Gene expression of ornithine decarboxylase in L1210 leukaemia cells exposed to DL-2-difluoromethylornithine in the presence of cadaverine.
Cultured mouse L1210 leukaemia cells treated with DL-2-difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase (EC 4.1.1.17), in the presence of micromolar concentrations of cadaverine, started to overproduce ornithine decarboxylase after an exposure of several weeks. The more than 60-fold excess of the enzyme protein in the drug-treated cells apparently resulted from a strikingly enhanced accumulation of mRNA for the enzyme associated with only a modest (about 2-fold) gene amplification.
D000818:Animals; D002103:Cadaverine / Q000494:pharmacology*; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000235:genetics; D003959:Diamines / Q000494:pharmacology*; D000518:Eflornithine; D005784:Gene Amplification / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D007939:Leukemia L1210 / Q000235:genetics*; D051379:Mice; D009952:Ornithine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000235:genetics*; D065108:Ornithine Decarboxylase Inhibitors; D012333:RNA, Messenger / Q000235:genetics
D004273:DNA, Neoplasm; D003959:Diamines; D065108:Ornithine Decarboxylase Inhibitors; D012333:RNA, Messenger; D009952:Ornithine; D009955:Ornithine Decarboxylase; D002103:Cadaverine; D000518:Eflornithine