Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells.
A cDNA sequence coding for a human granulocyte-macrophage colony-stimulating factor has been isolated from cDNA libraries prepared from mRNA derived from concanavalin A-activated human T-cell clones. The libraries constructed in the pcD vector system were screened by transfecting COS-7 monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the cell supernatants, we identified clones encoding a factor that stimulates the formation of granulocyte and macrophage colonies from human progenitor cells. These results demonstrate that identification of full-length cDNAs for many colony-stimulating factors may be achieved entirely on the basis of detecting the functional polypeptide produced in mammalian cells.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000187:drug effects; D002999:Clone Cells / Q000032:analysis; D003115:Colony-Stimulating Factors / Q000235:genetics* / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000302:isolation & purification*; D005786:Gene Expression Regulation / Q000187:drug effects; D006098:Granulocytes / Q000166:cytology; D000882:Haplorhini; D006801:Humans; D008213:Lymphocyte Activation; D008264:Macrophages / Q000166:cytology; D051379:Mice; D009693:Nucleic Acid Hybridization; D013601:T-Lymphocytes / Q000187:drug effects; D013604:T-Phages / Q000235:genetics; D014162:Transfection
D003115:Colony-Stimulating Factors; D003208:Concanavalin A; D004247:DNA