Induction of the synthesis of triton-soluble proteins in human keratinocytes by gamma interferon.
Recombinant human gamma interferon (r-IFN-gamma) induces the synthesis and expression of HLA-DR antigen on cultured, normal, human keratinocytes depleted of Langerhans cells. After removal of r-IFN-gamma from the culture medium of keratinocytes that are expressing HLA-DR antigen, the cells continue to express this antigen for at least 2 days. r-IFN-gamma induces, in a dose dependent fashion, the synthesis of several triton-soluble proteins with the most prominent having an apparent molecular weight of 53,000. Whereas normal keratinocytes do not express HLA-DR antigen in vivo, they do express HLA-DR in a variety of skin diseases such as lichen planus, graft-versus-host disease, and mycosis fungoides. We propose that an understanding of lymphocyte-keratinocyte interactions in vivo may be achieved by further studies of the mechanism of action of r-IFN-gamma on cultured keratinocytes and that the results may provide insight into the patho-physiology leading to a number of common inflammatory and neoplastic skin diseases.
D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology*; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology*; D011092:Polyethylene Glycols / Q000502:physiology; D014176:Protein Biosynthesis; D012995:Solubility
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D011092:Polyethylene Glycols; D007371:Interferon-gamma