Genetic and nongenetic control of the immune response of mice to a synthetic glycolipid, stearylisomaltotetraose.
Evidence for genetic control of antibody response to stearylisomaltotetraose (ST-IM4), a chemically defined synthetic glycolipid, was studied in various mouse strains. Anti-glycolipid antibodies were induced by repeated injections of ST-IM4 in complete Freund's adjuvant. C57BL and CBA/J mice were found to be good responders, while A/J, SJL/J, and AKR/J mice were poor responders. Responsiveness is independent of the H-2 genotype since AKR/J and CBA/J mice share the same H-2 locus. In addition, B10.A and B10.PL mice developed antibody levels similar to those of C57BL. The immunoglobulin heavy-chain locus (IgCH) was also found not to control antibody levels to ST-IM4 since C57BL and SJL/J mice share the same IgCH allotype. In C58/J mice, wide variation in response was observed among individual mice. Study of the response to ST-IM4 in 12 breeder pairs from different family lines of the C58/J colony also indicated that the regulation of the immune response to ST-IM4 is apparently more complex than can be explained by single gene control. C58 mice, unlike many other strains, had very low preimmune titers.
D000818:Animals; D000917:Antibody Formation; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000276:immunology; D007700:Kinetics; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D009844:Oligosaccharides / Q000276:immunology*; D013045:Species Specificity
D006183:H-2 Antigens; D007143:Immunoglobulin Heavy Chains; D009844:Oligosaccharides; C031488:stearyl-isomaltosyl oligosaccharide; D005620:Freund's Adjuvant