Turbidimetric assay of apolipoprotein A-II.
A turbidimetric assay for the quantification of apolipoprotein A-II in serum is described. Its adaptation for the Cobas Bio Analyser (Hoffmann La Roche) is reported. Regression analysis of apolipoprotein A-II values measured by turbidimetry revealed a good measure of agreement with the data obtained by radial immunodiffusion (RID) (r = 0.90, y = 0.97x + 0.021, n = 100) as well as with data obtained by immunonephelometry (r = 0.838, y = 0.925x + 0.029, n = 34). A variation coefficient of 3.2% (n = 18) was found in relation to the precision in the series and a variation coefficient of 3.5% (n = 36) in relation to day to day precision.
D000906:Antibodies / Q000032:analysis; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A / Q000097:blood* / Q000276:immunology / Q000592:standards; D011232:Chemical Precipitation; D006801:Humans; D005779:Immunodiffusion; D008074:Lipoproteins / Q000097:blood; D008274:Magnesium; D015636:Magnesium Chloride; D009391:Nephelometry and Turbidimetry / Q000379:methods; D010772:Phosphotungstic Acid; D011309:Preservation, Biological; D012016:Reference Values
D000906:Antibodies; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A; D008074:Lipoproteins; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid; D008274:Magnesium