Cloning of opal suppressor tRNA genes of a filamentous fungus reveals two tRNASerUGA genes with unexpected structural differences.
The informational suppressors su4-1 and su8-1 of Podospora anserina were isolated by transformation of Schizosaccharomyces pombe UGA mutants. The DNA sequence revealed that they were opal (UGA) suppressor tRNAs. Wild-type alleles were also isolated by hybridization. The DNA sequence showed that they both encode species of tRNASerUGA. The gene SU8 has an 18-bp intervening sequence and its primary sequence is very different from that of SU4.
D001483:Base Sequence; D003001:Cloning, Molecular; D005658:Fungi / Q000235:genetics; D005800:Genes, Fungal; D012343:RNA, Transfer / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012694:Serine; D013489:Suppression, Genetic
D012694:Serine; D012343:RNA, Transfer