title: Combination antitumor therapy with rabbit tumor necrosis factor and chemo- and immuno-therapeutic agents against murine tumors.
abstract: The antitumor activity of partially purified rabbit tumor necrosis factor (TNF) in combination with lentinan or chemotherapeutic agents was tested. Partially purified TNF was obtained from TNF-containing rabbit sera by salt precipitation and ion-exchange chromatography. Its specific activity, determined in vitro as its cytotoxic activity against L929 cells in the presence of actinomycin D, was 10(5) U/mg protein or about 30-fold that of the crude rabbit sera. The growths of MH134 hepatoma in C3H/He mice and Lewis lung carcinoma in C57BL/6 mice were partially inhibited by intratumoral administration of a suboptimal dose of the TNF preparation. Additional treatment with lentinan, actinomycin D, mitomycin C or adriamycin significantly increased the antitumor activity. In particular, combination therapy with TNF and lentinan was very effective against MH134 hepatoma without having detectable side-effects, showing that lentinan expanded the therapeutical potential of TNF. These results are discussed in relation to our previous results on combination antitumor therapy.
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D003609:Dactinomycin / Q000627:therapeutic use; D004317:Doxorubicin / Q000627:therapeutic use; D006023:Glycoproteins / Q000008:administration & dosage / Q000627:therapeutic use*; D006131:Growth Inhibitors / Q000627:therapeutic use*; D007912:Lentinan / Q000627:therapeutic use*; D008114:Liver Neoplasms, Experimental / Q000628:therapy; D008175:Lung Neoplasms / Q000628:therapy; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D016685:Mitomycin; D008937:Mitomycins / Q000627:therapeutic use; D009374:Neoplasms, Experimental / Q000628:therapy*; D011134:Polysaccharides / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D000970:Antineoplastic Agents; D006023:Glycoproteins; D006131:Growth Inhibitors; D008937:Mitomycins; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D003609:Dactinomycin; D007912:Lentinan; D016685:Mitomycin; D004317:Doxorubicin