Activation of the alternative complement pathway by mumps infected cells: relationship to viral neuraminidase activity.
An inverse relationship exists between the sialic acid content of a particle and its ability to activate the alternative complement pathway. The present studies were performed to determine if the neuraminidase (NANase) activities of different mumps virus strains could influence the ability of mumps virus infected cells to activate the alternative pathway. CV-1 cells were infected with three different mumps virus strains (RW, O'Take, and Kilham) and after 24 hours, 10 percent guinea pig serum (GPS) treated with EGTA/MgCl2 or GPS lacking the 4th component of complement (C4DGPS) was added to the cell monolayers. After 30 minutes, the percentage C3 consumed was determined by a functional hemolytic assay. Cells infected with RW (high NANase) consumed significantly more C3 (23.2 per cent) than cells infected with Kilham (5.7 percent, low NANase). Cells infected with O'Take were intermediate in their ability to activate C3. The degree of C3 deposition on the surface of infected cells, detected by fluorescence microscopy, was also greater for cells infected with the RW than the Kilham strain of mumps virus. These studies suggest that the NANase activity of mumps virus can influence the ability of infected cells to activate the alternative pathway and thereby, the ability of complement to participate in host defense against mumps virus infection.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002522:Chlorocebus aethiops; D003167:Complement Activation; D003176:Complement C3 / Q000276:immunology; D003170:Complement Pathway, Alternative; D009107:Mumps / Q000276:immunology; D009109:Mumps virus / Q000201:enzymology / Q000276:immunology*; D009439:Neuraminidase / Q000502:physiology*
D000956:Antigens, Viral; D003176:Complement C3; D009439:Neuraminidase