title: Differential modification of oxic and anoxic components of radiation damage in Bacillus megaterium spores by caffeine.
abstract: Studies were carried out on the effect of caffeine on the X-irradiation sensitivity of B. megaterium spores with the following results: Caffeine exerts a concentration-dependent modifying action on oxygen-dependent components of X-ray-induced damage in B. megaterium spore suspensions causing an 'over-O2 effect' at about 1 X 10(-4) mol dm-3, and as the concentration is increased to 1 X 10(-3) mol dm-3 or above, a small but consistent protection is seen. In the absence of O2, at a wide range of concentrations (8.5 X 10(-5) to 1 X 10(-1) mol dm-3), caffeine enhances the inactivation constant, k, from 1.17 to about 1.50 kGy-1. Both ethanol and t-butanol (5 X 10(-2) mol dm-3) remove the 'over O2-effect' produced by 1.10(-4) mol dm-3 caffeine in O2; such an effect, however, is not accompanied by reduction in the H2O2 concentrations in the spore suspensions. Ethanol prevents caffeine-induced anoxic sensitization, as well as H2O2 buildup. t-BuOH has no influence on either the low dose part of the log fraction survival curve or on the H2O2 yield in the spore suspensions. Caffeine reacts with radiation-induced eaq and .OH with rate constants of 1.5 X 10(10) and 6.9 X 10(9) dm3 mol-1s-1, respectively.
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001410:Bacillus megaterium / Q000187:drug effects / Q000528:radiation effects*; D002110:Caffeine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D011836:Radiation Tolerance; D013171:Spores, Bacterial / Q000187:drug effects / Q000528:radiation effects
chemical_list: D002110:Caffeine