A fluorescence staining method for the demonstration and measurement of lysosomal enzyme activities in single cells.
A cytochemical fluorescence method is described that makes possible simple, rapid, and specific demonstration and measurement of the activities of a wide variety of lysosomal enzymes in single cells using 4-methylumbelliferyl derivatives as substrates. The validity of the method and a number of applications using normal and mutant human cells are presented.
D005347:Fibroblasts / Q000201:enzymology; D005470:Fluorometry; D006596:Hexosaminidases / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008661:Metabolism, Inborn Errors / Q000175:diagnosis; D008722:Methods; D001616:beta-Galactosidase / Q000032:analysis
D006596:Hexosaminidases; D001616:beta-Galactosidase