Activated human monocytes express the c-sis proto-oncogene and release a mediator showing PDGF-like activity.
Current ideas about the mechanism of wound healing and the pathogenesis of atherosclerosis, pulmonary fibrosis and hepatic fibrosis suggest a central role for the mononuclear phagocyte in attracting and/or stimulating the proliferation of mesenchymal cells. We demonstrate here that activated human blood monocytes, but not resting monocytes, release a mediator that attracts smooth muscle cells and cooperates with other mediators to stimulate fibroblast proliferation. This mediator is very similar to platelet-derived growth factor (PDGF): its chromatographic properties and chemical stability are similar to those of PDGF, it competes with 125I-PDGF for binding to fibroblasts and it immunoprecipitates with anti-PDGF antibodies. In parallel, stimulated monocytes, but not resting monocytes, express the c-sis proto-oncogene, a gene coding for one of the PDGF chains, consistent with the concept that expression of the c-sis proto-oncogene may be involved in the ability of mononuclear phagocytes to modulate the accumulation of mesenchymal cells.
D005786:Gene Expression Regulation; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D015846:Monokines; D010586:Phagocytes / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000502:physiology*; D011506:Proteins / Q000502:physiology; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes
C000715470:MAS1 protein, human; D015846:Monokines; D010982:Platelet-Derived Growth Factor; D011506:Proteins; D000090063:Proto-Oncogene Mas