[Spectrofluorimetric method of determining epoxide hydrolase].
A spectrofluorometric procedure is developed for estimation of epoxide hydrolase activity using styrol oxide as a substrate. The data were plotted, reflecting the dependence of the enzymatic reaction on time, pH value and the substrate concentration. Activity of epoxide hydrolase was studied in various tissues and subcellular fractions of rat liver tissue.
D000818:Animals; D004851:Epoxide Hydrolases / Q000032:analysis*; D004852:Epoxy Compounds / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution
D004852:Epoxy Compounds; C013690:styrene oxide; D004851:Epoxide Hydrolases
Spektrofliuorimetricheskiĭ metod opredeleniia aktivnosti époksidgidrolazy.