Dopamine sulfoconjugation in the rat brain: regulation by monoamine oxidase.
An increase of free 3,4-dihydroxyphenylethylamine (DA, dopamine) in the rat brain such as is found following 3,4-dihydroxyphenylalanine (L-DOPA) administration or an intraventricular injection of free dopamine did not result in DA sulfate formation, despite the presence of phenolsulfotransferase activity in various regions of the brain and the high affinity of DA for this enzyme. However, when rats were pretreated with pargyline, a monoamine oxidase inhibitor, the same treatment with L-DOPA or free DA led to active synthesis of DA sulfate. The increase in DA sulfate was significantly correlated with the degree of monoamine oxidase inhibition and directly proportional to free DA concentrations in the hypothalamus (r = 0.86), striatum (r = 0.54), and brainstem (r = 0.89). The highest ratio of DA sulfate to free DA was found in the hypothalamus, suggesting that sulfoconjugation is most active in this region. Prior treatment of rats with 6-hydroxydopamine did not decrease DA sulfate concentrations, indicating that sulfoconjugation occurs most likely in extraneuronal tissues not destroyed by the neurotoxin. The results are compatible with the notion that phenolsulfotransferase may be highly compartmentalized and that inhibition of monoamine oxidase allows the newly generated free DA to become accessible to the sulfoconjugating enzyme, resulting in increase in DA sulfation.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects; D001933:Brain Stem / Q000032:analysis; D003342:Corpus Striatum / Q000032:analysis; D004298:Dopamine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006719:Homovanillic Acid / Q000032:analysis; D006892:Hydroxydopamines / Q000494:pharmacology; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D008995:Monoamine Oxidase / Q000502:physiology*; D016627:Oxidopamine; D010293:Pargyline / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D006892:Hydroxydopamines; D015102:3,4-Dihydroxyphenylacetic Acid; C007558:dopamine 3-O-sulfate; D016627:Oxidopamine; D010293:Pargyline; D008995:Monoamine Oxidase; D004298:Dopamine; D006719:Homovanillic Acid