title: Molecular specificity of ganglioside effects on neurite regeneration of sensory neurons in vitro.
abstract: Highly purified preparations of individual gangliosides have been tested for their ability to modulate the survival and morphological differentiation of embryonic chick dorsal root ganglion neurons. When added at 48 h to established cultures of nerve growth factor (NGF)-dependent neurons, all ganglioside species tested increased the expression of neurofilament protein. Poly- and trisialogangliosides were more effective than di- or monosialogangliosides. In contrast, neither NGF nor an antiserum against NGF influenced neurofilament protein expression over this period of culture. In addition, ganglioside-induced expression of neurofilament protein was not inhibited by the anti-NGF serum.
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000502:physiology; D007381:Intermediate Filament Proteins / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D016900:Neurofilament Proteins; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D007381:Intermediate Filament Proteins; D009414:Nerve Growth Factors; D016900:Neurofilament Proteins; D005677:G(M1) Ganglioside