Comparison of five methods for the analysis of the light chain type of monoclonal serum IgM proteins.
Five methods for the analysis of the light chain type of monoclonal IgM proteins present in serum at low concentration were compared in terms of sensitivity, feasibility for use in a clinical laboratory, technical effort required, and cost. These studies indicated that methods based on the removal of IgG from serum were the most appropriate for routine use.
D000911:Antibodies, Monoclonal / Q000032:analysis*; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D013205:Staphylococcal Protein A
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A