title: Dephosphorylation of thiamin pyrophosphate by fresh human plasma.
abstract: Thiamin pyrophosphate was added to human plasma and serum. After various incubations, aliquots were assayed by the thiochrome method. This revealed that fresh human plasma dephosphorylated thiamin pyrophosphate by a reaction that was time and temperature dependent and inhibited by the presence of ethylenediaminetetraacetic acid (EDTA). Fresh plasma did not dephosphorylate pyridoxal-5-phosphate nor the protein bound thiamin pyrophosphate of red cells.
mesh_terms: D004492:Edetic Acid / Q000494:pharmacology; D004952:Esters / Q000097:blood; D006801:Humans; D010949:Plasma / Q000378:metabolism; D011485:Protein Binding; D013048:Specimen Handling; D013696:Temperature; D013835:Thiamine Pyrophosphate / Q000097:blood*; D013997:Time Factors
chemical_list: D004952:Esters; D004492:Edetic Acid; D013835:Thiamine Pyrophosphate