[Determination of human serum galactosyltransferase using a kinetic spectrophotometric technic].
A kinetic spectrophotometric method in which galactose transfer is coupled to the production of NADH, has been adapted to the assay of galactosyltransferase activity in human serum. Under the described conditions, the rate of NADH production is linear with regard to enzyme concentration, and directly depends upon the various biochemical factors which control galactosyltransferase activity.
D000117:Acetylglucosamine / Q000378:metabolism; D005700:Galactosyltransferases / Q000037:antagonists & inhibitors / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D009243:NAD / Q000096:biosynthesis; D013053:Spectrophotometry; D014531:Uridine Diphosphate Galactose / Q000378:metabolism
D009243:NAD; D014531:Uridine Diphosphate Galactose; D008345:Manganese; D005700:Galactosyltransferases; D000117:Acetylglucosamine
Dosage de la galactosyltransférase du sérum humain à l'aide d'une technique cinétique spectrophotométrique.