Membrane carbohydrates of lymphoid cells: the receptor for interleukin 2.
The contribution of sialic acids and of N-linked sugars to the biological activity of the receptor for IL 2 has been evaluated by treating activated cells with Neuraminidase or by growing them in the presence of inhibitors of N-linked glycosylation or processing. After treatment with Neuraminidase, Con A-activated spleen cells had not lost their ability to bind IL 2. The IL 2-absorbing capability was, however, strongly reduced after trypsinisation. 6 hours after Trypsin treatment, this property was again expressed. Proliferation of the IL 2-dependent CTLL cells was normal in the presence of Swainsonine but strongly impaired in the presence of Tunicamycin. Glycosylation of the IL 2 receptor may thus be required, but integrity of the sugars is not critical.
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D011971:Receptors, Immunologic / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D015375:Receptors, Interleukin-2; D012794:Sialic Acids / Q000378:metabolism; D017026:Swainsonine; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology
D000470:Alkaloids; D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D012794:Sialic Acids; D014415:Tunicamycin; D017026:Swainsonine