VH-gene expression in murine lipopolysaccharide blasts distributes over the nine known VH-gene groups and may be random.
VH-gene expression in hybridomas derived from lipopolysaccharide-activated B cells was analyzed. Isolated cytoplasmic RNA was hybridized to probes representing the 9 known VH-gene groups or subjected to mRNA sequencing. In the collection of hybridomas VH genes of all 9 groups are expressed at frequencies which in most correlate reasonably well with the relative complexities of the groups. In 51 out of 54 RNA samples VH-gene transcripts could be identified and corresponded to one of the known VH-gene groups. It therefore appears that the latter essentially represent the VH-gene cluster of the mouse.
D000818:Animals; D002460:Cell Line; D005787:Gene Frequency; D005796:Genes; D006825:Hybridomas / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008070:Lipopolysaccharides