title: Heat-shock response in Xenopus oocytes during meiotic maturation and activation.
abstract: After a 60 min heat-shock at 36 degrees C, Xenopus oocytes are still able to accomplish a complete meiotic maturation in response to a progesterone treatment. The 36 degrees C heat-shock applied to maturing oocytes strongly enhances the synthesis of a single heat-shock protein of approx. 70 000 molecular weight (hsp70); after activation with the Ca2+-ionophore A 23187, matured oocytes still display the ability to synthesize hsp70 and to survive a heat-shock. A cycloheximide treatment combined with a heat-shock induces, during the recovery period, the synthesis of two heat-shock proteins, of approx. 70 000 and 83 000 molecular weight.
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008540:Meiosis / Q000187:drug effects*; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology*; D013462:Sulfur Radioisotopes; D014981:Xenopus
chemical_list: D006360:Heat-Shock Proteins; D013462:Sulfur Radioisotopes; D000001:Calcimycin; D011374:Progesterone; D003513:Cycloheximide; D008715:Methionine