Production of cloned human leukocyte interferon by Bacillus subtilis: optimal production is connected with restrained growth.
Bacillus subtilis, transformed with a plasmid containing the human alpha-2 (leukocyte) interferon gene, was cultivated in batch and continuous culture in a complex medium. In continuous culture with dissolved oxygen of less than 10% of air saturation, the extracellular interferon titer decreased sharply when the growth rate was lower or higher than the optimal one (mu = 0.14 h-1). Thus, a relatively low growth rate was best for extracellular interferon production, and oxygen limitation enhanced interferon production. The mean output rate in batch culture after successful harvest was 20 X 10(6) IU/liter per h and the maximal output rate in continuous culture was 14 X 10(6) IU/liter per h.
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003001:Cloning, Molecular; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D010100:Oxygen / Q000494:pharmacology; D010957:Plasmids; D014170:Transformation, Genetic
D007370:Interferon Type I; D010100:Oxygen