[Simultaneous immunocytochemical demonstration of the centrosphere and nucleolar organizer in cultured human cells].
A rabbit showing natural centrosphere-immunoreactivity was immunized with streptococcus pyogenes type 24 (S 24). During the immunization, an additional NOR-immunoreactivity appeared. This reactivity was not inhibited after the serum absorption by S 24 and seems fortuitous. The simultaneous immunoperoxidase technique staining centrosphere and NOR is described and illustrated during successive phases of mitosis. Unique as of this date, this serum may serve as a useful tool to study the centrosphere and NOR.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002502:Centrioles / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007399:Interphase; D008938:Mitosis; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D009940:Organoids / Q000648:ultrastructure*; D011506:Proteins / Q000032:analysis
D011506:Proteins
Mise en évidence immunocytochimique simultanée de la centrosphére et de l'organisateur nucléolaire (NOR) dans des cellules humaines en culture.