Interferon production in BCG-sensitized rabbits challenged with PPD.
Intravenous administration of PPD into BCG-primed rabbits results in the release of IFN into the circulation. Peak interferon titres occur in plasma 1 h after PPD injection, rapidly decline during the following 3 h and depend on the dose of tuberculin administered. Bilateral nephrectomy of BCG-primed rabbits challenged with PPD affects remarkably the disappearance of IFN from the circulation. Characterization of the BCG-PPD-induced IFN shows that it is heat-labile, is almost completely inactivated after dialysis at pH 2 and displays a remarkable cross-species activity, particularly on human cell lines.
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000097:blood; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009392:Nephrectomy; D011817:Rabbits; D013154:Spleen / Q000378:metabolism; D013997:Time Factors; D014373:Tuberculin / Q000276:immunology*; D014611:Vaccination
D001500:BCG Vaccine; D014373:Tuberculin; D007371:Interferon-gamma