Enzymatic characterization of E.U.E. (embryonal human explants) cells adapted to hypertonic media.
E.U.E. cells (general population) were submitted to biochemical and cytoenzymatic tests to compare the enzymatic profile of E.U.E. cells (controls) with that of E.U.E. adapted to hypertonic medium. The adapted cells are characterized by very high oxoreductase activity (LDH, HBDH, G-6-P DH) and very high alkaline-phosphatase activity. Clones derived from general population were also submitted to biochemical tests to characterize those more strictly related to the enzymatic profile of adapted cells. The profile of clone N. 13 resembles on this respect that of the adapted cells. The high redox activity is a prerequisite supporting energy supply for osmotic work. The increased activity of plasma membrane enzymes of the adapted cells is also demonstrable in cells exposed for short time to salinity.
D000222:Adaptation, Physiological; D000469:Alkaline Phosphatase / Q000378:metabolism; D002460:Cell Line; D002999:Clone Cells / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D003470:Culture Media; D004622:Embryo, Mammalian; D004734:Energy Metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology / Q000502:physiology; D006651:Histocytochemistry; D006801:Humans; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D007770:L-Lactate Dehydrogenase / Q000378:metabolism
D003470:Culture Media; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase