Solubilization of human sperm antigens.
Various methods of solubilization of human sperm antigens were compared by testing the sperm extracts against rabbit antihuman spermatozoa immune serums with double immunodiffusion and rocket immunoelectrophoresis. Marked differences were found in the protein contents and the SDS-PAGE protein patterns of the extracts. Lithium diiodosalicylate was the most efficient in solubilizing the sperm proteins and in preserving their antigenicity. Nonionic detergents also gave, either alone or combined to sonification and 1.0 M KCl, 24-28 clearly discernible protein bands and several different immunoprecipitates. Ionic detergents deoxycholate and cetylpyridinium chloride solubilized a high yield of proteins, but their antigenity was poor.
D000818:Animals; D000954:Antigens, Surface / Q000302:isolation & purification*; D002594:Cetylpyridinium / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis / Q000379:methods; D007463:Iodobenzoates; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D012459:Salicylates / Q000494:pharmacology; D012995:Solubility; D013094:Spermatozoa / Q000032:analysis / Q000166:cytology* / Q000276:immunology
D000954:Antigens, Surface; D003902:Detergents; D007463:Iodobenzoates; D012459:Salicylates; D003840:Deoxycholic Acid; C020891:3,5-diiodosalicylic acid; D011092:Polyethylene Glycols; D008623:Mercaptoethanol; D017830:Octoxynol; D002594:Cetylpyridinium