DNA metabolism during infection of Anacystis nidulans by cyanophage AS-1. VI. Effect of hydroxyurea and nalidixic acid on the development of cyanophage AS-1.
The use of the DNA inhibitors hydroxyurea (HU) and nalidixic acid (NAL) to elucidate patterns of DNA metabolism in AS-1 infected A. nidulans have led to several conclusions. First, HU and NAL at concentrations of 500 and 100 micrograms/ml, respectively, inhibited DNA synthesis in synchronized A. nidulans. Protein and chlorophyll synthesis remained essentially unchanged as did turbidity increases. Second, the complete burst size of the cyanophage AS-1 was severely affected by HU and NAL treatment and burst sizes of 8 and 2.6, respectively, were obtained. Third, inhibitor treatment of infected cultures caused a premature release of phage at 12 h instead of 16 h post infection. Finally, HU treatment of the host cell had no effect on the formation of PIL-DNA, whereas NAL pretreatment blocked its formation.
D002734:Chlorophyll / Q000096:biosynthesis; D000458:Cyanobacteria / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D004279:DNA, Viral / Q000096:biosynthesis; D006918:Hydroxyurea / Q000494:pharmacology*; D009268:Nalidixic Acid / Q000494:pharmacology*; D014176:Protein Biosynthesis; D014780:Viruses / Q000187:drug effects / Q000254:growth & development*
D004279:DNA, Viral; D002734:Chlorophyll; D009268:Nalidixic Acid; D004247:DNA; D006918:Hydroxyurea