Prostaglandin output from the early pregnant rat uterus superfused in vitro, and the effect of A23187 and trifluoperazine.
The outputs of prostaglandin (PG) E-2 and 6-oxo-PGF-1 alpha from the early pregnant rat uterus superfused in vitro were significantly higher (P less than 0.05) on Day 4 (09:00-10:00 h) and Day 5 (14:00-15:00 h) than on Day 2 (09:00-10:00 h) and Day 5 (14:00-15:00 h). PGF-2 alpha output was significantly higher (P less than 0.05) only on Day 5 (09:00-10:00 h). PGE-2 was the major PG released at all times, although the amounts of PGF-2 alpha and/or 6-oxo-PGF-1 alpha released were often only slightly less. These findings are consistent with uterine PGs having a role in implantation in the rat. A23187 stimulated 6-oxo-PGF-1 alpha output and, except on Day 4 (09:00-10:00 h), PGF-2 alpha output at all times studied. A23187 had little effect on PGE-2 output. The greatest stimulatory effect of A23187 on 6-oxo-PGF-1 alpha and PGF-2 alpha outputs occurred on Day 5 (09:00-10:00 h), which is the day of highest uterine PGH-2 synthetase activity. These increases in response to A23187 were prevented by trifluoperazine (100 microM), a calmodulin antagonist. Trifluoperazine had no inhibitory effect on the high basal output of PGs on Day 5 (09:00-10:00 h), but caused a small increase in uterine PG output.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D015237:Dinoprost; D015232:Dinoprostone; D005260:Female; D009924:Organ Culture Techniques; D010477:Perfusion; D011247:Pregnancy; D011453:Prostaglandins / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014268:Trifluoperazine / Q000494:pharmacology*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D014268:Trifluoperazine; D000001:Calcimycin; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone