title: Association of tobacco etch virus related RNA with chloroplasts in extracts of infected plants.
abstract: The RNA in various subcellular fractions of tobacco etch virus (TEV) infected tissue was analyzed for the presence of complementary viral RNA, and double-stranded viral RNA by hybridization with 32P-labeled viral RNA or cDNA probes. Although viral RNA was detected in several cellular fractions, the complementary RNA of full-length size was found exclusively associated with fractions containing chloroplasts. Treatment of RNAs with RNase before hybridization suggested that the virus-related complementary RNA was present in double-stranded form.
mesh_terms: D002736:Chloroplasts / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000235:genetics*; D010947:Plants, Toxic; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D014026:Nicotiana / Q000382:microbiology*
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral