Activation of membrane phospholipase C by vasopressin. A requirement for guanyl nucleotides.
Vasopressin stimulates the liberation of labelled inositol phosphate in partially purified plasma membranes prepared from myo-[3H]inositol prelabelled WRK1 cells. This stimulatory effect was very rapid (165% stimulation of inositol trisphosphate accumulation after a 10 s incubation period in the presence of 1 microM vasopressin), concentration dependent (EC50 = 12 nM) and was abolished by an antagonist of the vasopressor response to vasopressin. GTP, even at high concentrations (0.1 mM), did not increase inositol phosphate release: it was found to be absolutely necessary for hormonal stimulation of phospholipase C activity. Non-hydrolysable analogues of GTP may also stimulate this enzyme activity.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006160:Guanosine Triphosphate / Q000502:physiology*; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology*
D007295:Inositol Phosphates; D008563:Membrane Lipids; D008565:Membrane Proteins; D014667:Vasopressins; D006160:Guanosine Triphosphate; D010738:Type C Phospholipases