4-Methylumbelliferyl lipase in human and mouse brain: a possible localization in myelin.
4-Methylumbelliferyl (4-MU) lipase activity in human and mouse brain, measured with 4-MU palmitate, was characterized with respect to effects of pH and detergents, and subcellular and myelin localization. Purified myelin isolated by Norton's procedure [J. Neurochem. 21, 749-757 (1983)] contained higher specific activity of 4-MU lipase, particularly in alkaline pH. Myelin lipase activity was markedly affected by the addition of different types of detergents, the amount of detergents added, and substrate. The optimal pH in myelin was bimodal--pH 4.5 and up to 8.0, respectively. These data indicate that myelin possesses 4-MU lipase activity at alkaline pH, with lower levels at acidic pH.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D051379:Mice; D009186:Myelin Sheath / Q000201:enzymology*; D017830:Octoxynol; D010713:Phosphatidylcholines / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D013656:Taurocholic Acid / Q000494:pharmacology
D002793:Cholic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D011136:Polysorbates; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D013656:Taurocholic Acid; D017830:Octoxynol; D008049:Lipase; D019826:Cholic Acid