The effect of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (paf-acether) on the arterial wall.
The effect of a topical paf-acether superfusion over an injured arterial segment was assessed in the guinea-pig, using an opto-electronic in vivo thrombosis model allowing on-line quantification of small platelet thrombus dynamics. As compared to control, ADP-induced, thromboformation and behaviour, exogenous paf-acether causes a large, dense platelet thrombus, invaded and surrounded by numerous leukocytes, spreading widely over the adjoining, vacuolized, endothelium. Its embolization has to be forced with prostanoids, mepacrine, EDTA, or with a specific paf-acether antagonist (BN 52021). A few minutes after such forced embolization, a new thrombus starts growing at the same site, without renewal of the paf-acether superfusion. This phenomenon of spontaneous reappearance after forced embolization can be followed during several hours. Experiments with labelled paf-acether and the paf-acether antagonist indicate a possible endogenous paf-acether (or paf-acether-like) production triggered by superfusion with exogenous paf-acether.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D001158:Arteries / Q000187:drug effects*; D004224:Diterpenes; D004492:Edetic Acid / Q000494:pharmacology; D011464:Epoprostenol / Q000494:pharmacology; D046934:Ginkgolides; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007783:Lactones; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D011796:Quinacrine / Q000494:pharmacology; D013927:Thrombosis / Q000139:chemically induced*
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010936:Plant Extracts; D010972:Platelet Activating Factor; D000244:Adenosine Diphosphate; D004492:Edetic Acid; D011464:Epoprostenol; C045856:ginkgolide B; D000527:Alprostadil; D011796:Quinacrine