Survival and recovery of human platelets stored for five days in a non-plasma medium.
Human blood platelets were stored for five days as concentrates in 60 mL of: (a) plasma; (b) non-plasma medium with anticoagulant; and (c) non-plasma medium without anticoagulant. All preparations were equally functional when tested for platelet aggregation and release reaction in response to single agonist or synergistic pairs of agonists in vitro. Platelets stored in non-plasma medium with anti-coagulant had lower kallikrein, fibrino(gen)peptide A, lactate, and beta-thromboglobulin than did plasma controls after five days. In vivo recovery and survival of platelets stored in non-plasma medium with anticoagulant were 51.2% +/- 4.3% and 8.7 +/- 0.3 days, respectively, which were not statistically different from plasma controls of 39.2% +/- 4.9% and 7.2 +/- 0.8 days, respectively. It is concluded that platelets can be stored for five days in a non-plasma medium and still have good in vivo recoveries and survivals.
D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D002470:Cell Survival; D003470:Culture Media; D006801:Humans; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D010974:Platelet Aggregation; D013997:Time Factors
D003470:Culture Media; D007773:Lactates; D019344:Lactic Acid