Ecdysterone selectively stimulates the expression of a 23000-Da heat-shock protein-beta-galactosidase hybrid gene in cultured Drosophila cells.
To study the regulated expression of cloned heat-shock genes in homologous cells, hybrid Drosophila heat-shock-Escherichia coli beta-galactosidase genes were constructed. Segments of the ecdysterone-inducible 23,000-Da heat-shock protein (hsp23) gene and of two other hsp genes (hsp84 and 70), which are not hormone regulated, were functionally linked to the bacterial coding sequence, and the resulting hybrid genes were introduced into cultured, hormone-responsive Drosophila cells by transfection. All hybrid genes directed the synthesis of E. coli-specific beta-galactosidase in heat-treated cells. hsp23 hybrid gene expression was stimulated strongly by ecdysterone, while the activities of the other hybrid genes were not affected at all by the hormone. A hybrid gene with only 147 bp of hsp23 promoter sequence could not be activated by either heat or ecdysterone treatment. Thus, far upstream sequences contain signals required for the regulated expression of the hsp23 gene in Drosophila cells.
D000818:Animals; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D004441:Ecdysterone / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D005696:Galactosidases / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006824:Hybridization, Genetic; D013045:Species Specificity; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
D006360:Heat-Shock Proteins; D004441:Ecdysterone; D005696:Galactosidases; D001616:beta-Galactosidase