title: Screening of agents which convert 'transformed morphology' of Rous sarcoma virus-infected rat kidney cells to 'normal morphology': identification of an active agent as herbimycin and its inhibition of intracellular src kinase.
abstract: During the course of screening of agents active in converting the transformed morphology of Rous sarcoma virus-infected rat kidney cells to the normal morphology, we identified an active substance produced by Streptomyces sp. MH237-CF8 as herbimycin. Herbimycin converted almost all cells into the normal morphology. The antibiotic was found to be an inhibitor of p60src-associated protein kinase in the cells.
mesh_terms: D000818:Animals; D016227:Benzoquinones; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002478:Cells, Cultured; D007668:Kidney; D047029:Lactams, Macrocyclic; D015688:Oncogene Protein pp60(v-src); D009856:Oncogene Proteins, Viral / Q000037:antagonists & inhibitors*; D010658:Phenylglyoxal / Q000031:analogs & derivatives; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D011809:Quinones / Q000494:pharmacology*; D051381:Rats; D012191:Retroviridae Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D017828:Rifabutin / Q000031:analogs & derivatives
chemical_list: D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D009856:Oncogene Proteins, Viral; D011809:Quinones; D012191:Retroviridae Proteins; D017828:Rifabutin; C020754:herbimycin; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src); D010658:Phenylglyoxal; C006697:benzil