title: An evaluation of 11 red cell elution procedures.
abstract: Eleven elution procedures were evaluated for their efficacy in recovering antibody from red cells sensitized with immunoglobulin. The procedures evaluated included eight published methods (Lui, heat, digitonin-acid, ether, chloroform, dichloromethane, xylene, and alcohol freeze-thaw) and three commercial elution kits (EluAid, Ortho Diagnostic Systems, Inc.; Elution Solution, Biological Corporation of America; and Elu-Kit II, Gamma Biologicals, Inc.). The xylene elution method proved to be the most effective method, followed by Elu-Kit II, chloroform, dichloromethane, and digitonin-acid. The other six methods evaluated were not optimal based on the suitability of each procedure and the calculated sensitivity.
mesh_terms: D001789:Blood Group Antigens; D001803:Blood Transfusion / Q000379:methods; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000302:isolation & purification*
chemical_list: D001789:Blood Group Antigens; D007518:Isoantibodies