Eosinophil adherence to infective larvae of Trichinella spiralis: quantification and modulation.
Eosinophils play an important role in host defence against helminthic infection. A quantitative system for studying the interactions of eosinophils and a large non-ingestible target was developed using infective muscle-stage larvae of Trichinella spiralis. At 37 degrees, this antibody-dependent system provided a reliable, reproducible index of eosinophil function. Adherence was equivalent under aerobic and anaerobic conditions. No significant differences were noted between peritoneal exudate eosinophils from Trichinella-infected and normal animals. Adherence was inhibited by leukotriene inhibitors [eicosaetraynoic acid (10(-3) M), nordihydroguaiaretic acid (10(-4) M), and BW755C (10(-3) M)] but not by prostaglandin inhibitors (aspirin and indomethacin), suggesting that leukotrienes mediate this eosinophil function. This assay provides another useful method for assessment of a crucial eosinophil function.
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002396:Catechols / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D004804:Eosinophils / Q000276:immunology*; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D007814:Larva; D008297:Male; D009637:Masoprocol; D051379:Mice; D010101:Oxygen Consumption; D011720:Pyrazoles / Q000494:pharmacology; D011817:Rabbits; D013696:Temperature; D014235:Trichinellosis / Q000276:immunology*
D000906:Antibodies; D002396:Catechols; D011720:Pyrazoles; D015117:5,8,11,14-Eicosatetraynoic Acid; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D009637:Masoprocol; D006632:Histamine