from 20 individuals living in a malaria endemic area (Burkina Faso) were transformed with Epstein-Barr virus. No antigen specific selection, nor stimulation of B-cells were performed prior to transformation. 20 cell lines were established, 14 secreted polyclonal antibodies directed against erythrocytic stages of Plasmodium falciparum. 4 lines were cloned and the supernatant analysed and characterised against Plasmodium falciparum antigens.
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis*; D002460:Cell Line; D004912:Erythrocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008288:Malaria / Q000276:immunology*; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan
Production d'anticorps monoclonaux humains dirigés contre différents antigènes des stades érythrocytaires de Plasmodium falciparum.