Stimulation of interleukin-3 dependent cell growth by specific antibody.
We are attempting to raise antibodies against interleukin-3 receptors by immunizing mice with one cloned line of IL-3 dependent cells. In syngeneic mice immunized with a basophil cell line AC-2, serum contains stimulatory activity preferential for the immunizing cells but largely inactive against an alternative IL-3 dependent line of different phenotype. The serum activity corresponds to a fraction containing a cell agglutinin. Supernatants from hybridomas constructed from immune spleen cells raised by direct spleen immunization also contain binding antibodies, of which a select number show phenotype-restricted proliferative activity. We suggest that such antibodies may be recognizing receptors for IL-3, and that phenotypically different IL-3 dependent cells may express different membrane epitopes concerned with interacting with IL-3.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D001491:Basophils / Q000276:immunology; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology; D007377:Interleukin-3; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011971:Receptors, Immunologic / Q000276:immunology*; D016185:Receptors, Interleukin-3
D000906:Antibodies; D007377:Interleukin-3; D008222:Lymphokines; D011971:Receptors, Immunologic; D016185:Receptors, Interleukin-3