Involvement of Ca2+ in platelet-derived growth factor-induced expression of c-myc oncogene in Swiss 3T3 fibroblasts.
Addition of platelet-derived growth factor (PDGF) to quiescent cultures of Swiss 3T3 fibroblasts rapidly increased the cytoplasmic free Ca2+ concentrations and afterwards the c-myc mRNA levels. The concentrations of PDGF needed for both reactions were roughly the same. Moreover, the Ca2+ ionophores, A23187 and ionomycin, mimicked the actions of PDGF and increased the c-myc mRNA levels. These results suggest that Ca2+ may be involved in the regulation of PDGF-induced expression of the c-myc gene.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004987:Ethers / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D015759:Ionomycin; D051379:Mice; D009857:Oncogenes; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
D004987:Ethers; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D000001:Calcimycin; D015759:Ionomycin; D002118:Calcium