A hand-held photometer for quantitative dot-immunobinding assays.
A simple multichannel transmission photometer is presented. This instrument is designed to give a quantitative result from dot-immunobinding assays. The optoelectronic components of the system (yellow GaP light-emitting diode and CdS photoresistor) have been selected to match the absorbance maximum of the product generated by the peroxidase-catalyzed oxidation of 4-chloro-1-naphthol. The standard deviation of duplicate measures is lower than 0.5% on a single channel and lower than 1.5% on different channels. The photometer is able to read linearly the absorbance of samples up to a value of A585 = 3.0. As an application example, the photometer was used to read the results of the titration of sera of Leishmania-infected dogs on Leishmania donovani antigens.
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D004283:Dog Diseases / Q000175:diagnosis; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D004867:Equipment Design; D007136:Immunoglobulins / Q000032:analysis; D007893:Leishmania donovani / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000662:veterinary; D010783:Photometry / Q000295:instrumentation*
D000953:Antigens, Protozoan; D007136:Immunoglobulins