Detection and quantitation of aflatoxin B1 in orange juice by SOS-Chromotest.
A new method for the detection and quantitation of aflatoxin B1 in liquids is described. The method is based on the SOS Chromotest, in which damage caused by aflatoxin B1 to the DNA of suitably engineered E. coli induces beta-galactosidase. Aflatoxin B1 developing in orange juice inoculated with spores of Aspergillus parasiticus is detectable equally well by TLC as by the SOS-Chromotest.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D001230:Aspergillus / Q000378:metabolism / Q000502:physiology; D001628:Beverages / Q000032:analysis*; D002855:Chromatography, Thin Layer; D002957:Citrus / Q000032:analysis*; D005516:Food Microbiology; D011933:Reagent Kits, Diagnostic; D013172:Spores, Fungal / Q000032:analysis; D013997:Time Factors
D000348:Aflatoxins; D011933:Reagent Kits, Diagnostic; D016604:Aflatoxin B1