Fluorescent staining of living mouse neuromuscular junctions.
A technique for staining living neuromuscular junctions is described and used for electrophysiological recording with both intra- and extra-cellular microelectrodes. This technique may be used on thicker muscles (eg. mouse soleus), has no effect on any of several physiological parameters tested, and should have several useful applications.
D000818:Animals; D002196:Capillaries; D004594:Electrophysiology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D037102:Lectins; D008297:Male; D051379:Mice; D008839:Microelectrodes; D009045:Motor Endplate / Q000033:anatomy & histology; D009132:Muscles / Q000098:blood supply; D009469:Neuromuscular Junction / Q000033:anatomy & histology* / Q000502:physiology / Q000648:ultrastructure; D013569:Synapses / Q000502:physiology; D013861:Thiocyanates; D014909:Wheat Germ Agglutinins
D005452:Fluoresceins; D037102:Lectins; D013861:Thiocyanates; D014909:Wheat Germ Agglutinins; D016650:Fluorescein-5-isothiocyanate