A possible role for polyamines in cartilage in the mechanism of calcification.
The role of polyamines in cartilage is not known: they may be somehow related to the mechanism of calcification. In epiphyseal cartilage from calf scapulas, they are more concentrated in the ossifying area, where calcification takes place, than in the resting region. Spermidine is present in greater amounts than spermine and putrescine. Since ornithine decarboxylase (EC 4.1.1.17) is measurable only in the resting region of the tissue, it is in this area that polyamine biosynthesis occurs, while they accumulate in the ossifying area. Immunohistochemical evidence is obtained that only in the ossifying zone is spermidine extracellular. It is at this level that the matrix is rearranged to become calcified, and proteoglycans are dissociated and partially removed. The effect of polyamines on solutions of proteoglycan subunits has been studied in vitro by following variations of turbidity and viscosity. While in the presence of putrescine the specific viscosity decreases to asymptotic values, in the presence of either 30 mM spermidine or 2.5-10 mM spermine, the decrement is more marked. At the same concentrations, increase of the turbidity of proteoglycan subunit solutions was observed. Only spermidine showed the capacity of displacing proteoglycan subunits from a column of Sepharose 4B-type II collagen: at 15 mM concentration, about 90% of proteoglycans were removed from the column. Alkaline phosphatase activity, which plays an important role in calcification, is enhanced by spermidine and spermine. These results obtained in vitro support the hypothesis that polyamines may be related to calcification of preosseous cartilage.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic; D002356:Cartilage / Q000032:analysis / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000502:physiology; D010012:Osteogenesis; D011509:Proteoglycans / Q000502:physiology; D011700:Putrescine / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D012540:Scapula; D013095:Spermidine / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D013096:Spermine / Q000032:analysis / Q000378:metabolism / Q000502:physiology*
D011509:Proteoglycans; D013096:Spermine; D003094:Collagen; D000469:Alkaline Phosphatase; D013095:Spermidine; D011700:Putrescine