title: Cholecystokinin is not a peripheral satiety signal in the dog.
abstract: The doses of cholecystokinin (CCK) that have been shown to cause satiety after peripheral administration are pharmacological and whether "physiological" doses of exogenous CCK or endogenously released CCK have a satiety effect is not known. The purpose of the present study is threefold: to compare the potency of endogenous and exogenous CCK for their satiety, cholecystokinetic, and pancreozyminic actions; to determine whether small doses of CCK in the presence of gastric distension cause satiety; and to test the satiety effect of CCK administered centrally into the lateral cerebral ventricle. Dogs were prepared with cerebroventricular guides and esophageal and duodenal fistulas (n = 4), with chronic bile fistulas (n = 8), and chronic pancreatic fistulas (n = 7). Satiety effect was quantified in the esophageal fistula dog by the amount of blenderized food sham fed within 7.5 min. Duodenal perfusion of fat, used as a releaser of endogenous CCK, stimulated pancreatic protein, and gallbladder contraction with D50 values of 1.5 and 0.3 mmol/h, respectively, but had no effect on the volume sham fed (316 +/- 82 ml/min without and 371 +/- 41 ml/min with the maximal dose of sodium oleate infused). The D50 values of CCK-8 (pmol X kg-1 X h-1) for satiety and for stimulation of pancreatic enzyme secretion and gallbladder contraction were 440, 120, and 22, respectively. Injection of CCK-8 into the lateral cerebral ventricle inhibited sham feeding in a dose-dependent manner. We conclude that duodenal fat in doses producing maximal gallbladder and pancreatic stimulation has no satiety effect in the dog.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004435:Eating; D005704:Gallbladder / Q000187:drug effects / Q000378:metabolism; D000882:Haplorhini; D006801:Humans; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012527:Satiation / Q000187:drug effects* / Q000502:physiology; D012756:Sheep; D012844:Sincalide / Q000494:pharmacology*; D013270:Stomach / Q000502:physiology
chemical_list: D009829:Oleic Acids; D019301:Oleic Acid; C013173:osteum; D012844:Sincalide; D001663:Bilirubin