title: Kidney independent serine synthesis in the rat. Effect of dietary serine and glycine restriction.
abstract: The potential role of the kidney in renal serine synthesis was elucidated in rats following dietary serine as well as serine and glycine depletion for 6 weeks. Serine deficient diet caused a significant rise in blood serine and glycine values. In contrast, blood glycine concentration decreased significantly following serine and glycine depletion, whereas blood serine levels remained unchanged. Bilateral nephrectomy caused a significant decrease of blood serine values of control and serine deficient rats. Serine concentration, however, was not affected by bilateral nephrectomy following serine and glycine deficiency indicating kidney-independent serine synthesis under these conditions. There was a significant increase of blood lactate and pyruvate levels under serine and serine/glycine deficient diet compared with control animals, whereas blood ketone bodies fell. In contrast, 24 h after bilateral nephrectomy blood ketone bodies rose and blood lactate and pyruvate concentrations decreased significantly. 24 h after bilateral nephrectomy there was a marked rise in blood citrate values of female rats compared with male animals indicating sex dependency of kidney citrate metabolism in the rat.
mesh_terms: D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000097:blood; D000818:Animals; D002951:Citrates / Q000097:blood; D019343:Citric Acid; D005260:Female; D005998:Glycine / Q000097:blood / Q000172:deficiency*; D006885:Hydroxybutyrates / Q000097:blood; D007656:Ketoglutaric Acids / Q000097:blood; D007668:Kidney / Q000378:metabolism*; D007773:Lactates / Q000097:blood; D008297:Male; D009392:Nephrectomy; D011773:Pyruvates / Q000097:blood; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D012694:Serine / Q000096:biosynthesis* / Q000097:blood / Q000172:deficiency
chemical_list: D000090:Acetoacetates; D002951:Citrates; D006885:Hydroxybutyrates; D007656:Ketoglutaric Acids; D007773:Lactates; D011773:Pyruvates; D019343:Citric Acid; D012694:Serine; C016635:acetoacetic acid; D019289:Pyruvic Acid; D005998:Glycine; D020155:3-Hydroxybutyric Acid