The effect of gossypol on the frequency of DNA-strand breaks in human leukocytes in vitro.
Gossypol, a human antifertility agent isolated from the cotton plant, was found to induce a dose-dependent increase in the frequency of DNA-strand breaks in human leukocytes exposed to 2-40 micrograms/ml of the drug for 1 h in serum-free medium in vitro. DNA-strand breaks were studied by alkaline elution or alkaline unwinding of DNA followed by hydroxylapatite-chromatography. No decrease of gossypol-induced DNA-strand breaks was observed after post-treatment incubation times up to 24 h, whereas X-ray-induced DNA breaks disappeared within 2 h under the same incubation conditions. Cells exposed to gossypol in the presence of 10% fetal calf serum showed no or little increase of DNA breaks, suggesting that serum proteins inhibit the DNA-damaging activity of the drug. Both optical isomers of gossypol induced DNA-strand breaks. However, the effect of (-)-gossypol was only about half of that of (+)-gossypol and the racemic form. The induction and persistence of DNA-strand breaks by gossypol, as well as the reduction of this effect in the presence of serum should be considered in the evaluation of the potential in vivo genotoxicity of the drug.
D002453:Cell Cycle; D002470:Cell Survival; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D006072:Gossypol / Q000494:pharmacology*; D006801:Humans; D007536:Isomerism; D007962:Leukocytes / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure
D004247:DNA; D006072:Gossypol