Stimulation of intestinal adenylate cyclase by cholera toxin in malnourished rats.
The stimulation of intestinal adenylate cyclase by cholera toxin (CT) was studied in normal and malnourished rats 4 to 24 hr after a 30-min incubation of intestinal loops with the toxin. Whereas in control rats the enzyme activity returned to basal levels after 12 hr of incubation, in malnourished rats the activity of the enzyme remained significantly elevated even after 24 hr of the initial incubation. Malnourished animals had a reduced turnover rate of intestinal cells as determined by thymidine kinase activity. The delayed turnover of intoxicated cells may account for continuous activation of mucosal adenylate cyclase and possibly for prolongation of diarrhea in malnutrition.
D000262:Adenylyl Cyclases / Q000032:analysis*; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D001835:Body Weight; D002455:Cell Division; D002772:Cholera Toxin / Q000494:pharmacology*; D003967:Diarrhea / Q000150:complications / Q000201:enzymology; D004768:Enterotoxins / Q000494:pharmacology; D029968:Escherichia coli Proteins; D005260:Female; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D011502:Protein-Energy Malnutrition / Q000150:complications / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013393:Sucrase / Q000032:analysis; D013937:Thymidine Kinase / Q000032:analysis
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli; D013937:Thymidine Kinase; D013393:Sucrase; D000262:Adenylyl Cyclases