[Assessment of the in vitro digestibility of dietary proteins and of the degree of the breakdown of their antigenic structures by using polyenzyme systems].
A comparative assessment was made of the digestion of bovine serum albumin (BSA), chicken ovalbumin (OVA), and casein by means of the gastric juice--duodenal contents floccular gel structures (FGS) system and a four-enzymic system including trypsin, chymotrypsin, peptidase, and bacterial protease preparations. Decomposition of the BSA and OVA antigenic structures with the use of the two systems was also studied. Significant differences in BSA and OVA digestion by the gastric juice--FGS system were detected both with respect to amino nitrogen content and to the degree of their antigenic structure decomposition, whereas no such differences were observed when the four-enzymic system was used. The systems most accurately simulating the 'proteolytic conveyer' conditions of the gastrointestinal tract are preferable for the studies. The developed method is recommended for use in comparative assessment of the nutrient protein sensitizing properties.
D000818:Animals; D000941:Antigens / Q000378:metabolism*; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D002918:Chymotrypsin / Q000378:metabolism; D004044:Dietary Proteins / Q000276:immunology / Q000378:metabolism*; D004063:Digestion; D004285:Dogs; D004386:Duodenum / Q000201:enzymology; D010450:Endopeptidases / Q000378:metabolism; D005750:Gastric Juice / Q000201:enzymology; D006868:Hydrolysis; D066298:In Vitro Techniques; D007419:Intestinal Secretions / Q000201:enzymology; D007422:Intestines / Q000201:enzymology; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology; D013552:Swine; D014357:Trypsin / Q000378:metabolism
D000941:Antigens; D002364:Caseins; D004044:Dietary Proteins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D010450:Endopeptidases; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin
Otsenka perevarivaemosti in vitro pishchevykh belkov i stepeni rasshchepleniia ikh antigennykh struktur s ispol'zovaniem polifermentnykh sistem.