Phosphorylation and protein synthetic events in Xenopus laevis oocytes microinjected with pp60v-src.
Microinjection of purified pp60v-src, the transforming protein of Rous sarcoma virus, into Xenopus laevis oocytes accelerated the rate of progesterone- or insulin-induced meiotic maturation. This acceleration was abolished by incubating the oocytes with cycloheximide or puromycin during a 2-h interval between pp60v-src microinjection and progesterone addition. In contrast, exposure to actinomycin D did not alter the acceleration of maturation by microinjected pp60v-src. Associated with progesterone treatment and pp60v-src microinjection were a number of qualitative changes in phosphoproteins; a few of these changes are common to both stimuli. These results indicate that the action of pp60v-src in oocytes involves both phosphorylation and protein synthetic events that affect oocyte maturation.
D000818:Animals; D007328:Insulin / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D008845:Microinjections; D015688:Oncogene Protein pp60(v-src); D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation; D011374:Progesterone / Q000494:pharmacology; D014176:Protein Biosynthesis; D012191:Retroviridae Proteins / Q000494:pharmacology*; D014982:Xenopus laevis
D007328:Insulin; D012191:Retroviridae Proteins; D011374:Progesterone; D015688:Oncogene Protein pp60(v-src)