[Biochemical characteristics of adhesion factors isolated from the retina of 8-day-old chick embryos].
While isoelectrofocusing the extracts obtained after dissociation of the retina of eight day old chick embryos, two active protein fractions were found: AF I with pI 3 and AF II with pI below 3. The content of these fractions in the extracts depended on the method of dissociation: treatment with EDTA increased the content of AF II, whereas trypsin treatment increased the yield of AF I. After electrophoresis in the denaturating conditions both fractions were divided in two components. The effect of AF I on aggregation of retinal cells, unlike that of AF II, does not depend on the presence of bivalent cations in the medium and is realized within 1 h of interaction with the cells at 4 degrees. It is suggested that the retinal extracts of eight day old chick embryos contain substances providing two types of adhesiveness: Ca2+-independent (AF I) and Ca2+-dependent (AF II).
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002458:Cell Fractionation; D002642:Chick Embryo / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D007525:Isoelectric Focusing; D011485:Protein Binding / Q000187:drug effects; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
D005136:Eye Proteins; D004492:Edetic Acid; D014357:Trypsin
Biokhimicheskaia kharakteristika faktorov adgezii, vydelennykh iz setchatki 8-sutochnykh kurinykh zarodysheĭ.