[Study of peptide degradation in the serum by 1H-NMR].
Enzymatic degradation of hexapeptide Tyr-D-Ala-Gly-Phe-Leu-Arg in human serum has been investigated by 1H-NMR spectroscopy, and its pathways are suggested. Its final degradation products, tetra- and pentapeptide, are shown to be stable in human serum for several hours. The NMR spectroscopy application for pharmacokinetic studies is substantiated.
D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000097:blood; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009842:Oligopeptides / Q000097:blood*
D009842:Oligopeptides; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C040996:enkephalin-Leu, Ala(2)-Arg(6)-
Issledovanie degradatsii peptidov v syvorotke krovi metodom 1H-IaMR.