Characterization of hepatocytes from carcinogen-treated rats by two-parametric flow cytometry.
Hepatocytes in the adult rat are normally mostly tetraploid, but sequential carcinogen treatment (diethylnitrosamine/2-acetylaminofluorene) induces the development of a predominantly diploid hepatocyte population. By two-parametric flow cytometry (simultaneous measurement of DNA and protein content within the same cell) it could be shown that diploid hepatocytes from carcinogen-treated rats had only half the size (protein content) of the tetraploid cells from the same liver, but the same size as diploid hepatocytes from normal rats.
D015073:2-Acetylaminofluorene / Q000633:toxicity*; D000818:Animals; D004247:DNA / Q000032:analysis; D004052:Diethylnitrosamine / Q000633:toxicity*; D005434:Flow Cytometry / Q000379:methods; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D004052:Diethylnitrosamine; D004247:DNA; D015073:2-Acetylaminofluorene