Measurement of plasma oxalate in healthy subjects and in patients with chronic renal failure using immobilised oxalate oxidase.
A continuous flow assay using immobilised oxalate oxidase was used to measure the level of oxalate in plasma ultrafiltrate obtained from healthy subjects and from patients with chronic renal failure. The levels of oxalate in plasma from normal subjects ranged from 1.3-3.1 mumol/l (mean 2.03; SD = 0.52) with females showing a higher (p less than 0.05) level (mean 2.25 mumol/l) than males (mean 1.87 mumol/l). The mean oxalate/creatinine clearance ratio in fourteen healthy subjects was greater than unity, thus indicating a net tubular secretion of oxalate. At physiological pH, L-ascorbate was converted to oxalate in whole blood following venepuncture, in plasma and in plasma ultrafiltrate. Reduction of the spontaneous generation of oxalate in the samples prior to analysis was achieved by acidification and treatment with sodium nitrite. A linear correlation (r = 0.92; p less than 0.001) was found between plasma oxalate and plasma creatinine in patients with chronic renal failure.
D000328:Adult; D001205:Ascorbic Acid / Q000097:blood; D003404:Creatinine / Q000097:blood / Q000652:urine; D004800:Enzymes, Immobilized; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007676:Kidney Failure, Chronic / Q000097:blood*; D008297:Male; D010070:Oxalates / Q000097:blood* / Q000652:urine; D019815:Oxalic Acid; D010088:Oxidoreductases; D012016:Reference Values; D012737:Sex Factors; D012977:Sodium Nitrite / Q000494:pharmacology; D014462:Ultrafiltration
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D010070:Oxalates; D019815:Oxalic Acid; D003404:Creatinine; D010088:Oxidoreductases; C020598:oxalate oxidase; D012977:Sodium Nitrite; D001205:Ascorbic Acid