A simple and rapid nephelometric procedure for fibronectin.
A nephelometric assay (Beckman ICS) for the measurement of plasma fibronectin using commercially available reagents (Boehringer) is described. The assay is simple, rapid and cheap and has a high degree of specificity. The within-run variation was between 4.3 and 7.8%. Only 30 microL of antiserum is used for one measurement, instead of 100 microL used with the turbidimetric method.
D000328:Adult; D005260:Female; D005353:Fibronectins / Q000097:blood*; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods*; D012016:Reference Values
D005353:Fibronectins