title: Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes.
abstract: Hepatocytes of Salmo salar in primary culture form confluent monolayers and can be maintained at 11 degrees centigrade in serum-free medium for 8 days with minimal cell loss. Cultured hepatocytes from immature male salmon contain estrogen receptor both in nuclear and cytosol fractions (2000 and 2400 cites/cell, respectively). A single addition of estradiol results in an increase in the nuclear receptor to a level of 23 000 sites/cell after 24 h. This nuclear receptor concentration is similar to that in liver of estrogen-treated salmon in vivo, and is much higher than has been found for any other egg-laying vertebrate. The cultured salmon hepatocytes thus represent a highly sensitive system for the study of estrogen receptor dynamics and vitellogenesis in vitro.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D012474:Salmon; D014818:Vitellogenesis
chemical_list: D011960:Receptors, Estrogen; D004958:Estradiol