Rejection of syngeneic tumor cells by the interaction of Lyt-1+ T lymphocytes and macrophages.
A high degree of cytostatic activity was detected in peritoneal exudate cells (PEC) from BALB/c mice immunized with mitomycin C-treated Meth A cells (MMC-MA) given intraperitoneally (i.p.) While this cytostatic activity was not detected in a nonadherent fraction of immune PEC, the addition of starch-induced PEC to the nonadherent immune cells led to a cytostatic activity. Treatment of these nonadherent cells with anti-Thy-1 X 2 or anti-Lyt-1 X 2 antibody plus complement abolished the restored cytostatic activity. Normal PEC were also rendered cytostatic after addition of the culture supernate of a nonadherent fraction of immune PEC mixed with MMC-MA. These findings suggest that macrophages are rendered cytostatic by immunization with tumor antigen and that these cytostatic macrophages evolve after activation of sensitized Lyt-1 positive T lymphocytes.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000950:Antigens, Ly / Q000276:immunology*; D006084:Graft Rejection; D007518:Isoantibodies / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D007518:Isoantibodies; C029805:Lyt antibodies; D008937:Mitomycins; D016685:Mitomycin; D008748:Methylcholanthrene