Immune memory expression to Tnp-Ficoll in CB.20 mice: evidence for a multigenic control.
Trinitrophenyl (Tnp)-Ficoll, a class 2 thymus-independent (TI) antigen, generates in most mouse strains Tnp-specific B-memory cells which can be detected in situ 1 week after priming by a heterologous stimulation with Tnp-lipopolysaccharide (LPS) but not by a homologous Tnp-Ficoll challenge. We have investigated the secondary responses raised in CB.20 congenic mice by a homologous challenge in situ occurring at various time intervals after priming. We report that a memory-type response is obtained, culminating when the challenge is performed at 4 weeks; this finding assesses definitely the ability of TI-2 antigens to produce immunological memory under standard conditions. However, the same immunization procedure elicits no memory-type response in the majority of other mouse strains, suggesting a possible genetic control of the expression of memory to class 2 TI antigens. The utilization of F1 hybrids between C57BL/6 and BALB/c and of appropriate congenic strains shows indeed that this memory expression is under multigenic control: Igh-V or closely linked genes are clearly involved but a complementation with other gene(s), located outside the H-2 complex, is required for a memory-type response to Tnp-Ficoll. We have also analyzed the secondary heterologous response to Tnp-LPS in CB.20 mice at different times after Tnp-Ficoll priming. The difference in the kinetic profile of the heterologous (TI-2----TI-1) versus homologous (TI-2----TI-2) secondary responses is discussed in terms of B-memory-cell ontogeny and humoral regulation.
D000818:Animals; D000955:Antigens, T-Independent / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005362:Ficoll / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000276:immunology*; D005796:Genes; D005816:Genetic Complementation Test; D007117:Immunization, Secondary; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009578:Nitrobenzenes / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D013045:Species Specificity; D014301:Trinitrobenzenes / Q000008:administration & dosage / Q000276:immunology*
D000955:Antigens, T-Independent; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D009578:Nitrobenzenes; D011134:Polysaccharides; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll