title: Metabolism of benzo(a)pyrene in the nuclear and microsomal fractions from rhesus monkey liver.
abstract: The metabolism of benzo(a)pyrene was studied in the nuclear and microsomal fractions from four rhesus monkey livers. The nuclear fraction was obtained by differential centrifugation, including a step in 2.3 M sucrose. The metabolites from 3H-benzo(a)pyrene were separated by high performance liquid chromatography (HPLC) and the radioactivity was measured. The nuclear fraction catalyzed the formation of 4,5- and 7,8-dihydrodiols and that of 9-OH and 3-OH phenols. The ratio between the nuclear and microsomal rate of formation of the metabolites ranged between 0.02 and 0.03. The nuclear fraction did not catalyze the formation of 9,10-dihydrodiol or quinones at a detectable rate. In the microsomal fraction there was a preferential formation of 4,5-dihydrodiol and 3-OH phenol. Nuclei differ from the microsomes for the lack of formation of quinones.
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008253:Macaca mulatta; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism
chemical_list: D011809:Quinones; D001564:Benzo(a)pyrene; D001189:Aryl Hydrocarbon Hydroxylases