Carnitine transport in rat heart slices: I. The action of thiol reagents on the acetylcarnitine/carnitine exchange.
Rat heart slices show a permeability barrier that can be crossed by carnitine but not by sucrose and inulin. The integrity of thiol groups of heart cell membrane is essential for the uptake of carnitine. N-ethylmaleimide inhibits the transport into heart slices which is insensitive to Mersalyl. On the contrary both N-ethylmaleimide and Mersalyl inhibit acetyl carnitine/carnitine exchange. The amount of thiol groups titrated by the above reagents are related to the extent of exchange inhibition.
D000108:Acetylcarnitine / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002331:Carnitine / Q000031:analogs & derivatives* / Q000378:metabolism*; D002333:Carnitine Acyltransferases; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology*; D005033:Ethylmaleimide / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008634:Mersalyl / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D009579:Nitrobenzoates / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014166:Transferases / Q000378:metabolism
D009579:Nitrobenzoates; D008634:Mersalyl; D000108:Acetylcarnitine; D004228:Dithionitrobenzoic Acid; D014166:Transferases; D002333:Carnitine Acyltransferases; D005033:Ethylmaleimide; D002331:Carnitine