Rapid enzyme immunoassay for quantification of C-reactive protein (CRP).
A simple, rapid enzyme immunoassay (EMIT CRP assay) for C-reactive protein (CRP) was assessed using human acute phase sera and purified human CRP. The calibration curve was established from 10 micrograms/mL to 150 micrograms/mL. The precision evaluations of the assay, carried out under optimal conditions of the test, (CRP levels: 20-100 micrograms/mL) showed coefficients of variation less than 7%, both for within-run and day-to-day precision. The limit of sensitivity was 10 micrograms/mL. The coefficients of correlation with radial immunodiffusion and immunonephelometry were respectively 0.988 and 0.956. The dilution assay showed a linear response. No significant changes in the CRP level were observed after sample storage either at 4 degrees C or -20 degrees C for 3 to 10 days, or after removing lipoproteins from sera. The EMIT CRP assay is characterized by its short assay time, its excellent precision, and its sensitivity is sufficient to detect an ongoing inflammatory process.
D002097:C-Reactive Protein / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D005779:Immunodiffusion / Q000379:methods; D007124:Immunoenzyme Techniques; D008074:Lipoproteins / Q000097:blood; D009391:Nephelometry and Turbidimetry / Q000379:methods
D008074:Lipoproteins; D002097:C-Reactive Protein