Effect of gamma interferon on spreading of mouse peritoneal macrophages on solid surfaces.
The influence of a homologous gamma interferon preparation (MuIFN-gamma) on in vitro spreading of freshly-seeded unelicited mouse peritoneal macrophages (MPM) was tested. Light microscopy and scanning electron microscopy observations gave comparable results. Addition of MuIFN-gamma enhanced the spreading on solid surfaces, especially 3-5 hours after seeding. Maximal effect was achieved with 10 units of IFN. Addition of specific anti-MuIFN-gamma or treatment of the preparation at pH 2 eliminated both antiviral activity and effect on spreading of MPM.
D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D005898:Glass; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D013997:Time Factors
D007370:Interferon Type I; D007371:Interferon-gamma