Preparation and specificity of avian anti-GM2(NeuGc) ganglioside antiserum.
Antibodies to N-glycolyl neuraminic acid-containing GM2 ganglioside, GM2(NeuGc), were prepared by immunizing chickens. The specificity of the antibodies was examined by the double immunodiffusion test and solid-phase radioimmunoassay (RIA). One(C-4) of two antisera produced did not cross-react with GM3(NeuGc) but the other(C-3) did as assessed by the double immunodiffusion test. In RIA, the antibody activity of C-4 antiserum was detected only in the IgG fraction. Specificity of the serum was examined using authentic glycolipids which were structurally related to GM2(NeuGc). The antiserum showed a high specificity for the homologous ganglioside by either an RIA or an inhibition assay. This antiserum is a useful tool for the detection of GM2(NeuGc) in human and animal tissues under normal and/or disease condition.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D002645:Chickens; D003429:Cross Reactions; D005678:G(M2) Ganglioside / Q000031:analogs & derivatives / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D005779:Immunodiffusion; D011863:Radioimmunoassay
D000906:Antibodies; D005732:Gangliosides; D005678:G(M2) Ganglioside; C045702:GM2 N-glycolylneuraminic acid ganglioside