[Use of the Synpor membrane filter for the separation and determination of the number of somatic cells in the milk of dairy cows using the indole DNA filtration method].
The somatic cells of cow's milk were separated by filtering through nitrocellulose membrane filters (Synpor), pore size 2 to 5 micron. An indole reagent was added to the membrane filters with trapped cells, the mixture was warmed in boiling water bath for 20 min., then cooled, centrifuged (3500 X g, 15 min.), and optical density was measured at 490 nm. The relation 8 micrograms = 1 million cells was used for the conversion of DNA content in sample to the counts of cells. The average variation coefficient of the determination was 4.7% and the coefficient of correlation between the indole DNA filter method and direct microscopy on membrane filters was r = 0.997. Using the indole DNA filter method, the counts of somatic cells can be estimated from milk samples kept frozen or from filters with retained cells kept at 4 degrees C, or (for a short time--3 days) at 25 degrees C. The estimation is not distorted when the milk samples are stabilized with K2Cr2O7 or a mixture of K2Cr2O7 and HgCl2, if they are kept at 4 degrees C for eight days.
D000818:Animals; D002417:Cattle; D002452:Cell Count; D004247:DNA / Q000032:analysis; D003612:Dairying; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D007211:Indoles; D008892:Milk / Q000166:cytology*
D007211:Indoles; D004247:DNA
Vyuzití membránových filtrů Synpor pro separaci a stanovení poctu somatických bunĕk mléka dojnic indol DNA filtr metodou.