Sequence specific antibodies to a deoxyribodinucleotide.
Antibodies were elicited in rabbits to the dinucleotide, dpTpA, conjugated to a carrier protein. Among a few deoxyribomono- and oligonucleotides tested for binding to the antibodies by nitrocellulose tilter binding assay, only dpTpA and dpTpA containing oligonucleotides showed binding. The inhibition of the binding of 3H-dpTpA to the antibodies by various nonradioactive mono- and oligonucleotides showed that the antibodies were sequence specific and recognized the whole molecule of dpTpA. dpTpA specific antibodies were purified by a two step affinity chromatography. By competition studies, it was found that the purified antibodies bound denatured DNA at dpTpA sequences. The antibodies did not bind RNA.
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D009838:Oligodeoxyribonucleotides / Q000032:analysis*; D009841:Oligonucleotides; D012710:Serum Albumin, Bovine; D013329:Structure-Activity Relationship
D000906:Antibodies; D000936:Antigen-Antibody Complex; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; C042899:deoxythymidylyl-3'-5'-deoxyadenylate; D012710:Serum Albumin, Bovine