title: Ability of polyvalent (Crotalidae) antivenin to neutralize myonecrosis, hemorrhage and lethality induced by timber rattlesnake (Crotalus horridus horridus) venom.
abstract: The local and lethal effects of crude Crotalus horridus horridus venom were quantitated and the ability of polyvalent (Crotalidae) antivenin to neutralize these effects was studied. A light microscopy assay was used to measure myonecrosis, a new method based on the amount of hemoglobin present in the injected muscle was used to measure hemorrhage and the LD50 was used as a measure of lethality. Results indicate that polyvalent (Crotalidae) antivenin significantly neutralized myonecrosis in mice at venom doses of 3.0 and 6.0 micrograms/g, hemorrhage at doses of venom up to 12.0 micrograms/g, and lethality at a dose equivalent to 2.5 times the LD50.
mesh_terms: D000818:Animals; D000997:Antivenins / Q000627:therapeutic use*; D003435:Crotalid Venoms / Q000032:analysis / Q000633:toxicity*; D005260:Female; D006470:Hemorrhage / Q000517:prevention & control*; D005779:Immunodiffusion; D007928:Lethal Dose 50; D051379:Mice; D009135:Muscular Diseases / Q000473:pathology / Q000517:prevention & control*; D009336:Necrosis
chemical_list: D000997:Antivenins; D003435:Crotalid Venoms