Identification of the v-rel protein in REV-T transformed chicken bone marrow cells and expression in Cos1 cells.
The product of the v-rel oncogene has been identified as a 55,000 dalton protein using antiserum prepared against a synthetic polypeptide whose sequence was deduced from the DNA sequence of v-rel. This antiserum was used in a Western blot assay to identify the product of v-rel in chicken bone marrow cells transformed with reticuloendotheliosis virus, strain T (REV-T), and in Cos1 cells transfected with an expression vector containing the v-rel gene. Transient expression of v-rel under the transcriptional control of the SV40 late promoter in Cos1 cells leads to the synthesis of a rel specific protein with a similar molecular weight.
D000818:Animals; D000918:Antibody Specificity; D001853:Bone Marrow / Q000378:metabolism; D002460:Cell Line; D002472:Cell Transformation, Viral; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology
D009856:Oncogene Proteins, Viral; D010446:Peptide Fragments