[Effective method of transduction with virulent phage pf16 using specific mutants of Pseudomonas putida PpG1].
A procedure of simple selection of Pseudomonas putida PpG1 mutants is described. The mutants can be used for transduction with virulent pf16 phage, giving reliable results. The frequency of transduction of chromosomal markers ilv and trp was 10(-6). Also, transduction of plasmid RP4 with phage pf16 was shown with the frequency of 10(-7).
D001435:Bacteriophages / Q000235:genetics* / Q000472:pathogenicity; D002874:Chromosome Mapping; D005821:Genetic Techniques; D066298:In Vitro Techniques; D009154:Mutation; D011549:Pseudomonas / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D014161:Transduction, Genetic; D014774:Virulence
Effektivnyĭ metod transduktsii virulentnym fagom pf16 s ispol'zovaniem spetsificheskikh mutantov Pseudomonas putida PpG1.