An alpha-like DNA polymerase from Halobacterium halobium.
Two DNA polymerases have been isolated from extracts of Halobacterium halobium, one having a sedimentation coefficient of 11 S, designated as alpha-like polymerase and possessing the following characteristics. It is sensitive to both aphidicolin and N-ethylmaleimide but indifferent to the presence of a dideoxynucleoside triphosphate. Therefore this polymerase is very similar to the alpha DNA polymerase of eukaryotes. The enzyme requires 5 M NaCl for maximum activity. The other polymerase has a sedimentation coefficient of 4.4 S and is resistant to both aphidicolin and N-ethylmaleimide. However, it is inhibited by a dideoxynucleoside triphosphate.
D016590:Aphidicolin; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D054306:Dideoxynucleotides; D004224:Diterpenes / Q000494:pharmacology; D006217:Halobacterium / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000494:pharmacology
D054306:Dideoxynucleotides; D004224:Diterpenes; D013942:Thymine Nucleotides; D016590:Aphidicolin; D012965:Sodium Chloride; D004257:DNA Polymerase II; C017216:2',3'-dideoxythymidine triphosphate