Synergistic incorporation of daunorubicin in erythrocytes in the presence of polyene antibiotics. Role of the membrane potential.
The synergistic incorporation into red blood cells of the antitumor compound daunorubicin, in the presence of the polyene antibiotics amphotericin B and vacidin A, depended on the composition of the external medium. Synergism was observed only for concentrations of polyene antibiotics that induce cation permeability. The same synergistic effect was observed with the K+ selective carrier, valinomycin, but this had a different dependence on the external medium composition. By using the membrane probe 3,3'-dipropylthiadicarbocyanine (diS-C3-(5], the synergistic effect was shown to occur under conditions where addition of the ionophores leads to hyperpolarization of the membrane.
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001386:Azides / Q000494:pharmacology; D002021:Buffers; D002174:Candicidin / Q000031:analogs & derivatives / Q000494:pharmacology*; D002412:Cations; D002463:Cell Membrane Permeability / Q000187:drug effects; D003630:Daunorubicin / Q000097:blood*; D004357:Drug Synergism; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D011188:Potassium / Q000097:blood; D019810:Sodium Azide; D014634:Valinomycin / Q000494:pharmacology
D000935:Antifungal Agents; D001386:Azides; D002021:Buffers; D002412:Cations; D002174:Candicidin; D014634:Valinomycin; C031985:vacidin A; D000666:Amphotericin B; D019810:Sodium Azide; D011188:Potassium; D003630:Daunorubicin