Some contributions to the thin-layer chromatographic analysis of complex natural phospholipid and neutral lipid mixtures.
We have isolated a minor phosphatidyl ethanolamine component from pancreas and a minor phosphatidyl choline component from retina, which were revealed by their separate thin-layer chromatographic properties on silica gel and aluminum oxide sheets, respectively. We have described in some detail a number of modifications in thin-layer chromatography methodology which enhances the opportunity for assessing the glycerophospholipid and neutral lipid composition of tissues, as attested by a diverse set of examples, and have pointed out some of the associated technical problems.
D000537:Aluminum Oxide; D000818:Animals; D002415:Cats; D002417:Cattle; D002855:Chromatography, Thin Layer; D003720:Densitometry; D005123:Eye / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D009206:Myocardium / Q000032:analysis; D010179:Pancreas / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D010955:Plasmalogens / Q000032:analysis; D011817:Rabbits; D012639:Seeds / Q000032:analysis; D013045:Species Specificity; D013552:Swine; D014426:Turtles
D008055:Lipids; D010743:Phospholipids; D010955:Plasmalogens; D000537:Aluminum Oxide