Stimulation of bile-pancreatic zinc, protein and carboxypeptidase secretion in response to various proteins in the rat.
The objective of this study was to determine the rate of bile-pancreatic secretion of zinc, protein and carboxypeptidase (CP) in response to three different dietary proteins, egg white (EW), soy protein isolate (SPI) and casein. The infusion of protein into the duodenum stimulated a significant increase in the secretion rate of zinc, protein and CP in bile-pancreatic juice. There was a high degree of correlation between the concentration of zinc and enzyme activity (CPA and CPB) in bile-pancreatic juice indicating that zinc in bile-pancreatic juice is associated with CP activity. The average secretion rate of zinc, protein and CP during the 1-h period after infusion of protein was significantly greater after SPI infusion than after casein. The bile-pancreatic secretion rate of protein, zinc and CPB in response to EW infusion was consistently intermediate between SPI and casein and did not differ statistically from either. Only CPA secretion rate was significantly greater after EW than after casein. Differences in protein digestibility appear to account for the greater pancreatic response to SPI, and this factor may be important in understanding the effect of protein source on zinc bioavailability.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002268:Carboxypeptidases / Q000378:metabolism*; D002364:Caseins / Q000494:pharmacology; D004044:Dietary Proteins / Q000494:pharmacology*; D004386:Duodenum / Q000378:metabolism; D008297:Male; D009753:Nutritive Value; D010047:Ovalbumin / Q000494:pharmacology; D010179:Pancreas / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism*; D014674:Plant Proteins, Dietary / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012636:Secretory Rate; D030262:Soybean Proteins; D013025:Glycine max; D013268:Stimulation, Chemical; D013997:Time Factors; D015032:Zinc / Q000378:metabolism*
D002364:Caseins; D004044:Dietary Proteins; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins; D010047:Ovalbumin; D002268:Carboxypeptidases; D015032:Zinc