title: Calcium control of growth hormone release from chicken pituitary glands in vitro.
abstract: The influence of calcium on the basal and stimulated release of growth hormone (GH) from chicken pituitary glands has been determined in vitro. Basal GH release occurred in Ca2+ deficient media, although it was increased in proportion to the medium Ca2+ concentration. Growth hormone release was stimulated by 10(-7)-10(-9) M thyrotrophin-releasing hormone (TRH), maximal stimulation being observed in the presence of 10(-8) M TRH and 1.5 mM Ca2+. Decreases in the Ca2+ concentration (to 0.75, 0.375, or 0 mM) suppressed the GH response to 10(-8) M TRH, as did increases (to 3.0 and 6.0 mM) in the Ca2+ concentration. These results suggest that GH release in chickens is regulated by Ca2+-dependent mechanisms.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002645:Chickens / Q000502:physiology*; D004533:Egtazic Acid / Q000494:pharmacology; D013006:Growth Hormone / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
chemical_list: D004533:Egtazic Acid; D013973:Thyrotropin-Releasing Hormone; D013006:Growth Hormone; D002118:Calcium