Role of chloride on carrier-mediated transport of p-aminohippurate in rat renal basolateral membrane vesicles.
Effect of inorganic anions on p-amino[3H]hippurate transport in renal basolateral membranes has been studied using the vesicles preloaded with unlabeled p-aminohippurate (countertransport condition). The uptake of p-amino[3H]hippurate was stimulated by the outward gradient of unlabeled p-aminohippurate and the labeled substrate was accumulated into the vesicles against its concentration gradient in the presence of Cl-. The substitution of SCN- and SO4(2-) for Cl- in both sides of the vesicles depressed the initial rate and the overshoot magnitude of p-amino[3H]hippurate uptake. These results suggest that Cl- may play an important role for the carrier-mediated transport system of organic anion in renal basolateral membranes.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism; D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D007672:Kidney Cortex / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D002712:Chlorides; D012965:Sodium Chloride; D011189:Potassium Chloride; D010130:p-Aminohippuric Acid