Quantitative enzyme-linked immunosorbent assay (ELISA) for hirudin.
A competitive ELISA assay has been developed that permits reproducible quantitation of the anticoagulant hirudin in buffer and urine. Coupling of peroxidase and hirudin was performed with the heterobifunctional reagent N-succinimidyl-3-(2-pyridyldithio)-propionate. In both solvents the lower limit of sensitivity was 8 ng hirudin/ml (0.08 AT-U) while the upper limit was 7.7 micrograms/ml (78.45 AT-U).
D000818:Animals; D002021:Buffers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006629:Hirudins / Q000032:analysis* / Q000652:urine; D008832:Microchemistry; D012756:Sheep / Q000276:immunology
D002021:Buffers; D006629:Hirudins