External ATP permeabilizes transformed cells to macromolecules.
External ATP under certain ionic conditions render transformed cells permeable to the translation inhibitor hygromycin B. With this method the protein toxin alpha-sarcin selectively penetrates into 3T6 cells, as compared to 3T3 cells. This entry is enhanced by ATP synthesis blockers such as CCCP. Other proteins, such as horseradish peroxidase and luciferase, also pass selectively in 3T6 cells under permeabilization conditions.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D004722:Endoribonucleases; D005656:Fungal Proteins / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D006921:Hygromycin B / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D011506:Proteins / Q000378:metabolism*
D005656:Fungal Proteins; D011506:Proteins; C014801:alpha-sarcin; D006921:Hygromycin B; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000255:Adenosine Triphosphate; D006735:Horseradish Peroxidase; D008156:Luciferases; D004722:Endoribonucleases