Expression of Drosophila hsp 70-CAT hybrid gene in Aedes cells induced by heat shock.
DNA-mediated transfer of a Drosophila hsp 70-CAT hybrid gene into Drosophila S3 cells leads to the appearance of heat shock (37 degrees C)-inducible chloramphenicol acetyltransferase (CAT) activity. When this hybrid gene construction was transfected into cultured Aedes, Plodia, or Manduca cells, only trace levels of heat-inducible CAT activity were observed. Induction could be somewhat improved by using Schneider's Drosophila medium for transfection. In the case of Aedes cells, levels of CAT induction comparable to that seen using Drosophila cells could be achieved by raising the heat-shock temperature to 41 degrees C, a treatment which is lethal to Drosophila.
D000123:Acetyltransferases / Q000096:biosynthesis* / Q000235:genetics; D000330:Aedes / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D004331:Drosophila melanogaster / Q000201:enzymology*; D004790:Enzyme Induction; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature; D007915:Lepidoptera / Q000378:metabolism; D010957:Plasmids; D013045:Species Specificity; D014170:Transformation, Genetic
D006360:Heat-Shock Proteins; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase