Suppression of the in vitro humoral immune response by chrysotile asbestos.
The direct addition of UICC chrysotile B asbestos to spleen cell cultures produced a dose-dependent suppression of the antibody-forming cell (AFC) response to the T-dependent antigen, sheep erythrocytes. Concentrations of 0.1, 1, 10, and 100 micrograms/ml, which had no effect on either cell number or viability, suppressed the in vitro response by 12, 43, 71, and 95%, respectively. By separating spleen cells into nonadherent and plastic-adherent populations, the effects of asbestos on each cell type were studied utilizing reconstituted cultures. Treatment of adherent cells for 1 hr with asbestos and reconstitution with untreated nonadherent cells resulted in a dose-dependent suppression of the AFC response similar to that observed when asbestos was added to whole spleen cultures. Reconstitution studies were also conducted with spleen cell populations from animals administered asbestos in vivo. In cultures reconstituted with adherent cells from asbestos-treated mice and nonadherent cells from saline-treated animals, the AFC response was significantly suppressed. The T-dependent AFC response with reconstitution of adherent cells from saline-treated mice and nonadherent cells from asbestos-exposed animals was no different from reconstituted saline controls. Both adherent alveolar and pleural cells were capable of reconstituting the T-dependent AFC response with nonadherent spleen cells and demonstrated reduced immunological capability when exposed to asbestos fibers. The results of this investigation have identified the adherent spleen cell (macrophage) as the target cell type responsible for asbestos-induced immunosuppression of the in vitro T-dependent antibody-forming cell response.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001194:Asbestos / Q000276:immunology / Q000633:toxicity*; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D007075:Immunoglobulin M / Q000276:immunology; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D008297:Male; D008623:Mercaptoethanol / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000187:drug effects* / Q000276:immunology
D007075:Immunoglobulin M; D001194:Asbestos; D008623:Mercaptoethanol