Uptake and degradation of bovine testes beta-galactosidase by parenchymal and nonparenchymal rat liver cells.
The plasma half-life of beta-galactosidase in rat was about 1.5 min. Ten minutes after in vivo injection, 45% of the enzyme was recovered in liver, with hepatocytes and endothelial cells as the predominant cell types responsible for uptake. In vitro uptake of beta-galactosidase in hepatocytes and nonparenchymal liver cells was saturable, Ca2+-dependent and it could be partly inhibited by mannose or alpha-methyl-mannoside.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002458:Cell Fractionation; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004705:Endocytosis; D005696:Galactosidases / Q000378:metabolism*; D007275:Injections, Intravenous; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D037241:Mannose-Binding Lectins; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000378:metabolism / Q000502:physiology; D013737:Testis / Q000201:enzymology; D001616:beta-Galactosidase / Q000097:blood / Q000378:metabolism*
D037241:Mannose-Binding Lectins; D011971:Receptors, Immunologic; C035313:mannose-N-acetylglucosamine receptor; D005696:Galactosidases; D001616:beta-Galactosidase; D002118:Calcium