Expression of Ro/SS-A antigen in human skin and heart.
Using human sera monospecific for anti-Ro/SS-A antibodies in an indirect immunofluorescence assay, Ro/SS-A antigen was found to be present in nuclei of human fetal ectodermal cells and newborn epidermal keratinocytes, but not in adult epidermal keratinocytes. After in vitro cultivation, Ro/SS-A antigen was also found in the nuclei of adult skin explant outgrowth cells. The localization of Ro/SS-A in fetal heart could not be precisely determined (possibly nuclear and membrane). Extracts from fetal skin and heart contained Ro/SS-A antigen which is identical to the Ro/SS-A present in WiL2 cell extract. The molecular weight of Ro/SS-A antigenic peptide present in these extracts is around 60K.
D000328:Adult; D000375:Aging; D000941:Antigens / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis*; D003378:Counterimmunoelectrophoresis; D005260:Female; D005318:Fetal Heart / Q000276:immunology; D005333:Fetus; D006801:Humans; D005779:Immunodiffusion; D007231:Infant, Newborn; D008297:Male; D009206:Myocardium / Q000276:immunology*; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D012867:Skin / Q000276:immunology*; D000099207:SS-A Antigen
D000941:Antigens; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human