Assessment of the genotoxic potential of unleaded gasoline and 2,2,4-trimethylpentane in human lymphoblasts in vitro.
The potential of unleaded gasoline and 2,2,4-trimethylpentane to induce gene locus mutation and sister-chromatid exchange in human lymphoblasts in vitro was determined. Neither unleaded gasoline, at its maximum tolerated concentration in the medium, nor 2,2,4-trimethylpentane, at its limit of solubility, induced mutation at the thymidine kinase locus. Negative results were seen both in the presence and absence of a rat liver homogenate metabolizing system. Sister-chromatid exchange analyses of the cultures treated with unleaded gasoline and 2,2,4-trimethylpentane were also negative. Therefore the carcinogenicity and nephrotoxicity of unleaded gasoline and 2,2,4-trimethylpentane observed in vivo are not correlated with any marked genotoxicity in these in vitro tests.
D000818:Animals; D001711:Biotransformation; D002460:Cell Line; D005742:Gasoline / Q000633:toxicity*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D009795:Octanes / Q000378:metabolism / Q000633:toxicity*; D010578:Petroleum / Q000633:toxicity*; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects
D005742:Gasoline; D009153:Mutagens; D009795:Octanes; D010578:Petroleum; C045798:2,2,4-trimethylpentane