Inflammation-induced stimulation of the synthesis of prostaglandins and lipoxygenase-reaction products in rabbit cornea.
The cyclooxygenase and lipoxygenase pathways that produce prostaglandins (PGs) and hydroxyeicosatetraenoic acids (HETEs) were studied in inflamed rabbit cornea. A cryogenic lesion was induced and five days later the epithelium, stroma and endothelium were isolated and incubated with [1-14C]arachidonic acid. After lesioning, the arachidonic acid metabolites, thromboxane B2, PGF2 alpha and 6-keto-PGF1 alpha exhibited the greatest increase in the stroma. Lipoxygenase products were formed in the three layers also, although 12-HETE predominated. The correlation between the synthesis of these compounds and corneal injury is discussed.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002250:Carbon Radioisotopes; D003315:Cornea / Q000378:metabolism; D003319:Corneal Stroma / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005615:Freezing; D007634:Keratitis / Q000201:enzymology / Q000209:etiology / Q000378:metabolism*; D008084:Lipoxygenase / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D011817:Rabbits
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D011453:Prostaglandins; D016718:Arachidonic Acid; D008084:Lipoxygenase