[Isolation and properties of human leukocyte interferon obtained by microbial synthesis].
Highly sensitive, effective and simple methods for determination of leukocytic interferons were developed. The methods are based on formation of immune complexes of interferon with anti-interferon monoclonal and polyclonal antibodies immobilized on an insoluble basis. As a result of interferon alpha 2 purification from the biomass of the interferon-producing bacteria the preparative amounts of the protein were obtained. These amounts were sufficient for the interferon structural and physico-chemical analysis. The isolated interferons are characterized by high stability on storage and may serve a basis for preparation of pharmaceutical agents.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007370:Interferon Type I / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D011817:Rabbits; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D013696:Temperature
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D011994:Recombinant Proteins
Vydelenie i svoĭstva chelovecheskogo leĭkotsitarnogo interferona, poluchennogo mikrobnym sintezom.