Homogeneous, liposome-based assay for total complement activity in serum.
This is a rapid, homogeneous, liposome-based assay for total complement activity in human serum. Liposome-encapsulated enzyme is unmasked by the action of complement on liposomes carrying surface-bound immune complexes. The amount of unmasked enzyme, proportional to the concentration of added complement, is quantified by measuring the absorbance of enzymically produced product at 410 nm. Complement activity in serum samples is extrapolated from a standard curve generated from dilutions of a guinea pig serum containing a known activity of complement. Interassay CVs were less than 7.0% and intra-assay CVs less than 2.8% for serum pools with complement activities spanning the normal range. Test results correlate as well with those of the hemolytic complement test (r = 0.80) as the latter correlates with itself (r = 0.82), and also correlate reasonably with measurements of complement components C3 (r = 0.62) and C4 (r = 0.74). Values for a normal population are reported. Advantages of this test include stability of reagents, speed, accuracy, simplicity, and avoidance of radioisotopes.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000276:immunology; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis*; D006801:Humans; D008081:Liposomes / Q000276:immunology*; D009596:Nitrophenols / Q000032:analysis / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D012016:Reference Values; D013053:Spectrophotometry
D000936:Antigen-Antibody Complex; D003176:Complement C3; D003181:Complement C4; D008081:Liposomes; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D003165:Complement System Proteins; D000469:Alkaline Phosphatase; C024836:4-nitrophenol