title: A gene controlling segregation of the Bacillus subtilis plasmid pC194.
abstract: Plasmid pC194-1, a mutant of pC194, and chimeric derivatives of pC194-1 are segregationally unstable in B. subtilis. Such instability could be enhanced by exposure of pC194-1-carrying cells to methyl methanesulfonate. pC194-1 is distinct from pC194 in the addition of two A:T base pairs within the previously defined D region of pC194. Complementation experiments between pC194-1 and other plasmids suggest that the mutation of pC194-1 interferes with the production of a diffusible gene product required for plasmid maintenance.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects; D011995:Recombination, Genetic; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
chemical_list: D008741:Methyl Methanesulfonate