[Comparison of different methods of introducing a label into the DNA of microorganisms].
Different methods of isotope introduction into plasmid and chromosomal DNA have been compared. The efficiency of label introduction into DNA was estimated by the results of DNA--DNA hybridisation and by the thermostability of hybrid duplexes. Using the enzymatic methods of label introduction levels of DNA specific radioactivity and label binding in homologous and heterologous reactions were close. DNA labeled by the method of enzymatic methylation forms more thermostable hybrid duplexes than "nick-translation" DNA. The advantages of enzymatic methods of label introduction for creating a microorganism reference DNA bank are discussed.
D001412:Bacillus subtilis / Q000235:genetics; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D007553:Isotope Labeling / Q000379:methods*; D008745:Methylation; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012015:Reference Standards; D014316:Tritium
D004269:DNA, Bacterial; D009692:Nucleic Acid Heteroduplexes; D014316:Tritium
Sravnenie razlichnykh sposobov vvedeniia metki v DNK mikroorganizmov.