Autologous survival of cyanate-treated cryopreserved sickle erythrocytes.
The effects of carbamylation and frozen storage on the autologous 51Cr survival and metabolic features of sickle erythrocytes (S-RBCs) were determined. Red cells from four patients with sickle hemoglobinopathies were treated with 50 mM sodium cyanate for 2 hr (37 degrees C), glycerolized and frozen (-80 degrees C) for 62-153 days. The mean in vitro loss of S-RBCs from the combination of cyanate treatment and cryopreservation was 23.6% ( +/- 3.5 SD). The 2,3-diphosphoglycerate content of the thawed cells did not change significantly. However, ATP levels decreased to about 50% of the corresponding values in fresh, untreated S-RBCs. Despite this decrease in ATP, the mean intravascular survival of the frozen cyanated cells nearly doubled. At the high concentration of cyanate used, the oxygen affinity of S-RBCs increased markedly: Their mean P50 was 13.1 mm Hg ( +/- 1.9SD). The gelation of HbS at zero pO2 was also markedly inhibited in the one sample of cyanate-treated S-RBCs examined. Clinical studies to determine the efficacy of autologous transfusions with extensively carbamylated, cryopreserved S-RBCs should be considered.
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000032:analysis; D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood / Q000473:pathology*; D001793:Blood Preservation; D003485:Cyanates / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000032:analysis; D004905:Erythrocyte Aging / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005615:Freezing; D006801:Humans; D010100:Oxygen / Q000378:metabolism
D003485:Cyanates; D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate; C009281:sodium cyanate; D010100:Oxygen