title: Synthesis of viral DNA forms in Nicotiana plumbaginifolia protoplasts inoculated with cassava latent virus (CLV); evidence for the independent replication of one component of the CLV genome.
abstract: Totipotent leaf mesophyll protoplasts of Nicotiana plumbaginifolia, Viviani were inoculated with cassava latent virus (CLV) or with full length copies of CLV genomic DNAs 1 and 2 excised from replicative forms of M13 clones. Virus specific DNAs began to appear 48-72h after inoculation with virus or cloned DNAs, coincident with the onset of host cell division. Infected cells accumulated supercoiled forms of DNAs 1 and 2 as well as progeny single-stranded (ss) virion (+) sense DNAs representing each component of the genome. Both supercoiled and ss molecules were synthesised by cells inoculated with cloned DNA 1 alone but DNA 2 failed to replicate independently.
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D004261:DNA Replication; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D004278:DNA, Superhelical / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis*; D010942:Plant Viruses / Q000235:genetics*; D010947:Plants, Toxic; D014026:Nicotiana; D014779:Virus Replication
chemical_list: D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D004279:DNA, Viral