Specific binding of tubulin to a guanine nucleotide-binding inhibitory regulatory protein in adenylate cyclase system, Ni.
A protein was isolated from the soluble fraction of rat brain by affinity chromatography with Sepharose to which guanine nucleotide-binding inhibitory regulatory protein in adenylate cyclase system, Ni, was immobilized. The molecular weight of this protein, specifically bound to the Ni-affinity column, was estimated as 54,000 on sodium dodecylsulfate-polyacrylamide gel electrophoresis. Alternately prepared tubulin also bound to the Ni-affinity column. The amino acid compositions of these proteins were also identical. It is strongly suggested that this Ni-binding cytosolic protein is tubulin.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D014404:Tubulin / Q000378:metabolism*
D000596:Amino Acids; D014404:Tubulin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases