Mitomycin-C induced improvement in superficial bladder tumors: a morphologic and immunohistochemical evaluation.
Twenty bladder biopsies from ten patients with superficial transitional cell carcinoma were obtained prior to and following therapy with intravesical mitomycin-C. Each biopsy was evaluated histologically and immunohistochemically for A, B, H blood group antigen (BGA) expression. A, B and H blood group antigens were identified using monoclonal anti-A, B and H antibody and were localized with the avidin-biotin-peroxidase complex (ABC) technique. Particular attention was directed toward flat urothelial lesions and the urothelium adjacent to papillary tumors. Routine histologic evaluation showed improvement in post-therapy biopsies compared to corresponding pre-therapy biopsies in 7/10 cases but was equivocal in 3/10 cases. Immunohistochemical evaluation, however, showed improvement in all 10 cases, as judged by increased urothelial BGA expression. This increase in urothelial BGA expression after intravesical mitomycin-C therapy suggests a therapy induced improvement in dysplastic urothelium which was not uniformly evident on routine histologic examination.
D001706:Biopsy; D002295:Carcinoma, Transitional Cell / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007519:Isoantigens / Q000032:analysis; D016685:Mitomycin; D008937:Mitomycins / Q000627:therapeutic use*; D001743:Urinary Bladder / Q000276:immunology / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology
D007519:Isoantigens; D008937:Mitomycins; D016685:Mitomycin