A murine cell line (2E10.4.13) produces five hemopoietic stimulators, and interleukin-2 and interleukin-3.
An interleukin-2 (IL-2)-independent murine lymphocyte clone (2E10.4.13) with the Thy1+Lyt1+2-T200+ phenotype was separated from the original IL-2-dependent natural killer (NK) cell line (PEC-1). Erythroid burst-promoting activity (BPA), erythropoietin (Ep), granulocyte/macrophage, megakaryocyte and eosinophil colony-stimulating factors (GM-, MK- and Eo-CSF), IL-2 and Interleukin-3 (IL-3) were produced when these cells were stimulated with phorbol myristate acetate (PMA). When the conditioned medium was run through ion-exchange high-performance liquid chromatography, BPA, Ep, GM-CSF, MK-CSF and Eo-CSF were eluted in the same region as IL-3. In contrast, MK-CSF, much of the GM-CSF and half of the Eo-CSF were eluted in a distinct region where no IL-3 was detected. Chemical analyses of the hemopoietic factors derived from a single T inducer clone indicated that all the hemopoietic activities were associated with IL-3 activity. Some CSF activities (GM-, MK- and Eo-CSF) also could be mediated by the distinct molecules from IL-3, evidence that heterogeneous molecules are responsible for CSF activity.
D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D004921:Erythropoietin / Q000096:biosynthesis; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D007376:Interleukin-2 / Q000096:biosynthesis*; D007377:Interleukin-3; D008214:Lymphocytes / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D007377:Interleukin-3; D008222:Lymphokines; D004921:Erythropoietin; D013755:Tetradecanoylphorbol Acetate