Minactivin expression in human monocyte and macrophage populations.
Adherent monolayer cultures of human blood monocytes, peritoneal macrophages, bone marrow macrophages, and colonic mucosa macrophages were examined for their ability to produce and secrete minactivin, a specific inactivator of urokinase-type plasminogen activator. All except colonic mucosa macrophages produced and secreted appreciable amounts of minactivin, but only blood monocytes were stimulated by muramyl dipeptide (adjuvant peptide) to increase production. The minactivin from each of these populations could be shown to preferentially inhibit urokinase-type plasminogen activator and not trypsin, plasmin, or "tissue"-type plasminogen activator (HPA66). A plasminogen-activating enzyme present in monocyte cultures appeared unaffected by the presence of minactivin and could be shown to be regulated independently by dexamethasone.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D001854:Bone Marrow Cells; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D003106:Colon; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010447:Peptide Hydrolases; D010537:Peritoneum / Q000166:cytology; D010960:Plasminogen Activators / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015849:Plasminogen Inactivators; D011092:Polyethylene Glycols; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D008070:Lipopolysaccharides; D015849:Plasminogen Inactivators; C039812:minactivin; D011092:Polyethylene Glycols; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D010447:Peptide Hydrolases; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator