Biohybrid artificial pancreas. Long-term insulin secretion by devices seeded with canine islets.
Long-term insulin secretion was investigated in seven biohybrid capillary devices seeded with canine islets. Approximately 50,000 islets could be isolated from a single canine pancreas using collagenase digestion in conjunction with the recently described Velcro technique. Devices were perfused with tissue culture medium 199 containing 300 mg/dl glucose. Insulin secretion fell during the first 1-2 days of culture from approximately 18 to 6 U/day. After 3-4 wk of perfusion, however, there was a gradual rise in insulin output that reached greater than 15 U/day after 7 wk. Insulin output eventually stabilized at 18-20 U/day. Two of these devices were studied for 80 and 86 days, respectively, and continued to secrete these same amounts of insulin. Glucose-induced insulin release was studied after 1 and 4 wk of culture and was well preserved.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004285:Dogs; D005260:Female; D005947:Glucose / Q000378:metabolism; D000882:Haplorhini; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007332:Insulin Infusion Systems; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D013997:Time Factors
D003470:Culture Media; D007328:Insulin; D005947:Glucose