A new and specific enzyme-linked immunosorbent assay for the detection of C3 nephritic factor.
C3 nephritic factor (C3 NeF) was measured by assessing its capacity to form complex with C3 and B using an enzyme-linked immunosorbent assay (ELISA). Incubation of C3 NeF with normal human serum in the presence of MgEGTA resulted in a dose-dependent increase of C3-B-IgG complex. No complex was formed in EDTA. The C3 NeF titer estimated in this way was in good accordance with those reported previously by other indirect methods.
D003176:Complement C3 / Q000276:immunology; D003178:Complement C3 Nephritic Factor / Q000097:blood*; D003169:Complement Inactivator Proteins / Q000097:blood*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005921:Glomerulonephritis / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology
D003176:Complement C3; D003178:Complement C3 Nephritic Factor; D003169:Complement Inactivator Proteins; D007074:Immunoglobulin G; D004533:Egtazic Acid; D004492:Edetic Acid