The effect of trifluoperazine (Stellazine) on Hymenolepis diminuta in vitro.
The influence of the phenothiazine trifluoperazine (Stellazine) on the rat tapeworm Hymenolepis diminuta was examined. The parasite was incubated in glucose-containing Krebs-Ringer media (pH 7.4) at 37 degrees C which included Ca2+ or EGTA and a range of trifluoperazine concentrations (0-2 mM). Release of soluble protein and lactate dehydrogenase activity were taken as measures of release of cytosolic components. The release of lactate dehydrogenase depended on drug concentration, maximum levels occurring at 2 mM trifluoperazine, this corresponded to 2% of the total lactate dehydrogenase present in the cestode. The effect of phenothiazines of differing lipophilicity were compared, and for trifluoperazine sulfoxide only minimal amounts of lactate dehydrogenase activity and protein were released. These values were similar to those obtained when H. diminuta was incubated in drug-free media. Our findings suggest that the integrity of the parasite is related to its calmodulin content. The potential cestocidal properties of trifluoperazine are considered.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003470:Culture Media; D003593:Cytoplasm / Q000201:enzymology; D004533:Egtazic Acid / Q000494:pharmacology; D005243:Feces / Q000469:parasitology; D006926:Hymenolepis / Q000187:drug effects* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions; D014268:Trifluoperazine / Q000031:analogs & derivatives* / Q000494:pharmacology*
D003470:Culture Media; D011506:Proteins; C039697:trifluoperazine sulfoxide; D014268:Trifluoperazine; D004533:Egtazic Acid; D007770:L-Lactate Dehydrogenase; D002118:Calcium