Liquid chromatographic determination of mexiletine and tocainide in human plasma with fluorescence detection after reaction with a modified o-phthalaldehyde reagent.
A liquid chromatographic procedure for the determination of mexiletine or tocainide in human plasma is described. Plasma, after the addition of a homologue of mexiletine or of tocainide, is extracted with dichloromethane. The extract is evaporated and reconstituted in a non-aqueous o-phthalaldehyde--mercaptoethanol reagent. An aliquot of the solution is chromatographed on a reversed-phase Ultrasphere-octyl column. The peaks are detected by fluorescence (lambda ex = 350 nm and lambda em = 445 nm). The fluorescent derivatives of the drugs and internal standards are stable at room temperature and give symmetrical single peaks. Use of fluorescamine as a reagent to prepare fluorescent derivatives of mexiletine and tocainide prior to chromatography is also evaluated.
D002853:Chromatography, Liquid; D004355:Drug Stability; D006801:Humans; D008012:Lidocaine / Q000031:analogs & derivatives* / Q000097:blood; D008801:Mexiletine / Q000097:blood*; D011437:Propylamines / Q000097:blood*; D013050:Spectrometry, Fluorescence; D016677:Tocainide; D009764:o-Phthalaldehyde
D011437:Propylamines; D008801:Mexiletine; D016677:Tocainide; D009764:o-Phthalaldehyde; D008012:Lidocaine