title: Thyroid hormone deiodinases in purified primary glial cell cultures.
abstract: Iodothyronine metabolism was studied in neuroglial cells prepared from neonatal rat cerebri. Astrocytes account for nearly all of the deiodinase activity in mixed glial cell cultures. The predominant pathway is 5-deiodination, which closely resembles the enzyme activity in homogenates of cerebral cortex. Astrocytes cultured in serum-free chemically defined medium show a gradual decrease in 5-deiodination and an increase in 5'-deiodination. Exposure of cells to triiodothyronine partially reverses these changes. Thus, astrocytes contain enzymes for both major deiodinative pathways and may play a role in the regulation of thyroid hormone levels in the brain.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000201:enzymology; D002478:Cells, Cultured; D007453:Iodide Peroxidase / Q000378:metabolism*; D009457:Neuroglia / Q000201:enzymology*; D009836:Oligodendroglia / Q000201:enzymology; D051381:Rats; D013687:Telencephalon; D013974:Thyroxine / Q000378:metabolism*; D014284:Triiodothyronine / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D014284:Triiodothyronine; D007453:Iodide Peroxidase; D013974:Thyroxine