Complementation analysis of beta-galactosidase deficiency by means of histochemical method.
Cultured skin fibroblasts from 4 beta-galactosidase-deficient patients with different clinical features were hybridized and beta-galactosidase activities were measured using 4-methylumbelliferyl (4MU)-derivate and by means of indigogenic method. Compared with the assays of beta-galactosidase activity using 4MU-derivate, more clear-cut evidence was obtained in the indigogenic method for a judgement of complementation.
D002478:Cells, Cultured; D005347:Fibroblasts / Q000201:enzymology; D005696:Galactosidases / Q000378:metabolism*; D005816:Genetic Complementation Test; D006651:Histocytochemistry; D006801:Humans; D007787:Lactose Intolerance / Q000201:enzymology* / Q000235:genetics; D012867:Skin / Q000201:enzymology*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D005696:Galactosidases; D001616:beta-Galactosidase