title: Binding of Al by protein in plasma of patients on maintenance hemodialysis.
abstract: Gel filtration of plasma from hemodialysis patients, with use of reagents and apparatus with carefully minimized background Al concentrations, reproducibly showed a single peak for Al, corresponding exactly to the elution position of transferrin. The Al/transferrin molar ratio in adjacent fractions was constant (mean 0.126, SE 0.006) in replicate experiments. In contrast, the association of Al with albumin varied. Using both equilibrium dialysis and gel-filtration techniques, in the presence and absence of calcium or phosphate, we could demonstrate no significant binding of Al by human albumin at Al concentrations of 1 to 12 mumol/L. We saw no Al peak in pooled, concentrated, low-molecular-mass fractions of plasma gel-filtered on Sephadex G-50. Evidently, transferrin is the sole Al-binding protein in plasma of hemodialysis patients.
mesh_terms: D000535:Aluminum / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D002118:Calcium; D002850:Chromatography, Gel; D004492:Edetic Acid; D006801:Humans; D010710:Phosphates; D006435:Renal Dialysis; D012709:Serum Albumin / Q000032:analysis; D014168:Transferrin / Q000097:blood
chemical_list: D001798:Blood Proteins; D010710:Phosphates; D012709:Serum Albumin; D014168:Transferrin; D004492:Edetic Acid; D000535:Aluminum; D002118:Calcium