title: Effects of chemical sympathectomy on dopamine and noradrenaline content of the dog gastrointestinal tract.
abstract: The content of dopamine and noradrenaline in the mucosa-submucosa and muscular layers of different gastrointestinal areas of the dog, and its modification by 6-hydroxydopamine or pargyline plus 6-hydroxydopamine was studied by means of high pressure liquid chromatography with electrochemical detection. The amounts of dopamine and noradrenaline show a cephalocaudal increase but their physiological levels were rather low when compared with those reported in other tissues. This finding was consistent with the sparse noradrenergic innervation classically described for the gut with histochemical methodologies. On the basis that noradrenergic neurones are considered more susceptible to 6-hydroxydopamine than dopaminergic neurones, a difference abolished by previous treatment with pargyline, our findings did not suggest the existence of dopaminergic neurones in the gastrointestinal tract of the dog. In the muscular layer of the duodenum, jejunum and ileum it was observed that catecholamine depletion by both treatments was smaller than that obtained in the mucosa-submucosa. Due to the resistance to both kinds of chemical sympathectomy exhibited by the dopamine content, the existence of dopamine-containing enterochromaffin cells is proposed in the mucosa-submucosa of different portions of the stomach and small intestine.
mesh_terms: D000818:Animals; D004064:Digestive System / Q000378:metabolism*; D004285:Dogs; D004298:Dopamine / Q000378:metabolism*; D004947:Esophagus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006892:Hydroxydopamines / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D016627:Oxidopamine; D010293:Pargyline / Q000494:pharmacology; D013563:Sympathectomy, Chemical
chemical_list: D006892:Hydroxydopamines; D016627:Oxidopamine; D010293:Pargyline; D004298:Dopamine; D009638:Norepinephrine