Ca2+ inhibition of brush border alkaline phosphatase activity in Hymenolepis diminuta.
The brush border membrane of Hymenolepis diminuta contains several Ca2+-dependent enzymes. Following our isolation of a Ca2+-dependent modulator protein we examined the kinetic properties of the brush border marker alkaline phosphatase from fractionated and crude tegument. We show that this enzyme is inhibited by Ca2+ concentrations approaching those in the calcareous corpuscles of H. diminuta.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D006926:Hymenolepis / Q000187:drug effects / Q000201:enzymology*; D007422:Intestines / Q000469:parasitology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology*; D051381:Rats
D004533:Egtazic Acid; D000469:Alkaline Phosphatase; D008274:Magnesium; D002118:Calcium