[Detection of the common features in the antigenic structure and the spectra of the phosphoprotein kinase activity proper of the water-soluble fractions of DNA-free nuclear nonhistone proteins from hepatomas and the liver in rats following a single administration of hepatocarcinogens].
By means of the reaction of specific inhibition of precipitation in agarose gel and using the rabbit immune sera against nuclear nonhistone proteins (NHP) in the NHP-DNA complexes isolated from the rat kidney it is shown that the single intraperitoneal injection of hepatocarcinogens, 4-dimethylaminoazobenzene (DAB) or N-nitrosodiethylamine (DEN) induces the appearance of hetero-organic antigens of the kidney nature in the NHP pattern of the liver. These antigens identical, as it proved to be, to the same NHP-antigens from the cells of transplantable rat hepatoma 27 and ascitic Zajdela hepatoma could be found in the NHP pattern of the rat liver during 1 to 12 and 1 to 64 days after the DAB and DEN injection, respectively. In all cases when the hetero-organic NHP-antigens were found the profiles of proper phosphoprotein kinase activity of the fractions of NHP eluted by 0.4-0.5 M NaCl using gradient chromatography on phosphocellulose contained peaks that were not characteristic to the liver NHP but were typical of NHP from the intact rat kidney. In the SDS-PAAG electrophoresis the mentioned fractions form one line with the molecular weight 15000-20000.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000276:immunology*; D004247:DNA / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004052:Diethylnitrosamine; D007668:Kidney / Q000201:enzymology / Q000276:immunology; D008099:Liver / Q000201:enzymology / Q000276:immunology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology / Q000276:immunology*; D008297:Male; D008970:Molecular Weight; D011494:Protein Kinases / Q000032:analysis*; D051381:Rats; D012995:Solubility; D013997:Time Factors; D004124:p-Dimethylaminoazobenzene
D000941:Antigens; D000951:Antigens, Neoplasm; D002868:Chromosomal Proteins, Non-Histone; D004273:DNA, Neoplasm; D004052:Diethylnitrosamine; D004247:DNA; D004124:p-Dimethylaminoazobenzene; D011494:Protein Kinases
Vyiavlenie obshchikh chert v antigennoĭ strukture i spektrakh sobstvennoĭ fosfoproteinkinaznoĭ aktivnosti vodorastvorimykh fraktsiĭ svobodnykh ot DNK iadernykh negistonovykh belkov gepatom i pecheni krys posle odnokratnogo vvedeniia gepatokantserogenov.