Influence of clonogenic assay methodology on measurement of drug sensitivities in vitro.
To assess the influence of clonogenic assay methodology on measurement of reproductive cell kill, the in vitro sensitivities of a human bladder cancer cell line, RT112, to methotrexate and adriamycin were determined using ten different procedures. Marked differences in dose-response to methotrexate, but not adriamycin, were observed.
D000970:Antineoplastic Agents; D002460:Cell Line; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity; D006801:Humans; D008727:Methotrexate / Q000633:toxicity; D014411:Neoplastic Stem Cells / Q000187:drug effects; D014410:Tumor Stem Cell Assay; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy / Q000473:pathology
D000970:Antineoplastic Agents; D004317:Doxorubicin; D008727:Methotrexate