Interaction of very low density lipoprotein with chicken oocyte membranes.
The interaction of hen 125I-VLDL (very low density lipoprotein) with chicken oocyte membranes was characterized using a rapid sedimentation assay. Equilibrium and kinetic studies showed an apparent dissociation constant (Kd) 8.7-9.1 x 10(-8) M or 43.5-45.5 micrograms VLDL protein/ml. Binding capacity was 2.0 micrograms VLDL protein/mg membrane homogenate protein. The apparent rate constants were k1 = 2.4 x 10(5) M-1 min-1 and k2 = 2.1 x 10(-2) min-1. Specific binding required the presence of divalent cations. Whereas binding was completely restored after treatment with EDTA by the addition of MN++, only 60% of binding was restored using Ca++.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002413:Cations, Divalent; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D008433:Mathematics; D008954:Models, Biological; D009865:Oocytes / Q000378:metabolism*
D002413:Cations, Divalent; D008079:Lipoproteins, VLDL; D008345:Manganese; D004492:Edetic Acid