Influence of 16,16-dimethyl prostaglandin E2 on morphology and brush border enzymes of small-bowel mucosa. Differences in reactivity between adult and suckling rats.
High doses of 16,16-dimethyl prostaglandin E2 (dmPGE) are trophic to the small bowel of adult and suckling rats. In suckling rats this effect is paralleled by an increase in brush border enzyme activities, possibly indicating accelerated mucosal maturation. To investigate the possible physiological significance of this phenomenon, we examined whether this induction of intestinal enzyme activities can be reproduced in adult rats and whether cell growth and enzyme activity might be suppressed by indomethacin. Treatment twice daily for 2 weeks with 100 micrograms/kg dmPGE by intragastric instillation increased villus length in the proximal and distal small bowel by 36% and 40%, respectively, while 2 mg/kg indomethacin by subcutaneous injection had no effect. Maltase, trehalase, lactase, and sucrase activities were unchanged after dmPGE or indomethacin. [3H]-thymidine incorporation into DNA was not significantly influenced for up to 24 h after a single dose of both 100 micrograms/kg PGE intragastrically or 10 mg/kg indomethacin subcutaneously. These studies confirm that in adult rats large doses of 16,16-dm PGE2 increase the volume of the small-bowel mucosa. In contrast to the situation in suckling rats, the activity of hydrolytic brush border enzymes is not increased. There is thus no evidence that endogenous prostaglandins are trophic or influence brush border enzymes in the adult rat.
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology*; D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D002455:Cell Division / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D007421:Intestine, Small / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D011453:Prostaglandins / Q000502:physiology; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013393:Sucrase / Q000378:metabolism; D014198:Trehalase / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D011453:Prostaglandins; D011459:Prostaglandins E, Synthetic; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D014198:Trehalase; D013393:Sucrase; D015064:16,16-Dimethylprostaglandin E2; D007213:Indomethacin