Promoter specificity of a sporulation-induced form of RNA polymerase from Bacillus subtilis.
The program of gene expression that underlies endospore formation by Bacillus subtilis may be controlled in part by a sporulation-induced form of RNA polymerase, E sigma 29. The nucleotide sequences of four promoters, which are known to be recognized by E sigma 29, are highly conserved at two regions, 10 bp and 35 bp upstream from the start point of transcription. We have used oligonucleotide-directed mutagenesis to construct several base substitutions in the ctc promoter from B. subtilis to test the role of the highly conserved sequences in utilization of the promoter by E sigma 29. In vitro transcription analysis demonstrated that the conserved nucleotides at positions -15, -14 and -12 affect the utilization of the promoter by E sigma 29. These and previous results support a model in which E sigma 29 recognizes its cognate promoters by interacting with nucleotides near the -10 and -35 regions. We also examined the effects of these base substitutions on utilization of the promoter by two other forms of RNA polymerase from B. subtilis, E sigma 37 and E sigma 32.
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D011401:Promoter Regions, Genetic; D013171:Spores, Bacterial / Q000201:enzymology; D013698:Templates, Genetic; D014158:Transcription, Genetic
D012321:DNA-Directed RNA Polymerases