Glucuronidation of bile acids in human liver, intestine and kidney. An in vitro study on hyodeoxycholic acid.
The activities of UDP-glucuronyl transferase(s) in homogenates and microsomal preparations of human liver, kidney and intestine were tested with hyodeoxycholic acid (HDC). The various kinetic parameters of the UDC-glucuronidation were determined from time course experiments. In both liver and kidney preparations, HDC underwent a very active metabolic transformation: liver Km = 78 microM, Vmax = 3.3 nmol . min-1 . mg-1 protein; kidney Km = 186 microM, Vmax = 9.9 nmol . min-1 . mg-1 protein. To our knowledge this is the first observation of both an extensive and comparable bile acid glucuronidation occurring in renal and hepatic tissues.
D001647:Bile Acids and Salts / Q000378:metabolism*; D001706:Biopsy; D002635:Chenodeoxycholic Acid / Q000378:metabolism; D003840:Deoxycholic Acid / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D013997:Time Factors
D001647:Bile Acids and Salts; D005965:Glucuronates; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; C010471:hyodeoxycholic acid; D014453:Glucuronosyltransferase