title: Dual adhesion systems of chick myoblasts.
abstract: Cultured chick myoblasts (Mb) were resuspended by incubation with 100 micrograms/ml trypsin/2.5 mM CaCl2 (to yield TC-Mb), or with 5 micrograms/ml trypsin/2.5 mM EDTA (to yield LTE-Mb). As measured in a particle counter, TC-Mb aggregation was Ca2+ dependent, whereas LTE-Mb aggregated equally well in the presence of CaCl2 or EDTA. Cells subjected to the same treatments in sequence, like cells dissociated directly with 100 micrograms/ml trypsin/2.5 mM EDTA, did not aggregate significantly in the presence or absence of Ca2+. Adhesive specificity was assessed by mixing unlabeled cells with cells labeled with a fluorescent dye and then analyzing the distribution of fluorescent and nonfluorescent cells in aggregates. No adhesive specificity was seen in controls (i.e., TC-Mb aggregated randomly with TC-Mb, or LTE-Mb with LTE-Mb), but TC-Mb and LTE-Mb did not cross-adhere. These results indicate the existence of two independent, noncomplementing, adhesion systems, and suggest that the differential treatments preserve or activate one system while destroying the other. Myoblasts dissociated with 2.5 mM EDTA in the absence of exogenous trypsin (E-Mb) have both adhesion systems active on their surfaces, as do Mb grown in Ca2+-free medium and then dissociated with 0.7 mM EDTA (Knudsen, K. A., and Horwitz, A. F., Dev. Biol. 58, 328-338, 1977). Although aggregation of E-Mb is largely Ca2+ independent and that of Knudsen/Horwitz-Mb is largely Ca2+ dependent, they adhere well to each other and to LTE-Mb while segregating from TC-Mb. Fibroblasts also have dual adhesion systems, one Ca2+ dependent and the other Ca2+ independent, but TC-Fb do not cross-adhere to TC-Mb (nor E-Fb to E-Mb). Cell type-specific adhesive selectivity may thus contribute to the selectivity of myocyte fusion.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D004492:Edetic Acid / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009132:Muscles / Q000166:cytology* / Q000196:embryology; D013861:Thiocyanates; D014357:Trypsin / Q000494:pharmacology
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D004492:Edetic Acid; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate; D002118:Calcium