Effects of retinol on chromatin structure.
The effect of retinol is studied in 3T3 cultured cells. The vitamin induces a decreased rate of cell proliferation and an augmented sensitivity of chromatin to DNase I digestion. Biochemical analyses of chromosomal components establish that the rates of radioactive acetate uptake and turnover on histones are increased leaving unaltered the steady-state level of histone acetylation. The presence of retinol in the culture medium also causes the disappearance of a protein of Mr 20 000, which is co-extracted with the high-mobility-group proteins. The observed changes in chromatin structure and composition are reversible when retinol is removed from the culture medium.
D000107:Acetylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002843:Chromatin / Q000187:drug effects* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D003720:Densitometry; D003850:Deoxyribonuclease I; D006657:Histones / Q000378:metabolism; D006868:Hydrolysis; D007120:Immunochemistry; D008596:Menotropins / Q000302:isolation & purification; D051379:Mice; D014801:Vitamin A / Q000494:pharmacology*
D000596:Amino Acids; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D014801:Vitamin A; D008596:Menotropins; D003850:Deoxyribonuclease I