Site-specific and bulk-phase generation of hydroxyl radicals in the presence of cupric ions and thiol compounds.
Addition of a thiol compound to a solution containing Cu2+ and H2O2 resulted in the generation of hydroxyl radicals (OH.). These radicals were able to oxidize salicylic acid and tryptamine in a reaction that was strongly inhibited by the OH.-scavenger mannitol. Covalent coupling of the thiol compound to tryptamine did not significantly influence the degradation of the indole moiety subsequent to addition of H2O2 and Cu2+. The inhibiting effect of mannitol, however, was strongly reduced, indicating that the scavenger could not interfere with site-specific reactions of OH..
D003300:Copper / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006900:Hydroxylation; D008353:Mannitol / Q000494:pharmacology; D012459:Salicylates / Q000378:metabolism; D020156:Salicylic Acid; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D014363:Tryptamines / Q000378:metabolism
D005290:Ferric Compounds; D005609:Free Radicals; D012459:Salicylates; D013438:Sulfhydryl Compounds; D014363:Tryptamines; D008353:Mannitol; C030820:tryptamine; D003300:Copper; D004492:Edetic Acid; D006861:Hydrogen Peroxide; C019179:Fe(III)-EDTA; D020156:Salicylic Acid