Glycosidase activities in the rat liver microsomes.
The glycosidase activities in a rat liver microsome fraction were investigated in comparison with those in a submitochondrial fraction. The activities of beta-N-acetylglucosaminidase, beta-galactosidase and beta-glucosidase in the former were distinguished from those in the latter on the basis of solubilization pattern, pH optimum, thermal stability and elution pattern of concanavalin A-Sepharose chromatography. It is suggested that these activities are not due to the contamination of lysosomal enzymes but intrinsic to microsomes.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008361:Mannosidases / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013367:Submitochondrial Particles / Q000201:enzymology; D043323:alpha-Mannosidase; D001616:beta-Galactosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D006026:Glycoside Hydrolases; D008361:Mannosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D000118:Acetylglucosaminidase