Absence of DNA in yolk platelets of Bufo arenarum oocytes.
The absence of DNA in the yolk platelets of Bufo arenarum oocytes was verified. Oocytes from the coelomic cavity were used in order to avoid possible contamination by the ovarian tissue. Four methods for DNA analysis were employed, and the possibility of interference was carefully checked. The presence of three possible contaminants, which produced positive reactions from the DNA analysis, was detected. These were the glycosaminoglycan from the superficial layer of the platelets, sucrose, and polyvinylpyrrolidone from the buffer commonly used during the isolation of platelets. The present evidence points to the necessity of using at least two different methods for DNA determination with due regard for their sensitivity and specificity before any firm conclusion can be reached on the presence or absence of DNA in nonnuclear and mitochondrial parts of the oocyte.
D000818:Animals; D002022:Bufo arenarum; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004530:Egg Yolk / Q000032:analysis*; D005260:Female; D006025:Glycosaminoglycans / Q000302:isolation & purification; D009865:Oocytes / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D012266:Ribose / Q000032:analysis; D013395:Sucrose / Q000032:analysis; D013950:Thymus Gland; D014574:Uronic Acids / Q000032:analysis
D006025:Glycosaminoglycans; D010710:Phosphates; D014574:Uronic Acids; D013395:Sucrose; D012266:Ribose; D004247:DNA