Degenerative changes, DNA synthesis and mitotic activity in rat liver following single exposure to diethylnitrosamine.
Degenerative and regenerative changes induced in rat liver by single exposure to diethylnitrosamine (DEN) were examined by morphological and biochemical approaches. Apoptotic changes were observed in livers of rats exposed to a 'subnecrogenic' dose of DEN (10 mg/kg) as well as in liver parenchyma of those receiving a necrogenic dose (100 mg/kg). Zonal centrilobular necrosis was observed exclusively in the latter group. Regenerative changes, i.e., increases in DNA synthesis, labeling index and mitotic activity, occurred only in animals exposed to the higher dose. The mitogenic effect obtained in these conditions was about half that induced by two-thirds hepatectomy and the maximum response occurred about 24 h later than in partially hepatectomized rats.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D004052:Diethylnitrosamine / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006651:Histocytochemistry; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D008938:Mitosis / Q000187:drug effects*; D009336:Necrosis / Q000139:chemically induced; D051381:Rats; D011919:Rats, Inbred Strains
D004052:Diethylnitrosamine; D004247:DNA