Affinity labeling the ribosome with eukaryotic-specific antibiotics: (bromoacetyl)trichodermin.
Trichodermin, a eukaryotic-specific antibiotic, inhibits protein synthesis in Drosophila cells. We have synthesized a 14C-labeled bromoacetyl derivative of trichodermin that binds to Drosophila 80S ribosomes and once bound reacts covalently with ribosomal proteins. It does not react with rRNA. Three large-subunit proteins (L1, L3, and L24) and three small-subunit proteins (S3/S5, 2/3S, and S8) are labeled by [14C] (bromoacetyl)trichodermin. Reaction with each of these proteins can be competed by an excess of unmodified trichodermin, indicating that the labeling has occurred from the native binding site of the parent drug. One of the (bromoacetyl)trichodermin-labeled proteins (S8) is also labeled by photoactivated puromycin in the A site. A second protein (S3/S5) is found to be labeled by a P-site affinity reagent. The results suggest that the trichodermin binding site spans both the small and large subunits and portions of both the A and P sites. These data combined with previous studies on the A and P sites of Drosophila ribosomes have allowed us to construct a model of the protein locations in this important active site.
D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D004331:Drosophila melanogaster / Q000378:metabolism*; D007700:Kinetics; D010458:Peptidyl Transferases / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D012717:Sesquiterpenes / Q000378:metabolism*; D014243:Trichodermin / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology
D000345:Affinity Labels; D012717:Sesquiterpenes; D014243:Trichodermin; C047812:(bromoacetyl)trichodermin; D010458:Peptidyl Transferases