Distribution of GABA binding site subtypes in rat pituitary gland.
GABAA and GABAB binding sites in rat pituitary gland were investigated using equilibrium binding assays in vitro. Specific binding of both [3H]GABA and [3H]muscimol could be detected in both anterior and neurointermediate lobes, with a relative concentration in the anterior lobe. [3H]GABA binding was discriminated into GABAA and GABAB receptor type binding using baclofen. GABAB sites were detectable in the anterior but not in the neurointermediate lobe. Saturation analysis of [3H]muscimol binding to whole pituitary gland membranes demonstrated that the pituitary contains two classes of GABAA sites differing in affinity, as found in the CNS, although the number of sites is considerably lower than in the CNS.
D000818:Animals; D001665:Binding Sites; D002531:Cerebellum / Q000378:metabolism; D005625:Frontal Lobe / Q000378:metabolism; D008297:Male; D009118:Muscimol / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D010904:Pituitary Gland, Posterior / Q000378:metabolism; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D009118:Muscimol; D005680:gamma-Aminobutyric Acid