Stability of ascorbate in urine: relevance to analyses for ascorbate and oxalate.
Ascorbate is unstable in urine at room temperature at pH values ranging from 1 to 12. At pH 7 and above, oxalate is generated in amounts directly proportional to the ascorbate concentration. In 12 different urines, adjusted to pH 12 and incubated for 20 h at room temperature, there was a significant correlation between the amount of oxalate formed and the initial ascorbate concentration (r = 0.97, p less than 0.01). The mean (+/- SD) concentration of oxalate (1.32 +/- 0.70 mmol/L) formed during this period approximated the initial ascorbate concentration (1.57 +/- 1.09 mmol/L). Disodium EDTA, 10 mmol/L final concentration, stabilizes ascorbate in urine and inhibits its conversion to oxalate at pH values of 4.4 to 7.0 during a 24-h period. We therefore propose that urine specimens for ascorbate and oxalate analyses be collected with disodium EDTA present such as to give about this final concentration.
D001205:Ascorbic Acid / Q000652:urine*; D004492:Edetic Acid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010070:Oxalates / Q000652:urine*; D019815:Oxalic Acid; D013696:Temperature; D013997:Time Factors
D010070:Oxalates; D019815:Oxalic Acid; D004492:Edetic Acid; D001205:Ascorbic Acid