title: Influence of environmental factors on the expression of phenotypic characters by chicken dorsal root ganglion cells in culture.
abstract: Primary sensory neurons were grown under four conditions of culture. The influence of nonneuronal cells, horse serum or both was studied on the phenotypic expression of certain neuronal subpopulations. The number of neurons expressing acetylcholinesterase, alpha-bungarotoxin-binding sites or a high uptake capacity for glutamine was enhanced by nonneuronal cells. The horse serum increases the neuronal subpopulation exhibiting a carbonic anhydrase activity. Certain phenotypic changes fit conditions consistent with an epigenetic induction rather than a cell selection.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001790:Blood Physiological Phenomena; D002038:Bungarotoxins / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology*; D005973:Glutamine / Q000378:metabolism; D006736:Horses; D009475:Neurons, Afferent / Q000145:classification* / Q000166:cytology / Q000201:enzymology; D010641:Phenotype; D011950:Receptors, Cholinergic / Q000032:analysis; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: D002038:Bungarotoxins; D003470:Culture Media; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; D005973:Glutamine; D000110:Acetylcholinesterase; D002256:Carbonic Anhydrases