The skin tumor-promoter 12-O-tetradecanoylphorbol-13-acetate induces transcription of the c-fos proto-oncogene in human bladder epithelial cells.
The effect of a single treatment with the skin tumor-promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) on the expression of the cellular proto-oncogenes, c-myc, c-rasHa, c-rasKi and c-fos was examined in the non-tumorigenic human bladder epithelial cell line HCV 29. TPA (1 microgram/ml) increased the transcription of the c-fos gene of HCV 29 at least 50-fold, and this stimulation was observed within minutes. The response was transient, and was accompanied by a rapid and transient change in cell morphology. The expression of c-myc, c-rasHa and c-rasKi were not enhanced by the TPA treatment. These results show that human bladder epithelial cells respond to a known skin tumor-promoter, TPA, by altering the transcription of a specific proto-oncogene in these cells.
D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D010704:Phorbols / Q000633:toxicity*; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012878:Skin Neoplasms / Q000139:chemically induced*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D014158:Transcription, Genetic / Q000187:drug effects*; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
C000715470:MAS1 protein, human; D010704:Phorbols; D000090063:Proto-Oncogene Mas; D013755:Tetradecanoylphorbol Acetate