Separation of naturally occurring or induced methylated nucleobases of DNA by reversed-phase high-performance liquid chromatography.
Separation of the major bases of DNA along with seven minor methylated ones was obtained by reversed-phase high-performance liquid chromatography using an isocratic elution system. Application of this procedure to DNA treated in vitro by N-methyl-N-nitrosourea has allowed identification of two induced minor bases; a third one was resolved using a slightly modified mobile phase. Baseline resolution of 3-methyl- and 5-methylcytosine, detected in Euglena DNA hydrolyzates, was also achieved.
D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004247:DNA / Q000032:analysis*; D005056:Euglena gracilis / Q000378:metabolism; D006868:Hydrolysis; D008770:Methylnitrosourea / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D013950:Thymus Gland / Q000032:analysis / Q000378:metabolism
C036386:3-methylcytosine; C025946:3-methyladenine; D008770:Methylnitrosourea; D003596:Cytosine; D004247:DNA; D000225:Adenine