The anti-chlamydial and anti-proliferative activities of recombinant murine interferon-gamma are not dependent on tryptophan concentrations.
The effect of recombinant murine interferon-gamma on the growth of Chlamydia trachomatis was analyzed in a mouse fibroblast cell line (McCoy cells). Murine interferon-gamma had a very potent anti-chlamydial activity, although minimally affecting cellular proliferation. Over 95% inhibition of chlamydial inclusions was obtained at a concentration of 1 U/ml of interferon. At a concentration of 1 U/ml of murine interferon-gamma, there was minimal inhibition of the proliferative capacity of McCoy cells. Approximately 50% inhibition of cell growth was obtained with a concentration of 10 U/ml of interferon. Varying concentrations of tryptophan in the medium did not alter either the anti-chlamydial or the anti-proliferative activity of the interferon.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002692:Chlamydia trachomatis / Q000187:drug effects*; D003470:Culture Media; D004347:Drug Interactions; D007371:Interferon-gamma / Q000494:pharmacology*; D007739:L Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology*; D014364:Tryptophan / Q000494:pharmacology*
D003470:Culture Media; D011994:Recombinant Proteins; D007371:Interferon-gamma; D014364:Tryptophan