Isolation and sequence analysis of oxytocin from the sheep corpus luteum.
Studies have revealed that the corpus luteum (CL) of the sheep releases oxytocin (OT) -like immunoreactivity under normal and physiological conditions. We have now purified and completely characterized the OT-like species from ovine CL and established by Edman degradation and comparative reverse-phase HPLC its identity with hypothalamic oxytocin. On the basis of radioimmunoassay, the characterized oxytocin was the only peptide possessing OT-like immunoreactivity. This study represents the first identification by sequence analysis of oxytocin outside the central nervous system.
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003338:Corpus Luteum / Q000032:analysis*; D005260:Female; D010121:Oxytocin / Q000138:chemical synthesis / Q000302:isolation & purification*; D011863:Radioimmunoassay; D012756:Sheep
D010121:Oxytocin