DNA degradation after interaction of cis- and trans-diamminedichloroplatinum (II) with calf thymus nuclei.
DNA samples isolated from control nuclei and nuclei treated by cis- and trans-diamminedichloroplatinum (DDP) were analyzed by gel electrophoresis. There were no changes in Mr when DNA isolated from nuclei treated with trans-DDP was analyzed. Scans of DNA isolated from nuclei treated with cis-DDP revealed significant changes in Mr. This DNA bears, however, no signs of regular fragmentation. The possible involvement of endonuclease activity in the degradation process is discussed.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism*; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007536:Isomerism; D013950:Thymus Gland / Q000378:metabolism*
C034697:transplatin; D004247:DNA; D002945:Cisplatin