H-2-restricted cytolysis of L cells doubly transformed with a cloned H-2Kb gene and cloned retroviral DNA.
We report the construction of target cells by the double transformation of mouse L cells with a cloned H-2Kb gene and molecular clones of Akv or Gross murine leukemia virus (MuLV) or the cloned gag gene of Akv MuLV. Cytolytic T lymphocytes (CTL) generated in BALB.B mice specific for Gross MuLV (closely related to Akv MuLV) kill these doubly transformed cells. This is a direct indication that a CTL subpopulation recognizes H-2Kb antigen in association with a viral antigen encoded by the gag gene of Gross MuLV.
D000406:AKR murine leukemia virus / Q000235:genetics* / Q000276:immunology; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D003602:Cytotoxicity, Immunologic; D004279:DNA, Viral / Q000378:metabolism; D015683:Gene Products, gag; D005802:Genes, MHC Class II; D005814:Genes, Viral; D006183:H-2 Antigens / Q000235:genetics*; D064532:Histocompatibility Antigen H-2D; D007739:L Cells / Q000145:classification / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D012191:Retroviridae Proteins / Q000235:genetics / Q000276:immunology; D014162:Transfection
D004279:DNA, Viral; D015683:Gene Products, gag; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D012191:Retroviridae Proteins