Interactions of Plasmodium berghei-infected erythrocytes with complement.
Incubation of radioactively labeled parasitized (Plasmodium berghei) erythrocytes (PE) with adherent peritoneal exudate cells in the presence of 10% (v/v) fresh mouse serum (NMS) resulted in the uptake of a proportion of radioactive material (PE). Inactivation of the added serum by heat or zymosan treatment resulted in diminished uptake of radioactivity. These results suggest that PE activated complement. Incubation of fresh NMS with PE reduced the hemolytic complement level of the serum as shown by its subsequent decreased ability to lyse antibody-coated rabbit red blood cells. No such effect was found when uninfected erythrocytes from either infected or uninfected blood were preincubated with fresh NMS. Thus, PE or PE-derived material activated complement. Addition of EGTA during incubation of fresh NMS with PE did not inhibit the decrease in complement level. This indicated that complement was activated by the alternative pathway. Complement levels decreased even when fresh NMS and PE were incubated in the presence of EDTA (which inhibits both classical and alternative pathway activation), suggesting that a complement activating factor (or a complement inhibitor) was released from the PE. However, lysis of PE after incubation with either fresh rabbit or guinea pig serum did not occur unless anti-mouse erythrocyte antibody was added. The production of a complement-activating factor by PE might explain part of the decreasing complement levels during infection and might enable the parasite to escape from a complement-mediated defense mechanism of the host.
D000818:Animals; D001202:Ascitic Fluid; D003167:Complement Activation; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology*; D006168:Guinea Pigs; D006358:Hot Temperature; D008288:Malaria / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D010587:Phagocytosis; D010962:Plasmodium berghei / Q000276:immunology*; D011817:Rabbits; D015054:Zymosan / Q000494:pharmacology
D004533:Egtazic Acid; D015054:Zymosan; D004492:Edetic Acid