Use of [3H]raffinose as a specific probe of autophagic sequestration.
The trisaccharide [3H]raffinose, introduced into the cytosol of isolated rat hepatocytes by means of electropermeabilization, was sequestered autophagically and accumulated in lysosomes and pre-lysosomal vacuoles. In contrast to the disaccharide [14C]sucrose previously used as a sequestration probe, raffinose was not taken up by the mitochondria. The sequestration of raffinose was completely inhibited by the autophagy suppressor 3-methyladenine, stressing its potential utility as a specific probe of hepatocytic autophagy.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy / Q000187:drug effects; D004072:Digitonin; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects; D010588:Phagosomes / Q000378:metabolism; D011887:Raffinose / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose / Q000378:metabolism
D009844:Oligosaccharides; C025946:3-methyladenine; D013395:Sucrose; D000225:Adenine; D004072:Digitonin; D011887:Raffinose