[Comparison of human tumor clonogenic and nude mouse-isotope assays in anticancer-agent sensitivity tests].
Comparative studies between the in vitro human tumor clonogenic assay (HTCA) and our original in vivo method (NM-IA) in which the final evaluation was made with 3H-TdR incorporation of tumor cells transplanted into nude mice were simultaneously performed on 44 fresh human solid tumors (22 gastric cancers, 6 breast cancers, 4 gall bladder cancers, 4 liposarcomas and 8 other tumors). Mitomycin C (MMC), 5-fluorouracil (5-FU), adriamycin (ADM) and cyclophosphamide (CPM) were tested. The evaluable rate was 73% (32/44) in HTCA and 89% (38/44) in NM-IA. Although correlation between the results of HTCA and those of MN-IA was obtained for MMC and ADM, no correlation was observed for 5-FU and CPM.
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D003114:Colony-Forming Units Assay / Q000379:methods*; D003520:Cyclophosphamide / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005472:Fluorouracil / Q000494:pharmacology*; D005706:Gallbladder Neoplasms / Q000473:pathology; D006801:Humans; D007554:Isotopes; D051379:Mice; D008819:Mice, Nude; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D009368:Neoplasm Transplantation / Q000379:methods; D013274:Stomach Neoplasms / Q000473:pathology; D014410:Tumor Stem Cell Assay / Q000379:methods*
D007554:Isotopes; D008937:Mitomycins; D016685:Mitomycin; D004317:Doxorubicin; D003520:Cyclophosphamide; D005472:Fluorouracil