Modulation of interleukin 2 release from a primate lymphoid cell line in serum-free and serum-containing media.
The ability to grow a clone of the cell line, MLA144, which is a constitutive producer of interleukin 2 (IL-2), in serum-free medium permitted the study of the direct effect of various agents on cell growth and IL-2 production in a homogeneous population. Bovine serum albumin (BSA) at 4 mg/ml was optimal for cell growth and IL-2 production. Selenium at 10 ng/ml enhanced IL-2 production nearly twofold and lithium at 42 ng/ml also enhanced IL-2 production by nearly twofold. Neither compound at these levels altered cellular proliferation. Two other compounds, iron and zinc, known to be associated with cellular proliferation and/or immunoregulation did not alter IL-2 production. Catalase or horseradish peroxidase was able to substitute for BSA and maintain the long-term growth of the MLA144 clone with only a 30% decrease in the rate of cellular proliferation and a 50% decrease in IL-2 production compared to cells maintained in the serum-free formulation with BSA. Addition of 0.5 mg of BSA to the catalase serum-free formulation increased the production of IL-2 to 70% of that of cells cultured in the BSA-containing serum-free formulation. The catalase-containing serum-free formulation has the advantage of consisting of only three proteins, catalase, insulin, and transferrin, at a very low protein content. The catalase-containing serum-free medium also supported the long-term growth of a human T-cell line, HSB-2.
D000818:Animals; D001790:Blood Physiological Phenomena; D002143:Callitrichinae; D002374:Catalase / Q000494:pharmacology; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D012643:Selenium / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D003470:Culture Media; D007376:Interleukin-2; D012710:Serum Albumin, Bovine; D008094:Lithium; D007501:Iron; D002374:Catalase; D012643:Selenium; D015032:Zinc