Transformation and mobilization of cloning vectors in Acinetobacter spp.
R300B-, RSF1010-, and RK2-derived plasmids were introduced into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by transformation and conjugal mobilization. The transformation frequencies of BD413 were 4.2 X 10(6) to 6.3 X 10(6) transformants per micrograms of DNA per 10(9) recipient cells. Conjugal mobilization frequencies were 1.1 X 10(-1) to 8.5 X 10(-1) per recipient. An improved method for the transformation of A. calcoaceticus BD413 is reported.
D000150:Acinetobacter / Q000235:genetics*; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D005822:Genetic Vectors; D010957:Plasmids; D014169:Transformation, Bacterial