title: Ultracytochemical nuclear calcium distribution in pancreatic B cells: its relation to glucose-stimulated insulin secretion.
abstract: Calcium distribution in pancreatic B cells was studied, with the aid of the pyroantimonate technique, at different times of glucose-induced secretory activity in the perfused rat pancreas. Specificity of the pyroantimonate precipitates for calcium was assessed by EGTA cross-incubation. Quantitative studies for these calcium pyroantimonate precipitates were performed by a morphometric technique. Pyroantimonate precipitates within the B cell show both time and glucose dependence. At any time-point studied, in the nucleus as well as in other organelles, they were more numerous when glucose was increased in the medium from 3.3 to 16.6 mmol/l. The total number of nuclear calcium pyroantimonate precipitates rose sharply at the time corresponding to the refractory period, falling after that to almost the number found at the prestimulatory period. Otherwise, glucose significantly modifies the temporal distribution of precipitates bound to euchromatin, heterochromatin and perichromatin. These changes in nuclear calcium pyroantimonate precipitates during different periods of B cell secretory activity may indicate an active role of the cation in some nuclear regulatory function during glucose-induced release of insulin.
mesh_terms: D000818:Animals; D000965:Antimony / Q000032:analysis; D002118:Calcium / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D006651:Histocytochemistry; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010179:Pancreas / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D014018:Tissue Distribution
chemical_list: D007328:Insulin; C009133:pyroantimonate; D004533:Egtazic Acid; D000965:Antimony; D005947:Glucose; D002118:Calcium