Influence of organic solvents and pH on some characteristics of influenza virus A(H1N1). Note 1. Influence of organic solvents on the spectral characteristics of fluoresceine isothiocyanate-conjugated influenza virus A(H1N1).
The fluorescence of fluoresceine isothiocyanate (FITC)-conjugated influenza virus A(H1N1) was enhanced following addition of ethanol, acetone or dimethylsulfoxide (DMSO) and decreased after addition of dioxane; light scattering values increased in the presence of any of these organic solvents. The effect of the organic solvents could be correlated with the excess values delta epsilon of the dielectric constant. Out of the four organic solvents it was DMSO that caused the slightest changes in the fluorescence and light scattering of the FITC/virus system.
D004305:Dose-Response Relationship, Drug; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000032:analysis / Q000187:drug effects*; D008027:Light; D012542:Scattering, Radiation; D012997:Solvents / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013861:Thiocyanates / Q000494:pharmacology*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D012997:Solvents; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate