The role of complement in cigarette smoke-induced chemotactic activity of lung fluids.
To help explain increased recruitment of leukocytes to smokers' lungs, we examined the possibility that the smoke-induced chemotactic activity in lung fluids may be partly complement-dependent. Acute inhalation exposure of rats to smoke from 2R1 Kentucky Reference cigarettes caused an increase in the leukocyte chemotactic activity of their lung secretions (collected by bronchoalveolar lavage 1 h after exposure). Prior depletion of circulating complement, by intravenous administration of purified cobra venom factor 24 h beforehand, prevented the smoke-induced rise in lavage fluid chemotactic activity. Crossed immunoelectrophoretic analysis of lavage C3 from smoke-exposed animals demonstrated a modified form of the molecule with increased electrophoretic (anodal) mobility. When 2 strains of mice, C5-deficient DBA/2 and C5-sufficient BALB/c, were acutely exposed to cigarette smoke, only the C5-sufficient animals (BALB/c) had increased neutrophil chemotactic activity in their lung fluids 1 h after exposure. These results show that the increased chemotactic activity in lung fluids of laboratory animals immediately after acute cigarette smoke exposure is complement-dependent.
D000818:Animals; D001826:Body Fluids / Q000032:analysis / Q000502:physiology*; D002630:Chemotactic Factors / Q000032:analysis / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D003176:Complement C3 / Q000032:analysis; D003182:Complement C5 / Q000172:deficiency; D003170:Complement Pathway, Alternative / Q000187:drug effects; D003165:Complement System Proteins / Q000502:physiology*; D004546:Elapid Venoms / Q000494:pharmacology; D007249:Inflammation; D008168:Lung / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains; D009504:Neutrophils; D051381:Rats; D011919:Rats, Inbred Strains; D012907:Smoking; D007507:Therapeutic Irrigation
D002630:Chemotactic Factors; D003176:Complement C3; D003182:Complement C5; D004546:Elapid Venoms; C016310:cobra venom factor; D003165:Complement System Proteins