Cytofluorometric determination of nuclear DNA in plant cells using auramine O.
The effect of DNA hydrolysis, staining time, dye concentration and some other important factors was evaluated on measurement of nuclear DNA content in plant cells by cytofluorometry. Based on the results optimal conditions for making permanent Auramine O--SO2 stained squash preparations can be chosen.
D001576:Benzophenoneidum; D002467:Cell Nucleus / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D059828:Plant Cells
D001576:Benzophenoneidum; D004247:DNA