Mutation to herbicide resistance maps within the psbA gene of Anacystis nidulans R2.
A psbA gene encoding the target of photosystem II herbicide inhibition, the 32,000-dalton thylakoid membrane protein, has been cloned from a mutant of Anacystis nidulans R2, which is resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea-(diuron). A cloned DNA fragment from within the coding region of this gene transforms wild-type cells to herbicide resistance, proving that mutation within psbA is responsible for that phenotype. The mutation consists of a single nucleotide change that replaces serine at position 264 of the wild-type protein with alanine in that of the diuron-resistant mutant.
D000595:Amino Acid Sequence; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics*; D004237:Diuron; D004351:Drug Resistance; D004591:Electrophoresis, Polyacrylamide Gel; D006540:Herbicides; D008565:Membrane Proteins / Q000235:genetics; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype
D006540:Herbicides; D008565:Membrane Proteins; D004237:Diuron